LOCUS       DAOQYN010000001        34210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_1478_length_34210_cov_5.323994, whole genome shotgun sequence.
ACCESSION   DAOQYN010000001 DAOQYN010000000
VERSION     DAOQYN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 34210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34210
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1478_length_34210_cov_5.323994"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..513
                     /locus_tag="PKN80_00005"
     CDS             1..513
                     /locus_tag="PKN80_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNQ34436.1"
                     /translation="MLAGWIGLYNMFILLKNNVARAWSNIDVLLKQRYDELPKLVEVC
                     RSYMEYEKETLERVIRLRSVIGGASGLEERLKAEGELAGALKTLFAVSENYPQLKANE
                     NFEQLRQRISGLENEIADRREFYNESANLYNIKIAQIPDVLVARMMGCKPIPLFETAP
                     EQRESSPLNI"
     gene            540..1685
                     /locus_tag="PKN80_00010"
     CDS             540..1685
                     /locus_tag="PKN80_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNQ34437.1"
                     /translation="MWKSRLFTPGPTAVPDEVLLEMARPLIHHRTPQFRKLLKEAFEG
                     LKYVFQTGQDVMILTSSGTGAMEAAVANLLSPGDHCLVVEGGKFGERFRELAQAYGAA
                     VKTIPVEWGRSVSAGAIEAALKSDPAVKVVFTTLCETSTGAHLDLKAIGAAVRQHPET
                     LLVVDAISSLGAVECRMDEWGVDALISASQKAFMLPPGLAFIALSPKAWKAVEAARSP
                     RYYFDLSAYRQTLAKEDTPYTPALTLVIGLVRVLRMMKEEGLENVWRRHRILAEATRE
                     AMRALGLKLLAEHPSDGLTAVLLPEGLDAEAMVKALRDQSGITFAGGQGKLKGRIFRI
                     AHMGWVDGFEVLEAIAAVEIQLARLGYGLTPGAGVAAAEKVLLSAGG"
     gene            1728..3317
                     /gene="serA"
                     /locus_tag="PKN80_00015"
     CDS             1728..3317
                     /gene="serA"
                     /locus_tag="PKN80_00015"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNQ34438.1"
                     /translation="MEAKQIRVLVTDPLSKEGVAILKNEGFEVEETGKLTPAELAGKV
                     KGFDALVVRSGTRVNREAIAAADNLKVIGRAGVGLDNVDLESATAKGIIVMNSPEGNT
                     ISTAEHAFALILSMMRNIPEAHHSVREGRWEKKGFTGTELYRKVLGIIGFGRIGQRVA
                     QYGRVFGMEVLIYDPFVSEEKALQAGVNLVDQEHLLKNADVITLHLPLSEETRDLIGA
                     RELALMKKGAFLVNCARGGLVNEPALIEALKSGHLKAAALDVFSEEPPADPAFRETPR
                     LVLTPHLGASTEEAQVNVARDICRQIVDYFRKGIISNAANLPTISEKVLEYLRPYLNL
                     CEKLGLFLAQTAPGRINEVKLEYYGDMASFDLSPLHHSFLKGLLSQMGAGVNYINAPV
                     MAQERGIQFAEQKSTISQEFANLITATVSDSGSSISVSGTLFGKTEPRLVKIDGYRCE
                     AVPEGYLLFCRNEDKPGIVGQIGTLLASRQINIAGMTLGRKERGGLAITILNVDEMVD
                     EPLRERIRQIKEIKSVSLIKL"
     gene            3575..5113
                     /gene="rny"
                     /locus_tag="PKN80_00020"
     CDS             3575..5113
                     /gene="rny"
                     /locus_tag="PKN80_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003385780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNQ34439.1"
                     /translation="MEILLGLLGLGLGYLIHLSITSRNLKASEARSQGLLKEAEREIS
                     QRRKELELEVKESLIKQRGDFERETREERRQLQVLEQRLNQRESNLDRKLNLVDKKET
                     DLNQREKTLADQEKKVADRQTELDGVLEEEKRRLSQVAGLSQDEARRILIARMEDDAR
                     HEAALRIKRIEEETREEANNISRTILLEAIQRCASEQAESSTTSVVSLPSDEMKGRLI
                     GREGRNIRAFEAATGVDLIVDDTPEAVTISAFNIYRREIARLVLKRLISDGRIHPGRI
                     EEVVEKVKAEMEEETLKVGKEAALSLGIHKINPELLRMLGRLKYRTSYGQNVLEHSIE
                     VAHLAGTMAGELKLDQTMAKRAGLLHDIGKSVDQEMEGDHLEIGYEIASRYDESKEVL
                     EAIRGHHDDFRPTAPYAVLISAADALSASRPGARRETLEGYIKRLEKLEAIATGFRGV
                     NQAYAISAGREVRVIVKPEAMSDDEASLLARQISGKIEEGLEYPGTIKVTVIREARYS
                     EMAK"
     gene            5125..6198
                     /locus_tag="PKN80_00025"
     CDS             5125..6198
                     /locus_tag="PKN80_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ34440.1"
                     /translation="MKSYRELWRDIMFYRGHDRMPVVHWRGWPETRERWLKEGLPPDV
                     EEMEYFRAVPTGHSVSVELGLRPGFTEETIEETAEYRVFRDASGVVQKAWKHQSNIPH
                     YTDFTFKTARDWPEYKKRLQPGPERIPADLDEKLRAAEVRDCPIIIRICSLMGWVRNW
                     MGVENFAYLMYDDPDVYADVVNTLADLSCWGIDQVVPRLKVKPDLAFGWEDISGKSGP
                     FVSPSVFKRCVAPGYRKIRDKLESHGIHLMAVDSDGDGRALIGPWLEAGVNIQFPVEI
                     GTWQTDPMELRRRYGRELRIIGGYNKLALEKGRAAIDAELERRLPLMKEGGFLLAPDH
                     LITPGVPLADYLYYLDRVRQLRF"
     gene            6215..7198
                     /locus_tag="PKN80_00030"
     CDS             6215..7198
                     /locus_tag="PKN80_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ34441.1"
                     /translation="MEYIAGVDIGGTKIAATLFRPDGELLAKVVFPTRVERGPEAALA
                     EIRTRLEELRRGRPAERLLGIGISCGGPLDSRTGLILSPPNLPGWASVPVTRFLSEAF
                     RVPARLENDANAGALAEFRFGAGRASAASGRPVRNLVFMTMGTGIGAGLILDGRLYRG
                     ATGLAGEIGHTKIIPGGPECGCGGRGCLEALAAGPAIARRARERRKEFPASRIWTLAG
                     GRPEAVTAAEVFAAAAAGDRLGRLVVEETAYYLGLGLANLVNLLNPEVIVLGTIATRY
                     GDFFLEPVRRVVAAEAWPDPVAAVRITGSPLGDLTGDYAALAVFLDQSPGA"
     gene            7351..8553
                     /locus_tag="PKN80_00035"
     CDS             7351..8553
                     /locus_tag="PKN80_00035"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNQ34442.1"
                     /translation="MAGNLILAQSGGPTAVINGSIAGVVMEALRHKEIGGIYGAVNGI
                     EGVLDQNLVDLRAEEPEVISGLRSTPSAALGSCRRKLKADDYQKILDVLKKYEVRYFL
                     IAGGNDSMDTALQMHKLARSSGYELFSVGVPKTVDNDLRVTDHCPGYGSVARYWAISG
                     RDAGRDTDAIYHPDTVKILETMGRDTGWITAAAGLACEAGDDAPQLVYVPEIPFDQEQ
                     FLGDVDRVYRKLGRVVIAVCEGLKDKSGEFVTASGRKTDTDSFGHKQLGGVGDVLCDL
                     IAEKLGLKSRFDKPGTIQRMAMVCASEVDREEAYQVGKQAVRLALAGRSGVMVTIERQ
                     PGAEYRVAYGTGDLTEIANLRKDLPREFMNAAGNHVSQAFLDYARPLAGGPLPPYVRL
                     KKVAVPKR"
     gene            8589..10520
                     /locus_tag="PKN80_00040"
     CDS             8589..10520
                     /locus_tag="PKN80_00040"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNQ34443.1"
                     /translation="MIKERFAAERLSDEQLRELAELSRVCKGDILKMTTLAACGHPGG
                     SISSLDLYLVLFKYAAVDPGDPDNPNRDHILISHGHTSPGAYATLGRLGFFSIEAAVS
                     GFRKIDTPFEGHVVRELPGLDWSSGNLGQGLSAGCGFALASRILKRGFHVFVAMSDGE
                     HAKGQIAESRRFAAKYGLDNLTVVIDYNHIQISGKIEDVMPQNIKGNYLADGWRVLEV
                     DGHDHQAIYQACREAASDGKPTVILAETVMSRGISFMEGLPKYHGAALKPDDCRKALA
                     ELGLEDDLDRLGQLRKNWSFQVPVRRFEYPVSLNLGEPVLYPAGSGADNRGAFGKALL
                     SIASASCGSDDKTPLVVVDCDLATSVRTEDFAKLAPCSFYEAGVQEHTAATLSGALSA
                     SGVVVFFADFGAFGVDETYNQERLNDLNHTNLKLVSTHCGLDVGEDGKTHQCIDYLGL
                     FNNLFGFKVIVPADPNQTDRAVRYMAGAWGNFLMTMGRSKVPVVTREDGTPYYDAAYR
                     FEYGQGDWIRRGRQATVVCMGAPMTAYAVAASDQLRAEGVEVGVLNLASPLQPDIAAL
                     REAAATGCLLTLEDHHIRTGLGAVVANFLAENRLAVKFQKLGIKGYGQSGTPEALYAK
                     EGIDTEGIKAAVKKLLAVR"
     gene            10550..11188
                     /locus_tag="PKN80_00045"
     CDS             10550..11188
                     /locus_tag="PKN80_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNQ34444.1"
                     /translation="MKIKICGLRQPRELDWARELGADLVGFNFYPASPRYLAAEALEK
                     MAAAGPGPLRAGVFVNPGWEELGALVRAAGLDLVQLHGDESPDFCRRVRSELGRPVIK
                     AFRLGGSLGPASLAAYLGAADYFLLDGGAAPGLYGGGGRSFPWDLAAPFKALGVPFFL
                     AGGLTPENVAAAIERVAPYGVDVASGVESAPGRKSRFKMEAFIRAARSAAGD"
     gene            11210..11974
                     /locus_tag="PKN80_00050"
     CDS             11210..11974
                     /locus_tag="PKN80_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNQ34445.1"
                     /translation="MTNQVALQLYTLRNELKTPEDISRTLAEVARIGYRAVQVSGVGP
                     IEPAAMKKILERENLTACSSHIPYQRLVGETEKVIEEARFLDQDSVACPQLPGELRTS
                     AAGFRQAARELSEAGKAFQQAGLVLLYHNHALEFERFEGRTGYQILVEESDPRFLQME
                     IDTYWVAFAGADPVDWCRKLAGRMHLVHFKDMGIQAGKQFMAEVGEGNLNWPAILTAC
                     RDGGVRWYAVEQDTCPRPPLESARISLENLKRMGLE"
     gene            12025..13038
                     /gene="purM"
                     /locus_tag="PKN80_00055"
     CDS             12025..13038
                     /gene="purM"
                     /locus_tag="PKN80_00055"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNQ34446.1"
                     /translation="MGMDYRKAGVDIAAAGSFVDSLKELAARTRVKGQLGDIGLFSGL
                     FSLQPLRYRQPVLVSGIDGVGTKILLAEAAGIYDTVGIDLVAMNVNDILTAGARPLFF
                     LDYLAVGRLDRKRELALIKGIVEGCRQAGCALIGGETAQMGDVYGPKGFDLAGCAVGV
                     VEKARIVSGAGIRPGDRLLALPSSGLHSNGFSLVRKLFSPKEIKRDPELARRLLTPTR
                     IYVKTILNLLEKVKVKGMAHITGGGPVGNIRRVLPDGTRAVLEAGTWEMPALFKEVQA
                     RGRVSRLELARTLNLGLGFVLVLSPAEAGRAVRVLRGLREPVLEIGRIEAAPGGPDCV
                     ISW"
     gene            13086..14468
                     /gene="argH"
                     /locus_tag="PKN80_00060"
     CDS             13086..14468
                     /gene="argH"
                     /locus_tag="PKN80_00060"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNQ34447.1"
                     /translation="MAKSRRLWGGRFKCKPAASAEAFGASLAVDRRLARFDVEGSLAQ
                     ALMLEKAGLVSAEEGRAIRAGLESIRAELSAGRFRFEPSDEDIHTAVERRLYELAGPA
                     AGKLHTGRSRNDQIALDERLYLRDLLARLDAALKELQKSLLAWARRYPRVIMPAFTHL
                     QHAQVILFAHWCLAYLEMFQRDRDRLAEAGRRVNISPLGAAAGAGSSLPLDPEFTAGQ
                     LGFAGVFANSLDAVSDRDCLVEVLADLALAAAHLSRLSEELVLWSTSEFGYLQLDPRY
                     CTGSSALPQKQNPDIPELVRGKTGAVFGALVSLLTTLKGLPLSYNRDLQEDKAPFFAA
                     ADSVLASISILGEMLPKSRLNPEAMERAAGSGYTTAIDLAEFLVTRGLPFREAHGLVG
                     GVVLYCQEQGKRLEELTLKELKSFSRLFNASALQALTPRESVRRRRSPGSTAPDQVAA
                     RLKYWAGKLA"
     gene            14546..16225
                     /locus_tag="PKN80_00065"
     CDS             14546..16225
                     /locus_tag="PKN80_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34448.1"
                     /translation="MKVKIRLKDPGNQALARPAAILARQLAERSGAEIISAGPETGSE
                     LAVALAIDPAAGREGYRIADGAPGEIIVAGGDRRGLIYGVGRLLHEAVYSPGEFRPGP
                     WRGASVPDKPVRGLYFATHFHNYYHDAPPAEIEKYLEELVLWGYNALVAWYDMNHYRG
                     LDDPDSRAMIERLRHLFSFARELGLDIGLVTVANESYRSSPRELGRVPEYAFMPGLLC
                     PNKPGGRELILKNMAGEFEAFAGLKLDFIWTWPYDPGSCFCEKCRPWGGNGFIEMSRE
                     IAGLARRYHPSARLVLSTWTFKPEDWEGLARAFRERPDWVDYIMADAHREFPEFPLKH
                     GVPGGLPLLNFPEISMIGMKPWGGFGATPTPERLQGFWPAAADRIAGGFPYSEGLHDD
                     INKVIISRFYWNRKTSALEALREYAHFEYGPGAVERVLEAVGIIEANHGRYWDVVPQA
                     PGFIRKREAPEVRDSGRAARLLEEVDRELPEARRRGWRWRLLLLRGRIERELRSNGGV
                     PNDACDEAFEELIAILHAERAEWRVAPLSRRLVARLDREKKQAEDFDGLGK"
     gene            16256..17320
                     /locus_tag="PKN80_00070"
     CDS             16256..17320
                     /locus_tag="PKN80_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ34449.1"
                     /translation="MNQLEIFRATVAHQPHSGFLYKVGFTNDLYRRLLERYRLPADAR
                     LDDYYECFRPAAVQPAPAGPVATPDFSRYFAGEAWPEGSLINKLGVLEIPAHFYHFTG
                     YVSPLRKAASLKELEEFPYPYAGAFDESGMAEKVRAAHEAGKAAMTGIVHLYEHAWQI
                     RGYEEFLQDMIERPEWVECILDRLAERNLKIAEVGARAGVDYLVTGDDIANQQNLMFS
                     PGMWWRFIGSRWARIYAAARRIKPDIQIWYHSDGNVYSLIPKLIEIGVTILNPLQPEC
                     LDVVRVKREFGRHLVFDGTVGTQSTFPFGTPEEVTRVVRERKRTLGCDGALILAPTHT
                     LEPEVTPENIEAFVAACREE"
     gene            17343..18467
                     /locus_tag="PKN80_00075"
     CDS             17343..18467
                     /locus_tag="PKN80_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ34450.1"
                     /translation="MPEIKPIRPKWKGTMSDRERFVSQLHYRPVDRSFNMEFGYWNEN
                     FKSWPIFVENGIKNNAEADLFFSFDRIETIGGRVWLSPPFPAGLVEETAATRIVQNSD
                     GLLAEVPRDGHDTIPHFIRASVRTPDDWRRCKAERFRLDDPARRVDVESLKRAHPAGR
                     DYPLGIHCGSMIGRIRDLLTMEGLVYAIYDYPEMVEEMVETACRLVEHFLDQVLGRID
                     FDFAAGWEDICSKSGPLVPVRFFREVVVPRYRRLGDRLRAAGVDIWYTDCDGDVRPLL
                     PYFLEGGINCIFPFEVNSSGHPAESLAKHAPELRIMGGVDKLQLRSGRPAIDACLERL
                     IPLVQAGGYIPFCDHRCPPDVNPDDYLYYLDRKEELFGMK"
     gene            18570..19796
                     /gene="lysA"
                     /locus_tag="PKN80_00080"
     CDS             18570..19796
                     /gene="lysA"
                     /locus_tag="PKN80_00080"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNQ34451.1"
                     /translation="MTENYLSWPEGRGRLEECFLDELADRYGTPLYLYSETGLRRRLG
                     RFRAAFKDLDPLVRYSCKANANTHLLSIIRAEGGGADVISAGELMLALAAGFEAADLI
                     MEGPGKRPDEMALALENGVAFFSVESEAELESLSRLAAERKLKATILLRLNPDIAAGG
                     HQYISTGRKENKFGLTEAEVISIFKAPERYRPLRIGGLHLHLGSQIDTPEPYLKAVRE
                     AKRLHASHPFEYLDLGGGFPVVYDKEVTELELFAEAIGRELDGLPVRVIFEPGRYLVA
                     PVGFLLTRITYRKDRPHRRFLVVDAGMNDLIRPSLYEAFHQIWPVRSSGAAPENFDVV
                     GPICESGDFLARERPLPADLAAGGLLLVGQAGAYGFAMSSNYNGRLRPAEVLVRGSES
                     RVIRRRQNYEDLLAGM"
     gene            20547..21731
                     /locus_tag="PKN80_00085"
     CDS             20547..21731
                     /locus_tag="PKN80_00085"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNQ34452.1"
                     /translation="MLADNESVYRARQKGLLRESVRTYRLVDLFAGAGGLTLGFTKTL
                     GQVFVPVWANDFNTYAARTYNVNFGDHCTTDDILEIVENRIDEIPKADVVIGGPPCQG
                     FSLLNKQREGDPRKQLWRPYFRIVERTGAEVFVMENVPQLLGSEEYEEIAEVAHSLGF
                     KMAFAKLCAADYGVPQTRWRAFIIGCKFAHPGKFFPPKKTHKRPADVNTGMLFSEEFD
                     QYVSRARPWRTVHDAIGDLPPPKGTEIRNEPPPLDLHFGRTPTPMSMKRYLSIPKEGM
                     NRFDLQKRAPELTPACWIRKTEGGTDLFGRLWWNRPAFTIRTEFFKPEKGRYLHPCQH
                     RPITHREAARLQSFPDSFKFIGSKIEIAKQIGNAVPPMLAARIADCVFALLATKEEGD
                     VG"
     gene            21721..22134
                     /locus_tag="PKN80_00090"
     CDS             21721..22134
                     /locus_tag="PKN80_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HNQ34453.1"
                     /translation="MSDKFPPEKRSQIMANVKGRNTSPERVVRGCLRRLRCRFRGNVK
                     ALPGKPDVVLTGRGKVIFVHGCFWHGHRRCRRSKRPTTNEMFWNKKIDGNIQRDKKVR
                     RALTRLGWKVLVVWECGTRNPTKLLAILERFVNAR"
     gene            22124..23056
                     /locus_tag="PKN80_00095"
     CDS             22124..23056
                     /locus_tag="PKN80_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNQ34454.1"
                     /translation="MRDRTRKETNSTPEAIRCQLEELISNFELHLRAGELRPKVLALV
                     SIFQGLRNLGKAIIPQKYASAARDRILYYFRKYPNVIINGDELLVVSGIQEYARRLRE
                     LRIQQGWAIASGVTIKDMCETESDEVPDDLKAMKPDDYVLLCDTQDRDAAYRWHLANT
                     IRKERSSVRDKILKFMRASVGQGVTNEELRYVAGDKTEWARRVRELRTEFGWPIVTKT
                     TGRPDLDIGVYVLQADRQSPEHDRTIPDDVRRHVMRRDGYKCTKCAWSHAEWNPSDPR
                     HLEIHHVKHHAKGGDNEEQNLTTLCIACHDAIHR"
     gene            23271..23753
                     /locus_tag="PKN80_00100"
     CDS             23271..23753
                     /locus_tag="PKN80_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34455.1"
                     /translation="MEGQAIKPTGCCDPFDPGPWQEKEITWREKLFVKDHVTSFLHVP
                     LNMGGKIVKNLGLIEKASARATRLMLTDEKSTWGSDIYIDVAREVPGAQMATISGTFL
                     TKVFEGPYRNAGQWAEEMKAYVKGQGKQLKKLYFSYTTCPKCARAYGKNYVVLFAQVD
                     "
     gene            23801..24115
                     /locus_tag="PKN80_00105"
     CDS             23801..24115
                     /locus_tag="PKN80_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ34456.1"
                     /translation="MSKSCRACGMPLIEKEDFAGGKEDALFCRHCVNADGSVKSGEEI
                     FEGGVRFFISQLGDDRKLAERITQKNMAGLPYWQGKNCRVLQGEMATDEEFAEVLKKL
                     GA"
     gene            24251..25366
                     /locus_tag="PKN80_00110"
     CDS             24251..25366
                     /locus_tag="PKN80_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ34457.1"
                     /translation="MKKVSIVSTSRGTAAGLQEALDRLGGLARFVTRGDRVMVKPNLN
                     GDCVTDLGLTEALIRLLLDLAPKEVFIAESTFGDAANTRFFFQKSGYAELAGRYGLRL
                     VNLNESEAVETPVARPLALEKLKLAREVFEADRLINLPKMKVHYATGVSLALKNLKGL
                     LVGDEKKHFHDVGLDRAIVDLNNTVPVHLNIVDAITGMERLGPHGGDLVRPDLLIAGA
                     AAWRTDCVGIRVMDYELKEVGHLARYLEVNGLDPADVELAGLGIEAARHPFKKVNMAN
                     LLPPNIRVRQRNACCACLNALILSGRFLERPPAAPVEVCLGSLPEASVAGCPQIAFGN
                     CAVKRCGPGLPKVEGCPPYPFELKKVLERNFKKAEGN"
     gene            25424..25921
                     /locus_tag="PKN80_00115"
     CDS             25424..25921
                     /locus_tag="PKN80_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34458.1"
                     /translation="MSILDNLREKHRVKLSESSRRKEISFSFREPDRVPIRISAAGSY
                     YAHMFGYNIKDYYQEADVQVDTQLRGIEWRLENLKDDNTDTEIHLDIGPISEGLVFDC
                     PIERPDGTSPRIVPILKDEHDFAELRVPDPKRLKGLEWLDRRFEDFYRAAARRESTCP
                     EAVPG"
     gene            25936..26565
                     /locus_tag="PKN80_00120"
     CDS             25936..26565
                     /locus_tag="PKN80_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34459.1"
                     /translation="MSAACAIMEPARVYLLMALKPKTVKELFAKMFDTFCALVDYYDE
                     RYGVRTETLGLANDNTCFISNAMYVCQVLSYDKALYERYGLKGRSMHTDGPSDHNFKT
                     FCEEFKLNVMDIGGWSSIDAAVAAMKGKVVIHGGLNCKDVYGSLTDAVKRKIRHAIRV
                     AGPGGAYEFAIGGETYVGVPAGTLIDLVNYVKTVGKYPLSGGTESTPGS"
     gene            complement(26679..26819)
                     /locus_tag="PKN80_00125"
     CDS             complement(26679..26819)
                     /locus_tag="PKN80_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34460.1"
                     /translation="MAGVPARGRPSTLSAAATLKVIKEELALKGVGMIEAAIAGTEQE
                     LL"
     gene            27019..28983
                     /locus_tag="PKN80_00130"
     CDS             27019..28983
                     /locus_tag="PKN80_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ34461.1"
                     /translation="MKLKTKLFLIFTSTSILIIFTFGTIIYKRLWNEQLALIQANLSQ
                     QLQNFDFSLKNFFAEVEGDVNALAQNEVVRSRDDSQFTSFLQANEKNFRYNYSELEKK
                     IIRIFNAYRLTHPYVNSVYMGRENGSMVRSHPWNRPIRYDPRQRPWYILGKKNPARVV
                     TTEAYPSLTTSDINLGFEKALLDEKGRVYGVVGIDVTLVNLTDYMVNFQVRPAGTIFL
                     VDRNGVIMASQAAGLQGRKIGEYAPALPSLLARDEREIAPIEIRGRRHFLFHRKFAEQ
                     DWRIVVLIPAANIEKMIGDHVLWMVLSLCLGLALLSLLTLSGLHLFVIHPLKKLTEGV
                     DFITRTGDLGRRIGIASRDELGTLASSFDQMVATLEESQRSLQVKEKVLTEYRDHLEE
                     LVRQRTRELEATLGELAVAKEKAEAADRLKSAFLATMSHELRTPLNSIIGFTGIILQE
                     LAGPLNEEQRKQLGMVRDSSRHLLALINDVLDISKIEAEQLEIRNEPFDLRASILKVA
                     GIVRPLAEKKQVDLRLEIAAEIGRFSSDSRRVEQVLLNLLNNAIKFTERGWVQLTAAL
                     AAEGNGTAVRISVADTGIGIKAEDLGKLFQPFRQIDSGLARQHEGTGLGLVICRRLAG
                     LLGGEIRVTSELGKGSVFTFILPVPSAETP"
     gene            28980..29351
                     /locus_tag="PKN80_00135"
     CDS             28980..29351
                     /locus_tag="PKN80_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ34462.1"
                     /translation="MKKRILLIEDNEQNRYLAAFLLEARGYEVLQAADGIRGIEMAGS
                     LRPELILLDIQLPGMDGYAVARELRANPALKTIPIVAVTSYAMAGDREKSLAAGCNGY
                     IEKPINPETFAAEVASYLEKD"
     gene            29601..30509
                     /locus_tag="PKN80_00140"
     CDS             29601..30509
                     /locus_tag="PKN80_00140"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNQ34463.1"
                     /translation="MFFYTATYTDERDRDFALSLGAERFVVKPTEPDSLMGIIEETLR
                     QSAAAVPKTPPRPAGKKSQEETVYLRQYNEAIIRKLEDKMEQLEQVNRKLEQNIAERK
                     RAEAGLQTSYEKLQKMIDETIEALGKMGEMRDPYTAGHQSRVARLAVTIARETGALEK
                     EMMVIGLLHDVGKIIVPVEILTKPGKLTEVEFQMIQPHSQAGYDILYGIEFPWPVALT
                     VLQHHERMNGSGYPNRLAENQIIMEARILAVADVVEAMASHRPYRLAVGTENALEEIS
                     GNRGVLYDPRVVDACLGVFRKGFKFD"
     gene            30603..31472
                     /locus_tag="PKN80_00145"
     CDS             30603..31472
                     /locus_tag="PKN80_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34464.1"
                     /translation="MPLNKILHLTSGDCAGGLLARSGLEGEIFVWHDILYDGPRRPGW
                     PDEAILAARARFIEAQTGRGLSREKILKSFRCQYARLRTEAGYGRIVLWFDACLFDQS
                     MLVHILVCLLELNRTENVELLCLDAFPGIVPFHGLGQLTAAQLLSVYGRRRPVTEAQF
                     LFAKRVDAAFALQDKQAFAELAAGVGAPLPWVPAAVARWLQEQPDGADGLGRLERLAL
                     EAVRNGKNRPREIFAAAAAGDTPPQFWGDTTLWAKINSLADKELVLIEGPSPRLPQWE
                     AGLDLDRFQITPA"
     gene            complement(31491..32012)
                     /locus_tag="PKN80_00150"
     CDS             complement(31491..32012)
                     /locus_tag="PKN80_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNQ34465.1"
                     /translation="MPFTPFHFGPGACLALPLKERLDLPVFLLSNLAIDLEPLAVISF
                     GLKYPLHGYAHTLLFGSLVGIGWAALAFAGRRHLRRLMNRLRLPYRPDFRIMAISALL
                     GAWFHVLLDAPLYSDIRPFYPLTANPLHGKISGGAVYLVCLLAFIPAAVLYLQAAGGK
                     RAENDRAGLVKKE"
     gene            complement(32125..33471)
                     /locus_tag="PKN80_00155"
     CDS             complement(32125..33471)
                     /locus_tag="PKN80_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNQ34466.1"
                     /translation="MAEWYSDYVQNPPRIVSPELPGPRSREIHGRAEKYMKGFSSQVQ
                     LFPVVFEEGRGPFLRDADGNTYIDFSSGIYVTGCGHAHPKIAEALKRMADRLWNAHDF
                     TASVKVEALELLVDVLPGDISGVQFYSDGTPAVEAGLRAARAITGRFEFLSFWKDFHG
                     KTLGSVSLAGMSPDKGIRSPGFFLAPRPNCYRCPFKMEHPACNLYCVDFTEKVLREET
                     TGRVAAVVMEPIQGWAGSVFPPEAFIPELKKRLEKLGILLFLDEILTGMGRTGDWFAC
                     DHYGVVPDIITLGKGLGNGFPVTVMAVREPYKEHLEKISASSSFGGNPLACAAALATL
                     KVIKEEKLLENARKIEAVIMKRLEAMKQKYRIVGDVRGRGCLFGVELVKDKQSKEPFE
                     AAGKLVYQKAFRKGLAWIPAGHILRMSPPLVINEELALKGVGMIEEAIAETERELL"
     gene            complement(33464..>34210)
                     /locus_tag="PKN80_00160"
     CDS             complement(33464..>34210)
                     /locus_tag="PKN80_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNQ34467.1"
                     /translation="LSAGTPVFAGGHDQYCAALGAGAAAAGTALVSCGTAWVILITTR
                     RPVYDPARRLAPGPHLLPGRFGLMGAASNGGILYDWFSRNLKQTRPAEGETPSPVLVL
                     PNFIRGRGGLLNLGLADGPAEILRAVMETLGLQIREYLDLAAPRLGGDRVRKLVAIGG
                     PVRQTSLPRLLADITGLEVTVPEISEAAGRGAARLALAGLSGRHPESRFKLAGRRFRP
                     HPGRTEAYVKKYELYRRLKRQLEKEGENDG"
BASE COUNT         6408 a        10661 c        11037 g         6104 t
ORIGIN      
        1 ctgctggcgg gatggatcgg cctctacaac atgttcatcc ttttgaagaa caacgtggcg
       61 cgggcctggt ccaacatcga cgtccttctg aaacagcgtt atgacgagtt gccgaaactg
      121 gtcgaagtct gccggtctta catggagtac gaaaaggaga ccctggagcg ggtgatccgg
      181 ttgcgctcgg tcatcggcgg cgccagcggc ctcgaggaga ggctcaaggc cgagggtgaa
      241 ctggccgggg cgctcaagac gctctttgcg gtctccgaga attatccgca attgaaggcc
      301 aacgaaaatt tcgagcagct gcgccagcgg atttcaggcc tggagaacga aatcgccgac
      361 cggcgcgaat tttacaatga atcggcaaat ttgtataata taaaaatagc ccagattccg
      421 gatgtcctgg tggcccggat gatgggttgc aagccgattc cgctcttcga gaccgccccg
      481 gagcagcggg agagttcgcc cttgaatatc tgacacgcac cattaatctg gagactccga
      541 tgtggaagag ccgccttttt accccgggcc cgacggccgt tccggacgag gttctgctgg
      601 agatggcccg cccccttatt caccaccgga ccccgcagtt ccggaaactg ctcaaggagg
      661 ccttcgaggg tttgaaatac gtctttcaga ccgggcagga cgtcatgatc ctgacttcat
      721 ccggcaccgg ggccatggag gcggcggtgg ccaacctcct ctcgcccggg gatcactgcc
      781 tggtggttga aggcggcaag ttcggcgagc gtttccgaga actggcccag gcttacggcg
      841 cggcggtcaa aacgattccg gtcgaatggg gcaggtcggt gtcagccggc gcgatcgagg
      901 ccgccctcaa gtccgacccg gccgtgaagg ttgtcttcac cacgctctgc gagaccagca
      961 ccggcgccca cctcgatctc aaggccatcg gcgccgcggt ccgccagcac ccggagaccc
     1021 tgctggtggt ggacgccatc agcagcctgg gcgcggtgga gtgccggatg gacgagtggg
     1081 gtgttgacgc gctgatctcg gcctcccaga aggccttcat gctgccgccg ggactggcct
     1141 tcatcgcgtt gagcccgaag gcctggaagg cggtcgaagc cgcccgttcg cctcgttact
     1201 actttgacct ctccgcctac cggcagacgc tggccaagga ggatactccc tacaccccgg
     1261 ccctgaccct ggtgatcggg ctggtccggg tgctgcggat gatgaaggag gaggggctcg
     1321 agaacgtctg gcgccgccac cggattctgg ccgaggccac tcgggaggcg atgcgcgccc
     1381 tggggttgaa actgctggcc gagcatccga gcgacggcct gacggccgtg ctgctgcccg
     1441 agggactgga tgccgaggcg atggtcaagg ccttgcgcga ccagtcgggc atcaccttcg
     1501 ccggcggcca gggaaaactg aaggggcgca tcttccggat cgcgcacatg ggctgggtgg
     1561 atgggttcga ggtcctcgag gcgattgccg cggtcgagat ccagctggcc cggctcgggt
     1621 atggtttgac cccgggcgcc ggcgtggccg cggccgaaaa agtactcctt tcggccggtg
     1681 gctgaaatcg ctccgggtgt accataatcc gaagtgaaag gacatcagtg gaagcgaaac
     1741 aaatcagagt cctggtaacc gatccccttt cgaaagaggg ggtggcgata ctgaagaatg
     1801 agggttttga agtcgaggag accggcaaac ttactccggc cgagctggcc gggaaggtaa
     1861 agggttttga cgccctcgtc gtgcgcagcg gcacccgggt caaccgggag gcgattgccg
     1921 cggccgataa cctcaaggtg atcggccggg cgggcgtagg tctggacaat gttgacctgg
     1981 agagcgcgac cgccaagggc atcatcgtca tgaactcgcc cgaggggaat accatctcga
     2041 ccgccgagca cgctttcgcg ctgattctct ccatgatgcg caatatcccc gaggcccacc
     2101 attcggtacg ggaggggcgt tgggagaaga agggattcac cggtaccgag ctctaccgga
     2161 aggtgctggg catcatcggt ttcggccgga tcggccagcg ggtggcccag tacggccggg
     2221 tcttcggcat ggaagtgctg atttacgacc cctttgtgag tgaagagaag gccctccagg
     2281 ccggcgtcaa cctggtggac caggaacacc tgcttaaaaa cgccgacgtc atcaccctgc
     2341 acctgccgct cagcgaggag acgcgcgacc tgatcggggc ccgggaactg gctctgatga
     2401 agaagggcgc cttcctggtc aactgcgcgc gcggcggact ggtcaacgag ccggcgctca
     2461 tcgaggccct gaaatcggga cacctcaagg ccgccgccct ggacgtcttc agcgaggagc
     2521 cgcccgccga tccggccttc cgggagacgc cgcgtctggt gctcaccccc cacctgggcg
     2581 cctcgaccga ggaggcccag gtcaacgtgg cccgggatat ctgccgccag atcgttgact
     2641 atttccggaa gggcatcatc agcaacgcgg ccaatctgcc gaccatcagc gagaaggtgc
     2701 tcgagtatct ccggccttat ctcaacctgt gcgaaaaact gggtcttttc ctggcccaga
     2761 ccgcgcccgg ccggatcaac gaggtcaagc tcgagtatta cggcgacatg gcctcttttg
     2821 acctctcgcc cctgcaccac agtttcctga agggtctgct ctcccagatg ggggccgggg
     2881 tcaactacat caacgcgccg gtcatggccc aggagcgggg cattcagttc gcggagcaga
     2941 agagcaccat ctcccaggaa tttgccaacc tgatcaccgc caccgtctcc gacagcggct
     3001 cctcgatttc ggtctccggc accctcttcg gcaagactga gccgcggctg gtcaagatcg
     3061 acggttaccg gtgcgaagcg gtgccggagg gttacctgct cttctgccgg aacgaggaca
     3121 agccggggat cgtcggccag atcggcaccc tgctggcgtc gcgccagatc aacatcgccg
     3181 gcatgacgct ggggagaaag gaacggggcg gcctggccat cacgatcctg aatgtcgacg
     3241 aaatggtgga cgagccgctc cgggaacgga tccggcagat caaggaaatc aaaagcgttt
     3301 cacttatcaa gctctgactc aacggaatcg ttaacgaggt cgcgggcagg aaatgaaatc
     3361 aaacaaaggc gcgaaatata accaagagat atcaatggtt cgtatttttc cgggtttgac
     3421 cagggatcct ttccgatggg ccttcgggcc gcgccgcggc cgcgggcgcc gtcggaacgg
     3481 cggcgtcgag ccgtcgccag gttccacccg gccttagtac gccgccttgt aaagatttct
     3541 cccgccgcgc ggcctccgaa gaggaggtgg cgccatggaa attctactgg gtttgctggg
     3601 cctcgggctt ggctacctga ttcacctgtc gatcacttcc cgcaacctta aggcgtccga
     3661 agcgcgcagc caggggctgc tcaaggaggc cgagcgggag atcagccagc gccggaagga
     3721 actggagctg gaggtcaagg aaagcctgat caagcagcgg ggcgatttcg agcgcgagac
     3781 gcgcgaggaa cgccgccagc ttcaggtcct ggagcagcgg ctcaaccagc gcgagagcaa
     3841 cctggaccgg aagctgaacc tggtcgacaa gaaggagacc gacctcaacc agcgcgaaaa
     3901 gaccctcgcc gaccaggaga agaaggtggc cgatcggcag accgagctcg acggcgtgct
     3961 ggaagaggag aagcggcgcc tttcgcaggt ggccgggctt tcccaggatg aggcccgccg
     4021 gattctcatc gcccggatgg aggacgacgc ccgccacgag gccgccctgc gcatcaagcg
     4081 catcgaggag gagacccgcg aggaggccaa caacatttcc cggaccattc tcctggaggc
     4141 catccagcgc tgcgcctccg aacaggccga gtcctcgacc acctcggtgg tcagcctgcc
     4201 ctcggacgag atgaaggggc ggctgatcgg ccgcgagggg cgcaacatcc gggccttcga
     4261 ggcggccacc ggggtcgacc tcatcgttga cgacacgccc gaggcggtca ccatctccgc
     4321 cttcaacatc taccgccggg aaattgcccg gctggtcctg aagcgcctca tttccgacgg
     4381 ccgcatccat cccggccgga tcgaggaagt ggtcgagaag gtcaaggccg agatggagga
     4441 ggagaccctg aaggtgggca aggaagcggc cctctccctg ggcatccaca agatcaatcc
     4501 ggaactgctc cggatgctgg gccggctcaa gtaccgcacc tcttacggcc agaatgtcct
     4561 ggaacactcg atcgaggttg cccacctggc cgggactatg gccggcgagc taaagctgga
     4621 ccagaccatg gccaagcggg ccggccttct tcacgatatc ggcaagagcg tcgaccagga
     4681 gatggagggg gaccacctgg agatcggcta cgagatcgcc agccgctacg acgagtccaa
     4741 ggaggtcctc gaggccatcc gcggccacca cgacgatttt cggccgaccg ctccttacgc
     4801 ggtgctgatc tcggccgccg acgccctttc ggccagccgg cccggcgcgc gccgcgaaac
     4861 gctcgagggt tacatcaagc ggctggagaa gctggaggcg atcgccaccg gcttccgcgg
     4921 cgtcaaccag gcctacgcca tttcggccgg ccgcgaggtg cgggtgatcg tcaagccgga
     4981 ggcgatgagc gacgatgagg cctcgctgct ggcccgccag atcagcggca agattgagga
     5041 ggggctggag tatcccggca cgatcaaggt gaccgtgatc cgggaggcgc gctactcgga
     5101 gatggccaag taatggggag cagcatgaaa agttaccggg aactctggcg ggacatcatg
     5161 ttctaccggg ggcacgatcg gatgccggtc gtccactggc ggggctggcc cgagacccgg
     5221 gagcgctggc tcaaggaagg cctgcccccg gatgtcgagg agatggagta tttccgggcg
     5281 gtgccgaccg gccactcggt ctcggtcgaa ctggggctgc ggccggggtt caccgaagag
     5341 accatcgagg agaccgccga gtaccgcgtc ttccgcgatg ccagcggcgt ggttcagaag
     5401 gcctggaagc atcagagcaa cattccccat tacacggatt tcaccttcaa gaccgcccgg
     5461 gactggcccg agtacaagaa gcggctccag cccggaccgg agcggattcc ggccgacctg
     5521 gacgaaaagc tgcgggcggc cgaggtccgg gactgcccga tcatcatccg gatctgctcc
     5581 ctgatgggct gggtccgcaa ctggatgggc gtggagaatt tcgcctacct gatgtacgat
     5641 gatcccgacg tttatgccga cgtcgtcaac accctggccg acctctcctg ctggggaatc
     5701 gaccaggtcg tcccccgcct gaaggtcaag ccggacctgg ccttcggctg ggaggacatc
     5761 agcggcaaga gcggcccctt cgtcagcccg tcggtcttca agcgctgcgt ggcgcccggc
     5821 taccggaaga tccgcgacaa gctcgagagt cacggcatcc acctgatggc cgtcgacagc
     5881 gacggcgacg gccgggcgct gatcggcccc tggctggagg ccggcgtcaa catccagttt
     5941 ccggtcgaga tcggcacctg gcagaccgac ccgatggagc tccggcgccg gtacggacgc
     6001 gaactgcgca tcatcggcgg ctacaacaag ctggccctcg agaaggggcg ggcggccatc
     6061 gatgctgagc tggaacggcg cttgcccctg atgaaggagg gcggttttct cctggcgccc
     6121 gaccatctca tcactccggg cgtgccgctg gccgattacc tttactacct ggaccgggtt
     6181 cggcagctcc gattctgagg cggtccctgg agacatggag tacatcgccg gggttgacat
     6241 cggcggcacc aagattgccg cgacgctctt ccggccggat ggggaactcc tggccaaggt
     6301 ggtcttcccg acccgggtcg aacgcggtcc ggaggcggcc ctggccgaaa tccggacccg
     6361 cctggaagag ctccgccgcg gccggccggc cgaacggctg ctggggatcg gcatctcctg
     6421 cggcggtccg ctcgacagcc ggaccgggct catcctctcg cctcccaacc tgcccggctg
     6481 ggcctcggtt cccgtcaccc gcttcctgtc cgaggccttc cgcgtgccgg cccgcctcga
     6541 gaatgacgcc aacgccggcg ctctggccga gttccgcttc ggagccggcc gggcaagcgc
     6601 cgcttccggc cggccggtcc gcaacctggt cttcatgacc atgggcaccg gcatcggcgc
     6661 cggcctgatt ctcgacggcc gcctctaccg gggagcgacc ggcctcgccg gcgaaatcgg
     6721 ccacaccaag attatcccgg gcggcccgga atgcggctgc ggcggccgcg gctgcctgga
     6781 ggccctggcc gccgggccgg ccatcgcgcg ccgcgcccgg gaacggcgga aggagttccc
     6841 ggcgagccgg atttggacgc tggccggcgg ccggccggag gcggtcaccg ccgccgaggt
     6901 cttcgcggcg gccgccgccg gggaccggct gggccgcctg gtggttgagg agacggccta
     6961 ctacctgggc ctgggtctgg ccaacctggt gaacctgctc aacccggaag tgatcgtgct
     7021 gggcaccatc gccacccgtt acggcgattt cttcctggaa ccggtgcgcc gggtggtggc
     7081 cgccgaggcc tggccggatc cggtggccgc ggtccggatt accggttcgc cgctgggcga
     7141 cctgaccggg gattacgccg ctctggccgt tttcctggat cagtcgccgg gggcctgagc
     7201 cggtgggccg ccggggcggc gccggccgct ggccgggcct ctttttattt gcgctctttc
     7261 ccggaactaa agtataataa atacctgaaa atctgcggtt tgcacgaccc ccttccgcac
     7321 ctaaatcttc accaccacac aggaggcatg atggcgggaa acctgatcct ggcccagtcg
     7381 ggcggaccga ccgcggtcat caacgggagc attgccggcg tggtcatgga agcgctgcgc
     7441 cacaaggaaa tcggcgggat ttacggcgcc gtcaacggca tcgagggcgt cctggatcag
     7501 aacctggtcg acctgcgcgc cgaggagccc gaggttatca gcgggctgcg ttccactccg
     7561 tcggccgcgc ttggcagctg ccgccgcaag ctcaaggcgg acgattacca gaagatcctg
     7621 gacgtgctga agaagtacga ggtgcgctac tttctcatcg ccggcggcaa cgattccatg
     7681 gacaccgccc tccagatgca caagctggcc aggtcgagcg gctacgagct cttctcggtg
     7741 ggcgtgccca agacggtcga caacgacctg cgggtaaccg accactgccc cggctacggc
     7801 agcgtggccc gctactgggc gatctcgggc cgggacgccg gccgggacac cgacgccatc
     7861 tatcacccgg acacggtcaa gatcctggag accatggggc gggataccgg ctggatcacc
     7921 gcggccgccg gcctggcctg cgaggccggg gacgacgccc cgcaactcgt ttatgttccg
     7981 gagatcccct tcgaccagga gcagttcctg ggcgacgtgg accgggtcta ccggaagctc
     8041 ggccgggtgg tgatcgccgt ctgcgaggga ctcaaggaca agagcggcga attcgtgacc
     8101 gcctccggcc gcaagacgga caccgactcc ttcggccaca agcagctggg cggtgtcggt
     8161 gacgtgctct gcgatctgat cgccgaaaag ctgggcctca agtcccgctt cgacaagccg
     8221 ggcaccatcc agcgcatggc catggtctgc gcctcggagg tcgaccggga agaggcctac
     8281 caggtcggca agcaggcggt ccggctggcc ctggccggcc gcagcggcgt gatggtcacg
     8341 atcgagcgcc agccgggtgc cgagtaccgg gtggcctacg ggaccgggga cctgacggag
     8401 atcgccaacc tgcgcaagga cctgccgcgg gagttcatga acgccgccgg caaccatgtt
     8461 tcccaggcct tcctggatta cgcccggccc ctggccggcg ggccgctgcc gccctacgtg
     8521 cggttgaaga aggtggcggt tcccaagcgg tgaaacggca tgcaaaccaa aatcgaaagg
     8581 atggtccgat gatcaaggaa agatttgccg cggaacgcct ctccgacgaa cagctccggg
     8641 aactggccga actctcccgg gtctgcaagg gggacattct gaagatgact accctggccg
     8701 cctgcggcca tccgggcggt tccatctcct cgctcgacct ctacctggtt ctctttaagt
     8761 acgcggcggt cgatcccggc gacccggaca acccgaaccg cgaccacatc ctgatcagcc
     8821 acggtcacac ctcgcccggg gcttacgcaa cgctgggccg gctgggcttc ttctccattg
     8881 aggcggccgt gagcggcttc cgcaagatcg acaccccctt cgagggacac gtggtccgcg
     8941 aactgcccgg cctggactgg tccagcggca acctgggtca gggcctctcg gccggttgcg
     9001 gtttcgcgct ggcctcgcgg atcctgaagc gcggcttcca cgtctttgtg gccatgagcg
     9061 acggcgagca tgccaagggg cagatcgccg agtcccggcg cttcgccgcc aagtacggtc
     9121 tggacaacct taccgtggtc atcgactaca accacatcca gatcagcggc aagatcgagg
     9181 atgtcatgcc ccagaatatc aagggcaatt acctggccga cggctggcgg gtcctggagg
     9241 ttgacggcca cgaccaccag gctatctacc aggcctgccg ggaagcggcc tccgacggga
     9301 agccgacggt catcctggcc gagacggtca tgtccagggg catctccttc atggaaggcc
     9361 tgcccaagta ccacggcgcg gccctcaagc cggacgactg ccggaaggcc ctggccgaac
     9421 tcggcctgga ggacgacctg gaccggctgg gacagctgcg caagaactgg tccttccagg
     9481 tgccggtccg ccgtttcgag tatccggtaa gcctcaacct cggcgagccg gttctttacc
     9541 cggccggctc cggtgccgac aaccggggcg ccttcgggaa ggccctgctg tcgatcgctt
     9601 cggccagctg cggttcggat gacaaaaccc cgctggtggt ggtggattgc gacctggcca
     9661 cctcggtccg gaccgaggat ttcgccaagc tggccccctg ttccttctac gaggccggcg
     9721 tccaggagca taccgcggcc accctttccg gggccctctc ggccagcggc gtggtcgtct
     9781 tcttcgccga tttcggcgcc ttcggggttg acgaaactta caaccaggag cgtctcaacg
     9841 acctcaacca caccaacctg aaactggtct ccacccactg cggacttgac gtgggcgagg
     9901 acggcaagac ccaccagtgc atcgattacc tgggactctt caacaatctc ttcggcttca
     9961 aggtgatcgt gccggccgat cccaaccaga ccgaccgggc cgtccggtat atggccggcg
    10021 cctggggaaa cttcctgatg accatgggcc gttcgaaggt gccggtggtg acccgtgagg
    10081 acgggactcc ctattacgac gccgcctacc ggttcgagta cggccagggt gactggatca
    10141 ggcgcggccg gcaggcgacc gtcgtctgca tgggcgcccc gatgaccgcc tacgcggtgg
    10201 ccgccagcga ccagctgcgg gcggaaggcg tcgaggtggg cgtcctcaac ctggcctccc
    10261 cgcttcaacc cgacatcgcg gcgctccggg aggcggccgc gaccggctgc ctgctgacgc
    10321 tggaggacca ccacatccgg accgggctgg gcgcggttgt ggccaatttc ctggccgaaa
    10381 accggctggc ggtgaagttc caaaaactgg gcatcaaggg ttacgggcag agcggcacgc
    10441 ccgaggcgct ctacgccaag gaagggatcg ataccgaggg gatcaaggcg gcggtcaaaa
    10501 agctgctggc cgtccgctga gcggccggcc gccggttcga aaaggccgga tgaagatcaa
    10561 gatctgcggc ctgcgccagc cccgggagct ggactgggcc cgggaattgg gcgccgacct
    10621 ggtcggcttc aacttttacc cggccagtcc ccgttatctc gcggccgaag cactggagaa
    10681 gatggcggcc gccggaccgg gtccgcttcg ggccggggtc tttgtcaatc cgggctggga
    10741 ggaactgggc gccctcgtcc gtgccgccgg gctggacctg gtccagctgc acggggatga
    10801 gtccccggac ttctgccgcc gggtccgttc cgaactgggc cggccggtta tcaaagcctt
    10861 ccggctcggc gggtccctgg ggccggcttc ccttgccgcc tacctggggg cggccgatta
    10921 ctttctgctt gacggggggg ccgcgcccgg cctctacggc ggcggcggcc gttcctttcc
    10981 ctgggacctg gcggcgccct tcaaggcgct gggggttccg tttttcctgg ccggaggctt
    11041 gacgccggag aatgtcgccg cggcgattga aagggttgcc ccctacgggg tcgacgtcgc
    11101 cagcggggtg gagtccgcgc ccggccggaa gtcccgtttc aagatggagg ccttcatccg
    11161 ggcggcccgt tcggccgccg gcgactgact ggaacaagga gatgacgcga tgaccaacca
    11221 ggtggccctt cagctttaca cgctccggaa tgaactcaag accccggagg atatcagccg
    11281 caccctggcc gaggtggccc ggatcgggta ccgcgccgtc caggtttccg gggtcggccc
    11341 gatcgaaccg gcggcaatga agaagatcct ggaacgggag aacctgaccg cctgttccag
    11401 ccatatcccg taccagcggc tggtcggcga aacggagaag gtcatcgagg aggcccgctt
    11461 cctggaccag gattcggtgg cctgcccgca gctgccgggc gagttgagaa cctccgccgc
    11521 cggcttccgg caggcggccc gggaactttc ggaggccggg aaggcctttc agcaggccgg
    11581 cctggtcctg ctttaccaca accacgccct ggagttcgag cgcttcgaag gacgcaccgg
    11641 ctaccagatc ctggtcgagg agagcgaccc ccgcttcctg cagatggaga tcgacaccta
    11701 ctgggtcgcc ttcgccggcg ccgacccggt tgactggtgc cggaagctgg ccggccgcat
    11761 gcacctggtg cacttcaagg acatgggcat ccaggccggc aagcaattca tggccgaggt
    11821 gggcgaaggt aacctcaact ggccggccat cctgaccgcc tgccgcgacg gcggcgtccg
    11881 ctggtacgcg gtggaacagg acacctgccc gcggccgccc ctggagagcg cccgcatcag
    11941 tctcgagaat ctcaagcgga tggggctgga gtgaccggaa gccggttggc cggaacggcc
    12001 cggaagcgaa ggggagccaa ggcgatgggc atggattacc ggaaggccgg cgtcgacatc
    12061 gcggcggccg gcagttttgt cgattccctc aaggaactgg ccgcccggac ccgggtcaag
    12121 ggccagctgg gcgatatcgg tctcttctcc ggtctctttt ccctgcagcc gctccgctac
    12181 cggcagccgg tgctggtctc cggcattgac ggggtcggga ccaagatcct cctggccgag
    12241 gcggccggga tctatgacac ggtcggcatc gacctggtgg ccatgaatgt taatgatatc
    12301 ctgaccgccg gggcccggcc gctcttcttc ctggactacc tggccgtggg acgcctcgac
    12361 cggaaacggg aactggccct gatcaagggc atcgtcgaag gatgccgcca ggccggctgc
    12421 gccctgatcg ggggcgagac cgcccagatg ggagatgttt acgggccgaa gggtttcgac
    12481 ctggccggct gcgcggtcgg cgtggtggag aaggcccgga tcgtctccgg cgccgggatc
    12541 cggcccgggg accggttgct ggccctgccc agcagcggcc ttcacagcaa cggcttttcg
    12601 ctggtccgaa aactattcag cccgaaggaa atcaagcgcg atccggaatt ggcccgccgc
    12661 ctgctcaccc ccacccgcat ctacgtaaag acgattctca atcttctgga aaaggtgaag
    12721 gtgaaaggca tggcccacat caccggcggc ggcccggtcg gcaacatccg gcgggtgctg
    12781 ccggacggta cccgggcggt cctggaggcc gggacctggg agatgccggc gctcttcaag
    12841 gaagtccagg cccggggccg ggtcagccgg ttggaattgg cccgcaccct gaacctcggc
    12901 ctcggtttcg tgctggtcct gtcaccggcc gaggccggcc gggcggtccg ggttttgcgc
    12961 gggctccggg agccggtcct ggagatcggc cggatcgagg cggcgccggg cggccccgac
    13021 tgcgtaatat cctggtaatc cgtttttgcg cccgttttat atttgggtta tacttttaac
    13081 tgtaaatggc caagagcagg cgtctttggg gtggacgttt caaatgcaaa ccggccgcca
    13141 gcgccgaagc cttcggcgct tcgctggcgg ttgaccgccg gctggcccgc ttcgacgtcg
    13201 aggggagcct cgcccaggcg ctgatgctcg agaaggccgg cctcgtctcc gccgaggaag
    13261 ggcgggccat ccgcgccggg ctggagtcga tccgggccga gttgtccgcc ggccgttttc
    13321 gtttcgagcc ctccgacgag gatatccata ccgcggtcga acgccgcctc tacgaactgg
    13381 ccggtccggc cgccggcaag ctgcacaccg gccgcagccg caacgaccag atcgccctcg
    13441 acgagcgtct ttacctgcgc gacctgctgg cccggctgga cgccgccctc aaggagcttc
    13501 agaagagcct gctggcctgg gcgcgccggt atccccgtgt catcatgccc gccttcaccc
    13561 accttcagca tgcccaggtc atccttttcg cccactggtg cctggcctac ctggagatgt
    13621 ttcagcgtga ccgggaccgg ctggccgagg ccggccgccg ggtcaacatt tcaccgctgg
    13681 gcgccgccgc cggcgccggt tcttctctgc cgctggaccc cgaatttacg gccggccagc
    13741 tgggttttgc cggggtcttc gccaacagcc tggacgcggt cagcgaccgg gactgcctgg
    13801 tggaggtgct ggccgacctg gccctggccg cggcgcacct ctcccgcctg agcgaggagc
    13861 tggtactctg gtccaccagc gagttcggat acctgcagct cgatccgcgc tactgcaccg
    13921 gcagcagcgc cctgccccag aagcagaatc ccgacatccc ggaactggtg cggggcaaga
    13981 ccggcgccgt ctttggcgcc ctggtcagcc tgctcaccac cctcaagggc ctgccgcttt
    14041 cctataaccg cgacctccag gaggacaagg cgcccttctt cgcggccgcc gattccgtgc
    14101 tggcctccat tagcatcctg ggcgaaatgc ttcccaagag ccggctcaac ccggaggcca
    14161 tggagcgggc ggccggttcc ggctacacaa cggccatcga cctggccgag ttcctggtca
    14221 cccggggcct gcctttccgc gaggcgcacg gcctggtggg cggggtggtg ctgtactgcc
    14281 aggagcaggg caagcggctg gaagagctga ccctcaagga actgaaatcc ttctcccggc
    14341 tcttcaacgc ttcggcgctc caggcgctga cgccgcgcga atcggtgcgc cgccgccgat
    14401 cgccgggctc gaccgctccg gatcaggtgg ccgcccgcct gaagtactgg gctggaaaac
    14461 tcgcctgacc ctcccccccg ctttaaattt tacttatcct ttgcctgttt tctttaaccc
    14521 ggcggtttcg aaaggatcgg ttttgatgaa agtaaaaatc aggctgaaag atcccggcaa
    14581 tcaggccctg gcccgtccgg ccgccatact ggcccgccag ctggccgagc gctccggtgc
    14641 ggaaataatt tcggccggcc cggaaaccgg ttcggaactt gcggtggcgc tggcgattga
    14701 cccggccgcc ggccgcgagg ggtaccggat cgccgacggc gccccgggcg aaataatcgt
    14761 cgccggcggc gaccggcgcg gcctgattta cggggtcggc cgcctccttc acgaggccgt
    14821 ttactccccg ggtgaattcc ggcccggccc ctggcggggc gcctcggtcc cggataagcc
    14881 ggtgcgcggc ctctacttcg ccacccattt tcacaactac taccacgacg cgcccccggc
    14941 cgagatcgaa aagtatctcg aggagctcgt cctctggggt tacaacgccc tggtggcctg
    15001 gtacgacatg aaccactacc ggggcctgga cgacccggac agccgggcga tgatcgagcg
    15061 cctgcgccac ctgttttcct tcgcgcgcga gctgggcctg gatatcgggc tggtgacggt
    15121 tgccaacgag tcctaccgga gcagcccgcg ggaactgggc cgggttccgg agtatgcttt
    15181 catgccggga ctgctctgtc cgaataagcc cggcggccgg gaactgatcc tgaagaacat
    15241 ggccggcgaa tttgaggcct ttgccggttt gaagcttgat ttcatctgga cctggcccta
    15301 cgatcccggc agctgcttct gcgaaaaatg ccgcccctgg ggcgggaacg gcttcatcga
    15361 aatgagccgg gagatcgccg ggctcgcccg ccgttaccac ccgtcggccc ggctggtcct
    15421 ctccacctgg accttcaagc cggaggattg ggaagggctg gcccgggcct tccgggagcg
    15481 gcccgactgg gtcgattaca tcatggccga tgcccaccgg gagttccccg aatttcccct
    15541 gaagcacggg gtgcccggcg gcctgccgct cctcaacttt ccggagatca gcatgatcgg
    15601 catgaagccc tggggtggct tcggcgccac gccgacgccg gaacggctgc agggtttctg
    15661 gccggcggcg gccgaccgga tcgccggcgg cttcccctac tcggaggggc tgcacgacga
    15721 catcaacaag gtcatcatca gccgcttcta ctggaaccgg aagacctctg ccctcgaggc
    15781 gctccgggag tacgcgcatt ttgaatacgg ccccggggcg gtcgaacggg tgctggaggc
    15841 ggtcgggatc atcgaggcca atcatggccg ctactgggac gtcgttcccc aggcgcccgg
    15901 gttcatccgc aagagagagg cgcccgaggt gcgcgattcc ggccgcgccg cccggctgct
    15961 ggaagaggtt gaccgggaac ttcccgaggc gcggcgccgg ggctggcgct ggcggctgct
    16021 gcttctgcgc ggccggatcg agcgggagtt gcgaagtaac ggcggcgtgc ccaacgacgc
    16081 ctgcgacgag gccttcgagg agttgatcgc cattctgcac gccgaacggg ccgagtggcg
    16141 ggtggcgccg ctgagccggc gcctggtggc ccgcctggac cgcgagaaga aacaggccga
    16201 ggatttcgac ggcctgggta agtaaagtaa accccggata aaagggagaa ccgcgatgaa
    16261 ccagctggaa atcttccggg cgacggttgc gcaccagccg cactcgggat ttctctacaa
    16321 ggtcggtttc accaatgacc tctaccggcg cctgctggag cgttaccggc ttccggccga
    16381 cgccaggctt gacgactact acgagtgctt ccggccggcg gcggtccagc cggcgccggc
    16441 cgggccggtg gcgacgccgg acttttcccg ctacttcgcc ggcgaggcgt ggccggaggg
    16501 ctcgctgatc aacaagctgg gcgtgctgga gatcccggct catttctacc acttcaccgg
    16561 ctacgtcagc ccgctgcgga aggccgcctc cctgaaggag ctggaggagt ttccctatcc
    16621 ctacgccggc gctttcgacg agagcgggat ggccgaaaag gtccgggcgg cgcacgaagc
    16681 gggcaaggcg gccatgaccg gcatcgtcca cctctacgag catgcctggc agatccgtgg
    16741 atacgaggag tttctccagg acatgatcga gcggccggag tgggtcgagt gcatcctgga
    16801 ccggctggcc gagcgcaatc tgaaaatcgc cgaggtcggc gcccgggccg gcgtcgatta
    16861 cctggtgacc ggcgacgaca tcgccaacca gcagaacctg atgttctccc ccgggatgtg
    16921 gtggcgcttc atcggctccc gctgggcccg gatttacgcg gccgcccgcc ggatcaagcc
    16981 ggacatccag atctggtacc acagcgacgg caacgtttac tccctgatcc caaaactgat
    17041 cgagatcggc gtcaccatcc tcaacccgct ccagcccgag tgcctggacg tggtccgggt
    17101 caagcgtgag ttcggccgcc acctggtctt cgacgggacc gtcggtaccc agtccacctt
    17161 tcccttcggc acccccgaag aggtgacgcg ggtggtccgg gagcggaaac ggacgctcgg
    17221 ctgcgacggc gcgctcatcc tggcgccgac ccataccctg gagccggagg ttacgcccga
    17281 gaacatcgag gccttcgtgg ccgcctgccg cgaggaataa ccttgaaccg gagaggcctg
    17341 tcatgccgga gataaaaccg atccgcccca agtggaaggg gaccatgagc gaccgggagc
    17401 gcttcgtcag ccagctccat taccggccgg tggaccgctc cttcaacatg gagttcggct
    17461 actggaacga gaacttcaaa tcctggccga tcttcgtgga gaatggaatc aagaacaacg
    17521 ccgaggcgga ccttttcttc agcttcgacc ggatcgagac cattgggggc cgcgtctggc
    17581 tctcgcctcc ctttccggcc ggcctggtcg aggagaccgc cgccacccgg atcgtccaga
    17641 actccgacgg cctgctggcc gaggtgcccc gggacggcca cgacaccatt ccccatttca
    17701 tccgggcctc ggtgcgtacg cccgacgact ggcgccgctg caaggccgag cgtttccgcc
    17761 tcgacgaccc ggcccgccgg gttgacgtgg aatccctgaa gcgggcccac ccggccggcc
    17821 gcgattaccc cctgggcatt cattgcggtt cgatgatcgg ccggattcgc gacctgctta
    17881 ccatggaggg gctggtctac gccatttacg attacccgga gatggtcgag gagatggttg
    17941 agaccgcctg ccgcctggtc gagcatttcc tggaccaggt gctgggccgg atcgatttcg
    18001 acttcgcggc cggctgggag gatatctgct ccaagagcgg tccgctggtg ccggtccgct
    18061 tcttccggga agtggtggtg ccccgctatc ggcgcctcgg cgaccggttg cgggcggccg
    18121 gcgtcgacat ctggtatacc gactgcgacg gcgacgtgcg gccgctgctg ccttacttcc
    18181 tcgaaggcgg catcaactgc atctttccct tcgaggtcaa cagcagcggc cacccggccg
    18241 aatccctggc taaacacgct cccgaactgc gcatcatggg cggcgtggac aagctccagc
    18301 tgcggagcgg ccggccggcc atcgacgcct gtctcgagag gctgatcccc ctggtgcagg
    18361 ccggcggcta cattcccttc tgcgatcacc gctgcccgcc cgacgtcaac cccgacgatt
    18421 acctctacta cctggaccgc aaggaagaac tcttcgggat gaagtaggcc cggcctttac
    18481 cgggctcagt cccccgaatt ataatattca tgtccgccgg cggctccgtc ggggccgtga
    18541 tcggcgcggg cgaccgaacg aggatggaga tgaccgaaaa ctacctttcc tggccggagg
    18601 gccggggccg gctggaagag tgcttcctgg acgagctggc cgaccgatac gggacgccgc
    18661 tctacctcta cagcgaaacc gggctgcgcc ggcggctggg ccgtttccgg gccgccttta
    18721 aggacctgga cccgctggtc cgctacagct gcaaggccaa cgccaacacc cacctgcttt
    18781 ccatcatccg ggccgagggc ggcggcgccg acgtcatctc ggccggcgaa ctgatgctgg
    18841 ccctggcggc cggtttcgag gccgccgacc tgattatgga gggtccgggc aagcggccgg
    18901 atgagatggc cctggccctc gagaacgggg tcgccttctt cagcgtcgag tcggaggccg
    18961 agctggagag cctgagccgg ctggccgccg aacggaagct caaagccacc atcctgctgc
    19021 gcctcaaccc cgatatcgcc gccggcggcc atcaatacat ttccaccggc cgtaaggaga
    19081 acaagttcgg cctgaccgag gccgaggtga tttccatttt caaggcgccg gaacgctacc
    19141 ggccgctgcg catcggcggc ctgcacctcc acctcggttc ccagatcgac actcccgaac
    19201 cctacctgaa ggcggtccgc gaggcgaagc gcctccacgc gagccacccc ttcgaatatc
    19261 tcgacctggg cggcggtttc ccggtcgttt acgacaagga agtcaccgag ctggagctct
    19321 tcgccgaggc catcggccgc gagctggacg gactgccggt gcgggtcatc ttcgagccgg
    19381 gccgctacct ggtggcgccg gtcggtttcc tgttgacccg gatcacctac cggaaggacc
    19441 ggccgcaccg ccgcttcctg gtggtggatg ccggcatgaa cgacctcatc cgcccctccc
    19501 tttacgaggc cttccaccag atctggccgg tccggagttc cggggccgcc ccggagaatt
    19561 tcgacgtggt cggaccgatc tgcgagtcgg gcgatttcct ggccagggag cggcccctgc
    19621 ccgcggacct ggccgccggc ggcctcctgc tggtcggcca ggccggcgcc tacggttttg
    19681 ccatgtcctc caattacaac ggccgcctgc ggccggccga ggtgctggtc cgcggctccg
    19741 agagccgggt catccgccgc cgccagaatt acgaggattt gctggcgggc atgtaaagcg
    19801 cggcggtttt cggttcgcgt ccactctgcg tccggggcaa aagctacgcg actcgggaac
    19861 gaggactctc cgtgttcgcg gccctggtaa agcgggccgc gccccggata ctcattccct
    19921 cgtttcgctt gttttgcacc cggactacgc gacgccggtg gtttttaccg gcgtgggggt
    19981 gagagagggg ggcagtggcc ccttctcgtg gtcgtatccg gggaggggga atcaggggag
    20041 ggaagctcct tcccctgaaa gcgagccccg attgcgtttc ggggcgtagc gacgctgttc
    20101 accgaagacc gccgcttaag gggaacagac gaaagacaac tacataacgg cccgggtccc
    20161 ggctactcat tgcctcacac taattgtttt attaccatcg ctaagaaatc gacaacacca
    20221 ccgcggaaag attcttcaag accttgaaaa cggaaggagt ttatctatgg gaatacggaa
    20281 acctcacaga tgttcaggaa tggattccat actttctgga ggtggtgtat aataggaaac
    20341 gattgctctt ttctctggga tatctgccct cggagaaatt ttaaaaccat ctgcttgtca
    20401 aagaacaagg gcttcggccg cagacagatt attttaatct gcctgtccaa ataggtgttc
    20461 acttcaatcc cgacttgttc ggcaggcagg agtgttgaca ctatcggccc atgctaaaga
    20521 agagaaaaga acccctgcga tatgacatgc tggccgacaa tgaatcggtc tatcgtgcca
    20581 gacagaaggg actactgcgg gagtccgttc ggacataccg actcgttgac cttttcgctg
    20641 gcgcaggcgg tctgacgcta ggcttcacca agacactagg ccaggtcttc gtgccggtgt
    20701 gggccaacga tttcaacacc tacgccgcca gaacttacaa cgttaacttc ggggatcact
    20761 gcaccacgga cgatattctg gaaattgtcg agaaccgaat tgacgaaata cccaaagcag
    20821 acgttgtgat cggcggtccg ccatgtcagg ggtttagcct cttgaacaaa caacgagaag
    20881 gtgatccccg gaagcaactt tggcggcctt attttcgcat tgtagaacgc actggtgcgg
    20941 aagtgttcgt gatggaaaac gttccgcaac ttctcggttc tgaggaatac gaggaaattg
    21001 cggaggttgc gcattccctc ggtttcaaga tggcatttgc gaaactgtgc gcagccgact
    21061 acggcgtgcc gcaaacgcgc tggcgcgcgt tcattatcgg atgcaaattc gcacatccgg
    21121 gtaagttttt ccccccgaag aaaacacata agcgtcctgc cgacgtaaac acaggcatgt
    21181 tgttttcaga ggaattcgat cagtacgttt cgcgggctag accttggcga acggtacacg
    21241 atgcgattgg cgatctgcca cccccgaagg gaaccgaaat ccgaaatgaa ccaccaccac
    21301 ttgacctgca tttcggacgg actcctacgc ccatgagcat gaagcgttac ctgtcgattc
    21361 ccaaggaagg aatgaaccgg ttcgatttgc agaagcgggc accagaattg actcccgctt
    21421 gctggatcag aaaaacggag ggagggaccg acctttttgg tcggctttgg tggaatcggc
    21481 cagccttcac gatccgcacg gagtttttta aaccggaaaa gggacgttat cttcaccctt
    21541 gtcagcaccg gcctatcaca caccgggaag ccgctcggct gcaatccttt ccggattcct
    21601 ttaagtttat cggatcgaag atcgaaatcg ccaaacaaat cggcaatgcc gtgccgccca
    21661 tgctggcggc ccgaattgcc gactgcgtgt ttgcgctact ggcgaccaaa gaggaagggg
    21721 atgtcggata aatttccacc ggaaaaacga agccagataa tggcaaacgt caaagggcgg
    21781 aatacgtcgc ctgagcgtgt tgtcagagga tgtttgcgcc ggttgcggtg tcgattccgg
    21841 ggaaacgtca aggcgcttcc tggcaagcca gatgtggttc ttaccgggag gggcaaagtt
    21901 atttttgtcc acggttgctt ctggcacggc cacaggcgtt gtcgtcgatc aaaacgtccg
    21961 acaacgaacg aaatgttctg gaacaagaaa atcgacggca acattcaaag agacaagaag
    22021 gtgcgccgcg ctctcactcg tttgggatgg aaagtccttg tggtatggga gtgtggaact
    22081 cgcaatccga cgaagttact cgcgattctg gaaaggttcg taaatgcgag atagaactag
    22141 aaaagaaacg aacagcaccc ccgaagccat tcgatgtcag cttgaggaac tgataagcaa
    22201 cttcgaactg catcttcgtg caggtgagct acgccctaaa gtgctcgcct tggttagcat
    22261 ttttcaaggt ttgagaaacc ttggtaaggc aatcattcct cagaagtatg catcagcagc
    22321 aagagatcgc atcttgtact acttccgcaa gtacccgaat gtaatcataa acggtgatga
    22381 attactcgtc gtttccggaa ttcaggaata tgctcgtcgc ttacgcgaat tacgcattca
    22441 gcagggttgg gcaattgcca gcggcgtaac aattaaagac atgtgcgaga ccgaatcgga
    22501 cgaagttccc gatgatctga aggccatgaa gccggatgat tatgtgcttc tttgcgatac
    22561 ccaagatcgc gatgccgcat accgctggca tctagccaat accattcgta aggagcgatc
    22621 gtctgtacgc gacaagattc tcaagttcat gcgcgcaagc gttgggcaag gggttactaa
    22681 cgaagaactg cgttacgtag ccggagacaa aacggaatgg gctagacgtg ttcgggaact
    22741 gagaacggaa ttcggttggc caatagttac gaaaacaaca ggccgccctg atttggacat
    22801 tggcgtctac gttctccaag ccgataggca aagtcccgag cacgatcgaa cgattccaga
    22861 cgacgtacgt aggcatgtga tgcgtcgtga tggttacaaa tgcacgaagt gcgcttggtc
    22921 ccatgcagaa tggaatccgt ctgatccgag acacctagaa attcatcatg tgaaacacca
    22981 tgccaaaggt ggcgataatg aggaacagaa tctcacaaca ctctgtatag cctgtcacga
    23041 cgcaatccac agataggcga gtcagagaag cgaacaatcg gttcctgcgt acggctcacc
    23101 gccgttgcag agccgtgatg aacaggaatc gtccgttagg ttgaccttct cctccactcc
    23161 catccacagt caatcttatc ttcaattgac aaattacgaa aggggactaa aatattaagc
    23221 gtggaaaagg ttgaaggcca aaggttttcg cttgatcaag gagggggaag atggaaggcc
    23281 aggcgataaa accgaccggc tgctgcgatc cgttcgatcc cggcccctgg caggagaagg
    23341 agatcacctg gcgggagaag ctgttcgtga aggatcacgt gacgagtttc cttcacgttc
    23401 cgctgaatat gggcgggaag atagtgaaaa acctgggatt gatcgagaag gccagcgccc
    23461 gggcaacccg gttgatgctg accgacgaga aatccacctg gggttccgac atctacatcg
    23521 acgtcgcccg ggaggtgccg ggcgcgcaga tggccactat ttcgggcacc ttcctgacga
    23581 aggtcttcga aggtccgtat cgaaacgccg gccagtgggc ggaagaaatg aaggcgtacg
    23641 tgaagggcca gggcaaacag ttgaagaaac tctatttttc atacaccacc tgtccgaaat
    23701 gcgccagggc ctatgggaag aattacgtcg tgctctttgc gcaggtagac tgaagcgggt
    23761 ccgccgtcag gcggattgag cggttgccag ggagggaagc atgtcgaaat cttgccgggc
    23821 ctgcggaatg ccgctgatcg aaaaggagga tttcgccggg ggtaaggaag acgccctttt
    23881 ttgccgtcac tgcgtaaacg ccgacggcag cgtcaagtcc ggcgaggaga tcttcgaggg
    23941 cggggtgcgg ttttttatca gccagctcgg tgacgaccgg aagctggcgg agaggataac
    24001 gcaaaagaac atggccgggc tgccctactg gcagggcaaa aactgccggg tcctgcaggg
    24061 cgaaatggcc accgacgaag aatttgccga ggttttaaaa aagctgggcg cgtagtctga
    24121 actgcccggg ccgcttcccg ggcgacgcaa cagcaagaaa cgccgaatat tcgatttggc
    24181 gaagttccgt tcttccccgg ctttgctttc cggaatccgt ttctccccat ttccgaccgt
    24241 tggaggagcc atgaagaagg tttcgatcgt ttccaccagc aggggaacgg ccgccggact
    24301 gcaggaggcc ctggaccggc tcggcggcct ggcgcgtttc gtcacccggg gcgaccgggt
    24361 catggttaag cccaacctga acggcgactg cgtcaccgac ctcggtctta ccgaggcgct
    24421 tatccgcctt ctgctcgatc tggcgccgaa ggaggtcttt atcgccgagt cgactttcgg
    24481 tgacgccgcc aatacccgct tcttcttcca gaagagcggg tacgccgagc tggccggccg
    24541 ctatgggctc cggctggtca acctgaacga gtcggaggcc gtcgagacgc cggtggcccg
    24601 gccgctggcg ctggagaagc tcaagctggc ccgggaggtc ttcgaggccg accgcctcat
    24661 caacctgccc aagatgaagg tccactacgc caccggcgtc tccctggccc tgaagaatct
    24721 caagggactg ctggtcggcg atgagaagaa acattttcac gacgtcggcc tcgaccgggc
    24781 catcgtcgac ctgaacaaca ccgttccggt ccatctcaac atagttgacg cgatcaccgg
    24841 catggagcgc ctgggtccgc atggcggcga cctggtccgg ccggacctgc tgatcgccgg
    24901 cgccgcggcc tggcggaccg actgcgtcgg aatccgggtc atggattacg agctgaaaga
    24961 ggtcggccac ctggcccggt atcttgaggt caacgggctc gacccggcgg atgtcgaact
    25021 ggccggcctc gggatcgagg cggcgcgcca tcccttcaag aaggtgaaca tggccaacct
    25081 gctgccgccg aatatcaggg tgcggcagcg gaacgcctgc tgcgcctgcc tgaacgccct
    25141 catcctctcc ggccgattcc tggagcggcc gccggccgca ccggttgagg tttgccttgg
    25201 ctccctcccg gaggcgtccg tggccggctg tccgcagatt gccttcggca actgcgccgt
    25261 caaaaggtgc ggtccgggtc tgccgaaggt cgaaggctgc cctccttatc ccttcgagtt
    25321 gaagaaggtg ctggaaagaa atttcaaaaa agccgaaggg aattgaaaaa aggaaatcaa
    25381 ttcctatgtt accgatttcc aatcactgtt gacggagcag gaaatgtcga tactggataa
    25441 cctgcgtgaa aaacaccggg ttaaattgtc agaatcgtcc aggcgcaaag agatatcctt
    25501 ttcattccgg gagccggacc gggtccccat ccggataagc gccgccggca gttattacgc
    25561 tcacatgttc gggtacaaca tcaaggatta ttaccaggaa gcggatgtcc aggtggatac
    25621 ccagctgcgc ggaatagaat ggcggttgga gaatcttaag gatgataaca ccgacacgga
    25681 aatacacctt gacatcggtc cgatttcgga aggcctggtc ttcgattgcc cgatcgaaag
    25741 gcccgacggg acttctccac ggatcgtgcc cattcttaag gatgaacatg atttcgcgga
    25801 attgcgggtc ccggacccga aacgtctcaa gggactggag tggttggacc gccggttcga
    25861 ggatttttac cgggcggcgg cccgaaggga gtcaacctgt ccagaagcgg tccccggttg
    25921 aatatacacc cacctttgtc cgccgcctgc gccatcatgg aacccgcccg ggtgtacttg
    25981 ttgatggctt tgaagccgaa aaccgtgaaa gagttgttcg ccaagatgtt tgacaccttc
    26041 tgcgcactgg ttgattatta cgacgaacgt tatggcgtcc ggaccgaaac cctggggctt
    26101 gccaacgaca acacctgttt tatttccaac gccatgtatg tctgccaggt cctttcgtac
    26161 gacaaggccc tgtacgaacg atacgggttg aaaggccgct cgatgcatac cgacggtccc
    26221 tcggaccata acttcaagac gttttgcgag gaattcaagc tgaacgttat ggacatcggc
    26281 ggttggagtt ccatcgacgc cgccgttgcg gccatgaagg gcaaagtcgt cattcacggc
    26341 ggtcttaatt gcaaggacgt ttacgggtcc ttgaccgacg cggtcaaaag aaagatcagg
    26401 catgcgataa gggtggccgg accgggcggc gcgtatgaat ttgccatcgg cggggaaact
    26461 tatgtgggcg tccccgcggg aacgctcatc gacctggtga attacgtaaa gacggtcggt
    26521 aaatatccgt taagcggcgg gacggaatca actcctggtt cctgacggtt cggttcccgg
    26581 cccgccgccg gattgaaacg cgccctccgg tacggtttcc cgcctgtcag ccgatcgttt
    26641 caagtatcgt ttcgccggcc gcccggttcc gctctttttt aaagcagttc ctgctctgtc
    26701 ccggcaatgg ccgcctcgat catcccgacg cccttgaggg ccagctcctc tttgatgacc
    26761 ttgagggtgg cggcggccga cagggttgaa ggccggcccc gggccggtac cccagccatc
    26821 accctgactt ttcaaggctg gttttcttga ccaccggctt tcgggatgat agtattaaaa
    26881 gatagcctgc cgccgatttt cagcccgatt gacgttttca gccccgggca aagggccggt
    26941 tttggcagtc ccggtgaaag gccgtttccg ggaccggcgg cccgggttca atcttactga
    27001 ggtgtccgag gcgatagcgt gaaacttaag acgaagctct tccttatctt cacctccacc
    27061 tccatcctga tcattttcac cttcggaacg attatctaca agcggctctg gaacgagcag
    27121 ctggccctga tccaggcgaa cctctcccag cagcttcaga atttcgattt ctccctgaag
    27181 aatttctttg ccgaggtgga gggcgacgtc aacgccctgg cgcagaatga ggtggtccgt
    27241 tcccgggatg acagccagtt caccagtttc ctgcaggcca acgaaaagaa cttccggtat
    27301 aactactcgg aactcgaaaa aaagatcatc cggatcttca atgcctaccg gcttacgcac
    27361 ccttacgtga attcggttta catgggccgg gagaacggca gcatggtgcg ctcccatccc
    27421 tggaaccgac ccatccgcta cgatccccgg caacgcccct ggtatattct gggaaagaag
    27481 aatccggccc gggttgttac gaccgaggcc tacccgtccc tgacgaccag cgacatcaac
    27541 ctcggtttcg agaaggccct tcttgacgaa aagggacgcg tgtacggggt ggtgggcatc
    27601 gacgtcacgc tggtcaacct gaccgattac atggtcaact tccaggtccg tcccgccgga
    27661 accatttttc tggttgaccg gaacggcgtg atcatggcca gccaggcggc cggcctgcag
    27721 ggccggaaga tcggtgagta tgcgccggcg ctcccgtccc tcctggcccg ggacgagcgc
    27781 gagatcgctc ccatcgagat ccgggggcgc cgccatttcc tctttcaccg taaatttgcc
    27841 gagcaggatt ggagaatcgt cgttctgatc ccggcggcca acattgaaaa aatgatcggc
    27901 gatcacgtcc tctggatggt tctgagcctt tgcctgggcc tggccctgct cagcctgctc
    27961 accctttcgg gtctccacct ttttgtcatt catccgctca agaagctcac cgaaggggtc
    28021 gatttcataa cccggaccgg tgacctgggc cgccgcatcg gcatcgcctc acgcgatgag
    28081 ctcggcaccc tggccagctc cttcgaccag atggtcgcca ccctggaaga atcccagcgt
    28141 tcgcttcagg tgaaggagaa ggtcctgacc gaataccgcg accatctcga ggaactggtt
    28201 cggcagcgga cgcgggaact ggaggcgacc ctcggagaac tggcggtggc caaggaaaag
    28261 gccgaggcgg ccgaccgctt gaagtcggcc ttcctggcca ccatgtcgca cgaattgagg
    28321 acgccgctta actcgatcat cggattcacc gggattattc tccaggaatt ggccggcccc
    28381 ctgaacgagg agcagcgcaa gcagctcggc atggtgcgcg acagctcgcg ccacctgctg
    28441 gccctgatca acgatgtcct ggatatctcg aagatagagg cggagcagct ggagatacgc
    28501 aacgaacctt ttgacctgcg ggcctcgatt ctcaaggtcg ccggaatcgt gaggccgctg
    28561 gccgaaaaga aacaggttga cctgcgcctg gaaattgccg cggagatcgg gaggttctcg
    28621 agcgactcgc gccgggtcga gcaggtgctg ctgaacctgc tgaacaacgc cataaaattt
    28681 accgagcggg ggtgggtgca gctcacggcc gcccttgccg cggaagggaa cggcaccgcg
    28741 gtccggatct cggttgccga taccggcatc ggaatcaagg ccgaagacct cggcaaattg
    28801 ttccagccct tccggcagat cgattccggc ctcgcgcgcc agcacgaggg taccgggttg
    28861 gggctggtca tctgccggcg gctggccggg ttgctgggcg gggaaatccg ggtgaccagc
    28921 gagttgggca aggggagcgt tttcactttc atcctgccgg taccgtcagc ggagacccca
    28981 tgaagaaaag gattctcctt attgaagaca acgaacaaaa ccgttacctg gccgcttttc
    29041 tgctcgaagc gcgcggctat gaggtcctcc aggccgccga tggaatccgg ggcatcgaaa
    29101 tggccggcag cctccggccg gagttgatcc tgctcgacat ccagcttccc ggaatggacg
    29161 ggtacgcggt cgcccgcgaa ttgcgcgcca accccgcgct caagaccatc ccgatcgtgg
    29221 cggtgacctc ttacgcgatg gccggcgacc gggaaaaatc gctggccgcc ggctgcaacg
    29281 gctacatcga aaaaccgatc aacccggaaa ccttcgcggc cgaagtggca tcttacctgg
    29341 aaaaggattg aaaggagatc tcttcatgaa gaagcggata atgatcgtgg acgacaagga
    29401 ggaaaacctt tatttcctga acgctttgct gcaggggtac ggctacgaag ttgttgccgc
    29461 cgcgcacggc gccgaggctc tcgaaaaggc ccggtcgcag ccgccggatc tgattatttc
    29521 ggatatcctg atgccggtga tggacgggtt cgccctctgc cggaactgga agaaggacga
    29581 gcgattgaag ccggtgccgt ttgttttttt acaccgccac ctacaccgac gagcgggatc
    29641 gggatttcgc gctcagcctg ggcgcggagc gttttgtcgt caagccgacg gagcccgact
    29701 cgctgatggg gatcatcgag gagacccttc ggcagagcgc ggcggccgtt ccgaagacgc
    29761 cgccccggcc cgcgggcaag aagtcccaag aggagaccgt ttacctgagg caatacaatg
    29821 aagcgattat ccgcaagctg gaagacaaga tggagcagct ggagcaggtc aaccgaaagc
    29881 tggaacagaa cattgccgag cggaagcggg ccgaggcggg tttgcagacc agttacgaaa
    29941 agctgcagaa aatgatagac gagacgatcg aagccctggg caaaatgggt gaaatgcgcg
    30001 atccgtacac ggccggacac cagagccggg tggccaggct ggcggtgacc atcgcccggg
    30061 agacgggggc gctggaaaag gagatgatgg tcatcggctt gcttcatgac gtgggcaaga
    30121 taatcgtacc ggtcgaaatc ctgaccaagc ccggcaagct gaccgaggtg gagtttcaga
    30181 tgatccagcc ccacagccag gccggttatg acatcctgta cggcattgaa tttccctggc
    30241 cggtcgcgct tacggtattg cagcaccacg aacgcatgaa tggttccgga tatcccaacc
    30301 ggctggccga gaaccagata atcatggaag ccagaatact ggcggtggcc gacgtggtgg
    30361 aggccatggc ttcgcatcgt ccctaccggc tggcggtggg tactgagaat gccctggaag
    30421 aaatctccgg aaaccgcggc gtgctttacg atcccagggt cgtggatgcc tgcctggggg
    30481 tcttccgaaa agggttcaag ttcgattgag gcctttgcag ccgggcgggt cttccaccgg
    30541 gggcgttgtg atttcggggt cccggtttgg ccggccggac tgattacttg aggagaccgg
    30601 gcatgcctct caacaaaata ctgcacctga ccagcggcga ctgcgccggc ggtcttctgg
    30661 cccgatccgg cctcgagggt gagatcttcg tctggcacga catcctttac gacggccccc
    30721 gccggcccgg ctggccggac gaggcgatcc tggccgcccg ggcgcggttc atcgaggcgc
    30781 agaccggccg gggtttgagc cgggagaaaa tcctgaagtc cttccgctgc cagtacgcca
    30841 ggctccgaac ggaagccggg tacgggagga tcgtgctctg gtttgacgcc tgtctcttcg
    30901 accagtccat gctcgtccac atcctggtct gcctgctgga gctgaaccgg accgaaaatg
    30961 tcgaattgct ctgcctggac gctttcccag gcattgttcc cttccacggt ctcggacagc
    31021 tgacggccgc ccagctgctc tccgtgtacg gccggcgccg gccggtgacc gaagcccagt
    31081 ttctcttcgc gaagcgggtg gatgcggcct tcgccctcca ggataaacag gccttcgccg
    31141 aactggcggc cggcgtcggc gcgcccctgc cctgggtgcc ggccgccgtg gcccgctggc
    31201 tgcaggaaca gccggacgga gcggacggcc tgggccggct ggaacggctg gcgctggagg
    31261 cggtccgcaa cgggaagaac cggccgcgcg agatcttcgc ggcggcggcc gccggcgaca
    31321 ccccgcccca gttctggggt gacacgacgc tctgggcgaa gataaactcg ctggctgaca
    31381 aggagctggt gttgatagaa ggtccgtcgc cgcgcctgcc ccagtgggag gccggcctcg
    31441 acctggaccg ttttcagata accccggctt gataagaact tatttccgct ttattccttt
    31501 ttgaccaaac ccgcccgatc attttcagcc cttttaccgc cggccgcctg gaggtagagg
    31561 acagcggccg ggatgaaggc cagcagacag accaggtaaa cggcgccgcc gctgattttt
    31621 ccgtggagcg ggttggccgt cagcggatag aaggggcgga tgtcggagta gaggggggcg
    31681 tccagcagga cgtggaacca ggcgccgagc agggcggaga tcgccatgat ccgaaaatcc
    31741 ggccggtagg ggaggcgcag gcggttcatc agccgccgga ggtgtcttcg acccgcgaag
    31801 gcgagggccg cccagccgat tcccaccagg ctcccgaaga ggagggtgtg ggcgtaaccg
    31861 tggaggggat acttcaggcc gaatgagatc acggccaggg gttccaggtc gatcgccagg
    31921 ttggagagca ggaagaccgg caggtcgagg cgttctttca ggggcagggc gaggcaggcg
    31981 ccgggtccga agtggaacgg agtgaaaggc atcggcttct tcctttcttc aagcggaccg
    32041 acggcccggg cttcccggcc ccgggcggcc gtcaggattc cggccgcccg ggactgcttc
    32101 gaagcggttt tctccgccga agccttaaag cagttcccgc tccgtttcgg cgatggcctc
    32161 ctcgatcatc ccgacgccct tgagggccag ctcctcgttg atgaccaggg gcggcgacat
    32221 gcgcaggatg tggccggccg gtatccaggc cagccccttg cggaaggcct tctggtagac
    32281 cagcttcccg gccgcctcga agggctcctt gctctgcttg tccttgacca gctcgacgcc
    32341 gaagaggcag ccgcggccgc ggacgtcgcc cacgatgcgg tacttctgtt tcatcgcctc
    32401 cagccgcttc atgatcacgg cctcgatttt ccgggcgttc tccaggagct tctcctcctt
    32461 gatgaccttg agggtggcca gggccgccgc gcaggccagc gggttgccgc cgaaggatga
    32521 ggaggcgctg atcttctcca ggtgctcctt gtagggttcg cgcaccgcca tcaccgtcac
    32581 cgggaaaccg ttgcccaggc ccttgcccag ggtgatgatg tccggcacca ccccgtaatg
    32641 gtcgcaggcg aaccaatcgc cggtgcgtcc catgccggtg aggatctcgt ccaggaagag
    32701 gaggatgccc agcttctcga ggcgcttctt cagttccggg atgaacgcct cgggcgggaa
    32761 gacgctgccg gcccagccct ggatgggttc catcaccacc gccgccaccc ggccggtggt
    32821 ctcctcgcgg agcacctttt cggtgaagtc cacgcagtag aggttgcagg cgggatgctc
    32881 catcttgaag gggcatcggt agcagttggg ccggggcgcc aggaagaagc cgggggagcg
    32941 gatgcccttg tccgggctca tgccggccag ggagaccgag ccgagcgtct tgccgtggaa
    33001 gtccttccag aaggagagaa actcgaagcg gccggtgatg gcccgggcgg cccgcaggcc
    33061 ggcctcgacc gccggggtcc cgtcggaata gaactggacg ccgctgatgt ccccgggcag
    33121 gacgtccacc agcagttcca gcgcctcgac tttgaccgag gcggtgaagt catgggcgtt
    33181 ccagagccgg tcggccatcc gcttgagcgc ctcggcgatc ttgggatggg cgtggccgca
    33241 gccggtcacg tagatgccgg aggagaagtc gatgtaggtg ttgccgtcgg cgtcgcgcag
    33301 gaaaggaccg cgcccctcct cgaagacgac cgggaagagc tggacctggg aggagaagcc
    33361 cttcatgtac ttctcggccc ggccgtggat ctcccgggat cggggcccgg gcagttccgg
    33421 gcttactatc cggggtgggt tctgcacgta gtcggagtac cattcagcca tcgttttctc
    33481 cctctttttc gagttgccgc ttgagacggc ggtagagttc gtattttttg acgtaagctt
    33541 cggtccggcc ggggtggggc cggaaacggc gcccggccaa tttgaagcgg ctctccggat
    33601 ggcgtccgga gaggccggcc agggccagcc gggccgcgcc gcggccggcc gcctcgctga
    33661 tctccggcac cgtcacttcc aggccggtga tgtcggccag gaggcggggc aggctggttt
    33721 gccggaccgg gccgccgatg gcgaccagtt ttctgacccg gtccccgccc agccgggggg
    33781 cggctaaatc caggtactcc cggatctgga ggccgagtgt ttccatgacc gcccggagga
    33841 tctcggccgg tccgtcggcc aggcccaggt tcaggagtcc gccccggccc cggatgaagt
    33901 tgggcaggac caggaccggc gagggcgttt cgccttcggc cggccgggtt tgtttcaggt
    33961 tgcggctgaa ccaatcgtaa agaattccgc cgttgctggc cgcacccatc aggccgaaac
    34021 ggcccggcag caggtgcggc ccgggcgcca gccgccgggc cgggtcgtag accggccgcc
    34081 gggtggtgat caggattacc caggcggtgc cgcaggagac cagggcggtt ccggccgccg
    34141 cggccccggc gcccagtgcg gcgcagtact ggtcgtgacc gccggcgaag accggcgtgc
    34201 ccgcggacag
//
LOCUS       DAOQYN010000002        25400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_2275_length_25400_cov_4.504399, whole genome shotgun sequence.
ACCESSION   DAOQYN010000002 DAOQYN010000000
VERSION     DAOQYN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25400
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_25400_cov_4.504399"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PKN80_00165"
     CDS             <1..1192
                     /locus_tag="PKN80_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HNQ34468.1"
                     /translation="FAESRRLLKEDGINSIVFAHKTTEGWEALLSGMIRANWTITGSW
                     PIATERPGRLRSQDSAALATSVHLVCRPRPDDAPVGDWGEVLRELPKRVGNWMERLQG
                     ENIRGADLVFACIGPALEIFSRYSRVETAEGKEVKLAEYLEKIWEVVGRTALENILGT
                     AEAKARNGMAGALEEDARLTALFLWTLQSTNGDAEKIETEAVTDTDPPTEDDEQDSQE
                     SSKGKKKTGYSLIYDVVRRFAQPLGIHLEEWEDRIIVTEKGVVRLLPALERATLLFGE
                     KGAQFAAKQLEIKQTDINQVLLPGFERKAIAPEIRGRRRGGPNIKEDEVSHRRGATTL
                     DRVHAAMILQSGGQTNALRALLKAEVERGPDFLRLANALSALYPKNSEEKRLLDAMLL
                     AIPR"
     gene            1210..3954
                     /locus_tag="PKN80_00170"
     CDS             1210..3954
                     /locus_tag="PKN80_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HNQ34469.1"
                     /translation="MEAWYKIATPRKEVRQGRSFNPDEFAIHLEQVIAGTAPEDYRDP
                     KQFFVRTCFTRALREHAGMILRRLSGETANTAPVMTLITQFGGGKTHTLTALYHLVKN
                     GNKASEYSGVGDLLKEAGISAVPKARVAVFVGNAWDRKEGHETPWIDIAWQLAGEKGV
                     KELGTAAKTTPPGTDALNRVFQAAASPVLLLFDEVLNFLNRYRGMADQFHSFIQNLTV
                     ATTGTTCGAAVISLPRSQVEMTDWDMQWQDKITKVVRRVAKDLIANDETEISDVIRRR
                     LFEDIGNERIRKNVAKTYADWCFERRAQLPPEWTAVDTTTTEVKAKDYLRSRFEICYP
                     FHPATLSVFQRKWQVLTQYQQTRGTLAMLAQWISWAYKEGYQQARHEPLITLGSAPLE
                     ASEFQSVVLGQLGETRLMTAIDADISGPQSHARALDADTTGALRNIHRRVGTVILFES
                     SGGQVDKMAHLPELRFALGEPSVDTTSVDNAAFALEDKSFFIRKAGSDGFRIGYQPTM
                     KKVVSDRRASLDEESEIKPAMSKLVEDEFRRGANIPIVAFPEDGSAVQDTPKLTLFIM
                     DPLHELTGENTLRGQIAEWIRMRGKSPRLYPGSLVWCLKKPGRDLRESIEMWLAWKRI
                     TREIADGTLGGDFDRSDRAEINSKIAASEDAAKDDVWGGYRFAVIADMKESDGLKVID
                     LGAGHSSSDETLCGRIITALRSQALLNESVGAGYIDRNWPPALKESGAWPLASLRQSF
                     LNGSLTRLIDPDTILRSKILEFVEKGDFGLAYGQKPDGSFERVWFKELISADEISFES
                     NVFLLTKKKSQSLKEGTPPPPPPQTPELNPEPEPGTGPIPEPGAQEKALRIVGTVPPE
                     VWNRLGTKILPKLRSAKDLKVGVEFSVVVTNDLKDNLIADLRQALEDLGLSDKVRIEE
                     "
     gene            3974..4174
                     /locus_tag="PKN80_00175"
                     /pseudo
     CDS             3974..4174
                     /locus_tag="PKN80_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4234..5925
                     /locus_tag="PKN80_00180"
     CDS             4234..5925
                     /locus_tag="PKN80_00180"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNQ34470.1"
                     /translation="MDNSYPTLEPLKFLLQMIHNQEMALPDFQRDFVWDPYATDELIE
                     SIIQNFPAGTLLRIKNGKELLFQPRAFEGAPVLSPQNQPSYLILDGQQRLTSLYQAFY
                     GCGEHRYYLNLQKLEQGLALEDCAFYMRTKDGEAMYGSLEQQARELVFPMQNLFGVSG
                     GFSAWQNEVLRACSGDKSYILNLQEKLSKIHDQWIQPVEEYRFPMVTLNEETSAPAVC
                     MIFETLNRTGIKLGVFDLLTARFWPKDFNMRMKWDEAKDAHSMIEEYKIDPYYLLQII
                     AMLEPGLDKEGKAKVPSIKRGEILNMKVEQARRGWDAAVMGLKNALLMLRDDCGILDS
                     SLIPYNTIIVPMSAILATQTNVKGPEWGANRIKIQKWFWCSVFGQHYENAPNSQAEKD
                     FGELKQWLNGGVPPESVRNFNFDIDLRQVGPRQRAVYKGVMALILKNGAMDFYKRGKI
                     TSQLLADKKNPVDDHHIFPRAFCDTNNMPVSLRDCILNRTYIDRETNHSLNKRKPSDY
                     FREIRDKQGKAEVDDLFKSHAMPIGDTSPVLSDNFNAFLEFRHKAISEMVRQHTT"
     gene            complement(6001..6171)
                     /locus_tag="PKN80_00185"
                     /pseudo
     CDS             complement(6001..6171)
                     /locus_tag="PKN80_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024729.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6270..7850)
                     /locus_tag="PKN80_00190"
     CDS             complement(6270..7850)
                     /locus_tag="PKN80_00190"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNQ34471.1"
                     /translation="MKRLMIGAGITALLLLGLVSKNGPVPGIADEVRNTSPDPLVSDW
                     VRTWGGSSAKDQAQVNKMAADKQGNLYVAGNFSGTVDFNPDPAAFDWRASEAGSIDAF
                     LCKFDPKGKLLWTRTWGGNKRDTAYGVDVDRFGNAYVVGPFRSTVDFDPDPVKTAIQT
                     SNATGGQVYENNVFLSKFAPDGSFQWVRTWGPKIVPGKSAFGAEGYNVAVAGDCLYVA
                     GDFSGDRVDFNPWGGHDWHANHVPATGLVFFDVFLSKFDLDGTFQWARTWGGEGYDDG
                     PGVAVDGEGNVYVAGMYASREINFDPAGGKAGLGYPAQDSGIQVDVFLSKFDAAGRFK
                     WVRTWGSKGSEEAMGSVCVDRMKNVYVCGRFTSNNCDFNPRGKPDIHSTQGGQDLFLS
                     KFDSEGNFQWARTWGGRGDDQGGVTIDRAGNPYAWGWFCDTVDFDPGNGTDRRVSNGK
                     QDLFFSRFDPNGSFNWVQTLGGPDKETGSVMLDDSGNLYAGGSFAGTVDFDPGRGIDS
                     RTASGARAAFLGKFLLRQ"
     gene            complement(7847..8362)
                     /locus_tag="PKN80_00195"
     CDS             complement(7847..8362)
                     /locus_tag="PKN80_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34472.1"
                     /translation="MPFTPLHAGPGLAVKAALPRHFSLTTFGLAQIAIDIEVLVYILQ
                     GKPYLHRFWHTFLGATLIAFLMPLPGKPASQWIKKQWNRATGIPAVPVRTTWTASFAG
                     AAIGAYTHILLDSLFHNDVEPLQPWSSANPLYGLVNPSDVETICVILGIAGLAGLIIR
                     RALRTKTGWGK"
     gene            complement(8529..10754)
                     /locus_tag="PKN80_00200"
     CDS             complement(8529..10754)
                     /locus_tag="PKN80_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34473.1"
                     /translation="MHNSLHIRDLLRKSNADPLCRQRRDPHRLVMRDPAARPASGDLV
                     VGDGAAIFCDWPDPEVAGLVRRDLEDFLGRLGVQTGSAGRPRILLRRSTDGLAERDFR
                     WDSRPDLLAIEAGGVPGLWAAVAWLEFEMGARRGPFLPAGPHEGRAAWGHQISQGPWG
                     GNYSVPDFGPEYLDDDAFRLYAHYGVNEMMIYGDLLCYAESAVLPELNHPEAGRNLAV
                     LQEAARRAARYGVRFSYVAVGPKLREDHPVFAAHPAAGGIVVERDPGLRLRFLCTESE
                     EGRAFYREQFGRLFGAVPELAGLILIVAQESFYHCKMWWPQQREKCPRCEGRSTEEVL
                     AGILGEIRESLRGANPAAYLAAWPYTTTGWERPDRLEFIRRLPPEVAFMLSVEKDQAF
                     RKEGYLKNIWDYSVDYGGPADPMVKCAAACREVKRPLVVKTETGIGLECIQLPYFPAL
                     PRLAAKWEVVRSLSPFAVHQSWLFFGMFNSRAEALGLWAAYAADLPAGTFLKRLAERD
                     FGPAAAPLVLEAWRHLGESAGRLPLLQFNFYYVGPSFLGPCHPLVPEKGLKLSPVFDG
                     YLFYLQEHGETFGAQHVSRTRTCLAIDDFNPSGGLPEPLPGETRSPEEIVRDEYGLAA
                     AAAGRALDCLGRAAPLCATGADRSRLGEETRLTEAVYRANRACWNTARWFIGRNGGDP
                     AAMRLAALDERENALAALPMYAEAPWLEFNRRLDGRYSAAAEMIAEKVRMLDRWLQEN
                     G"
     gene            complement(10841..12769)
                     /locus_tag="PKN80_00205"
     CDS             complement(10841..12769)
                     /locus_tag="PKN80_00205"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNQ34474.1"
                     /translation="MKLFPAGRRLAVFVLAGLCLIFFFDYLGLFDGTDNYSYDLSFRL
                     RGARHPDPRIVIVDIDEETLDALGRWPFPRRNYAAFLESVREADAVGFDVIMDEPSAD
                     DSIFNLAIRQQGRVILPVYFDSEAAPHYPLSAFKPHRTGHIHVEPAVDAILRESFHTL
                     YYQQKGIPSFSSALYETMTDKVMRRGSPSAAVKAEPGRNVIFQVDPTRINFRGPPRTY
                     PYLSMGNVILGDYPSSFFRGKAVLLGASAPGLGDVFTTPFSQHRNRMAGVEVHATMLD
                     NLLNGDSLKVAGDWFRRFLGLFLFFPIFTLLAKSSYRKVGLLWVAGCAIIVLGSLLLF
                     TFARYWIKPTAFFISLNFVFVVAHVCRLDEAAGKLSDKYLSIVSLVGARAPTPPRSRT
                     GLTLPSFLSAEGLNSHIQRLFDVEQQYEGKLQKIIDEKTGELSNALDMLHSMSREIVL
                     RLTLAGEYRDSDTGRHLTRIRLYVKDLSEAMGMSHAFIEEITFTSAMHDIGKIGIPDH
                     ILLKPGGLTPEEFEIMKTHTLIGEKILAGSRYPTIQLSARLALCHHEKWDGSGYPHGL
                     KGAAIPLEARIVMICDIYDALRSRRPYKPEFDHSTAFKMITEGDRKTSPDQFDPDVLG
                     AFTRIAAAFEEIYDRHRN"
     gene            complement(12791..14740)
                     /locus_tag="PKN80_00210"
     CDS             complement(12791..14740)
                     /locus_tag="PKN80_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNQ34475.1"
                     /translation="MFRKMLLGAWLVLFSFALLGTVAAQEEKPLFKVTVVKHDTLIGI
                     CRKVLELPKKWREVARFNSMKNPNLIYPDDIIIIPTDLLKGEPVSASLRFVRGKAEIY
                     SEATKTWRPVTMEDKIGEGDIVRTGADSLVEYQYQDGHTVVQRPATTVQVKKARTSSV
                     SSSYRFLLSAGSTVTRIQKATGKETRFQVETPTAIAGVRGTTFRSTVGPDGVVRFEAF
                     DDPVEVTAELGTVEIKNGEGTLVAKGTAPTAPVKLPAAPVLIQPLAVYQRLPVRLAFE
                     KISGAVSYRVVLARDRDFREPIEQRLINPQDNFELAGLKDGTYYLQASSLDAAGFEGL
                     ASEPVELRVRTNPAAPFTELPRDQAVYYGKDLTCKWLKVADASRYHVQIAEDAAFTRM
                     ARDTSDLTRTEYPVGDLAYNTYYFRVSAVAADNWQGLWSDTLRFELQKPLPATPFTEV
                     PRDQAVYRGQALTCKWLKVADASRYHVQIADDAGFTKLVKESSDITANSYETGDLEFK
                     SYYFRVSSIDVDNRQGAWSDPLRFAMQLPPPKDAPAVEAPTISKTDISIRWQDLGDGT
                     SYRFQLAGEPEFGKVLVDQKVPASEITLEKPKKSGTYYARTAAIDRWNQEGDFSAPQS
                     FDVKPSSNAGFWVTLTAIIFTILVP"
     gene            complement(14940..15665)
                     /locus_tag="PKN80_00215"
     CDS             complement(14940..15665)
                     /locus_tag="PKN80_00215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNQ34476.1"
                     /translation="MPNRKETPPAIQRIDLEGIEIEISRKRIRNLYLRVDSPLGRVRI
                     SAPARMSLEHVRAFARSRLDWIRRQQERARQRPAELPLRCLDGEIHCLWGDRYRLEVV
                     ETEAPAGVTMAGGRLILRVRPGAGVAKRRKVLDDWYRGRLRLAAEPLIAKWEARLGVK
                     VSRLFLRAMKSRWGTCNAAVGSIRINTELARKPLVYLEYIVVHEMVHLLECSHNRRFK
                     GLMDRFLPGWISLRAALNRSARE"
     gene            15796..16212
                     /locus_tag="PKN80_00220"
     CDS             15796..16212
                     /locus_tag="PKN80_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNQ34477.1"
                     /translation="MPSLVKEFLGQKNFAVVGSFREKTRYAYLILKDLLARGYEVYPV
                     NPNLDEVEGRPCYKRISDIPFRIDVVDLVTPPPVTEILLEECRQLGIRRVWLQPGAES
                     EKAIEFCHQNGIKVIHGLCVMLEAGNKKEDLPPPQF"
     gene            16334..16419
                     /locus_tag="PKN80_00225"
     tRNA            16334..16419
                     /locus_tag="PKN80_00225"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16366..16368,aa:Ser,seq:tga)
     gene            complement(17042..17833)
                     /locus_tag="PKN80_00230"
     CDS             complement(17042..17833)
                     /locus_tag="PKN80_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34478.1"
                     /translation="MDNRLPEVDFCGLRISRLVLGANPFGGYSHQSPERDRAMVDYYT
                     VARIKETWRRAEAAGITAMVTNNETPHVVQAVREYRAEGGKLQWIAQLNGRDTEAMLQ
                     SIDKAVAIGCRALYFHGGVMDRVYSARDESALRTWCAHARSHGVPVGVAGHAPEVHRW
                     VAGLDLVDFHAVCFFNCGSLHDGNGDKFSLDDIGPAVECIRAIKKPCIGYKIMGAGRM
                     DPRMSFEYALRHIKPGDVVNVGMFRGDRDDMVEENVALVREILLR"
     gene            complement(17881..19986)
                     /locus_tag="PKN80_00235"
     CDS             complement(17881..19986)
                     /locus_tag="PKN80_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34479.1"
                     /translation="MGKIRMAGAFILAALTVLAGSIESVQCETISTIPLAYIILDASD
                     VEFLNPLLRPTDIVAVRPENLALLDGVTVGRKLLLIGPKHEKTPEVRWQAMKDDEMAA
                     LLAQARRHGVTMLGYNLENSFHAEKLVERERQVAGLARAAGLVYVFGPLMGRLMETGG
                     EAVAGGADMVVVQTQALQKSSGFEVQKVLDVVRRLRKANPDVKVHVQIMLRYRQDREN
                     GPLIPVDKVIEHIGLIAGEAEAVWLFSGPQTTSRFREVFSKLRQAAPVVPPPAGERSS
                     PPPAPPKSDPAAAAPATSSGPGTKAGNNVMPPVPRNARFTTGAWVPFARLGGQTAGDT
                     SPHLARILDEAVVNEEQRRAIQAAYQAGANPAAWQAMAAVLTGEQLDRIGRRQPQAAI
                     INHALVFAVEAQTLKRHPDAAERIKSMVERINASFEAGGIRRRFSVAGVRTYDKQEKT
                     GVRQPANSGGLSLPADYETHPHDYILVAMDENAPGANWPCPWCSSVEVHGARGDSGTG
                     KDMFVERSALVLCHELGHMLGLPDFYALGIEAKDNAVNHEPIPSDNYGQVRGTMMDDL
                     GPFHAWDAEIINREIATLPVVNHSWIDYQPRDSVLQVLGKDGAPLRQAEVKVYRSRRT
                     NYYRQALDGTPRHQGKTDDAGKLSLGPNLLGADPFQALCFFLVEIRQGERVDYRWFSF
                     IEVNFSFWRGTPITLQSRL"
     gene            20224..21903
                     /locus_tag="PKN80_00240"
     CDS             20224..21903
                     /locus_tag="PKN80_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34480.1"
                     /translation="MKNNHKGLQLTAWMLSMLGLFWLAGQPPAAAQENKKGIDMKKGE
                     RVLFFDDRYVEEMKELTRVMHPAVKHAGNPVLKADKPWEVSDRKAGIGIVHVYGTVRK
                     EGDLFKIWYQVYPRSGKLYWVCYATSKDGIHWEKPALDLIEYNRLKRGEFTEEEVKGN
                     NIVLINCGRPGATMACSPTISVDPDEPDPNRRYKMLFWDFGVRYYEPGQYMGGCVAFS
                     PDGIRWTPYPGNPVLVASDVTDATWDPVERRYIAHFKLYQLLTEAADPARPVEMEKPA
                     EFFLVGRKKPTQEKDTILAEAYFLDTTLQQVTLETRTLRNCRRISDRRIVARAESKDF
                     INWTNYRAAIIPDRLDAANQQTYGMSAFRYEGLYVGLLRIMTGQSIDIQLAYSNDGAD
                     WQRCFDRTSFIPCGAAGGWDAGMVLTASEPVLVGEELWFYYGGWQKQHHEWGETSIGI
                     AKLRRDGFVSLDAGEKEGELLTRPVTVSGPRLKVNAAAAGGAVAAEVLDESGRVIPGY
                     GRKECRPVAADTTAGELTWAGNRNLSALAGRKVRVRFFLKQAHLYSFWQGE"
     gene            complement(21879..22967)
                     /locus_tag="PKN80_00245"
     CDS             complement(21879..22967)
                     /locus_tag="PKN80_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34481.1"
                     /translation="MGTWTYLSPFSLGYKLSDDFLTPYAPPLWFALFSSADVFIDQDQ
                     EGDFFASVETSAGKARGRVRKILRRIRGDAYAETLLAPLRFFELALSGAAAGETVQFD
                     LLEWGDDFSLAEARQHVEGLTEYMTPFDDFIQGLSPFARLLKLLSGRFCLGSLEKDKR
                     ALEGITQNLEDEAGMKMSLAEYAFGYLAGFPVTDDSPEGKRYYAWVEANRPLWEGGSG
                     VKGPQVPPAEEAVGEPVAEAGPAEEAPPEKERNGLVREYYDSDGPAYCSGRGRLASVS
                     RYKDGRLHGLCKRYEYLGKKRLLVSQLRYKNGRLDGLCKYYVEGELVRSSHFRNGRLN
                     GIVKNFGSEYLFKNGKIVKTLYSPCQKE"
     gene            complement(23241..24791)
                     /locus_tag="PKN80_00250"
     CDS             complement(23241..24791)
                     /locus_tag="PKN80_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ34482.1"
                     /translation="MDQEKAIALLNQAGKKAYLEEKFEEALASCEKTLELDPDDIEAL
                     YNKGLVLCRLDRFHEALEVCDKASRLRPDKGIIFIVKGFALDSLERFNEALAAYEKAL
                     ELDPEDADTWEKKGNALYNLGRFKETLAAYEKTLELNPKQVNAWRKKGDALYNLDRLN
                     ESLAAWDTALELDPKDDAIWYNKGCLFGRLGRFNEALAALEKSIQLNPKDADAWRKKG
                     VVLCDLGRFKEALAACNKAIKLDSENILTWVIKGAILENLGRFKESLAAWNKALELDP
                     EQANSWKNKGDALYNLDRFKAALAAWEKSLDLDPKQADTWKKKGDALYNLGRFNESLA
                     AYEKALKLDPKNGAAWHSKGLVLHELGRFKAALAAYEKALELDPKHADTWYQKGYLLY
                     SRRRFKEALTAYEKVVKLNPKDAAAWNNKGRLLGRSGRLKAALAAFERSLELDPKDED
                     VWHSKGRALYGLGRFKEALPIFEKALKLNPKNVHAWHNKVLTLEKLGRFKDARVVREQ
                     ASRKGFSF"
     gene            complement(24907..>25400)
                     /locus_tag="PKN80_00255"
     CDS             complement(24907..>25400)
                     /locus_tag="PKN80_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34483.1"
                     /translation="LVLDVRGGDEERTVFRFAGAFLAPAEALYARLGRKRSCLQAEEL
                     LILKRHFGISLQALLFRLYHLDIINRSYFDWWYRELSALGWRKREPEEIPRERPQWLR
                     QAVLHAVSEGLLTGRDIRRIFAASPERETVSGFLDRAAFKKQGRAERRRRILEATGGR
                     LQP"
BASE COUNT         5278 a         7579 c         7554 g         4989 t
ORIGIN      
        1 ctttgccgaa agtcggcgac tactcaaaga agacgggatc aattccatcg tctttgccca
       61 caagacaacg gagggttggg aggcccttct ttctggcatg attcgggcca attggacaat
      121 cacagggtcg tggcctatcg ccacagaaag accgggccgt ttgcgatctc aagactcagc
      181 cgctcttgcc accagcgttc accttgtctg ccgtccacgc cccgacgatg ctccagtggg
      241 cgactgggga gaagttctac gtgaacttcc caagcgtgtg ggtaattgga tggagcgact
      301 tcagggcgaa aatatccgtg gcgcggacct ggtcttcgcc tgcatcggcc cggcgctgga
      361 aatcttcagc cgatattccc gggttgaaac tgcggagggt aaggaagtaa aactcgctga
      421 atacttagaa aaaatttggg aggttgtcgg acggaccgcc ttggagaata tcctgggtac
      481 ggcagaagcc aaagcaagaa atggaatggc cggagccctc gaagaggatg cccgcctaac
      541 agcccttttc ctatggactc ttcagagtac gaacggagac gcagaaaaaa tcgagaccga
      601 agcagttacg gataccgatc cgccgacaga agatgacgaa caagattccc aagaatcttc
      661 aaagggcaaa aagaaaaccg gctattcgct gatctacgat gtggtgagac gattcgcaca
      721 gccgctcggt atccacctgg aagaatggga ggaccgtatc atcgtaacgg aaaagggcgt
      781 ggtccgcttg ctgccggctc ttgaacgagc aacactcctc ttcggcgaaa aaggagcaca
      841 attcgctgcc aagcaactgg aaattaaaca aaccgacatt aaccaggtgc tcttgccagg
      901 atttgaacga aaggcaattg caccggagat acgcggacgc cgccgcggtg ggccaaacat
      961 caaagaagat gaggtttctc atcggagagg agcgacaacc ttagaccgtg ttcacgcagc
     1021 catgattctt caatccggcg gacagactaa cgcattgcgg gcactattga aggccgaagt
     1081 tgaacgcggc ccagactttc tgcgattggc aaacgcgctg tcagctcttt accccaagaa
     1141 tagtgaagaa aaacgccttc tcgacgcaat gctgcttgcg attccaagat aatagaaagg
     1201 aaaatgccca tggaagcttg gtacaaaatc gccacaccgc gcaaagaagt ccgtcaaggc
     1261 cgttcgttta atccggatga atttgccatt catcttgaac aggtcattgc cgggaccgcc
     1321 ccagaagact atcgtgatcc aaagcagttt tttgttcgca cctgtttcac acgggcgctc
     1381 cgtgaacacg ctggaatgat acttcggcgg ctgtccggcg agacggcgaa tactgcgccg
     1441 gtcatgacgc tcatcaccca attcggcggc ggcaagaccc atacgcttac cgccctttat
     1501 cacctcgtaa aaaatggaaa taaagcatcc gagtattcag gcgtcggcga cctgttgaag
     1561 gaagcgggta tcagcgcggt tccgaaagcg cgcgtcgccg tattcgtcgg taacgcctgg
     1621 gatcgaaagg aaggacatga gacaccctgg attgacattg cctggcaatt agccggagaa
     1681 aaaggcgtca aggaactcgg tacggcggct aaaaccacgc cgccaggaac agatgctttg
     1741 aaccgggttt ttcaggcggc ggcgagtcca gtactacttc tttttgatga agtgttaaat
     1801 ttcctgaatc gctatcgagg aatggccgat caatttcatt ccttcattca aaatctgacc
     1861 gtggctacga cgggcacgac gtgcggcgca gcggttatta gtttaccgag aagtcaggtc
     1921 gagatgactg attgggatat gcagtggcag gacaagatta ccaaggttgt ccgccgtgtg
     1981 gccaaggacc ttatcgccaa tgatgagacg gaaatcagcg atgtaatacg ccgccgtctt
     2041 tttgaagata ttggaaacga gcggattcgg aagaatgtcg ccaagaccta tgcagattgg
     2101 tgttttgagc gcagagcaca acttcccccg gaatggactg ccgtggatac tacaactact
     2161 gaagtcaagg cgaaagacta tttacgcagc cgatttgaaa tctgttatcc tttccatccg
     2221 gctacgcttt ccgtattcca acgcaaatgg caggtactca cgcaatatca gcagacacgc
     2281 gggacgcttg ccatgcttgc acagtggatt tcatgggcat acaaggaggg ttatcaacag
     2341 gcccgacatg aacctctcat cacgcttggt tcagcgccgt tggaagcgtc tgagttccaa
     2401 agtgtcgttt tgggacaact cggtgagacc cggctcatga ccgcaattga cgcggatatt
     2461 tccggacccc aatcccacgc ccgtgcgctt gacgcagaca ctactggtgc tttgcggaat
     2521 attcaccggc gcgtgggtac ggtaattctc tttgaatctt cgggcgggca ggtagataag
     2581 atggcccatc taccagaact gcggtttgca ctcggcgagc cgagtgtgga tacaacttcc
     2641 gtggataatg ccgcgtttgc tcttgaggac aaatccttct tcatccgcaa ggcaggctcc
     2701 gatggattca ggattggcta tcaacccacc atgaagaagg tggtgagcga ccgccgggcg
     2761 tctcttgacg aagaatctga aatcaagccc gccatgagta aactcgttga agatgaattc
     2821 agacgcggtg caaatattcc aatcgtcgca tttcctgaag acggctctgc tgttcaggat
     2881 acccccaaac tcacgctctt tattatggac ccgcttcatg aattgacggg cgagaataca
     2941 ctgcgcgggc aaattgcgga gtggatacgc atgcgtggca aatcaccacg actttatccg
     3001 ggatcactgg tgtggtgcct gaagaaaccg gggcgtgatc tgcgtgaaag cattgagatg
     3061 tggttggcat ggaagcgaat tacgcgggag attgccgatg gtaccctagg tggcgatttt
     3121 gatcgttctg atcgagctga aattaactcc aagatcgctg cgtctgaaga tgccgctaaa
     3181 gatgacgtgt ggggtggtta ccgtttcgcc gtgattgccg atatgaaaga atccgatggg
     3241 ttgaaagtca ttgatttggg cgcggggcat tcttcaagtg atgaaacact ctgtggccga
     3301 atcatcacgg cactgaggtc ccaggcgttg ttgaatgaat cagtcggagc cggatacatt
     3361 gaccgaaact ggccgccggc attgaaagaa tccggcgcgt ggcccctggc aagtctgcgg
     3421 caaagttttt taaacggatc tctgaccagg ctcattgacc cggatactat tctgcgaagc
     3481 aagattcttg aattcgttga gaaaggtgat tttgggttgg cgtacggaca aaaaccggac
     3541 ggttcattcg agcgtgtctg gttcaaggaa cttatttccg ctgatgaaat atcattcgag
     3601 tcaaatgttt tccttctgac gaagaaaaaa tcccaatctc tcaaggaagg cacacctcca
     3661 ccgccacctc cgcagacccc ggagctaaat cccgaaccgg aaccgggtac aggtccaata
     3721 ccagaaccag gggcgcaaga gaaagccttg cgaatcgttg ggaccgttcc acccgaagtt
     3781 tggaatcgcc ttggcacaaa gatactaccc aagctacgta gcgcaaaaga tctgaaggtt
     3841 ggtgtcgaat tctccgttgt ggtgaccaac gatttaaagg acaacctgat tgcagacctg
     3901 cgacaagcgt tggaggactt agggctttcc gacaaagtcc gaattgaaga ataattgaat
     3961 cctatatggg aggacgttcc ttcaacgggg ccgggacgag tgcctgaatg agaactggtt
     4021 cacgtccctt tacgaggcca gggcgatcat cgaaaactgg aggatggact acaacgaaga
     4081 aagaccgcac agttctctgg gcgatttgac accccgggaa tttgcccggc agacagaaat
     4141 ttcagccgcc tgggagtgct ttgcactcta ctaaatactt gtaaagacta cgagacaaaa
     4201 tacacttcgc actcaataaa ataggaggtt ttgatggata attcctatcc aacacttgag
     4261 ccacttaaat tccttcttca aatgattcac aatcaggaaa tggctcttcc cgattttcaa
     4321 cgggattttg tatgggatcc ttacgcgacg gatgaactta ttgaatctat tattcaaaac
     4381 tttcccgcag gtacgcttct tcgcataaaa aacggaaagg agttactgtt tcagcccaga
     4441 gcttttgagg gagcgcccgt tttatctcca caaaatcaac catcatacct aatcttggac
     4501 ggacaacaaa ggcttacctc actctaccag gccttctatg gatgtggtga acatcgttac
     4561 tatttgaacc tgcaaaaatt agagcaaggt ttggctctcg aagattgtgc gttttacatg
     4621 cggacaaaag atggagaagc aatgtacgga agtcttgaac agcaagctcg cgaactagtg
     4681 tttcctatgc aaaatttatt cggggtttcc ggaggattta gcgcatggca aaatgaagtt
     4741 ttgagggcgt gtagcggaga caagtcctac attttgaatc ttcaggagaa attgtcaaaa
     4801 atccatgatc aatggattca accggtagaa gaatatcggt ttcccatggt tacgcttaac
     4861 gaagaaacgt ccgctcctgc ggtatgcatg atatttgaaa cattaaatag gacggggatt
     4921 aaactcggtg tttttgatct cctaacagcg agattctggc caaaagattt taacatgcga
     4981 atgaagtggg atgaagcaaa agatgcgcat tcgatgattg aagagtataa gatcgacccc
     5041 tattatttgc tgcaaattat tgcaatgttg gaacccggat tagataaaga agggaaagca
     5101 aaggtgccat cgatcaagcg aggagaaata cttaacatga aggtggagca ggctcgacgg
     5161 ggttgggatg ctgctgtgat gggcttaaag aatgcattat taatgcttcg tgatgactgt
     5221 ggcattcttg actcctcgtt gattccctac aatacaataa ttgtaccgat gtcagccata
     5281 ttagccaccc aaacaaatgt aaaagggcct gaatggggtg caaaccgcat taaaattcaa
     5341 aagtggtttt ggtgttcagt atttggtcag cattatgaaa atgcacccaa tagtcaggcc
     5401 gaaaaagatt tcggagaatt aaagcaatgg cttaatggag gcgtcccccc agaatcggtt
     5461 cgtaatttta attttgacat tgatctgcgt caagtaggac caagacaacg cgcagtgtat
     5521 aaaggagtaa tggcccttat tcttaaaaat ggtgccatgg atttttataa acgcgggaag
     5581 ataacatctc aacttttagc tgataagaaa aacccagtgg acgaccatca catatttcct
     5641 cgtgcttttt gtgatacaaa taatatgccc gtatctctac gtgattgcat attaaatcgt
     5701 acttacattg atcgtgaaac caatcattca ttaaataaac gaaaaccttc tgattatttt
     5761 cgtgagatcc gagataaaca aggaaaagca gaggtagacg atctttttaa gtcgcacgca
     5821 atgccaatag gagatacttc tccagtttta tcagataact tcaatgcctt tcttgaattt
     5881 aggcacaaag ctataagtga gatggttcgt caacatacca cctaaattga agaaagaaaa
     5941 tccttacccc ctaaatctgt accaccgtca ggttgaattt tctggccgct gactgcaccc
     6001 ctaaatctgt accactgtta agttgaattc tctggctgcg aactcttcag gcgtacaatt
     6061 accaagggct ccatgcggtc gctttgaatt ataatcgact cgccaatcct cgagtctctg
     6121 ctgcgcggcg gccagggaat aaaaaatctg cgtgttcagg agttcatccc gcatcccgat
     6181 caggaaaatt ttgccataaa ctccattact gttttcttga gcgggggatc caggatttcg
     6241 aatactttac tcgtgatgtg gcacggggac tactgccgca ggagaaactt gccgaggaag
     6301 gcggcccggg cgccactggc ggtgcggctg tcaattcccc ggccgggatc gaagtcgacc
     6361 gtgccggcaa agctgccgcc agcgtacagg ttgcctgagt catcaagcat aacgctgccg
     6421 gtctccttgt cggggccgcc gagggtctgc acccaattaa agctgccgtt ggggtcgaac
     6481 cgactgaaaa aaaggtcctg cttgccgttc gaaacgcgcc tgtcggtacc gttgcccgga
     6541 tcgaaatcca cggtatcgca gaaccagccc caggcgtacg ggttgccggc cctgtcaatc
     6601 gtgacgccgc cctggtcgtc cccgcggccg ccccaggtgc gggcccattg gaagttgcct
     6661 tcggaatcga acttgctgag aaacaggtcc tgcccgccct gggtggagtg gatatccggc
     6721 tttccccgag gattgaaatc gcagttattg ctggtgaaac ggccgcacac gtacacgttc
     6781 ttcatcctgt ccacgcaaac gcttcccatc gcctcttcgc tgcccttgct gccccaggtc
     6841 cggacccatt tgaagcggcc ggcggcgtca aacttgctga gaaaaacatc tacctggatg
     6901 ccgctgtcct gggccgggta tccaagcccg gccttgcccc cggccggatc aaaattgatc
     6961 tcccggctgg catacatgcc ggccacgtac acgttaccct cgccgtcaac cgccaccccg
     7021 ggcccgtcat cataaccttc gccgccccag gtgcgcgccc actgaaaggt gccgtcaaga
     7081 tcgaacttgc tcaggaagac gtcaaagaaa accaggcccg ttgcgggcac gtggttggcg
     7141 tgccaatcgt ggccgcccca gggattgaaa tcaacccggt cgcccgagaa atccccggcg
     7201 acatacaggc agtcgccggc caccgccacg ttatagccct cggcgccgaa ggccgacttg
     7261 ccgggcacta tcttcgggcc ccaggtacgg acccattgga aagagccgtc cggcgcaaac
     7321 ttgctgagaa aaacattatt ttcatagacc tggccgccgg tcgcattgga agtctggatg
     7381 gcggtcttca ctggatcggg gtcaaagtca acggtgctcc ggaaggggcc gaccacatag
     7441 gcatttccaa acctgtccac atccaccccg taggcggtat ccctcttgtt gccgccccag
     7501 gtccgggtcc agagcagctt tcctttggga tcgaacttgc acaggaaggc atcgatggag
     7561 ccggcttccg aggcgcgcca atcaaacgcg gccggatccg ggttgaaatc caccgttccg
     7621 gagaagttcc cggccacgta gaggttcccc tgcttatcgg cggccatttt gttgacctgc
     7681 gcctggtctt tggccgaact gccgccccag gtccttaccc agtcggaaac caggggatcg
     7741 gggctggtat tccggacctc atccgcgatc cccggaaccg gaccgttttt tgagacaagc
     7801 cccaggagca gcaatgctgt tatccccgcg cctatcatca atcgcttcat tttccccacc
     7861 cggttttggt tctcagggcc cggcggataa taaggccggc gagcccggcg atacccagta
     7921 taacgcagat tgtttcgacg tcggaaggat tgacaagccc gtaaaggggg ttggccgacg
     7981 accagggttg caatggttca acgtcattat ggaacaggct gtccagaagt atatgggtgt
     8041 acgccccgat ggccgccccg gcaaaagagg ccgtccaggt agtccgaacc gggacggccg
     8101 gaatcccggt tgcccggttc cactgtttct tgatccactg gcttgccggt tttccgggca
     8161 gaggcattaa aaaagcaata agggtggccc caaggaaggt atgccagaag cggtgcaggt
     8221 atggtttgcc ttgaagaata taaaccaata cttcaatatc aatcgcgatc tgcgccagcc
     8281 cgaaggtggt aaggctgaaa tggcgcggca gggcggcttt cacggccagg cccggtccgg
     8341 catgcaaagg cgtaaacggc attttgccct ttctccgcaa tcggccccgg tcttcagtga
     8401 aggccgaagc cctgggtcaa gcagtcatta cccacccaaa ctctcaaccc caaccggcct
     8461 ctctgaaccg ggcaacctgt cctcttccac gacggatttg atggataatc ccgcccggcc
     8521 attccttctc aaccgttctc ctgcagccag cggtcgagca tccggacctt ctcggcgatc
     8581 atctcggcgg cggccgagta ccggccgtcg aggcggcggt tgaactccag ccagggcgcc
     8641 tcggcgtaca tcggcagggc cgccagggcg ttctcccgct cgtcgagcgc cgccagccgc
     8701 atggcggccg ggtcgccgcc gttgcggccg atgaaccagc gggccgtgtt ccagcaggcc
     8761 cggttggccc ggtaaaccgc ctcggtcagc cgggtctcct cgcccaggcg gctccggtca
     8821 gccccggtcg cgcagagggg cgcggcgcgt ccaaggcaat cgagggcgcg gccggccgcc
     8881 gcggcggcca ggccgtactc gtcgcggacg atttcctccg gactccgcgt ttcaccgggc
     8941 aggggttcgg gcaggccgcc gctggggttg aaatcgtcta tggccaggca ggtccgggtg
     9001 cggctgacgt gctgggcgcc gaaggtctcg ccgtgctcct ggaggtaaaa gaggtagccg
     9061 tcgaagaccg ggctcagttt caggcccttt tcgggcacga gcggatggca ggggcccaaa
     9121 aaggaggggc cgacgtagta gaaattgaac tggagcagcg gcaggcggcc ggccgactcg
     9181 cccaggtgtc tccaggcctc caggaccagc ggcgcggcgg ccgggccgaa gtcgcgctcg
     9241 gccaggcgtt tcaggaaggt cccggccggc aggtcggcgg cgtaggcggc ccagagcccc
     9301 agcgcctcgg cgcgcgagtt gaacatgccg aagaagagcc aggactggtg cacggcaaag
     9361 ggcgacaggc tccgcaccac ctcccacttg gcggccaggc gcggcaaggc cgggaagtag
     9421 gggagctgga tgcactccag gccgatgccg gtctcggtct tgaccaccag cggccgcttc
     9481 acctcgcggc aggcggccgc gcacttcacc atcgggtcgg ccggaccgcc gtaatcaacg
     9541 ctgtaatccc agatgttctt gaggtagccc tccttgcgga aggcctggtc cttctcgacg
     9601 ctgagcatga aggcgacctc cggcggcagg cggcggatga attccagccg gtccggccgc
     9661 tcccagccgg tggtggtgta aggccaggcc gccaggtagg ccgccgggtt cgccccgcgc
     9721 agcgattccc ggatctcgcc caggatcccg gccagcacct cttcggtgct ccgaccctcg
     9781 cagcgcgggc atttctcgcg ctgttgcggc caccacatct tgcagtggta gaaggactcc
     9841 tgggccacga tcaaaatgag cccggccagt tccgggaccg cgccgaagag gcggccgaac
     9901 tgttcccggt agaaggcccg cccctcttcg ctttcggtgc aaaggaagcg gagccggagg
     9961 cccgggtccc gctccaccac gatgccgccg gccgccgggt gggccgcgaa aaccgggtgg
    10021 tcctcccgca gcttggggcc gaccgccacg taactgaagc ggacgccgta ccgggcggcc
    10081 cggcgggccg cctcctgcag gacggccagg ttgcggccgg cctccgggtg gttcagttcg
    10141 ggcaggaccg cgctttcggc gtagcagagc aggtcgccgt agatcatcat ttcgttgacg
    10201 ccgtaatggg cgtaaagacg gaaggcgtcg tcgtccaggt actcggggcc gaagtccgga
    10261 accgagtagt tgccgcccca cggcccctgg ctgatctggt gtccccaggc ggcgcgccct
    10321 tcatgcggac cggccggcag gaaggggccg cggcgcgcgc ccatctcgaa ctccagccag
    10381 gccaccgctg cccagaggcc ggggaccccg ccggcttcaa tcgccaggag gtccggccgg
    10441 gaatcccagc ggaagtcgcg ctcggccagg ccgtccgtag accggcgcag cagaatccgc
    10501 ggccggccgg ccgagccggt ctgcacgccc aggcggccga gaaaatcctc caggtcccgg
    10561 cgcaccagcc cggccacttc cgggtccggc cagtcgcaga agatggccgc gccgtcacca
    10621 acgaccaggt cgcccgacgc cggccgggcc gccgggtcgc gcatcaccag ccggtgcggg
    10681 tcacgccgct ggcggcagag cgggtcggcg ttggacttcc ggagcaggtc gcggatatgg
    10741 agtgaattgt gcatgtcggt tttcccggag atttggctgg aatcgattca gaaaaaaaca
    10801 aagccgaccg ctggaccggc cggccggaaa ccggagccct tcaattgcgg tgccggtcgt
    10861 aaatctcctc gaaggcggcg gcgatcctgg tgaacgcccc caggacgtcc gggtcgaact
    10921 ggtcgggcga ggtcttccgg tcgccctcgg tgatcatctt gaaggcggtc gagtggtcga
    10981 attccggctt gtagggtcgg cggctccgga gcgcgtcgta gatgtcgcag atcatgacga
    11041 tccgggcctc gagcggaatg gctgccccct tgaggccgtg cgggtacccg gacccgtccc
    11101 acttctcgtg gtggcagagg gccaggcggg ccgaaagctg aatggtcggg tagcgggagc
    11161 cggccaggat cttctcgcct atcagggtgt gcgtcttcat gatctcgaac tcttccgggg
    11221 tgaggccgcc cggcttcaac agaatatggt cagggatgcc gatcttcccg atgtcgtgca
    11281 tggcgctggt gaaggttatc tcctcgatga aggcgtggga catgcccatg gcctcggaaa
    11341 ggtctttgac gtaaagacga atccgggtaa gatggcgtcc ggtgtcggaa tcccggtact
    11401 cgccggccaa ggtcaaccgc aggacgatct cgcggctcat gctgtgaagc atgtccaggg
    11461 cattcgagag ctcgccggtc ttctcgtcaa tgatcttctg cagtttccct tcgtactgct
    11521 gttcgacgtc aaagaggcgc tggatatgac tgttgagtcc ctcggccgaa aggaagctcg
    11581 gcagcgtcag gccggtccgg ctgcggggcg gcgtcggcgc ccgggcgccg accagcgaga
    11641 cgatcgaaag gtacttgtcg ctcagcttgc cggccgcctc gtccaggcgg cagacgtggg
    11701 cgaccacgaa gacgaagttg agcgagatga agaaggccgt cggctttatc cagtagcggg
    11761 cgaaagtgaa tagaagcagg gaaccgagga cgatgatggc gcagccggcc acccagagca
    11821 ggcccacctt ccggtaactg ctcttcgcca gcagggtgaa gatcggaaag aagagaaaca
    11881 ggcccaggaa acgccggaac cagtcgccgg ccaccttgag gctgtctcca ttgagcaggt
    11941 tgtccagcat ggtggcgtgc acctcgaccc cggccatccg gttgcggtgc tggctgaagg
    12001 gcgtggtgaa aacgtccccc agcccgggcg cgctcgcgcc cagcaggacg gccttgcccc
    12061 ggaagaatga agacgggtaa tcccccagga tgacatttcc catcgaaagg tagggatagg
    12121 tccggggcgg tccgcggaaa ttgatccggg tcgggtccac ctggaatatg acgttccggc
    12181 ccggctcggc cttgaccgcc gccgaggggc ttccccggcg catgaccttg tcggtcatgg
    12241 tctcgtagag ggccgaggag aaggagggaa tccccttctg ctggtagtag agggtgtgga
    12301 aggattcccg caggatggcg tccaccgccg gttcgacgtg aatgtggccg gtccgatggg
    12361 gcttgaaggc cgacagcggg tagtggggcg cggcctcgga gtcgaagtag acgggcagga
    12421 tcaccctccc ctgctgccgg atggccaggt tgaagatgga gtcatccgcc gagggttcgt
    12481 ccatgatgac gtcgaagccc accgcgtccg cctcccggac cgactccagg aaggcggcgt
    12541 agttgcggcg cggaaagggc cagcggccca gggcgtccag cgtctcttcg tcgatgtcta
    12601 cgatgacgat ccgcgggtcc ggatggcgcg ccccccggag gcggaaggag agatcatagg
    12661 agtaattgtc cgtcccgtcg aaaaggccga gatagtcgaa aaagaatatc aggcacaggc
    12721 cggccaggac gaagacggcc aggcgccggc cggccggaaa caatttcatg cttcgcgttt
    12781 tcttccgggt tcagggcacc aggatcgtga agatgatggc ggtaagcgtc acccagaacc
    12841 cagcgttgga cgacggcttg acgtcgaagc tctggggagc cgagaagtcc ccctcctggt
    12901 tccaccggtc gatggcggcg gtgcgggcgt agtaggtgcc gctcttcttg ggcttctcca
    12961 gggtgatctc cgaggccgga accttctggt cgaccaggac cttgccaaac tccggttcgc
    13021 cggccagctg gaaccggtaa ctggtcccat cgcccaggtc ctgccagcga atggaaatgt
    13081 ccgttttgct gatcgtggga gcctcgaccg ccggcgcgtc cttgggcggc ggcagctgca
    13141 tcgcgaagcg cagcgggtcg gaccaggcgc cctgccggtt gtcgacatca atcgaactca
    13201 cccggaagta gtagctctta aactcgagat cgccggtctc gtaggagttg gcggtgatgt
    13261 cactgctctc cttcaccagc ttggtgaacc cggcgtcgtc ggcgatctgg acatggtagc
    13321 gcgaggcgtc ggccaccttg agccacttgc aggtcagcgc ctggccgcgg taaacggcct
    13381 ggtcacgcgg cacctcggtg aagggcgtcg ccggcagcgg tttctgcagt tcgaagcgca
    13441 gcgtgtcgga ccagaggccc tgccagttgt cggccgcgac cgcgcttacc cggaagtagt
    13501 aggtgttgta ggcaaggtcg ccgaccggat actcggtccg ggtgaggtcg ctggtgtccc
    13561 tggccatccg ggtgaaagcg gcgtcctcgg cgatctggac atggtagcgc gaggcgtcgg
    13621 ccaccttgag ccacttgcag gtcaggtctt tcccgtaata gacggcctgg tcgcgcggca
    13681 actcggtgaa gggcgcggct gggttggtcc ggacccggag ctccaccggc tccgaagcca
    13741 gcccctcgaa gccggccgcg tcgaggctgg aggcctgcag gtagtaagtc ccgtccttga
    13801 ggccggccag ctcgaagttg tcctggggat tgatcaaccg ctgctcgatc ggctcccgga
    13861 aatcccggtc acgcgccagc acgacccggt aggagacggc gccggaaatt ttctcaaagg
    13921 cgaggcggac cgggaggcgc tgataaacgg caagcggctg gatcaggacg ggagcggccg
    13981 gcaattttac cggcgcggtg ggcgcggttc ccttggccac cagcgtgcct tcgccattct
    14041 tgatctcgac ggtgcccagc tcggccgtga cctcgaccgg atcgtcaaag gcctcgaaac
    14101 gaaccacccc gtccgggccg accgtgctcc ggaaggtggt gccccgcacg ccggcgatcg
    14161 cggtcggcgt ctcgacctga aagcgggtct ccttgccggt ggccttctgg atccgggtga
    14221 ccgtcgatcc cgcgctcagc aggaaacggt aactggagga gacgctcgag gtgcgggcct
    14281 tcttcacctg gacggtggtg gccggccgct ggaccacggt atggccgtcc tggtactggt
    14341 actcgaccag gctgtcggcc ccggtccgga cgatgtcgcc ctcgccgatc ttgtcctcca
    14401 tggtaaccgg acgccaggtc ttggtggcct ccgagtagat ctccgccttg cccctgacaa
    14461 aacgcaggga ggccgaaacc ggttcgccct tgagcaggtc ggtcgggatg atgatgatgt
    14521 cgtccgggta gatcaggttg gggtttttca tgctgttgaa ccgggcgact tcccgccatt
    14581 tcttcggcag ttccaggacc ttgcggcaga tgccgatcag cgtgtcatgc ttgacgaccg
    14641 tgaccttgaa gaggggtttt tcttcctgtg cggcgaccgt accgaggaga gcgaatgaga
    14701 agagaaccag ccaggcgccc aataacatct tgcgaaacat agccgcagcc tccttcctta
    14761 tcatggcccg ccggggcgat atccccggct gaaagattgc cggcccggag caaccgaccc
    14821 cggccgctgg tacacattat atcaccaaaa tcaagggtcg caccgccccc gcctttccgg
    14881 gggagaagaa cgcccctttt tgcctttgta aacttagtac caaaacgggg cggccaggtt
    14941 cactcccggg ccgaccggtt caaggcggcc cggagggaaa tccagccggg caggaaacgg
    15001 tccatcagcc ccttgaagcg gcggttgtgg gaacattcca gcaggtggac catttcgtgc
    15061 acgacgatgt attcgaggta aaccagcggc ttcctggcca gttcggtgtt gatccggatg
    15121 cttccgacgg cggcgttgca ggttccccag cggctcttca tggcgcgcag gaagagcctg
    15181 gaaaccttga cgcccaggcg cgcctcccac ttcgcgatca gcggttcggc cgccagccgc
    15241 agccggccgc ggtaccagtc gtccaggacc ttccgccgtt tcgcgacccc ggcgccgggc
    15301 cgcaccctca gtatcagccg gcccccggcc atcgtcacgc cggccggagc ctccgtctcg
    15361 accacctcaa ggcggtagcg gtcgccccag aggcaatgga tttcaccgtc caggcagcgg
    15421 agcggaagct cggccggccg ctgccgggcc cgttcctgct ggcggcgtat ccagtccagc
    15481 cgggaccggg cgaaggcgcg gacatgctcc aggctcatcc gcgccggggc cgagatgcgc
    15541 acccggccca gcggcgaatc cacgcgcaga taaaggttcc ggatccgctt gcgcgaaatc
    15601 tcgatctcga tgccttccag gtcgatccgc tggatcgccg gcggcgtttc ttttcggttc
    15661 ggcatcatga cttgaaattt gacccgcgtt cagggagagt accgatagaa tataccattt
    15721 ctggtggaaa tttttatcaa tccgtggtaa aagataagcg gcggtccgga agaatcgaaa
    15781 ccggggaggg aaagcatgcc aagcctggta aaagaattcc tggggcaaaa aaacttcgcc
    15841 gtggtgggct ccttccggga gaagacccgc tacgcctacc tgatcctgaa ggacctgctg
    15901 gccagggggt acgaggtcta cccggtgaac ccgaacctgg acgaggtcga ggggcgcccc
    15961 tgctacaagc ggatcagcga catccccttc aggattgacg tggttgacct cgttaccccg
    16021 ccgccggtca ccgagatcct gctggaggag tgccggcagc tgggcatcag gcgggtctgg
    16081 ctgcagcccg gcgccgagag cgagaaggcc atcgaattct gccaccaaaa cggcattaag
    16141 gtgatccacg gcctctgcgt gatgctggag gcgggcaaca agaaggaaga ccttcccccg
    16201 ccgcaatttt agcggccggg aaccccggcc cggagtttcc atccaacccg cgcaatcatt
    16261 cgccccgtct tttcaagtca atcaccgccg gcccgattaa ccggccgccg gccttcaatg
    16321 gtaaaatatc gctggagggt taggctaacg gtaagtcagc ggacttgaaa tccgccggct
    16381 ccgaaaggag ccttgggggt tcaaatccct caccctccgc agcgcctcga aaaacgaggc
    16441 gcgtttttgc gcttggaggg tgagggattt gaagagagcc gtgaagtaga gtccctcggc
    16501 aagctcggaa cacacttcac ggccatgaag cgtgcttcgc tctgcttcat ggcgattatt
    16561 gtttctgaat cttattcaga caaaccagcg ggttccacct acgcttgaaa aacaggcttc
    16621 ggcggataaa ggccgacttt acctaccgta gctttagcgt aggcaggctg aggcgcagtg
    16681 ggagccgagc ggcgccaaat ccccgcaaaa ttttgcgggc aaaattttgc agagaatcga
    16741 aaatcgaaag tcgaaaattg atgcccgcta agactaaaga ttttcgattc ggtctattct
    16801 cgattctcga ttctcaagag gtgggggcgg cggaatcgta agccgcccaa aggtggccga
    16861 ttgcagacaa aaccgccgtt ttttgcccgt catccccgaa tgtggtcgtc ggggatccag
    16921 accgcctgac agacccgcaa agaaaaaacc tggattcccg ccaactatcc gcgggaatga
    16981 catgctttta cggattcccg cctacgacct tcggagacgg acacgcgggg attccgggca
    17041 cctaccgcag taatatttcc cgcaccaggg ccacgttctc ctcgaccatg tcgtcgcggt
    17101 cgccgcggaa cattccgaca ttcaccacgt cgcccggctt gatgtggcgc agggcatatt
    17161 cgaacgacat cctggggtcc atacgcccgg cgcccatgat cttgtagccg atgcagggtt
    17221 tcttgatcgc ccgtatgcac tcgaccgccg gaccgatgtc gtccaggctg aacttgtcgc
    17281 cattgccgtc atgcaggctg ccgcagttga agaaacagac ggcgtggaag tcaaccaggt
    17341 cgagaccggc tacccagcga tgcacctcgg gcgcatggcc ggccacgccc accggtacgc
    17401 cgtgcgaacg ggcgtgggcg caccaggtcc gcagcgccga ctcatcccgc gcggagtaga
    17461 cgcggtccat gactccgccg tgaaaataga gggcccggca gccaatggcc accgccttgt
    17521 cgatcgactg gagcatggcc tcggtatccc ggccgttgag ctgggcgatc cactggagct
    17581 tgccgccttc cgcccggtat tcgcggaccg cctgaaccac gtggggggtc tcattgttgg
    17641 tcaccatcgc ggtgatgccg gccgcctcgg cccggcgcca ggtctccttg atccgggcaa
    17701 ccgtatagta atcaaccatc gcccggtccc gttccgggct ctggtggctg taaccgccga
    17761 acgggttggc gcccagcacc agccgggata tcctcaaacc gcagaaatca acctccggca
    17821 accgattatc catgttttta ttgtaacaca gccggcccgg ccaccgaacc gggcttccct
    17881 tcaaagacgg gactgaaggg tgatcggggt gccccgccag aaggagaagt tcacttcgat
    17941 gaagctgaac caccggtaat cgacacgctc tccctgccgg atctccacca gaaagaagca
    18001 gagggcctga aacggatcgg ccccgagaag attcgggccg agcgatagtt ttcccgcgtc
    18061 gtccgtcttg ccctggtgcc ggggcgtccc gtcgagcgcc tggcggtagt agttggtgcg
    18121 ccgggaccgg tagactttca cctcggcctg gcgcaggggg gccccgtctt tgccaagaac
    18181 ctgcagcacg gaatcgcgcg gctggtagtc gatccaggag tgattgacga ccggcagcgt
    18241 tgcgatctcc cggttgataa tctccgcgtc ccaggcgtga aagggaccca ggtcatccat
    18301 catcgtgccg cgtacctggc cgtagttgtc ggaaggaatc ggctcatggt tgacggcgtt
    18361 gtccttcgcc tcgattccga gcgcgtagaa atcgggcaat ccgagcatgt ggcccagctc
    18421 atggcagaga accagggccg agcgctcgac gaacatgtcc tttccggtgc cggaatcgcc
    18481 cctggccccg tggacctcga cggatgagca ccaggggcac ggccagttgg cgcccggggc
    18541 gttctcgtcc atcgccacca ggatgtagtc gtgcgggtgc gtctcgtaat cggccgggag
    18601 actcaggccg ccggaattcg ccggctgccg gacgccggtc ttctcctgct tgtcataggt
    18661 gcgcaccccg gccaccgaga aacggcgccg gatgcccccg gcctcgaaag aagcgttgat
    18721 ccgttccacc atgctcttga tacgttcggc cgcgtcggga tggcgcttga gcgtctgggc
    18781 ttcgacggca aagaccagcg cgtgattgat gatcgcggcc tgaggctgac ggcgtccgat
    18841 ccggtccagc tgttcgccgg tcaaaacggc ggccatcgcc tgccaggcgg ccgggttggc
    18901 cccggcttga taggccgcct ggatcgcacg cctctgctct tcatttacga cggcctcatc
    18961 cagaatcctg gccaggtggg gggaggtgtc cccggcagtc tgtccgccca ggcgcgcgaa
    19021 cggcacccag gcccccgtcg tgaacctggc gttcctggga acgggcggca tgacattatt
    19081 acccgccttc gtccccggtc cggaggaagt agccggggcg gcggccgccg ggtcgctctt
    19141 cggcggggcc ggcggcggag agctcctttc cccggccggc ggcgggacaa ccggggcggc
    19201 ttgtctcaat ttggagaaga cctcgcggaa ccgggaggtc gtctgggggc cggaaaacaa
    19261 ccagacggcc tccgcctcgc cggcgatcag gccaatgtgc tcaataacct tgtcaaccgg
    19321 gatcaggggg ccattctcgc ggtcctgccg atacctcagc atgatctgaa catgcacctt
    19381 gacatccggg ttggctttcc ggagacgcct cacaacatcg aggaccttct ggacctcaaa
    19441 accggacgat ttctgcaagg cctgcgtctg aacgacaacc atgtccgccc cgccggccac
    19501 cgcctctccc ccggtttcca tcaggcggcc cattaaaggt ccgaaaacgt aaaccaggcc
    19561 ggccgcccgg gccaacccgg caacctggcg ctcccgttcg accagcttct cggcatgaaa
    19621 agaattctca aggttatagc cgagcatcgt gacgccgtga cgccgggcct gggccaggag
    19681 cgccgccatc tcgtcatcct tcatggcctg ccacctgacc tccggtgtct tctcatgctt
    19741 cggcccgatc aggagcagtt tccggccaac ggtcaccccg tccagcaggg ccaggttttc
    19801 cggccggacg gcgacgatat cggtagggcg cagtaacggg ttcaggaact cgacgtcgga
    19861 ggcatccagg atgatgtagg ccagggggat ggtggaaatc gtttcgcact gaacggattc
    19921 aatcgaaccg gccagaaccg tcagggcggc cagaataaaa gccccagcca tccgaatctt
    19981 tcccattttg cttcctccct gggcggccgg cgcccgacct gaacgggggg cggcggctaa
    20041 ggtggccggc accagccggg atatcctgcc cctattttaa catggacggc cggttcaagc
    20101 gacgcctctt tttctgccgg tcaatccgtg tgttatactt gaaaccgacc gtttttgcct
    20161 gaaaatcaag aatggcctct tctcattcat caataggccg acccgggcgc cggcggagga
    20221 aagatgaaga ataaccacaa agggttgcaa ttaaccgcct ggatgctgtc aatgctcggc
    20281 ctcttctggc tggcgggcca gccgccggcg gccgcccagg agaacaagaa gggaatcgac
    20341 atgaaaaaag gggaacgggt tttgttcttc gacgaccggt acgtcgagga gatgaaggaa
    20401 ctcacccggg tgatgcatcc ggccgtcaag cacgcgggca acccggtcct caaggccgac
    20461 aagccctggg aggtgagcga ccggaaggcc ggcatcggga tcgtccacgt ttacgggacg
    20521 gtgcgcaagg agggcgacct cttcaagatc tggtaccagg tctatccccg cagcggaaaa
    20581 ctttactggg tctgctacgc cacctccaaa gacggcatcc actgggagaa gccggccctg
    20641 gacctcatcg agtacaaccg gctcaagcgg ggcgaattta ccgaggagga agtcaaggga
    20701 aacaacatcg tcctgatcaa ctgcggccgg ccgggcgcca ccatggcctg ctcgccgacc
    20761 atcagcgtcg acccggacga acccgacccg aaccggcgct acaagatgct cttctgggac
    20821 ttcggggtcc gctactatga accgggccag tacatgggcg ggtgcgtggc cttttcgccg
    20881 gacggcatcc gctggacgcc ctaccccggc aacccggtcc tggtcgccag cgacgtgacg
    20941 gacgccacct gggacccggt cgagcgccgc tacatcgccc acttcaagct ttaccagctg
    21001 ctgacggagg cggccgaccc ggccaggccg gtcgagatgg agaagccggc cgaattcttc
    21061 ctggtcggcc gcaaaaagcc aacccaggaa aaggacacca tcctggccga ggcctacttc
    21121 ctggacacga cgctacagca ggtcacactt gaaacccgga cgctgcgcaa ctgccggaga
    21181 atcagcgacc gacgcatcgt ggcccgggcc gaaagcaagg atttcatcaa ctggaccaac
    21241 taccgggcgg ccatcatccc ggaccggctg gacgcggcca accagcagac ctacggcatg
    21301 agcgccttcc ggtacgaggg gctctacgtc gggctgctgc ggatcatgac cggtcagtcc
    21361 atcgatatcc agctggccta cagcaacgac ggggcggact ggcagcgctg cttcgaccgg
    21421 acctccttca taccctgcgg ggccgccggc ggctgggacg ccggcatggt cctgaccgcc
    21481 agcgagccgg tgctggtcgg cgaggagctc tggttctact acggcggctg gcagaagcag
    21541 caccacgagt ggggcgagac ctcgatcggg atcgccaaac tccggcggga cgggttcgtt
    21601 tcgctggacg ccggcgagaa ggagggtgaa ctgctcaccc ggccggtgac ggtgtccggg
    21661 ccgcgcctga aggtcaacgc ggccgccgcc ggcggagcgg tcgccgccga ggtcctggac
    21721 gaatcgggcc gggtcatccc cggctacgga aggaaggagt gccggccggt cgcggccgac
    21781 acgaccgccg gagaattaac ctgggcggga aatcggaacc tgtcggccct ggccggccgg
    21841 aaggtcaggg tccgcttctt cctgaagcag gcccacctct actccttctg gcagggggag
    21901 taaagggtct tgactatttt gccgttttta aagaggtact cggacccgaa attcttgacg
    21961 atgccgttca agcggccgtt cctgaagtgc gacgaccgga ccagttctcc ctcgacataa
    22021 tatttgcaca agccgtccag ccgtccgttt ttatagcgca gctggcttac gagaaggcgc
    22081 tttttaccga ggtactcgta acgcttgcag agaccgtgca gccggccgtc cttgtagcgc
    22141 gagaccgagg cgagccggcc tctcccgctg caatatgccg gcccgtcact atcgtaatat
    22201 tcccggacca ggccgttccg ttccttttcc gggggcgctt cttccgccgg cccggcctcc
    22261 gcaaccggtt ccccgaccgc ttcttcggcc gggggtacct gcggcccctt cacgccgctc
    22321 ccgccctccc agagaggccg gttcgcctcg acccaggcgt aatacctctt tccctccggg
    22381 gagtcgtcgg taaccgggaa tccggccagg taaccgaagg cgtattcggc gaggctcatt
    22441 ttcatgccgg cctcatcttc caggttttgg gtgattcctt ccagggccct tttgtccttc
    22501 tcgaggctgc ccaggcagaa acgcccggaa agcagcttca ggagcctggc gaaaggggaa
    22561 agaccttgaa tgaaatcgtc gaaaggcgtc atgtattcgg ttaacccttc gacgtgttgc
    22621 cttgcctcgg cgagcgaaaa atcatccccc cactccaaga ggtcgaattg cacggtttcg
    22681 ccggccgccg cccccgacaa cgccagctca aaaaaacgca ggggggcgag cagggtctcg
    22741 gcataggcgt cgcctcttat ccgccgcagt atcttcctta cccggccccg cgccttcccg
    22801 gcgcttgtct cgacgctggc aaaaaaatcc ccctcctggt cctggtcgat aaagacgtcg
    22861 gcggaggaga agagggcgaa ccagagcggc ggcgcgtacg gagtaagaaa atcgtcggat
    22921 agcttgtagc caaggctgaa cgggctcagg tatgtccagg ttcccatcag gccctccttg
    22981 tggttgaagc cggccgcccg tcgcctcgag gatgcggcgg cggcgttccg cgcgtccctg
    23041 ttttttgaat tcgttatcgc tttcccgaat tccgcccggc cggcttcttc cttttttgtc
    23101 tcttccgagg cgcccgcaat aaatccggct cttttttcga catcagccct atggatgtcc
    23161 cttccatcgc ttcctgttcc tgcctggtcg cttttctcat ttgagcctcc gttgatatgc
    23221 cgcccccacc ccgacaacgc ttaaaaagag aagcccttcc ggctggcctg ctcgcgcacc
    23281 acccgcgcat ccttgaaccg gccgagtttt tccagcgtaa gcactttgtt gtgccaggcg
    23341 tgaacgttct ttgggttcaa tttaagagcc ttttcaaaaa tcggcagcgc ctccttgaac
    23401 cgaccgagcc cataaagcgc ccgccccttg ctgtgccaaa catcctcgtc tttcggatca
    23461 agttcaagcg accgttcaaa ggccgccagg gccgccttta accggccgct ccggccaagc
    23521 aggcgcccct tgttgttcca ggccgcggca tcctttggat tcaactttac taccttctca
    23581 taggccgtca gcgcttcctt aaaccggcgg cggctgtaaa gcagatatcc tttctggtac
    23641 caggtatcgg catgcttcgg atccagctcg agcgccttct cataggcggc cagcgccgcc
    23701 ttaaaccggc caagctcatg aagcacaagt cctttgctat gccaggccgc gccatttttt
    23761 ggatccagct taagcgcctt ctcgtaggcc gccaacgact cattaaaccg gcccagattg
    23821 taaagcgcgt cccctttctt cttccaggta tcggcctgct tcggatccag atcgagtgac
    23881 ttctcccaag cggccagggc cgctttaaac cggtccagat tgtaaagcgc atcaccttta
    23941 ttcttccagc tattggcctg ctccgggtcc agttcgagcg ccttattcca ggcggccagc
    24001 gactccttaa accggccgag attttcaagg atggccccct tgataaccca ggtcaaaata
    24061 ttttccgaat ccagtttgat cgccttgttg caggccgcca gggcctcttt aaaacggccc
    24121 agatcacaaa gcacgacccc ctttttcctc caagcatcgg catccttcgg attcagctga
    24181 atcgactttt caagggccgc cagggcctcg ttgaaccggc cgagccggcc aaataaacat
    24241 cccttgttat accagatcgc atcatccttc ggatccagtt cgagcgccgt atcccaagcg
    24301 gccagcgact cattaagccg gtccagattg taaagcgcat cacctttctt acgccaggca
    24361 ttgacctgct tagggttcag ctcgagcgtc ttttcgtagg cggccagcgt ctccttaaac
    24421 cggcccagat tgtaaagcgc gtttcctttc ttctcccagg tatcggcatc ctccggatcc
    24481 agctcgagcg ccttctcata ggcggccaaa gcctcgttaa accgttccag actgtcgagc
    24541 gcaaacccct tgacaataaa aatgatgccc ttatcaggac gcaacctgga tgccttatcg
    24601 cagacctcca gcgcctcatg aaaccgatcg agccggcaaa gcacaagccc tttattatac
    24661 aaggcctcga tatcgtcggg atccagttca agcgtctttt cacaggacgc cagcgcctct
    24721 tcaaatttct cctcaagata ggcctttttg cctgcctggt tcaataatgc gattgccttt
    24781 tcctggtcca tttttcagct ccttgaaatc atgaaattta agggcgcttc agcggccccg
    24841 gttcaatgcg aagccgttgg catacaggtc gggctcaggt atgtccaggt tcccatcagg
    24901 ccctccttat ggttgaagcc ggccgcccgt cgcctcgagg atgcggcggc ggcgttccgc
    24961 gcgtccctgt tttttgaagg cggcccggtc gaggaagccg gagaccgtct cccgttccgg
    25021 ggaggcggcg aagatccggc ggatgtcgcg gccggtgagc aggccttccg aaacggcgtg
    25081 caagaccgcc tggcgcagcc actggggccg ctcccggggg atttcttccg gctcgcgttt
    25141 ccgccagccc agggcgctga gttcccggta ccaccagtcg aaataagacc ggttgatgat
    25201 gtccaggtgg tagaggcgga acaagagggc ctggaggctg attccaaagt ggcgctttag
    25261 gatgagcagt tcctcggcct ggaggcagga gcgtttccgg cccagcctgg cgtaaagcgc
    25321 ctcggccggc gcgagaaagg ccccggcgaa gcggaagacc gtccgctcct cgtcgccgcc
    25381 gcgcacatcc agcacgaggt
//
LOCUS       DAOQYN010000003        23770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_2483_length_23770_cov_4.797723, whole genome shotgun sequence.
ACCESSION   DAOQYN010000003 DAOQYN010000000
VERSION     DAOQYN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23770
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2483_length_23770_cov_4.797723"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="PKN80_00260"
     CDS             <1..1433
                     /locus_tag="PKN80_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ34484.1"
                     /translation="PWYPEVVDQVTEAVKSMAWAGGEGFSLYDGRFPGGDFTQLDAYK
                     CLFEYDGSLKPSGVVLAVMANLLDGFRGVGQPVINPVLDTFELEAPARFAFAVCGADR
                     RVLEGSVRLPEGVRAYDFMGNQLDPAAPLLFPNYGLLYLVGPRERLEATRAALAAAEL
                     NPPVELKLEQVLDEKSGQYRVRATVTNRRPDRPLTGKILFDAPQLRDFREKVQPVVLK
                     PGESRDIYFGLNYYRGDRFEPPATRLNLYFDGVRSDQTLSGFTETHSLRLRKADALLK
                     EKDFAGAEAIYRELLPVMSVGQASETGLKIAQALQGQEKSEAALAEFRRVAEGAGGRP
                     QDRAAAWLALAENHEGRQEIDAAAAAYRKAGELEGGPAGTAATAWYQMARMLRARNQA
                     SSRDAETALEAYRKSAAIPEAWANIGGCAQLYLAHFLRDLKRYPEARVEYRKLLDKPG
                     ALAMYVKQAREALEALEKSLAAPAAR"
     gene            complement(1521..2456)
                     /locus_tag="PKN80_00265"
     CDS             complement(1521..2456)
                     /locus_tag="PKN80_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34485.1"
                     /translation="MEFIFYRYNHAFYVLKPDAIRDDYREIKDLGGDAVGFAATIEDV
                     GAVPRSILKHIELMRETGLRPYFTFGRWGGLFAAAAVNTSSRRTFQHRDWWKRDPEGN
                     PPGALVCCVNNPGFREYFFEVAERAFAKFRPDGLIMDEPKDSNWPCYCPHCRKLGDPA
                     EIHIPTLAKFLGEVCTLFKRTVPGGATYLFHMPWQEPSFYEATAREADLDYLGVDGPT
                     CRQVKENGADIGKKPLLESIEVIYPVAERFGKKKLVLPENFDVPAGEEENYYRNARKV
                     LQTRPDAVIFHHYGFENEDPDLIMKHIARLIGEFK"
     gene            complement(2501..5059)
                     /locus_tag="PKN80_00270"
     CDS             complement(2501..5059)
                     /locus_tag="PKN80_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34486.1"
                     /translation="MNRRQRISFLLLTVLLLGGLLLRPDRLGAQVRAESVAFPGYEEM
                     DGAYHGLLAAANGRVYLGLCTHNGRDDAVLLEYDPTGGRTRLAADLGRATGEKGRGRL
                     PQGKLHTRIREGRDGRFYFATHFSYPLNDLKAKIDYPGGAFLSYDPKTGRCRVLGRAP
                     AGEGIISAELAPEQELMYGLTYPSGLFLVCDLRRGRVSNLGPATDGKTVCRSLGCDSD
                     GNAYFSREPDLIVRYDRRARRLQVLPTRVPSLDLEAPEWAGNTVGRRIWRTVVWDRTA
                     GLFYGVHGGTSKLFSFHPASGQVKDLGQVCSREEIGREKLTYATLALAQAPDATLYYV
                     PARGLFDYFNSQPLNGPAHLVVRRPGDGQPQDLGPILCEDGRRLFGSQSAAVSPDGRT
                     LYLLGAVEAKTGDRSVPFTTLPVAGADGPARVPYQLRLLKIDLADLPAGPDLAVLPAG
                     PAAAARQPGRVNAGWFHSFSAEYPYLDGQWHGIITASDGRTYFAVASHEKGHHTQFFV
                     FDPKLKKVTHLADLGEVCGETNSGRTAQGKIHSQISEHKGRLYFTTLHADYTAAGEAD
                     YPGGHIISYDLKSGRFTDFGIPRPGEGLETAALDPGREVFYALSWPGDHGQGPNHLVG
                     LDLKSGRTRDLGIAEPPGPVSRMLLVDGRGVVYSARSGGRLWRFDPASGRIEELDLRL
                     PEAPYAPAFKPDDKNGRSFVIRSGFWAAAEKCFYLLVHGTELLCRFHPESGRFEALGD
                     FGLRKNYPRPAALGFCRSGRRIYYTAWGEGRFTNLVSYDLDKGTFTDHGTIYAEGRRA
                     AEIHALSAGPDGKIYAVAALYNAAGDPVIKRSFTKGGQAYQMAFLVIDPERDFQ"
     gene            complement(5120..6823)
                     /locus_tag="PKN80_00275"
     CDS             complement(5120..6823)
                     /locus_tag="PKN80_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNQ34487.1"
                     /translation="MLGLSQADWLVMIIYLAATTYFGVMAGRGLSGIADFFMPRRFGK
                     AMMLMHTFGTGTHSDQAVNVAAKTFTNGVSGIWYQWLWLFVSPFYWLIAPVMRRFRAV
                     TTADVFEYRFNRSVAMLFSVVGILQLMVAMGVMLKGSGKVIVACTGNLLSADWAIWAM
                     TVIVVIYGMAGGLAAAIITDFFQGILTIVFSFMLMPLVLHATGGIAGLKASITDPAMF
                     SLVAPAEIGLFYIIIISLNGLVGIVAQPHTMGNCAAGRTEMDGRVGFMFGSFIKRICT
                     VGWTLTGLAAVAYFAGQAMDPEQVFGAVAREFLPRVSHGMLGLFLAALLASVMSSCDS
                     FMIASAALFTENIYRPLRPNRDQKHYLAIGRWATLLVVVGGLIFAYLFPGVLKGLEIF
                     WMIPPMMGIAFWLGLFWRGMTPAGAWASTLAGFAVWWLTTRAWFVAFLAGLSGLEGLK
                     LVILKGGRPEIYLPWQMVFSLAGGVLAGLLVSLFTRNIPKERLDNFYALVATPVQPGE
                     QTEKPCTLPAGTVPVKRRRLFPGTELEILVPSRTSVLGFLAGLLVAILNIGFVFWLLR
                     R"
     gene            complement(6862..10125)
                     /locus_tag="PKN80_00280"
     CDS             complement(6862..10125)
                     /locus_tag="PKN80_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34488.1"
                     /translation="MRYRMIAAGLLAALTLTAAAAGRAQTRPAAEEYHWGRDAAYFDR
                     LDGFGRALLGSGRSYAGTRQTGSSIQARLDRELPPGSYRVLVQSWNVDTGLNQFEVDL
                     NGAVARLKMDGARFNREAMTAVLTTTRPGDLLTITAREIGQRELRLYWVWLSSNLGDQ
                     GEFRRGVALGPLPGSGGEPAGNLLPGSSFEAGLNDLWGKAYASRGFIIDDYLEAAVDA
                     PHGRFCLRLPDIGPVRLVNRPVPVTRGGRFTLSGYFRTLKPAAVQLTATALRDLESRP
                     AVFTAPLETGPEWKRTSTSFELPTDFTGTIQVSVLWNDKQPDPAAAPTDIWTDAIQLE
                     RGELTAYRSAYESEAGLVITGKPGAVFYTGEPVGVEVAAAGTPAEIELKILDLNLQPV
                     LEKRLNIAVEKGRGGQLVRLELSQPGAYLAVVRSLAPGAAPAVRKAFALIEPPPDRPR
                     ADLRTGLYATLSPESVPIFHRLGFRYLNTLSAMAGIGRMSLAAPERDRYLWYDWAIDL
                     ANQNGVEVVVCLGNRYPAWALEPGKTYPSIAAWTEFVARMVEHYRGRVHYWMVEDEPG
                     GRVPADHYQKMVAAARQAVQKIDPAASVVMHGDSVKFFGQVMAAAGGKHDFYDILAGG
                     ANLAANTKPQIEAARAAGKEVWAITFGGSKDSLYTEQPTGATAGVLAKFFVERLHELD
                     VSRFFHYDARIMGMEPGTYWSRSSLFEFDGAFRPAGIAYAAAGNLLAGSRPLAGATPF
                     QGLQVYQFERDGGRLAALWTDGRILKGRLTKTDGLTRRDTTGAVQPLSANGEIILDSN
                     PRYLAGGPDLAERLAGLRLEPALDFVTYVEETPGSRAADFVIRLKNNLSTPLELTALP
                     QLGTASWFAVAGNRRRFDLPVNLTLKPGETGRIAFPCLSHPEIPLPPGAAMNLELVSR
                     LFSTRLPIDLAGYRANRVAEGGFKNFPSIHGAGYPMLTLGEGRKLAVSRDGENLYLDL
                     HLLKSSADPRASLEVRFIPDLARQPWGETLQPLLRLQAGDGSAELTGLAAGQAPPPLK
                     GVRSERPEDLFIHLALPLKTLGLKTGQALGLRAELQETDGTAWRLEGPGGVRAISIW"
     gene            complement(10209..12125)
                     /locus_tag="PKN80_00285"
     CDS             complement(10209..12125)
                     /locus_tag="PKN80_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34489.1"
                     /translation="MEKLEKNEFTLRSIITGAVMAALIALGNVYNQIAVQGSYMAEDF
                     TASSAIFFLFWLVLLNLLLRRYCRRLAFSSAEMILVYVMMIVACAIPTMGLILYLIPT
                     ISSASYYATSSNRWAETIIPYLKPWLIVRDENAVAWFYEGLPKGESIPWLAWVKPLLP
                     WAAFLMALYLVMIFLMVILRRQWEQNEILNYPLTKTPLEMINAHANNLFRNPLFWTGF
                     AITALIGSLNGLHFYFESVPALKLDNTIPIFRATIQLPFRVSFPMIGFTYFVNIPMSF
                     SLWFFALLTTVEQGFLNITGFGTTGYLPYTARPILDWQSLGALVALMLYSFWLARRQL
                     ASVFTAAVRPKANPDDADEIVPYRVAFWGTIICLVFLVGWLVASGLPLLPAVFFLAAA
                     LFIYIGITRVVTEGGLAATRAPMIPSVITTSTLGYNRIGQTGMAALGLTFAYAADIRT
                     FVMASVANGLKMIEDVRARRRLLFWAILISILVAMVTAIWAVLFFGYRAGAINATRWY
                     FISGPVYPWRYIAEVIKDPGSVHNKLYLNFTLLGAGLAVAFQFLRTRLLWFPFHPLGL
                     AFSTIYLTTQLWFSIFIAWLLKSILLKYAGVRIYERSKLFFIGLIVGQFVVNGIWLII
                     DGFTGKIGNQIFWI"
     gene            complement(12183..13094)
                     /locus_tag="PKN80_00290"
     CDS             complement(12183..13094)
                     /locus_tag="PKN80_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNQ34490.1"
                     /translation="MSRRITAVALGMAREYRQPRTPEDNLKYLEESLAEMAGLKPDLV
                     AVPEVFPYSGLPTLEKKPGLGTETLQKLALRHRCWLVGSIYEKRQGRLYNTALLVNRA
                     GKIAGRYDKIHLTDTELKRGVCPGRPDPEPLKTDFGRIGIQICFDVNWPDETRRLVER
                     GAELIVFSSAFPAGRLLETLGFQNRVPIVSAVQQTPSGILDNAGRWLDRTDRFSWWVT
                     RVLDLESAVFHWDYQGDRLKAIRARYGDRIRIETLGPEAWFILEAADPELSIPAVARE
                     FELRTYRRYIKDSTDHQLKAAKKYYNK"
     gene            complement(13141..13917)
                     /locus_tag="PKN80_00295"
     CDS             complement(13141..13917)
                     /locus_tag="PKN80_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNQ34491.1"
                     /translation="MKIYIMTDIEGVAGVARFFQTREAERPEKKVAMTLLTREVNAAV
                     DGILEADPAAEVVVMDGHGNGGIDITEFHPKARLINRGPLPPPGLLDHGFDGLFFVGQ
                     HARAATENGNLAHTYSSLTIEYYRLNGRLIGEFGCRAALAGSYGVPTVFISGDDRAVA
                     EARELVPDIVGVTVKEGLGLETALHLSPARAREVIRAGAAEAVGRIGAIPPYRVEPPY
                     ELVIRVKEGVSLENFLKKGAEPLDERTVVRRSSSLNDLPI"
     gene            complement(13941..14816)
                     /locus_tag="PKN80_00300"
     CDS             complement(13941..14816)
                     /locus_tag="PKN80_00300"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ34492.1"
                     /translation="MIIDIHVHTVRNHGFPRDGRTALTTPVELLERYRELGVDRAVIL
                     PIIHPECALSLQGVEEVLEICADFPDRFLPFCNIDPRQVGNSAWADLGGLLEFYRAAG
                     CRGVGEVAASLPFNHPMVENLFGHCQRLGMPLTFHIGPALTGCYGLYDLPGLPLLEGA
                     LAGFPELVFLGHSQPFWAEIAPLASPEERNGYPAGPVRRPGRTVELLRRYPNLHGDLS
                     ARSGYNAVSRDREFGLALLEEFQDRLYFGTDICAPDTPAPLAAYLKQLKDERAITPEI
                     YEKITSRNAIRLLKL"
     gene            complement(14813..15868)
                     /locus_tag="PKN80_00305"
     CDS             complement(14813..15868)
                     /locus_tag="PKN80_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34493.1"
                     /translation="MAQRKTGLPTRVLVMGLGRGNAFVNAALNSRELELAGIIDLDAE
                     RLKRVAADWQLRPEQLFTSLAQARSAGPADLLVIATPTHLHADHVVAGFQAGMHVLCE
                     KPLVTTRLQAHRLSTAARRYRRRLAVVQNMRFNPLARRCRELLAEGAIGRLNSLEINF
                     HRWRPALKLGHPHAVLFNHGVHHIDLARSIGGGRPLSVEASEWDPPWWKQSRKGHCLR
                     LTARMSGGWTLCYNASYAEAGRETPHSGEIRLAGSAGSLEMGGEIDRDPQLWLHQVKR
                     AVGQETRRRIAVETGDWRGMDRELLEGFSRAIRKGAPWETDLSDNLKSLEILFRAAEY
                     LEGKRAPAARTRRAGKR"
     gene            complement(16129..16908)
                     /locus_tag="PKN80_00310"
     CDS             complement(16129..16908)
                     /locus_tag="PKN80_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34494.1"
                     /translation="MTGKNNRARLKIAGRSGRKAGPLSAAALAVILLAGSSGRSEPQP
                     AAPAEVIRVLFARRPDPARAPLFPPLRQYTGAPASEKTDIRLATDGKKLRLRIRAYDA
                     HPEKLARQLTDAGIIPAIWKEDSFEIFLKPEADFPENRQFIISNTGRYVLNRNQHLED
                     GRYQRLPWPEPETKEIKIKVKRHRQGYDIELEAPLKSLGFPKPLEAGDRFFAKVIRNY
                     RAHLSPEREVLQIRPARLLLGPEADPANHRIEGYSPLECAR"
     gene            complement(16892..19702)
                     /locus_tag="PKN80_00315"
     CDS             complement(16892..19702)
                     /locus_tag="PKN80_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34495.1"
                     /translation="MRKFVFILALLLGWAVFSPSFSAAAGRNLLWDGGFEIGYGNSYW
                     GVVYGNYGPNFRASWRDGVLKLERPMGSRIYRLEDGDYYLAAWVKRGPGFEKKPGRFE
                     LVLTSRNYDRASQVDNYRKFFEIPEEDRWHQVGFPVKIQNPVNPLMHVELRPEFPDTD
                     ILVEAVSLTKGAEAPAAFEAAAEIEAGFYIPEETCTYVDGEERVVELRIRNNGAARRA
                     RVTWEIYNYREELVRRGEFNEVFPAQTTTRKRLPVADLPHNGYRLACAAEASPLLGDA
                     SVSFLPVINQAAHPKWGACANIGDATRGYTARLMKRLGMSRSYIMSASQPGRWNNVEQ
                     APGKYTWIEEAGLDEAIRQGVEPAVCLEVFKKPPAYVAKYVEKNLLVDEAAYTKAVVN
                     YVDAFVRHYAGRVKAVVLEDETGWPPLPEVARIYAAAYATAKKAARESKCEMEFGING
                     DWKFFEKLIPLVGPDKFDFLSGNYIGQPGGSAAMINAGRKAGWQGRYADIPAVGQRTL
                     PRPTALIIDSPDLGGFTAGEFLWQHVSNLWLNRPYGTEDPKDGPILRTGYYDIRVMSA
                     SPFYVMGRKSGAEYDNSPALGFQAVAMLKHLTEGMRPARPAGGELSITGLPTANPGVW
                     LYPFRNEKKALVALLAGNQAMNNKAWIISGLDFSQHRPLDFYGNPRPFENNRLRTPEL
                     PVYFETAPEKLDGLLAALAKAEIALDPAGDHQVVRSGPYILEFGPDLANLFQLRLARD
                     GRELVLLDGLECEPKAGFGEVTFIPGRLVNSIDISLTAASDLYRRRFLTFRLTPEGVQ
                     VNWNQSNAHPALVKTSVRFRLGKELTGRELRVDTDGQARTGRLGADGSFTESAAPAAS
                     PLKAAAGRLVVKGLAVIDLPAATGQGVFRPATGLRFRAEAGREAALAADYEINPIPVP
                     GSTAAIQKIYLKMLITADDGKK"
     gene            complement(19891..21045)
                     /locus_tag="PKN80_00320"
     CDS             complement(19891..21045)
                     /locus_tag="PKN80_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNQ34496.1"
                     /translation="MKRTNIPAYLRIEHDLRKKLESGRLKPGDRMAPEYELAEEYKVS
                     RMTLRKGLSLLVSEGYLYQVPGRGTFVTSPDDQSLEVMRKQRDKLRKMNRGVGMLIPS
                     VTVSLYLGIVRGAEDACRENGFHLVLGNYDGVPEKEKEYLSMFADRGISGLIVAPSYN
                     SNLNNGYRNLVEKKIPVVLTGLELSGLTADLVATDYFNSAYLGIEHLVRSGSRKIVSY
                     ASQYVTYASREKVAGFREALRKNNLEAPETFFREVPLKKPLDPDETIGFFQQEEVDGI
                     FSGNESTTIKLINFLNLFAGRLPRKIRLVCFDRPMIVPQSNTPVAYIIQPTHEIGRTA
                     VNLLMARIKEKRTHQPQPYKKVLLQPKIQMSDEEPAWNLEPDSPLESKPF"
     gene            complement(21274..23208)
                     /locus_tag="PKN80_00325"
     CDS             complement(21274..23208)
                     /locus_tag="PKN80_00325"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNQ34497.1"
                     /translation="MNTRMTAAEKKELLGRLDLKSPSLAPLDLAAWPLNERWTGRRLH
                     QALVERVSPRPYLTADDYREEARRLNSEAPAAAAAYRKLGRELLTLARAGEARFWNDT
                     RFDHLHGLGWAAALTGDPAYLRQGEAVLAHHCCQLPPPPGGKHRGGFGWDGLTVGRYL
                     SYATEFLYLAAAAGLIEDEVLARVLLAGHRKLAFTHRHRRDFHFSNITLVGAIGSFFW
                     ATLFPEFKDADRYREEWGRFLADNVEIIFRSDGGNYEQSPNYHSVCQYWYAYAWRQDR
                     VNHKMLPDRVIRRLEKSAEFFSALRMPGGELPPVGDTGLRRRDLSLALTALTCGRPDL
                     LHGIGKLPELGWHLGGRFVTADFSSIKPPKKLNYAFPKSGFYPMRSGWKKEAAYALFL
                     CGPHGMFHGHFNLLSLIVSAGQNLLITDPGAYSYDNSPNRLGFLSTPFHSTLSLHNIS
                     HRPWEDGQPPLARVTQWEEAGAGLLATAVSRAYDHLHGRPVLTRQFYYDGDRTWIICD
                     RLESDSWYAFSQNFILPQGLARKRVQAGSNLWLGVTGGRGPRLMIAQADAHLCAHIQA
                     TGLPVRRQAKTAPQKLCFIKNETRTGYVTLLRILEKGEKIGQISLHMHKEAGHRMRVE
                     AGYANRRVELLFGGRVTLKK"
     gene            complement(23256..>23770)
                     /locus_tag="PKN80_00330"
     CDS             complement(23256..>23770)
                     /locus_tag="PKN80_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34498.1"
                     /translation="PDRYPMPREVHGGFGAWSFEWYVVYLHNNFFDFPPSDHIHPLLM
                     VRDRFNRNRNWRRFDPKTGAGRGNVYVCKGMVIEEADPYHLNYPINQAEIRLLRNGKS
                     LKVRFHHNLPNFSHLEAAADGRKYRFENQREAELEWTPRRGEGRLSVRGVNTRGVAGP
                     ESRVVISSAA"
BASE COUNT         3897 a         7398 c         8328 g         4147 t
ORIGIN      
        1 tgccctggta cccggaggtg gtcgaccagg tgaccgaggc ggtcaagtcg atggcctggg
       61 ccggcggcga aggtttctcc ctttacgacg gccgcttccc cggcggcgat ttcacccagc
      121 tggacgccta caagtgcctc ttcgaatacg acggttccct caagccttcc ggggtggtgc
      181 tggcggtgat ggccaacctg ctggacgggt tccggggcgt gggccagccg gtcatcaacc
      241 ccgtcctgga taccttcgaa ctggaggcgc cggcccgttt cgccttcgcg gtctgcgggg
      301 ccgaccgccg ggtgctggag ggcagcgtgc ggctgccgga gggagtccgg gcctatgatt
      361 tcatgggcaa ccagctggac ccggccgccc cgctgctctt ccccaactac ggcctgcttt
      421 acctggtcgg cccccgggaa cggctggagg ccacccgggc ggccctggcg gcggccgagc
      481 tcaatccgcc ggtggaattg aaactggagc aggtcctgga cgagaagagc ggccagtacc
      541 gggtaagggc cacggtgacc aaccggcggc cggaccggcc gctgaccggc aaaatcctct
      601 ttgacgcgcc ccagctgcgc gatttccggg agaaggtcca gccggtcgtc ctgaagccgg
      661 gcgagagccg ggatatctac ttcggcctga actattaccg gggtgaccgc ttcgagccgc
      721 ccgccacccg tctcaatctt tatttcgacg gcgtgcggtc cgaccagacg ctctccggtt
      781 tcaccgagac ccattcgctg cgcctgcgga aggccgacgc gcttctgaag gagaaggatt
      841 ttgccggggc cgaggcgatc taccgggagc tgctgccggt gatgtcggtc gggcaggcct
      901 cggagaccgg cctcaagatc gcccaggccc tgcaggggca ggagaagtcc gaggcggcgc
      961 tggccgagtt ccgccgggtg gccgaggggg ccggcggccg cccccaggac cgggccgccg
     1021 cctggctggc cctggccgaa aatcacgagg ggcgccagga gattgacgcg gccgcggccg
     1081 cctaccggaa ggcgggcgaa ctggagggcg ggccggccgg aacggccgcc accgcctggt
     1141 accagatggc ccggatgctg cgcgcccgca accaggcctc cagccgggat gccgaaacgg
     1201 ccctggaggc ctaccggaag tcggcggcca tcccggaggc ctgggcaaac atcggcggct
     1261 gcgcccaact ctacctggcc cacttcctgc gcgacctgaa gcgttacccg gaggcgcggg
     1321 ttgagtaccg gaaactgctg gataagccgg gcgcgctggc catgtatgtc aagcaggccc
     1381 gggaggccct ggaggcgctg gagaagtcgc tggccgcgcc ggcggctcgg tgaggaaagg
     1441 gtttctcttt aaggaagggt ttgcttaaaa aaatacgggg gcgggccgtt ttcagacggc
     1501 ccgccccatc tttttttagg ttatttgaat tccccgatca ggcgcgcgat gtgtttcatg
     1561 atcaggtcgg ggtcttcatt ctcgaagccg tagtggtgga agatgaccgc gtcgggccgg
     1621 gtctgcagga ccttccgggc gttccggtag taattctcct cctcgccggc cggcacgtcg
     1681 aagttctcgg gcagtaccag tttcttcttt ccgaaacgtt cggccaccgg atagatgacc
     1741 tcgattgatt ccagcagcgg tttcttccct atgtcggcgc cgttttcctt gacctggcgg
     1801 caggtcggcc cgtcgacgcc caggtaatcc aggtccgctt cccgggcggt cgcctcgtag
     1861 aacgacggtt cctgccaggg catgtggaag aggtaggtgg ctccgcccgg gacggtccgt
     1921 ttgaagagag tgcaaacctc gcccagaaac ttggccaggg tcggaatgtg gatttcggcc
     1981 ggatcgccca atttccggca gtgcgggcag taacagggcc agttggaatc cttcggttcg
     2041 tccatgatca gtccgtcggg ccggaacttg gcgaaggcgc gctcggccac ctcgaagaag
     2101 tactcccgga agccggggtt gttgacgcag cagaccaggg cgccgggcgg gttgccctcc
     2161 gggtcccgct tccaccagtc ccggtgctgg aaggtgcggc gggaggaagt gttgacggcg
     2221 gcggccgcga agaggccgcc ccaacggcca aaggtgaaat aggggcgcag gccggtctcc
     2281 cgcatcagct cgatatgctt gagaatggat cgcggcacgg caccgacgtc ttcgatggtc
     2341 gcggcgaagc cgaccgcgtc tccgcccagg tccttgatct cgcggtaatc gtcgcggatg
     2401 gcgtccggct ttaaaacata aaaggcgtgg ttgtaacgat agaagatgaa ctccatattt
     2461 tcggcccccg cgtggtttgg gttgatcgcc ggattgccgg ttattggaag tcccgctcgg
     2521 gatcgatgac caggaaggcc atctgatagg cctggccgcc cttggtgaag gagcgcttga
     2581 tcaccggatc gccggccgcg ttgtagaggg cggccaccgc gtaaattttt ccatccggac
     2641 cggccgagag cgcgtggatc tcggcggccc gccggccttc ggcgtaaatg gtgccgtggt
     2701 cggtgaaggt ccctttgtcc aggtcgtagg agaccaggtt ggtaaagcgt ccctcgcccc
     2761 aggccgtgta gtatattctg cggccggaac ggcagaagcc gagggcggcc ggccggggat
     2821 aattcttccg gagtccaaag tcgccgagag cttcgaagcg gccgctttcg ggatggaagc
     2881 ggcagaggag ttcggtcccg tggaccagca ggtagaagca cttttcggcc gccgcccaga
     2941 agccggagcg gatcacgaaa gaacggccgt tcttatcgtc cggcttgaag gcgggcgcgt
     3001 agggggcctc cggcagccgg aggtccagct cctcgatccg tccggacgcc gggtcgaaac
     3061 gccagagccg gccgccgctc cgggcgctgt aaaccacgcc ccggccgtcc accagcagca
     3121 tccggctgac cggtcccggc ggttcggcga tgcccaggtc ccgggtccgg ccgctcttca
     3181 ggtccaggcc gaccaggtgg ttcggtccct ggccgtgatc acccggccag gagagagcgt
     3241 agaagacctc ccggcccggg tcgagggcgg cggtctcaag tccctcgccc ggccgcggaa
     3301 tcccgaaatc ggtgaaacgg ccgctcttca ggtcgtagga gatgatgtgg ccgcccgggt
     3361 aatcggcttc acccgcggcg gtgtagtcgg catgcagggt ggtgaagtaa aggcgtccct
     3421 tgtgttcgga gatctggctg tggatcttac cctgggcggt gcgccccgaa ttcgtttcgc
     3481 cgcagacctc gcccaggtcg gccaggtggg tcaccttctt cagcttcgga tcgaagacga
     3541 agaactgggt gtggtgtccc ttttcgtggc tggccaccgc gaagtaggtg cggccgtcgc
     3601 tggcggtgat gatgccgtgc cactggccgt ccaggtacgg gtattcggct gagaaggagt
     3661 ggaaccagcc ggcgttgacc cggccgggct ggcgggccgc ggcggccgga ccggccggca
     3721 aaacggccag gtcgggaccg gccggcaaat cggccaggtc tatcttgaga agccgcaact
     3781 ggtagggaac ccgggccgga ccgtcggctc cggccacggg cagggtggtg aagggcacgc
     3841 tccggtcgcc ggtcttcgcc tcaaccgcgc ccagcaggta gagcgtccgg ccgtccgggc
     3901 tgaccgcggc gctctgggag ccgaagaggc gccggccgtc ttcgcagaga atcgggccga
     3961 ggtcctgcgg ctggccgtcg ccgggccgcc ggaccaccag gtgggccggc ccgttcaggg
     4021 gctgggagtt gaagtagtcg aagaggccgc gggccgggac atagtaaagg gtggcgtcgg
     4081 gggcctgggc cagggcgagg gtggcgtagg tgagcttctc ccggccgatc tcctcccggc
     4141 tgcagacctg gccgaggtcc ttgacctggc cgctggccgg gtggaaggag aatagtttcg
     4201 aggttccgcc gtgcaccccg tagaagagtc cggccgtccg gtcccagacc acggtccgcc
     4261 agatccggcg cccgacggtg tttccggccc attccggggc ttccaggtcg agggagggaa
     4321 cccgggtcgg caggacctgg agccgccggg cgcggcggtc gtacctgacg atcaggtccg
     4381 gttcccggga aaagtaggcg ttgccgtccg agtcgcagcc gaggctccgg caaacggtct
     4441 tgccgtcggt cgccgggccg aggttgctga cccggccccg gcgcaggtcg cagaccagaa
     4501 agagtccgga aggataggta aggccgtaca tgagctcctg ctccggggcc agctcggcgc
     4561 tgatgatgcc ctcgccggcg ggcgcccggc ccaggacccg gcaccggccg gtcttcggat
     4621 cgtaggaaag gaaggcgccg cccgggtaat cgatcttcgc cttcaggtcg ttgagcgggt
     4681 agctgaagtg ggtggcgaag tagaagcggc catcccgccc ctcccggatc cgggtgtgga
     4741 gtttcccctg gggaaggcgg ccccgcccct tctcgccggt ggcccggccc aggtcggccg
     4801 ccagccgggt tcggccgccg gtcgggtcgt attccaggag taccgcgtcg tcccggccgt
     4861 tatgggtgca gaggccgaga tagacccggc cgttggcggc ggccagcagc ccgtgatagg
     4921 cgccgtccat ctcctcgtag cccgggaagg cgacggactc ggccctgacc tgggccccga
     4981 gccggtccgg ccgcagcagg aggccgccca gcagcaagac ggtaagcagc aggaaagata
     5041 ttctttggcg ccgattcatt ttttgcctcc cgtgtgattt tcattgagcc gcccggcggg
     5101 cggtttttcg tgcttttttt cagcggcgca gcagccagaa gacgaagcca atgttcagaa
     5161 tcgccaccag caggccggcc aggaagccca ggaccgaggt ccgggagggg accaggattt
     5221 ccagttcggt gccggggaag aggcggcggc gcttgaccgg caccgttccg gccggcaggg
     5281 tgcagggttt ctccgtctgt tcgcccggct ggaccggggt ggcgaccagg gcgtagaaat
     5341 tgtcgaggcg ctcctttggg atgttgcggg tgaagaggct gaccaggagg ccggccagca
     5401 cgccgccggc cagcgaaaag accatctgcc agggcaggta gatctcgggc cggccgccct
     5461 tgaggatcac cagtttcagc ccctccaggc cggagaggcc ggccagaaag gcgacgaacc
     5521 aggcccgggt ggtcagccac cagacggcga agccggccag ggtcgaggcc caggcgccgg
     5581 ccggggtcat gccgcgccag aagaggccca gccagaaggc gatgcccatc atgggcggga
     5641 tcatccagaa gatttccagt cccttgagga cgcctggaaa gaggtaggcg aagatgaggc
     5701 cgccgaccac gaccaggagg gtcgcccagc gtccgatcgc caggtagtgt ttctgatcgc
     5761 ggttcggccg cagcggccgg taaatgttct cggtgaagag ggcggccgag gcgatcatga
     5821 aggagtcgca ggagctcatt accgaggcca gcagggcggc cagaaagagg ccgagcatgc
     5881 cgtgggagac gcggggcagg aactcccggg ccaccgcgcc gaagacctgt tccgggtcca
     5941 tcgcctggcc ggcgaagtag gcgaccgccg ccaggccggt cagggtccag ccgaccgtgc
     6001 agatgcgctt gatgaagctg ccgaacatga agccgacccg gccgtccatc tcggtgcggc
     6061 cggcggcgca gttgcccatc gtgtgcggct gggccacgat gccgaccagt ccgttgaggc
     6121 tgatgatgat gatgtagaag aggccgattt cggccggcgc caccagggag aacatggccg
     6181 gatcggtgat cgaggctttc agaccggcta tcccgccggt cgcgtgcagg accaggggca
     6241 tcagcatgaa ggagaagacg atggtcagga tcccctggaa gaagtcggtg atgatggcgg
     6301 cggccagccc gccggccatg ccgtagatga cgacgatgac cgtcatcgcc cagattgccc
     6361 agtcggccga gaggaggttg ccggtgcagg cgacgatgac cttgcccgag cccttgagca
     6421 ttacgcccat ggccaccatc agctggagga tgcccaccac ggagaagagc atggccacgc
     6481 tgcggttgaa gcggtactca aagacgtcgg cggtggtgac ggcccggaag cggcgcatca
     6541 ccggggcgat gagccagtag aagggggaga cgaagagcca gagccactgg taccagatgc
     6601 cggagacccc gttggtgaag gtcttggccg ccacgttcac cgcctggtcg gaatgggtgc
     6661 cggtgccgaa ggtgtgcatg agcatcatcg ccttgccgaa gcggcgcggc atgaagaaat
     6721 cggcaatccc ggagaggccc cggccggcca tgacgccgaa gtaggtggtg gcggccaggt
     6781 agatgatcat gaccagccag tcggcctgag aaagtcccag catgttccct cgctcgggtt
     6841 agcgtgaaag gtacggcctg tttaccagat gctgatggcc cggacgccgc ccggaccctc
     6901 caggcgccag gcggttccgt ccgtctcctg cagttcggcc cggaggccga gggcctgccc
     6961 ggtcttcagc ccgagcgtct tcagcggcag ggccaggtgg atgaagagat cctccggccg
     7021 ttcggagcgg acgcccttga gcggcggcgg agcctggccg gcggccaggc cggtcaactc
     7081 ggcggaaccg tccccggcct gcaggcggag caacggttga agtgtttcgc cccagggctg
     7141 gcgggcgagg tccgggatga accggacctc caggctggcc cggggatcgg ccgaactctt
     7201 cagcaggtgc aggtccaggt aaaggttctc accgtcgcgg ctgaccgcca gtttccgtcc
     7261 ctcgcccagg gtcagcatcg ggtagccggc cccgtggatc gagggaaagt tcttgaagcc
     7321 gccttcggcg acccggttgg cccggtagcc ggccaggtcg atcggaaggc gggtcgaaaa
     7381 gaggcggctc accagttcca ggttcatcgc ggcgccgggc ggcaggggta tttccgggtg
     7441 gctcaggcag gggaaggcga ttcggccggt ttcgcccggc ttcagggtga ggttgaccgg
     7501 aagatcgaaa cggcggcggt tgccggcgac cgcgaaccag ctggcggttc ccagctgggg
     7561 cagggccgtc agttccaggg gcgtggagag gttgtttttg agccggatca cgaaatcggc
     7621 ggcgcgggag ccgggggtct cctccacgta ggtgacgaag tccagggccg gctccagccg
     7681 cagaccggcc aggcgctccg ccaggtccgg cccgccggcc aggtagcgcg ggttgctgtc
     7741 gaggatgatc tccccgttgg cggagagcgg ctggaccgcg ccggtcgtgt cgcgccgggt
     7801 gagcccgtcc gttttcgtca gccggccctt caggatccgg ccgtcggtcc agagcgccgc
     7861 cagccgccct ccgtcccttt cgaactgata aacctgaagt ccttgaaacg gggtggcccc
     7921 ggccagcggc cggctgccgg ccagcaggtt cccggccgcc gcgtaggcga tgccggccgg
     7981 ccggaaggcg ccgtcgaact caaagaggga ggagcgcgac cagtaggtac ccggttccat
     8041 gcccatgatg cgggcgtcgt aatggaagaa cctggagaca tccagttcgt ggagcctttc
     8101 cacgaagaac ttggccagga cgccggcggt ggcgccggtc ggctgctcgg tgtagaggct
     8161 gtccttgctg ccgccgaagg tgatggccca gacttccttg ccggcggcgc gggccgcctc
     8221 gatctggggc ttggtgttcg cggccaggtt ggccccgccg gccagaatat cgtaaaaatc
     8281 gtgcttgccg ccggcggccg ccatgacctg gccgaagaac ttgaccgagt ccccgtgcat
     8341 gaccaccgag gcggccgggt cgattttttg gaccgcctgg cgggcggcgg ccaccatctt
     8401 ctggtaatga tcggccggca cccggccgcc gggttcgtcc tccaccatcc agtaatggac
     8461 ccggccccgg tagtgttcga ccattctggc cacgaattcc gtccaggccg ctatggaagg
     8521 ataagtcttg cccggctcca gggcccaggc cgggtagcgg ttgccgaggc agaccaccac
     8581 ctcgaccccg ttctggttgg ccaggtcgat ggcccagtcg taccagaggt aacggtcccg
     8641 ctccggggcg gccagggaca tccggccgat gccggccatg gcgctcaggg tgttcaggta
     8701 gcggaagccg agccggtgga agatgggcac cgattccggc gaaagggtgg cgtagagccc
     8761 ggtccgcagg tcggcccggg gccggtcggg cggcggctcg atcagggcga aggccttgcg
     8821 gacggccggc gccgcgccgg gggccagcga gcggaccacg gccaggtaag cgccgggctg
     8881 gctgagttcg aggcgtacca gctggccgcc ccgccccttt tcgacggcga tgttcagccg
     8941 cttttccagg accggctgca ggtttaagtc caggatcttg agctcgattt cggccggagt
     9001 gccggcggcc gccacctcga cccccactgg ctcgccggtg tagaagaccg cgcccggctt
     9061 gccggttatg accagaccgg cctccgattc gtaggcgctc cggtaggccg tcaattcacc
     9121 ccgttccagc tggatggcgt cggtccagat atcggtcggc gcggccgccg ggtcgggctg
     9181 cttgtcgttc cagagcacgc tcacctggat ggttccggta aaatcggtcg gcagttcgaa
     9241 gctggtcgag gtccgcttcc attccggccc ggtctcgagc ggggcggtga agaccgccgg
     9301 ccggctctcc aggtcccgga gggcggtggc ggtcaactgg accgcggccg gcttgagcgt
     9361 cctgaagtag ccgctcaggg tgaagcggcc gccccgggtg accggcaccg gccggttgac
     9421 caggcggacc ggtccgatgt cgggcaggcg caggcagaag cggccgtgcg gggcatcaac
     9481 ggcggcttcc aggtaatcgt caatgatgaa gccgcggctg gcataggcct tgccccagag
     9541 atcgttgagg ccggcctcga agctggaacc cggcagcagg ttgccggccg gctcgccgcc
     9601 ggagcccgga agcggaccca gtgccacccc gcgccggaat tcgccctggt cgcccaggtt
     9661 gctggaaagc cagacccagt agaggcgaag ttcgcgctgg ccgatctccc gggcggtgat
     9721 ggtcagcaga tcgcccggcc gggtggtggt gagaacggcc gtcatcgcct cccggttgaa
     9781 gcgcgcgccg tccatcttca ggcgggcgac ggcgccgttc aggtcaactt caaactggtt
     9841 gaggccggtg tccacgttcc agctctggac caggacccgg taggagccgg gcggcagctc
     9901 ccggtcgagt cgggcctgga tggagctgcc ggtctggcgg gtgccggcgt aggagcgccc
     9961 ggagccgagc agggcccggc cgaacccgtc gagccggtcg aagtaggcgg cgtcccggcc
    10021 ccagtgatac tcctcggcgg ccggccgggt ctgggcccgg ccggcggcgg ccgccgtcag
    10081 ggttaaggcg gccagcagcc cggccgcgat catccgatag cgcatggtgc ctcccggagt
    10141 atttgcgtgt ttggcccgcg ccggatcaaa atccggcgcg ggcgatgggt tgagccgccc
    10201 ggtccggttc agatccagaa gatctggttt ccgatcttgc cggtgaagcc gtcgatgatc
    10261 agccagatgc cgttgacgac gaactgtccg acgatcaggc cgatgaagaa gagcttggac
    10321 cgttcgtaga tcctgacccc ggcgtacttg agcaggatgc tcttcaggag ccaggcgatg
    10381 aagatggaga accagagctg ggtggtcagg tagatggtcg agaaggccag cccgagcggg
    10441 tggaagggaa accagagcag ccgggtgcgc aggaactgga aggcgaccgc caggccggcc
    10501 ccgagcaggg tgaagttcag gtagagcttg ttgtggaccg agcccgggtc cttgatgacc
    10561 tcagcgatat aacgccaggg atagaccggt ccggagatga agtaccagcg ggtagcgttg
    10621 atggccccgg cccggtaacc gaagaagagg accgcccaga tggccgtgac catggccacc
    10681 aggatggaga tcaggatggc ccagaagagg aggcggcgcc gggcccggac gtcctcgatc
    10741 atcttcaggc cgttggccac cgaggccatg acgaaggtcc ggatgtcggc cgcataggcg
    10801 aaggtgaggc ccagggcggc catgccggtc tgtccgatcc ggttgtaacc gagagtcgag
    10861 gtggtgatta ccgaagggat catgggcgcc cgggtggcgg ccaggccgcc ctcggtcacg
    10921 acccgggtga tgccgatata gatgaagagg gcggccgcca ggaagaagac cgccggcaac
    10981 agcggcagtc cgctggccac cagccagccg accagaaaga ccaggcagat tatcgtgccc
    11041 cagaaggcca cccggtaggg aacgatttca tcggcgtcgt ccgggttcgc cttcggccgg
    11101 accgcggcgg tgaagaccga ggccagttgg cgccgggcca gccagaaact gtaaagcatc
    11161 agggcgacca gggcgcccag cgactgccag tcaaggatcg gccgggcggt gtaaggaagg
    11221 tagccggtgg ttccgaaacc ggtgatgttc aggaagccct gctcgaccgt ggtgagcagg
    11281 gcgaagaacc agaggctgaa ggacatcggg atgttgacga agtaggtaaa gccgatcatc
    11341 gggaaactga cccggaaggg aagctggatg gtcgcccgga agatcgggat ggtgttgtca
    11401 agtttgaggg ccggcaccga ctcgaagtag aagtgcagcc cgttgaggga gccgatcagg
    11461 gcggtgatgg cgaatccggt ccagaagaga gggttgcgaa agaggttgtt ggcgtgggca
    11521 ttgatcatct ccagcggggt cttggtaagc ggataattca atatttcgtt ctgttcccac
    11581 tgccggcgca ggatgaccat caggaagatc atcaccaggt agagagccat gagaaaggcg
    11641 gcccagggga gcaggggctt cacccaggcc agccagggta tggactctcc cttgggcagc
    11701 ccttcgtaga accaggcgac cgcgttttca tcccggacga tcagccaggg tttgaggtag
    11761 gggatgatgg tctcggccca gcggttgctg ctggtggcgt agtagctggc ggaactgatg
    11821 gtcgggatca ggtaaaggat caggcccatg gtcgggatgg cgcaggccac gatcatcatc
    11881 acgtagacca ggatcatctc ggcgctcgag aaggccaggc ggcggcagta ccggcgcagc
    11941 aggaggttga gcaggaccag ccagaagagg aagaagatgg ccgaggaggc ggtgaaatct
    12001 tcggccatgt aggagccctg gacggcgatc tggttataga cgttgcccag ggcgatcagg
    12061 gcggccatga ccgcgccggt gatgatcgag cgcagggtga attcgttctt ctccaatttt
    12121 tccatgcttc tctccgcttg ggtgacccgc cggacccggc agaccgggac aaccggtttt
    12181 ttctatttat tataatattt tttggccgcc ttgagctggt ggtcggttga atccttgata
    12241 taccggcggt aggtccgcag ttcgaactcc ctggccacgg ccgggatcga gagttccgga
    12301 tcggccgctt ccaggatgaa ccaggcctcc gggccgaggg tctcgatccg gatccggtcc
    12361 ccgtaacggg cccggatcgc cttcagccgg tcgccctggt aatcccagtg gaagacggcg
    12421 ctttccaggt cgagaacccg ggtgacccac caggagaagc ggtcggtccg gtcgagccag
    12481 cggccggcgt tgtcgaggat gccggagggg gtctgctgga ccgctgagac gatcgggacc
    12541 cggttctgga agccgagcgt ctccagcagc cgtccggccg ggaaggccga ggaaaaaacg
    12601 atcagttccg cgccccgttc caccagccgc cgcgtctcgt cgggccagtt gacatcgaag
    12661 cagatctgga tcccgatccg gccgaaatcg gtcttgagcg gttccgggtc cggacgcccc
    12721 gggcagaccc cccgcttcaa ttcggtgtcg gtcaggtgga tcttgtcgta acggccggcg
    12781 atcttacccg cccggttgac caggagggcg gtgttataaa ggcgcccctg ccgtttttca
    12841 tagatggagc cgaccagcca gcaccggtgg cgcagggcca gtttctgcag ggtctcggtg
    12901 cccaggcccg gtttcttctc cagggtgggc agtccggaat aggggaagac ctccggaacg
    12961 gcgaccaggt ccggcttcaa gccggccatt tcggccaggg actcctccag gtacttcagg
    13021 ttatcttcgg gcgtccgggg ctggcgatac tcccgggcca tcccaagcgc caccgcggtt
    13081 attctcctgg acatcgcgct cctctctccg gggaccgggt ttcgttccgg cctggatgct
    13141 tcaaataggc aaatcgttga gtgaagaact tcttcgcacg accgtgcgtt cgtccagcgg
    13201 ctcggccccc ttcttcagga aattttcgag ggagacccct tccttgaccc ggatgaccag
    13261 ttcgtagggc ggctcgaccc ggtagggcgg gatggcgccg atccggccga ccgcctcggc
    13321 cgcgccggcc cggatgactt cccgggcgcg ggccggcgac aggtgcaggg ccgtctccag
    13381 gcccagcccc tccttgaccg tcaccccgac gatgtccggg acaagttccc gcgcctcggc
    13441 cacggcccgg tcgtccccgg agatgaagac ggtcggcacg ccgtaactgc cggccagggc
    13501 ggcccggcat ccgaactcgc cgatcagccg gccgttcaac cggtagtatt cgatggtcag
    13561 ggaggaatag gtatgggcca ggttgccgtt ctcggtggcg gcccgggcgt gctgtccgac
    13621 gaagaagagc ccgtcgaaac catggtcaag cagcccgggc gggggcagcg gcccgcggtt
    13681 gatgagccgg gctttcgggt ggaactcggt gatgtcgatg ccgccgttgc cgtggccgtc
    13741 catgacgacc acctcggcgg ccgggtcggc ctccaggatg ccgtccaccg cggcattcac
    13801 ctcgcgggtc agcagggtca tggccacctt cttctccggc cgctccgcct cccgggtctg
    13861 gaagaagcgg gccaccccgg ccacgccctc gatatcggtc atgatgtata ttttcattat
    13921 ttccttcggg aaccgccggc tcagagttta agcagccgga tcgcgttccg gctggtgatc
    13981 ttctcgtaaa tctccggagt gatcgcccgc tcgtccttga gttgtttaag ataagcggcc
    14041 agcggcgccg gcgtatccgg ggcgcagatg tcggtgccga agtagaggcg gtcctggaac
    14101 tcctccagga gggcgagtcc gaactcccgg tcgcggctga cggcattgta gccgctccgg
    14161 gccgagaggt cgccgtgcag gttcgggtag cgccgcagca gctcgaccgt ccgccccggc
    14221 cgccggaccg ggccggcggg ataaccgttg cgttcttccg ggctcgccag gggcgcgatc
    14281 tcggcccaga agggctggga gtggcccagg aagaccagtt ccgggaatcc ggccagggcc
    14341 ccttcgagca gcggcagtcc gggcaggtcg tagaggccgt aacagccggt cagggccggc
    14401 ccgatgtgga aggtcagcgg cattccgagc cgctggcagt ggccgaagag gttctcgacc
    14461 atcgggtggt tgaagggcag gctggcggcg acttcgccca cgccccggca gccggccgcg
    14521 cggtagaatt ccagcagtcc gcccaggtcg gcccaggcgg agttcccgac ctggcgcgga
    14581 tcaatgttgc agaagggcag gaaacggtcc gggaagtcgg cgcagatctc cagcacctcc
    14641 tccacgccct ggagcgagag agcgcactcc gggtggatga tcggcaggat gaccgcccgg
    14701 tccaccccga gttcccggta gcgttccagc agttcgaccg gggtggtgag ggcggtccgg
    14761 ccgtcgcggg gaaagccgtg attgcggacc gtgtggacgt ggatgtcgat gatcatcttt
    14821 ttccggcccg gcgggtccgg gcggccggcg cccttttgcc ctccaggtat tcggcggccc
    14881 ggaagaggat ctccaggctc ttcaggttgt cggagaggtc ggtctcccag ggggcacctt
    14941 tccggatggc ccggctgaat ccttccagca gttcccggtc catgccccgc cagtcgccgg
    15001 tctcgaccgc gatccggcgg cgcgtctcct gtccgaccgc ccgtttcacc tggtgcagcc
    15061 agagctgggg gtcgcggtcg atctcgccgc ccatttccag gctgccggca ctgccggcca
    15121 gccgtatctc cccggagtgg ggggtctcgc ggccggcctc ggcgtaactg gcattgtagc
    15181 agagggtcca gccgcccgac atgcgggcgg tcaggcgcag gcagtgtccc ttccggcttt
    15241 gcttccacca gggcgggtcc cattcggagg cctcgaccga gaggggccgg ccgcctccga
    15301 tcgaacgggc caggtcaatg tggtggacgc cgtggttgaa gaggaccgcg tgcggatggc
    15361 ccagtttcag ggccggccgc cagcggtgga aattaatctc caggctgttc aggcggccga
    15421 tggcgccctc ggccagcagc tcccggcacc ggcgggccag ggggttgaac ctcatgttct
    15481 ggaccaccgc cagccggcgc cggtaacgcc gggccgccgt cgacagccgg tgcgcctgga
    15541 gccgggtggt gaccagcggc ttctcgcaga gcacgtgcat gccggcctgg aagccggcca
    15601 ccacgtgatc ggcatgaagg tgggtcggcg tggcgatgac cagcaggtcg gccgggccgg
    15661 ccgacctggc ctgggccagc gaggtgaaga gctgttccgg ccgcagttgc cagtcagcgg
    15721 ccacccgctt caatctttcc gcatccaggt cgatgatgcc ggccagctcc aattcccggg
    15781 agttcagggc ggcattaacg aaggcgttgc cccgtcccag ccccatcacc agcacccggg
    15841 tcgggaggcc ggtttttctc tgggccatgt tcggtttccg attggcagcg ttaaatttcc
    15901 gtctaaaaga gaccggcgcc gggccttctt tcaggcccga cgcctcgtct cgaatttctt
    15961 gaattaaccc gccccgatta taccgggcgg cggccgggca ttaaactcct attttacctt
    16021 atcggcgggg ccggcggcca gcttcaggat cggccggctc cgccgcggcc gcttccttga
    16081 caatccggtt ttgagggcgc ggaaggccgc ctccggccgc ggctcccgtc accttgcgca
    16141 ttccagcggg ctgtatccct ctatccggtg gttggcggga tccgcctccg ggccgagcag
    16201 gaggcgggcc ggccggatct gcagcacctc ccgctcgggg ctcaggtggg cccggtagtt
    16261 tcggatcacc ttggcgaaga agcggtcccc ggcttcgagg ggcttgggaa acccgagcga
    16321 cttcagcggc gcctcgagct cgatgtcgta accctgccgg tggcgcttaa ccttgatttt
    16381 tatctctttc gtttccggtt ccggccaggg caggcgctgg tagcggccgt cctccaggtg
    16441 ctggttgcgg ttgaggacat accggccggt gttggagatg atgaactggc ggttctccgg
    16501 gaagtccgcc tctggtttga gaaatatctc gaagctgtct tccttccaga tggccgggat
    16561 gatgccggca tcggtaagct ggcgggccag tttttccggg tgggcatcgt aagcccggat
    16621 ccggagccgg agtttctttc cgtcggtggc caggcggatg tccgtctttt cggaggccgg
    16681 cgcgccggtg tactggcgca gcggcgggaa gaggggcgcc ctggccgggt ccggccggcg
    16741 ggcgaagagc accctgatca cttccgccgg cgccgccggt tgcggttcgg accggccgct
    16801 gcttcccgcg agcaggatga ccgccagggc ggccgccgat aacggtccgg cctttctccc
    16861 ggaccggcct gcgattttta agcgggcccg gttatttttt cccgtcatcg gcggtgatga
    16921 gcatcttcag gtaaatcttc tggatggcgg ccgtggagcc gggcaccggg atcgggttga
    16981 tctcgtagtc ggcggccagc gccgcctccc ggccggcctc ggcccggaag cgcagcccgg
    17041 tcgccggccg gaagaccccc tggccggtgg ccgccggcag gtcgattaca gccagaccct
    17101 tgacgaccag gcggccggcg gcggccttga gcggtgaggc ggccggtgcc gccgactcgg
    17161 tgaaactccc gtcggctccc agccggccgg tccgggcctg gccgtcggta tcgacccgca
    17221 gctcccggcc ggtcagctcc ttgcccagcc ggaaacggac cgaggtcttg accagcgccg
    17281 ggtgggcgtt ggactggttc cagttgacct ggactccctc cggggtcagc cggaaggtta
    17341 aaaagcgtcg ccgatacagg tcgctggcgg cggtgaggct gatatcaatc gaatttacca
    17401 ggcggcccgg gataaaggtc acctcgccga agccggcctt cggttcacac tccaggccgt
    17461 ccagcaggac cagttcccgg ccgtccctgg ccagccgcag ctggaagagg ttggccaggt
    17521 ccggtccgaa ctccaggatg taggggccgg agcggaccac ttggtggtcc ccggccgggt
    17581 caagggcgat ttcggccttg gccagggcgg ccagcagccc gtccagtttc tccggggccg
    17641 tctcgaagta gaccggcagc tccggggtac gcagccggtt gttttcgaag gggcgcgggt
    17701 tgccgtagaa gtccagtggc cggtgctggc tgaagtcgag gccgctgatg atccaggcct
    17761 tgttattcat ggcctggttt ccggccagga gggccaccag ggccttcttt tcattgcgga
    17821 acgggtagag ccagacgccg ggattggcgg tcggaaggcc ggttatgctc aactcgccgc
    17881 cggccggccg cgccggccgc atcccctcgg tcaaatgctt gagcatcgcc accgcctgga
    17941 aacccagcgc cggcgagttg tcatactcgg ccccgctctt gcgccccatg acgtaaaagg
    18001 gcgaggccga catcacccgg atgtcgtagt agccggtgcg taggatcggg ccgtccttgg
    18061 gatcctcggt accgtagggc cggttgagcc agaggttgct gacatgctgc cagagaaatt
    18121 ccccggcggt gaagccgccc aggtcgggcg agtcaatgat cagggcggtc ggccgcggca
    18181 gggtccgttg tcccaccgcc gggatatcgg cgtaccggcc ctgccagccg gccttccggc
    18241 cggcattgat catggcggcg ctgccgccgg gctggccgat gtagttgccg ctcaggaagt
    18301 cgaacttatc cgggccgacc agggggatca gtttttcgaa gaatttccag tcgccgttga
    18361 tgccgaactc catttcgcat ttgctttcgc gggccgcctt cttggccgtc gcgtaggcgg
    18421 ccgcgtagat ccgggccact tccggcagcg gcggccagcc ggtctcgtcc tccagcacca
    18481 ccgccttcac ccggccggcg tagtggcgca cgaaggcgtc aacgtaattg accaccgcct
    18541 tcgtgtaggc cgcctcgtct acgaggaggt ttttctcgac atatttggcc acataggccg
    18601 gcggcttttt gaagacctcc aggcagacgg ccggctcgac tccctggcgg atcgcctcgt
    18661 ccaggccggc ctcctcgatc caggtatatt tgcccggtgc ctgttccacg ttgttccagc
    18721 gccccggctg gctggcgctc atgatgtagg aacggctcat gcccagccgc ttcatcagcc
    18781 gggccgtgta gccgcgggtg gcgtcgccga tgttggcgca ggcgccccac ttcgggtggg
    18841 ccgcctggtt gatcaccggc aggaaggaga cggaggcgtc gcccagcagc ggggaagcct
    18901 cggcggcgca ggccagccgg tagccgttgt ggggcaggtc ggccaccggc agccgcttgc
    18961 gggtggtcgt ttgggccggg aaaacctcgt tgaactcgcc ccggcgcacc agctcctccc
    19021 ggtagttgta gatctcccag gtcacccgcg cccgccgggc cgccccgttg ttccggatcc
    19081 gcagctccac cacccgctcc tcgccgtcca cgtaggtgca ggtctcctcg ggaatgtaaa
    19141 aaccggcttc gatttcggcc gccgcttcga aggcggccgg cgcctcggcc cccttcgtca
    19201 ggctgaccgc ctcgaccagg atgtcggtgt cgggaaactc cggccgcagt tcgacgtgca
    19261 tcagcgggtt gaccgggttc tgaatcttga ccgggaaacc gacctggtgc caccggtctt
    19321 cctcggggat ttcgaagaat ttccggtaat tgtccacctg gctggcccgg tcgtaattac
    19381 ggctggtcag gaccagttcg aagcggccgg gcttcttttc gaagccgggc ccccgcttca
    19441 cccaggcggc caggtagtag tcaccatctt ccagacggta aatgcggctg cccatcggcc
    19501 gctccagttt cagcaccccg tcccgccagg aggcgcggaa gttggggccg taattgccgt
    19561 agaccacgcc ccagtaactg ttgccgtagc cgatctcgaa gccgccgtcc cagagcaggt
    19621 tgcggccggc cgccgccgaa aaagacggac tgaaaacagc ccagcctaac agcaatgcca
    19681 gtatgaaaac gaacttccgc atgacacgcc tcccggttaa aattgtctcg cggaaagaag
    19741 ccgcggccgc cggaaaaaat caaggggcag gtaacctatc attaaggatt atacaatcag
    19801 gtttcagggc caatcaagtg cggccggccc ggatcaaaag gcagctcgaa tccggccgcc
    19861 ggcccttttc ggttctccgg ggaggggggg ttagaatggt ttgctctcga gcggagaatc
    19921 cggttccagg ttccaggccg gctcctcgtc ggacatctgt atcttcggct gaagcaggac
    19981 ctttttatag ggctggggct gatgggtccg tttctccttg atgcgcgcca tcagcaggtt
    20041 gacggccgtt cggccgattt catgggtcgg ttgaatgatg taggcgaccg gggtgttcga
    20101 ctggggtacg atcatcgggc ggtcgaaaca gaccagccgt atcttccttg gcaggcggcc
    20161 ggcgaagagg ttgagaaaat tgatgagctt gatggtggtt gattcgttgc cggagaagat
    20221 gccgtccacc tcttcctgtt gaaagaaccc gatcgtctcg tccgggtcga gcggtttttt
    20281 taggggcact tcgcggaaga atgtttccgg ggcctccagg ttgtttttac gcagggcttc
    20341 ccggaagccg gccacctttt cccggctggc gtaggtcacg tactgggagg cgtaggagac
    20401 gattttccgg ctgccggacc tgaccaggtg ttcgattccc aggtaggcgc tgttgaagta
    20461 atcggtggcc accaggtcgg cggtcaggcc agacagttcg agtccggtca ggactaccgg
    20521 gatcttcttt tccaccaggt tccggtatcc gttgttcagg ttggaattat agctcggcgc
    20581 tacgatcagc ccggaaatac cgcggtcggc gaacatcgaa agatactctt tttccttttc
    20641 ggggaccccg tcgtagttgc cgagcaccag gtggaatccg ttctcccggc aggcatcttc
    20701 ggcgccgcgc acgatgccca ggtagagcga gacggtgacc gaggggatca gcattccgac
    20761 gccccggttc atcttgcgca gcttgtcccg ctgcttgcgc atgacctcca ggctctggtc
    20821 gtccggggag gtcacgaagg tgccgcgtcc cggcacctgg taaaggtatc cttcggagac
    20881 cagcagggag aggcccttgc gcagggtcat ccggctgacc ttgtactctt cggccagttc
    20941 gtactccggc gccatccggt cgcccggttt caaccggccg ctttccagct tcttgcgcag
    21001 gtcgtgttcg atgcggagat aggcgggtat attggtccgt ttcatgaata atattaaacc
    21061 ccagaacccg gtttttgtca atcaaaacaa acatatactc aaaccgggaa tgaagggctc
    21121 ttttttattg tctatgatac tgtttttatc atacaaaaat ccggttccgg gagcccggcc
    21181 ggattctttt gttctttaat aatgccggaa caagaggcgg acaatcacga ttgtcaaacg
    21241 gggctgacca gtacggacgc ttcctggtcg cccctatttt ttaagggtga cccgcccccc
    21301 gaagagaagt tccacccgcc ggttggcgta accggcctcc acccgcatcc gatggccggc
    21361 ctctttgtgc atatgcaagg aaatttgtcc aattttctcc cccttttcga gaatccgcag
    21421 gagggtgaca tagccggtcc gggtctcgtt cttgatgaag cagagtttct ggggcgccgt
    21481 cttcgcctgg cggcgaaccg gcagtccggt cgcctggatg tgggcgcaga gatgggcgtc
    21541 cgcctgggcg atcatcagcc ggggaccgcg gccgccggtg accccgagcc agaggttgga
    21601 accggcctgg acccgcttcc gggccaggcc ctggggcagg atgaagttct ggctgaaggc
    21661 gtaccaggaa tcgctttcga gccggtcgca gatgatccag gtccggtcgc cgtcgtagta
    21721 gaactggcgc gtcaggaccg gccggccgtg gaggtggtca taagcccggc tgaccgcggt
    21781 tgccaggagg ccggcgccgg cctcttccca ctgggtgacc cgggccagcg gcggctggcc
    21841 gtcctcccag ggacgatggg agatattgtg cagggagagg gtactgtgga agggggtgga
    21901 aaggaagccg agccggttgg gcgagttgtc gtagctgtaa gcgcccggat cggttatcag
    21961 caggttctgc ccggccgaga cgatcaggga gagcaggttg aagtggccgt ggaacatgcc
    22021 gtgcgggccg cagaggaaaa gggcgtaggc cgcctccttt ttccagccgg agcgcatcgg
    22081 gtagaagccg gatttcggga aggcgtaatt cagttttttc ggcggcttga tgcttgagaa
    22141 atcggccgtg acgaagcggc cgcccaggtg ccagccgagt tccggcagtt tgccgattcc
    22201 gtgcagcagg tccggccggc cgcaggtcag ggcggtcagg gccagcgaaa ggtcacgccg
    22261 gcgcaggccg gtatcgccca ccggcggcag ttcgccgccc ggcatccgga gcgccgagaa
    22321 gaactcggcc gatttttcca ggcgccggat cacccggtcc ggcagcattt tatggttgac
    22381 ccggtcctgc cgccaggcat aggcgtacca gtactggcag acgctgtggt aattcgggct
    22441 ctgctcgtaa ttgccgccgt cggagcgaaa gatgatctcg acattgtcgg ccaggaaccg
    22501 cccccactct tcccggtagc ggtccgcatc cttgaactcc gggaagagcg tcgcccagaa
    22561 gaaggagccg atggccccga ccagggtgat gttgctgaag tggaaatcgc ggcggtggcg
    22621 gtgcgtgaag gccagtttcc ggtggccggc caggagcacc cgggccagga cctcgtcctc
    22681 gatcagcccg gcggcggcgg ccaggtagag gaactcggtg gcgtaactca ggtagcggcc
    22741 gaccgtcagg ccgtcccagc cgaagccgcc gcggtgcttg ccgccgggcg gcggcggcag
    22801 ctggcagcag tggtgggcaa gcaccgcctc gccctggcgg aggtaggccg ggtcgccggt
    22861 cagggccgcc gcccagccca ggccgtgcag gtggtcgaaa cgggtatcgt tccagaaccg
    22921 ggcttcgccg gcccgggcca gcgtcagcag ttcgcgcccc agtttccggt aggcggcggc
    22981 cgccgccggc gcttcggaat tcaagcgccg ggcctcctcc cggtaatcgt cggccgtcag
    23041 gtagggccgg ggtgaaactc gttccaccag ggcctggtgc agccgccggc cggtccagcg
    23101 ctcattgagc ggccaggcgg ccaggtccag cggggccagc gaaggcgatt tcaggtcaag
    23161 ccggccgagc agttccttct tctcggcggc ggtcatccgc gtgttcatct ttaccggctc
    23221 ccgttttcaa tggacgattt ttcggtcttt ccgtttcagg cggcggagct tatcaccacc
    23281 cggctctccg gtccggccac cccgcgggtg ttgacgccgc ggaccgagag gcgcccctcg
    23341 ccccggcgcg gggtccattc cagttccgcc tcccgctggt tttcgaagcg gtatttccgt
    23401 ccgtccgcgg ccgcctcgag gtggctgaag ttcggcaggt tgtggtggaa acggaccttg
    23461 agcgactttc cgttccggag caacctgatt tcggcctggt tgatcgggta attcaggtga
    23521 tagggatcgg cctcctcgat caccatgccc ttgcagacgt aaacgttgcc gcgcccggcc
    23581 ccggtcttcg gatcgaagcg gcgccagttc cggttgcggt tgaaccggtc gcggaccatc
    23641 aggagcgggt ggatgtggtc gctgggtgga aagtcgaaga agttgttgtg gaggtagacc
    23701 acgtaccact cgaaactcca ggcgccgaag ccgccgtgga cctcgcgggg catcgggtag
    23761 cggtccggcc
//
LOCUS       DAOQYN010000004        23478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_2522_length_23478_cov_4.307518, whole genome shotgun sequence.
ACCESSION   DAOQYN010000004 DAOQYN010000000
VERSION     DAOQYN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23478
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2522_length_23478_cov_4.307518"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKN80_00335"
     CDS             <1..266
                     /locus_tag="PKN80_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34499.1"
                     /translation="LVILFFLHRMLKRFARREEEISNGVFQGLRPVVIALVAYAVFTL
                     ARGAFTGPATWLMALAAFGLFLMDETAIVPIILVFGLVGILIF"
     gene            331..771
                     /locus_tag="PKN80_00340"
     CDS             331..771
                     /locus_tag="PKN80_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNQ34500.1"
                     /translation="MNEDLLARAYRLLGRRAYTRSALLKKLREKGPAEAAEAAVEALA
                     RAGYLNDADFARDYVRQRSSNRPSGRRFLKMKLQSKGVAPEAIEAALADLDPDQEAEM
                     ARQLAEKKARTLARLEPAVRRRRLYQFLVGRGFSPDLAEKYAKL"
     gene            758..3352
                     /gene="alaS"
                     /locus_tag="PKN80_00345"
     CDS             758..3352
                     /gene="alaS"
                     /locus_tag="PKN80_00345"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNQ34501.1"
                     /translation="MRSSEIRNVFLEFFRSREHVVVPGIPLVPPEDPTLLFTSAGMVP
                     FKNFWAGNDQIPFVRAVSVQRCLRAGGKDSDLDNVGLDSRHHTFFEMLGNFSFGDYFK
                     TEAIDFAWEFLMQRLRLPESKLWVSVYQDDGEAAGIWKKYVRPERIVKLGARDNFWGP
                     AGETGVCGPCSEIYFDRGPGVGCGRPDCAPGCDCDRYLEFWNLVFPQFNKAADGTLLP
                     LARRGVDTGMGLERISALCQEAPSNFETDLFQPIINRLVELTGRAYPGRGEEQAAFRA
                     VADHVRALTFAIAENIMPANEGRGYVIRRILRRAVKFLRVLQVREPVLHRLVPEVVGL
                     MKEAYPHLAEREKFTAATIAAEEERFLELLTEGRRIFRQLTVGVRGRTVPGRTVFQLY
                     DTHGLPLETIREIAGEAKFSLDEAGFEAELENQRAASRQASAFEGVPDRTPLAGLSPT
                     RFAGYDREILETLVGAVIPLPDGSSSLVLTETPFYPERGGQLGDRGEIGGEHFKFEVR
                     ETRVDEAGFIHHLGRFSSGRAEAGVSARAVVDRGHRRRTAANHTATHLLHAALREVLG
                     DEVKQAGSLVADEYLRFDFTWNQELDRESLGRVEALVNQVIQADLSVFKEEQALADAV
                     NDGAVALFTEKYGEKVRVVTMGDFSKEVCGGVHLENTGSLGLFVISATASVGKGIRRI
                     EALVGAAAYRWLKERSDYLVELGRRLKIEPLSLAAELDRYDRERQESRVRLNAFFGAY
                     LESEVGRLEASAVEVGGRRLMVSAQPLFTAQEARLLLDRLRRDSRGAVAAVLVAGGAA
                     SQVFLGVTPDLVEQGVRAGGLLKALAARFGGGAGGRDDFAQAGIKKMIQTEELVAALK
                     AILAGGTG"
     gene            3375..4193
                     /locus_tag="PKN80_00350"
     CDS             3375..4193
                     /locus_tag="PKN80_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNQ34502.1"
                     /translation="MTPKREKRKRPPDELEIFYRFARGEVEVRDQIVMEHQALVAKIA
                     RYYARKSSATLTELMAEGYIGLLHAIEKFNPATADRKGVKFSSYAYWWIRRYVIRYLM
                     RGQSILCVPESVSELTNRYSHFSNRLAQVLGREPTVLEMKAEMELTEKEFQRVRQRRD
                     LTEISLDEKIAGDEDNRTFAEVITAGPGENLEKTLENEMILERFFSLLNPVEREVLKL
                     RYGLNNEPQCTYTEIASRLQEKFDRRLSRQRIHQIVRAAIEKLKSAHQKELNDG"
     gene            4186..4704
                     /locus_tag="PKN80_00355"
     CDS             4186..4704
                     /locus_tag="PKN80_00355"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNQ34503.1"
                     /translation="MDEKRIKEFIRSIPDFPKPGILFRDITPLLAEPDVFRDVLKQLA
                     DACPLETEVVAGIESRGFIFGAAVAARLGKGFVPIRKPGKLPYLTTSVSYQLEYGTDT
                     LEIHQDALADRRRVVLVDDLLATGGTAAAACQLLEKVGGQVLKALFLVELRDLDGRAR
                     LAGRTVEALVGF"
     gene            4759..5079
                     /gene="rpsF"
                     /locus_tag="PKN80_00360"
     CDS             4759..5079
                     /gene="rpsF"
                     /locus_tag="PKN80_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNQ34504.1"
                     /translation="MERSYEALLIIDPAISAENLQAEQTRLTGFFPEAKATCTNLGLK
                     EFTYPIKRKNQGYYLALNFTADNQQKTELENSLRLNPIVLRYLVIRALRPKKESPAKT
                     ETVV"
     gene            5102..5515
                     /gene="ssb"
                     /locus_tag="PKN80_00365"
     CDS             5102..5515
                     /gene="ssb"
                     /locus_tag="PKN80_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNQ34505.1"
                     /translation="MSVNINKVFLAGNLTRDPEMRFIPSGTAVATLRLAVNRSYTGRD
                     GNRKDEVAYVTVVVWGKQAQVCQEHLKKGSSVMIEGRLQSRSWETEEKEKRSAMEVVA
                     ERVHFLGGRRTGPGGEGSESSNTTTEEGEFEYDSA"
     gene            5502..5753
                     /gene="rpsR"
                     /locus_tag="PKN80_00370"
     CDS             5502..5753
                     /gene="rpsR"
                     /locus_tag="PKN80_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNQ34506.1"
                     /translation="MIPRKKKRERRGRRSLIARKCRFCEMGLVDIDYKDTGVLRRYLS
                     VRGKMISARFTGTCAKHQRKLSLAIKRGRYMGLLPYIIR"
     gene            5811..6275
                     /gene="rplI"
                     /locus_tag="PKN80_00375"
     CDS             5811..6275
                     /gene="rplI"
                     /locus_tag="PKN80_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNQ34507.1"
                     /translation="MRVILRTDVPKLGKTGEICEVSAGYARNFLLPQHLAYPATPGAE
                     AKVADEQRIKQRRVEKEHRSLLELAGQLRDVSLTIAVTVGAQDQLYGSVNEREIVEAL
                     KQEYSIELEEKNLKLEEPIRQLGVYRVPVRLAENVESMVTVWVVKSDGQHQS"
     gene            6256..7614
                     /gene="dnaB"
                     /locus_tag="PKN80_00380"
     CDS             6256..7614
                     /gene="dnaB"
                     /locus_tag="PKN80_00380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNQ34508.1"
                     /translation="MGSTSLDLLEERVPPNNLEAEMALLGSMLLSDDARLQAIETVRA
                     EHFYKKSHAQIFESIVRLAEANEPCDLVTLTDSLTRQKALKDLGGPAYLASLIDLVPT
                     PANIGQYIKIVREHFIRRTLIESCSQIIGKSYHLAEDIEQLMDESERAIFQISERQQS
                     DRIIPMRELVHQNFEAISRLYQNREYLTGVATGFEKLDTVTGGFQNSDLIVLASRPSM
                     GKTSLACSMVRYQVVDKKLPAAFFSLEMSRDQIVQRLLCSEARVNLSNLRRGYAQHSD
                     IPQLTLAAGKLSEAPLYIDDSPGMSALEIRAKSRRLVARHQIKIVYIDYLQLIRSRTR
                     FDNRQQEISEISAGLKSLAKELNIPVVAISQLSREVEKRDNKRPQLSDLRESGSIEQD
                     ADVVLLMVRPEFYNPVPENEGLAELIVAKQRNGPTDNFNLTFIKQYTRFENCAEREET
                     AS"
     gene            7667..8383
                     /gene="ispD"
                     /locus_tag="PKN80_00385"
     CDS             7667..8383
                     /gene="ispD"
                     /locus_tag="PKN80_00385"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNQ34509.1"
                     /translation="MVTAVIVAAGRGQRLSGRQEKPFVKLAGRPLLAYSVELFDSHPG
                     IDAVVVVAAADRLERVRRLLPTRRYRKLAALVAGGSRRRDSVAAALAVLPTGSRVVLV
                     HDAARPLASPELLSRLIKAGRRQAAVPGLPVSDAVWKAAAGRCERPVARDGMFQVQTP
                     QAFPVRVIRAAYASGRTGGGRADDDAALARRAGFPVRLLPGEPTNFKITYPADLAAAE
                     AFLRARPKPGQKGKGRCLTR"
     gene            8368..8739
                     /gene="queD"
                     /locus_tag="PKN80_00390"
     CDS             8368..8739
                     /gene="queD"
                     /locus_tag="PKN80_00390"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNQ34510.1"
                     /translation="MPYEIEVQDYFSAAHQLRGYRGKCEHMHGHNWKVAVALRAGQLD
                     KTGLVLDFTILKRLLRQALKEFDHTVINDHPEFQRQNPTSENLARFLFERLQPRFKKS
                     GLTLARVTVWESIRSRASYYE"
     gene            8736..9419
                     /gene="queC"
                     /locus_tag="PKN80_00395"
     CDS             8736..9419
                     /gene="queC"
                     /locus_tag="PKN80_00395"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNQ34511.1"
                     /translation="MSGKAVLLLSGGLDSATVLYWARRRRFEIHPLFFDYGQRHLRER
                     RAAVRLARLNGLELRVVRIRFPWGGSSLLEAGRELPDSAPEAIGRSIPSTYVPARNLV
                     FLAHALSGAEALGAGSILIGANSLDYSGYPDCRPEFYRAFNRVARLGTRAGVEGRPVR
                     VVTPLLRLSKSGIIRLGTRLGVPYQFTWSCYAGGRRPCGRCESCRLRARGFAGAGRTD
                     PLLDDGGPD"
     gene            9403..10101
                     /locus_tag="PKN80_00400"
     CDS             9403..10101
                     /locus_tag="PKN80_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNQ34512.1"
                     /translation="MAARISEVFFSIQGEGLYLGCPQVFVRFAGCNLACRYCDLVGRK
                     LPVRRLGSAGLTRSVLRLVRAAPALHSISITGGEPLLQAEFLAGWLPGLKERGLKIYL
                     ETNGTLTGALERLGPWLDFVAMDVKLPSTGGGPPLWKAHREFWLACRRLPAESFVKVV
                     VNRWTTAAETARAARLVAAAGPAGMLVIQPEVDGRGRPAIGCERLQELFRTAAAVHPG
                     CRLIPPVHQRLGLP"
     gene            10165..11181
                     /locus_tag="PKN80_00405"
     CDS             10165..11181
                     /locus_tag="PKN80_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HNQ34513.1"
                     /translation="MKNESELEDRLSEPTPEVVESVARLDGDFILLGVAGKMGVSLAR
                     MVRRALDRSGRRGRVTGVARFSDPSLREKLESAGIGTLAGDLMDRSFLELLPEARNVI
                     YMVGVKFGSTGNEPFTWASNAYLPALVAEKFRASRLAAFSTGNVYPFVPVDSGGSRET
                     DPTGPVGEYAQSCLGRERVFQYFCGRYQTPAVILRLNYAIDLRYGVLLDIAQRVFRGE
                     PLDLATGHVNFIWQGDANAFTIRSLEIAEVPPRLLNLTGPGTFRVRDLAEAFGREFQR
                     QPVFSGVESGTALLADAGQCRELFGPPRVEVETMVAWVAEWVRSGGPTWKKPTHFDTR
                     DGKF"
     gene            11181..12224
                     /locus_tag="PKN80_00410"
     CDS             11181..12224
                     /locus_tag="PKN80_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016423498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNQ34514.1"
                     /translation="MKGKTDLKSVRALLLEGQVIPAHPLALTDGLRLDERRQRALTRY
                     YLAAGAGGLAVGVHTTQFEIHDPKTGLLDPVLELAAETAAEADRPLVKVAGICGTTRP
                     AVAEAVRAAELGYQAGLLSLAALQGVSDAGLVRHCRAVAEAIPLFGFYLQPSVGGRVL
                     GYEFWRRFVEIERVVAIKVAPFNRYHTLDVVRAVAESGRTDIALYTGNDDNIVGDLLT
                     GWEFRGRRQGFAGGLLGHWSFWTRRAVELLALIKRRRAAGGPLDDLLTLGAAITDVNA
                     AVFDPAHGFRGCLSGIHLVLQRSGLLKGIRCLNRAERLSPGQRSEIDRVRRAYPDLND
                     DLFVSENLETWLR"
     gene            12221..13465
                     /locus_tag="PKN80_00415"
     CDS             12221..13465
                     /locus_tag="PKN80_00415"
                     /inference="COORDINATES: protein motif:HMM:NF010589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HNQ34515.1"
                     /translation="MTPNEKILGGINRELKRLEPELTASLMELVSIPAVSPVSGGAGE
                     MKKAIRAEALCREAGWEKIVHYDAPSPAGPRPNLVATLAGRDRRAGRTWIIAHLDVVP
                     PGDPALWQTDPYRPVRRGDRIYGRGTEDNGQSLIAALYLPKLLGRLGLQPAGDLCLAL
                     VADEETISTYGIKYMAGRKSLFRPGDFFIVPDGGSPDGSLIEVAEKALVWLEVRTLGR
                     QCHGSMADQGINAMEAASVLVSRLERLRRRFAGRDPLYSPDFSSFEPTKRLANVPNIN
                     TIPGSDTFYIDCRILPRHPVPKVMGELKKLKAQVEKERRVKISLSFVQRAPATGTDPE
                     APEIKEFARTVRAASGVRTRVAGVGWGTCASFVRQAGYPAAVWSTVLGIAHQPNEYCL
                     VSNLLNDARVFAGYLAGLGRQP"
     gene            13462..13989
                     /locus_tag="PKN80_00420"
     CDS             13462..13989
                     /locus_tag="PKN80_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNQ34516.1"
                     /translation="MKVLVVIAREGFRDEEYFKPKAALEAAGHLVVTAAGQAGPARGK
                     LGGQAAAEISLAEAEIGDYAGLVFVGGPGATGYFEDSRALDLAGRAWSRGLVVAAICI
                     APGILARAGVLAGRRATVFPGEEIDVLKAAGAEYQDRPVVVDGRLITANGPEAADDFG
                     RALVRALADQAAGRK"
     gene            13986..14957
                     /locus_tag="PKN80_00425"
     CDS             13986..14957
                     /locus_tag="PKN80_00425"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNQ34517.1"
                     /translation="MKRFLGVAVRCLVSGLLIFILAVPASRFISPRLPLVDITALPAT
                     LAAADPAALAGAVLLALASVLFIAGRWWLLVGSRSVPYHFLLRLTFIGIFFNNFLPTG
                     AGGDLVKGYYLLHGRSGKLDLGLSIVMDRLVGVFSIMTLGYLAVIVNFDRLPGAAAWT
                     VTAIFLALLFFLLLLAWRGLAVRLARRLGLEGWGRFGALLKRVYQGGRAYLEEPARLL
                     GSLACSFGSQGLQLAAFYLVARALGTPVTFVSLCAYVPLVWSFALVPSLGGLGARETA
                     YTLFFQGQMGRENALALGLIILGLTLTQSLVGGLLYFLGGGRWKTAS"
     gene            14939..16321
                     /locus_tag="PKN80_00430"
     CDS             14939..16321
                     /locus_tag="PKN80_00430"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNQ34518.1"
                     /translation="MENGKLTYERTLVWDNLSEAEKKEVFAFAGAYRAFLSEVKTERE
                     FIDRTVSMARARGFREPGGRRASDRVCLVNKNATVGLLVRGRRPFKDGFHLVAAHIDA
                     PRLDLKPQPLVEDEGLALLKTHYYGGIKKYQWVTRSLSLHGVVALKNGECRKVTLGEA
                     ADEAVFTVTDLLPHLAKDQMERKMSEAVQAEALNLLIGSLPAEAKKADQKKAPGRVKE
                     AILGRLRAKYGFTEEDFISADLQIVPAGPARDLGLDGSCILGYGQDDRICAYTALKAV
                     LDLDEPEYTAVCLLFDREEIGSEGVGSAQSVFLRVFLAEASGLQGLELDRVCGRSYAL
                     SADVSAGLDPSYKDVSEPQNSPRLGYGTVMTKYTGWAGKYSASEASAEYVAYLRRLFE
                     KHRVFWQTGELGKVDKGGGGTVAKYLARQNIETIDIGPALLSMHSPQEVSHKADLWSS
                     YRSFLAFYRG"
     gene            16343..17449
                     /locus_tag="PKN80_00435"
     CDS             16343..17449
                     /locus_tag="PKN80_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34519.1"
                     /translation="MADGRKRLAELADWYRDRPLDRDLEARVLSELGLSLAARYGGQT
                     LKNPLLVAPGQLTLTLSQVQAIRKAGYAGCVLKSVVGESPDGACSMARQRIKPTSVRT
                     YYEPEDTAGAFPIIHWDGRLDIRGLDEYLDFAGAAAAEDSYGSFSVVASLLCHLPRPG
                     EDFRADEWSHTAGRLGQLGYRHLEIDFCPFLSSDRYTEDQANILRWYRSVPALVKRAG
                     PSLRVFPKLLNLDWGIDYQVQMARAAVEGGADGLVVANRGYRPEFASGHGGPGLRRLN
                     LEQVRRIKREFPGLPVSATGGVYRGRDVLEYLEAGAENVQLLSYLMGRVKAPFRRGTG
                     NRLEKVLYQLIFDPDDGLLAVWLDGPKRAGGMTK"
     gene            17446..18210
                     /locus_tag="PKN80_00440"
     CDS             17446..18210
                     /locus_tag="PKN80_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HNQ34520.1"
                     /translation="MNRVACVTGAAQGIGLAVARELAGLDFRVLAVDLRPAGEGAGVI
                     GGLPGADHRYLAADISAAAGREAILRLLREELGRLDLLVNNAGVAPRERRDLLETSEE
                     SFDRVLNINLRGTFFLTQAAARFMVELAAGGAGAGKIVNISSMSAYTSSVNRPEYCLS
                     KAGVSMLTRLFADRLAEYGINVYEIRPGIIRTPMTEAVQARYDDLINNRGLLPIRRWG
                     RPEDVAAAVRAIAQDAFPYSTGQVFDVDGGFHLRRL"
     gene            18247..20238
                     /locus_tag="PKN80_00445"
     CDS             18247..20238
                     /locus_tag="PKN80_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNQ34521.1"
                     /translation="MTKSSLNFEPGRSAPLYRCRVLVVGSGAAGLNCAEQLRLAGVDD
                     VAVISTRRLGGTSYRSGSDKQTYYKLSNFGDTLDGPMEMAHSLFDGGAMDGGLAYIES
                     LYSLPAFYHLVEIGVPFPSNRYGAFVGYKTDHDPRQRATSAGPKTSRYMVEKELLRLK
                     SLGVPVLDGLTAVRLVTAGRRVLGLVALDENRSEDDPALGLTVFQAGAVVLATGGPGE
                     LYAVSVYPAGQVSLHGAALEAGAAAVNLGESQFGLASTRFRWNLSGTYQQVIPAYFSL
                     GPDGRRRDFLAGWYRSLPELAGAVFLKGYQWPFHVNRLDNFGSSLVDLAVGAETESGR
                     RVFLDFTRNPARPGESFDLDSLPPEALNYLNRSGARQADPYRRLRHMNPESIAIYAEH
                     GIDLREPLEAAVCFQHNNGGLKVNADWETDLAGLFAAGELAGTHGVTRPGGAALNSGQ
                     VGGFRIARCLAGRRDELAGRRPAAPFQAALREVYRTSRAMLASDREQGRWRRLIQERM
                     SAAAGFRREPERVRAARREAEADRRRLESAPPGAAGRRNLARAWETRNLVLTQLAFLK
                     SIDAYLQSGGGSRGSGLVLAEAAVPGARRVELVGGRSLCFRPERPGDRRLRVAVSGPA
                     FTTSLEEVAPLPRDESWFETVWADWKAGRTCGGGKRRRR"
     gene            20245..21429
                     /locus_tag="PKN80_00450"
     CDS             20245..21429
                     /locus_tag="PKN80_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ34522.1"
                     /translation="MEKGELSIRNGRCLLADGRLAATDLRLSGGRIAEIGSGLKSGRE
                     LDAAGGLLLPGLVDLHTHGLEFALAETAGLDEYARLEAARGVTAFCPTFFNAPENLLE
                     TLRRHCRETGRLQAVPQAVGFRLESPYLTRTGGGRSDDLVPITTGLTDALLEAGGGLI
                     KVWDIAPELEGAVELIGRLSSRGIVCSLGHTNAGLETAAAAVEAGARLVTHLFNVMPP
                     PDPAEPGLTPVGLADYFLVEPRLWAEIIPDGTHVAPILVREALRCKGPDRLIFITDSN
                     CGAGLAPGDYLFPNRWGRVRIGGSEAGVRLIDRDLVLAGSALTALDAFRAAVNRFGQD
                     LAAASRLCSTNPARLLGLNKGEIAPGRDADLVILDDRLDLICTVAAGRVVYERAPAAG
                     IA"
     gene            21445..22380
                     /locus_tag="PKN80_00455"
     CDS             21445..22380
                     /locus_tag="PKN80_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34523.1"
                     /translation="MSKFQYEPARCVPFRDRAVLERCRRIRAAAITRHPNPDFRIRVV
                     PDAKITRLWLVDMFERIRTAAEAGRPLILITPQPWPDGYRELAGLINRHRVNCKKLHT
                     FNMDEYANEKGETAPETWPAGFLYSMLNNFYRRLDPKLRPPRRQVQGPTHRNIRDYGK
                     MIADLGGADCCYSGPGWTGHLAFIEPDAPEFRGGLAAWKKMGPRLVTLSPFTIAQNSL
                     HAAFGMSGDLAAVPPQAATIGPAEVIAARFRFDMHAITVGGSRVSWQRLMTRLCLHGP
                     VTPKLPQSILQTLRTDVYVSESIAADIEPDYDGGY"
     gene            22407..23027
                     /locus_tag="PKN80_00460"
     CDS             22407..23027
                     /locus_tag="PKN80_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNQ34524.1"
                     /translation="MKETLERIQKGRVIAVLRGDTVEKTVRTAEALVEGGVNLIEITY
                     TMDDPLAAIERLAGRDDILLGAGTVLSLKDARAARAAGARFLVSPCLIPEVVGYGREE
                     NLLVMPGIFTPTEAFQALSLGAEVLKLFPGSLGGPDLIKAFRGPFPGLKLVPTGGVEK
                     ENLRAWFAAGALAVGMGGNLAPREAIQREDYASIREKAREIMGLLP"
     gene            23024..>23478
                     /locus_tag="PKN80_00465"
     CDS             23024..>23478
                     /locus_tag="PKN80_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015591057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNQ34525.1"
                     /translation="MSGEESLEIKGVRVTVVQGDLTAQEVTAIVNAANNKFLMGGGVA
                     GAIRKRGGAEIEAEAVRLGPKPVGEAVVTSAGRLPCRHVIHAATMAMDFQTDETKIRM
                     ATRNALKRVRENGMEILAFPALGCGVGGFPVDRAARIMAEETAAFLVEGS"
BASE COUNT         3674 a         8086 c         7974 g         3744 t
ORIGIN      
        1 cgctggtcat cctcttcttt ctgcaccgga tgctgaagcg gttcgcccgg cgcgaggagg
       61 agatatccaa cggggtcttc cagggcctgc ggccggtggt gatcgccctg gtcgcctacg
      121 cggtcttcac cctggcccgg ggcgccttta ccggcccggc cacctggctg atggccctgg
      181 cggccttcgg cctcttcctg atggacgaaa cggccatcgt gccgattatc ctggtcttcg
      241 ggctggtcgg gatcctgatc ttctgaggcg gcccccggag gccggggttg attgccgggc
      301 ggcggcccgc ggtgttaaga tttaaggacg atgaacgaag acctgctggc ccgggcttac
      361 cgactgttgg gccgccgggc ctacacccgg agcgcgctgc tcaaaaagct ccgggagaag
      421 gggccggccg aggcggccga agcggccgtc gaggcgctgg cccgggccgg ctacctgaac
      481 gacgccgatt tcgcgcgcga ttacgtgcgg cagcgaagtt caaaccgtcc ctccggccgc
      541 cggtttttga agatgaagct gcagtccaag ggcgtggcgc ccgaggcgat cgaggcggcc
      601 ctggcggatc tggatccgga ccaggaggcg gaaatggccc gccaactggc cgagaagaag
      661 gcccggacgc tggcccggct ggagccggcg gtccggcggc gccggcttta ccagttcctg
      721 gtgggccggg gcttttcacc tgacctggcg gagaaatatg cgaagctctg aaatccggaa
      781 cgtttttctc gaattcttcc gttcccggga acacgtggtg gtccccggca ttccgctggt
      841 gccgcccgag gatcccaccc tgcttttcac ttcggccggc atggtgccct tcaagaattt
      901 ctgggccggc aatgaccaga tacccttcgt ccgggccgtc tcggtgcagc gctgcctgcg
      961 ggccggcggc aaggacagcg acctggacaa cgtcggactc gactcccgcc accacacctt
     1021 tttcgagatg ctgggcaact tctccttcgg cgattacttc aagaccgagg cgatcgattt
     1081 tgcctgggaa ttcctgatgc agcgcctgcg cctgccggaa tcgaaactct gggtttcggt
     1141 ctaccaggac gacggcgaag ccgccgggat ctggaagaag tacgtccggc cggaacggat
     1201 cgtgaagctg ggcgcccggg acaacttctg gggtccggcc ggcgaaaccg gcgtctgcgg
     1261 tccctgctcc gagatctatt tcgaccgcgg cccgggcgtc ggctgcggac gcccggattg
     1321 cgcgcccggc tgcgactgcg accgttacct ggaattctgg aacctggtct ttccgcagtt
     1381 caacaaggcc gccgacggca ccctgctgcc cctggcccgc cgcggggtcg acaccgggat
     1441 ggggctggaa cggatcagcg ccctctgcca ggaggcccct tccaacttcg aaaccgacct
     1501 tttccagccg atcatcaacc gcctggtcga attgaccggc cgcgcctacc cgggccgggg
     1561 cgaagagcag gcggccttcc gggcggtcgc cgaccacgtc cgggcgctga ccttcgccat
     1621 cgccgaaaac atcatgccgg ccaacgaggg ccggggttac gtcatccggc gcatcctgcg
     1681 ccgcgccgtc aagttcctgc gggtcctgca ggtcagggaa ccggtcctgc accggctggt
     1741 gcccgaggtg gtcggcctga tgaaggaggc ctatccgcac ctggccgaac gggaaaagtt
     1801 cacggccgcg accatcgccg ccgaggagga gcggttcctg gaactgctga ccgaggggcg
     1861 gcgcatcttc cgccagttga ccgtcggcgt ccggggccgc acggtacccg gccggaccgt
     1921 ttttcaactc tacgataccc acggcctgcc gctggagacc atccgggaga tcgccggcga
     1981 ggcgaagttc agcctggacg aggccggttt cgaggccgaa ctggagaacc agcgggcggc
     2041 cagccggcag gccagcgcct tcgagggcgt cccggaccgg accccgctgg cgggcctctc
     2101 tccgacccgg ttcgccggtt acgaccggga gattctcgaa acgctggtgg gcgcggtcat
     2161 tccgcttccc gacggttcca gttcgctggt cctgaccgag acccccttct acccggagcg
     2221 gggcggtcag ctgggcgacc ggggcgagat cggcggcgaa catttcaaat tcgaggtgcg
     2281 cgaaacccgg gtggacgagg ccggttttat tcaccacctg ggccgttttt cgagcggccg
     2341 ggccgaggcg ggcgtgagcg cccgggcggt ggtggaccgg ggtcaccgcc ggcggacggc
     2401 ggccaatcat accgccaccc accttctgca tgccgccctg cgcgaggtgc tgggcgatga
     2461 ggtcaagcag gctggctccc tggtggcgga cgagtacctc cgttttgatt tcacctggaa
     2521 ccaggagctg gaccgggaga gcctgggacg ggtcgaggcg ctggtcaacc aggtcatcca
     2581 ggccgatctg tcggtcttca aggaggagca ggccctggcc gacgcggtga acgacggcgc
     2641 ggtggccctc ttcaccgaga aatacggcga gaaggtgcgg gtggtgacca tgggcgattt
     2701 cagcaaggag gtctgcggcg gcgtgcacct ggagaacacc ggttccctcg gcctcttcgt
     2761 gatcagcgcc accgcctcgg tcggcaaggg catccgccgc atcgaggccc tggtcggcgc
     2821 ggccgcctac cgttggctga aggagcggag cgactacctg gttgaactgg gccgccgcct
     2881 gaagatcgag ccgctttcgc tggccgccga gcttgaccgg tacgaccggg agcggcagga
     2941 aagccgggtc cggctcaacg ccttcttcgg ggcctacctg gaatcggagg tcggccgcct
     3001 ggaagcatcg gccgttgagg tcggcggccg gcgcctgatg gtctccgccc agcctctctt
     3061 caccgcccag gaggcccgcc tgctcctgga ccgcctgcgc cgcgacagcc gcggcgcggt
     3121 tgcggccgtg ctggtggccg gcggcgcggc ctcccaggtt ttcctgggcg tgaccccgga
     3181 cctggtcgag cagggcgtcc gggccggcgg ccttctgaag gctctggccg cccggttcgg
     3241 cggcggcgcc ggcggccggg acgattttgc ccaggccggc atcaagaaga tgatccagac
     3301 cgaggaactg gtggccgccc tcaaggcaat cctggccggc gggacgggct gatctcaagg
     3361 gcggcgattc gaggatgact ccgaaacggg agaagcggaa acggccgccg gacgaactgg
     3421 agatttttta ccggttcgcc cggggcgagg tcgaggtccg cgaccagatc gtcatggagc
     3481 accaggcgct ggtggccaag atcgcccgct actacgcgcg caagtccagt gccaccctga
     3541 ccgagttgat ggccgaaggg tacatcggcc tgctgcacgc catcgagaag ttcaatccgg
     3601 ccaccgccga ccgcaaggga gtcaagttca gttcctatgc ctactggtgg atacgccgct
     3661 acgtcatccg gtacctgatg cgcggccagt cgatactttg cgtgcccgag agcgtttcgg
     3721 agctgaccaa ccgctactcc cacttctcca accggctggc ccaggtgctg ggccgcgagc
     3781 cgaccgtcct ggaaatgaag gcggagatgg agttgaccga gaaggaattt cagcgcgtca
     3841 ggcagaggcg cgacctgacc gagatctccc tggatgaaaa gatcgccggc gacgaggaca
     3901 accggacctt cgccgaggtc atcacggccg ggccgggcga gaacctcgag aagacgctgg
     3961 agaacgagat gatcctggaa aggttcttct ccctgctcaa cccggtggag cgggaggttc
     4021 tgaaactccg gtacgggctg aacaacgaac cgcagtgcac ctataccgag atcgcctcga
     4081 ggctccagga gaagttcgat cggaggctga gccgccagcg catccaccag atcgtccggg
     4141 cggccatcga aaagctgaag tccgctcacc aaaaggagtt aaacgatgga tgagaagaga
     4201 atcaaggagt tcatccggag catcccggac ttccccaagc cgggcatcct cttccgggat
     4261 atcacgcccc tgctggccga accggacgtc ttccgggacg tgctgaagca gctggccgac
     4321 gcctgcccgc tcgagaccga ggtggtggcc gggatcgagt cgcgcggttt tatcttcggc
     4381 gcggcggtgg cggcccggct gggcaaggga ttcgttccca tccggaagcc cggcaagctg
     4441 ccctacctga ccacctcggt cagctaccag ctcgagtacg ggaccgacac gctcgagatt
     4501 caccaggacg ccctggctga ccgccgccgg gtggtgctgg tggacgacct gctggccacc
     4561 ggcggcacgg cggccgccgc ctgccagctg ctggaaaagg tgggcggcca ggttctcaag
     4621 gcgctcttcc tggtcgagtt gcgcgacctc gacggccggg cccggctggc cggccggaca
     4681 gtcgaggcgc tggtcgggtt ttgatttcgc ggccggggtt ggggtataat aatattttat
     4741 caattatcag gaggcgggat ggaaagaagt tacgaggcgc ttttaatcat tgacccggcg
     4801 atctccgcgg agaacctcca ggccgaacag acccggctga ccggtttctt tccggaggcc
     4861 aaggccacct gcaccaatct cggcctgaag gaattcacct acccgatcaa gcggaagaac
     4921 caggggtatt acctggccct caattttacg gccgacaatc agcagaagac ggaactggag
     4981 aattccctgc gtttgaatcc gatcgtgctg cggtacctgg tgatccgggc gctccgcccc
     5041 aagaaggagt ccccggccaa gaccgagacc gtcgtctgac cgccaacgga ggaagaatca
     5101 gatgtccgtc aatatcaaca aggttttcct ggccggcaac cttacccgcg acccggagat
     5161 gcgcttcata cccagcggga ccgcggtggc caccctgcgc ctggcggtca accgcagcta
     5221 cacgggccgg gacggcaacc ggaaggatga ggtggcctac gttacggtgg tggtctgggg
     5281 caagcaggcc caggtctgcc aggagcacct gaagaagggc agttcggtga tgatcgaggg
     5341 gcggcttcag tcccgcagct gggagaccga ggagaaggag aagcggagcg ccatggaagt
     5401 cgtcgccgag cgggtccatt tcctcggcgg ccggcggacc ggtcccggcg gcgaggggag
     5461 cgaatcctcc aatacgacga cagaggaagg ggagtttgaa tatgattccg cgtaaaaaga
     5521 aacgagaacg ccggggacgg cggagcctga tcgcccggaa atgccggttc tgtgaaatgg
     5581 gcctggttga cattgactac aaggataccg gcgtgctgcg ccgctacctt tcggtgcgcg
     5641 gcaagatgat cagcgcgcgg ttcaccggca cctgcgccaa gcaccagcgc aaactcagcc
     5701 tggccatcaa gcgcggccgt tacatgggat tgttgcccta tatcatccgc taagcaggga
     5761 cggacgcgac ccgttgcgag ccttaacccc cataaaaagc agagcgaaac atgagagtca
     5821 ttctaagaac cgatgtgccg aaactcggca agaccggcga gatctgcgaa gtttccgccg
     5881 ggtacgcccg caacttcctg cttccccagc atctggccta tccggccacg ccgggcgcgg
     5941 aggcgaaggt ggccgacgag cagcggatca agcagcgccg ggtggagaag gaacaccgca
     6001 gcctcctgga gctggccggc cagctgcggg acgtttccct gaccatcgcg gtgacggtcg
     6061 gcgcccagga ccagctgtac ggttcggtaa acgagcgtga gatagtcgag gccctgaagc
     6121 aggaatactc aatcgaactc gaagagaaga atctgaaact ggaagagcct attcgccaac
     6181 tcggcgtcta ccgtgtcccg gtccgtctgg cagagaacgt ggagagcatg gtaaccgtgt
     6241 gggtggtgaa gagcgatggg cagcaccagt cttgacctgc tcgaggaacg agttcctccc
     6301 aacaacctgg aagccgagat ggcgcttctg ggttcgatgc ttctttccga cgacgcccgg
     6361 ctccaggcga tcgagaccgt ccgcgccgag cacttctaca agaagagcca cgcccagatt
     6421 ttcgagtcca ttgtccgcct cgccgaggcg aacgaaccct gcgacctggt caccctcacc
     6481 gatagcctca cccgccagaa ggccctcaag gacctgggcg gcccggccta cctggccagc
     6541 ctgatcgacc tggtgcccac cccggccaat atcggccagt acatcaagat cgtccgcgag
     6601 cacttcatcc ggcggaccct gatcgagagc tgctcccaga tcatcggcaa gagctatcac
     6661 ctggccgagg acatcgagca gctgatggac gagtcggaac gggccatctt ccagatttcc
     6721 gaacggcagc agagcgaccg tatcatcccg atgcgggagc tggtccacca gaatttcgag
     6781 gccatctcgc ggctttacca gaaccgcgag tacctgaccg gcgtcgccac cggcttcgag
     6841 aaactggaca cggtgaccgg cggtttccag aactcggacc tgatcgtcct ggcctcccgc
     6901 ccctcgatgg gcaagacctc gctggcctgc agcatggtcc gctaccaggt ggtggacaag
     6961 aaactgccgg cggccttctt ctccctggag atgagccggg accagattgt ccagcggctg
     7021 ctctgctccg aggcccgggt caacctgagc aacctgcgcc ggggttatgc ccagcactcc
     7081 gacatcccgc agctgacgct ggcggccggg aaactcagcg aggccccgct ttacatcgac
     7141 gattcgcccg gcatgtcggc cctcgagatc cgggccaaga gccgccggct ggtggcccgc
     7201 caccagatca agatcgtcta catcgattac ctgcagctca tccgcagccg gacccggttc
     7261 gacaaccggc agcaggagat ctcggagatc tcggccggcc tgaagagcct ggccaaggaa
     7321 ttgaacattc cggtggtggc catctcccag ctctcgcgcg aggtcgagaa gcgggacaac
     7381 aagcggccgc agctctccga cttgcgcgaa agcggctcga tcgagcagga cgccgacgtg
     7441 gtgctgctca tggtccggcc ggagttctac aacccggtcc cggagaacga ggggctggcc
     7501 gaactgatcg tcgccaagca gcgcaacggt cccaccgaca acttcaacct gaccttcatc
     7561 aagcagtaca cccgtttcga aaactgcgcc gaacgggagg agacggcttc ctgacggccc
     7621 ccggccagcg cggcggccgg actttcctgc cgatcccttg tcagacatgg taaccgctgt
     7681 catcgtggcc gccgggcggg gtcagcgcct gtcgggccgc caggaaaaac ccttcgtgaa
     7741 actggcgggc cgccccctcc tggcctacag cgtcgaactc ttcgattccc atcccgggat
     7801 tgacgcggtg gtggtggtgg ccgccgccga ccgcctggag cgggtccggc gcctgctgcc
     7861 cacccgccgc tatcggaaac tggccgcgct ggtggccggc ggttcgcgcc gccgcgactc
     7921 ggtggccgcc gccctggccg tcctgccgac cgggagccgg gtggtcctgg tgcacgacgc
     7981 cgcccggccg ctggcttcgc cggagctgct gagccggctg atcaaggccg gccgccgcca
     8041 ggcggcggtg cccggactgc cggtctccga cgccgtctgg aaggcggccg ccggccgctg
     8101 cgaacggccg gtggcccggg acgggatgtt ccaggtgcag acgccccagg ccttcccggt
     8161 ccgggtcatc cgggccgcct acgcttccgg ccggaccggc ggcggtcggg ccgacgacga
     8221 cgcggccctg gcccggcggg ccggtttccc ggtgcgcctg cttccgggcg agccaaccaa
     8281 cttcaagatc acctatccgg ccgacctggc cgcggccgag gcgttcctgc gcgcccggcc
     8341 gaagcccggc caaaagggga aggggcgatg ccttacgaga tagaggtcca ggattacttc
     8401 agcgccgcgc accagctgcg ggggtaccgg ggcaagtgcg agcacatgca cggtcacaac
     8461 tggaaggtgg ccgtggcgct gcgcgccggg cagctcgata agaccggcct ggtgctggac
     8521 ttcaccatcc tgaagcggct cctccgccag gcgctcaagg aattcgacca cacggtgatc
     8581 aacgatcacc cggaattcca gcgccagaac cccacctcgg agaacctggc ccgcttcctc
     8641 ttcgaacgcc tccagccccg cttcaaaaaa tccggcctga cgctggcccg ggtcacggtc
     8701 tgggaatcga tccggagccg ggccagttac tacgaatgag cgggaaagcg gtccttctgc
     8761 tctccggcgg cctggattcg gccacggtgc tttactgggc ccggcgccgc cgttttgaaa
     8821 tacaccctct cttcttcgat tacgggcagc gccatctccg ggaacgccgg gccgcggtcc
     8881 ggctggcccg cctgaacggc ctggaactcc gggtggtccg gatccggttt ccctgggggg
     8941 gaagcagcct gctcgaggcc ggccgggaac tcccggattc cgcgcccgag gcgatcggcc
     9001 gctcgattcc atccacctac gtgccggctc gcaacctggt ctttctcgcc cacgcgcttt
     9061 ccggtgccga ggcactgggc gccgggagca tcctgatcgg ggccaacagc ctcgactact
     9121 ccggttaccc ggactgccgg ccggaatttt accgggcctt caaccgggtg gccaggctgg
     9181 gcacccgggc cggcgtggag ggaaggccgg tccgggtggt caccccgctg ctccgcctct
     9241 ccaagtccgg aatcatccgg ctgggcaccc ggttgggtgt cccctaccag tttacctggt
     9301 cctgctacgc cggcggccgg cggccctgcg gccgctgcga aagctgccgg ctccgcgccc
     9361 ggggtttcgc cggcgccggc cggaccgacc cgctgctcga cgatggcggc ccggattagc
     9421 gaggtctttt tttccatcca gggcgagggc ctctacctgg gctgccccca ggtcttcgtc
     9481 cgcttcgccg gctgcaatct cgcctgccgc tactgcgacc tggtcggccg gaagctcccg
     9541 gtccggcggc tcggaagcgc cgggctgacc cggagcgtgc ttcggctggt ccgcgccgcg
     9601 cccgcccttc actccatctc gatcaccggc ggcgagccgc ttctgcaggc ggagttcctg
     9661 gccggctggc tgccgggcct caaggaacgg ggtttgaaga tctacctcga aaccaacggc
     9721 accctgaccg gggcgctcga gcggctcggc ccctggctcg atttcgtggc catggacgtg
     9781 aaactgccct ccaccggcgg cggtccgccc ctctggaagg cgcatcggga attctggctg
     9841 gcctgccgcc gcctgccggc cgaatccttc gtcaaggtgg tggtcaaccg gtggaccacg
     9901 gctgccgaga cggcccgggc ggcccgcctg gttgccgcgg ccggtccggc cgggatgctg
     9961 gtcatccagc ccgaggttga cggccgcggc cggccggcga tcggttgcga acggcttcag
    10021 gaactcttcc ggacggcggc cgccgtccat cccggctgcc ggctgatacc gccggttcac
    10081 cagcggctcg gccttccctg attttatggt aaaatattag aaaaatcggg attccaccgc
    10141 gtacttcaaa cattttccca gatgattaaa aacgagtccg aactggaaga ccggctttcc
    10201 gagcccaccc cggaagtggt ggagagcgtg gcccggctgg acggcgattt tatcctgctg
    10261 ggagtggccg gcaagatggg cgtctccctg gcccggatgg tgcggcgcgc cctggaccgc
    10321 tccggccgcc gcggccgggt gaccggggtc gcccgtttct ccgacccgtc cctgcgcgag
    10381 aaactggagt cggccggcat cggcaccctg gccggcgacc tcatggaccg ttccttcctg
    10441 gaattgctgc cggaggcccg caacgtcatc tacatggtgg gcgtcaagtt cggctccacc
    10501 ggcaacgagc cgtttacctg ggccagcaac gcctacctgc cggcgctggt ggccgaaaaa
    10561 ttccgcgcca gccggctggc ggccttttcg accgggaacg tttacccttt cgtgccggtt
    10621 gattccggcg gcagccggga gaccgacccg accggcccgg tgggcgagta cgcccagtcc
    10681 tgcctgggcc gggaacgcgt cttccagtac ttctgcggcc gctaccaaac cccggcggtc
    10741 atcctccgcc tcaactacgc gatcgacctg cgttacgggg tgctgctgga catcgcccag
    10801 cgggtcttcc gcggggaacc gctggacctg gcgaccggtc atgtcaactt catctggcag
    10861 ggtgatgcca acgcctttac catccggtct ctcgaaatcg ccgaggtgcc gccccgactc
    10921 ttgaacctga ccggtcccgg caccttccgg gtccgcgacc tggccgaggc cttcggacgg
    10981 gaattccagc gccagccggt attcagcgga gtcgaatccg gcaccgccct gctggccgac
    11041 gccggccagt gccgggaact cttcggaccg ccccgggtcg aggtcgagac gatggtcgcc
    11101 tgggtggccg agtgggtccg ctccggcggt ccgacctgga agaagccgac ccacttcgac
    11161 acccgcgacg ggaagttctg atgaaaggaa aaacggactt gaaatcggtg cgcgccctgc
    11221 tgctggaagg gcaggtgatc ccggcccatc ccctggccct gaccgatggc ctgcggctgg
    11281 acgaacggcg ccagcgcgcc ctgacccggt actacctggc ggccggcgcc ggcggcctgg
    11341 ccgtgggcgt ccacaccact cagttcgaga ttcacgatcc gaagaccggc ctgctggacc
    11401 cggtcctgga actggccgcc gagaccgcgg ccgaagcgga ccggccgctg gtcaaggtgg
    11461 ccggcatctg cgggaccacc cggccggcgg tggccgaagc ggtccgggcg gccgagctcg
    11521 ggtaccaggc cgggctgctt tcgctggcgg cgcttcaagg ggtcagcgat gccgggctgg
    11581 tcaggcactg ccgggcggtg gccgaggcca tcccgctctt cggcttttac ctgcagccct
    11641 cggtcggcgg ccgcgtcctc ggctacgagt tctggcgccg gttcgtcgag atcgagcggg
    11701 tggtcgccat caaggtggcc cccttcaacc gttaccacac tctggacgtg gtccgggcgg
    11761 tcgccgaatc cggccgcacc gacatcgccc tctacaccgg caacgacgac aacatcgtcg
    11821 gcgacctgct caccggctgg gagttccggg ggcggcggca gggcttcgcc ggcggtctgc
    11881 tcggccactg gtccttctgg acccggcggg cggtggaatt gctggcgctc atcaagcggc
    11941 ggcgcgccgc cggcggtccc ctggacgacc tgctgacgct gggcgcggcc atcaccgacg
    12001 tcaacgcggc cgtctttgat ccggcccacg gattccgcgg ctgcctttcc ggcatccacc
    12061 tggtgctgca gcggtccggc ctgctgaagg gaatccggtg tctgaaccgg gccgagcggc
    12121 tttcacccgg ccagcggagc gaaatcgacc gggtcaggcg cgcttacccg gaccttaacg
    12181 acgatctctt tgtatccgaa aacctggaga cctggttgag atgaccccga acgaaaagat
    12241 cctgggcgga atcaatcgcg agttgaagcg gctggaaccg gagctgacgg ccagcctgat
    12301 ggagctggtt tcgatcccgg ccgtcagccc ggtctccggc ggcgcgggcg aaatgaagaa
    12361 ggcgatccgg gccgaggccc tctgccggga ggccggctgg gagaagatcg ttcactatga
    12421 cgcccccagc ccggccgggc cccggccgaa cctggtcgcc accctggccg gccgcgaccg
    12481 ccgggccggc cggacctgga tcattgccca cctggacgtg gtgccgccgg gcgacccggc
    12541 cctctggcag accgatccct accggccggt gcgccgcggc gaccggatct acgggcgcgg
    12601 caccgaggat aacggccagt cgctgatcgc cgccctctac cttccgaaac tgctcggccg
    12661 cctcggcctg cagccggccg gcgacctctg cctggccctg gtcgccgacg aggagaccat
    12721 cagcacctac ggcatcaagt acatggccgg tcgcaagagc ctcttccggc cgggcgattt
    12781 cttcatcgtt cccgacggcg gcagcccgga cggctcgctc atcgaggtgg ccgaaaaggc
    12841 cctggtctgg ctggaggtgc gcaccctggg ccgccagtgc cacggctcga tggccgacca
    12901 gggcatcaac gccatggagg cggcctcggt cctggtcagc cggctggagc ggctgcgccg
    12961 ccgtttcgcc ggccgcgacc cgctctatag cccggatttc tcctcctttg agccgaccaa
    13021 gcggctggcc aacgttccca acatcaacac cattcccggc agtgacacct tctacattga
    13081 ctgccgcatc ctgccgcgcc acccggttcc gaaggtgatg ggcgagctga agaagctgaa
    13141 ggcgcaggtg gaaaaggagc ggcgcgtcaa gatcagcttg agctttgttc aacgcgcccc
    13201 ggccacgggc accgatcccg aggccccgga gataaaggag ttcgcccgga ccgtccgggc
    13261 ggccagcggg gtcaggaccc gggtggccgg cgtcggctgg ggaacctgcg cctccttcgt
    13321 ccggcaggcc ggctacccgg cggcggtctg gtcgaccgtg ctcggcattg cccatcagcc
    13381 gaacgagtat tgcctggtct ccaacctgct gaatgacgcc cgggtctttg ccggctacct
    13441 ggccggactg gggaggcagc cgtgaaggtg ctcgtggtga tcgcccgcga gggcttccgc
    13501 gacgaggagt acttcaagcc gaaggccgcc ctggaagcgg ccggccacct ggtggtgacc
    13561 gcggccggcc aggccggccc ggcccggggc aagctgggcg gccaggcggc cgccgaaatt
    13621 tcgctggccg aggcggagat cggcgattat gccgggctgg tctttgtcgg cggccccggc
    13681 gccaccggct acttcgagga cagccgggcg ctggacctgg ccggccgggc ctggagccgg
    13741 ggactggtgg tggcggcgat ctgcatcgcg cccgggatac tggcccgcgc cggcgtgctg
    13801 gccggccgcc gggccacggt ctttccgggc gaggagattg acgttctgaa ggccgccggg
    13861 gctgagtacc aggaccggcc ggtggtcgtc gacggccgtc tgatcaccgc caacggtccg
    13921 gaggcggccg acgatttcgg ccgggccctg gtgcgggccc tggccgacca ggcggccggc
    13981 cgaaaatgaa gcggttcctg ggcgtggccg tccggtgcct ggtcagcggc ctgctgatct
    14041 tcatcctggc ggtgccggcc agccggttca tctcgccgcg cctgcccctg gtcgacatca
    14101 cggccctgcc ggcgacgttg gccgccgccg acccggccgc tctggccggt gcggtcctgc
    14161 tggcgctggc ctcggtgctt ttcatcgccg gccgctggtg gctgctggtc gggagccggt
    14221 cggtgcctta tcattttcta ttgcggctga ccttcatcgg catcttcttc aataacttcc
    14281 tgcctaccgg cgccggcggg gacctggtga agggatacta cctgctgcac ggccggagcg
    14341 gcaagttgga cctgggcctc tcgatcgtga tggaccgcct ggtgggcgtc ttctcgatca
    14401 tgaccctggg ttacctggcg gtgatcgtca atttcgaccg gctgcccggg gcggcggcct
    14461 ggacggtgac ggccatcttc ctggccctgc ttttctttct gctcctgctg gcctggcggg
    14521 gcctggccgt ccggctggcg cgccggctcg gcctggaggg ctggggccgt ttcggcgcgc
    14581 tgttgaagcg agtctatcaa ggcggccgtg cctacctgga ggaaccggcc cgcctgctgg
    14641 gatcgctggc ctgctccttc ggaagccagg gcctgcagct ggccgccttt tacctggtgg
    14701 cccgggcgct ggggacgccg gttacgttcg tttccctctg cgcctacgtg cccctggttt
    14761 ggtccttcgc gctggtgccc tcgctgggcg gcctgggggc gcgggagacc gcctacacgc
    14821 ttttttttca gggccagatg ggccgggaga acgcgctggc cctcggtctg atcatactcg
    14881 gcctgacgct gacccagagc ctggtcggcg ggctgcttta cttcctggga ggtggaagat
    14941 ggaaaacggc aagctgacct acgaacgaac cctggtctgg gataacctga gcgaggcgga
    15001 gaagaaggag gttttcgcct tcgccggggc ctaccgggcg tttctctccg aggtgaagac
    15061 cgaacgggaa ttcatcgacc gcaccgtctc gatggcccgg gcccgcggtt ttcgggaacc
    15121 gggcggccgc cgggcttcgg atcgggtctg cctggtcaac aagaacgcca cggtcggcct
    15181 gctggtccgg ggacgccgcc ccttcaagga cggcttccac ctggtggccg cccacatcga
    15241 cgcgccccgg ctcgacctca agccgcagcc gctggtcgag gacgaggggc tggccctgct
    15301 caagacccac tactacggcg gcatcaagaa gtaccagtgg gtgacccggt cgctcagcct
    15361 gcacggggtg gtggccctca agaacggcga gtgccggaag gtgacgctgg gcgaggcggc
    15421 cgatgaggcg gtcttcaccg tgaccgacct gctgccgcac ctggccaagg accagatgga
    15481 aaggaagatg tcggaggccg tccaggccga ggcgctcaac ctgctcatcg gctccctgcc
    15541 ggcggaagcg aagaaggccg atcagaagaa ggcccccggc cgggtcaagg aggcgatcct
    15601 gggccggctg cgggcgaagt acggcttcac cgaggaggac ttcatcagcg ccgacctcca
    15661 gatcgtaccg gccggtccgg cccgcgacct cggcctggac ggcagctgca tcctgggcta
    15721 cggccaggat gaccgcatct gcgcctacac cgccctcaag gccgtcctgg acctggacga
    15781 gcccgagtac acggccgtct gcctgctctt cgaccgcgag gagatcggca gcgagggagt
    15841 cggcagcgcc cagtcggtct tcctgcgcgt tttcctggcc gaggcctccg gcctgcaggg
    15901 gctggagctg gaccgcgtct gcggccgttc ctacgccctg agcgccgacg tgagcgccgg
    15961 cctcgatcct tcctacaagg atgtctccga gcctcagaat tcgccgcgcc tcggctatgg
    16021 cactgtcatg accaagtaca ccggctgggc cggcaagtac agcgccagcg aggccagcgc
    16081 cgagtacgtc gcctacctgc gccgcctctt cgagaagcac cgggtcttct ggcagaccgg
    16141 ggaactgggc aaggtcgaca agggcggcgg cggcaccgtg gccaagtacc tggcccgcca
    16201 gaacatcgag accatcgaca tcggcccggc cctgctctcc atgcactcgc cccaggaggt
    16261 cagccacaag gccgacctct ggtccagcta ccgttccttt ctcgcctttt accggggttg
    16321 accggcccgg ccggagatga ccatggctga cgggcgcaag cggctggccg aactggccga
    16381 ctggtaccgg gaccggccgc tggaccggga cctggaggcg cgggtgctct ccgaactggg
    16441 cctttccctg gcggcccgct acggcggcca gaccttgaag aacccgctgc tggtggcgcc
    16501 cggccagttg acgctgaccc tttcccaggt gcaggccatc cggaaggccg gttacgccgg
    16561 ctgcgtcctg aaatcggtgg tcggcgagag tccggacggc gcctgttcaa tggcgcgcca
    16621 gcggattaaa cccacctcgg tgcggaccta ctacgagccg gaggataccg ccggcgcctt
    16681 cccgatcatc cactgggacg gccggctgga catccgcggc ctggacgagt acctcgattt
    16741 cgccggggcc gcggcggccg aggacagcta cggctccttc agcgtcgtgg cctccctgct
    16801 ctgccacctg ccccggccgg gcgaggattt ccgggccgac gagtggagcc ataccgccgg
    16861 ccgcctcggc cagctgggct accggcacct cgagatcgat ttctgcccct tcctctccag
    16921 cgaccgctac accgaggacc aggccaacat cctgcgctgg taccggagcg tgccggccct
    16981 ggtcaagcgg gccgggcctt ccctgcgcgt cttcccgaaa ctgctcaacc tggattgggg
    17041 aatcgattac caggttcaaa tggcccgggc ggcggttgag ggcggcgccg acggactggt
    17101 ggtggccaac cgcggttacc ggccggaatt cgcctccggc cacggcgggc cgggcctgcg
    17161 ccgcctcaac ctcgagcagg tgcgccggat caaacgggag ttcccgggac tgccggtttc
    17221 ggccaccggc ggcgtctacc gcggtcgcga cgtgctggag tacctggagg ccggggccga
    17281 gaacgtccag ctgctcagtt acctgatggg ccgggtgaag gcgcccttcc ggcgcgggac
    17341 gggcaaccgc ctggagaagg ttctctacca attgatcttc gatccggacg atggcttgct
    17401 ggccgtctgg ctggacggtc cgaagcgagc cggagggatg acgaaatgaa ccgagtcgcc
    17461 tgcgtgaccg gcgccgccca gggaatcggc ctggccgtgg cccgggagct ggccggcctt
    17521 gatttccggg tgctggcggt cgatctgcgg ccggccggcg agggggccgg ggtgatcggc
    17581 ggcctgcccg gggcggacca ccgctacctg gcggccgaca tcagtgccgc ggccggccgg
    17641 gaagccatcc tgcgcctcct gcgggaggag ctgggccggc tcgacctgct ggtcaacaat
    17701 gccggcgtgg cgccccggga acggcgcgac ctgctggaga ccagcgagga gagtttcgac
    17761 cgggtgctga acatcaacct ccggggcacc ttcttcctca cccaggcggc cgcccgcttc
    17821 atggtggaac tggccgccgg cggggcgggg gccggcaaga tcgtcaacat ctcctcgatg
    17881 tccgcctaca cctcctcggt caaccggccc gagtactgcc tctccaaggc cggggtgagc
    17941 atgctcaccc gcctcttcgc cgaccggctg gccgagtacg gcatcaacgt ttacgagatc
    18001 cggcccggca tcatccgcac gccgatgacc gaggccgtgc aggcccgtta cgacgacctc
    18061 atcaacaacc ggggcctgct gcccatccgg cgctggggca ggccggagga tgtcgcggcc
    18121 gcggtccggg ccatcgccca ggacgccttt ccctattcga ccggccaggt cttcgacgtg
    18181 gacggcggct tccacctgcg ccggctttga atcggaccgg ccgccggtcc gcggcaagga
    18241 tgagagatga ccaagagcag cctgaacttt gagcccggcc gttcggcgcc gctttaccgc
    18301 tgccgggtcc tggtggtcgg cagcggcgcc gccggcctca actgcgccga gcagctccgc
    18361 ctggccgggg tcgacgacgt ggcggtcatc agcacccggc gcctgggcgg cacctcctac
    18421 cgttccggct ccgacaagca gacctactac aagctgagca acttcggcga caccctcgac
    18481 ggcccgatgg agatggccca cagcctcttc gacggcggcg cgatggacgg cggcctggcc
    18541 tacatcgaaa gcctctactc gctgccggcc ttctaccacc tggtcgagat cggcgtgcct
    18601 ttcccctcca accgctacgg cgccttcgtc ggctacaaga ccgaccacga cccgcgccag
    18661 cgggccacca gcgccggtcc caagacctcc cgttacatgg tggagaagga gcttctccgg
    18721 ctgaagtccc tgggcgtgcc ggtcctggac ggcctgaccg cggtccggct ggtgaccgcc
    18781 ggccgccggg tgctgggcct ggtcgccctg gacgaaaacc gctccgagga cgatccggcg
    18841 ctcggtctga ccgtcttcca ggccggcgcg gtggtgctgg ccaccggcgg ccccggcgaa
    18901 ctctacgcgg tttcggtcta tccggccggc caggtcagcc tgcacggggc ggcgctcgaa
    18961 gcgggtgcgg ccgcggtcaa cctgggcgaa tcccagttcg gcctggcctc gacccgtttc
    19021 cgctggaacc tctccggcac ctaccagcag gtgatccccg cctacttcag cctcggcccg
    19081 gacggccgcc ggcgcgactt cctcgccggc tggtaccgct ccctgccgga actggccggc
    19141 gccgtctttc tcaagggata ccagtggccc ttccacgtca accggctgga taatttcggc
    19201 tcctcgctgg ttgacctggc cgtcggcgcc gagaccgaat ccggccgccg ggtcttcctc
    19261 gatttcaccc gcaacccggc ccggcccggc gagtccttcg acctggactc cctgccgccg
    19321 gaggcgctca actacctcaa ccgttccggc gcccggcagg ccgatcccta ccggcgcctg
    19381 cgccacatga acccggagag catcgcgatt tacgccgagc acgggattga cctgcgcgag
    19441 ccgctggagg cggccgtctg cttccagcac aacaacggcg gcctgaaggt caacgccgac
    19501 tgggaaaccg acctggccgg cctctttgcc gccggcgagc tggccggcac ccacggggtg
    19561 acccggccgg gcggcgcggc gctcaacagc ggccaggtgg gcggcttccg gatcgcccgc
    19621 tgcctggccg gccggcggga cgagctggcc ggccgccggc cggcggcccc gttccaggcg
    19681 gccctgcgcg aggtttaccg gaccagccgg gcgatgctgg cctccgaccg ggagcagggg
    19741 cgctggcggc gcctgatcca ggaacggatg agcgccgcgg ccggtttccg acgcgagccg
    19801 gagcgggtgc gcgccgcccg ccgggaggcg gaggccgacc ggcgccgcct cgagtcggcc
    19861 ccgccgggcg ccgccggccg ccggaacctg gcccgcgcct gggagacccg caacctcgtc
    19921 ctgacccagc tggcttttct gaaaagcatt gacgcctacc tccagtccgg cggcggcagc
    19981 cgcggctccg ggctggtgct tgcagaggcg gccgtccccg gcgcccggcg ggttgaactg
    20041 gtcggcggcc gctccctctg cttccggccg gaacggcccg gcgaccggcg tctccgggtg
    20101 gcggtctccg gccccgcgtt tacgacctcc cttgaggagg tggcgccgct gccccgcgac
    20161 gaaagctggt tcgagaccgt ctgggccgac tggaaggccg gccggacttg cggcggcggc
    20221 aaacggcgcc gccgctgacc ggtaatggaa aagggtgaac tttcgatccg gaacggccgc
    20281 tgcctgctgg ccgacggccg cctggccgca accgacctgc gcctctccgg cggccggatc
    20341 gccgaaatcg gttccggcct gaagtccggc cgggaactgg acgccgccgg cggcctgctc
    20401 ctgcccgggc tggttgacct gcacacccac ggcctggaat tcgcgctggc cgagaccgcc
    20461 ggcctggacg agtacgcccg cctggaggcg gcccgcgggg tcaccgcctt ctgcccgacc
    20521 ttcttcaacg ccccggagaa cctgctcgaa accctgcgcc gccattgccg ggagaccggc
    20581 cgcctccagg cggtgcccca ggcggtcggc ttccgcctcg agtcgcccta tcttacccgc
    20641 accggtggcg gccggtccga cgacctggtt ccgatcacga ccggcctgac cgacgcgctg
    20701 ctggaggccg gtggcggcct gatcaaggtc tgggacattg cccccgagct ggagggcgcc
    20761 gtcgagctca tcggccgcct ctcgagccgg gggatcgtct gttcgctggg ccacaccaac
    20821 gccggcctcg agacggccgc ggccgcggtg gaggccggcg cccggctggt gacccacctc
    20881 ttcaacgtca tgccgccgcc ggacccggcg gagccgggcc tcacgccggt cggcctggcc
    20941 gattacttcc tggtcgagcc gcgcctctgg gccgagatca tccccgacgg gacccacgtg
    21001 gcgccgatcc tggtccggga ggccctgcgc tgcaagggtc cggaccgact gatcttcatc
    21061 accgacagca actgcggggc cggcctggcg ccgggcgact acctgtttcc caaccgctgg
    21121 gggcgggtgc ggattggcgg cagcgaggcc ggcgtgcggc tgatcgaccg ggacctggtc
    21181 ctggccggca gcgccctgac ggccctggac gccttccggg ccgcggtcaa ccgtttcggc
    21241 caggacctgg cggccgccag ccggctctgc tcaaccaacc cggcccgcct gctgggtctg
    21301 aacaagggcg agatcgcccc cggccgggat gccgacctgg tcattctcga cgaccggctg
    21361 gatttgattt gcaccgtcgc ggccggccgg gtggtttacg agcgcgcccc ggccgccggc
    21421 attgcgtaag aggagggaaa ccggatgtcg aaattccaat acgagccggc ccggtgcgtt
    21481 ccctttcgcg accgggccgt cctggagcgc tgccgccgca tccgggccgc cgcgattacc
    21541 cgtcacccca acccggactt ccggatccgg gtcgtcccgg acgccaagat cacccgcctc
    21601 tggctggtcg acatgttcga gcgcatccgg accgccgccg aggcgggccg gccgctgatc
    21661 ctgatcacgc cccagccctg gccggacggc taccgggaac tggccggcct gatcaatcgc
    21721 caccgggtca attgtaaaaa actgcacacc ttcaacatgg acgagtacgc caacgaaaaa
    21781 ggagagacgg ccccggagac ctggccggcc ggcttcctgt actcgatgct taataatttc
    21841 taccgccggc tcgacccgaa actccggccg ccgcgccgcc aggtccaggg gccgacccac
    21901 cgcaacatcc gggattacgg gaagatgatc gccgacctgg gcggcgccga ctgctgctat
    21961 agcggcccgg gctggaccgg ccacctggcc ttcatcgagc ccgacgcgcc ggagttccgg
    22021 ggcggcctgg cggcctggaa aaagatgggg ccgcgcctgg tgaccctgag tcccttcacc
    22081 atcgcccaga actccctgca cgcggccttc ggaatgagcg gcgacctggc cgcggtgccg
    22141 ccccaggcgg cgaccatcgg cccggccgag gtcatcgccg cccgcttccg tttcgacatg
    22201 cacgccatca ccgtcggcgg cagccgggtc tcctggcagc ggctgatgac ccgtctctgc
    22261 ctgcacggac cggtgacgcc gaagcttccc cagtccatcc tgcagaccct gcgcaccgac
    22321 gtttacgttt ccgagagcat cgccgccgac atcgagcccg attacgacgg cggttattga
    22381 agccgacaat caagggaggt ccggccatga aagagacgct ggaacggatt caaaaggggc
    22441 gggtgatcgc cgtcctgcgc ggcgacaccg tcgagaagac ggtgcggacg gccgaggccc
    22501 tggtcgaggg cggcgtgaac ctgatcgaga tcacctacac catggacgac ccgctggcgg
    22561 ccatcgagcg gctggccggc cgcgatgaca tcctgctggg cgccggcacc gtcctgagcc
    22621 tgaaggacgc ccgggcggcc cgggcggccg gcgcgcgttt cctggtcagc ccctgcctga
    22681 ttcccgaggt ggtcggctac ggccgggagg agaacctgct ggtgatgccg ggcatcttca
    22741 caccgaccga ggccttccag gccctctccc tgggcgccga ggttttgaaa ctcttccccg
    22801 gttccctggg cggcccggac ctgatcaagg ccttccgggg tccctttccg ggcttgaagc
    22861 tggtgccgac cggcggcgtc gagaaggaga acctccgggc ctggttcgcc gccggcgccc
    22921 tggcggtggg catgggcggc aacctggcgc ccagagaggc catccagcgg gaggattacg
    22981 cttcgattcg ggagaaggcc cgcgagatca tgggtctgct tccatgagcg gggaggagag
    23041 cctggagatc aagggcgtcc gggtgaccgt tgtccagggc gacctcaccg cccaggaggt
    23101 gaccgccatc gtcaacgccg ccaacaacaa attcctgatg ggcggcggcg tggccggagc
    23161 catccgcaag cggggcggag ccgaaatcga ggccgaggcg gtccgtctcg gaccgaaacc
    23221 ggtgggcgag gcggtggtca cctcggccgg ccgcctgccc tgccggcacg tgatccacgc
    23281 ggccaccatg gccatggact ttcagaccga cgagacgaag atccgaatgg cgacccgcaa
    23341 cgccttgaaa cgggtgcggg aaaacgggat ggagatcctc gccttcccgg cgctcggctg
    23401 cggggtgggc ggttttccgg tcgaccgggc cgcccggatc atggccgagg agacggccgc
    23461 ctttctggtg gaaggttc
//
LOCUS       DAOQYN010000005        23443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_2528_length_23443_cov_4.986703, whole genome shotgun sequence.
ACCESSION   DAOQYN010000005 DAOQYN010000000
VERSION     DAOQYN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 23443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23443
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2528_length_23443_cov_4.986703"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PKN80_00470"
     CDS             complement(<1..223)
                     /locus_tag="PKN80_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34526.1"
                     /translation="MLAYGRRGHALTRRRVAAMADLRGFDFVGFADDDATSYAIEAID
                     DDLGLLIEVSGAVLFQGQAFEAAGCAVTDF"
     gene            197..3253
                     /locus_tag="PKN80_00475"
     CDS             197..3253
                     /locus_tag="PKN80_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34527.1"
                     /translation="MAAPAVGEHVGIGQPHGNIGYDNPIDYSDMNRLAVQYITAMKKV
                     NQYWKREINWYGCDSFLEAADRMGFFVTLRMSGTGFQAETFWAGNPSSQQKALATARW
                     LRNHPSVASFIVRQSGNYAQTDTTPYGETAFWWENFKEDASWDAYHRVWEEFVAPLRE
                     TSPSILFSGMWLPYGQDLGPSGTKFGYGVSLQSREEMPRFWSGRGLARYHAEEGWPQI
                     SRYSWDATGQDDLSKYYELYWPNMSQVTEELAAMYFGDLPYDQAASSGNGFGSLWKKD
                     SDGQGRFSWEYVSKNQVVGLPYSRKFTGPFETPGYVKLRSLFPHNTIRSWRWYGYGYW
                     EHTESGVYFEDSWNFLNKEPEKFTRTLDNIRRRPGLFIDKARSDTRLVADYATLAGAS
                     IPLSEVGAVAEKVTRPLAAFVLGWPDSVRKDHAFYAGELIRKQVGILNEYPGPLAGEV
                     RWNFQERENGRPVYQGVIPLDFRNGDNQCVGIEFRAPAVKAKTRYRLCLDTLRREASG
                     RLASVHDDAFDIEVFPPRSAPAISGKIGLIDTGAAGSLFQIAGIPFAPVTEKSNLAEY
                     DLLVIGRGRTSEATRALLKKLELPKHLAAGLNLLVLEQEGEVLERTGQEDWPRRAWKE
                     RGDRPELLSFGLRQERQSFRNVFIRVPGHRLFVGLSDEDFANWRGASNLIPPYTKEYW
                     YRAESGRSRRNANASNFGDVVTYALEKPQGGNFVSLVDAGFDLLQSALLEEIRPHGRT
                     LYCQLNVSNRYGVDPAATLLVDRLVAYALEKPAPLVGRAVRLAGAGWDRVLAGLPFDL
                     AAAGSNLSASDLKNYNLLVAGIGRPRIEVFDDKNKFAGQEAVPGRPGQDPVLDWLGRN
                     RREVADFVEKGGAVLVLPVQSAAELEWLPFELKLEPVKFFAAAPGDFFASGAIGPADL
                     YYRRALELPVPAGLPAGSRSTRPAILARVPYGRGEFIFAQLHPKSISGNWAESKLARV
                     LSTILTERGVADRLDLDPLGRGYGPGGYPYQVGTPVYFDPYLSTDW"
     gene            3258..4547
                     /locus_tag="PKN80_00480"
     CDS             3258..4547
                     /locus_tag="PKN80_00480"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNQ34528.1"
                     /translation="MKTTDQVRDYIRESREIGLGLLKPSACDLEHGLELHAESVICDA
                     YGFSPRAALDSEALNALVEAGASAVEFREAQEEMIMTRCLTDAGQRAEFQAAWEAAGV
                     TCLFQNAGTEDRPPLAMLKRLAHYTLVTDLLDGFIEKAVTPGAIPAAKAAGRRCLYLT
                     CNGLPLPGRLVSVAEELSLLKIFFELGCRMMHLTYNRRNLLGDGCGESADAGLSDFGR
                     QAIAEMNRAGLIVDVAHSGQRTSLEAARASARPAVASHSVCAGLNDHIRGKGDDVVRA
                     LADSGGYIGICGVPQFLGGRGDITAFLDHIDYAVGRFGDDHVAIGTDRAYHSVLSQSE
                     RARLGPVPAGSNNWQSLWPAGSLPYRPDWQKPEQLRSLEWTNWPLFTVGLVQRGHSDE
                     RIRKIIGLNVLRVARANFPYDRYPGLAVPETGAAPAD"
     gene            4560..6626
                     /locus_tag="PKN80_00485"
     CDS             4560..6626
                     /locus_tag="PKN80_00485"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNQ34529.1"
                     /translation="MPDLKVLAAAVDRYYSDLGPFRPEPPRPADTVNWCRALRQGQVL
                     FYGCHPVKVGLKEIDWSGRQLDQFEWPHQLNRFFWLTHLARSYREGGGEEPARLARLT
                     IEDWIDQHDYRAASEPLPGDGWFNMSIRLGQSHFPGWWGNLPAFAGSPHFPADFVDRM
                     LESTRGQAAGLAGHLTPLGNHRISELDSLLFLGLLLPGFEAYARLAVEKLNQDFYRQV
                     EPDGSHLEHTPQYHDWMCVAFTRFWMLGRARPELGLKLDTGRVGLMWDYAVHAAAPDG
                     GSFGLHDSFVWDKPSGEGPAAWQPAATPPPASRVMEQRTAFLAAAGLAGKPGWDLDRR
                     PVRYFPAAGQCFFRTGWDRRATGLVFDATRWGRGHMHLSRLGLSLYAGDRMLLYDPGI
                     FSYHMKDPFASYGRSTISHNTVNLAGFNQSDADCEVRRCCLGRGAAVFSAAYEGGYYP
                     GKYTWFFDDGKGSGLYGAHLRTVIWVDGRFILVCDHLRSDGRGQPYASHWQFPAGPVR
                     LDPAGRRAWTLGEDNLMLQVLDSMDELELSLHEGEREPLLGWLPDQRLEAHPAPLLAV
                     SGRAGHPAVFWSTLLLPFRGGSPPDFRLERFKSYGECGFGFRFDWPDGRRDIVALTPR
                     LERAINLVEPLGPLDTDASVAVLSFRPDRKSWRAFLMDGTRLNFEGKPVLEKEKAGVY
                     TAVFRG"
     gene            6675..9236
                     /locus_tag="PKN80_00490"
     CDS             6675..9236
                     /locus_tag="PKN80_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNQ34530.1"
                     /translation="MEGRRFSPGRARIPAGLVLLLLAAACLPAGLSGQTAGASGAAVT
                     RGTYRGPVFDGLATVEISNPVLKVVTVPERGGRVAQVIHLKSGRDFVNLSAATALEKE
                     NGIYERLDPSGRQPGRAAPDNFMGMPFKAEAAGPGSVRYTCQAGSFRVERVLTLDPAL
                     PRLDVRTTYTNVDTKPVTTQFFPIYQLNTNQDRTTLYDVLVFPDSRAPGGFKRYPEPT
                     REFHFETQFGWFFISDLRSREAVVVTHRPNDPIIDSTYSYKVTTFQHLVVLGREQELA
                     PQASMTLEVGLWFISGAGEVPALAGAAGLIGPENGRRLQQALAAELAQPAPGAAAFVL
                     PKVDEKAIYRPPVEKPRLTLARIFWDGVYANPEREDFLQLTSSQYLLEAVAEVNAIKP
                     DLLVIQGGAYNGLEKEYADLKKITGLARVPVSVTPGRRDKPENWLKSFDWNYQHLQVE
                     GQHLFLLPAWSFLKADGRKALIQELEKELAALAGTDRAVILCPDPPRLRQNSGKDVPA
                     VPELLDLVNRYPVVLYLSGSGTNLLQRDGKATVVGSYELNNSRSYQLIRFYPDRIEVD
                     LAKPLGDNFSPVVTVPYAPDLKRTALYPGLPAGEKRRPDYFFVHLSDSQFGDSAAQQN
                     PAGRNFNDFGRFLQLTSETNRFKPAFLINSGDLVERGTNEEQWRIYLKATATSRRPVH
                     TAIGNHDAGPEHYTRWTKEQPLRLQKQGKDAFIYLMNDTSSASRGEGLSPELMARLRV
                     LLEKSRSARQLFLVNHFPLDAMPGQAAELKALLEEYRPTLFMAGHTHNLGYEWRGETF
                     QAVANSIGWTKQGPNTSYTGYFINYVFPEQVVVVYKPLYEDVGWSVTVPNPRRKK"
     gene            9287..10288
                     /locus_tag="PKN80_00495"
     CDS             9287..10288
                     /locus_tag="PKN80_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34531.1"
                     /translation="MGGGRKLNWGIIGCGALAERQLAPAIQAGANSRLAAVYSRDLAR
                     GEDFARRHGAGRAYDDLAAFLADPELEVVYIGTPPHLHCEQAVAASRAGKHILCDKPM
                     AMNSAEAARMVEAARAAGVKLMAGFMMRFNVYNRKAAELLRAGALGKLIHIRIQASYN
                     LPDTSFNWRQDPTLAGGGSLFDMGSHAVDLLRFFNGEIASALAFVDNRAYAYPVEDTA
                     LALLRFAGGGYATLDASFSEKAPSRNIDIFGNQGSLWLTGSIGRNATGVLNGFLNGER
                     KEFTLEPKHIYISEVEELGRAILEDRPPEVSGEDGWRNLLVLEAIYRSGREGRLVNL"
     gene            10406..11314
                     /locus_tag="PKN80_00500"
     CDS             10406..11314
                     /locus_tag="PKN80_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ34532.1"
                     /translation="MMRKWPIAFGCLLLAAFLFRAGPVLAQAGPGHLFRFAAMGDPQP
                     GLDNPGNYGTHLNQFKRAIRQVNESGAEFTIILGDLVNNGQVDTEYENIAAAAKSFGK
                     PWYPVRGNHDAPEKFKQYFKSELNYSFEHKDFRFVMLDAVGGQEELTVEQLAWVEKEF
                     QAAKTKKQEIVVCTHVSAWDKNPRGASPYMQIGPESKAKLKELYKKYDALLMLSGHYH
                     RGPWYAQEEGMNYLVLPGPAMPRGSPSSWQIFDVYPDRVESYTKQIYLPYDDETAAGY
                     INTPYLTWVAYGPEQARVFVMKRNVK"
     gene            11370..12959
                     /locus_tag="PKN80_00505"
     CDS             11370..12959
                     /locus_tag="PKN80_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ34533.1"
                     /translation="MNDRRRFWMRLGLAVMLAGLLAGPAFAQAGNVLWQEDFEGYAEN
                     APLDGQNGWTMLKVADAPAAVVKSGAGLKNSRGLVLSNTKAFRSDYIGLTRTFPQALT
                     GTLWIQCRFKAPAAADNWIGGFNIKSNLAGFSAGLLREKDKPDQIRFVVPYWQGYRIH
                     RTVPYEGGRWYTLTLKLDNERKTYSAWVDDVELGSDLDVGAGELKSVYLSSGGKAEDP
                     AVVDDLIITRSRPAGITERPLYPAGKPGRLFRFAAMGDPQPGLGNYAVELIHFQRAVR
                     QVNESGAEFTLILGDLVHDGKVDTTYQDIVETARGFGKPWYPVRGNHDAPEKFKKYFK
                     PELNYSFEHKDFRFVMLDAIGGQEELTAEQLAWVEKEFQAAKAKKQEIVVCTHVSAWD
                     ENARGVSPYMQIGPESKAKLKELYKKYDALLMLSGHYHRGPWYAQEEGMNYLVLGGTG
                     FVRSSPTGWSVVDVYPDRLEIYTKPVFFPCEDEKTTEFYDMPYLEWRSYESYRTGQGV
                     AAKLHFPYLVRSPLVIKRKAR"
     gene            13012..14559
                     /locus_tag="PKN80_00510"
     CDS             13012..14559
                     /locus_tag="PKN80_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ34534.1"
                     /translation="MLKINRGCCLAFAAIFLAGLLAGPAFAQTKGIIWQEDFEGYAGE
                     AVFPAPAGGEVKNGLVKAGVGFKGGRALAVDSLKVNVPLAAGLAEPVWVQCRVKAAAG
                     SRQLNLNVSTNLFVVCWAKPVEAQKEQPAYTQLVVPYYDGYRIYTPLKLDDAEWTTLT
                     FKLDPVRHAQTTWVNDTVMVGECDLPDQPLLSVSLAFGGVPDKPVLVDDLSVRTVKPA
                     DLRDRPLYPAGKPGRLFRFAAMGDPQPGLENYNVELHQFRRAVKQVNESGAEFTLILG
                     DLVHDGKVDTTYQDLAATAKSFSKPWYPVRGNHDAPEKFKQYFKSELNYSFEHKDFRF
                     VMLDAVGGQEELTAEQLAWVEKEFQAAKTKKQEIVVCTHVSVWDDNLRGVSPYMQIGP
                     ESKAKLKELYKKYNALLMLSGHYHRGPWLHQEEKMSYLVLPGPAWPRNSPSSWQIFDV
                     YPDRVEMYTKQVFLPYDDETATGFINIPYQSWVNYETLRTGKDVPAKLHFPYLVQGPL
                     VIKRNVR"
     gene            14639..15607
                     /locus_tag="PKN80_00515"
     CDS             14639..15607
                     /locus_tag="PKN80_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34535.1"
                     /translation="MKKSEICFVYFNDAGMWRPSDLAGHFEDMKSVGGESIFTNFLEG
                     SPDGLAFRTRLAHAAGLKVYASPGRIAGLFAAGPRPSSMFPVRRPDTLMRDIKGQPVL
                     ATSGLVCCVNNPGFQEWFFPHIAEILQKTGVDGVVFDEPKEAHMACYCEYCRALAGDD
                     AAALVALHEKALAETMGRVAGEFKAFRPGGAGLVMLMPNATENFIKEVCRQPAIDCVG
                     VDGPLCLQNSRPVTPPHQKTYILESAPPFFKLIRAAGKQTFALAETFGVYAWALDELR
                     RNIGKFAELGADVAAFNYYGHDSDDPEAVMEVVREAARLVRDRGTE"
     gene            15659..17245
                     /locus_tag="PKN80_00520"
     CDS             15659..17245
                     /locus_tag="PKN80_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ34536.1"
                     /translation="MPKKKRFWQLAGLLILLLAAAVGPAFAQAGNVLWQEDFEGYADN
                     SALNGQNGWTMLKTADTPAGVVKAAAGTGNSKGLALSNSKGFRSDYIGLTRNFAEPLT
                     GTLWIQCRFRMPAAENWTAGFNLKTNLAQVSAGLVQEKDKPARLRFVFPYYEGNRIWR
                     EIPFEAGRWYTVTLKLDTAGRTYSGWVDGIELGGYIEMAGSELKSVYLGAGGQEGAPA
                     VVDDLVVTRNRPAGINEKPAYPAREAGHLFRFAAIGDPQPGKWRINNYQHDLYKLGRV
                     AAQVNESGADFSIVTGDLVHDAVSDTPFLDALEKLKVLKRPWYPVRGEQELVDLYRKH
                     IRPELNYSFEHQGFRFVMLDAVGGETPLAAGQLDWVEKEFQAAAAKKQEIILCSHVSP
                     WDENRLATAPADRLAPESKARLVELLKRYRVLMTLAGHYHRGLWHFQADGVNYLVLPV
                     TSMARVSPAAWTVFDVYPDRVVVTVKPLFFAAEDAETKEFYDFPYQTWRSYQALRAGA
                     EPKSHYPYQVGGPLVIKRTR"
     gene            17260..18705
                     /locus_tag="PKN80_00525"
     CDS             17260..18705
                     /locus_tag="PKN80_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34537.1"
                     /translation="MNGLDWKVGLPFLFFLAASSLPAAADNGPVRDIGDRLELFVDDW
                     LIERLAGAELRLNEPALREVVFRFDAPGEGSTCLPSVILKDGDRYRMYYRGEPVKGLE
                     NTRYAESADGITWSRVKAGLYEENGSRENNIVFREPETQELEVFIDGNPAAPTGERYK
                     AFCRWKGKDGEGRAVRGIKILASADGIRWRLLQSDPVISLLRPKETSILDSDNRAFWD
                     AARNRYAGYLRVFRDGFRSIKFFASKDFRTWGEPEWLDFGPAPAEHLYTNVIGPYFRA
                     PHILLGFPKRFLPDRKFLEEYRWKGICDTCFISSRDGRKWDRRFMEAFLRPGVDSRNW
                     VYHSPTLAAGGPVETAPGELSFYILEHYGLPDVRLRRATIRTDGFVSVRAGFAGGELL
                     TRPLTFSGRELVLNYATSAAGRVRVEIQDPEGKPRPGFTLEDCPEIYGDQLERPVAWK
                     GGSDLSALAGRPVRLRFQLKDADLYSIRFRR"
     gene            18747..19685
                     /locus_tag="PKN80_00530"
     CDS             18747..19685
                     /locus_tag="PKN80_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34538.1"
                     /translation="MKKIGCVNIDTSHPRSFGEYLKKSGRAGYYAIYNEGFRGDDEVA
                     GFMKTYGVEKRCATLEELAGSVDACFIHSCDWDRNLRLAEPLLKLGKPVFIDKPIAGR
                     LSDCRKLEALAAAGAVILGCSSVRYAPEVAELAGKPEAERGRFLTVFGSVGVDEFNYA
                     IHIVEAIGGLLGTGAVSCRFLGRVEAEGKKSETFLVRFGSGVTAFYNTCLGVGQPFEM
                     TAVTTRTTHHFRVDASRIYGALLDRICDYLETGVNHLAPVAALTESVKIMLAGRLSRE
                     RGGAEVGLEEIPADDPGFDGTAFGAGYAAASRKIYA"
     gene            19685..20680
                     /locus_tag="PKN80_00535"
     CDS             19685..20680
                     /locus_tag="PKN80_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNQ34539.1"
                     /translation="MKPPLRAVCAEFGLGAPRGAIRLAGTRNLNYRLRTERGEWLLRR
                     RYAGYNQPARLDFDAAAARFLAGAGLPVAPPLPSRSGAAAWSRKGEQWQVYPFLPGRP
                     LVDGDPADAAALGRALAVWHRLGRDFSPDFEKLGTWGELAPSELRRVLERLGGETPGL
                     EDRLDDYRGLMETTARQLDSKAGWPLTLVHGDLQPGNILVEADRVSGFLDLDWCGRRS
                     RLYDLAFVFLFCCAGHDRPLAGGDIWTLTQPPRWRRPAFESCAAAYFSDAGSRLTAAE
                     AAELPARVRLAWCHTRLHGALKVARGRRRYFLGREGWSDPPADIYNFFTRGEWPR"
     gene            20677..22623
                     /locus_tag="PKN80_00540"
     CDS             20677..22623
                     /locus_tag="PKN80_00540"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HNQ34540.1"
                     /translation="MKRTPFPIPNNHADYELDPSPDRIGVKFDRPPAAGRYVTFKVTV
                     VLAEPLEAGQGLAVFSPFGFTPPQLRTPSAAGFVTVSGPAGAELKLTHYLVGKVKRER
                     GVIAWPVTTGLKRGSRLVFTFGDRSRGGPGSRVSLVAHQGLLVAGYRLRSQEEAAPPL
                     LARSPRIAVRPDRGALVRGFATPVLAAGEEGRLQLVAEDRFGNRADDFRGEVRLRGVE
                     NLAGLPDKVVFKPADRGRRELSFRPRRTGCFTPVVVSARADHLAGPVEVTERPPEYRL
                     YFGELHAHTELSQDGAGSLDEFYAFARSAAGLDFAAATDHQLPVAGMPGYASHASGLP
                     NTSHARYPDRWRATAEAARRHHQPGRFVTFLGFEFNTSGNAGHRNLYFKGDEPEIIEA
                     PAWPLPERFLQSWAGQRRDIMVVPHHPAICFSAGVYTTYKGLEIGVVDERIQPLVEIY
                     SKHGTSEYFNNPRPLRGQAVGHFVQDMLEAGAHFGLVGGSDGHQANPGSSLEEPGWFR
                     TLQYRSGLAAVWARELTREALWEAFFARRVYATTYPRVILRFQVNDLFMGAIGPAAYP
                     RRIKIFVASPILVNYLEVIKNGRVVFMTPEQGQNHPIPPEVEGELEFVDETPSDRVED
                     YYYLRLTLHGSERVWSSPVWVSNQ"
     gene            22714..>23443
                     /locus_tag="PKN80_00545"
     CDS             22714..>23443
                     /locus_tag="PKN80_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34541.1"
                     /translation="MKKSVIGIICLFIALSSSAFGQTVPAERNILWDGGFELGYGNNF
                     WGTVYGNQGPVLRSMWSGGVLKLNQRVASRVYILEDGTYTLCAWVKRAPGFEAQESRV
                     SLLLTNFNYYRETQKNLYGKTFPVAAGAGWQRVGWSFEVQGPVRNDFHVEISTGNPAG
                     SVLVDGMSLTRGSVLPDKLPAAAAVEAGFWIPEASGIYLDGEERAAELMIRNHGAARA
                     ARVRWEIYDHQEELVKRGEVNETLP"
BASE COUNT         3948 a         7653 c         7849 g         3993 t
ORIGIN      
        1 cgaagtcggt caccgcacag cccgcggctt cgaacgcttg gccttggaag agcaccgcgc
       61 cgctgacctc gatgagcagc ccgaggtcgt cgtcgatggc ctcgatcgcg tagctcgtgg
      121 cgtcgtcgtc cgcgaagccg acgaagtcga agcctcgcag atcggccatt gcggcgacgc
      181 ggcgccgggt cagggcatgg ccgcgccggc cgtaggcgag catgtcggca tcgggcagcc
      241 gcacgggaac atcggttacg acaatccgat cgattactcc gacatgaacc ggctggccgt
      301 ccagtacatc acggccatga aaaaggtcaa ccagtactgg aagcgggaga tcaactggta
      361 cggctgcgac agcttcctgg aggcggccga ccggatgggc ttcttcgtca ccctccggat
      421 gtccggcacc ggcttccagg ccgagacctt ctgggccggc aatccctcgt ctcagcagaa
      481 ggcgctggcc acggcccgct ggctgcgcaa ccacccgtcg gtggcctcct tcatagtgcg
      541 ccagtcgggc aattacgccc agacggacac gactccctac ggcgagaccg ccttctggtg
      601 ggagaacttc aaggaggatg cctcctggga tgcctaccac cgggtctggg aggagttcgt
      661 ggccccgctc cgcgaaacca gcccgagcat cctcttttcc ggcatgtggc ttccctacgg
      721 ccaggacctg gggccgagcg gaaccaagtt cggttacggc gtctccctcc agtcgcgcga
      781 ggagatgccg cgcttctggt ccggccgggg cctggcccgc taccacgccg aggagggctg
      841 gccccagatt tcgcgctaca gctgggatgc caccggccag gacgacctgt ccaagtacta
      901 cgagctttac tggcccaaca tgagccaggt taccgaagaa ctggccgcca tgtacttcgg
      961 ggaccttccc tacgaccagg cggcgtcgag cgggaacggc ttcggctccc tctggaagaa
     1021 ggattccgac ggccagggcc gcttcagctg ggagtacgtc agcaagaacc aggtggtcgg
     1081 ccttccctat tcgcgcaagt tcaccgggcc gttcgagacg ccgggttacg tgaagctgcg
     1141 ctccctcttt ccccacaaca ccatccgttc ctggcgctgg tacggctacg ggtactggga
     1201 gcataccgag tccggggtct attttgagga cagctggaac tttttgaaca aggagcccga
     1261 aaaattcacc cggacgctgg ataatatccg gcggcggccc ggcctcttca tcgataaggc
     1321 ccggagcgat acccggctgg tggccgatta cgccaccctg gccggggctt cgatacctct
     1381 ttcggaggtc ggcgcggtgg ccgagaaggt cacccgtccg ctggccgcct tcgtgctcgg
     1441 ctggccggac agcgtccgca aggaccacgc cttctacgcc ggcgaactga tccgcaagca
     1501 ggtcgggatt ttgaacgagt atcccgggcc gctggcgggc gaggtgcgct ggaacttcca
     1561 ggaacgggag aacggccggc cggtttacca gggtgtcatc ccgctcgact tccggaacgg
     1621 ggacaaccag tgcgtcggaa tcgaattccg ggcgccggcg gtgaaggcca aaacccgcta
     1681 ccggctctgc ctcgacactc ttcggcggga ggcgtccggc cggctggcga gcgtccacga
     1741 cgacgccttt gacatcgagg tctttccgcc ccggagcgcg ccggccatca gcggcaagat
     1801 cggtctcatt gataccggtg ccgccggcag cctgttccag atcgccggca tccccttcgc
     1861 gccggtcacc gagaagtcga acctggccga gtacgacctg ctggtcatcg gccgcggccg
     1921 gacctcggag gcgacccggg cgctgctcaa aaaactggag ctgccgaagc acctggccgc
     1981 cggcctgaac ctgctggttc tggaacagga gggcgaggtg ctggaacgga ccggccagga
     2041 ggactggccc cggcgcgcct ggaaggagcg cggcgaccgg ccggaactgc tcagtttcgg
     2101 tttgcgccag gagcgccagt ccttccggaa cgtcttcatc cgggttcccg gccaccggct
     2161 cttcgtcggc ctctccgacg aggatttcgc caactggcgc ggggccagca acctgatacc
     2221 gccttacacc aaggaatact ggtaccgggc cgaatccggc cgcagccggc gcaatgccaa
     2281 cgcttccaac ttcggggacg tggttaccta cgcgctggag aagccgcagg gcgggaattt
     2341 cgtctccctg gtggacgccg ggttcgacct gcttcagtcg gccctgctgg aggagatccg
     2401 gccccacggc cggacgcttt actgccagtt gaacgtcagc aaccgctacg gggttgaccc
     2461 ggccgccacc ctgctggttg accggctggt cgcctatgcc ctcgagaaac cggcgcccct
     2521 ggtcggccgg gcggtccggc tggccggcgc cggctgggat cgggtcctgg ccggtctacc
     2581 cttcgacctg gccgcggccg gttcgaacct gtccgcctcg gatctgaaga attacaatct
     2641 gctggtggcg ggcatcggcc gcccccggat cgaggtcttc gacgataaga acaagttcgc
     2701 cggccaggag gcggtcccgg gccgccccgg ccaggacccg gtcctggact ggctcggccg
     2761 caaccggcgc gaagtggccg atttcgtgga gaagggcggc gcggtgctgg tgcttccggt
     2821 ccagtcggcg gccgaactgg agtggctgcc cttcgaactg aagctggaac cggtcaagtt
     2881 ctttgccgcc gcgccgggcg atttcttcgc cagcggcgcc atcggcccgg ccgacctcta
     2941 ctaccggcgc gccctggaac tgccggtccc ggccggcctg cccgccggca gccggagcac
     3001 ccgcccggcc atcctggccc gggttcccta cggccggggc gagttcatct tcgcccagct
     3061 gcacccgaaa tcgatcagcg gcaactgggc cgagtcgaaa ctggcccgcg tcctctcgac
     3121 catcctgacc gagcggggcg tggccgaccg gctggacctc gacccgctcg gccgcggtta
     3181 cggaccgggc ggctatccct accaggtcgg gacgccggtc tactttgatc cctacctgag
     3241 caccgactgg tgagcggatg aagacaacgg accaggtaag ggattatatc cgggagagcc
     3301 gcgaaatagg cctggggttg ctcaagccct cggcttgcga tctggagcac gggctggaac
     3361 tgcacgccga atccgtgatc tgcgacgcct acggtttctc gccgcgggcg gccctggatt
     3421 cggaggcgct caacgccctg gtcgaggcgg gcgcctcggc ggtcgagttc cgggaggccc
     3481 aggaggagat gattatgacc cgctgcctga ccgatgccgg gcagcgggcc gagttccagg
     3541 ccgcctggga ggccgccggg gtcacctgcc tgtttcagaa tgccggcacc gaggaccggc
     3601 cgccgctggc gatgctcaag cgcctggccc actataccct ggtcaccgac ctgctggacg
     3661 ggttcatcga gaaggcggtc acgcccgggg ctatcccggc ggccaaggcg gccggccgcc
     3721 gctgcctcta cctgacctgc aacggcctgc ccctgccggg ccggctggtc tcggtggccg
     3781 aggagttgag cctgcttaaa atcttcttcg aactcggctg ccggatgatg cacctgacct
     3841 acaaccggcg caacctgctg ggcgacggct gcggggagag cgccgacgcc ggcctgagcg
     3901 atttcggccg ccaggccatc gccgagatga accgggccgg cctgatcgtc gacgtcgccc
     3961 actccggcca gcggaccagc ctggaggcgg cccgggcttc ggcccggccg gcggtggcca
     4021 gccactcggt ctgcgccggc ctgaacgacc atatccgggg caagggcgac gatgtcgtcc
     4081 gggctctggc cgacagcggc ggatacatcg gcatctgcgg cgtgccgcag ttcctgggcg
     4141 gccgcggcga catcaccgcc tttctcgacc acatcgacta cgcggtcggc cggttcggtg
     4201 acgatcacgt ggccatcggg acggaccggg cctaccactc ggtcctgagc cagtccgagc
     4261 gggcccggct ggggccggtt cccgccgggt ccaacaactg gcagtcgctc tggccggccg
     4321 gcagccttcc ctaccggccc gactggcaga agccggagca gctccggagc ctggagtgga
     4381 ccaactggcc ccttttcacg gtgggcctgg tccagcgcgg ccactccgac gagcggatcc
     4441 ggaagatcat cggcctcaat gtcctgcggg tggcccgggc caacttcccc tatgaccgct
     4501 atcccggcct ggccgttccg gagaccggcg ccgcgccggc cgattgaaga aggagaggaa
     4561 tgcccgatct caaggttctg gccgccgccg tcgaccgcta ctactcggac ctgggtccgt
     4621 tccggcccga gccgccccgt ccggccgata ccgttaactg gtgccgggcc ctgcggcagg
     4681 ggcaggtgct tttttacggc tgccatccgg tcaaggtcgg cctgaaggag atcgactgga
     4741 gcggccggca gctggaccag ttcgagtggc cgcaccagct gaaccgcttc ttctggctga
     4801 cccacctggc ccgctcctac cgggagggcg gcggcgagga gccggcccgg ctggcccggc
     4861 tgacgatcga ggactggatt gaccagcacg attaccgggc ggcgtcggaa ccgctgccgg
     4921 gcgacggctg gttcaacatg tccatccggc tggggcagag ccactttccc ggctggtggg
     4981 gaaacctgcc cgcctttgcc ggcagccccc atttcccggc cgatttcgtg gacaggatgc
     5041 tggagtccac ccgcggccag gccgccggcc tggccggcca tctcaccccg ctgggaaacc
     5101 accgcatcag cgaactggac tcccttcttt tcctgggcct gctgctgccc ggctttgaag
     5161 cttacgcccg gctggccgtc gagaagctca accaggattt ttaccgccag gtcgaaccgg
     5221 acggttccca cctggagcac acgccccagt atcacgactg gatgtgcgtg gccttcaccc
     5281 gtttctggat gctgggccgg gcccggccgg aactggggtt gaaactggat accggccggg
     5341 tgggcttgat gtgggattac gccgtccacg ccgccgctcc ggacggcggt tccttcggcc
     5401 tgcatgacag ttttgtctgg gataagccgt ccggcgaagg accggcggcc tggcagccgg
     5461 ccgccactcc gccgccggcc agccgggtca tggaacagcg gacggccttc ctggcggccg
     5521 ccggcctggc cgggaagccg gggtgggacc tggaccggcg gccggtccgg tacttcccgg
     5581 cggccggcca gtgcttcttc cggaccggtt gggaccggcg ggccaccggc ctggtctttg
     5641 acgccacccg ctgggggcgc ggccacatgc acctctcgcg cctcggtctt tcgctttatg
     5701 ccggggaccg gatgctcctc tacgacccgg gcatcttcag ttaccacatg aaggatccgt
     5761 tcgcctccta cggccggtcc accatctccc acaacacggt caacctggcc ggcttcaacc
     5821 agagcgacgc cgattgcgag gtgcgccgct gctgcctggg acgcggggcg gccgtcttct
     5881 cggccgccta cgagggcggc tactacccgg ggaagtacac ctggttcttc gacgacggca
     5941 agggaagcgg cctctacggc gcccacctgc ggaccgtcat ctgggtggac ggccggttca
     6001 tcctggtctg cgaccacctg cgcagcgacg gccgggggca gccctacgcg tcgcactggc
     6061 agttcccggc cggcccggtc cggctggatc cggccgggcg gcgggcctgg acactgggcg
     6121 aagacaacct gatgctccag gtgctggact ccatggatga actggaactc tccctgcacg
     6181 agggggagcg ggaaccgctg ctcggctggc tgcccgacca gagattggag gcgcatccgg
     6241 cgcccctgct ggccgtctcc ggccgggccg gccacccggc cgtcttctgg agcaccctcc
     6301 tgcttccctt ccggggcggt tcgcccccgg atttccgcct ggagagattc aagagttacg
     6361 gcgagtgcgg tttcggcttc cgcttcgact ggccggacgg ccgccgggat atcgtggcgc
     6421 tgacgccgcg gctggagcgg gccatcaacc tggtggagcc gctcggcccc ctggacaccg
     6481 acgccagcgt cgcggtcctc tccttccggc cggaccggaa gagctggcgg gccttcctga
     6541 tggatggcac ccggctgaac ttcgagggga aaccggttct ggaaaaggag aaggcggggg
     6601 tttacacggc ggtcttccgc ggctaaaatg aaacagacac cggcggaaat ttcaccaact
     6661 tcaaggagga gaagatggaa ggaagacgct tttcgccggg ccgcgcccgg ataccggccg
     6721 gcctcgttct gctgctgctg gccgcggcct gtttaccggc cggcctgtcc ggccagaccg
     6781 ccggcgcttc cggggcggcg gtgacccggg gaacttaccg gggtccggtc ttcgacggcc
     6841 tggccaccgt cgaaatttcg aacccggtct tgaaggtggt cacggttccg gagcgtggcg
     6901 gccgggtggc ccaggttatc cacctcaaga gcggccgtga tttcgtcaac ctttccgcgg
     6961 ccacggccct ggagaaggag aacggcatct acgaacggct ggacccgagc ggccgtcagc
     7021 cgggccgggc cgcgcccgac aacttcatgg gcatgccctt caaggccgag gcggccgggc
     7081 cgggcagcgt ccgctacacc tgccaggccg gttccttccg ggtggaacgg gtcctgaccc
     7141 tcgacccggc cctgccccgg ctggatgtcc gaaccaccta caccaatgtc gataccaagc
     7201 ccgtcacgac ccagtttttc ccgatctacc agttgaacac caaccaggac cggaccaccc
     7261 tctacgacgt gctggtcttt cccgattccc gggcgccggg cggcttcaag cgttacccgg
     7321 aaccgacccg cgaattccac tttgaaaccc agttcggctg gttcttcatc agcgacctgc
     7381 gctcccggga ggcggtggtc gtcacccacc gtcccaacga cccgatcatt gactccacct
     7441 atagctacaa ggtaaccacc ttccagcacc tggtggtcct ggggcgcgag caggaactgg
     7501 ccccccaggc ctcgatgacc ctggaggtcg gactctggtt catttccggc gccggcgagg
     7561 ttccggcgct ggccggagcg gccggcttga tcggtccgga gaatggccgc cggcttcagc
     7621 aggccctggc cgccgagctg gcccagccgg cgccgggcgc ggccgccttc gttctgccga
     7681 aggtggatga aaaggcgatc taccggccgc cggtcgagaa gccccggctg accctggccc
     7741 ggatcttctg ggacggcgtt tacgccaacc cggaaaggga ggatttcctc cagctgacct
     7801 cgtcccagta cctgctggag gcggtggccg aggtcaacgc catcaagccg gatctcctgg
     7861 ttatccaggg cggggcttac aacggcctgg agaaggagta tgccgatctg aagaagatca
     7921 ccggcctggc ccgggtgccg gtctcggtta cgcccggccg gcgggacaag ccggagaact
     7981 ggctgaagtc attcgattgg aattaccagc acctgcaggt cgagggtcag catctcttcc
     8041 tgctgccggc ctggagtttt ctcaaggccg acggccgcaa ggccctgatc caggagctgg
     8101 agaaggagct ggccgccctg gccggtacgg accgggcggt catcctctgt ccggatccgc
     8161 cgcgtctgcg ccagaacagc ggcaaggatg ttccggcggt cccggagctg cttgacctgg
     8221 tcaaccgcta cccggtcgtc ctctacctgt ccggttccgg caccaatctc ctccagcggg
     8281 atggaaaggc gacggtggtg ggcagttatg aactgaacaa cagccgctcc taccagctga
     8341 tccgtttcta tcccgaccgg atcgaggttg acctggccaa gccgctgggt gacaatttca
     8401 gcccggtggt gacggttccc tacgccccgg acctgaagcg gaccgccctt tacccgggcc
     8461 tgccggccgg ggagaagcgc cggccggatt acttctttgt ccacctttcc gacagccagt
     8521 tcggcgattc ggccgcccag cagaatccgg ccggccggaa cttcaacgat ttcggccgct
     8581 tcctgcaact gacctcggag accaatcggt tcaagccggc cttcctcatc aactccggcg
     8641 acctggtcga acggggcacc aacgaggagc agtggcggat ctacctgaag gcgacggcca
     8701 cttcgcgccg gccggtccat accgccatcg gcaaccacga cgccggaccg gagcattaca
     8761 cccgctggac gaaggagcag ccgctgcgcc tccagaagca ggggaaggat gccttcatct
     8821 acctgatgaa cgacacctcc agcgccagcc ggggcgaggg gttgagcccg gaactgatgg
     8881 cccgcctgcg ggtcctgctc gagaagagcc gttccgcccg ccagctcttc ctggtcaacc
     8941 acttcccgct ggacgcgatg cccggccagg cggccgagtt aaaggccctg ctggaggagt
     9001 accggcccac cctcttcatg gccggccata cccacaacct gggctacgaa tggcggggcg
     9061 agaccttcca ggcggtggcc aactccatcg gttggaccaa gcaggggccg aacacctcct
     9121 acaccggcta cttcatcaat tacgtctttc cggagcaggt ggtggtggtc tacaagccgc
     9181 tctacgaaga tgtcggctgg tcggtgacgg ttccgaatcc ccggcggaag aagtaggcgg
     9241 tgggtagatg aaaggacggc ggtcgaactg acccgaagaa gacataatgg gcggcggacg
     9301 aaaattaaac tggggcatca tcggctgcgg tgccctggcc gagcggcagc tggccccggc
     9361 catccaggcc ggggccaaca gccggctggc ggccgtctac agccgtgacc tggccagggg
     9421 ggaggatttc gcccggcgcc acggagccgg ccgggcctat gacgacctgg cggccttcct
     9481 ggccgacccg gaactggagg tggtttacat cggcacgccg ccccacctgc actgcgaaca
     9541 ggccgtggcc gccagccggg ccggcaagca catcctctgc gacaagccga tggccatgaa
     9601 cagcgccgag gcggccagaa tggtggaggc ggcccgggcc gccggcgtga aactgatggc
     9661 cggtttcatg atgcgtttca acgtctacaa ccggaaggcg gccgaactgc tccgcgccgg
     9721 cgccctcggg aaactcatcc acatccgcat ccaggcctcc tacaacctgc cggatacgag
     9781 tttcaactgg cgccaggacc cgacgctggc cggcggcggt tcgctctttg acatgggcag
     9841 ccacgcggtt gacctgctcc gcttcttcaa cggcgaaatc gcctcggcgc tggccttcgt
     9901 ggacaaccgg gcctatgcct acccggtcga ggataccgcc ctggccctgc tccgtttcgc
     9961 cggcggcggc tacgccaccc tggatgcctc cttcagcgag aaggcgcctt cccgcaacat
    10021 cgacatcttc ggcaaccagg gcagcctctg gctgaccggc tccatcggcc ggaacgccac
    10081 cggcgtgctt aacggctttc tcaacggcga aagaaaagag ttcaccctgg agccgaagca
    10141 catctatatt tccgaggtgg aggaactggg ccgggcgatc ctcgaggacc ggccgccgga
    10201 ggtgagcggg gaggacggct ggcgcaacct gctggtcctg gaggccatct accggtccgg
    10261 ccgggagggg cggctggtta acctttaaac gatattgtac aataaactac caaacctgat
    10321 tggaagagat ggggagcctg cggctgaaaa ggtttccgtc tcggtccgtc ccgttcctgt
    10381 cccggataaa atcaaggggg ttgaaatgat gcggaaatgg ccgatcgcct ttggttgtct
    10441 tctgctggcg gcgttccttt tccgggccgg gccggttctc gcccaggccg ggccgggcca
    10501 cctcttccgc ttcgcggcca tgggcgaccc ccagcccggc ctcgataatc ccggcaatta
    10561 cgggacgcac cttaaccagt tcaagcgggc gattcgtcag gtgaacgagt cgggggcgga
    10621 gttcacgatc attttgggcg acctggttaa caacgggcag gtggataccg agtacgagaa
    10681 catcgcggcg gccgcgaaga gtttcggcaa gccctggtac ccggtgcggg gcaatcacga
    10741 cgcgccggag aagttcaagc agtacttcaa atcggagtta aactactcct tcgagcacaa
    10801 agatttccgc ttcgtaatgc tggatgcggt cggcggccag gaggagctga cggtcgaaca
    10861 gctggcctgg gtggagaagg agttccaggc ggcgaagacg aagaagcagg agatcgtggt
    10921 ctgcacgcac gtttcggcct gggacaagaa tccgcgcggg gcctccccct acatgcagat
    10981 cggtcctgaa agcaaggcga agctgaagga gctttacaag aagtacgacg cgcttttgat
    11041 gctgtcgggc cactaccacc ggggtccctg gtacgcccag gaggagggca tgaactacct
    11101 ggtgctgccc ggaccggcca tgccccgtgg ttcgcccagc agttggcaga tcttcgatgt
    11161 ctacccggac cgggtggaga gttataccaa gcagatttac ctgccctacg acgacgagac
    11221 ggccgccggg tacatcaaca cgccctacct tacctgggtg gcctacggtc cggaacaggc
    11281 aagggtcttt gtgatgaagc gcaatgttaa atgaggatcc ggtttttcaa gccggccacc
    11341 cgccggcggt aacgcaggga ggagagatga tgaacgacag acgacgcttc tggatgcggc
    11401 tcggcctggc ggtaatgctg gccggcctgt tggccggtcc ggcctttgcc caggccggga
    11461 acgtgctctg gcaggaggat ttcgaggggt atgccgagaa cgcgcccctg gacggccaga
    11521 acggctggac gatgctcaag gtggccgacg cgccggcggc ggtggtgaaa tcgggtgccg
    11581 gcctgaaaaa ctccagggga ctggttctgt cgaataccaa ggccttccgg tccgattaca
    11641 tcggcctgac ccggactttc ccgcaggcgc tgaccgggac gctctggatc cagtgccgct
    11701 tcaaggcgcc ggccgcggcc gacaactgga tcggcggctt caatatcaaa agcaacctgg
    11761 ccggcttcag cgccgggctc ctccgggaga aggataagcc ggaccagatc cgcttcgtcg
    11821 tcccctactg gcagggatac cgcatccacc ggacggtccc ctacgagggg gggcgctggt
    11881 acacgttgac cctgaagctg gacaacgaac ggaagaccta cagcgcctgg gttgacgacg
    11941 tggaactggg cagcgatctg gacgtcggcg ccggcgaatt gaaatcagtt tacctgagtt
    12001 ccggcggcaa ggcggaggac ccggcggtcg ttgacgattt gattatcacc cggagccggc
    12061 cggccgggat caccgagcgg ccgctctacc cggccgggaa accgggccgc ctcttccgct
    12121 tcgcggccat gggcgatccc cagcccggcc tgggcaatta cgcggtcgaa ttgattcact
    12181 tccagcgggc ggtgaggcag gtgaacgagt cgggggcgga gttcacgctg atcctgggcg
    12241 acctggtcca cgacggcaag gtggatacca cctaccagga tatcgttgag acggcccggg
    12301 gcttcggcaa gccctggtac ccggtgcggg gcaaccacga cgcgccggag aagttcaaga
    12361 agtatttcaa gccggaattg aattattcct ttgagcataa agatttccgg ttcgtgatgc
    12421 tcgacgcgat cggcggccag gaggaactga cggccgagca gctggcctgg gtggagaagg
    12481 agttccaggc ggcgaaggcg aaaaagcagg agatcgtggt ctgcacgcac gtttcggcct
    12541 gggacgagaa cgcgcgcgga gtctcgccct acatgcagat cggtcctgaa agcaaggcga
    12601 agctgaagga gctttacaag aagtacgacg cgcttttgat gctgtcgggc cactaccacc
    12661 ggggtccctg gtacgcccag gaggagggca tgaactacct ggtgctgggc ggcaccggtt
    12721 ttgttcgttc ctcgccgacc ggctggtcgg tggtggacgt ctacccggac cgtttggaga
    12781 tctacacgaa accggtcttt tttccctgtg aggacgagaa gacaaccgaa ttttacgata
    12841 tgccctacct tgaatggcgt tcctacgagt cctaccggac cggccagggg gttgcggcca
    12901 agctccactt cccctatctg gttcggagtc cgctggtgat caagcggaaa gcgaggtaat
    12961 tgttaaaatt caaaaacagg ccggccttcc gccggctgga ggaggcattt aatgcttaag
    13021 ataaaccggg gttgttgcct ggcctttgcc gcgatatttc tggccggcct gttggccggc
    13081 ccggcttttg cccagacgaa gggaatcatc tggcaggagg attttgaggg ttacgccggc
    13141 gaggccgtct ttccggcccc ggccggcggc gaagtgaaga acggcctggt gaaggccggg
    13201 gtcggcttca agggcggccg ggcgctggcc gtcgacagtc tcaaggtcaa cgttccgctg
    13261 gcggcggggc tcgcggagcc ggtctgggtc cagtgccggg tcaaggccgc ggccggcagc
    13321 cgtcagctta acttgaatgt ttcaaccaac cttttcgtcg tctgctgggc gaaaccggtg
    13381 gaggcccaga aggagcagcc cgcttatacc cagctggtgg ttccttatta cgatggttac
    13441 cggatctata caccgctgaa actggatgac gcggagtgga cgaccctgac cttcaagctg
    13501 gatccggttc gccatgccca gaccacctgg gtgaacgaca cggtcatggt cggcgaatgc
    13561 gaccttccgg atcagccgct cctgagtgtc agcctcgcct tcgggggcgt cccggataag
    13621 ccggtcctgg ttgacgacct gtcggtgcgt accgtcaagc cggccgacct gcgggaccgg
    13681 ccgctctacc cggccgggaa gccgggccgc ctcttccgct tcgcggccat gggcgacccc
    13741 cagcccggcc tggagaatta caatgttgag ttacaccagt ttcggcgggc ggtgaagcag
    13801 gtcaacgagt cgggggcgga gttcacgctg atcctgggcg acctggtcca cgacggcaag
    13861 gtggatacca cctaccagga cctcgcggcg acggccaaga gcttcagcaa gccctggtac
    13921 ccggtgcggg gcaaccacga cgcgccggag aagttcaagc agtacttcaa atcggagttg
    13981 aactactcct tcgagcacaa agatttccgg ttcgtgatgc tcgacgcggt cggcggccag
    14041 gaggagttga cggccgagca gctggcctgg gtggagaagg agttccaggc ggcgaagacg
    14101 aagaagcagg agatcgtggt ctgcacgcac gtctcggtct gggacgacaa cctgcgcggc
    14161 gtctcaccct acatgcagat cggtcctgaa agcaaggcga aactgaagga gctttacaag
    14221 aaatacaatg ccttgctgat gctttcgggc cactaccacc ggggcccctg gcttcaccag
    14281 gaggagaaga tgagttacct ggtgctgccc ggaccggcct ggccgcggaa ctcgcccagc
    14341 agctggcaga tcttcgacgt ctacccggac cgggtggaga tgtacaccaa gcaggtcttc
    14401 ctgccctatg acgacgagac ggccaccggc ttcatcaaca ttccctacca gagctgggtt
    14461 aattatgaga cgcttcggac cgggaaggat gttccggcca agctccattt cccctacctg
    14521 gtccaggggc cgctggtgat caagcggaat gtcaggtaat ttttctttgg aaccgggccg
    14581 ccgtttccgc cggtaggttt cggaacctcc gctgtttcaa tcgaaccggg gataaggcat
    14641 gaaaaaatca gagatttgct ttgtttactt caacgacgcg ggcatgtgga ggcccagcga
    14701 cctggccggc cattttgaag acatgaagtc ggtcggcggg gagagcatct tcaccaactt
    14761 cctggagggt tcgcccgacg gcctggcctt ccggacccgg ctggcccacg cggccggcct
    14821 caaggtttac gcctctccgg ggcgcatcgc cggcctcttc gccgccgggc cgaggcccag
    14881 cagcatgttt cccgtccggc ggccggacac gctgatgcgc gacatcaagg ggcaaccggt
    14941 gctggccacc tcgggcctgg tctgctgcgt caacaacccc ggcttccagg agtggttctt
    15001 tccgcacatc gcggagattc ttcagaaaac gggcgtggac ggggtcgtct tcgacgagcc
    15061 gaaggaggcc cacatggcct gttactgcga gtactgccgg gccctggccg gggatgacgc
    15121 ggcggccctg gtggccctgc atgagaaggc gctggccgag acgatgggcc gggtcgccgg
    15181 ggagttcaag gccttccggc ccggcggcgc cgggctggtg atgctgatgc cgaacgccac
    15241 cgaaaacttc atcaaggagg tctgccggca gccggccatt gactgcgtcg gcgtggacgg
    15301 accactctgc ctgcagaact cccggccggt gacgccgccg caccagaaga cctatatcct
    15361 ggagagcgcg ccgcctttct tcaagctgat ccgggccgcc ggcaagcaga ccttcgcgct
    15421 ggccgagacc ttcggcgttt acgcctgggc cctggatgag ctgcgccgga acatcgggaa
    15481 gttcgcggaa ctcggggccg acgtggccgc cttcaactac tacggccacg acagcgacga
    15541 tccggaggcg gtaatggagg tggtgcgcga agcggcccgc ctggtgcgcg accgcggcac
    15601 ggaataaaac ttttcgattt tgaggtaccg gcctcgggcc ggcctccagg gaggatgaat
    15661 gccgaagaaa aaaaggttct ggcaactggc gggcctgctg atccttctct tggccgccgc
    15721 ggtcggtccg gcctttgccc aggccgggaa cgtgctctgg caggaggatt tcgagggtta
    15781 cgccgacaac tcagccctga acgggcagaa cggctggacg atgctgaaga cggccgacac
    15841 gccggcggga gtggtgaagg cggccgccgg gaccggcaac tccaagggcc tggccctttc
    15901 caacagcaag ggtttccgtt ccgattacat cggcctgacc cgtaacttcg ccgagccact
    15961 gaccgggacg ctttggatcc agtgccgctt ccggatgccg gcggccgaaa actggacggc
    16021 cggcttcaac ctgaagacca acctggccca ggtctccgcc ggcctggtcc aggagaagga
    16081 taagccggcc cggctccgtt tcgtcttccc ttactacgag gggaaccgca tctggcggga
    16141 gattcccttc gaagcgggcc gctggtacac ggtcaccctg aaactggaca ccgccggccg
    16201 gacctacagc ggttgggtgg acgggatcga gctgggtggc tacatcgaga tggccgggag
    16261 tgaattgaag tcggtctacc tcggagcggg cggccaggag ggcgccccgg ccgtcgttga
    16321 cgacctggtc gtcacccgga accggccggc cgggatcaat gaaaaaccgg cctacccggc
    16381 ccgggaggcc ggacatctct tccgtttcgc ggccatcggc gatccgcagc cgggcaagtg
    16441 gcgcatcaac aattaccagc acgaccttta caagctcggc cgggtggccg cccaggtgaa
    16501 cgaatcgggg gccgattttt cgatcgtcac cggcgacctg gtccatgacg ccgtctccga
    16561 tacgcccttc ctggacgccc tggaaaagct caaggttttg aagcgcccct ggtacccggt
    16621 gcggggagag caggagctgg tcgatctcta ccggaagcac atccggccgg agctgaatta
    16681 ctccttcgaa caccaggggt ttcgtttcgt gatgctggac gcggtcggcg gcgagacccc
    16741 cctggcggcc ggccagctgg attgggtgga gaaggagttc caggcggcgg cggccaagaa
    16801 gcaggagatc atcctctgca gccatgtctc accctgggac gagaaccggc tggccactgc
    16861 cccggcggac cggctggccc cggagagcaa ggcgcgcctg gtcgaactcc tgaagcgcta
    16921 ccgggtgctg atgacgctgg ccggccatta ccaccgcggc ctctggcatt tccaggccga
    16981 cggcgtcaat tacctggtcc tgccggtaac ctcgatggcc cgggtcagcc cggccgcctg
    17041 gaccgtcttc gacgtctacc cggaccgggt ggtggtgacg gtcaagcccc ttttcttcgc
    17101 cgccgaggat gccgaaacga aggagtttta cgatttcccc taccagacct ggcgcagtta
    17161 ccaggcgctc cgggccggcg ccgaaccaaa gagtcattat ccctaccagg ttggcgggcc
    17221 gctggtgatc aagcgcaccc gctgacagag aggagaagca tgaacggtct cgattggaag
    17281 gttggtctgc cttttctctt cttcctggcc gcctcgtcct taccggccgc cgcggataac
    17341 ggtccggtgc gcgatatcgg cgaccggctg gaattattcg tcgacgactg gctgatcgag
    17401 cggctggccg gcgccgagct tcgccttaac gagccggcgc tccgcgaggt ggtcttccgc
    17461 ttcgatgcgc ccggcgaggg ttccacctgc ctgccctcgg tcatcctgaa ggatggggac
    17521 cgctaccgga tgtactaccg gggcgagccg gtcaaggggc tggagaatac ccggtacgcc
    17581 gagagcgccg acggcatcac ctggagtcgg gtcaaggccg gcctctacga ggagaacggc
    17641 agccgggaga acaacatcgt ctttcgcgag ccggagaccc aggaactgga ggtcttcatc
    17701 gacggcaacc cggccgcgcc gaccggggag cgctacaagg ccttctgccg gtggaagggc
    17761 aaggatggcg agggacgggc ggtgcgggga atcaagatcc tggcctcggc cgacggaatc
    17821 cgctggcgcc tgctccagtc cgatccggtt atcagcctgc tccggcccaa ggagaccagt
    17881 atcctcgatt ccgacaaccg ggccttctgg gacgccgccc ggaaccgcta cgccggatac
    17941 ctgcgcgtct tccgggacgg gttccgcagc atcaagttct tcgcgtcgaa ggacttccgg
    18001 acctggggcg agccggagtg gctcgatttc ggcccggcgc cggccgaaca cctctacacc
    18061 aacgtcatcg gcccctactt ccgggcgccc catatcctgc tcggttttcc gaagcgcttc
    18121 ctgcccgacc gtaagttcct ggaggagtac cgctggaagg gtatctgcga tacctgcttc
    18181 atttccagcc gggacggccg caagtgggac cgccgcttca tggaggcctt cctgcgcccc
    18241 ggcgtcgatt cccgcaactg ggtttaccat tcgccgacgc tggcggccgg cggtccggtt
    18301 gagacggccc ccggggaact ctccttctat atcctggagc attacggcct gccggatgtc
    18361 cggctgcgcc gggcgaccat tcggaccgac ggtttcgtct cggtccgggc cggctttgcg
    18421 ggtggcgaac tgctcacccg tccgctgacc ttcagcggcc gggagctggt gctcaactac
    18481 gccacctcgg cggccggccg ggtccgggtc gaaatccagg acccggaagg gaaaccccgg
    18541 ccgggcttca ccctggagga ctgcccggag atctacgggg atcagctgga acggccggtg
    18601 gcctggaagg gcgggagcga tctctcggcc ctggccggcc ggccggtgcg cctccgcttc
    18661 cagctcaagg acgccgacct ctactcgatc cgtttccgcc ggtgaccggc ccgggttttc
    18721 aaggataacc acttgaggga gaaggtatga agaagatcgg ctgcgttaac attgacactt
    18781 cccacccgcg ctccttcggc gagtatttga agaagagcgg ccgggccggc tactacgcga
    18841 tctacaacga gggtttccgg ggcgacgacg aggtggccgg cttcatgaag acctacggcg
    18901 tcgagaagcg ctgcgccacg ctcgaggagc tggccgggag cgtcgacgcc tgtttcattc
    18961 acagctgcga ctgggaccgg aacctgcggc tggccgagcc gctgctcaaa ctcggcaagc
    19021 cggtcttcat cgacaagccg attgccggcc ggctgtccga ctgccggaaa ttggaggccc
    19081 tggccgccgc cggcgccgtc attctgggct gctcctcggt ccgctacgca cccgaggtgg
    19141 ccgaactggc cgggaaaccg gaggcggaac ggggccgctt tttgaccgtc ttcggcagcg
    19201 tcggggtgga cgagttcaac tatgccatcc acattgtcga ggccatcggc ggactgctgg
    19261 gcaccggcgc cgtctcctgc cgcttcctgg gccgggtcga ggcggagggg aagaagagcg
    19321 agaccttcct ggtccgtttc ggaagcgggg tcaccgcctt ctacaatacc tgcctgggtg
    19381 tcgggcagcc ttttgaaatg acggccgtga cgacccggac cacccaccac ttccgggtcg
    19441 acgccagccg gatctacggc gccctcctgg accggatctg cgattacctg gagaccggcg
    19501 tcaaccacct ggcgccggtg gcggccctga ccgagtcggt caagatcatg ctggccggcc
    19561 gcctctcccg ggaacggggc ggcgccgagg tcggactgga ggagatcccg gccgacgatc
    19621 ccggttttga cgggaccgcc ttcggggccg gttacgcggc cgcctccagg aagatttacg
    19681 cctgatgaag ccgccgctcc gggccgtctg cgccgagttc gggctgggcg cgccccgggg
    19741 tgcgatccgg ctggccggaa cccggaacct caattaccgg ctccggaccg agcggggcga
    19801 gtggctgttg cgccggcggt atgccggtta caaccagccg gcccggctgg atttcgacgc
    19861 cgcggccgcc cgttttctgg ccggggccgg cttgccggtg gcgccgccgc ttccttcccg
    19921 gagcggcgcg gccgcctgga gccggaaggg cgagcagtgg caggtatatc cctttttgcc
    19981 ggggcgtccc ctggttgacg gcgaccccgc ggatgcggcc gcgctcggcc gggccctggc
    20041 cgtctggcac cgtctggggc gcgatttcag tccggatttt gaaaagctcg ggacctgggg
    20101 tgaactcgcc ccgtctgaat tgagacgggt cctcgaacgg ctcgggggag agacgcccgg
    20161 cttggaagac cgcctcgatg attaccgggg cttgatggaa acgaccgccc ggcagctgga
    20221 ttcaaaggcc ggctggccct tgaccctggt ccacggcgat ctccagcccg ggaatatcct
    20281 ggtcgaggcg gaccgggtct cgggctttct cgatcttgac tggtgcggcc ggcgttcgcg
    20341 cctctacgac ctggcctttg tctttctctt ctgctgcgcc ggccacgacc ggccgctggc
    20401 cgggggcgac atctggacgc ttacccagcc gccccgctgg cggcggccgg ccttcgagag
    20461 ttgcgcggcg gcctattttt cagacgccgg cagccggctg acagcggcgg aggcggccga
    20521 gcttccggcc cgggtcaggc tggcctggtg ccacacgcgc ctgcacggcg ccctgaaggt
    20581 ggcccggggg cgccgccggt atttcctcgg ccgggagggt tggagcgatc cgcccgccga
    20641 tatttacaat ttttttacca ggggggagtg gccgagatga aaaggactcc gtttccgatt
    20701 cccaacaatc acgccgatta cgaactcgat cccagcccgg accggatcgg cgtaaagttt
    20761 gacaggccgc cggcggccgg ccgttatgtg actttcaagg ttacagtcgt cctggcggaa
    20821 ccgctcgagg cgggccaggg cctggcggtc ttttcgccct tcgggttcac cccaccgcag
    20881 ctccggacgc cgtcggccgc cggcttcgtg accgtcagcg gtccggccgg ggcggagctg
    20941 aagctgaccc attacctggt cggcaaggtc aagcgcgaac ggggcgtgat cgcctggccg
    21001 gtcacgaccg gtctgaagcg gggctcccgc ctggtcttta ccttcggcga ccggagccgg
    21061 ggcgggccgg gcagccgggt cagcctggtg gcccaccagg ggctcctggt ggccgggtac
    21121 cggctgcgca gccaggaaga ggcggcgccg cccctgctgg cgaggagtcc ccggatcgcg
    21181 gtgcggccgg accgcggcgc gctggtgcgt ggtttcgcca cgccggtgct ggccgcgggc
    21241 gaggaaggcc gcctgcagct ggtggccgag gaccgtttcg ggaaccgggc ggacgatttc
    21301 cgcggcgagg ttcgattgcg gggcgtcgaa aacctggccg gacttccgga taaggttgtt
    21361 ttcaaacccg ccgaccgggg tcgccgggag ctctccttcc gtccgcgccg gaccggctgc
    21421 ttcacgccgg tggttgtctc ggcccgggcc gaccacctgg ccggtccggt cgaggttacc
    21481 gaacggccgc ccgagtaccg gctttacttc ggagaactgc acgcccatac cgagttgagc
    21541 caggacggcg ccggttccct ggacgagttc tatgcctttg cccggtcggc ggccggactc
    21601 gatttcgccg ccgccaccga ccaccagctg ccggtggccg ggatgcccgg ttacgccagc
    21661 cacgccagcg gactgcccaa caccagccac gcgcgctacc cggaccgctg gcgggccacg
    21721 gccgaggccg cccggcgcca tcaccagccc ggccgcttcg tcacctttct cggtttcgag
    21781 ttcaacacct ccggcaatgc cggccaccgc aacctttact tcaagggaga cgagcccgaa
    21841 ataatcgaag cgccggcctg gccgctgccg gaacgcttcc tgcagtcctg ggccggccag
    21901 cgccgggata tcatggtggt cccccaccac ccggccatct gcttcagcgc cggggtctat
    21961 accacctaca aggggctgga gatcggcgtg gtggatgagc ggatccagcc gctggtcgag
    22021 atctactcga agcacggtac cagcgagtat ttcaacaatc cgcggccgct ccggggccag
    22081 gcggtcggcc atttcgtcca ggacatgctg gaggccggcg cccacttcgg cttggtcggc
    22141 ggcagcgacg gccaccaggc caaccccggc tccagcctcg aggagccggg ttggttccgg
    22201 acgctccagt accggagcgg cctggccgcg gtctgggccc gggaactgac ccgcgaggcg
    22261 ctctgggaag ccttcttcgc ccgccgggtc tatgccacca cctacccgcg ggtcatactc
    22321 cgtttccagg tcaacgacct cttcatgggc gcgatcggcc cggccgctta tccgcgccgg
    22381 ataaagatct tcgtggcctc gccgatcctg gtcaactacc tggaggtcat caagaacggc
    22441 cgggtggtct tcatgacccc ggagcagggg cagaaccacc ccatcccgcc ggaggtggag
    22501 ggcgaactgg agttcgttga cgaaaccccc tcggaccggg tcgaggatta ctactatctc
    22561 cggctgacgc tgcacggcag cgagcgggtc tggtccagcc cggtctgggt ttcgaatcaa
    22621 taagccagac aacgccggcg gccggcgggg catctaataa cttgaaaagc ggcccgggtg
    22681 aaacgcgggc gatttacctg acgaggagag agcatgaaaa aatcagtcat cggtattatc
    22741 tgcctgttta tcgccttgag ttcttcggct tttggccaga cggttccggc ggagcggaac
    22801 atcctctggg acggcggctt cgagctcggc tacgggaaca acttctgggg cacggtctac
    22861 gggaaccagg ggccggtctt gcgctcgatg tggtccggcg gggtgctgaa gctgaaccag
    22921 cgggtggcca gccgggttta tatcctggag gacgggacct acacgctctg cgcctgggtg
    22981 aaacgggctc cgggcttcga ggcccaggag agccgggtct ccctgctgct taccaacttc
    23041 aattactatc gggagacgca gaagaacctg tacggtaaaa ccttcccggt ggcggccggc
    23101 gccggctggc agcgggtagg ctggagcttc gaggtccagg gtccggtccg caatgatttt
    23161 cacgttgaga tctcaaccgg gaacccggcc gggtcggtgc tggtggacgg aatgagtctg
    23221 acccggggtt cggtgctgcc ggataagctg ccggcggccg cggcggtgga agcgggcttc
    23281 tggattccgg aggcgagcgg gatttacctg gacggcgagg agcgggcggc cgagctgatg
    23341 ataaggaacc acggcgcggc gcgtgcggcg cgggtgcgct gggagatcta cgatcaccag
    23401 gaggagctgg tgaagcgggg cgaggtgaac gagacgctgc cgg
//
LOCUS       DAOQYN010000006        21868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_2750_length_21868_cov_4.833494, whole genome shotgun sequence.
ACCESSION   DAOQYN010000006 DAOQYN010000000
VERSION     DAOQYN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21868
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2750_length_21868_cov_4.833494"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PKN80_00550"
     CDS             complement(<1..758)
                     /locus_tag="PKN80_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNQ34542.1"
                     /translation="MEGTQILRTRPRDSHKGDFGHLLILAGSPRFRGAPALAAAAALR
                     TGAGLVTLATGKSLVPGLQSGLTETMFLPLPEREDTIATAAALEELKGFLPRVEAVLA
                     GPGLGSEKAVAKLLHRFLPLCQQPLILDADALNALAGTPEFLKTIPGPVIITPHPGEF
                     ARLTGLPVADIQARRLETAQRFSRKFRVTTVLKGHQTVIAAPDGRHCLNLGGNPGLAT
                     AGTGDVLAGIIAGLVVQGLEAFTAARLGVCLHGLA"
     gene            complement(773..1144)
                     /gene="acpS"
                     /locus_tag="PKN80_00555"
     CDS             complement(773..1144)
                     /gene="acpS"
                     /locus_tag="PKN80_00555"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNQ34543.1"
                     /translation="MIRGIGIDIISVEKVARALSRQARFRNRVFTPPEQALCEQRPSP
                     ATHYAGKFAAKEAFLKALGREERRGIGWRDIEVLNTPGGQPYYRISGPAGAALAEAGG
                     KVLLSLSHSDGFAVAFCLIEK"
     gene            complement(1144..1395)
                     /gene="rpmA"
                     /locus_tag="PKN80_00560"
     CDS             complement(1144..1395)
                     /gene="rpmA"
                     /locus_tag="PKN80_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNQ34544.1"
                     /translation="MAHKKGQGSCKNGRDSSGKRLGVKRYGGQQVSAGEIILRQRGTQ
                     FKPGANVSMGKDYTLFALAAGKVTFASRRGQRVVEIQTA"
     gene            complement(1473..1545)
                     /locus_tag="PKN80_00565"
     tRNA            complement(1473..1545)
                     /locus_tag="PKN80_00565"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1510..1512),aa:Val,seq:tac)
     gene            complement(1603..2958)
                     /locus_tag="PKN80_00570"
     CDS             complement(1603..2958)
                     /locus_tag="PKN80_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNQ34545.1"
                     /translation="MEFNPTIFREYDLRGLAATDLKPGFARGLGRAFAGYLEAGAVPK
                     RVVVGHDNRLTSPGLTAELVEGLMAAGTEVIGLGQVPTPVFYFANQAQKTAAGIMVTA
                     SHNPPAYNGFKLVVNQHSLYGPQIQEIGRILAMDRFPSGRGSFRQETVLENYLAALRE
                     RTALKRPVRVVLDCGNGTAGPVALPALQSLGAEVIPLYCDSDPTFPHHLPDPLVPANL
                     KDLAARVRETGAEAGIGIDGDGDRIGVCDERGEMMWGDKILGLLIETALRARPGAPIV
                     YDVKCTLALEEEIRRLGGRPLMWKTGHSLIEQKMHEVGSPAAGELSGHLYFADRWFGF
                     DDAIYASARFLAYLSESQTPLSRLAEPLNTYLATPEIRLEVPEERKFDLVRDLERELA
                     AEFQVSTIDGVRVVFPDGWGLVRASNTQPALVLRFEARTAGRRDEIERLITGRLRRLL
                     A"
     gene            complement(2982..4124)
                     /locus_tag="PKN80_00575"
     CDS             complement(2982..4124)
                     /locus_tag="PKN80_00575"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNQ34546.1"
                     /translation="MKIDFTLRLPKKISFGAGRFETLAEEANLLAASGKVLCVLDPAV
                     RPVLWPRIEKTALAGRAVNLEIPAGEPTVEAVDRAAETARAVNPELVIGIGGGSAMDF
                     AKALAALIENPGSSEEYRGQDRLKKPCRPKIMVPTTAGTGSEISPTAVLIGGGRKGGI
                     NSPDLIPETALLDPELTLDLPAPVTVASGLDAFCHALESYLSVRSTPFSEPLSLQALE
                     LLVRGLPAALAAPGDLPARTDNLLGSLLAGITLASAGVIANHSLSYPLGARHGIGHGL
                     ANGIMLPYVVRACLPEAAEKLAKVADRLGVAPGASVSERAVRTADFIFEFEARLGFKH
                     RLRDLGVRAEELEAMAAEASQVAVPIANTPRPMNVAELAEIYRKAY"
     gene            complement(4121..5224)
                     /locus_tag="PKN80_00580"
     CDS             complement(4121..5224)
                     /locus_tag="PKN80_00580"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ34547.1"
                     /translation="METPLLNLKRQYQQLRPELEKALAAVFESQRFIRGPELKELESA
                     VADYHGIAHAVGVASGTDALLLLLEALDLKPGDEVITTPFTFVATAETILRAGATPVF
                     VDIDPATFNLDPNRLEAARTEKTRGVVPVHLFGLAADLDPILAWAEKNRLWVMEDCAQ
                     AFGAAYRGRKVGTFGVGGAFSFFPSKNLGGFGDGGLVTTADSRRAETVRILSEHGGRD
                     KYNVVMLGHNSRLDTLQAAVLLVKLRYLDEWNRNRRRLAAGYNRRLAGVGDLRLPAEP
                     EGSYHTYNQYTVRTGQRDRLQKFLAERKVGTAIYYPFALHQQELFRKTARIAGSLEQS
                     EATSREVLSLPIDPLLTEAEQDYVIEQVKEFFG"
     gene            complement(5341..6342)
                     /gene="waaF"
                     /locus_tag="PKN80_00585"
     CDS             complement(5341..6342)
                     /gene="waaF"
                     /locus_tag="PKN80_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNQ34548.1"
                     /translation="MVRRILVIKLGALGDLVLASAAFAALRKGFPTAELTLLTTPAYL
                     PLMRACPHFAAIRLFDRDRPFLPEAFRLRRELAGGGFDLAVNLQNNFKAGLLARLTGA
                     PVRAGFQKGLGLGLLNRPVRYAPAANALENLNRVLARAGAPAAAHLELYLRPETEAAG
                     REKLSRAGWKGEPLIGLHPGSSREWPAKRWPANYWARLSSAIEKRGWRPLFLGDRAAA
                     AETDRILAQTGTRALTLAGRTNLEELSALIKLCRAFVSNDSGPLFLAAAAGIRTIGLY
                     GPTAGKRHAPPGVTVLEAGLECQPCYRRDCPRPRCLESIPPEAVLRRLSEPATAVSR"
     gene            complement(6345..7445)
                     /locus_tag="PKN80_00590"
     CDS             complement(6345..7445)
                     /locus_tag="PKN80_00590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ34549.1"
                     /translation="MKIALLVPAMDTGGVEQGTYDLAAGFVRRGHQALVISGPGRFLP
                     RLEAAGVRHYLVPMERKNPVEFTAALRRVSKILRSERPDIIHARSRLPAWIAYLAGGR
                     RPPAGHFVTSIHGFHHTRWYSRIMGRGERVIVVSQGLKKYTAEFLGADPQLIRVVYNG
                     FDPEPFLKINPVPAGEKTEKNSFVIGAVGRLTEVKGYRYIIEAVALLKKPCPGLSATI
                     IGEGPEGGRLRELARRLGVADRVKFAAGQPAEHLASFQALAAPHLEPEGRPVDGPYWP
                     GRAAAEAQVAGRPVLTTLRELAPGQFEVGDACLFAAAGEATGLARGIEYLYRHPEEAA
                     ALAERGRRTALEHFSLEAMVEKTLAVYRELTA"
     gene            complement(7474..8247)
                     /gene="trpC"
                     /locus_tag="PKN80_00595"
     CDS             complement(7474..8247)
                     /gene="trpC"
                     /locus_tag="PKN80_00595"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNQ34550.1"
                     /translation="MKLEEILDGVRSRTAERAGRLGRPELERRAALSPVPRDFRAALT
                     GSRPPRIIAEIKRASPSRGPIRPGLDPVGTARGYQEAGAAAISVLTESGFFRGSLADL
                     AAVKTAVSLPVLQKEFILEEYQIFEGRAAGADAILLITAILPADRLAGLYRLSRDLGL
                     APLVEVHDERELETALALGAELIGINNRNLKSLEVNLETGRRLLSLIPPDRTAIIESG
                     LKTREKIGEFLRLGARAFLIGESLLSRPDPAAALREMLT"
     gene            complement(8244..9272)
                     /locus_tag="PKN80_00600"
     CDS             complement(8244..9272)
                     /locus_tag="PKN80_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNQ34551.1"
                     /translation="MKAACLAGIEKVTILERPAPEPGPGEVLVRVRTVGICGSDVHYF
                     KSGRIGDQVIIPPFTIGHEAAGEVAKLGTGVTGLEAGQPVAIEPAIACGQCEVCQRGR
                     PNLCAGVRFLGTPPIEGAYREYLVMPAANLFPLPKGLSLEEGALAEPLAVGLYAVNRA
                     RISPGETVAVFGCGPIGLSLILAARLAGAVVLAAEPLPARRAFALGIGADRVFDPQQT
                     DPVAEIMKETGGRGVDAAFEAAGQPTALRQAIETAALGGRVVMVGIPAEDDWQIPSHL
                     ARRKELDLLNIRRSAFCAEKIIRLMESGRLKAAGLVTHRFRLEELARGLKLVAGYGDG
                     VIKAVIEI"
     gene            complement(9335..9769)
                     /gene="dut"
                     /locus_tag="PKN80_00605"
     CDS             complement(9335..9769)
                     /gene="dut"
                     /locus_tag="PKN80_00605"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013979803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNQ34552.1"
                     /translation="MPSVPVPAKTSSADLPLPAYQHPGDAGLDLRAREAVRLKPGERR
                     LIPTGLRLALPAGTAGIIKDRSGLAFKHGLHTLAGVIDSNYRGEVKVLLINLGRRACR
                     IKAGDRIAQLIVIPCLEARLLPSEELPESERNESGFGSSGVL"
     gene            complement(9729..11939)
                     /locus_tag="PKN80_00610"
     CDS             complement(9729..11939)
                     /locus_tag="PKN80_00610"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNQ34553.1"
                     /translation="MKKEIEIGGKTISFDTETFAQQATASVMVRSGETMVLTTLVFGK
                     ELENRQDFLPLTVEYREHISAAGKFPGGFFKREGRPTEKEVLTCRLIDRTIRPLFPKT
                     LNREIQVCSFVFSFNPEVEPDMLAVLGASAAVTLGPIKAKDVVAGVRVGLVDGQLVVN
                     PSVTDLQKSELSLVVMAGTESLLMLEGSARNLPEDRVLEAVGFGEQEARKIVQFFKDE
                     FMPEKSALPEPEKQPWEDTVISILDSRFGEVTRYAQKVERAAFFEGIRQEVLAAVGPE
                     VAPKEVQGVFERELRTRVRRQILTSRVRMDGRQATQLRPISCQVGILPRVHGSALFRR
                     GETQALALITLGTWADEQRIEGLLEETRKRFTLHYNFPPFSVGETRPMRGPGRREIGH
                     GAMAERALNYSLPDEAEFPYTIRIVSDILESHGSSSMASVCAGSLGMMDAGVPVKEAM
                     GGVALGMIKEGEDYLILTDIAGEEDHYGDMDLKIAGTRNGITSIQMDVKTTQLSYEML
                     RQAFLQSRDARGQILDVMTATLPTHRPQLSPYAPRIETLKIPVDKIGAVIGPGGKNIR
                     RITEDTGAQIEIDDDGSVQIISKDDASREKAILIIRGMTEEPQVGQIYRNVKVSRILS
                     FGAMVEYLPGQEGLVHVSELDHRYVSKVEDIAKVGDTMDVKIVGIDDQHRVNLSRKAL
                     MEVPPGLDKENSSAQQARGPRRERSDFSGRQSGRPQPKLTHRAFRSGPGKDKQR"
     gene            complement(12164..12430)
                     /gene="rpsO"
                     /locus_tag="PKN80_00615"
     CDS             complement(12164..12430)
                     /gene="rpsO"
                     /locus_tag="PKN80_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNQ34554.1"
                     /translation="MLTAEEKTNLISSFHRHGKDTGSPEVQISLLTERINRLGDHFRT
                     HAKDHNSRRGLLVMIGQRRKLLSYLKRLNPSTYDKVISKLGLRK"
     gene            12573..13742
                     /locus_tag="PKN80_00620"
     CDS             12573..13742
                     /locus_tag="PKN80_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ34555.1"
                     /translation="MNYTFFIARRYFRRSRGGYLFLTSLFAIISVAVGVACLILVLGV
                     MNGFDRDLKRKIIGVQPAVTIQLPAGSGELAALERRLQAVPEVLAVSPFSETQAILRS
                     REYMIAVLVRAVDFKREDAVTGFGKYLDPARPDPGLVLGAELARSLRAGPGDQLQVIT
                     GFGMLPGRLPVTGRFQTGLYHFDASLAYLPLERGGALFPRGSRPEALGVKVADIYAAS
                     ETARRISRELGPEYKVESWIDRNQALFGALALERRAMAAILILIVMVAGFNIATSLMM
                     TVWRKSREIGTLRSLGVSRGGIRAIFLWMGFLTGLWGILGGLGVGLSLAYLGDRYRLI
                     KLPGYVYDLSYLPIQVRGTDVLWIVLSAFSIALLASIFPAQEASRVAPAVTLRSE"
     gene            13747..14421
                     /locus_tag="PKN80_00625"
     CDS             13747..14421
                     /locus_tag="PKN80_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ34556.1"
                     /translation="MELTGIGLQKIFGDGQSRVRALDGVDISLRPGEITLLLGPSGSG
                     KTTLLNLLSGLDEPTGGQVRFDGRPLSGLSDREKSLLRNRHFGFVFQFFNLLPEFTLL
                     ENVMLAGLIGGRSRNSAEARLRAVNLMERLGLADRLDARPSELSGGEQQRVAIGRALV
                     NRPDFVFADEPTGNLDRQNAENCLQLFRDLNREHGTAFIIATHNPEIAALLSSRVISL
                     KNGKIT"
     gene            14529..15407
                     /gene="ilvE"
                     /locus_tag="PKN80_00630"
     CDS             14529..15407
                     /gene="ilvE"
                     /locus_tag="PKN80_00630"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HNQ34557.1"
                     /translation="MGLLIYLDGKMLPESEAKVSVFDHGLLYGDGVFEGIRAYHGLVF
                     KLDEHVRRLYRSAATILLKIPIPPEEMALRILETLRANNLRDAYIRVVVTRGKGDLGL
                     DPNKCARATYFIIASRIQLYPPEFYEKGIEIITVPTRRNGVEALNPAIKSLNYLNNIL
                     AKIEGLNAGVHEALMLNQEGLVLECTGDNIFLVRGRDLVTPPIYLGVLDGITRQVVLG
                     LADAAGLQPRETPFTRHELFNADEVFMTGTAAEIVAVSKIDGRVIGSGRPGPMTQKLL
                     DRFRELTRKEGTPIHE"
     gene            15400..16983
                     /locus_tag="PKN80_00635"
     CDS             15400..16983
                     /locus_tag="PKN80_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34558.1"
                     /translation="MNEQEVRVKGVPRVAVVGAGYWGKNLVRVFHELGALGAICEINP
                     ALRAKFADAYPEPILDNFEAVLADPSLSAVVIAAPAVTHYQLARTALLRDKDVFVEKP
                     LALKVEEGEELVRLAGERGRVLMIGHILNYHPAVIKLKELINQGVLGRINYLYSNRLN
                     FGRIRTEENILWSFAPHDISVMLALLNEEPTQVTCRGAAYLQPEIFDLTLTHFTFQSG
                     AQAHIFVSWLHPYKEQKLVVAGSEKMALFDDATEEKLFLYHHRVAWEDRIPTALKASA
                     EPVPLEMTEPLRCEAEHFLQRLADRQPPRTDGREGLRVLRILDRCQSALTAPPAPAAE
                     MKTVRPYTVHPSAYVDEGVEVGAGTVIWHFSHLIRGSRVGRNCRIGQNVVIGPNANVG
                     DRVKIQNNVSVYEGVELEDYVFCGPSMVFTNVLNPRSAIPRMNELRRTLVRRGASLGA
                     NCTVVCGHTIGRYAFVGAGAVVTRDVPDYALVTGVPAKVTGWMCECGLKLDFRGKRTA
                     ACSCGLKYRKTGQSVEPLG"
     gene            17042..18067
                     /locus_tag="PKN80_00640"
     CDS             17042..18067
                     /locus_tag="PKN80_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ34559.1"
                     /translation="MGPDKAWVASAIAHRETEAVPYYFDFTPPARRRAESIYGSPLEE
                     RLCLPLRMGSCRKLKPLYADPSEFGPTARDEFGVTWSTSPVDRGAPIRPALSGPDLSG
                     YRFPDPAAAWRFEDIGAWSRRRRSQFTILWLGDLWERAAFMRGLEDLLLDLALHPGFV
                     EELLDGITGYLLDTLGILLERFEFDGVALSDDYGTQRSLLISPESWRRFITPRLDLIY
                     RPARAAGKIIFHHSDGNIWPIIPDLIELGCDILHPVQPEAMDVLRLKREFGRDLTFCG
                     GFPTQDLLVHGRPGQVRDEARRLMDLLGRGGGYIFSNGITIQSDVPADNLAALIDSAL
                     QGRSSSA"
     gene            18112..19407
                     /locus_tag="PKN80_00645"
     CDS             18112..19407
                     /locus_tag="PKN80_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNQ34560.1"
                     /translation="MTLKEKIKSRKARIGVIGMGYVGLPLAVNFLRAGFPVTGFDTDA
                     EKVARLCEGKTYIKHIPATLWTPYAGKFSATVDMKRLREIDAVVICVPTPLTANREPD
                     ISYVERAGRYIADSLDSVKLVSLESTTYPGTTDEVLRPILEAGGRRVGRNFYLVYSPE
                     REDPGNDAFSVRNIPKVVGGVTPACREHGLRLYSAIVERTVPVSSTRVAEATKLLENI
                     YRSVNIALVNEMKMLFDRMGINVWEVIEASRTKPFGFQAFYPGPGLGGHCIPIDPFYL
                     TWKARQYDFVTRFIELAGEINTGMPYYVIERTCEELNRRGRSLHKARVLILGVAYKKD
                     VDDMRESPALKIIRLFQERGARVSYNDPYIPRISNLRHYDFNLRSRSLTPRTLRESDA
                     VVIVTNHSRYDYHDILRHSRLVVDTRNAIPGRAPNLVKA"
     gene            19797..21461
                     /locus_tag="PKN80_00650"
     CDS             19797..21461
                     /locus_tag="PKN80_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNQ34561.1"
                     /translation="MVLAVFCVTVLLAGQAYGAPPSGDAVRQELESVPMLLSPTPEEP
                     LSVDLPYRGPMEVKDGQKIKVSRIVLRGNTVFGSTPLGALVSSCEGREVTLAELTEAA
                     NRITAYYQNAGYYFARAYVPAQEIKDGVVQIDVLEGFLGEIFINGNEKYTDCYVLRHL
                     CQLRKEGAIRYPTLERSLLLLNDNLGLKSKAVLKAGKELGTTDLYLELAESDPLLKGY
                     FDYNNFGSRYSSQHRFGFQVDFGNLTKHGDVLSLRGIVGSPIDQLFYGKINYALPVGC
                     YGTKAGVGFTTMRYEVGKEFKVLDMSGETTIYTLYATHPLLRSQSRNLNLEASFDHKS
                     VRNYVFSDFKTGDDTLSVFKVGANYDELRWGGRNYVSGFLHQGVPDFLGSLDSRDPES
                     SRGASGAGGDFTKLTLDLGHLRKIKDFAYLLVRAGAQVTSDDLVIAEQFSIGGPDSVR
                     GYPMSEYQGDKGYTLSAELRVPVFQSYWKDRVQVVGFLDYGEASLLTPLVGEDDKVCL
                     FGGGVGLRLSLPWETNVRVDLGFPLNSYEPSTGDSATWYFQVVKFF"
     gene            21631..>21868
                     /locus_tag="PKN80_00655"
     CDS             21631..>21868
                     /locus_tag="PKN80_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34562.1"
                     /translation="MERLIKNLRLVNFLLVIGLTLFGVPLPIFALPEGGQVVSGQASI
                     NVDGSNLTVTQGSNRAIVNWESFSIGEAELVQILQ"
BASE COUNT         3626 a         6779 c         7210 g         4253 t
ORIGIN      
        1 gccaggccgt gcaggcagac gccgagccgg gcggcggtga aggcctccag accctgaacg
       61 accagcccgg cgatgattcc ggccagcaca tccccggtcc cggcggtggc cagcccggga
      121 ttgcctccca agttgaggca atggcggcca tcgggagccg cgatcacggt ctggtgccct
      181 ttcaggaccg ttgtcacccg gaactttcgg ctgaatcgct gggccgtttc caggcgccgg
      241 gcctggatat cggcgaccgg gaggccggtc agacgggcga attcgcccgg gtgcggggtg
      301 atgataaccg ggcccggaat ggtcttgaga aactcggggg ttccggccag ggcgttaagg
      361 gcgtcggcat ccaggatcag cggttgctgg cagaggggca ggaaacggtg gaggagtttt
      421 gcaacggcct tttcggatcc gagtccggga ccggccagga ccgcttcaac ccgcggcagg
      481 aagcccttga gttcttccag ggcggccgcg gtggcaatcg tatcttccct ttccgggagc
      541 ggcaggaaca tcgtttcggt aagtccggac tgcagtcccg gcaccaggct tttcccggtg
      601 gccagggtaa ccaggccggc gccggtccgg agggcggccg ccgccgccag ggcgggggct
      661 ccccggaatc ggggcgagcc ggccaggatc agcaggtggc caaagtcccc cttgtgggaa
      721 tcccgggggc gggtccgcag aatctgcgtt ccttccatca gcacgggcct cgttactttt
      781 cgatcaggca gaaggcgacc gcgaaaccgt cggagtggga aagcgaaagg aggacctttc
      841 cgcctgcctc agccagggcg gccccggccg ggccggagat ccggtagtac ggctggccgc
      901 ccggagtgtt caggacttca atatcccgcc agccgatgcc gcggcgttcc tcacggccca
      961 gggccttcag gaaggcctcc ttggcggcga attttccggc atagtgcgtt gccggagaag
     1021 gccgttgttc gcagagcgcc tgctccggcg gggtgaagac gcggttgcgg aaacgggcct
     1081 gccgggagag tgcccgggcg actttctcga ccgagattat gtcgatcccg attccgcgga
     1141 tcatcaggcc gtctggattt ccaccacgcg ctggccgcgc cggctggcga aggtaacctt
     1201 gccggccgcc agggcgaaga gagtgtaatc cttgcccatc gaaacgttgg cgccgggttt
     1261 gaactgggtc ccgcgctgcc gcaggatgat ttcaccggcc gatacctgct ggccgccgta
     1321 acgcttgacg cccagccgct tgccggagct gtcccgcccg tttttgcagg aaccctgtcc
     1381 ttttttgtga gccatgaagc ctccttttgg ttgaaaagta ttttgtccgc ttctgagcgt
     1441 cgaaccgacc ggccgcttcc ggttcgatcg ggtgggcgag acaggacttg aacctgtgac
     1501 ctttcggatg taagccgaat gctccgccag ctgagctact cgccccaaat gatatatttt
     1561 actccaaccc cggcctcaag aacaagaaga acccggggtt tttcaggcga gcaggcgtcg
     1621 aagccggccg gttatcagcc gttcgatttc gtcgcggcgg ccggcggtcc tggcctcgaa
     1681 cctcaggacc agcgccggtt gggtattgga ggcccggacc agcccccagc catccgggaa
     1741 gacgacccgt acgccatcga tggttgaaac ctggaactcg gcggccagtt cccgctccag
     1801 gtcccggacc aggtcgaact tgcgttcctc ggggacctcc aggcggattt ccggcgtggc
     1861 caggtaggtg ttgagcggct ccgccagccg ggaaagaggc gtctggcttt cgctcaggta
     1921 ggcgaggaaa cgggccgagg cgtagatggc gtcatcgaag ccgaaccagc ggtcggcaaa
     1981 gtaaaggtgg cccgagagtt ccccggcggc cggcgatccc acctcgtgca tcttctgctc
     2041 gatcagcgag tggccggtct tccacatcaa cggccggccg cccaggcgcc ggatctcttc
     2101 ctccaaggcc agggtgcact tcacgtcata cacgatcggg gcgcccggcc gggcgcgcag
     2161 cgccgtttcg atcaggaggc ccaggatttt gtctccccac atcatttcgc cccgctcgtc
     2221 gcagaccccg atgcggtcgc cgtcaccgtc gattccgatg ccggcctcgg ctccggtctc
     2281 cctgacccgg gcggccaggt ccttgaggtt ggcgggcacg agcgggtccg gcaggtggtg
     2341 gggaaaggtc ggatcgctgt cgcaatagag cgggatgacc tcggccccca ggctttgcag
     2401 ggccggcagc gccaccggtc cggccgtgcc gttgccgcag tccaggacca cccggaccgg
     2461 ccgtttcagg gcggtccgtt cccggagcgc ggccaggtag ttttccagca cggtctcctg
     2521 gcggaaggaa ccccggccgg acgggaaacg gtccattgcc aggatccggc cgatctcctg
     2581 gatctgcggt ccgtaaagac tgtgctggtt gacgaccagt ttgaagccgt tgtaagccgg
     2641 cgggttgtgg ctggcggtca ccatgatccc ggccgccgtc ttctgcgcct ggttggcgaa
     2701 gtagaagacc ggcgtcggca cctggccgag gccgattacc tcggtcccgg ccgccatcaa
     2761 tccctcgaca agctcggcgg tcaggccggg cgaggtcagc cggttatcgt ggccgaccac
     2821 gacccgcttc ggaacggccc cggcttcgag ataaccggca aaggcccgtc ccaggccgcg
     2881 ggcgaaaccc ggcttgagat cggttgccgc caggccgcgc aggtcgtatt cccggaaaat
     2941 ggtcggattg aattccatgt tgtctctttc gggcggtttg cttaataggc cttccggtag
     3001 atttcggcca gttcggccac gttcatcggc cggggcgtgt tggcgatcgg tacggcgacc
     3061 tggctggctt cggcggccat cgcttcgagt tcctcggctc tgacgcccag gtcgcgcagc
     3121 cggtgtttga atccgagccg ggcttcaaat tcaaagatga aatcggcggt ccggacggcc
     3181 cgctcggaga cggaggcgcc gggtgccacg cccagccggt cggccacctt cgccagtttt
     3241 tcggcggctt cgggcaggca ggcgcgcacc acgtaaggaa gcatgatccc gttggccagg
     3301 ccgtggccga tgccgtgccg ggcccccagc gggtaggaga gcgagtggtt ggcgatcacc
     3361 ccggcgctgg ccagggtgat gccggccaaa agactgccca gaaggttgtc ggtccgggcc
     3421 gggaggtcgc cgggcgcggc cagcgccgcc ggcagaccgc ggaccagcag ttccagggcc
     3481 tggagcgaaa ggggttcgct gaaaggggtg ctgcggaccg agaggtatga ttccagggcg
     3541 tgacagaagg cgtccagccc gctggcgacg gtaaccgggg ccggcaggtc cagggtcagt
     3601 tccgggtcca gcagggcggt ttcgggtatc aggtccgggc tgttgatgcc gcccttgcgg
     3661 ccgccgccga tcagcaccgc ggtgggcgat atttcgctgc cggtgccggc ggtggtcgga
     3721 accatgatct tgggtcggca gggtttcttg agccggtcct ggccgcgata ctcctcgctg
     3781 ctgcccgggt tttcgatgag ggccgccagc gccttggcga agtccatggc gctgccgccg
     3841 ccgatgccga ttaccagttc cgggttgacc gcccgcgcgg tctccgcggc ccggtccacc
     3901 gcctccacgg tcggttcccc ggccgggatc tccaggttta ccgcccggcc ggccagggcg
     3961 gttttttcga tccggggcca gagaacgggc cggacggccg gatccaggac gcaaagaacc
     4021 ttgcccgaag ccgcgaggag attggcctcc tcggccaggg tttcgaaacg gccggcgccg
     4081 aagctgatct tcttgggcag acgcagggtg aaatcgatct tcatccgaag aactccttga
     4141 cctgttcgat cacgtaatcc tgttccgcct cggtcagaag cggatcgatc ggcagggaga
     4201 gcacctcccg gctggtggcc tcggattgct caagtgatcc ggcgatccgg gccgttttcc
     4261 ggaagagttc ctgctggtgg agggcgaagg gataatagat cgcggtaccg accttgcgct
     4321 cggccaggaa cttctggagc cggtcgcgct ggccggtgcg gacggtgtac tggttgtaag
     4381 tgtggtagct gccctcgggt tcggccggca gccgcaggtc gccaactccc gccagccggc
     4441 ggttgtatcc ggcggccagc cggcgccggt tgcggttcca ctcgtcgagg tagcgcagct
     4501 tcaccagcag cacggccgcc tggagggtgt ccagccggct gttgtggccg agcatgacga
     4561 cgttgtactt atcccggccg ccgtgttcgc tcaggatgcg gaccgtttcc gcccgccggc
     4621 tgtcggcggt ggtgaccagg ccgccgtcgc cgaagccgcc caggttcttg gaaggaaaga
     4681 agctgaaggc gccgccgacg ccgaaggtcc cgaccttgcg gccgcggtag gcggccccga
     4741 aggcctgggc gcagtcctcc atgacccaga gacggttttt ttcggcccag gccaggatcg
     4801 ggtccaggtc ggcggccagg ccgaagaggt ggaccggcac caccccgcgg gtcttttcgg
     4861 tccgggccgc ttccaggcgg ttcggatcga ggttgaaggt ggccgggtca atgtccacga
     4921 agaccggcgt ggcgccggcc cgcaggatgg tttcggcggt ggccacgaag gtgaagggcg
     4981 tggtgatgac ctcgtcgccg ggtttcaggt cgagcgcctc caggagcagc aggagcgcgt
     5041 cggttccgga ggcgacgccc accgcgtggg ctattccgtg gtagtcggcc acggccgatt
     5101 ccagttcctt gagttccggt ccgcggatga aacgctggct ttcgaagacc gctgccaggg
     5161 ccttctccaa ttccggccgg agttgctgat actgccgttt aaggttgagt agtggggttt
     5221 ccatggcctt tatgttaaca caaaagcggc ttcaggagaa aggtggaaag gaagaatgcc
     5281 gggatatcct gcttcgcctg gaagggccgg aatttccagg gccggccttc cggctcttgc
     5341 ctaacggctg actgcggttg ccggttcgga gaggcgccgc agcaccgctt ccggcgggat
     5401 gctttccagg cagcgcggcc ggggacagtc acggcggtag cagggctggc attccaggcc
     5461 ggcttccagg acggtcacgc cggggggcgc gtggcgtttg ccggcggtgg ggccgtagag
     5521 gccgatcgtc cggatgccgg cggcggcggc caggaacaag gggccgctgt cgttggagac
     5581 gaaggcccgg cagagcttga tcagggccga cagctcctcc aggttcgtcc gtccggccag
     5641 ggtgagggcc cgggtcccgg tttgggccag gatgcggtcg gtctcggcgg ccgcagcccg
     5701 gtcgcccagg aagagcggcc gccagccgcg tttttcgatg gccgaactca agcgcgccca
     5761 gtagttggcc ggccagcgct tggccggcca ctcccggctg gaaccgggat gcagtccgat
     5821 caggggctcg cccttccagc cggcccggct taacttttcc cggccggccg cctcggtttc
     5881 cggacgaaga tagagttcca ggtgggcggc cgccggcgcc ccggcccggg ccagcacccg
     5941 gttcaggttc tccagggcat ttgcggccgg cgcgtaacgg accggccggt tcaacagccc
     6001 caggccgagc cctttctgaa agccggcccg taccggcgct ccggtcaacc gggccagcag
     6061 gccggccttg aagttgttct ggaggttgac cgccaggtcg aagccgccgc cggccagttc
     6121 tcggcggagc cggaaggcct ccggtagaaa ggggcggtct cgatcaaaga ggcggatggc
     6181 ggcgaaatgc gggcaggccc gcatcagcgg caggtaggcg ggcgtggtca gcagggtcag
     6241 ttcggccgtc gggaagcctt tccgcagggc ggcgaaggcg gcgctggcca ggaccaggtc
     6301 ccccagggcg cccagcttga tgaccagtat cctgcggacc atggtcaggc ggtcagttcc
     6361 cgatagacgg ccagggtctt ctccaccatg gcctccagcg aaaagtgttc cagggccgtc
     6421 cgccggcccc gttcggccag agcggccgcc tcttccgggt gacgatagag gtattcgatt
     6481 ccccgggcca ggccggtcgc ttcaccggcc gccgcgaaca ggcaggcgtc acccacttca
     6541 aattggcccg gggccagttc gcgcagggtg gtcagcaccg gccggccggc cacctgggct
     6601 tcggccgccg cccgccccgg ccagtagggg ccgtcaaccg gccggccttc cggctccagg
     6661 tgcggagcgg ccagcgcctg gaaggaggcc aggtgttcgg ccggctggcc ggcggcgaac
     6721 tttacccggt cggcgacacc gagccgccgg gccagttccc gcagtcggcc gccttccggt
     6781 ccttcgccga tgatggtggc cgagaggccc gggcaaggtt tcttcaggag tgcgactgcc
     6841 tcgatgatgt agcggtatcc cttgacctcg gttaaccgtc cgacggcgcc gatcacaaag
     6901 gagttttttt ccgttttttc cccggccggg accgggttga ttttcagaaa gggttccgga
     6961 tcaaaaccgt tgtagaccac ccggatgagt tgcgggtcgg cgcccaggaa ttccgcggtg
     7021 tactttttga gtccctggct gaccacgatc acccgttcgc cgcggcccat gatccggctg
     7081 taccagcggg tatgatggaa gccatggatg ctggtgacga agtggccggc gggcggccgc
     7141 cggccgccgg ccaggtaggc gatccaggcc ggcaggcggc tgcgggcatg aatgatatcg
     7201 ggtcgctccg accggaggat tttagagacc cgcctcaggg cggccgtgaa ttcaaccggg
     7261 ttcttccgct ccatcgggac caggtaatgc ctgacgccgg cggcctcgag gcgcggcagg
     7321 aaccggcccg gcccggatat taccagggcc tggtggcccc ggcggacgaa cccggccgcc
     7381 aggtcgtagg tgccctgttc caccccgccg gtatccatgg ccggcaccag gagcgcgatt
     7441 ttcatcaggt tgtagccata acggcgtctt gtttcaggtc agcatttccc gcagggcggc
     7501 cgccgggtcc ggacggctca ggagggattc gccgatcagg aaggcccggg ctcccagccg
     7561 gaggaactca ccgatcttct cccgggtctt gaggccgctt tcgattatcg cggtgcggtc
     7621 gggcggtatc agggagagca gccgccggcc ggtctccagg ttgacctcga ggctcttcag
     7681 gttccggtta ttgatcccga tcagttcggc accgagcgcc agggcggtct ccagttcccg
     7741 ctcgtcgtgt acctcaacca gcggggccag gcccagatcc cggctgagcc ggtaaagccc
     7801 ggccagccgg tcggccggca ggatggcggt tatgagcagg atggcgtcgg cgccggccgc
     7861 ccgcccctca aagatctggt actcttccag gatgaactcc ttctggagaa ccggcaggct
     7921 gaccgccgtc ttgaccgcgg ccaggtcggc cagggaacct cggaagaagc cgctttccgt
     7981 gaggaccgag atggccgccg caccggcctc ctggtacccg cgggcggttc cgaccgggtc
     8041 aagtccgggc cggatcggtc cccgggatgg agaggctctc tttatttcag cgatgatccg
     8101 gggcggccgg ctcccggtca gggcggcccg gaagtcgcgg gggacagggc tgagggcggc
     8161 ccgccgctcc agttccggcc ggcccagccg gccggcccgt tcggcggtcc gggagcgcac
     8221 tccgtcgagg atctcttcca gcttcaaatt tcgatcacgg ccttgatgac gccgtccccg
     8281 taaccggcga ccagttttaa ccccctggcc agttcttcaa gccggaaccg gtgggtgacc
     8341 aggccggcgg ctttcagccg gccggattcc atcagtcgga tgatcttttc ggcgcagaag
     8401 gcggagcgcc ggatgttaag caggtccagc tccttgcgcc gggccaggtg gctggggatc
     8461 tgccagtcat cctcggccgg gatgccgacc atgaccaccc ggccgcccag ggcggccgtt
     8521 tcaatggcct ggcgcagggc ggtcggctgg ccggccgcct cgaaggccgc gtccacgccc
     8581 cggccgccgg tctccttcat gatttcggcc accggatcgg tttgctgcgg atcgaagacc
     8641 cggtcggctc cgatacctag ggcgaaagcc cggcgggccg gcaggggttc ggcggccagc
     8701 acgacggccc cggccagccg ggcggccagt atcagcgaga ggccgatcgg tccgcatccg
     8761 aagacggcca ccgtctcgcc cgggctgatc cgggcccggt taaccgcgta gagtcctacc
     8821 gccaggggtt cggccagggc cccttcttcc aggctcagtc ctttgggaag gggaaagagg
     8881 ttggcggccg gcatgaccag gtactcccgg taggcgcctt cgatcggcgg ggtgcccagg
     8941 aagcgcaccc cggcacagag attcggccgg ccgcgctggc agacctcgca ctggccgcag
     9001 gcgatggccg gttcgatggc caccggctgt ccggcctcca ggccggtcac cccggttccg
     9061 agctttgcta cttcgccggc ggcctcgtgg ccgatggtga acggcggtat gattacctgg
     9121 tcgccgattc tgcccgactt gaagtagtgg acgtccgaac cgcagatgcc gacggttctg
     9181 acccgaacca ggacctcgcc cggacccggt tcgggcgccg ggcgctccag gatggttact
     9241 ttttcaatgc cggcaagaca ggcggccttc ataagcttct ccccgggaaa cggcggcgtt
     9301 cgtgaaaaga gaaggggcgg ttttctttgg ttaattatag cacaccggaa gaaccgaatc
     9361 cggattcatt ccgttcgctt tccggcagtt cttcggaggg caggaggcgg gcttccaggc
     9421 aggggatgac tatcagctgg gcgatccggt caccggcctt gatgcggcag gcgcggcggc
     9481 cgaggttgat cagcaggacc ttgacttcac cgcggtaatt gctgtcgatg accccggcca
     9541 gggtgtgcag gccgtgtttg aaggccaggc cgctgcggtc cttgatgatg ccggcggtgc
     9601 cggcgggcag ggccaggcgc aggccggtcg gtatcaggcg gcgttctccc ggtttcagcc
     9661 ggaccgcttc ccgggcgcgc aggtcaagcc ccgcgtcgcc cgggtgctgg taagccggca
     9721 gggggaggtc agcgctgctt gtctttgccg ggaccggaac ggaaggcacg gtgggtcagc
     9781 ttgggttgcg gccggccgga ctggcggcca gagaaatccg aacgttcgcg gcgcggtccc
     9841 cgggcctgct gggccgagct gttctcctta tccaggccgg gcggcacctc catcagggcc
     9901 tttcggctca ggttgacccg gtgctggtcg tcgatgccca cgatcttgac gtccatggtg
     9961 tcgccgacct tggcgatatc ctcgaccttg ctcacgtagc ggtggtcgag ctccgaaacg
    10021 tgcaccaggc cttcctggcc gggcaggtac tcgaccatgg ccccgaagga aaggatccgg
    10081 ctcaccttga cgttccggta gatctggccg acctgcggct cctcggtcat gccgcggatg
    10141 atcaggatcg ccttttcccg cgaggcgtcg tccttggaga tgatctggac gctgccgtcg
    10201 tcatcgatct cgatctgggc gccggtatcc tcggtgatcc ggcggatgtt cttgccgccc
    10261 ggaccgatca ccgccccgat cttgtcgacc gggatcttca gggtttcaat gcgcggcgcg
    10321 tagggggaga gctggggccg gtgggtcggc agggtggccg tcatcacgtc cagtatctgg
    10381 ccgcgggcat cccggctctg caggaaggcc tggcgcagca tttcgtagga cagctgggtc
    10441 gtcttcacgt ccatctggat gctggtgatg ccgttgcggg taccggcaat cttaaggtcc
    10501 atgtccccgt aatgatcctc ctcgccggcg atgtcggtca ggatcaggta atcctccccc
    10561 tccttgatca tgccgagggc aacgccgccc atggcctcct tgaccggcac tccggcatcc
    10621 atcatgccca gcgaaccggc gcagacggag gccatcgagg aagagccgtg cgattccagg
    10681 atgtcggaga cgatccggat cgtgtagggg aattccgcct catccggcag cgagtaattc
    10741 aacgcccgtt cggccatggc tccgtgtccg atttcccggc gtccgggacc gcgcatcggg
    10801 cgggtttcgc cgacgctgaa gggcgggaag ttgtagtgca gggtaaagcg tttccgggtc
    10861 tcctccagca gtccttcgat ccgctgttca tccgcccagg ttcccagcgt gatcagtgcc
    10921 agcgcctggg tttcgccgcg ccggaagagg gccgaaccgt gcacccgcgg caggatgccg
    10981 acctggcagg agatcggtct cagctgggtg gcctggcgtc cgtccatccg gacccggctg
    11041 gtcaggatct ggcggcgcac ccgcgtccgc agttcccttt cgaagacgcc ctggacttcc
    11101 ttgggggcca cttcgggacc caccgcggcc aggacctcct gccggattcc ctcgaagaag
    11161 gcggcccgtt caaccttctg ggcgtacctg gtgacctcgc cgaagcggct gtccaggatt
    11221 gaaataaccg tgtcttccca gggctgtttc tcgggttcgg gcagcgccga cttctccggc
    11281 atgaattcgt ccttgaagaa ctggacgatc ttccgcgcct cctgttcgcc gaagccaacc
    11341 gcctccagaa ctcggtcttc gggcaggttg cgggcagacc cttccagcat cagcagcgat
    11401 tcggtcccgg ccatcaccac cagggaaagc tcgctcttct ggagatcggt gaccgagggg
    11461 ttgacgacca gttgcccgtc aaccaggccg acccggaccc cggccactac atctttggcc
    11521 ttgatggggc caagggtgac cgcggccgag gcgcccagga cggccagcat gtccggttcg
    11581 acctcgggat taaaggaaaa gacgaaggag cagacctgga tttcccggtt cagggtcttg
    11641 gggaagagcg gccggatggt ccggtcgatg agccggcagg taagcacttc cttctcggtc
    11701 ggccggccct cccgcttgaa gaatccgccc gggaacttgc cggcagccga aatgtgctcc
    11761 cggtactcca cggtcagggg caggaagtcc tgccgattct cgagctcctt gccgaagacc
    11821 agggtggtca ggaccatggt ctcgccggag cggaccatta ccgaggcggt cgcctgctgg
    11881 gcgaacgtct cggtatcaaa gctgattgtc tttccgccaa tttcaatttc cttcttcatt
    11941 tccattcctt tgcttgaatt gcttccgccg gacaaacagc cgataggtcc gttctgggcg
    12001 ggagaaacac cagtcaaaaa ttatctataa cgctcaaaca gaatgagctc aggagtgcgc
    12061 ttttttaaaa gaggggtatt tttccgatca aaatgcgatt tggtgaaaaa tgcggccttc
    12121 tttttctttt tcccgtcttt ttcctgtttt atcctgcccg tgattacttg cgcaggccaa
    12181 gcttggagat taccttgtcg taagtggagg gattcaacct tttcaggtag ctgagcagtt
    12241 ttcgcctctg gccgatcatg accaggagtc cgcgccgcga gttgtgatcc ttggcgtggg
    12301 tccggaaatg atctcccagc cggttgatcc gttcggtcaa gagggagatc tggacctccg
    12361 ggctgccggt gtccttgccg tgccggtgaa acgaactgat caggttggtt ttttcttctg
    12421 cggtaagcat tttacccgtg agattcgcgt tcttctggct tccgattttc ggagaaagta
    12481 taacccgggt tcgctttcgt tgtcaaattg gggtataatt cttcataaat tctttttcgc
    12541 caatccccgg ttccgccaga ttccgacaga gtatgaacta caccttcttc atcgcccggc
    12601 gttatttccg ccgcagccgg ggcggctatc tcttcctgac cagccttttc gccattatca
    12661 gcgtggcggt cggggtggcc tgcctgatcc tggtcctggg cgtgatgaac ggtttcgacc
    12721 gcgacctcaa gcgcaagatc atcggggtcc agccggcggt gacgatccag cttccggccg
    12781 ggtccggcga gctggcggcc ctggagcgtc ggctgcaggc agttccggag gtgctggccg
    12841 tctccccctt ttccgagacg caggccatcc tgcgttcgcg ggaatacatg atcgcggtcc
    12901 tggtccgggc ggttgatttc aagcgggagg acgccgtgac cggcttcggc aagtatctcg
    12961 atccggcccg gcccgatccc ggcctcgtcc tgggggctga actggcccgg agcctgaggg
    13021 ccggccccgg cgaccagctc caggtgataa ccgggttcgg catgctgccc gggcgcctgc
    13081 cggtgaccgg gcgcttccag accgggctct accattttga cgccagcctg gcctacctgc
    13141 ccctggagcg gggcggcgcc ctctttccgc gcgggagccg gccggaggcc ctcggagtca
    13201 aggtggccga catctacgcg gccagcgaaa cggcccgccg gatcagccgg gagctcgggc
    13261 cggagtacaa ggtggagtcc tggatagacc gcaaccaggc cctcttcggg gccctggccc
    13321 tggaacggcg cgccatggcg gccatcctga tcctgattgt catggtcgcc ggattcaaca
    13381 tcgccaccag cttgatgatg acggtctggc gcaagagccg cgaaatcggg acgctccgtt
    13441 ccctgggcgt cagccgcggc ggcatccggg cgatcttcct ctggatgggt ttcctgaccg
    13501 gtctttgggg aatcctgggc ggtctcgggg tgggcctttc gctggcctat ctgggagacc
    13561 gctaccgtct catcaagctg cccggctacg tttacgacct ctcctacctg ccgatccagg
    13621 tccggggaac cgacgttctc tggatcgtgc tgtccgcctt ttcgatcgcc ctgctggcct
    13681 ccatctttcc ggcccaggag gcctcccggg tggccccggc ggtaaccctt cgctccgagt
    13741 agccccatgg aactgaccgg aatcggactg cagaagatat tcggcgacgg ccagagccgg
    13801 gtcagggccc tggacggggt ggatatcagc ctgcgcccgg gggagatcac gctcctgctc
    13861 ggcccctccg gcagcggcaa gaccaccctg cttaacctgc tgagcggtct ggatgagccg
    13921 accggcggcc aggtccggtt tgacggccgc cctctttccg gcctttccga ccgggagaaa
    13981 tcgctcctgc gcaaccgcca tttcgggttc gtcttccagt tcttcaacct gctgccggag
    14041 ttcaccctgc tggaaaacgt gatgctggcc ggcctgatcg gcggccggag ccggaattcg
    14101 gccgaggccc gcttgcgcgc ggttaacctg atggagagac tcggcctggc cgaccgcctg
    14161 gatgcccgtc cttcggaact ttccggcggc gaacagcagc gggtggccat cgggcgcgcc
    14221 ctggtgaacc ggcccgactt tgtcttcgcc gacgaaccga ccggcaacct ggaccgccag
    14281 aatgccgaga actgcctcca gctcttccgc gatcttaacc gggaacacgg caccgccttc
    14341 atcatcgcca cccacaaccc ggaaatcgcc gcgcttcttt catctcgcgt catttccctg
    14401 aaaaatggta aaattacata ggtattcttc agttaaggaa cgcaacatca ccggcggcgg
    14461 cccggttcgg cccccgcagc ccggcttcag gccgggtttt taaatcaccc gaggcagggg
    14521 aaggtaacat gggacttctg atctaccttg acggcaagat gcttccggaa tcggaggcga
    14581 aggtttccgt ttttgaccac ggtctcctgt atggggacgg tgtcttcgag ggcatccggg
    14641 cctatcatgg cctggtcttc aagttggacg agcacgtccg gcgcctttac cggtcggccg
    14701 cgaccatcct gctgaagatc ccgattccgc ccgaggagat ggcgctccgg atcctggaga
    14761 cgctgcgggc caacaatctc cgcgacgcct acatccgggt ggtcgtcacc cggggcaagg
    14821 gcgacctggg cctggatccg aacaagtgcg cccgggccac ctacttcatc atcgccagcc
    14881 ggattcagct ttaccccccg gaattttatg aaaaggggat cgaaatcatc acggtgccga
    14941 cccggcgcaa cggggtcgag gctctgaatc cggccatcaa gtcgctgaat tacctcaaca
    15001 acatcctggc caagatcgag ggcctcaacg ccggggttca cgaagccctg atgctgaacc
    15061 aggagggatt ggttctggaa tgcaccggcg acaacatatt cctggtgcgt ggccgtgacc
    15121 tggttacccc gcccatctac ctgggcgttc tggatggcat cacccgccag gtggtgctgg
    15181 gtctggccga cgcggccggg ttgcagcccc gggagactcc ctttacccgg cacgagcttt
    15241 ttaacgccga cgaggtcttc atgaccggga ctgcggccga gatagtcgcg gtctccaaga
    15301 ttgacggccg ggttatcggt tccggccgcc ccgggccgat gacccagaaa ctcctggatc
    15361 gcttccggga gcttacccgg aaagagggga cgcctattca tgaatgaaca agaagtccgg
    15421 gtcaagggtg tgccgagggt ggcggtggtc ggggccggat actggggcaa gaacctggtt
    15481 cgggtctttc atgagctggg cgccctgggc gctatctgcg agataaaccc ggccttgcgg
    15541 gccaagttcg ccgacgccta tcccgaaccg atcctggata atttcgaggc cgtcctggcc
    15601 gatccgtccc tgtcggcggt ggttatcgcc gccccggcgg tgacccatta ccagctggcc
    15661 cggaccgccc tgcttcgcga caaggacgtc ttcgtggaga agccgctggc cctcaaggtc
    15721 gaggaaggcg aggaactggt gcgcctggcc ggcgaacggg gccgggtttt gatgatcggc
    15781 catattctga attaccaccc ggccgtcatc aagctcaagg agttgatcaa ccagggggtc
    15841 ctgggccgga tcaactacct ttactccaac cggctcaatt tcggccgcat ccggaccgag
    15901 gagaacatcc tctggtcctt tgccccccac gacatctcgg tcatgctcgc cctgctcaac
    15961 gaggagccga cccaggtcac ctgccgcggg gcggcttacc tgcagcccga gatcttcgac
    16021 ctcaccctga cccacttcac ctttcaaagc ggcgcccagg ctcacatctt cgtatcctgg
    16081 ctccatccct acaaggaaca gaagctggtg gtggccggca gcgagaagat ggccctcttt
    16141 gatgacgcca ccgaggagaa acttttcctt taccatcacc gggtcgcctg ggaagaccgg
    16201 atcccgaccg ccctcaaggc ctcggccgaa ccggtgccgc tcgagatgac cgaaccgctg
    16261 cgctgcgagg cggaacactt tctgcagcgc ctggcggacc ggcagccgcc ccgcaccgat
    16321 ggccgggagg gcctccgggt gctccggatc ctggaccgct gccagtcggc cctgaccgcg
    16381 cccccggccc ccgcggccga gatgaaaacc gtccggcctt ataccgtgca cccctcggcc
    16441 tatgttgacg agggagtcga ggttggcgcc ggcacggtca tctggcattt ttcccacctg
    16501 atccggggtt cccgggtcgg ccgcaactgc cggatcggcc agaacgtggt catcggcccc
    16561 aacgccaacg tgggcgaccg ggtcaagata cagaacaacg tgtccgttta cgaaggagtg
    16621 gaactcgagg attatgtttt ctgcggtccc tcgatggtct tcaccaacgt gctcaacccg
    16681 cgcagcgcca tcccccggat gaacgagctg cgccgaacgc tggtccggcg cggggccagc
    16741 ctcggagcca actgcacggt ggtctgcggc cacaccatcg gtcgctacgc cttcgtgggc
    16801 gccggggcgg tggtgacccg ggacgtgccc gattacgccc tggtcaccgg ggttccggcg
    16861 aaggtgacgg gttggatgtg cgaatgcggt ctgaagcttg atttccgcgg caagcggacc
    16921 gccgcctgtt cctgcggctt gaagtaccgg aaaaccggtc aaagcgtgga gccgctgggt
    16981 tgaccggggc ccggaccggg atcgccccgt tgaggcctcg gaaatcgcat cgctatcggt
    17041 catgggtccg gacaaggctt gggttgcctc ggcgatcgct caccgggaaa cggaagcggt
    17101 tccctactac ttcgatttca ccccgccggc gcgccgccgg gccgagtcaa tctacgggtc
    17161 tcccctggaa gagaggctgt gcctgccgct ccggatgggc agctgccgga agctgaaacc
    17221 cctctatgcc gacccgtccg aattcggccc caccgcccgg gacgaattcg gggtcacctg
    17281 gagcaccagc ccggttgatc ggggcgcacc catccggccg gcgctctccg gcccggatct
    17341 ctccggttac cgttttcccg acccggccgc ggcctggcgt tttgaagata tcggtgcctg
    17401 gtcccggcgc cgccgttccc agtttaccat tctctggctg ggcgacctct gggaacgggc
    17461 cgccttcatg cgcggcctgg aggaccttct gctcgacctg gccctccacc ccggttttgt
    17521 cgaggagttg ttggacggga taaccggtta cctgctggac accctgggaa ttctgctgga
    17581 acgttttgaa ttcgacggtg tggctttgag cgacgattac ggcacccagc gttcgcttct
    17641 gatctcgccg gaatcctggc gccgctttat cacaccccgg ctggacttga tttatcggcc
    17701 ggcccgggcc gccggcaaga ttatctttca ccattccgac ggaaatatct ggccgatcat
    17761 tcccgacctg atcgagctgg gctgcgacat tcttcacccg gttcaaccgg aagcgatgga
    17821 cgttctgcgc ctgaaacggg agttcggccg ggatctgacc ttctgcggcg gctttcccac
    17881 ccaggacctg ctggtccatg gccgccccgg ccaggtgcgg gacgaggccc ggcggctgat
    17941 ggacctcctg ggccgcggcg gcgggtatat attcagcaac ggcataacca ttcagtcgga
    18001 cgtgccggcc gacaacctgg ccgccctgat tgattcggca ctccaggggc ggtcgtcgtc
    18061 ggcctgaagt gaaaacgcca aacaagccct ttccccaatt atggagaagc gatgaccctt
    18121 aaagaaaaaa ttaaaagccg gaaagcgcgg attggtgtta tcggcatggg ttacgtcggc
    18181 cttccgctgg cggttaactt tctccgggcc ggttttccgg tcaccggttt cgataccgac
    18241 gccgaaaagg tggcccggct ttgcgaaggg aagacttata tcaagcacat cccagctacg
    18301 ctctggactc cttatgccgg caaattttcg gccaccgttg atatgaaacg gcttcgggaa
    18361 atcgacgcgg tcgtcatctg cgtgccgacc cccctgaccg ccaaccggga accggacata
    18421 agttacgtgg agcgggccgg ccggtatata gccgatagcc tggattcggt gaaactggtc
    18481 tccctggaat cgactaccta cccgggcacc accgacgagg tcctcaggcc gatcctggag
    18541 gcgggcggcc gccgggtcgg ccgtaatttc tacctggttt actctccgga acgggaggat
    18601 cccggcaatg acgccttcag cgtccgcaac atacccaagg tggtgggcgg cgtgaccccg
    18661 gcctgccggg agcacggtct gcggctttac tccgccatcg tcgaacggac cgttccggtc
    18721 tcctccaccc gggtggccga ggcgaccaag ctgctcgaga acatctaccg gtccgtcaac
    18781 atcgcgctgg tcaacgaaat gaaaatgctt tttgatcgga tgggcattaa cgtctgggag
    18841 gtgatcgagg cctcccggac caagcccttc ggcttccagg ccttttatcc ggggccgggc
    18901 ctgggcggac actgcatacc gatcgacccc ttctacctta cctggaaggc gcgccagtac
    18961 gattttgtca cccgttttat cgagctggcc ggcgagatca acaccgggat gccctactac
    19021 gtgatcgaac gcacctgcga ggagttaaac cgccgcggcc gctcgttgca caaggcccgg
    19081 gttctcatcc tgggggtggc ctacaagaag gacgtcgatg acatgcggga atcgccggcc
    19141 ctgaagatca tcaggctttt ccaggaacgc ggcgcccggg tttcatacaa cgatccctat
    19201 attccccgca tcagcaacct tcgccactac gatttcaacc tcaggtcccg ttccctcacg
    19261 ccgcggacgc tccgggagag tgacgcggtg gtgatcgtca ccaaccacag ccggtatgat
    19321 taccatgata tcctgcgcca cagccggttg gtggtggata cccgcaacgc cattccgggg
    19381 cgggctccca acctggtcaa ggcttgacat tgccattgcc gccgggtaga attacagtta
    19441 gagatgctct ttggaacttg acatttatct gtttgccggg tagaattcaa tacagagatt
    19501 atcaatattt gccttcgcgg ttgcaatgct tcttcggatc gggggaattg tgccaggaag
    19561 cggcgcatta atcattccgg ggggaacctg cttaccgttc attcttcatt ccgacggttg
    19621 ttgtccttcc tcaatcgtct catttcgccg gttcggaagt ccaaacttta ccgctcgctg
    19681 tcttccagag acctcaatgg tttcgttgct ttttttgccc tgagctttca ttttaattat
    19741 ttaaaaaagg atagggaggc gccatgcgtt tttgtgagag gcaacccgga ttagtaatgg
    19801 ttctggccgt attttgcgta acggttctgc tggccggtca ggcgtatggt gcgccgccgt
    19861 ctggtgatgc ggtgcgccag gagcttgaga gcgtgccgat gctgttgagt ccgactccgg
    19921 aggagccgtt gtcggtggac ctgccgtacc gtggtccgat ggaggtgaag gacggtcaga
    19981 agatcaaggt aagccggatt gtgcttcgtg gcaacacggt ttttggcagc acgccgcttg
    20041 gggcgctggt ttcttcgtgc gagggccgtg aggtgacgct ggcggagtta accgaagcgg
    20101 ccaaccggat cacggcttac tatcagaatg ctggttatta ctttgcgcgg gcgtacgtcc
    20161 cggcgcagga gatcaaggac ggggtggtgc agattgacgt actcgagggg tttcttggag
    20221 agattttcat caacggtaac gagaagtaca ccgactgtta cgttttgcgg cacctgtgcc
    20281 agcttcgcaa ggagggtgcg atccgctacc cgacgctgga gcggagcctg ctgctgctga
    20341 atgacaatct tggtttgaag agcaaggcgg tcttgaaggc cggcaaggag ttggggacga
    20401 cggacctgta tcttgagctg gcggagtccg acccgctttt gaaggggtac tttgactaca
    20461 acaatttcgg gtcgcgctac tcgagccagc accggtttgg tttccaggta gattttggga
    20521 acctgaccaa gcacggtgat gtgctgtcgc tgcgtgggat tgtgggttcg ccgattgacc
    20581 agttatttta cggcaagatc aactacgcgc ttccggtggg ttgttacggc acgaaggcgg
    20641 gtgtgggttt cacgacgatg cgttacgagg tagggaagga gttcaaggtt cttgacatgt
    20701 cgggcgagac gacgatttac actttatacg cgacacatcc gctgttgcgc agccagtcgc
    20761 gcaacctgaa ccttgaggcg tcgtttgacc acaagtcggt gcgcaactat gttttcagtg
    20821 atttcaagac gggcgatgac accttgtcgg tcttcaaggt gggcgccaac tacgatgagc
    20881 tgcgctgggg cggccgcaac tatgtatcgg gatttctgca ccagggggtg ccggactttc
    20941 tgggtagcct tgattcgcgg gacccggaat cgagccgggg cgcttcgggt gcgggcgggg
    21001 acttcacgaa gctgacgctt gaccttggcc atttgcggaa gatcaaggat ttcgcgtacc
    21061 tgctggtccg ggcgggtgcg caggtaacca gcgacgacct ggtgattgcc gagcagtttt
    21121 cgattggcgg accggacagc gtgcgtggat atccgatgtc ggagtaccag ggggacaagg
    21181 gctacacctt gagtgcggag ctgcgtgtac cggttttcca gagttattgg aaagatcggg
    21241 ttcaggtggt tggatttctt gattacggag aagcgtcgtt gctgacgccg ctggtgggag
    21301 aggatgacaa ggtatgttta tttggcggtg gggtgggttt gcgcctgtcg ctgccctggg
    21361 agaccaacgt gcgggtagac cttggatttc ctctgaacag ttacgagccc tcgacgggcg
    21421 acagtgcgac ctggtacttc caggtggtga aatttttctg acaatgagag gttaacggtt
    21481 aagaagtgtc ctctggtttg agggtggagg cgaagacagg ggcgatgatg gtccctgtgg
    21541 ttgcctctgc tctcaatgac gagggtagca agagatgaaa agattgccat tttcagattt
    21601 cagctatcga cattacaaag gaggggctcg atggaacgcc tgattaagaa tcttcggctg
    21661 gtaaattttc tgctggtaat agggttaact ctttttggtg tgccgttacc gattttcgct
    21721 ttaccggaag gcgggcaggt ggtgagcggc caggcgtcga ttaatgttga tgggagtaat
    21781 ctgaccgtaa cccagggcag caatcgggcg attgtgaact gggagagttt ttcgataggc
    21841 gaggcggaat tggtgcagat actccagc
//
LOCUS       DAOQYN010000007        19275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_3199_length_19275_cov_5.093080, whole genome shotgun sequence.
ACCESSION   DAOQYN010000007 DAOQYN010000000
VERSION     DAOQYN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19275
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3199_length_19275_cov_5.093080"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1119)
                     /locus_tag="PKN80_00660"
     CDS             complement(64..1119)
                     /locus_tag="PKN80_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34563.1"
                     /translation="MEPGVSRIFFAIGLIFSGFLFVFFSLLFGGAAVESAVPMAVLIT
                     GGLLLAGLSLFLFSLLRWLPLARWISSIPTSRKLRSVALGLSEVNGFADAFQPLISPA
                     TGRECCLTRWKQQELVRTHNARGGSTTSWKTIDSGLELAPFYLKDEEGCRILVDPEKA
                     RLDLPWETYYPGAAEGIFFQTEGSIRYLESAIYPRDYLYIMGEVRKRPGYQNIDLEVA
                     GELAVIRGDREKLDGYDRNRDGQVDPVEWDRAVADVERRVRGKIDRQAALEPLATLVI
                     ARPSVNRPFLIANRSEKDLLGRLLFKGYAGLAFGLALLFFNLAGLFGHFFHQPAFGEK
                     YFQVIKLIATAIQRGDG"
     gene            complement(1159..3543)
                     /gene="lon"
                     /locus_tag="PKN80_00665"
     CDS             complement(1159..3543)
                     /gene="lon"
                     /locus_tag="PKN80_00665"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNQ34564.1"
                     /translation="MARFTRKKKDPDPPDTNDLVTELKLKKVMPAVPLRDIVVFPGMV
                     VPLLIGRQKTINAVEEAQSQTKNLLVLVFQTHPETEDPAIKELIPIGTLARIIQVVRQ
                     PEGQLKLLVESLLRVRLDKAHPDLRYFAVSVEAAETGRRGNTEEEALQRLLVDSLENY
                     NQLNPVLPREILGNLLQINDPERLSDTLSGYLPLKKEDKARVLQELELVPRLKMLLQI
                     LNKEIEVLQVQRQIQGEVIKKIEKNQREYILQEQMKTIQQELGKEAEGSEVKAFQEKI
                     EASGMPEPVRKKALEELERFSKMMSLSPEATVVRNYLDWMVSLPWKKTTSDTLDIRKA
                     QEVLDEDHYGLEKPKERILEYLAVRKRSQSLKGPILCLVGPPGSGKTSLARSVARAMG
                     RQFIRVSLGGVRDEAEIRGHRRTYIGSLPGRVIQSMRKVGVRNPVFLLDEVDKLGMDF
                     RGDPASALLEVLDPEQNCAFNDHYLEVDFDLSQVFFITTANVEATIPPPLLDRMEIIT
                     LPGYTETEKLQIARRYLVPREMTANGLKDGEFEFSDEAIREIIRSYTREAGVRSLQRQ
                     IASICRKIVKEKELRDRKLSERVKVSRRRVGHYLGVPSYWNEESDRASRVGVVTGLAW
                     TEYGGDVLFTEVSTMKGRGQLIITGRLGEVMKESCRIALSLVRAHDEELGIDPEIYRR
                     QDIHIHVPSGAVPKDGPSAGVTIASALASCFSGRAVAGDIAMTGEITLRGKVMPIGGL
                     RAKLLAASRNQIRRVIIPKDNQRDLAEIPRAELAGLQIIPVENISEIFKIIFNN"
     gene            complement(3583..4197)
                     /locus_tag="PKN80_00670"
     CDS             complement(3583..4197)
                     /locus_tag="PKN80_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNQ34565.1"
                     /translation="MLIPYVIEATQTGERAYDIYSRLLKDRIIFIGTAIDEAVANLVV
                     AQLLYLQSEDPRRDIHLYLNSPGGIVTAGLAIYDTMQYIECDVATYCIGQASSMAVVL
                     LAGGAPEKRFILPNARVLIHQPWGGVQGDASEMSIQTREMLRLRDCINRLLARHTGQT
                     VRRISRDTDRDFYMSAEEAVSYGLCDRILDARPVTGSSAANQTK"
     gene            complement(4222..5211)
                     /locus_tag="PKN80_00675"
     CDS             complement(4222..5211)
                     /locus_tag="PKN80_00675"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNQ34566.1"
                     /translation="MPKTEFKKLEGCWRQIRVEVEPETVRGAWENILAGLQKEAQLPG
                     FRKGKAPLNLIRNHFDEHIQRELLNRLVPETYQAALEEHGLKPVADPRINDLELTGEK
                     LNYTAVFEVAPEVSLDSYSGLTLDNRKELNEAELDQALEEVLARNPQLQVQARDLKVR
                     ENLKENLRRRMELQIQSINERQEDERIIDQLLERATLQPPEGLVKNRTMELVRHELSH
                     QPDFKEKTLEAREALVKELMEKFQPLAEREVKASFVLSEVAAREKVEVTEADIDNRID
                     ILARLSGQEQEEFKHHFDAEHREDLRAQLQVEKTLALLKKRALLIEKPRIVKA"
     gene            complement(5296..5380)
                     /locus_tag="PKN80_00680"
     tRNA            complement(5296..5380)
                     /locus_tag="PKN80_00680"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5344..5346),aa:Tyr,seq:gta)
     gene            5502..6146
                     /locus_tag="PKN80_00685"
     CDS             5502..6146
                     /locus_tag="PKN80_00685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ34567.1"
                     /translation="MKRKLFAFDLDGTLLDAYPAIIASMNHTLARLSLPQAEPDTIVR
                     AVGLGNGGLIRAFVPESHFREAQRIYREDHLPRLSSDSRLLPGALELLERLKAHGKKL
                     AVATNRSRESADILLDTLAIRPYFDRVLVGDEVPNPKPAPDILLALLEHFGVAPAETL
                     YVGDMTIDAQTGAAAGVDTLIVITGSSSRAEVAAAAPYRIIDSLADFPAGEYRD"
     gene            6156..6482
                     /gene="cutA"
                     /locus_tag="PKN80_00690"
     CDS             6156..6482
                     /gene="cutA"
                     /locus_tag="PKN80_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNQ34568.1"
                     /translation="MKKDTGCLAVFVTCASLKEARKIARILVEEKAAACASLTAGVES
                     LYWWEGKIERAGEVLLSAKTTRTRFPRLAALVRQNHSYQVPEIIALPIAAGSRPYLDW
                     IEQSLR"
     gene            complement(6415..6933)
                     /locus_tag="PKN80_00695"
     CDS             complement(6415..6933)
                     /locus_tag="PKN80_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNQ34569.1"
                     /translation="MVKVLVVYYSRSGHTGEMAGFIARVALKRVEATSLEDLKAADAV
                     IIGSPTYYGGMAAPVKELLDRSVVFHKELEGRAGGAFASSAHVGGGNETTILSIINAL
                     LIHGMVVQGDPRSDHYGPVSVGAPDGRVEAECLRYGKRVTELAGRLRSVSGGSVRSSR
                     GRGVNRRRSAGR"
     gene            complement(7061..8641)
                     /locus_tag="PKN80_00700"
     CDS             complement(7061..8641)
                     /locus_tag="PKN80_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNQ34570.1"
                     /translation="MAKERWSSKLGIIMAVAGSAIGLGNFLRFPVQAAKHGGGAFLIP
                     YFIALLLLGIPLMWIEWTIGRFGGGFGHSTSPGIFQTLWNKNRFIKYFGVIGIFAPLV
                     IFIYYVYIESWLLGYSFFALTGLYNSCTTEEAMTLFLRGYQGLARNNFFSSLRPAYFF
                     FLVTFALNLLILYQGVQRGIERVCKYAMPVLFFLGIILLVRVFTLGTPNPQFPENSIY
                     NGLGFLWNPDWSALRSGRVWLAAAGQIFFTLSCGIGIILTYASYLRREDDVVLSGLSA
                     VATNEVAEVILGGSIVIPAAFAFFGPAGLARIAQGGSFNLGFVTMPLVLQRLGFGQAF
                     GFIWFGLLFLAGITSSISLAQPAIAFLEDEFNLGRRRTLALFAAVTFLLTNAVIFFMG
                     RGVLDEMDFWGGTFCLVLFATIETILFAWVFGIDRAWTEMHAGSDITLPRFYRFIIKY
                     VTPAFLLAILGFWLVQEGMPVIRLHGVPAGNVPYVTATRVLLLLIFAGLAWLVKKSWD
                     RRLKAGAAGSPEEEGRRI"
     gene            complement(8616..10997)
                     /gene="topA"
                     /locus_tag="PKN80_00705"
     CDS             complement(8616..10997)
                     /gene="topA"
                     /locus_tag="PKN80_00705"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNQ34571.1"
                     /translation="MKEGISLVVVESPAKCRTIGKYLGNDYRVVATMGHIRDLPADEL
                     GVNVEKEFEPTYRVMPRKQKVVSQVRKEAGSAAVIFLATDEDREGEAIAWHVAQLLKR
                     PAEEIKRVTFHEITPEAVRRAFNSPREIDLHLVNAQQARRVLDRLVGYTLSPLVGDLI
                     KKGLSAGRVQSAALRILVEREKEITGFKSEQYYLVTAEVARADKPEAVFAIQLAGRDG
                     KKISKPGIKDRGEAEQALAFLRANPLTVGQVTREERREFPDPPFTTSTLQQEAGRRLS
                     LPASRTMSLAQQLYEGIELGDQGPVGLITYHRTDSLNLAQSARDEAARYIKAEFGNQY
                     LPARPPVYRSRSSMAQEAHEAIRPTSTTRPPQSVAGYLSEGQLKLYTLVWNRFLASQM
                     APAVYELTRVRVDCGPYFLTTEGRVNRFDGHRRLAGFGQKDSVLPPLAEGDPLRLEKA
                     DLEEKATEPPPRYTEATLIKAMEKLGIGRPSTYAPIIETLVARLYVGREKRTLIPSPL
                     GVMVNDLLVSHFPEVIDLRFTAQMEDRLDLIAEGKEAWRQVLAEFYKPFLASVQAAEN
                     NMASSEKCPKCGKRLIFRKSKKFGAFLGCEGYPDCDFTKNISVTSSEVCPKCGKPLIL
                     RESKKFGSFLGCEGYPACDFTKNIGQAGRWHSRSSKPEAPAGAEGEVPAAPAPAEVCP
                     KCGKNLVLKKSKFGTFLGCEGYPACDFTRNLGKAGQRPAYRAKPSGSPGAGTVPCPKC
                     KTGRLVERQSRYGKFLGCSNYPRCRYIEKSGAGGPQPDASSNGGKDGEGAVEQ"
     gene            11460..11532
                     /locus_tag="PKN80_00710"
     tRNA            11460..11532
                     /locus_tag="PKN80_00710"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11494..11496,aa:Glu,seq:ctc)
     gene            complement(11637..13874)
                     /locus_tag="PKN80_00715"
     CDS             complement(11637..13874)
                     /locus_tag="PKN80_00715"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNQ34572.1"
                     /translation="MRSQSIRIVSFLFFALAGWLGLLQADPVSPLNGTADPDGNTVWY
                     DGARLGVEGRGWTETESFYDRLPSKAKTTVPAAIWELSHDTAGMCFRFVTDAPEIQVR
                     YKLLRKPEAMSHMSTRATSGMDLYCCDKNGKWVSVFAHGKPNSAQSPKKTEGFETAFY
                     NVDGSKEIRLYLPSYNGMKSVEFGIPRDRIISRPAPPADTRVKPVLFYGTSITQGASA
                     SRPGKAFPAIVGRSLDVPIINLGFAGGGRMEPELADLLGELDPSIYVLDCTADMEAEL
                     IMQRAAPFVMKLRQARPATPILLVEDPNCWNRSTVKGRALRSIYERLKKQGVTGLFFL
                     PNKGMWGDDGEATVDGVHPSDLGMMRQAAVFIEVLPRLLQTAGMGASKQRTASGAGKE
                     VPASAEVGVPPTPPDSSVKMADETDTVWYDGARFDVEGRGWTETESFYDRLPAKARGT
                     VRDSVWKLSHATAGMCIRFVTDAPTIQVRWIITGLYSLSTMPKTACSGVDLYCREAAG
                     KWTFVGTGEPTGITNTALFSVGASKELMLYLPLYTGVKSVEIGIPKGRTISKPAPRAK
                     PIIFYGTSITQGACASRPGMVYTAIVGRRLDRPVINLGFSANGTGDTELADLMGEIDA
                     SIYIVDCAANMKSNGPYAPEKQAPLFVTRLRKLRPATPILLVQGADRFDRPSEGGAWR
                     SVYEQLKKQGMTGLFFLPNKGMYGDDGEATGSDGHPSDLGMMREAAVYIRALPSMLKT
                     TGRSR"
     gene            14264..14743
                     /locus_tag="PKN80_00720"
     CDS             14264..14743
                     /locus_tag="PKN80_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34573.1"
                     /translation="MCDNSPEAGEKKGENPDDKQKYIQLVYRSALEDIRFFKSQQWLV
                     TYYTLLVYWVLVAAAKSINYGAWLSCLVPVVLVMSCALICQLEHSIKEQRKIKDEAIE
                     KIKTVSEEIKDLFPNLPTTFSELPLFILLSMTILGGCVTVWIITNLPGFCQAAGCRR"
     gene            complement(14749..15873)
                     /locus_tag="PKN80_00725"
     CDS             complement(14749..15873)
                     /locus_tag="PKN80_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34574.1"
                     /translation="MVKAISPEGRFCFFGYYDKCGWDESGEYHLFHEVDFQDRPPAAG
                     DRARICLLETKTGRVEVLDETPAWNFQQGAMLQWLPDRRIIYNSVRGGDFISVIHDLK
                     SGRRSELPRPVSAVSRDGRLALSLNYARLARWRPGYGYEGVADPFEHQKWPEADGVYL
                     VDLETGGHRLVISLAGLLELRPEPETRDSFGWVNHTLFSPDGRRFIFLNRWKSGPKER
                     HRTRIFTANPDGSGLHDLVETQLVSHFDWKDGRELLAWMEYGGKSGFWLIDDRTGHGR
                     FLSENIQFLDGHCSYSPDRKFILLDTYPQAGQRSLKLFDTVRDREITTGEYFSPPEIT
                     GPIRCDLHPRWGRPPNLISFDSAHEGYRRVYAADIGEYLG"
     gene            complement(15912..16814)
                     /locus_tag="PKN80_00730"
     CDS             complement(15912..16814)
                     /locus_tag="PKN80_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009677190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34575.1"
                     /translation="MSELKIGMVGLDTSHAVSFTKLLNFPGAPFHVPGGRVTAAFPGG
                     SQAFSMSRDRVPQFTSQFKDEFGVEICDSIESVMKQVDALFLESVDGRQHLEQFRVIA
                     PFGKPVFIDKPFTCSAADAHEIIALSKQHRAPIFSASAMRYAAGVAGIEQGRRVFGCI
                     AFGNMKILPDYPGLYWYGVHSAEVLFSKLGSGCRQVFVQKGEAADAVTGLWEDGRTGV
                     LYGYRVDNVPQQFGCSVLTDGGLVHGIVQAEPPTYALILEKIFDFFKTGKSPVDLQET
                     LEIMAFLEAANRSRESGRPEKLAL"
     gene            17021..17093
                     /locus_tag="PKN80_00735"
     tRNA            17021..17093
                     /locus_tag="PKN80_00735"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17054..17056,aa:Asp,seq:gtc)
     gene            17387..18727
                     /locus_tag="PKN80_00740"
     CDS             17387..18727
                     /locus_tag="PKN80_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNQ34576.1"
                     /translation="MEERILLSEKEIPQQWYNLLTDLKTPLAPPRHPATGEPLKPEDL
                     YPIFPKSLVAQEVSSERWIEIPDPILEIYRLWRPTPLCRARRLEKALGTKAQIWYKYE
                     GASPAGSHKPNTAIAQAYYNKQEGVTRLSTETGAGQWGSALSLACQLLGLKCTVYMVK
                     GSYEQKPYRRIMMETWGAEVFASPSDKTESGRRIQVEMPGTPGSLGIAISEAVEDAAT
                     HEDTKYSLGSVLNHVCLHQTVVGLETKKQFKLAGLKPDILIGCVGGGSNFAGLVFPFV
                     PEKLAGQEMRFIAVESAACPSLTRGQFAYDFGDTAGLTPLLKMFSIGHRFVPAPVHAG
                     GLRYHGMAPMVSHLMAEKLIEARAYQQKEVFEAALLFARTEGIIPAPETNHALKAAID
                     VARECREEKVILINFSGHGHFDLSAYEAFLTGRMTDSTVSDETLNKSMGDLKKI"
     gene            18749..19135
                     /locus_tag="PKN80_00745"
     CDS             18749..19135
                     /locus_tag="PKN80_00745"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HNQ34577.1"
                     /translation="MERILLKKWVVLLVMAVAVTISLRPASLQAAVVPAQTATASGNV
                     NQILDNPEVMSRLAALGYTRAEVRETLVSMTPAQRADLEDRVSLLSRGGNGLGVIVVI
                     LVIVVFIALLLEASGHRLSIETKAGN"
BASE COUNT         3684 a         6011 c         5956 g         3624 t
ORIGIN      
        1 aaggggtatg ccggcctcgc cttcgggctg gcgctcctct ttttcatcgc ttcctccttt
       61 tgtctacccg tcaccccgct ggatagcggt cgctatcaat tttatcacct ggaaatactt
      121 ctctccaaag gccggctggt ggaagaagtg gccaaagaga ccggccaggt tgaaaaagag
      181 gagcgccagc ccgaaggcga ggccggcata ccccttgaag agcagccggc cgagcaggtc
      241 cttctcgctc cggttggcga tcaggaaagg gcggttgacc gacggacggg caatgaccag
      301 agtggccagc ggttccaggg ccgcctgccg gtcgatcttg ccgcggaccc gccgctcgac
      361 atcggccacg gcccggtccc attccacggg gtcgacctga ccgtcccggt tccggtcgta
      421 accatccagt ttctcccggt cgccccggat caccgccagt tcgccggcca cctcgagatc
      481 gatgttctgg tagcccggac gcttgcggac ctcgcccatg atgtaaaggt aatcgcgcgg
      541 gtaaatggcc gattccaggt agcggatgct gccctcggtc tggaagaaaa taccctcggc
      601 cgcccccggg tagtaggtct cccagggcag gtccagccgg gccttctccg ggtcgaccag
      661 gatccggcag ccctcctcgt ccttgagata gaagggggcc agttccaggc cactgtcgat
      721 cgtcttccag ctggtggtgc tgccgccgcg cgcattgtgg gtgcgaacca gttcctgctg
      781 tttccagcgg gtcaggcagc actcccggcc ggtggccgga ctgatgagcg gctggaaggc
      841 gtcggcgaag ccgttgacct cggacaggcc gagggcgacg gagcggagtt tccgtgaggt
      901 ggggatggaa gaaatccagc gggccagcgg cagccagcgc aggagggaaa agagaaagag
      961 cgacaacccg gccagcaaca gcccgccggt gatcaggacc gccatcggga ccgcggattc
     1021 cacggcggct cctccgaaaa ggagtgagaa gaatacgaac aggaacccgg agaagatcag
     1081 gccgatggca aagaaaatcc tcgacacccc tggttccatg gcccctccgg ggagcggccg
     1141 ccgccggctt cccggccgtt aattattgaa gatgatcttg aaaatctccg agatgttctc
     1201 caccgggatg atttgcagac cggccagttc ggccctgggt atctcggcca ggtcgcgctg
     1261 gttgtctttg ggaatgatga cccgcctgat ctggttgcgg gaggccgcca gcagcttggc
     1321 acgcagcccg ccgatgggca tgaccttgcc gcggagcgtg atctcgccgg tcatggcgat
     1381 atcaccggcc accgcccggc ccgaaaaaca ggaggccagc gccgaagcaa tagtcacgcc
     1441 ggccgaaggc ccgtccttgg gaaccgcccc cgacggcacg tggatgtgga tgtcctgccg
     1501 gcggtagatc tccggatcga tacccagctc ctcgtcgtgg gcccgcacca ggctcagggc
     1561 gatgcgacag gactccttca tcacctcgcc cagccggccg gtgatgatga gctggccgcg
     1621 ccccttcatg gtgctgacct cggtaaagag gacatcgccg ccgtactcgg tccaggccag
     1681 gccggtcacc acgcccaccc gggacgcccg gtccgattcc tcgttccagt atgagggcac
     1741 gcccagatag tggccgaccc ggcgccggct gaccttgacc cgttccgaca atttccggtc
     1801 gcggagttcc ttctccttga cgatcttccg gcagatcgag gcgatctgcc gctggaggct
     1861 gcgcacgccg gcctcgcgag tgtaggagcg gatgatctcc cggatcgcct cgtcggaaaa
     1921 ctcgaattcc ccgtctttca agccattggc ggtcatctcg cgaggcacca ggtagcgccg
     1981 ggcgatctgg agcttctcgg tctcggtgta gccgggcagg gtaatgatct ccatccggtc
     2041 cagcaggggc ggcgggatgg tcgcctcgac gttggcggtg gtgatgaaga aaacctggga
     2101 aaggtcgaaa tcaacctcaa gatagtggtc gttgaaggcg cagttctgtt cgggatccag
     2161 cacctccagc agggccgagg ccgggtcgcc ccggaaatcc atgcccagct tgtccacctc
     2221 gtcgagcagg aagaccgggt tgcgcacgcc gaccttgcgc atcgactgga tgacccggcc
     2281 gggcagggaa ccgatgtagg tgcgccggtg gccccggatt tcggcctcgt cccggacgcc
     2341 gcccagggag acgcggatga actggcggcc catggcccgg gccacggaac gggccagcga
     2401 ggtcttgccg ctcccgggcg gcccaaccag gcagaggatc ggccccttga gcgactggct
     2461 gcgcttgcgc acggccaggt actccaggat ccgttccttc ggcttctcga gcccgtagtg
     2521 gtcctcgtcg agcacctcct gggccttgcg gatgtccagg gtgtcgctgg tcgtcttctt
     2581 ccagggcagg ctgaccatcc agtcgaggta attgcggacc acggtcgcct cgggcgaaag
     2641 gctcatcatc ttggagaatc gttccagttc ctccagcgcc ttcttccgga ccggttccgg
     2701 catgccgctg gcctcgatct tctcctgaaa ggccttgacc tccgatccct cggcctcctt
     2761 gcccaattcc tgctggatgg tcttcatctg ctcctgcagg atgtactcgc gctggttttt
     2821 ctcgatcttc ttgatcacct cgccctggat ctggcgctgg acctgaagga cctcgatctc
     2881 cttgttcagg atctggagca gcattttcaa gcgcggcacc agctcgagct cctgcagaac
     2941 ccgggccttg tcttccttct tcagcggcag gtagccggag agggtgtcgg agaggcgttc
     3001 cgggtcgttg atctgaagca ggttgcccag gatctcgcgg ggcagaaccg ggttgagctg
     3061 gttgtaattc tccagggaat cgaccagcag gcgctggagt gcctcctctt cggtgtttcc
     3121 ccggcggccg gtctcggccg cttcgacgct caccgcaaag tagcgcaggt ccgggtgggc
     3181 cttatccagg cgcacccgca ggagcgactc caccaggagt ttcagctgcc cctcgggctg
     3241 gcggaccacc tggatgatgc gggccagcgt cccgatcggg atcagttcct tgatggcggg
     3301 gtcctcggtc tccgggtggg tctggaagac cagcaccagg aggttcttgg tctggctctg
     3361 ggcctcttcg accgcgttga tcgtcttctg gcggccgatc agcagcggca ccaccatgcc
     3421 cggaaagacg acgatgtccc gcagcggcac cgcgggcatc accttcttca gcttgagctc
     3481 ggtgaccagg tcgttggtat ccggcggatc gggatctttt ttcttgcggg tgaatcgggc
     3541 cattgttctc caatcttgat gttcgcggaa accgggaaaa acctactttg tttgattcgc
     3601 ggcggagctt ccggttaccg gacgggcatc aaggatgcgg tcgcagagcc cgtaactgac
     3661 ggcctcttcg gccgacatgt agaaatcacg gtcggtgtcg cgggagatgc ggcgcaccgt
     3721 ctggccggtg tgccgggcca gcaggcggtt gatgcagtcg cgcagcctga gcatctcccg
     3781 ggtctggatg gacatctcgg aggcgtcacc ctggacgccg ccccagggct ggtggatgag
     3841 gacccgggca ttgggcagga taaaacgctt ctcgggggcg ccgccggcca gcaggaccac
     3901 cgccatgcta ctggcctggc cgatgcagta ggtggccacg tcgcactcga tgtactgcat
     3961 ggtatcgtag atagccaggc cggcggtcac gattccgccc gggctgttca ggtagaggtg
     4021 aatatcccgg cgcggatcct cgctctgcag gtaaagcagc tgggccacca ccaggttggc
     4081 caccgcttcg tcaatggcgg tgccgatgaa gataatgcgg tccttcagaa ggcgggagta
     4141 aatgtcgtaa gcccgctcgc cggtctgggt cgcctcgatg acgtacggta tcagcatgcc
     4201 gccccccgtt tcggagactg gttaagcctt gacgatcctg ggcttctcga tcagcagggc
     4261 ccgtttcttg agcagggcca gcgtcttctc gacctgcagc tgggcccgga gatcctcccg
     4321 gtgctcggcg tcaaaatggt gcttgaactc ctcctgttcc tgtcccgaaa gccgggccag
     4381 gatatcaatc cggttgtcga tgtccgcctc ggtcacctcg accttctccc gcgccgccac
     4441 ctcggacagg acgaaggagg ccttgacttc ccgttcggcc aggggctgga atttttccat
     4501 caattccttg accagcgcct cccgcgcctc gagggtcttc tccttgaaat ccggctggtg
     4561 cgagagttcg tggcgcacca gttccatggt ccggttcttc accagaccct cggggggctg
     4621 cagggtggcc ctttccagga gctggtcgat gatccgttcg tcctcctggc gctcgttgat
     4681 ggactgaatc tggagttcca tccgccgccg caggttctcc ttcaggttct cccggacctt
     4741 gaggtcgcgg gcctggacct ggagctgcgg gttgcgcgcc aggacctctt ccagggcctg
     4801 gtcgagttcc gcctcgttca actccttccg gttatccagg gtcagtcctg agtacgaatc
     4861 gagggacacc tccggggcaa cctcgaagac cgcggtgtaa ttaagtttct cgccggtcag
     4921 ttccaggtca ttgatccggg gatcggccac cggcttgagt ccgtgctcct ccagggccgc
     4981 ctggtaggtt tccggcacca accgattcag aagttcgcgc tggatgtgct cgtcgaaatg
     5041 gttccggatc aggttcagcg gggccttgcc cttccggaat cccggcagct gggcctcctt
     5101 ctggagcccg gccaggatgt tctcccaggc gccgcgcacc gtctccggtt ccacctcgac
     5161 ccggatctgg cgccagcacc cttccagttt tttgaattcg gtcttcggca tgtttcggct
     5221 ctctcttctt tcggaacgtt aagaatggac cggcgggcca cccgccggct gggctgaaga
     5281 aaaaatcgct tgtcgtgggc agggaaggat tcgaaccttc gtagaccttt cggtcgccag
     5341 atttacagtc tggtgcgatt ggccgctccg ccacctaccc gttaaaacca gctggtaaag
     5401 ggccaaattt cggctaaatt ttatcttttt ccgggttttt tgtcaaaaat aacggatttt
     5461 ttgaccgtaa acaaaggggt agggtaaaat aatcgctggc catgaaaaga aaactcttcg
     5521 ccttcgacct ggacggaacc ctgctggacg cctacccggc aattatcgcc agcatgaacc
     5581 ataccctggc gcgcctcagc ctgccccagg ccgagccgga cacaatcgtc cgggcggtgg
     5641 ggctgggcaa tggcggcctg atccgggcct tcgtgcccga gagccatttc cgggaggccc
     5701 agcggatcta ccgggaagac catctgcccc ggctgtccag cgactcccga ctgcttcccg
     5761 gcgcgctcga actcctggag cggctcaagg cgcacgggaa gaaactggcc gtggccacca
     5821 accggtcgcg cgaaagcgcc gacatcctgc tggacaccct ggccatccgc ccctactttg
     5881 accgggtgct ggtcggcgac gaggttccca atccgaaacc ggcccccgac atcctgctgg
     5941 ccctcctgga acacttcggg gtggcgccag ccgaaaccct ctatgtgggc gatatgacca
     6001 tcgatgccca gaccggcgcg gccgccgggg tcgacaccct gattgtaatc accggctcct
     6061 cctcccgggc cgaagtggcc gccgccgccc cttaccgcat catcgactcg ctggcggact
     6121 tcccggccgg cgagtaccgc gattgacacc gcctgatgaa aaaagatacc ggctgcctgg
     6181 cggtcttcgt cacctgcgcc tcgctgaaag aggcccggaa aatcgcccgg attctggtgg
     6241 aggagaaggc ggccgcctgc gccagcctga ccgccggagt ggagtcgctc tactggtggg
     6301 aggggaaaat cgagcgggcc ggagaggtgc tcctgtcagc caagaccacc cggacccgtt
     6361 tcccgcgcct ggccgccctg gtaaggcaga accactccta ccaggtcccg gaaatcatcg
     6421 ccctgccgat cgccgccggt tcacgcccct acctcgactg gatcgaacag agcctccgct
     6481 gaccgaccgc agccttccgg ccaattcggt tactcttttc ccgtaacgga ggcattccgc
     6541 ctcgacccgc ccgtcgggcg ccccgactga aaccggtccg taatgatcgg aacggggatc
     6601 cccctgaacc accatcccgt gaatgagaag cgcgttaata atggagagga tggtcgtctc
     6661 gttcccgccg ccgacatggg ccgatgaagc gaaggcgccg ccggcccggc cttccagttc
     6721 cttgtgaaaa accacgctcc ggtcgagcag ctccttgacc ggggcggcca tgccgccgta
     6781 ataggtgggc gaaccgatga tgacggcgtc ggcggccttg agatcctcga gggaggttgc
     6841 ctcgacccgc ttcaaggcga cccgggcgat gaagccggcc atctcgccgg tatgaccgga
     6901 acgcgagtag taaacaacca ggaccttgac catgctcatg tccttaccgg ggactggtcc
     6961 cgcggcccct aattgttgcg caggctccgg cgcagcgtgt agatacaggt gatcagcagg
     7021 gccagccagg cgatcgccat gaacaggatg ccgctcggcc tcatatccgc ctcccctctt
     7081 cctccggact tcccgccgcc ccggccttga ggcgccggtc ccaggatttt ttgaccagcc
     7141 aggccagccc ggcgaagatc aggagcagga gcacccgggt cgccgtgacg tagggaacgt
     7201 tcccggccgg aaccccgtgc aggcggatga ccggcatccc ctcctgaacc agccagaaac
     7261 cgagaatggc cagcaggaag gccggcgtca cgtacttgat gataaatcgg tagaaacggg
     7321 gcagggttat gtcggaaccg gcgtgcatct cggtccaggc ccggtcaatg ccgaagaccc
     7381 aggcgaagag gatggtctcg atggtggcaa agagcaccag gcagaaggtg ccgccccaga
     7441 aatccatctc gtccagtacg ccccgcccca tgaagaagat caccgcgttg gtcagcagga
     7501 aggtcaccgc ggcgaagaga gccagggtgc ggcgccggcc gaggttgaac tcgtcctcga
     7561 gaaaggcgat ggccggctgg gccagcgata tcgaggaggt gatgccggcc aggaagagca
     7621 ggccgaacca gatgaagccg aaggcctggc cgaaacccag gcgctgcagg accagcggca
     7681 tggtgacgaa gccgaggttg aaggagccgc cctgggcgat gcgcgccagg ccggccggcc
     7741 cgaagaaggc gaaagcggcc ggaatcacga tgctgccgcc caggatgacc tcggccacct
     7801 cgttggtggc cacggcgctc aacccggaga ggaccacgtc atcttcgcgc cgcaggtagc
     7861 tggcgtaagt caggatgatc ccgatgccgc agctgagcgt aaagaagatc tggccggcgg
     7921 ccgccagcca gacccggccg ctgcgcaggg ccgaccagtc cgggttccag agaaagccca
     7981 gcccgttgta gatgctgttc tccgggaact gcgggttggg cgtccccagg gtgaagaccc
     8041 ggaccagcag gataatgccg aggaaaaaga gcaccggcat cgcgtacttg cagacccgct
     8101 cgatgccccg ctggacgccc tggtaaagga tcaggaggtt gagcgcgaag gtgaccagga
     8161 agaagaagta ggccgggcgg aggctggaga agaagttgtt gcgggccagg ccctggtagc
     8221 cccggaggaa aagggtcatc gcctcctcgg tggtgcagga attgtagagg ccggtgaggg
     8281 cgaagaagct gtagccaagc agccaggact cgatgtagac gtagtagatg aagatgacca
     8341 gcggcgcgaa gatcccgatc acgccgaagt acttgatgaa acggttcttg ttccagagcg
     8401 tctggaagat accgggggag gtgctgtggc cgaaaccgcc gccgaagcgg ccgatggtcc
     8461 actcgatcca catcaacggg atgcccagca gcagcagcgc aatgaagtaa ggtatcagga
     8521 aggccccgcc tccgtgcttg gccgcctgga ccgggaaacg cagaaaattg ccgaggccga
     8581 tggcgctgcc ggccaccgcc atgatgatgc caagcttact gctccaccgc tccttcgcca
     8641 tccttgcctc cgttgctgga agcatccggc tgaggaccgc cggcgccgga cttttcgata
     8701 taacggcacc gcggatagtt gctgcaaccc aggaacttgc cgtatcggct ctggcgctcg
     8761 accagccgcc ccgtcttgca cttcgggcag ggaacggtcc cggcgccggg ggacccggac
     8821 ggcttcgccc ggtaggcggg gcgttggccg gccttcccga gattcctggt aaagtcgcag
     8881 gccgggtacc cctcgcagcc gaggaaggtc ccgaacttgc tcttcttgag cacgagattc
     8941 ttgccgcact tggggcagac ctcggccggg gccggggcgg ccggaacctc gccttcggct
     9001 ccggccggcg cctccggttt cgaagagcgg gaatgccagc gcccggcctg gccgatattc
     9061 ttggtgaagt cgcaggccgg gtacccctcg cagccgagga aggagccgaa cttcttgctc
     9121 tcccgcagaa tgagcggttt gccgcacttg gggcagacct cgctggaggt gaccgagatg
     9181 ttcttggtga agtcgcagtc cgggtacccc tcgcaaccga ggaaggcccc gaacttcttg
     9241 ctcttcctga agatgagccg cttgccgcac ttgggacatt tttcgctgga agccatgtta
     9301 ttctcggcag cctggaccga agccaggaaa ggtttgtaga actcggccag cacctggcgc
     9361 caggcctcct tcccctcggc gatcaggtcg agccggtcct ccatctgggc ggtaaaccgg
     9421 aggtcgatca cctccgggaa gtgtgacacc aaaaggtcgt tgaccatgac gcccagcggc
     9481 gagggaatca gggtccgctt ctcccggccc acgtagaggc gggccaccag ggtttcgatg
     9541 atcggggcgt aggtcgaggg gcggccgata ccgagcttct ccatcgcctt gatgagggtc
     9601 gcctcggtat aacggggcgg cggctcggtc gccttctcct ccaggtcggc cttctcgagg
     9661 cgaagcggat cgccctcggc caacggcggc aggaccgaat ccttctggcc gaagccggcc
     9721 agccggcgat ggccgtcgaa acggttgacc cgtccctcgg tcgtcaggaa ataagggccg
     9781 caatccaccc gcacccgggt cagctcgtag accgccggcg ccatctggct ggccaggaaa
     9841 cgattccaga ccagggtgta aagcttcagc tgcccctcgc tcaggtaccc ggccacgctc
     9901 tgggggggac gggtcgtgct ggtggggcgg atggcctcgt gcgcctcctg ggccatcgaa
     9961 ctcctggaac gatagaccgg cggccgggcc ggcaggtact ggttgccgaa ctccgccttg
    10021 atataccggg cggcctcgtc acgggcgctc tgggccaggt tgagcgaatc ggtccggtgg
    10081 taggtgataa gcccgaccgg cccctggtcg cccagctcga tgccctcgta aagctgctgg
    10141 gccagggaca tcgtccggct ggccggcagg cttaaccggc ggcccgcctc ctgctgcagg
    10201 gtggaggtgg tgaagggcgg atcggggaat tcacgccgtt cctcgcgggt gacctggccg
    10261 accgtgagcg gattggcccg caggaaagcc agggcctgct cggcttcgcc ccggtccttg
    10321 atgccgggct tggaaatttt cttgccatcc cggccggcca gctgaatggc aaagacggcc
    10381 tccggcttat cggccctggc aacctcggcc gtgaccaggt aatactgctc cgacttgaag
    10441 ccggtgatct ccttctcccg ttccaccagg atgcgcagcg ccgccgactg gacccggccg
    10501 gccgaaagac ccttcttgat gaggtcgccg accagcgggc tgagggtata tcccaccagc
    10561 cggtccagga cccgccgggc ctgctgggca ttaaccaggt ggaggtcaat ctcgcgcggg
    10621 gaattgaagg cgcgccggac cgcctccggg gtgatctcgt ggaaggtaac ccgcttgatc
    10681 tcttcggccg gacgcttgag aagctgggcg acgtgccagg cgatcgcctc gccctcgcga
    10741 tcctcgtcgg tggccaggaa gatgacggcg gccgaaccgg cctccttgcg cacctgggag
    10801 accaccttct gcttgcgggg catgacccgg taggtcggtt cgaactcctt ctcgacgttg
    10861 actcccagct cgtcggcggg caggtcccgg atgtggccca tggtggcgac cacccggtaa
    10921 tcgttcccca ggtacttgcc gatggtccgg cacttggccg gcgactctac gacgaccagc
    10981 gatattcctt ctttcatagt cggagttgtt gaagttgccc cttgaatgtc aaggcttaag
    11041 ggtgtctgat cttgtggcga taacagaata taacagagag accggctctt gtcaaatggc
    11101 aggaaaagat ggtagaataa tatgatacag tcgtttgccg ggcgggttta attccggaat
    11161 tgaaaactca agccgtagtt tacggcccca tcgtctagag gcccaggaca ggtggttctc
    11221 agccatcaga caggggcttg aaagccgctc tccggctttc aaggagagcc gtgaagtaga
    11281 gagccgcgat gcaaatcggc tcacacttca cggccatgaa gcgtgttcga agaactctgc
    11341 ttcacggcga cggttgtttc tgaacttttt tcagaagaac ctttaggaag attcttacac
    11401 cggcgccaaa tccaccggga tttgaacccc gggaattgaa aacttcagcc gtaattcacg
    11461 gccccatcgt ctagaggcct aggacaggtg gttctcagcc atcagacagg ggttcaaatc
    11521 cccttggggc tgcttccctg aagcgtttct ttggggattt gaacatccca cacttaaggt
    11581 ttagggtggc tcactttctc gccacccgac ccgcatctcg gccagcctcg aggagattat
    11641 cggctccttc cggtggtctt cagcatcgag ggtagcgcac ggatataaac ggctgcctcc
    11701 ctcatcatac ccagatcgga gggatggccg tctgaccccg tggcttctcc atcatctcca
    11761 tacatcccct tgtttggtag gaagaaaagc ccggtcatac cctgtttttt caactgctca
    11821 taaacggacc gccacgcccc tccttcgctg ggacggtcaa aacgatcggc accctggacc
    11881 agcaggatgg gagttgcggg acgaagcttc cgcaatctcg tcacaaatag cggcgcttgt
    11941 ttctcgggcg cataaggccc gttcgacttc atgttcgcgg cgcaatcaac aatgtaaatc
    12001 gacgcgtcga tttcccccat taaatccgcc agctccgtat cacccgtgcc gttggcggaa
    12061 aaccccaggt tgatgaccgg ccggtcgaga cggcgaccga cgatggccgt gtagaccatg
    12121 cccggacgcg aggcgcaggc cccctgggtg atcgaggtgc catagaagat aatgggcttg
    12181 gcccgggggg cgggtttcga gatggtccgc cccttgggaa tgccgatttc aacggatttc
    12241 acgcccgtgt ataaaggaag atagagcatc aattccttac tggcgccgac gctaaaaagt
    12301 gccgtgttgg tgatacccgt cggctccccg gttccgacaa acgtccattt tccggcagcc
    12361 tcccggcaat aaagatccac accgctgcaa gccgtcttcg gcatcgttga aagagaatac
    12421 agtccggtaa tgatccaacg gacctggata gtcggcgcat cggtgacaaa gcggatgcac
    12481 atgccggcgg tggcgtgact caacttccaa accgaatccc gcacggtacc cctggctttt
    12541 gcgggcaggc ggtcgtagaa ggattccgtc tccgtccagc ctctgccctc aacgtcaaac
    12601 cgggccccgt cgtaccagac ggtatcggtt tcgtccgcca ttttcacgga ggaatccggc
    12661 ggggtgggcg gcaccccgac ctcggctgaa gcgggcactt ctttcccggc gccgctggcc
    12721 gtccgctgtt ttgaagcgcc cattccggcg gtctgcagca acctgggcag gacctcgatg
    12781 aaaacagcgg cctgccgcat catgcccagg tcgctgggat gaacgccatc cacggtagct
    12841 tcgccgtcat ccccccacat tcccttgttc ggcaggaaga aaagcccggt cacaccctgt
    12901 tttttcaacc gctcataaat tgaacgcaaa gcccgccctt tgacggtgga acggttccag
    12961 caattcggat cttcgaccag taaaatgggg gttgcgggac gcgcttggcg taacttcatt
    13021 acaaatggtg ccgcgcgctg cataatcaac tccgcctcca tatccgcggt gcagtcaagg
    13081 acgtaaatcg acgggtcgag ctcccccagc aaatccgcca gctccggctc catcctgcct
    13141 ccgccggcaa agcccagatt gatgatcggc acatcgagac tccgaccgac gatggccgga
    13201 aaggccttac ccggacgcga ggcggatgcc ccctgggtga tcgaggtgcc ataaaaaagg
    13261 actggtttga cccgcgtgtc agccggaggg gcgggcctcg agataatccg gtccctggga
    13321 atgccgaact ccacggattt cataccgttg taagagggaa gataaagccg tatttccttg
    13381 ctgccgtcaa cattatagaa ggccgtctcg aaaccttccg tcttctttgg actctgtgcg
    13441 gaattcggtt ttccatgcgc aaagacagac acccatttcc cattcttatc acaacaatag
    13501 agatccatgc cgctggttgc cctggttgac atgtgcgaca tagcctcggg cttgcgcaga
    13561 agcttataac ggacctggat ctccggcgcg tcggtgacaa accggaagca catcccggcg
    13621 gtgtcgtggc ttagctccca aatggcggcc ggcacggtcg tcttggcctt tgagggcagg
    13681 cggtcgtaaa aagattccgt ctccgtccag cctctgccct caacgccaag ccgggccccg
    13741 tcataccaga cggtattgcc atccggatcg gccgtgccgt tcaggggaga gacggggtcg
    13801 gcctgaagca gacccagcca gcccgcaaga gcgaaaaaca ggaaggagac aattcgaatg
    13861 gattgggaac gcatcgaaac accttcaggt cttcagtttc agcaaccagc ggacaaaaaa
    13921 actgttttgt aacttatgat tgagcggttg gccggacaaa accggtagct tatacttgag
    13981 tatatcaaaa aagagccgaa atggaaaccc cggccgccag gttaattccc ggcggccgcg
    14041 gttttttatt tcccgctata ttccttcaat ccctcttaac ggcccattcc ttcttcggcg
    14101 ccgatcgatt caatcggatt ccaaacacgc aatttatcaa cccgacaatc aattccatcg
    14161 cgtgctatat catccttgca tttcattgca ttttaagctg gcctaattta taatcgaaac
    14221 agaggaaatt ccaaaacacc cttaatacaa aagggagggt ctgatgtgcg ataacagtcc
    14281 cgaagccggg gagaagaagg gcgaaaatcc tgacgacaag caaaaataca tacaactcgt
    14341 ctatcgatcc gccctggagg acatccgttt cttcaaaagc cagcagtggc tcgttaccta
    14401 ttatacacta ctggtttatt gggtacttgt tgctgctgcg aaatcaatta attatggggc
    14461 atggttgtca tgccttgttc ctgttgtttt agttatgtct tgtgcgctta tctgtcaact
    14521 tgagcattca ataaaagaac agagaaagat aaaggatgaa gcaatcgaga aaatcaagac
    14581 cgtttccgaa gaaataaaag atttatttcc aaatctccca acaacgtttt ctgaattgcc
    14641 tttatttata ttattatcga tgacgattct aggcgggtgc gtaacagttt ggattatcac
    14701 aaacctgccc ggcttctgcc aagcagccgg ttgccgccgc tgacagcctc accccaggta
    14761 ttctccgata tcggccgcgt agacccggcg gtatccctcg tgcgcggagt cgaaagaaat
    14821 aaggttcggc ggccgccccc agcggggatg caggtcgcac cgtatcgggc cggtgatttc
    14881 aggcggggag aaatactctc cggtggttat ttcccggtcc cggacggtat cgaaaagctt
    14941 gaggctgcgc tggccggcct gggggtaggt gtcgagcagg atgaatttcc ggtccggcga
    15001 gtagctgcag tggccgtcca ggaactggat gttttcgctt aagaaccggc cgtggccggt
    15061 ccgatcgtcg atcagccaga aaccgctttt cccaccgtac tccatccagg cgagcagctc
    15121 ccggccgtcc ttccagtcga aatgggagac cagctgggtc tccaccaggt cgtgcagacc
    15181 gctcccgtcc gggttggcag tgaagatccg cgtccgatgg cgctccttcg gcccggactt
    15241 ccagcggttg aggaagataa agcgccggcc gtccggggag aagagggtgt ggttcaccca
    15301 gccgaaagaa tcgcgcgtct ccggttccgg ccggagttcc agcaagccgg ccaaagaaat
    15361 gaccagccgg tggccgccgg tctccaggtc gaccaggtaa acgccgtcgg cctccggcca
    15421 tttttgatgc tcaaagggat ccgccactcc ctcgtaaccg taaccgggcc gccaccgggc
    15481 cagccgggcg taattgaggc tcagggccag ccggccgtca cggctgacgg cgcttaccgg
    15541 gcgcggcaat tcagaccggc ggccgctctt caggtcgtgg atgacgctga taaaatcgcc
    15601 gccgcggacg gagttgtaga ttatccggcg gtcgggcagc cactggagca tggccccctg
    15661 ctggaagttc caggcgggag tctcgtcgag gacctccacc cggccggtct tcgtctccag
    15721 caggcagatc cgggcccggt cgccggccgc cggcggccgg tcctgaaagt caacctcgtg
    15781 gaagaggtga tactccccgc tctcgtccca gccgcacttg tcgtagtagc cgaagaagca
    15841 gaaacgcccc tccgggctga tggccttgac cacgcttcgc ctcccctccg gacccggacg
    15901 gaccccggaa cttacagtgc cagtttttcg gggcggccgc tctcccggga gcggttggcc
    15961 gcctcgagga aggccatgat ctccagggtc tcctgcaggt caaccggcga tttcccggtc
    16021 ttgaagaagt cgaagatctt ctccaggatc agggcgtagg tgggcggttc ggcctgaacg
    16081 atgccgtgca cgagcccgcc atcggtcaga acggagcagc cgaactgctg gggaacgttg
    16141 tcgacccggt agccgtaaag gacgccggtc cggccgtcct cccagaggcc ggtgaccgcg
    16201 tcggccgcct cgcccttctg gacgaagacc tggcggcagc cgctgcccag tttggagaag
    16261 agcacctcgg cgctgtgcac gccgtaccag tagaggccgg ggtaatcggg caggatcttc
    16321 atgttgccga aggcgatgca gccgaatacc cggcgcccct gctcgatgcc ggccaccccg
    16381 gccgcgtagc gcatggccga ggcggagaag atcggggccc ggtgctgctt cgaaagggcg
    16441 atgatctcgt gggcgtcggc ggccgaacag gtgaagggct tgtcgatgaa gaccggcttg
    16501 ccgaagggcg cgatcacccg gaactgctcc aggtgctgac ggccgtcgac gctctccagg
    16561 aagagggcgt cgacctgctt catgaccgac tcgatcgaat cgcagatctc gacgccgaac
    16621 tcgtccttga actggctggt gaactgggga acccggtcgc ggctcatgga gaaggcctgg
    16681 ctgccgccgg ggaaggcggc ggtgacccgg ccgccgggaa cgtggaaggg cgcccccgga
    16741 aagttgagca gcttggtgaa ggaaacggcg tgcgaggtat ccaggccgac catgccaatc
    16801 ttcagttccg acatttctgc ctccattttg ggaaagttcg agcgccgggg ctcccggacg
    16861 cggagtcgtt ccgtaaaatc ctattttaat atttattcga gccgcacgca aaaaccgggc
    16921 ggacttacgg ccgggccggc gcgccccttt gcggccggct tgcattttca ccgccacctc
    16981 ggttataatt tttcattgag ccgttggaac gcaagaccct ggggccgtgg tgtagcggtt
    17041 aacattcctg actgtcgatc aggagagcgc gagttcgaat ctcgtcggcc ccgcctacgc
    17101 tcaaaaagcg agctttggcg gacatgcccg ccaacagttg aaaaacagcg ccccctgaag
    17161 agatttgaac tccaaagggt gtttgttgta ttcaacatct ttatcgtttc atattgttgc
    17221 gatgccggat tcccgcctgt tgcgggcggg aacgacaagc gaaaaacgtc gcatctcatc
    17281 gcgatggcca ttgattaaaa aggtctccaa cccgggcccg cccacgattg cgtaatcggt
    17341 tgccgcgacc ggagaacggc ccgtaaccac gaaaccggag cctgtcatgg aagaaagaat
    17401 tctcctctcc gagaaggaaa ttccgcagca gtggtataac ctcctgaccg atctgaaaac
    17461 ccctctggcg ccgccccgcc acccggccac cggcgaaccg ctgaaaccgg aggacctcta
    17521 cccgatcttt cccaaaagcc tggtggccca ggaggtctcg agcgaacgct ggatcgaaat
    17581 tcccgatccg atcctggaga tctaccggct ctggagacca actccgctct gccgggcccg
    17641 ccggctggag aaggccctgg gcaccaaggc ccagatctgg tacaagtacg agggcgccag
    17701 cccggccggc agccacaagc ccaacaccgc aatcgcccag gcttactaca ataagcagga
    17761 gggcgtcacc cgcctctcga ctgaaaccgg agccggccag tggggcagcg ccctgtcgct
    17821 ggcctgccag ctgctgggcc tcaagtgcac ggtctacatg gtcaagggca gctacgagca
    17881 gaaaccctac cggcgcatca tgatggagac ctggggagcc gaggtcttcg cctcgcccag
    17941 cgacaagacc gaaagcggcc ggcgcatcca ggtggagatg cccggcacgc ccggcagcct
    18001 gggcatcgcc atttcggagg cggtcgagga tgccgccacc cacgaggaca ccaagtattc
    18061 gctgggcagc gtgcttaacc acgtctgcct gcaccagacg gtggtcggcc tggaaaccaa
    18121 gaaacagttc aagctggccg gtttgaagcc ggacatcctg atcggctgcg tcggcggcgg
    18181 cagcaacttc gccgggctgg tctttccctt tgtgccggag aagctggccg gacaggagat
    18241 gaggttcatc gcggtcgaat cggccgcctg cccctcgctg acccgcggcc agttcgccta
    18301 cgatttcggc gataccgccg gcctgacgcc gctcctgaaa atgttctcca tcggccaccg
    18361 cttcgtgccg gcgccggttc acgccggcgg actccgttat cacggcatgg cgccgatggt
    18421 ctcccacctg atggccgaaa aactgatcga ggcccgggct taccagcaga aggaggtctt
    18481 cgaggcggcc ctgctcttcg cccggaccga gggcatcatc ccggcgccgg agaccaacca
    18541 cgccctgaag gcggccatcg acgtggcccg ggaatgccgg gaagaaaagg tgatcctgat
    18601 caacttctcc ggccacggcc acttcgacct gagcgcctac gaggcctttc tgaccggccg
    18661 gatgaccgac tcgacggtct ccgatgaaac gctgaacaaa agcatgggag atttaaagaa
    18721 aatctaagtg gcatcaaaaa ggaggcgcat ggaacggatt ctgcttaaga aatgggtggt
    18781 gttgctggta atggcggtgg cggtgaccat ttcgctgcgg ccggccagcc tgcaggcggc
    18841 cgtggtaccg gcccagaccg cgaccgcatc cggaaacgtg aaccagatcc tggacaatcc
    18901 ggaggtcatg tcccggctgg ccgcgctcgg atatacccgc gccgaagtcc gcgaaacgct
    18961 ggtttcaatg acgccggccc agcgggcgga cctggaggat cgggtcagcc tgctctcccg
    19021 gggcggcaac ggactgggcg tgatagtggt aatcctggtc atcgtggtct ttatcgcact
    19081 gcttctggaa gccagcggcc accgtttgag cattgagacc aaagccggca attagaagtt
    19141 ggccctcgga agggcgcgcg ctactggcgg ccttcctgtt gatggccgcc ggaccgctgc
    19201 cagccggacc ggtccggctg gaggttccct attcccgggg accgaaaggc ggcaacgcct
    19261 gcggaccggc cgttc
//
LOCUS       DAOQYN010000008        19215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_3217_length_19215_cov_4.340919, whole genome shotgun sequence.
ACCESSION   DAOQYN010000008 DAOQYN010000000
VERSION     DAOQYN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3217_length_19215_cov_4.340919"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PKN80_00750"
     CDS             <1..1292
                     /locus_tag="PKN80_00750"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNQ34578.1"
                     /translation="LRALESARKPRLHLFLATSEIHRRYKLGKAKSEIVRLAVNAVKQ
                     ARQTLDNIEFSPEDASRTEPEFLLEVAQAVAAAGARTLNIPDTVGYAVPEEFGALIAF
                     LRQRLGREIAISVHCHNDLGLSVANSLAAIAAGADQVEATVNGIGERAGNASLEEIAV
                     TLHIRRDYWKCRTNIKLSELYRASRLVSIQTGLVVQRNKAVVGANAFKHEAGIHQDGI
                     LKDRRTYEIIEPSLIGVPESELVLGKHSGRHALNARLVKLGYRFREDELVRIFERFKD
                     LADKKKEVTDADLMALMEETIAPLSETYTLEYLSTVAGTNTIPMATVRLKKGGETSQA
                     ISNGDGPVDAACKAIDQITGLKARLSNYRINAVTGGRDALGEVTLLINVEGREIPGRG
                     VSTDIVEASVKAYLNAVNRYFQHQAGSAGEETETPSL"
     gene            1342..2610
                     /gene="leuC"
                     /locus_tag="PKN80_00755"
     CDS             1342..2610
                     /gene="leuC"
                     /locus_tag="PKN80_00755"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNQ34579.1"
                     /translation="MTLSEQILSARSGQERLRPGQFILVDVDLGFANDLTAPPAIKEF
                     RKYGSQVFDPGRVALVPDHFTPCKDIESAELVKLMRNFAHEQGIERFYEIGRVGVEHV
                     LLPDAGLITPGMVVIGADSHTCTHGALGAFATGVGSTDFAFFLLTGKAWFRVPESIRV
                     LLSGRLQSWVGGKDIVLYLISRLGVDGARYQTLEFAGETLAQLSMDDRFTICNMAIEA
                     GAKNGIIPPDNVTMEYLYQVNPDWVNRHPGFALDPGKKAVYSREMKLDVSKLEPYVAA
                     PDLPSNGHPVSELKEVKLDQVVIGSCTNGRLSDLKTAASVIKGRKVSSGTRLIVIPAT
                     QDVFRQAMAAGYLDTFVESGAVVTTPTCGPCLGGHTGVLAEGEVGLSTTNRNFVGRMG
                     HPKSRLYLSSPAVAAASAVTGRITHPSEVV"
     gene            2610..3095
                     /locus_tag="PKN80_00760"
     CDS             2610..3095
                     /locus_tag="PKN80_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNQ34580.1"
                     /translation="MKIKGNCWKFGDNINTDLIIPARYMTAYSAEELGRHCMEDADPE
                     FPTKVRPGDIIVAGENFGCGSSREHAVLALKGAGVALVIARSFARIFFRNAINTGLPI
                     IESPEAASAIEAGDDIEVDLSAGRIDNQTTGEQYTIAEFPEFLTQIIEKGGLLPWAKT
                     L"
     gene            3077..4162
                     /locus_tag="PKN80_00765"
     CDS             3077..4162
                     /locus_tag="PKN80_00765"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNQ34581.1"
                     /translation="MGKDAVKTYRIGVIPGDGVGPEIIREGLKVLEAARSRYAMKFDL
                     VHYDFGGERYLKTGEILPDSAIGEFRGLDAIFLGAVGHPDVKPGILEHGLLLKLRFAL
                     DQYINLRPVKLYPGVWTPLKDKGPEQIDYVVVRENTEGAYCGIGGSFKKGTAEEIAVQ
                     QDINTRKGVERCIRYAYRLCQRRNQRKKLTCCDKANVLTYAGDLWRRTFEEVGREYPE
                     ISRDYAFVDATTMWMVKNPEWFDVIVTNNMFGDIITDLAAVTQGGLGIAAGGNINPKG
                     VSMFEPIHGSAPKYTGKNVICPLATIASGAMMLDSLGETEAAKAIDAAIWKATGEGHV
                     KSLEAGKMGLGTAEVGDLIARFVSEAR"
     gene            4202..4272
                     /locus_tag="PKN80_00770"
     tRNA            4202..4272
                     /locus_tag="PKN80_00770"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4234..4236,aa:Gln,seq:ctg)
     gene            4638..5075
                     /locus_tag="PKN80_00775"
     CDS             4638..5075
                     /locus_tag="PKN80_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ34582.1"
                     /translation="MRLFRRKAEEVEEPVNIASVGKLKILTADDEEDIRVLLKKMLEA
                     EGHQVISAVDGEDALQKLKDEKPDLLILDKKMPKLNGLKVLEKVRQDPSTRNLPVIML
                     TVDASDKDVYQGYMQGADVYLAKPFKLNNILIGIVMALKKARK"
     gene            5223..5804
                     /locus_tag="PKN80_00780"
     CDS             5223..5804
                     /locus_tag="PKN80_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018752316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ34583.1"
                     /translation="MAFTLEIGERAPDFSLPATDGRRYTLADFDGSALLVVFFTCNHC
                     PYVTGSDEVTRATAEKYRERGVTFIGINSNSEQTHPTDDFDHMVERMRVNRFPWIYAR
                     DRSQETARAYGALRTPHFYVFDRERRLVYTGRGVDSPRDTSKSTVNDLDRALSELLAG
                     KPISVPKTNPIGCNVKWEGRDAHWMPPEACDLV"
     gene            complement(5931..7217)
                     /locus_tag="PKN80_00785"
     CDS             complement(5931..7217)
                     /locus_tag="PKN80_00785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNQ34584.1"
                     /translation="MKRMIIRAGLLLLAATALLPGFPRAQEAPLLPRNARLAIAGDSI
                     TEQKIYSRYIETYLLACAGRRDISVFQFGWSGETAPEFAARMANDLGAFFKPTAMTTC
                     YGMNDGRYRPYEETIGKNYEAGMRAIIKKARELGVTTVIVGSPGAVDTNFFQKSGTGA
                     AEYNANLRQLGNLAREIAAEYKASFADVHAPLIEAMARAKAVLGENYDVCGRDGVHPG
                     SNGHLVMAYAFLKALGCDGNIGEISVNRQGGTAVSSGHRVLSSTGGEIKLESERYPFC
                     YDPDPRSSSSNRSILPFLPFNQELNRFILKVPELASARARVAWGNESKEFTAGQLASG
                     INLAAEFDRTPFDGAFQKVQQSVAVQQYFQTRAIRSLVTNLRAFADDEISGGAEWQNA
                     VNVLIQKLKARDDQFQAAVRAAIIPVQHTIVITPLP"
     gene            complement(7253..8218)
                     /locus_tag="PKN80_00790"
     CDS             complement(7253..8218)
                     /locus_tag="PKN80_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ34585.1"
                     /translation="MEVKEIKAEGMDVAGFIKDKTDEISSLVKDGLAITALSGGVDSS
                     VVTVLGHQALGNRLLTYFIENGLMRQDEARNVAEIFGKFGIPVKIVDARQEFFEALKG
                     LTDPEEKREAITQTFYKKVFGKLVVESGARHLLQGTILTDIDETVAGIKRQHNVFEQL
                     GIDPQKAFGYRIIEPLRQIRKDGVRKVAQTLGLPESIYKRMPFPGPALAARIIGEVTP
                     EKIELVRKATVITEAELAGTEAFQYLAILHQDRVTGMRGGRRDFGLQIEIRCWKSLDA
                     RTAAPVRLPYETLERLAGRITGEVPGVVSVTYNIATKPPSTMEAI"
     gene            complement(8373..10697)
                     /locus_tag="PKN80_00795"
     CDS             complement(8373..10697)
                     /locus_tag="PKN80_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34586.1"
                     /translation="MNPGSRRLGIFAGLLLTLTGAAGAWGQTANTDPSAGYVYPAGGR
                     RGATVQVTVGGQNLRGLSAAYISGDGVSAANLRYIPPLTSLQKDALRRRITAIREKRR
                     AEAAARRGLPVPPVKQPEAETEPAKPVELPDHPLLRDLDNLSNEDLKKAYEIFLDPGR
                     KQPVKRSIQELAILDLSIAEDAPAGDRELRLLAAGGLTNPLRFQVGPAPEILETEPND
                     PDAPAGTAVELPAVFNGQIMPGDSDCFTFKARRGQKLVLRAQARSLIPYLADAVPGWF
                     QAAIAIYDPDGREAAFADHYRFDPDPVILYEVPRDGEYRVEIRDSIYRGREDFVYRLY
                     AGESPFVTRVFPLGGRAALPTAAALSGWNLPESRLPLDTNFEPGTIRTVSFINNGQIS
                     NPVLYSVDNLPESIESESGSSAAAQKVRLPQIINGRIGKPGDTDRFQFEGRSGFEFVA
                     EVMARRLGSPLDSRLRLMDASGRVLAWNDDREDRGSGLNTHHADSYLSFKLPRDGTYQ
                     VEIADSQDHGGEEYAYRLRIGPRIPGFELRVTPSSLAIPAGCVTPINVHALRRDGFDG
                     EIEMLLKEAPAGWILNGGLIPAGRESARMTLSAPREPSPEPFFLQLEGRALVNGQPVS
                     RPAVPAEDMMQAFAYRHLVPSETLLALVPQARLRRPAFELAEPGPVNLQAGGMVRVSA
                     RVPPRLHLASCSLSLVDPPRGISLGEVNLSSGLLTFMLKSGANEVQPGLVDNLIVEAF
                     TEWPSGPPDEKGNRPKRRVSLGMLPAISIRVTPP"
     gene            complement(10694..12079)
                     /locus_tag="PKN80_00800"
     CDS             complement(10694..12079)
                     /locus_tag="PKN80_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNQ34587.1"
                     /translation="MPPLKIERPGKEFRSGRPARAGAASRPASFLRRSGAALLILAAL
                     SLAAGAEEPAGPSAAGGRARSVIQIWLWGGPSHLDTFDPKPEAGYDYCGPFDKTIQTN
                     IPGIQINELLPRLAKIADKYSIIRSLTHGIDGHETAAYITQTGHKPGEALSYPSVGAV
                     VALFKGYEHGYRGQLPPYIILTTSQGRFSEVGFLGSKYKPLVTGGDPNGKTFAVEGLS
                     APGISSERQLGRWDLLKKLDTLGRTMPADPYFASFDRSLNEAHELITGDAARVFDLTT
                     EKDEVRDRYGRTRFGQSCLVARRLVESGVPYVTINYGGWDTHKQHFQSMRQKLPELDN
                     GLSALLEDLAGRGLLESTVVWCCGEFGRTPRILWEAPWNGGRGHYGKCFSVLVAGGGF
                     KGGRVVGTSDARGETVKERPVTPQDLIASLYTLLGIDPDGRLPDSQGRNLPLLPPPTP
                     GTSRNLLREIM"
     gene            complement(12067..13650)
                     /locus_tag="PKN80_00805"
     CDS             complement(12067..13650)
                     /locus_tag="PKN80_00805"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNQ34588.1"
                     /translation="MKKTLLLGLAAGLLLAPASSGADTVRRPAAPFEGAGWTAPANFI
                     DTCVLAGLGKRGIKPAPLCSDEIFVRRVYLDVIGTLPEPAEVTAFLEDPRPDKRSALI
                     DTLLARPEFADYWSLKWCDILRVKAEFPINLWPNAVQAYHRWIRDSLRENLPYDRFAR
                     ALLTSSGSNFRVPPVNFYRAVQGRDAPSIARVVALTFMGTRLESWPAERRAGLEACFS
                     RIAYKKTAEWKEEIVQLDPAPAAVIETVMADGTRLKIPPEQDPRRVFADWLLAPGNPW
                     FTRSIVNRAWSWFMSYGIIHEPDDLRPDNPPVNPELLAGLEKYLVDNKYDLKLLFKAI
                     LNSNTYQQSSIPDGNNPESARFFACYPVRQLEAEVLIDALNWIAGGGGEEYSSPIPEP
                     FTFTPRRQRSILLSDGSITSPFLEMFGRPGRDTGLESERSRQPSAAQRMCLMNSSEIQ
                     RKITMSSRLQKMLASVKWNRELIIEQTYLIILSRRPTREEAEIAAAYFQANTSLGYKA
                     ANDDLAWTLINSKEFLCRH"
     gene            complement(13647..14738)
                     /locus_tag="PKN80_00810"
     CDS             complement(13647..14738)
                     /locus_tag="PKN80_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNQ34589.1"
                     /translation="MIERVAGSGLHSIKLDLGETSHYFITVRPQGGKESPDAWFARAV
                     SPVQSLNASILGQDLFGTITANSSVPTVEPEWPVTRLRGTGNGNGLAGTQLQAVTGAG
                     VRPIRLEGRTVGYVYDDGHSRCCRLGDVRAADTSLSREKQAQAVFESIERALEAAGLS
                     FTDLVRTWFYLDDILAWYGDFNRVRTGFFAARRVFDRLVPASTGIGGGNAAGAALVAN
                     ALAVRPLDRSIRKSAVASPLQCPALRYGSSFSRAVEISGSGWRQLLVSGTAAIHPDGK
                     SAHPGNFEKQALLTLDVVKAILESRRMAWRDVTRAITYLKNGEDAPAFERMLANLNFP
                     DLPMLVIENTICRDDLLFEIEVDAAAPGK"
     gene            15013..16038
                     /gene="pyk"
                     /locus_tag="PKN80_00815"
     CDS             15013..16038
                     /gene="pyk"
                     /locus_tag="PKN80_00815"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNQ34590.1"
                     /translation="MVKTRIICTLGPASSSETVLRKMMRAGMDVGRLNFSHSRPRELL
                     PGINLIRSLNRKYRRRVRLLGDLQGHRIRIGGLAAPLELKRRQVLYLVRAGAAVGAGR
                     IPFDYRGPLESIKTGQPIFLDDGNLVLEVTAVFKNALKTRVVVGGRLKGHKGVNIPDA
                     KLDFGPINPRDVEDILFCAENQLDYLAQSFVRSAGDIMEVRKILGRNSRCRVIAKIEN
                     REGVRNIDEIIEASDGIMIARGDLGVSLPIQEIPILQKRLIRKCNQAGKFVITATQML
                     ESMTENPRPTRAEVTDVANSIIDGTDYVMLSAESAVGKYPVETVAMMNGIIKFTEGYL
                     KAGKPGR"
     gene            complement(16130..17569)
                     /gene="gatB"
                     /locus_tag="PKN80_00820"
     CDS             complement(16130..17569)
                     /gene="gatB"
                     /locus_tag="PKN80_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNQ34591.1"
                     /translation="MTTKYEAVVGLEIHVQLNAATKMFCDCPNRFGDEPNQNTCPICL
                     WFPGASAQLSREVVEKAVGVAMTLNCTINQRSRFDQKVYYYPDLPKGFQLSQFHRPLA
                     GNGWVEITGEDGALRRLRIRTLHLEEDVAKLVHETEGKTRVSLVDFNRAGCPLVEIVT
                     EPDMRSPYEAMEFVNAIRTLVPGAGGAECSLERGTMRVDANISLRPAGSQELNVKVEV
                     KNMNSVRGIGDAIAYEIKRQQELIQKGEAIILHTRLWDPKSGRTLPMREKFSGPCVPD
                     PSVPEVVISDQWLAEIKNGLPEIPARKQLRFVEEYGLTETEAAGLTVSPESADYSEAV
                     IRAGIPARLAAQWIAGRLLPAAARRGLTLAETKITPERLAGLLALVENGKINPNAGRQ
                     LLELLFEDGRTPAEIAREQGLDQAADSGELEKILARVLAENPRAVEDFRKGRSQSLGF
                     LTGQAVRLSGGKADPRLIRQKLEEKLRSE"
     gene            complement(17566..>18505)
                     /locus_tag="PKN80_00825"
     CDS             complement(17566..>18505)
                     /locus_tag="PKN80_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNQ34592.1"
                     /translation="LGDYLAFMADLAGEPRYAAAAGGRFGFKPSHGTVSRAGLIGLMP
                     SLEAVGLLGNHPADVAAAMAEIAGFDGKDFSLEAEPLAGFPVRPPKTESKPVLGRPAG
                     CEARLSPAAREIFSAGLKKLEGAGFPVREVPWPGDETFRDLHRVIGSVEASSSCGKFD
                     GVRYGLRADSANNWNDMYLKTRAASFSTFLKTLLFQGAFFQFESYGTFERAARIRARL
                     IREIGQLFETVDLIISPVRPETPAAESAREVDQTYAAFSLTLPANVTGCPALAFSLSG
                     GREASDPGLQVMGGFRSDAFLLACGKRLFETLTGGA"
     assembly_gap    18506..18515
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18516..18773)
                     /locus_tag="PKN80_00830"
     CDS             complement(<18516..18773)
                     /locus_tag="PKN80_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNQ34593.1"
                     /translation="MKISESENPDRLRDKKLIAPANFSVRDQVSDAGSPALEKFTALE
                     NATVVQRSLEAGAVLAGRAATAEFGFGLNGDACLKNFTSQAA"
     gene            complement(18770..>19215)
                     /locus_tag="PKN80_00835"
     CDS             complement(18770..>19215)
                     /locus_tag="PKN80_00835"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030933.1"
                     /note="contains both tRNA synthetase and amidotransferase
                     domains; catalyzes a two-step reaction, first charging an
                     aspartate molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; contains discriminating
                     and non-discriminating subtypes; allows the formation of
                     correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through
                     the transamidation of misacylated Asp-tRNA(Asn) or
                     Glu-tRNA(Gln) in organisms which lack either or both of
                     asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction
                     takes place in the presence of glutamine and ATP through
                     an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HNQ34594.1"
                     /translation="MILGTASIRDVIAFPKNRSAVCPLTQAPAPVTPEQLAELHLAGE
                     GELKISGRREEDPVKRLAWVSRLGYEESERNELKKAVEAAADLAGRIEKLPADAEPVY
                     SPSRPGNRMRPGRTASKYPGVEKKDLFRNAPAVKNGHFKVANILE"
BASE COUNT         3476 a         5834 c         6225 g         3670 t
ORIGIN      
        1 ccctgcgggc cctggaatcg gccagaaaac cgcgcctgca tcttttcctg gccacctccg
       61 agatacaccg ccgctacaag ctgggcaagg cgaaatcgga gatcgtgcgc ctggcggtca
      121 acgcggtgaa acaggccagg cagacgctcg acaacatcga gttttcaccc gaggacgcct
      181 cgcggaccga gccggaattt ctgctggaag tagcccaggc ggtggccgcc gccggcgccc
      241 ggacccttaa cattcccgac accgtcggtt acgccgtgcc ggaggaattc ggtgccctga
      301 ttgcctttct gcgccagcgc ctcggccggg agattgcgat cagcgtccac tgccacaacg
      361 atctcggcct ctcggtggcc aacagcttgg cggccatcgc cgccggagcc gaccaggtcg
      421 aggcgaccgt caacggcatc ggtgaacggg cgggcaacgc ctcgctcgag gagatcgccg
      481 tcaccctgca catccgacgc gattactgga agtgccggac caacatcaag ctttcggaac
      541 tctaccgggc cagccgcctg gtaagcatcc agaccggcct ggtcgtccag cgcaacaagg
      601 cggtggtggg tgccaacgcc ttcaagcacg aggccggcat ccaccaggat ggcatcctca
      661 aggaccggcg cacctacgag attatcgagc cgtccctgat cggggtcccg gagagcgaac
      721 tggtgctcgg taagcactcg ggccgccacg ccctgaacgc ccggctcgtc aagctgggct
      781 accggttcag ggaggatgaa ctggtccgga tattcgaacg attcaaggac ctggccgaca
      841 agaagaaaga ggttaccgac gccgatctga tggctctgat ggaggagaca attgcgcccc
      901 tgagcgagac ctacacgctg gaatatcttt cgaccgtggc cgggaccaac accatcccga
      961 tggctacggt tcggctcaag aagggaggcg aaaccagtca ggccatctcc aacggggacg
     1021 gtccggtgga tgccgcctgc aaggcgatcg accagatcac cggcctgaag gcccggctca
     1081 gcaactaccg gatcaacgcc gtgaccggcg gccgggatgc gctgggagag gtgaccctgc
     1141 tgatcaacgt tgagggccgg gagatcccgg gccggggcgt gagcaccgat atcgtcgagg
     1201 caagcgtcaa ggcctatctg aacgcggtca accgctactt tcagcaccag gccgggtcgg
     1261 ccggggaaga gacggaaacc ccctcccttt gacccgtttc ctgcgttcgc aggttctcgc
     1321 gaaaacatcc agagagaaaa aatgacacta tcggaacaga ttctgtccgc ccggagcggc
     1381 caggaacgcc ttcgtcccgg gcagttcatc ctggtcgatg tcgatctcgg ctttgccaac
     1441 gatctgaccg ccccgccggc cattaaggaa ttccggaagt acggcagcca ggtcttcgat
     1501 cccggtcggg tggccctggt tccggatcac ttcacgccct gcaaggacat cgagagcgcc
     1561 gaactggtca agctgatgcg caacttcgcc catgagcagg gtatcgagcg tttttacgag
     1621 atcggccggg tgggagtcga gcatgtcctg cttcccgatg cgggcctgat cactcccggc
     1681 atggtggtca tcggggccga cagccatacc tgtacccacg gtgcgctggg ggctttcgcc
     1741 accggcgtcg gcagcaccga tttcgccttc ttcctgctga ccggcaaggc ctggttccgg
     1801 gtgccggaga gcatccgggt tctcctgtcc ggccgcctgc agtcctgggt gggaggcaag
     1861 gacatcgtcc tttacctcat ctccaggctt ggcgtcgacg gggcccgcta ccagaccctc
     1921 gagtttgccg gggagacgct tgcccagctt tcgatggacg accggttcac catctgcaac
     1981 atggccatcg aggccggggc caagaatggc atcataccgc cggataacgt gacgatggaa
     2041 tacctttacc aggtcaatcc cgactgggtc aaccgccatc cgggtttcgc gctggacccg
     2101 ggtaaaaagg ccgtttacag ccgggaaatg aaactggacg tctcgaaact cgaaccctac
     2161 gtggccgccc cggaccttcc ctccaacgga cacccggtct ccgagctgaa ggaggtcaag
     2221 ctggaccagg tggtgatcgg ttcctgcact aacggccgcc tctcggacct gaagacggcc
     2281 gcctcggtaa tcaagggacg caaggtttct tccggcaccc ggctgatcgt gatcccggcc
     2341 acccaggacg tcttccggca ggccatggcc gccggctacc tggatacctt cgtcgaatcg
     2401 ggcgcggtgg ttaccacgcc cacctgcggt ccctgcctgg gcggccacac cggcgtcctg
     2461 gccgaggggg aggtgggtct ttcaaccacc aaccggaact tcgtaggccg gatgggccat
     2521 cccaagagcc ggctttacct ttcttcaccg gcggtggccg ccgccagcgc cgtcaccggg
     2581 cgcataaccc atccttccga ggtggtctga tgaagataaa ggggaattgc tggaagttcg
     2641 gcgacaacat caacaccgac ctgatcatcc cggcccggta catgaccgcc tactcggccg
     2701 aggaacttgg ccgccactgc atggaggatg ccgatcccga gtttccgacc aaggtccggc
     2761 ccggtgacat catcgtggcc ggtgaaaact tcggctgcgg ctccagccgt gagcacgcgg
     2821 ttctggcttt gaagggggcc ggggtggccc tggtcatcgc ccgctccttc gcccggatct
     2881 tcttccggaa cgccatcaat accggcctgc cgattatcga gagcccggag gcggcctcgg
     2941 ccatcgaagc gggtgacgac atcgaggtcg acctgagtgc cggccggatc gataaccaga
     3001 ctaccggcga acagtacacc atcgccgaat ttccggaatt cctgacccag atcatcgaaa
     3061 aaggaggcct gctcccatgg gcaaagacgc tgtgaagacc taccgcattg gtgtcatccc
     3121 cggcgacggg gtcggtccgg agatcatccg ggaaggcttg aaagttctgg aggcggcccg
     3181 ttccaggtac gccatgaaat ttgacctggt ccactacgat ttcggcggcg aacgctacct
     3241 gaaaaccggc gaaatcttgc ccgacagcgc catcggggag ttccgcggcc tggatgccat
     3301 cttcctgggc gcggtcggcc atcccgatgt caagccgggc atcctggagc acggcctgct
     3361 gctcaagctg cggttcgctc tggaccagta catcaacctg cgtccggtca agctctaccc
     3421 gggcgtctgg actccgctca aggacaaggg tccggagcag atcgactacg tggtggtgcg
     3481 cgagaatacc gagggtgcct actgcggcat cggcggcagc ttcaagaagg gcaccgccga
     3541 ggagatcgcc gtccagcagg acatcaacac ccgcaaggga gtcgaacgct gcatccggta
     3601 tgcctatcgc ctctgccagc gccgcaacca gcgcaagaag ctgacctgct gcgacaaggc
     3661 caacgtgctt acctacgccg gcgacctctg gcggcgcacc ttcgaggagg tgggccggga
     3721 gtacccggag atcagccgcg attatgcctt tgttgacgcc accaccatgt ggatggtcaa
     3781 gaacccggag tggttcgacg tcatcgtgac caacaacatg ttcggcgata tcatcaccga
     3841 tctggccgcc gtgacccagg gcggcctggg catcgccgcc ggcggcaaca tcaaccccaa
     3901 gggcgtctcg atgtttgaac ccatccacgg ctcggccccc aagtacaccg gcaagaatgt
     3961 catctgcccg ctggcgacca tcgcctctgg agccatgatg ctggacagcc tgggcgagac
     4021 cgaagccgcc aaggccatcg acgcggccat ctggaaggcg accggtgagg ggcatgttaa
     4081 gagtttggag gccggcaaga tgggcctggg caccgctgag gtcggcgacc tgatcgcccg
     4141 gttcgtaagc gaagcgcgct gattttggtg cggatgtggt aaaatattgg gtctgattta
     4201 ttgggagatc gtctaatggt aggactgcag actctggatc tgcctatggt ggttcgaatc
     4261 cacctctccc agcagttcga ctcgcttcgt tcgctcaccg ccggctaata ctcgactcgt
     4321 ttcgctcgct caccgcaagt tcctgtttga ttcggtaagc gcataacctg aagtcgaata
     4381 atcccgggcg agtcgaaaag ggaggctaag ctccctgaca aaagaggtgg attcgaagcg
     4441 ctttccatcc cccgaccgtt cctgaccgac ccgttcgatc cgaaagtgcc tttctctccg
     4501 taatggccgc ctcgttctct taccggcgtt ttttctccgg gcaaggcgtt ccggggcagg
     4561 ttgacgtctt taagtttgca tgttaggata acattaaacg ggaaattatt attaacgccg
     4621 atagtgggga ggatgacatg cgattattca ggcggaaggc cgaggaggtt gaggaacccg
     4681 taaacattgc gtcggttggc aagctgaaaa ttctgacggc cgacgacgag gaggatatcc
     4741 gggtcctgct caagaaaatg cttgaggcgg aaggacacca ggtgatcagc gccgttgacg
     4801 gcgaggatgc cctccagaag cttaaggatg aaaaacccga tcttttgata ctcgacaaga
     4861 agatgccgaa gctgaacggc ctcaaggtgc tcgagaaggt caggcaggat ccttccaccc
     4921 ggaacctgcc ggtgatcatg ctcaccgtcg acgcttcgga caaggatgtt taccagggat
     4981 acatgcaggg cgccgacgtt tacctggcca agccgttcaa acttaacaac atcctgatcg
     5041 ggatcgtcat ggccttaaaa aaagcccgga aatgaacccg gctcttgttg ttttccgatg
     5101 gcgatggcca tgatcttgaa gaaggcccgg aaacagtttc cgggcctttt ttttgagtgg
     5161 tttgccgtct ttgcagtggg aagaaactgc gcggttatcg taggttttcc ggggaggaga
     5221 gaatggcctt tacgctggag ataggagaac gggcgccgga cttcagcctg ccggcgaccg
     5281 acggccggag atacaccctg gccgatttcg acgggtccgc gctgctggtg gtcttcttca
     5341 cctgcaacca ctgcccttac gtgaccggct ccgacgaggt gacccgggcc acggccgaaa
     5401 aataccggga gcggggcgtc acctttatcg gtatcaattc caacagcgag cagacccatc
     5461 ctaccgatga tttcgaccac atggtggagc gtatgcgggt taaccgcttc ccctggatct
     5521 acgcgcggga ccggtcccag gagacggccc gggcctacgg cgcgcttcgg acgccccact
     5581 tttatgtttt cgaccgggaa cggcggcttg tttataccgg ccgcggcgtg gacagtccgc
     5641 gcgacacctc gaagagcacc gtcaacgacc tcgatcgagc cctctccgaa ctcctggccg
     5701 ggaaaccgat cagcgttccg aagaccaacc ccatcggctg caacgtaaag tgggaaggcc
     5761 gggatgccca ctggatgccg cccgaggcct gcgacctggt ctgattagag caggaatcgt
     5821 tttgtccctg gacgtttttt ctgaaccgtt ccccaagggg aggcgcggtc agggcggttg
     5881 gataagacca ctgggcggct gcctcgcggg cagccggttt tttattgcga tcagggaagc
     5941 ggtgtgatta ctatggtgtg ctggacggga attatcgccg cgcgaaccgc cgcctggaac
     6001 tgatcatccc ttgccttcag cttctgaatc aacacgttga ccgcgttttg ccattcggcc
     6061 ccgccgctga tttcgtcatc cgcgaaagcg cgcagattgg ttaccaggga tctgatggcc
     6121 ctggtctgga aatactgttg gacggccact gactgctgga ctttctgaaa ggccccgtcg
     6181 aaaggggtcc ggtcaaattc ggcggcaagg ttgattcccg aggcaagctg gccggcggta
     6241 aattccttgc tttcgttgcc ccaggcaact ctggcccggg ccgaggcgag ctccggcacc
     6301 tttagaatga accggttcaa ctcctggttg aacgggagaa agggaagaat gctgcggttg
     6361 ctcgacgagg agcgcgggtc cggatcgtaa cagaaagggt agcgttcgct ttccagcttg
     6421 atttcgccgc cggttgagct cagcacccgg tgtccggacg aaactgccgt tccgccttgc
     6481 ctgttcacgg aaatttcgcc gatattcccg tcgcagccca gcgccttgag gaaagcgtag
     6541 gccatgacca ggtgcccgtt tgaacccggg tgaaccccgt cccggccgca aacgtcgtaa
     6601 ttctctccca ggacggcctt cgcccgggcc atggcttcga tcaggggcgc gtgaacatcc
     6661 gcgaaggacg ccttgtattc cgcggctatt tcacgggcaa ggttcccgag ctgcctcagg
     6721 ttggcattgt attccgccgc cccggtgccg gatttttgaa agaaatttgt gtctaccgcc
     6781 ccgggagacc cgacgatcac ggtcgttacg cccagctcgc gagccttttt tatgatggcg
     6841 cgcatcccgg cttcataatt cttgccgatc gtttcctcgt aaggccggta acggccgtcg
     6901 ttcattccat agcaggtggt catggccgtc ggcttgaaaa acgcgccgag atcgttggcc
     6961 atccgagcgg cgaactcggg ggcggtttcg ccgctccagc cgaactgaaa aacgcttatg
     7021 tcgcgccggc cggcgcaggc caggagatag gtttcgatgt aacggctgta gattttctgc
     7081 tcggtgatgg agtccccggc gattgccaac cgggcgtttc tgggcagcag cggggcttcc
     7141 tgcgcgcgcg ggaaaccggg caacaaggcg gttgccgcca ggagcagcaa ccccgccctg
     7201 attatcatcc gtttcattct tttcctctcc ggggaaacgc gttctttggc gattagatcg
     7261 cctccatggt tgagggcggc ttggtggcga tgttgtaggt tacgctgacc acgcccggca
     7321 cctcgccggt gatgcggccg gccagccgtt ccagggtctc gtagggaagc cggaccggcg
     7381 ccgccgtccg ggcgtcgagg ctcttccagc acctgatctc aatttgaagg ccgaagtccc
     7441 gccggccgcc gcgcatgccg gtcacccggt cctggtgcag gatggccagg tactggaagg
     7501 cttcggtacc ggccagctcc gcctcggtga ttaccgttgc tttccggacc agctcaatct
     7561 tttccggtgt cacttcgccg atgatccggg cggccagggc cggacccgga aagggcatcc
     7621 gcttgtagat ggactccggc aggcccagcg tctgggccac cttgcgcacg ccgtccttgc
     7681 ggatttggcg gagcggctcg atgatccggt agccgaaggc cttctgcgga tcgatgccca
     7741 gctgctcgaa gacgttgtgc tgccgcttga tgccggcgac cgtctcgtcg atgtcggtga
     7801 gaatggtccc ctggagcagg tggcgggcgc cgctctccac caccaatttg ccgaagacct
     7861 tcttgtagaa ggtctgggtg atcgcctccc gcttctcctc cgggtcggtt agtcccttca
     7921 gggcctcgaa gaactcctgg cgggcgtcca cgatcttgac cgggatgccg aatttcccga
     7981 aaatttcggc cacgttccgg gcctcgtcct ggcgcatcag accgttttcg atgaaatagg
     8041 tcagtagacg gttgcccagc gcctggtggc cgagcacggt gaccaccgag gaatcgacgc
     8101 ccccggagag ggccgtgatt gccaggccat ccttgaccag ggatgatatc tcgtcggttt
     8161 tgtccttgat gaagcccgcg acgtccatgc cttcggcctt gatctccttt acttccatga
     8221 ctcctccagt taatgatggg tgaatgaagc aggattgaaa agggttggct gattgacctt
     8281 ccccaaaata ttatcactcc gtcgaggcgc ggtcaagaaa aaattaattt taccgccgcc
     8341 ggggctgtgc ggccgggttg acagccctgg actcaggggg gcgttacccg gatcgagatg
     8401 gcgggcagca tgccgagcga gaccctccgt ttaggacggt ttcccttttc atccggcggg
     8461 ccggacggcc actcggtgaa ggcctcgacg atcagattgt cgaccagccc cggctggact
     8521 tcattggccc cggacttgag catgaaagtc agcaggcccg acgacaggtt gacctcgccg
     8581 agtgaaattc cccggggagg atcgaccagg ctgagcgagc agctcgcgag atgcagacgc
     8641 gggggtaccc gggcgctcac ccggaccatg ccgccggcct ggagattgac cgggccgggt
     8701 tcggccagct cgaaggccgg ccggcgcagc ctggcctgcg ggaccagggc cagcagggtt
     8761 tcggatggca ccaggtggcg gtaggcaaag gcctgcatca tgtcttcagc cggaaccgcc
     8821 ggccggctga ccggctggcc gttgaccagg gcgcgcccct ccagctgaag aaagaacggt
     8881 tccggggaag gttcccgggg cgcgctcagc gtcatgcggg cgctctcccg accggccgga
     8941 atcaagccgc cgttcaggat ccagccggcc ggcgcctcct tgagaagcat ctcgatctcg
     9001 ccgtcaaagc catcccggcg caaggcgtgg acgttgatcg gtgtgacgca tccggccgga
     9061 atggccaggc ttgacggggt cacgcgcagt tcaaaaccgg ggatgcgcgg tccgatacgc
     9121 aggcgatagg catactcctc gccgccatga tcctgggagt cggcgatctc gacctggtag
     9181 gtgccgtccc ggggcagttt gaaggaaagg taggaatcgg cgtgatgggt attgagtccg
     9241 gagccccgat cttcccggtc gtcattccag gccagcacgc gcccggaggc gtccatcagg
     9301 cgaagccggg aatccagcgg cgaaccgagg cggcgagcca tgacttccgc cacgaactca
     9361 aatccggacc ggccttcgaa ctggaaacgg tcggtgtcgc cgggtttgcc gatccggccg
     9421 tttataatct ggggcaggcg taccttctgg gcggccgccg aactgccgga ttcgctttca
     9481 attgattcgg gcaggttgtc caccgagtag agaacgggat tggagatctg tccgttgttg
     9541 ataaaggaga cggtccggat cgtacccggt tcgaaattgg tgtccagcgg cagccggctt
     9601 tccggaaggt tccagccgga gagcgcggcg gcggtcggaa gggcggctcg gccgcccagc
     9661 ggaaagaccc gggtgacaaa gggtgattca ccggcgtaaa gacggtaaac gaagtcctcc
     9721 cggccccggt aaatggagtc ccggatctcg acccggtact caccgtcccg gggtacctca
     9781 taaagtatga ccgggtccgg gtcgaatcgg taatggtcgg cgaaggccgc ttcccggccg
     9841 tccggatcat aaatggcgat ggccgcctgg aaccagccgg gcaccgcgtc ggccagatag
     9901 ggaataagac tcctggcctg ggcccgcaag acaagtttct gtccccggcg cgccttgaaa
     9961 gtgaagcaat ccgagtcgcc cggcatgatc tgcccgttga agaccgccgg gagttcaacg
    10021 gccgttccgg ccggcgcatc cggatcgttc ggttccgtct cgagtatttc cggggccggc
    10081 cccacctgga aacgcagcgg gttggttagc cctccggcgg ccagcagccg cagttcccgg
    10141 tcgccggccg gcgcgtcctc ggcaatcgac aggtccagga tcgccagttc ctggatggat
    10201 cgtttcaccg gctgtttccg gccggggtcc agaaagattt cgtaggcctt tttcagatct
    10261 tcattgctca ggttgtccag gtcgcgcagg agcgggtggt cgggtagctc gaccggcttg
    10321 gccggttcgg tctccgcctc cggctgcttt accggcggga ccggtaatcc gcgccgcgcg
    10381 gccgcttcgg cccggcgctt ctcccggatt gcggtgatcc ggcgccggag ggcatccttc
    10441 tgcagcgagg tcaggggggg tatgtaacga aggttggccg cgctcacccc gtccccggag
    10501 atataggcgg cggacaggcc cctcaggttc tggcctccga ccgtcacctg gacggtggcg
    10561 ccccggcggc cgccggccgg gtaaacatag ccggcggagg gatcagtatt ggcggtctgt
    10621 ccccaggcgc cggccgcccc ggttaatgtc agcagcagtc ccgcgaatat cccgagccgc
    10681 ctgcttccgg gattcacatt atctccctga gaagattccg gctggtaccg ggtgtcggcg
    10741 gaggcaggag gggcaggttg cgtccctggg agtcaggcaa ccggccatca gggtcgatgc
    10801 ccaggagcgt gtaaagactg gcaatcaaat cctggggcgt cacggggcgt tccttgacgg
    10861 tttcaccccg ggcatcggag gttccgacca cgcggccgcc cttgaaacca ccgccggcca
    10921 ccaggaccga gaaacacttt ccgtaatggc cgcggccgcc attccagggg gcttcccata
    10981 agatccgcgg cgtccggccg aactcgccgc agcaccagac gacggtactt tccagcaggc
    11041 cccggccggc gaggtcctcc agcagtgccg aaagtccgtt gtccagttcc ggcagtttct
    11101 ggcgcatgga ctgaaaatgc tgtttgtggg tgtcccagcc gccgtagttg atggttacgt
    11161 aaggcacccc gctttcgacc aggcggcgcg ccaccaggca gctctggccg aaacgggtcc
    11221 ttccgtatcg atcccggacc tcatccttct cggtggttaa atcgaagacc ctggctgcgt
    11281 cgccggtgat cagctcgtgg gcctcattaa ggctgcgatc gaaggaggca aagtacggat
    11341 cggccggcat cgtccggccg agcgtgtcca gcttcttcag gaggtcccag cggcccagct
    11401 gccgttcgct ggagattccc ggtgcggaca ggccctcgac ggcgaaggtt tttccgttgg
    11461 gatccccccc ggtgaccagc ggtttgtatt tggatcccag gaaaccgacc tcggaaaaac
    11521 gcccctgcga ggtggtcagg attatgtagg gaggcagctg cccccggtag ccgtgctcgt
    11581 atcccttgaa gagggctacg accgcgccga cgctcggata ggaaagcgct tcgcccggct
    11641 tgtggccggt ctgggtgatg taggcggccg tttcgtggcc gtcaatgccg tgggtcaggc
    11701 tgcggatgat ggaatacttg tcggcgatct tcgccagcct gggaagcagt tcgttgatct
    11761 ggatgcccgg gatgttggtc tggatcgtct tgtcgaaggg gccgcagtaa tcgtagccgg
    11821 cctccggctt ggggtcgaag gtgtccaggt gcgaggggcc gccccagagc cagatctgga
    11881 tgactgagcg ggcgcggccg cccgcggccg acgggccggc cggttcctcg gccccggcgg
    11941 ccaggctcaa ggcggccagg atcaggagtg ccgcccctga tcttcttaag aatgaggccg
    12001 gccttgaggc ggcgccggcc cgcgccggcc gtccggaccg gaactctttt ccgggtcgtt
    12061 cgatttttaa tggcggcata ggaactcctt gctgttgatc agcgtccagg ccaggtcgtc
    12121 gttggccgct ttgtagccga ggctggtgtt ggcctggaag taggccgcgg caatttccgc
    12181 ttcttccctg gtcggccggc gcgaaaggat aatcaggtag gtctgctcga tgatcagctc
    12241 gcggttccat ttgacggagg ccagcatctt ctgaaggcgg gaactcatag tgattttgcg
    12301 ctggatctcc gacgaattca tcaggcacat ccgctgggcg gccgacggct gccggctgcg
    12361 ctccgattcc agcccggtgt cgcggccggg ccggccgaac atctccagga aggggctggt
    12421 gatgcttccg tcggaaagca ggatcgaacg ctgccgcctg ggcgtaaagg tgaacggctc
    12481 gggtatcggg ctgctgtatt cctcgccgcc gccgcccgcg atccagttca aggcatcgat
    12541 caggacctca gcctccagct gccggaccgg gtagcaggcg aagaaccggg ccgattcggg
    12601 gttgttgccg tcgggtatgg atgattgctg gtaggtgttc gagttcagaa tcgccttgaa
    12661 gagaagtttc aggtcatact tgttgtcgac caggtatttt tcgagcccgg cgagcagttc
    12721 cgggttgacc ggcgggttgt caggccggag gtcgtccggt tcgtggatga ttccgtaact
    12781 catgaaccag gaccaggccc ggttgacgat gctgcgggtg aaccaggggt tgccgggggc
    12841 cagtagccag tcggcgaaga cccggcgcgg gtcctgttcg gggggtatct ttaacctggt
    12901 tccgtcggcc atcaccgttt caatcacggc ggcgggagcc gggtcaagct ggacgatttc
    12961 ctccttccac tcggccgttt tcttgtaggc gatccgtgag aagcaggcct ccagcccggc
    13021 ccgccgttcg gccggccagg attcaaggcg cgtgcccatg aaggtgaggg ccactacccg
    13081 ggcaatcgag ggagcgtcgc gtccctggac cgcccggtaa aagtttaccg gcggcacgcg
    13141 gaaattgctg ccgctggagg tcaggagcgc ccgggcgaag cggtcgtagg gcaggttctc
    13201 ccggagcgag tcccggatcc agcggtgata ggcctggacg gcgttaggcc agagattgat
    13261 ggggaattcc gcctttaccc tcaggatatc gcaccacttg agcgaccagt aatcggcaaa
    13321 ctccggccgc gccagcaggg tgtcaatcag ggcggacctt ttatccggcc ggggatcttc
    13381 cagaaaggcg gttacctcgg ccggttcggg cagggttccg attacgtcca gatagacgcg
    13441 cctgacgaag atttcgtcgg aacagagcgg ggccggcttg attccccgct tgcccaggcc
    13501 ggcgagcacg caggtgtcaa tgaagttggc cggcgcggtc cagccggcgc cttcgaaggg
    13561 cgccgccggc cggcggacgg tatccgcgcc ggacgatgcc ggcgccagga ggaggccggc
    13621 ggcgaggccg agcaacagtg ttttcttcat tttccggggg ccgcggcgtc aacctcgatc
    13681 tcgaagagca ggtcgtcgcg gcagatggtg ttttcaatca ccagcatggg caggtcgggg
    13741 aaattcaggt tggcgagcat ccgttcgaag gccggcgcgt cctcgccgtt tttgagatag
    13801 gtgatcgccc gggtgacgtc ccgccatgcc atccggcgtg attccaggat tgctttcacc
    13861 acatccaggg taagcaaggc ctgtttttca aagttcccgg ggtgggcgct tttcccgtcc
    13921 ggatggatgg cggccgttcc ggaaaccagc agctggcgcc aaccggatcc cgaaatttca
    13981 accgcccggc tgaaggaact gccgtaccgg agggccgggc attggagcgg tgaggcgacc
    14041 gccgatttcc ggatgcttct gtccaggggg cggacggcca gcgcgttggc gaccagggcg
    14101 gcgccggcgg cattgccccc gccgatgccg gtgctggccg gcaccaggcg gtcgaagacc
    14161 cgccgggcgg cgaaaaaacc ggtgcgcacc cggttgaagt cgccgtacca ggccaggatg
    14221 tcgtccaggt agaaccaggt gcggaccagg tcggtaaagg agagcccggc cgcctcaagg
    14281 gcccgttcga tcgattcgaa gaccgcctgg gcctgttttt cccgggacag ggaggtatcg
    14341 gcggcccgga cgtccccgag ccgacagcag cgggaatggc cgtcgtcgta aacgtagccg
    14401 acggtgcgcc cttcgagtcg gatggggcgg acgccggcgc cggtgaccgc ctgcagctgg
    14461 gtgccggcca ggccgtttcc attcccggtt ccccgcagcc gggtgaccgg ccattccggt
    14521 tcaacggtcg ggaccgaact gtttgcggta atcgtcccga aaagatcctg gcccaggatg
    14581 gaggcgttca aggattggac cggggagacg gcccttgcga accaggcgtc cggggattct
    14641 ttgccgccct gggggcggac ggtaatgaag taatgggagg tttcgccgag atcgagtttg
    14701 atcgagtgca gaccggaacc ggcgacgcgt tcgatcatgt gatttcgccc gtcttcttga
    14761 tgatggccgt catggtcgtt tttttaaggt tgatactctt atttcccggg gtttgttttt
    14821 caaaaaattc gatggggaaa cgggagaaaa tgtttattat acaaaaaaaa caacgcgggt
    14881 caaacaggtg aaaaaactca aatataatga taccattcgt tccggccggc ggcaagaaga
    14941 cggtccgggt aaccggcggt ccgggatctg tgctaaaatt tatcggcaac tcttactttt
    15001 ggcgggggaa tcatggtcaa aaccaggata atctgcacgc tcggcccggc ctcctcgagc
    15061 gagaccgtgc ttcggaagat gatgcgggcc ggcatggatg tcggtcgtct caatttctcc
    15121 cacagccgcc ctcgggagct tctccccgga ataaacctga ttcgatcttt gaatcggaaa
    15181 taccgccgcc gcgtccggtt gttgggcgac ctccagggtc accggatcag gatcggcgga
    15241 ctggcggcgc ccctggaact caaaagacgc caggtccttt acctggtccg ggccggggcg
    15301 gccgtcggtg ccggccggat tcccttcgac taccggggtc ctttggaaag catcaaaacc
    15361 ggacagccga tcttcttgga cgacggcaac ctcgtcctgg aggtgaccgc cgtcttcaag
    15421 aatgctttaa agacacgggt ggttgtcggc ggccggctca aaggtcacaa gggcgtcaac
    15481 attccggacg cgaaactgga ttttggcccg atcaacccca gggatgtcga ggatatcctc
    15541 ttctgcgccg agaaccagct cgattacctg gcccagtctt tcgtccggag tgcgggtgat
    15601 atcatggagg tgcggaagat actgggtcgg aattcccgct gccgggtgat tgccaagatc
    15661 gagaaccggg agggcgtcag gaacatcgac gagatcatcg aggcctccga tggcatcatg
    15721 atcgcgcgcg gggacctggg cgtctccctg cctatccagg agataccgat tcttcagaag
    15781 cggttgatca ggaagtgcaa ccaggccggc aagttcgtca tcaccgccac ccagatgctg
    15841 gagagcatga ccgagaatcc gcggccgacc cgggccgagg tgaccgacgt ggccaactcg
    15901 attatcgacg gtaccgatta cgtcatgctt tcggccgaat cggcggttgg aaagtacccg
    15961 gtggaaacgg tggccatgat gaacggcatc atcaagttta cggaagggta tttgaaggcg
    16021 gggaagccgg gtcggtaggg gaattccgga aaatcttttt tgcccgggtt gtgccttttc
    16081 cccgtccgtt gccgggtttc tgccgcgaaa agaaggcagc tcttactttt cactccgagc
    16141 ggagtttctc ttccagcttc tggcgtatca gccggggatc ggccttgccg cccgagagtc
    16201 gaacggcctg gccggtaaga aagccgagcg actgggaacg gcccttgcgg aaatcctcca
    16261 cggcccgcgg gttttcggcc aacaccctgg caagaatctt ctccagctcg ccgctgtcgg
    16321 ccgcctgatc gaggccctgc tccctggcga tctccgccgg tgtccggccg tcttcgaaaa
    16381 gtagttccag cagctgccgg ccggcgttgg gattgatttt cccgttttca accagggcca
    16441 ggaggccggc cagccgttcc ggagtgatct tggtttcggc cagggtcagg ccgcgccggg
    16501 cggcggccgg cagcaggcgg ccggcaatcc actgggcggc cagtcgggcc gggattccgg
    16561 ccctgatgac cgcctcgctg taatcggcgc tctccgggga gacggtcagg ccggccgcct
    16621 ccgtttcggt caatccgtac tcttcgacga agcggagctg cttccgggcc ggtatctccg
    16681 gcaggccgtt cttgatttcg gccagccatt ggtcggagat gaccacctcg gggaccgagg
    16741 gatcgggaac gcaggggccg gaaaactttt cgcgcatagg cagggtccgg ccgcttttcg
    16801 ggtcccagag acgggtgtgc aggatgatcg cctcgccttt ctggatcaac tcctgctgcc
    16861 gcttgatctc gtaggcgatg gcgtccccga tgccccgcac cgagttcatg ttcttgacct
    16921 cgaccttgac gttcaattcc tggctgccgg ccggccgcaa cgagatgttg gcgtccaccc
    16981 gcatcgtacc ccgttccagg ctgcactcgg cgccgccggc tccggggacc agggtgcgga
    17041 tcgcgttgac gaactccatg gcttcgtaag ggctgcgcat gtccggctcg gtgactatct
    17101 cgaccagggg acagccggcc cggttgaagt caaccaggct gacgcgggtc ttgccctcgg
    17161 tttcatggac gagcttggcg acgtcctctt ccaggtgaag ggtgcggatg cgcaggcgac
    17221 gcaaggcgcc gtcctcgccg gtaatctcca cccagccgtt tccggccagc ggtcggtgga
    17281 actgcgagag ctggaaacct ttcggcaggt cgggataata ataaaccttc tggtcaaaac
    17341 ggctacgctg gttgatcgtg caattcaggg tcatggcgac gccgaccgcc ttttccacca
    17401 cctcccggct cagttgagcc gaggcgcccg ggaaccagag gcagatggga caggtattct
    17461 ggttcggttc gtctccgaac cgattgggac aatcgcagaa catcttggtg gcggcgttca
    17521 actggacgtg gatttccagg ccgacgaccg cctcgtattt tgtcgtcatg cgccacccgt
    17581 cagggtttcg aaaagccgtt tcccgcaggc cagcaggaag gcgtcggacc gaaacccgcc
    17641 cattacctga agaccggggt cggacgcctc ccggccgccg gagagggaaa aggcgagggc
    17701 ggggcagccg gtgacgttgg ccggcagggt gagggagaag gcggcgtagg tctgatcgac
    17761 ttcccgggcc gattcggccg ccggcgtttc cggccggacc ggactgatga tcaggtcaac
    17821 cgtctcgaat agttgaccga tttcccggat caggcgggcc cggatccggg ccgcccgttc
    17881 gaaggttccg tatgactcga actggaagaa ggcgccctgg aagagcagcg tcttcaggaa
    17941 cgtgctgaag gaggcggccc gcgtcttcag gtacatgtca ttccagttgt tagccgaatc
    18001 ggcccgcaat ccgtaacgga cgccgtcgaa ttttccgcag ctggaggagg cctcgacgct
    18061 gccgatcacc cggtgcaggt cgcggaaagt ttcgtctccc ggccagggaa cttcccgaac
    18121 cgggaatccc gccccttcga gtttcttgag gccggctgaa aagatttccc gggcggccgg
    18181 gctgaggcgg gcttcgcagc cggcgggccg tccgaggacc ggtttggatt ccgttttcgg
    18241 cggccggacc gggaatccgg ccagcggttc cgcctcaaga gagaaatctt tgccgtcgaa
    18301 accggcgatc tcggccattg ccgcggcgac gtccgccgga tggtttccga gcaatccgac
    18361 ggcctcgagg gagggcatca atccgatcag gccggcccgg gagacggttc cgtggctggg
    18421 tttgaaaccg aagcgcccgc cggcggcggc cgcgtagcgg ggttcgccgg ccaggtcggc
    18481 catgaaggcc aggtagtcgc ccagcnnnnn nnnnnggccg cctggctggt gaagttcttg
    18541 agacaggcat cgccgttcag cccgaagccg aactcggcgg tggcggcccg gccggccaga
    18601 acggcgccgg cctccaggct gcgctgaaca accgttgcgt tctccagggc ggtgaacttt
    18661 tccagggccg gcgatccggc gtcggagacc tggtcccgga ccgaaaaatt ggccggcgcg
    18721 atgagcttct tgtcccggag gcggtccggg ttttcagatt cgctgatctt cattccagga
    18781 tattggccac cttgaaatgg ccgtttttga ccgcgggggc gtttcggaag agatccttct
    18841 tttcaacgcc cgggtacttc gaagccgtcc ggccggggcg catccggttc ccgggccggg
    18901 agggcgagta gaccggttcg gcgtcggcgg gaagtttctc gatccgtccg gccagatcgg
    18961 cggccgcttc gacggccttt ttcagctcgt ttctttccga ttcttcatac ccgaggcgcg
    19021 aaacccaggc gaggcgtttg acggggtcct cttcccggcg gccggagatt ttcagctcgc
    19081 cttcaccggc caggtgtagt tccgccagct gttccggggt gaccggggcc ggggcctggg
    19141 tgagcgggca gacggcgctg cggttcttgg gaaaggcgat cacgtcgcgg atcgaggcgg
    19201 ttccaaggat catcg
//
LOCUS       DAOQYN010000009        19158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_3240_length_19158_cov_5.120191, whole genome shotgun sequence.
ACCESSION   DAOQYN010000009 DAOQYN010000000
VERSION     DAOQYN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 19158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19158
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3240_length_19158_cov_5.120191"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..2433
                     /locus_tag="PKN80_00840"
     CDS             178..2433
                     /locus_tag="PKN80_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNQ34595.1"
                     /translation="MKFKQLLFLSLLALAALTGFAAPGAGDETSAKKPDPAAAAAPGH
                     IYYVSSKGSDANDGASVKTPFASIAKLNGVLAAGDTARFKRGEIFFGTLRPKTSGTAA
                     APIRIGAYGTGARPIITGFRRITGWKAAGPGLYQATLDPAPTGRLEMIRFDGRLQPKG
                     RLPKAGRGWKPGSDTDSWFEIRSHLANREITSADAVPDVAGGEIVQKKYQWIIDRGRI
                     DTVLTNNVDGQTLRTIKITEYPTLYDNPYHAFDKNGFFVQNHVSCLTEPGDWCYDETT
                     RRITMHFGAGPPDRHVVEAAALDNAVDLTNRAYVNLEDLALTGANGDLVRLDVPDNLT
                     GKCHRVSINNCDLAFAGRNGVAVIEHRFTTYTSDAADGSITNCTISEINNNGISLGEN
                     PRWTITGNTLSRIALNEGLQQNSDGQGIGIFAAGADSLIQHNEISQVGYSGLYFVGSG
                     IQIRNNHIHHFCLVKSDGGGIYTYGGETRKTHAKPRVIDRNVVHDGVGSIEGRHVSEA
                     GNPYTPQCQGIYMDGNATDVIITGNTSFRNASAGLSLGSNSRITVYGNTLADNHTTQL
                     QIDDQKSPPANLDIHDNILFAFEREQLCLSVGLARKPGNLTQAQYVAAIGTLADNLYL
                     RPVREPENISTIGYPHCGDGGGHDPNRSHANYGVFNDYPGGGIIATTWNGSPFLSLDR
                     WQKEWGQDAGTRKTFRSVAGIADLRLEYNPTDAAAVKKLPGVFEDARGAVYKNSLTLP
                     PYSSAVLMRKP"
     gene            complement(2761..3270)
                     /locus_tag="PKN80_00845"
     CDS             complement(2761..3270)
                     /locus_tag="PKN80_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNQ34596.1"
                     /translation="MKKIFSYLLLLAAIGIAIPLLLKAQASRSSFVRVINIFDGDTIK
                     VTYQGKPEKVRLIGVDAPELRHSTRSVMYYAQEAADFTERMVKGKRVRLEFDENNKYI
                     QNRDKYDRLLAYVYLEDGFFLNAELLKRGYGFAYTEFPFKYQDEFRRYQKEAAEGGVG
                     LWGKHKKKR"
     gene            3540..6350
                     /locus_tag="PKN80_00850"
     CDS             3540..6350
                     /locus_tag="PKN80_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34597.1"
                     /translation="MKSLLKNKKRNFAMKFYTSLIALFFGMSFGASAATITVDDSGGA
                     NYTTIQDAINNAVAGDTILVAAGTYNQKISIVGLDGLTVKSANGAATTIINLPDSIAP
                     GGVFINCNNVTFDGFTIRDFSDNTAEDKIIRIGASPAVADGNVVKNCIIQGNLNQPGV
                     AEQTDYGIILYGTNCQIIDNEIFDIGYIGINVVGVPHSDAGGNTISGNNLHHMMRAIT
                     LDRSPGNSILSNNQISNLVAGTLWGEEYPPERYCYAIICWGSPLAGTVIENQNITGVP
                     NGISLSSACGVILVNNTLEVTNIGISMSYAGWGDNPPGTMPAPDNNIIAGNTITGCDK
                     ALNIGATNIGTNNFFGFNNIEGNSMGVNNTGTEEFVAEKNWWGDATGPFNGTTNPGGL
                     GNEVSDNVDYTPWLGAEAADLMVLNSEEPSGENGEDGIAVDVDFDGDEGTVLVTVYKD
                     DPVGNAPFEGSFFDVFIAQMGDIEEIKVRLYYNEPSAKVAYWHNGAKWLVCSAQEVIA
                     GAVMLENVMYDGYVEVTINDGTFPSLADLTGTVIALRDTPLYDSVSGQSTCFIATAAF
                     GSPFERHVQILREFRDRYLLTNTAGRAFVRWYYRHSPRYAAVIAGNTMLKAAVRIALM
                     PAYAVALIAVKGAVPYLLLGLGLLLLALRKKTAKSAAILAVAVILGFPAVSYTADGNH
                     FDITPGDKYTATVPTTSTIGASKWAFDVFYSYTNKMLEADLAGTEMDLVKEQHLLQAA
                     LTFGISDLQQMSLVVPYVAQQRSEIDGTGRSGFGNLSLVYKFRFPTENENGLKAAVAP
                     FLQLDTKKNDIYLNSDDYVLGIRGIVDKQINENLLWTGNIGYAYQPEEKLGDNVKIEH
                     TLLFGTGLVFKIPETRSYVSAELFGRSEDMFDTENTPVEGILSYGLVGENATFTIGAG
                     IGLVDGYGAPDWRVFTGLRFGI"
     gene            6576..7322
                     /locus_tag="PKN80_00855"
     CDS             6576..7322
                     /locus_tag="PKN80_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNQ34598.1"
                     /translation="MADFKLDLAVQSYTFRGFKDNTKVAGFLNELGLKKIELYGAHAS
                     PADPAAFAAAVETYRKAGVEVGSIGVYGFGDQAEKNRPIFEAARKAAIPLISADFELK
                     SFPNCIRVAGEMAREYGIKLAVHNHGARHWLGSAQMLSHFFGLAGPEIGLCLDTAWAL
                     DSREDPLAMITAFKERLYAIHLKDFLFDSARKPQDVVVGTGNLDLKALFAKLAEAGFS
                     GPAVLEYEGDVEDPRPALKKAIEAIAAAVK"
     gene            8113..8573
                     /locus_tag="PKN80_00860"
                     /pseudo
     CDS             8113..8573
                     /locus_tag="PKN80_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
     gene            8597..9304
                     /locus_tag="PKN80_00865"
     CDS             8597..9304
                     /locus_tag="PKN80_00865"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNQ34599.1"
                     /translation="MENGQEAYRMPMIGEKAPAFTAKTTQGIINFPDDYKGKWVILFS
                     HPADFTPVCTTEFMTFASLQGDFKKLNCELIGLSIDSGYSHIAWLRTIKEKIQFRGMK
                     DVEVTFPVIEDLKMEVARKFGMLQPSASDTQAVRAVFFMDPKGIIRAIIYYPLSNGRN
                     FKEIKRLLIAMQTSDRFGVATPADWEPGEDVIIPPPGSCGLAKERVEKAKQDGYYCLD
                     WFLCFKKLPKSQLEAKK"
     gene            complement(9377..12901)
                     /locus_tag="PKN80_00870"
     CDS             complement(9377..12901)
                     /locus_tag="PKN80_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34600.1"
                     /translation="MRQRKLLRGACLAFFLLLGLAVEAGALSLFTEVEKFNNLDYVGN
                     WVDMPGWYVKRHYHASGGAFAVTHHPARMYLKLDNPVPAGSYRLLLRVCLNRSANTRN
                     LLKASLGNYDPAGRFTANAAAPFVQDFTGSGYGWYELAGVLKTGLPCQVIEIEAVTVE
                     NCGIGDDPEYQRPYAILDSLVITDEPVEIIKDTRRGRNELKFAGADPRQSAERIKYPS
                     APAAVAFENLPPAREPYFPARNLLRNSSFEFSVKPFYTGAGDTMTSGNNVDNDCLDAE
                     RPYHGRYSLKLVPRPQNLLEDYTGSKASIRYGMAFNVFYLNQFLATEFKKSVTGPLTL
                     SWYLRTNGRPVSVNGKTVSHTDWQRYTVEYTPAASISFTTDDPQALVWVDALQLERGR
                     QASDYQPLDGLEVGIQTGVPGNRFYRGRNLPLEISVSQAVGQRPERLGINYRILGPHL
                     EVLVSGRQSVQSRPGGVVALPVKPAFDRLGNYLLLWEVEKRPELGYAIPFQVIEDPKQ
                     FAGSGPQLGAIISTNEEIMKIFKQAGFDWVNSLNDRMAYFDYIWPSREEMRFFDRYYR
                     MWGEKYGIQYSFWRPHFNPPKFTENILGVEPAAHMDGPNIPYQDWAEFWERFTSRANY
                     IKAWQPTDEQSYHRGPKESLPYVEIANRIIRKNVPGAVIMNSTQITHLLEMLNLNPKL
                     DIGDAIGGSRHGFERNLFNYDRYAKDFTGKQYWVVGVGWTTGDWGSVLNPETFQPRRD
                     YWSGRWLNGNRSVIYDVFNEAAVVGTERFGLYTAKFDGGRDPFALFAGDNTLRPWSAN
                     FINAVNFLRGHRPDGLILMETAYGLDAAGLYRKNGRFCVLLCPDGSYGNIEVNLDLPA
                     RQVALYDYQLNPVSFSPKLVLPVSKLLVIEDNGLGRDKLLAAIRSLKAYPVDLERRVV
                     LPAGPGLELATFKMIKGRMEKVSGQPFPAGAGFSEPIDQGQYERNASWATVAGLVGAG
                     TVKVDGRLDEAAWVKAAPSFIYCWSALDGSYGALQGIQNFSRIFELEDASVSFKSLWD
                     GRDAYLAFEVLDGVRRPGDGLVVKLDRDLLDDLGNPLPDADDLSITVALAGGPQQPAV
                     IDGQGREIGRCEAVVVDTDEGRRVELRLPSELIGTAGRQERALGLNVELVDQDGDGPA
                     AVLSWTGNHLPRRSPFGYGQLILSGVAR"
     gene            complement(12934..13710)
                     /locus_tag="PKN80_00875"
     CDS             complement(12934..13710)
                     /locus_tag="PKN80_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNQ34601.1"
                     /translation="MKRLIPLLALALLALAPAAGAAEPGLVLHYSMEEAVDGVVRDLS
                     GNNNHLKLGPGAALADGQFGKALVLDGKGAILVPDSPSLRSIKNGLTVSLWLRLDTDA
                     IRWTSFVKKDAATEATGKGGGPTAYRPFRGFNLGTNGSSGPTKIKEGAGFLFTVDGVG
                     GRDTVSVPMAKYVEPGRWVHLAATYNTGSRDRGLRLFVNGVLIGDIVMIEMPTDISTE
                     TPLTIGRDLTGLVGALDEIRIYDRPLPEAEIKKIFQSAGK"
     gene            complement(13719..15866)
                     /locus_tag="PKN80_00880"
     CDS             complement(13719..15866)
                     /locus_tag="PKN80_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34602.1"
                     /translation="MIRKLLCLTAVLFCFGGRPAAAVNLIPNSGFETGAYRLWVASYQ
                     SARLVDDSDLDFQVFHDGTASLRLTAWSAGTGYSLSPFLSLTSAFTVPVPGAGTYTLS
                     AWVKSDRKLNFNLACGAIKIQVVVLPEEGWRRVTASGPLDKPEKLAVAVSGSDYDPNQ
                     LHAGHLWLDSLQLEAGETAGAYAPAGPELRLVCLPPANIFHDSGPVEVLLRGVNPTGR
                     DVKVDLEGRLWDCFGREFPVTSARASFLLKKDGVSEFKYAFPKERKGVFRFQVLAREA
                     GGGTFGSGLRMEIPFYVVPRPDPDGWNPFGLYLQLSVPGLERATRLGLYWNNTLSSAG
                     ATTEWKKIYARDQYWFEAYLPYLKAARDRYRMRFVGCVSDAGFIDVFPKMAEAEKSIP
                     GETIDLESANGPRYLKISAFREYLQTMARHYSPYIKYWQMIDEVPFGGPAYLQLMVEG
                     ARALKSVDPKAVLLATYPRNAALAYVRGGPESTGGLYDLGRELGRVRYSVKAADLAGP
                     DFPLFFYDCSVLMNYHSDHFNGWGKFASRLGRTAEGVEVDSCLSDFTSRFNDTLVRNL
                     RPAGGGGPRVKAVIIYHGRLPGGGYQSTFDLWGHLDPSIVALGCFNGLTAAGASEGEL
                     GLPWEGFVFSLGQGKGKLLAVMPGKPAAAEVEVPSGLNLRQLDIWGNDITLNRAGKRY
                     LALVQPYAYVYLTLPDNQLAAAREFLAGLAASK"
     gene            complement(16038..17612)
                     /locus_tag="PKN80_00885"
     CDS             complement(16038..17612)
                     /locus_tag="PKN80_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34603.1"
                     /translation="MQRIMRTWILSLFLAGFFLSVLPGYAAGQVARAPGLGRYDEQYG
                     YPAPKWFQELEVVDAVSSDPATAGKLEFSHPSGGFQAAKAGWYAAGNGWPNAGLETRQ
                     AWNAAGSRASAYQDNPMFGIIEHELGMKFVREHGITRTEDERGDTPRGELAIKIRWLP
                     GQQGWPGKYVNKDGREARWNLYDPDGNWVWKWGFYPGRHITCVNLKEVQDAWKLAIRS
                     LMELGYDGIMFDNYQKSPPCFGDKLGKHRHLFPDRTNAEVLDDLYWELYKVIKGYHPD
                     RVMWANSQGLAPWRLVDGQLIEGFMHEVTDGSAAEVCAYVVKTARRYQEAVEHGKVML
                     YMPRVPESIPGDKEDRAFFAYVAARLGRYVLAWPAAGLDKINWLRLKQVRSGIVEVAE
                     GVYLRAYERGVVVLNGTGQPRAVKLPLASKTLKAAGGLVDVRTEQAYPVKKGAAAIEV
                     VVPAYSGRVYATKAAFDLARARRLPDKPGTHAAPLLPGLKVAPADVILQGPEAFRKWH
                     QDNPDAKKYNLFDGPR"
     gene            18087..18335
                     /locus_tag="PKN80_00890"
     CDS             18087..18335
                     /locus_tag="PKN80_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34604.1"
                     /translation="MLLLDSLLLMIMEYVLLGSPPTTCGDRHEGMTPEAFDRLRLGKA
                     KYKKLGVHFRALDWLFRRISKSPSCPYVVDGYPFLKRF"
     gene            complement(18441..>19158)
                     /locus_tag="PKN80_00895"
     CDS             complement(18441..>19158)
                     /locus_tag="PKN80_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ34605.1"
                     /translation="GFTLIELLVVVAIIAILAALLLPALNAAREKAYAAQCMSNLKQL
                     GTTMRIYWNDNDDYLLASWWGDVGVSRPSYPEMLYNENYVKNQEALRCPKFGTKAMDR
                     RAYCYPSRGSMGEGYYTGTSGGSGLFLFVFDYPANGNWPNKMATTRYRAKDVRRPERL
                     IFSMCGTLAGNTNIGNYQYTFSSVGSNGTSYYSPGSYYGLCTGVPTHRGGTNFLFYDG
                     HVEFLTTDQCKDTNYWYNNP"
BASE COUNT         4032 a         5701 c         5877 g         3548 t
ORIGIN      
        1 cgagcaagtt gagggctatt ccgttcgggc gtccttcctc ttcccgagtt cctgcttcca
       61 ggagagggtt gggcgcaaga ccggattaag catttcccaa tcctctcctt ctttttggcc
      121 ggggtcaagc agaatccagc ggcaccttcc cggcgcccca atcgaaagga gacccccttg
      181 aaattcaaac aattgctctt cctgtcacta ctggcgctgg cggcgctgac cggctttgcg
      241 gcccccggcg cgggagacga aacatccgct aaaaaaccgg acccggcggc cgcggcggcc
      301 cccggccata tttattacgt aagctcgaaa ggctcggatg ccaacgatgg cgccagcgtc
      361 aaaacgccgt tcgcctcgat agccaagctc aacggcgttc tcgccgcggg tgataccgcc
      421 cggttcaaac gcggcgaaat cttcttcggt accctgaggc cgaaaacgtc cggtacggcg
      481 gccgcgccca tacggatcgg cgcttacggc accggcgccc ggccgatcat caccggcttc
      541 aggagaatca ccggctggaa ggcggccgga cccggacttt accaggcgac gctggatccc
      601 gccccaaccg gacggctgga gatgatccgc ttcgacggcc ggctgcagcc caagggacgg
      661 ctgccgaagg ccggccgcgg ctggaagccg ggcagcgata ccgacagctg gtttgaaatc
      721 aggagccacc ttgccaaccg ggagatcacg agcgccgacg ccgtccccga cgttgccggc
      781 ggcgagatcg tgcagaaaaa ataccagtgg atcatcgacc gcggccgcat cgataccgtc
      841 ctgacgaaca acgtggacgg gcaaaccttg cgcaccatca agatcaccga gtatccgacc
      901 ctctacgaca acccctatca cgccttcgac aagaacggct tcttcgtcca gaaccacgtc
      961 agctgcctga ccgaaccggg cgactggtgc tatgacgaaa cgacgcgccg gatcacgatg
     1021 catttcggag ccgggccgcc ggaccggcac gtcgtcgagg ccgccgcgct tgataacgca
     1081 gttgacctta ccaaccgcgc ttacgtaaac ctcgaagacc tggccctgac cggggccaac
     1141 ggcgacctgg tccggttgga cgtcccggac aaccttaccg gcaagtgcca ccgcgtttcg
     1201 atcaacaact gcgacctcgc cttcgccggc agaaacggcg tcgccgtaat cgagcaccgc
     1261 ttcaccacct acaccagcga cgcggccgac ggctcgatca ccaactgcac gatctccgag
     1321 atcaacaaca acgggatcag cctcggcgag aatccccgct ggacgatcac cggcaacacc
     1381 ttgagccgga tcgccctgaa cgaaggcctg caacaaaact ccgacggcca gggcatcggc
     1441 atcttcgcgg ccggggctga cagcctcatc cagcacaacg aaatttcgca ggtcggctac
     1501 tccggcctgt acttcgtcgg aagcggcatc cagatcagga acaaccacat ccaccacttc
     1561 tgcctggtca agtccgacgg cggcggcatc tacacctacg gcggagaaac gcggaaaacc
     1621 cacgccaaac cccgggtcat cgaccgcaac gtcgtccatg acggggtcgg cagcatcgaa
     1681 ggccgccacg tcagcgaggc cggcaatccc tacacgccgc agtgccaggg gatttacatg
     1741 gacggcaacg ccaccgacgt gatcatcacc ggcaacactt ctttccgaaa tgcgtccgcc
     1801 gggctgtccc tgggctccaa cagccgcatc acggtctacg gcaacacgct ggccgacaac
     1861 cacaccacgc aactccagat cgacgaccag aagagccccc cggccaacct ggacatccac
     1921 gacaacatcc tctttgcctt cgagcgcgaa caactctgcc tctcggtcgg cctcgcccgc
     1981 aaaccgggca acctcaccca ggcccaatac gtcgccgcca tcgggaccct ggccgacaac
     2041 ctttacctgc gtccggtccg cgaaccggag aacatcagca cgatcggcta tccccactgc
     2101 ggcgacggcg gcggccacga tccgaaccgg agccatgcca actacggggt cttcaatgac
     2161 taccccggcg gcgggattat cgcgacgacc tggaacgggt cgccgttcct gtcgctcgac
     2221 cggtggcaga aagagtgggg gcaggacgcg gggaccagga agaccttccg gagtgtcgcc
     2281 ggcatcgccg atctccgcct cgagtataat ccgaccgatg ccgccgcggt gaaaaaactc
     2341 cccggcgtat ttgaagacgc caggggcgcc gtctataaaa acagcctcac gcttccgcct
     2401 tactcctcgg cggtcctgat gcgcaaaccc tgaccggcgg aaggatcgaa aacccggggc
     2461 gggcgaaaaa ctctttaacc ttgacaacgc gcggccgggc cggtataagt aatagtggaa
     2521 gcgggaggaa cgggaaacag acgagacaaa ccgaatcccg ataaagttga gggctactcc
     2581 attcgggcgt atttcccgat cctgaaaccc cgcttccaag agagggccgg ggggtggaca
     2641 ggtttaagcg tgacccgacc ctctcttttt tttgtggagg ggggaaccag gcggctccgc
     2701 gccgccgcgg cgggcaggaa ggacggaaaa attttcagcg cacggaagac aaaaccgcgg
     2761 tcaccgcttt ttcttatgct tgccccaaag gccgacgccg ccctcggcgg cctccttctg
     2821 gtaccggcga aactcgtcct ggtacttgaa ggggaactcg gtgtaggcga aaccgtaacc
     2881 ccgctttaaa agttcggcgt tgaggaagaa gccgtcctcc aggtagacgt aggccagcag
     2941 ccggtcgtac ttgtcgcggt tctggatgta cttgttgttt tcgtcaaact ccagccggac
     3001 ccgcttgccc ttgaccatcc gttcggtaaa atcagcggct tcctgggcat aatacatgac
     3061 cgaccgggtg gaatggcgca gttccggcgc gtccaccccg atcaggcgga ccttctccgg
     3121 cttgccctgg taggtaacct tgatcgtgtc gccgtcgaaa atattgatca cccggacgaa
     3181 gctggaccgg gaggcctggg ccttcagcaa aagggggatg gcgatgccga tcgccgccag
     3241 cagcaacagg tagctgaaaa ttttcttcat ggtccgatct ccagataaat accgggtata
     3301 actgtcataa tttatcaccc ggagaggtaa ataatcaagg atgccgcggc aaaaagtgaa
     3361 aaacaggaag cttttgcgga ttttcatttg acaagtaagg tatagtggag tataattatg
     3421 ctcggcgcat gagaaggcag aggtaaaaca gttctttttg aaagtcaaat gaagtatcag
     3481 ataagcagat aaaacgatat gcacattttg tgcaaattta tgcaaaggag gaaaacagaa
     3541 tgaaaagttt attgaaaaat aaaaagagaa acttcgcaat gaaattttat acttcgctta
     3601 ttgcgctgtt ttttggtatg agtttcggag cgtccgcggc gaccattaca gttgatgaca
     3661 gcgggggagc gaattataca accatacagg atgcgataaa caacgcggtt gccggcgaca
     3721 cgatactggt ggcggcaggc acctacaatc agaaaatttc tattgttggt ctggatggat
     3781 tgacagttaa gtctgcgaac ggtgcggcta ccacaatcat aaacctcccg gattcgatag
     3841 cgcccggcgg agtttttatt aactgcaaca acgttacttt tgatggattc acgatcaggg
     3901 acttttccga taacacagca gaagataaga ttatccgcat cggggcgtca cctgctgttg
     3961 ccgatgggaa tgttgtcaaa aattgtataa tccagggcaa tctgaaccag cctggagttg
     4021 cggaacagac agactacgga attattctgt acggtacgaa ctgccagata atagacaacg
     4081 aaattttcga tatcggatat atcggaatca acgttgtcgg cgttccccat tcggatgcgg
     4141 gaggcaatac aatcagcgga aacaacctgc atcatatgat gcgcgccatt acgcttgaca
     4201 gaagcccggg aaacagcatc ctcagcaaca accagatttc aaacctggta gccggaactt
     4261 tgtggggaga agaatatccc ccggaaagat attgctatgc cattatttgc tggggctcgc
     4321 cgcttgcagg aactgtcata gaaaaccaga atataaccgg cgtacccaac ggcatctctc
     4381 tttcttccgc ctgcggagtt atactggtaa acaacaccct tgaggttaca aacattggga
     4441 tatcgatgag ttacgcgggc tggggagata accctccggg aacgatgccc gcgcctgata
     4501 acaatatcat tgcaggcaat accattaccg ggtgcgataa ggcgttgaat ataggagcaa
     4561 ccaatatagg caccaacaac ttcttcggat tcaacaacat cgaaggcaat agcatgggtg
     4621 tcaacaacac cggtactgaa gaattcgtcg cggaaaagaa ctggtggggc gatgcaaccg
     4681 gaccttttaa tggaaccacc aaccccgggg gactgggcaa tgaggtaagc gacaacgttg
     4741 actacacccc gtggctggga gcggaggccg ccgacctgat ggtattgaat tcggaagaac
     4801 cttccgggga aaacggagaa gacggcatag ccgtcgacgt tgattttgac ggagatgaag
     4861 gaaccgtcct cgtcaccgtt tacaaagacg acccggttgg aaatgcgcct tttgaaggga
     4921 gcttttttga tgtattcatc gcgcagatgg gcgatataga agaaataaaa gtacggcttt
     4981 attacaacga accgtcggcg aaagttgctt actggcataa cggtgcaaaa tggcttgttt
     5041 gttctgccca ggaagttata gccggagcgg ttatgctgga aaacgtgatg tatgacggat
     5101 acgttgaggt aaccataaat gacggaacgt ttccttctct ggcagacctg accggaacag
     5161 taattgctct cagggatacg ccgctgtacg attccgtttc cgggcagagc acctgtttca
     5221 tagctacggc ggctttcggc tctccatttg aacggcacgt gcagattctc agagagttca
     5281 gggacaggta tcttcttacc aataccgcag gacgggcgtt tgtaagatgg tactacagac
     5341 acagcccgcg gtatgccgcc gtaatagcag gaaataccat gcttaaagct gcggtaagaa
     5401 tagcccttat gcccgcctac gcagtagcat taatagctgt taaaggagct gttccgtacc
     5461 tgctgctcgg attggggctg ctccttttgg cattaagaaa gaaaaccgcc aagtctgcgg
     5521 caatactggc agtagcggtg attcttggat ttccggctgt ctcatatacc gccgacggca
     5581 accattttga catcacgccg ggcgacaaat acacagcaac agttcctacc accagtacga
     5641 tcggagcttc caaatgggct tttgatgtat tctattcgta cactaacaaa atgctggaag
     5701 cagacctggc gggaaccgaa atggacctgg tgaaggaaca acatctgctg caggccgcac
     5761 tgaccttcgg catctccgac ctccagcaga tgtcgcttgt agttccctac gttgctcaac
     5821 agcgttccga gattgacgga acagggagaa gcggatttgg gaatctttca ctcgtttata
     5881 aattccgatt tccaaccgag aatgaaaacg ggctcaaagc agccgttgct ccatttctgc
     5941 aactcgatac caagaagaac gatatctacc tgaattccga tgactatgtt ttgggaatcc
     6001 gcggaatagt ggataagcaa attaatgaaa atctgctctg gacaggaaac ataggttacg
     6061 cctatcagcc ggaagaaaaa cttggcgaca atgtcaagat agaacacacc ctgcttttcg
     6121 gcaccggcct ggtcttcaag attccggaaa cccgttccta cgtttccgcc gaactgttcg
     6181 gcaggagtga agatatgttt gacacggaaa atacgcctgt ggaaggcatc ctgtcgtacg
     6241 gattagtcgg ggaaaacgca acctttacaa tcggcgccgg tatcggattg gtggatggtt
     6301 atggcgctcc cgactggaga gtattcacag gattgcgctt cgggatataa agcattgcat
     6361 agtctgtacc agtcggcacc aattcttatt agagtttgaa acactcccgc gcggctggga
     6421 tttctgtctg tttggcaaac cttccaggtg gtaaacctcc aggtgcacga ggcggccttt
     6481 ccttgcggcc ggaattctga tagactattt acgatggccg cgcccggcgt caggcggccc
     6541 cgcgcaatcc cctttcaact caacctggag gagtgatggc tgacttcaag ctggacctgg
     6601 ccgttcagag ttacaccttc cgcggcttca aggataacac aaaggtggcc gggtttctga
     6661 acgaactggg cctgaagaag atcgagctct acggtgccca cgccagcccg gccgacccgg
     6721 cggccttcgc cgccgcggtc gagacctacc ggaaggccgg ggtcgaggtg ggctcgatcg
     6781 gcgtttacgg gttcggcgac caggccgaaa agaaccggcc cattttcgag gcggcccgga
     6841 aggcggccat cccgctcatc agcgccgatt tcgaactgaa gagtttcccg aactgcatcc
     6901 gggtggccgg cgagatggcc cgggaatacg ggataaagct ggcggtgcat aaccacggcg
     6961 cccgccactg gctcggctcg gcccagatgc tgagccactt cttcggcctg gccggaccgg
     7021 agatcggact ctgcctggac acagcctggg cgctcgattc gcgcgaggat ccgctggcca
     7081 tgataaccgc cttcaaggaa cgcctctacg cgatccacct gaaggacttc ctcttcgatt
     7141 cggcccgcaa accccaggac gtggtggtgg gcaccggcaa cctggacctg aaggcgctct
     7201 tcgcaaagct ggccgaggcc ggcttcagcg gaccggcggt gctggagtac gagggcgacg
     7261 tggaggaccc gcggccggcc ttgaagaagg cgatcgaggc catcgcggcg gcggtgaagt
     7321 aagaacaaca acggttctgg aagaaaagag gcggccgggg agaaacatcc ccggccgttt
     7381 tttttgccgg gctatccata gcccgtcatg cccgcgggct tgcccgccga agctcatttt
     7441 ccagcgtagg agaatagttg gcgggtatcc aacaagcacg tcatacccgc atgttgtaga
     7501 cgggtatcca atccttaaga ggggttgact gataaatacg tggattccct cctactacat
     7561 gagggaatga caagcattta tggatccccg cctacgacgt gcggggacag gcatgagggg
     7621 atgacacgca agaaaataaa aacatttcat gcccgtgtgc ctcaggcggg cttgcccgcc
     7681 gaagcttgtt ttctgagcgc aggcgggcag ccaatcctta agaggttgaa atcaaaaaca
     7741 acggattaaa gacaactact ggatttcctc ctactacgag agggaatgac atgcaagaaa
     7801 ataaaagtgt gtcatgccgt gtaccgtaga cgggcatcca aaacgcctgt catacccgca
     7861 ggcttcaggc gggtatccaa tccttaagag gggttgaact aaaaagaatg aataaagaca
     7921 actactggat ccccttctac tacattaagg ggatgacatg catttatgga ttcccatcta
     7981 ctaccccggg gagatgggta tccgcgggga ttccgcagga gcgccggtgg gaaagaagat
     8041 ccgcttgaca ggtaagcgcc gacccggtat aattaataat gattattttt taataattat
     8101 tttctaataa ttatgacaac tattaacgcg gtagcgagtt acatcgaggg actgaagcgg
     8161 gccggcttga ggctgacgcc ccagcggctg gccctctgcc ggcagctggc cggaaaccgg
     8221 gagcacccga ccgcggccgc gctctaccgg aagatgaagt cccgtttccc gacgctgagc
     8281 ctgccacgat ccacaaaacc ctgaataccc tgcaagagtt gggccttgtc catgacctgg
     8341 gtgacaccgg cggcggttcg gcgcgctacg acaccgacct gaaaccacac gcaaacgtcg
     8401 tctgccgccg gtgtcactgc attgaagata ttttcgacga gaacttgatc ggcctgcgcc
     8461 ggaaggtggc caaggcctcc ggttttgaca tccggggcga acgcctcctg tattacgggc
     8521 gctgcccgaa gtgcgccggc ctcacgaaga aaagacaggg taaaagcggt tgaacgccaa
     8581 aaaaggagta gagaaaatgg aaaacggaca ggaagcttat cggatgccga tgatcgggga
     8641 gaaggcgccg gccttcaccg ccaagacgac gcaggggatc atcaacttcc ccgatgatta
     8701 caaggggaaa tgggtcattc tcttcagcca tccggccgat ttcacgccgg tctgcaccac
     8761 cgagttcatg accttcgcca gcctgcaggg cgatttcaag aaactgaact gcgagctgat
     8821 cggcctctcg atcgacagcg gctacagcca catcgcctgg ctgcggacga tcaaggagaa
     8881 gatccagttc cgcggcatga aggacgtcga ggtgaccttc ccggtcatcg aggacctcaa
     8941 gatggaagtg gccaggaagt tcggcatgct ccagcccagc gccagcgaca cccaggcggt
     9001 gcgggcggtc ttcttcatgg atccgaaggg gatcatcagg gcgatcatct actacccgct
     9061 ctccaacgga cgcaacttca aggagatcaa gcggctgctg atcgccatgc agacctcgga
     9121 ccgcttcggc gtggccacgc cggccgactg ggagccgggc gaggacgtga tcatcccgcc
     9181 gccgggctcc tgcggcctgg ccaaggaacg ggtcgaaaag gcaaagcagg atggttacta
     9241 ctgcctggac tggttcctct gcttcaagaa actgcccaag agccaactgg aagcgaagaa
     9301 gtaaggaaaa agaggcaaaa aaagaggcgg cggaggccgg caaaagcccc cgccgccttt
     9361 ctttttaacc ggtctttcag cgcgcgacgc cgctcaggat cagctggccg taaccgaacg
     9421 gactgcggcg cggcaggtgg ttcccggtcc aggagagaac cgcggccgga ccgtcgccgt
     9481 cctggtcaac cagttcgaca ttcaggccga gggcccgttc ctggcggccg gccgtgccga
     9541 tcagctccga gggcaggcgc agctcaaccc ggcggccctc atcggtgtca actaccaccg
     9601 cctcgcaccg gccgatctcc cgcccctggc catcgatcac cgccggctgc tgggggccgc
     9661 cggccagggc gacggttatg ctgaggtcat cggcatccgg aagcgggttg ccgaggtcgt
     9721 ccaacaggtc gcggtcaagc ttgacgacca ggccgtcgcc ggggcgtcgg acgccgtcga
     9781 gcacctcgaa ggcaaggtag gcgtcgcggc cgtcccagag gctcttgaag gagaccgagg
     9841 cgtcctcgag ctcgaagatc cggctgaagt tctggatgcc ctggagggcc ccgtaggagc
     9901 cgtcgagggc cgaccagcag tagatgaagg agggagcggc cttcacccag gccgcctcat
     9961 ccagccgtcc gtcgaccttg accgtgcccg cgccgaccaa tccggccact gtcgcccagg
    10021 aggcattgcg ctcgtactgg ccttggtcga tcggttcgct gaaaccggcc ccggccggga
    10081 agggctggcc ggagaccttc tccatccgcc ccttgatcat cttgaaggtg gccagctcca
    10141 ggccgggtcc ggccggcagg acgacccgcc gctccaggtc caccgggtag gccttgagag
    10201 accggatggc cgccagcagt ttatcgcggc ccaggccgtt atcctcgatg accagcagct
    10261 tgctgaccgg caggacgagc ttcgggctga acgaaaccgg gtttaactgg tagtcgtaaa
    10321 gcgcgacctg gcgggccggc aggtcaaggt tgacctcgat gttcccgtaa ctgccgtccg
    10381 ggcagagcag gacgcagaaa cggccgttct tccggtagag gccggcggcg tccaggccgt
    10441 aggcggtctc catcaggatc aggccgtcgg gccggtggcc gcgcaggaag ttgaccgcgt
    10501 tgatgaaatt ggcgctccag gggcgcaggg tattgtcgcc ggcaaagagg gcgaacggat
    10561 cgcggccgcc gtcgaacttg gcggtgtaga ggccgaagcg ttcggtgccc acgacggcgg
    10621 cctcgttgaa gacatcgtag atgaccgacc ggttaccgtt gagccagcgg ccgctccagt
    10681 agtcgcgccg cggctggaag gtctccgggt tgaggaccga accccagtcg ccggtggtcc
    10741 aacccacgcc gaccacccag tattgcttgc cggtaaaatc cttggcgtaa cggtcgtagt
    10801 tgaagaggtt gcgctcgaag ccgtggcggg agccgccgat ggcgtcgccg atatccagct
    10861 tgggattgag attgagcatc tccagcaggt gggtgatctg ggtgctgttc atgatcaccg
    10921 cgcccggtac gttcttgcgg atgatgcggt tggcgatttc gacgtaggga aggctctcct
    10981 tcggcccccg gtggtagctc tgctcgtcgg tcggctgcca ggccttgatg tagttggcgc
    11041 ggctggtgaa gcgctcccag aactcggccc aatcctggta ggggatattg ggcccgtcca
    11101 tgtgggcggc cggttcgacg ccgagaatgt tctcggtgaa cttgggtggg ttgaagtgtg
    11161 gccgccagaa ggagtactgg atgccgtatt tctcacccca catccggtaa taccggtcga
    11221 agaaacgcat ctcttcccga ctgggccaga tgtagtcgaa gtaggccatc cggtcgttga
    11281 gggagttgac ccagtcgaaa ccggcctgct tgaagatctt catgatctct tcgttggtgc
    11341 tgataatggc gcccagctgc ggaccgctcc cggcgaactg cttcgggtcc tcgatcacct
    11401 ggaaggggat cgcgtagccg agctccggcc gcttctccac ctcccagagc agcaggtagt
    11461 ttcccagccg gtcgaaggcc ggcttgaccg gcagggcgac cacgccgccg ggccgggatt
    11521 gaacgctctg gcggccggaa accagcacct ccaggtgggg gcccagaatc cggtaattaa
    11581 tcccgagccg ttccggacgc tgaccgacgg cctggctgac cgagatctcc agcggcaggt
    11641 tccggccccg gtagaagcgg ttgcccggaa cgccggtctg aatgccgacc tccaggccgt
    11701 ccaggggctg ataatcggag gcctgccggc cccgttccag ctgaagggca tccacccaga
    11761 ccagggcctg cgggtcgtcg gtcgtaaagc tgatggacgc ggcgggggta tactcaaccg
    11821 tgtagcgctg ccagtcggtg tggctgaccg tcttgccgtt gacgctcacc ggccggccgt
    11881 tggtgcgcag gtaccaggag agcgtgaggg ggccggtcac cgactttttg aactccgtgg
    11941 ccaggaactg gttgagataa aaaacattga aggccatccc gtaacggatg ctggccttac
    12001 tgccggtgta atcctcgagg aggttctggg gccgcggcac cagcttcagg gagtacctgc
    12061 cgtggtaggg acgctcggcg tccaggcaat cgttgtcgac attgttgccg gaggtcatgg
    12121 tatcgccggc gccggtgtag aagggcttga ccgaaaactc gaagctggag ttgcgcagca
    12181 ggttgcgagc cgggaagtag ggctcccgcg ccggcggcag gttctcgaag gcgaccgcgg
    12241 ccggggccga aggatacttg atccgttcgg cgctctggcg cgggtcggcg ccggcgaact
    12301 tcagttcgtt ccgaccccgg cgggtgtcct tgatgatctc aaccggctcg tcggtgatta
    12361 ccaggctgtc gaggatggcg taggggcgct ggtactccgg gtcgtcgccg atgccgcagt
    12421 tttccacggt caccgcctcg atctcaatga cctggcaggg caggccggtc ttgaggacgc
    12481 cggccagttc gtaccagccg tagccgctgc cggtgaagtc ctggacgaag ggagcggcgg
    12541 cgttggcggt gaacctgccg gccgggtcat agttgcccag cgaggccttc agcaggttcc
    12601 gggtgttggc cgagcgattc aggcagaccc gcaggaggag ccggtaggaa ccggccggaa
    12661 ccgggttgtc cagtttcagg tacatgcggg ccggatggtg ggtcacggcg aaggcgccgc
    12721 ccgaggcgtg gtagtggcgc ttgacgtacc agccgggcat gtccacccag ttgccgacat
    12781 agtccaggtt attgaacttc tccacctcgg tgaagagtga cagggcgccg gcctcgacag
    12841 ccagccccaa caacagaaaa aaagccagac aggcacctct caaaagtttc cgctgccgca
    12901 tacccacccc cttaaaattt cgagaattga agtttacttg ccggccgact ggaatatctt
    12961 ctttatctcc gcctcgggca gcgggcggtc gtaaatcctt atttcgtcca gggcgccgac
    13021 aagcccggtc aggtcgcggc cgatggtcag cggcgtctcg gtcgagatgt cggtcggcat
    13081 ctcgatcatg acgatgtcgc cgatcaggac gccgttgacg aagaggcgca gtccgcggtc
    13141 cctggacccg gtattgtaag tggcggccag gtgcacccag cggcccggct caacgtactt
    13201 ggccatggga accgagaccg tgtcccggcc gccgacgccg tcgacggtga agaggaagcc
    13261 ggccccttcc ttgatcttgg tcggaccaga cgagccgttg gtcccgaggt tgaagccccg
    13321 gaagggacgg taggcggtcg gaccgccgcc cttgccggtc gcctcggtgg ccgcgtcctt
    13381 cttcacgaaa ctggtccagc ggatggcgtc ggtgtccagg cgcagccaga gggaaacggt
    13441 caggccgttc ttgatggagc gcaggctcgg gctgtcggga accaggatgg cgcccttgcc
    13501 gtccaggacc agggccttcc cgaactggcc gtcggccaaa gccgcgcccg gaccgagttt
    13561 caggtgattg ttgttgccgg agaggtcgcg gaccacaccg tcaaccgcct cctccatgga
    13621 gtaatgcaga accaggcccg gctcggcggc gccagccgcc ggcgccaggg ccagcagcgc
    13681 cagggccaac agcggaatca atctcttcat ggctgacctc atttcgaagc ggccaggccg
    13741 gccaggaact cccgggccgc ggccagctgg ttgtcgggaa gcgtcaggta aacatacgcg
    13801 taaggctgga ccagggccag gtagcgcttg ccggcgcggt tcagggtgat gtcgttcccc
    13861 cagatgtcca gctggcgcag gttcaggccg gacgggacct cgacctcggc cgcggccggc
    13921 ttgccaggca tcacggccag gagctttccc ttcccctgac cgagcgagaa gacgaaacct
    13981 tcccagggca ggccaagctc accctcggag gcgccggcgg cggtcaggcc gttgaagcag
    14041 ccgagggcga cgatggaggg atccaggtga ccccagaggt cgaaggtcga ctggtagccg
    14101 ccgccgggca ggcggccgtg gtagatgatg accgccttga cccgcggccc gccgccgccg
    14161 gccggccgca ggttgcggac cagcgtatcg ttgaagcggc tggtgaagtc ggagaggcag
    14221 ctgtccacct ccacgccctc ggccgtccgg ccgagccggg aggcgaactt gccccagccg
    14281 ttgaaatggt cggagtgata attcatcagg accgagcagt catagaagaa gagcgggaag
    14341 tccggcccgg ccaggtcggc ggccttgacc gaataacgga cccggcccag ttcccggccc
    14401 aggtcgtaga ggccgccggt ggactccggc ccgccccgga cataggcgag ggccgcgttc
    14461 cgcgggtagg tggccaaaag aaccgccttg ggatcgaccg acttcagggc ccgggctccc
    14521 tcgaccatca gctgaaggta ggccggaccg ccgaagggca cctcgtcgat catctgccag
    14581 tacttgatgt agggcgagta atggcgggcc atggtctgca ggtactcccg gaaggccgag
    14641 atcttcaggt agcgcggccc gttggccgat tcgaggtcga tcgtctcgcc ggggatggac
    14701 ttctcggcct cggccatctt ggggaagacg tcaatgaaac cggcgtcgga gacgcagccg
    14761 acgaagcgca tccggtaccg gtcgcgggcg gccttcaggt agggcaggta ggcctcgaac
    14821 cagtactggt cgcgggcgta aatcttcttc cactcggtgg tcgcgccggc ggacgacagg
    14881 gtgttgttcc agtagaggcc gaggcgggtg gcccgctcca ggcccggcac gctcaactgc
    14941 aggtagaggc cgaagggatt ccagccgtcc gggtcgggcc tgggaaccac gtagaaggga
    15001 atctccatcc ggaggccgga gccgaaggtc ccgccgccgg cctcccgggc cagcacctgg
    15061 aagcggaaga cgcccttgcg ctccttcgga aaggcgtact tgaactcgga gacgccgtcc
    15121 ttcttgagca ggaaggaggc ccgggcggag gtcaccggaa actccctgcc gaagcagtcc
    15181 cagaggcggc cttccaggtc aaccttgacg tcgcgtccgg tcgggttgac cccgcgcagg
    15241 aggacctcca ccggaccgct gtcgtgaaag atgttggccg gcggcaggca gaccagccgc
    15301 agttccgggc cggccggcgc gtaagctccg gccgtctcgc ccgcttccag ctggagcgaa
    15361 tcgagccaga ggtggccggc gtgcagctgg ttcggatcgt aatcggagcc gctgaccgca
    15421 acggccagtt tctccggctt gtcgagcggc ccggaagcgg tcacccggcg ccagccctcc
    15481 tccggcagca cgacgacctg gatcttgatg gcgccgcagg ccaggttgaa attcagcttc
    15541 cggtcgctct tgacccaggc cgagagggtg taggtcccgg cccccggcac cggcaccgtg
    15601 aaggcgctgg tcagggaaag aaacgggctg aggctgtacc cggtcccggc gctccaggcg
    15661 gtcaggcgca gcgaggcggt gccgtcgtgg aagacctgga agtcgaggtc gctgtcgtcc
    15721 accagacggg ccgactggta ggaggccacc cagagccggt aggcgccggt ttcgaagccg
    15781 gagttgggga tgaggttgac ggcggcggcc ggccggccgc cgaagcagaa gagaaccgcg
    15841 gtcaggcaga gcaacttgcg aatcatgcgc acctccgggt aaggaacccc gcctgccggc
    15901 gggcggttat ggaaagccgg ttgtttcatc tctttagaag acggtcggat tcgtcttgtt
    15961 taaaagacgc ccttcgcccc tttgcgatcc gcgggagttt cggaccgggc gccgccggct
    16021 cccggcaagc cggaagatca ccgcggcccg tcgaagagat tatacttctt cgcgtccgga
    16081 ttgtcctggt gccacttccg gaaggcctcg ggaccctgca gtatgacatc cgcgggcgcc
    16141 accttcaggc cgggcaggag gggcgcggca tgcgttcccg gcttatcggg cagccggcgg
    16201 gccctggcca ggtcaaaggc cgctttcgtc gcgtacacgc gcccggagta ggccgggaca
    16261 acaacttcga tcgccgcggc gcccttcttg accgggtagg cctgctcggt ccgcacgtcc
    16321 accaggcccc cggcggcctt gagcgtcttc gaggccagcg gcagcttgac cgcccggggt
    16381 tggccggttc cgttgagcac gaccaccccg cgctcgtaag cgcgcaggta aacgccctcc
    16441 gcgacctcga cgataccgct gcggacctgc ttgaggcgaa gccagtttat cttgtccagc
    16501 ccggcggcgg gccaggccag gacatacctg ccgagccggg ccgccacgta cgcgaagaaa
    16561 gcccggtctt ccttgtcgcc gggtatgctc tcgggcacgc gcggcatgta aagcatgacc
    16621 ttcccgtgct cgaccgcctc ctggtagcgc cgggccgtct tcacgacata ggcgcagacc
    16681 tcggccgcgc tgccgtcggt cacctcgtgc atgaagccct cgatgagctg gccgtccacc
    16741 agccgccagg gcgccagtcc ctggctgttg gcccacatga cgcggtcggg gtggtagccc
    16801 ttgatcacct tgtagagttc ccagtagagg tcgtcgagga cctcggcgtt ggtccggtcc
    16861 ggaaaaaggt gccgatgctt gccgagttta tcgccgaagc agggcgggct tttctggtag
    16921 ttgtcgaaca tgatcccgtc gtatcccagc tccatgaggg agcggatggc cagtttccag
    16981 gcgtcctgga cttccttgag attgacgcag gtgatgtgcc ggcccgggta gaaaccccat
    17041 ttccagaccc agttcccgtc cgggtcgtag aggttccacc gcgcttcgcg gccatccttg
    17101 ttgacgtact tgccgggcca gccctgctga ccgggcagcc agcgtatctt gatggcgagc
    17161 tcgccccgcg gcgtgtcgcc ccgctcgtcc tccgtcctgg tgatgccgtg ctcccggacg
    17221 aacttcatgc ccagctcatg ctcaatgatg ccgaacatgg ggttgtcctg ataagccgag
    17281 gcgcgcgaac ccgcggcgtt ccaggcctga cgggtctcca ggcccgcgtt cggccagccg
    17341 ttcccggccg cgtaccagcc ggccttggcg gcctggaatc cgcccgaggg atggctgaac
    17401 tccagtttcc ccgccgtggc gggatccgac gagacggcgt cgaccacttc cagctcctgg
    17461 aaccacttcg gggccgggta gccgtattgc tcgtcatacc ggccaaggcc gggcgcccgg
    17521 gcaacctggc cggccgcgta cccgggcaaa accgacaaaa agaaaccggc caaaaacaag
    17581 gataaaatcc aggtacgcat gatgcgctgc atgtttatgc ctcctgaaag cgtgccggta
    17641 attgactacc caccgatttg ccagagaaat ccgtttttgg tttttcttgt ttaattataa
    17701 atatcaataa cgggataatt gaccggcggc ccgccccttg atggcggcca tccgggaggc
    17761 ggcccgctgg ccggcccgga ttttcttccg tatatatcat ctaaatttaa gccatgcgca
    17821 ccttctttgt caaattttta tatattatat aatctataat gaccgaaaaa aggcgggcgg
    17881 agatgcttct tgtccgtcat gcactcatgt cgtacgcccg tcatgcccac ggacttgtcc
    17941 gccgaagctc atttttcagc gtcggaggat agtaggcggg tatccaaaac gcctgtcatg
    18001 cccgcggata gttggcgggc atccaatcct taagaggttt taactgataa atgcctggat
    18061 tccctcctac tacgagaggg aatgacatgc tactgctgga ttccctccta ctgatgatta
    18121 tggaatacgt cttacttgga tccccgccta ccacatgcgg ggacaggcat gagggaatga
    18181 cccctgaagc cttcgacagg ctcaggcttg gcaaagccaa gtacaaaaaa ctcggcgtac
    18241 acttcagggc cttggattgg ttgttcagac gaatttcaaa aagtccgtca tgcccgtatg
    18301 ttgtagacgg gtatccattc cttaagaggt tttgagccaa aaacgacccc gggtaacatt
    18361 aaagaacgag gcacaaaaaa gaaggccggc gtcgggaaag accgccggcc gtctttacaa
    18421 agactctttt ccggatcgcc tcaggggttg ttgtaccagt agttggtatc cttgcactgg
    18481 tcggtggtca aaaactccac gtggccatcg tagaaaagaa agttggtgcc gccccggtgc
    18541 gtgggcacgc cggtgcacag gccgtagtaa ctcccgggag agtagtagga ggtaccgtta
    18601 ctgccaacgc tcgagaaggt gtattgatag ttgccgatgt tggtattgcc ggccagggtt
    18661 ccgcacatcg aaaagatgag ccgctccggc cgccgcacgt ccttggcccg gtagcgggtg
    18721 gtggccatct tgttcggcca gttcccgttg gcggggtagt caaagacgaa caggaacaac
    18781 ccgctgccgc ccgaggtgcc ggtgtagtag ccctcaccca tcgagccccg ggacgggtag
    18841 cagtaagccc ggcggtccat cgccttcgtg ccgaacttcg gacaccgcaa ggcctcctgg
    18901 ttcttgacgt aattctcatt atagagcatc tccgggtaac tgggccggga tacgcccacg
    18961 tcgccccacc aggaggccag caggtagtcg tcgttgtcgt tccagtagat ccgcatggtg
    19021 gtgccgagct gcttcaggtt gctcatgcac tgggccgcgt aggccttctc ccgggccgcg
    19081 ttcagggccg gcagcagcag ggcggccagg atggcgatga tggccaccac caccagcagt
    19141 tcaatcaggg taaagccc
//
LOCUS       DAOQYN010000010        18585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_3367_length_18585_cov_5.282623, whole genome shotgun sequence.
ACCESSION   DAOQYN010000010 DAOQYN010000000
VERSION     DAOQYN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18585
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3367_length_18585_cov_5.282623"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..730
                     /locus_tag="PKN80_00900"
     CDS             140..730
                     /locus_tag="PKN80_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNQ34606.1"
                     /translation="MRCRNVEKWIILELAGELGVRETAALERHLEGCRSCRELRSEYR
                     QLRTGLAEVEPVPVPFISRAWKVRAEGRLPRPGFLRPRVLASSLAMVMILAVVGFVYL
                     GQPDSPLWNESVLAGLEGSAPVTTGTIRINDSEPVPAVLARVEPPPALPRVVRRAAVS
                     LESTARAGAITIDYQPVLNNLDSEEKEMLLTILREI"
     gene            730..1386
                     /locus_tag="PKN80_00905"
     CDS             730..1386
                     /locus_tag="PKN80_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34607.1"
                     /translation="MEKQIGFAKRQVKGGNVYRYGLLFLAFFSILVTGSSAANPPGAD
                     NSADGGLGVKVQGSVERRDRDNSSFWEQLGTVRSEKLALYLELSSEDSKLVVPIIQAL
                     SRHRKEVQVERNAALDRLDGLLKQRAGEADLLRAVRDFQRQEVEAFQKEQRLTADLLR
                     ALTPEKQARYYLFNRYFSNQVRQGLYRMAEQRLKNRQDGPDAGPSRKVSTPPPPPGQP
                     "
     gene            complement(1307..1993)
                     /gene="cmk"
                     /locus_tag="PKN80_00910"
     CDS             complement(1307..1993)
                     /gene="cmk"
                     /locus_tag="PKN80_00910"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNQ34608.1"
                     /translation="MHIIAIDGPAGAGKSTLARQLARRLGFLYLDTGALYRALTWKAL
                     QEGVNLEDETALEKITRTARLDIRPAPEGVRVFVDGTEVTGEIRTPEVTRNTFYLARS
                     PRVRAALLPLQRQFGERNNLVVEGRDTTTVIFPRATLKIYLDASLKTRAGRRTLDLEK
                     IGARQPVPEVEREMAERDRQDLSRRVAPLVRAPDAHYLDTSGLTVDQEVAAVLKLFET
                     VRHQAHPGDS"
     gene            complement(2017..2574)
                     /gene="frr"
                     /locus_tag="PKN80_00915"
     CDS             complement(2017..2574)
                     /gene="frr"
                     /locus_tag="PKN80_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNQ34609.1"
                     /translation="MVARETIQRTQNEMHRVLDFVKKELSGLRIGRASATLLDGIQVN
                     AYNARMPINQVATVSTPQPNLIMVQPWDKNLAGEIGKAILASNLGLNPVTDATGVRIP
                     IPPLSEERRREVVKVAHKIAEQGRVEIREMRRKANEEIKKAEKEKRVSEDDMHHVIND
                     IQKQTDNAIREVDNLLAAKEKEIME"
     gene            complement(2605..3315)
                     /gene="pyrH"
                     /locus_tag="PKN80_00920"
     CDS             complement(2605..3315)
                     /gene="pyrH"
                     /locus_tag="PKN80_00920"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNQ34610.1"
                     /translation="MAKKYRRVVVKVSGEAFSGEDGFGIEPEPLKRLVGELRSAAELD
                     IQIAVVIGAGNLFRGAVVSREGIDKTTADYMGMVATVINGLALQSALEKQGVETRLQT
                     ALEMRQLAEPYIRRRAIRHLEKGRLVIFAGGTGSPYFTTDTAAALRAIEIGAQLILKG
                     TKVDGVYSADPKRIRDARRYQRLTFDTVLQKNLAIMDATAIALCRENTIPVVVFNLFG
                     KGNLRQILAGGKLGTIIS"
     gene            complement(3358..4848)
                     /gene="metG"
                     /locus_tag="PKN80_00925"
     CDS             complement(3358..4848)
                     /gene="metG"
                     /locus_tag="PKN80_00925"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNQ34611.1"
                     /translation="MSKYYLTTPIYYVNAQPHIGHVYTTVAADALARWFRLQGREVFF
                     LTGTDEHGSKIENAAREAGISPGEMADRMSKNFRDLWVAMEIKPDRFIRTTEPEHQRV
                     VQRVFLELSARGDIYPGVYEGYYCFACEAFLTPSQGSLCPDCGRETEKISEPTYFFRL
                     SRYQEPIRAYFQAHPEMIQPERAREEVSNLLNQPLPDVSCTRRCAWGIPLPAEMAADP
                     ELTIYVWFDALLNYLSGVDYGQEDGRFKKTWPPDIQLLGKDIIRFHYIIWPALLLALG
                     LPLPEKLFAHGWWLSGGEKMSKSRGNVVEPMEFARTYGVDALRYFLLAEVPFGLDGDF
                     NEAAFRKRYNSDLANDLGNLVHRFAHLIRTRHQGRVPEAGDGGRLAEKVLAALPQLDE
                     RMAAVDFFGFLETARTVVTAANHYLDETAPWRPENNSGPVLYNLAETARLLSLCLEPV
                     MPRTSGLIRSRLGLEKLPADSNGLRWGLTPAGAAVGEGAPLFPRLK"
     gene            complement(4841..5545)
                     /gene="ricT"
                     /locus_tag="PKN80_00930"
     CDS             complement(4841..5545)
                     /gene="ricT"
                     /locus_tag="PKN80_00930"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNQ34612.1"
                     /translation="MKFAELRIRRLDKVEPFSAPEELEFAPGDYVIIESSKSGLDYGI
                     VLSILPGPPAGRKVDDLSSIVRKVTPEDIEQIEENYEHDEEAFRTCQEKILEKKLANM
                     KLLDAEYSFNRSVVTFYYYAEERIDFRELVRELAKTFNCRIEMRQVGRRDEARMLGGF
                     GVCGRRLCCSLFLREFKPVTMRMVKDQNLPLDTSKVSGLCSRLMCCLAYEHHSCPRQG
                     NNPEPEEAEEKDAPNE"
     gene            complement(5542..7350)
                     /locus_tag="PKN80_00935"
     CDS             complement(5542..7350)
                     /locus_tag="PKN80_00935"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNQ34613.1"
                     /translation="MKAGKNRFNPAILPLTGFAVYIFLRPLLDGLTHYNLQLLFQLAV
                     TAAAAVYAGLILLGRARYRPTGLEAAALLFLAVNAISAAVSTSPYRSTRLFLEHLHYF
                     LLFFLLVQMLAAAGERGERFSRVLRAVILASGILVCLYALEQYFWGFRETLGYLKESN
                     LLATTNPDLLARLYSRRVFSTFVYPNILAGYLIALLPVAISLGSEAGADRRTLAALLS
                     LLLLTTLGLTRSTGAMAALGITGLLWLAAAWSGRRARARTGTPDRLQPAWLVPVLTLA
                     GGLIGLWLILRAAGLWTPLLGDKAITLRDRLAYWSSSWRMFLREPVLGFGPGSFGVHY
                     TRFKIMRALETQHSHSLFFEMLAENGLLGTGAFLAFWALLLRRIAPRMAGGGAAAGLG
                     WGAIALFVHAQLDFDFSDPSLASFLFILPALIPVRSGRPDGRPDRAGSSNRPWLTRLG
                     AGLIILIMVAAGIRQARLYRADRIFQNGLTELSQGTGRPGLESLHRAAGMEPDNPHYW
                     AQLADLYFQAGRFGNSTVLLEKAVENYRRAVRVEPFASGYHYRLGLASEDLAGRTGQA
                     VWKRGAATAFRRAAELYPDNPNYRQKNLPYHTPGNE"
     gene            complement(7347..8237)
                     /locus_tag="PKN80_00940"
     CDS             complement(7347..8237)
                     /locus_tag="PKN80_00940"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNQ34614.1"
                     /translation="MSKTPDLALRFAGIKLRNPLVLLSGTAGAGREIKKLVELGRFGA
                     LCTKTVTFQPRPGNPPPRIAETPSGLVNSIGLENPGFARFLEETAPGLRSLPLPVIAS
                     IAGNGPELSRMAEALTGAGPAGLEINLSCPNVGGAFNPTAPTEIERLLREVRRATGLP
                     LLAKLPPDFYRIGDLARAAETAGCDGLTVANTLPAMVFDLKLRKPRLGGITGGLSGPA
                     VLPVSLYLVHRARQAVGLPIIGSGGVHDADGAVSMLLAGAQAIGLGTVNFFRPLAYRE
                     ILQALAEFRTAGPGRRKPTP"
     gene            complement(8221..9012)
                     /locus_tag="PKN80_00945"
     CDS             complement(8221..9012)
                     /locus_tag="PKN80_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNQ34615.1"
                     /translation="MRDYDTLILANRRPAGVDDCRRLLLRRPAGFTPRPGQFIQIEVN
                     RAGTDPLLRRPFSIADLQPETVTVIYRVQGRGTARLAERRRGEAIRFLGPLGKDYPAA
                     DGPCLLVAGGMGAAGLLFLARRLAELGRAGSATLLLGCRDRNGLFFRTAFQKLGVRTV
                     CATEDGSAGRAGLVTDLLRNELNRDRKPVVYACGPAAMLAGVHEATRGTGIRAYVSLE
                     TVMACGSGLCRGCAVPTRAGYRMACTDGPVFAAEEIDWKAYVENA"
     gene            complement(9018..9956)
                     /locus_tag="PKN80_00950"
     CDS             complement(9018..9956)
                     /locus_tag="PKN80_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HNQ34616.1"
                     /translation="MDIYTLTCNPSQDFYLEVIQIVPEDINRSRAARRDLGGKGINVS
                     RGLKSLGSRTRALGLAAGDTGRWILNQLKREGIPGRFFRAEGENRVILNIREGRTGRI
                     YRFNEPGPRLDRKRLRAFWNFVCDNSCPRGSFFAVCGSAPPGFPADFYGRLITRLKER
                     GIRTLLDCDHRLLAAGTQAGPEILKVNLFELARLTGTRHPAGQAEVIRGVRRLLGCGI
                     KNVVVTLGAEGALALDEKDGWWARPPRVKARSTIGAGDALSAGLLHGLSESKGWAEAL
                     RLGVAAGTAAVRYPGTTFANPEEIRRLLRRVKIQPV"
     gene            complement(9962..10129)
                     /gene="rpsU"
                     /locus_tag="PKN80_00955"
     CDS             complement(9962..10129)
                     /gene="rpsU"
                     /locus_tag="PKN80_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNQ34617.1"
                     /translation="MRHRTRQSDDFEKIFKRFKREVEREKILQEIKSREYYEKPSRQR
                     RLKKLRKKRSR"
     gene            complement(10184..10663)
                     /locus_tag="PKN80_00960"
     CDS             complement(10184..10663)
                     /locus_tag="PKN80_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HNQ34618.1"
                     /translation="MAHSRSKVKEKSIPKKSPKSGPGKGIRKVKPAPARKVAAKASAK
                     ATARTTAKKAPPKKKAVVRPTPKKKAPVKPAVKSPTRKAAPTKPAAGKKTAAKPAGKT
                     PVRKTAPVKPVAVKIPSKPVELRSNIRCRSCFMVDGYYENEESCHYCKAKIYAVDAV"
     gene            10859..12856
                     /locus_tag="PKN80_00965"
     CDS             10859..12856
                     /locus_tag="PKN80_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34619.1"
                     /translation="MDRIRALIRLPYLLLALLLSSRIGWTAEPPAVIWRAGSSEVRLA
                     EGRVEEFILEGGVVFDDGARRVQADRARYFPERREALLEGGVVINLPAGEIRAESARY
                     ILDQSVGESGPAVFAAPPWYGRVERTRILSPDEIQLENGYLTTCDLVDPHYRISLDSA
                     RIKPDKWVKMRSATFRVGRVPVFYLPAVSQRLDSGSPFAFYADPAISSRDGFQLLTTV
                     SYRTEAARYDFDLDYRGHKGPGFGFGFEQVQPGRRTEFKTYYIHDLDRHRDGYRGELW
                     HRSAWPRSEGEDTLRLEAHTFSEAGFLKDFFWRESTTDRDRESRLAYAVNRSGYYLGA
                     AVEGELDDFYNRTEYLPVLRLYRPARSLGPGYWQGDLEAAALARDFGGRRRETGRVHL
                     VETLSGFRPLLGGTWRPFASAGATLYSGTTEGSENRVRSLFQSGFNLDWKFGRRYGPE
                     EAEEGWTHYLEPRISVLSRDIGERPGRFYQYDLIDSLDSDQVVSLQLTNRLLAGPSGE
                     GREALRFDLKGDYSLKRGRPGDLYSLINYSPDPAFSFYSEGDCDLDRLAWRSLSSSMV
                     WHRPPFDFGLSHSYQRNESELLTPFVSLPLGRRWRLETRVAFNLKDDGLESREVSLWK
                     DMHCWEGRFGVYHDRKETEVYLLFYPKIFPGRSLKIKSLID"
     gene            12919..12990
                     /locus_tag="PKN80_00970"
     tRNA            12919..12990
                     /locus_tag="PKN80_00970"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12951..12953,aa:Met,seq:cat)
     gene            13011..14165
                     /locus_tag="PKN80_00975"
     CDS             13011..14165
                     /locus_tag="PKN80_00975"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNQ34620.1"
                     /translation="MPLKILIAPDSFKGSLTAAAAAAAIRTGFFRVWPRAEYRLLPLA
                     DGGEGTLAALLAARGGRRFRLEVRGPRGRPVRAVCGQLPDASGVVEIASAGGLPLLEA
                     SERNPRLASSYGNGQVIRFLLGRPVSGLLIGLGGSATVDGGLGMLTALGARFLDVRGR
                     VLSGIGGDLERVRAIDLSNLDCRLQGLPVTALCDVDNPLLGVRGAARVFGPQKGAGPA
                     MVERLEAGLANFAERVRSELGFDGARRPGAGAAGGLGFGLAACLGARLVNGADFIMKS
                     LGLERFLAWSDLAVSGEGRVDGQVKFGKALHRVGGLARQVGRPLLVFCGERDLADRER
                     RRLGFTAVFAIEPGPVTGAEAMARARIYLADTAAETARLMAGTPGRFARD"
     gene            14188..16020
                     /gene="dnaX"
                     /locus_tag="PKN80_00980"
     CDS             14188..16020
                     /gene="dnaX"
                     /locus_tag="PKN80_00980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNQ34621.1"
                     /translation="MSYEALALKYRPRTFKEIRGQEEITATLRRSIGEGRIYPAYLLS
                     GPRGVGKTSTARILTRSLNCVKGPAEEPCNKCETCLEILNGNSLDFFEIDGASNRGIE
                     EVRTINENTRFLPVKSRYKVYLIDEVHMLTDQAFNALLKTLEEPPEHVRFIFATTHPD
                     KLPGTVISRCQRYDFHLIPGPVLVQHLQEIAGKESIDIEPAALVRISQAAAGSFRDGI
                     AVLDQLVSFRYGQQITAVDVEKLLSLVPEEEYLDVFQALVTGDGRRCLEKVGALSQAG
                     YDLYKFTSGLLSFIRYLLLLKAGAGDLIQDVVTPETERALVEWAGRLDIETLLALLEG
                     LADEREKLRFEDLVRVRLEVSLLKLAGRAGGRTPVIDSGGGRSDSARPAVRLKPVSPA
                     PEPVKPPASEPPKLPPTAPSVAPASELSEASPAAIDAPPPLGTGAAASDAALAGAEAS
                     AVLDPAACWPGFLNELLRREPFFHAPLQQGGLQEIRTAEKPPVLVVGINNLFEQDLLK
                     KNSRRLSEIWQECYGMSVRFEAVRLPEKGIGRIPGPPPGLKETASKKVRPPAPAAPPA
                     GETTPAREIPATGTGSASLSPDEEAMVRRIQGIMGGEVKEIFKE"
     gene            16042..16353
                     /locus_tag="PKN80_00985"
     CDS             16042..16353
                     /locus_tag="PKN80_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNQ34622.1"
                     /translation="MANFMQNLKELNQLRQTAKVIEKKLREQKREFSSRDGRIKGVIN
                     GKLEILSLEVAPELLTAEGKAALERLLMSTINNAVSDMQSQVSKVVSSQMGINLPGLG
                     F"
     gene            16384..17262
                     /locus_tag="PKN80_00990"
     CDS             16384..17262
                     /locus_tag="PKN80_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNQ34623.1"
                     /translation="MFLFFVYNFSAFLALHLPEPAARTLARVTSALFYLVTPGMKRNA
                     RRNFRVMLSSGGREPTAGELERLVWRAYLNFGLYLYEFFLIPRIDGEFLNRRLEVRGL
                     EHLDSAIGGGRGVISITGHLGNWELAGILTSMMGYPVTAVALDHRSPSMNKFFLHRRR
                     MKGVKIVAVGGRTLALIRALKKSGIVALLADRVFSRSRLRMDFFGRSTYLPAGPAELA
                     VLTGSPIVPGFLVRRGRRYLLSFEPPIHPDLASGRSSAVRTLARHVIDVLERYIRTYP
                     EQWLVFEYLWNKETAF"
     gene            17262..18305
                     /gene="vorB"
                     /locus_tag="PKN80_00995"
     CDS             17262..18305
                     /gene="vorB"
                     /locus_tag="PKN80_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNQ34624.1"
                     /translation="MSGNEVAGEAAVAAGCRFYAGYPITPQNQLTEYMSRRVPEVGGV
                     FIQAESELAAINMVFGAAAAGARAMTSSSSPGISLKQEGISYLAGCELPAVIVNMNRA
                     GPGLGDVTPSQADYFQSTRGGGHGDYRTITLGPQNLQELYDLTYLAFELADRYRNPAL
                     ILGDGILAQVMEPVRLKPLRRVRPAPAWALTGCGDGPPRKILSLFLKPGVAEAHNWRL
                     AAKYRRIEVREQRWDEFQVEDAKLVVAAFGSSARIARASVQAAREEGLAVGLFRPVSL
                     WPFPYQPLRRLAEGRRRFLVVEMNLGQMVEDVRLAVENRGPVSFYGRPGGGVPTPEEI
                     LKVIRKTYRGAER"
     gene            18302..>18585
                     /locus_tag="PKN80_01000"
     CDS             18302..>18585
                     /locus_tag="PKN80_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34625.1"
                     /translation="MSQTYLFKRTEGLQDAPTSFCPGCGHGILLRLVAELVWELGLRG
                     RTVGVAPVGCAVTAYDYWNFDVTEAAHGRAPAVATGIKRVHPDRLVFTYPG"
BASE COUNT         3032 a         5822 c         6261 g         3470 t
ORIGIN      
        1 cttcggactg aagaatctgc gccggatcat caaggagaag gatccggtcg gcgtgctctt
       61 caagcagtca tgattttcag gcggggtgtt aaggccggcc tggttggggc gagtcagtca
      121 gtttaaagag aggcggaaaa tgagatgccg taatgttgag aagtggatca ttctggaact
      181 ggccggggag ctgggagtcc gggagaccgc ggcgctggaa aggcacctgg agggttgccg
      241 ttcctgccgc gaactccggt cggaataccg tcagttgaga acgggactgg cggaggtgga
      301 accggttccg gtccccttca tatccagggc ctggaaggtt cgggcggaag gccggctgcc
      361 gcggcccggt ttcctgcggc cgcgcgtcct ggcttcttcc ctggccatgg tgatgattct
      421 cgccgtggtg ggattcgtct acctgggcca gccggattcg cccctctgga acgagtcggt
      481 cctggccggc ctggaagggt cggcgccggt aaccaccgga acgatcagga tcaatgattc
      541 cgaaccggtg ccggcggtgc tggcccgggt tgaacccccg cccgctctcc cccgggtggt
      601 tcgccgggcc gcggtttccc tggaatcgac cgcgcgggcc ggggcgatca ctattgacta
      661 ccagccggtc ctgaacaacc tggacagcga agagaaggag atgctgctga ccatcctgcg
      721 ggagatttga tggaaaaaca gatcgggttc gcgaaaaggc aggttaaagg agggaacgtg
      781 taccggtacg ggctattatt cctggcgttc ttttcaatcc tggttacggg ttcgtcggcg
      841 gccaacccgc ccggcgcgga taattcggct gacggcggcc tgggcgtgaa ggttcagggt
      901 tccgtcgaac ggcgcgaccg ggacaacagt tccttctggg agcaactggg gacggtccgt
      961 tccgaaaagt tggccctcta tctcgagctg agctcggagg attcgaagct ggtcgtgccg
     1021 atcatccagg ccttgagccg gcaccggaag gaggtccagg ttgaacgcaa tgccgccctg
     1081 gaccggctgg acggacttct gaagcagagg gcgggcgagg cggatttgct gcgggcggtc
     1141 cgggattttc agaggcagga agtcgaggca ttccagaagg agcagcggtt gaccgccgac
     1201 ctgctgaggg cgcttacgcc cgagaaacag gcccgctact atctcttcaa ccgttacttt
     1261 tcaaaccagg tccggcaggg tttgtaccgg atggccgaac agcgtctcaa gaatcgccag
     1321 gatgggcctg atgccggacc gtctcgaaaa gtttcaacac cgccgccacc tcctggtcaa
     1381 ccgtaagccc ggaggtatcc aggtaatggg cgtcgggggc gcgcaccagc ggcgccaccc
     1441 ggcgtgacag gtcctggcgg tcccgctcgg ccatttcccg ctcgacttcc ggaaccggct
     1501 ggcgggcgcc tatcttttcg agatcaagcg ttcgccttcc cgccctcgtc ttcagactgg
     1561 catccaggta tatcttgaga gtggcccgcg gaaagatgac cgtggtcgta tcccgcccct
     1621 ccaccaccag gttgttccgt tccccgaatt ggcgctggag cggcagcagg gcggcccgga
     1681 ccctcggtga gcgggccagg tagaaggtgt tccgggttac ttccggggtc cggatctcgc
     1741 cggtcacctc ggttccgtcg acgaagaccc ggacgccttc cggggcgggg cggatgtcca
     1801 gccgggccgt ccgggtgatc ttctccaggg ccgtttcgtc ttccaggttg actccctcct
     1861 gcaacgcctt ccaggtaagc gcccggtaga gggcgccggt atccagataa aggaaaccga
     1921 gccggcgggc cagctgtctg gccagggtac tcttgccggc gccggccgga ccatcgatgg
     1981 caatgatgtg catcggggat ggatggaggc cgccggctac tccatgatct ccttctcttt
     2041 ggccgccagc aggttgtcga cctcccggat ggcgttgtcg gtctgcttct ggatgtcgtt
     2101 gatgacgtgg tgcatgtcat cctcggagac acgcttctct ttttcggcct tcttgatctc
     2161 ctcatttgcc ttgcggcgca tctcgcggat ttcgacccga ccctgctcgg cgatcttgtg
     2221 ggcgaccttg accacctccc ggcgccgctc ctcgctgagt ggcgggatgg gaatgcgcac
     2281 gccggtggca tcggtcaccg ggttcaggcc caggttggag gccaggatcg ccttgccgat
     2341 ttcgccggcc aggttcttgt cccagggctg gaccatgatc aggttgggct gcggcgtgct
     2401 gacggtggcc acctggttga taggcatccg ggcgttgtag gcattcacct ggatgccgtc
     2461 cagcagggtg gcactggcgc gcccgatccg gagcccggaa agttccttct ttacgaagtc
     2521 gaggacccgg tgcatctcgt tctgggtcct ctgaatagtt tctcttgcaa ccatgccgcc
     2581 tccttgaaag tatggaaatc ctgctcagct gataatcgtg cccagttttc cgccggccag
     2641 tatctgcctc aggttgccct tgccgaagag attgaagacg accaccggga tggtattctc
     2701 ccggcagagg gcgatggcgg tggcgtccat gatggcgagg tttttctgga gcacggtgtc
     2761 gaaagtgagc cgctggtagc gccgggcgtc ccggatgcgc ttgggatcgg ccgagtagac
     2821 cccgtcgacc ttggttccct tcagaataag ctgggcgccg atctcgatgg cgcgcagggc
     2881 ggcggcggtg tcggtggtga agtagggact gccggtgccg ccggcaaaga tgaccagccg
     2941 tcccttttcc aggtggcgga tggcccgccg ccgtatgtaa ggttcggcca gctggcgcat
     3001 ttcgagggcg gtctgaaggc gggtctcaac gccctgtttt tccagggccg actgcagggc
     3061 cagcccgttg atgaccgtgg ccaccattcc catgtaatcg gcggtggtct tgtcaatgcc
     3121 ctcgcggctg acgaccgcgc cgcggaaaag attgcccgcc ccgatcacca ccgcgatctg
     3181 gatgtccagt tccgccgcgg aacgcagttc accgaccagc cgttttaagg gttcgggttc
     3241 gattccaaat ccgtcctccc ccgaaaaggc ctccccggag accttcacca ccacccgccg
     3301 gtacttttta gccatgttct cctttgatta ctcgccgccc atccctggct gagatcgcta
     3361 tttcaaacgc ggaaagagtg gcgctccttc tccaacggcc gccccggccg gcgtgagccc
     3421 ccagcgcagc ccgttgctgt cggccggcag tttttcgagt cccagcctgg accggatgag
     3481 gccgctggtc cggggcatga ccggttccag gcagaggctg agcagccggg cggtctcagc
     3541 caggttgtaa agcaccgggc cggaattgtt ctccggccgc cagggtgcgg tttcgtccag
     3601 gtaatggttg gccgccgtca ccacggtgcg ggccgtttcc aggaagccga agaaatcgac
     3661 cgcggccatc ctttcgtcga gctgcgggag cgcggccagg accttttcag ccagccggcc
     3721 gccgtcgccg gcctccggca cccggccctg gtgccgggtt ctgatcaggt gggcgaaccg
     3781 gtgtaccagg ttgcccaggt cattggccag gtcgctgttg tagcgtttcc ggaaggcggc
     3841 ttcgttgaaa tccccgtcca gtccgaaggg gacctcggcc agcaggaaat agcgcagggc
     3901 gtcaactccg taagtccggg cgaattccat cggctcgacc acgttgccgc gcgacttgct
     3961 catcttctcg ccgccggaga gccaccagcc gtgggcgaag agcttctccg gcaagggcag
     4021 gcccagggcc agcagcaggg ccggccagat tatgtagtgg aaacggatga tatccttgcc
     4081 caggagctgg atgtcgggcg gccaggtttt tttaaaacgg ccgtcctcct gcccgtagtc
     4141 gactcccgag agatagttga gcagggcatc gaaccagaca tagatggtca attccggatc
     4201 ggccgccatc tcggctggca gcgggatgcc ccaggcgcag cggcgggtgc aggaaacgtc
     4261 gggcagcggc tggttgagca ggttggacac ctcctcccgc gcccgttccg gctggatcat
     4321 ctccgggtgg gcctgaaagt aggcccggat cggttcctgg taacgggaga ggcggaagaa
     4381 gtaagtgggt tcggagatct tttcggtctc ccggccgcag tccgggcata ggctgccctg
     4441 cgacggcgtc aggaaggcct cgcacgcgaa acaatagtaa ccttcgtaga cgcccgggta
     4501 gatgtcgccc cgggccgaca gttccaggaa gacccgctga accacccgct ggtgctccgg
     4561 ttcggtggtc cggatgaagc ggtccggttt gatctccatc gcgacccaga ggtcgcggaa
     4621 gtttttggac atgcggtcgg ccatctcccc gggactgatg cccgcctccc gggccgcgtt
     4681 ctcgatcttg ctgccgtgct cgtcggtgcc ggtcaggaag aagacctccc ggccctggag
     4741 gcggaaccat cgggccagcg cgtcggcggc caccgtggta tagacgtgtc caatatgtgg
     4801 ctgggcattg acatagtaga tcggcgtggt caggtagtac ttactcattg ggcgcgtcct
     4861 tttcctcggc ttcttccggt tcgggattgt ttccctggcg cgggcaggaa tgatgttcgt
     4921 aagccaggca gcacatcagc cggctgcaga ggcccgagac cttgctggta tccaggggca
     4981 ggttttggtc cttcaccatg cgcatggtga ccggtttgaa ttcccgcagg aagagcgagc
     5041 agcagagacg gcggccgcag actccgaaac cgcccagcat tcgggcttcg tcccgtcggc
     5101 cgacctggcg catctcgatc cggcagttga aggttttggc cagttcccgg accagctcgc
     5161 ggaaatcgat gcgttcctcg gcatagtagt aaaaggtaac caccgagcgg ttgaacgagt
     5221 actcggcgtc gaggagtttc atgttggcca gctttttttc caggatcttc tcctggcagg
     5281 tgcggaaggc ttcctcgtcg tgttcgtaat tctcctcgat ctgttcgatg tcctcgggcg
     5341 tgaccttgcg cacgatggac gagaggtcgt ctaccttccg gccggccggt ggtcccggca
     5401 ggatggagag gacgatgccg taatcgaggc cgctcttgga tgattcgatg attacgtaat
     5461 cgcccggcgc gaattccagt tcttcggggg cgctgaaggg ttcgaccttg tcaagacgcc
     5521 tgattcgcag ttcggcaaat ttcattcgtt ccccggggta tggtacggaa gatttttttg
     5581 acggtaattc ggattgtccg ggtagagttc ggccgcccgg cggaaggccg tcgccgcccc
     5641 ccgtttccag accgcctggc cggttcggcc ggccaggtcc tcgctggcca gtccgagccg
     5701 gtagtggtaa ccggaggcga agggttcgac ccggaccgcg cgccggtagt tttcaacggc
     5761 cttctccagc aggaccgtcg agttgccgaa cctcccggcc tggaagtaaa gatcggccag
     5821 ttgcgcccag taatggggat tatccggttc catcccggcc gcccggtgca ggctttccag
     5881 cccgggccgg ccggtgccct gggagagttc cgtgagaccg ttctggaaga tgcggtcggc
     5941 ccggtagaga cgcgcctgtc ggatgccggc ggccaccatt attaatatta taagcccggc
     6001 tcccagccgg gtcaaccacg gccggttgga tgaccccgcc cggtccggcc gcccatccgg
     6061 gcggccggac cggaccggga tgagagccgg cagtatgaag aggaaggagg ccagggaggg
     6121 gtcggagaag tcgaaatcga gctgggcgtg gacgaagagc gcgatcgcgc cccagcccag
     6181 accggccgcc gcgccgccgc cggccatgcg cggagcgatc cggcgcagca gcaacgccca
     6241 gaaggccagg aaggcgccgg ttcccagcag tccgttttcg gccagcattt cgaagaagag
     6301 gctgtggctg tgctgggtct ccagggcccg catgatcttg aagcgggtgt agtgaacgcc
     6361 gaaactgccc gggccgaagc cgaggaccgg ttcccggagg aacatccgcc aggagctgga
     6421 ccagtaagcc agccggtcgc gcagcgtgat cgccttgtcg cccagcagcg gcgtccagag
     6481 cccggcggcc cgcaggatca gccagagtcc gatcaggccc ccggccaggg tcaggaccgg
     6541 gaccagccag gccggctgga gccggtcggg cgttccggtc cgggcgcggg cccgccgtcc
     6601 ggaccaggcg gcggccagcc agaggagccc ggtaatcccc agggcggcca tggcgccggt
     6661 ggaacgggtc aggcccagcg tggtcagcag cagcagcgac agcagggccg ccagcgtacg
     6721 ccggtcagcc ccggcctccg aacccagcga gatcgccacc ggcaggagcg cgatcaggta
     6781 tccggccagg atattgggat agacgaaggt cgagaagacg cggcgggaat aaaggcgggc
     6841 cagtaggtcg ggattggtgg tggccagcag gttcgactcc ttaaggtacc ccagggtttc
     6901 ccgaaagccc cagaagtact gttcgagcgc atacaggcag accaggattc cggaggccag
     6961 gatgaccgcg cggagtacgc ggctgaatcg ttcgccccgc tccccggcgg cggccagcat
     7021 ttgcaccaac aggaagaaga ggaggaagta atgcaggtgt tccaggaaga gacgggtgga
     7081 acgataaggc gaggtcgaga ccgcggcgct gatcgcgttg accgccagga agagcagggc
     7141 cgccgcctcc agaccggtcg gccggtatcg ggcccggccc agcaggatca gcccggcgta
     7201 aaccgcggcg gcggccgtca ccgccagctg gaagagaagc tgcaggttgt agtgggtcag
     7261 gccgtccaga aggggacgca ggaagatata gactgcgaac ccggttaacg gcaggatagc
     7321 cgggttgaac cggttcttcc cggctttcat ggcgtaggtt tccggcggcc ggggccggcg
     7381 gtccggaatt cggccagggc ctggagtatt tcccggtagg ccagcggccg gaagaagttg
     7441 acggtgccca gcccgatcgc ctgggcgccg gccaggagca ttgagaccgc cccgtcggcg
     7501 tcgtggacgc cgcccgatcc gatgatcggc aggccgacgg cctggcgggc ccggtggacc
     7561 aggtagaggg agaccggcag gaccgccgga ccggagaggc ctccggttat cccgcccagc
     7621 cggggttttc gcagtttgag gtcgaagacc atggccggca gggtgttggc cacggtcagg
     7681 ccgtcgcagc cggccgtttc ggccgcccgg gccaggtctc cgatccggta gaagtcgggc
     7741 ggcagcttgg ccagcagagg caggccggtg gcgcgcctga cttcccgcag cagccgttcg
     7801 atttcggtcg gcgcggtggg gttgaaggcc ccgcccacgt tcggacagga gaggttgatc
     7861 tccaggccgg ccggcccggc tccggtgagc gcttcggcca tccgggagag ttccggtccg
     7921 ttgccggcga tgctggcgat caccggcagg ggcagggagc gcagtccggg cgccgtttcc
     7981 tcgaggaaac gggcgaaccc ggggttctcc aggccgatgg aattgaccag gcccgacggc
     8041 gtctccgcaa tgcggggggg cggattgccc ggccggggct ggaaggtcac ggtcttggtg
     8101 cagagggctc cgaagcggcc cagttcaacc agttttttga tttcacggcc ggcgccggcg
     8161 gtcccggaaa ggagcaccag cggattgcgc agcttgatgc cggcgaagcg cagggccaga
     8221 tcaggcgttt tcgacatagg ctttccagtc aatctcttcg gccgcgaaga ctggtccgtc
     8281 ggtgcaggcc atccggtagc cggcccgggt cggtaccgcg cacccgcggc aaaggccgga
     8341 accgcaggcc atgactgttt caagcgagac gtatgcccga ataccggtgc cgcgggtggc
     8401 ttcgtgcacc ccggccagca tggcggccgg cccgcaggcg taaacgaccg gtttccggtc
     8461 ccggttcaat tcgttccgga gcaggtcggt taccaggccg gcccggccgg ctgatccgtc
     8521 ctcggtggcg cagacggtcc gtacccccag tttttgaaag gcggtccgga agaagagtcc
     8581 gttccggtcc cggcagccca gcagcagggt ggccgaaccg gcccgtccca gttcggccag
     8641 gcgccgggcc aggaagagga gcccggcggc gcccatgccg ccggccacca gcaggcatgg
     8701 cccgtccgcc gccgggtagt ccttgcccag cggccccagg aaccggattg cttcgccgcg
     8761 gcgccgttcg gccagccggg ccgtaccgcg cccctggacc cggtagatga cggttaccgt
     8821 ttccggttgc aggtcggcga ttgaaaacgg ccttcgcagg agcggatcgg taccggcgcg
     8881 gttgacttcg atctggatga attgtccggg gcggggggta aacccggccg gccggcgcag
     8941 cagcaggcgg cggcaatcgt cgaccccggc cggccgccgg ttggccagga tcagggtatc
     9001 gtaatcgcgc atactcttca gaccggttga attttaaccc ggcggaggag ccgtcggatc
     9061 tcttccgggt tggcgaaggt ggtcccggga taccggaccg ccgcggttcc ggcggccacg
     9121 ccgagtcgga gcgcttcggc ccagcccttc gattcggaga gcccgtgcag gagtccggcg
     9181 ctaagcgcgt cgccggcgcc gatggtgctg cgcgccttga cccggggcgg tcgggcccac
     9241 cacccgtcct tttcatccag agccagggct ccctcggcac cgagggtcac caccacgttt
     9301 ttgatgccgc aaccgaggag gcgccggacg ccgcgaatga cttccgcctg gccggccggg
     9361 tgccgggttc cggtcagtcg ggccagctcg aaaaggttta ccttcaggat ttccggcccg
     9421 gcctgcgtcc cggcggccag gagccggtgg tcgcaatcaa gcagtgtgcg gatgccgcgt
     9481 tccttcagcc gggttatcag ccggccgtag aaatcggccg ggaagccggg cggcgccgac
     9541 ccgcagacgg caaaaaaaga gccccggggg caggaattat cgcagacgaa attccagaac
     9601 gcccggagcc tctttcgatc gagccgggga ccgggttcgt tgaagcggta aatgcgcccg
     9661 gtcctgccct ctcttatgtt caatatgacc cggttttcac cctctgcccg gaaaaaccgc
     9721 cccgggatac cctcccgctt cagctggttg agtatccagc ggccggtatc accggccgcc
     9781 agtcccagcg cccgggttcg cgaacccagc gatttgagac cccgggagac gttgattccc
     9841 ttgccgccca ggtcccggcg ggcggcgcgc gaccggttga tgtcctcggg aacgatctgg
     9901 attacttcaa ggtaaaaatc ctggctgggg ttgcaggtca gggtgtatat atccatcccg
     9961 ttcaacggga acgctttttg cgcagtttct tcaaacggcg ctggcggctg ggcttctcat
    10021 agtactcccg gcttttgatt tcctggagta tcttctcccg ctcgacctcg cgcttgaacc
    10081 gcttgaagat cttttcgaaa tcgtccgatt gcctggtgcg gtgtctgatc tcgacggctg
    10141 acaaattctt cacccccttt gtccgtgacg cccggactct ttttcagacg gcgtcaacgg
    10201 cgtaaatctt ggccttgcag tagtggcagg actcctcgtt ttcgtagtag ccgtccacca
    10261 tgaagcagct ccggcagcgg atgttgctcc gaagttcgac cggcttggac gggatcttta
    10321 ccgcgaccgg cttgaccgga gcggtcttgc ggaccggggt cttgccggcc ggtttcgcgg
    10381 cggttttctt cccggcggcc ggtttcgtcg gggcggcctt ccgggtcgga ctttttaccg
    10441 ccggtttgac cggggccttc tttttggggg tcggccggac gaccgccttc tttttcggcg
    10501 gcgccttctt ggccgtggtt cgggccgtgg cctttgcgga cgccttcgcc gcgactttcc
    10561 tggcgggggc gggcttgact ttacggatgc ccttacccgg cccggacttc gggctcttct
    10621 tcgggatgct tttctccttc accttggacc tcgaatgggc cataaaacac cccccttgaa
    10681 atttggtagt gttttctaaa cggttgatta tacattatac gagccgaaaa agtcaagtcg
    10741 gaccgggttg agtcgaggcg gcctggattg cgatggttga tttgacccgg tcacagggtt
    10801 ctgctatgat aaaactttac cgtccctcgg tttgatctgt ccgccaaaaa ttgccttcat
    10861 ggatcgaatt cgcgccttga tacggctgcc gtaccttctc ctggccctgc tcctgtccag
    10921 ccggatcggc tggacggccg agccgccggc ggtaatctgg cgggccggca gctccgaggt
    10981 ccggttggcc gaggggcgag tggaggagtt tatcctggag ggcggggtgg tttttgacga
    11041 cggcgcccgc cgggtccagg ccgaccgggc ccggtacttt ccggagcggc gggaggccct
    11101 gctggaagga ggcgtggtta tcaacctgcc ggccggcgag atccgggccg agtcggcccg
    11161 ctacattctg gatcagtccg tcggcgagtc cggtccggcc gtctttgcgg cgccaccctg
    11221 gtacggccgg gttgagcgga cccggattct gagtccggac gagatccagc tcgagaacgg
    11281 ctaccttacc acctgcgacc tggttgaccc ccactaccgg atcagcctgg actcggcccg
    11341 gatcaagccg gacaagtggg tcaagatgcg ttcggccacc ttccgggtcg gccgggttcc
    11401 ggtcttctac ctgccggccg tttcgcagcg cctggactcc gggtcgcctt ttgcctttta
    11461 cgccgacccg gccatcagct cccgggacgg tttccagttg ctgaccacgg tcagttatcg
    11521 gaccgaggcc gcgcgttacg atttcgacct cgattaccgc ggtcacaagg ggcccggctt
    11581 cggtttcggt ttcgagcagg tccagcccgg ccgccggacc gagttcaaaa cctattacat
    11641 ccacgacctg gaccgccacc gggatggtta ccggggcgag ctctggcacc gttcggcctg
    11701 gccgaggtcc gagggtgagg acaccctcag gctggaggcg cacaccttct ccgaggccgg
    11761 cttccttaag gatttcttct ggcgggagag caccaccgac cgggatcgtg aaagccggct
    11821 ggcttacgcg gtcaaccgtt ccggctatta cctcggcgcc gcggtcgagg gggaactgga
    11881 cgatttttac aaccggaccg agtacctgcc cgtcctgcgt ctctaccggc cggcgcgttc
    11941 cctgggaccg ggatactggc agggcgacct ggaggccgcg gcgctggccc gggacttcgg
    12001 cggccgccgg cgggagaccg gccgggtgca cctggtggag acgctttccg gtttccggcc
    12061 gctgctgggc ggaacctggc gtccctttgc cagcgccggc gccaccctct actccgggac
    12121 gaccgaagga tcggagaacc gggtgcggag cctcttccag tccggcttca acctggactg
    12181 gaagttcggc cgccgttacg ggccggagga ggcggaagaa ggctggaccc attacctgga
    12241 accccggatt tccgtcctga gccgggatat cggcgaacgt cccggccgtt tctaccagta
    12301 cgacctgatt gacagcctgg attccgacca ggtcgtttcc ctccagttga ccaaccggct
    12361 gctggcgggg ccgtccgggg aagggcggga ggcgttgcga ttcgacctga aaggagacta
    12421 ctccttgaag cggggccgtc cgggcgatct ctactcgctt atcaattact ctcccgaccc
    12481 ggccttttcc ttttacagcg agggggactg tgacctggac cggctggcct ggcggagtct
    12541 ctccagttcg atggtctggc atcgtccgcc ctttgatttt ggcctttcgc acagctacca
    12601 gcgcaacgaa agcgagctgc tcacgccttt tgtttccctg ccgctcggcc gcaggtggcg
    12661 ccttgagacc cgggtggcct tcaacctgaa ggacgacggc ctggagagcc gggaggtcag
    12721 cctctggaag gacatgcatt gctgggaagg ccggttcggg gtttatcacg accgcaagga
    12781 aaccgaggtg tacctcttgt tctacccgaa gattttcccg gggcgcagcc tcaagataaa
    12841 gtccctgatt gactgagaac cgggatcggt ttcttgagtt cgaggccgct tcttggttat
    12901 aatataaccc tttaaaaagg cggtgtagct caggggaaga gcaggcggct catacccgct
    12961 atgtccgggg ttcaaatccc tgcaccgcca ctctttcgat tgacgtgaag atgccgctga
    13021 agatcctgat cgccccggat tcgttcaagg gcagccttac cgccgccgcg gccgccgcgg
    13081 ccatccggac cggttttttt cgcgtctggc cccgggccga ataccgtctt ttgccgctgg
    13141 ccgatggtgg cgaaggaact ctggcggcct tgctggcggc ccggggcggc cgtcgttttc
    13201 ggttggaggt ccgcggtccg cgcggccgcc cggtccgggc ggtttgcggc cagcttccgg
    13261 atgcttccgg ggtggtcgaa atcgcctcgg ccggcggcct cccgctgctg gaagcctccg
    13321 aacgcaatcc ccgcctggcc tcaagctacg gaaacggcca ggttatccgc tttctgctgg
    13381 gccgcccggt ttccgggctg ctgatcggtt tgggcggcag cgccacggtt gacggcggcc
    13441 tgggaatgct gacggccctg ggcgcccgtt tcctggatgt tcggggacgg gtgctctccg
    13501 gcatcggcgg cgatctggaa agagtgcgcg ccatcgatct ttcaaacctg gactgtcgtt
    13561 tgcagggatt gccggtaacg gctttgtgcg acgttgacaa tccgttgctg ggcgttcggg
    13621 gggcggcccg cgttttcggc ccccagaagg gtgccggccc ggcgatggtg gagcgcctcg
    13681 aggccgggct ggccaacttc gcggaacggg tcagatccga actcggcttt gacggcgccc
    13741 ggaggccggg tgccggcgcc gcgggcggcc ttggttttgg actggccgcc tgcctgggtg
    13801 cccggctggt taacggcgcc gacttcataa tgaagtcgct cggactggaa cggttcctgg
    13861 cctggtccga cctggcggtc tccggcgaag gccgggtgga cgggcaggtg aagttcggca
    13921 aggcgctcca ccgcgtgggt ggcctggccc ggcaagtcgg ccggccgctg ctggtcttct
    13981 gcggagaacg cgacctggcg gatcgtgaaa ggcgccggct cggatttacg gcggtctttg
    14041 ccatcgagcc cggaccggtg accggggccg aggcgatggc ccgggcccga atttacctgg
    14101 cggatacggc ggccgagacg gcccgtttga tggccggcac tcccggccgg ttcgcccggg
    14161 actgacggag agattgttta acgaaacatg tcctacgaag cgctggccct gaaataccgg
    14221 ccccggacct tcaaggagat ccgcggccag gaggagataa ccgccaccct gcgccgttcc
    14281 atcggcgaag ggcgcatcta cccggcctac ctgctttcgg gtccgcgcgg cgtcggcaaa
    14341 acctccaccg cccgcattct gacccggagc ctgaactgtg tcaaggggcc ggccgaggag
    14401 ccctgcaaca agtgcgagac ctgcctggag atactcaacg gaaacagcct ggattttttc
    14461 gagatcgacg gggccagcaa ccggggtatc gaggaggtcc ggaccatcaa cgagaacacc
    14521 cgtttcctgc cggtcaagag ccgttacaag gtttacctga ttgatgaagt ccacatgctg
    14581 accgaccagg ccttcaacgc cctgctcaag acgctcgagg aacccccgga acacgtccgc
    14641 ttcatctttg ccaccacgca ccccgataag ctgcccggca ccgtgatttc gcgctgccag
    14701 cgttacgatt tccacctcat acccggcccg gtgctggtcc agcacctgca ggagatcgcc
    14761 ggcaaggagt caatcgacat cgagccggcc gccctggtgc gcatcagcca ggcggccgcc
    14821 ggttccttcc gggacgggat cgcggtgctt gaccagctgg tctccttccg gtacggtcag
    14881 cagataaccg ccgttgatgt cgagaagctc ttgagcctgg tcccggaaga ggagtacctg
    14941 gatgttttcc aggcgctcgt aaccggagac ggccgccgct gcctcgagaa ggtgggcgcg
    15001 ctcagccagg ccggttacga cctttacaag ttcacttccg gcctgctgag tttcatccgt
    15061 tacctgctgc tcctcaaggc cggcgccggc gatctgatcc aggatgtggt tactcccgag
    15121 accgagcggg cactggtcga atgggcgggt cggctggaca tcgagacgct gctggcgctg
    15181 cttgaagggc tggccgacga acgcgagaag ttgagatttg aagacctggt gcgggtgcgc
    15241 ctggaagttt ccctgctgaa gctggccggc cgggccggcg gccggacacc ggtgattgat
    15301 tccggaggcg gacgttccga ttcggcccgg ccggcggtcc ggctgaagcc ggtttccccg
    15361 gccccggagc ccgttaagcc gccggcgtcg gagccgccga agcttccccc gaccgccccg
    15421 tccgttgcgc cggcgtcgga actgtccgag gcgtcaccgg cggcaataga cgccccgccg
    15481 ccgctgggaa ccggggccgc cgcttcggac gccgccctgg ccggggcgga ggcgtcggcc
    15541 gttctcgacc cggccgcctg ctggccgggt ttcctgaatg aattgctgcg ccgggagccc
    15601 ttcttccatg cgccgcttca gcagggcggc ctccaggaga tccgtaccgc ggagaagccg
    15661 ccggtcctgg tggtcggcat caacaacctt ttcgaacagg atcttttgaa gaaaaacagc
    15721 cggcgcctgt cggagatctg gcaggaatgc tacggaatgt cagttcgttt tgaagcggtc
    15781 cgcttgccgg aaaaggggat cggtcggatt cccgggccgc cgccgggcct gaaggagacc
    15841 gcctcgaaaa aggtccggcc gccggcgccg gcggcgccgc cggcgggcga aacgacgccg
    15901 gcacgggaga ttcccgcgac cggaaccggc agcgccagcc tttcgcccga cgaagaggcg
    15961 atggtccgcc ggatccaggg aatcatgggc ggtgaagtta aggagatttt caaggaataa
    16021 accggacaaa ggagaaaaac aatggcgaat ttcatgcaaa acctgaaaga gttgaaccag
    16081 ttgcgccaga cggccaaggt tatcgagaag aaacttcggg agcagaaacg cgaattttcc
    16141 agtcgggacg ggagaatcaa gggggtcatc aacggcaagc ttgagatcct ttcgctggag
    16201 gtggctccgg aactgctgac cgccgagggc aaggccgccc tggagcggct tctgatgtcc
    16261 accatcaaca acgcggtttc cgatatgcag agccaggttt cgaaggtggt cagcagccag
    16321 atgggcatca atctgcccgg cctgggattt taaagtcggc cggggcgtat cgggggtgaa
    16381 tcggtgttcc tattcttcgt ttacaacttc tcggcctttc tggccctgca cctgccggag
    16441 ccggccgccc ggacactggc ccgggtgacc tcggcgctct tttacctggt caccccgggc
    16501 atgaagcgga atgcccggcg caatttccgg gtaatgctct ccagcggggg gcgggaaccg
    16561 acggccggag agctggaacg cctggtctgg cgcgcctacc tgaatttcgg cctctatctg
    16621 tatgaatttt tcctgatccc ccgcattgac ggggagtttc tgaaccggcg cctggaggtg
    16681 cggggactgg agcacctcga ttcggccatc ggcggcggcc gcggcgtcat cagcatcacc
    16741 ggccacctcg gtaactggga actggccggg atcttgacca gcatgatggg ttacccggtt
    16801 accgcggttg cgctcgacca ccgcagtccg agcatgaaca agttctttct gcaccgccgc
    16861 cgcatgaaag gcgtgaagat cgttgcggtc ggcggccgga ccctggccct gatccgggcc
    16921 ctgaaaaagt ccggcatcgt tgcgctgctg gccgaccggg tcttttcccg gtcccggctc
    16981 cggatggatt ttttcgggcg ttccacctat ctgccggccg ggccggccga actcgcggtg
    17041 ctgaccggca gcccgatcgt gcccggcttc ctggtccgcc ggggccgccg ctacctgctt
    17101 tccttcgaac cgcccatcca cccggacctg gcctccgggc gcagctcggc ggtgcgcacc
    17161 ctggcccggc atgtcattga cgtgctggag cgctacatcc ggacttatcc ggaacagtgg
    17221 ctggtcttcg aatacctatg gaacaaagaa accgcgtttt gatgtcgggc aatgaggtgg
    17281 ccggagaggc ggcggtcgcc gccggctgcc ggttttacgc gggctatccc atcacgcccc
    17341 agaaccagtt gaccgagtat atgagtcgcc gggttccgga agtgggcggg gttttcatcc
    17401 aggccgaaag cgaactggcc gccatcaaca tggtcttcgg cgccgccgcc gccggcgccc
    17461 gggcgatgac ctcctcctcc agcccgggca tcagcctcaa gcaggagggt atttcctacc
    17521 tggccggctg cgagctgccg gcggtgatcg tcaacatgaa ccgggccggg cccgggctgg
    17581 gcgatgttac gcccagccag gccgattact ttcagtcgac gcggggcggc ggccacggcg
    17641 attaccggac catcaccctc ggcccgcaga acctccagga actttacgat ctcacctacc
    17701 tggcctttga actggccgac cgttatcgca acccggccct catcctgggc gacggcatcc
    17761 ttgcccaggt catggaaccg gtccggttga agcccctgcg ccgggtccgg cccgccccgg
    17821 cctgggccct gaccggctgc ggagacggcc cgccgcggaa gatcctgtcg ctttttctga
    17881 agcccggggt tgccgaggcg cacaactggc gcctggccgc caagtaccgg agaatcgagg
    17941 ttcgcgaaca gcgctgggac gagttccagg ttgaagacgc gaaactggtg gtagcggcct
    18001 tcgggagttc cgcccgcatt gcccgggcct cggtccaggc cgcccgggag gaaggcctgg
    18061 cggtcggcct cttccggccg gtttcgctct ggccgtttcc ctaccagccc ctgcggcggc
    18121 tggccgaggg ccggcgccgt ttcctggtcg tcgaaatgaa cctgggccag atggtcgaag
    18181 acgtgaggct ggcggtcgag aaccgggggc cggtctcctt ttacggccgg ccgggcggcg
    18241 gcgtgcccac tccggaggag attctgaagg tgatccgaaa aacctaccgg ggggcggagc
    18301 gatgagccag acctaccttt tcaagcggac cgagggattg caggacgcgc ccaccagttt
    18361 ttgccccggc tgcggccacg gcatcctgct gcgcctggtg gccgaactcg tttgggaact
    18421 gggcctgcgc ggccgaaccg tcggcgtggc tccggtgggc tgcgcggtga ccgcctacga
    18481 ctactggaat ttcgatgtga ccgaggccgc ccacggccgg gcgccggcgg ttgccaccgg
    18541 catcaagcgg gttcatccgg accggctggt ctttacctac ccggg
//
LOCUS       DAOQYN010000011        18056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_3500_length_18056_cov_4.633520, whole genome shotgun sequence.
ACCESSION   DAOQYN010000011 DAOQYN010000000
VERSION     DAOQYN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18056
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3500_length_18056_cov_4.633520"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /gene="dctP"
                     /locus_tag="PKN80_01005"
     CDS             complement(<1..661)
                     /gene="dctP"
                     /locus_tag="PKN80_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNQ34626.1"
                     /translation="MRIWKIWLALVILAGCLAETLAAQEKPKPVNLTYSIFFPAPHRN
                     TVLATEWAKEIEKRTGGRVKITIYPGGTLTPSDKCYDGVEKGISDLGMSAFAYTRGRF
                     PLTEVIDLPLGYKSGLQATRLINAYYRKFTPKELDKVKVMYLHAHGPGLLHTKTPVRR
                     MEDLKGMKIRCTGLAAKIVEELGGTPVAMPMGDTYDALSRGTVNGSMGPQESLQGWKW
                     GE"
     gene            complement(845..1876)
                     /locus_tag="PKN80_01010"
     CDS             complement(845..1876)
                     /locus_tag="PKN80_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNQ34627.1"
                     /translation="MAGTFRFSFGPWNIHTGADSFGPPVRKPLAFAKKLEFYRELGFG
                     AVQFHDDDAVPGLDELSTLQAERKAKETARLLKSSGLAAEFVAPRLWESPRTVDGAFT
                     SNNPADRTYARERCRKAVEIANRLETDLIVLWLAREGTYIREAKDSKWAVERLVEGVN
                     RILACDPKIRIAIEPKPNEPMDHAFIPTTGHAVALAGLTDDPARVGVNIESAHAILAG
                     LDPAEEMGFALASRKLFTVHLNDQNGLKFDQDKSFGSVDLRRALNQVWILDRSGYGKN
                     GEWVGLDVKAMRTQPAPAVKHLRNSREIFLRLLKVSRGLDESKIASLVRERDYEELDL
                     YIMERLLGK"
     gene            2166..3737
                     /locus_tag="PKN80_01015"
     CDS             2166..3737
                     /locus_tag="PKN80_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ34628.1"
                     /translation="MNTLTGRRRRMRIIIPAYPAFNIYTHIARRTTALGPICVATVVH
                     ELPGWDVEVIDENNLAGYGPRGENGGADHDFLQRLRPADAVGFYGGLTSTIPRLLKLA
                     GFYRRQGVLTVAGGQHFCAETAAEGLAGGLDYIVLGEGETTIRELLSVLDAGGCRADV
                     KGLAYLENGKIVFTPPREPVTDFDRLPLPDFSLVRNARISLYPVERIRGCGMDCEFCA
                     VKGKFRSAAPERLLAQISYLLETRAARHFFIVDDLFGQQREETLRFCGLLRDYQREIG
                     RRLDLTVQIRLDKARDSELLSAMRQAGINTVCIGFESPIPEELTAMRKHVQPGEMVEL
                     ARIFHRFGFLVHGMFIFGYPLESGADFQLPLRKRIKRYRDFVRRARIDTLQVLLPVPL
                     PDTELRRRLQAQNRVYPTVAVGWEYYDGNFPLFEPDPPLRAEDLQAALRSIMGKFYQL
                     RYLPVIGWNALSFSGLLFFLHDIRFGWRQWYRSWRNTWVRFGGWIIFTRWIRAYEKGD
                     FSRRLLQARAELKKE"
     gene            complement(3758..4594)
                     /locus_tag="PKN80_01020"
     CDS             complement(3758..4594)
                     /locus_tag="PKN80_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNQ34629.1"
                     /translation="MKFKAALVQMRVEGGKKRENLQRARKLIAAAAGAGARLALLPET
                     MDLGWTHPASQSEAEPIPDGLPFQTLAGAAAESRIFVCAGLTESDGKRIFNSAVLIDP
                     RGRLLARHRKVNELAIGQAYYAAGDRLTVIQTELGSVGLMICADAFARDRVLSRSLGY
                     LGAEIILSPCAWAVPGEHDNAAEPYGDTWRKSYRPVAAEFSTWIVGTSSVGPINGGPW
                     AGRKCIGCSLAVGPDGREVLQGPYGVDAETILYLDIEPVARPARGDSWMEYWKNRPES
                     IG"
     gene            complement(4662..5900)
                     /locus_tag="PKN80_01025"
     CDS             complement(4662..5900)
                     /locus_tag="PKN80_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ34630.1"
                     /translation="MNSLDFMLFCLQLILMLSFALAFGQLAKKIRQPAVFGELLGGII
                     LGPTVFGAIAPGFHATIFPAAGQVAFVRETLIKLGMLFFLFTAGLEVNPAGLKKSGRP
                     ALFTSLFGIVVPFVFGMAMVKALDWLWLPRIGIGINLFAFFMGTALSISALPIIARIL
                     MDLKLTRDPIGDVIMTAAVINDLFGWTIFALIIGAIRPAGGLEIQKLPLMLAELAVFV
                     WLVFALGRGLGRNPLARFMNQSGPSGSRLMLLTILMLTAALSTEMIGIHAIFGAFLLG
                     VALDFDRGENNHAHEIIGRFAIGFFAPLYFVSIGLRANFIADFNLPLFLLILLTASAG
                     KILGSGAGARAGGMLPREALAVGFGMNARGVMEILLASVALEYAIINRQVYVALVAMA
                     LISSMASGPLMKMVLKPSGR"
     gene            6181..6522
                     /locus_tag="PKN80_01030"
     CDS             6181..6522
                     /locus_tag="PKN80_01030"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ34631.1"
                     /translation="MKELEDFLRAVGDDKRLRILKMLERRRLCVCEITAVLGLRQPTV
                     SRHLRRLEKVGLIEQEQNGYYRECRLVSGHPFHDLWMWLSERLEKSAEIRADARKLAR
                     VDRRRLACRGG"
     gene            6581..7765
                     /locus_tag="PKN80_01035"
     CDS             6581..7765
                     /locus_tag="PKN80_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNQ34632.1"
                     /translation="MKTRLSEPAKLLLMLSLFLFSFYLPVDWLPFRNPVYEAFALLKW
                     YAREHVLLCLVPAFFIAGAISVFVSQAAVLKYFGGRANKWLAYGVASVSGSVLAVCSC
                     TVLPLFSGIYRRGAGLGPATAFLYSGPAINVLAIIMTARILGWRLGLARAVGAVLFSV
                     VIGLLMHLIFLKEEQHRHQAGDLFTGDETERRSLRQETLYFAALVGILVFANWSKPPA
                     AGLGPWAVVFQLKWFITGLLGLALLAMLKAWFAKEELTGWIQAAWTFAKQIFPLLLAG
                     VLVSGLLLGRPGHEGLIPSRFVAALVGGNSFFANLFASIVAAFMYFATLTEVPILQGL
                     IGSGMGQGPALALLLAGPALSLPSMLVLRSVLGTKKTVVYVLLVIGLSTLAGMLFGAL
                     AG"
     gene            7782..8567
                     /gene="arsM"
                     /locus_tag="PKN80_01040"
     CDS             7782..8567
                     /gene="arsM"
                     /locus_tag="PKN80_01040"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNQ34633.1"
                     /translation="MKKEAGEIRRLVREGYGRAVKRKAAGAASACGCCNAGAAPERSR
                     RAGYSDKEMKAVPGGANLGFGCGNPLAGSEIKRGEVVLDLGSGAGFDVFLAARLVGPA
                     GRVIGVDMTPAMVRKAERNARQGRFTNVEFRRGEIEKLPVADASVDVVISNCVINLSP
                     EKLRVFREAFRVLKPGGRLIVSDLVLKKRLPDFLRNSLEAYLGCLAGAVLENAYLKLI
                     RKAGFEDVEITGRSGYPVDDCFPEARGAGDLISSLTVRAVKKN"
     gene            8584..8814
                     /locus_tag="PKN80_01045"
     CDS             8584..8814
                     /locus_tag="PKN80_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ34634.1"
                     /translation="MKVEILGLGCPKCKLLTANAESAARELGLELEIVKVTDIRKISE
                     YGVMMTPALVVDGVVRSAGKVLPVEAIKKLLR"
     gene            8853..9299
                     /locus_tag="PKN80_01050"
     CDS             8853..9299
                     /locus_tag="PKN80_01050"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNQ34635.1"
                     /translation="MEERKRRVLFICVHNSARSQMAEAFLKHLAGDRFEVESAGLEPG
                     ILNPDAVEVMKEVGLDISRNRTKSVFELLKAGRSYDYVITVCDESRAGECPIFPGPAA
                     SRCHWTFDDPSAFEGTGAEKLEKTRIVREAIRERLERWLGEIGGRG"
     gene            9403..9972
                     /locus_tag="PKN80_01055"
     CDS             9403..9972
                     /locus_tag="PKN80_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ34636.1"
                     /translation="MADSRKPSLTRFLAWQILAILLIVGIAAALILNKRRPSVSPAAV
                     APAPAASSASGTPGEIRREKSTADQKPAPGPAAPAAGSEPSAAAQEVGLPRLLELGAD
                     SCVPCKMMVPVLDGLRKDYAGRLKVEFIDVYEDSRAGVSHRVRVIPTQIFFDASGNEL
                     SRHEGFMSREDILAKWGKLGYDFLPAAGE"
     gene            9969..10670
                     /locus_tag="PKN80_01060"
     CDS             9969..10670
                     /locus_tag="PKN80_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNQ34637.1"
                     /translation="MMSGILAFLGRAIEGAPLVALLASAAWGVLSLLLSPCHLAAIPL
                     IVGFIDEQGRLSRRRAFLISVLFAAGILLSVTMVGVLTAVAGRMLGDIGRYGNYFVAA
                     VFFMAGLHLAGLFPFSLSAPQGLPTGKKGAAAAFMLGLVFGIALGPCTFAYLAPILTV
                     AFKTAAADFYYGFLLLVAYGIGHSAVIVLAGTFIGTVQRYLDWNEKSKGARLLKKTCG
                     LLVILGGLYLVYTAR"
     gene            complement(10699..12234)
                     /locus_tag="PKN80_01065"
     CDS             complement(10699..12234)
                     /locus_tag="PKN80_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34638.1"
                     /translation="MKHLIPEAKNALLCLGMICLAITWPAATRAQEAKVNESVIELGT
                     RREIFVDHHLIERLNGARLVLHQPRDEGMIFKFDQPWEGNDGIYAAVIKDGSKYRFYY
                     RVQTQPNKGDTDPNQAICYAESPDGINWTKPELGLYEAAGSKKNNIVFMTQLPVCNNF
                     TPFLDTRPGCPAGERFKALGGYRSLGLFAYVSEDGIAWRKIRENKDPLQEAVIKKGQG
                     RFDSQNSAFWSEAEGKYVCYLRESLGGSYRWIARTTSPDFLEWTPAELMEARCNDKAA
                     PPEHLYTNQTHPYFRAPHIYIAIAARFMQGRSAVTPEQLEPIYRGADKPAPAWISKDC
                     SEAVFMTSRGGNLYDRTFMEAFIRPPIGFENWTSRANYPALNVVQTGPAEMSFYVHRS
                     YTQPGAHMRRYSLRLAGFASIQAPYDGGEVLTRVLTFSGRQLRLNYSTGAAGGIRVEI
                     QTADGRPVPGFSLKESRELLGNEIEGTAAWTGSGELAGLAGTPIRLRFVMKDADIYAL
                     RFE"
     gene            complement(12259..13335)
                     /locus_tag="PKN80_01070"
     CDS             complement(12259..13335)
                     /locus_tag="PKN80_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNQ34639.1"
                     /translation="MKRWSVEKAAAWYREQPWLVGCNFLPSNAVNQLEMWQAETYDPV
                     TIDRELSWAEGLGFNTLRVYLHDLAWADDRTGCAERIDDFLERARRRGLRVIPVLFDD
                     CHRPDPASGPQPQPLAGVHNSEWKQSPGQEIVRRFNEGTAPEGEKQRLSEYVRGVISR
                     FAGDERVLMWDIYNEPGQGGNGDRSNELLELAWDWARQAGAVQPLTACLNGSRGEKNL
                     VLNRSRSDVITFHGYDGGTLEKIIVEQKQENGGRPVICTEYMARELGTTFQHSLPIFR
                     KHRVGCLNWGLVAGKSQTHFNWQTARKIEILRKTGRRLRPGEPIPEPPLWFHDIFRTD
                     GTPFDPEEINYIRSVTKPELKTGR"
     gene            13847..16150
                     /locus_tag="PKN80_01075"
     CDS             13847..16150
                     /locus_tag="PKN80_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34640.1"
                     /translation="MGFLLLAVQARAQVTPGTYQRIDGNPLRIDAYEDGTLGVYRWNG
                     TDYINQYYSGNCWGSVLLLHSPRTTDAFNSWYFIYWGYTNAKPQFTPVSNTKPDPWTT
                     VTVFRAGDSGVRLTQTIKYTDGNAYYKKSWRIENLGSTEYNDCRFFHGGDSTFGGIDA
                     SRGHWDANLGMVYLTNPDQAIDGIMGFYGDLTTPATSYAEGEYDDEVCERILDTGQLG
                     NSVNPGYIDAGYALQWNRGTLSPGDVWTIISYEKWTEAGFVQVFAPAEREVEAGDVIQ
                     HQFVVANYQATTDTFILQPDSSLDWPWAFVNPAQAQVTLAAGASTTVSFQVTIPAGAA
                     PGTRDVLTLTATSQTTPAVTNQDSVATVIPGVLAGIVSKLQGGGGPCFIATAAYGSYQ
                     EPHVQVLRRFRDRFLLASPPGRAFVRFYYRHSPPLAAVIARNETLRAATRTALLPVYG
                     VAWLCLNGRSVLAGLLGSVLLAGLFLLAGRRRFRLAAGVTAVILLLGAVPASALDINN
                     FSPAIGGDSFTLLPASKVLPARNFSADLYLVYVEKPLRGNVGGKWTVLSEDQTLATLG
                     LGLGVTDSFQLGLRLPYLFSQGSQLTGAAAPGDGDIGDLRLEGKYRVRGGPDQVGVAL
                     LPYVTLETGEEDSYFGKGSNAFGLTAVLDRNFGDRVCLAFKVGYQYQEKENLPDFDLE
                     DTLLFGAGIGWKLPNDRTRASVELQGLTNASALFNSEDATPVELLGSIHHPVNENLLL
                     DLGLGVGLTDGYGAPEYRLFLGMRTGF"
     gene            16337..16657
                     /locus_tag="PKN80_01080"
     CDS             16337..16657
                     /locus_tag="PKN80_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34641.1"
                     /translation="MNVFEAIPYADEPFGRRKVVDQKHLLVMQIALKPGQAVPPHEAN
                     SNVHLLVLDGSVVVSLSGQDTLARRGDILPVAFRTPMRIRNESGANATFLVIKTPNPS
                     EMGG"
     gene            16672..17028
                     /locus_tag="PKN80_01085"
     CDS             16672..17028
                     /locus_tag="PKN80_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNQ34642.1"
                     /translation="MKRIQVKRVYEPAGPADGVRVLVDRLWPRGLSKAKAAVNEWLKE
                     AAPSQVLRQWFSHDPRKWPEFRKKYFEELDSRPEVVFKLRQLLRKGPATLLYGARDEN
                     FNNAVALAEYLKKKAG"
     gene            complement(17132..>18056)
                     /locus_tag="PKN80_01090"
     CDS             complement(17132..>18056)
                     /locus_tag="PKN80_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34643.1"
                     /translation="FMTSSEADLDKPHTLSLPAPQGTRFYDLFANELKPKQNGERLEV
                     TVPVLPVYVTLPAAGLEAFLARLETVKLAREAQPSFKRFETGPIVVHVDQNDKGILKV
                     FLKKGNRLEPVINGIQTDVPFGDRMQISAEGNGIVTSVRIDDPYNPPGRKYLALMADQ
                     DGLTFTVRADSWRGDSPKEGFVRLMLGEYILDRNYAYYYRPAAGPVTGLGGQIAHDFG
                     KLTPTGPAAVPAGFSLKEGTAILEIADAYRVSLFAPAPDPNIKLLTPPGFQFFVRNGE
                     AGLESRFHLTTPPEGVKRIIFTTGLELKNLR"
BASE COUNT         3078 a         5521 c         5706 g         3751 t
ORIGIN      
        1 cctcgcccca tttccagccc tggagtgatt cctgggggcc cattgaaccg tttaccgtgc
       61 cccggctcag ggcgtcgtag gtgtcgccca tcggcatcgc caccggggtg ccgcccagtt
      121 cttcgacgat cttggcagcc aggccggtgc agcggatctt catgcccttg aggtcttcca
      181 tccgccggac cggggtcttg gtatgcagca atccgggtcc gtgggcgtgc agatacatca
      241 ccttgacctt gtccagttcc ttgggggtga acttgcggta gtaggcgttg atgagccggg
      301 tggcctgcag tccgctcttg tagccgagcg gcaggtcgat gacctcggtg agcggaaagc
      361 gtccccgggt gtaggcgaag gccgacatgc ccaggtcgga aatgcccttc tcgacgccgt
      421 cgtaacattt gtcggaagga gtcagggtgc cgccgggata gatggtgatc ttgactcggc
      481 cgccggtccg tttttcgatc tccttggccc attcggtggc cagaacggtg ttccggtgcg
      541 gggccgggaa gaagatcgag taggtcaggt tgacgggctt gggcttttcc tgggccgcca
      601 gcgtttcggc taggcagccg gccaggataa ccagggccag ccaaatcttc caaatcctca
      661 ttccgcctcc ttgttgatcc ggccggttcc ggccgggctt gagaaaagag ggccttcccg
      721 ggtttgcccg ggaacaggac acagggatat tgtagaagaa aacccgattg atttcaaggc
      781 gttttaccgg cggcccggtc cggatgccgg ccgcgcttcg gattcggcgg gcggtgcttc
      841 cggattattt tccgaggagg cgctccatga tgtaaaggtc taattcctcg taatcccgct
      901 ccctgaccag gctggcgatt ttcgattcgt ccaggccgcg gcttactttc agcagccgca
      961 ggaagatctc ccggctgttc cgcaggtgct tgaccgccgg ggccggctgg gtccgcatcg
     1021 ccttgacgtc caggcccacc cattccccgt ttttcccgta cccgctgcgg tccaggatcc
     1081 acacctggtt caaggcccgg cgcaggtcca ctgagccgaa ggatttgtcc tggtcgaact
     1141 tgagaccgtt ctggtcgttg aggtggaccg tgaagagttt tcggctggcc agcgcgaagc
     1201 ccatctcctc ggccgggtcc aggccggcca ggatggcgtg ggcgctttcg atgttgaccc
     1261 cgacccgggc cgggtcgtcg gtcaggccgg ccagcgcgac cgcgtggccg gtggtcggaa
     1321 tgaaggcgtg atccatcggc tcgttgggct tgggttcgat cgcgatgcgg attttcgggt
     1381 cgcaggccag gatccggttg acgccctcca ccaggcgctc caccgcccat ttgctgtcct
     1441 tggcttcccg gatataggtt ccttcgcggg ccagccagag gacgatcagg tcggtttcga
     1501 gccggttggc gatttcgacc gccttccggc aacgctcccg ggcataggtc cggtcggccg
     1561 ggttgttgga agtgaaggcc ccgtcaacgg ttcgggggga ttcccagagc cggggagcca
     1621 cgaactcggc cgccaggccg gacgatttca gaagccgggc ggtctccttc gccttccgtt
     1681 ccgcctggag tgttgaaagc tcgtccaggc cgggcaccgc gtcgtcatcg tgaaactgga
     1741 ccgcgccgaa gcccagctcc cggtaaaact ccagtttttt ggcaaaggcg agcggtttgc
     1801 gcaccggcgg tccgaacgaa tccgcccccg tgtggatgtt ccagggtccg aaagagaatc
     1861 gaaaagtccc ggccatttta ctcctcccgc ttgcggcgtt cattttttta acttggatgt
     1921 ccggttaatc acgggcgttt ctgtcgcgga acggaacggc gccatcccgg cttttctgat
     1981 tttatctttt atgttcctgg ctggtcaacc cggcttcccg ctttttcccg cccttcccgc
     2041 tgaaagatcc ggaggcccgt ccggtgttga cgccgccggt attagaaggt attattctga
     2101 tgggaatccc ggtggcggaa aaaccggggt catccggaac ctttgccccg taaggttgga
     2161 aaatcatgaa cactttaacc ggccgtcgcc gccggatgcg catcattatt cccgcctacc
     2221 ccgctttcaa tatctacacc cacatcgccc gccgcaccac cgccctggga ccgatctgcg
     2281 tggccaccgt cgttcatgaa ctgcccggct gggacgtcga ggtgatcgac gagaacaacc
     2341 tggccggtta cggtccccgg ggtgaaaatg gcggcgccga ccacgacttt ctgcagcggt
     2401 tgcggccggc cgacgcggtc ggcttttacg gcggcctgac cagcaccatc ccccgcttgc
     2461 tgaaactggc cggtttctat cgccggcaag gcgttctgac cgtggccggc ggccagcatt
     2521 tctgcgctga gacggcggct gagggtttgg cgggcggcct ggattacatc gtgctcggcg
     2581 agggggaaac gaccatccgg gaactgcttt cggtcctcga cgccggcggc tgccgggccg
     2641 atgtcaaagg gttggcctat ctggaaaacg ggaagattgt ttttactccg ccgcgggagc
     2701 cggtgaccga tttcgaccgg ctgcccctgc cggacttctc cctggttcgc aatgcccgga
     2761 tcagccttta cccggtggaa cggatccggg gctgcggaat ggattgcgaa ttttgtgccg
     2821 tcaagggaaa attccgctcg gccgcgccgg aacgcctgct ggcccagata agctacctgc
     2881 tcgaaacccg ggccgcgcgc catttcttca tagtcgacga tctcttcggt cagcagcggg
     2941 aggagacgct ccgcttctgc gggctcttgc gagattatca aagggaaata ggccggcgac
     3001 tcgacctgac cgtccagatc cggctggaca aggcccggga ttcggaactc ctgtcggcca
     3061 tgcgtcaggc cggcatcaac accgtctgta tcggtttcga gtcgccgatc cccgaggaat
     3121 tgaccgccat gcgcaaacac gttcaacccg gggagatggt ggaactggcc aggatcttcc
     3181 accgcttcgg tttcctggtg cacggcatgt tcatcttcgg ctatcccctc gaatccgggg
     3241 ctgatttcca gctgcccttg cggaaacgga tcaagcgcta ccgggatttt gtccgccggg
     3301 cccggattga cacccttcag gtgctgctgc cagtgcccct gcctgatacc gagctgcgcc
     3361 gtcgcctgca ggcgcagaac cgggtgtacc ccaccgtcgc ggtcggctgg gagtactacg
     3421 acggcaattt cccgctcttc gagcccgacc cgcccttgcg ggccgaggac ttgcaggcgg
     3481 ccctgcgcag catcatgggc aagttctacc agcttcgcta cctgccggtg atcggctgga
     3541 acgccctttc tttctcggga ctgctcttct tcctgcacga catccgtttc ggctggcggc
     3601 agtggtaccg ttcctggcgg aacacctggg tgcgtttcgg cggctggata atctttacgc
     3661 gctggatccg ggcctatgag aaaggcgatt tttcccggcg gctgcttcag gcccgggcgg
     3721 agttgaagaa ggagtaaccg attcgggtgg aagattttta tccgattgat tccggtctgt
     3781 ttttccagta ttccatccag ctgtcgcccc gcgccggccg cgccaccggt tcgatatcga
     3841 ggtaaaggat tgtttccgca tcgaccccgt aaggaccctg gaggacctcc cggccgtccg
     3901 gaccgaccgc cagtgagcag ccgatgcact tccggccggc ccaggggccg ccgttgatcg
     3961 gccccacgct gctggtcccg acgatccagg ttgagaactc cgcggccacc ggtcggtagg
     4021 acttccgcca ggtatccccg taaggttcgg ccgcgttgtc atgctccccg ggtacggccc
     4081 aggcgcaggg ggaaaggatg atctccgccc ccaggtatcc cagcgagcgg ctgagcaccc
     4141 ggtcccgggc gaaggcgtcg gcgcagatca tcaggccgac gcttcccagt tcggtctgga
     4201 ttaccgtcag ccgatctccg gccgcgtaat aggcctggcc gatggcgagc tcgtttacct
     4261 tccgatgcct ggcgagcagc cttccccgcg ggtcgatcag caccgcgctg ttgaagatcc
     4321 gtttcccgtc cgactcggtc agtccggcgc agacaaagat tctgctttcg gccgcggccc
     4381 cggccagggt ctgaaagggc aggccgtccg gtatcggttc ggcctcgctc tggctggcgg
     4441 ggtgcgtcca gccgaggtcc atcgtttcgg gcaggagcgc cagccgggcg ccggccccgg
     4501 cggcggccgc gatcagcttc cgggcccgtt ggagattttc ccgcttcttt ccgccctcga
     4561 cccgcatctg gaccagggcc gccttgaatt tcattttgtt tgttctccgt ttccgttttt
     4621 cgtcttcttc ccgaccggga ggtttggctt gtttaagggt atcatcggcc ggaaggcttc
     4681 aggaccatct tcatcagcgg gccgctggcc attgaggaaa tcagcgccat ggccaccagg
     4741 gcgacgtaca cctggcggtt tatgatggcg tattcaaggg ccactgaggc cagcagtatt
     4801 tccatgactc cgcgggcgtt cattccgaaa ccgacggcaa gtgcctcgcg ggggagcatg
     4861 ccgccggccc gggcgccggc cccggagccc agtatctttc ccgcgctggc cgtcaggaga
     4921 ataaggagaa agagaggaag gttgaaatcg gcgatgaaat tagcccggag tccgatcgag
     4981 acgaaataga gcggagcgaa gaaaccgatt gcaaaccggc ctatgatttc atgcgcgtgg
     5041 ttattctctc cgcggtcgaa atcgagggcc acgcccagga gaaaggcgcc gaagatcgcg
     5101 tgtattccga tcatttcggt cgacagggcc gccgtcagca tcaggatggt caggagcatg
     5161 agacggcttc cggacggccc gctttgattc atgaatctgg ccagggggtt ccgtccgagt
     5221 ccccggccaa gggcgaagac cagccagacg aagaccgcga gttcggcaag catcaggggc
     5281 agtttttgaa tttcaagccc cccggccggc cggatcgccc cgattatcag ggcgaagatc
     5341 gtccagccga agaggtcgtt tatcacggcg gcggtcatga tgacgtcgcc gatggggtcc
     5401 cgggtcagtt taagatccat caggattctg gcgatgatcg gaagggcgga aatggaaagc
     5461 gcggttccca tgaagaaggc gaaaagattg atgccgatac cgatgcgggg aagccacagc
     5521 cagtctaacg ccttgaccat ggccattccg aagacgaaag ggacgacgat tccgaacagg
     5581 ctggtaaaca aggccggccg gccgcttttt ttgaggccgg ccgggtttac ttcaagcccg
     5641 gcggtgaaca ggaagaagag catgccaagc ttgatcagcg tttcccgtac aaacgccacc
     5701 tgtcccgccg ccggaaagat cgtcgcgtga aaccccgggg caatcgctcc gaagacggtc
     5761 ggccccagaa tgattccgcc gagcagttct ccgaagacgg ccggctgacg gatttttttc
     5821 gccagctggc cgaaggccag ggcgaacgac agcattagga tcagctgcag gcagaaaagc
     5881 atgaaatcga gggagttcac ggccttttgt cctgtttgtt tcgaggggtt aaaaaccggg
     5941 ccagccgatc ttcttaatgc cgatccgtaa ccggccttgg ttctccccgg cgctgctccg
     6001 ggcggcgtac caggggagaa ggtcccggga acattttact tcggcgcgtt tctttcggca
     6061 agacggcaac gtacggaatc acggccgccc gacctccctt ttcccgctga cccttgacac
     6121 cctccggacc ggggagtata atcatatagc aattatgcta tttgctttaa ccggggacgc
     6181 atgaaggaac tggaagattt cctgcgggcg gtgggcgacg acaagcgcct ccgcatcctg
     6241 aagatgctgg aacggagacg cctctgcgtc tgcgaaatca ccgccgtgct tggcctccgg
     6301 cagccgaccg tctcccgcca ccttcggcgg ctggagaagg tggggctgat cgagcaggag
     6361 cagaacggct attaccggga gtgccggttg gtttccggcc atcctttcca tgatttgtgg
     6421 atgtggttga gcgaacggct tgaaaagagc gccgagatcc gggccgacgc gcggaagctg
     6481 gcgcgggtcg accggcgccg tctggcgtgc cggggcggct gatttttttt gacttgatgc
     6541 atagccgatt gactatttaa aaaccctgga ggcagtggtg atgaagaccc gtctgagcga
     6601 accggcgaaa cttctcctga tgctgtcgct cttccttttt tccttctacc tgccggttga
     6661 ctggctgccc ttccgcaacc cggtttacga ggccttcgcg ctcctgaagt ggtacgcgcg
     6721 cgaacacgtc ctgctctgcc tggtgccggc cttcttcatc gccggcgcca tctcggtctt
     6781 cgtcagccag gcggccgttc tcaagtattt cgggggcagg gcgaacaagt ggcttgccta
     6841 cggggtggcc tcggtttccg ggagtgtgct ggccgtctgc tcctgtaccg tcctgccgct
     6901 cttctccggc atctaccgcc ggggggccgg cctgggtccg gccaccgcct ttctctattc
     6961 cggccccgcc atcaatgtgc tggccatcat catgaccgcg cggatcctgg gctggcggct
     7021 tggcctggcc cgcgccgtcg gcgcggttct cttcagcgtc gttatcggcc tgctcatgca
     7081 cctgatcttt ttaaaagagg aacaacaccg tcaccaggct ggcgacttat tcaccgggga
     7141 cgaaacggag cgccgctcgc tccggcagga gaccctttat tttgccgccc tggttggtat
     7201 cctggtcttt gccaactggt cgaaaccgcc ggcggcgggt ctcggtccct gggcggtcgt
     7261 ttttcagctt aaatggttca tcaccggtct cctgggcctg gccctcctgg cgatgctgaa
     7321 ggcctggttc gcaaaggagg aattgaccgg atggatccag gcggcctgga cctttgccaa
     7381 gcagatcttt ccgctcctcc tggccggcgt tctcgtctcc gggctcttgc tcgggcgtcc
     7441 gggccacgaa ggcttgatac cgtcgcgatt cgtcgcggcg ctggtgggcg gcaactcgtt
     7501 tttcgcgaac ctgttcgcct cgatcgtggc ggccttcatg tactttgcca ccctgaccga
     7561 ggtgccgatt ctgcagggat tgatcggttc cggcatgggc caggggccgg ccctggccct
     7621 gctcctggcc ggcccggccc tcagcctgcc gagcatgctg gtcttgagaa gcgtgctggg
     7681 aacgaaaaaa acggttgttt acgtcctgct ggtcatcggg ttatccaccc tggccgggat
     7741 gctgtttggg gcattggccg ggtgatgatg gaggtcctgt tatgaaaaaa gaagccgggg
     7801 agatcaggcg gctggtccgg gagggctatg gccgggcggt caaacgaaag gcggccggcg
     7861 ccgcttccgc ttgcggctgc tgtaatgccg gggcggcgcc ggagcgaagc cggcgtgccg
     7921 gttacagcga caaggaaatg aaggccgttc ccggaggcgc caacctcggt ttcggctgcg
     7981 gcaacccgct ggccggatcg gagattaaaa gaggagaggt cgtgctcgat ctcggcagcg
     8041 gcgccggttt cgatgtcttc ctggcggccc ggctggtcgg gccggccggc cgcgtgatcg
     8101 gcgtggacat gacccctgca atggtcagga aggccgaaag gaacgcccgc cagggccgct
     8161 ttaccaacgt cgaattccga cggggcgaga tcgagaagct tccggtcgcg gatgccagcg
     8221 tcgacgtggt catttccaat tgcgtcatca atctttcccc cgagaaattg cgcgttttcc
     8281 gggaggcgtt tcgggtcttg aagcccggcg ggagattgat cgtttcggac ctggttttga
     8341 aaaagaggct cccggatttc ctgcggaact ccctggaggc ctacctgggc tgcctggccg
     8401 gcgccgttct ggagaacgcc tacctgaagc tgataaggaa agccgggttc gaggatgttg
     8461 aaatcacagg ccgttccggg tacccggttg atgattgctt cccggaggcc aggggcgccg
     8521 gagacttgat cagcagcttg acggtcaggg ccgttaaaaa aaactgaaag aaaggacgcg
     8581 attatgaaag tcgaaatcct gggtctgggc tgtccgaagt gcaaactttt gaccgccaat
     8641 gcggaaagcg ccgccaggga actcggtctc gaactggaga tcgtcaaggt caccgatatc
     8701 aggaagatct ccgagtacgg ggtcatgatg accccggccc tggttgttga cggggtcgtc
     8761 aggagcgccg gcaaggtcct gccggtcgag gcgatcaaga aactgctccg ctgattccgg
     8821 atggtttttt tgagaccggg gggggagagg gcatggaaga aaggaagcgg cgggtgctct
     8881 tcatctgcgt tcataacagc gcccggagcc agatggccga ggcattcctg aagcacctgg
     8941 ccggtgaccg gtttgaagtc gaaagcgccg gcctggaacc cggaattttg aatcccgatg
     9001 ccgtcgaggt gatgaaagag gtcggcctgg atatctcccg gaatcggacc aaaagtgttt
     9061 ttgaattatt gaaggcgggc cgttcttacg actacgtgat tacggtgtgc gacgaatcac
     9121 gggcgggaga gtgtccgatc tttcccggtc ccgcggcttc tcgatgccac tggactttcg
     9181 atgacccgtc cgcttttgaa ggaaccggcg ccgaaaaact ggagaagacc aggatcgtaa
     9241 gagaggcgat cagggaacgc ctggagcggt ggcttggaga aatcggcggc cgcgggtaac
     9301 ccgccgcgcc cggtttgccg ccgggccgtc cgtttggtgc gcgtcgaaag tctggttcga
     9361 ggatccggtc gcaaccatac atccgaaaaa ggagacttga aaatggccga cagcagaaag
     9421 cccagcctga cacggttcct ggcgtggcaa atccttgcca tcctccttat tgtcggaatt
     9481 gccgccgccc tgattttgaa taagcggcgg ccttcggtat cgccggccgc cgtcgctccg
     9541 gcgccggccg ccagttccgc aagcggaacg cccggggaaa ttcgccggga gaaaagcacg
     9601 gccgaccaaa aaccggcgcc cgggcccgcc gcgccggccg ccggttcgga accctccgcg
     9661 gccgcgcagg aggtcggcct cccccgactg ctcgaactgg gcgccgacag ctgtgttccc
     9721 tgcaagatga tggtgccggt cctggatgga ttgcgcaagg attacgccgg ccggttaaaa
     9781 gttgaattca tcgatgttta cgaggattcc cgggccgggg tttcacaccg ggtaagggtg
     9841 attccgaccc agatattctt cgatgcctcc gggaatgaac tctcccgcca cgagggattc
     9901 atgagccggg aagacatcct ggcgaagtgg ggaaagctgg gttacgattt tctcccggcg
     9961 gccggtgaat gatgtccgga atccttgctt tcctggggcg ggcgatcgaa ggcgctccgt
    10021 tggtcgcgct cctggcctcc gccgcctggg gcgttttgag ccttctcctg agtccctgcc
    10081 acctggccgc cattccgctt atcgtgggct ttatcgacga acagggccgg ctatccaggc
    10141 gccgggcctt tttgatatcg gttctttttg ctgccggcat cctgttgagc gtcacgatgg
    10201 tcggggtatt gaccgcggtg gccggaagga tgctgggcga tatcgggaga tacggcaatt
    10261 acttcgttgc ggccgttttt ttcatggccg gcctgcacct ggccggcctc ttcccgtttt
    10321 ctctttccgc cccgcaaggg ttgcccaccg gtaaaaaagg ggcggcggcg gcttttatgc
    10381 tcggtcttgt cttcgggatc gcgctgggac cctgcacctt cgcttacctg gctcccatcc
    10441 tgacggtcgc ctttaaaacg gcggccgcgg atttttacta tgggtttctg ctcctggtcg
    10501 cctatgggat cggccactcg gcggtcatcg tcctggccgg gacctttatc gggaccgttc
    10561 agcgttacct ggactggaac gaaaaatcaa aaggcgcccg gctcctcaag aaaacctgcg
    10621 gcctgctcgt aatcctcggc ggtctttacc tggtctatac ggcccgatga ccggattcca
    10681 cccgttctct ggttcggctc actcgaaacg cagggcgtag atatcggcgt ccttcattac
    10741 gaaacgcagc cggattggcg tgccggccag cccggccagt tctccgctgc cggtccaggc
    10801 ggccgttccc tctatttcgt tgccaagcaa ttccctggac tccttcagcg agaagcccgg
    10861 aaccggccgg ccgtcggcgg tctgaatctc cacccggatg ccgccggccg ccccggtcga
    10921 gtagttcaag cgcagttgcc ggccgctgaa ggtcagcacc cgggtaagca cttcgccgcc
    10981 gtcgtaaggg gcttgaatcg aggcgaagcc ggccagccgg agcgagtagc ggcgcatgtg
    11041 cgcgccgggt tgcgtataac tccggtggac atagaaagac atctcggccg ggccggtctg
    11101 gaccacgttc aaggccgggt aattggcgcg cgaagtccag ttctcaaacc cgatgggcgg
    11161 tcgtatgaag gcctccataa aggtgcggtc gtagaggttg ccgccgcgcg aggtcatgaa
    11221 gaccgcctcc gaacaatcct ttgatatcca ggccggggcc ggtttgtcgg cgccccggta
    11281 aatcggttcc agctgttccg gcgttacggc cgagcgtccc tgcatgaacc gggcggcgat
    11341 ggcgatgtag atgtgcggcg cccggaagta ggggtgggtc tggttggtgt agaggtgttc
    11401 aggcggcgcc gccttatcgt tgcaccgcgc ctccatcagt tccgccggcg tccactccag
    11461 gaagtccggg ctggtggtcc gggctatcca gcggtaggag cctcccaggg attcgcgcag
    11521 gtaacagacg tatttccctt ccgcctccga ccagaaggcg gagttctggg aatcgaaccg
    11581 gccctgccct ttcttgatca ccgcctcctg cagcgggtcc ttgttttccc ggatcttccg
    11641 ccaggcgatc ccgtcttccg aaacgtaggc gaagaggccc agggaccggt agccgcccag
    11701 ggccttgaaa cgttccccgg ccgggcatcc cggccgcgtg tcgagaaagg gcgtgaaatt
    11761 gttgcagacc gggagttggg tcatgaagac gatattattt ttctttgagc cggccgcctc
    11821 gtagaggccg agttccggct tggtccagtt gatgccgtcc ggggactcgg cgtagcagat
    11881 cgcctggttg ggatcggtat cacccttgtt gggctgggtc tgaacccggt agtagaagcg
    11941 atatttggat ccgtccttga tcaccgcggc atagatgccg tcgttgccct cccagggctg
    12001 gtcgaacttg aagatcattc cttcgtcccg cggctggtgc agaaccagcc gggcgccgtt
    12061 caggcgctcg atcaggtgat gatcgacgaa tatttcgcgc cgggtgccga gctcgatgac
    12121 ggattcgttc accttggcct cctgggcccg ggtggcggcc ggccaggtga tggccaggca
    12181 gatcatcccc aggcaaagca acgcgttctt ggcttccgga atcaggtgct tcatcttttc
    12241 tcctcggttc gcccctggtt atcggccggt tttcagctcc ggcttcgtaa cgctccttat
    12301 gtaatttatt tcttcagggt cgaagggcgt gccgtcggtc cggaagatgt cgtggaacca
    12361 gagcggcggt tccggtatcg gttccccggg ccggagccgg cggccggttt ttcgtaggat
    12421 ttctattttt cgggcggtct gccagttgaa atgggtctgg cttttaccgg cgaccaggcc
    12481 ccagttgagg caacccaccc ggtgttttct gaagatcggc aggctgtgct ggaaggtggt
    12541 gccgagttcc cgggccatgt actcggtgca gatgaccggc cggcctccgt tttcttgctt
    12601 ctgttcaacg atgatttttt caagcgtgcc gccgtcgtag ccgtgaaagg tgatcacgtc
    12661 cgaccggctc cggttgagga cgagattctt ttcgccccgg ctcccgttca gacaggccgt
    12721 caggggctgg acggccccgg cctgccgcgc ccaatcccag gccagttcga gcagttcgtt
    12781 cgaccggtcg ccgttgccgc cctgtcccgg ttcgttgtaa atgtcccaca tcaggacccg
    12841 ctcgtcgccg gcaaagcggg aaatgacgcc ccggacgtat tcagacagcc gctgcttctc
    12901 cccttccggc gccgtgccct cgttaaaccg ccggacgatt tcctgccccg ggctctgctt
    12961 ccactccgag ttatgcaccc cggccagggg ttggggctgg ggaccgctgg ccgggtccgg
    13021 ccggtggcag tcatcgaaaa gcaccggtat gacccgcagg ccgcgccgcc gggccctctc
    13081 cagaaaatcg tcgattcttt cggcgcaacc cgtccggtcg tcagcccagg ccaggtcgtg
    13141 caggtaaacg cgcagcgtgt tgaatcccag gccttccgcc cagctcaatt cgcggtcgat
    13201 cgtcaccgga tcgtatgttt cggcctgcca catctccagc tggttgaccg cgttgctggg
    13261 tagaaagttg cagcccacca gccagggctg ttcccggtac caggcggccg ccttttccac
    13321 gctccatctc ttcatgcttg gtttccgggg aggcccggtt tgatgtggaa tttgttcgat
    13381 ttctccttgg tttccagtaa attttgccac cggccggccg gtttgtcaac cgggccaaaa
    13441 gaagtttttt ggggatggcc tgactttttc agaagcccgc cttccagggt ttttcggccg
    13501 gggcggatac gccgcccggc cgaacgtctt aaccgtggcc gggatttatt tagaaccatt
    13561 tgattaacag cggtttcctg ccgaagatga gatacctggg agacactccg gattccttat
    13621 cgcatggtag gattaagggg ttttgaaaag gaccaaagga gtggtccccg gataccttgt
    13681 ccaggcagcc agttgacagg gactggcgaa cccaggtata aatattataa aatacagcaa
    13741 attgccggga acggcggagt cgatagccag gggtgttttt ttgttcattt caaggggggg
    13801 gcgcatgctt gaaaaaaaag gatggggcgg gctggcattc tttttgatgg gtttcttgct
    13861 gctggccgtt caggcccggg cccaggttac gcccggcacc taccagcgga ttgacggcaa
    13921 cccgcttcgg atcgacgcct acgaggacgg aacgttgggg gtttaccgct ggaatggcac
    13981 cgattacatc aatcagtatt acagtggtaa ttgctggggt tcggtactct tactgcattc
    14041 gccccggacc actgacgcct tcaacagctg gtactttatt tactggggtt acaccaacgc
    14101 caagccgcag ttcacgccgg tcagcaacac caagccggat ccctggacga cggttaccgt
    14161 cttccgggcc ggcgactcgg gggtgcgcct cacccagacc atcaaataca ccgacggcaa
    14221 cgcctactac aagaagagct ggcggatcga gaatctcggc agcaccgaat ataacgattg
    14281 ccggttcttc cacggcggcg acagtacttt cggtggaata gacgcctcgc ggggccactg
    14341 ggacgccaac ctgggcatgg tctatctcac caaccccgac caggcgatcg atggcatcat
    14401 gggcttctac ggcgacctca ccactccggc cacctcttat gctgaggggg agtacgacga
    14461 tgaggtttgc gagcggatac tggacacagg gcaactcggt aattcggtga accccggtta
    14521 tattgacgcc ggctacgcct tgcagtggaa ccggggcacc ctgtcgcccg gtgacgtctg
    14581 gaccatcatc tcctacgaaa aatggaccga ggccggcttc gtccaggtct tcgccccggc
    14641 cgagcgggag gtcgaggcgg gcgatgtcat ccagcaccag ttcgtggtcg ccaattacca
    14701 ggccacgacc gacaccttca tcctgcagcc ggactcttct ctggattggc cctgggcctt
    14761 tgtcaatccc gcccaggccc aggttaccct ggcggccggc gccagcacca cggtctcttt
    14821 tcaggttacc atcccggcgg gcgcggcccc cgggacccgg gacgtcctga cgctgacggc
    14881 gacctcccag accacgccgg cagtcaccaa ccaggatagc gtcgccacag tcattcccgg
    14941 cgttctggcg ggaatcgttt ccaagctgca gggcggcggc gggccctgct tcatcgccac
    15001 cgccgcctac ggttcctacc aggagccgca cgtccaggtc ctgcgccggt tccgggaccg
    15061 gttcctgctg gccagcccgc cgggccgggc cttcgtccgt ttctactacc gccattcgcc
    15121 gcccctggcg gcggtgattg cccggaacga aacgctccgg gccgcgaccc ggacggccct
    15181 gctgcctgtt tacggcgtcg cctggctctg cctgaacggg cgatcggtcc tggccggact
    15241 cctgggctcg gtcctgctgg ccgggctgtt cctgctggcc ggccggcgcc ggttccgctt
    15301 ggcggccgga gtgacggccg ttatcctgtt gctgggcgcc gttccggcct cggccctcga
    15361 catcaacaac ttcagcccgg ccatcggcgg cgattccttc accctgcttc ccgcctccaa
    15421 ggtactgccg gcccggaatt tttcggctga tctctacctg gtttatgtcg aaaagcccct
    15481 gcgtggaaat gtcgggggca agtggacggt actctccgag gaccagacgc tggccacgct
    15541 cggcctgggc ctgggggtga ccgactcgtt tcaactcggc ctgcggcttc cctatctctt
    15601 cagccagggc agccaactta ccggcgcggc ggccccgggc gacggtgata tcggcgacct
    15661 gcgcctggag ggaaagtacc gggtccgcgg cggtccagac caggttggtg tcgccctgct
    15721 gccctatgta accctggaaa ccggcgagga ggacagctac ttcggaaagg gttccaacgc
    15781 cttcgggctg acggcggtcc tggatcggaa tttcggcgac cgggtctgcc tggccttcaa
    15841 ggtcggctac cagtaccagg agaaggaaaa tctgcccgat ttcgacctcg aagatacgct
    15901 cctctttggc gccgggatcg gctggaaact ccccaacgac cggacccggg cctcggtgga
    15961 actccagggg ctcacgaacg cctccgccct cttcaacagc gaggatgcca ccccggtgga
    16021 gctcctgggc tccatccacc atcccgtcaa tgagaatctt ctgctggacc tgggcctcgg
    16081 cgtcggcctg accgacggct acggcgcccc ggaataccgg cttttcctgg gaatgcgcac
    16141 cggtttttag tccgcggttc cggccgcgtc tcaccgcggg tgtggccggt tctctttgaa
    16201 acaacaaggc ccggccaggc ctggccgggc cttcattttg accggttgcc ggatcggttt
    16261 tcttggtccg gtggcttggg aagagttttc tcctgacacc ggtgattttc accaactgga
    16321 gaggggaaat atggaaatga atgtctttga agccattcct tatgccgacg aacccttcgg
    16381 ccgccgaaaa gtagtcgacc agaagcacct gctggtgatg cagatcgcgc ttaagcccgg
    16441 ccaggcggtt cctccgcacg aggcgaactc caacgtgcat ctcctggttc tggacgggag
    16501 cgtggttgtc tccctctccg gtcaggatac cctggcccgg cgcggggata tcctgccggt
    16561 cgccttcagg acgccgatgc ggatccggaa cgagtccggg gcgaacgcga ctttcctcgt
    16621 tatcaagacg cccaacccga gcgaaatggg cgggtgaatc cggaggcacc aatgaagcgg
    16681 attcaagtga aacgggttta tgagccggcc gggccggcgg acggggtgcg ggttttggtc
    16741 gaccggcttt ggccgcgggg attgagcaag gcgaaggcgg ccgtaaacga gtggctgaag
    16801 gaggccgcgc cttctcaggt cctgcgccag tggttctccc acgatccccg gaaatggccg
    16861 gagttccgca agaagtattt cgaggagctc gattcccggc ccgaggtggt tttcaaactg
    16921 cggcagctgc tccggaaagg tccggcgacc ctgctctacg gagcccggga cgaaaacttc
    16981 aacaacgcgg ttgccctcgc ggaatatctc aaaaagaaag ccggataaaa atgcccggcg
    17041 catctttacc cggcttatcc cgcccttatc ccatcccggt ttaaataatc gcccccggcc
    17101 ggtccgtccg gtctccgggg ccctctcttt ttcagcgcag gttcttcagc tcgagtccgg
    17161 tcgtgaagat gatccgcttg acgccttccg gcggcgtggt gagatgaaag cggctttcca
    17221 gcccggcctc gccattgcgc acgaagaact ggaagcccgg cggggtcaag agcttgatgt
    17281 taggatcggg cgccggagcg aacagggaga ctcggtaggc gtccgcgatc tccaggatgg
    17341 ccgtgccctc cttcagggag aagccggccg gaacggccgc cggtccggtg ggcgtcagct
    17401 tgccgaaatc gtgggcgatc tgtcccccga ggccggtgac cggcccggcc gccggccggt
    17461 aatagtaggc gtaattccgg tccaggatat actcgcccag catcagccgg acaaaaccct
    17521 cttttgggga gtccccgcgc caggaatcgg cccgcaccgt gaaggtcagg ccgtcctggt
    17581 cggccatcag ggcaaggtat ttccggccgg gcgggttgta ggggtcgtcg atccggaccg
    17641 aggtgacgat cccgttgcct tccgcgctta tctgcatccg gtccccgaag ggcacgtcgg
    17701 tctggattcc gttgatgacc ggttcgagcc ggttgccttt cttcaagaaa accttgagaa
    17761 tccctttgtc gttctggtca acgtgaacca cgatcggtcc ggtttcgaac cgtttgaagg
    17821 agggctgtgc ctcccgggcc agtttcaccg tctcgagtcg ggccaggaag gcctccagcc
    17881 cggccgccgg cagggtgacg tacacgggca gtaccggtac cgtgacctcc agccgctccc
    17941 cgttctgctt cggcttcaat tcgttggcga agagatcgta aaacctggtt ccttgcgggg
    18001 cgggcaggct cagggtatgc ggcttgtcga gatcggcctc actggacgtc atgaag
//
LOCUS       DAOQYN010000012        17978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_3514_length_17978_cov_4.553311, whole genome shotgun sequence.
ACCESSION   DAOQYN010000012 DAOQYN010000000
VERSION     DAOQYN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17978
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3514_length_17978_cov_4.553311"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PKN80_01095"
     CDS             complement(<1..776)
                     /locus_tag="PKN80_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ34644.1"
                     /translation="MSTPIIDFHTHAFPDELAGRAIAHLEAEGGIKARLDGRVSSLLA
                     AMDRTGISTSVVCSIATRPGQFDSILAWSRRIASERIIPFPSIHPADPDYREHLKKVA
                     GAGFKGIKLHPYYQDFDLDSPVLFPVYEELCRHGLILQAHTGFDFAFEYVDRCGPARI
                     MEVVRRYPELKLVATHLGAWKQWDEVEDLLAGHPVYLEISFSLEFLDREQALRILSRH
                     DPGRILYGSDSPWTDQQTTLELFQGLELGPELDAAVLAGNA"
     gene            complement(815..1240)
                     /locus_tag="PKN80_01100"
     CDS             complement(815..1240)
                     /locus_tag="PKN80_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34645.1"
                     /translation="MNKRDLLTAVFFGTIILSAPPSLQARSKLNALPGREKVTVNLAN
                     PAATLVEEERVLTLQEGLNQVDFSWKGVNIDPDSLRLTILGRNFPHQYWELNRDEADL
                     RYEVVDLDTARFILDLPPNTTREFFYDLRLYEGVRRDRR"
     gene            complement(1494..1646)
                     /locus_tag="PKN80_01105"
     CDS             complement(1494..1646)
                     /locus_tag="PKN80_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34646.1"
                     /translation="MRRKLGIGLTAVSLAVMMLLSGFVSAAEKSEGTQPMKCGSCCCK
                     SSCMGN"
     gene            1821..3530
                     /locus_tag="PKN80_01110"
     CDS             1821..3530
                     /locus_tag="PKN80_01110"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNQ34647.1"
                     /translation="MIRIALAQINPTVGDIPGNTARIIAGLKEARRAGARLAVFPEMA
                     VTGYPPEDLVFKPAFIQANLEALEKIARQADGLAAVVGFIDRGDDIYNAAAVIDREGI
                     RAVYHKHLLPNYSVFDEERYFKAGQNNALFQLGELRFAVNICEDIWHPSGPMAEQAAA
                     GSQLIVNISASPYQYGKVEYKERMLAGRAADYSVFLAYVNLVGGQDELVFDGQSLVFD
                     PKGQLLARARPFETDLLVVDLEIERILKRRIQEPRLRKTPSRSVPLVTLSAAPPARVK
                     TAAPRIEAHLPETAEVYRALVTGTRDYVEKNGFRKVLIGLSGGIDSALTAAVAVDALG
                     KERVVGLYMPGPYSARISGEDSRKLAENLGIEFDQVPIRAIFRTYLETLRKPFAGRKP
                     DAAEENIQARIRGNLIMAFSNKFGYLVLTTGNKSEIATGYCTLYGDMAGGFAVLKDIP
                     KTLVYRLAEYRNGQRPDIPERSLTRPPTAELRPNQKDSDSLPPYERLDPILKLYIEEN
                     LAPAEIAGGGTYREAEVRAIVEKVDRNEYKRRQAPPGVKITGRAFGKDWRLPITNRFR
                     NGG"
     gene            complement(3649..3852)
                     /locus_tag="PKN80_01115"
                     /pseudo
     CDS             complement(3649..3852)
                     /locus_tag="PKN80_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019602620.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            complement(3833..5164)
                     /gene="xseA"
                     /locus_tag="PKN80_01120"
     CDS             complement(3833..5164)
                     /gene="xseA"
                     /locus_tag="PKN80_01120"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNQ34648.1"
                     /translation="MGEDLFQEKTEGSGSPTERIYTVTELSQAVRHRLESAFPLVWVE
                     GEVSSLRQPISGHLYFSLKDAGSQLRVVLFRGSAARLRFKPADGLQVLVFGRVSTYAD
                     RSEYQVIAQLVEPRGLGALQLEFERLKKKLAAEGLFAPERKRPIPVLIKRLGVVTSPT
                     GAAVRDILKVLKRRNPAIEVVIYPALVQGKGAAAEIVRGIMELNRLGGFDALLVTRGG
                     GSLEDLWAFNEEPVARAIAESLVPVISAVGHEVDYTISDFVADLRAPTPSAAAELISL
                     HRAETVERIGQLADRLAYLMRSRLAELDRRLKSITGRYGFQRPQELLATLTQRLDDLY
                     ERLARGQRNQLSGAGQRWEALARRLDGLSPLAVLDRGYSITLTYPEGRVVKEASLLPP
                     GRLIRSRFLKGEVVSRVVRPAGAGRSGPEPPEQPGLFESDSGGDEDGQDQG"
     gene            complement(5175..6047)
                     /locus_tag="PKN80_01125"
     CDS             complement(5175..6047)
                     /locus_tag="PKN80_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNQ34649.1"
                     /translation="MERLAIGRRNIIWALAINLALFAFKFYAGLAGMSQAMVTDAFHT
                     LSDILATSIVFFGLMLGGRPSDPDHPYGHGKFETMAAGACSLFLVAAGFLFIYEAVLV
                     LLTRHFHVPTFLPLLAAVFSIGLKEFLYQYTIRVARRINSTALRADAWHHRSDAFSSI
                     PAFLGILGARLGLPFLDPLAAILVAVFVIHVGLVIGQDVFSELVESSVDGPTLARIRA
                     VAGSVEGVRHIHDLGTRMLGPSIIVDLHILVDGNLSVQRGHEIADRVEHRLKEEVPAV
                     NRVTVHVDPIGRLT"
     gene            complement(6146..6919)
                     /locus_tag="PKN80_01130"
     CDS             complement(6146..6919)
                     /locus_tag="PKN80_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNQ34650.1"
                     /translation="MNNRGRINWLGLLLVLFTMVPWLSGCTTVYNPVTGKSESTLYSS
                     QDELSMGKAVDAQIKKEYKASQDQALLARIKTIGDRVAVNAGRSDIPFTFQVLETDQV
                     NAFAGPGGYVYVTTGLLKLVGSDDELACVLGHEIGHVAARHAVKQLQSQVFYSLSASI
                     IFSEERYAEIEKAVNLAFNLRQLSYSRKDELQADELGMQYGLKSGYKPEGMLSFLKKL
                     AEIEKKQPRLIIVPLSTHPVTSVRIENATAWIESKRRSA"
     gene            complement(7011..10121)
                     /locus_tag="PKN80_01135"
     CDS             complement(7011..10121)
                     /locus_tag="PKN80_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNQ34651.1"
                     /translation="MNLPELGVKRPVLTSMLFLALIILGLVSLGRLGMDLLPDIEIPS
                     VAVVTTYQGAGPQEVETQITRPIEKVMATVSGLDKIESSSSQDISLVQLRFEWGVNLE
                     DKANEIRDKIGQVMPMLPDAAERPFVIKFDFSMMPVVMVGVEAGSSYSWIYEYLDKNV
                     SRQLEQLPGVASVSIYGGNRREIQVAVDRRRLEAYQVSLRQVSAAIRSANLNEPGGFL
                     KSGAYDFILRTPEELTIPQLNRVVVTQRAGRPVYLSDLATVSDGYAEKVGGVTVSDRP
                     GLFMIVQKQSGVNTVEVANRVKLEMARIQKELPPDVHFRTLFDQADFIRRSINNLRDS
                     LLQGGLLVILVIFFFVRDLRSSFIVAISIPVSLITTFFMMYGAGFSLNMISLSSLGIA
                     VGMVVDAAIVVFENILRHREAGEGPIESALNGAQEVFGAVVASVLAVVTVFVPIIFTG
                     GVSGILFKQLALSFSFCLLCSLLTAVMLIPLLSSRLLRTGGSGRGIQAAVLESGERFF
                     RNLSAGYRNLLDWTLNHKKISLGFSCLMLLGSLLLVPLVGTEFMPEMDQGMISFSVEM
                     PVGTRQEVTGEMIRKLTDILARNVPEAEVIMGHWGRMSGNSAMVAMSGGKPPAGYRGE
                     LIIKLISKEKRQRSGRQIAESLKPLLKAPPARVLFDASDPMASMLFGSAKPLTVEIKG
                     YDLEKSRQLAAEVSRVLGGIPGVSDVSLSRDEGQPEYQIKVRRDKSSLMGLSVSDISS
                     QVKSYYAGDTGNQYRESGEQYDIMIRLRDSDRADPEVLAGVYLANPGGGLVRLSQVAD
                     IRAGTGPVSISRRDQERYVEVSGEIYNRSLSSVVNEAEPQLRRLSLPSGFSLAFTGGR
                     EEQVKSFRVMLFALILSVVLVYMVMASQFESFLDPFVIMFAVPFSLIGVIWSLFLTGN
                     TLSLTSFLGLIMLVGVVEETGIILVTFAIELRKKGLPLREALLQAGQLRLRPILMTSL
                     TTIMGMVPLVIARGEGSEMFRPLAIVIIGGLLVAMLMTLFFIPVLYFLLEERVKKPSG
                     APPRPR"
     gene            complement(10118..11206)
                     /locus_tag="PKN80_01140"
     CDS             complement(10118..11206)
                     /locus_tag="PKN80_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNQ34652.1"
                     /translation="MKRRKLLLFFLAVIVGLVGWRVQQRLSVKPPAREAETLNVTLQP
                     VARRNLTEELSFSGTLEASRAVPILARVSGKLEEVLFQEGDWVKPGQIVARIETRDYQ
                     LRLLEARAARLAAAAELENAGKNFGRMESLFREEVITAQEMDNARARRDAAAAAVERF
                     EVQIKMVQEQLADCLITAPFGGFVSRRFFDPGVFIGAGSGPILELVDLAELKVKIPVG
                     ETDLARLRLGQPVKIVADAFPDLSVSGRVTRLSPVLDPVTRTAGCEIAIANPGGRFKA
                     GMFVRAEVLVGSHQGVLTISQEALSLQGDNPTVYVVKDGTASLRQVKLGFQSGPLFEV
                     TGGLAEGEKVVISGFGSLYDGAKVEISE"
     gene            complement(11244..12542)
                     /locus_tag="PKN80_01145"
     CDS             complement(11244..12542)
                     /locus_tag="PKN80_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNQ34653.1"
                     /translation="MSPKMRISRLVFVLFGLIISPLFHGAAAGAETLTLEKAVAEARS
                     KSLLLQQAQAGLASASYAAAEQKTYLYPSFNLSGNYGYWEGLSSAGSSNGQDSASLAF
                     SATQPIYNGGRLKAAYQSALLNVGLARHVYDQAGLDLALQVRNSYYTILRLKKTVSVA
                     DELVANRQRHLAEVEKKVQIGVLPKVEKLKAEVELAHSRDSLTSVRNNLALAYSSLNR
                     LLERPLEGALDLAEVDTTARPNWSGITPEVLYEEALKQRPEIRYGELQLARSQQNEIL
                     ARSGYHPELALTLSQGESQADAFRGSWQDSSQLMLVFSWNVWNFGQVRNRLAASRSET
                     SRVGLALDDQVRMVRLEVKNAWLSLESSLDRIDTAGQAVGQAEEVLRMQGIRFQEGLA
                     TNTEVLDAEFALAQARTNYYNAIYDFLQAEAYLRRSLGRE"
     gene            12696..13349
                     /locus_tag="PKN80_01150"
     CDS             12696..13349
                     /locus_tag="PKN80_01150"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ34654.1"
                     /translation="MIKKKSNSRKSGPPSEKSGAILKAAKRLFLGRRFDRVLMDDIAR
                     AAGCAKGTLYLYFRTKEDLYLGILTRDFDLFHRRLESSLRNPGTAWEALGKITALLFR
                     FLKGNYDFFVLSRQAEIHLRRKHRELLREQHNRIIQMLAGLVGKGMEEGVIQNGDPEG
                     YAHILLGMIFASAHPASRPAAQTPKLVLRIFAEGIRSRPDRGPTRTSSRTGSPRRPG"
     gene            complement(13303..13779)
                     /locus_tag="PKN80_01155"
     CDS             complement(13303..13779)
                     /locus_tag="PKN80_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34655.1"
                     /translation="MDGKNGSRPARPKFKRSFWRIINKPVQIRYSLVVVWLIILALLL
                     GGLVTYLTVWNLLLRIPIPDPAALEHLQGQVLRLLVLEFLIGGAVLLFLAVLLQLRIM
                     HRICGPLFRLEEVMNQLAAGKLPPYPLLFREKDYFYDLARAFNRAVEAIRSGRKFE"
     gene            complement(13829..14587)
                     /locus_tag="PKN80_01160"
     CDS             complement(13829..14587)
                     /locus_tag="PKN80_01160"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNQ34656.1"
                     /translation="MDARTRRESSFTIIELVVGLLVLFLLSAIGFSLIRAASQKTKIA
                     VAKAYISQYASLVDGIRNDLGYYPPSVNETLESLTYRDSPFPGRRGWQGPYLKTIPID
                     PWKTPYFYCVTNGVVFGPQQCRREPGGQPYEETFTFRTPPTGQGGMIIHNFQVASGRI
                     ILNGVEIFHPNEFNPNLDHMEKTVALDKVNTLYVWIPSNVRPHNYFLVSITSPFSRAT
                     TYILGSRGADGQPGGAEFEEDLVWIEGQATGSFK"
     gene            complement(14609..15082)
                     /locus_tag="PKN80_01165"
     CDS             complement(14609..15082)
                     /locus_tag="PKN80_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34657.1"
                     /translation="MDQKNEVKSRPKFKRSFWRIINKTVQIRYSLVVIWLIIIALMLG
                     GMVTYLTVWNLLLEIPIHDPAALEQLQRQVLRLLTLEFLIGGVVLVVLAGVLELRILH
                     RICGPLFRLEQLMGQLAEGKLPKHPLVFREKDFFYNLAESFNRAVEAIRSGKKFE"
     gene            complement(15113..15874)
                     /locus_tag="PKN80_01170"
     CDS             complement(15113..15874)
                     /locus_tag="PKN80_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNQ34658.1"
                     /translation="MPNGLKRKNNGFTLIELAVGLVILFLLAGLGFSLIRSASQKTKV
                     AVAKTYIAQFAMVIDGIRNDCGFYPPSENGNLESLTSAGPPSPKFRGWQGPYFKTLPR
                     DPWRNDYFYLIQNGLVFGPQECRRQQRGGPYDETFVFDSTATGNAGLIIENFLVASAR
                     VWLNGMEIFHPNQFNPQVSHLEAAVTLLARNSLRVWIPSNVQHSNYFLISVTSPLSRK
                     ASYILGSRGADKQPGGDGFAADLVWLPGQATGSFR"
     gene            complement(15894..17285)
                     /gene="murC"
                     /locus_tag="PKN80_01175"
     CDS             complement(15894..17285)
                     /gene="murC"
                     /locus_tag="PKN80_01175"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNQ34659.1"
                     /translation="MGEILVDLERLERVHFIGIGGIGTSGVARILLGEGKRVTGSDLG
                     DNLLLEQMRRDGARILIGHRADNLPEDAQVVIISAAIPHDNPEILAAQALSIPIITYS
                     QALGSLMRRRFGIAVAGTHGKTSTSAMVAVILKTAGLDPSFVIGGEIPLLGGNSGTGQ
                     GRYLVAEACEFRRNFLSLRPRVALVTNIEEDHLDYYKDIEEIEEAFLQFLSRLPTDGF
                     AVVCRESANVQEVVSSLDRPVYTYGFSGADFRAESMELRPDSSAYRAFYREECLGTVT
                     IAVPGIHNILNSLGAFATCRLLGLEPEMIIRGLAAFRGVNRRLEKKGIYRGAVVLDDY
                     GHHPTEIVATLAAIRQFYPAGRLLVVFQPHQYSRTRFFLEDFARSFRLADEVIVPEIY
                     FVRDSEESRRSVSAADLVERLRANGKKAGFFDSFARVVDYLRENLVAGDVLLTLGAGP
                     VHQVGEELLQSEP"
     gene            complement(17414..>17978)
                     /locus_tag="PKN80_01180"
     CDS             complement(17414..>17978)
                     /locus_tag="PKN80_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNQ34660.1"
                     /translation="QCGLADRVITEISAAGAGAFRMNGRRPGLVVDIGGQDTKVIEIN
                     GEGSVSDFLMNDKCAAGTGRFLELMAQVLETDMEGFSQLARSAEAPRRLNATCSVFAE
                     SEVVSLIAHGESKTNIAAGLFEAVASRVAAMLRQFQEQPVFFCGGGARSPALRLALER
                     LAGRPVQVLDPPQFVVAFGAAMLPDGV"
BASE COUNT         3436 a         5688 c         5803 g         3051 t
ORIGIN      
        1 gcgttgccgg ccaggacggc cgcatcgagc tccgggccca gttccaggcc ctggaagagt
       61 tcgagggtgg tctgctggtc ggtccagggc gaatcgcttc cgtaaaggat ccggcccgga
      121 tcgtgcctgg aaaggatgcg cagcgcctgt tcccggtcga ggaactcgag tgagaaggag
      181 atctccaggt aaaccgggtg gccggccagg agatcctcca cctcgtccca ctgcttccag
      241 gcgcccaggt gggtggcgac cagtttcagt tccggatagc gccggaccac ttccatgatc
      301 cgggccggac cgcagcggtc cacgtattcg aaggcgaaat cgaaaccggt gtgggcctgg
      361 aggatgagcc cgtggcggca gagctcctcg tagaccggga aaagaacggg cgaatccaga
      421 tcgaagtcct ggtagtaggg atgcagcttg atccccttga agcccgcccc ggccactttc
      481 ttgagatgct cccggtaatc cgggtcggcc gggtgaatgg aaggaaaggg aatgatgcgt
      541 tcggaggcga tccggcggct ccaggccagg atggagtcga actggcccgg ccgggtggcg
      601 atgctgcaga cgacgctggt gctgatgccg gtccgatcca tggccgccag cagggacgag
      661 acccggccgt ccaggcgggc cttgatgcca ccctccgcct cgaggtgggc gatggcccgg
      721 ccggccagct cgtccggaaa ggcgtgggtg tgaaaatcga ttatcggtgt gctcatggcc
      781 cggtcagggt tgggagcgtc cgccgggggc cggctcaacg ccggtcgcgg cggacgcctt
      841 cgtaaaggcg gaggtcgtag aagaactcgc gggtagtgtt gggcggcaga tccaggatga
      901 accgggcggt gtccagatcg accacttcat acctgagatc ggcctcgtcg cggttcaact
      961 cccagtactg gtgcggaaag ttccggccca ggatggtcag ccggaggctg tccggatcga
     1021 tgttgacgcc cttccaggag aaatcaacct ggttcagccc ttcctgaagg gtgagcaccc
     1081 gctcctcctc gaccagggtg gcggccgggt tggccaggtt cacggtcacc ttctcccggc
     1141 cgggcagcgc gttgagcttg ctccgggcct ggagggacgg cggtgccgaa agaatgatcg
     1201 tcccgaaaaa gacagcggtt aaaagatccc ttttattcat aagccataag cattttaccc
     1261 cgtaccaacc ggcctgtcaa tatcaataaa aaaagggtgg gacagcggcc cggactcttt
     1321 taaaaaattg agggacaggc ccggaaccgg tgaaatatca acggtccggc gggagaccca
     1381 aacgaatggc ggcgccggca aaaaaagggc ggctcttccg ggaccagccg gccgactgga
     1441 gatccggaag aaccgcccga acatcagaac cagggaagca agccgcttcc ggatcagttg
     1501 cccatgcagg acgatttgca gcagcaggat ccgcacttca tcggctgcgt tccctcgctc
     1561 ttctcggccg cggaaacgaa acccgacagg agcatcatca ccgccaggga gaccgcggtc
     1621 aagccaatac ccagcttcct tctcatcgtt gatcaccccc tttccattgt taatcaatca
     1681 aaatcttcat acttactatt actatcgtat tagtaatgat tttatcggaa aacgtttaac
     1741 cggtcaagtg ctgactctct ctacattgaa agcggcccga tattgccagg gcaggcgcct
     1801 ttggggtaaa atctccccat atgataagga tcgccctggc ccagatcaat cccaccgtcg
     1861 gcgacatccc gggaaatacc gccaggatca tcgccggact caaggaggcg cggcgggccg
     1921 gcgcaaggct ggcggtcttc ccggagatgg ccgtcaccgg ctacccgccc gaggacctgg
     1981 tattcaaacc ggccttcatc caggccaacc tggaggcgct ggaaaaaatc gcccgccagg
     2041 cggacggcct ggcggcggtg gtcggtttca ttgaccgcgg ggatgatatt tacaacgcgg
     2101 cggcggtcat tgaccgggag ggaatccggg ccgtctacca caagcacctg ctgcccaact
     2161 acagcgtctt cgacgaggag cgctacttca aggccggcca gaacaacgcg ctcttccagc
     2221 tcggtgagct ccgtttcgcc gtcaatatct gcgaagacat ctggcaccct tccggaccga
     2281 tggccgaaca ggcggcggcc ggcagccagc tgatcgtcaa catctccgcc tcgccctacc
     2341 agtatggcaa ggtggagtac aaggaacgga tgctggccgg ccgggccgcc gattactcgg
     2401 tcttcctggc ctatgtcaac ctggtcggcg gccaggacga actggtcttc gacggccaga
     2461 gcctggtctt cgatccgaag gggcagctgc tggcccgggc gcgccccttc gagacggacc
     2521 tgctggtggt tgacctggaa atcgaacgca tcctcaagcg gcgcatccag gagccgcgcc
     2581 tgcgcaaaac cccgtcccgg agcgtcccgc tggtgactct ttcggcggcg ccgccggcca
     2641 gggtcaaaac agccgcgccc cggatcgagg cgcacctgcc ggaaacggcc gaggtctacc
     2701 gggcgctggt aaccggcacc cgggattacg tcgaaaagaa cggcttccgg aaggtgctga
     2761 tcggcctctc gggcgggatc gactcggcac tgacggccgc cgtcgcggtg gacgcgctgg
     2821 gaaaggaacg ggtggtcggc ctctacatgc ccggccccta ctcggcccgg atcagcgggg
     2881 aggacagccg gaagctggcg gagaacctgg gcatcgagtt cgaccaggtc ccgatccggg
     2941 cgatcttcag aacctacctg gaaaccctgc gaaagccctt cgccggccgg aagccggacg
     3001 cggccgagga gaacatccag gcccgcatcc ggggcaacct gatcatggcc ttctccaaca
     3061 agttcggcta cctggtgctg accaccggca acaagagcga gatcgccacc ggttactgca
     3121 ccctttacgg ggacatggcc ggggggttcg cggtcctcaa ggacattccc aagaccctgg
     3181 tctaccggct ggccgaatac cgcaacggac agcggccgga tatcccggaa cggagcctga
     3241 cccggccgcc caccgccgaa ttgcgcccca accagaagga cagcgactcg cttccccctt
     3301 acgaacggct ggacccgatc ctgaagcttt acatcgagga gaacctggcg ccggccgaga
     3361 tcgccggcgg cgggacttac cgcgaggcgg aggtgcgggc gatcgtggag aaagtggacc
     3421 ggaacgagta caagcgccgg caggcgccgc cgggagtcaa gatcaccggc cgggccttcg
     3481 gcaaggactg gcggctgccg atcaccaacc ggttccgcaa cgggggctga ctattaatcc
     3541 aggcggcggt cactggccgg cggccgcctc cgcgtcgtcc tcttccgtct ccaactcggg
     3601 cgactccgat tccgggccgg ccgggaatga ttcgcggccg gtaacccggc cctcctggtt
     3661 cttggtcagc agctccacct tctgctgggc ggccttcaat ttctccccgg cgaagcggac
     3721 caggccgatg ccctcctcgt aaagcttcag ggaatcatcc aggccgatat tgcccgattc
     3781 cagtcccttg atgatctcct ccagccgttc cagcgcgctc tcgaacttga tcttatcctt
     3841 ggtcctggcc atcttcatcc cctcccgaat ccgattcaaa caggcccggt tgttccggcg
     3901 gctccggtcc ggaccgaccc gctccggccg gccggaccac ccggctcacc acctcaccct
     3961 tcaggaagcg gctccggatc agccggcccg gcggcaacag cgacgcctcc ttgaccaccc
     4021 tcccctccgg gtaggtgagg gtgatgctgt agccccggtc caggaccgcc agcggagaaa
     4081 gcccgtccag gcggcgggcg agcgcctccc agcgctgtcc ggcgccggac agttgattgc
     4141 gctggccgcg cgccagccgc tcatagagat cgtccaggcg ctgggtcagc gtggccagca
     4201 gttcctgtgg acgctgaaac ccgtaacggc cggtgatgct cttcagccgc cggtccagct
     4261 cggccaggcg gctgcgcatc aggtaggcga ggcggtcggc cagctggccg atccgttcca
     4321 ccgtttcggc ccggtgcagc gagatcagct cggcagcggc cgaaggcgtg ggcgcccgca
     4381 ggtcggccac gaaatcactg atggtgtaat caacctcgtg gccgaccgcc gagatcaccg
     4441 gaaccagtga ttcggcgatg gcgcgggcca ccggctcctc gttgaaggcc cagaggtctt
     4501 ccagactgcc gccgccccgg gtgaccagca gggcgtcgaa accgcccagc cggttcagct
     4561 ccatgatgcc ccggacgatc tcggcggccg cgcccttgcc ctggaccagg gccgggtaga
     4621 tgaccacctc gatggccgga ttgcgccgct tgagcacctt caggatgtcg cgcacggcgg
     4681 ccccggtggg cgaggtgacc acgcccagcc gcttgatcag caccggtatc ggccgcttcc
     4741 gttccggggc gaagagcccc tcggcggcca gcttcttctt gagccgctcg aactccagct
     4801 ggagggcacc caggccgcgg ggctcgacca gctgggcgat gacctggtac tcgctgcggt
     4861 cggcgtaggt ggagacccgg ccgaaaacca gcacctggag gccgtcggcc ggcttgaaac
     4921 ggaggcgggc ggccgaaccc cggaagagga ctacccgcag ctggctcccg gcatccttga
     4981 ggctgaaata gaggtggccg gagatgggct ggcgcaggga gctcacctcg ccctccaccc
     5041 agaccagcgg gaaggccgat tccaggcggt gccggacggc ctggctcaac tcggtgaccg
     5101 tgtagatccg ctcggtggga gagccggatc cctcggtttt ttcctgaaaa agatcctcgc
     5161 ccataacgga agcctcaggt aagccggccg atcgggtcaa cgtgaacggt gacccggttg
     5221 acggccggca cctcttcctt caagcgatgt tcgacccggt cggcgatctc gtggccccgc
     5281 tgaacgctga ggttgccgtc gaccaggatg tgaagatcga cgataatgga ggggcccagc
     5341 atccgggtgc ccaggtcatg gatgtggcgc acgccctcca ccgaaccggc cacggcgcgg
     5401 atgcgggcca gggtcggccc gtccacggaa ctctcgacca gttcggagaa gacgtcctgg
     5461 ccgatcacca ggccgacgtg aatgacgaag acggccacca ggatcgccgc cagcggatcc
     5521 agaaagggca ggccgagccg ggcgcccagg atgccgagaa aggccgggat cgaggagaag
     5581 gcgtcggacc ggtggtgcca ggcgtcggcc cgcagggcgg tgctgttgat gcggcgggcc
     5641 acccggatgg tgtactggta gaggaactcc ttgagtccga tcgaaaagac ggcggccagc
     5701 agcggcagga aggtcggaac gtgaaaatgg cgggtcagca gtaccaggac cgcctcgtaa
     5761 ataaagagaa aaccggccgc caccaggaag agggagcagg cgccggcggc catggtctcg
     5821 aatttgccgt gcccgtaagg gtggtcaggg tcggagggac gccctcccag catgagcccg
     5881 aagaagacaa tcgaggtggc caggatgtcg gaaagggtgt ggaaggcatc ggtcaccatg
     5941 gcctgcgaca tcccggccag accggcgtaa aacttgaagg cgaagagggc caggttgatc
     6001 gccagcgccc agatgatgtt gcgccgtccg attgctaatc tttccatcgg tcagggccgg
     6061 tcaatgaccg gcgagtagcg gaaaggcctg ggcggccggg tgcctgaagc ccggccgggt
     6121 aacaggagta acggccgtcc cggcttcagg cggaccggcg tttcgactcg atccaggcgg
     6181 tggcgttctc gatccggacc gaggtaaccg ggtgggtgct cagcggcacg atgatcaacc
     6241 ggggctgctt cttttcgatc tcggccagtt tcttcaaaaa ggagagcatc ccctccggtt
     6301 tgtagccgct cttgagcccg tattgcatgc ccagctcgtc ggcctgcagt tcatccttgc
     6361 ggctgtagga gagctggcga aggttgaagg ccaggttgac cgccttctcg atctcggcgt
     6421 aacgctcctc ggagaagatg atcgaggcgg aaagggagta gaaaacctgg ctctggagct
     6481 gcttgaccgc gtggcgcgcg gcgacgtggc cgatctcgtg gccgaggacg caggccagtt
     6541 catcatcgga gcccaccagt ttcaaaagcc cggtggtcac gtaaacatag ccgcccggac
     6601 cggcgaaagc gttaacctgg tcggtctcca ggacctggaa ggtgaacggg atatccgacc
     6661 ggccggcgtt gacagccacc cggtcgccga tcgtcttgat ccgcgcaagc agcgcctggt
     6721 cctggctggc cttgtactcc ttcttgatct gggcatcgac ggctttcccc atggaaagtt
     6781 cgtcctggct cgagtagagg gtgctctccg acttgccggt caccgggttg tagacggtgg
     6841 tgcacccgga cagccagggc accatggtga acagaaccag gagcagcccc agccagttga
     6901 tcctgcctcg gttattcatg ggcgcgccct cctcgttgct gaagatgcac ggcaaacgat
     6961 ttcagaacca tccggtcata ttttaccaca agtatcggcg ggcggaattc ctaccggggc
     7021 cggggcggag cgccggaggg cttcttcacc cgctcctcca gcaggaagta aaggaccgga
     7081 atgaagaaga gggtcatcag cattgccacc agcaggccgc cgatgatcac gatcgccagc
     7141 ggccggaaca tctcggaacc ctcgccccgg gcgatgacca ggggcaccat gcccatgatg
     7201 gtagtcagcg aggtcatcag gatcggccgc agccgcagct ggccggcctg gagtagggcc
     7261 tcccggagcg gcaggccctt tttgcgcagt tcgatggcga aggtcaccag gatgatgccg
     7321 gtctcctcca cgacccccac cagcataatc aggcccagga aggaggtcag cgaaagggtg
     7381 ttgccagtca ggaagagcga ccagatgacg ccgatcaggc tgaagggcac ggcgaacatg
     7441 atcacgaaag gatcgaggaa ggactcgaac tggctggcca tcaccatgta aaccaggact
     7501 acggagagaa tcagggcgaa gagcatgacc cggaaggact tgacctgttc ctcccggccg
     7561 ccggtgaagg ccagcgagaa gcccgaaggc agcgagagcc gccgcagctg aggttcggcc
     7621 tcattcacga cgctgctcag cgaccggttg taaatctcgc cgctcacctc gacgtagcgc
     7681 tcctggtccc ggcgcgagat cgagaccgga ccggtcccgg cccggatgtc ggccacctgc
     7741 gaaaggcgga ccagcccgcc gcccggattg gccaggtaga ccccggccag gacctccgga
     7801 tcggcgcggt cggagtcccg gagccggatc atgatgtcgt actgttcccc gctctcccgg
     7861 tactggttgc cggtatctcc ggcatagtag ctcttaacct ggctggaaat gtccgaaacg
     7921 ctcaggccca tcaggctgga cttgtcacgc cggaccttga tctggtactc gggctgcccc
     7981 tcgtcccggc tgagcgagac gtcggagacc ccggggatgc cgcccaggac ccggctcacc
     8041 tcggccgcca gctggcgcga tttctccagg tcgtaaccct tgatttcgac cgtgagcggc
     8101 ttggcggagc cgaagagcat gctggccatc ggatcggagg cgtcgaagag gacccgggcc
     8161 ggcggcgcct tgagcagcgg tttcaagctc tcggcgatct ggcggccgct gcgctgtcgt
     8221 ttttccttgg agatcagctt gatgataagc tcgccccggt acccggccgg cggcttgccg
     8281 ccgctcatgg cgaccatggc gctgttgccg gacatgcgcc cccagtggcc catgatcacc
     8341 tcggcttccg ggacgttccg ggccaggata tcggtcagct tcctgatcat ctccccggta
     8401 acctcctggc gggtgccgac cggcatctcg accgagaagg agatcatgcc ctggtccatc
     8461 tcgggcatga actcggtgcc gaccagcgga accagcagca gggagccgag cagcatcagg
     8521 cagctgaaac cgaggctgat cttcttgtgg ttgagcgtcc agtccagcag gttccggtag
     8581 ccggccgaga ggttgcggaa gaaacgttcg ccgctctcca ggaccgcggc ctggattccc
     8641 cggcccgagc cgccggtccg cagcaggcgg ctggaaagca gcggtatcag catcaccgcg
     8701 gtcaggagcg aacagagcag gcagaaggag aaggagaggg ccagctgctt gaagagaatg
     8761 ccgctgacgc cgccggtaaa gatgatgggc acgaagacgg tcacgaccgc caggaccgag
     8821 gcgaccacgg cgccgaagac ctcctgggcc ccgttaaggg cgctttcaat ggggccctcc
     8881 cccgcctcgc ggtggcgcag gatgttctcg aagacgacaa tggcggcgtc gaccaccatc
     8941 ccgaccgcga tccccaggct ggaaagggag atcatgttca gggagaagcc ggcgccgtac
     9001 atcatgaaga aggtggtgat caggctcacc ggaatactga tggcaacgat gaagctgctg
     9061 cgcaggtcgc gcacgaagaa gaagatgacc aggatcacca gcaggccgcc ctggagcagg
     9121 gaatcgcgca ggttgttgat ggaacggcgg atgaagtcgg cctggtcgaa gagggtgcgg
     9181 aagtggacgt cgggcggcag ctccttctgg atgcgggcca tttcaagctt gacccggttg
     9241 gccacctcga ccgtgttgac cccggactgc ttctggacga tcatgaagag gcccggccgg
     9301 tcgctcaccg tcaccccgcc gaccttttcg gcgtatccgt cgctgacggt ggcgagatcg
     9361 gacaggtaga ccggccgccc ggcccgctgg gtgacgacga cccggttcag ctgcgggatg
     9421 gtcagctcct cgggcgtgcg caggatgaaa tcgtaggcgc cgctcttgag aaaaccgccc
     9481 ggctcgttga ggttggccga ccggatggcc gcggaaacct gccggagcga aacctggtag
     9541 gcctccaggc gccggcggtc gaccgccacc tggatctcgc gccggttgcc gccgtaaatg
     9601 gaaacggaag ccacccccgg cagctgctcc agctggcggc tgacgttctt gtccaggtac
     9661 tcgtaaatcc agctgtaact gctgccggcc tcgacgccga ccatcaccac cggcatcatc
     9721 gaaaagtcga acttgatcac gaaggggcgc tcggccgcgt cgggaagcat cggcattacc
     9781 tggccgatct tgtcccggat ctcgttggcc ttgtcctcga ggttgacgcc ccattcgaag
     9841 cggagctgga ccagcgaaat gtcctggctg gaggaggact cgatcttgtc caggccgctc
     9901 acggtggcca tcaccttttc gatcggacgg gtgatctggg tctccacctc ctggggtccg
     9961 gcgccctggt aggtggtgac caccgccacc gaggggattt cgatgtccgg cagcaggtcc
    10021 atgcccaggc gccccaggga gaccaggccc aggatgatca gggccaggaa gagcatggac
    10081 gtcaggaccg gccgcttgac gccgagttcc ggaaggttca ttccgatatc tccactttgg
    10141 ccccgtcgta gagagagccg aaaccgctga tcaccacctt ctcaccctcg gccaggccgc
    10201 cggtcacctc gaagaggggc ccggactgga agccgagctt gacctggcgc aggctggcgg
    10261 tcccgtcctt aacgacatac acggtgggat tgtcaccctg gaggctgagc gcctcctggg
    10321 aaatggtcaa cacgccctgg tgggaaccga ccagcacctc ggcccggacg aacatgccgg
    10381 ccttgaaacg gccgccgggg ttggcgatcg cgatctcgca gccggcggtc cgggtgaccg
    10441 ggtccagcac cgggctgagc cgggtgacgc ggccggagac cgaaaggtcc ggaaaggcgt
    10501 cggcgacgat cttgaccggc tggccgagcc ggagccgggc caggtccgtc tcgcccaccg
    10561 gaatcttgac cttcagttcg gcaaggtcga ccagctccag gatcgggccg ctgcccgcgc
    10621 ctatgaagac ccccggatcg aagaaacgcc ggctcacgaa gccgccgaag ggagcggtta
    10681 tcaggcagtc ggccagctgc tcctggacca tcttgatctg gacctcgaac cgctcgaccg
    10741 cggccgcggc cgcgtcgcgg cgggcccggg cattatccat ctcctgggcg gtaatcacct
    10801 cttccctgaa gaggctttcc atgcggccga agttttttcc ggcattctcg agttcggccg
    10861 cggccgccag gcgggccgcc cgggcctcca gcaggcgaag ctggtaatcg cgggtctcga
    10921 tccgggccac gatctgcccc ggcttcaccc agtcgccctc ctggaagaga acctcctcaa
    10981 gcttgccgct cacccgggcc aggatcggca cggcccgcga ggcctccagg gtgccggaga
    11041 aggataattc ctcggtgagg tttcgcctcg ccaccggctg gagagtcacg ttcagggtct
    11101 cggcttcgcg ggccggcggc ttgacgctca atcgctgctg gacccgccag ccgaccaggc
    11161 cgacgatcac tgccagaaaa aaaaggagaa gctttcggcg tttcatcttg tctcccggaa
    11221 atttgacggg gcggaccggc cggtcattcc cgccccaggc tgcgcctgag ataggcctcg
    11281 gcctggagga aatcgtaaat ggcgttgtag taattggtcc gggcctgggc cagggcgaat
    11341 tcggcatcca ggacctcggt gttggtggcc aggccttcct ggaaacggat gccctgcatc
    11401 cgcaggactt cctcggcctg gccgaccgcc tggccggccg tgtcgatccg gtccagcgac
    11461 gactccagcg agagccaggc gttcttcact tccagccgga ccatccggac ctggtcatcc
    11521 agggccagcc caacccggga ggtctcgctc cggctggcgg ccagacggtt cctgacctgg
    11581 ccgaaattcc agacattcca ggagaagacc agcataagct ggctgctgtc ctgccaggag
    11641 ccgcggaagg cgtcggcctg ggattccccc tggctcaggg tgagcgccag ttccgggtgg
    11701 taaccgctgc gggctaaaat ctcgttctgc tggctccggg ccagctgaag ttctccgtag
    11761 cgtatctcgg gccgctgctt cagggcctcc tcgtaaagga cctccggggt aatcccggac
    11821 cagttgggac gggcggtggt atccacctcg gccaggtcca gcgccccttc cagcggccgc
    11881 tcgagcagcc ggttcaggga gctgtaggcc agggccagat tattgcggac cgaggtgagt
    11941 gagtcccggc tgtgggccag ttccacttcg gccttcaatt tctccacctt gggcaggacg
    12001 ccgatctgga cctttttctc cacctcggcc aggtggcgct gccggttggc gaccagttca
    12061 tcggccacgg agacggtctt cttcaagcgc aggatggtgt aataggagtt ccggacctgg
    12121 agcgccaggt ccagcccggc ctggtcataa acatgccggg ccaggccgac gttcagaaga
    12181 gccgactggt aggcggcctt gagccggccg ccgttataga tcggctgggt ggcgctgaag
    12241 gcgagggagg cggaatcctg cccgttgctg ctgccggcgc tggacaggcc ttcccagtag
    12301 ccgtaattgc cggagaggtt gaaggacgga taaagatagg tcttctgttc ggccgccgcg
    12361 taggaagcgg aggcaaggcc ggcctgcgcc tgctggagca gcaggctttt gctccgggcc
    12421 tcggccaccg ccttctccag agtcagggtc tcggccccgg ccgcggcgcc atggaacagc
    12481 ggggaaatta tcaaaccgaa caaaacgaag accagcctgc tgatgcgcat cttcggactc
    12541 atcgaatctc tcctgaatgt gaaagtaacc tccgaccacg gggccgcagg atattgacaa
    12601 agttttggcg gatattatat catcatcgac acgggctgtc aactgaccgg ccagtcagtt
    12661 taaaaagctt ttttctaatg atgacaagct tttaaatgat caaaaaaaaa tctaattcaa
    12721 gaaaatcggg acccccgtcg gagaagagcg gcgccatcct gaaggcggcc aagcgcctct
    12781 tcctcggccg ccggttcgac cgggtgctga tggacgacat cgcccgggcc gccggctgcg
    12841 ccaaagggac cctctacctt tacttccgga ccaaggagga cctctacctg ggcattctaa
    12901 cccgcgattt cgatctcttc catcgccggc tcgaatcctc gctccggaac cccgggaccg
    12961 cctgggaagc gctcggcaag ataaccgccc tcctcttccg cttcctcaag ggcaactacg
    13021 acttcttcgt tctctcccgt caggcggaaa tccatctccg ccggaagcat cgggagctcc
    13081 tccgcgaaca gcacaaccgg atcatccaga tgctggccgg actggttggg aagggaatgg
    13141 aggagggagt gattcaaaac ggcgatccgg aaggctatgc ccatatcctg ctcgggatga
    13201 tcttcgcctc ggcccacccg gccagccggc cggccgccca gacccccaag ctggtgctcc
    13261 ggatctttgc cgaagggatc cggagccggc cggaccgcgg tcctactcga acttcctccc
    13321 ggaccggatc gcctcgacgg cccggttgaa ggcccgggcc aggtcgtaaa agtaatcctt
    13381 ctcccgaaag agcagcgggt atggaggcag cttgccggcc gccagctggt tcatcacctc
    13441 ttccagccgg aaaagcgggc cgcagatgcg gtgcatgatc cgcagctgga gcaggacggc
    13501 cagaaagagc agcaccgcgc cgccgatcag aaattccagt accagcagcc gcagcacctg
    13561 cccctggagg tgctccaggg ccgccgggtc ggggatgggt atccggagca ggaggttcca
    13621 gaccgtgagg taggtgacca ggccgcccag cagcagggcg aggatgatca gccagaccac
    13681 caccagcgaa taccggatct ggaccggctt gttgatgatg cgccagaagg aacgcttgaa
    13741 cttcggccgg gccggccggc tcccattctt tccgtccatg ctctccatcc ccctcccctt
    13801 gccggaatcc cgggcgcgtg gaagcttttt acttgaaact gccggtcgcc tgcccctcga
    13861 tccagaccag gtcctcttcg aattcggcgc cgccgggctg cccgtcggca ccgcggctgc
    13921 ccaggatgta ggtggtcgcg cgagaaaagg ggctggttat cgacaccagg aagtaattat
    13981 gcgggcggac attgctgggt atccagacat aaagggtgtt gaccttgtcc agggcaacgg
    14041 tcttctccat gtggtcaaga ttggggttga attcgttcgg atgaaagatc tcaactccgt
    14101 tgaggatgat gcggccggag gccacctgga agttgtggat aatcatcccc ccctgaccgg
    14161 tgggcggcgt ccggaaggtg aaggtttctt cataaggctg gccgcccggt tcccgccggc
    14221 actgctgggg accgaagacc acgccgttgg tcacgcagta aaagtagggg gtcttccagg
    14281 ggtcgatggg aatagtctta aggtagggac cctgccagcc gcggcgcccc gggaagggag
    14341 agtcgcggta ggtcagcgac tccagcgtct cgttcactga gggcgggtag taaccgaggt
    14401 cgttgcggat gccgtccacc agggaggcgt actgcgaaat ataggccttg gccacggcga
    14461 tcttcgtctt ctgagaggcg gcgcgaatca aggaaaaacc tatggctgaa agcaggaata
    14521 ggaccagcag gccaaccacc agctcgatga tggtaaagct gctctcgcgc cgggtcctgg
    14581 catccatctc gacctccctc gcgtttcgtt actcgaattt cttgccggac cggatggcct
    14641 cgacggcccg gttaaaggat tcggccaggt tgtaaaagaa gtctttctcc cggaagacca
    14701 gcggatgctt gggcagtttt ccctccgcca gctggcccat gagctgctcc agccggaaga
    14761 gcggaccgca gatgcgatgc aagatgcgga gttccaggac gccggccagc accaccagca
    14821 ccacgccgcc gatcaggaac tccagcgtga gcaggcgcaa cacctgcctc tggagctgct
    14881 ccagagccgc cggatcgtga atcgggatct ccagcaggag attccaaacg gtcaggtagg
    14941 taaccatccc gcccagcatc agggcgatga tgatcagcca gatgaccacc agcgagtaac
    15001 gaatctggac cgtcttgttg atgatgcgcc agaaggaacg cttgaacttc ggccggctct
    15061 taacctcgtt cttctgatcc attccccctc ctcgatgaaa cccttgaaat gcttatcgga
    15121 agctgccggt cgcctggccc ggaagccaga ccaggtcggc ggcgaaacca tcgccgccgg
    15181 gctgcttgtc agccccccgg ctgcccagga tatagcttgc cttgcgggaa agcgggctgg
    15241 taaccgatat caggaagtag tttgaatgct ggacgttgct gggaatccag acccgcagcg
    15301 aattccgggc caggagagtg acggccgcct ccagatggct gacctgggga ttgaactggt
    15361 tcggatggaa gatttccatc ccgttaagcc agacccgggc cgaggccacc aggaaattct
    15421 cgatgatcag gccggcgttg ccggtggcgg tggaatcaaa gacaaaggtt tcgtcgtaag
    15481 gccccccgcg ctgctggcgc cggcactcct ggggaccgaa aaccaggccg ttctggataa
    15541 ggtagaagta atcgttccgc caggggtcgc gcggaagcgt cttgaagtag ggaccctgcc
    15601 agccgcggaa tttgggggag ggcggcccgg ctgaggtcag cgactccaga ttcccgtttt
    15661 cggagggcgg atagaagccg caatcattac ggatgccgtc gatgaccatc gcaaactgag
    15721 cgatgtaggt cttggccacc gcaaccttgg tcttctgaga ggcacttctg ataagagaga
    15781 agcccaggcc ggccaggagg aaaaggataa ccagccccac cgccagttcg atcagagtaa
    15841 aaccgttgtt tttccgtttc aggccattgg gcatattgtt caattatacc aagtcagggc
    15901 tccgactgca acagttcctc gcccacctgg tgcaccggcc cggcccccag cgtcagcaat
    15961 acatccccgg ccaccaggtt ttcccggagg taatcgacca cccgggcgaa agaatcgaag
    16021 aacccggcct tcttcccgtt ggcccgcagg cgctcaacca gatcggcggc cgaaacactg
    16081 cgccggctct cctcgctgtc ccggacgaaa taaatctcgg gcacgataac ctcgtcggcc
    16141 agccgaaagg aacgggcgaa gtcctcgaga aagaaacggg tccggctgta ctggtggggt
    16201 tggaagacca ccagcaggcg gccggccggg taaaattgcc ttatggcggc cagggtggcc
    16261 acgatctcgg tcggatggtg gccgtaatcg tccagcacca cggccccccg gtagatcccc
    16321 ttcttttcaa ggcgccggtt gaccccccgg aaggccgcga ggccgcggat gatcatctcc
    16381 ggttccaggc ccagcaagcg gcaggtggcg aaggcgccca ggctgttgag aatgttatgg
    16441 atgcccggca ccgcgatggt caccgttccc aggcactcct cccggtaaaa ggcccggtag
    16501 gccgacgagt ccggacgcag ttccatcgac tcggcccgga aatcggcgcc gctgaaaccg
    16561 taggtgtaga cgggccggtc aagggaactg acgacctcct gaacattggc gctctcccgg
    16621 cagacgaccg cgaacccgtc cgtcggcagc cgggagagga attgcaggaa ggcctcctcg
    16681 atctcctcga tgtccttgta gtaatcgagg tgatcttcct cgatgttggt caccagggcc
    16741 acccggggcc ggagcgaaag gaagtttctt cggaactcgc aggcctcggc caccaggtac
    16801 cggccctggc cggtcccgga gttgccgccc agcaggggta tttcgccgcc gatgacaaaa
    16861 ctcggatcga gaccggcggt cttgaggatc acggcaacca tggcgctggt ggaggtcttg
    16921 ccgtgcgtgc cggccacggc gatcccgaaa cggcgccgca tcagagaacc gagcgcctgg
    16981 ctgtaggtaa tgatcggaat ggagagggcc tgggcggcga gtatttcggg gttgtcatgg
    17041 ggaatcgcgg ccgaaatgat caccacctgg gcatcctccg gcaggttgtc ggcccggtgg
    17101 ccgatcagga tgcgggcccc gtcccggcgc atctgttcca gcaggagatt gtcgcccagg
    17161 tcggaaccgg tcacccgctt gccttcgccc aggagtatcc gggccacgcc gctggtgccg
    17221 atgccgccga tacctatgaa gtggacccgt tccagccttt cgagatcaac aagtatttcg
    17281 cccattcccg ctccgtttcc gacccggctg cttccgggtg aagtccctgt ttcaagataa
    17341 ggcggctata aattatatta tatcttgcgg ctggagatat attacaggag ggtggcgccg
    17401 tcgggcaaat ctttcagacg ccgtccggca gcatggccgc tccgaaagcg accacgaact
    17461 ggggcggatc caggacctgg accggccggc cggccaggcg ttccagggcc agccgcaggg
    17521 ccggactgcg agcgccgccg ccgcagaaga aaaccggctg ctcctggaac tggcgcagca
    17581 tggcggctac ccggctggcg acggcctcaa agaggccggc ggcgatgttc gtcttcgact
    17641 cgccgtgggc gatcagggag accacctcgc tctcggcgaa gacgctgcag gtggcattca
    17701 gccggcgggg ggcttcggcc gaccgggcca gctgggagaa gccttccatg tcggtctcca
    17761 gcacctgggc catcaactcc aggaaacggc cggtcccggc cgcgcacttg tcattcatga
    17821 gaaaatcgct gaccgaccct tcgccgttga tctcaatcac cttggtatcc tgcccgccga
    17881 tgtccaccac caggcccggc cgccggccgt tcatccggaa ggcgccggcg ccggccgccg
    17941 agatctcggt aatcacccgg tcggccaggc cgcactgg
//
LOCUS       DAOQYN010000013        14944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_4463_length_14944_cov_5.145947, whole genome shotgun sequence.
ACCESSION   DAOQYN010000013 DAOQYN010000000
VERSION     DAOQYN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14944
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4463_length_14944_cov_5.145947"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..460)
                     /locus_tag="PKN80_01185"
     CDS             complement(134..460)
                     /locus_tag="PKN80_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34661.1"
                     /translation="MKKRPSLKGKGAEIFLSGEPAAGRATRPTAAGHETKREAGEEKE
                     KEKATFYFPPKLLSDLDEAWMKLRRENRRIKKSDLVRAALVEALADFKRRGRESLLAA
                     AFQKRA"
     gene            complement(438..1220)
                     /locus_tag="PKN80_01190"
     CDS             complement(438..1220)
                     /locus_tag="PKN80_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNQ34662.1"
                     /translation="MGAVIYSVSNQKGGVGKTTTSLCLGAALAEQGRRVLLVDFDPQA
                     GLTTSLGFNPDEFERTVYDLLLNGDTLKIDQVIQAVRTTGLDLVPANLDLAGAEGELI
                     GEIGWDRTLKELLVPIQDRYDFILIDCPPSLGVLTTNALIAADRVIIPVQSEYLALKG
                     LKQLNDIIAKVKRKANNRLRARILRTMHDTRTLHSREVVEELAKVFPEEVYRTVIKRT
                     VRFADAALAGQSILSYAGRSEGAQAYRDLALEVLKDEETTKP"
     gene            1429..2001
                     /gene="amrA"
                     /locus_tag="PKN80_01195"
     CDS             1429..2001
                     /gene="amrA"
                     /locus_tag="PKN80_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNQ34663.1"
                     /translation="MLNQEQRRFLLQAARRSILNYLENGLTGVAETADPVLLEKRGAF
                     VSLRWDKDLRGCIGLIEGKYPLNETVCRMAIEAAVGDPRFPGLTIRELKEVIIEISVL
                     TTPERVRGPQDIVMLRDGVIVRQGYCQGVFLPQVAEEQGWDKEQFLSFLCEYKAGLPR
                     EAWKEKETELYTFQAEVFSEVDFPPSSPSK"
     gene            complement(1947..3053)
                     /gene="murG"
                     /locus_tag="PKN80_01200"
     CDS             complement(1947..3053)
                     /gene="murG"
                     /locus_tag="PKN80_01200"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNQ34664.1"
                     /translation="MRLVIAAGGTGGHLFPALALALALKEAGARPLAAGHITPQLRRE
                     FEAAGIPWVDLPAVRLPRRKAGYLWLPFRLIWAWLAAAFLLARRRPAAVIGMGGYASA
                     APAAAARCLGIRLYFCEQNLLPGKVTRFFSRWSEAVFTAFPESSGYLQSKRVLNFGNP
                     LRPSLKPVDRKAARRELGLDEGRFTLLVLGGSQGAHRINLKVLEALPGLNREQVQFIH
                     LTGPADHPAVEAAYRTGGWSAFTAPFQSAMEVPYSAADLVLCRAGATTIAELIRFGKP
                     ALLIPLPSAAEDHQRLNARWLAERKAARVIEEGDLTGKNLAEEVSGLAGAPDRLAAMA
                     AAAGGLARPDPARSIGAYLLGELGGKSTSEKTSA"
     gene            complement(3050..4177)
                     /gene="ftsW"
                     /locus_tag="PKN80_01205"
     CDS             complement(3050..4177)
                     /gene="ftsW"
                     /locus_tag="PKN80_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNQ34665.1"
                     /translation="MLNGEIRLKKPWPVYRKLSFLVVLLAALGVLLVYDITFLQAGQK
                     YNDQYFFLKRQLLWVMLGFACFLGALRINLDRWRRYARISLLVAIALLFLVLVIGREI
                     NGAKRWFSLGFFNFQPSEFAKLAVIFYLADFLARKERVITNPVRIFMPTGLLVGLAGA
                     LILKEPDYGTAALVLALLFILYFIAGAPRRLLFLGILVLAVLGGLFVIQSDYHRKKMQ
                     TWLNPGADPLGAGYQINSSLLAVGSGGIFGRGLGQGQQKYDYLPEPHTDFIFANIAEE
                     LGLIGSLLFLSPFFLLIHLGLETARRSADLFRFYLGTGLTSLLALQTLIHLGVVLSLL
                     PNKGLTLPFISYGGSSLLFTFLGTGLLVNLARDLPPDEPVQ"
     gene            complement(4128..5474)
                     /gene="murD"
                     /locus_tag="PKN80_01210"
     CDS             complement(4128..5474)
                     /gene="murD"
                     /locus_tag="PKN80_01210"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNQ34666.1"
                     /translation="MELAGKKILVIGLGESGLACGLFLAGRGAEVRLTENRRGPGLET
                     ERGKLEAAGVRVELGGHSEAFLEGLDLVVTSPGVPPESRPIQAALQSGIPVWSELELA
                     YRFCPAPILAVTGTNGKSTVVTLLRDVLKAAGRPVVLSGNIRPAFISVVDRLTATDTV
                     ILEVSSFQLEWIERFRPKLSLLLNITADHLDRYPDFGSYRKAKLRIFENQAGDDIALL
                     NYDDPESRVAGAGFKGRTFFYSRREKEGRFFSDGRHLFLPDGEKFLEIPPSRLWGVHN
                     QENILAVCAAARLYGLPSEAMAGAIRNFQPLAHRLEPVARKQGVQYIDDSKATNVDST
                     LRALQSFPKDSGRVLLILGGKDKGGSYRPLREALSGQPAEVILIGEAAERISAELAGT
                     VPIHRTADLAGAVRLAAGRARPGDTVLLSPACSSFDMFKDYAQRGNSFKEAVAGLP"
     gene            complement(6156..7151)
                     /locus_tag="PKN80_01215"
     CDS             complement(6156..7151)
                     /locus_tag="PKN80_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HNQ34667.1"
                     /translation="MKKPTVVSLIKKELQTQLFSPADRERLNRLARIDEIEPDPEKPP
                     RLPAETVACLTSWGSPRFTEEILTAAPDLKLVVYAAGSVRPVVSEALWKRGIVVTSAS
                     AAIAIGVAEHTLGLMLSTLKRVCRFNELLHQGGWYHQEERVKIREIYGLTVGVVGAGH
                     AGRHFIRLLKNFEVRILLYDPYLDPAEAVRLGVEPAASLEALIPQVDVLSLHAPALPS
                     TRDLINRENLKLFKDGALLINTARGALIDETALYEELLTGRLTACLDVRYPEPPVPEP
                     PLWKLPNVILTPHIAGGVTDNMKRLGKYALRELENFFSGRPPVYPVTERDLERMA"
     gene            complement(7192..7854)
                     /locus_tag="PKN80_01220"
     CDS             complement(7192..7854)
                     /locus_tag="PKN80_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNQ34668.1"
                     /translation="MKKLLLLGLLLLGGGPAAAEDFVHCWGSCTTSFQTGLDAREHNI
                     LLAVEKLDGCRLGPETEFSFLETVTRQLKPGALGYASSLVEGRRRPAPGGGLCQVSST
                     LYCAALAAGLQIQERHPHSSLVGYLPPGLDAAVSTEAWTDLRFRNRQAATLQIRARVQ
                     AAALSIGFYGTETPEWEIRLKASPPEKRAGWFYTVTRRDYFRAGREAFSEIISRDRYP
                     AP"
     gene            complement(7851..13034)
                     /locus_tag="PKN80_01225"
     CDS             complement(7851..13034)
                     /locus_tag="PKN80_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNQ34669.1"
                     /translation="MKTSRKVALFLLLAGSLWLAENLRADTISFQVDRSDGQPVAGAR
                     LTVTRLHAKETAAATTDRTGRAEAAVPGPGYYRIRAEAAGYLAENLRSLEINQAKYGE
                     VIQRLTLRRPGSISGFVYDKSGRPLAGGRVNNTPCDGRGFYLLKNLSPGPAYLNAQAP
                     GFVRQYRESVPVKEGEETGGVNFNLEPGGRLSGRVIAAETGRPIARAWVNTEGPTYLS
                     AQTDSDGNLLLDGLAAGTYKISVYTQGYREGSAANLEVRPGQTTRLPGEIRLQLRPKS
                     FSLNRRNYIFQPGESVQIRYNAFRVTKLRLELYPFNPRAEAEKILGQQLNPHTLEWMI
                     RQADLSGRPPAASLLFTVRYPQPLSDLYDRSVTLKSLPAGLYLVRLTSPEEVPDRPGG
                     EDRFYCLVTDLALVRKSAPEQNLLFACGLADGRPRPETLIWTLGGPGAPRLLGRTDAS
                     GRLETNGGGLALAEAGTSLALLNLPTARPSNVRSDPTIAYLYTERPVYRPGQTVYFKG
                     FLRLDHGNRYEVPPARKARVQVRDPEDRLVSDTWLPVSENGSLAGSLELAAEPRLGNY
                     TLSIHPDQAIAASGTSGFQVLEYRKPEYQVEVEADRDRYLPGEKIRFRLRASYYFGQP
                     VANARVNYLVYERSGWWADYDYEEYENENGGPGWGYGKLLRQGEAQTGPDGTCLVELP
                     GRGAYENETRFTLEARVVDRSQREVIGRASADAVPGAFRIGLVTPRYVYYPGEAVPLE
                     ITVRTWDDRPVAGLPIKVFVALERYEKNRYTWTELQAETAVTGPEGRTSLNLKPGRSG
                     YLKVYAQASDEYKNLVNGLRYIWVAGREYTSAWSSSRELEIVTDQKSYRPGDTARVLI
                     TTAFPGQPILLTVEGTRLHESRILQPEAQTSLLEFPVTAEHIPNFTLGLTAVRNRELH
                     QAERTLPVVEPNRFLTVEITPDRKEYRPGEISRFAIRTLDSAGAGTPAEVSLGLVDKA
                     IYAIAPETTLDPDRFFYGRKPNRVSTGYSFYTWEYGGAKDLAETGLRRNFKDTAFWIP
                     ALWTDREGRASLEVALPDNLTTWRATARAVTPETRVGGARREILVSKPLLARLAAPRF
                     LVEGDRFTLSAVIHNYTGADQPVTVILEARGLRLLETAPQEATIPAGGSIRCQWLAVA
                     GEAPEAELALRAGNRDYSDGLETKIPVLPYATAVLEVRSGECRESALNSFKLPAAAAS
                     PKLTSYLYPSLASGLFRSLDYLAGYPYGCVEQTLNRFLPTLQVARAVRELGVSDLRLL
                     SDDPKAFQKMLEELPAMVNRGLARLYDYQHADGGWGWWAADASHPYTSALVLYGLTLT
                     EECGYPVDPEVRRRAVSFLAEQVKSLEDPDARAYAAFSLSQAEPAVPSGLKGTLAKLF
                     QERSRLNPYGRALLALSFHKADPAGRVAAQVAEELLADLKPLTPDISFWKADRPDRYS
                     WVDSDVEASAWALKAVLALAPQSPEIPKIIRYLVASRRLGRWRSTKDTAVCVSALTDF
                     LRRGTELNPDFTAELTVNNQPLAREKFDRDSLREFVRIITAPPGTLKAGEENTLSLKK
                     TGAGNLYYSHALDYLDRSRLQEPEDRGFTVKREYRRLQAGAGDAAGNPEEKPFAAGDT
                     LKVGERVRVTLTITGRDFYEFAVIEDALPSGFEVVETPENEAGGNWYCRREVHDERIV
                     FFTPFWGLDAEGKGTRTLVYHLRPETTGDFQVRPARASLMYFPEVNGRSDGFHLRVRE
                     E"
     gene            complement(13031..13630)
                     /locus_tag="PKN80_01230"
     CDS             complement(13031..13630)
                     /locus_tag="PKN80_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 family protein"
                     /protein_id="HNQ34670.1"
                     /translation="MIPQKAMRIGWLSLFLLIVAAGPAFPDPIPGKMALAEARQALNH
                     GWSTPTSNPLPGKPPAELNRPGAVFVTLLRNGRVRGCAGSFESLFPDLRQEIAHFTRV
                     AAWQDPRYRPVGPEEAAGITIILTFPQDLEAIDSLDRYRPDRDGLVVRQSGRSGVVLP
                     GEARTVEYAFRKCLRNAGLPPEARPQLYRFQAIQYRETK"
     gene            complement(13627..14763)
                     /locus_tag="PKN80_01235"
     CDS             complement(13627..14763)
                     /locus_tag="PKN80_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ34671.1"
                     /translation="MTRSKKPASSRGPQSAPARIYFADIAYESAEPDRTLPAKFKRML
                     EKLALEKRVKEKSVTIKMHLGGHLGYTTIHPLFVRLLVEALKTAGAKDLKVMDNSAKT
                     AFMRGYTHEVLGCPVIPCFGQSGRYYYREPIGFRGLDEALLSGEALDSDFFIDLAHLK
                     GHGDCGFGGAIKNIAMGIVPSETRRKIHGLEGGLVYDADKCIFCLKCHRACPNGAITI
                     NREKKKIGFFFHNCTYCQHCVLACPKKAIRMENRRFEDFSRGLALVTSAFLKKFAPEN
                     LLFINFLMNITIFCDCWGMSTPSLVPDIGILAAEDITAIETASLDLIKTENLLPNGLP
                     KDRKLSKGKGHLFERIHGKDPYLMVRYLEEIYGGTRVYKRIEVK"
BASE COUNT         2512 a         4542 c         5168 g         2722 t
ORIGIN      
        1 ggcctgctct ggacccgcct gggcgtgcgg acgccctttt atttcggcag cctgatggct
       61 ctcctggcgg tgctggtttt tgtgctcccg ggccggcgga cgccggcggc cgttgcccgt
      121 tccggatgag agatcaggcc cgcttctgaa aggcggcggc caggaggctt tcccggcccc
      181 ggcgcttgaa gtcggccagg gcctcgacca gggcggcccg gaccaggtcc gatttcttga
      241 tccgccgatt ctcgcgccgg agtttcatcc aggcctcgtc gaggtcggag agcagtttcg
      301 gcggaaagta aaaggtcgcc ttctccttct ccttctcctc gcccgcctcc cgcttcgttt
      361 cgtgccccgc cgctgtcggc cgggttgccc ggccggcggc cggttccccg gacaggaaga
      421 tttcggctcc cttgccctta aggcttggtc gtttcttcat ctttcagcac ctccagtgcc
      481 agatcgcggt atgcctgggc cccctccgag cgtccggcgt aggagaggat ggattgcccg
      541 gccagcgcgg cgtcggcaaa acggaccgtg cgcttgatca cggtccggta gacttcctcg
      601 ggaaagacct tggccagttc ttcgaccacc tcccgcgaat gcagggtccg cgtgtcgtgc
      661 atggtgcgca ggatgcgggc gcggagacgg ttattggctt tccgcttgac cttggcgatg
      721 atgtcgttca gttgcttcag acctttcagg gccaggtatt cgctctggac gggtatgatg
      781 acccggtcgg cggcgatcag ggcattggtg gtcaggacac ccaggctggg cgggcagtcg
      841 atcaggatga aatcgtaacg atcctggatt ggaaccagga gttccttcag ggttcggtcc
      901 cagccgattt caccgatcag ttcgccctcg gctccggcca ggtcgaggtt ggccgggacc
      961 aggtccagtc cggtcgtccg aacggcctgg ataacctggt ctattttcag cgtatcgcca
     1021 ttcaggagca ggtcgtaaac ggttcgttcg aactcatccg ggttgaaacc cagcgaggtg
     1081 gtcaacccgg cctggggatc gaagtcaacc agcagcaccc gccggccctg ttcggccagc
     1141 gccgcgccca ggcagaggga ggtggtggtt ttgcccaccc cgcctttttg gttggaaacg
     1201 ctgtagatga cggcgcccat gcttctcccg ttcgtgagtt ttttgtcctt tccgccccgc
     1261 cggcggattc ctgacaatta taacattgaa atgttatgct gtcaaaaaat cataatggta
     1321 taaatgggag catgtccggc cgggccggtt gtcgggtata atgtaaaagc cgtcggtatc
     1381 gtccggcgcc gggccggacg gcgcggtgcc ggacgaaaag gagaaaagat gcttaaccag
     1441 gaacagcggc ggtttctgct ccaggcggcc cgccggtcga tcctcaatta cctggagaac
     1501 ggattaaccg gggtggcgga gacggcggac ccggtcctgc tcgagaagcg cggtgccttc
     1561 gtttcgttgc ggtgggacaa ggatttgcgc ggctgcatag ggttgatcga gggaaagtat
     1621 ccgctcaacg agacggtctg ccggatggcc atcgaggcgg ccgtgggtga tccccgcttt
     1681 cccggcctga cgatccggga actgaaggaa gtgattatcg aaatttcggt cctgactacg
     1741 ccggaacggg tgcgcgggcc tcaggatatc gtgatgctcc gggacggggt gattgtccgc
     1801 cagggatact gccagggggt cttcctgccg caggtggccg aggaacaggg ttgggacaag
     1861 gaacagtttc tctcgtttct ctgcgaatac aaggccggtt tgcccaggga agcctggaag
     1921 gagaaggaga ccgagcttta cacctttcag gccgaggttt tctccgaggt tgattttccg
     1981 ccgagttctc ccagtaaata ggcaccgatg ctccgagccg ggtcaggccg ggccagccct
     2041 ccggccgccg cggccatggc cgccagtcgg tccggggcgc cggccagtcc ggatacctcc
     2101 tccgccaggt tttttccggt taaatccccc tcttcaatca cccgggccgc cttccgttcg
     2161 gccaaccagc gggcgttgag gcgctggtga tcttcggcgg ccgagggcag cggaatcaga
     2221 agggccggtt ttccaaagcg gatcaactcg gcaatggtgg tggccccggc ccggcagagg
     2281 accaggtcgg cggcgctgta agggacttcc atggccgatt ggaatggagc ggtgaaggcc
     2341 gaccagccgc cggtccgata ggccgcctcc accgccgggt gatcggccgg accggtcagg
     2401 tggatgaatt ggacctgctc ccggttcagc ccggggagcg cctccagcac tttgaggttg
     2461 atccggtgcg ccccctggct gccgcccagg accaggagcg tgaagcgccc ttcgtccagt
     2521 cccagctccc gccgggccgc ctttcggtca accggtttca ggctcgggcg cagggggttg
     2581 ccgaagttga gcacccgctt tgattgcagg tagccgctgc tttcggggaa ggcggtgaag
     2641 acggcctcgc tccagcggct gaagaatcgg gtcaccttgc ccggcagcag attctgttcg
     2701 cagaagtaga ggcggatacc caggcagcgg gcggccgccg ccggggcggc cgaggcgtag
     2761 ccgcccatgc cgatgacggc cgccgggcgc cggcgcgcca gcaggaaggc ggccgccagc
     2821 caggcccaga tcaggcggaa aggcagccag aggtaaccgg ctttccggcg gggcagacgg
     2881 acggccggca ggtcaaccca ggggattccg gccgcttcga attcccgccg cagctgggga
     2941 gtgatatggc cggcggccag cggccgggcc ccggcctcct tcaaagccag ggccagggcc
     3001 agggccggaa agaggtgccc gccggtaccg ccggccgcga tgaccaacct cattgaaccg
     3061 gttcgtccgg cggcagatcg cgggccaggt tgaccagcag tccggtgccg aggaaggtga
     3121 agagcaggga acttccgccg taactgatga agggcagggt cagccccttg ttgggcagca
     3181 gggagaggac tacccccagg tggatcaggg tctggagggc cagcaggctg gtcagaccgg
     3241 tcccgaggta gaaccggaaa aggtcggcgg agcgccgggc ggtctccagc ccgaggtgta
     3301 tgagcaggaa gaaggggctc aggaacagga ggctgccgat caggcccagc tcttcggcga
     3361 tgttggcgaa gatgaaatcg gtgtgcggtt cggggaggta atcgtacttc tgctgccctt
     3421 ggcccaggcc ccgcccgaag attcccccgc tgccgaccgc cagcagggaa ctgttgatct
     3481 ggtagccggc gcccagcggg tcggccccgg gattcagcca ggtctgcatt ttcttccggt
     3541 gatagtccga ctggattaca aagaggccgc ccaggacggc cagcaccagg atgcccagga
     3601 agagcagccg ccgcggggcg ccggcgatga aatagagaat gaagagaagg gccaggacca
     3661 gggcggcggt gccgtagtcg ggttccttga gtatcagggc cccggccagc ccgaccagca
     3721 gtccggtcgg catgaagatc cggacgggat tagtgatcac ccgctccttc cgggccagga
     3781 aatcggccag gtagaagatc accgccagct tggcaaattc cgaaggctga aagttgaaga
     3841 agcccaggct gaaccagcgt ttggcgccgt tgatctcgcg gccgattacc agcaccagga
     3901 agagcagggc aatggccacc agcaggctga tccgggcgta acggcgccag cggtcgaggt
     3961 tgatccgcag ggcaccgagg aagcaggcga agcccagcat gacccagagc aactgcctct
     4021 tcagaaagaa atactggtcg ttgtattttt gcccggcctg caggaaggtg atgtcgtaga
     4081 ccaacaacac cccgagcgcg gccagcagta cgaccaggaa ggaaagttta cggtaaaccg
     4141 gccacggctt ctttaaacga atttccccgt tgagcatagt ccttgaacat gtcaaaactg
     4201 gagcaggctg gagagaggag cacggtgtcg ccgggccggg cccgaccggc ggccagcctg
     4261 accgccccgg ccaggtcggc cgtccggtgg atcggaacgg taccggccag ttcggcgctg
     4321 atgcgctcgg cggcctcgcc gatcaggatc acttcggccg gctgtccgga cagtgcctcc
     4381 cggagcggcc ggtaggaacc gcccttatcc ttgccgccca gtatcaggag tacccggccg
     4441 ctgtctttcg gaaacgactg gagggcgcgc agggtggaat cgacattggt ggccttggag
     4501 tcgtctatgt actggacgcc ctgtttcctg gccaccggtt cgagccggtg ggccagcggc
     4561 tggaaattcc tgatcgcccc ggccatcgct tccgaaggca gcccgtaaag ccgggccgcg
     4621 gcacagacgg ccaggatatt ctcctggtta tgcacccccc agaggcggct gggcggaatt
     4681 tccaggaact tttctccatc gggaaggaag agatgccgac cgtcgctgaa aaaacgcccc
     4741 tccttctcgc ggcggctgta aaagaaggtc cggcccttga atccggcgcc ggccacccgg
     4801 ctctccgggt cgtcgtaatt gagcagcgcg atatcgtcgc cggcctggtt ttcgaaaatc
     4861 cggagcttgg ccttccggta agagccgaag tccggatagc ggtccaggtg gtcggcggtg
     4921 atgttgagca gcaacgagag tttcggccgg aagcgctcga tccactccag ctggaaactg
     4981 ctcacctcca ggatcaccgt gtcggttgcg gtcaggcggt caactaccga gatgaaggcg
     5041 ggccggatgt tgcccgagag gaccaccggc cggccggccg ctttcagcac gtcgcgcagc
     5101 agggtaacca cggtactctt gccgttggtc ccggtgaccg ccaggatcgg ggccgggcag
     5161 aaacggtagg ccagttccag ttcgctccag accgggatgc cgctttgaag ggcggcctgg
     5221 atcggccggc tctcgggcgg cacgccggga ctggtcacca ccaggtccag gccttccagg
     5281 aacgcctccg aatggccgcc cagttccacc cggacgccgg cggcctccag tttgccccgt
     5341 tccgtttcga ggccggggcc gcggcggttc tcggtcaggc ggacctcggc cccgcgtccg
     5401 gccaggaata atccgcaggc caggccgctt tcaccgagtc cgattaccag tatttttttt
     5461 ccggccagtt ccatgagtcg tcgatttcct tgatttctct acaatcttat catgtccccc
     5521 gccgccgtat caaccggcgg cgggccgggg agagagaggg ggtggagccc cttctcgcgg
     5581 tccgtggtcg gggaggggga gtcaggggag ggaagctcct tcccctgaaa gcgaagctcc
     5641 gccgccgtat caaccggcgg cgggccgggg tgagagaggg ggcggagccc ctttgttgag
     5701 ccgtgttttt tgtttttccc ttgagccgag ggtctggagt gagacgagtc gcggagccgc
     5761 ggcctcggga aagaagctta aaagcggcag cgctccggca tatcatcctc ccgcggctcc
     5821 tgtctgagcc aggccgcgac ggccgtcttt cggccccaag cggacagcga gttagccgcg
     5881 cgccggattt aagcgtacct ttcccccgcg gcaaagcgtc cgagccgaag ccagagccga
     5941 ggctcctgaa agcgaagctc cgccgccgta tcaaccggat tcgtctgaaa ttttcagaaa
     6001 caatagaggg ggccgtgaag cagagcgaag cacgcttcac ggcggtgaag tgtgttccga
     6061 gcttgtcgag ggactctact tcaccggaga gagggggcgg agccccttct cgcggttcgt
     6121 ggtcggggag ggggaatcag gggagggaag ctccttcagg ccatgcgttc caggtcccgt
     6181 tcggtaaccg gatagaccgg cggccggccg gagaaaaagt tctccagttc ccgcagcgcg
     6241 tatttcccga gccgtttcat gttgtcggtc acgcccccgg cgatgtgcgg agtcaggatg
     6301 acgttgggca gtttccagag aggcggttcc gggaccggcg gttccgggta acggacatcc
     6361 aggcaggcgg tcaggcggcc ggtcagcagt tcttcgtaga gggcggtttc atcgatcagg
     6421 gcgccgcggg cggtgttgat gagcagggcg ccgtccttga agagtttcag gttttcccgg
     6481 ttgatcaggt cgcgggttga gggcagggcc ggcgcgtgca gggagaggac gtcgacctgg
     6541 ggtatcagcg cctccaggct ggccgccggt tcgaccccca gccggaccgc ttcggccggg
     6601 tccaggtagg gatcataaag cagaatgcgc acctcgaagt tcttcaggag acggatgaag
     6661 tggcggccgg catggccggc cccgaccacg cccacggtaa ggccgtaaat ctcgcggatc
     6721 tttacccgct cttcctggtg ataccagccg ccctgatgca gcagttcgtt gaaacggcag
     6781 acccgcttga gggtgctcag catcaggccc agggtatgct cggccacgcc gatggcgatg
     6841 gccgccgagg cgctggtgac cacgatgccc cgcttccaga gggcttcgga aacaacgggg
     6901 cgtacgctgc cggcggcgta gacgaccagt ttcaggtccg gcgcggcggt caggatctcc
     6961 tcggtgaaac ggggactgcc ccagctggta agacaggcga cggtttcggc cggcaggcgg
     7021 ggcggctttt ccgggtccgg ttcgatttca tcgatccggg ccagccgatt cagccgttcc
     7081 cggtcggccg gactgaacag ctgggtctgg agctcttttt taatcaggga aacaacggtc
     7141 ggctttttca cccggcctcc ttttctgaat tattaaccgg cccggtattt ttcagggagc
     7201 cgggtaacgg tcccgggaga tgatctcgga gaaggcctcc cggccggcgc ggaaatagtc
     7261 gcgccgggtt acggtataaa accagccggc ccgtttttcg ggcggactgg ccttcaatct
     7321 tatttcccat tcgggggttt ccgttccata gaagccgatg ctcagagcgg ccgcctggac
     7381 ccgggcccgt atctggaggg ttgccgcctg ccggttccgg aaccgcaggt cggtccacgc
     7441 ctcggtactt acggccgcgt ccagtcccgg cggcaggtaa ccgaccaggg atgagtgcgg
     7501 gtgccgttcc tggatctgaa ggccggcggc cagggcggcg cagtagaggg tggaggagac
     7561 ctggcagagt ccgccgccgg gcgcgggccg gcggcggcct tccaccaggg aggaggcgta
     7621 gcccagggca ccgggtttca gctgccgggt aaccgtttcc aggaaggaaa actccgtttc
     7681 cggccccagt cggcagccgt ccagtttttc caccgccagg aggatgttgt gttcgcgggc
     7741 gtccaggccg gtttggaagg aggtggtgca gcttccccag cagtggacga aatcttcggc
     7801 ggccgccgga ccgccgccca ggagcagcag gccgagcagg agtagttttt tcattcttcc
     7861 cgcacccgga gatggaatcc gtcggaccgg ccgttgactt ccgggaagta catcagggag
     7921 gcccgggccg gccgcacctg gaagtcgccg gtcgtctccg gacggaggtg gtaaaccagg
     7981 gtccgggtgc ctttcccctc ggcatccagt ccccagaagg gggtgaagaa gacgatccgc
     8041 tcatcgtgca cttcacgccg gcagtaccag ttaccccccg cttcgttctc cggggtctcc
     8101 accacctcaa agccgctggg cagtgcgtct tcaatgacgg cgaattcgta gaagtcccgg
     8161 ccggtgatcg taagcgtgac ccggacccgt tctccgacct tcagggtgtc gccggccgcg
     8221 aacggcttct cctcagggtt tccggccgcg tccccggccc cggcttgcag gcgccggtac
     8281 tcccgcttga cggtgaaacc gcggtcctcc ggttcctgga gccggctgcg gtcaaggtaa
     8341 tccagggcgt gcgagtagta aaggttcccg gccccggttt tcttaaggga aagcgtattt
     8401 tcctctccgg ccttcagggt tccgggcggc gcggtgatta tgcggacaaa ctcccgaagc
     8461 gagtcacggt cgaacttttc ccgggccaaa ggctggttat tcaccgtcaa ttccgccgtg
     8521 aagtccgggt tgagctccgt cccgcggcgg aggaagtcgg tgagggccga gacgcagacg
     8581 gcggtatcct tggtggatcg ccagcggccg agccggcggc tggccaccag gtaccggatg
     8641 atctttggta tctccgggct ctgcggggcc agggccagga ccgccttcag ggcccaggcg
     8701 ctggcttcga cgtcgctgtc cacccaggag tagcggtccg gacggtccgc cttccagaag
     8761 gagatgtccg gggtcagcgg tttcaggtcg gccagcagtt cctccgccac ctgggcggcc
     8821 acccggccgg ccggatcggc tttgtgaaag gagagcgcca gcagggcccg gccgtaagga
     8881 ttgagccggg agcgttcctg gaagagtttg gccagggttc ccttcagtcc ggacggcacc
     8941 gccggctccg cttggctgag gctgaaggcg gcataggccc gggcgtccgg gtcctcgagg
     9001 ctcttgactt gttccgccag gaagcttacg gcccggcgcc ggacctcggg atcgaccggg
     9061 tagccgcact cttcggtcag ggtgaggccg tagaggacca gggcgctggt gtagggatgc
     9121 gaggcgtcgg ccgcccacca gccccagccg ccgtcggcgt gctggtaatc gtaaaggcgg
     9181 gccaggcccc ggttgaccat ggccggaagt tcttccagca tcttctggaa ggctttcggg
     9241 tcgtcgctca gcagccggag atcggagacg cccagctccc gaaccgcccg ggccacctgg
     9301 agcgtgggca ggaagcggtt caaggtctgt tccacgcacc cgtagggata cccggccagg
     9361 taatccaggg aacggaagag gccggaggcc agcgaaggat agaggtagct ggtcagtttc
     9421 gggctggcgg ccgccgccgg cagcttgaaa ctgttcaggg ccgactcccg gcactctccg
     9481 gaacggacct ccaggaccgc cgtggcgtaa ggaagcaccg gaatcttcgt ttccaggccg
     9541 tcggagtaat cgcggttgcc ggcccgcaaa gccagctcgg cttcgggtgc ctcgccggcg
     9601 acggccagcc actggcagcg gatcgaaccg ccggccggga tggtggcctc ttgcggcgcc
     9661 gtttccagca ggcggaggcc ccgcgcttcc aggatgaccg tcaccggctg gtcggcgccg
     9721 gtgtagttgt ggatcacggc cgaaagggta aaacggtcgc cttcgaccag gaagcggggg
     9781 gcggccaggc gggccagcag cggcttggag accagtatct cccgccgggc gccgcccacc
     9841 cgggtttcgg gcgtaaccgc ccgggcggtc gcccgccagg tggtgaggtt atcgggcagc
     9901 gccacttcca gggaggcccg cccctcgcgg tcggtccaga gcgccgggat ccagaaggcg
     9961 gtgtccttga aattccggcg cagaccggtt tcggccaggt ccttggcgcc gccgtactcc
    10021 caggtgtaga aggagtagcc ggtcgagacg cggttgggtt tgcggccgta aaagaaacgg
    10081 tccgggtcga gcgtcgtctc cggcgcgatg gcatagatgg ccttgtccac cagtccaagg
    10141 gagacctccg ccggggtgcc ggccccggcg ctgtccaggg tgcggatggc gaaccggctg
    10201 atctccccgg ggcggtattc ttttcggtcc ggagtgattt caaccgtgag aaagcggttc
    10261 ggttcgacca ccggcagggt ccgttccgcc tggtgcagtt cccggttgcg gaccgcggtc
    10321 aggccgaggg tgaaattcgg gatgtgttcg gccgttacgg ggaattccag caggctggtc
    10381 tgtgcctccg gctgcaggat ccggctttcg tggaggcggg ttccctcgac ggtgaggagg
    10441 atcggctgac cggggaaggc cgtcgtgatc agcacccggg ccgtatcgcc cggccggtaa
    10501 cttttttggt cggtcacgat ctccagttcc cggctcgaag accaggcgga ggtgtattcg
    10561 cggccggcca cccagatgta gcggaggccg ttgaccaggt ttttgtattc atctgaggcc
    10621 tgggcgtaaa ccttcaggta gccggaacgg ccgggtttca ggttcaggga ggtgcgcccc
    10681 tccggcccgg tgacggcggt ttcggcctgc agctccgtcc aggtgtagcg gtttttctcg
    10741 taacgttcca gggcgacgaa gaccttgatc ggaagtccgg ccaccggccg gtcatcccag
    10801 gttcggaccg ttatttccag ggggacggcc tcgcctggat aatagacgta gcgcggagtg
    10861 acgagcccga ttcgaaaggc gcccgggact gcgtccgccg aggcccgtcc gatcacctcc
    10921 cgctggctgc ggtcgacgac ccgggcctcc agggtgaaac gggtttcgtt ttcgtaggcg
    10981 ccccggcccg ggagttcgac caggcaggtt ccgtccggcc cggtctgggc ttctccctgc
    11041 cggagcagtt ttccataccc ccagcccggg cccccgtttt cattttcgta ctcttcgtaa
    11101 tcataatccg cccaccagcc ggatcgttcg taaaccaggt agttaacccg ggcgttggcc
    11161 accggctgtc cgaaatagta actggcccgg agccggaatc ggatcttctc gccgggcagg
    11221 tagcggtcgc ggtcggcctc gacctcgacc tggtactcgg gcttgcggta ttccaggacc
    11281 tgaaaaccgg aggttccgga ggcggcgatt gcttgatccg gatggattga aagcgtgtag
    11341 ttgcccagcc gcggctcggc ggccagttcc agcgagccgg ccagggagcc gttttcactc
    11401 accggcagcc aggtgtcgga gaccagccga tcctccgggt cacggacctg gacccgggcc
    11461 ttccgcgccg gcggcacctc gtaacggttg ccgtggtcaa ggcgcaggaa acccttgaag
    11521 tagacggtct ggccgggccg gtaaacgggc ctttccgtgt aaaggtaggc gatggtcggg
    11581 tcgctgcgga cgttcgacgg ccgggcggtc ggcaggttca gcagggccag cgaggttccc
    11641 gcttcggcca gcgccaggcc accgccgttc gtctccagcc ggccggaagc gtcggtccgg
    11701 cccagcagcc gcggcgcacc ggggccgccg agggtccaga tgagcgtttc gggccgcggc
    11761 cggccgtcgg ccaggccgca ggcgaagagc aggttctgct ccggggccga cttgcggacc
    11821 agggccaggt cggttaccag gcagtagaac ctgtcttccc cgccgggccg gtcgggaacc
    11881 tcctccggag aagtcagccg gaccaggtag aggccggccg gcaggctctt cagggtgacg
    11941 gaccggtcgt agaggtcgct gagcggctgg ggatagcgga ccgtgaagag gaggctggcc
    12001 gccggcggcc ggccggatag atcggcctgg cggatcatcc attccagggt gtgcggattt
    12061 aattgctgac ccaggatctt ttccgcctcg gcgcgcgggt tgaacggata gagttccagg
    12121 cggagtttgg ttacccggaa ggcattgtac ctgatctgga cgctctcccc gggctggaag
    12181 atatagttgc gcctattcag gctgaagctc ttgggccgga gctggaggcg tatttcgccc
    12241 ggcagccggg tggtctggcc cggccggacc tccaggttgg cggcgcttcc ctcccggtac
    12301 ccctgggtgt aaacggatat cttgtaagtg ccggccgcca ggccgtccag gagcaggttg
    12361 ccgtcggaat cggtctgggc cgaaaggtag gtcggtccct cggtgttgac ccaggcccgg
    12421 gcgatcggcc gcccggtttc ggcggcgatg acccggccgg agagacggcc gcccggctcc
    12481 aggttgaaat tgacgccgcc ggtctcctcc ccctccttga ccgggacgct ttcccggtac
    12541 tggcgtacga agcctggagc ctgggcgttc aggtaggcgg gacccggaga caggttcttc
    12601 aggaggtaga aaccgcggcc atcgcaggga gtgttgttta cccggccgcc ggccagcggc
    12661 cggccggatt tatcgtaaac gaagccgctg attgagccgg gccgccggag cgtcagccgc
    12721 tgaatgacct ctccgtactt ggcctggttg atctccaggg aacggaggtt ttcggccagg
    12781 tagccggcgg cctcggcccg gatgcggtag tagccggggc cggggacggc ggcctcggcc
    12841 cggccggtcc ggtcggtggt cgcggcggcg gtttccttcg cgtgcaggcg ggttacggtt
    12901 aaccgggcgc cggccaccgg ctggccgtcg gaacggtcaa cctggaagga aatggtatcg
    12961 gcccgcaggt tttcggccag ccagagcgaa ccggccagca gcagaaacag cgccactttt
    13021 cgactcgtct tcatttcgtc tcccgatact gaatggcctg gaaccggtat aactgcggcc
    13081 gggcttccgg cggcaggccg gcattgcgca ggcattttct gaaggcgtac tcgaccgtcc
    13141 gggcctcgcc gggcagaacg accccggagc ggccggactg gcggaccacc agcccgtccc
    13201 ggtcgggccg gtagcggtcg agcgagtcga tggcttccag gtcctgggga aaggtcagga
    13261 tgatggttat cccggccgcc tcctccggac cgacgggccg gtagcgcggg tcctgccagg
    13321 cggccacccg ggtgaagtgg gcgatctcct gccgcaggtc gggaaagagg gattcgaagg
    13381 agccggcgca gccgcggacc cggccgttcc ggagcagggt tacgaagacg gcccccggcc
    13441 ggttcaattc ggcgggcggc ttcccgggga gagggttgga agtcggggtg gaccagccgt
    13501 gattgagggc ctgccgggcc tcagccaggg ccattttccc gggaatcgga tccgggaaag
    13561 ccggcccggc ggcgactatc agcaggaata gcgacagcca gccgatccgc atggcctttt
    13621 gagggatcat ttcacctcga tacgcttgta aaccctggtg ccgccgtaga tttcctcaag
    13681 gtagcggacc atcaggtagg ggtccttgcc gtggattctt tcgaagaggt gtcccttgcc
    13741 ctttgataat ttccggtcct tgggcaggcc gttgggcagc aggttttcgg ttttgatcag
    13801 gtccagcgag gcggtttcga tggccgtgat atcctcggcg gccaggatgc cgatatccgg
    13861 caccaggctg ggcgtggaca tgccccagca atcgcagaag atggtgatat tcatcaggaa
    13921 gtttatgaag agcaggttct ccggcgcgaa cttcttcagg aaggccgaag tgaccagggc
    13981 caggccccgg gaaaagtctt cgaaacggcg attttccatg cggatggcct tcttcggaca
    14041 ggccagcacg cagtgctggc agtaggtgca gttgtgaaag aagaaaccga ttttcttctt
    14101 ctcccggttg atggtgatgg ccccgttggg acaggcccgg tggcacttca ggcagaagat
    14161 acacttatcg gcatcgtaga cgaggccgcc ctccagcccg tggattttgc gccgtgtctc
    14221 cgaggggacg attcccatgg cgatattctt gattgcgccg ccgaaaccgc agtcgccgtg
    14281 acccttaagg tgggccaggt caatgaagaa atcgctgtcc agcgcctcgc cggagaggag
    14341 cgcctcgtcc aggccccgga atccgatcgg ttctcggtag taataccggc cggactggcc
    14401 gaaacagggg atcaccgggc agcccagcac ctcgtgggtg tagccgcgca tgaaggcggt
    14461 cttggccgag ttgtccatga ccttcagatc cttggcgccg gccgtcttga gggcctcgac
    14521 cagcaggcgg acaaagaggg ggtggatggt ggtgtaaccc aggtggccgc ccaggtgcat
    14581 cttgatggtg acgctctttt ccttgacccg tttttccagg gcgagctttt ccagcatccg
    14641 cttgaacttg gccggcaggg tccggtccgg ttcggcgctt tcataggcga tatcggcaaa
    14701 gtaaatccgg gccggcgcgg attgggggcc cctggaagag gctggtttct tgcttctggt
    14761 catgttcggt ctccggaaag atcttgaggg tgatttaatg aaggccaggc aaaatatctt
    14821 acagaaaaaa cggccgcgat gtcaaatttt acaagggatt cgggggactg gtcaccgggt
    14881 gtgtttaagg aggtaaccga tggccgccgg caattcctcc aacaggtcgg aggccgccag
    14941 ggag
//
LOCUS       DAOQYN010000014        14884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_4482_length_14884_cov_4.983276, whole genome shotgun sequence.
ACCESSION   DAOQYN010000014 DAOQYN010000000
VERSION     DAOQYN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14884
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4482_length_14884_cov_4.983276"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKN80_01240"
     CDS             complement(<1..788)
                     /locus_tag="PKN80_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34672.1"
                     /translation="MMKDRKVWKKRVFRLAGFGLFLLLTAFLRPAAAEPPQVLLHAGF
                     EGSADASGRELVYPNRFSSKGEPLTLFEYEPGVVGRAARFTWTNGYPGVLYPAEGNLD
                     PARGTLSYWVRKTDPFYINQYYPNIWGLGNETMARANPLILVALNDPRAAAIEDGKWH
                     HCLYTWDSAEKKRRQYLDGELLAEDEYRSPYRAKELIIGYRLTGSMDEIAILDRPLDA
                     AEVKSAYAAYRQGKSPFTLPKPAKREYLYPLEIAALPSARPELPA"
     gene            complement(832..3726)
                     /locus_tag="PKN80_01245"
     CDS             complement(832..3726)
                     /locus_tag="PKN80_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34673.1"
                     /translation="MKRRLNFIRQRFLRPAALLAAVAILAAGLPSRASAEPPRNLLLK
                     STFDDAVRADFPHGWDYGRLPRFSATDGLPSPDRVELALNQAAARFGGQGADILLKVQ
                     QNFAFWFPLAQPLPVRKNEERLFLVRFWVRGDDRLEDGAFTRVAWSLPICNSQGSPVW
                     SEQKIRLEFIRLPGAPNFSVQGARAPQEIQYTYFRDWTMLGATLKATGLEDGHTLAGL
                     VFYTAKKGGDPTVPMRLQVDQVELFDITSLSDSEIGLLMQGMAESSSEERRTAILERE
                     AALRTKDSPGQPGNHIRNASFETNGDYQWILSGGSPINPEAYLAGNAWHGRRCLAFSR
                     LRTLAGMPSELIESPAIAVRPFRKMLLSAWARKEDPGKPARFSMAVTEGDFKADFDTA
                     LNGDWQRFSLPITVGSRYLGGLGFQVRGNNILLDALQLEEGTSATPFRDADAWSVALV
                     NDGRGNIQFLDQPFEVVLQAFASNQPAGRLSGRLVVTDLFGRSVAEVPFQREAPPGGW
                     LEEKVVFDRNLRGIFNVAFLREASNPLGLREASETVVARVPRPVEIPPLESIIGETPY
                     FNDFNLSLLERLGVKWARTIPSNISAFRWSQTETRPGVYDWSFADRIMEMVKNRPIAI
                     LGHLNGAPEFHGRPRSYSSNHGGFAPADMEAWSEFVSGAIRRYGSRVAAWEVWNEPSK
                     EDGKLYYNEAQAAAFKAVKQVDPKAFVVAWSMPESSGAAQRYASLTPGDEALLDGLDI
                     HIYGPANRGFLSGLKRGVGNRLGRIPVWNTECGLAQPASMYRYSGRDEVSSRVSQAAA
                     MAQLVLVHKAIDAKFFYYTSRFPHMASGLGGYSWGVIESTGVPSTGGVARAVAAHFVD
                     GGRTAGYVERDDAFGLVVRRDGRTRVALFTYAYTNEYPSKLNLAASLDLKLPAGLRGA
                     DIFNLMGQPQGKAGETLKVTYTPVYLDLGPADPEAALEALRTAMAPAAGK"
     gene            complement(3804..5699)
                     /locus_tag="PKN80_01250"
     CDS             complement(3804..5699)
                     /locus_tag="PKN80_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ34674.1"
                     /translation="MKKNDWLDRDGFLALETDAGKLTPDELVLYRRYRERVEGEINRL
                     NSGLGATGRVPEEPTDPEYVNPSVPRAFYYLDFPSPQTLIRGDDYYRMQNLAIGMPRD
                     GLESDPERLRQNAGRQLAPLCFRLESGWRLLGRMGTARKHLLVKKSPARLRLALPYPY
                     ESMLLFPGGRLAEGFWQGSLSLRLQPGKLKGEIRARSLRPGEGGLPVTFFLFDYDEFD
                     DSFLWYRQGEEQVQRWVDLGLEAGLKPPPAGQVHDCPVGALKGNQAFFLSRQGVGIRF
                     TLVSAREEGLERFFAESLAQFSRVNLAGINLEVAPGSLGLDVHYHSYPAKASLDFEWE
                     LECFDFSREKCRPVFDYPPLAPDRIRFVNSHAHIDYRRKLPDTVRTLRKNGISACLSG
                     IPLVTDSPGGLKIFGNWPVYAAMRTYPDVFRGFGHVQLNPEGYTNFPASPPDGPESVE
                     RLYRLGFSGLCVFEAGHPQVEVNAPDFHPIYRRAARLGLPMLFHNDPNPMLRSENPSA
                     SGQSIGHAARVARSFPELQILLTYVTIPTAGEHLRWVSLEHLVPLLEVFPNLLIQALQ
                     VNTAEMIAAVKDRGVIRKIVLGTDAPIRVNLAFIADFRRHLLKAGVSNSDIRWCCAEY
                     LRRPAGR"
     gene            complement(5844..7622)
                     /locus_tag="PKN80_01255"
     CDS             complement(5844..7622)
                     /locus_tag="PKN80_01255"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ34675.1"
                     /translation="MNKRLIFSLLALLLILTAGVAAAGSKPTADEAVLLERWQTLDRL
                     NQAMQKPYRESQAAKLLEVGPWRLAGLEAVASDTGPVGRSYLTIYFHAGERWLKVGGF
                     FIAGRALVEEAPGGKRIRMVFPHDRFNLSSGPKLQPGFWTGTQVITVTPDRIVNEYDL
                     TALAASGDMNVSLPYFDADRFDEAYLYTAAEGRHQVNLLDLGLESGLKEVPARRRPPA
                     WLGAADPASLALVSREGMGIKLSWRHQVEGARVVDDPGSPGRTYLRLAGPRFVVDAGR
                     FSGSFGLLDIQAGARVRVVSVMEGHYERDRRVVPPNRVYPPLDWRQARAFDSHGHFDQ
                     SMPMDDLIRVLRKNRIIFGVTSIEYLEGAGHRAFLGDQPVLEAMRRAPDVIVGFGFVR
                     LNPTGSYPNCPAAGRSGAADVERLKRLGFPGLKAIEKWSPANYDDPANDPVYAAAARL
                     RMPIVFHAGFDWSNNCSASRLAEVARRHPDLPAVAIAHGSEAADFDKLVEALRKTPNL
                     YQQHMHYGSVADLKRFREAGLAAKLVFATDNQTEATGEAAAAAGLIRNLRQAGYTEPE
                     IEFIMVGYAAGWLNEPRLRRSAAAGK"
     gene            complement(7861..9543)
                     /locus_tag="PKN80_01260"
     CDS             complement(7861..9543)
                     /locus_tag="PKN80_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ34676.1"
                     /translation="MKKRGSGWILIGLILLTLVGLPKAVVAFDKSDLPRMEKRLVFYT
                     RGSYALSCSQLIKLTAEEQAKKRFLTVNFYYIGSRGWHDPLFNQTIQLPVEVKETPEA
                     VVLTSPVSGVSYKVSAAGLEIDKSFKFENENSGGMLWYIHPDNIRFDDAVCYNRSGEK
                     LVTRLFKPYSITPADSVRDDTAAVLKFFEGQADFTPDFVVRTEEGLWTLWDFRLEGDL
                     GKVKTFFANQDPVRPMIGSPSTLLFYNTASGVQAGTALQTGLKLRVVEDQKFPWPVKA
                     ASASVTLIDTHVHLTTLTDLRDAALMARKHGLRYCILSFLYDERPYGRLFEGDPETFK
                     VMERYPDIFYGFGFIQLNTGGYPGFPRSGPDSPEKVTEQWQRGCKGIKIIEKWTSVAL
                     DDPQFDPLYKRMEELSLPLAFHTEPEGRGSDNIRAARVAEKFPGLPVIMAHLSTDTQL
                     EQIIPYFKRLPNLYIQHMHLSTVRTAEKKTALQKLIGAGLIRKVVFGSDVQDDYGTLL
                     SAGLFARQLLDMGVSQADVDAVMFRTAEELIRKVKPVPAGQAISGGGRPATK"
     gene            complement(9815..10837)
                     /locus_tag="PKN80_01265"
     CDS             complement(9815..10837)
                     /locus_tag="PKN80_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ34677.1"
                     /translation="MEDLNGQLDNFKYGDRFYTIADICRKYGVSQITAVKALSELEQQ
                     GLVQKIRRRGTIVTARKGSGRLWLIIPGVGGVEKSLYRSVVWMKIYQGLVSQAAKIGF
                     EFCGPISDANLTSLSLSGDKKAGFFVIRNLEPRIKGFLQEYSLPCVLLHPDIEDADTC
                     MVKVNRKRGGFLATEHLAGLGHKRIGLMIGSITSAPYLPRFQGYQQALRRAGLKFDWS
                     LVKETSGFDPAEDSYALDQILAKPDPPTAFFAANYSRALNLLQYCQLRKIRVPENLSI
                     AAYDNFPEMSISQPPLTTIETNLEEVGATGVNLMAALMRGERLQPEVVVETELVVRAS
                     ARPLKA"
     gene            complement(11001..12314)
                     /locus_tag="PKN80_01270"
     CDS             complement(11001..12314)
                     /locus_tag="PKN80_01270"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HNQ34678.1"
                     /translation="MKKVKIVIIGVGSLSFGRKAIVDVIANPELTGGAEVTLALVDTN
                     TAALETMRAMAERYREHFKVPGLKIEADPDRRRVLTGADYVITAVSRQRWPLWREDFT
                     IPAAFGFQQVLGENGGPGGAFHALRSIHLMLEISRDIEALAPGAWLLNYSNPESRVCL
                     AVSRLTRLKAVGLCHGAFTTVATAARILGRPESELDVEIGGINHFHWMLKVTERATGR
                     DLYPEFHQRLAEGDQGLDALTLQLYRTFGLLPFPTANHTGEYLSFAYETTGPTWMKYA
                     RPVPLEAGNEEVNATVFHFGKILRGEDDFNQEWVLKRSQELAAEIICDIEFDRNHKEV
                     SVNLPNQGAIPNLPAEAIVEVPALVGAAGIKPVAVGPLPEAIAALCRTQISIQTLLVD
                     AYEKRSRKLLLQALMLDPVVNSLPRAERLMDEMLRLQAAFLPEFE"
     gene            complement(12568..13380)
                     /locus_tag="PKN80_01275"
     CDS             complement(12568..13380)
                     /locus_tag="PKN80_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34679.1"
                     /translation="MTEKKPVFPKRREQKLLYDLTELKLWYVFDLVAAGRLDFEAALL
                     SQTTFYGLLPWFLSPPEKDTPQADQPLWRALVERLRPIHAAHAADRRAAAFCREAAAV
                     VFPLALAADIRHRMTNREEWFGCFRYDYTPENRLLRLHFKNAYVPDSPFADPARLFRT
                     LAEMIRDLEARGYAPESVTCGSWIHHLRPFQALFPASYLASLTPTSPDSKTGDGWWGQ
                     FTSRQGTLHRRRAKILKREGRFEFDRLSGRCPYRDYREHIMAHLHLKDGAGG"
     gene            complement(13377..14504)
                     /locus_tag="PKN80_01280"
     CDS             complement(13377..14504)
                     /locus_tag="PKN80_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ34680.1"
                     /translation="MTAREINLAVFEGRPVDRIPFQPRLETWYEWNRAGKPYGAPFPE
                     KYRGLSLRDIYDDLGAAPRYLYGFWPVEEVNDVEVRITVTENGDRRFRTIETPAGRLV
                     SEYGRSSEGGWRLVRHPVSDADEIESAIAFFRHTSYHFRPETFEAGSAFFGDRAQPQF
                     YLPRGGYQALSIVWMGLEGLIYNLADRPGRVEALMAAIDDSYDTLYRELAAYGRARLV
                     NFGENIDANIVSPRHFETYCLPFYEKRAGQLRAAGIRTHIHLDGSLRPLLKYLKFLPF
                     DGLEALTPRPQGDVELEELRAAAGGKVLLDGIPAISFLPSCPEAEFQATVERVVKLFA
                     PRLVLGASDEVPPAGEIERVRWAADYCRRALKPAASGPEKK"
     gene            complement(14583..>14884)
                     /locus_tag="PKN80_01285"
     CDS             complement(14583..>14884)
                     /locus_tag="PKN80_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34681.1"
                     /translation="PQVNFKGVRILLAENGRLASQGFAGARPADFERTLILFPSLPAG
                     VSFTNRDQGYGPVVRDLRIAYFDRSGRLLKVDLMRRIDGVSTPPPGTVVAIEGLP"
BASE COUNT         2683 a         4568 c         5037 g         2596 t
ORIGIN      
        1 gccggcagtt cgggccgggc ggagggaagc gccgcgatct ccagggggta aaggtactcc
       61 cgtttggcgg gtttgggcag cgtaaagggc gatttcccct ggcggtaggc ggcgtaggcg
      121 gacttgacct cggccgcgtc cagaggccgg tcgaggatgg cgatctcgtc catggaaccg
      181 gtgagccggt agccgatgat cagttccttc gcccggtaag gcgaccggta ttcgtcttcg
      241 gccagcagtt cgccgtccag gtactgacgc cgcttctttt cggcgctgtc ccaggtgtaa
      301 aggcagtggt gccacttccc gtcctcgatc gcggccgccc tgggatcatt cagggccacc
      361 aggatcaacg ggttggcccg ggccatcgtt tcgttgccca ggccccagat gttcggatag
      421 tactggttga tgtaaaaggg atcggtcttc cgcacccagt aggagagcgt gccgcgggcc
      481 ggatcaaggt taccctcggc cgggtagaga acgccgggat agccgttggt ccaggtgaaa
      541 cgggccgccc ggccgacaac gccgggttcg tattcgaaga gggtcagcgg ttcgcccttg
      601 ctgctgaacc ggtttggata aaccagctcc cggccggaag cgtcggcgct gccctcgaaa
      661 ccggcgtgca gcaggacctg cggcggctcg gcggccgccg gccgcaggaa ggcggtcagc
      721 agcagaaaga ggccgaagcc ggccagccgg aaaacccgtt tcttccagac cttgcgatcc
      781 ttcatcattc catacctccc gtgaatcggt taaccgctga tcaataatga tttactttcc
      841 cgcggccggc gccatggccg tccgcagggc ttccagggcc gcctccggat cggccgggcc
      901 caggtcaaga taaaccgggg tgtaggttac tttcagcgtt tcgccggcct tcccctgggg
      961 ctgccccatt aaattaaaga tatccgcgcc ccgcaacccg gccggcagct tcaggtccag
     1021 gctggccgcg aggttcagtt tcgaggggta ctcgttggtg taggcgtagg tgaagagggc
     1081 gacccgggtc cggccgtcgc gccggacgac caggccgaag gcgtcgtccc gctcgacata
     1141 gccggcggtg cgaccgccgt ccacgaagtg ggcggccacc gcccgggcca ccccgccggt
     1201 cgaggggaca ccggtggact cgatcacgcc ccagctgtag ccgcccaggc ccgaggccat
     1261 atgcggaaaa cgactggtgt agtagaaaaa cttggcgtcg atcgccttgt gcaccaggac
     1321 cagctgggcc atggccgccg cctggcttac ccggctgctg acctcgtccc ggccgctgta
     1381 gcggtacatg ctggccggct gggccaggcc gcactcggtg ttccagaccg ggatccggcc
     1441 cagccggttg ccgactccgc gcttcagacc ggacaggaaa ccgcggttgg ccggaccgta
     1501 aatatggata tccagcccat ccagcagcgc ctcatcaccc ggcgtcaaac tggcgtagcg
     1561 ttgggcggcg ccgctggact cgggcatcga ccaggcgacc acgaaggcct tcgggtcaac
     1621 ctgcttgacc gccttgaagg cggccgcctg ggcctcgttg tagtaaagtt taccgtcctc
     1681 cttgctcggc tcgttccaga cctcccaggc cgccacccgg ctgccgtagc ggcggatcgc
     1741 cccggaaacg aactccgacc aggcctccat gtccgccggc gcgaaaccgc cgtggttgga
     1801 gctgtagctg cgcggccggc cgtgaaactc gggcgcgccg ttgaggtgac cgagaatcgc
     1861 gatcggccgg ttcttaacca tctccatgat ccggtcggcg aagctccagt cgtaaacgcc
     1921 gggcctggtc tcggtctggc tccagcggaa ggccgagatg ttgctgggga tggtccgggc
     1981 ccacttcacg cccagccgct ccagcaggga aaggttgaaa tcgttgaagt agggcgtctc
     2041 gccgatgatc gactccaggg gcggaatctc gaccggccgg ggcacgcggg ccaccaccgt
     2101 ctcggaggcc tcgcgcaatc ccagcgggtt ggacgcctcg cgcaaaaaag cgacattaaa
     2161 gattccccgc aggttgcggt cgaagacgac cttctcctcc agccagccgc ccggcggcgc
     2221 ctcgcgctga aagggaactt cggccacgct ccggccgaag aggtcggtca ccaccagccg
     2281 gcccgaaagg cggccggccg gctgattgga ggcgaaggcc tggaggacaa cctcgaaggg
     2341 ctgatcgaga aactggatat tgccgcggcc gtcgttaacc agggccaccg accaggcgtc
     2401 ggcatcccgg aagggcgtgg ccgaagttcc ctcctccagc tggagagcgt ccagcaggat
     2461 attgttcccg cgcacctgga agcccaggcc gcccaggtag cggctgccga ccgtgatggg
     2521 cagtgaaaaa cgctgccagt cgccgttgag ggcggtgtca aagtcggcct tgaaatcgcc
     2581 ttcggtcacc gccatcgaaa accgggccgg ttttccggga tcttccttgc gggcccaggc
     2641 ggagagaagc atcttccgga agggacggac cgcgatcgcc ggcgattcaa tcagttccga
     2701 gggcatcccg gccagggtgc gcaaccggga gaaggccagg cagcggcggc cgtgccaggc
     2761 gttgccggcc aggtaggcct ccgggttgat cgggctgccg ccggagagga tccactggta
     2821 gtcgccgttg gtttcgaagg aggcgttgcg gatgtggtta ccgggctggc ccgggctgtc
     2881 cttggtccgc agggccgcct cccgctccag gatggccgtc cgtctctcct cggaactgct
     2941 ctcggccatg ccctgcatca ggaggccgat ctccgaatcg gagaggctgg tgatgtcgaa
     3001 gagttccacc tggtcgacct ggaggcgcat cgggacggtc ggatcaccgc ccttcttggc
     3061 cgtgtagaag accaggccgg ccagggtgtg gccgtcctcc aggccggtgg ccttgagggt
     3121 ggcccccagc atcgtccagt cgcggaagta ggtgtactgg atctcctggg gagcccgggc
     3181 gccctggacc gagaagttcg gcgcgcccgg cagccggatg aattcgagcc tgatcttctg
     3241 ctcactccag accgggcttc cctgggaatt gcagatcggc agggaccagg cgacccgggt
     3301 gaaggcgccg tcctcgagcc ggtcatcgcc ccgaacccag aaacggacca ggaagaggcg
     3361 ttcctcgttc ttccggaccg gcaggggctg ggcgagcgga aaccagaagg cgaagttctg
     3421 ctggaccttg agcaggatat cggccccctg gccgccgaac cgggccgccg cctggttgag
     3481 ggccagttcc acccggtccg ggctgggcag gccgtcggtg gccgagaagc gcggcagccg
     3541 gccgtaatcc cacccgtggg ggaaatcggc ccggaccgcg tcgtcaaagg ttgacttcag
     3601 caggaggttc cggggcggct cggcggacgc ccgggatggg aggccggcgg ccaggatggc
     3661 aacggccgcc agcagggcgg ccggccgaag aaacctttga cggataaaat tcaatcgtct
     3721 cttcatgacg ctcttttcgg atgcggaaac aaaccatccg aggggacgga ccctgaaaaa
     3781 aacctgaacc gggcctggcc cggtcaacgc ccggccggcc ggcgcagata ctcggcgcag
     3841 caccaccgga tgtccgagtt agaaaccccg gccttcaaaa ggtggcggcg gaaatcggcg
     3901 ataaaagcca ggttgaccct gatcggggca tcggtaccga gaacgatctt ccggatcacc
     3961 ccgcggtcct tgacggccgc gatcatctcc gcggtgttga cctgcagggc ctgtatgaga
     4021 aggttgggaa aaacctccag gagcggcacc aggtgttcca gggataccca gcgcaggtgt
     4081 tcgccggccg tcggtatggt gacgtaggtc agcaggatct ggagctcggg aaaacttcgg
     4141 gccactcttg ccgcgtggcc gatcgattga cccgaggcgc tcgggttctc cgaacgcagc
     4201 atcgggttgg gatcgttgtg aaagagcatc ggcaggccca gccgggccgc cctccggtaa
     4261 atcgggtgga agtccggcgc gttgacttcg acctggggat ggccggcctc gaagacgcac
     4321 aggccgctga aacccaaccg gtaaaggcgc tccaccgatt ccgggccgtc gggcgggctg
     4381 gccggaaagt tggtgtatcc ctcgggattc agctggacat ggccgaagcc tcgaaaaacg
     4441 tcgggatagg tgcgcatcgc cgcgtaaacc ggccagttcc cgaaaatctt gaggccgccc
     4501 ggcgaatcgg ttaccagggg tatcccggag aggcaggccg aaatcccgtt cttgcgcagc
     4561 gtccggaccg tgtccggcaa cttgcgccgg taatcgatgt gggcgtggct gttgacaaaa
     4621 cgaatccgat ccggggccag gggtggataa tcaaagaccg gccggcactt ctcccggctg
     4681 aaatcgaagc actccaattc ccactcgaaa tccagggacg ccttggcggg ataggagtgg
     4741 tagtggacgt caagtcccag cgaaccgggc gccacttcga ggttgattcc ggccaggttg
     4801 acccgcgaaa actgggccag gctctcggca aaaaagcgct ccaggccttc ttctcgggcg
     4861 gaaaccaggg taaagcgaat cccgactccc tgcctggaaa ggaagaaggc ctggttcccc
     4921 ttcagggctc cgaccgggca atcgtgaacc tgcccggccg gcggcggctt caggccggcc
     4981 tccaggccga gatcgaccca gcgctgaacc tgttcctcgc cctgccggta ccagaggaaa
     5041 gaatcgtcga actcgtcgta atcgaagaga aaaaaggtga ccggcagtcc gccctcgccc
     5101 ggccggagcg accgggcccg tatctcccct ttcagcttcc cgggctgcaa ccggagcgag
     5161 aggcttccct gccagaaacc ttccgccagc cggccgccgg gaaacagcag catggactcg
     5221 taaggatatg ggagggcgag ccggagccgg gccggactct tcttgaccag cagatgctta
     5281 cgggcggttc ccatccggcc cagcagccgc cagccgcttt ccagccggaa acagagcggg
     5341 gcgagctgac ggccggcgtt ctggcgaagg cgttcggggt ccgactccag tccgtcccgg
     5401 ggcatcccga tggccaggtt ctgcatccgg tagtaatcat cgccgcgaat cagggtctgc
     5461 ggacttggaa aatcaaggta gtaaaaggcc cggggaaccg acgggttgac gtactccgga
     5521 tcggtcggct cctccggaac gcggccggtc gcccccaacc cggagttgag gcggttgatc
     5581 tctccctcca cccgttcccg gtaccggcgg tagagaacca gttcgtcggg ggtcagtttc
     5641 ccggcatccg tttccagggc caggaacccg tcccgatcca gccagtcgtt ctttttcatt
     5701 gcggctcccg gattattgag ccagggggtg tcggccacgg cggccgtctc tcttttccag
     5761 gataatatta tccctaattt ccccggcgcc tgtctaaccc ggcccggacc ggtcttttaa
     5821 aagcccccgg gaagcgcgac tccttacttg ccggccgcgg ccgaacgccg caggcggggt
     5881 tcgttcaacc agccggccgc gtatccgacc atgatgaact caatctccgg ttcggtgtag
     5941 ccggcctgcc tcaggttcct gatcaatccg gccgccgcgg ccgcctcgcc cgtcgcctcg
     6001 gtctggttgt cggtggcgaa aaccagcttg gcggccaggc ccgcctcccg gaaacgcttc
     6061 aggtcggcca ccgatccgta atgcatgtgc tgctggtaaa gattcggggt tttccggagc
     6121 gcctcgacca gtttatcaaa atcggccgcc tcgctgccgt gggcgatggc caccgccggc
     6181 aggtccggat ggcgccgggc cacctcggct agccggctgg cgctgcagtt gttggaccag
     6241 tcgaaaccgg cgtggaagac gatcggcatc cgaaggcggg ccgccgccgc gtagaccggg
     6301 tcattggccg ggtcgtcgta attggccggg gaccacttct cgatcgcctt caaaccagga
     6361 aaaccgagcc gctttaaacg ttcgacatcg gcggcgccgg agcggccggc ggccgggcag
     6421 ttgggatagg aaccggtcgg gttgagccgc acgaagccga aaccgacgat cacgtccggc
     6481 gcccggcgca tcgcttccag gaccggctgg tcacccagga aggcgcggtg gccagcccct
     6541 tccaggtatt cgatggaagt gaccccgaag atgatccggt tcttgcgcag gacccggatg
     6601 aggtcgtcca tcggcatcga ctggtcgaag tggccgtgcg aatcgaaggc gcgggcctgg
     6661 cgccagtcga ggggcggata gacccggttg ggcggcacca cccggcggtc gcgctcgtaa
     6721 tggccttcca tgacgctgac gacccggacc cgggcgccgg cctggatatc cagcaaaccg
     6781 aacgaaccgc taaagcggcc ggcatccacg acgaaacggg gaccggccag gcgaaggtag
     6841 gtccggcccg gcgaaccggg atcatccacc acccgggctc cctccacctg gtggcgccag
     6901 gataatttta ttcccatccc ctcgcgcgaa acgagcgcca gcgaggcggg atccgccgcg
     6961 cccagccagg ccggcggccg ccgccgggcc ggaacctcct tcaacccgct ctccaggccc
     7021 aggtcgagca aatttacttg atgccggcct tcggcggcgg tgtagaggta ggcctcgtca
     7081 aaacggtcgg cgtcgaagta gggaagcgaa acgttcatat cgccgctggc ggccagggcg
     7141 gttaaatcat actcgttgac gatccggtcc ggggtgacgg tgatgacctg ggtgccggtc
     7201 cagaaaccgg gctgcagctt ggggccgctg ctgaggttga agcggtcatg cggaaagacc
     7261 atccgaattc gcttgccacc gggcgcctcc tcaaccaagg cccggccggc gatgaaaaag
     7321 ccgcccactt tcagccagcg ttcgccggcg tgaaagtaaa tggtgaggta ggagcggccg
     7381 accggcccgg tatcgctcgc gaccgcctca aggccggcca gccgccaggg gccgacttcc
     7441 agcagtttgg ccgcctggct ctcccggtag ggcttttgca tggcctggtt gagccggtcg
     7501 agcgtctgcc agcgctccag caggaccgcc tcgtcggcgg tcggtttcga cccggccgcg
     7561 gccacgccgg cggtaagaat taataataaa gccagcagtg aaaatatcag ccgtttattc
     7621 atgggatccc tccttctgat aaaccgttgg cggcaatcca gcattagctt gtcattcccg
     7681 cattccactt cgttcgtcat tcccgcaggt agtaggggag ggaatccagg tgtttatcag
     7741 ttaaaatctt ttaaagactg gatgcccgtc tacaacacac gggcatgacg tgcttcttgg
     7801 atacccgcct tcccgcctgc gggaacaggc acgcggtcat gacgatcaca aaaaacggcc
     7861 ttacttcgtc gccggacggc cgccgccgga gatcgcctgg ccggccggaa ccggcttgac
     7921 cttccggatc aactcctcgg cggtccggaa catcaccgcg tccacatccg cctgggagac
     7981 gcccatatcg agcagctgcc gggcaaagag tccggccgag agcaaggtgc cgtaatcatc
     8041 ctggacatcg ctgccgaaga ccaccttgcg gatcagcccg gccccgatca gcttctggag
     8101 cgcggtcttc ttctccgcgg tccggaccgt ggacaggtgc atgtgctgga tgtagaggtt
     8161 gggcaggcgc ttgaagtagg gtatgatctg ctccagctgg gtatcggtgc tgaggtgggc
     8221 catgatgacc ggcaggccgg gaaacttctc ggccacccgg gccgcccgga tgttgtccga
     8281 accgcgcccc tccggctcgg tgtggaaggc cagcggcagg gagagctcct ccatccgctt
     8341 gtagagcggg tcgaactgcg ggtcgtccag ggccaccgaa gtccactttt cgatgatctt
     8401 gatccccttg cagccccgct gccattgttc ggtgaccttc tccgggctgt ccggcccgct
     8461 gcgcgggaag cccgggtagc cgccggtatt gagctggatg aagccgaagc cgtagaagat
     8521 gtccgggtac cgctccatga ccttgaacgt ttccggatcg ccctcaaaga ggcggccata
     8581 ggggcgttcg tcatacagga aagacaggat gcagtaacgg agaccgtgct tgcgggccat
     8641 cagggccgca tcgcgcaggt cggtcagcgt ggtgagatgc acgtgggtat cgatcagggt
     8701 aaccgaagcg gaggcggcct tgaccggcca ggggaatttc tggtcctcaa ccacccggag
     8761 cttcaggccg gtctgcagcg cggtcccggc ctggacgccg ctggcggtgt tgtagaaaag
     8821 cagggtgctg ggcgaaccga tcatcggccg caccgggtcc tggttggcga agaaggtctt
     8881 caccttgccc agatctccct cgaggcggaa gtcccagagc gtccagaggc cctcctcggt
     8941 ccggaccacg aaatcgggag tgaagtccgc ctgcccctcg aagaacttga gcaccgcggc
     9001 cgtatcgtcg cggaccgagt cggccggcgt gatcgagtag ggcttgaaga ggcgggtcac
     9061 cagcttctcc ccggagcggt tgtagcagac cgcgtcgtcg aagcggatgt tgtccgggtg
     9121 gatgtaccag agcatgccgc cggagttctc gttttcaaac ttgaaggact tgtcgatctc
     9181 cagtcccgcg gcgctgacct tgtaggatac cccgctgacc gggctggtca gcacgaccgc
     9241 ttccggcgtc tccttcacct cgaccggcag ctggatggtc tggttaaaga gcgggtcgtg
     9301 ccagccccgg ctgccgatat agtaaaagtt gaccgtcagg aatcgcttct tcgcctgctc
     9361 ctcggccgtc agtttgatca gctggctgca ggacagggcg taggaaccgc gggtgtagaa
     9421 gaccaggcgc ttctccatcc ggggcaggtc cgatttatca aaggcgacga ccgccttcgg
     9481 caatccgacc agcgtcaaga ggatcaatcc gatcaggatc cagccgcttc ctcgcttttt
     9541 catgctccct cctttaaatg attagttgcc ggcaacgaag aaaaagatta aggggcgccg
     9601 aggcccggac atccggggaa agcggtcggt tcccggcgga tatggctgat ggggatgaag
     9661 aataatattt tagtatttaa aaaccccata ccccgcttca aaaaaatatg tgttaatttt
     9721 acctttcagg taatggagtg tcaatagatg gaaatatgaa atgtaaatta atggagaggc
     9781 gcggaaggaa gggccgccga ccagccggcc gccttcaggc ctttaagggc cgggcgctgg
     9841 cccggacgac cagctccgtt tcgacgacca cttccggctg gagccgctcg ccccgcatga
     9901 gcgcggccat caggttgacg ccggtggcgc cgacctcttc gaggttggtc tcgatggtcg
     9961 tcagcggcgg ctgcgaaatg gacatctccg ggaagttgtc ataggccgcg atgctcaggt
    10021 tttccggcac ccggatcttg cgcagctggc agtactggag caggttgagg gcccggctgt
    10081 agttggcggc aaaaaaagcg gtgggcggat cgggtttggc cagaatctgg tccagcgcgt
    10141 aagagtcctc ggccgggtca aaaccggagg tctccttgac cagggaccag tcgaacttca
    10201 ggccggcccg gcggagcgcc tgctggtaac cctgaaatct tggcaggtaa ggggccgagg
    10261 tgatggagcc gatcatgagg ccgatgcgct tgtggcccag gccggccagg tgctcggtgg
    10321 ccaggaagcc gccccgcttg cggttcacct ttaccatgca ggtatcggca tcctcgatgt
    10381 cgggatgaag caggacgcag ggcagggaat attcctgcag aaaccccttg atccggggct
    10441 cgagattgcg gatgacgaag aaaccggcct tcttatcccc gctcaacgac aggctggtca
    10501 ggttggcatc ggagatcggc ccgcagaact cgaagccgat cttggccgcc tggctgacca
    10561 ggccctggta gatcttcatc cagaccacgc tccggtaaag ggatttttca accccgccga
    10621 ctcccgggat gatcagccag aggcggccgc tccccttgcg ggccgtgacg atggtcccgc
    10681 gccggcgtat cttctggacc agcccctgct gctccagctc ggagagggcc ttgaccgcgg
    10741 ttatctggct gaccccgtac ttacggcaga tatcggcgat ggtatagaaa cggtcgccgt
    10801 atttgaagtt gtcgagttgg ccgttcaggt cctccatgac cgacttccag agcgacttat
    10861 gccttttatt aatattcttt tccatatttt caattttaca taatcaaaaa tctcttgtca
    10921 aaatattata tggaatataa aggcagggtt aaaatcaatt cgctatttaa catcaaaaaa
    10981 tccgaccggc gcccaaaaaa tcactcgaat tcgggcagga aggcggcctg gaggcggagc
    11041 atctcatcca tcagccgctc ggcccggggc aggctgttga cgaccgggtc gagcatcagg
    11101 gcctggagca ggagtttcct ggaacgcttt tcgtaggcgt ccaccagcag ggtctggatg
    11161 gagatctggg tccggcagag ggcggcgatc gcctccggga gcgggccgac cgccaccggc
    11221 ttgatgccgg cggcgccgac cagggccggc acctcgacga tggcctcggc cggcaggttg
    11281 gggatggcgc cctggttggg caggttgacc gaaacctcct tgtggttgcg gtcgaactcg
    11341 atgtcgcaga tgatttcggc ggccagttcc tggctgcgct tgagcaccca ctcctggttg
    11401 aaatcgtcct ccccgcgcag gatcttgccg aagtggaaga cggtcgcatt gacctcttcg
    11461 ttcccggcct cgagggggac cggccgggcg tatttcatcc aggtcgggcc ggtcgtttcg
    11521 taggcgaagg aaaggtactc gccggtgtgg ttcgcggtcg ggaagggcag caggccgaag
    11581 gtgcggtaga gctggagggt gagggcgtcc aggccctggt cgccttcggc caggcgctgg
    11641 tgaaattccg ggtagaggtc gcggccggtg gcccgctcgg tcaccttgag catccagtgg
    11701 aagtggttga tgccgccgat ttcgacgtcg agctcggact cggggcggcc caggatgcgg
    11761 gcggcggtgg cgacggtggt gaaggcgccg tggcagaggc cgaccgcctt gaggcgggtg
    11821 aggcggctga cggccaggca gacccgggat tccgggttgg agtagttgag cagccaggcg
    11881 ccgggggcga gcgcctcgat gtcgcggctg atctccagca tcaggtggat ggaacgcagg
    11941 gcgtggaagg cgccgccggg gccgccgttc tcgcccagaa cctgctggaa gccgaaggcg
    12001 gccgggatgg tgaaatcctc gcgccagagc ggccagcgct ggcgggagac ggcggtgatg
    12061 acgtagtcgg cgccggtcag gacgcggcgg cggtcgggat cggcctcgat cttgaggccg
    12121 gggaccttga agtgctcccg gtagcgctcg gccatggcgc gcatggtctc gagggccgcg
    12181 gtgttggtgt cgaccagggc cagggtcact tcggcgccgc cggtcagttc ggggttggcg
    12241 atgacgtcaa cgatggcctt gcggccgaag ctgagactgc ccacgccgat gatgacgatc
    12301 ttgaccttct tcattccgct ctcctcccgt gaagtcgttc gttaatgatt ttctttcagc
    12361 ccgaccaggg tattacctaa attcaaaacc ttttaaggac tggatacccg cctactacct
    12421 gcgggcatga cgggcttctt ggatacccgc ctactacctg cgggtatgac acacgtttta
    12481 gatacccgcc tactatcctc ctacgctgaa aaatgagctt cggcaaacaa gtccgcgggc
    12541 atgacatata aaaacattcc tgctggttta cccgccggcg ccgtctttca aatgcagatg
    12601 ggccataata tgctcccggt agtcccggta gggacagcgg ccggagagcc ggtcgaattc
    12661 gaacctcccc tcgcgcttga ggatcttcgc ccgccgccgg tgcagggtgc cctggcggct
    12721 ggtgaactgg ccccaccagc cgtcgccggt cttggagtcg ggcgaggtcg gcgtcagcga
    12781 ggccaggtaa ctggccggaa agagggcctg gaaggggcgc aggtgatgaa tccaggagcc
    12841 gcaggtgaca ctctccggag cgtagccgcg ggcctcgagg tcgcggatca tctcggccag
    12901 ggtgcggaag agacgggccg ggtcggcgaa gggagagtcg ggcacgtagg cgttcttgaa
    12961 gtggaggcgg agcaggcggt tctccggggt gtagtcgtag cggaagcagc cgaaccactc
    13021 ctcccggttg gtcatccggt ggcggatgtc ggcggccagg gcgagcggga agacgacggc
    13081 ggccgcctcg cggcagaagg ccgcggcccg gcggtcggcg gcatgggcgg cgtggatggg
    13141 gcggaggcgt tcgaccaggg cgcgccagag cggctgatcg gcctggggcg tatccttttc
    13201 cggcggcgag aggaaccagg gcaacaggcc gtagaaggtg gtctggctga gcagggccgc
    13261 ttcgaagtcg aggcggccgg cggcgaccag gtcgaagacg taccagagct tcagttcggt
    13321 caggtcgtag aggagtttct gttcgcgccg cttcggaaag acgggtttct tttcggtcat
    13381 tttttctccg ggccggacgc ggccggtttc aaggcgcggc ggcagtagtc ggccgcccag
    13441 cgcacccgct cgatctcgcc ggccggcggc acctcgtcgg aggcgcccag gaccaggcgg
    13501 ggggcgaaga gtttcacgac gcgctcgacg gtcgcctgga actcggcctc cgggcaggaa
    13561 ggcaaaaagg agatggccgg tatgccgtcc agcaggacct tgccgccggc ggccgcccgc
    13621 agttcttcca gttcgacgtc gccctggggc cggggcgtga gcgcctccag gccgtcaaag
    13681 ggcagaaatt tcagatattt aagcaggggt cgcaggctgc cgtcaaggtg gatgtgggtg
    13741 cggatgccgg cggcgcgcag ctggccggcc cgcttctcgt agaagggcag gcagtaggtt
    13801 tcgaagtggc gcggggagac gatgttggcg tcgatgttct cgccgaagtt gaccaggcgg
    13861 gcccggccgt aagcggccag ttcccggtag agggtgtcgt aggagtcgtc gatggccgcc
    13921 atcagcgcct cgacccggcc gggccggtcg gccaggttgt agatgagccc ttccaggccc
    13981 atccagacga tggagagggc ctggtagccg ccgcgaggca ggtagaactg gggctgagcc
    14041 cggtcgccga agaaggcgct gccggcctcg aaggtttcgg gacggaagtg gtaggaggtg
    14101 tggcggaaga aggcgatggc cgactcgatc tcatcggcgt cggaaaccgg gtggcgcacc
    14161 agccgccagc cgccctcgga ggagcggccg tactcgctca ccagccggcc ggccggggtc
    14221 tcgatggtgc ggaaccggcg gtcgccgttc tcggtgacgg tgatccggac ttcgacgtcg
    14281 ttgacctcct cgaccggcca gaagccgtag aggtagcggg gcgcggcgcc caggtcatcg
    14341 tagatgtcgc gcagggagag gcctcggtac ttctccggga agggcgcgcc atagggcttg
    14401 ccggcccggt tccactcgta ccaggtctcc agccggggct gaaagggaat ccggtccacg
    14461 ggccgccctt cgaagacggc caggttgatt tcgcgggcgg tcattggcac tccgtttgaa
    14521 cgggatgatt taataggcat ccaatcctta aaaggtttta aaccaaaaac aatctccctc
    14581 cgttacggca ggccctcgat ggcgaccacg gtgcccggcg gcggggtgga gaccccgtca
    14641 atccggcgca tgaggtcaac cttgagcagg cggccggagc ggtcaaaata ggcgatccgc
    14701 aggtcgcgga ccaccgggcc gtagccctgg tcgcggttgg tgaaggagac gccggccggc
    14761 agggagggga aaaggatcag ggtccgctca aaatcggcgg gccgggcgcc ggcgaagccc
    14821 tggctggcca ggcggccgtt ctcggccagc agaatccgga cacccttgaa gttgacttgg
    14881 ggcc
//
LOCUS       DAOQYN010000015        14844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_4501_length_14844_cov_4.300764, whole genome shotgun sequence.
ACCESSION   DAOQYN010000015 DAOQYN010000000
VERSION     DAOQYN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14844
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4501_length_14844_cov_4.300764"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PKN80_01290"
     CDS             complement(<1..191)
                     /locus_tag="PKN80_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNQ34682.1"
                     /translation="MIYWQLILTFLKIGLFAFGGGYAVLPLIQRELVVNHRWLTDGEF
                     SRLVSVAEMTPGPIGINSST"
     gene            complement(188..748)
                     /locus_tag="PKN80_01295"
     CDS             complement(188..748)
                     /locus_tag="PKN80_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNQ34683.1"
                     /translation="MENRIRLNGWHFFLAFFKIGGFTIGGGYAMLPLVREMVVRRKGW
                     ISEERFLELLTIAQGTPGVMMVNLSTLIGLELGGLRVALLAVSGAILPSFLSILLIAS
                     VLSRFMGNPAVLAFFRGAVPAVVGIIGGVVLQLAWRHLRRPESILAALAALGLLIGLG
                     VNPILVILAALLLIGLKLKLASGGGR"
     gene            933..1940
                     /gene="amrS"
                     /locus_tag="PKN80_01300"
     CDS             933..1940
                     /gene="amrS"
                     /locus_tag="PKN80_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNQ34684.1"
                     /translation="MTEARFYDSLAGGRVECRLCPHHCRIAPDQTGRCGVRRNQAGKL
                     YSLVYGRPVAERADPIEKKPLFHFLPGSTSFSVATAGCNLTCRFCQNSDISQVNGRII
                     YERELAPAEIVERARAAGCRSVSYTYTEPTVFFEYVFDTARLAAGAGLRNVLVTNGYL
                     EADPLRELAPYLDAANVDLKSFRDEYYRNFCGGRLRPVLETLELMRRLGIWVEVTTLL
                     VPGENDGAGELREAADFLSGRLGPDTPWHLSRFFPHYRFMNAPPTPPETLAAAREIGR
                     AAGLHYVYTGNLPGDPGENTACPQCGRQVIGRTGFLVTGYALTAGRCAACGRSIAGVF
                     D"
     gene            complement(1884..2477)
                     /locus_tag="PKN80_01305"
     CDS             complement(1884..2477)
                     /locus_tag="PKN80_01305"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNQ34685.1"
                     /translation="MGRIRPPEPVLLFVGLLGADPALFPSLKSQLTRRFGPLLTESRL
                     LDFRCSDYYERELGAGLKRQLLLFRRPFPPGRLAAAKVFTNRLEEAWSQAGRRRVNID
                     PGCLYPARLVLASTKDYSHRIYLGRGIYGEVTLVYRDGEFQALPWTYPDYRSEYYRRF
                     FGQARPFLLERLRVGRRAASVKDAGDGPAAGGAAAGG"
     gene            complement(2455..3132)
                     /locus_tag="PKN80_01310"
     CDS             complement(2455..3132)
                     /locus_tag="PKN80_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HNQ34686.1"
                     /translation="MKALDLETFFRERSVSVWGCADLALLRARRTEIASLPGSFRTAV
                     VYGLPLAGGVLDRLEGGPDPLYLHHYRQANYLLDRVGFQLALALEAAGFVSLPVPASQ
                     VIDWERPRGFVSHRHLAAAAGLGWFGRNNLLVTPGWGSRLRLASVLTAAVFPGRPAPR
                     AEAGCGDCRECAGRCPAGAIGATAADFDAEACYRYIKQVCRERNIGQHICGLCLCPFG
                     DGSNPAA"
     gene            complement(3192..4454)
                     /locus_tag="PKN80_01315"
     CDS             complement(3192..4454)
                     /locus_tag="PKN80_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNQ34687.1"
                     /translation="MEKIRALSELELKDRLLKGLESEGLLGRRVLAIIPDSTRTAPIP
                     LAFRTLAEAFRKQGGRLDFLLALGTHPPMTEAEIEAHLKLPASERKRLFPDTEVFNHH
                     WKDPQVLAEIGRIEAGEMASLTGGLYTEAVPVQINRRVFDYDRLLIIGPVFPHEVIGF
                     SGGNKYLFPGISGAEIINAFHWLGALITNPKIIGTADTPVKTVVNRATAFLDRPVSLF
                     AFVMSGHDAVGLFTGGVDDAWRAAVELSRRVNIVYKPRPFRTIFSHMPAMYHDIWLAG
                     KGMYKVEPVAADGGEVIIYAPHIKTVSHTHGEVLERIGYHTRDYFLAQAEKFADVPGG
                     IRAHSTHVKGIGRYVDGIEKPRVTVTLATSIPESVCRRINLNYRDPASLNPEDFKNRE
                     EEGILYVARAGEVLYRLEDGSVPDIDRL"
     gene            4695..6425
                     /gene="dnaG"
                     /locus_tag="PKN80_01320"
     CDS             4695..6425
                     /gene="dnaG"
                     /locus_tag="PKN80_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNQ34688.1"
                     /translation="MGYRITAEILQRIKDENDIVTVVSGYLTLKKSGRSYKAICPFHG
                     EKTPSFIVTPEKGIFHCFGCGAGGDSISFVMRMENLLFPEAAALLAKRAGIQLELTRE
                     DGEANRVRTRLLQLNNEAAAFYHRCLTREKEGQPGRDYLQKRKITAATVKEFNLGFAP
                     SGYPLERHLTAAGFSRAEIEAAGLKFFLRVIFPIYNLSSECLGFGGRTVTDAEPKYLN
                     SPETPVFSKGAGLYGLSLSRQPIQQKGELLLVEGYLDLIKVYQHGFRNVAAGLGTALT
                     PTQARLIRRFTDRVVLLYDGDRAGRLAARRNLEVLLANDLEISVVTLPDQADPDSYLD
                     QHGPEALNKILAGDRKNPLLFQLEMAALEQDLSRPEARINAAREILPLCQVIESPLRR
                     SEYMSQLAEALQLHEEALRQEFHNLQKKSEGRRVETPAVPAPKRKPGLHQAEEILLAA
                     ALCARELRLELARRLTEDHFSDPEHRAVFRKLVQLPETATAEMARAELEGTVQNTEEF
                     LVRVGNLSESEELLEQNFQGCLNRILAHSREERLTRPLRTEKIDGVTDPDLEDLRRFQ
                     ALIQERHQHK"
     gene            6489..7655
                     /locus_tag="PKN80_01325"
     CDS             6489..7655
                     /locus_tag="PKN80_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNQ34689.1"
                     /translation="MTFKNRNDAKQKRINEIIEKGKRQKHLSYDEINEMLPEDSYNPE
                     ELEELFGQLNQLQVAIEDETEEVFTIHEEELEEERLALEEVRNPLRSYLKEAGEISLL
                     THDQEIEIARNIENAKEALQQARARSAGNAELARLERKLDVLKAQLIQANLRLVINIA
                     KKYSNPKLTLRDLIQEGNIGLMKAVDKFRFREGYKFSTYATWWIRQAITRAIADHSST
                     IRIPVHMREKINKLNKISRYLVHELDREPTPEEIARRTQSSPDKVRMILKSMQREPIS
                     LETPVGDDDKTTFGDFIEDRETDTPATAATYALLQDELEKIFAQLDEREEKILRLRFG
                     IGAGYPRTLEEVGQIFNLTRERIRQIESKALEKLRNSEQLKHLSAFLEWYRNSN"
     gene            7897..8637
                     /locus_tag="PKN80_01330"
     CDS             7897..8637
                     /locus_tag="PKN80_01330"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNQ34690.1"
                     /translation="MLLAIDLGNTTLKAVLFDRDGTAAWRVNLRHHNDGARLEARIAK
                     AAWPTGITAAALASVVPDLSGPAAAGLARALRVRPELLDRSRLPLAARVTRPESVGVD
                     RLLNALAAGRLHGKPAVVIDAGTALTLDLVDPDGAYAGGVIAPGPELARDSLAERTAL
                     LPRIELKAPASVVGKDTESCLQAGLVLGFAELVNGLVERLRREWRPEFKLVGTGGSLK
                     ILLPHLRPRPLVDPDLTLRGIWLALQKK"
     gene            complement(8677..10944)
                     /locus_tag="PKN80_01335"
     CDS             complement(8677..10944)
                     /locus_tag="PKN80_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34691.1"
                     /translation="MKATVSGRDYLECLEAWVPEFRRGLYALPDRPGRSCYGPGASNN
                     WAVQTNLKAFAAAAVMAADPAFDETRAGVSRRSMLEHALALLRFSLESHCATGGRCLD
                     GAPWGHTWISMLGIERMAHGVEAIETHLEPGDRDLLRRVLISECDWLLDGYYRGRAER
                     PGEIRAGTVENNHPENNIWAGALLHRAALLYPDAVRAAEYRDKGSRFLVNGISVAADA
                     LSGAVVDGRPVSEWFVGANFFESYALNHHGYLNVGYMVICLSNLAMLHFSCRRAGQEA
                     PGALYHHAADLWNLVKLCTFEDGRLLRIGGDTRVRYCYCQDYAIPAWLMALDCFGDRD
                     CPGFEAGWLAQVRREMANNGDGAFLSDRCRELAGVSPLYFTRLESDRAAALSMGAFWR
                     RTLELPGTAAEQPSPIFLTGAWSDEYHGACLVRGAARTASWTWLAGERPNGLCLPSGD
                     SALAEWRHNLAGEIRGLGRVNYQDLVDHRQAIFDGGFLTWGRTVFRSEKMMCESHPDE
                     ETAGQEIVCAALPDDATMLVLQRARVLARVDLASVKGIFLQVPNDLFNGYRRVYRSGG
                     RVLETNGRDWRAETIRVSGGRLEIDGRLLLEGAYGLDALFIHRPGRRQVGLSVRQDRA
                     DAGGMLLVDEICAPCQLGRRNYDAGAVIYDHGFAISVRPAGAGRVGRWGARPEGLAAP
                     GCPEVRLVAADGRDGVRYLLAANLGGRPAAPEIKAGEARRLSDLAGGGTVDAAGGVFR
                     PELPARTCRLFRLEK"
     gene            complement(11276..12085)
                     /locus_tag="PKN80_01340"
     CDS             complement(11276..12085)
                     /locus_tag="PKN80_01340"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNQ34692.1"
                     /translation="MKSLESAVKVLGSRAALRPEIGIILGTGLSSLAEKIEVETSIPY
                     GEIPGFPVSTVEGHAGNLVLGRISGRPVAVFQGRFHLYEGYSPQQIAFPIRALKAFGV
                     NVLLESNAAGGLNPAYQVGDLVLITDHINLQGFNPLVGPNDENIGPRFPDMSEPYDRL
                     LLDLARQTALREGIQLREGVYVGVLGPNLETRAEYRFLRMIGADLVGMSTVPEVIAAV
                     HAGMRVFGVSVVTDRCLPDALKPVTFDEILAAAGKAEPSLTRLFLKLIESI"
     gene            complement(12122..12916)
                     /gene="proC"
                     /locus_tag="PKN80_01345"
     CDS             complement(12122..12916)
                     /gene="proC"
                     /locus_tag="PKN80_01345"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNQ34693.1"
                     /translation="MELGIIGVGRMGSAIAAGLIERRIFSPGELLVFDLDRERARAFS
                     AAYGPAAAASAADLVRGCRSVLLAVKPQDRSAALEPIGAIGIEGPLISILAGVPTSAL
                     ESIRPGLRVIRVMPNLGVRVGAGLSFYCAGRNGRESDRVLAARIFGGLGEAFEIDESL
                     MNAVTALSGSGPAYFFRLMEIMAGFGRAAGLAPEVAAKLASRTALAAALLARDGDPAA
                     LRGEVTSRGGTTEAAFRVLEEGNLSGLFESALAAAVKRGRELAEGR"
     gene            complement(12970..13701)
                     /locus_tag="PKN80_01350"
     CDS             complement(12970..13701)
                     /locus_tag="PKN80_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ34694.1"
                     /translation="MPKEVVVRTIGVKKNFMLGRIELKVLRGVDLEVSRGEYISIMGP
                     SGSGKTTFFNMIGGLDKPSAGRVYIDEVDIAQLDAYELAWLRCRKIGYIFQTFNLIPV
                     MTALENITLPMIFAGVGHDEAVDRGLKLLEMVGLKERVQHKPFELSGGQQQRVAIARA
                     FANSPAIILADEPTGNLDLKTGKEIIRLLKEMNRNHQVSVITATHDLKMLDVSDRVVW
                     IRDGQIERIEERANLDIKVGEVEGE"
     gene            complement(13714..14349)
                     /locus_tag="PKN80_01355"
     CDS             complement(13714..14349)
                     /locus_tag="PKN80_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNQ34695.1"
                     /translation="MAGQVGKQVMLPKQEAFKMSLRNIRIRFGRACIVSSSVLLGVAF
                     LMSIFTNTVIQQALLKNGPESIKVSLAAGASDSMARDIWLVSLSLLVCVVGITNAMLM
                     SVTERSREIGTMKCLGALNRFIMEIFLIESGMQGLLGSLAGGLIGGLGMLITYLARYG
                     TVILNYIPYLGLLKFYLFSIILGTVISIIGAVYPTYLSARMEPAEAIRREV"
     gene            complement(14330..14677)
                     /locus_tag="PKN80_01360"
     CDS             complement(14330..14677)
                     /locus_tag="PKN80_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34696.1"
                     /translation="MGAQVVVNSAVRVEERGRELLIAIPGRKNFWLNLVSFVVTLPRE
                     KRVFLDELGALIYQDCRQGLTVARIVENFSGRHGLSIDYSRQSVMLYLKMLAEKGVVG
                     FLIKEQTDGGTSR"
BASE COUNT         2895 a         4902 c         4709 g         2338 t
ORIGIN      
        1 gtggaactgt tgatgccgat cggaccgggg gtcatctcgg ccaccgagac cagccgggag
       61 aattccccgt cggtcagcca gcggtgattg accaccagct cccgctggat gagcggcagc
      121 accgcgtaac cgccgccgaa ggcgaagagg ccgatcttca gaaaggtcag gatcagctgc
      181 cagtagatca ccgcccgcct cccgaagcca gtttcaactt cagcccgatc aaaaggagcg
      241 ccgccaggat gaccaggatg ggattgacgc ccaggccgat cagcaggccg agggcggcca
      301 gcgccgccag gatgctctcc gggcggcgca ggtgacgcca ggccagctgg agaaccaccc
      361 cgccgatgat cccgaccacc gccggcaccg caccccggaa gaaggccagc accgccgggt
      421 tgcccatgaa gcggctcagc accgaggcga tcagcaggat ggaaaggaag gagggcagga
      481 tcgccccgga aacggccagc agggcgaccc ggaggccgcc cagttccagg ccgatcaggg
      541 tggacagatt gaccatcatc acccccggcg ttccctgggc gatagtcagg agctccagga
      601 accgttcctc gctgatccaa cccttgcggc ggaccaccat ctccctgacc agcggcagca
      661 tcgcgtagcc gccgccgatg gtgaagccgc cgattttgaa gaaggccaga aaaaaatgcc
      721 agccgttgag ccgtatcctg ttttccattc gggaagccgg ccgggccgaa ccgcccggcg
      781 gcgggaagag gccgcggccg gaggttcaga atcggaaaag acaatcaacc gcccgttcaa
      841 ccggtaaagt aattatacat taaagaaagg ggagcggcaa aacggccggc cgccttgaca
      901 ctccgcgcgg gccggcataa aatagcggtg ccatgaccga ggcccgtttt tacgactccc
      961 tggcaggcgg ccgggtggaa tgtcggctct gcccgcatca ttgccggatc gccccggacc
     1021 agacgggtcg gtgcggagtc cggcggaacc aggcgggcaa actctactcg ctggtatacg
     1081 gacggccggt cgccgaacgg gccgacccga tcgaaaaaaa gccgctcttc cacttcctgc
     1141 ccggctcgac ctccttctcg gtggccaccg ccggctgcaa cctgacctgc cggttctgcc
     1201 agaacagcga catctcccag gtaaacggcc gcataattta cgaacgcgaa ctggcgccgg
     1261 ccgagatcgt cgaacgggcc agggcggccg gctgccgctc cgtctcctac acctacaccg
     1321 aaccgaccgt cttcttcgag tacgtcttcg acaccgcccg gctggccgcc ggggccggac
     1381 tgcggaacgt gctggtcacc aacggttatc tcgaggccga cccgctgcgg gaactcgcgc
     1441 cctacctgga cgcggccaac gtcgacctga agagtttccg ggacgagtac taccggaact
     1501 tctgcggcgg ccggctgcga ccggtcctgg aaacgctgga actgatgagg cggctgggaa
     1561 tctgggtgga ggtgaccacg ctcctggtgc ccggggagaa cgacggcgcc ggcgaactgc
     1621 gggaagccgc cgatttcctg agcggccggc tcggcccgga caccccctgg cacctgagcc
     1681 gcttcttccc ccactaccgg tttatgaacg ctccgcccac gccgccggag accctggccg
     1741 cggcccggga aatcggccgg gcggccggtc tccactacgt ctacaccggc aacctgcccg
     1801 gcgatccggg cgaaaacacc gcctgcccgc aatgcggccg ccaggtgatc ggccggaccg
     1861 gcttcctggt caccgggtac gccctaaccg ccggccgctg cgccgcctgc ggccggtcca
     1921 tcgccggcgt ctttgactga ggccgccctc cgcccgaccc gcagcctttc gagcagaaac
     1981 ggccgcgcct ggccgaaaaa ccgccggtag tactccgacc ggtagtcggg ataggtccag
     2041 ggcagcgcct gaaactcccc gtcccggtaa acgagcgtca cctcgccgta gattccccgc
     2101 cccaggtaga tccggtggct gtaatccttg gtggaggcca ggaccagccg ggccgggtag
     2161 agacagcccg ggtcaatatt aacccggcgg cggccggcct ggctccacgc ctcctccaac
     2221 cggttggtga agactttggc cgcggccagc cggccgggcg gaaaaggacg ccggaagagg
     2281 agcagctgcc gcttcagtcc cgcccccagt tcgcgttcgt aatagtcgct gcagcggaaa
     2341 tcgagcagcc ggctctcggt caacagcgga ccgaagcggc gggtgagttg cgatttcagg
     2401 gaaggaaaga gagccggatc ggcccccagc aggcccacga agaggagtac cggttcaggc
     2461 ggccggattc gacccatcgc cgaaggggca gaggcagagt ccgcagatat gctggccgat
     2521 gttgcgttcc cggcagacct gcttgatgta acggtaacag gcctcagcgt caaaatcagc
     2581 cgccgtcgcc ccgatggctc cggccgggca gcggcccgcg cattcgcggc aatcgccgca
     2641 gccggcctcc gcccgcggcg ccggccggcc cgggaaaacg gccgcggtga gcaccgaggc
     2701 caggcgcaac cggctgcccc aacccggggt aaccagaagg ttgttccggc cgaaccagcc
     2761 gagaccggcc gcggcggcca ggtggcggtg cgaaacgaaa ccacgcggcc gttcccagtc
     2821 gatcacctgg ctggccggaa ccggcaggga aacgaagccg gcggcctcca acgccagggc
     2881 cagctggaac ccgacccggt ccagcaggta attggcctgg cggtaatggt ggaggtaaag
     2941 cggatcgggg ccgccctcga ggcggtccag cacgccgccg gccaggggca gcccgtaaac
     3001 cacggccgtc cggaatgaac cgggcaggga ggctatttcg gttcgccggg cccgcaggag
     3061 ggcgaggtcg gcacaacccc agaccgacac cgaccgctcc cggaagaagg tttccaggtc
     3121 cagggctttc atccctccgt tcccgctcac cgtgtccttc ctttcgccca aagaccgccg
     3181 ccggacctcg gtcaaagccg gtcgatgtcc ggaacgctgc cgtcctcaag ccggtagaga
     3241 acttcaccgg cccgcgccac gtaaaggatt ccctcctcct cccggttctt gaaatcctcc
     3301 gggttgagcg aggccgggtc gcggtaattc aggttgatcc gccggcagac cgattcgggg
     3361 atggacgtcg ccagcgtcac cgtgacccgc ggtttttcga ttccgtccac ataacggccg
     3421 atgcccttga cgtgagtgga atgggcccgg atgccgccgg gcacgtcggc aaacttctcc
     3481 gcctgggcca ggaaataatc ccgggtatgg taaccgatgc gttccaggac ctcgccgtgg
     3541 gtgtgggaaa cggtcttgat gtgcggcgcg tagatgatga cctcgccgcc gtcggcggcc
     3601 accggctcca ccttgtacat gcccttgccg gccagccaga tgtcgtggta catggccggc
     3661 atgtgcgaaa agatggtccg gaacggccgg ggtttgtaaa cgatgttgac ccggcgggaa
     3721 agctccaccg ccgcccgcca ggcgtcatcc actccgccgg taaagagacc gaccgcatcg
     3781 tggccgctca tgacgaaggc gaagagggaa accggacggt ccaggaaagc ggtggcccgg
     3841 ttgacgaccg tcttgaccgg ggtgtcggcg gtgccgatga tcttggggtt ggtgatcagg
     3901 gcgcccagcc agtggaaggc attaatgatc tcggcccccg agatgcccgg gaagagatac
     3961 ttgttgcccc cggagaaacc gatcacctcg tgcgggaaga ccggcccgat gatgagcagc
     4021 cggtcgtaat cgaagacgcg acggttgatc tgcaccggga ccgcctcggt gtaaaggcca
     4081 ccggtaagag aggccatctc gccggcctcg attcggccga tctcggccag tacctgcggg
     4141 tccttccagt gatggttgaa gacttccgta tccgggaaaa gccgcttccg ctcggacgcc
     4201 ggcagcttca ggtgcgcttc gatttcggcc tcggtcatcg ggggatgggt gccgagcgcc
     4261 agcaggaaat ccagccggcc gccctgcttc cggaaggcct cggccagggt gcggaaggcc
     4321 agcgggatgg gcgcggtacg ggtactgtcg gggatgatgg ccaggacccg gcggcccagc
     4381 agcccctcgc tttccagtcc cttcagcagc cgatccttca actcaagctc actcaacgcg
     4441 cggatctttt ccatgtcgga ttccaacttt cgttgtgggc ggataacccg ggcccgaccg
     4501 ccgtgattgt aacaaaaatc cggcccccgc atctaactga tacggggtgc gagcacgcgg
     4561 tttgcaggtt attttggata attttatcac aatccacttg acatttgcca accgtcccgg
     4621 tataaaatta ctaattaaat tttgttgccg ctctccgttc cgttcaccag cctaaattca
     4681 tagaagaaac gaagatgggt tatcgcatca ccgccgaaat actgcagcgc atcaaggacg
     4741 aaaatgacat cgtgaccgtc gtttcgggct acctgacgct caagaagtcg ggtcgttcct
     4801 acaaagcgat ctgtccgttc cacggcgaaa agaccccctc cttcatcgtc accccggaga
     4861 agggcatctt ccattgcttc ggctgcggcg ccggcggcga ttccatctcc ttcgtgatgc
     4921 gcatggaaaa cctcctcttc ccggaggcgg ccgccctgct ggccaagcgg gccggaatcc
     4981 agctcgaact gaccagggaa gacggcgagg ccaaccgggt ccgaacccgg ttgctgcagt
     5041 tgaataatga agcggccgct ttttatcacc gctgcctgac cagggaaaag gaggggcagc
     5101 cgggacgcga ctacctgcag aaaaggaaaa taaccgcggc cacggtcaag gaattcaacc
     5161 tgggcttcgc gccgtccggt tatccgctcg aacgccacct gacggcggcc ggtttcagcc
     5221 gggccgaaat cgaggcggcc ggcctgaaat tcttcctgcg ggtcatcttt cccatttaca
     5281 atctttcaag cgaatgcctc ggtttcggcg gccggaccgt gaccgacgcc gagcccaagt
     5341 acctgaattc acccgaaacc ccggtcttca gcaagggcgc cggcctttac ggcctcagcc
     5401 tgagccggca gccgatccag cagaagggcg aactgctgct ggtcgaaggc tacctggacc
     5461 tgatcaaggt gtaccagcac ggcttccgca acgtggcggc cggcctgggc acggcgctga
     5521 cgcccaccca ggcccgcctg atccgacgct tcaccgaccg ggtggtgctt ctttacgacg
     5581 gggaccgggc cggccggctg gccgcccggc gcaacctgga ggtgctgctg gccaacgacc
     5641 tggagatcag cgtggtgacg ctcccggatc aggccgaccc ggactcctat cttgaccagc
     5701 acggaccgga agccctgaac aagatattgg ccggcgaccg caagaacccg cttctttttc
     5761 aactggaaat ggcggccctc gaacaggatc tgtcccggcc cgaggcccgc ataaacgcgg
     5821 cgcgggaaat cctcccgctc tgccaggtga tcgaaagccc gttgcgccgt tccgaataca
     5881 tgagccagct ggccgaggcg ctccagctgc acgaagaggc gctgcgccag gaattccaca
     5941 acctgcagaa gaagtccgaa ggccgccggg tagaaacgcc ggcggttccg gcgccgaagc
     6001 gcaaaccggg gctgcaccag gccgaggaaa tcctgctggc cgccgccctc tgcgcccggg
     6061 aactgcggct cgagctggcc cggcggctga ccgaggatca tttcagcgat ccggaacacc
     6121 gggcggtctt ccgcaaactg gtccagcttc cggaaacggc cacggcggaa atggcccggg
     6181 ccgaactgga ggggacggtg caaaataccg aggagttcct ggtccgggtc ggcaatctga
     6241 gcgaatcgga ggaattgctg gaacaaaatt tccagggttg tcttaaccgg atcctggccc
     6301 acagccggga ggaacggctt acccggccgc tgcggaccga aaagatcgac ggcgtcaccg
     6361 atccggatct tgaggattta aggcgttttc aggcgctgat ccaggaacgc caccagcata
     6421 aataaggctt cgtcccgggc cggaatcggc ccggttgagg agcggttttg agaatcggag
     6481 gccccttaat gacgttcaag aacagaaacg acgccaagca gaaacggatc aatgaaatca
     6541 tcgaaaaggg caagcggcag aagcaccttt cctatgacga gatcaacgaa atgctcccgg
     6601 aggacagcta taacccggag gaactcgaag agctcttcgg ccagctgaac cagctgcagg
     6661 tggccatcga ggatgaaacc gaggaggtct tcaccatcca cgaggaggaa ctggaggagg
     6721 aaaggctggc cctggaggag gtgcgcaatc cgctgcgttc ctaccttaag gaggccggcg
     6781 aaatctccct gctcacccac gaccaggaga tcgagatcgc ccggaacatc gaaaacgcca
     6841 aggaggccct ccagcaggcc agggcgcgtt ccgccggcaa cgccgaactg gcccgcctgg
     6901 aacgtaagct ggacgtgctg aaggcccagt tgatccaggc caacctgcgg ctggtcatca
     6961 acatcgccaa aaaatactcc aacccgaagc tgaccctgcg cgacctgatc caggagggca
     7021 acatcgggct gatgaaggcg gtcgacaagt ttcgcttccg cgaaggctac aagttttcga
     7081 cctatgccac ctggtggatc cgccaggcca ttacccgggc catcgccgac cactcctcga
     7141 ccatccgcat cccggtccac atgagggaga agatcaacaa gctcaacaag atttcgcgct
     7201 acctggtcca cgaactggac cgggaaccga ccccggagga gatcgcccgc cgcacccaga
     7261 gttccccgga caaggtccgg atgatcctca agagcatgca gcgggagccg atttcgctgg
     7321 agactccggt gggagacgac gacaagacga ccttcggaga tttcatcgag gaccgcgaga
     7381 ccgacacgcc ggccactgcg gccacctacg ccctgctcca ggacgagctg gaaaaaatct
     7441 tcgcccagct ggacgaacgg gaagagaaaa tactgaggct tcgtttcggc atcggcgccg
     7501 gctacccgcg caccctcgaa gaggtaggcc agatattcaa cctcacccgg gaacgcatac
     7561 gccagatcga aagcaaggca ctggagaagt tgcgcaactc ggaacagctc aaacaccttt
     7621 cggcgttctt ggagtggtat cgcaattcca actgatagtg gtctgccttc ggaccggata
     7681 gctttgttgg cggcgtcggc gccggcgtcg gcgtacatcc agtacgcctc ctggacgcct
     7741 cctggccgtc gcgctcccgg cccgaattca aaccactatt gctcaggaat ttgagtcctg
     7801 acagcggtct gccttcggac cggatagctt tgttggcggc gtaccttcaa cgctttttct
     7861 tcggaccgga tttctttcat caaaccaaac cgaaaaatgc tgctggccat tgacctcggc
     7921 aacacgaccc tcaaggcggt cctcttcgac cgggacggca ccgccgcctg gcgcgttaac
     7981 ctgcgccatc ataatgacgg cgcccggctg gaggcccgga tcgcgaaggc cgcctggccg
     8041 accggcatca ccgccgcggc gctggcctcg gtcgtcccgg acctctccgg tccggccgcc
     8101 gccggcctgg cccgggcgct ccgggtgcgg cccgaactcc tggatagaag ccggctgccg
     8161 ctggccgcgc gggtaacccg gcccgaaagc gtgggcgtcg accggctgct caacgcgctg
     8221 gccgccggcc ggctgcacgg aaaaccggcc gtggtaatcg acgccgggac cgccctcacc
     8281 cttgacctgg tcgatcccga cggcgcctat gccggcggcg tcatcgcccc ggggccggag
     8341 ctggcccgcg acagcctggc cgaaaggacc gccctcctgc cgcggatcga attaaaggcg
     8401 ccggcctcgg tggttggaaa ggataccgag tcatgcctgc aggcggggct ggtgctgggt
     8461 ttcgccgaac tggtcaacgg actggtggaa cggctgaggc gggagtggcg cccggaattc
     8521 aagctggtgg gtaccggcgg cagtctgaaa atactgctgc cgcacctccg gccaaggccg
     8581 ctggttgatc ccgacctgac cctccgcggc atctggctgg cccttcagaa aaagtaaccc
     8641 cccacaacgc cgtttttgcc ccttccacgc ggaacgctat ttttccaacc ggaaaaggcg
     8701 gcaggtccgg gcgggcagct ccgggcggaa aacgccgccg gcggcgtcca ccgtcccgcc
     8761 gccggccagg tccgaaaggc gccgcgcctc gccggccttg atttccggcg ccgcgggccg
     8821 gccgccgagg ttggccgcca gcaggtagcg caccccgtca cgcccgtcgg cggcaaccag
     8881 gcgcacctcc gggcagcccg gcgcggccag tccctccggg cgggcgcccc agcgcccgac
     8941 ccggccggcg cccgccggcc ggacgctgat ggcgaaaccg tggtcgtaga tgaccgcccc
     9001 ggcgtcgtaa ttacgccgtc cgagctggca gggggcgcag atctcgtcaa ccaggagcat
     9061 cccgccggca tccgcccggt cctggcgcac cgaaaggccg acctggcgcc ggccaggccg
     9121 gtggatgaaa agggcgtcca ggccgtaagc gccttcaagg agcaggcgcc cgtcaatctc
     9181 gaggcggccg ccggacacgc ggatggtttc ggcgcgccaa tcgcgcccgt tggtctccag
     9241 aacgcgaccg ccagagcggt agacgcggcg gtagccgttg aaaagatcgt tgggaacctg
     9301 gaggaaaatc cccttgaccg aggccaggtc cacccgggcc aaaacccgcg cacgctggag
     9361 caccagcatc gtcgcgtcgt caggcaaggc ggcgcaaacg atttcctgac cggcggtttc
     9421 ctcgtcgggg tggctttcgc acatcatctt ctcagagcgg aagaccgtcc ggccccaggt
     9481 gaggaacccg ccgtcgaaaa tcgcctggcg gtggtcgacc aggtcctggt aattgacccg
     9541 gcccagcccc cggatttccc cggccaggtt gtggcgccac tcggccagcg cgctgtcgcc
     9601 cgagggaagg caaagcccgt tgggacgctc gccggccaac caggtccagg aggcggtgcg
     9661 cgcggcgccg cgcaccaggc aggcgccatg gtactcgtcg ctccaggcgc cggtgagaaa
     9721 aatcggcgac ggttgttccg cggccgtgcc gggcagttcc agcgtccgac gccagaaggc
     9781 ccccatggac agagcggcgg cccgatccga ttccagccgc gtgaaataaa ggggcgagac
     9841 cccggccagt tcccggcagc ggtccgaaag gaaggcgccg tcgccgttgt tggccatctc
     9901 gcggcgcacc tgagcaagcc agccggcctc aaaaccgggg cagtcgcggt cgccgaagca
     9961 gtccagcgcc atcagccagg cggggatcgc gtaatcctgg cagtagcagt agcgcacccg
    10021 cgtgtcgccg ccgatacgca gaaggcggcc gtcctcgaag gtgcagagct tgacgagatt
    10081 ccagaggtca gccgcgtggt ggtaaagggc cccgggtgcc tcttggccgg cgcgccgaca
    10141 cgagaagtgg agcatggcca ggttggagag gcagatgacc atgtaaccga cgttaaggta
    10201 gccgtggtgg ttgagcgcgt acgactcgaa gaaattggcg ccgacgaacc actcggaaac
    10261 cggtcggccg tcgaccaccg ccccggaaag cgcatcggcg gcaaccgaga tgccgttgac
    10321 caggaaacgc gaacccttgt cccggtattc ggcggcgcgc acggcgtccg ggtagagcag
    10381 ggccgcccga tgcaggagcg ccccggccca gatgttgttc tccgggtggt tgttttccac
    10441 ggtgccggcc ctgatttccc cggggcgttc ggcgcggccg cggtagtagc cgtccaacag
    10501 ccaatcgcac tcggagatca ggacccgccg caggaggtcg cggtctcccg gttccaggtg
    10561 cgtctcgatg gcctcgacac cgtgcgccat gcgctcgatg cccagcatcg agatccaggt
    10621 gtggccccag ggggctccgt caaggcagcg gccgcctgtg gcgcagtgac tctccaacga
    10681 gaagcgcagc agcgcgaggg cgtgctcgag catggaccgc cgggaaacgc cggcccgcgt
    10741 ctcgtcgaag gcggggtcgg cggccatgac cgcggcggcc gcgaacgcct tgaggttcgt
    10801 ctggacggcc cagttgttcg aggcccccgg cccgtagcag gagcgtcccg gacggtccgg
    10861 gagcgcgtag agcccgcgcc ggaattccgg cacccaagcc tcgaggcatt cgaggtagtc
    10921 gcgtccggag accgtcgctt tcatctcccc tcccccgggc ggcattgccg gcggcgacca
    10981 ggcagtgcca cgacccggaa atcagttttc gtaaaaaacc cgatacgccc ggtagacgcg
    11041 gtaaaaatcg gccttgcggc agacctcccg gggcgaaggc atcaagagca ggaccggccc
    11101 cacgtccacc gtttccgtgt tgaaccggac cagatacttg gcgaccgtgt acccacaacc
    11161 ggttttttcg cgacccttga agaactgact gattcggtgt gcacccagtt cggagaatgg
    11221 aaacgtggaa atgcgggact tgtcaggtta tgcgcaatca atcaagacgc tgtgtctaaa
    11281 ttgactcgat cagcttcagg aagaggcggg tcagcgacgg ctcggccttg ccggcggccg
    11341 ccagtatctc gtcgaaggtg accggtttga gcgcgtccgg caggcagcgg tcggtcacca
    11401 ccgacacccc gaaaacccgc atcccggcgt gaaccgcggc gatcacttcc ggcaccgtgg
    11461 acattccgac caggtcagcc ccgatcatgc gcaggaagcg gtactccgcc cgggtctcca
    11521 ggttggggcc cagcacgccc acgtaaaccc cttccctgag ctggattccc tcccggagcg
    11581 cggtctggcg ggccaggtcc agcagcagcc ggtcgtaagg ttcggacatg tccgggaagc
    11641 gggggccgat attctcatcg ttggggccaa ccagggggtt aaacccctga agattgatat
    11701 gatcggtgat caggaccagg tcgcccacct ggtaggccgg gttcaggccg ccggccgcgt
    11761 tggactcgag gagaacattg actccgaagg ccttcagcgc gcgaatcgga aaggcgatct
    11821 gctgggggga gtatccctcg taaagatgga accgaccctg gaagaccgcc accggccggc
    11881 cgctgatccg gcccaggacc aggttgccgg catgtccctc gaccgtactg accgggaagc
    11941 ccggaatttc tccgtagggt atcgaggtct cgacctcgat cttttcggcc agggaggaga
    12001 gcccggtccc caggatgatg ccgatttccg gccggagcgc ggcgcgcgac cccaggacct
    12061 tgaccgccga ttccagagat ttcattattt cgctcatcgt tcggatctcc aattaacccg
    12121 gttacctgcc ttcggccagt tcccggccgc gctttaccgc ggccgccagg gccgattcga
    12181 acaacccgga aaggttccct tcctccagca cccggaaagc cgcctcggtg gtgccgccgc
    12241 gcgaggttac ctccccgcgc agggcggccg ggtcgccgtc gcgcgccagc agtgcggccg
    12301 ccagggcggt ccggctggca agtttggcgg cgacctccgg cgccaggccg gccgcccggc
    12361 cgaacccggc cattatctcc atgagccgga agaagtaagc cgggccggaa ccggaaaggg
    12421 cggttaccgc gttcatgagg gattcgtcga tctcgaaggc ctcgcccagc ccgccgaaga
    12481 tgcgggcggc cagaacccgg tccgactccc gtccgttccg gcccgcgcag taaaaactga
    12541 ggccggcccc gacccggacg ccgaggttgg gcattacccg gatgacgcgg agaccgggac
    12601 ggatggattc aagggcggaa gtgggaacac cggcgaggat ggaaataaga gggccttcga
    12661 ttccgatcgc cccgatcggt tccagggccg ccgaacgatc ctgcggcttg accgccagca
    12721 gcaccgaacg gcagccccgc accagatccg ccgccgaggc ggccgcggcc ggtccgtaag
    12781 ccgccgagaa ggcgcgggcg cgttcccggt ccaggtcaaa aaccagcagc tcgcccgggc
    12841 tgaaaatccg gcgttcaatc aggccggcgg cgatggccga acccatccgg cccacgccga
    12901 taattcccag ttccatcgat tccgaagtgg gcatcagaaa aaaggcgcgc ggccggtggc
    12961 tcagccgccc tactcgcctt cgacctcgcc gaccttgatg tccaggttgg cgcgctcctc
    13021 gatgcgctcg atctgtccgt cccggatcca gaccacccgg tcggagacgt cgagcatctt
    13081 caggtcatgg gtggcggtga taaccgaaac ctggtggttc cggttcatct ccttgagaag
    13141 gcggatgatc tccttgccgg tcttcaggtc gaggttgccg gtcggttcgt cggccaggat
    13201 aatggccggc gagttggcaa aggcccgggc aatggcgacc cgctgctgct ggccgccgga
    13261 aagttcgaag ggtttgtgct ggacccgttc cttgaggccg accatctcca gcaatttcaa
    13321 gccgcggtcc accgcctcgt cgtgaccgac cccggcgaag atcatcggca gggtgatatt
    13381 ctcgagggcg gtcatgaccg gaatcaggtt gaaggtctga aagatgtacc cgatcttccg
    13441 gcagcgcagc caggccagtt cgtaagcgtc cagctgggcg atgtcgacct cgtcgatgta
    13501 gacccggccg gccgaaggct tgtccaggcc gccgatcatg ttgaaaaagg tggtcttgcc
    13561 cgacccggac ggtcccatga tcgagatata ctcaccgcgg gagacctcca gatcaacacc
    13621 ccgcagtacc ttcaactcga tgcggcccag catgaagttc ttcttaaccc cgatggtccg
    13681 gacgactact tccttgggca tcgttattcc tccttaaact tcccggcgga tggcctcggc
    13741 cggctccatc cgggccgaaa gataggtcgg gtaaacggcc ccgatgatgg agatgaccgt
    13801 tcccaggatg attgaaaaca ggtagaattt cagaagaccc aggtagggaa tgtagttgag
    13861 aatcaccgtc ccgtagcggg ccaggtaggt aatcagcatg cccagcccgc cgatcagccc
    13921 gccggccagc gaacccagca atccctgcat gcccgactcg atcaggaaga tctccatgat
    13981 gaaacggttc agagcgccca ggcacttcat ggtaccgatc tcccggctgc gttcggtcac
    14041 cgacatcagc atggcgttgg taatgcccac cacgcagacc agcagggaaa ggctgaccag
    14101 ccagatgtcc cgggccatcg aatcggaggc gccggccgcc aggctgacct tgatgctctc
    14161 cgggccgttc ttgagcagcg cctgctggat aaccgtgttg gtaaaaatgg acatcaggaa
    14221 ggcgactccg agcaggacgc tggaagaaac gatgcaggcg cggccgaaac ggatcctgat
    14281 gttgcgcaaa ctcatcttga aggcttcctg cttgggaagc attacctgct tacctacttg
    14341 tcccgccatc ggtctgctcc ttgataagaa atccgaccac acccttctcg gccagcatct
    14401 tcaggtagag catcacgctc tgccggctgt aatcgattga aaggccgtgc cgccctgaaa
    14461 aattctcgac gatccgggcg acagtcaacc cctgccggca atcctggtaa atcaaagccc
    14521 ccaactcgtc caggaagacc cgtttctcgc ggggcagggt caccacgaac gaaaccaggt
    14581 tgagccagaa atttttgcga ccggggatgg cgatcaacaa ctcccggccc cgctcctcga
    14641 cccggacggc ggagttgacc accacctgcg cgcccatcat ctgatccggt ccgagcggcc
    14701 gacggcccgg cggctcgccg ccgggcgcgg atccggaagg ccgcggacta gaaacaaccc
    14761 gccgtttgaa gaggcggtcc aaaaaaacag gtgcttcgcc aaggcctggg atccagcgca
    14821 tttttacaat tataaccttt ttcc
//
LOCUS       DAOQYN010000016        14619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_4583_length_14619_cov_4.337888, whole genome shotgun sequence.
ACCESSION   DAOQYN010000016 DAOQYN010000000
VERSION     DAOQYN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14619
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4583_length_14619_cov_4.337888"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PKN80_01365"
     CDS             <1..425
                     /locus_tag="PKN80_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNQ34697.1"
                     /translation="RRGAYPLALRHRLRRRRAGRPCLTTGQADVFGGEIPATPYADNR
                     GDRIGRAILKARRPGCPAVILGGHGLFAFGPDPVAAVKAARLAEYFARLNCFALLLGR
                     SAGRELTGLAAAEVRKWNRRYQGGGYGQEPARRGVSRR"
     gene            446..1507
                     /locus_tag="PKN80_01370"
     CDS             446..1507
                     /locus_tag="PKN80_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ34698.1"
                     /translation="MKQSRDPNTANFLKAVSFERPDWVPAVIDIKLSAWLRHGAALEE
                     IVLAHPVMFSNYRPGDFRRAVSRPRFAPGTRRDGWGIVWDNIEAGLGSIPREAEAPLR
                     DWKDLADYRPPSPDLDSSGGRRDWKALAGSLAARTGRGGLACTTVPHGFIYMWLYYLR
                     GFTNLMLDLGSEEPRLEKLLELVVAHNLELVEKMVGVGTEMIYFGDDLGQQKSLAISP
                     AAWRRWLKPAYRRVLRPCRAAGVLVYFHSDGRIVDIIGDLVETGVDILNPQFRANTLE
                     GLRPFRGRVCLSLDLDRQLFPFAEPARLESHVREAVRVLNRPEGGLLLHAEIAHDVPL
                     ANIEALCRALENYAGPWHS"
     gene            1519..2535
                     /gene="argC"
                     /locus_tag="PKN80_01375"
     CDS             1519..2535
                     /gene="argC"
                     /locus_tag="PKN80_01375"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNQ34699.1"
                     /translation="MKKVRAGIVGGAGYTGGELLRWLLRHPSLEVTLVTSESQAGEPY
                     AAAHPGLPGYEGRRFQAYDPERLARECDFVFLAKPQPNSFAQVRALFGRVRLIDLSGD
                     FRLGSPEKFERWYHEKHGAPEFLKEAVYGLPEFLAPELRAARLVANPGCYATGVLLAL
                     LPLLKAGLVEPEALVSAVSGFSGAGRTPSSTTTAYSVVDNLRPYKVVNHPHQGEILEV
                     LSRLTGHPLDLEFIPQVAGFERGILEVIFCRFQPDLEPASTADVLEKAYRSAPFVRVH
                     PAGRLPELKGVVNTNFCDLGWQPLKGGRLLLVAALDNLVKGAAGQAIQNLNLMAGLPE
                     TAGL"
     gene            2553..3431
                     /gene="aroE"
                     /locus_tag="PKN80_01380"
     CDS             2553..3431
                     /gene="aroE"
                     /locus_tag="PKN80_01380"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNQ34700.1"
                     /translation="MNSGRSGADSRTRLAALFGHPVGHSLSPVFQNAAFRQAGLNAVY
                     LAFDVGPERLPAALAAVRGLDLLGANLTIPHKERAGVCLDELSRLAERTGSVNTVVNR
                     DGRLRGETTDGSGFLRALEETAGVNPAGRRVLLLGTGGAGRALAFALAEAGAELWLVN
                     RTAGRAERLAGELARFHPAGRINVGAFETRAALLERAGIEVLVNATSVGLKPGDPPLF
                     AAGELPGAGLTVCDLIYHRETDLLKAAAARGLKRVDGLGMLVHQGAISFELWTGRPAP
                     LAAMFEALSPGRVKKT"
     gene            3449..4180
                     /gene="radC"
                     /locus_tag="PKN80_01385"
     CDS             3449..4180
                     /gene="radC"
                     /locus_tag="PKN80_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNQ34701.1"
                     /translation="MADNRRPSTAAGKGGRRSGIAAWPSDDRPREKLFRQGEHQLSGA
                     ELLAILIGSGRVGEDALTLARRILARFKTFREMSHTDPRDWLEFKGMGPAKLARIRAA
                     LEIGRRFREVEVRASRRKISAPADIVEAYRDRLRDLKHEVFKVLLLDGRNRIIEDREI
                     VQGTTNGAYPVLREILNEASRRFASGLVAMHNHPSGDPSPSREDREFTARLNEAAELL
                     GLKLLDHVIFGDQPPYFSFTEQDLL"
     gene            4471..5754
                     /locus_tag="PKN80_01390"
     CDS             4471..5754
                     /locus_tag="PKN80_01390"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ34702.1"
                     /translation="MSEKTEVTEPAGPDSAGPLLNGFGRAFSAMRHRNFRLFWIGQAI
                     SSCGGWMQNTVFGWLTLQVTDSAFMLGVVSSISGLPLLLFSLPAGVLADHVSKRRLLL
                     VMQSGLMVSAFVMAVLVTLGLVRFWNIALIAGLTGVLVSFDAPVRQSYVVDLASREDL
                     SNAIALNSANFNTSRIIGPVLAALAIASFGMASVFYINSASFLAAIWALVLIKDTDRP
                     RPSIRRPVRELFQEGLEHVWRERPVRTLLFQAAILSVFGFMGHTLMPVFARDVLNAGV
                     KGLAHLMSSLGIGSLLGAFSGMLFSHHPNRERQVSLGGLVMAAGLFGLACSRYLWLSM
                     GMLLLVGWGAVVQAVTINSLLQQMAPEHLHARVVSVYTMVFSGLMPFGTFLAGTIAQF
                     WGAPAAVIFGASVCLVGSFHYLSLTCRKGRRRPAV"
     gene            5947..6984
                     /gene="aroB"
                     /locus_tag="PKN80_01395"
     CDS             5947..6984
                     /gene="aroB"
                     /locus_tag="PKN80_01395"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNQ34703.1"
                     /translation="MIGGRLDDLAGRLSIFGVGRLFLITDRRVHELYGAKLLRDLSGA
                     GWRTAAAVLPPGERTKSLYRLERLYAAGVEAGLERLSLVLALGGGVVTDLAGFFAATF
                     LRGLPLVNVPTTLLGQVDAAIGGKNGLNLPAGKNLAGTFYQPRLVYCDLDTLATLPFR
                     DYRSGLAEIIKCGVIRDPDLFDLMERGIDGFKRRDPYLLRRAVTAAVRVKRALVEADP
                     LERTGLRRELNFGHTVGHGLEKAGGYRRFRHGEAVALGMLAEAELARRRGLLASAAAD
                     RLGRLVAAYGFRTRAAGCPLEGVLSALLHDKKKQDGRVKFVLPAAIGRTVTCDDVAQA
                     ELAAVLKIFIN"
     gene            7021..8271
                     /locus_tag="PKN80_01400"
     CDS             7021..8271
                     /locus_tag="PKN80_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNQ34704.1"
                     /translation="MKKSRFKWQEEKESDEPPWLYDGSSLMRNGQEEAAARKRAGRRR
                     MTIGLAVLAAVVLFLLLFRFEDVVRAGGEVEPLNILYIKAPLTGTIDQLPFSEGQEIK
                     QGQLLVSIWPEELDIFRDVENKRYEFENANREFQKENERLVMLAVKYERLKREKAIFE
                     GDDSEVRVLQERIQAAGVTLDQRSKDEKRSKNLFQQGVVSAAEYENVLANLELSRSVY
                     AEAGARLHALKNERAIRIEQLTRDIEMTEKELSVSRISSGQWEAEQVQKSSMLEAASK
                     RAELMLITAPSDGQIVRQEKKLGDRVGNGDLIFVLSTGRERRLSVRIDPVDSLRVEVG
                     QPVRVYSSLFSRRYGYASGRVIELGTYARSQSGPGLQTYIPAKVAVDQPPFPLLLGTR
                     VEVQILTGRKRTLLSRRPSGRGGK"
     gene            8268..10301
                     /locus_tag="PKN80_01405"
     CDS             8268..10301
                     /locus_tag="PKN80_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ34705.1"
                     /translation="MNNAPEPAGPVETPIVLGSVDRIPGLILQRLQDTAIQDEEILLA
                     AEADLDELGHIEPVWLLAGTKHVVAVPAVPDRPLRGPYAYAVTNDFDLHPSIGSTSVR
                     VFVEKTPFELVRFTNEFRERFQRVVVQLKRIRNNEPIQPEALLKLNEDFCPKCGLILP
                     RSGAPCRRCVKHGAVFQRMFAILWHYRWLMLALFLCMIAGIVLDMLPPYLTRILVDNV
                     LGSQSHPEWLLWLVASLAGAALLRRGIDVIISQLTPRVGTAMTNDIRKRLFQKLQEMS
                     VAYYDRVQVGSLMSRVINDVEVMHGFVTQVTQGFLVNLLLILFIGVMLFYLNARLAFF
                     VLIPIPFVVIGTLFFWNRIYPNYYKYWESNSKLGAMLNGVLSGIRLVKVFGQEQRETS
                     RFNRSVDYLREARIRVDTRVGAFSPVMAYFFGLGGLIIWFVGGRDVLSGVITLGTLMA
                     FLGYIGMFYAPLGSLAMFSNWVTQFATASQRIFEILDMPSETLDAPDAIDCPDIRGDI
                     EFKDIWFGYDPYNPILKGISLKIQAGEKIGIVGRSGSGKTTLINLLCRFYDVQRGRIL
                     IDGEDIRHFKQTQLLQHLSLVLQEPFLFRGSVRENIAYGRPEATFEEILNASKAANCH
                     DFIMKLSEGYDTPLGERGAGLSGGEKQRISIARALLCNPRILMLDEATSSVDT"
     gene            10534..11109
                     /locus_tag="PKN80_01410"
     CDS             10534..11109
                     /locus_tag="PKN80_01410"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HNQ34706.1"
                     /translation="MEKNRESAVVKKVMDYGGVGIRILKADRVKIYRDIDNLLYAEVA
                     EAGLETPRLYRGVFAVLCFPVSGPDRYISLRYVNPSGQEEEIGVIREPIEFPREDRIQ
                     LAKSLAQYYFEFEIERIVSIEFRYNLLMFDVLTNFGPRQFEMRWRGDRALSLGEKGKV
                     LLDVYDCRYIVRDVSALPREDQALFNRFIYW"
     gene            11169..12242
                     /gene="pheA"
                     /locus_tag="PKN80_01415"
     CDS             11169..12242
                     /gene="pheA"
                     /locus_tag="PKN80_01415"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNQ34707.1"
                     /translation="MDKEIEKRRQAINKLDSQLLKLLSERAARIVEIGRLKKEKGLTF
                     YDPARERRILERLKKENPGPLNSEAVGAIFMEVISSCRFLEYPTRIAYLGPEATFTHQ
                     AALKTFGSQALFLPQKSTSSVFSAVEKSGADYGVVPVENSTEGVVSHTLDMFMDSDLK
                     IVNEVLLPVRHHLLGFGSLKEVREVYSHPQALGQCQRWLEENLVKVTLHETESTAEAA
                     RLVSNRKGAAALASEIAATLYGLPIMVRDVQDHSRNYTRFLVIGRHFSGRTGRDRTSI
                     LFSIKDRVGALHDMLLPFQGQGLNLTKIESRPTKRRAWEYVFFVDFLGYKDDPEVREA
                     LSELEKLCLFLRVLGSYPVADEP"
     gene            12232..12738
                     /locus_tag="PKN80_01420"
     CDS             12232..12738
                     /locus_tag="PKN80_01420"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HNQ34708.1"
                     /translation="MNPEVRLPLLLLLFGHVVADYLCQPRSLTFLKRRRPVFLLLHGL
                     VVLAWLWPLAILYPGRAVLLLLTAVAASHLAIDAVKIGLERRCCFQRREKRLANVIDQ
                     GLHFLALAAAWWLGFRGRLWPAALPRTPVLLNSLLVLVIILIGVKAGFGFLETGREDD
                     HNLTTGHD"
     gene            12707..13720
                     /gene="aroF"
                     /locus_tag="PKN80_01425"
     CDS             12707..13720
                     /gene="aroF"
                     /locus_tag="PKN80_01425"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNQ34709.1"
                     /translation="MIITLRPGTTEEGVREVLEKIKDLGFTPHLSSGKERVVIGVIGE
                     NAILTREQFEAMFIVESVTPISRPYKLVSREFKKENTVITAGGVEFGGSEFVVTAGPC
                     AIESREQILETARAVRAAGARVLRGGAFKPRTSPYAFQGLGEEGLKYLAEAGKELGMP
                     TVTEVMDPRQVELVCRYADIIQIGARNMQNFDLLKEVGRAKRPVLLKRGMSARIEEFL
                     MSAEYILSQGNYQVILCERGVRTFEDATRFTLDLAAVPLVKELSHLPIVVDPSHGTGR
                     RNLIAPMSRAALAAGADGLLIEVHPKPEAAVSDGFQSLRFDDFKKLMASLQTLAPVLE
                     RRI"
     gene            13740..>14619
                     /locus_tag="PKN80_01430"
     CDS             13740..>14619
                     /locus_tag="PKN80_01430"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNQ34710.1"
                     /translation="MSELTVRPVDRLAGEIRLPGDKSISHRALLISALAEGKSIITGL
                     QSGADCLATRRVLAGLGVRIETLADGSVAVFGSGPAGFRASPSILDCANSGTTMRLMA
                     GIAAGLPFATSLTGDASLRKRPMARVTVPLREMGARIQAEPGELPPLIIQGGRLTGRE
                     HRLTVASAQVKSCLLLAGLLAAGRTTVIEPAETRDHTERMLGYFGGRVERAGRTLSIE
                     GGQRLAGRPVRVVGDFSAAAFFLAAGAIVPEAELVIPEVGLNPSRTAFLELLRRMGAV
                     IEAEPGGETAGEPSGRL"
BASE COUNT         2382 a         4805 c         4741 g         2691 t
ORIGIN      
        1 ggcggcgtgg tgcataccca ctcgcccttc gccaccgcct tcgccgccgc cgggctggcc
       61 gtccctgcct gaccaccggc caggccgacg tcttcggtgg cgagattccg gcgacccctt
      121 acgccgacaa ccggggcgat cggatcggcc gggcgattct caaggcccgg cggccgggct
      181 gcccggcggt catcctgggc ggacacggcc tttttgcctt cggcccggac ccggtcgccg
      241 ccgtcaaggc cgcccggctg gcggagtatt tcgcccggtt gaattgtttt gcgctcctgc
      301 tgggccggtc ggccggccgc gagttgaccg gcctggcggc ggccgaagtc cggaagtgga
      361 accggcgtta ccagggcggc gggtacggtc aggaaccggc ccggcgcggg gtcagccggc
      421 gatgacttcc ccggccggaa agcaggtgaa acaatcccgc gaccccaata ccgccaactt
      481 cctgaaggcc gtctcctttg agcggccgga ctgggtgccg gcggtgatcg acatcaagct
      541 ctcggcctgg ctgaggcacg gcgccgccct cgaggaaatc gtgctggccc acccggtgat
      601 gttttccaat taccggccgg gggatttccg ccgggcggtc tcgcgtccgc gtttcgctcc
      661 cggcacccgg cgcgatggct ggggcatcgt ctgggataat atcgaggccg ggctgggctc
      721 gattccgcgc gaggccgaag cgccgctgcg tgactggaag gacctggctg attaccggcc
      781 gccgtcgccg gacctcgatt cgtccggcgg ccggcgtgac tggaaggccc tggccggatc
      841 cctggcggcc cggaccggcc ggggcggcct ggcctgcacc accgtgccgc acgggttcat
      901 ctacatgtgg ctttactatc tgcggggctt caccaacctg atgcttgacc tgggttccga
      961 agagccgcgg cttgaaaagt tgctggagct ggtggttgcc cataacctgg aactggtcga
     1021 aaaaatggtc ggcgtcggca ccgagatgat atacttcggt gacgacctcg gccagcagaa
     1081 gtcgctggcc atcagcccgg ccgcctggcg ccgatggctc aagccggcct accggagggt
     1141 cctgagaccc tgccgggcgg ccggggttct ggtctatttt cactccgacg gccggatcgt
     1201 ggatatcatc ggtgacctgg ttgagaccgg ggtggacata ctcaacccgc agttccgggc
     1261 caacaccctg gagggattgc ggcccttccg cggccgggtc tgcctgagcc tggaccttga
     1321 ccgccagctg ttcccttttg ccgaaccggc ccggctggag tcgcatgtcc gggaggcggt
     1381 ccgcgttctg aaccggcccg agggcggcct gctgctgcac gccgaaatcg cccacgacgt
     1441 gccgctggct aatatcgagg cgctttgccg ggcgctggaa aattacgccg gcccctggca
     1501 cagttgagag aggcaaagat gaagaaagtg cgcgcaggaa ttgtcggcgg cgccggctac
     1561 accggcggcg aactgcttcg ctggctgctc cgccacccct ccctcgaggt gaccctggtg
     1621 acctccgaga gccaggccgg ggaaccttac gcggccgccc acccggggtt gcccgggtac
     1681 gagggtcgcc gtttccaggc ctatgatccg gaacgcctgg cgcgggagtg cgatttcgtc
     1741 ttcctggcca aaccccagcc gaactccttc gcccaggtcc gcgcactctt cggccgggtg
     1801 cggctgatcg atctttcggg cgatttccgc ctgggttccc cggagaagtt cgaacgctgg
     1861 taccacgaaa agcacggcgc cccggaattt cttaaggagg ccgtttacgg cctgccggag
     1921 ttcctggccc cggagctgcg cgcggcccgg ctggtcgcca atcccggctg ctacgccacc
     1981 ggcgttctgc tggccctgct gcccctgctg aaggccggcc tggtcgagcc cgaggcgctg
     2041 gtgagcgccg tctccggttt ctccggggcc ggccgcacgc cttccagtac caccaccgcc
     2101 tactcggtgg tcgacaacct gcgtccttac aaggtggtca accatccgca ccagggcgag
     2161 attctcgagg tgctctcccg cttgaccggg catcccctgg acctggagtt cattccccag
     2221 gtggccggtt tcgagcgcgg catcctggag gtgatcttct gccggttcca gccggacctc
     2281 gaaccggcct cgacggccga cgtactggag aaggcctacc ggtcggcgcc tttcgtgcgc
     2341 gtccatccgg ccggccggct gccggaactg aagggcgtgg tcaacaccaa tttctgcgac
     2401 ctgggctggc agccgctcaa gggcggccgg ctgctgctgg tggcggccct ggataacctc
     2461 gtcaagggcg ccgccggcca ggcgatccag aacctgaacc tgatggccgg ccttcccgag
     2521 accgccggcc tgtgatgacc ggagacgcca ggatgaacag cggaagatcg ggggcggatt
     2581 cccggacccg gctggcggcg cttttcggcc acccggttgg acacagcctc tcgcccgtct
     2641 tccagaacgc ggccttccgc caggcgggcc tgaacgcggt ttacctcgcc ttcgacgtcg
     2701 ggccggaacg gctgcccgcg gccctggccg cggttcgggg cctggacctg ctgggcgcca
     2761 acctgaccat tccgcacaag gaacgggccg gagtctgtct ggacgaactc tcccggctgg
     2821 cggaacgcac cggttcagtc aacacggtcg tgaaccggga cggccggctg cggggagaga
     2881 cgaccgacgg cagcggtttc ctgcgcgccc tggaagagac ggccggagtg aacccggccg
     2941 gccgccgagt gctcctgctg ggaaccggcg gggccgggcg cgccctggcc ttcgccctgg
     3001 ccgaggccgg cgccgaactc tggctggtca accggaccgc cggccgggcg gaacggctgg
     3061 ccggcgaact ggcccgtttc catccggccg gccggatcaa cgtcggtgcc ttcgagacga
     3121 gggcggcctt gctggagcgg gccgggatcg aggtcctggt caacgccacc tcggtcggtc
     3181 tgaaaccggg tgatccgcct ctcttcgcgg ctggtgaact gccgggggcc ggattgacgg
     3241 tctgcgatct catctatcac cgggaaaccg acctgcttaa agcggccgcc gcgcgcggtt
     3301 tgaagcgggt ggatgggctt ggaatgctgg tccaccaggg agcgatttcc ttcgagttgt
     3361 ggaccggccg gccggcgccg ctggcggcga tgtttgaggc cctgtccccc ggaagagtca
     3421 aaaaaacttg aagacagggg gcttcaacat ggccgacaac aggagaccgt caaccgcggc
     3481 cggtaagggc ggccggcgtt ccggcatcgc cgcctggcct tccgacgacc gtccgcgtga
     3541 aaaactcttc cgccagggcg agcaccagtt gagcggcgcc gaactgctgg cgatcctgat
     3601 cggcagcggc cgggtcggcg aagatgcgct gaccctggcc cgccggattc tcgcccgttt
     3661 caaaaccttc cgcgaaatga gccataccga cccgcgcgac tggctcgaat tcaaggggat
     3721 ggggccggcc aagctggccc gcatccgggc cgccctcgag atcggccgcc ggttccggga
     3781 ggttgaggtc cgcgcctccc gccggaagat ctcggccccg gccgatatcg tcgaggcgta
     3841 ccgggaccgg ttgcgcgatt tgaaacacga ggtcttcaag gttctgctgc tggacggccg
     3901 gaaccggatc atcgaggacc gggagatcgt ccagggcacc accaacggcg cctatcccgt
     3961 ccttcgcgag atactgaacg aagccagccg gcgattcgcc tccggactgg tggccatgca
     4021 caaccatccc agcggcgacc cttcgcccag ccgtgaggac cgcgagttca ccgcccgtct
     4081 caacgaggcg gccgaactgc tcgggctgaa gctgctggac cacgtcatct tcggggatca
     4141 gcccccgtac ttcagcttta ccgagcagga cctgctgtaa accgaaccga tcccggcgct
     4201 tccggatcgg gccgccgatc agttccggcc gcggattttt tgccggcggc ggcggccgcc
     4261 ggtttgattt ttgagccgga aagaggtaaa atctcaacgt gggaccagcg atcggcggcc
     4321 gggtttgaag acccggccga tttctttata gccgccgtcc gcccgaaaaa agtccgccgc
     4381 cgatttcgtt cattcaaggc cccgggtgtt tcgaacgccc cgtcttcggc gcccgtcctg
     4441 cttacccagg ttgcgcttgg caacatcgac atgagtgaaa aaacagaggt taccgaaccg
     4501 gccgggccgg actcggccgg gccgttatta aacgggtttg gccgcgcctt ctcggcgatg
     4561 cgccatcgca acttccgcct cttctggatc ggccaggcga tcagttcctg cggcggctgg
     4621 atgcagaaca ccgtcttcgg ctggcttacc ctccaggtga ccgactcggc cttcatgctg
     4681 ggcgtggtca gcagcatcag cgggctgccc ctgcttttat tctccctgcc ggccggggtg
     4741 ctggccgacc atgtcagcaa gcggcgcctg ctgctggtca tgcagagcgg cctgatggtg
     4801 agcgccttcg tgatggcggt tctggtcacc ctgggcctgg tccggttctg gaacatcgcc
     4861 ctgatcgccg ggttgaccgg cgtcctggtc tccttcgacg cgccggtccg ccagtcctac
     4921 gtggttgacc tggcctcccg ggaggacctc tccaacgcca tcgcccttaa ctcggccaac
     4981 ttcaacacct cccggatcat cggaccggtg ctggcggccc tggcgatcgc ctccttcggc
     5041 atggccagcg tcttctacat caactcggcc agcttcctgg ccgctatctg ggcattggtg
     5101 ctgatcaaag ataccgaccg gccccggccg tcgatccgca ggccggtccg ggaactcttc
     5161 caggagggac tggagcatgt ctggcgggag cggccggtcc ggacgctgct cttccaggcc
     5221 gcgattctga gcgtcttcgg tttcatgggc cacacgctga tgccggtctt cgcccgcgac
     5281 gtgctcaacg ccggggtcaa gggcctggcg cacctgatgt cttcgctggg aatcggttct
     5341 ttgctgggtg cgttcagcgg catgctcttc agccaccacc cgaaccggga gcgccaggta
     5401 agcctgggcg gcctggtaat ggctgccggc ctgttcggac tggcctgctc acggtacctg
     5461 tggctttcga tgggcatgct gttgctggtc ggctggggag cggtggtcca ggcggtgacc
     5521 atcaactctc tccttcagca gatggcgccg gaacatctgc atgcccgggt ggttagtgtc
     5581 tacaccatgg tattttcggg cctgatgccc tttgggacct tcctggccgg tacgatcgcc
     5641 cagttctggg gcgccccggc ggcggtgatc ttcggcgcct ccgtctgcct ggtcggaagc
     5701 ttccattatc tgtcgctgac ttgcagaaag gggcgccggc ggccggcggt ttgattttta
     5761 gctgcgctga ggttaaaatt tatataataa ttaaggaggc gcgcccggga agcttttccc
     5821 gggccgcttt tgaaagcggg gaagggcgga gaagtctggt agccctttaa aatggttaga
     5881 aattctcagg atagcggtaa cgaatccgag atccggataa aatcagggcg tgaaagttac
     5941 ccggtagtga ttggcggccg cctggacgat ctggccggcc gcctctcaat tttcggggtc
     6001 ggacggctct ttttgatcac cgaccggcgc gtccacgaac tttacggcgc gaaacttctc
     6061 cgggatcttt ccggagccgg ctggcggacg gccgcggccg tcctgccgcc gggtgagcgg
     6121 accaagtcgc tttaccggct cgaacgtctt tatgcggccg gagtcgaggc cggcctggaa
     6181 cgcctttcgc tggtgctggc cctgggcggc ggggtcgtca ccgacctggc cggcttcttc
     6241 gcggccacct tcctgcgcgg tctgcccctg gtaaacgttc ccaccaccct gctgggccag
     6301 gttgacgcgg ccatcggcgg caagaacggg ttgaatctgc cggccggcaa gaacctggcc
     6361 ggaacctttt accagccgcg gctggtctac tgcgacctgg ataccctggc gaccctgccg
     6421 ttccgcgatt accggagcgg cctggccgag atcatcaagt gcggggtcat ccgcgacccg
     6481 gaccttttcg atctgatgga gcgcgggatc gatggtttta aaaggcggga tccgtacctg
     6541 cttcggcgcg cggttacggc cgcggttcgg gtcaagcgcg cactggtcga agccgatccg
     6601 ctcgaaagaa ccgggttgcg ccgggagttg aacttcggcc acactgtggg tcacggcctg
     6661 gagaaggccg gcggttaccg gcgtttccgc cacggcgagg cggtggccct gggcatgctg
     6721 gcggaggcgg aactggcccg gcgccgcgga ctgctggcgt cggccgcggc cgaccgcctg
     6781 ggacggctgg tggccgccta cggtttccgg acccgggccg ccgggtgtcc gctggaagga
     6841 gtcctgtccg ccctgcttca tgacaagaaa aagcaggatg gccgggttaa atttgtcctg
     6901 ccggcggcca tcggccggac ggttacctgc gatgacgtcg cccaggctga gctggcggct
     6961 gttctgaaga ttttcataaa ctgaaaaaag aaattactgt ttttcaccga ggcgcaagca
     7021 atgaagaaga gccgtttcaa gtggcaggaa gagaaggaga gcgacgagcc gccctggttg
     7081 tatgacggat cgagcctgat gcgcaacggc caggaggaag cggccgcccg gaaacgggcg
     7141 ggccgccgcc ggatgacgat cgggctggcc gtcctggccg cggttgtgct tttcctgctt
     7201 ctcttccgct ttgaggacgt ggtccgggcc ggcggcgagg tggaaccgct caacatcctt
     7261 tatatcaagg ctccgcttac cggcaccatc gatcagctgc ccttttccga ggggcaggag
     7321 atcaagcagg gacagctcct ggtaagcatc tggcccgagg aactggatat tttccgcgat
     7381 gttgagaata agcgctacga gttcgaaaac gccaaccgcg aattccagaa ggagaacgag
     7441 cggctggtaa tgctggcggt caagtacgaa cgcctgaagc gggagaaggc gatctttgag
     7501 ggcgacgact cggaagtccg ggttctccag gaacgcatcc aggccgccgg cgtcaccctg
     7561 gaccagcgca gcaaggacga gaagcgctcg aagaaccttt tccagcaggg tgtggtcagc
     7621 gccgccgagt acgaaaacgt tctggccaac ctggagttgt cccgctccgt ttacgccgaa
     7681 gctggagccc ggctgcacgc cctgaagaat gaacgggcca taaggataga gcagctcacc
     7741 cgcgacatcg agatgaccga aaaggagctt tcggtcagcc ggatctcctc cggccagtgg
     7801 gaggccgagc aggttcagaa atcctccatg ctggaggccg ccagcaagcg ggccgagctg
     7861 atgctgatca ccgccccctc cgacggccag atcgtccggc aggagaaaaa actgggcgac
     7921 cgggtcggca acggcgatct catctttgtc ctctccaccg gccgggaacg ccgtctctcg
     7981 gtgcggatcg acccggtcga ttccctgcgc gtggaggtcg gccaacccgt ccgggtttac
     8041 tcgagcctct tctcgcgacg ttacggctac gcgagcggcc gggtgattga actgggcacg
     8101 tatgcccgca gccaatccgg ccccgggctt cagacctata tcccggccaa ggtggcggtc
     8161 gaccagccgc cgtttccgct gctcctgggc acccgggtcg aggtgcagat actcaccggc
     8221 cgcaaacgga cgctcctctc gcgccgtccc tccggccgcg gtggcaagtg aacaacgccc
     8281 ccgagccggc cggcccggtt gagacgccga tcgtcctggg gtcggtggac cggatcccag
     8341 gcttgattct ccagcgtctc caggacaccg ccatccagga cgaagagatc ctgctggcgg
     8401 ccgaggccga ccttgacgaa cttggccata tcgagccggt ctggctgctg gccgggacga
     8461 agcacgtggt cgccgttccg gccgtcccgg accggcccct gcgcggtcct tacgcctacg
     8521 cggtcaccaa cgattttgac ctccacccga gcatcggctc cacctcggtc cgggtcttcg
     8581 tggagaagac tcccttcgaa ctggtccggt tcaccaacga gttccgcgaa cgcttccagc
     8641 gggtggtggt tcaacttaag cggattcgaa acaacgaacc gatccagccg gaggccctgc
     8701 tcaagctgaa tgaggacttc tgcccgaagt gcggcctgat cctgccccgt tccggcgccc
     8761 cctgccgccg ctgcgtcaag cacggggcgg tcttccagcg catgttcgcc atcctctggc
     8821 attatcggtg gttgatgctg gccctcttcc tctgcatgat cgccggcatc gtcctggaca
     8881 tgctgccgcc ctacctgacc cggatcctgg tcgacaacgt gctcggcagc cagtcccacc
     8941 cggaatggtt gttgtggctg gtggcctccc tggccggcgc ggccctgttg cgccgcggaa
     9001 tcgacgtgat catcagccag ctgaccccgc gggtcgggac ggcgatgacc aacgacatcc
     9061 gcaaacgcct cttccagaag ctccaggaga tgtcggtggc ctactacgac cgggtccagg
     9121 tcggcagttt gatgagccgg gtcatcaacg acgtggaagt gatgcacggc ttcgtcaccc
     9181 aggtcaccca gggcttcctg gtcaacctgc tgctgatcct cttcatcggc gtcatgctct
     9241 tctacctgaa cgcccggctc gccttcttcg tgctcatccc gatccccttc gtggtgatcg
     9301 ggaccctctt cttctggaac cgcatttacc cgaactacta caagtattgg gagagcaact
     9361 ccaagctggg cgccatgctc aacggcgtgc tgagcggcat ccggctggtc aaggttttcg
     9421 gccaggagca gcgggagacc agccgtttca accgttcggt cgactacctg cgcgaggccc
     9481 gcatccgggt ggacacccgg gtcggcgcct tttcgccggt gatggcctat ttcttcggac
     9541 tgggcggcct gatcatctgg ttcgtgggcg gccgggacgt gctctcgggc gtgatcaccc
     9601 tgggcaccct gatggccttc ctgggttaca tcggcatgtt ttacgcgccc ctgggcagcc
     9661 tggccatgtt ctccaactgg gtcacccagt tcgccaccgc cagccagcgc atcttcgaaa
     9721 tcctggacat gccctccgag accctggacg ctccggacgc gatcgactgc ccggatatcc
     9781 ggggcgacat cgagttcaag gacatctggt tcggctacga tccctacaac ccgattctga
     9841 agggcatttc gctcaagatc caggccggcg aaaagatcgg catcgtcggc cgcagcggtt
     9901 ctggcaaaac cacgctcatc aacctgctct gccgttttta tgacgtccag cggggccgaa
     9961 ttctcatcga cggcgaggat attcgccact tcaagcagac ccagctgctc cagcacctct
    10021 cgctggtcct gcaggagcct ttcctcttcc gaggttcggt ccgggagaat atcgcctacg
    10081 gacggcccga ggccactttc gaggagattt tgaacgcctc caaggcggcc aactgccacg
    10141 atttcataat gaagctctcc gaaggctacg acacaccgct cggggaacgg ggcgccggtc
    10201 tttccggcgg tgagaagcag cgcatctcga tcgcccgggc cttgctgtgc aacccgcgca
    10261 tcctgatgct ggacgaggcc acctccagcg tggacaccta aagcgagcag gagatccagc
    10321 gggcgctgga ggtcatcggc cgcggccgga cgctgatctc gatcgcccat cggctcagca
    10381 ccctgaagac cgccgaccgg atttacgtca ttgaccgggg gcagctggtc gagtccggca
    10441 accacgccga actgatggga aagcagggca cctattaccg gctggtccag atccagtcca
    10501 ccctggccag cctcgacctc aagtcctgag atcatggaaa agaaccggga atcggcagtg
    10561 gtcaagaagg tcatggatta cggcggcgtg gggatccgca tcctcaaggc cgaccgggtc
    10621 aagatctacc gggatatcga caaccttctc tacgccgagg tggccgaagc gggcctggag
    10681 acgccccggc tttaccgcgg cgttttcgcc gtcctctgct ttccggtctc cggaccggac
    10741 cgctacatat cgctgcgcta cgtgaatccc tccggccagg aggaggagat cggggtgatc
    10801 cgggagccga tcgagtttcc ccgcgaggac cggattcagc tggccaagag cctggcccag
    10861 tattacttcg agttcgagat cgaacgcatt gtgagcatcg aattcagata caacctgctg
    10921 atgttcgacg tgctgaccaa cttcgggccg cgccagttcg agatgcgctg gcgcggggac
    10981 cgggccctga gcctcggcga gaagggcaag gttctgctcg acgtttacga ttgccgctat
    11041 atcgtccgcg atgtttcggc cctgccccgc gaagaccagg ccctcttcaa ccgcttcatc
    11101 tattggtaaa tttgattcgg gcgcggaagt ttgacgcttc ttcagcttct gcattataat
    11161 tctaagttat ggacaaggaa atcgaaaagc ggcgtcaggc catcaacaag ctggacagcc
    11221 agctgctgaa gcttctctcc gagcgggcgg cccggattgt cgagatcggc cggctcaaga
    11281 aggagaaagg ccttaccttt tacgacccgg cccgcgaacg gcgcatcctg gaacggctga
    11341 agaaggagaa ccccggcccc ctgaattccg aggcggtggg cgccatcttc atggaggtca
    11401 tcagctcctg ccgtttcctg gagtacccga cccggatcgc ctacctgggg ccggaggcca
    11461 ccttcaccca ccaggcggcc ctgaagactt tcggcagcca ggccctcttc cttccccaga
    11521 agagcacctc ctcggtcttc agcgccgtgg agaaatccgg ggccgattac ggggtggtcc
    11581 cggtcgagaa ttccaccgag ggcgtggtca gccataccct ggacatgttc atggattccg
    11641 acctgaagat cgtcaacgag gtcctgctgc cggtccgcca ccacctgctg ggtttcggtt
    11701 ccctcaagga ggtccgggag gtttactccc atccgcaggc gctgggccag tgccagcgct
    11761 ggctggagga gaacctggtc aaggtgaccc tgcacgaaac cgagagcacc gccgaggcgg
    11821 cccgcctggt cagcaaccgc aagggggcgg ccgcgctggc ctcggagatc gccgccaccc
    11881 tttacggact gccgatcatg gtgcgcgacg tccaggacca cagccgcaat tacacccgct
    11941 tcctggtgat cggccgccac ttcagcggcc ggaccggccg ggaccgtacc agcattctct
    12001 tctccatcaa ggaccgggtc ggcgccctgc acgacatgct gctgcctttc caggggcaag
    12061 gcctgaacct taccaagatc gagtcgcgtc ccaccaagcg ccgcgcctgg gagtacgtct
    12121 tcttcgtcga ttttctcggt tacaaggacg acccggaggt tcgcgaggcc ctgtcggaac
    12181 tggaaaaact ctgccttttc ctgagagttc tcggttccta cccggtggcc gatgaaccct
    12241 gaggtgcggc tgccgctgct gctcctgctc ttcggacacg ttgtcgccga ctacctttgc
    12301 cagccccgct ccctgacctt tttgaaacgg cgccggccgg tcttcctgct gctgcacgga
    12361 ctggtggttt tggcctggct ctggcccctg gccattcttt acccgggacg ggcggttttg
    12421 cttctgctga cggcggtggc cgccagccac ctggccatcg acgcggttaa gatcggcctg
    12481 gagcgtcggt gctgtttcca gcggcgggag aagcggctgg ccaacgtcat cgaccaggga
    12541 ctccatttcc tggcgctggc ggccgcctgg tggctcggat tccggggccg gctctggccg
    12601 gcggcgcttc cccgaacgcc ggttttgttg aattcgctgc tggttttggt tataattcta
    12661 ataggcgtca aggccggttt cggttttctt gaaacaggaa gagaagatga tcataacctt
    12721 acgaccgggc acgactgaag aaggcgtacg cgaggttctt gagaagatca aggaccttgg
    12781 cttcaccccc catctctcct cgggcaagga gcgggtggtc atcggcgtga tcggcgaaaa
    12841 cgccattctt acccgcgagc agttcgaggc gatgttcatc gtcgaatcgg tgacccccat
    12901 ctcgcgcccc tacaagctgg tgagccggga gttcaagaag gagaacaccg tgatcaccgc
    12961 cggcggggtc gagttcggcg gttcggagtt cgtggtgacc gccggaccct gcgccatcga
    13021 gagtcgcgaa cagatcctgg agacggcccg ggcggtgcgg gcggccggcg cccgggtcct
    13081 gcggggcggc gccttcaaac cgcgcacctc tccctatgcc ttccagggcc tgggcgagga
    13141 ggggctcaag tacctggccg aggcggggaa ggaactgggg atgcccaccg tcaccgaggt
    13201 gatggatccg cgccaggtcg aactggtttg ccggtatgcc gacatcatcc agatcggcgc
    13261 ccgcaacatg cagaacttcg acctgctcaa ggaggtcggc cgggcgaagc ggccggtcct
    13321 gctcaagcgc ggcatgtcgg cccggatcga ggagttcctg atgtcggccg agtatattct
    13381 ctcccagggc aactaccagg tcattctttg cgagcggggc gtgcgcacct tcgaggacgc
    13441 cacccggttc actctcgacc tggcggcggt gcccctggtc aaggaactca gccacctgcc
    13501 gatcgtggtc gatcccagcc acggcaccgg ccggcgcaac ctgatcgccc cgatgtcccg
    13561 ggcggcgctg gccgccgggg ccgatggcct gctgatcgag gtgcatccca agccggaggc
    13621 cgcggtctcc gacggtttcc aatcgctgcg cttcgatgat ttcaagaagt tgatggcctc
    13681 gcttcaaacc ctggcgcccg tcctggagcg ccggatttga gggaaatccg ggaccgaaca
    13741 tgtcggaact gacggtccgg ccggttgacc gcctggccgg tgaaatccgg ctgcccggcg
    13801 acaagtccat ttcgcaccgc gccctcctta tctcggccct ggccgaggga aaatcaatca
    13861 tcaccggcct ccagtccggc gccgattgcc tggccacccg ccgcgtcctg gccggcctgg
    13921 gtgtccggat cgaaacgctt gccgatggct cggtcgccgt cttcggaagc ggcccggccg
    13981 gtttccgggc gtccccgtcg atcctcgact gcgccaattc cggcaccacc atgcgcctga
    14041 tggccggcat cgccgccggg ctgccctttg ccacttccct gaccggcgac gcctccctgc
    14101 gcaaaaggcc catggcccgg gtgaccgttc cgctgcggga gatgggcgcc cggatccagg
    14161 ccgagccggg cgagttgccg cccctaatca tccagggtgg ccgcctgacc ggccgggaac
    14221 accggctgac ggtcgccagc gcccaggtga agagctgcct gctgctggcc ggtttgctgg
    14281 ccgccggccg gaccacggtc atcgaaccgg ctgaaacccg ggaccacacc gagcgcatgc
    14341 tgggatactt tggcggccgg gtcgaacggg cgggccgcac cctctcgatc gagggcgggc
    14401 agcgcctggc cggccggccg gtccgggtgg tcggcgattt ttccgcggcc gcctttttcc
    14461 tggccgccgg cgccatcgtg ccggaggcgg aactggtgat tccggaggtg ggtttgaacc
    14521 cgagccggac cgccttcctg gagctgctcc ggaggatggg cgccgtgatc gaggcggagc
    14581 ccggcggcga gaccgccggc gaaccctccg gccgcctgg
//
LOCUS       DAOQYN010000017        14346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_4693_length_14346_cov_5.437548, whole genome shotgun sequence.
ACCESSION   DAOQYN010000017 DAOQYN010000000
VERSION     DAOQYN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14346
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4693_length_14346_cov_5.437548"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PKN80_01435"
     CDS             complement(<1..840)
                     /locus_tag="PKN80_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ34711.1"
                     /translation="MTETEVYRSYQRYIVPSYQRTPLCLVSGRGCWVTDLNGRRYLDF
                     FPGWAVSGLGHCHPRVVKAIREQAGRLLHVSNNYHHPLQAKLAAEIIKASFPGKCFFA
                     NSGAEANEGALKLARLHGRPEGRYQVISFRGSFHGRTLATLTLTGQAKVQKGFAPLPA
                     GFRNKAVFNDLASVEKLVNQRTAAVILEPIQGEGGVKPARPEFLAGLRRLCDREKLLL
                     IFDEVQSGLGRTGRLFAFQHYGIRPDAMTLAKTLGGGVPIGALVVAEKYADLLQPGTH
                     ASTF"
     gene            complement(859..1734)
                     /gene="argB"
                     /locus_tag="PKN80_01440"
     CDS             complement(859..1734)
                     /gene="argB"
                     /locus_tag="PKN80_01440"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNQ34712.1"
                     /translation="MEEATRKTQVLIEALPYILKFRDRYSVVKYGGSVLRENRFIRAV
                     LQDLVFMSTVGMRVILVHGGGSRISETLAARGIKSEFVNGLRYTDSSTIEIIREVLLE
                     VNGEIRRTIEELGGRTALLDPESRALKAVKLNRPGADLGLVGEISQVDKTFFEECGRN
                     GTIPVVPPVAFGADGFLYNINADTVASQIATALQAEKLVFLSDQPGVMRNRDNAESLI
                     SILRPSQARGLIEDGIITAGMIPKVQAALTAISQGVRKVHLVAGHIPHALLLEIFTTQ
                     GTGTEIVPEDYQNGI"
     gene            complement(1772..2551)
                     /gene="purQ"
                     /locus_tag="PKN80_01445"
     CDS             complement(1772..2551)
                     /gene="purQ"
                     /locus_tag="PKN80_01445"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HNQ34713.1"
                     /translation="MSAPRVLILRTAGTNCDLETEHGFRLAGAKPDRVHINRLLERSV
                     RLADYQALVIPGGFSYGDDLSAGKVLANELRLKLKDDLFAFAAAGRPVIGICNGFQVL
                     VKTGLLPGFNAGGGRLTLTFNDSGHFEDRWVHLRCAGASPLLEGLRNETISLPVAHGE
                     GKFLADGPETLERLKANGQVALQYATPEGEPTLEFPANPNGSEAAVAAVTNQAGNVLG
                     MMPHPERFLFYYSHPRWTRGEGKSQDGLRLIRSFVRYAAAG"
     gene            complement(2703..3521)
                     /locus_tag="PKN80_01450"
     CDS             complement(2703..3521)
                     /locus_tag="PKN80_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNQ34714.1"
                     /translation="MAETAKETGAYLEATRRRIDRYLKKSLPRAGAAPPRFLQAIDYA
                     VFSGGKRVRPILLLYTNRLCGGRTEKALPAAAAVELIHTFSLIQDDLPSMDNDDLRRG
                     KPTLHRAFEEYAALLASDYLLIRAFELLARHYPPEAALVTGRLIGLDGLVVGQYLDLA
                     GPGKPIDRRRLTEIRKKKTAALFELVFRLGALTAGRIRMADDWAEYGRDFGLAFQLWD
                     DLADEKKIEPAERMVRQLRRLNRELEKRQARLAPEAPVLDYFRNFLLQGAGPDR"
     gene            complement(3523..4065)
                     /locus_tag="PKN80_01455"
     CDS             complement(3523..4065)
                     /locus_tag="PKN80_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HNQ34715.1"
                     /translation="MKTVTLPMKKEDLAALEAGQEVLLSGPVYTARDRVHKIIFEEIT
                     RARRENRQPKLPFPLRGQTIYYCGPTPTPPGQIIGAAGPTTSSRMDPYTPILLEAGLQ
                     AVIGKGKRSPEVQAAFARFGAVYFITYGGCGALLASHVFRVEPIAFPELGPEAVWKLD
                     ILDFPARVGIDARGGKLRNL"
     gene            complement(4075..4947)
                     /locus_tag="PKN80_01460"
     CDS             complement(4075..4947)
                     /locus_tag="PKN80_01460"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNQ34716.1"
                     /translation="MKKTERIRLIKADRLIRLVTELARQANFEFPKPLVHLLDRAIVR
                     EESAEAKQALSAILTNIRLAQRLPLPLCQDTGLVEVFLEIGHRVRLDLGGGQDLETVI
                     NRGVSRAYREYYLRASAVDPLSRENTGDNTPAVVHTRIVAGRGLVIRMLIKGFGSENM
                     GRIQALSPGASPEEIVEKVVQAVAEAGANPCPPVIIGCGLGGTMEQAALAAREALLEP
                     EELLRPNPDRQLRAMEKEILRRVNRLGIGPAGMGGRTTALAARIEKRPTHIAGLPLAV
                     NISCWALRTARAEI"
     gene            complement(4910..5560)
                     /locus_tag="PKN80_01465"
     CDS             complement(4910..5560)
                     /locus_tag="PKN80_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNQ34717.1"
                     /translation="MSKKTSKPARVKPTLQNLQRIFLTGLFIIAPVAVSLWVLAILVR
                     FMENLLGPIFRNLLGETYIPGIGFVSLILLILLLGFVASNILGRRFLKNAQQFLEKVP
                     LFNKIYLTVRGISDEILGGEQQRAFKSVVLVPFPSPGIRTVGFITAAPTEAGPGVVGV
                     FVPTVPNITTGFYLIYPESEVQSTSLTIEEGIRLVISAGLSRNPGEKNGKNPPDQG"
     gene            complement(5579..6334)
                     /locus_tag="PKN80_01470"
     CDS             complement(5579..6334)
                     /locus_tag="PKN80_01470"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNQ34718.1"
                     /translation="MEKNPKAPPTAKRISRETILESFDHAFRGLLFCARRERNFQIHL
                     IIAGAVLVGSLFLNLSLLEFYVLILVITMVLVAEMVNTAFEKLADLVAPEQSRRVRDL
                     KNIGAACVLVVAAVSVLIGYLILGTHLAFYSPSALRAVQNSPWYLTVVALLATIVAVL
                     FLKRRLQSRSLFHGGMPSGHAAVSFALVTGIFFTSGGNLLVTALALPLAVLVAQSRVR
                     RDIHTWPEVIYGALLGAGMTTLIFQVLHLPTGK"
     gene            complement(6343..6777)
                     /gene="ybeY"
                     /locus_tag="PKN80_01475"
     CDS             complement(6343..6777)
                     /gene="ybeY"
                     /locus_tag="PKN80_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNQ34719.1"
                     /translation="MKSKKTAGELHWQNRQRKEKVNLKRLASRTLEVLAASGHPFRRV
                     GMVLLSDRAIAVYNSRFLGLNRPTDVLSFKLDPGEGEVLISAETARRQAEELGHSLEQ
                     ELMILAIHGFLHLQGFLDYKPADRRRMEAETRRLLRLAGRET"
     gene            complement(6683..7681)
                     /locus_tag="PKN80_01480"
     CDS             complement(6683..7681)
                     /locus_tag="PKN80_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNQ34720.1"
                     /translation="MKRITIIDQEIARQFFGQFDRNIRQLEKDFSVTIAGRGNELIIK
                     GARRKVLAAQQAVENRLASLEGRPAAAPVSPKPSQADRETEPAGETIQVGSKRTRIVP
                     RTEGQLRYVEAIRRSEIVVGIGPAGTGKTFLAVSLAIENLRENKVERIVITRPAIEAG
                     EKLGFLPGDLEEKIRPYLQPIYDALYDLLWPEEIRRLMEHRIIEILPLAYMRGRTLGD
                     AFILLDEAQNATPEQMKMFLTRIGVGSKAVVTGDITQSDLPMEQTSGLEDIEKFLKTI
                     PGIEFAYLDEKDVVRHPLVREIIRAYEIEKNRRRTSLAKPAAQRKSKPKTAGQQDA"
     gene            complement(7772..8542)
                     /locus_tag="PKN80_01485"
     CDS             complement(7772..8542)
                     /locus_tag="PKN80_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNQ34721.1"
                     /translation="MKIRLLIGVFVFLVSASWLAAQEGNQGYLLGGPSEALEPAARVT
                     ATPKTRLGQPRPAVTTEVKKAPAAAPAAAPVVTEVPTSAEIEAAKAKAIAETAMEISR
                     KAETTANEANKTSVESKSVSEQAISAANESIQKANESIDKVNKNIDRVNEVNKNLVTE
                     IARLKFDLEQKDKALEESITLQNEQYAALKTEIESMKIKTPETYRVKKGDFLIKIANK
                     DEIYGKGSEWKLIYRANREKIRNPNLIYPGQELVIPKK"
     gene            8755..8958
                     /locus_tag="PKN80_01490"
     CDS             8755..8958
                     /locus_tag="PKN80_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34722.1"
                     /translation="MLAVKHFVSGTTRQALVFLDNEVREWLQENKITEVKFIRETFGQ
                     APTGMSGTAENSLFVSIWYEKPE"
     gene            8988..9062
                     /locus_tag="PKN80_01495"
     tRNA            8988..9062
                     /locus_tag="PKN80_01495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9023..9025,aa:Arg,seq:cct)
     gene            complement(9415..9915)
                     /locus_tag="PKN80_01500"
     CDS             complement(9415..9915)
                     /locus_tag="PKN80_01500"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNQ34723.1"
                     /translation="MASQRIEIWYKPGVLDAGAAELGKDIQDLGIRNVDRLEVGVIYR
                     LDGQAKPTELDRIARELFADSITQVYCRGRRRRRPGIEEVEVFYKEGVTDTVGETALK
                     GIRDLGIKSVKKVATGRRYFLAGRGLDRAETERIARHLLANEVIQVCHFAGGRTKTKK
                     SAPERI"
     gene            complement(9896..10816)
                     /locus_tag="PKN80_01505"
     CDS             complement(9896..10816)
                     /locus_tag="PKN80_01505"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNQ34724.1"
                     /translation="MKNKLPIELLYTGKVRELYRLEPGKLLMVASDRISAFDFILPTP
                     IPEKGKILSQLSVFWFRRLESIIGNHLLIDNFRNFPECLRPFEKELAGRSLVVKEVRK
                     LRIEAVVRGYLAGSGWEEYRRSGTVCGHRLPAGLSLSNRLPEPIFTPATKEEVGEHDR
                     NISFEECAGLVGLEAARQLREKSLALYRTAAAYALERGVIIADTKFEFGYDPTGDLIL
                     IDEALTPDSSRFWESARYQPGREQESLDKQYVRNYLLEIKWPKEPPVPELPPEVVAQT
                     REKYLQLYRIITGRSLDDAEKGAEHGQPAH"
     gene            complement(10889..11713)
                     /gene="murI"
                     /locus_tag="PKN80_01510"
     CDS             complement(10889..11713)
                     /gene="murI"
                     /locus_tag="PKN80_01510"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNQ34725.1"
                     /translation="MNDLIGVFDSGLGGLTVVYNIQRALPKNRVVYLGDTARLPYGTK
                     SPETVNLFARQDTRFLLPFAPRLIVVACHTVSALALKNLKRDFLTSFVGMLEPGVRAA
                     LKETRHGRIGVIGTSATVKSGAYARELTRRNPRLKVFSQACPLFVPLVEEGFLSGPVT
                     EQVIAQYLAPLKRKKVDTVILGCTHYPMLKREIGRFLGDGVALIDPSEAVVEDIRLNL
                     AGQPGRPVRTTGAAGTRSGRKLQIYLTDLTPNFRRVAKAFLGKPILNLRKVGIDEN"
     gene            complement(11706..12941)
                     /locus_tag="PKN80_01515"
     CDS             complement(11706..12941)
                     /locus_tag="PKN80_01515"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNQ34726.1"
                     /translation="MKNFLRAGAASLLLISLFCLCPRPTRAADIPLEQQARSANVTLY
                     TFSGEKWITLPDLAFLLKGSVNHHAATGRTELKFSKHNLVLLPETNKVLYDNQVQEMK
                     QTPRLVAGVMAIPVNFATYLLNLAFIPADQRSETTFRPVPSGAGKTEGRESAAGDTGL
                     PENVTLQPGIAELISKTAAKSEIVLVLDPGHGGHDSGAIGSIRGLEEKLVNLDLALRL
                     ENYLKNRPKLKIFLTRKDDTFVSLPDRTKFANDNNADLLISIHTNSARYNRYTADGFE
                     TFYPRSKAAVLASNVSLETDEIAALLTPPSGDRVIQQSQFLAAHIQERLASWLITPDR
                     GIKKANFWMLKESGMTSVLVEVGFICNPNIEANLQQPEVRQAIARAIGEGLENYLDAK
                     PLKSPISVGGSVVEKPDNE"
     gene            13061..13132
                     /locus_tag="PKN80_01520"
     tRNA            13061..13132
                     /locus_tag="PKN80_01520"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13093..13095,aa:Cys,seq:gca)
     gene            13747..13818
                     /locus_tag="PKN80_01525"
     tRNA            13747..13818
                     /locus_tag="PKN80_01525"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13779..13781,aa:Gly,seq:ccc)
BASE COUNT         2469 a         4264 c         4628 g         2985 t
ORIGIN      
        1 gaaggtcgag gcgtgggtgc ccggctgcag caggtcggcg tacttttcgg ccactaccag
       61 cgcgccgatc ggaacgccgc cgcccagggt cttggccagc gtcatggcat cggggcggat
      121 cccgtagtgc tggaaggcga agaggcgccc ggtgcgtccc aggcccgact gcacctcgtc
      181 aaagatcagg agcagttttt cccggtcgca gaggcgccgg agtcccgcca ggaattccgg
      241 ccgggccggt ttgactccgc cttcgccctg gatcggctcc aggatgacgg cggcggtgcg
      301 ttggttgacc agtttttcga cactggccag gtcgttgaag accgccttgt tccggaaccc
      361 ggccggcagg ggggcgaagc ctttctggac cttggcctgg ccggtcaggg tcagcgtggc
      421 cagggtccgg ccgtgaaagg agccccggaa ggagatcacc tggtagcgcc cttccgggcg
      481 gccgtggagg cgggccagct tgagcgcccc ctcgttggcc tcggccccgc tgttggcaaa
      541 gaagcatttg ccgggaaaac tggccttgat tatctcggcg gccagtttcg cctggagggg
      601 gtgatggtaa ttgttggata cgtgaagcag ccggccggcc tgttcccgga tcgccttcac
      661 cacccgcgga tgacaatggc ccagtccgct gaccgcccag cccgggaaga agtccaggta
      721 acgccggccg ttcaggtcgg taacccagca gccgcggccg gaaaccaggc aaagcggtgt
      781 acgctggtat gatggcacaa tgtatcgctg gtaagaacgg tagacttcgg tttcggtcat
      841 ggccgttttc ctcgcggttc agatcccgtt ctggtaatcc tcgggtacga tctcggtgcc
      901 ggtcccctgg gtggtgaaaa tttccagcag cagggcgtgc gggatgtggc cggccaccag
      961 gtggaccttg cgcactccct gcgaaatggc cgtcagggcc gcctgcacct tcggtatcat
     1021 gccggcggtg atgatgccgt cctcgatcag tccccgggcc tgggatgggc gcaggattga
     1081 gatcaggctc tcggcgttgt cacggttgcg catcaccccg ggctggtcgc tgaggaagac
     1141 cagtttttcg gcctggaggg cggtggcgat ctggctggcc acggtgtcgg cgttaatgtt
     1201 atagaggaat ccgtcggcgc cgaaggcgac cggcgggacc accgggatcg tcccgttccg
     1261 tccgcactcc tcgaagaagg ttttgtcaac ctggctgatt tcgcccacca gtcccaggtc
     1321 ggcgcccggc cggttcagct tgaccgcctt cagcgccctc gattccgggt ccagcagggc
     1381 cgtccggccg cccagttcct cgatggtccg gcggatttcc ccgttcacct cgaggagaac
     1441 ctcccggatg atctcgatgg tactggagtc ggtgtaacgc aggccgttga cgaattcgct
     1501 cttgatgccc cgggcggcca gcgtctcgga gatccggctg ccgccgccgt ggaccaggat
     1561 gacccgcatg cccacggtgg acatgaagac caggtcctgg aggacggccc ggatgaaacg
     1621 gttctcgcgc agcaccgatc cgccgtactt gaccacgctg taccggtccc ggaacttaag
     1681 gatgtaaggc agggcttcga tcaaaacctg ggttttcctg gtagcttctt ccatgtctcc
     1741 ctttgccgga cggcaaggcc ccggcctttt ttcaaccggc ggccgcgtac ctgacgaagg
     1801 aacggatgag ccgcaatccg tcctgcgatt tcccctcgcc ccgggtccag cgtggatgag
     1861 agtaataaaa gagaaatcgt tccgggtggg gcatcattcc cagaacattg ccggcctggt
     1921 tggtcaccgc ggccaccgcc gcttccgaac cgttcgggtt ggccgggaat tccagggtcg
     1981 gttctccctc cggcgtggcg tattgaaggg ccacctgtcc gttggccttc aggcgttcca
     2041 gggtttccgg tccgtcggcc aggaattttc cctctccgtg ggccaccggc aggcttatgg
     2101 tttcgttccg caggccctcc aggagcggtg aggcacccgc gcagcgcagg tgcacccagc
     2161 gatcctcgaa atggccggaa tcgttgaagg tcagcgtcaa acggccgccg ccggcattga
     2221 atccgggcag caggccggtc ttcaccagca cctggaaacc gttgcagatg ccgatcaccg
     2281 ggcggccggc cgcggcaaag gcgaagagat cgtccttgag tttcagccgc agttcgttgg
     2341 ccaggacctt cccggccgag aggtcatccc cgtaagagaa gccgcccggt atcaccagcg
     2401 cctggtaatc ggccagccgg accgaccgtt ccagcagccg gttgatgtgg acccggtccg
     2461 gtttggcccc ggccagccgg aaaccgtgct cggtctccag gtcgcagttg gtgccggcgg
     2521 tcctgagaat caatacccgc ggtgcgctca tcgttttatg gctgcctttt gatccggaag
     2581 tctccgttat ccccgcgggt cataggcggg gatccgcaat acctgaaaaa ccatcaaaat
     2641 cgtaaaaata attattatac cattattggc aaaggcgata aaaaagacgc ggccgccggt
     2701 gttcaccggt ccggcccggc cccttgcagg agaaaattcc ggaagtaatc cagcaccggg
     2761 gcctccgggg ccaggcgggc ctgccgtttt tccagttccc ggttcaggcg gcgcagctgc
     2821 cggaccatcc gctccgccgg ctcgatcttc ttttcgtcgg ccaggtcgtc ccagagttga
     2881 aaggccaggc cgaagtcccg gccgtattcg gcccagtcgt cggccattcg gatccgtccg
     2941 gccgtgaggg cccccagccg gaagaccagc tcgaagaggg cggccgtctt cttttttcgt
     3001 atttcggtaa gccgccttcg gtcaatcggt tttcccggtc cggccaggtc aaggtattgg
     3061 ccgaccacca gcccgtccag gccgatcagc cggccggtga ccagcgcggc ctccggcggg
     3121 tagtggcggg ccaggagttc gaaggcgcgg atcagcaggt aatcggaggc cagcagggcg
     3181 gcgtattcct cgaaggcccg gtgcagggtt ggcttgcccc ggcgcaggtc gtcgttatcc
     3241 atggacggca ggtcatcctg gatgagcgag aaggtgtgga tgagttccac cgcggcggcc
     3301 gccggcaggg ccttttccgt ccgtccgccg cacaaccggt tggtgtagag aagcaggatg
     3361 gggcggactc gtttgccgcc ggagaagacc gcgtaatcaa tggcctggag gaagcgcggc
     3421 ggcgcggcgc cggcccgggg caggctcttc ttcaggtaac ggtcgatccg ccggcgggtc
     3481 gcttccaggt aggcgccggt ttcttttgcg gtttcggcca tgtcagaggt tccggagttt
     3541 gccgccccgg gcgtcgatgc cgacccgggc cgggaagtcg agaatgtcaa gtttccagac
     3601 cgcttccggt cccagttccg ggaaggcgat cggttccacc cggaacacgt ggctggccag
     3661 gagggctccg caaccgccat aggtgatgaa gtagaccgcc ccgaaacggg cgaaagccgc
     3721 ctggacctcg ggactgcgct tacccttgcc gatgaccgcc tgcagccccg cctcgagcag
     3781 gatcggtgtg taagggtcca tccggctgct ggtggtgggc ccggccgctc cgatgatttg
     3841 tccgggcggg gtgggagtgg ggccgcagta ataaatggtc tgtccccgga gtggaaacgg
     3901 cagcttgggt tgccggttct cccggcgggc ccgggtgatc tcttcgaaga taatcttgtg
     3961 cacccggtcg cgggcggtat agaccggacc ggagagcagt acctcctggc cggcctccag
     4021 ggccgcgagg tcctcttttt tcatgggcag cgttacggtt ttcatggaag tcgttcagat
     4081 ttccgcccgg gcggtgcgca gcgcccagca ggagatgttc accgccagcg gcagcccggc
     4141 gatatgggtg gggcgttttt caattcgcgc ggccagggcg gtggtccggc cgcccattcc
     4201 ggccgggccg atgccgagcc ggttcacccg ccgcaggatc tctttttcca tggctctcag
     4261 ttgccggtcg ggattgggac gcagcagctc ctccggctcc agcagggctt cccgggcggc
     4321 cagggccgcc tgttccatgg tcccgcccag cccgcagccg atgatgaccg gcggacaggg
     4381 gttagccccg gcctcggcca ccgcctggac caccttttca acgatctctt ccggactggc
     4441 gccgggggag agggcctgta tcctgcccat gttctccgaa ccgaaacctt tgatcagcat
     4501 ccggatgacc agcccgcggc cggccacgat cctggtatgc accacggccg gggtgttgtc
     4561 gccggtattt tcacggctca acggatcaac cgccgaggcg cgcaggtaat attcgcggta
     4621 ggcccgtgag acgccccggt tgattaccgt ctcgaggtct tggccgccgc ccaggtcgag
     4681 ccgcacccgg tggccgattt ccaggaagac ctcgaccagg ccggtatcct ggcagagcgg
     4741 caggggcagg cgctgggcca gccggatgtt ggtcaggatg gccgacaggg cctgcttggc
     4801 ctcggcgctt tcctcccgca cgatggcccg gtcgagcagg tggaccagcg gcttcgggaa
     4861 ctcgaagttg gcctggcggg ccagttccgt caccaaccgg atcagccggt cagccttgat
     4921 caggcggatt ctttccgttt ttttcacctg ggttccggga gagcccggcc gagatcacca
     4981 gccggattcc ttcctcgatg gtcaggctgg tggattggac ttccgattcc gggtagataa
     5041 ggtaaaaccc ggtggtgatg ttgggaacgg ttggcacgaa aaccccgacc actccgggcc
     5101 cggcctcggt cggcgcggcg gtgatgaagc ccaccgtccg gatgcccggg ctcggaaagg
     5161 gaaccagcac cacgcttttg aaggcccgct gctgttcgcc gcccaggatt tcgtcgctga
     5221 ttccgcgcac ggtcaggtaa atcttgttga agagcgggac cttttccaga aattgctggg
     5281 cattcttcag gaaacggcgg cccaggatgt tgctggccac gaaccccagc agcaggatga
     5341 gcaggatcag cgagacgaaa ccgatgcccg gaatgtaggt ttcgcccagc agattcctga
     5401 aaatcggtcc caggaggttc tccatgaagc ggaccagtat cgccagcacc cagagggaaa
     5461 cggccaccgg ggcgatgatg aaaagcccgg tcagaaagat gcgctgcagg ttctggagcg
     5521 tcggttttac ccgcgccggt ttcgatgttt tttttgacat agagaccttt tttaggggtt
     5581 acttgccggt tggcaggtgc agtacctgga aaatcagcgt ggtcattccg gcgcccagga
     5641 gcgccccgta gatgacctcc ggccaggtgt ggatatcacg tcgcacccgg ctctgggcga
     5701 ccagcaccgc cagggggagc gccagggcgg ttaccaggag attgccgccg ctggtgaaaa
     5761 aaatcccggt taccagggcg aaggatacgg cggcatggcc gctgggcatt ccgccgtgaa
     5821 acagggagcg ggactggagg cggcgcttga gaaagaggac cgcgacgatg gtggccagca
     5881 gggcgaccac cgtcaggtac cagggagagt tctgcaccgc gcgcagggcc gagggtgagt
     5941 agaaggccag gtgggttccc aggatgagat acccgatgag caccgagact gccgcgacga
     6001 ccagcacgca ggcggcgccg atgtttttga ggtcgcggac ccgccggctc tgttccgggg
     6061 cgaccaggtc ggccagtttt tcgaaggcgg tgttgaccat ttcggccacc agcaccatgg
     6121 tgatcaccag tatcagaacg tagaactcga gcaggctcag gttcaggaag agcgacccga
     6181 ccagcaccgc gccggcgatg atcaggtgga tctggaagtt cctttcccgc cgggcgcaga
     6241 agagcagtcc tcggaaggcg tggtcgaaac tctccaggat cgtttcccgg cttatcctct
     6301 tcgccgtcgg cggggccttc gggttctttt ccatgcccat tttcaagttt cgcggccggc
     6361 cagccgcagg agccgccggg tttcggcttc catccggcgc cggtcggccg gcttgtaatc
     6421 caggaatccc tgcaggtgca ggaaaccgtg gatggccagg atcatcagtt cctgctccag
     6481 cgaatggccc aattcctccg cctggcgccg ggccgtctcg gccgagatga ggacctctcc
     6541 ctcgcccggg tcaagcttga aactcaggac gtcggtcggg cggttcaacc ccaggaagcg
     6601 ggagttgtaa acggcgatcg cccggtccga aagcagcacc atcccgaccc gccggaaggg
     6661 gtggcccgag gccgccagaa cttcaagcgt cctgctggcc agccgtttta ggtttacttt
     6721 ttctttgcgc tgccggtttt gccagtgaag ttctccggcg gtttttttcg atttcataag
     6781 cgcgtatgat ttcccggacc agcgggtggc gtaccacgtc cttttcgtcc aggtaggcga
     6841 actcgatacc cggtatcgtc ttgaggaact tttcgatgtc ctccagcccg gaagtctgtt
     6901 ccatcggcag gtccgactgg gtaatgtcgc cggtaaccac cgccttcgat ccgacgccga
     6961 tccgggtcag gaacatcttc atctgctccg gggtggcgtt ctgggcctcg tccagcagga
     7021 taaaggcgtc gcccagggtc cgaccgcgca tataggccag cggcaggatc tcgatgatcc
     7081 ggtgttccat gagccgccgg atttcctcag gccagagcag gtcatagagg gcgtcgtaaa
     7141 tgggctggag atagggacgg atcttttctt ccaggtcgcc cggcaggaag ccgagtttct
     7201 cgccggcttc gatggccggg cgggtgatga cgattcgctc caccttgttt tcccggaggt
     7261 tttcgatggc cagggagacc gccaggaagg ttttcccggt gccggccggt ccgatgccca
     7321 ccacgatttc gctgcggcgg atcgcctcca cgtaacggag ttgcccttcg gtccggggta
     7381 cgatccgggt gcgtttcgaa ccgacctgga tggtttcgcc ggccggttcg gtttcccggt
     7441 cggcctggct tggcttgggc gagacaggtg cggccgccgg ccttccttcc aggctggcca
     7501 gccggttttc caccgcctgc tgggcggcca ggactttgcg ccgggcaccc ttgatgatca
     7561 actcgttgcc ccgtccggct atggtcacgc tgaaatcctt ttccagctgc cggatattgc
     7621 ggtcgaactg gccgaaaaac tgccgggcta tctcctggtc aatgatagtt atgcgtttca
     7681 tggagcggtc ttccgggtta tcctaacaag acagaaaccc cgggcggacg cccggggttc
     7741 tggggttgga gaaaaaaacc ggtggcggcg ttcatttctt gggaatgacc agctcctgtc
     7801 cgggatagat gaggttggga ttgcggatct tctcccggtt ggcccggtag atcagtttcc
     7861 attcggatcc cttcccgtat atctcgtcct tgttggcgat cttgatcagg aaatcgccct
     7921 tcttgacccg ataggtttca ggggtcttga ttttcatcga ctcgatttcg gtcttgagag
     7981 cggcatactg ttcattctgc agcgtaatcg attcttccag cgccttgtcc ttctgttcca
     8041 ggtcaaactt caggcgggcg atttcggtca ccaggttctt gttgacctcg ttgacccggt
     8101 cgatgttttt gttgaccttg tcgatcgatt cattggcctt ctggatcgac tcgttggccg
     8161 cgctgatggc ctgttcactc accgatttcg actcgacgct ggtcttgttg gcctcgttgg
     8221 cggttgtctc ggccttccgg ctgatctcca tggccgtctc ggctatggcc ttggccttgg
     8281 cggcctcgat ttcggccgag gtcggcactt cggtgactac cggggccgcc gccggggccg
     8341 ccgccggcgc ttttttcact tcggtcgtaa ccgcgggccg gggttgaccc aggcgcgtct
     8401 ttggagtggc ggtcacccgg gcggccggtt ccagcgcttc cgacgggcca cccagcagat
     8461 atccctggtt gccttcctgg gcggccagcc atgatgcgga aacaagaaag acgaagactc
     8521 ctatgagtag cctgattttc attctgcctc cgtagagacc aggacagaaa ggttgccaac
     8581 gttattattc tacttctgaa gccggttgac tgtcaatccg gccggtccgt tttccatctc
     8641 gacggtgatt ctggtataat cttacttaaa gccaggaccc ggattaccgg aaggcgatcg
     8701 gccgggaggg gcaagggcag gatttcctgt tctctggaaa gggagggggt ggcaatgctg
     8761 gcagtgaaac attttgtctc cggcactact cgacaggccc tggtttttct tgataacgaa
     8821 gtaagggaat ggctccagga gaacaagatc accgaggtga aatttatcag ggagaccttc
     8881 ggacaggcgc cgaccggcat gagtggaacc gcggaaaact cgctttttgt atccatctgg
     8941 tatgagaagc cggagtaacg gcctctattc ggttcccgcc tgaccctgcg cctgtagctc
     9001 agcccggata gagcaaggga ttcctaatcc ctgtgccgga ggttcaaatc ctctcaggcg
     9061 cgcttttttt taggagatgt ccggtcggcg gtggtggatt tgaaccgacg gcacggaagg
     9121 ggtgccgggg aagagggctt ccccggcgtt tttcgtattg gggatggggc ccaggaaggg
     9181 aagccccctt cctggaacgg agccggcccg gcggagtgtg gcgggaggtt caaatcctct
     9241 caggcgcgct tttttatttt tgttgagtcg gcgtggtttt ggatttgaac cgacggcacg
     9301 gaaggggtgc cggggaagag ggcttccccg gcgtttttcg tattggggat ggggtccagg
     9361 aagggaagcc cccttcctgg aacggagcgg atccggcgga gtgtggcggc aggttcaaat
     9421 cctctcaggc gcgctttttt tggtttttgt tcttccgccg gcgaagtggc agacctggat
     9481 tacttcattg gccagcaggt ggcgggcgat ccgttcggtt tccgcccggt ccaggccgcg
     9541 gccggccaga aagtaacggc ggccggtggc cacctttttt acgctcttga tacccaggtc
     9601 ccggatgccc ttcagggcgg tttcgcccac cgtgtcggtc actccttcct tgtagaagac
     9661 ctcgacctct tcaatgccgg gccgccgccg gcgtcttccc cggcagtaaa cctgggttat
     9721 ggaatccgcg aagagttccc gggcgatccg atccagttcg gtcggtttgg cctggccgtc
     9781 gagccggtag attactccca cctccagccg gtccacgttg cggatgccca ggtcctggat
     9841 atccttgccc agttccgcgg ccccggcgtc aaggacgccc ggtttgtacc agatttcaat
     9901 gcgctggctg gccatgctcg gctccttttt ccgcatcgtc gagactgcgg ccggttataa
     9961 tccggtaaag ctggagatac ttctcccggg tttgggccac cacttccggt ggcagttcgg
    10021 gcaccggcgg ctctttgggc cacttgattt ccagcaggta attgcgcacg tactgcttgt
    10081 ccaggctctc ctgttcccgg cccggctggt agcgggcgct ctcccagaag cgggaggagt
    10141 ccggggtcag ggcctcgtca atcaggatca ggtcgccggt cggatcgtat ccgaattcaa
    10201 acttggtgtc ggcgatgatc accccgcgtt ccaacgcgta agcggccgcc gtccggtaga
    10261 gggccaggga tttttcccgg agttgccggg ccgcttccaa tcccaccagc ccggcgcatt
    10321 cctcgaagga gatgtttcgg tcgtgttcgc ccacctcctc cttggtggcc ggggtgaaga
    10381 tcggttcagg cagccggttg gagagactca gaccggccgg cagccgatgg ccgcagacgg
    10441 tgcccgaccg gcggtactcc tcccagccgc ttccggccag gtagccccgg accaccgcct
    10501 cgatgcgcag cttccgcact tccttgacca ccagggaacg gccggccagc tccttttcaa
    10561 aagggcgcag gcactccggg aaattccgga agttgtcaat cagcaggtgg ttgccgatga
    10621 ttgattccag gcgccggaac cagaaaacgg agagctggct gaggatcttc cccttctccg
    10681 ggatgggcgt gggcagtatg aaatcgaagg ccgaaatccg gtcgctagcg accatcagaa
    10741 gtttgcccgg ttccagccgg tagagctcgc gcaccttgcc ggtgtaaaga agttcgattg
    10801 gcagcttgtt tttcattttt acctccgggg aagcggaggg ccggatgatt tttatggatt
    10861 gtggttcgcc gctccggcgg cccggccttc aattttcgtc gatgccgacc ttccgcaggt
    10921 ttagaatcgg cttgccgaga aaagctttgg cgacgcgccg gaagttcggg gtcaggtcgg
    10981 tcaggtagat ctgcaatttc cggccggatc gggttccggc cgctccggtg gtccgaactg
    11041 gccggcccgg ctggccggcc aggttcaggc ggatatcctc gaccacggct tccgacgggt
    11101 ctatcagggc cacgccgtcc ccgaggaagc ggccgatctc ccgcttgagc atcgggtaat
    11161 gggtgcagcc caggatgacc gtatccacct tctttcgttt caacggggcc aggtactggg
    11221 cgatcacctg ctcggtgacc ggtccggaga gaaatccctc ttccaccagg ggaacaaaga
    11281 gcggacaggc ctggctgaaa accttcagcc ggggattgcg gcgggtcaat tcccgcgcgt
    11341 aggcgccgct ctttacggtg gcgctggtgc caattactcc aatccggccg tggcgggtct
    11401 ctttcagggc ggcccggacg cccggttcca gcatcccgac aaaggaggtc aggaagtccc
    11461 gcttgaggtt cttcagcgcc agcgcgctaa cggtatggca ggccaccacg atcagccggg
    11521 gtgcgaaggg aagcaggaaa cgggtgtctt gccgggcgaa gaggttgacc gtttcggggg
    11581 acttggttcc gtaaggcagc cgggcggtgt cgccaaggta aacaacccgg ttcttcggca
    11641 gggcgcgctg aatgttgtaa acaaccgtga gtccgcccaa cccggagtcg aaaacgccga
    11701 tcaggtcatt cattatcggg cttctcaacc acgctgccgc ccaccgagat tgggctcttc
    11761 aacggcttgg cgtccaggta gttttccagg ccttcgccaa tggcccgggc gatggcttgg
    11821 cggacctccg gctgctgaag gttggcctcg atattcggat tacagataaa gccgacctcc
    11881 accaggaccg aggtcatgcc gctttcctta agcatccaga agttggcctt cttgatgccc
    11941 cggtccgggg tgatcagcca gctggccagc ctttcctgga tgtgcgcggc caggaactgg
    12001 ctttgctgga tgacccggtc gccggaaggc ggggtcagca gggcggcgat ttcatcggtc
    12061 tccagcgaaa cgttgctggc cagcacggcc gccttgctcc gcgggtaaaa cgtctcgaaa
    12121 ccgtcggcgg tataacggtt gtaacgggcg gaattggtat ggatggagat gagaaggtcg
    12181 gcgttgttat cattggcgaa cttggtgcgg tcgggcaggg agacgaaggt gtcatccttg
    12241 cgggtcagga atattttcaa cttcgggcgg ttcttcaggt aattctccag gcggagggcc
    12301 aggtcaaggt tgaccagttt ttcctcaagg ccccggatgg aaccgatggc tcccgaatcg
    12361 tggccgccgt gtcccgggtc caggaccaat acgatttcgc tcttggcggc cgtcttggag
    12421 atgagttcgg caatgccggg ctggagggtt acattttccg gcagtcccgt gtcgccggcg
    12481 gccgattctc tgccttccgt ttttcccgcg ccgctgggga ccgggcgaaa agtcgtctcc
    12541 gagcgttgat cggccgggat gaaggccagg ttgagcaggt aggtggcaaa atttaccggt
    12601 atggccatga ctcccgcgac caggcgcggt gtctgtttca tttcctggac ctggttgtca
    12661 tagagaacct tgttggtctc cggcagcagg accaggttgt gtttggaaaa cttcagttcg
    12721 gttcggccgg tggcggcatg atggttgacc gagcccttga gcaggaaggc caggtcaggc
    12781 agggtgatcc acttttctcc cgagaaggta tagagagtta cgttggccga acgcgcctgc
    12841 tgttccagcg ggatgtcggc ggcccgggtc ggccgagggc acaagcaaaa caggctgata
    12901 agcagcagac tggcggcccc ggcccggagg aaatttttca taattatata tttaaatcac
    12961 atttgcggct tgaaagtcaa caaagacgcc cgtcgccgcc gttttaaccg cgggttttct
    13021 ttgacaccat tccgtttgaa ccgatataat tataggtcac ggcggcatag ccaagtggta
    13081 aggcagcggt ctgcaaaacc gcaattcccc ggttcgaatc cgggtgccgc ctcagggcgc
    13141 cgagggtccg gactcggctc gggcgctttg ccgcggtgga aaggtacgct taaatccggc
    13201 gcgcggctaa ctcgccgtcc gcttggggcc ggaaggcggc cgtcgcggcc tggctcagac
    13261 ataagccgcg agaggatatc aatgccggac cgctgtcgct tttaagcttc tttcccgagg
    13321 ccgcggctcc gcgactcgtc tcgttccggg ccctcggctt caagggaaaa gacgaaacgc
    13381 aacaacaaga aggcttgcgt ctcggatact cattccctcg tttcgcttgt tttgcacccg
    13441 gactccgcga cgccgctcac cgaagaccgc cgcctacggg gaacagacga aaggcaaaaa
    13501 caaggaaaag gcaaaagctc cgcgactcgc ctcgttccgg gccctggata ctcattccct
    13561 cgtttctttt gcctcccgcg actcgccttc ttccgttgat gcctcccgtg aattcttagt
    13621 ggtctggaga tatttcggcg tttatcttgc cggcggccgc cgcctccttc atttgataaa
    13681 actccgcctt tgattgtaga ataaaacatt gcaaggcggc tcccgggttg tccggaaccg
    13741 ccgaacgcgg gaataactca gcggtagagt acgagcttcc caagctcgga gtcgcgggtt
    13801 cgaatcccgt ttcccgctct taagcttctt ggtagggatt cgatgggagc gaagtcaggc
    13861 gacgcgactg attaaggagc gttgccagcg ggtggccgac ccgaggcgta gtgaaagccg
    13921 agcggcgccg aatcccgttt cccgctctta agctgtctgg tagggattcg aagagagccg
    13981 tgaagtagag cgcttcgacg aactcagcgc acacttcacg gccatgaagc gtgcttcgct
    14041 ctgcttcatg gcgattattg tttctgaatc ttattcagac aaaccagcga atggccgacc
    14101 cgagccgcag tgggaggcga gggcgccgaa aattgagtat taagctggag atttttgttt
    14161 ttctcttggg ccgagggttt cgggtgaggc gagtcgcgga gccgcggcct cgggaaagaa
    14221 gcttaaaagc gtaagcggtc cggcataatc tccgcttgcg gcttctgttt gagccaggcc
    14281 gcgacggccg acttccggcc ccaagcggac ggcgagttag ccgcgcgccg gatttaagcg
    14341 tacctt
//
LOCUS       DAOQYN010000018        14323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_4699_length_14323_cov_4.640594, whole genome shotgun sequence.
ACCESSION   DAOQYN010000018 DAOQYN010000000
VERSION     DAOQYN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4699_length_14323_cov_4.640594"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PKN80_01530"
     CDS             complement(<1..631)
                     /locus_tag="PKN80_01530"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNQ34727.1"
                     /translation="MAQTKKLGVGIIGFGTVGSGVARILLERGDRLAEKTGLRVELRA
                     VCDSDLRRKRPVSLPPGLLSSEIETVLENPAVDVVAELVGGLEPAGSLIVRAFRNGKS
                     VVTANKALLAERGDRLFAAAREHGVRFGFEAAVGGAIPIIRALRESFVSNRVTSLYGI
                     LNGTTNFILTRMEEECLSLEGALKIAQANGFAERDPSLDLSGQDASHKLS"
     gene            complement(761..1873)
                     /gene="dnaJ"
                     /locus_tag="PKN80_01535"
     CDS             complement(761..1873)
                     /gene="dnaJ"
                     /locus_tag="PKN80_01535"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNQ34728.1"
                     /translation="MAEKRDYYEVLGLDRQATPESIKEAYRKQALQYHPDRNPGNKAA
                     EEKFKEATEAYEVLSDANKRATYDQFGFRGLEGAGFNPSDDLGRVQHDFSDLFANSDL
                     LEGIFNSFGIGGGRRGRRVMQGEDLRASVSISLPEAAEGVRKEIEVGRLVACPECRGS
                     GAAAGTKAAPCKQCGGHGQVTYNQGFFTMSRTCPICGGTGQMIEKPCRGCSGQGRVRR
                     REKLEVTVPPGVDHGSRLRVRGKGNVGPNGGPAGNLYLDIYLEPDPRFKREGSHLIYE
                     LPVTFTEAALGAEKSVPTLKGSVRMKVPAGTQSGTILRLRGRGFPDPSGYNRGDQLIR
                     VELETPVNLGREERRLLEDLENRTGRRAYPRSKSDR"
     gene            complement(1931..3916)
                     /locus_tag="PKN80_01540"
     CDS             complement(1931..3916)
                     /locus_tag="PKN80_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34729.1"
                     /translation="MAIARMERVHLFLPEARKEELIREMESFGLVEVGRLEPEEADLG
                     PAEADPGREADETLNRLNYLAAALGLESGAEPVRLRESDLPELLRTFPIEATVQAVRR
                     REKRLTALAGRDERLERIGRELEPFAWLDLPLKSLRGGGRVALQLVRVDRNRTAELVA
                     GLDKIGGHAQPLAEEKNRSLLFVAADRRQERELSETLNRLRAETVALPAYRTSPAATL
                     ARLERLRERNRNRRRAAEAAAAEFRSRRSEILALLDHYRQRQLELEAKRKLAATGRTL
                     LLTGWIRTQDRPRLEKFLAGTFPEAGLLARPARPEEAVPVALENGPVGQPFEAVVDLF
                     GRPVYSGYDPSAFVAFFFILSFAFCLGDVGYGLLLALGSFLLMKKFPAPAARRLLGVF
                     LYSGLATMLVGLLTNSWFGNLTSLFPPLAPLARFQSRLALFSPVDNPADTMKLFYFSL
                     VVGFVQITTGMLLKAKEAIRRFGALGALEGIPPLVIQLGLPFLLFTLVMRGRLPWAEP
                     AIPVTGLVLGVAALLIIYYQWRTQSDLVLKIFFAPYTLYGLVVGNLLGDTLSYARIFA
                     LGLTGGLLAVTVNQLAGMTGAIPVIGTLFFLLLLLGGHAFNLFISTLGAYVHTSRLQY
                     LEFFTKFYESGGRPFDPLRKQYRYLQPDGNPAAGPGT"
     gene            4101..5168
                     /gene="carA"
                     /locus_tag="PKN80_01545"
     CDS             4101..5168
                     /gene="carA"
                     /locus_tag="PKN80_01545"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNQ34730.1"
                     /translation="MEKACLALEDGLVVEGGSFGAPGEVTGELVFNTAMTGYQEVLTD
                     PSYRGQLVMMTYPLIGNYGVNDEDVESAGVQVEGFIVRENARHYSNWRARQSLSDYLK
                     AAGVPGIESIDTRMLTVHIREQGSLKAVLSTRDFDHQRLVARAKAAPGVVGQNLVIRV
                     SAEKPYYWNREGAGKAVVLDCGVKYSILRCVAAAGYRVLVLPCGAPAEAILSERPDLV
                     VLSNGPGDPAALGSIVAEVRKLLGRVPIYGVCLGHQILGQAVGGRTYKLKFGHHGGNH
                     PVRDLTGGRVMITVQNHGFCVDAETLPAGIRITHLNLNDESLEGMESREERFASVQFH
                     PEAGPGPHDARVFFERLRQGF"
     gene            complement(5212..6093)
                     /locus_tag="PKN80_01550"
     CDS             complement(5212..6093)
                     /locus_tag="PKN80_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ34731.1"
                     /translation="MFFSGFADEAGKDIETQIRATLELGWRHIETRAIGDQNITLIGQ
                     AEFEAVAEKLAAAGITVSCLGSGIANWAKKITEPPDSSYAEMKQAIPRLKKLGTRFIR
                     IMSFAVPPEVLDRDWSAEAIKRIREIVRMAEDGGVVCLHENCSGWGALSYEHTLRLLE
                     AIPSPSLKLVYDTGNPPVHDDVRGRPPYRKQDAWEFYRNVRPYIEYVHVKDDYLKDGK
                     EVYTFPGEGKGYVRETLTDLLKTGYDGGFSIEPHMAVVFHDAAVTSSDEIRYRNYIEY
                     GRRLEKMLAEIKAELGR"
     gene            6314..8773
                     /locus_tag="PKN80_01555"
     CDS             6314..8773
                     /locus_tag="PKN80_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNQ34732.1"
                     /translation="MSGFDRFTNQVRRMLDMARDEAIRFGQIEIGTEHLVLAMLRDGQ
                     GVAAAALTSLGYDLDFLRLEIEKVIAVGPALRIDGPLPLSRRAKRSLELGVEEARQYG
                     FNYIGTEHIILGILREGENQASAVLGRLNLTLERSRKAIYELVKPPLQGGETAARSKT
                     PALDSFGRDLTRMARDGRLDPVIGRAAEIERLVHVFCRRRKNNPVLVGEAGVGKTAIV
                     EGFVQAMVEGRLPEAVESRRVVMLDLAALVAGTKYRGEFEQRLKTVLQEVVENQVVIF
                     VDELHTLIGVGGAEGAMDASHILKPALASGEIQCIGATTMNEYRKHIEKDAALERRFQ
                     AIFVSPPDTGQTMEILKGLRPRYAEHHRVEISDTALWAAVQLSDRYIAGRFQPDKAID
                     LLDEACASVRLRAVRLPDNLRRMEAQLKALTEKREKATRDLDYETAISLREQEKTERV
                     GFDLLKKRWRLSISPTEGVVTEQDISEVVSRWTGVPVRQISEDEGARLLHMEEELQRQ
                     VIGQSEAVSAVCRAIRRSRTGIKDRRRPVGSFLFLGPTGVGKTLLARVLAGFLFDDQE
                     ALIQIDMSEYMERFSVSRLIGAPPGYVGYEEGGQLTERVRRRPYSVILFDEFEKAHPD
                     VYNLLLQILEDGHLTDSFGRRVDFRNTILILTSNLGTGSLQETASVGFVSDGGQPADY
                     EQTRQLLKNEAERVFRPEFLNRLDEMVVFRSLTDRDAAAIVRLEVDRVLVRLREQGLG
                     ISLSESAFRFLEREGFDPKLGARPLRRAITRHLEDRLSEEMLSGRLRTGGRVRVGMER
                     SGRRLSFDIEHPAAETIGTAH"
     gene            8844..9818
                     /locus_tag="PKN80_01560"
     CDS             8844..9818
                     /locus_tag="PKN80_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34733.1"
                     /translation="MIKVAVVGAGHMGSGHIRAYRACAGARLRYVCEPEEARGRSAAE
                     AAGAGWAADYRQVIDQVDAVSVAVPTNLHFRVASDFLAAGKAVLLEKPIADRLEDAEA
                     LVRMSRERGAVLQVGHIERFNPAVRELRRRLRDPRYLEIHRLGTFKGRGVEVSVVSDL
                     MIHDIDLALDLVDRPLLEVSALGMAAFTATPDIATGRLVFEGGRVANLTASRISDKSL
                     RKVRVFEMDCYWSLDCEHRELVCYRKRPESDWRGKARPTLADLISREVLAVEAADPLQ
                     RELESFLTAVREGGQPEVTGEDGRRALEVALQIEKSIANNQPVSSGFS"
     gene            9864..10448
                     /locus_tag="PKN80_01565"
     CDS             9864..10448
                     /locus_tag="PKN80_01565"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNQ34734.1"
                     /translation="MIEHASREARFPHDFTAKHIFLTKGVGRDKEKLTSFEQALRNAG
                     IAPFNLVHISSIFPPGARLITRERGLKMLKHGQILFCVMSRNETNEPHRLITASLGLA
                     IPKNTEKYGYLSEFSGFGMTDERSGDYAEDLAATMLATILGVKFDPDSSYDEKKQVWR
                     LSREIVRTTNVTQSAVGDKNGRWTTVVTAAVFVL"
     gene            10477..11358
                     /gene="speB"
                     /locus_tag="PKN80_01570"
     CDS             10477..11358
                     /gene="speB"
                     /locus_tag="PKN80_01570"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNQ34735.1"
                     /translation="MEIPYTFAGWPPRRLEEARAAILPVAYDATTSYRSGTRFGPQEI
                     ILASRFLEMYDEALGLEPGGPGVVTLPELWPDLASPEAALREVQAYVDGLLEKNLFPV
                     VLGGEHSITYGAVQAVRKFHPGLSVLVLDAHADLRQDYQGTRYNHACVSRRILDLGVP
                     VTVLGVRSLPSEDLPLLESGKEPGLNLAWASQLKAEPDWLGRFGDRLTETVYLSIDLD
                     FFDPAYVPAVGTPEPGGLGWDETAAFLAGLAGRRRLVGFDVVELDGSVHHTPSAYLAA
                     RLVHRLLGLCLPAADRY"
     gene            11454..12089
                     /locus_tag="PKN80_01575"
     CDS             11454..12089
                     /locus_tag="PKN80_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNQ34736.1"
                     /translation="MADLQAIANGVISGKAADVKNLVEAAIKEGVGAGDILKKGLIAG
                     MDVVGVKFRANEFYVPEVLIAARAMNAGMEVLQPELVKSGVKAIGKVGVGTVKGDLHD
                     IGKNLVVMMLKGAGFEVVDLGIDVPEDKFVQAVRDGVQVLGLSALLTTTMPAMKSVIE
                     AIKTAGLRDQVKIMIGGAPVTQAYASEVGADAYGEDAATAAIKAKELLGLN"
     gene            12100..12696
                     /gene="plsY"
                     /locus_tag="PKN80_01580"
     CDS             12100..12696
                     /gene="plsY"
                     /locus_tag="PKN80_01580"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017297485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNQ34737.1"
                     /translation="MDLSNIGLIAGAYLLGAIPFGYLAGRLAGVDVRKAGSGNIGATN
                     VLRTVGRGPAAATLLLDILKGFLPVWLARRPWALPPAWVAAIALAVVAGHVFSPFLKF
                     RGGKGVATAVGVLLGISPPAALAAVAVFGLVFGLGRIVSLASLAAAVLLPFLLGLLAP
                     EPAYAIFGGLVGALVVLRHRANLDRLLSRSEPRTQWKK"
     gene            12684..13667
                     /locus_tag="PKN80_01585"
     CDS             12684..13667
                     /locus_tag="PKN80_01585"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNQ34738.1"
                     /translation="MEKIAVVGAGAWGTTLALLLAGPDRSVLLWEKFPDYARQLAGER
                     ENVKFLPGFELPENLDVTDDLALAASAPAVVLVTPSFACRETAGRLAAAGCRGPVLVA
                     TKGLEAGSGLRMSQVVAEAMPGVAWSVLSGPTIAREVAAGRPTAAVCAGPDEKLNFFF
                     QDLFSTGHFRLYTSADAAGVEFGGALKNPLAIAAGIVEGLGLGDNAKATLLCRGLAEM
                     ARLGTALGGQERTFFGLSGLGDLITTACSPQSRNHRFGVAIGGGQPIEAYLKGQESVV
                     EGYHSARPALKLAAGAGVEMPIVSQVAAVLFEGKPPAAAVRELMGRPLKAE"
     gene            13676..14044
                     /locus_tag="PKN80_01590"
     CDS             13676..14044
                     /locus_tag="PKN80_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNQ34739.1"
                     /translation="MAEQAERGGEKVYYRIGEVSRRIGVAAHTIRYWEKEFRLGRPRR
                     NSRGHRVYTGAEVAKLLRLKELLYGRGLRISAAKRVLRESGSKSRQAAADQRPLRRLA
                     QELRRLEKNLEQLARLEAED"
     gene            complement(14058..>14323)
                     /locus_tag="PKN80_01595"
     CDS             complement(14058..>14323)
                     /locus_tag="PKN80_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ34740.1"
                     /translation="AGSPVLILDEATSNLDSESERLVQEALEKVMADRTCLIIAHRLV
                     TLQKSDWIIVMEKGRIAEEGTHAQLMERRGRYYYLYTLQQQKN"
BASE COUNT         2248 a         4626 c         5045 g         2404 t
ORIGIN      
        1 ccgagagctt gtgcgaggca tcctggccgg agaggtccag actggggtcg cgttcggcga
       61 agccgttggc ctgggcgatt ttcagcgccc cttccaggga gaggcactcc tcttccatcc
      121 gggtcaggat gaagttggtg gtcccgttca ggatgccgta aagggaggtg acgcggttgg
      181 agacgaaact ttcgcgcagg gcccggatga tggggatggc gccgccgacc gcggcctcga
      241 agccgaagcg gacgccgtgc tcgcgggcgg cggcgaagag acggtcgccc cgctcggcca
      301 gcagagcctt gttggcggtg accaccgact tgccgttccg gaaggcgcgg acgatcaggg
      361 agccggccgg ttcgaggccg ccgaccagtt cggccaccac gtccaccgcc gggttctcca
      421 gcaccgtttc gatttcggag ctgagcaggc caggcggcag gctgaccggc cgtttccggc
      481 gcaggtcgct gtcgcagacg gcccgcagtt ccacccgcag gccggtcttc tccgccagcc
      541 ggtcgccgcg ttccaggagg atcctggcca cgccgctgcc gacggtgccg aagccgatga
      601 tgcctacccc gagtttcttg gtctgggcca tgccattctt cgtgtgaacc ggccggaagc
      661 gatggagagg cagctgcggg agagtccggc cgccggtaac cacgggacgg gaacgggccg
      721 aaaaaagctt accttaacat tataacacgg cgccgccggt tcagcggtcg gatttgctcc
      781 ggggataggc gcggcggccg gtccggttct ccaggtcctc gagcaggcgc cgttcctcgc
      841 ggccgaggtt gaccggcgtt tcgagctcga cccggatcag ctggtcgccc cggttgtagc
      901 cggacgggtc ggggaagccg cggccgcgca ggcgcaggat ggtgcccgac tgggtgccgg
      961 ccgggacctt catccggacg ctgcccttga gggtcggcac gctcttctca gcgcccaggg
     1021 ccgcctcggt gaaggtgacc ggcagttcgt agatgaggtg gctgccctcc cgcttgaagc
     1081 gcggatcggg ttccaggtag atgtcaaggt agaggttgcc ggccggtccg ccgttggggc
     1141 cgacgttgcc cttgccgcgc acccggagcc gggagccgtg gtccaccccg ggcggcacgg
     1201 tcacctccag cttttcgcgt cggcgcaccc ggccctggcc gctgcagccg cggcagggct
     1261 tctcgatcat ctggccggtg ccgccgcaga tcgggcaggt gcggctcatg gtgaagaagc
     1321 cctggttgta ggtcacctgg ccgtggccgc cgcactgctt gcagggggcg gctttggtgc
     1381 cggcggccgc tccgctgccc cggcactccg ggcaggcgac caggcggccc acctcgatct
     1441 ccttgcgcac gccctcggcg gcctccggga ggctgatgct taccgaggcc cggaggtcct
     1501 cgccctgcat cacccgccgc ccccggcggc cgccgccgat tccgaaactg ttgaagatgc
     1561 cctccagcag gtcggaattg gcgaagaggt cggagaaatc gtgctggacc cggcccaggt
     1621 cgtcgctcgg gttgaagccg gccccttcca ggccgcggaa gccgaactgg tcgtaggtgg
     1681 cccgtttatt ggcgtcgctg agaacctcgt aggcctcggt cgcctccttg aacttctcct
     1741 cggcggcctt gttgccgggg ttgcggtcgg gatggtactg gagggcctgt ttgcggtagg
     1801 cctccttgat tgactccgga gtggcctggc ggtcgaggcc gagcacctcg taataatctc
     1861 ttttttctgc catgggtgtc atccgggccg ggttcggttg gctgaaccgg ccgggccggg
     1921 ttccatcagg ttaggtgccc ggaccggccg ccgggtttcc gtccggctgc aggtaacggt
     1981 attgcttgcg caggggatcg aaggggcggc cgccgctctc gtagaacttg gtgaagaact
     2041 ccaggtactg gagccggctg gtgtgcacgt aagcgcccag ggtgctgatg aagaggttga
     2101 aggcatggcc gcccaggagc agcagcagga agaagagggt gccgatgacc gggatggcgc
     2161 cggtcatgcc ggccagctgg ttgaccgtta ccgccagcag gccgccggtc aggccgagcg
     2221 cgaagatccg ggcgtaactg agcgtgtcgc ccagcaggtt gccgaccacc agcccgtaaa
     2281 gggtgtaggg ggcgaagaaa atcttgagca ccaggtcgga ctgggtgcgc cactggtagt
     2341 aaatgatgag cagggccgcc actcccagca ccagacccgt taccgggatg gccggctcgg
     2401 cccagggcag ccggccgcgc atgaccagcg tgaagagcag gaagggcagg cccagctgga
     2461 tgaccagcgg cgggatgcct tccagggcgc cgagcgcccc gaagcggcgg atggcctcct
     2521 tggccttgag cagcatgccg gtggttatct ggacgaagcc gactaccagc gaaaagtaga
     2581 agagtttcat ggtgtcggcc gggttgtcca ccggggagaa gagggcgagc cggctctgga
     2641 agcgggccag cggcgccagc ggcgggaaga gggaggtcag gttgccgaac caggagttgg
     2701 tgagcaggcc caccagcatc gtggccagcc ccgagtaaag gaagacgccc aggaggcggc
     2761 gggcggccgg ggccgggaat tttttcatca gcagaaagga gccgagggcc aggagcagac
     2821 cgtaaccgac atcgcccagg cagaaggcga aactgaggat gaagaagaag gccacgaagg
     2881 ccgacgggtc gtagccggag tagacggggc ggccgaagag gtcgaccacc gcttcgaagg
     2941 gctggccgac cgggccgttt tccagggcga ccgggacggc ctcctccggc cgggccggcc
     3001 gggccagcag gccggcctcc gggaaggtcc cggccagaaa tttttcgagg cgcggccggt
     3061 cctgggtccg gatccagccg gtcagcagga gcgtccggcc ggtggcggcc agtttccgct
     3121 tcgcctccag ttccagctgc cgctgccggt agtggtcaag cagggcgagt atctcggagc
     3181 gccgggaccg gaattccgcc gcggccgcct cggcggcccg ccggcggttg cggttgcgtt
     3241 cccgcaggcg ctcgagccgg gccagcgttg cggccggact ggtccggtag gcgggcaggg
     3301 cgaccgtttc ggcccggagg cggttcaggg tttcggagag ttcccgttcc tgccggcggt
     3361 cggcggcgac gaagagcagg gaacgattct tctcctcggc cagcggttgg gcgtggccgc
     3421 cgattttatc cagaccggcc accagttccg ccgtccggtt ccggtccacc cggaccagct
     3481 ggagggcgac ccggccgccg ccgcgcaggg atttcagcgg caggtcgagc caggcgaagg
     3541 gttccagttc ccggccgatc cgttccagcc gttcgtcgcg gccggccagg gccgtcagcc
     3601 gcttttcccg gcggcggacc gcctggaccg tcgcctcgat cgggaaggtc cggagcagtt
     3661 ccggcaggtc cgactcgcgg aggcggaccg gttcggcgcc ggactccagg cccagggcgg
     3721 cggccaggta gttaagccgg ttcagcgttt cgtccgcttc gcggccgggg tcggcctcgg
     3781 ccgggcccag gtcggcctcc tccggctcca gccggccgac ttcgaccagc ccgaaggact
     3841 ccatttcccg gatcagctcc tccttccggg cttcggggag gaagaggtgg acccgctcca
     3901 tcctggcgat ggccatatta tatgcgcttc tttgaaggtg tgcggcggcg ccggcccgcc
     3961 gggccggaaa ccgcgattca gatggaaacc ttaacaaata attctaacac cccggccgcc
     4021 gtttttcaat atatcgggcc ggggcccgga ccgggaggcg gttgccggac gggggcgcca
     4081 gaggttaaaa tatccggacc atggaaaagg cctgcctggc gctggaagac ggactggtgg
     4141 tggagggcgg ttccttcggg gccccgggag aggtcaccgg ggaactggtc ttcaacaccg
     4201 ccatgaccgg ctaccaggag gtgctcaccg atccctccta ccgggggcag ctggtgatga
     4261 tgacctaccc gctgatcggt aactacgggg tcaacgacga ggacgtggag tcggccgggg
     4321 tccaggtcga gggcttcatc gtccgggaga acgcccgcca ttacagcaac tggcgggccc
     4381 gccagtcgct gagcgattac ctgaaggcgg ccggcgtccc cgggattgag agcattgaca
     4441 cccggatgct gaccgttcac atccgggagc agggaagcct gaaggcggtc ctctccaccc
     4501 gggatttcga ccaccagcgg ctggtggcca gagccaaggc ggcccccggc gtggtcgggc
     4561 agaacctggt gatccgggtg agcgcggaga agccctacta ctggaaccgg gagggcgcgg
     4621 gcaaggcggt ggtgctggat tgcggggtca agtacagcat cctgcgctgc gtggccgccg
     4681 ccggctaccg ggtgctggtc ctgccctgcg gggccccggc cgaggccatc ctctccgaac
     4741 ggcccgacct ggtggttctc tccaacggcc cgggcgaccc ggccgccctc ggttcgatcg
     4801 tggccgaggt gcggaaactg ctgggccgcg tcccgatcta cggcgtctgc ctcggccacc
     4861 agatcctggg ccaggcggtg ggcggccgca cctacaagct gaagttcggc caccacggcg
     4921 gcaaccaccc ggtgcgcgac ctgaccggcg gccgggtgat gatcaccgtc cagaaccacg
     4981 gcttctgcgt tgacgccgag accctgccgg ccgggatccg gattacccac ctcaatctga
     5041 acgacgagag cctggagggg atggaaagcc gggaggaacg tttcgcctcg gtccagtttc
     5101 acccggaggc cgggccgggt ccgcacgacg cccgggtctt cttcgagcgc ctccgccagg
     5161 gattctgagc ccggaggccg ttttccgccg gccgcccggc cccttccgcc cttaccggcc
     5221 cagttccgcc ttgatttcag ccagcatctt ctccagccgc cggccgtatt cgatgtagtt
     5281 gcggtagcgg atttcgtcgc tcgaggtcac cgccgcgtcg tggaagacca cggccatgtg
     5341 cggctcgatc gagaagccgc cgtcgtaacc ggtcttgagc aggtcggtca acgtttcgcg
     5401 cacgtaaccc tttccctcgc ccgggaaggt gtagacctcc ttgccgtctt tcaggtagtc
     5461 gtccttgacg tggacgtact cgatgtaggg gcggacgttc cggtagaact cccaggcgtc
     5521 ctgcttgcgg taggggggcc ggccgcgcac gtcgtcgtgg acgggcgggt tgccggtgtc
     5581 gtaaaccagt ttcaatgacg ggctgggaat ggcctccagg aggcgcaggg tgtgctcgta
     5641 ggagagggcg ccccagccgg agcagttttc gtgcaggcag accacgccgc cgtcttcggc
     5701 catccggacg atctcccgga tccgcttgat ggcctcggcc gaccagtccc ggtcgagcac
     5761 ctcgggcggc accgcgaaac tcatgatccg gatgaagcgg gtgcccagct tcttcaggcg
     5821 cgggatggcc tgcttcatct ccgcgtagct gctgtcgggc ggctcggtga tctttttggc
     5881 ccagttggcg atgccgctgc ccaggcagct gaccgtgatc ccggccgcgg ccagcttttc
     5941 ggccaccgct tcgaactcgg cctggccgat cagggtgatg ttttgatcgc caatggcccg
     6001 ggtctcgatg tggcgccagc ccagttccag ggtagcccgg atctgggttt cgatatcctt
     6061 gcccgcctcg tcggcgaaac cggaaaaaaa catgcggcct ccttttttct tagttttttg
     6121 aatgacgacg gaggggcggg aagtccctcc cgcgaaaatc cgcggccgcc tgaaagttta
     6181 tcaggattgg ccggcggcgt caagaacggc cacggacgcg gcggtccgaa cctttctcct
     6241 gctttgacac cgccggtctt ttttgatact atttaggtga attttcctta attgggcgga
     6301 ggcctgttta aaaatgagcg gattcgatcg ttttacaaac caggtgcgcc ggatgctgga
     6361 catggcccgg gacgaggcga tccgtttcgg gcagatcgag atcggcaccg agcatctcgt
     6421 gctggccatg ctgcgcgacg gccagggggt ggcggccgcg gccctcacca gcctgggcta
     6481 cgacctcgat ttcctgcgcc tggagatcga gaaggtcatc gccgtcgggc cggccctgcg
     6541 cattgacgga ccgctgccgc tgagccgccg ggccaagcgg tcgctggaac tgggcgtcga
     6601 ggaggcgcgc cagtacggat ttaactatat cgggaccgag cacatcattc tcgggatcct
     6661 gcgcgagggg gagaaccagg ccagcgcggt gctcggccgg ctcaacctga ccctggaacg
     6721 gagccgcaag gccatctacg agctggtcaa gccgccgctc cagggcggcg agacggccgc
     6781 ccggagcaag accccggccc tggactcctt cggccgggac ctcacccgga tggcccgcga
     6841 cggccggttg gacccggtca tcggccgggc ggcggagatc gagcgcctcg tccacgtctt
     6901 ctgccgtcgc cgcaagaaca acccggtgct ggtgggcgag gccggggtgg gcaagaccgc
     6961 catcgtcgag ggcttcgtcc aggcgatggt ggaggggcgc ctcccggagg cggttgaatc
     7021 gcgccgggtg gtgatgcttg acctggccgc cctggtggcc gggaccaagt accggggcga
     7081 gttcgagcag cgcctcaaga cggtcctgca ggaggtggtg gagaaccagg tggtcatctt
     7141 cgtggacgaa ctgcacaccc tgatcggcgt cggcggggcc gagggggcga tggacgcctc
     7201 ccatatcctc aagccggccc tggccagcgg cgagatccag tgcatcgggg ccaccaccat
     7261 gaacgagtac cggaagcaca tcgaaaagga cgccgccctc gagcggcgtt tccaggcgat
     7321 cttcgtgagc ccgccggaca ccggccagac gatggagatc ctcaaggggc tgcggccgcg
     7381 ttacgccgag caccaccggg tcgagatcag cgacacggcc ctctgggcgg ccgtccagct
     7441 ttcggaccgt tacatcgccg gccgcttcca gccggacaag gccattgacc tgctcgacga
     7501 ggcctgcgcc tcggtccggc tgcgggcggt ccggctgccg gacaacctgc gccggatgga
     7561 agcccagctc aaggccctga ccgagaagcg cgagaaggcg acccgcgatc tggattacga
     7621 gacggccatc agcctgcgcg agcaggagaa gaccgagcgg gtcggtttcg acctgctcaa
     7681 gaaacgctgg cgcctgtcaa tttcacccac cgagggcgtg gtgaccgagc aggacatttc
     7741 cgaggtggtc agccgctgga ccggcgtgcc ggtccggcag atctccgagg acgagggggc
     7801 ccgcctgctt cacatggagg aggagctcca gcgccaggtc atcggccagt ccgaggccgt
     7861 ctcggccgtc tgccgggcaa tccgccggtc gcgcaccggc atcaaggacc ggcgccggcc
     7921 ggtcgggtcc ttcctcttcc tggggccgac cggggtgggc aaaaccctgc tggcccgggt
     7981 gctggccggc ttcctctttg atgaccagga ggccctgatc cagatagaca tgagcgagta
     8041 catggagcgt ttctcggtct cccgcctgat cggcgccccg cccgggtacg tgggctacga
     8101 ggagggcggc cagctgaccg agcgggtgcg ccgtcgcccc tactcggtca tcctcttcga
     8161 cgagttcgag aaggcccatc cggacgtcta caacctgctg cttcagatcc tggaggacgg
     8221 ccacctgacc gacagtttcg gccgccgggt ggacttccgc aacaccatcc tgattctgac
     8281 ctcgaacctc ggcaccggtt cgcttcagga gaccgcttcg gtcggcttcg tcagcgacgg
     8341 cggccagccg gccgattacg agcagacccg gcagctgctc aagaacgagg ccgaacgggt
     8401 cttccggccc gagtttctta accggcttga cgaaatggtg gtcttccgca gtttgaccga
     8461 ccgcgacgcg gccgccatcg tgcggctgga ggtcgaccgg gtcctggtcc ggctccggga
     8521 gcagggactg gggatcagcc tttcggagtc agccttccgc ttcctggagc gggagggttt
     8581 tgatccgaag ctgggcgccc ggccattgcg gcgggccatt acccgccacc tggaggaccg
     8641 cctctccgag gagatgcttt ccggccggct gcggaccggc ggccgggttc gggtaggcat
     8701 ggagcgcagc ggccgccgtc tctccttcga tatcgagcac ccggccgccg aaacgatcgg
     8761 gacagcccat tgaggcgccc cggccgcggc cggcgttgga gccagggacc gttttccggc
     8821 ccggttaaaa catggcggat ttgatgatca aggtcgcggt ggtgggcgcg ggacacatgg
     8881 gcagcggaca tatccgggcc taccgtgcct gcgccggggc ccggctccgc tatgtctgcg
     8941 agcccgagga ggcgcgtggc cgtagcgcgg ccgaggcggc cggcgccggc tgggccgccg
     9001 attaccggca ggtcatcgac caggtggacg cggtcagcgt ggcggttccc accaaccttc
     9061 acttccgggt ggcctccgat ttcctggccg ccggcaaggc cgtcctgctg gagaagccga
     9121 tcgccgaccg gcttgaggat gccgaggccc tggtccggat gagccgggag cggggcgcgg
     9181 tcctgcaggt gggtcatatc gagcgcttca acccggccgt ccgggaactg cgccgccggc
     9241 tccgggatcc gcgctacctg gagattcacc ggctgggcac cttcaagggg cggggtgtcg
     9301 aggtgagcgt cgtctccgac ctgatgatcc acgacatcga cctggcgctg gacctggtcg
     9361 accggccgct gctggaggtg agcgccctgg gcatggccgc cttcaccgcc actccggaca
     9421 tcgccaccgg ccgcctggtc ttcgagggcg gccgggtggc caacctcacc gccagccgca
     9481 tcagcgacaa gagcctgcgg aaggtgcgcg tcttcgagat ggactgttac tggtcgctgg
     9541 actgcgagca ccgggaactg gtctgctacc ggaaacggcc cgagtccgac tggcgcggca
     9601 aggcgcggcc gaccctggcc gacctgatca gccgggaagt cctggccgtc gaggcggccg
     9661 atccgctcca gcgcgagctt gaatctttct tgaccgcggt ccgggagggc ggccagccgg
     9721 aggtgaccgg cgaagacggc cggcgcgccc tcgaggtcgc gttgcagata gagaagtcga
     9781 tcgcaaacaa ccagccggtt tcgtccggat tctcctaacc ggggctgttt cccggattga
     9841 aagaaaggat ggtgcagaat aagatgatcg agcatgccag ccgggaagcc cgctttcccc
     9901 acgatttcac cgccaagcac attttcctca ccaagggcgt cggccgcgac aaggagaaac
     9961 tgaccagttt cgagcaggcc ctgcgcaacg ccggcatcgc gcccttcaac ctggtccaca
    10021 tcagcagcat ctttccgccc ggcgcccggc tgatcacccg ggagcggggt ttgaagatgc
    10081 tcaagcacgg ccagatactc ttctgcgtca tgagtcggaa cgagaccaac gagccgcacc
    10141 gcctgatcac cgcctcgctg ggtctggcca tacccaagaa taccgagaag tacggctatc
    10201 tctcggaatt ttccgggttc gggatgaccg acgagcgttc gggcgattat gccgaggacc
    10261 tggcggccac tatgctggcg accatcctgg gggtcaagtt cgacccggac agcagttacg
    10321 acgagaagaa gcaggtctgg cggctgagcc gggagatcgt gcgcaccacc aacgtcaccc
    10381 agtcggccgt cggtgacaag aacggccgct ggacgacggt ggtgacggcc gccgtctttg
    10441 tactctgagc cgccgttttc gacggggaga ccggagatgg agatacccta cacgtttgcc
    10501 ggctggccgc cccggcgcct ggaggaggcg cgggcggcca tcctgccggt ggcttatgac
    10561 gccaccacct cttaccggtc cgggacgcgt ttcggacccc aggagatcat cctggcctcg
    10621 cgtttcctgg agatgtacga cgaggccctc ggtctggagc ccggcggtcc gggcgtggtc
    10681 accctgcccg aactctggcc ggacctggcc tcgccggagg cggccctgcg ggaggtccag
    10741 gcttacgtgg acggactgct ggagaagaac cttttcccgg tggtcctggg cggagagcat
    10801 tccatcacct acggcgcggt ccaggctgtc cggaagttcc atcccggcct ctcggtgctg
    10861 gtgctggacg cccacgccga cctgcgccag gattaccagg gcacccgcta caaccatgcc
    10921 tgcgtcagcc ggcgcatcct ggacctgggc gtgccggtga ctgtgctggg cgtccgctcg
    10981 ctgccgagcg aggacctgcc gctgctggag tcggggaagg agccggggct gaacctggcc
    11041 tgggcctcgc agctgaaggc ggagccggat tggctgggcc gtttcgggga ccgcctgacc
    11101 gagacggttt acctttcgat cgacctggat ttcttcgacc cggcttacgt accggcggtc
    11161 ggcacgcccg agcccggcgg cctcggctgg gatgagacgg ccgccttcct ggccggcctg
    11221 gccggccgcc gccggttggt cggtttcgac gtcgtggaac tggacggttc ggttcaccac
    11281 accccctcag cctatctcgc cgcccggctc gtccaccggc tgctgggact ctgcctgccg
    11341 gcggccgacc ggtattgaca gtaggggagc ggtcattgta gaatacaagg aaaatcaagg
    11401 taaatacccc ggttgtctcc tttcccgaac cgatcgaaca ggaggaagtc aagatggctg
    11461 atttgcaagc gattgccaac ggcgtgatca gcggcaaggc ggcggatgtc aagaacctgg
    11521 ttgaggccgc gatcaaggaa ggcgtagggg cgggcgatat tctcaagaag ggcctgattg
    11581 ccggtatgga cgtggtgggc gtcaagttcc gcgccaacga attttacgtg ccggaggtcc
    11641 tgatcgccgc ccgcgccatg aacgccggca tggaagtgct ccagcccgag ctggtcaaga
    11701 gcggcgtcaa ggccatcggc aaggtcggag tgggaacagt caagggcgac ctgcacgata
    11761 tcggcaagaa cctggtggtg atgatgctca agggggccgg tttcgaggtg gttgacctgg
    11821 gcatcgacgt tccggaagat aagttcgtcc aggcggtcag ggatggggtc caggtgcttg
    11881 gcctttcggc cctgctgacc accaccatgc cggcgatgaa atcggtcatc gaggcgatca
    11941 agacggctgg cctgcgcgac caggtgaaga tcatgatcgg cggggcgccg gttacccagg
    12001 cctacgccag cgaggtgggc gccgacgctt acggcgagga tgccgccacc gccgccatca
    12061 aggccaagga gctgctcggc cttaattgac gggcaaggtt tggacctgtc aaatatcggt
    12121 ttgatcgcgg gcgcttacct gctgggggcg atacccttcg ggtacctggc cggccggctg
    12181 gccggagtcg atgtccggaa agcgggcagc ggcaacatcg gcgccaccaa tgtcctgcgt
    12241 accgtcggcc gcggtccggc ggccgccacc ctcctcttgg atatcctcaa gggtttcctg
    12301 cccgtctggc tggcccggcg cccctgggcg ttgccaccgg cctgggttgc ggcgatcgcc
    12361 ctggcggtgg tggccggcca cgtcttctcc ccctttctga agttccgcgg gggcaagggt
    12421 gtggccaccg cggtcggagt cctgctcggc atctcgccgc cggcggcgct ggccgccgtg
    12481 gccgtcttcg gattggtctt cggcctcggc cggattgttt cactcgcctc cctggccgcc
    12541 gccgtcctgc tcccgttcct gctcggactc ctggctcccg aacccgccta cgcgattttc
    12601 ggcggcctgg tcggagcgct ggtggtcctg cgccaccggg ccaacctcga tcgcctgctt
    12661 tccagatcgg aaccccgtac ccaatggaaa aaatagccgt cgtgggcgcc ggcgcctggg
    12721 ggaccaccct ggcgctgctg ctggccgggc cggaccggtc ggtcctgctc tgggaaaagt
    12781 ttcccgatta tgcccgtcag ctggccgggg agcgagagaa cgtcaagttc ctgcccggct
    12841 tcgaactgcc ggagaacctg gacgtgaccg acgatctggc cttggccgcg tcggccccgg
    12901 cggtggtgct ggtcactccg tccttcgcct gccgggagac ggccggccgg ctggccgccg
    12961 ccggctgccg gggtccggtc ctggtggcca ccaagggcct ggaggcgggc agcggcctgc
    13021 gtatgagcca ggtggtcgcc gaggcgatgc cgggtgtggc ctggtcggtt ctctccggcc
    13081 cgaccattgc tcgcgaagta gcggccggtc ggccgaccgc cgcggtctgt gccggaccgg
    13141 acgaaaagct gaattttttc ttccaggatc tcttcagtac cggccatttc cggctctaca
    13201 ccagcgccga tgccgccggc gtcgagttcg gcggcgccct caagaacccg ctggcgatcg
    13261 ccgccgggat cgtcgaggga ctggggctgg gggataacgc caaggccacg ctgctctgcc
    13321 gggggctggc cgagatggcc cggctcggaa cggccctggg cggacaggaa cgcaccttct
    13381 tcggactttc gggactgggt gacctgatta ccaccgcctg cagtcctcaa tcccgcaacc
    13441 accgtttcgg ggtggccatc ggcggcggcc agccgatcga agcgtatctc aaggggcagg
    13501 aatcggtggt cgaagggtac cacagtgccc ggccggccct taagctggcc gccggggcgg
    13561 gcgtggagat gccaatcgtc agccaggtgg cggcggtcct cttcgagggc aagccgccgg
    13621 cggcggcggt ccgcgaattg atgggccggc cgctcaaggc cgagtgaggg accggatggc
    13681 ggagcaggcg gaacggggcg gggagaaggt ttactaccgg atcggcgagg tcagccggcg
    13741 gatcggcgtg gccgcgcaca ccatccgtta ctgggagaag gagttccggc tcggccggcc
    13801 gcgccggaac agccggggtc accgcgtcta taccggggcg gaagtggcga aactgctccg
    13861 gctcaaggag ctgctttacg ggcgcggcct gcgcatcagc gccgctaagc gcgtcctgcg
    13921 cgagtccggt tcgaagtccc gccaggcggc ggccgaccag cggccgctgc gccggctggc
    13981 gcaggagttg cggcggctgg agaagaatct ggagcagttg gcccggctcg aggccgagga
    14041 ttgattgtgg cggctggtca gtttttctgc tgctgaagcg tgtagaggta gtaatagcgt
    14101 ccccgccgtt ccatcaactg cgcgtgcgtc ccctcctcgg caatccgccc cttctccatc
    14161 acgatgatcc aatcgctctt ctgcagggtg accagccggt gggcgataat caggcaggtg
    14221 cggtcggcca tcaccttttc gagcgcctcc tggaccaacc gctccgattc cgagtccagg
    14281 ttgctggtcg cctcgtccag gatcagcacc ggtgaaccgg cca
//
LOCUS       DAOQYN010000019        13898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_4879_length_13898_cov_4.425847, whole genome shotgun sequence.
ACCESSION   DAOQYN010000019 DAOQYN010000000
VERSION     DAOQYN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4879_length_13898_cov_4.425847"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="PKN80_01600"
     CDS             <1..1193
                     /locus_tag="PKN80_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34741.1"
                     /translation="GRERPAVIFGAGNIGRGFLGQLFFNAGYRTCFVEARPELAALLN
                     ERGAYPLWIVSDAETRRYRISNLAALPVGSIDRVAAALADADLAATAVGVNNLARVAP
                     GLAAGIALRAERGRPDPLNIIICENLLAAAGLLAAEIRRHLPPACIPYFQERVGLVET
                     VVSRMVPPVPADLLAREPLLVLVEPYQVLPVARQAFKGDRLPEVAGFLPVDDIHAYEE
                     QKLYIHNLGHAVCAYYGYLKGYRYIWEAVRDPEIRDRLDRVLAEAGGALTRKHPFLSG
                     QLEGYIADLRERFGNRALGDTVFRVGREPLRKLGPNDRLIGALRLCLEQQVEFEDLAR
                     AVAAALAYDYPEDPEAVELAGLIAAGGPGRVLHEVCGLKPDSPVYRAVETAYVRLAAA
                     RPGK"
     gene            1190..2215
                     /locus_tag="PKN80_01605"
     CDS             1190..2215
                     /locus_tag="PKN80_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ34742.1"
                     /translation="MTGRERYFRSIRGQAVDHPARVPILMEFAARYIGSHYGAFASDY
                     RVLVEANLRCAADFGFDQVSVISDPYRETAGYGAEIVFIRDGVPRCPRPPLAVDRDPA
                     RLKRPDPRRSPRLLDRIRAVETFRERAGDYSILGWVEGPAAEAADLRGVTNFLSDLAL
                     EPEYAARLMDECLETAVEFARAQLAAGADTIGVGEAIVSQVSPRMYEDLIKPRQQRLV
                     DEIRKAGGLVRLHICGDITRHLPSLAGLDVDVLDLDWMVDLAGSRRLLGPRTALAGNL
                     DPAEAVLRSTPDRIAADLRRCYQASGNPYLVGAGCEIPAATPFENLRALCRPIPWRPN
                     PEQADRD"
     gene            2353..3396
                     /locus_tag="PKN80_01610"
     CDS             2353..3396
                     /locus_tag="PKN80_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HNQ34743.1"
                     /translation="MKAALLRGIEDLVYQDRPVPEPGPGELLVKVRACAVCGTDVKIY
                     HHGHRLIRFPRVTGHEIAGEVAGAGAGVSEFRVGDRVQLAAAVPCGECFFCRRGAPAM
                     CDRLIAVGYHFDGGFAEYLKVPERMLRNACVNRIPDGLPFEEAALAEPLACVINGQER
                     SGIGFGSTVLVLGAGPIGCFHAQLARRRGAFRVIVSDPDPARLEAARFTGADRYVNPG
                     SEDPAAAVREINGGRLADQVMVATGAGIAQEQSLQLVDKRGTVNFFGGLPKGQTVNLD
                     TNLIHYGELRVVGTHGSAPSHNAQALALLAGGSIPGRPYLSAVFPLADIGSALAAAEA
                     RQGLKVIVRPTES"
     gene            3396..4385
                     /locus_tag="PKN80_01615"
     CDS             3396..4385
                     /locus_tag="PKN80_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ34744.1"
                     /translation="MKKINKSEARFLLKQMMEIRKFEEAVMDLLARGIADGGSHLYAG
                     EEAVAVGAMAAIRTDDYITSTHRGHGHAIAKGGSLPHLMAEILGKQTGVCKGKGGSLH
                     LADVSQGNLGANGVVGGSLGIAAGAGLSIRLRATDQVVLCFFGDGATNTGLFHEALNM
                     TGRWSLPVIFICENNLYGMSVSVERACAVTDLTQKAVPYRMPAECLDGMDVLAVREAV
                     ARRVAAARKGGGPAFLVANTYRYYGHSRSDPRVYRTREEEKNWKDRDCIEQFAARLVK
                     AGILTQAEVDECDRETAAAIAAAVEFAIQSPDPAPEELYRDVYYEAEEGSIYA"
     gene            4378..5352
                     /locus_tag="PKN80_01620"
     CDS             4378..5352
                     /locus_tag="PKN80_01620"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNQ34745.1"
                     /translation="MREITYRQALNEALAEEMDRDPSVFLMGEDIAIYGGAYGVTAGL
                     MQRFGEERVRDTAISEAAIIGAGLGAAITGLRPVVEIMYVDFMGICLEQVNNQVAKIR
                     YMFGGKTRVPLVIRTEGGAGRTLGAHHSQSLEAWFLHIPGLKVVMPSTPADAKGLLKS
                     AIREENPILFIEHKMLYNTKGPVPEGEYLVPIGRAEVKRPGRDVTIVAHSRMLLFALE
                     AAERLAGEGIEAEVIDPRTLLPLDIETIAASVRRTHRAVLVEEGTRTGGTGAEIGLQI
                     MEHCFDYLDAPIRRIAGADVPMPKSPVLEKLTIPDPDRIYREVRELLA"
     gene            5368..6546
                     /locus_tag="PKN80_01625"
     CDS             5368..6546
                     /locus_tag="PKN80_01625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNQ34746.1"
                     /translation="MVKEIIMPKLGETMEEGFLVKWLKSEGDRVAKGEVIFEVMSDKT
                     NFEVESPRAGILRRILVAAGDRPIPVTTVVGYLADNLEEPLPAGSVVAPAPKTGGAEP
                     LPAAGAEGPVSGRKDDAPVAASPLARRLARELGLPLASIPGTGPGGRVTERDVRSAAE
                     VGLKKGPPAPAASGKKRLSPMRQLIAARLSQSKREIPHYYLRRVLDVSALTAARVRLK
                     ASSPEITYTDLLVRAVAAALGEFPDLNATFENDSVTVYETVNLGLAVSVAEGLVVPVI
                     KSAGKLSLAELVAERKRLVALARANRLQRGDLEGGTFIISNLGMYGVDSFYPIIYPPQ
                     VAILGVGAIREGLLVRDGSIMIRPLLELTLAADHRVVDGACGGRFLQRIQELIDQDGE
                     "
     gene            6548..7222
                     /gene="cysE"
                     /locus_tag="PKN80_01630"
     CDS             6548..7222
                     /gene="cysE"
                     /locus_tag="PKN80_01630"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNQ34747.1"
                     /translation="MLQGVREDIRSVFARDPAARSLVEVLLCYPGLHAVWLHRVAHFF
                     WRTGSRTFARLISHFNRFLTGIEIHPGARIGRRFFIDHGMGVVIGETTEVGDDVLLYQ
                     GVVLGGTSLVKKKRHPTLGNRVVVGTGAAVLGPINIGDGVLIGAGSVVIKNVPEGVTV
                     VGIPARVVGEHRKLPIDLEHGKLPDPFAEPLRLLFRECSRLHERVQSLEAELARLKQE
                     ETRTEK"
     gene            7257..8066
                     /locus_tag="PKN80_01635"
     CDS             7257..8066
                     /locus_tag="PKN80_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNQ34748.1"
                     /translation="MLPWRIRVLAGFVAFNLLASAAAAGVYRVKSGDTLGSISRRYST
                     TVENLKSLNNLSGNTIHVGQRLKVPGEVSQPESSSSTYYTVKSGDTLAGISRRVGVSL
                     ANLKNWNNLKSSRIYAGQKLRLKPVAGGGGSAPRGYATRKYYYTVRKGDSLSIIARRT
                     HTTVARLRSLNRLKKNSVIRPGQRLVVSVRYLRPEARGGTGPDTPPEIDPKPIIEGEL
                     VFHTVKEGETVESIAQQYGLSADDIRSANLMPGEAPVRKGQILAIPSAQQP"
     gene            8080..9129
                     /gene="mtnA"
                     /locus_tag="PKN80_01640"
     CDS             8080..9129
                     /gene="mtnA"
                     /locus_tag="PKN80_01640"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNQ34749.1"
                     /translation="MSGEFFTLKWTGRTLTILDQTRLPAETAYLNLGTVSEVWDAIRE
                     LKVRGAPALGVTAAYGLYLGVRENGPQDWSGFLEELHRVKVYLERSRPTAVNLSAALD
                     RIEVCCQAMSSNTRPEEGLKFLLNEAGRLEAEDQLFCERIAAFGQGIIPARARILTHC
                     NAGALATAGIGTALGIIGRARASGKDVEVFADETRPLLQGSRLTAWELQRRGVRVTLI
                     CDSMAAWTMKERGINLVLVGADRIVANGDTANKIGTYGLALLAEQHRIPFYVAAPVTS
                     FDLKTANGRQIPIEERPPAEITSCGGRPVAAAGVRVYNPAFDVTPGKLVSAFITDRGI
                     IRPPYDLESFRRAFV"
     gene            9172..9960
                     /locus_tag="PKN80_01645"
     CDS             9172..9960
                     /locus_tag="PKN80_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNQ34750.1"
                     /translation="MKKNPTAEFRLARLSYGQVKKRRFETAVLPWGATEPHGLHLPHG
                     TDYLKVEKIAEAACDRAAARGARVLLLPSVPYGVNTNNLGFPFAMSIQPTTILAILND
                     LLHSLSVHGIRKVVIVNGHGGNEFKPLLRELFGRHGLFTCVVNYWALAPEVAEKVFKD
                     RRGDHANEMETSLVLVYYPELVDMKAAGPGRMRQTGIEALEKGWAVFPRPWHVFTLDS
                     GAGDPRAASAEKGRKFLAPVIERLADFLVELSARPYSKDFPYRR"
     gene            10173..11162
                     /gene="trpS"
                     /locus_tag="PKN80_01650"
     CDS             10173..11162
                     /gene="trpS"
                     /locus_tag="PKN80_01650"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNQ34751.1"
                     /translation="MSKKRILSGMRPTGKLHLGHLAGALSNWIRFQEEYDCFYEIADL
                     HALTTDYQNPSGIRDNIREMLLDFLAFGLDPEKATIFIQSKVPEISEMHLLLSMITPL
                     GWLERCPTYKEQLAQLADREITNYGFLGYPVLQTADIISFKAVRVPVGEDQVPHLELA
                     REIVRRFNFLYGELFPEPEAILTVNPKLLGTDGRKMSKSYGNAILLADTPEEIRQKTA
                     TMFTDPLRPYRKDPGHPDACPVFGLLQMYRPEESAAVRPACTSAQLGCSDCKKKLTEI
                     LLEFLSPYQRKREELAREPGRLQEILDRGNRRAREIVNDVLAEAKTKMGLYGY"
     gene            11152..12258
                     /gene="hisC"
                     /locus_tag="PKN80_01655"
     CDS             11152..12258
                     /gene="hisC"
                     /locus_tag="PKN80_01655"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNQ34752.1"
                     /translation="MDIKTMTRAGLAGLAAYRPGKPIATLLKEKGIESAIKLASNENP
                     LGASPLAVEAARAAAVDLNRYPEGSGAAVRDRLAGLYGISADEIILGSGSSEVLALAL
                     SAFVDPGEEVVLPTPSFSIYGTLVLAAGGTPVPVRLEPDFSYNLKEFEKRLSERTKAV
                     ILCSPNNPTGTIVRKADLRAFAAGLPRRTLLVLDEAYAEYAESPDFESGLTFFRERPL
                     VVARTFSKLYALAALRIGYAVSGRVIIEEMNKIRPPFNTTVPAQAAAAAVLDDRKFIA
                     ASLKNNREGKEFLGRALAGLKLDFTPSEANFILVDFQGGAPEICARLEDLGVIVRPLD
                     NPELRARYLRVTVGTPAENRRFIEGLRTVLGPAG"
     gene            12265..13008
                     /gene="cdaA"
                     /locus_tag="PKN80_01660"
     CDS             12265..13008
                     /gene="cdaA"
                     /locus_tag="PKN80_01660"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNQ34753.1"
                     /translation="MPEFTLLDLLDITLLSVLCYYIFLTFRGTRGVQLLKALVVIFLI
                     TFLSQVLGLPSLQWVLEKVLAFGTLALLIIFQPEIRNALVRIGGPVFFNNPARISAEN
                     VEEIIEAVFQLAREKKGALVVLEGEVGLAEYLASGKPVDSELSKELLLCLFHPASPLH
                     DGAVIISQNRIAGAGCFLPLSDRTDFAPSVGSRHRAALGLSEETDSLVVIVSEESGLV
                     ASAHGGRLEERLDPVRLRRLIQETFKVKP"
     gene            13005..13196
                     /locus_tag="PKN80_01665"
     CDS             13005..13196
                     /locus_tag="PKN80_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34754.1"
                     /translation="MKKESPRRHRVLAALVNNPGLKILALLAAIFIWLYVHNIAIFIP
                     SRGITLRPIYQRFVNPNRR"
     gene            13201..>13898
                     /locus_tag="PKN80_01670"
     CDS             13201..>13898
                     /locus_tag="PKN80_01670"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNQ34755.1"
                     /translation="MKLGVNIDHVATLRQQRRGSEPDPVAAARLAEAAGADSIVCHLR
                     EDRRHIQERDLIRLRREIGTRLNLEMAASDEIISIALREKPDWVTLVPERRQELTTEG
                     GLDVVGQAADLKGVIGRFRDAGIPASLFIDADPVQIDAAARLGAWAVELHTGRYADAA
                     GEPEREAERLRMVEGAARAARAGLKVAAGHGLDRFNLAPIARIPEIEELNIGYSIIGR
                     AVFIGLEAAVAEIIR"
BASE COUNT         2323 a         4591 c         4625 g         2359 t
ORIGIN      
        1 cgggccggga gcggccggcg gtgatcttcg gcgccggcaa catcggccgg gggtttctcg
       61 gtcagctctt cttcaacgcc ggttaccgga cctgtttcgt tgaggcccgg ccggaactgg
      121 ccgcgcttct taacgagcgc ggcgcctacc cgctctggat cgtctccgac gccgagacgc
      181 gccgttaccg gatttccaac ctggccgccc tgccggtcgg ttcgattgac cgggtggccg
      241 cggcgctggc cgatgcggac ctggccgcca cggcggtcgg ggtcaacaac ctggcccggg
      301 tggcgcccgg cctggcggcc ggaatcgcgc tccgggccga gcgcggccgc cccgacccgc
      361 tcaacatcat catctgcgaa aacctgctgg cggcggccgg gctcctggct gccgaaatcc
      421 gccgtcacct gccgcccgcc tgcattcctt attttcaaga acgggtcggc ctggtcgaga
      481 cggtggtcag ccgcatggtc ccgccggtgc cggccgacct gctggcccgg gagccgctgc
      541 tggtcctggt cgagccctac caggttctgc cggtggcccg ccaggccttc aagggcgacc
      601 ggctgccgga ggtggccggt ttcctgccgg tcgacgacat ccacgcctat gaggaacaga
      661 aactttacat ccacaacctt ggccacgccg tctgcgccta ctacggatac ctgaaggggt
      721 accgttacat ctgggaagcg gtccgcgacc cggagatccg ggaccggctg gaccgggttc
      781 tggccgaggc gggcggcgcg cttacccgga agcatccttt cctttccggt cagctggaag
      841 gctatatcgc cgacctgcgg gagcgttttg gaaaccgggc cctgggtgat accgtcttcc
      901 gggtgggccg cgaaccgctg cgcaagctgg gtccgaacga ccggctgatc ggcgcgcttc
      961 gcctctgcct ggaacagcag gtggaatttg aagacctggc gcgggcggtg gcggcggccc
     1021 ttgcctacga ttacccggag gatccggagg cggtcgagct ggccggactc attgccgccg
     1081 gcggcccggg gcgggtgctg cacgaggtgt gcggcctgaa gccggactcg ccggtttacc
     1141 gggccgtgga aaccgcttac gtccgcctgg cggcggcgcg gccgggaaaa tgaccggacg
     1201 ggaacgttat tttcggagca ttcgcggcca ggccgtggat cacccggccc gggtgccgat
     1261 cctgatggaa ttcgccgccc gttacatcgg cagccattac ggggccttcg cctccgatta
     1321 ccgggttctg gtcgaagcca acctgcgctg cgccgccgac ttcggattcg accaggtcag
     1381 cgttatctcc gacccctacc gggagacggc cggttacggc gccgagattg tttttatacg
     1441 cgacggggtg ccccggtgtc cgcggccccc cctggccgtc gaccgggacc cggcccggct
     1501 caagcggccc gatccgcgcc gttctccgcg tctgctggat cggattcggg cggtcgagac
     1561 cttccgggag cgggccggcg attactccat cctgggctgg gtcgaggggc cggccgccga
     1621 ggcggccgac ctgcggggcg tcaccaattt tctctccgac ctggctctgg agccggagta
     1681 cgccgcccgg ctcatggacg aatgccttga aacggcggtg gagttcgccc gggcccagct
     1741 ggcggccggc gccgacacca tcggcgtcgg cgaggcgatt gtcagccagg tctcgccgcg
     1801 catgtacgag gacctgatca agccgcgcca gcagaggctg gtggacgaaa tacgcaaagc
     1861 cggcgggctg gttcgcctcc acatctgcgg agacatcacc cgccacctgc cgtcgctggc
     1921 cggccttgac gttgacgtgc tggatctcga ctggatggtg gacctggccg ggagccggcg
     1981 gctactcggt ccccggaccg ccctggccgg caacctggac ccggccgaag cggttctgcg
     2041 ctccacgccg gaccggatcg cggccgacct ccggcgttgt taccaggcct ccggaaaccc
     2101 ttacctggtc ggcgccggct gcgaaatacc ggcggccacg ccgttcgaga acctgcgggc
     2161 cctctgccgg cccattccct ggcggccgaa tccggagcag gccgatcggg attaacggcc
     2221 gaattgacgc tgtgctataa tcattaattg ccggccggag ataacgttaa tgccggtttg
     2281 agacggtttt aaacatgacc ggcggccggt ttgagagtca tcccggcggc cggggggaga
     2341 aaactggtga agatgaaagc ggccctgctg cggggcattg aggaccttgt ttatcaggat
     2401 aggccggtgc cggaaccggg gccgggcgaa ctgctggtga aggtgcgcgc ctgcgccgtt
     2461 tgcggtaccg acgtcaagat ctatcaccat ggtcaccgtc tcatccggtt tccgcgcgtg
     2521 accggccacg agattgccgg ggaggtggcc ggcgccggcg ccggcgtcag cgagttccgg
     2581 gtcggcgacc gggtccagct ggcggccgcc gttccctgcg gcgagtgttt cttctgccgg
     2641 cgcggggcgc cggcgatgtg cgaccggctg atcgccgtcg gctaccactt cgacggcggt
     2701 ttcgccgagt acctcaaggt tccggaacgc atgctccgca acgcctgcgt caaccggatc
     2761 ccggacgggc ttcccttcga agaggccgcc ctggccgagc cgttggcctg cgtcatcaac
     2821 ggccaggagc gaagcgggat cggtttcggc tcgacggtgc tggtgctggg cgccgggccg
     2881 atcggctgtt ttcatgccca gctggcccgc cggcgcggcg ccttccgggt gatcgtttcc
     2941 gatcccgacc cggcccgcct ggaagcggcg cgtttcaccg gcgccgaccg ctacgtcaac
     3001 cccggttccg aggatccggc cgccgcggtg cgggagataa acggcggccg gctggccgac
     3061 caggtcatgg tggccaccgg cgccgggatc gcccaggagc agtcactcca gctggttgac
     3121 aagcggggca cggtaaattt cttcgggggc ctgcccaagg gacagaccgt gaacctggac
     3181 accaacctga ttcactacgg cgagctccgg gtggtcggca cgcacggttc ggctccttct
     3241 cacaacgcgc aggcactcgc cctgctggcc ggaggctcca tcccgggccg cccttatctg
     3301 agtgccgttt ttcccctggc cgacatcggt tcggccctgg cggccgccga ggcgcgccag
     3361 gggctgaagg tgattgtccg gcccacggag tcgtgatgaa gaagatcaac aaaagcgagg
     3421 cccggtttct cctcaagcag atgatggaga tccgcaaatt cgaagaggcg gtgatggacc
     3481 tgctggcccg gggaatcgcc gacggcgggt cgcaccttta cgcgggagag gaggccgtgg
     3541 cggtgggagc catggccgcc atccggaccg acgactacat caccagcacc caccgcggcc
     3601 acggccacgc catcgccaag ggcggttcgc tgccccacct gatggccgag atcctgggca
     3661 agcagaccgg cgtctgcaag ggcaagggcg gttcgcttca cctggccgac gtctcccagg
     3721 gaaacctggg cgccaacggg gtggtgggcg gcagtctcgg gattgccgcc ggggccggtc
     3781 tttcgatccg gctgcgggcc accgaccagg tggtcctctg cttcttcggc gacggcgcca
     3841 ccaacaccgg gctcttccat gaagcattga acatgaccgg ccgctggagc ctgccggtca
     3901 tcttcatctg cgaaaacaac ctttacggca tgtcggtttc ggtggaacgg gcctgcgcgg
     3961 tcaccgacct tacccagaag gcggttcctt accggatgcc ggccgagtgc ctggacggca
     4021 tggacgtgct ggcggtccgg gaggcggtcg cccggcgggt ggcggccgcc cggaagggcg
     4081 gcggcccggc cttcctggtc gccaacacct accggtacta tggccacagc cggagcgacc
     4141 cgcgcgtcta ccgcaccagg gaagaggaaa agaactggaa ggaccgcgac tgtatcgaac
     4201 agtttgccgc ccggctcgtc aaggccggca tcctcaccca ggccgaggtg gacgagtgcg
     4261 accgggagac cgccgccgcc atcgcggccg ccgtggaatt cgccatccag agcccggacc
     4321 cggcccccga ggagctttac cgcgacgttt actacgaggc ggaggagggc agcatctatg
     4381 cgtgagataa cctaccggca ggcgttgaac gaggccctcg ccgaggagat ggaccgggat
     4441 ccttccgttt ttctgatggg cgaggacatc gccatttacg gcggcgccta cggggtgacc
     4501 gccggcctga tgcagcgatt cggcgaagag cgggtccgcg acaccgccat ctccgaggcg
     4561 gccatcatcg gcgccggact gggtgcggcc atcaccggtc tgcggccggt ggtggagatc
     4621 atgtacgtcg atttcatggg catctgcctg gagcaggtca acaaccaggt ggccaagatc
     4681 cgctacatgt tcggcggcaa gaccagggtg ccgctggtca tccggaccga gggcggcgcc
     4741 ggccggaccc tgggcgccca ccattcccag agcctggagg cctggttcct gcacataccg
     4801 ggcttgaagg tcgtgatgcc gtccaccccg gccgacgcca aggggctgct caagtccgcc
     4861 atccgggagg agaaccccat tcttttcata gagcacaaga tgctttacaa caccaagggg
     4921 ccggttcccg agggcgagta tctggttccg atcggccggg ccgaggtgaa gcggccgggc
     4981 cgcgacgtga ccatcgtcgc ccattcccgg atgctgctct tcgccctcga ggcggccgaa
     5041 cgcctggccg gcgagggcat cgaggccgag gtgatcgatc cccggaccct gcttccgctg
     5101 gacatcgaaa cgatcgccgc ctcggtgcgc cggacgcacc gggccgtcct cgtggaggag
     5161 ggaacccgaa ccggcggcac cggcgccgag atcggcctgc agattatgga gcattgcttt
     5221 gattacctgg acgcgccgat ccgccgcatc gccggcgccg acgtcccgat gcccaagagc
     5281 ccggtcctgg aaaaactgac cattcccgat ccggatcgaa tctatcgcga agtcagggaa
     5341 ctgctggcct gaggggctga aaattatatg gtcaaagaaa tcatcatgcc caagctgggc
     5401 gagacgatgg aagaggggtt cctggtaaag tggctcaagt ccgagggcga ccgggtcgcc
     5461 aagggcgagg tgattttcga ggtgatgagc gacaagacca acttcgaggt tgaatcgccc
     5521 cgggccggca tcctgcgccg gatcctggtg gcggccggcg accggccgat tccggtgacc
     5581 acggtggtcg gctacctggc cgacaatctt gaggaaccgc tgccggccgg ctcggttgtt
     5641 gcgccggcgc caaaaaccgg gggagcggag ccgcttccgg cggccggagc cgaggggccg
     5701 gtctccggac ggaaggatga tgctccggtg gcggcttcgc cgctggcccg gcgcctggcc
     5761 cgggagctcg gccttcccct ggcctcgatt cccggcaccg ggccgggcgg tcgggtgacc
     5821 gagcgtgatg tccggtcggc cgccgaggtc ggcctgaaga agggtccgcc ggcgcccgcg
     5881 gcctccggta aaaagcgcct ttcgccgatg cgccagctga tcgccgcccg cctgagccag
     5941 agcaagcgtg agatccccca ttactacctg cgccgggtcc tcgacgtttc ggccctgacc
     6001 gcagcccgcg tccgcctgaa ggcctccagt ccggagatca cctacaccga cctgctggtg
     6061 agggcggtcg ccgcggcgct gggcgagttt cccgacctga acgccacctt tgagaacgat
     6121 tcggtgaccg tctacgaaac ggtcaacctc ggcctggcgg tttcggtggc cgaggggctg
     6181 gtggtgccgg tgatcaagtc ggccggcaag ctttccctgg ccgagctggt cgccgaacgg
     6241 aagcggctgg tggcgctggc ccgggccaac cggctccagc gcggcgacct ggagggggga
     6301 accttcatca tctccaacct gggcatgtac ggagtggatt ccttttaccc gatcatctat
     6361 ccgccccagg tggccatcct tggcgtcggc gccatccggg agggcctgct ggtccgggac
     6421 ggctccatca tgatccggcc gctcctggaa ttgacgctgg ccgccgatca ccgcgtggtg
     6481 gacggcgcct gcggaggccg tttcctccag cgcatccagg aattgatcga ccaggacggg
     6541 gagtgaaatg ctgcagggag taagggagga tatccggagc gtctttgccc gtgatccggc
     6601 cgcccgcagc ctggtcgagg tcctgctctg ctatccgggc ctgcacgcgg tctggctgca
     6661 ccgggttgcc catttcttct ggcgcaccgg ttcccgcact ttcgcccggc ttatttctca
     6721 ttttaatcgt tttttgaccg gcatcgagat tcatcccggt gcccggatcg gccgccgttt
     6781 cttcattgat cacggcatgg gcgtggtcat cggcgagacg accgaggtcg gcgatgacgt
     6841 gcttctctac cagggcgtgg ttctgggcgg gacctccctg gtcaagaaga agcgccaccc
     6901 gaccctcgga aaccgggtgg tggtcggcac cggcgcggcc gtgctcggtc ccatcaatat
     6961 cggggacggg gtgctcatcg gggccggttc ggtggttatc aagaacgtgc ccgaaggggt
     7021 tacggtggtc ggtatcccgg cccgggtggt cggcgaacac cggaaactgc cgatcgacct
     7081 ggaacacggg aagctgcccg acccgtttgc cgagccgctg cgcctgctct tccgggaatg
     7141 ctcgaggctt cacgagcgcg tgcagagcct ggaggcggaa ctggcccggc tgaagcagga
     7201 agagacgaga accgaaaaat gaacccggcc cgggccgggg atcgaaagga ggcgggatgc
     7261 tgccatggag aataagagtc ctcgccggct ttgtggcatt caatcttctg gcttccgcgg
     7321 ccgcggccgg cgtttaccgg gttaagtcgg gcgacaccct gggcagcatc agccgccgct
     7381 actcgaccac ggtcgagaat ctcaagtccc tcaacaacct gtccggaaac accatccacg
     7441 tcggccagcg cctgaaagta cccggtgaag tctcccagcc ggagagttcc tcttccacct
     7501 actataccgt caaatcgggc gataccctgg cgggcatctc ccgccgcgtc ggagtgagtc
     7561 tggccaacct gaagaattgg aacaacctga aatcgagccg catctacgcc ggccaaaaac
     7621 ttcgcctgaa gccggttgcc ggcggcggcg ggtcggctcc gcgcggctac gccacccgca
     7681 agtactacta cacggtgcgc aagggggaca gcctttcgat catcgcccgg cggacccaca
     7741 ccaccgtcgc ccggctgcgg agtctgaacc ggctcaagaa gaacagcgtc atccgcccgg
     7801 gccagcgcct ggtggtttcg gtccgttacc tgcgcccgga ggcccggggc gggaccgggc
     7861 cggacacccc gccggaaatc gatcccaagc cgatcatcga gggcgaactg gtcttccata
     7921 ccgtcaagga gggcgagacg gtcgaatcga tcgcccagca atacggactc tcggccgacg
     7981 atatccgttc ggccaacctg atgcccggcg aggccccggt ccggaagggc cagatcctgg
     8041 ccattccgtc ggcccagcag ccctgaaggt gacaagagca tgagcggcga attctttacc
     8101 ctgaagtgga ccggccggac actgaccatc ctggatcaga cccgcctgcc ggccgagacg
     8161 gcctacctga acctgggtac cgtcagcgag gtttgggatg cgatccggga actgaaagtc
     8221 cggggggcgc cggcgctggg tgtaacggcc gcctacggtc tttacctggg ggtgcgggaa
     8281 aacgggccgc aggactggtc cggcttcctc gaggaactgc atcgggtcaa ggtctacctt
     8341 gagcgcagcc ggcccaccgc cgtgaacctt tccgccgccc tggaccggat cgaggtctgc
     8401 tgccaggcca tgtcgtcgaa tacccgtccc gaggaagggc tcaagttcct cctgaacgag
     8461 gccggccggc tcgaggccga ggaccagctt ttctgtgaaa ggattgccgc cttcggccag
     8521 gggatcattc cggcccgggc ccgtattctc acccattgca acgccggcgc cctggccacc
     8581 gccgggatcg gcaccgccct ggggatcatc ggccgggccc gggcctccgg aaaggacgtc
     8641 gaagtttttg ccgacgagac ccggccgctg ctgcagggaa gccgcctgac cgcctgggag
     8701 cttcagcgcc ggggggtccg ggtaaccctg atctgcgaca gcatggccgc ctggaccatg
     8761 aaggaacggg gcatcaacct cgtcctggtg ggggccgacc ggattgtcgc caatggagat
     8821 accgccaaca agatcggcac ttacggcctg gccttgctgg ccgaacagca ccggataccc
     8881 ttttacgtgg cggcgccggt caccagcttc gatctgaaaa cggccaacgg acgccagatc
     8941 ccgattgagg aacggccgcc ggcggagatc acctcgtgcg gcggccggcc ggtggcggcg
     9001 gccggggtcc gggtttacaa cccggccttt gacgtgaccc cggggaaact ggtgagcgct
     9061 tttattacag acagagggat catccggccg ccttacgacc tggaatcctt ccgccgggcc
     9121 ttcgtttgac ggccggccgg atccctcact tctttctgcc tggaggcaag catgaagaaa
     9181 aacccgaccg ccgagtttcg cctggcccgg ctgagttacg gccaggtcaa aaaacgccgc
     9241 tttgaaaccg ccgttctgcc ttggggggcg accgagccgc acggcctgca cctgcctcac
     9301 ggcactgatt acctgaaggt cgaaaaaatc gccgaggcgg cctgcgaccg ggcggccgcc
     9361 cggggtgccc gggtgctcct gcttccgtcc gttccctacg gggtcaacac caacaacctc
     9421 ggttttccat ttgcgatgag catccagccg accaccatcc tggccatcct gaacgacctg
     9481 ctccattcgc tttccgtgca cgggatccgc aaggtggtca tagtcaacgg ccacggcggc
     9541 aacgaattca agccgctgct gcgcgaactc ttcggccgcc acggcctctt tacctgcgtg
     9601 gtgaattact gggccctggc cccggaggtc gcagagaagg ttttcaagga ccgccgcggc
     9661 gaccacgcca acgagatgga gaccagcctg gtgctggtct actacccgga actggtggac
     9721 atgaaggccg ccgggccggg ccggatgcgc cagaccggca tcgaggctct ggagaagggc
     9781 tgggcggtct ttccgcgccc ctggcacgtc ttcaccctgg acagcggcgc cggcgacccc
     9841 cgggccgcga gcgccgagaa ggggcgcaag ttcctggcgc cggtcatcga acggctggcc
     9901 gactttctgg ttgaactctc cgcccggcct tactcaaagg atttccctta ccggcgctga
     9961 gcccggcccc ggttggaaat cgcctggaag ggtctttcgc ctccggccgg cgttcctttt
    10021 tcgatcctcg aaggccggcg cttcggttta catcccggcc gtgctgcgtt tataatatta
    10081 aaatccataa acatcaaagg cggccggggt ttgttatcct ttgacgccgg cgcgtttttc
    10141 acgccagcca ccgccacgat actgaaggac cgatgagcaa aaaacgcatt ctgagcggca
    10201 tgcggccgac cggtaaactc cacctgggcc acctggcggg cgccctgagc aactggatcc
    10261 gattccagga agagtacgac tgcttttacg agatcgccga cctgcacgcc ctgacgaccg
    10321 attaccagaa cccgtcgggc atccgggata acatccgcga aatgctcctc gatttcctgg
    10381 ccttcggact cgacccggag aaggcaacca tctttatcca gtccaaggtt ccggaaatat
    10441 cggagatgca cctgttgctt tcgatgatca ccccgctggg ctggctggaa cggtgtccga
    10501 cctacaagga gcagctggcc cagctggccg accgggagat taccaactac ggttttctgg
    10561 gttacccggt gctccagacg gccgacatca tctccttcaa ggcggtccgg gttccggtgg
    10621 gcgaggacca ggtcccgcac ctggagctgg cccgggagat cgtgcggcgc ttcaacttcc
    10681 tttacggtga actcttcccg gaaccggagg cgatcctgac ggtcaacccc aagctgctgg
    10741 gcaccgacgg ccggaagatg agcaagtcgt acgggaacgc catcctgctg gccgacacgc
    10801 ccgaggagat ccggcagaag accgcgacca tgttcaccga tccgctccgg ccctaccgga
    10861 aggatcccgg ccacccggac gcctgcccgg tcttcgggct gctccagatg taccggccgg
    10921 aggagagtgc cgcggtccgg ccggcctgta cctcggccca gctgggctgt tccgactgca
    10981 agaagaaatt gaccgagata ctgctggagt tcctgtctcc ctaccagcgc aagcgcgagg
    11041 agctggcccg ggagccgggc cggcttcagg aaatactgga tcgcggcaac cggcgggccc
    11101 gggagatagt caacgacgtc ctggccgagg ccaagacgaa gatggggctt tatggatatt
    11161 aagacgatga cacgagccgg cctggccgga ttggcggcct atcggccggg aaaaccgatc
    11221 gcgacgcttc tgaaggagaa gggcatcgag agcgccatca agctggcctc caacgagaac
    11281 ccgctgggcg cttcacccct ggcggtcgag gcggcccggg cggccgccgt cgatctgaac
    11341 cgttacccgg aaggcagcgg cgcggcggtc cgggaccggc tggccggact ttacgggata
    11401 tccgccgacg agatcatcct gggcagcggg agcagcgaag tgctggccct ggccctttcg
    11461 gcctttgtcg atccgggcga agaagtggtg ctgccgacgc cctccttctc catttacggg
    11521 acgctggtcc tggccgccgg cggcacgccg gtgccggtaa gactggagcc ggatttcagc
    11581 tacaacctga aggagttcga aaagcggctt tcggagcgga ccaaggcggt catcctctgt
    11641 tcgcccaaca acccgaccgg caccatcgtg cgcaaggccg acctgcgcgc cttcgccgcc
    11701 ggcctgccgc ggcggacgct tctggtcctg gacgaggctt acgccgagta tgccgaaagt
    11761 cccgatttcg agagcggcct gaccttcttc cgggaacggc ccctggtggt cgcccgcacc
    11821 ttctccaagc tatacgctct ggccgccctg cgcatcggtt acgcggtctc cggccgggtc
    11881 atcatcgagg agatgaacaa gatacgccct cccttcaaca ccaccgtgcc ggcccaggcg
    11941 gcggccgcgg cggtcctgga cgatcggaag ttcatcgcgg cctccctgaa gaacaaccgg
    12001 gaaggcaagg agtttctcgg ccgggccctg gccgggctca aacttgattt cacgccgagc
    12061 gaggccaact tcatcctggt tgattttcag ggcggcgccc cggaaatctg cgcccggctg
    12121 gaggatctcg gcgtgatcgt cagacccctg gacaacccgg aactgcgcgc ccgctacctc
    12181 cgggtgacgg tgggaacgcc ggccgagaac cgccggttca tcgaaggact gcggaccgtg
    12241 ctcggaccgg ccggctaaag ggaaatgccg gaattcacgc tgctcgacct gctggacatc
    12301 acgctgcttt cggtcctctg ctactatatt ttcctgacct tccgggggac ccgcggcgtc
    12361 cagctgctca aggccctggt ggtcatcttc ctgatcacct tcctctccca ggttctcggc
    12421 ctgccctcgc tgcagtgggt gctggagaaa gtcctggcct tcgggaccct ggccctgctg
    12481 atcatcttcc agcccgagat ccggaatgcc ctggtgcgca tcggcggtcc ggtcttcttc
    12541 aacaatccgg cccggatcag cgccgagaac gtcgaggaaa tcattgaggc ggtctttcaa
    12601 ctggcccgtg aaaaaaaagg cgccctggtc gtcctggaag gggaggtggg actggccgaa
    12661 tacctcgcct cgggcaagcc ggtggacagt gagctgagca aggaactcct cctctgtctt
    12721 tttcacccgg ccagtcccct ccatgacggg gcggtcatca tctcccagaa ccggatcgcc
    12781 ggcgccggct gcttcctgcc tctttcggac cggaccgatt ttgccccctc ggtgggcagc
    12841 cgccaccggg ccgcgctcgg actttcggag gagaccgaca gcctggtggt gatcgtctcc
    12901 gaggagagcg gcctggtcgc ctcggcccac ggcggccggc ttgaggaacg gctcgatccg
    12961 gtgcggctgc gccgcctgat ccaggagacc ttcaaggtga aaccatgaag aaagaatcgc
    13021 cccggaggca ccgtgttctg gccgcactcg tcaacaaccc cggcctgaaa atcctggccc
    13081 tgctggcggc gattttcatc tggctttacg tgcacaatat cgccatcttc attcccagcc
    13141 gcgggattac cctgaggccg atttaccagc gtttcgtgaa tccgaaccgg aggtgagcgc
    13201 atgaagctgg gcgttaacat tgatcacgtg gccaccttgc gtcagcagcg ccggggcagc
    13261 gaaccggatc cggtggcggc cgcccggctg gcggaggccg ccggcgccga cagcatcgtc
    13321 tgccacctgc gcgaagatcg tcgccacatc caggagcggg acctgatccg gctgcggcgc
    13381 gagatcggga cgcgtctcaa cctcgaaatg gccgccagcg atgagataat ctcgatcgcc
    13441 cttcgcgaaa agccggactg ggtgacgctg gttccggaac ggcgccagga attgacgacc
    13501 gagggcggcc tggatgtcgt cggccaggcg gcggacttga agggagtgat cggccgtttc
    13561 cgggacgcgg gaattccggc cagcctcttc atcgatgccg atcccgttca gattgatgcc
    13621 gcggcccgtc tcggcgcctg ggcggtcgag ctgcacaccg gccgttacgc cgacgcggcc
    13681 ggggaaccgg aacgggaggc cgagcggctg cggatggtcg aaggggcggc ccgggccgcc
    13741 cgggccggcc tgaaggtggc cgccggtcac gggcttgacc ggttcaacct ggcgccgatc
    13801 gcccggatcc cggagatcga ggaactgaac atcggctatt cgattatcgg gcgagcggtc
    13861 ttcatcggcc tggaggcggc ggtggccgag atcatccg
//
LOCUS       DAOQYN010000020        13733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_4952_length_13733_cov_4.509724, whole genome shotgun sequence.
ACCESSION   DAOQYN010000020 DAOQYN010000000
VERSION     DAOQYN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13733
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4952_length_13733_cov_4.509724"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKN80_01675"
     CDS             complement(<1..194)
                     /locus_tag="PKN80_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34756.1"
                     /translation="MKKQLTSRQRVMTAFEHREPDRIPAWCGSSPGFWEKARRGLHLD
                     DEGIRQRFGDDFRRIFESYST"
     gene            390..1526
                     /gene="hemW"
                     /locus_tag="PKN80_01680"
     CDS             390..1526
                     /gene="hemW"
                     /locus_tag="PKN80_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNQ34757.1"
                     /translation="MKNSRRRRSGLYIQVPFCRSRCPYCHFYSTTDLDLIPAWLEAVR
                     IEAARYQGRFSVFDTLYLGGGTPSVLEAADLHRLVEGVRAVFFFASGAEWTIEVNPDD
                     LTPGKLKTYRRLGFNRISLGLQSFHDRELRLLGRRYGAETARRSFRMARESGFENINL
                     DLICAFRGQCLKDWRLTLKETVELQPEHVSTYLLETDLSWNRPGISPLGESEQESFYL
                     EAAERLANSGYLHYEVSNFARKRGWRSRHNLKYWNGVDYLGLGPDAHSFQSGRRWWNA
                     ADLPAYCRHLRLGRDPVAGLEELTSEERRRENIYLGLRTSRGLSLAGLPGVPEEILSG
                     GLATIARGRLCLTERGFLLAEFVSGLLWDRLPLAGNPRQPAGFQ"
     gene            1528..3735
                     /locus_tag="PKN80_01685"
     CDS             1528..3735
                     /locus_tag="PKN80_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNQ34758.1"
                     /translation="MNGRTILFKAILFLCLAAGTAAGDGPPAAAGRLRVGLVLGGGGA
                     RGLAHIGVLKELERCRVPIDCIAGTSMGAVVGGLYAAGLSAGEIESEMLRIDWADLFS
                     DRPSRRNLSFQKKSDARNYLEAEIGMKAGRIRLPPGLVAGQKLDLLLTTLLINAAGTD
                     DFEHLPIPFQCLATDIASGETVLLKEGRLMPAIRASMSVPGVFAPVELNNRLLVDGGL
                     VNNLPVEAVQAMGCDLVIAVDVGYPLLDKENLRDMLDIVRQVMTLNQIENTRRQVERL
                     TPSDLLIKPELDGFSGSGFNRVSELVARGESAVRAAEPRMLAYRIDEEAYRRERDRIK
                     AFREQPVHLDFVEVDSPARIAPEVIRARLKTQSGEPLNLARLRGDVSRVYELGFFDKV
                     DFEIASRNGRRGLLLHTDEKRRGYDYLSFGLSFIDDFQGGAGYDFLAGLTRTALNPRG
                     GEWRTRLRLGSNTELGTSFYQPIDYGGIFYLLPRVDYRRRALNYYLAGHNQANYRIST
                     LEAGLEAGFQWEKYGIFSVAAERGWLEVEPAIGYGPAAVDRERTIFRYRLAFDQLDSA
                     NFPRHGGSFDLQLSQFRKLWGGDDSYDRLELATTAALSRSRNTWLLYARGGEALESRL
                     PYYADFTLGGLFSLSGYPIDSLRGNAYLLGGLIWFREVGRVPFLTGLPLFAGFGLEAG
                     NAWAERSRVALDDLKGSGLVFLGADTYFGPLFLAGGFAESREGVLYLYLGRRF"
     gene            3767..4357
                     /locus_tag="PKN80_01690"
     CDS             3767..4357
                     /locus_tag="PKN80_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34759.1"
                     /translation="MGIGVWLEELRLWCAGRRWTTRLPILVWMSYLLVRHLADPEYQG
                     LIKPLNLGIHELGHFVFMPFGEFLSVLGGSLFQLLVPVISIFMFWRQADFFAIAFALG
                     WLGTNFFDIATYIGDAQAMALPLVSPFGGEGAIHDWNYLLTRLDRLEACRGIAGAVRF
                     TGVFFLLAGVAAGGWLLWWMFRQRRSEPVEGGFEEL"
     gene            complement(4371..5117)
                     /locus_tag="PKN80_01695"
     CDS             complement(4371..5117)
                     /locus_tag="PKN80_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HNQ34760.1"
                     /translation="MAPNAFPTAFLYGAVSGLALVLGAGTALRFRLRQRATARMMAFG
                     SGILICALTLGLLEEAFHYGGAAAVTIGFLAGGLAFIGGDYLIHRAGGRQHRRKPWLK
                     NERCASGQAILLGAFLDGLPESAALGITIFSGQGRGPLMLAAIFLSNFPEALASVAGL
                     RRTGFRPARILKLWGLAGISTLAVTLLSFLFLPGLPVRWLGLIEAFAAGAILAMLANS
                     MMPEAYEEGGLSIGIMTVLGCLVAFLLARF"
     gene            complement(5121..5993)
                     /locus_tag="PKN80_01700"
     CDS             complement(5121..5993)
                     /locus_tag="PKN80_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNQ34761.1"
                     /translation="MRGSGSSQAAKRAYWTREMETGYEFMMKVLAHPVCDAGEALVSL
                     PEALRGTGIEVVFFRRQRIPGHRPIFRLRAGLVKPWLSAAEAMNRHGWRIRIEDGYRT
                     RSMQNPLNRPPALFDAILRRLLWETEGRFPSPEFVFRRLTVLVTTIPKLGTHMSGSAM
                     DISVLKRKNGEEVDRGAPYLEMSELTPMASPYAPAAARRNRAGISRIMAEHGFAAYPF
                     EFWHYSGGDAYAAILSEKKQPARYGPVDLLNDSGRIRPIDNPALPLINPVEIQAALSK
                     AARRLGIKIPAKME"
     gene            6091..7317
                     /locus_tag="PKN80_01705"
     CDS             6091..7317
                     /locus_tag="PKN80_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ34762.1"
                     /translation="MAGIKNQVVVVGGGPAGIMAAIRAAERGRRVLLLERTGELGKKL
                     LISGGGRCNLTNRRGLDEFLAAYHNGAFLRNAFARFFNQELMDFFESGGIKLTVERGQ
                     RVFPASQSSAEIVAFLRRRLEAARVQLWFRNRLIRANPLPSGWELVTRQDTFSSETLL
                     LATGGRSYPETGSTGDGYNIARRLGHTINKPRAALCGLELKEKWIRDWQGVSLENVAL
                     TLSQGDRELGSEFGEAIFTHYGLSGPAVLNLSRILTGREHQAVTRLSIDFKPALDAGK
                     LDQRLQRELNLGRRQVKNILRTLLPMRLVERFLSVAGLPAEKPAHQVTAEERRRLLEQ
                     LKAFPLTVAALRPADEAIVTRGGVEVKEIDPRRLESRLHPGLFFAGELIDVDGPTGGF
                     NLQAAFTTGYVAGSHL"
     gene            complement(7370..9202)
                     /locus_tag="PKN80_01710"
     CDS             complement(7370..9202)
                     /locus_tag="PKN80_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ34763.1"
                     /translation="MHFGRMDRMNRLPNEENPEARLEIGKLVVYLRPHLLQVAATLLI
                     SVTITLLHLAPPRLIGSIIDQAVARKALSELYRNATLLLTVYICAHVLSGLKGFLIGR
                     LGQRVIYDLWQDVYRNLQRLSFNFYDRNQTGSIMSRITNDVSAVERVIVDGIDTVIIA
                     VMTLLGITVILFRLNWRLALVSLIPIPILLVLAWLVTQKAHLIYREVRRKMGEISALL
                     QDSISGIREIKSFVREDYEVNRLAEKSSDYFSTNLQAIRLWSFFSPSILTTTSIGTFL
                     VLLFGARQAVVTGDMSAGEIVSFLFYLGLFYQPIHQLNWFNHMLQHARAASERIFEII
                     DAVPQILEASNAVTLPRPVRGEVVFHDVSFSYQPDQEVLHAVNFEARPGELVALVGAT
                     GAGKTTIVSLIPRFYDADRGLILIDGIDVKKLKLHDLRENIGIVMQEPFLFNGTVMEN
                     IAYGRLEATFEEIVAAAKLANADQFINALPDGYAHQIGERGIKLSVGEKQRLAIARAL
                     LKNPPILILDEATSSVDNQTEALIQQAIDHLLRDRTSFVIAHRLSTVMHADKILVIDG
                     GCIVESGTHDSLLAAGGVYARLYRIQWRHFRSQESEGMKAEIGG"
     gene            9419..10003
                     /locus_tag="PKN80_01715"
     CDS             9419..10003
                     /locus_tag="PKN80_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNQ34764.1"
                     /translation="MYRVLLIGDSIRMGYEPFVRRELEGLAEVLGPEKNGGDSDNVLR
                     NLPEWLETFPADLVHLNCGLHDIKRPFGSSSNGIPRFQYADNLRQIFSHLVAHKIPVI
                     WATSTPVDEKLHHQNKSFDRFEADLEHYNATALEACREFGLEVDDLFGLIERAGKSAY
                     LLDDGVHFKPEGYQLLGREVARVIRAALEKLRLK"
     gene            10000..10530
                     /locus_tag="PKN80_01720"
     CDS             10000..10530
                     /locus_tag="PKN80_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HNQ34765.1"
                     /translation="MKKSELPGYRKLARVYLERAGIIITPAEARSIEVADFGLGDFER
                     TGLLLLVYVSTDRYCAKELVLLPGQVCPEHRHPPRSGKDPGKQETFRCRWGRVELFLP
                     GRPAAVFSEPRPAAFTVGRRVLLKPGLQYTIPANTRHWFRAGSKGAVVSEFSSANFDA
                     GDIFTDRRVRRLAGEK"
     gene            10527..11567
                     /locus_tag="PKN80_01725"
     CDS             10527..11567
                     /locus_tag="PKN80_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNQ34766.1"
                     /translation="MSPTRVAERRRKRAGICCGGNWLLDFVKIIDAYPAEERLANILE
                     ESVGNGGAPYNVLVDLARIDPDLPLEGVGVIGRDENSRRIMADIRRYRIRPVGIFRTD
                     RLPTAYTDVMTDRKTGRRTFFHNRGANRLLGPEHFDFKRIRAEILHVGYALLLDGLDA
                     SDPRHGTGMARVFHSARAAGLKTSLDVVSDQDRARFRQVVSPALPEVDYLILNEFEAG
                     ALSGLELDPKRPREGFAAAARRLFDAGVRELVIIHMAVGAWLFSRKGEAFFQPAHQLP
                     AGFIRSTVGAGDAFCAGILYGLVKGLDQAGCMKFANAAAALSLSAATASDGLPRRRRI
                     EAFARRTPCLNL"
     gene            11668..12099
                     /locus_tag="PKN80_01730"
     CDS             11668..12099
                     /locus_tag="PKN80_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ34767.1"
                     /translation="MLFGKKQVEAGGVGYRIVAADDQEDVRTILQKGLTAAGHTVFSA
                     VDGEDALEKIKNEKPDLVILDIRMPKLDGIHALQVIKKSPLTRNLPVIMLTGESSDEQ
                     LLAGYMFGADYYITKPFRLQTVLTGITMVMKPDKRPGKYTI"
     gene            complement(12195..>13733)
                     /locus_tag="PKN80_01735"
     CDS             complement(12195..>13733)
                     /locus_tag="PKN80_01735"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNQ34768.1"
                     /translation="SNPDEDRMLAQSISESRNLFLAAYFRKIEEKPGRLPAGEDPVFP
                     LAQFRKEPGNLAIINAPPDPDGVTRGMPLFVESGGKSYPTLGMALACAYLDIKPETIQ
                     ILGNRLEITDRSGRKLMLPVDRRGRLRINFSGGLGTFQGCPWETLLTSHIQSQSGRKP
                     DFPLETLKGKIVLIGSAVSGGGDLRPTPVDPVFPMFALHAGVLQDILSGSFLVRPGAP
                     ARLLILILLGLLAGLATDQVRLGRRALMLTAILAGYLAVAWGLFRSDLWLDLAAPILA
                     VFLTFAAETNRNYFAERREKTFLRQTFTRYLAPEIVSQLLTARASLNLGGDRRRVTVL
                     FADLRGFTRLSEGRPPEEVVRLLNEFLGLVTEAVFRNRGTLDKFIGDCAMAVFGAPLD
                     LDEPELAAARTALEIQAGLAELASRWQTELGIGVGVGIGINTGEAVVGNIGSRRRLDY
                     TAIGDAVNVAQRLESIAGPGVILLSEATFQALAGKIRAEAQEPVQVKNRVQPVIPYRL
                     IAVE"
BASE COUNT         2490 a         4201 c         4413 g         2629 t
ORIGIN      
        1 gtcgaatagg attcgaagat ccggcggaaa tcgtccccga aacgctggcg gattccttcg
       61 tcatcgaggt gcagaccccg gcgggccttt tcccagaacc cgggcgagga gccgcaccag
      121 gccggaatcc ggtccggttc ccggtgttcg aaagcggtca ttacccgctg gcgcgaagtg
      181 agttgctttt tcaagtccgg cctccttttt tgctctctcg ggaatcaagc cgcctaattt
      241 ataacaccgg cggaaagaac ccgtcaagaa aggggagaac gaatttgcgg cggcggcttc
      301 cgttatggta ttaataattt ttcaacccgg cgctcagccg cgttcgcggt tggccgcccg
      361 gctttttttg aggatcgccg ccttgaagca tgaagaatag ccggcgccgg cgttcgggac
      421 tttacatcca ggtgcctttc tgccggtccc gctgccctta ctgtcatttt tattcgacca
      481 ccgatctgga tctgattccg gcctggctgg aggcggtccg aatcgaagcc gcccgatacc
      541 aaggccgctt ctccgttttt gatactcttt acctgggcgg cggaacccct tcggttctcg
      601 aagcggcgga cctgcaccgc ctggtcgaag gcgtgcgcgc cgtttttttc ttcgcgtccg
      661 gcgccgagtg gacgatcgag gttaacccgg atgacctcac ccccgggaaa ttaaaaacct
      721 accgccgact gggtttcaac cggatcagcc tcggcctcca gtcgtttcat gaccgggaac
      781 tgcgtttgct ggggcgtcgt tacggagccg aaaccgcccg gcgttccttc cggatggcca
      841 gagagtccgg cttcgagaat atcaatctgg atttgatctg cgccttccgg gggcagtgtt
      901 tgaaggactg gcggcttacc ctcaaggaga cggttgaact tcagcctgag catgtctcca
      961 cctatctcct ggagacggat ctgtcctgga accgacccgg aatcagcccg ctcggggaat
     1021 cggaacagga aagtttttat ctcgaggccg ccgaacggct ggcgaattcc ggttacctcc
     1081 actacgaggt ctccaacttc gcccgaaaac ggggctggcg ttcacgccat aaccttaagt
     1141 actggaatgg cgttgattac ctcggactgg ggccggacgc gcactccttc cagtccggcc
     1201 ggcgctggtg gaacgcggcc gatcttccgg cctattgccg gcaccttcgc ttgggccggg
     1261 acccggtcgc cgggctggaa gaactcacct cggaagagcg gcgccgggaa aatatttacc
     1321 tgggacttcg aaccagccgc ggcctttcgc tggccgggct gcccggggtg ccggaggaga
     1381 ttctctccgg cggcctggcc acgatagcgc ggggccggct gtgcctgacc gaacgcggtt
     1441 tcctcctggc ggaattcgtt tccggcctgc tctgggacag gctgccgttg gcggggaacc
     1501 cgcggcaacc ggccggattc cagtaaaatg aacggacgaa cgattttatt taaggccatt
     1561 ctcttcttat gcctggccgc cggaacggcc gccggagatg gaccgccggc cgccgccggc
     1621 cgtctcaggg tcggcctggt cctgggcggc ggcggcgccc ggggcctggc ccacatcggg
     1681 gtgcttaagg aattggagcg gtgccgggtt ccgatcgact gcatcgccgg gaccagcatg
     1741 ggcgcggttg tcggcggact ttacgccgcc ggcctgtcgg ccggcgagat cgagtccgag
     1801 atgctgcgta tcgattgggc ggaccttttt tccgaccggc cttcccgccg caacctgagt
     1861 ttccagaaaa agagcgatgc ccgcaattac cttgaggccg agatcggcat gaaggccggc
     1921 cgcatccggc tgccgccggg gctggtggcc gggcagaaac tggatttgtt gctgacgacc
     1981 ctgctgatca atgcggccgg aactgacgat ttcgaacatc tgccgatccc ctttcaatgc
     2041 ctggctaccg acattgccag cggtgaaacg gtgctcctga aggaaggacg attgatgccc
     2101 gccatccggg ccagcatgtc ggttcccggg gtcttcgccc cggtggaatt aaacaaccgg
     2161 ctgctggtgg acggcggcct ggtcaacaac ctcccggtcg aggcggttca ggcgatggga
     2221 tgcgacctgg tgatcgcggt cgatgtcggc taccccctgc tggataagga aaacctgcgc
     2281 gacatgcttg atattgtccg ccaggtgatg accctgaacc agattgagaa tacccgccgc
     2341 caggtcgagc ggctcacccc gtctgacctc ctgatcaaac cagaactgga cggcttcagc
     2401 ggcagcggtt tcaaccgggt ttcggaactg gtcgcccgcg gcgaatcggc cgttcgggcg
     2461 gcggaaccgc gaatgctggc ctaccggatt gatgaagagg cctatcgccg ggaacgggat
     2521 cggatcaagg cctttcgcga gcagccggtg cacctggatt tcgtcgaggt ggattccccc
     2581 gcccgcatcg cgcccgaggt gatccgggcc cgtctcaaga cgcagtcggg cgaacccttg
     2641 aacctggccc gccttcgcgg cgacgtttcc agggtttacg agctggggtt cttcgacaag
     2701 gtggattttg aaatcgcgtc ccgcaatggg aggcgcggac tgctcctgca caccgacgag
     2761 aaacgccggg gatatgacta cctcagtttt ggcctgagct tcatcgacga tttccagggc
     2821 ggcgccggtt acgattttct ggccggactc acccggaccg ccctcaatcc ccggggcggc
     2881 gagtggcgca cccggctgcg gctgggaagc aacaccgagc tgggcacctc tttttaccag
     2941 ccgatcgatt atggcgggat cttctacctg cttccccggg ttgattaccg gcggcgcgcc
     3001 cttaactatt acctggccgg ccataaccag gccaattacc ggatttccac cctggaagcc
     3061 ggattggagg ccggcttcca atgggagaag tatggaatct tttcggtggc ggccgaacgg
     3121 ggctggctgg aggtcgagcc ggcgatcggc tacggtccgg ccgccgtgga ccgggaaagg
     3181 accattttcc gttaccggct ggccttcgac cagctggaca gcgccaattt cccacgccat
     3241 gggggaagtt tcgaccttca gctcagtcag ttccgcaagc tgtggggagg ggatgattcc
     3301 tatgaccgtc tggaactggc aaccacggcc gccttaagcc gttcccggaa cacctggctc
     3361 ctgtacgccc ggggcggcga agccctcgaa agccggcttc cttactacgc cgattttacc
     3421 ctgggcggac tcttttcgct ttccggttac ccgatcgatt ccctgcgcgg caacgcttac
     3481 ctgctgggtg ggttgatctg gtttcgcgaa gtgggacggg ttcccttcct gaccgggctg
     3541 cccctctttg cgggcttcgg gttggaagcc ggcaacgcct gggcggaacg cagccgggtc
     3601 gccctggatg acctgaaggg cagcggcctg gtttttctgg gggccgacac ctatttcggg
     3661 ccgctcttcc tggccggcgg tttcgccgag tcccgggagg gtgttctcta tctttatctc
     3721 ggccgccgtt tttgagattc gcctttcaaa gttcaggggg aagccaatgg gaatcggagt
     3781 ctggcttgaa gagttgcgac tctggtgtgc cggccgccgc tggacgaccc ggctgcccat
     3841 tctggtttgg atgtcttacc tgctggtgcg ccacctggcc gatccggaat accagggttt
     3901 gatcaagccg cttaacctgg gaatccacga actgggccat ttcgtcttca tgcctttcgg
     3961 cgaattcctt tccgtgctgg gtgggtctct ttttcaactc ctggtgccgg tcatttcgat
     4021 cttcatgttc tggcgccagg ccgatttttt cgccatcgcc ttcgccctgg gctggctcgg
     4081 caccaatttt ttcgatatcg ccacctacat cggtgacgcc caggccatgg ccctgccgct
     4141 ggtctccccc ttcggcgggg agggggccat tcacgattgg aactatcttc tgaccaggct
     4201 ggaccggctc gaggcctgcc gcgggatagc cggggcggtc cgtttcacgg gggtcttctt
     4261 cctgctggcc ggcgtggccg cgggcggctg gctgctctgg tggatgttcc ggcagcgccg
     4321 atccgaaccg gtcgagggcg gcttcgagga gctatgattt tttcgccggc tcaaaatcgg
     4381 gccagcagga aggcgaccag gcaacccagc acggtcatga tcccgatgct caatccgcct
     4441 tcttcgtaag cctcgggcat catgctgttg gccagcatgg ccaggatcgc cccggccgcg
     4501 aaggcttcaa tcaatccgag ccagcggacc ggcaggccgg gcaggaacag gaagctcagc
     4561 agggtgaccg ccagggtgga aattccggcc agcccccata gttttagaat ccgcgccggc
     4621 cggaatcccg tccgacgcaa gccggcgacg gaggcgagcg cttcggggaa gttggaaaga
     4681 aagatggccg ccagcatcaa ggggccgcgc ccctgtccgc tgaagatggt tattcccagc
     4741 gccgccgatt ccggcaatcc gtccaggaag gcgcccagaa gaatggcctg gccgctcgcg
     4801 cagcgttcgt ttttaagcca gggttttctc cggtgctgcc ggccaccggc ccggtgaatc
     4861 aggtaatctc cgccgataaa ggccagcccg ccggccagaa aaccgatcgt caccgcggcc
     4921 gcccccccgt aatgaaaggc ctcttccagg agtcccagag tgagggcgca gataagaatc
     4981 ccggaaccga aagccatcat ccgggcggtt gcccgctgcc ggagccggaa tcgcagggcg
     5041 gtgccggctc ccagcaccag ggccaggccg gaaaccgcgc cgtaaagaaa ggcggtcggg
     5101 aaggcgttcg gcgccatctt ttattccatt ttggccggta tttttatgcc taaacgacgg
     5161 gcggccttcg aaagggcggc ttggatttcg accgggttta tcaaaggaag cgccgggtta
     5221 tcaatcggcc ggatccggcc ggagtcgttc aggagatcca ccggaccgta acgggccggc
     5281 tgtttttttt cggataggat ggcggcataa gcgtcgccgc cgctgtagtg ccagaattcg
     5341 aatggatagg ccgcgaaacc gtgttcggcc atgatccggc tgatgccggc ccggttgcgc
     5401 cgggcggcgg ccggggcgta aggcgaagcc atcggggtca gttcgctcat ctccaggtag
     5461 ggggcgcccc ggtccacctc ctccccgttt ttgcgtttca agaccgagat gtccatggcg
     5521 ctgcccgaca tgtgggtgcc cagcttgggt atggtggtga ccaggacggt cagccgtcgg
     5581 aagacgaatt ccggggaagg aaaacgccct tccgtctccc agagcaggcg gcgcaggatg
     5641 gcatcgaaga gggccggcgg ccggttgagg ggattctgca tggaacgggt gcggtagccg
     5701 tcctcgatgc ggattctcca gccgtgccgg ttcatcgctt cggcggccga cagccagggt
     5761 ttgaccaggc cggcgcgcag gcggaagata ggccggtgtc ccggaatgcg ctgccggcga
     5821 aaaaaaacaa cctcgatgcc ggtgccgcgg agcgcctccg gtaatgaaac cagggcttca
     5881 cccgcatcgc aaaccgggtg agcgagcacc ttcatcatga attcataccc ggtttccatc
     5941 tccctggtcc agtaagccct ctttgccgcc tgactgcttc cgctgcctct catcaaggcc
     6001 tccgattccg gtgtatggtg gccgatcctg aaaagcggtc ctttccagtt aagtatacca
     6061 tcgcttaaat cgtcgtgcta taatttgagg atggcgggta taaaaaacca ggtggtggtg
     6121 gtcggcggcg ggccggccgg gatcatggcg gcgatccggg ccgccgagcg gggacggcgg
     6181 gtgctgcttc tggaacggac cggcgaactc ggcaagaaac tcctgatcag cggcggcggc
     6241 cgctgcaacc ttaccaaccg gcgcggcctg gacgagttcc tggcggctta ccataacggt
     6301 gcctttctcc gcaacgcctt tgcccgtttc ttcaaccagg aactgatgga cttcttcgag
     6361 tccggcggga tcaagctgac ggtggaacgg ggccagaggg tctttccggc cagccagtcc
     6421 tcggctgaaa tcgtcgcctt cctgaggcgc cggctggaag ccgcccgggt tcagctctgg
     6481 ttccgaaacc ggctgatccg ggcaaacccg ctcccgtccg gctgggaact ggttacccgg
     6541 caggatactt tttcgagtga aacccttctg ctggccaccg gaggccgctc ctacccggag
     6601 accggctcga ccggcgacgg ctacaatatc gcccggcggc tcggccacac catcaacaag
     6661 ccgcgggcgg ccctctgcgg gctggagttg aaagagaaat ggatccgcga ctggcagggt
     6721 gtcagcctgg aaaacgtggc gctcaccctt tcccagggcg accgggagct tggctcggaa
     6781 ttcggcgagg cgatctttac ccattacgga ctttccggcc cggcggtgct caatctctcc
     6841 cggattctta ccggtcgcga acaccaggcg gtcacccgcc tttcgattga tttcaagccg
     6901 gccctggacg ccggaaaact tgaccagcgg cttcaacggg agttgaacct gggccggcgc
     6961 caggtgaaga atatcctgcg gactctgctg ccgatgcgcc tggtggaaag gtttttgtcg
     7021 gtcgccgggc tgccggccga gaagccggcc caccaggtga ccgccgaaga gcgccgccgc
     7081 ttgctggagc aacttaaggc ctttcccctg accgtggcag ccttgaggcc ggccgacgag
     7141 gccatcgtga cccggggcgg agtggaggtg aaggagatcg acccgcgccg cctggaatca
     7201 cgccttcacc ccggcctctt cttcgccggc gaactaatcg atgtcgacgg cccgaccggg
     7261 ggattcaacc tgcaggccgc ctttacgacc ggctacgtgg ccggttccca cctttaagcg
     7321 gcggccgggt cacaaaaaac ggggcccgaa tttcggccgc gggagagttt tacccgccga
     7381 tttccgcctt cattccttcc gattcctggc tgcggaaatg gcgccactgg atacggtaaa
     7441 gccgggcata aaccccgccg gcggccagca gcgaatcgtg cgtacccgat tccacgatac
     7501 agccgccgtc gatcaccagt atcttatcgg cgtgcatgac cgtcgagagg cggtgggcga
     7561 tgacaaagga ggtccggtcc cgaagcaggt ggtcgatcgc ctgttgaata agagcctcgg
     7621 tctggttatc gaccgaggag gtcgcctcgt ccaggatgag gatgggcggg tttttgagca
     7681 gagcgcgcgc gatggccagg cgctgttttt ctcccaccga aagcttgatg ccacgttcgc
     7741 cgatctggtg cgcatacccg tccggcaggg cgttgatgaa ctggtcggca ttggccagct
     7801 tggccgcggc cacaatctcc tcgaaagtcg cctccaggcg gccgtaggcg atgttttcca
     7861 tgaccgttcc attgaagagg aacggctcct gcatgacgat cccgatgttc tcccgcaggt
     7921 cgtgcagctt cagttttttg acgtcgatcc catcgatgag aatcagcccc cgatcggcat
     7981 cgtagaaacg tggtatcaga ctgacgatgg tggtcttgcc ggccccggtg gctccgacca
     8041 gggccaccag ttcacccggc cgggcctcga agttgacggc atgcaggacc tcctggtccg
     8101 gctggtagga gaagctgacg tcgtggaaga ccacctcgcc ccgcaccggc ctgggcagcg
     8161 tgaccgcgtt ggacgcctcc aggatctggg gaaccgcgtc gatgatctcg aagatccgtt
     8221 cgctggcggc ccgggcgtgc tggagcatgt ggttgaacca gttcaactgg tggatcggct
     8281 ggtagaagag gcccaggtaa aagaggaagg agacgatttc gccggcgctc atgtctccgg
     8341 tcacaaccgc ctggcgcgct ccgaagagga ggaccaggaa ggttccaatc gaggtggtgg
     8401 taagaatcga aggggagaag aaggaccaga ggcggatggc ctggagattg gtggagaaat
     8461 agtcgctgct cttttcggcc agccggttta cctcgtaatc ctcccgcacg aaactcttga
     8521 tctcacgtat cccggagatg ctgtcctgga gcagggcgct gatctctccc atcttgcggc
     8581 ggacttcccg gtagatcagg tgggccttct gggttaccag ccaggccagg acaaggagga
     8641 tggggatggg gatcaggctg accagggcca ggcgccagtt gagccggaag agaatgacgg
     8701 tgatgcccag cagggtcatg acggcgatga tcaccgtgtc gatgccgtcc acgattaccc
     8761 gttccacggc ggagacgtcg ttggtgatcc gcgacatgat cgaaccggtc tggttgcggt
     8821 cgtagaaatt gaaggaaagg cgctgcaggt tgcggtagac atcctgccag agatcgtaga
     8881 tgacccgctg gccaagccgg ccgatcagga aacccttgag gccggaaaga acgtgggcgc
     8941 agatgtagac cgtaagaagg agcgtggcat tgcggtataa ttcggacagg gccttgcggg
     9001 cgaccgcctg gtcgatgatg gatccgatca gtcggggagg tgccaggtgc agcagcgtga
     9061 tcgtgaccga gatcaacagg gtggccgcta cctggagcag gtggggacgc aggtagacga
     9121 caagttttcc gatttccagc cgggcctcgg gattctcctc gttcggaagc cggttcattc
     9181 gatccatccg tccgaagtgc attccgccgc cgccgccccg cataccgccg ccaccgcctc
     9241 cccacatatc tatctccttt ctcgctgacc cccaagccat tttagcatta ttgacgcctg
     9301 agagccaaga tcgccaacgt tccggcggcg gtcggtcggg attgacacgg ttggcaaacc
     9361 gggactgctt gtattacaat caagaaagcg gccgccgcta ttctcaaaag gaggtgttat
     9421 gtaccgggtg cttctgatcg gggattcgat ccggatgggc tatgaacctt ttgtccgccg
     9481 ggaactggaa ggtctggccg aagtcctggg gccggagaag aacggggggg acagtgacaa
     9541 tgtcctcaga aatctgccgg aatggctcga gacgttcccg gccgacctgg tccatctgaa
     9601 ctgcggtttg cacgacatca agaggccttt tggttccagc agcaacggaa tcccccggtt
     9661 tcagtatgcc gacaacctgc gccagatctt ctcccacctg gtcgcccaca agataccggt
     9721 tatctgggcc accagcaccc cggttgacga aaaactgcac caccagaaca aaagcttcga
     9781 ccgcttcgag gccgatctgg agcattacaa cgcgaccgct ctggaggcct gccgggaatt
     9841 cggattggag gtggacgacc tgttcggtct tatcgaacgg gccggcaagt ccgcctatct
     9901 ccttgatgac ggggttcatt tcaagccgga gggttatcag ctgctcggcc gggaagtggc
     9961 cagggtcatc cgggccgccc ttgagaaatt gcgattgaaa tgaagaagag cgaacttccg
    10021 ggttatcgta aactggcgcg cgtctacctg gaaagggctg gcattatcat cactccggcc
    10081 gaggcccgtt cgatcgaagt ggccgatttc ggcctgggtg atttcgaaag gaccggcctg
    10141 ctcctgctgg tctacgtgag cactgatcgt tactgcgcca aggaactggt tctgctgccc
    10201 ggccaggtat gtccggagca ccgtcatccg ccccggagcg ggaaggatcc cggcaagcag
    10261 gagaccttcc gatgccggtg ggggagggtg gagctttttt tgccgggacg gccggcggcc
    10321 gttttttccg agccccggcc ggccgccttc acggtcggcc gccgggtgct ccttaaaccc
    10381 ggcctgcaat acacgatacc ggccaacacg cgccactggt tccgggccgg atccaagggt
    10441 gcggtggtct ccgagttctc atcggccaat tttgatgccg gcgatatttt taccgaccgc
    10501 cgggtgcggc ggctggcggg tgaaaaatga gcccgacccg ggtagcggaa cggcggcgga
    10561 aacgggccgg catctgctgc ggggggaact ggctgctcga tttcgtcaag attatcgacg
    10621 cctacccggc cgaggagcgg ctggccaaca tcctggagga gagcgtcgga aacggcggcg
    10681 ccccttacaa cgtcctggtt gacctggccc ggatcgatcc ggaccttccc ctggagggtg
    10741 tcggcgtcat cggccgcgac gagaattcaa gaagaatcat ggccgacatc cggcgttacc
    10801 ggatccggcc ggtcggaatc ttccgcaccg accgcctgcc caccgcctac accgacgtga
    10861 tgaccgaccg gaagaccggg cggcgcacct tctttcacaa ccggggggcc aaccgcctgc
    10921 tggggccgga acacttcgat ttcaagcgga tccgggccga gatcctgcat gtcggttacg
    10981 ccctgctcct ggacggactc gacgcctccg atccgcgtca cggcaccgga atggcccggg
    11041 tctttcactc ggcccgggcc gccgggctca agacctcgct ggacgtggta agcgaccagg
    11101 accgggcgcg tttccgacag gtggtcagcc cggccctgcc ggaggtcgat tacctgattc
    11161 tgaacgaatt cgaggccggg gcgctctccg gcctcgagct tgacccgaaa cggccgcgcg
    11221 agggcttcgc agcggccgcc cggcgcctct tcgacgccgg cgtcagggaa ctggtgatca
    11281 ttcacatggc agtgggagcc tggctcttca gccgtaaggg cgaagccttc tttcagccgg
    11341 cgcaccagtt gccggccgga ttcatccgga gcacggtcgg ggccggcgac gccttctgcg
    11401 ccggcatcct ttacggcctg gtcaagggcc tggatcaggc cggttgcatg aagtttgcca
    11461 atgccgccgc cgcgctcagc ctttcggcgg ccaccgcctc cgacggcctg ccccggcgcc
    11521 gccggatcga ggcgttcgcc cgccggacac cctgcctgaa tctctgaaag aaaaccaggc
    11581 ccgaagggat tttcgccgcc ggattctttt ggaagtcaat ccggcagttg tgatagaata
    11641 gaaacgcgaa ccagataagg gggccaaatg ttattcggaa agaaacaggt cgaggccggc
    11701 ggagtcggtt accggatcgt ggccgccgat gaccaggaag atgtccggac gatacttcag
    11761 aagggactga cggcggccgg ccacacggtc ttttcagcgg tggacggaga ggatgccctc
    11821 gaaaagatca agaacgagaa gccggacctg gttatcctcg atatccggat gcccaagctg
    11881 gatggtatcc acgcccttca ggtcatcaag aagagtccgc tcaccagaaa cctgccggtc
    11941 atcatgctca ccggcgagag ttccgacgag caactgctgg ccgggtacat gttcggggcc
    12001 gattactaca tcaccaagcc atttcggctt cagacggtcc tgaccgggat cacgatggtc
    12061 atgaaaccgg acaagcgtcc gggcaagtac accatctgaa ggcagcgagg attttgtttt
    12121 ccgggccgcg ccgaaaccgg cgcggctttt tttgtcccgg gaccgggccg ccgttgaacc
    12181 ggttcggcct tgctttactc gacggcgatc aaccggtaag ggattaccgg ttgaacccgg
    12241 ttcttgacct ggaccggttc ctgggcctcg gctctgattt tgccggccag ggcctggaaa
    12301 gtggcctcgc tgagcagaat gacgcccggc ccggcgatcg attccaaccg ctgggccacg
    12361 ttgacggcat ccccgatggc ggtataatcg aggcgccgcc ggctcccgat gttccccacc
    12421 accgcttcac ccgtgttgat gccgattccc acgccgaccc cgatcccaag ttccgtctgc
    12481 catcgggaag caagttcagc caggccggcc tgtatctcga gggcggtccg ggccgcggcc
    12541 agttccggtt cgtccaggtc cagcggcgct ccgaagaccg ccatggcgca atccccgatg
    12601 aacttgtcca gcgttccccg gttccggaag accgcctcgg tgaccagtcc gaggaattcg
    12661 ttgagaagtc tcaccacctc ttcgggcggc cgcccctccg aaaggcgggt gaaaccgcgc
    12721 aggtcggcaa agagcacggt tacccggcgc cggtcgccgc ccaggttaag cgaggcccgg
    12781 gccgtgagca gctgggagac gatctccggg gccaggtagc gggtaaaggt ctggcgcagg
    12841 aaggtctttt cccgccgctc ggcgaagtag ttgcggttgg tttcggcggc gaaggtaagg
    12901 aagaccgcca ggatcggcgc ggccaggtcg agccagagat ctgaacggaa gaggccccag
    12961 gcgaccgcca ggtatccggc caggatggcc gtcagcatca gggcgcgccg gcccagacgg
    13021 acctggtcgg tggccagccc ggccaggagg ccgagcagaa tcaggatcag caggcgggcc
    13081 ggagcgccgg gccggaccag gaaggagccg gacaggatat cctggagaac gccggcatgc
    13141 agcgcgaaca tcggaaagac cgggtcaacc ggcgtcggtc tcaggtcgcc gccgccgctg
    13201 accgccgagc cgatcagcac gattttgccc ttcagggtct caagcggaaa atcgggcttc
    13261 cggccggact gcgactgaat atgcgaggtc agcagggttt cccaggggca cccctggaaa
    13321 gtgcccaggc cgccgctgaa attgatccgc agtcggcctc tccggtcaac cggcagcatc
    13381 agcttgcgtc cggagcgatc ggtgatctcc aggcggtttc ccagaatttg aatcgtctcc
    13441 ggcttgatgt caaggtaggc gcaggccagg gccatgccca gggtgggata actcttgccg
    13501 ccggattcga cgaaaagcgg catgccccgg gtaaccccgt cggggtcggg cggagcgttt
    13561 atgatggcca ggttgccggg ttccttccgg aattgggcca gcggaaagac cggatcctca
    13621 ccggccggca accgaccggg tttctcttcg atcttccgaa agtaagcggc caggaagaga
    13681 ttcctggact ccgagatgga ctgggccagc atgcggtctt cgtcaggatt gga
//
LOCUS       DAOQYN010000021        13591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_5032_length_13591_cov_5.115765, whole genome shotgun sequence.
ACCESSION   DAOQYN010000021 DAOQYN010000000
VERSION     DAOQYN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5032_length_13591_cov_5.115765"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKN80_01740"
     rRNA            <1..490
                     /locus_tag="PKN80_01740"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            624..696
                     /locus_tag="PKN80_01745"
     tRNA            624..696
                     /locus_tag="PKN80_01745"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:657..659,aa:Ile,seq:gat)
     gene            complement(897..2027)
                     /locus_tag="PKN80_01750"
     CDS             complement(897..2027)
                     /locus_tag="PKN80_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34769.1"
                     /translation="MEKVYKVGIIGFGFMGRVRAYAYQNLKFFYQPAPLPVRLFGLCD
                     SNRALLDRAAADFGFELVTTDFRELIEHPEIDIINICSPNLFHREQILAAIAAGKHIY
                     CDKPLVCSAEEAAEIEAALAGYQGIGQMAFNYRFFPATLRARELVDQGFLGQPIAFRV
                     AYYHSGSVDPKKPMGWKQDRSFGGGVLYDMASHSIDMTCHLLGEYDRVLGSSLILYPS
                     RPDSSGRPVEVQVEDHVVVTARMKKGGLGVVEGSKVATGSQDELKFEIYGTGGALRWN
                     SIDPNFLDVFDQADPDVPLGGDLGFKRVAAVSRYPAPFSGFPGPKFTVGWLMGHVQSL
                     AAFLAAVRDRKPIEPSLAYGVYNIRVLEAVRRSEREERWVEV"
     gene            complement(2057..3046)
                     /locus_tag="PKN80_01755"
     CDS             complement(2057..3046)
                     /locus_tag="PKN80_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34770.1"
                     /translation="MKKTFLIDTGEIRRVLADELRARRLSSAPGDFTNRRSGNDVPLY
                     GSTGAANLHFELGLWPGETEERRAWGDRINSFQSEAGSFVSPSGREHATATALAALSL
                     LGTRPARPVGGLAPLDADRLPGWLAGLDWSGTHKDFWGGVTPVLASGLVDAGWREVLA
                     REIGGRIDPRRPLEVWCAEAEEPWRVISCIYHVTSGFDAAWIPYPAPELIRERLFGLG
                     YDRIRAGTKRTICTDFDYAVILDRLAHQRPEWFPEAHRLCRAVLADRVREWRENQDGL
                     LARASTHDLFCYTVGWAVYQRLLPESFTGPALYDTLHAPWLYRLPDRSRLADS"
     gene            complement(3074..3838)
                     /locus_tag="PKN80_01760"
     CDS             complement(3074..3838)
                     /locus_tag="PKN80_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34771.1"
                     /translation="MAGLVSILSADDWRLVVVVTFQATLLAYLPAPRAKALLLSLPIP
                     FTLATLALGRPVDATNALGLILLLAYTHAVRLLYCKVRLPILAAISLAAAGYAAAACL
                     VSPLVPAGPAAFWISLTAAWLLSLAIYLSMPPRDEPEHRTSLPVWLKMPAVAAVIFCL
                     AVIKYQLRGFLTVFPMVGLVAAYESRYSLWTLARQISLFLLASAPMLAVVRLAEPVAG
                     LEFALAAGWLVFLPILTCLRRFDAPGRMFSRGRPRT"
     gene            complement(3854..5458)
                     /locus_tag="PKN80_01765"
     CDS             complement(3854..5458)
                     /locus_tag="PKN80_01765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ34772.1"
                     /translation="MMEKSLFEVKPVDRDFYQERLEAFLPARMIDIHTHVWRRGFEEA
                     VAESRRRVVSWPSRVARDNPVEDLLETYRLLLPGKEVTPLIFATLNRLPDLEAANEYV
                     SRSAGGHALPSLIFAQPAWSGSELDERIRAGGFIGAKVYLSFAPDYLPADEIRIFDFI
                     PPHQLEALDRRGRLLMLHLPRPGRLKDPVNLAQLLELEARYPRLRVVVAHVGRAYCPE
                     DVGPAFEVLASTRRLLFDISANTNAWVFERLLRAVGPERVLFGSDLPITRMRMRRICE
                     GGRYINLVPAGLYGDVSGDPNLREVSGPEAERLTFFLYEELDAFRQAARAVGLDSGGI
                     EAVCYRNARNLLDEVSATPRPQLQMVWRGDRPERPRRPDRYRLRSYRPGDEAGYVELM
                     RSAGFQDWDRAAEVLRRAIPEGLLFLVERRTGRLVGTAACLHAPLPGQPGRGELGWVA
                     VDPGHTGRGLGRLVCAAALRRFLKSGYRNLQLYTDDFRLPAVKIYLGLGFVPLLDGPG
                     LEERWRAVCGQLGLDPDRVLAAAGRA"
     gene            complement(5489..6754)
                     /locus_tag="PKN80_01770"
     CDS             complement(5489..6754)
                     /locus_tag="PKN80_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34773.1"
                     /translation="MAVRKSGARNIRVGVVGVGRGSSFARTCGWVGLELAALCDTWEA
                     KLKSVAGQYGVAAYTDFDRMLEKEDLDAVILANYFHQHAPLAIKALAAGKHVMSETMA
                     CKTPAEGVALARAVERSGRIYLFAENYPYFAYNQEMRRLYRAGEIGVMQYGEGEYNHP
                     VDSRGMNRLAPGLNHWRNWLPSTYYCSHGLAPVMFITDTRPIRVNALSVHRFAEDREK
                     LHVRRGDPGSVILCRMDNGSVVRIMGLMMRGHSIWYRIHGTRGLMENLRPAGSENLLR
                     VKHEAWDLEPGGVRERIYLPEFPDYARAAARTGHGGGDFFMDYHFAEAIRTGRAPYFD
                     VYRGLDMALVGIQAWRSALADGTPFEVPDFRKEAARRRYAEDHFSPWPEDAGPGQPPP
                     SIKGFPKPDPKAAAFARKVWREDCGYTGR"
     gene            complement(6781..7914)
                     /locus_tag="PKN80_01775"
     CDS             complement(6781..7914)
                     /locus_tag="PKN80_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34774.1"
                     /translation="MAETAAGNSQLVLDTIAHRSVKGIPIGLLHIMEHGMIERLAGAQ
                     PGDYRREPRRVYLRMQKNCGVGFIDQFIPENPLEMGAHGYEGRERSATTGAERIERDG
                     LLIDSPEAVVDHLERIVFPWLAKAAAGFDEAKRCREILASEAAVQAELGPEILKTGFG
                     FIRFPCFAYSTYGYANYFMAYALYPEVMERHFSLQADYCRLNNRAAARAYREGNLPPL
                     FRLDYDMADSRGTLVNIKSLDRIWFPHFARSLEPVLKSPVRLIWHCDGNLMEMVPRLL
                     EAGLAGFQGFQYEDGMDYERIGRMKTRSGEPLFIWAGVSVTRTLPRGGPEDVKRELDW
                     LVRTGPPTGLVLGVTSSVAPGVSYDNLRTLTEGLRYYREHGRG"
     gene            complement(7915..9009)
                     /locus_tag="PKN80_01780"
     CDS             complement(7915..9009)
                     /locus_tag="PKN80_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34775.1"
                     /translation="MELEYKNDWPETAERYRAWWAGEYFGRCGLWVTGLREGVPEEAP
                     PAPPARSGARFLSFDYLAAANEHAHRRTFYGAEAFPGWHPGYPGWNCAAAFLGCPVRL
                     DDHTGWLDPIVPAGPLTDFDFRKLTLDEGNRWWQLHLEMLRFEARQAAGRSIPSLGAF
                     GASGDTLAALRGSSPLLYDLVDCPEYVAAFETHLMDVWIEIYRRSQAVLAPAAEGTTC
                     WFTLWAPGKFYSIQNDFAYMISPAMFRKVFLPEIERQTRFLDHALYHLDGVGNFNHLE
                     ALLELPRLRAVQVLPGAGKPGPLHYLEVLRRVQSAGLNLHISIPAAEVPEALERLSAR
                     GLFIATSCRSEAEARHLIRECEERSVPRRF"
     gene            complement(9009..10118)
                     /locus_tag="PKN80_01785"
     CDS             complement(9009..10118)
                     /locus_tag="PKN80_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34776.1"
                     /translation="MDKIRLGIVGAAGRGRSFKQACLASGLVEIRAVCDLNEVRLEEA
                     RQNLGAAEKYLDYREMLEKSDLQAVILGTPMPCHVPQAVAALERDIHVLCEVPAGVSI
                     DECRQLTAACRNSKGVYMMAENYTYRKQNVLVRELVRRGLFGTAYYGEGEYLHELKEL
                     NEITPWRRRWQTGVNGVTYGTHSLGPLLQWLEGDRVTSVCCAGSGRHYRDPRGHLYEN
                     EDSCLMLCRTVRGALLKIRVDMLSDRPHAMTNYALQGTDGAYESSRAPGEPDRIWLRS
                     RAGSPKAWLNLADLEAEFLPEYWKAGQAEAARSGHGGGDYFEVMDFLDSIRGRRPPPV
                     GIHEAMDMTLPGLVSQESINREGAWLPVPDSRGWP"
     regulatory      10231..10428
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10560..12383
                     /locus_tag="PKN80_01790"
                     /pseudo
     CDS             10560..12383
                     /locus_tag="PKN80_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            12397..13155
                     /locus_tag="PKN80_01795"
     CDS             12397..13155
                     /locus_tag="PKN80_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNQ34777.1"
                     /translation="MRPVGGLCLSISGHLLLALLLLAMPLLQPYERYSEPVIFVNLDR
                     LPPVAERTSRPPQEPPGPEPIRPAPIPAPSRIIPEASRLPSPRPASPAVEPIETPRST
                     TLITPWPEPFIPPLPKGPAGPESHVPAPPAAAERETIAREPVRSANEDRVDLDRPAES
                     DYARLVRRRIMENLAYPAAARRRGQAGAVRLRFELDSEGRLVRSSVESGSGIPALDRA
                     AVESIRKSAPFPAWTGSEPLSNRVFFITLRFHLN"
     gene            13321..>13591
                     /locus_tag="PKN80_01800"
     CDS             13321..>13591
                     /locus_tag="PKN80_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34778.1"
                     /translation="MEKKNKETLPWWKQNGIIMAGGWHPITGRLRAGNQPDNVEEEYD
                     WEYTEAHILRLKELGIKLLVSQFDRGLGDTDQAEDQERARLQAELC"
BASE COUNT         2526 a         4464 c         4427 g         2174 t
ORIGIN      
        1 cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct tgcccttagt
       61 taccggaacg taagtttcgg accctaaggg gactgccgca gatgatgcgg aggaaggtgg
      121 ggacgacgtc aagtcagcat gccccttatg tcctgggcga cacacgtgct acaatggccg
      181 gtataaagag acgcaaagcc gtgaggtaga gcaaatccca aaaaaccggt cccagttcgg
      241 atcggggtct gaaactcgac cccgtgaagc cggaatcgct agtaatcgcg gatcagccat
      301 gccgcggtga atacgttctc gggccttgta cacaccgccc gtcacgtcaa ctgagtcggt
      361 tatacccgaa gtcgtctgcc taaccggcgg ggcaacctgt tggagggcaa cgcctaaggt
      421 gtgtccggca aggaggacga agtcgtaaca aggcagccgt acgggaacgt gcggctggat
      481 cacctccttt caaggagtag taactcctaa aaaagtaaaa ccgaggtgat aactcggtgt
      541 gtaaaaacaa acaaaaaagc tgacatacac ccatatcctt tttgacaaag aagcgacctg
      601 ggcaacctcg tcgctttttt gttgggctca tagctcaggt ggcagagcac tgtgctgata
      661 acgcaggggt cgaaggttca agtccttctg agcccaccgg taaagaggca ttgagagggg
      721 cggcttgaat aaaggccgcc cctttttttg tgcggaacgg gaaggaggaa tcggggaatc
      781 ggcagcgtgt cttgaaggag aaattaatcc cccatcggcc ggtcagttga agccggccga
      841 cgaagcggcc gggcccggcc acccgggaga aaaaacgggc ccaaaacctt gtaactctaa
      901 acctcgaccc agcgttcctc tcgctcggaa cgacgcaccg cctccaggac ccggatgttg
      961 taaaccccgt aagccagcga cggttcaatc ggcttccggt cacggacggc cgccagaaag
     1021 gcggccaggg actggacgtg acccatcagc cagccgacgg tgaacttggg gcccgggaag
     1081 cccgaaaagg gagccgggta acggctgacc gcggccaccc gcttgaagcc gagatcaccg
     1141 cccagcggta cgtccgggtc ggcctggtca aagacgtcca ggaagttcgg gtcaatcgag
     1201 ttccagcgca gcgccccgcc ggtaccgtaa atttcaaatt tcaactcgtc ctggctgccg
     1261 gtggccacct tggaaccctc gaccacgccg aggccccctt tcttcatccg ggcggtcacc
     1321 acgacgtggt cctcgacctg gacttccacc ggccggccgc tcgaatcggg ccggctcgga
     1381 taaagaatca agctgctgcc caagacccgg tcgtactccc cgagcaggtg gcaggtcatg
     1441 tcgatcgaat ggctggccat gtcgtaaagc accccgcccc cgaaggatcg gtcctgtttc
     1501 cagcccatcg gtttcttggg atccacgctg ccgctgtggt agtaggcaac ccggaaggca
     1561 atcggctgac ccaggaatcc ctggtcaacc aattcccggg cccggagcgt ggccgggaag
     1621 aaccggtagt tgaaggccat ctggccgata ccctggtagc cggccagggc cgcttcgatt
     1681 tcggcggcct cttcggcact gcaaaccagc ggtttgtcac agtaaatatg cttgccggcg
     1741 gcgatggcgg ccaaaatctg ctcgcggtga aaaaggttgg gagagcagat gttgatgatg
     1801 tcgatctccg ggtgctcgat cagttcccgg aagtcggtgg tcaccagctc aaagccaaaa
     1861 tcggcggccg cccggtccag caaggcccgg ttgctgtcac agaggccgaa gaggcgtacc
     1921 ggcagcggcg ccggctggta aaaaaactta aggttttgat aggcgtacgc gcgcacccgg
     1981 cccatgaagc caaaaccgat tatccccacc ttgtaaacct tttccatgct tcttctcctt
     2041 gttccgatcc gggtcctcag gaatcggcca gccgggaacg atccggcagc cggtagagcc
     2101 agggggcgtg caaagtatcg tagagggccg gcccggtgaa agattccggc agcagccgct
     2161 ggtacaccgc ccagccgacc gtgtagcaga agagatcgtg cgtgctggcc cgggccagca
     2221 gtccatcctg attctcccgc cattcccgga cccggtccgc caggaccgcc cggcagagac
     2281 ggtgcgcctc cggaaaccac tccggccgct ggtgggcaag ccggtccagg atgaccgcgt
     2341 aatcgaaatc ggtgcagatg gtccttttcg tacccgcccg gatccggtcg tagccgagtc
     2401 cgaacaggcg ttccctgata agttcggggg ccggataggg gatccaggcg gcgtcaaagc
     2461 cgctggtaac atggtaaatg cagcttatca cccgccaggg ctcttcggct tcggcgcacc
     2521 agacctcgag gggccgccgc gggtcaatcc ggccgccgat ctcccgggcc aggacctccc
     2581 gccatccggc gtcaacgagt ccggaagcca gcaccggagt caccccgccc cagaagtcct
     2641 tgtgggtgcc gctccaatcc aggccggcca gccagcccgg gagacgatcg gcgtcaagcg
     2701 gggccagacc gccgaccgga cgggcgggcc gggttcccag gagggagagg gcggccaggg
     2761 ccgtggccgt ggcgtgctcc cggccggaag gcgagacaaa acttccggcc tcggactgga
     2821 aggaattgat ccggtctccc caggcccgcc gttcctccgt ttcgcccggc cagagtccga
     2881 gctcgaaatg gaggttggcg gcgccggtcg atccgtagag cggcacgtcg ttgccggaac
     2941 ggcggttggt gaaatcgccc ggcgccgaag ataggcggcg ggcccgaagt tcgtcggcga
     3001 gtacccgcct gatctccccg gtatcaatca aaaacgtttt tttcatcttc tctccggcgt
     3061 ccgccaggcg gtatcaggtc cgggggcgtc cccgggaaaa catccggccg ggtgcgtcaa
     3121 aacggcgcag gcaggtcaaa atcgggagaa agaccagcca gccggcggcc agggcgaatt
     3181 ccaaacccgc gaccggctcg gccaaccgga ccacggccag catcggggcc gaggcaagca
     3241 ggaagagtga aatctgccgc gccagggtcc agaggctgta acggctttcg taggcggcca
     3301 ccaggccgac catcgggaag acggtcagga aaccccgcaa ctgatatttt atcaccgcca
     3361 ggcagaagat cacagccgcc accgccggca tcttcagcca gaccggcagg ctggtccggt
     3421 gctccggctc atcccggggc ggcatcgaga ggtagatggc cagggacagc aaccaggcgg
     3481 ccgtcaggga tatccagaag gcggccggac cggccgggac caggggggag accaggcagg
     3541 cggcggccgc atacccggcc gcggccaggc tgatggcagc caggatgggc agccggacct
     3601 tgcaatagag cagccggacc gcgtgcgtat aggccagcaa aagaatcagg cccagggcgt
     3661 tggtggcgtc caccggccgg ccgagcgcca gggtggccag ggtgaaggga atcggcagcg
     3721 aaagaagcag ggccttcgcc ctcggcgccg gcaggtaggc cagcagggtc gcctggaaag
     3781 tcaccaccac gaccagccgc cagtcgtcgg cggataagat cgaaaccagg ccggccatct
     3841 tttccaccgc ggttcaggcg cgcccggccg cggccaggac ccggtccgga tcaaggccaa
     3901 gctggccgca gaccgcccgc cagcgttcct cgagtccggg cccgtccaga agcggtacaa
     3961 agccaagccc caggtaaatc ttgaccgccg gcagacggaa atcgtcggtg taaagctgga
     4021 ggttccggta gccggacttc aggaagcgac gcagggccgc cgcgcagacc aggcggccca
     4081 acccccggcc ggtatggccc ggatcaaccg ccacccagcc gagttcaccc cggcccggct
     4141 gcccgggaag cggcgcgtgc aggcaggcgg ccgtgccgac caggcggccg gtccgtctct
     4201 caaccaggaa aagcagtccc tccggaatgg cccgccggag aacctcggcc gcccggtccc
     4261 agtcctgaaa accggcagag cgcatcagtt ccacgtaacc ggcctcatcg cccggccggt
     4321 agctgcggag ccggtaacga tccggccgcc gcggccgctc cggccggtcg ccgcgccaga
     4381 ccatctgcaa ctggggccgg ggcgtggcgg agacctcatc cagcaggttg cgggcgttgc
     4441 ggtagcagac cgcctcgatc ccgccggaat caagaccgac cgcccgcgcc gcctggcgaa
     4501 aggcgtccag ttcctcgtat aggaagaagg tcagccgttc ggcctccggt ccggagacct
     4561 cgcgcaggtt agggtcgccg gagacgtcgc cgtagaggcc ggccgggacc aggttgatgt
     4621 aacggccgcc ttcgcagatg cggcgcatgc gcatccgggt gatcggcagg tcgctgccga
     4681 agaggacccg ttccgggccg accgcccgca gcagccgttc gaagacccag gcattggtgt
     4741 tggctgagat gtcaaagagg aggcgccggg tcgaggcgag cacttcgaag gccgggccga
     4801 catcctcggg acaataggcc cggccgacgt gggcgacgac tacccgcagg cgcgggtagc
     4861 gcgcctccag ctccaggagc tgggccaggt tgaccggatc cttcaagcgg cccggccggg
     4921 gcaggtggag catcaaaaga cggccgcgcc ggtcgagcgc ctcgagctgg tggggcggaa
     4981 tgaaatcgaa gatgcggatc tcgtccgccg gcaggtaatc gggcgcgaag ctcaggtaaa
     5041 ccttggcccc gatgaaaccg ccggcccgga tccgctcgtc aagttccgaa ccgctccagg
     5101 ccggctgggc gaagatcagg gacggcagcg cgtggcctcc ggcggaccgg ctcacgtatt
     5161 cgttggccgc ctccaggtcc gggagccggt tgagcgtggc gaagatcagc ggcgtaacct
     5221 ccttgcccgg cagcaggagc cggtaggtct ccagcaggtc ttcaaccggg ttgtcgcggg
     5281 caacccggga cggccagctg accactcggc gacggctctc ggccacggcc tcttcaaacc
     5341 cgcgccgcca gacgtgggtg tgaatgtcaa tcatccgggc gggcaggaag gcctccaggc
     5401 gctcctggta aaagtcccgg tcaaccggtt tcacttcgaa taaacttttt tccatcatgc
     5461 cctggctcct tggccggtgt cgccgccgtc agcggccggt gtaaccgcaa tcctctcgcc
     5521 agaccttgcg ggcgaaggcg gccgctttcg ggtccggctt cggaaaaccc ttgatgctcg
     5581 ggggcggctg gccggggccg gcatcctcgg gccagggtga aaaatggtct tccgcgtagc
     5641 gccgtcgggc cgcttccttc cgaaagtccg gaacttcgaa gggcgtgccg tcggccaggg
     5701 ccgaccgcca ggcctggatg ccgaccagag ccatgtccag gccccggtaa acatcgaagt
     5761 aaggcgcccg tcccgtccgg atggcctcgg cgaagtgata gtccatgaag aaatcaccgc
     5821 cgccgtggcc ggtccgggcc gccgcccggg cgtaatcggg aaactcgggc aggtagatcc
     5881 gttcccggac tccgcccggc tccaggtccc aggcttcgtg cttgacgcgc agcaggtttt
     5941 cgctgccggc cgggcgcagg ttctccatca ggccccgggt gccgtggatg cggtaccaga
     6001 tgctgtggcc gcgcatcatc aatcccatga tccggactac cgaaccgttg tccatccggc
     6061 agaggatcac cgagcccggg tcgccgcggc gcacgtgcag cttctcgcgg tcctcggcga
     6121 agcggtgaac ggagagggcg ttgacccgga tcggccgggt gtcggtgatg aacatgaccg
     6181 gcgccaggcc gtgggagcag tagtaggtgg agggaagcca gttgcgccag tggttcaggc
     6241 ccggcgccag ccggttcatg ccccggctgt cgaccgggtg attatactct ccctccccgt
     6301 actgcatgac gccgatctcg ccggcccggt agaggcggcg catctcctgg ttgtaggcga
     6361 agtaggggta attttcggcg aacaggtaga tgcggccgct tcgctccacc gcccgggcga
     6421 gagccacgcc ctcggccggg gtcttgcagg ccatcgtctc gctcatcacg tgcttgccgg
     6481 ccgccagagc cttgatggcc agcggcgcgt gctggtggaa gtagttggcc aggataaccg
     6541 catccaggtc ctctttttcc agcatccggt caaaatcggt gtaggcggcc acgccgtact
     6601 ggccggccac cgatttcagc ttcgcctccc aggtgtcgca gagggcggcc agttccagac
     6661 caacccagcc gcaggtccgg gcgaaagaac tcccccggcc gacgccgacc accccgaccc
     6721 ggatgtttct tgccccgctt ttccgaaccg ccatttttca aatcctcccg aaggccagtt
     6781 tcatccccgg ccgtgttccc ggtagtaacg caatccctcg gtcagggtgc gcaggttatc
     6841 ataggagacc ccgggcgcga ccgaactggt tacaccaagg accagcccgg tcggcggtcc
     6901 ggtgcgcacc agccagtcca gctccctttt cacgtcttcg gggccgcccc ggggcagggt
     6961 ccgggtgacc gaaaccccgg cccagatgaa gaggggttcc ccgctccggg tcttcatccg
     7021 gccgatgcgc tcgtaatcca tgccgtcctc gtactgaaac ccctggaaac cggccaggcc
     7081 cgcctccagc aggcgcggca ccatctccat caggttcccg tcgcagtgcc agatcagccg
     7141 gaccgggctc ttcaagaccg gctccaggct gcgggcgaag tgcggaaacc agatccggtc
     7201 gaggctcttg atgttgacca gggtcccgcg ggaatcggcc atgtcgtaat cgaggcggaa
     7261 gaggggcggc agattgccct cacggtaggc ccgggcggcc gcccggttgt tgagccggca
     7321 ataatcggcc tggagtgaga aatggcgttc catcacttcc gggtagaggg cgtaggccat
     7381 gaagtagttg gcgtatccgt aggtgctgta ggcgaaacag ggaaagcgga tgaagccgaa
     7441 cccggtcttc aggatctccg gacccagctc ggcctggacg gccgcctcgg acgccaggat
     7501 ctcccggcat cgcttcgctt cgtcgaaccc ggccgccgcc ttcgccagcc aggggaagac
     7561 gatccgctcc aggtggtcga ccaccgcctc gggcgaatcg atgaggagac cgtcccgctc
     7621 gatccgttcg gcgccggtgg tggcgctccg ttcgcgtcct tcgtagccgt gggcccccat
     7681 ttccaggggg ttctcgggaa taaactggtc gatgaaaccc accccgcagt tcttctgcat
     7741 ccgcaggtag acccggcgcg gctcccggcg gtaatcgccc ggttgggcgc cggccagccg
     7801 ttcgatcatc ccgtgctcca tgatgtgcag caggccgatc gggatgccct tgacggaacg
     7861 gtgcgcgatg gtatcgagta ccagctgtga atttccggcg gcggtctctg ccatttaaaa
     7921 cctcctggga acggaacgtt cctcgcattc cctgatcaga tggcgggcct cggcttcgct
     7981 ccggcagctg gtggctatga agaggccgcg ggccgaaagg cgctccaggg cctcgggcac
     8041 ctcggcggcc gggatggaga tatgaaggtt gagtccggcc gactggaccc gccgcaggac
     8101 ttcgaggtag tggagcggcc cgggcttgcc ggccccgggc agcacctgga cggcccgcag
     8161 gcgcggcagt tcaaggaggg cctccaggtg attgaagttg cccacgccgt ccaggtggta
     8221 gagggcgtga tcgagaaaac gcgtctgtcg ctcgatctcc ggcagaaaaa ccttccggaa
     8281 catggccggg gagatcatgt aggcgaaatc gttctggatg gagtagaact ttccgggcgc
     8341 ccagagcgtg aaccagcagg tggtcccctc ggccgccggg gcgaggaccg cctgactccg
     8401 tcggtaaatc tcgatccaga cgtccatcag gtgggtttca aaggcggcca cgtactccgg
     8461 gcagtcgacc aggtcgtaga gaagcggcga gctgccccgc agggcggcca gcgtatcgcc
     8521 ggaggcgccg aaggcgccga ggctcggaat ggagcgcccg gccgcctgcc gggcctcgaa
     8581 gcggagcatt tccaggtgca gctgccacca gcggtttccc tcgtccaggg taagcttccg
     8641 gaagtcgaaa tcggtcaggg ggccggccgg aacgatcggg tcgagccagc cggtatgatc
     8701 gtccaggcgg accgggcagc ccaggaaggc ggcggcgcag ttccagcccg gatagcccgg
     8761 gtgccagccg ggaaaggcct cggccccgta aaaggtccgc cggtgcgcgt gttcgttcgc
     8821 ggccgccagg taatcgaagg agagaaaccg ggccccgctc cgcgccggcg gggccggcgg
     8881 tgcttcttcc ggaacgcctt cccgcagtcc ggtcacccag aggccgcagc ggccgaagta
     8941 ttcgcccgcc caccaggccc ggtagcgttc ggcggtttcc ggccagtcgt tcttatactc
     9001 gagttccatc acggccagcc gcgcgaatcg ggaaccggca gccaggctcc ctcccggttg
     9061 atcgactcct ggctgaccag gcccggcagg gtcatgtcca tcgcctcgtg gatgccgacc
     9121 ggcggcggcc ggcggccccg gatgctgtcc aggaaatcca tcacctcgaa gtaatcgccg
     9181 ccgccgtggc cggaacgggc ggcctcggcc tggcctgcct tccagtattc gggcaggaac
     9241 tccgcctcca ggtcggccag gttgagccag gccttggggc tgccggcccg ggagcggagc
     9301 cagatccggt ccggctcgcc cggcgcccgg gacgattcgt aggcgccgtc ggtgccctgc
     9361 agggcatagt tggtcatggc gtggggccgg tcggagagca tgtccacccg gattttgagc
     9421 agggcgcccc gcaccgtccg gcagagcatc aggcagctat cctcgttttc gtagaggtga
     9481 ccgcgcgggt cccggtaatg gcggccgctt ccggcgcagc agaccgaggt cacccggtcg
     9541 ccctccagcc actggagcag cggtcccaga ctgtgggtgc cgtaggtgac gccgttgacg
     9601 ccggtctgcc agcgccggcg ccagggggtg atttcgttga gctccttcag ctcgtgcagg
     9661 tattcgccct cgccgtagta agcggtgccg aagaggccgc ggcggaccag ttcgcggacg
     9721 agcacgttct gtttccggta ggtgtaattc tcggccatca tgtagacgcc cttcgaattg
     9781 cggcaggcgg ccgtcagctg gcggcattca tcgatcgaga ccccggccgg cacctcgcag
     9841 agtacgtgga tgtcacgttc gagggcggcc acggcctggg ggacatgaca gggcatcggg
     9901 gtcccgagga ttaccgcctg gaggtccgat ttttccagca tctcccggta atcaaggtac
     9961 ttctcggccg cgccgaggtt ctgacgcgcc tcctccagcc ggacctcgtt gagatcgcag
    10021 accgcccgga tctcgaccag gccgctggcc aggcaggcct gcttgaaaga gcggccgcgg
    10081 ccggcggcgc ccacgatacc cagccggatt ttatccatga tcgccccctt ccagccctgg
    10141 cgataagaga ccgaacaaga aaatatccct attgtatata aaacccgtcc gaaaagcaaa
    10201 aaaccgaaga attgacgcgc tccggaggcc ggatgtataa tttaccccgg ttccttgaga
    10261 atcaaagggc gaacttaacg gaagacggtg cgaatccgtc gcgggcccgc cgctgtaacc
    10321 ggtatcagcc gctcgaatgc cactgtcccc tcgccggggg gcgggaaggc gcggccgcag
    10381 gaagccggga gccagaagac gaagttcgcc gcgatcagaa acgccctcgc ggaacggggc
    10441 gggaccgggt gaaaaacggc atcccctgtc ggtcgcaatg accggccggg gctttttttt
    10501 aatcccggga tccctctaac cgcaaaatcc ggagaaaaaa gatgggaaga atcattaaaa
    10561 tgttaatcct ggccggcctg gccgccggaa cggccatccc ccccgctttt ggggccgaac
    10621 cggccggcgc cgagctcgag gagatcgtca tcaccgccac ccggacgccg gaaccggccg
    10681 ataagctccc ggtggttgtc aattacctgg accgcccggt catcgagact tccggggccg
    10741 ccaatctggg agagctgctg ggtggaaccg ccggggtgac cgtcaagagc cagggtggac
    10801 cgggcagcca ggtctccctt ggcctgcgcg gttcgaccag cgaacaggcg ctcctgctga
    10861 tagacggccg tccccttaac ctgccggcct ccggcctctt tgacctcggc ctctttccaa
    10921 tggaactggt cgaaagggtc gaggtcctgc gcggtccagc ttcggccatt tacggggcca
    10981 acgccctgag cggggtggtc aacctgatca cccggcgcat cccggccgac cctgaattcg
    11041 caaccaacct gagcttcggc accttcgaca cctggcgggc cggggcctcc ttcggccgga
    11101 ccgtcggccg cttcggctac ctggtgggcg gatcgggcct ccagtccgga ggcgaccggg
    11161 agaacagcgc cgcccgggaa gaacacctct tcggcaaggc ctccttaaac ctgggcgata
    11221 atgccgagct gaccctgtcg ctcggcagcg atttccaggt aagcgaggtg cccggtccga
    11281 ccgcctttcc ctccccgctg gcggagcagc ggaccgagga ggagtggcag gagctcggct
    11341 accgtttcta ccgggacgcc ggggaattcc aggcccgcct ctaccggcgg cggaaccgac
    11401 tccactaccg caaccccgac ccgggctggc cggttgacac catcacccgc aaccgccagg
    11461 atggactgga gatccagaac agctcactgg tcgcagaacg ccaccagctg accttcggcg
    11521 ccggatacct ggccgaccgg gtggacgggg acgccaccgg ccaacacgaa ccggacagcc
    11581 ggaatctcta cctgcaggac cagattgacc tgggccggct taacctcctg ctgggcggcc
    11641 gctatgacga ccactcggtc ttcggttcgg agttcagccc gaggctggcc ggccgggtcg
    11701 ccctgaacga acgaactgcc ctgcgcctgg ccttcggccg atcctaccgg gccccgacag
    11761 tcaacgaact ttactggttc gaggaatggt ggccgggcgc cggctccttc ggaaatgccg
    11821 gcctgcggcc cgaaaagggg acctcttacg aagcgggact ggaccataat ttcaatcggc
    11881 gggtctccct gcggaccacc tatttccaga acgaggtcaa ggacctgatc agctgggtcg
    11941 aagtagtgcc ctggaccggt tacgaaacga tgaatatcgg ccgggcccgg atttccgggc
    12001 tggagacgga actgcgctgg gaggccagtc ccggactccg cttcaccgcc agccacaccc
    12061 gcctcgatac ccgtgaccgg cagaccggca aagaactgcc gtaccgaccg ggccaggagt
    12121 tcgggctgat cggggagatc gccgtccgcg gctggaacct ttacctctag gccgggtacc
    12181 gcgatccggc ctgggccgac ccggccaata cccggaaagt ggaggatcac ctgctgcttg
    12241 gagcccggct cgcccgccag gtccggcccg gcctggagtt cttcctgcag ggcgaaaacc
    12301 tcctgaacga gaattacgaa atcctgagcg gttacccgat gcccggtgcc agcttcggca
    12361 tcgggataaa cgcccgtttc tgacccggga ggtccgatgc gtcccgtcgg cggcctctgc
    12421 ctttcgattt ccggccacct tctgctggcg ctcctgctcc tggccatgcc gctgcttcaa
    12481 ccgtatgaac ggtactccga accggtcatc tttgtaaatc tcgaccggtt gccgccggtc
    12541 gccgagagaa ccagccggcc cccgcaagag cctcccgggc cggaacctat ccggccggca
    12601 ccgatcccgg caccatccag gatcatcccg gaagcatccc ggctgccgtc cccccgtccg
    12661 gcttccccgg ccgtcgagcc gatcgaaaca ccccggtcga caacgctgat cactccctgg
    12721 cccgaaccgt tcattccgcc cctgccgaag ggcccggccg gaccggaaag tcatgtcccg
    12781 gcgccgccgg ccgcggccga acgggagacg atcgccaggg aacccgtccg gtctgcgaat
    12841 gaagaccggg ttgacctgga ccggccggcc gaatccgatt acgcccggct ggtgcgccgc
    12901 cggatcatgg agaaccttgc gtatccggcc gcggcccgtc ggcggggcca ggcgggggcc
    12961 gtccgactcc ggttcgaact ggactccgaa gggcggctgg tccggagttc agttgaatcg
    13021 ggatccggga tcccggccct ggaccgggcc gcggtcgagt ccatccggaa atcggcccct
    13081 ttccccgcct ggaccggaag tgaaccactg tcgaaccggg tcttttttat cactctccgc
    13141 ttccacttga actgaagtca ggattgaaaa ttgtctaatt ataccggttg acttttcccc
    13201 gttgttactc tatcataaat ggaatacgcg cctttaaaga tggcgcgacc gggaagaccc
    13261 cggtcctcca accaggaacc agtaccagca attactttca atcaacaagg agccggcacg
    13321 gtggaaaaaa agaataagga aacccttccc tggtggaagc agaacggcat catcatggcc
    13381 ggcggctggc acccgatcac cggccgcctt cgcgccggta accaacccga caacgtggaa
    13441 gaggagtatg actgggagta taccgaggcg cacatcctcc ggctgaagga gctgggtatc
    13501 aaactgctgg tcagccagtt cgaccgcggc ctgggcgaca ccgaccaggc cgaggatcag
    13561 gaacgggccc gcctccaggc cgaactctgc c
//
LOCUS       DAOQYN010000022        13581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_5034_length_13581_cov_4.905367, whole genome shotgun sequence.
ACCESSION   DAOQYN010000022 DAOQYN010000000
VERSION     DAOQYN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13581
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5034_length_13581_cov_4.905367"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..444
                     /locus_tag="PKN80_01805"
     CDS             40..444
                     /locus_tag="PKN80_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNQ34779.1"
                     /translation="MNRSRVREEILDVFLRAERHLTADQLYQLARRRLPSVGYATVYR
                     SLKIFCDCGLCRPIKLENEPVRYEHLYRHDHHDHLVCTRCGRLVEVFDPTLEQLQEKL
                     GRKHGFQMERHRLELYGICPECRKKGGPDDRN"
     gene            431..991
                     /locus_tag="PKN80_01810"
     CDS             431..991
                     /locus_tag="PKN80_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNQ34780.1"
                     /translation="MTEINNVRKNGKRPFEAGPGRPRLALVGSPNVGKSSLFNRLTNS
                     YLIVSNYPGTTVEVSRGQARIGEVEFEVIDTPGLYSLLPLTEEERVARDILRLERPEL
                     VLHVIDAKNLERMLPLTLQLREAGLSLVLVLNIMDDAEKLGLRIDLERLERELGLPAA
                     ATVSTTGRGIDKLKEIILTAHEQRKT"
     gene            972..2429
                     /locus_tag="PKN80_01815"
     CDS             972..2429
                     /locus_tag="PKN80_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNQ34781.1"
                     /translation="MSNEKPEARYQRLAADYRKRIAARLKADYGFRPDFVALLLIQDD
                     QEMLQLVREKEPEAWTEIAGLVTELKERLDEPPAYLIARYYQARASRLAAETICCQKR
                     SAVADFQLQLSRLMINPVTGLPILFLILYFGLYLFVGRFGAGVLVDLLEEHLFNQRLT
                     PWLTGASNRWLPWPALRELFTGEYGIITLGVRYAVALILPIVTTFFLAFAVLEDTGYL
                     PRLALLLDRFFKKIGLTGRAVIPLVLGLGCDTMATLVTRTLPTRRERFIATLLLALAV
                     PCAAQLGVIFSLLAEKPAGIAVWAMIILAVFLLAGFLASRLVPGDPPDFYLEIPLLRR
                     PLFSNILVKTGARIKWYFLEIMPMFIFASLLIWAGRLSHLFDLLVRILTFPVRWLGLP
                     AETAVAFLFGFFRRDYGAGGLYDLKKAGLLSGNQLLVAAVTLTLFLPCVAQFLMNIKE
                     RGWKVGVSISIFTLFFSFTVGFFLNLGLNLLGINL"
     gene            2426..2893
                     /locus_tag="PKN80_01820"
     CDS             2426..2893
                     /locus_tag="PKN80_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNQ34782.1"
                     /translation="MKCSFCGLEFDPKSAPSPCQRCPVSRWCGRRRCPGCGMEIAETG
                     SETGTGARRRRFGKGWCWGGPAASRIQTTKPAGAGEAVLTELERNRPAEVSSLRVHDR
                     SRLEKLMALGILPGLKITLLQKFPAYIVQVGNSQFALDEGLAACIFVKPLTED"
     gene            complement(3042..3710)
                     /locus_tag="PKN80_01825"
     CDS             complement(3042..3710)
                     /locus_tag="PKN80_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNQ34783.1"
                     /translation="MGIRENVREVLGGLPAGVELEAAVKSRSPEEIREAVAAGIRIVG
                     ANYVQEAAAVFPGLGPEVRRHFIGRLQKNKVKKAVELFDLVETVDSFELGAELARRAE
                     AAGKVMPVLVEVNSGREAQKAGVRPEAALELAAALAGLKGLRVEGLMTMGPAAAGPEE
                     LLACFRATRSLYEAGRERFGLSFRCLSMGMSDSYRLAVEAGANLVRLGTRLFGPRPSP
                     HLLK"
     gene            complement(3710..4636)
                     /locus_tag="PKN80_01830"
     CDS             complement(3710..4636)
                     /locus_tag="PKN80_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ34784.1"
                     /translation="MKYLFGPVPSRRLGYSLGIDIVPAKTCTLNCVYCQLGPTPEVTL
                     KRAPYLKTETLLAELSEFLKQPHRVDYLTFSGSGEPTLNSELGRMIRSAQKLTSKPVA
                     VITNGTLLYLEEVRRDLAAADLVLPSLDAVTPEAFVRVNRPHQGLTVDLVIQGLRDFG
                     REYRGEIWLEVMLVKGLNDSPEELEKLRETLRTLRVDRVQLNTVVRPPAEAEVRPLSR
                     EELESVARFLDCGAEIIVEFAREESAGFQADRQRVLETLRRRPLSRRDLVQLTGLHEH
                     EVVKILEGLLAARLITGERTGDREYYRAEPES"
     gene            4741..5253
                     /locus_tag="PKN80_01835"
     CDS             4741..5253
                     /locus_tag="PKN80_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNQ34785.1"
                     /translation="MKTVSIYSGPEAGDGQKYEALLKEHGYKLTPRRRAIIRLLGQGT
                     CATPERIWRRLKPGFGRLGLPTVYRNLEEFRKAGVAARVAGPEERFYYGLCRARRPDQ
                     HHHHIVCEKCRRVSEVSGCRLEGLAASVKKETGFQVTEHRLELVGLCRKCRTRTGKSK
                     PNTSSSREGK"
     gene            5250..6035
                     /locus_tag="PKN80_01840"
     CDS             5250..6035
                     /locus_tag="PKN80_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNQ34786.1"
                     /translation="MSLKNIKTATIGGFLAGMLIAFSAAPARAYHPLVTDDAGTLGRG
                     GWQLEVNGEFGRDREGAEKAVASELSATLGYGLGENLDLILGLPYLDQKLKTGGATVY
                     SEEGLGDLVLELKWLFHQSDGLALALKPTLTLPTGKEERGLGNGRPTGGLVFIATREL
                     NSGALHLNAGYLYNDNRLGDRKDIWHLSLAGEWAVTEKFKLVGNIGTERNPDPGDSQD
                     PAFFIAGFVYQVSERLSLDLGLKTGLNDAADDSAFLAGMTILF"
     gene            6061..7017
                     /locus_tag="PKN80_01845"
     CDS             6061..7017
                     /locus_tag="PKN80_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNQ34787.1"
                     /translation="MRKLRTLAWLLLAAGLLPALLAWKPAGREAGRAERSERTGRVRV
                     VATIPPLADFARQVGGNRVSVTLLLPPGATPHTFDPTPRQAAGLAEARLFVEVGAGLE
                     FWADKLVRAAGQPDLKRVVLAEGLPLIRETHHHEPGETDGAPGMGGIGNPHLWLDPVL
                     AGRMAARLTEALVEIDPDGVAYYRQREAAYQAELKRLDAEIAAAVGRFRTRAYVSFHP
                     AWTYFGRRYGLAEAGVIEAAAGREPTPAQLEAIVRSVKKYAIRAVFAEPQFSPRIAEA
                     VAENTGARVLFLDPLGNPEDPERDTYLELMRYNLKVMKTGLE"
     gene            7028..7816
                     /locus_tag="PKN80_01850"
     CDS             7028..7816
                     /locus_tag="PKN80_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNQ34788.1"
                     /translation="MGAEKILQVVDLRVVRNGLLALDGINLEMDRGAFLAIIGPNGGG
                     KTTFLKAILGQIPAAAGEIRILGWPPRLRQEKRPGAIGYVAQRSLLESDFPVSAFDVA
                     MMGRYGRLGPGRRAGRVDREKARECLALTGLAGLAGRPFRWLSGGQQQRVLIARSLAT
                     EPELLILDEPTTGVDAASQEEFYRLLKKLQRDLALSVILVSHDISLVPAYSDQVACLN
                     QRLHLHGKPAEVLRSEALQKAYGCQVDFLFHGEMPHRVVEKHDG"
     gene            7809..8600
                     /locus_tag="PKN80_01855"
     CDS             7809..8600
                     /locus_tag="PKN80_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNQ34789.1"
                     /translation="MGEMLSLLFMQRALAAGLLVSLLCGLLSVFVILKRLSFIGVGLS
                     HSAFAGVALGFLLGLDPGWTAVVYSVLVALLIGWIKQRGAVPEDTAIGIFFAANMALG
                     VLLIGLSRRYNLDLFGYLFGNILAITAADLRMIAIAGAAAALVLALFFKEFLFGAFDE
                     ETAAVMGVPTAAMNYLLLATMALVTVLAIKVVGIVLVSALLVIPGAIGRRLAANYRGM
                     ALISVLAGALASVGGLLLAYRLDLPAGAAIVLLATLFFGLSLLKR"
     gene            complement(8618..9289)
                     /locus_tag="PKN80_01860"
     CDS             complement(8618..9289)
                     /locus_tag="PKN80_01860"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ34790.1"
                     /translation="MIVLPGGAYHHHADHEAGAVARWLNSFGLQAFVLRYRLEPHPAP
                     LQDLQRAVRLVRHQAGCWRVNPDRVGVLGFSAGGHLAATLATHFDGGDPSAADPVERF
                     GCRPDAVILGYAGICFGRFRHHGSFQRLLGDNPTEAQVRDVSCDELVGPDTPPAFLWH
                     TAADASVPVNNSFCFAEALHRHGVPFELHVFPFGRHGLGLARELPEVARWTDLCRDWL
                     LRLGF"
     gene            complement(9333..10214)
                     /locus_tag="PKN80_01865"
     CDS             complement(9333..10214)
                     /locus_tag="PKN80_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNQ34791.1"
                     /translation="MSLFRRVAVFGVGLIGGSVGSGLKERGLVDEVVGVGRRSESLEA
                     ARARGLIDSVSLDPAAAFRGADLVLLAAPVAPVVELLESGLAAVPGRLLVTDACSTKA
                     AVLAAAGKLDPRHSFVGGHPIAGSEQRGSAAADPDLFRDRVTVLTPTRSTSADSLARV
                     RELWAALGSRVVEMDPRVHDRILALTSHLPHLLSVFACLAAAPALEAAGRDFFGSGFR
                     DISRLAAGSDEVWRDIFLTNRDNLVQDLETLVRLVSEWRSVLERGDAAAISAGLKEAA
                     LIRSRLLRKERGGASES"
     gene            complement(10222..10983)
                     /locus_tag="PKN80_01870"
     CDS             complement(10222..10983)
                     /locus_tag="PKN80_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNQ34792.1"
                     /translation="MGMWITLGVVILIILSNTIRVVQEYERGVIFRLGRLVGAKGPGL
                     FLLVPIVDRMVKISLRTVTFDVTPQEVITKDNVPVKVNAVVYFRVLEPSKAVVEVENY
                     QLATMQIAQTTLRGVLGQSELDELLSDREIINRRLQQIIDEQTDPWGIKVSIVELKEV
                     ELPETMKRAMARQAESERERRAKIITAEGEFQASEKILAASRVLEGSPLAIQLRYLQT
                     AAEISTEKNSTLVFPLPLDIISSLIKKPGETEKKG"
     gene            complement(11041..12255)
                     /locus_tag="PKN80_01875"
     CDS             complement(11041..12255)
                     /locus_tag="PKN80_01875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ34793.1"
                     /translation="MRRIDTETRALLAISLAFFFGFAGSGSIQSYLSPYLKGVLGVSP
                     LRSALVLTLVYITMAAGRVFSPVAIGRIGVGRLLFLGAVSFALFPLVFGFWTAYPLFL
                     VAAVLWGAGSSFFWTGGMAELLNHSRPDRYAANQGIMRLTQQVGQVGGFFLLGQLLAL
                     QSAAGVRAGFQVLFRTAALISLMMPFTLLLLPRAASDYHPISPFDILRAAVSRRRWVV
                     SLALFASGVSYGILLNLLNLFVSERFGVAWMNKTLVFFFIAAGLFSYLGGAFSDRFGR
                     REGFLLAFASGAFGTALLALVPTRPAAGLTALLLGVVFGVVPTVATGLVGDRTRPGER
                     VAGLAGVFLWRDLGAMVSIFSGGLLLQGAVSYRAIMWVFSAIFVLLCLLFGRADLTAG
                     GRISKLEEPARV"
     gene            complement(12264..12839)
                     /gene="pyrE"
                     /locus_tag="PKN80_01880"
     CDS             complement(12264..12839)
                     /gene="pyrE"
                     /locus_tag="PKN80_01880"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNQ34794.1"
                     /translation="MTQETVQRLFEETGALLSGHFLLSSGLHSDRYLQAALVLQAPGH
                     ALAFGRELAGRFRSEPVTAVVGPALGGIVIAFATAAGLPGTRALFTERENGRFTLRRG
                     FSLGPEDRVLVIEDIITTGGSTREVIELVAASGARVVGVGAIAERGAVPVDFGVRKET
                     LLRLVIESHPPETCPLCRQGLPLVKPGSRTR"
     gene            complement(12863..>13581)
                     /locus_tag="PKN80_01885"
     CDS             complement(12863..>13581)
                     /locus_tag="PKN80_01885"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNQ34795.1"
                     /translation="FVEKLAAGIQPAPADFSYRDDTGTFFQLWRLPADPRLEAFFRGR
                     RLYIADGHHRYQCNLRYGRAQRRPAAAYILTCFLNAAEVESQVNSFHRYLAAAGPDLL
                     RRLAGRFHLQPAAGAPELLAGLKPGWLGFGFAGRFYRFRIPARPGPEVVYLHKKILAG
                     LLDRSDESVAYEPDGLALAAGLDKPAGAAFFLPPVSIGRLHRIWEAGRILPRKSTYFY
                     PKVPSGLIVYRNDLEHFNRG"
BASE COUNT         2465 a         4480 c         4483 g         2153 t
ORIGIN      
        1 gaaaccgagg tactgcaaaa gttcctggcg gaccggcggc tgaaccgcag ccgggtgcgg
       61 gaagagatcc tggacgtttt tctgcgggcc gagcgccacc tgacggccga tcagctctac
      121 cagctggcca ggcggcgatt accctcggtg ggttacgcca ccgtttaccg gagcttgaag
      181 atcttctgcg actgcggact ctgccgcccg ataaaactgg agaacgaacc ggtccgctac
      241 gaacaccttt accggcacga ccatcacgac cacctggtct gcacccgctg cggccggctg
      301 gtcgaggtct ttgatccgac tctggaacag ctccaggaga aactcggccg aaaacacggt
      361 ttccagatgg agcgtcaccg gctggaatta tacgggatct gcccggaatg ccggaagaaa
      421 ggcgggccgg atgaccgaaa ttaacaacgt cagaaagaac gggaaaaggc cgttcgaagc
      481 cggtccgggc cggccgcggc tggccctggt gggcagcccc aatgtcggca agtcctcgct
      541 cttcaaccga ctcactaact cctacctgat cgtctccaat tatcccggta ccaccgtgga
      601 ggtcagccgg gggcaggccc ggatcggaga ggtggagttc gaggtgatcg ataccccggg
      661 cctctactcc ctgctgccgc tcaccgagga ggagcgggtg gcgcgcgaca ttctgaggct
      721 ggagcggccg gagctggtcc tgcacgtaat cgacgccaag aacctggaac ggatgctgcc
      781 cctgaccctc cagctgcggg aggccggcct ctccctggtg ctggtcctga acatcatgga
      841 tgacgccgag aagctgggcc tgcgcatcga cctggaacgc ctggaaaggg aactggggtt
      901 gccggcggcg gccaccgtct cgaccaccgg ccgcggcatt gacaagctga aggagatcat
      961 tttgaccgcc catgagcaac gaaaaacctg aggccaggta ccagcggctg gcggccgatt
     1021 accggaagcg gatcgcggcc cggctgaagg ccgattacgg attccggcca gatttcgtgg
     1081 ccctactcct gatccaggac gaccaggaga tgcttcagct ggtgcgggaa aaggaaccgg
     1141 aggcctggac cgaaatcgcc gggctggtga ccgaattgaa ggagcggctc gacgaaccgc
     1201 cggcctacct gatcgcccgc tactaccagg cccgggccag ccggctggcg gctgagacca
     1261 tctgctgcca gaaacgctcg gcggtggccg attttcagct ccagctcagc cggctgatga
     1321 tcaacccggt caccggcctg ccgatcctct tcctgatcct ttatttcggg ctctatctct
     1381 tcgtgggccg tttcggggcc ggcgtgctgg tggacctgct cgaggagcat ctcttcaacc
     1441 agcgcctcac gccctggctg accggggcat cgaaccgctg gctgccctgg ccggccctga
     1501 gggaactctt caccggcgaa tacggcatca tcaccctggg ggtgcgctac gcggtggccc
     1561 tgatcctgcc catcgtgacc accttcttcc tggccttcgc cgtgctggag gataccggct
     1621 atcttccccg gctggccctg ctgctggacc ggttcttcaa gaagatcggc ctgaccggcc
     1681 gggcggtgat cccgctggtg ctggggctgg gctgcgacac tatggccacc ctggtgaccc
     1741 ggaccctgcc gacccgccgg gagcgattca tcgccaccct gctgctggcc ctggccgtac
     1801 cctgcgccgc ccaactgggg gtgatcttct cgctgctggc cgaaaaaccg gccggcatcg
     1861 cggtctgggc gatgatcatc ctggccgtct tcctgctggc cggcttcctg gcctcccgtc
     1921 tggtaccggg cgatccgccc gatttttacc tggaaatccc gctgctgcgg cggccgctgt
     1981 tttccaacat cctggtcaag accggtgccc ggatcaagtg gtacttcctg gaaataatgc
     2041 cgatgttcat cttcgccagc ctgctcatct gggccggacg cctctcccac ctcttcgatc
     2101 tgctggtgcg gatcctgacc ttcccggtcc gctggctggg gctgccggcg gaaacggcgg
     2161 tcgccttcct cttcggtttc ttccgtcgcg actacggcgc cggcggcctt tacgatctca
     2221 agaaggccgg cctgctcagc ggcaaccagc tgctggtggc cgcagttacc ctgacccttt
     2281 tcctgccctg cgtcgcccag ttcctgatga acatcaagga gcggggctgg aaggtcggag
     2341 tctcgatctc gatcttcacc ctcttcttct ccttcacggt cgggttcttc ctcaatctcg
     2401 gcctgaacct gctggggatc aacctatgaa atgctcattc tgcggcctgg agttcgatcc
     2461 caaatcggcg ccgagcccct gccagcggtg cccggtttcc cgctggtgcg gccgccgccg
     2521 ctgccccggc tgcggcatgg aaatagccga aaccggttcg gaaacaggga ccggggcgcg
     2581 acggcggagg ttcggaaaag gctggtgctg gggcgggccg gcggccagcc ggattcaaac
     2641 aacgaaaccg gccggggccg gcgaagcagt cctgaccgag ctggaaagaa accggccggc
     2701 cgaggtatcc tcgctccggg tgcatgaccg gtcacggctg gaaaagctga tggccctggg
     2761 aatcctgccg ggattgaaga tcaccctgct ccagaagttc ccggcctaca ttgtccaggt
     2821 agggaattcg cagttcgcgc tcgacgaggg cctggcggcc tgtatcttcg tcaaaccgct
     2881 gacggaagac tgagtttcag aggcggaccg aaggtctcgc cggccttatc ccgaacggcg
     2941 gaccgaaggt ctcgccggcc ccacttcaaa aggcggaccg aaggtctcgc cgaacttatt
     3001 tcaaaaggcg gatcgaagta tctcttatgg ccgcgccgtc tttattttaa aaggtgggga
     3061 gacggccgcg gaccgaagag acgggtaccc aggcgcacca ggttggcccc ggcctcgacg
     3121 gccagccggt aggaatcgct catacccatc gagaggcatc gaaaggaaag tccgaaccgc
     3181 tcccggccgg cctcgtagag agaccgggtg gcccggaagc aggccagcag ctcctccggg
     3241 ccggcggccg ccggtcccat ggtcatcagc ccctccaccc gcagcccctt caaaccggcc
     3301 agggcggccg ccagttccag ggccgcctcg ggccggaccc cggccttctg cgcttcccgg
     3361 ccgctgttga cctcgaccag caccggcatc accttgccgg ccgcctcggc acgccgggcg
     3421 agctcggccc ccagctcgaa ggagtcaacc gtttcaacca ggtcaaagag ctcgaccgcc
     3481 ttcttcacct tgttcttctg aaggcggccg atgaaatggc gccgcacctc cggacccagg
     3541 ccgggaaaga cggcggccgc ctcctgcacg taattggcgc cgacgatccg gataccggcg
     3601 gccaccgcct cccggatttc ctccgggctt cgggatttca ccgccgcctc cagctcgacg
     3661 ccggccggca gcccgcccag cacctcccgc acgttctccc ggatacccat cacgactccg
     3721 gctcggcccg gtaatactcc cggtcgccgg tccgctcgcc ggtgatcagg cgggccgcca
     3781 gcaggccctc cagtatcttg acgacctcgt gctcgtggag gccggtaagc tgaaccaggt
     3841 cacgccggct caaggggcgg cggcgcaggg tctccagcac ccgctgccgg tcggcctgga
     3901 agccggccga ctcctcccgg gcgaattcga cgatgatctc ggccccgcaa tccaggaacc
     3961 gggcgaccga ttccagctcc tccctggaaa gcggccggac ctcggcctcg gccggcggcc
     4021 ggaccacggt gttgagctgg acccggtcga cccgaagggt ccgcagcgtc tcccgcagtt
     4081 tctccagctc ctcgggcgag tcgttcaggc ccttgaccag catcacctcc agccagatct
     4141 caccccggta ctcccggccg aaatcacgca acccctggat gaccagatcc accgtcaggc
     4201 cctgatgggg ccggttgacc cggacgaagg cctccggcgt caccgcgtcc agtgaaggaa
     4261 gaaccaggtc ggcggcggcc aggtcgcgcc ggacctcctc caggtaaagc agggtgccgt
     4321 tggtgataac cgccaccggt ttggaagtca atttctgcgc gctccggatc atccggccca
     4381 gctcggagtt gagggtcggc tcgcccgaac cggagaaggt caggtaatcg acgcggtgcg
     4441 gctgctttag aaactcggag agctcggcca gcagcgtttc ggtcttgaga tagggcgccc
     4501 gcttgagggt gacctccggg gtcggcccca gctggcagta gacgcagtta agggtgcagg
     4561 tcttggccgg tacgatgtcg atcccgaggg agtagccgag ccggcgcgag ggaaccggtc
     4621 caaagagata tttcatcagg cgctcctttc ctttgcggag aaaaccacta agacattata
     4681 caccaccggc cggccagccg gtttgacacc cgggggccgg gcggactaaa atcattataa
     4741 atgaaaaccg tttctattta ttcgggcccg gaggccgggg acggccagaa gtacgaggcc
     4801 ctgctgaagg agcacggcta caagctcacc ccgcggcgac gggccattat ccggctgctg
     4861 ggacagggaa cctgcgccac gcccgaacgg atctggcggc gtctgaaacc ggggttcggc
     4921 cgccttggac tgccgaccgt ctaccgcaac ctggaggagt tccgcaaggc gggcgtcgcc
     4981 gcccgggtgg ccggaccgga ggagcgtttc tactatgggc tctgccgggc ccggcggccg
     5041 gaccagcacc atcaccatat cgtctgcgaa aaatgccggc gggtcagcga ggtctccggg
     5101 tgccggctgg aagggctggc ggccagcgtg aagaaggaga ccggtttcca ggtgaccgag
     5161 caccggctgg aactggtcgg cctctgccgc aagtgccgga cgcgaaccgg aaaatcaaaa
     5221 ccaaacacca gttcatccag ggagggaaaa tgagcctgaa aaacatcaag accgcaacga
     5281 tcggcggatt tttggccggg atgttgatcg cgttttcggc cgcgccggcc agggcctatc
     5341 atcccctggt caccgacgac gccggcaccc ttggccgggg cggctggcag ctggaagtca
     5401 acggtgagtt tggacgcgac cgggagggag ccgagaaggc ggttgcctcg gaactgtcgg
     5461 ccaccctggg ttacgggctc ggcgaaaatc tcgacctcat cctgggcctt ccctatcttg
     5521 accagaaatt gaagaccggc ggcgccaccg tttacagcga ggaaggcctg ggcgacctcg
     5581 tcctggaact gaaatggctc ttccatcaat ccgacggcct ggccctggct ctcaagccaa
     5641 cccttaccct gcccaccgga aaagaggaac gcggtctggg caacggccgg cccaccggcg
     5701 gactcgtctt catcgccacc cgcgagctca actcaggcgc gctgcacctg aatgccggtt
     5761 acctttacaa cgacaaccgt ctcggagacc gcaaggacat ctggcatctc tctctggccg
     5821 gggaatgggc ggtaacggaa aagttcaagc tggtgggcaa catcggaacc gaaagaaatc
     5881 cagaccccgg cgacagccag gatccggcct tcttcatagc cggattcgtc taccaggtct
     5941 ccgaacgctt gagcctggac ctgggactga aaaccgggct gaacgacgcg gcggatgact
     6001 cggccttcct ggccggaatg acaatcctct tctgaccggc cgcggcgaaa caggatccgg
     6061 atgcggaaat taagaaccct ggcctggctg ctgctggcgg ccggactact gccggcgctg
     6121 ctcgcctgga agccggccgg ccgtgaagcc ggccgggcgg aaaggtcgga gcggaccggc
     6181 cgggtgcggg tggtcgccac cataccgccc ctggccgact tcgcccgaca ggtgggcggg
     6241 aatcgggtaa gcgtgaccct gctgctcccg ccgggagcca ccccccacac cttcgacccc
     6301 actccgcgcc aggcggccgg gctggccgaa gcccgcctct tcgtcgaggt cggcgccggc
     6361 ctggaattct gggccgacaa gctggtgcgg gccgccggcc agcccgactt aaaaagagtg
     6421 gtcctggccg agggccttcc ccttatccgg gagacgcacc accacgagcc cggcgaaacg
     6481 gacggcgcgc cgggaatggg cgggatcggc aatccccatc tctggctgga tccggtcctg
     6541 gccggacgga tggcggcgcg cctgaccgag gccctggtcg agatcgaccc ggacggagtg
     6601 gcctattacc ggcagcggga ggcggcctac caggcggaac tgaaaaggct ggacgccgag
     6661 atcgccgcgg ccgtcggccg ttttcgcacc cgggcctacg tttcctttca cccggcctgg
     6721 acttatttcg gccgccgcta cggacttgcg gaggccgggg tgatcgaggc cgcggccggc
     6781 cgggaaccga ccccggccca gctcgaggcg atcgtccggt cggtcaaaaa atacgcgatc
     6841 agggccgtct tcgccgaacc gcagttctcg ccccggatcg ccgaggcggt ggcggagaac
     6901 accggggccc gggtcctctt cctggatccg ctgggcaacc cggaggatcc ggagcgggac
     6961 acctatcttg aattgatgcg gtataatctg aaggtaatga agaccggact ggaataaggc
     7021 ctttgccatg ggcgcggaaa aaatactgca ggttgtcgat ctccgggtcg tccgaaacgg
     7081 cctgctggcc ctggacggga tcaacctgga gatggaccgg ggcgccttcc tggcgatcat
     7141 cggtcccaac gggggcggca aaacgacctt cctgaaggcc atcctgggac agattccggc
     7201 ggcggccgga gaaatccgga tcctgggctg gccgccccgg ctccggcagg agaagcggcc
     7261 cggcgccatc ggttatgtcg cccagcgctc cctgctggaa agcgatttcc cggtctcggc
     7321 cttcgacgtg gccatgatgg ggcgctacgg ccgcctggga cccggccggc gggccggccg
     7381 ggtcgaccgg gagaaggccc gcgaatgcct ggcgctgacc ggtctggccg gcctggccgg
     7441 ccggcccttc cgctggcttt cgggcggaca gcagcaaagg gtcctgatcg cccgcagcct
     7501 tgccaccgag ccggaactgc tgatactgga cgagccgacc accggcgtgg acgccgcctc
     7561 gcaggaggag ttttaccgcc tgctcaagaa gctgcagcgg gacctggccc tctccgtcat
     7621 cctggtttca cacgacatca gcctggtccc ggcctacagc gaccaggtgg cctgcctcaa
     7681 ccagcgcctc cacctgcacg gcaagccggc cgaggtgctg cgcagcgagg cgctgcaaaa
     7741 ggcctacgga tgccaggtgg actttctctt tcacggcgaa atgccgcacc gggtggtgga
     7801 gaagcacgat gggtgaaatg ctctcgctgc tcttcatgca aagagcgctg gcggccggat
     7861 tgctggtgag cctgctctgc gggctcctct cggtcttcgt gatcctgaaa cgcctctcct
     7921 tcatcggcgt cggcctctcc cactcggcct tcgccggggt ggcgctcggt tttctgctcg
     7981 ggttggatcc cggctggacg gcggttgttt actcggtgct ggtcgccctg ctcatcggct
     8041 ggatcaagca gcgcggcgcg gtcccggaag atacggccat cggcattttc ttcgcggcca
     8101 acatggccct gggcgtgctg ctgatcggcc tgagccggcg atacaacctg gacctcttcg
     8161 gctacctttt cggaaacatc ctggcgatca ccgccgccga tctccggatg atcgcgatcg
     8221 ccggcgcggc cgcggccctg gtcctggccc tcttcttcaa ggagtttctc ttcggcgcct
     8281 tcgacgagga gaccgccgcg gtgatgggcg tgccgacggc cgccatgaat tatctcctgc
     8341 tggcgaccat ggccctggtg acggtgctgg ccatcaaggt ggtggggata gtcctggttt
     8401 cggccctgct ggtgattccg ggcgcgatcg ggcggcggct ggcggccaac taccggggca
     8461 tggcgttgat ctcggtactg gccggcgcgt tggccagcgt cggcggcctg ctgctggcct
     8521 atcggctgga cctgccggcc ggcgccgcca tcgtactgct ggccaccctc ttcttcggcc
     8581 tttcgctttt gaagcgttag aaacgccccg ccccagctca aaaacccagg cgcaggagcc
     8641 aatcccggca gaggtccgtc cagcgcgcga cttccgggag ttcccgggcc aggcccaggc
     8701 cgtggcggcc gaagggaaag acgtgcagtt caaaggggac cccgtgccgg tgcagggcct
     8761 cggcaaaaca gaagctgtta ttgaccggga ccgaggcgtc ggcggcggtg tgccagagga
     8821 aggccggggg cgtatcggga ccgaccagct catcgcagga aacgtcgcgc acctgcgctt
     8881 cggtcgggtt gtcgcccagc aggcgctgaa aggaaccgtg gtggcggaag cggccgaaac
     8941 agatgcccgc gtaaccgagg atgaccgcgt ccggccggca gccgaagcgt tccaccgggt
     9001 cggcggcgga cgggtcgccg ccatcaaaat gggtggccag ggtggcggcc aggtgaccgc
     9061 cggccgagaa gcccagcacg ccgacccggt ccgggttgac acgccagcag ccggcctggt
     9121 gcctgaccag gcgcaccgcc cgctgaaggt cttgaagcgg ggccggatgc ggttccaacc
     9181 ggtagcgcag gacgaaggcc tgaagtccga aggagttgag ccagcgggcg accgccccgg
     9241 cctcgtggtc ggcgtggtga tggtaggcgc cgccgggcag aacgatcacc gcgccgcgcg
     9301 gcggcccggc cggacccgga taatattcca ggttaagatt cagaagcccc cccgcgctcc
     9361 ttccttaaaa ggcggcttcg gatcagcgcg gcctccttga ggccggcgct gatggcggcc
     9421 gcatcgccgc gctcgagaac cgaacgccat tcggagacca gccggaccag cgtttccagg
     9481 tcctggacca ggttgtcccg gttggtcaga aagatatccc gccagacctc gtccgatccg
     9541 gcggccagcc gggagatgtc ccggaaaccg ctgccgaaga aatcccggcc ggcggcctcg
     9601 agagccggcg cggcggccag gcaggcgaag accgaaagaa ggtgcggcag gtggctggtc
     9661 agggccagga tccggtcatg cacccgcggg tccatctcca ccacccggct gccgagcgcg
     9721 gcccacaatt cccggacccg ggccagcgaa tcggcggaag tggaccgggt gggcgtgagc
     9781 acggtgaccc gatcgcggaa aagatcggga tcggcggccg ccgaaccccg ctgctcggaa
     9841 ccggcaatgg gatggccgcc gacaaaggag tggcgcggat cgagcttccc ggccgcggcc
     9901 agcaccgccg ccttggtgct gcaagcgtcg gtgaccagga gtcggcccgg gacggcggcc
     9961 aggcccgact ccagcagttc caccaccggc gccaccggcg cggccagcag gaccagatcg
    10021 gccccgcgaa acgccgcagc cggatccagg ctgacggaat cgatcagccc gcgggcccgg
    10081 gccgcttcca gggactcgga acgacggccg accccgacca cctcgtcaac caggccgcgc
    10141 tctttcaatc ccgaaccaac cgaaccgccg atcaggccga cgccgaaaac cgcgaccctg
    10201 cgaaacaggg acatttaaaa ttcagccctt cttttccgtt tcgccgggtt tcttaatcag
    10261 ggatgaaatg atatccagcg gcagcggaaa gaccagcgtg gagttcttct cggtcgagat
    10321 ttcggcggcc gtctgcaggt agcgcagctg gatggccagc ggcgacccct ccagcacccg
    10381 ggaggcggcc aggatctttt cgctggcctg gaactcgcct tcggcggtaa tgatcttggc
    10441 ccggcgttcc cgttcgcttt cggcctggcg ggccatggcc cgtttcatcg tctccggcag
    10501 ctcaacctcc ttcaattcca cgatgctcac cttgatgccc cagggatcgg tctgctcgtc
    10561 gatgatctgc tggagccggc ggttgattat ctcgcggtcg gaaagaagtt cgtccagttc
    10621 cgactggccg agcacgccgc gcagggtggt ctgggcgatc tgcatggtcg ccagctggta
    10681 attctcgacc tcgaccaccg ccttggacgg ctcgagcacc cggaagtaga ccaccgcgtt
    10741 caccttgacc ggaacgttgt ccttggtgat cacttcctgc ggggtgacat cgaaggtcac
    10801 cgtgcgcagg ctgatcttga ccatgcggtc aacgatgggg accagcagga agagacccgg
    10861 acccttggct ccgaccaggc ggccgaggcg gaagatgacg ccgcgctcgt attcctggac
    10921 cacccggatg gtattggaaa gaatgatcaa aatcaccacg cccagcgtta tccacatgcc
    10981 cattacgcct ccttgtcttt caggtgtttc cgcagcgaaa aaaggacttt tccacggcca
    11041 ttaaacccgg gccggttctt ctaatttact aattcgcccg ccggctgtca aatcggcccg
    11101 gccgaagagc aggcaaagca ggacgaagat ggctgaaaaa acccacatga tggcccggta
    11161 ggagaccgcg ccctggagca ggaggccccc ggaaaagatg gagaccatgg cgcccaaatc
    11221 gcgccagaga aagaccccgg ccaggccggc cacccgctcg ccgggccggg tccggtcgcc
    11281 gaccagtccg gtggccacgg tcggaaccac gccgaagacc acgcccagca ggagggcggt
    11341 cagtccggcg gccggccggg tcggcaccag ggccagcagg gcggtgccga aggcgccgga
    11401 ggcgaaggcc agcaggaatc cctcccggcg gccgaaacgg tctgagaagg cgccgcccag
    11461 gtatgaaaag aggccggcgg cgatgaagaa gaagaccagg gtcttgttca tccaggcgac
    11521 cccgaaccgc tccgaaacga agaggttcag caggttgagc aggatgccgt aggagacgcc
    11581 cgaggcgaag agggccaggg agaccaccca gcggcggcgg gagacggcgg ccctgagtat
    11641 atcgaaaggc gaaatcgggt ggtaatccga ggccgccctg ggtagcagca ggagagtgaa
    11701 tggcatcatc aggctgatca gcgccgcggt ccgaaagagg acctggaaac cggcccggac
    11761 cccggcggcc gactgaaggg caagcagctg tccgagcagg aagaaaccgc ccacctgccc
    11821 gacctgctgg gtcaggcgca tgatcccctg gttggccgcg tagcggtcgg ggcggctgtg
    11881 gttgagcagt tcggccatcc cgccggtcca gaagaagctt gacccggccc cccagaggac
    11941 ggcggccacc aggaagagcg ggtaggcggt ccagaacccg aaaacaagtg gaaagagagc
    12001 gaaactcacg gcgcccagga agaggaggcg tccgacgccg atccggccga tggccaccgg
    12061 gctgaagacc cgacccgcgg ccatggtgat gtagacaagg gtcagcacca gggcgctgcg
    12121 cagcgggctg acgcccagca ctcccttcag gtaggggctc aggtaggact ggatggaacc
    12181 gctgccggcg aaaccgaaga agaaggccag cgaaatggcc agcagggccc tggtttcggt
    12241 gtcgatccgc cgcatgaacc gactcaacgg gtccggctgc ccggtttgac cagcggcagc
    12301 ccctgccggc agagcggaca ggtttcggga ggatggctct cgatcaccag gcgcagaagc
    12361 gtctccttgc ggacgccgaa atcaaccggc accgcccccc gctcggcgat ggcacccacc
    12421 ccgaccaccc gggcgccgct ggcggcgacc agttcgatca cctcgcgggt ggaaccaccg
    12481 gtggtaatga tatcttcgat caccagcacc cggtcttcgg ggcctagcga aaagccccgg
    12541 cgcagggtaa agcgtccgtt ctcccgctcg gtaaagagcg cccgcgttcc gggcagaccg
    12601 gccgccgtgg cgaaagcgat cacgatgccg cccagggccg gaccgaccac cgcggtaacc
    12661 ggctcggaac ggaaacggcc ggccagctcc cggccaaagg ccagggcatg tccgggagcc
    12721 tgcaacacca gcgccgcctg gaggtaacga tcgctgtgca gtccggagga gagaagaaaa
    12781 tggccggaga gaagcgcccc ggtctcctcg aagaggcgtt gaacggtttc ctgggtcatc
    12841 atctgttatc gtcctttcaa aactaacctc ggttgaagtg ctccaggtcg ttgcggtaca
    12901 cgatcaggcc gctggggacc ttgggataga agtaagtgct cttgcgcggc aggatccgtc
    12961 ccgcctccca gatgcggtgg aggcgcccga tggataccgg cggcaggaag aaggccgccc
    13021 cggccggctt gtccaggccg gcggccagcg ccagcccgtc gggctcgtaa gcaaccgatt
    13081 catcactccg gtccagcagg ccggccagta tttttttgtg caggtagacc acctcgggac
    13141 cgggccgggc gggaatccgg aagcggtaaa aacggccggc gaaaccgaag ccgagccagc
    13201 cgggcttaag gccggccagc aactccggcg ccccggccgc cggctgaagg tgaaagcggc
    13261 cggcgaggcg ccggagaaga tccggaccgg cggcggccag gtaacggtga aaggagttga
    13321 cctgcgattc tacctcggcc gcgttgagaa aacaggtcag gatataggcc gccgccggcc
    13381 gcctttgggc ccggccgtaa cgaaggttgc actggtaacg atggtggccg tcggcgatgt
    13441 agagacggcg gccgcggaag aaagcctcca gccgcggatc ggccggcagt cgccagagct
    13501 ggaagaacgt cccggtgtca tcgcggtagc tgaagtcggc cggggccggc tggataccgg
    13561 cggcgagttt ctcgacgaaa c
//
LOCUS       DAOQYN010000023        13149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_5229_length_13149_cov_4.047579, whole genome shotgun sequence.
ACCESSION   DAOQYN010000023 DAOQYN010000000
VERSION     DAOQYN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5229_length_13149_cov_4.047579"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..7383
                     /locus_tag="PKN80_01890"
     CDS             19..7383
                     /locus_tag="PKN80_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34796.1"
                     /translation="MPDADAGADRGDDFFVCIRTSAVITQGDLVQASITKGDVRFRST
                     AAYAGAGYASSVTAANVPTFITNLIGSGAAISKGGLGGSTPLAVFKIRSVGRDDTSTR
                     FQNLVIQVEDTDGDFAISDLMPFERDSSVSGLGLYLDNGDGIFDPAVDQPLNFESRPR
                     SISSFGEPYYRILCVLEDPEVITAAGKDFFVVIRSSAGIQAGDDFRLRIWGSNVRPLD
                     TTAVGYALQSASPAYFDAAVNGVAGDTLTLDTATLADGACSGWWVLLSKPGSFAEGKL
                     FPLSAVNGSDILSNGVNFAVEGVVVGDTVRLFRVDGPSFEKESSGLITCSGLVEPTIS
                     ITRPVAAGEAGVPPTFPVGYLATYSDSIAPNLTLYYDLDAVDTNYNSGPIGSITLPAG
                     ESGIEGSYAWNISGLPSGAYYVRGVLDDGVNPAVADYSDGVLTIGNPNPAILITSLEA
                     DRSGRTPAEVFVFDPAAGNLSINFRASGGVFDNEFYLSADASLDTGLDTYLGSVSGSG
                     DLSLFLAATGWAHGSYYIIGRRATGISQAYPVVADGKPLIDLTTPAGAPVVYPVNNEV
                     VIDFSATDADGDALVSLYYSPAPVFDAAAQLIVSDLASTDTTYTWPTGSVNPGVYYVY
                     GRIQDGNWQLLDGDQVDSAPALVEVLPRIVATGSVVDLTGSGQGILPGSGDTGLFGIN
                     ASSPENARIRELKIRLDNTDVDSSGLGTGDISFRLYWTFTDTGLVLPTEGPDIDLHVV
                     DPQGYHIYFGNPLAANGGELDADDLGAYGPGDGRGPENIYWPTGQAPSGQYEFYVDYW
                     NGYNPYGNVPVTLELRIRGQVVATYQDTLNGPGDDSIHWFYQYEPAPVVVPPFDPSML
                     SFGLFRDGGTSGYFDTATDTPLALDAYTWDYDPLTNSYIATLRLSDPAVLPLNDLGDN
                     TGYDFFLTVRTNPLMPDGQGFRVILDDVVLTDTTGSATQGGTLNAAPVDGHLIFSQAE
                     LRDLLAVISTSDYSIPAEMSEPGKDYLLKTDQALLLSRAEYADYDTGLNLDKFFTRPQ
                     VMPFNTAVPVFGFNLSGNAGVQTDPATYRPEAVSSLRVTFKSGGSFDPEIHLAALSDS
                     DPRGRGVVLYRDNGDGVFNVATDSPLVIDVLRGTSLPGYSWATGTDTKTLEIFLDTTY
                     GGALIPPRVGGNADYFLVLRTGSDNLLYGGQFKTYLRPGDLKFEVVPDQASQALSIEA
                     GSVQTSHLLFAASDLTSRYIDPQSELVSGSAAQSVHLPIIGLNLAKGTGMNVRLQGFK
                     VYLKGPGLELNDLLPVGGTSGGLQLWQDNKVGGRIGIFDTADPVVNLQSMSWFDEGTD
                     GAGNRVFSARVAPLISIDLYDNDTGSQRGDDLFVTLRLSSEADYGDTVLVELRDGEDG
                     VLFTTGSSAPGFAFLSDSDFEAPGINPIQVNVPVILSDISTGQLPSGGAPQGVAKLML
                     RSPESGSLYLRNLVVQIVPGTGFNLLGDLADIVTTVNSGLTLWQDNGDGVFSPDSDTF
                     LPLAAIENLSAIEEDYLRYRLTLLAPPAIPAAQDTAFFLAVRTTSNFSAGDSFQVRLW
                     SSNIEPERTRAFNFSDAAAADTCFSFQKIITRLLVGTGVLQPYLSIGIINPAAAGEEP
                     VRPDNTYNITWQPYREGPGVPTATVSLYYFDVGNPTDRFLIAAGLPYTTTSYLWSTRD
                     IPQGSYRIYAEIREGQAFASAISNGSIDLSDHAPAVTIVTPSQPRERAIYDTYTVRWQ
                     AFGLDSQNESLQLAYVDTAMVVRGTIATVNLNGYLNGSIYSTSWNTSAVPDGVYYIRG
                     VINDALWSDTGLSTGTVEVNHSVTPPARLTVTEPAGEILLDGGNDEELYPDPLHPQVL
                     TLGATLTPFTAADRVLLYDTWGVNVWDGDDAIWLDLDGSGTYSAGDLLLAGVAPHTGE
                     IGAPLSMVNPFVYNDANGNGVWTLGEHIYYEGGLGNQRFKYSNSQVDISWVPETPGAL
                     APTNTYIRIMLDRDGLAGSGDEIAVDTLLVPQGAPLAGSYTYDFSQLTLAEHGTYRVL
                     LQVNDVAGWYPGEYYFAPGPVTVNGAPQLAITTPDADTDVWNGYLYSINFNARDPDDN
                     ATVNLYLYDDISGDTYLISGNIREGTGLDTFVAWNPALTAAAPRNYYLQVRGTISDGN
                     WSSTVQAPGRVRILNTVPKVVLSGESNYNDITLRWYDQTPTGDVKRFILERWTQPDTG
                     LWQPIATGLAGDFNQIATGTYEFVDDNPNIYADRSLRSNYQVRVLVTTGTVTQTFISN
                     TFNALPLAFPLRPESFEVVPGTLQADLRWTWDYSTAPWVTGFIIERRPSYSNNFVTIG
                     MTNTHTFTDLNYNDSMLMVETDYQYRVRAYLTYPSGNVGYSAYSSTLEVEIVPTNNGN
                     IGVPNGGGGGGGCFIATAAFGSPLAREVVWLRRFRDRVLLRHGWGRAFVRFYYRHSPP
                     LAEFIRPRPFWCALVRAGLRPVIWLVRLLWIG"
     gene            7421..7867
                     /locus_tag="PKN80_01895"
     CDS             7421..7867
                     /locus_tag="PKN80_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34797.1"
                     /translation="MARFESNGLNAGILRRLAPVIFCFALAFTAVAAEGSFLAATPLS
                     NGLMAAGAAPDVDGGGDDAGVAIGDIGGGCFIATAAYGSPLAREVVCLSRFRDRVLQR
                     HLWGRAFVRFYYRHSPPLAGFIRPRPFWRALVRAGLRPVIWLARLA"
     gene            7923..10481
                     /locus_tag="PKN80_01900"
     CDS             7923..10481
                     /locus_tag="PKN80_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNQ34798.1"
                     /translation="MTRLSRVKPTVNAIKRLVLILFFAALGWTARTAEGSFIAGTPLT
                     TGLLPTAAVSGDFNNDGLLDLAVANYNGNTMTVFLGTGGGAFQPGVSYNTASGPTALA
                     AGHFNSDGLLDLAVANYNAFSVSVFMGVGDGTFQARMDFSTSRFPTGLAVNDYNRDNY
                     QDLAVASSGDGLVSIHRGNNNGTFQARVDFGAGTTPIGLVSGNFNADANPDLAVANFN
                     SNTVSVLIGNGNGTFQAKTDYAVGSQPRAILAADFNNDAIMDLVSANYGVNTVSVLRG
                     VGNGTFTSRVDYSTGSGPYYLAAADFNRDGNQDLAVSAYTGNSISLLLGTGTGLLQPR
                     TDYLVGTQPRGLATGDFNLDLQTDLAVANSGDDTAGILLNNFQAVIPTLVITGKVALI
                     GGPGAVTDVMITLSGDANSVVFPNLLGEYSFTVPSGGSYLVTPTLADYSFAPASRSYT
                     HITTGQSLQDYNGNHSSLSSYQVQGTVTKALDSSGFKGVEVALKFWTGTDTTIVDVTI
                     TDTFGNYTLNGFGALGGISYQVVPRAAGWAFTPAGTNLILVDADRTGVDFTAAEATYQ
                     ISGRVAVGTSASGLLDVRVNLAGRTLSGTLFNLNAYTDGAGNYQFNNIPAGDYNLTPQ
                     RTGWIFDPETRPLSIRENPFVNQDFGAYLLITLTYGPVGVPNPVDIGEEVECAVTAIL
                     TASDVPPGTLLNYTWRATSGYFDDSGTAISHLQNPIWIADLGLPPGFVDTGLVQYDLI
                     PIQVTVSCLNGIPVTSNFIEKVDLSSTVSVQSADDNCFIATAAFGSPLAREVNALRRF
                     RDTFLLRHGWGRAFVRFYYRHSPPLARFIRPRPFWCAVVRSGLRPVIWLVGHLV"
     gene            10555..11490
                     /locus_tag="PKN80_01905"
     CDS             10555..11490
                     /locus_tag="PKN80_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ34799.1"
                     /translation="MRIPAAGRFGLFCLFLCFQPAVAGAYWEWTPQTRRWINPKYEVK
                     ETAEAQYEWAETFVSARDYATAIQKLRKLIEIYPASDWAPRAQYRIGEIYERDGGLDK
                     AFEAYQKVVDNYPGSDRTTAAISRQFAIGRIMTRQRPGRLTFWKRDPSELLGSAVAAA
                     PYAAEAEQALFELGDYLFRRRRFQESVAHFDRLLGEYPESVLAPAAEFRAALATYRQA
                     EKQKNNDGLLLDAYGRFTACLDKYPDLRERPEAERYHRDIQSRLAEKILEAGRYYERN
                     GNRKAAAIYYRRVGAEYPGTPAAVQASEWLKKMEG"
     gene            11521..12042
                     /locus_tag="PKN80_01910"
     CDS             11521..12042
                     /locus_tag="PKN80_01910"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNQ34800.1"
                     /translation="MKNFSRTLKIASFIIGGFFLAAGCGYRNLSRPRLESLHLAAISN
                     QTMEPGVEIDLVRALGEEILRAGIRLEPGGAADWRLSGSITGYQRRPLTFKLEDPREV
                     SSYRLVLSVRFQLSGPASEASPGSRELEKTITVSRDFPLYGSAVRPEKEIVEWLTADL
                     AKRAVDWLAEVRP"
     gene            12039..12701
                     /locus_tag="PKN80_01915"
     CDS             12039..12701
                     /locus_tag="PKN80_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34801.1"
                     /translation="MTLQPFYLLLFQDLAAAERKAAELKKAAFPDGITAFNETVLYGE
                     DTTAGRIMELCQTPPFAGGRHFILLRRAEKLGKEDRDALFEYLKKPSASAFLVMLFET
                     SPKNKFPFPGNQFRFFFKGEAADAVDPFGVTRVLQRGNLKRALQILDRQYTDDRKHFP
                     QFMGILTWYLRSRVEGQGRLNNQSATLFKRFCELDRDVRTGRRSGREAVELAILHLAR
                     RL"
     gene            12720..>13149
                     /locus_tag="PKN80_01920"
     CDS             12720..>13149
                     /locus_tag="PKN80_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNQ34802.1"
                     /translation="MTYRQAIDFLEGRERFGIRLGLANMSRLLAALGEPQRGLKVFHV
                     AGTNGKGSTARLTAGLLQASGFRVGLYTSPHLLDTRERVQVDQRPVSRADFTRRIEEL
                     APVVERLSRFRPHSPTYFEVLTALAFRHFALEKVDFAVIEV"
BASE COUNT         2260 a         4411 c         4033 g         2445 t
ORIGIN      
        1 cccacctcgg ccctcgtgtt gcccgacgcc gacgccggcg cggaccgcgg cgatgatttc
       61 ttcgtctgca tccggaccag cgccgtcatc acccagggcg atctggttca ggcttcgatc
      121 accaagggcg acgtgcgttt ccgttcgacc gccgcttacg ccggggccgg ttatgcctcc
      181 tcggtcacgg ccgccaacgt cccgaccttc attaccaacc tgatcgggag cggcgccgcc
      241 atttccaagg gcggcctcgg cggcagcacg ccgctggcgg tcttcaagat ccgctcggtc
      301 ggacgcgacg ataccagcac ccgtttccag aacctggtga tccaggtcga ggataccgac
      361 ggcgacttcg ccatctcgga cctgatgccg ttcgagcggg acagctcggt cagcggcctg
      421 ggcctctacc tggacaacgg cgacggcatc tttgacccgg cggtcgacca gccgttgaac
      481 ttcgagtcgc ggccgcgttc gatcagcagt ttcggagaac cttactaccg gattctctgc
      541 gtcctggaag acccggaggt catcaccgcc gccgggaagg atttcttcgt ggtgatccgg
      601 agcagcgccg gcatccaggc cggggacgat ttccgcctcc gcatctgggg gagcaacgtg
      661 cggccgctgg ataccacggc cgtcggctac gcgctgcaat cggcgtcgcc ggcttacttt
      721 gacgccgccg tcaacggcgt ggccggggac accctgaccc tggacaccgc gacgctcgcc
      781 gacggggcct gttccggctg gtgggtgctg ttgagcaagc cgggttcctt cgccgaagga
      841 aagctcttcc cgctcagcgc ggtcaacggc agtgacatcc tgtccaacgg cgttaatttc
      901 gcggtcgaag gcgtggtggt cggcgacacg gtccgcctct tccgggttga cgggccgagc
      961 ttcgagaagg agagcagcgg cctgatcacc tgctccgggc tggtggaacc gaccatctcc
     1021 atcacccggc cggtggccgc cggcgaggcg ggcgttccgc ccaccttccc ggtcggttac
     1081 ctggccacct actcggacag catcgcgccg aacctgacgc tttactacga tcttgatgct
     1141 gttgacacca actacaacag cggcccgatc ggctcgatca cgctgccggc cggcgagtcc
     1201 ggcatcgagg ggagttacgc ctggaacatc agcgggctgc ccagcggcgc ctactatgtc
     1261 cggggcgtcc tcgatgacgg ggtcaatccg gcggtggccg attactccga cggcgtcctg
     1321 acgatcggta atccgaaccc ggccatcctc ataacctcgc tcgaggccga ccggagcggc
     1381 cggaccccgg ccgaagtctt cgtcttcgac ccggcggccg gcaacctgag catcaacttc
     1441 cgggccagcg gcggcgtctt tgacaatgag ttctacctga gcgccgacgc cagcctggac
     1501 accggcctgg atacttacct gggcagcgtc tccggttccg gtgacctgtc ccttttcctg
     1561 gccgcgaccg gctgggcgca cggcagttac tacatcatcg gccgccgggc gaccgggatc
     1621 agccaggcct acccggtggt tgccgacggc aaaccgttga ttgacctgac cactccggcc
     1681 ggcgccccgg tggtttaccc ggtgaacaat gaggtggtca tcgacttctc ggccacggat
     1741 gccgatggcg acgcgctggt ttccctctac tacagcccgg cgccggtctt tgacgcggcg
     1801 gcccagctga tcgtttccga tctggcttcg accgacacca cctacacctg gccgaccggc
     1861 agcgtcaacc ccggcgttta ctatgtttac gggcgcattc aggatggcaa ctggcagttg
     1921 cttgacggcg accaggttga cagcgcgccg gctctggtcg aggtcctgcc caggatcgtc
     1981 gccaccggca gcgtggttga tcttaccggc agcggccagg gtatcctgcc cggcagcggc
     2041 gacaccggtc tcttcgggat caacgccagt tcgcccgaaa atgcccggat ccgggaactg
     2101 aagatccggc ttgacaatac cgatgtcgac agcagcgggc tgggtaccgg cgacatctcc
     2161 ttccggctct actggacctt taccgacacc ggtctggtcc tgccgaccga ggggccggat
     2221 atcgacctgc acgtggttga tccgcagggt taccacatct acttcgggaa tccgctggcc
     2281 gccaacggcg gcgaacttga tgccgacgac ctgggcgctt acggacccgg cgacggccgc
     2341 ggtcccgaga acatctactg gccgaccggc caggcgccga gcggacagta tgagttctac
     2401 gtcgattact ggaatggcta caacccgtac ggcaacgttc cggtaactct ggaactgcgc
     2461 atccgcggcc aggtggtggc cacttaccag gacaccctga acggtcccgg cgacgattcc
     2521 atccactggt tctatcagta cgagccggcg ccggtcgtgg tgccgccttt cgatccttcc
     2581 atgctcagtt tcggcctctt ccgggacggc ggcaccagcg gttacttcga caccgcgacc
     2641 gacacgccgc tggccctgga cgcctacacc tgggattacg acccgctcac caacagctac
     2701 atcgccaccc tgcggttgag cgacccggcg gtcctgccgc tcaacgacct gggcgacaat
     2761 accggctatg acttcttcct gaccgtccgg accaacccgt tgatgccgga cgggcagggt
     2821 ttccgggtca tcctggacga cgtggttctt accgatacca ccggttcggc cacccagggc
     2881 ggcaccctca acgccgcacc ggtcgacggc cacctgatct tctcgcaggc ggagctgcgg
     2941 gacctgctgg cggtgatcag cacctccgat tactcgattc cggccgagat gtccgagccc
     3001 ggcaaggatt acctgctcaa gaccgaccag gccctgctgc tttcccgggc cgaatacgcc
     3061 gactacgaca ccgggctcaa cctggacaag ttcttcaccc ggccgcaggt gatgcccttc
     3121 aacacggcgg tgccggtctt cggattcaac ctctccggca acgccggcgt ccagaccgat
     3181 ccggccacct atcggccgga ggccgtctcg tcgctgcggg tgacctttaa gagcggcggc
     3241 agtttcgatc ccgagattca cctggcggcg ctttccgaca gcgacccgcg cggccgtggg
     3301 gtggtcctgt accgcgacaa cggtgacggc gtcttcaacg tggcgaccga ctcgccgctg
     3361 gtgattgacg tcctgcgcgg aaccagcctg cccggttaca gctgggcgac tgggaccgac
     3421 accaagacgc tggagatttt ccttgacacc acttatggcg gtgccctgat tccgccgcgg
     3481 gtgggcggca acgccgatta cttcctggtc ctgaggaccg ggagcgacaa cctgctttac
     3541 ggcggccagt tcaagaccta cctccggccg ggcgacctga agtttgaggt tgttccggat
     3601 caggccagcc aggccttgag catcgaggcc gggagcgtcc agaccagcca cctgctcttc
     3661 gcggccagcg acctgacctc ccggtacatc gatccgcagt ccgaactggt cagcggcagc
     3721 gcggcccagt cggtccacct gccgattatc ggcctcaacc tggccaaggg gacgggcatg
     3781 aatgtccgcc tccagggctt caaggtttac ctgaaggggc cgggattgga attgaacgac
     3841 ctgctgccgg tcggaggcac ctccggcggg ctccagttgt ggcaggataa caaggtcggc
     3901 ggccggatcg gtatcttcga tacggccgat ccggtggtga acctccagtc aatgagttgg
     3961 ttcgacgagg gtaccgacgg cgccggcaac cgggtctttt cggcccgggt cgccccgttg
     4021 atctcgatcg acctgtacga taacgacacc ggcagccagc gcggcgacga tctgtttgtc
     4081 accctgcgcc tcagcagcga ggccgattac ggcgacacgg tcctggtcga actgcgcgac
     4141 ggcgaggacg gggtgctctt taccaccggc agctcggcgc ccggcttcgc cttcctgagc
     4201 gacagcgatt tcgaggcgcc cgggataaac ccgatccagg tcaacgtgcc ggtcatcctc
     4261 tcggatatca gcaccgggca gctgcccagc ggcggcgctc cgcagggagt ggccaagctg
     4321 atgctgcgca gcccggaaag cggttcgctc tatctgcgca acctggtggt ccagatcgtg
     4381 ccgggcaccg gcttcaacct gctgggcgac ctggccgaca tcgtcacgac cgtcaacagc
     4441 ggtctgacgc tctggcagga taacggcgac ggagtcttca gcccggacag cgataccttc
     4501 ctgccgctgg ccgcgatcga gaatctgtcg gccatcgagg aagattacct gcgctaccgg
     4561 ctgacgctgc tggcgccgcc ggccataccc gcggcccagg ataccgcctt cttcctggcg
     4621 gtccggacca cgtccaactt cagcgccggc gactccttcc aggtgcgcct ctggtccagc
     4681 aacatcgagc cggaacggac ccgggccttc aacttcagcg atgccgcggc cgccgacacc
     4741 tgcttctcct tccagaagat cattacccgg ctcctggtgg gcaccggcgt gctccagccg
     4801 tacctgtcaa tcgggattat caatccggcc gccgccggag aagagccggt ccggccggat
     4861 aacacctaca acatcacctg gcagccctac cgggaaggtc cgggagtgcc gacggccacc
     4921 gtgtcgctct actacttcga cgtgggcaat ccgaccgacc gtttcctgat cgccgccggg
     4981 ttgccttata ccacgaccag ttatctctgg tcgacccggg acattccgca ggggagttac
     5041 cgcatctacg ccgagatccg cgaagggcag gccttcgcct cggcgatcag caacggcagc
     5101 atagacctca gcgaccatgc gccggcggtt accatcgtga cgcccagcca gccgcgtgaa
     5161 cgggccattt acgacaccta cacggtgcgc tggcaggcct tcggactgga cagccagaac
     5221 gagtccctgc agctggctta cgtcgatacg gccatggtgg tgcgcggcac gatcgccacg
     5281 gtcaatctta acgggtacct gaacggaagc atctactcga ccagctggaa taccagcgcg
     5341 gtgccggacg gcgtctacta catccggggc gtgatcaacg acgcgctctg gagcgacacc
     5401 ggcctctcaa ccgggaccgt cgaggtcaac cattcggtga cgccgccggc ccggctgacc
     5461 gtgaccgagc cggccgggga gattctcctc gacggcggaa acgacgagga gctctatccc
     5521 gatccgctgc atccgcaggt cctgacgctg ggcgccaccc tgacgccttt cacggcggcc
     5581 gaccgggtgc ttctgtacga cacctggggg gtcaacgtct gggacggcga cgacgccatc
     5641 tggctggacc tggatggcag cggcacctat tcggccggag atctcctgct ggccggagtc
     5701 gccccgcaca ccggtgaaat cggcgcgccc ctgagcatgg ttaacccgtt cgtttataat
     5761 gacgcgaacg gaaacggcgt ctggacgctg ggcgaacaca tctactacga gggcggtctc
     5821 ggcaaccagc gattcaagta ttccaacagc caggttgaca tcagctgggt tcccgagacg
     5881 ccgggcgccc tggcaccgac caacacctat atccggatca tgctcgaccg cgacggcctg
     5941 gccggtagcg gcgacgagat cgcggtggat accctgctgg taccgcaggg ggctccgctg
     6001 gccggcagct acacctatga tttcagccag ctgaccctgg cggaacacgg tacctaccgg
     6061 gtcctgctcc aggtcaatga cgtggccggc tggtacccgg gcgaatacta cttcgcgccc
     6121 ggcccggtga ccgtcaacgg ggcgccgcag ctggccatca cgactccgga tgccgacacc
     6181 gacgtctgga acggctatct ctacagcatc aacttcaatg cccgcgaccc ggacgacaac
     6241 gccacggtca acctctacct gtacgacgat atttccggcg atacttacct gatctccggg
     6301 aacatccggg aggggaccgg tctggatacc ttcgtcgcct ggaacccggc cttgaccgcc
     6361 gcggcgccgc gcaactacta cctgcaggtg cgcgggacca tcagcgacgg gaactggagc
     6421 agcacggtcc aggcgcccgg ccgggtccgc atcctcaata ccgtcccaaa ggtggtcctg
     6481 agcggcgaat ccaactacaa cgatatcacc ctgcgctggt acgaccagac gccgacgggg
     6541 gatgtcaagc ggtttatcct cgaacgctgg acccagccgg ataccgggct ctggcagccg
     6601 atcgccaccg gcctggccgg ggatttcaac cagatcgcca ccggcaccta cgagtttgtc
     6661 gatgacaatc cgaacatcta cgccgaccgc tcgctgcgca gcaactacca ggttcgggtg
     6721 ctggtcacca ccggtaccgt tacccagacc ttcatctcga acacgtttaa cgccctgccg
     6781 ctggccttcc cgctgcggcc ggaatccttc gaggtggtgc cgggcacgct gcaggcggac
     6841 ctccgctgga cctgggacta ctcgacggcc ccgtgggtga ccggtttcat catcgagcgc
     6901 cggcccagtt acagcaataa cttcgtgacg atcggcatga ccaataccca taccttcacc
     6961 gatctgaatt acaacgactc gatgctgatg gtcgagaccg attaccagta ccgggttcgg
     7021 gcctacctga cctacccgag cggcaatgtc ggttactcgg cctacagttc cacgctggag
     7081 gtcgagatcg tgccgaccaa caacgggaac atcggcgttc ccaatggcgg cggcggtggc
     7141 ggcggctgct tcatcgccac ggccgccttc ggttccccgc tggcccggga ggtggtctgg
     7201 ctccgccggt tccgcgaccg ggtcctgctg cggcacggct gggggcgggc cttcgtccgc
     7261 ttctattacc gccattcacc gccgctggcc gagtttattc gaccccggcc cttctggtgc
     7321 gccctggtgc gcgccgggct gcggccggtc atctggctgg ttcgcctgct ctggatcggt
     7381 tgagaccggg ttgaacgggg ggatctccga ggagaaaagc atggcgcgct ttgagagcaa
     7441 cgggttaaac gccggtattc tcagacgcct ggccccggtc attttctgct ttgccctggc
     7501 ctttacggcc gtggcggccg aggggtcgtt cctggccgcc accccgctgt caaacgggct
     7561 gatggcggcc ggagccgctc cggacgttga cggcggcgga gacgacgccg gggtcgccat
     7621 cggggatatc gggggcggct gctttatcgc cacggccgct tacggctctc cgctggcccg
     7681 ggaggtggtc tgcttgagcc ggttccgcga ccgggttctg cagcgccatc tctgggggcg
     7741 ggccttcgtc cgcttctatt accgccattc accgccgctg gccgggttca tccggccccg
     7801 gcccttctgg cgcgccctgg tgcgcgccgg attgaggccg gtcatctggc tggctcgact
     7861 ggcttgaatt gggcctctcc ctgggaaggg cagcctttga tcgccaacaa gaggaggtaa
     7921 tcgtgactcg gctgagtaga gtaaaaccga ccgtcaatgc catcaagcgc ctggtcctga
     7981 tcctgttttt tgcggccctg ggatggaccg cccggacggc cgaggggtcg ttcattgccg
     8041 ggactccgct gacgaccggt ctcctgccga ccgcggcggt cagcggggat ttcaacaatg
     8101 acgggctgct ggacctggcc gtggccaact acaacggcaa cacgatgacg gtcttcctcg
     8161 gtacgggcgg cggcgctttt cagccgggcg tcagttacaa caccgccagc ggtccgaccg
     8221 ccctggcggc cggccacttt aattcggacg ggctgctgga cctggccgtg gccaactaca
     8281 atgccttttc ggtgagcgtt ttcatgggag tgggcgacgg cacctttcag gcccggatgg
     8341 atttcagcac cagccgtttt ccgaccgggt tggccgttaa cgactacaat cgcgataatt
     8401 accaggacct cgcggtggcc agttccggcg atggcctggt gagcattcac cggggcaaca
     8461 acaacggcac ttttcaagcc cgggtggatt tcggcgccgg caccaccccg atcggcctgg
     8521 taagcggcaa cttcaacgcc gacgccaacc cggatctggc ggtggccaat ttcaacagca
     8581 ataccgtcag cgttctgatc ggcaacggaa acgggacctt ccaggccaag accgattatg
     8641 cggtcggcag ccagccgcgg gccatcctgg ccgccgattt caacaatgac gccattatgg
     8701 acctggtttc ggccaactat ggcgtcaaca cggtttcggt gctccggggc gtcggaaacg
     8761 ggaccttcac cagccgggtg gattattcca ccggcagcgg tccctattac ctggcggccg
     8821 cggatttcaa ccgcgatggg aaccaggacc tggccgtatc cgcctacacc ggcaactcga
     8881 tcagtctgct tctgggtacc ggaaccggcc ttttacagcc ccggaccgac tacctggtgg
     8941 gaacccagcc gcgcggcctg gccacgggcg atttcaacct ggaccttcag accgacctgg
     9001 cggtggccaa cagcggggac gacaccgccg gcattctgct taacaatttc caggccgtca
     9061 ttccgaccct ggtcatcacc ggcaaggtgg ccctgatcgg cgggcccggt gcggtcaccg
     9121 acgtgatgat caccctcagc ggggatgcca actcggtggt ctttccgaac ctgctggggg
     9181 aatacagctt cacggttccc agcggtggca gctacctggt gaccccgacc ctggccgact
     9241 acagcttcgc tcccgccagc cggagctata cccacattac caccggccaa tccctgcagg
     9301 attacaacgg aaaccacagt tcgctctcca gctaccaggt gcagggaacg gttaccaagg
     9361 ccctggattc atccgggttc aagggagtgg aggtggccct caagttctgg accggtaccg
     9421 ataccacgat cgttgacgtc accatcaccg acaccttcgg caattacacc cttaacggtt
     9481 tcggggccct gggcggcatc agttaccagg tggttccccg ggcggccggg tgggccttca
     9541 ccccggccgg gaccaacctg atcctggttg acgccgaccg gaccggcgtt gacttcaccg
     9601 ccgccgaggc cacttaccag atttccggtc gggtggcggt cggtaccagc gccagcggac
     9661 tgctcgacgt ccgggtcaac ctggccggcc ggaccctttc cgggacgctt tttaacctga
     9721 acgcctatac ggatggcgcc ggcaattacc agttcaacaa cataccggcc ggcgattaca
     9781 acctgacccc gcagcggacc ggctggatat ttgatcccga aacccggccg ctgtcgatcc
     9841 gggagaatcc tttcgtcaac caggatttcg gcgcctacct tctgatcacc ctgacttacg
     9901 gcccggttgg ggttcccaac ccggttgata tcggagagga ggtcgagtgc gccgttacgg
     9961 ccatcctcac cgcctccgac gttccccccg gcacgctgct caattacacc tggcgggcca
    10021 cgtccggtta cttcgacgac agcggaacgg cgatcagtca cctgcagaat ccgatctgga
    10081 tcgccgacct gggcctgccg ccgggcttcg ttgacaccgg gctggtccag tatgacctga
    10141 ttcccatcca ggtgaccgtt tcctgcctga acggcattcc ggttaccagc aactttattg
    10201 aaaaggtcga tctttcctcg actgtttccg tccagtcggc cgacgacaat tgttttatcg
    10261 ccacggccgc tttcggctcg ccgctggccc gcgaggtcaa tgcgctgcga cgtttccggg
    10321 acaccttcct gctgcgtcac ggctgggggc gggcctttgt ccgcttctat taccgccatt
    10381 cgccgccgct ggcccggttt attcggcccc ggcccttctg gtgcgccgtg gtccgttccg
    10441 gcctgcggcc ggtaatctgg ctggttggcc acctggtctg accggccggt tggccgcgat
    10501 cgttgttgcc gatgccgggc gtaagcatta aaatccatta acgggataac ccacatgagg
    10561 attccagccg ccggtcgttt tggcctcttt tgcctctttc tctgctttca gcctgcggtt
    10621 gcgggcgcgt actgggaatg gacaccccag acccgccgct ggatcaaccc gaagtacgag
    10681 gtgaaagaga cggccgaggc ccagtatgaa tgggccgaga ccttcgtttc cgcccgggat
    10741 tacgccaccg ccatccagaa attgcggaaa ctgatcgaga tttacccggc cagcgactgg
    10801 gccccccggg cccagtaccg gatcggcgag atctacgagc gggatggcgg cctggacaag
    10861 gctttcgaag cctaccagaa ggtggttgat aattatccgg gatcagatcg gacaacggcg
    10921 gccatcagcc ggcagttcgc catcgggcgg atcatgaccc gccagcggcc cggacggctg
    10981 accttctgga aacgggatcc ttccgaactg cttggttcgg cggtggcggc ggccccttac
    11041 gcggctgaag ccgaacaggc gctcttcgaa ctgggcgatt accttttccg ccgtcgccgt
    11101 tttcaggagt ccgtggccca cttcgaccgg ctgcttggtg agtatccgga gagcgtcctg
    11161 gccccggccg ccgagttccg ggccgcgctg gccacttacc ggcaggccga aaagcagaag
    11221 aataacgacg gacttctact ggatgcctac ggccgtttta ccgcctgcct ggacaagtac
    11281 ccggatctcc gggagcggcc ggaggcggaa cgctaccacc gcgatattca gtcgcgcctg
    11341 gcggaaaaga tcctggaggc cggccgttat tacgagagga acggcaaccg gaaggcggcc
    11401 gccatctact accgtcgggt cggggcggag tatcccggaa cgccggccgc ggtacaggcc
    11461 tctgaatggc tgaaaaagat ggaaggttaa tcagttttaa gccgccctgc ttgctcccag
    11521 atgaaaaatt tttcccggac actgaaaatc gcgtccttta ttatcggcgg attcttcctg
    11581 gcggccggct gcggctaccg gaatctttcc cggccccggc tggaatccct tcacctggcg
    11641 gccatctcca accagaccat ggaaccgggg gtcgaaattg acctggtccg ggccctgggc
    11701 gaagagatct tgagggccgg gatccggctg gaaccggggg gggcggccga ctggaggctc
    11761 tccggaagca ttaccgggta ccagcgccgg ccgcttacct tcaagctcga agaccccagg
    11821 gaggtcagca gttaccggct tgttttgagc gtccgttttc agctttccgg accggcttcc
    11881 gaggcttcgc ccgggtcccg ggaactggaa aagacgatta cggtaagccg cgattttccc
    11941 ctttacggct ccgcggtccg cccggaaaag gagatcgtcg agtggttgac cgccgatctt
    12001 gccaaacggg ccgtggactg gctggccgaa gtccggccat gacgctccaa cctttctacc
    12061 tgctgctttt tcaggacctg gccgccgccg agcggaaggc ggccgaactg aaaaaagcgg
    12121 ccttcccgga tggcatcacc gccttcaacg aaacggtcct ctacggtgag gataccactg
    12181 ccggccgaat catggagctt tgccagacgc ctcccttcgc cggcggccgc catttcatcc
    12241 tgctccggcg ggccgagaag ctcggcaagg aggaccggga tgcccttttc gaatacctta
    12301 agaaaccctc ggccagcgcc ttcctggtga tgctctttga aaccagcccc aaaaacaagt
    12361 ttcccttccc cgggaaccag ttccgtttct tcttcaaggg tgaggcggcc gatgccgtgg
    12421 atcctttcgg ggtcaccagg gtcctccagc gcgggaatct gaaacgggcc cttcaaattc
    12481 ttgacaggca gtacacggac gatcggaagc attttcctca attcatgggt attctgacct
    12541 ggtatttgcg tagccgggtg gaaggccagg gtcgtcttaa caaccagtcg gccaccctct
    12601 tcaagcgttt ctgcgaactg gaccgcgatg tccggaccgg ccgccggtcc ggccgggaag
    12661 cggtcgagct ggccatcctt cacctcgccc ggcgtctttg agcgggcccg cctttagcaa
    12721 tgacttaccg tcaggcgatc gattttctgg aggggcgcga gcgtttcggg atacggctgg
    12781 gattggccaa catgtcccgt ctgctggccg ctctcggcga gccccagcga ggcctgaagg
    12841 ttttccacgt ggccgggacc aacggcaaag ggtcgaccgc ccgcctgacg gccggtcttc
    12901 ttcaggcctc cgggttccgg gtcggtcttt acacctcccc gcacctgctg gacacccggg
    12961 agcgcgtcca ggtcgatcag cgtccggtca gccgggccga tttcacccgt cggatcgagg
    13021 aactggcccc ggtggtcgaa cgtctttccc ggttccggcc gcacagtccg acctacttcg
    13081 aggtgctcac cgccctggcc ttccgccatt tcgccctgga aaaagtggac ttcgcggtga
    13141 tcgaggtcg
//
LOCUS       DAOQYN010000024        13090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_5256_length_13090_cov_4.913387, whole genome shotgun sequence.
ACCESSION   DAOQYN010000024 DAOQYN010000000
VERSION     DAOQYN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13090
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5256_length_13090_cov_4.913387"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="PKN80_01925"
     CDS             <1..1525
                     /locus_tag="PKN80_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ34803.1"
                     /translation="SRLAAGKGGPGIKPRGTETGRGPVFRGRKQSHPFHRQAGHHQGI
                     LDTGDKKMRKIGLLLVFLLLWSGSGAADQVILKTGTVWEGKVIKETAESLLFQTDKSQ
                     ITIPRERIKEIIIHTSFFYAEKAFEAKNYAEALRLYREYLKKYPAGEDREPAAFKAGL
                     CYLKMADPEPALVSFEEFLKTYPDSAFSTEAALHIGEAYFAGNRKEKAGEYFQKALAS
                     ASKSVAGSAAYFLFRLWEDKSPAETLEFCENYLKEHPRSERTPEVIYLKAEVLYSRAV
                     QRSQADQNISDYQAITAMLEETAGQHPGAAIPENILKLRMDCYERQARYAQKHKTLKK
                     YADLVAQGDKEKAARMVRDAADKLITEGEVTEAITLYRVIKQEYPETALAPEVCFQIA
                     KILQEEANKHGAMEYNEHEILIQKYPKTQYAEEACFDLVKQHQRIGKYPQAIERLNYF
                     LKNYPESKYVQNGTFLLGFLYHQAKQKEEARKVWEWYLKKYPDDITYSSIIKAYLGG"
     gene            2077..2967
                     /locus_tag="PKN80_01930"
     CDS             2077..2967
                     /locus_tag="PKN80_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34804.1"
                     /translation="MKKNNLLYKVFLMIIGINLFVIVSQAPSQTINKELLLKGVLYSQ
                     SLIKDAQCRYVSQNFFKNDWRTILEMEWAYKPGKSFLKGKAYGYLEGKKELVEDIAYI
                     FNGVNGYSIGYENGKVIGVTMQNRERVEKLFGKVPPEALFLRFWPLGIELQQILSDPA
                     LKLLPDRESVEGSSCYVAHLESVTGSLNPQNGYKIWLDPEKGFLPRKIEIYYSGKLKT
                     VLGPITLSNFGNNLWFPTGAKITTFSPDFPQGNIGRVIYEDIRINQNLGDEKFTPVFE
                     PGTKIYDSALGISYTIPAAK"
     gene            3503..4507
                     /locus_tag="PKN80_01935"
     CDS             3503..4507
                     /locus_tag="PKN80_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34805.1"
                     /translation="MNGSILWNKKRFIKLIIFLLFLNVVGLFNGWKTPQLAAETSSEI
                     LEIITQGVAYNDSLIKDISFHYTIDYRISEEWLKYQSNRTLSQGNLPAGMVVRDPTRE
                     PVIFSGILKAEGDKFFITSTVKAVGDGKILQDKIISFDGITLIRLNLIQDYGDISQGL
                     EFMGRNSIWDPRSFQSFFLDGESLYKKMADKSVDKVFVGKETIDGFPCYCLEVNIPYS
                     NEEGSFVAKKKIWIDPQSGFRVRKTLAFDRKMGRPLTATWFTLKKFGDVWYPIKVKFE
                     SEGQPNLPLGPHIIMTLDVNNVSINQNNFGKNTFIADLNKVSRITDFTHGGVTFKTQK
                     "
     gene            4566..5963
                     /locus_tag="PKN80_01940"
     CDS             4566..5963
                     /locus_tag="PKN80_01940"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNQ34806.1"
                     /translation="MKFNLGLSVPYLIFLFFSSALFLFSQTTHDPLCGPKSLQALFHK
                     LGVESSLEEVSRLSGYREKEGTTLLGLYQAARKKGLYAAGVKMGLEEIADLKTPVIVH
                     FWDNHFLMVEGDGTDTLIITDPPNPPQSISREQFQPFYSGFALLVSKRKISLPDTETK
                     KPDIRFEGYTHDFGKVDPGRKIEHIFTFKNAGEKTLTISRVRTTCGCTAALVSEKSIP
                     PNGRGEIRVNFDTSGRSGFQIQKIYVQTNDPVTPLVQLQIQGLLKADLAVSPGNISFG
                     EVKKGTAADREIYVVDRTGENIQITRVESSSPLLSATAAPITNKPYQGTKILVSLSPA
                     APLGSLEGKVTIYTTDKKHPRVEIPVAAKVVGEIEVYPSMFFFGLVKQGETPTAGITI
                     STTGTASLKIKSIEPPRSFISTELIPVSEGREYRLTAGLGKDAPKGTLKEKITIYTDN
                     PDQPKIEVPVYGLVE"
     gene            6096..7097
                     /locus_tag="PKN80_01945"
     CDS             6096..7097
                     /locus_tag="PKN80_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34807.1"
                     /translation="MRREKWMVVFLVGAVSFVLMGPHITIAATQNELLQLVKMRSEIL
                     KEGKVSLRVFRYYWKNENDYKTFTEKLKVFNTTPPNQFLKWLSEEKGISLSKAMLVGN
                     ENQTYFFKGNSDHLISDWNFDEIDYVFVEKKGEYLFRPTIPERIIKITHSEYSTNGST
                     ICAYRTVNSKGIMNIGSRISYFKVSNVDLALLYYKQLPEANISLNFIAENNKCIYQST
                     EVNDMKLQLIFDVSLAGVIPYLKIAEKNGKILRETISGAFSKVNDNIHLPFFTSTTIP
                     TTNVFLKDVVLEQTLYIIDNWELRPVTSEELLIHPLKNTTVSDQTFGEPTLNYPYIP"
     gene            7891..9510
                     /locus_tag="PKN80_01950"
     CDS             7891..9510
                     /locus_tag="PKN80_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34808.1"
                     /translation="MASKPDRIKRPGSGLPVFLFGLAWLLLPPLAAGIHAQEKPALPV
                     VRLWVFASKDCGDCRAVQPEALKGLEEKLRCGIEARYFDIGDIASYALLTKFEEKYGD
                     TDNDPPVLFIGREVIGGEKEVADRLEKAIVRYREAGGCPWPDGVSFPPPGEGRMSAAK
                     PEETKPGRPAGVVSEKAGEKPAPIYLAFFHQYGCRECQRVFHLIRYVEKKHPAVVVRE
                     FDLSRKENQEINEAIGRRFNVPEERRLLTATLFIGAECLQGREITLERIEARIARYAA
                     TGSPCPWEIGAEDLAAGRRSALDRFRSFGPAAVAAAGAIDGINPCAFTTVVFLVSYLA
                     FIGRRRREILLAGISFTLAVFLTYFLVGGGLFAFLRRLPARSLLPVLLTAAVGGLALG
                     LGVLSFLDFLKARRGDFRGIALQLPEPVKGRIRLTISREMRRRGLLPAAFAAGFAVSV
                     LELACTGQVYLPTITLIAGREEMRRAGLAYLLLYNAAFIMPLAAVFLAAYFGAASQRL
                     ADFARRRVAAVKLLTAVFFLVLGTLLLASLR"
     gene            9595..9711
                     /gene="rrf"
                     /locus_tag="PKN80_01955"
     rRNA            9595..9711
                     /gene="rrf"
                     /locus_tag="PKN80_01955"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            9928..11853
                     /locus_tag="PKN80_01960"
     CDS             9928..11853
                     /locus_tag="PKN80_01960"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNQ34809.1"
                     /translation="MGRIRNLGFFGQGKSGKTSLVEALLFKAGSINRLGSVTDGNTTT
                     DFDPEETKRQLSISLALGHLDWKGVRINLIDTPGYADFAGEAVSGVSAADFAVICVPA
                     DSGPSVGTDNAWKLAAASGLPTFIFVTKSETGPDAAEAIAGRLSEKWKVRVTPWTNRE
                     ALTESAAEADDATLNKYLESGQLGEAEFHTGIRQVLLTRALVPLFVGSAAKGEGLEAF
                     LDFVCEYLPEYEELPPVSLAGGAETSRRLGSEPVMLHVFKTASDPFVGRLSYFKVIRG
                     TLAPNSTLYNQNRSEKERVGQVLYVQGKKQEPVEKVQPGDLLAVAKLNYTHTGDTLVA
                     VGQNGHLPALVFPQPAISVSLVAKEKGMEDKLGSAFSRIMEEDQTIQIARDAETSETI
                     LSGLGDLQLEIWISRLANRFGVEVVKGVPKIAYRETVTGTASGQGRFKRQTGGHGQYG
                     DCWIKVEPLPRGSGFEFVDAIVGGAIPRQYIPSVEKGVREAMTKGPLANYPMTDLRVT
                     LYDGTFHPVDSSDIAFQIAGSLALKKVGLEARPVLLEPIVNVEILVPKENVGDIIGDI
                     NGRRGKVLNMDSVDNEYQKISAQMPLAEMSNYATTLRSITSGRASYQAQFSHYELVPY
                     QISEKIIKERQVKKEED"
     gene            11856..12398
                     /locus_tag="PKN80_01965"
     CDS             11856..12398
                     /locus_tag="PKN80_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HNQ34810.1"
                     /translation="MVEVRIKGIVAEKASGRNILVLEDGAKRLLSVTIGVTEAQAIIL
                     SLKKLGVPQPLVYDLLVKLAGELGARPLRIEVLDRSGDLLITQLVYQAGDRVRFLACR
                     PGDGVALALRCQVPIFVHENLMKMVTLTADTRRKRPKPPPAPMTEAEVDRIKTEIENL
                     SAEEFWRRVSSRTEGESGGS"
     gene            12400..>13090
                     /locus_tag="PKN80_01970"
     CDS             12400..>13090
                     /locus_tag="PKN80_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ34811.1"
                     /translation="MSGHSKWASIKHKKAAVDAKRGKAFTKVIREIMMAARGGGANPD
                     NNPRLRLAIDKARDLNMPADNIKRAIQKATGEGMENQLEEVSYEGYGPAGVAIKLEVV
                     TDNKRRTAAEIRSIFTKQNGSLGEVGCVSWLFSRKGQLTVPRAGVDEDKLISLALDAG
                     AEDVASETDNYLVYTRAEDMDKVRKALEDNQIKVGSGTLSMLPKSSVKVEGKEAEQLL
                     KLLDALEDQEDV"
BASE COUNT         3221 a         3272 c         3639 g         2958 t
ORIGIN      
        1 ttcccgactg gctgcgggta aaggaggtcc aggaattaag ccccgaggga ctgaaaccgg
       61 ccgcggccca gtattccggg gaaggaagca atctcaccct ttccatagac aggctggaca
      121 tcaccaggga attcttgata ctggtgacaa gaaaatgaga aagatcggtc ttcttttagt
      181 tttcttattg ctgtggtccg gttccggcgc cgccgaccag gttatcctga agaccggcac
      241 cgtctgggag ggaaaggtga tcaaggaaac cgccgagagc cttcttttcc agaccgacaa
      301 aagccagatc accatcccca gggagcgaat caaggagatc attatccaca cgtccttctt
      361 ctacgccgag aaggccttcg aggcgaagaa ttacgccgag gccctccggc tctaccggga
      421 gtacttgaag aagtatccgg cgggagaaga tagggagccg gcggccttca aagcgggcct
      481 atgttatctg aagatggccg acccggagcc ggccctggtt tccttcgagg agttcctgaa
      541 aacctatccc gacagcgcct tctccaccga agcggccctg catatcggag aggcgtattt
      601 tgccgggaac cggaaggaga aggccggaga gtattttcag aaggcgctgg cctcagcctc
      661 caagagtgtg gctggaagcg ccgcttactt tctatttcgc ctgtgggagg ataaatcgcc
      721 ggccgagacc ctggagttct gcgagaatta cctgaaagag catccgcgga gcgaaaggac
      781 cccggaggtc atctatctga aagcggaggt gctgtacagc cgggcggtcc agcggagcca
      841 ggcggaccag aacatctccg attatcaagc gataacggca atgctcgagg agaccgccgg
      901 tcagcatccc ggggccgcga taccggaaaa catactcaaa ttgcggatgg attgttacga
      961 gaggcaggcc aggtacgccc agaaacataa aacgctgaaa aaatacgcgg acctggtcgc
     1021 ccaaggggat aaagaaaagg cggcccggat ggtcagggac gcggcggata aattaatcac
     1081 ggagggggag gtaactgaag ccattactct ttaccgggta atcaaacagg aatatccgga
     1141 gacggccctc gccccagagg tctgtttcca gatagcgaaa atacttcagg aagaggctaa
     1201 taaacatggc gcgatggaat ataacgagca cgagatttta atccaaaaat acccgaagac
     1261 ccaatatgca gaagaagcat gttttgattt agtaaaacaa catcaacgca taggaaaata
     1321 tccccaagcg atagaacgtt taaattattt tctaaaaaat tatccggaaa gcaaatatgt
     1381 tcaaaatggc acctttctcc ttggatttct gtatcaccaa gcgaaacaaa aggaagaagc
     1441 aagaaaggtg tgggaatggt accttaaaaa atacccggat gacatcactt attcgtcaat
     1501 tataaaagca tatttaggag ggtagaaagc tggtttatga agtgtctccc gtttttagac
     1561 aggcaggttc attatataac attatcttaa aaaggagatg cgggtatgaa gaataaattg
     1621 atttttgttg ttttgggggt attggtgttt atatttggat cattgaaacc gccgctaagt
     1681 tctgccgcaa caaccttgtc agaaaaagac atgaatgcgt tttttggtgg ggcttgtaat
     1741 caacggtgtg tggaatatga agattgcgcg gtagcgcttg atattgtaaa ttgtgccggg
     1801 cggccttcat gtggtgggag ttgcctcgct tgttcatcgg gaggcagaag aagagggatg
     1861 tgtgaaactc acgccggtag ccagtgtact caaacgacac cttcgtgtgg ttggtcgagg
     1921 gctggtacgt gtaatagggt tacagatttg gtttgcactt gcctcgaagg agaagatcag
     1981 aatccaattt ttgtatcgtg cggcaattac tatcgttgcg aatgattttc gcttaaaatt
     2041 caagtgttga atgtgtttat attacgggag atgaagatga aaaaaaacaa tttgctgtac
     2101 aaggtttttt tgatgataat cggcataaat ttgtttgtca tcgtttcgca ggccccctct
     2161 caaacgatca ataaagaatt gctgttgaaa ggcgtccttt attcccaatc tcttattaaa
     2221 gacgcccagt gtcgttatgt ttctcagaat tttttcaaga atgattggcg aaccatcctg
     2281 gaaatggaat gggcctataa accgggaaag tcttttctga aagggaaggc ctacgggtat
     2341 ttagaaggca aaaaagaact tgttgaagat attgcctata ttttcaacgg agtgaatggt
     2401 tactcgatag gatacgaaaa tggcaaagtg atcggggtga ccatgcagaa ccgggaaaga
     2461 gtcgagaagc tctttggcaa ggtaccgccc gaggctttat tcctgcgttt ttggccgtta
     2521 ggtattgaat tacaacaaat cctgtccgat cccgctttga aattgcttcc cgatagagag
     2581 agcgtcgagg gaagcagttg ttatgtcgcg catcttgaaa gcgtaaccgg atctcttaac
     2641 cctcaaaatg gttacaaaat ttggttggat ccggagaaag gttttttgcc ccgcaaaata
     2701 gagatttatt actcaggcaa actgaaaaca gtgctggggc cgataactct gtcgaatttc
     2761 ggaaataatc tctggtttcc tacgggggcg aaaattacca cgttttcacc tgattttccc
     2821 cagggtaaca tcggtcgcgt catttatgaa gatattagaa tcaatcaaaa tctcggtgat
     2881 gaaaaattca ccccggtttt tgaaccggga accaaaatct atgattcggc tctgggcata
     2941 tcatacacca ttccagcggc taaataatat ccgggaaata ttggggggcg gtgttgctcg
     3001 cgtaaaaaat aaacttaagg tggaaacatg agaagaacca agattttgtt aatgattata
     3061 atgctatttg ggggtaatga aacggttaaa acaagagtcg ccttttctgc tgaattatta
     3121 actcatgaag agatggtttt gtttgttggt aaagtttgtg gtcccgtcac ctgttcaaca
     3181 aatggaagct atcggtgttc ttgttgttat tgtggcgttt ttaattggga agaatgcagc
     3241 gcttacaaaa catatgatca atgtataagt agttctagtg gttcatgttc ggatagttat
     3301 cgactatgtg aaaacatggg atcaaattac tcggatcgac aagttgttcc aggtcgaaaa
     3361 tgtgacggta aggatttttg ttcaacaaca aattggaaag gtgtaaatta tacaggatcg
     3421 gtttattgct atgcagtata cacgggatgc aattagaatt aaaaacatag aaaattgaac
     3481 tgtcacaaat taggaggaaa aaatgaacgg tagcatctta tggaataaga aacggttcat
     3541 taaattaatt atatttttgc tgtttcttaa tgttgtagga ttgtttaatg gttggaaaac
     3601 ccctcaattg gcggcggaga caagtagtga gattttagag attattaccc agggtgttgc
     3661 ttacaacgat agcctaatta aggatatttc cttccattat actattgatt atagaatctc
     3721 agaagaatgg ttaaaatatc aatcaaaccg aacattatct caaggaaacc ttccagctgg
     3781 gatggtggtg agagatccga caagggaacc ggtgattttt tcaggaattc ttaaagcaga
     3841 aggcgataag tttttcatta cttcaactgt gaaagcggtt ggagacggga aaattttaca
     3901 ggataaaatc atcagttttg atggcataac attaataagg ttgaatttaa ttcaagatta
     3961 tggagatatt tctcaaggtt tggaattcat gggacggaat tccatttggg atccgaggag
     4021 ttttcaatca ttttttttag atggcgaatc cctttacaaa aaaatggcgg ataaaagtgt
     4081 tgataaggtt tttgtgggca aagaaactat tgacggattt ccttgctatt gtctggaagt
     4141 gaatattccc tactcaaacg aagaaggtag ttttgtagct aaaaaaaaga tctggatcga
     4201 tcctcaaagc ggtttcaggg taaggaaaac attggctttt gatcgaaaaa tgggaagacc
     4261 gttaacagct acgtggttta ctcttaaaaa atttggagat gtctggtatc ccataaaagt
     4321 gaaattcgaa tcagaaggtc aacctaattt gccgcttggt ccacatatca taatgacgct
     4381 ggatgtaaat aatgtttcaa taaaccaaaa caatttcggg aaaaacacat ttattgccga
     4441 tctaaataaa gtaagcagaa taacggattt tactcacggc ggtgttactt ttaaaaccca
     4501 aaaataatct gggcggataa aaaaggtttt ttgtttttaa ccgggaaaac ttttggggag
     4561 gtttcatgaa atttaacttg ggtttatcgg tgccgtatct gatttttttg tttttttctt
     4621 ccgctctctt tcttttttcg caaactaccc acgacccgct ctgcggccct aagagccttc
     4681 aggcgctctt ccataaattg ggcgtggaat cctcgcttga ggaggtctcc cgtctctccg
     4741 gctaccggga gaaggagggt accaccctgc tgggccttta ccaggccgcc agaaagaaag
     4801 gcctttacgc ggccggggta aagatgggcc tggaggaaat tgccgacctt aaaaccccgg
     4861 tcatcgttca cttctgggac aaccatttcc tgatggtgga aggggacggc accgacaccc
     4921 tgataatcac cgatcctccg aacccacccc aatccatatc cagggagcaa ttccagccct
     4981 tctactccgg ctttgccctc cttgtttcca aacgcaaaat ctccctgccc gacacggaaa
     5041 ccaaaaaacc ggacatccgt ttcgaagggt atactcacga tttcggaaag gttgatcccg
     5101 gccggaagat agagcacatc tttactttca agaacgccgg cgagaagacg ctgaccatct
     5161 cccgggtcag gaccacctgc ggctgcaccg ccgccctcgt ttccgagaaa agcatcccgc
     5221 cgaatggccg gggagagatc cgggtgaatt tcgacacttc gggaagaagc ggcttccaga
     5281 tacagaaaat ctacgtgcag accaacgatc cggttacccc cctggttcag cttcaaattc
     5341 aaggcctgct taaagccgac ctggccgtct ctccaggaaa tatttcattc ggcgaggtca
     5401 aaaaagggac cgccgccgac cgggaaatat acgtcgtgga ccgaaccggc gagaacatac
     5461 aaatcaccag ggtggaatca tcctccccgc tcctttccgc caccgccgcc ccgattacca
     5521 acaaacccta ccagggtact aaaatccttg tctccctttc ccccgcggcc ccgctgggaa
     5581 gcctggaagg gaaggtgacc atatatacca ccgacaaaaa acaccccaga gtggagattc
     5641 cggtggccgc caaggtcgtc ggcgagatag aggtttatcc ttcgatgttt ttcttcggcc
     5701 ttgtcaagca aggagaaacc ccgaccgccg ggatcaccat ttccaccacc gggacggcct
     5761 ccttaaagat aaaaagcata gaacctcccc ggagtttcat ctcgaccgag ctcatcccgg
     5821 tctcggaggg gagggagtac cggctgacgg ccggcctggg caaggatgcg cccaaaggca
     5881 ctctgaaaga gaaaataacc atttacaccg acaaccccga tcagccgaag atagaggtgc
     5941 cggtgtacgg tctggtggaa taggctggcg gttttgtacc tcctcgatgt tttttgatcg
     6001 ccatagaaaa tgggttgtga aaggagtgga gtcggggaaa agagactcga tgtcgatttt
     6061 aaatagagta gaacgttggg gaaatggagg atgaaatgag aagagaaaaa tggatggttg
     6121 tgtttctggt aggtgccgtt tcttttgtgc ttatggggcc acacatcaca atcgctgcta
     6181 cgcaaaatga acttttgcag ttagtaaaga tgcgcagcga aatacttaaa gaaggaaagg
     6241 tttccctgag agtttttcgg tactattgga agaatgaaaa tgattataaa acgttcactg
     6301 aaaaattaaa agtttttaac actacgcctc cgaaccaatt cttgaaatgg ctttcagaag
     6361 aaaagggcat atctttatca aaagctatgc tggtgggcaa cgaaaatcaa acatatttct
     6421 tcaagggtaa tagcgaccac ttaattagtg attggaattt cgatgaaatt gattatgttt
     6481 ttgttgaaaa aaaaggagaa tatttattta gacctacaat acctgaaaga attattaaaa
     6541 taacacattc cgaatatagt acaaacggaa gtactatatg cgcatataga acagtaaatt
     6601 caaaaggaat aatgaatatt ggttcacgta taagttattt taaagtttcc aatgtagatc
     6661 tagccttatt gtattataaa cagttgccag aggcaaatat ttcattgaat ttcattgctg
     6721 agaacaataa atgcatttac caaagcacgg aagtaaatga catgaaatta caactaattt
     6781 ttgatgtttc tttggcggga gtaataccgt atctaaaaat tgcagaaaaa aacggtaaaa
     6841 ttctacggga aactatttct ggcgcatttt caaaagtaaa cgataatatt catcttccat
     6901 tttttacttc tacaacaatt cccacaacta atgtattttt gaaagatgtg gtgctcgaac
     6961 aaacattgta tatcattgat aattgggaac tcaggccggt taccagtgag gaattgttga
     7021 ttcacccgct taaaaatact accgtttcgg atcaaacatt tggagaaccg actctgaatt
     7081 acccgtatat tccctaaaat cctcttttaa cttagcgagg gaatacaatg actgcaagaa
     7141 ttttaaagta cgcaaagatt tttttaattc tggtgttttg ggttggaatt ttaagtttta
     7201 gtacgaatct ttccgggatg gaagatcaaa gataccttat attgtatgat ctggaaaaaa
     7261 taagcggcgg ggtttgcagt aaccatggtt attgcgtagg ttcttttagg tgtatcggac
     7321 aaggccgttg taaaatagtg ttattaattt tctgcgttgc cgatccagtt tgtagtggga
     7381 cctatccccc gtatagttca agaaaatgcg aaggaatcgg aaatcctcag atttgccatg
     7441 agggtttgaa atatatgctt attcagtcct gcaaagatga gtgcgcattg ttcccagagt
     7501 gtggctgtag tagcaattgg agcggcgatg gatggtttgg ttatttgcag gattgttggt
     7561 attgaattat gcgactaatg ttttagtctc cacgagaatt aataaacacg tcgatgccat
     7621 tatattaaag ataggcctat agttctagtg tagtgtccct aacgcttctt tacaatgatt
     7681 gattttggtt actatctggt ccaccgacgc tgtccagacg aaaggttttg gatttttgtt
     7741 attttcggcg atgtagtggt caatggcggc aaccagttcc tgaacgcttc ggaacgttcc
     7801 gcgccgggat ctcgtttttt ttcagtagaa accctgagcg tctttattac agccgtcggt
     7861 ttacaattgc atctggagat ggtgtagaag atggcctcca agcccgacag aataaagcgg
     7921 ccggggagcg ggcttcccgt cttcctcttc ggcctggcct ggctcctgct gccgccgctt
     7981 gcggccggga ttcacgccca ggaaaaaccc gccctgcccg tcgtccgact ctgggtcttc
     8041 gcctcgaagg attgcggcga ttgccgggcg gtccagccgg aggcgctgaa gggcctggaa
     8101 gaaaaactgc ggtgcggaat cgaggccagg tatttcgata tcggggatat cgccagctac
     8161 gcgctgctca cgaagttcga ggagaagtac ggcgataccg acaacgaccc gccggtcctt
     8221 tttatcggcc gggaggtcat cggcggcgag aaggaggtgg ccgaccggct ggagaaggcg
     8281 atcgttaggt accgggaggc gggcggctgc ccctggccag acggggtctc ctttccgccg
     8341 ccgggtgaag gaagaatgtc ggcggcaaag ccggaggaaa cgaaacctgg ccgcccggcc
     8401 ggagtcgttt cagagaaggc gggggagaaa ccggcgccaa tctacctggc ctttttccac
     8461 cagtacggct gccgggagtg ccaacgggtc ttccacctga taaggtacgt cgaaaagaag
     8521 cacccggcgg tggtggttcg ggaattcgac ctaagccgga aggagaacca ggagatcaac
     8581 gaggcgatcg gccgccggtt caacgtgccg gaagagcggc gtcttttgac ggcgaccctc
     8641 ttcatcggcg ccgaatgcct ccaggggcgg gagatcaccc tcgagaggat cgaggcgcgg
     8701 atcgcccggt acgcggccac cggcagcccc tgcccctggg agatcggggc ggaggatctg
     8761 gccgccggcc ggcggtcggc gctggaccgg ttccgctcct ttggtccggc ggcggtggcg
     8821 gccgccggcg cgattgacgg gatcaatccc tgcgccttca ccaccgtcgt cttcctggtc
     8881 tcttacctgg ccttcatcgg ccggcggcgc cgggagattc tcctggccgg gatctccttc
     8941 acgctggcgg tcttcctgac ctacttcctg gtcggcggcg gcctcttcgc cttcctgcgc
     9001 cggctgccgg cccgctccct gctcccggtt ctgctgacgg cggccgtcgg cgggctggcg
     9061 ctcggcctgg gggttttaag ttttctcgac tttctcaagg cgaggcgggg cgacttccgg
     9121 gggatcgcgc tccagctgcc ggagccggtc aagggccgga tccggctgac gatcagccgg
     9181 gagatgcgcc gccggggcct tctcccggcg gcctttgcgg ccggcttcgc ggtttcggtc
     9241 ctggaactgg cctgcacggg ccaggtctac ctgccgacga tcaccctgat cgccggccgg
     9301 gaggagatga ggcgggcggg cctggcctac ctgctgctct acaacgcggc gttcatcatg
     9361 cccctggcgg cggtcttcct ggccgcctat ttcggggccg cctcccagcg gctggccgat
     9421 ttcgcccggc gccgggtggc cgccgtgaag ttgctcaccg ccgtcttctt cctcgtcctg
     9481 ggaacgctgc tgctggccag cctccggtag cggacccggc gcccgggccc ggtttggccg
     9541 gggccgtcag cggatttgac ttgcccggcc ggcccatgat acaattttta cgcttctggt
     9601 gatcatagcg gaagggaaac acccgttccc atttcgaaca cggccgttaa gccttccagc
     9661 gccgatggta ctgctccggc agcggagtgg gagagtaggt cgtcaccagg ggcatttttt
     9721 tttgcccgtc ccccggattt tcaggaccct cccgccgccc cccaggcgcc ggggccggtt
     9781 tgcctcgacg gccccgttaa gatataataa gtaaataaat caaccccctg gtgaaatttc
     9841 gtgaagcctc ttctttccgc gcttttctga cggccgattg gcggcactcg gaatttcccc
     9901 gaacgatcga atcttcagga gcaaaggatg ggcagaatcc ggaatcttgg tttcttcggt
     9961 cagggcaaga gcggcaaaac cagcctggtg gaggccctgc ttttcaaggc cggctcgatt
    10021 aaccgcctgg gcagcgtcac cgacggcaat accaccacgg atttcgatcc ggaagagacc
    10081 aagcgccagc tgagcatcag cctggccctc ggccacctcg actggaaggg ggtccggatc
    10141 aacctgatcg acacgcccgg ctacgccgat tttgccggcg aggcggtctc cggggtgtcg
    10201 gcggccgatt tcgcggtgat ctgcgtgccg gccgacagcg gtccctcggt gggcaccgac
    10261 aatgcctgga aactggccgc ggccagcggg ctgccgactt ttattttcgt taccaagagc
    10321 gagaccggcc ccgacgccgc cgaagcgatc gccggccggc tgagcgaaaa gtggaaggtc
    10381 cgggtcacgc cctggacgaa ccgcgaggcg cttaccgagt cggcggccga ggccgacgac
    10441 gccaccctga acaagtacct ggaatcgggc cagctgggcg aggcggagtt tcacaccggc
    10501 atccgccagg tcctgctgac ccgcgccctc gtaccgctct ttgtcggttc ggccgccaag
    10561 ggcgaggggc tggaggcctt cctcgatttc gtctgcgagt acctgcccga gtacgaggaa
    10621 cttccgccgg tctccctggc gggcggcgcc gagacgagcc ggcggctggg ctccgagccg
    10681 gtcatgcttc acgttttcaa gacggccagc gatccgttcg tgggccgtct ttcctatttc
    10741 aaggtcatcc gcggcaccct ggcccccaac tccacgctct acaaccagaa ccgctccgag
    10801 aaggagcggg tggggcaggt gctctacgtc cagggcaaga agcaggagcc ggtcgagaag
    10861 gtgcagccgg gcgacctgct ggcggtggcc aagctgaatt acacccacac cggcgatacg
    10921 ctggtcgccg tcggccagaa cgggcacctg ccggccctgg tcttccccca gccggccatt
    10981 tcggtttcgc tggtggccaa ggagaagggc atggaggata agctgggctc ggccttcagc
    11041 cggatcatgg aggaggacca gaccatccag atcgcccgcg acgcggagac gagcgagacg
    11101 attctctccg gcctgggcga cctgcagctg gagatctgga tcagccgcct ggccaaccgg
    11161 ttcggggtcg aggtggtcaa gggcgtgccc aagatcgcct accgggagac ggtgaccggg
    11221 accgccagcg gccagggccg cttcaagcgc cagaccggcg gccacggcca gtacggcgac
    11281 tgctggatca aggtggaacc cctgccgcgc gggagcggct tcgagttcgt cgacgccatc
    11341 gtgggcggcg ccatcccccg ccagtatatc ccctcggtgg agaagggcgt gcgcgaggcg
    11401 atgaccaagg ggccgctggc caactacccg atgaccgacc tgcgggtcac cctttacgac
    11461 ggcaccttcc acccggtcga ctcctccgac atcgccttcc agatcgccgg ctcgctggcg
    11521 ctcaagaagg tcggcctgga ggccaggccg gtcctgctgg agccgatcgt gaacgtcgag
    11581 atcctggtgc ccaaggagaa cgtgggcgac atcatcggcg acatcaacgg ccggcgcggc
    11641 aaggtgctca acatggacag cgtcgacaac gagtaccaga agatttcggc ccagatgccg
    11701 ctggccgaga tgtccaacta cgccaccacc ctgcgctcga tcaccagcgg ccgggcctcc
    11761 taccaggccc agttcagcca ctacgaactc gtcccctacc agatttccga gaagatcatc
    11821 aaggaacgcc aggtgaagaa agaggaagac tgagcatggt tgaagtccgg atcaagggaa
    11881 ttgtggccga aaaggcctcc gggcgcaaca tcctggtcct ggaggacggc gccaagcgcc
    11941 tgctctccgt caccatcggc gtcaccgagg cgcaggcgat catcctgagc ctcaagaaac
    12001 tcggtgtccc ccagccgctg gtttacgacc tgctggtcaa gctggccggc gaactgggcg
    12061 cccggccgct ccggatcgag gtcctggacc gcagcggcga tctcctgatc acccaactgg
    12121 tctaccaggc cggcgaccgg gtccgttttc tggcctgccg gccgggcgac ggggtcgccc
    12181 tggccctgcg ctgccaggtt ccgattttcg tccacgagaa cctgatgaag atggtcaccc
    12241 tgaccgccga taccaggcgg aaacggccca aacctccgcc cgcgcccatg accgaggcgg
    12301 aggtggaccg gatcaagacc gaaatcgaga acctcagcgc cgaggagttc tggcgccggg
    12361 tctccagtcg aaccgagggt gaatcaggag gatcataaca tgagtggaca ttccaagtgg
    12421 gcgagcatca agcacaagaa ggcggcggtg gatgccaagc ggggcaaggc cttcaccaag
    12481 gtcatccggg agataatgat ggccgcgcgg ggcggcgggg ccaaccccga caacaacccg
    12541 cgcctgcggc tggcgatcga caaggcgcgc gacctgaaca tgccggccga caacatcaag
    12601 cgcgccatcc agaaggcgac cggggagggg atggagaacc agctggaaga ggtttcctac
    12661 gagggttacg gcccggccgg ggtggccatc aagctggagg tggtcaccga caacaagcgg
    12721 cggaccgccg ccgagatccg ttccatcttc accaagcaga acggcagcct gggtgaagtc
    12781 ggctgcgttt cctggctctt ttcgcgcaag ggtcagctga ccgtgccgcg ggccggcgtc
    12841 gacgaggaca agctgatttc gctggccctg gacgccggcg ccgaggacgt cgcctccgag
    12901 accgacaact acctggtcta tacccgggcc gaggatatgg acaaggtccg caaggcgctc
    12961 gaggataacc agatcaaggt cggctccgga accctttcga tgcttcccaa gtcgagcgtc
    13021 aaggtggagg gcaaggaggc cgagcagctc ctgaagctgc tggacgccct cgaggaccag
    13081 gaagacgtcc
//
LOCUS       DAOQYN010000025        12916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_5346_length_12916_cov_5.075733, whole genome shotgun sequence.
ACCESSION   DAOQYN010000025 DAOQYN010000000
VERSION     DAOQYN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12916
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5346_length_12916_cov_5.075733"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="PKN80_01975"
     CDS             <1..1580
                     /locus_tag="PKN80_01975"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNQ34812.1"
                     /translation="KPLLGLAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPE
                     DDPRNPAVIADNVGDNVGDIAGMGADLYESYLDSIVAAIALSVAAGLSLSGIALPMIF
                     ATGAAIISILATGLVRTGEKASQPELLQALRRGTYGASILTALLALVLVRIIMPGNFG
                     VFWAIMAGLVGGNLIGYFTEYYTSDSYRPTQEVAEASQTGPATVILEGLGVGMFSTIL
                     PVIVVVIASLAAYYLTGGRANPELGLYGIAIAAVGMLSTLGITLATDAYGPVADNAGG
                     NAEMTHQPPEVRQRTDALDSLGNTTAATGKGFAIGSAALTALALIVSFRDSITNLVPG
                     QLSFSITDPNLIAGILIGAMYPYFFCSMAIKAVGRSAQRIVAEVRRQFKSIAGLMEGK
                     ARPDYARAVDICTQGALREMLVPSIVTIITPVAVGIFLGVGGVMGLLAGATASGFVVA
                     IMMANSGAAWDNAKKYIEAGHLGGKKSDAHKAAVMGDTVGDPFKDTAGPSLNILIKLM
                     SIVAIVFAGLIVKFSLF"
     gene            1581..2753
                     /locus_tag="PKN80_01980"
     CDS             1581..2753
                     /locus_tag="PKN80_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNQ34813.1"
                     /translation="MDYFRESLELHRASRGKIELRSRVPLRDKRDLSLAYTPGVAAAC
                     QEIHRDPARVYDYTSRGNLVAIVSDGSAVLGLGNIGPEAALPVMEGKAILFKEFGGVD
                     AFPIVLASQDTEAIIRTVTMLAPGFGGINLEDISAPRCFRIEAALKEALDIPVFHDDQ
                     HGTAIVVYAALVNALKLAGKRMDNLRVVISGAGAAGIAVARILMDVGVPEIIICDRRG
                     AIYQGRTGDDDNEAKLEMARISNPGKRKGTLAEVLEGADVFIGVSGPGLLTGEAIRRM
                     GKGPIIFALANPVPEIDPEAAHQAGALVVATGRSDFPNQVNNLLAFPGIFKGAFQARA
                     KKITESMKQAAGVAIASLVTAQELTPEYIIPSPLDRRVAPVVAEAVQKACQAAEKA"
     gene            complement(2831..4234)
                     /locus_tag="PKN80_01985"
     CDS             complement(2831..4234)
                     /locus_tag="PKN80_01985"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ34814.1"
                     /translation="MKTILTDDARNTLDQRQKTALFLLLAAAGFLLFWRRPLVLWLFN
                     LLASFFYLTSSFYKFLLVFLGLYRRSEIFVPVEAIDRLGPDELPVYTILLPLYRETEV
                     LDQLISSISRLDYPAERLDVKLLIEANDAPMQAALAARSLPANFELVIIPDSLPKTKP
                     KACNVGLEQARGEFLVIYDAEDIPDSDQLKKSVWAYRNLQDPRVICLQAKLNYYNSRQ
                     NLLTRWFTAEYSMWFDLYLPGMDFLQVPIPLGGTSNHFRTGKLKELGGWDPYNVAEDC
                     ELGIRLFRAGYRTRVLDSTTWEEANSNLGNWLAQRSRWVKGYLQTFLVHHRKPVRLVR
                     ELGWFNYFSFSLSVGGLFVVLLLNPLYWVTTSLWLLRYWGRPELLFPNPYYELVTRAL
                     LAGNFIFILLNLLGCVRRRLYYLVPVTFIAPVYWFLMSLGAWQGFRQLIFSPHYWAKS
                     RHGLTVPLLEAGEAGVE"
     gene            complement(4231..4860)
                     /locus_tag="PKN80_01990"
     CDS             complement(4231..4860)
                     /locus_tag="PKN80_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34815.1"
                     /translation="MLILGVAAVRAELPVRLLFDFESGVQGWAPAAGLPANGELTRPD
                     SGLDGSACLQFGLNLPGEAGLAVKIGDNWQAYQSLLIDLSVSDSAPADLEFLAYLKDK
                     EWLWYQSRPRRLEPGARVTIGLGLSGLSLAWSPERHKRPWNFYSTERINELGLKFLSR
                     QPGRAWVRIDNIRLDPGLFVTFEPGRAASGRTSIPAAVFNLGDNGLTNP"
     gene            complement(5044..5715)
                     /locus_tag="PKN80_01995"
     CDS             complement(5044..5715)
                     /locus_tag="PKN80_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNQ34816.1"
                     /translation="MNGGRRRNGFTLIELLVVMAIISMLAGQMLPALAGAREKGRQAN
                     CINNLKQFSLAIEMYRNDFINEFPCWLSNLYPSYLSSRKVYVCLSDLNDGTAGHGNPT
                     YPETADYSGNTDPDPAIGLRNSQVEACSYFYEFNAAQCDWWESSYGTTGDLNGNGIVS
                     WKEAKRCQMTGVDGVPAITAYFGHVPIVRCFWHYYTHDGPVLNLAVEDYNVYLSDAFW
                     ETTSR"
     gene            complement(5712..6404)
                     /locus_tag="PKN80_02000"
     CDS             complement(5712..6404)
                     /locus_tag="PKN80_02000"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ34817.1"
                     /translation="MAEKDQKQFLKIAGLTLLIVIFIALGFARGCRRGRAPSESLHLE
                     KLKDDKVMVRIEAAFALSRQGTPAALPALEKTLKEDSDPGVRRAAAWAIASIRPENLK
                     PYLTGTDAFLKLVALEKLGELDFRAGRRDFQADLIPRIRDGNLEVARTAVTLVARSGG
                     PKALTALEDAARGSSLDGSVRLLAIRKLGETGDDSVLTTLNEISLKEGGELRQAAQEA
                     VKSIAERKRGGT"
     gene            complement(6445..7107)
                     /locus_tag="PKN80_02005"
     CDS             complement(6445..7107)
                     /locus_tag="PKN80_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNQ34818.1"
                     /translation="MHRRGFTLIELLVVIAIINILASQMLPALSTAREKGRQANCINN
                     LKQFSLAIEMYRMDYSYNFPYWLSNLQPTYLGNSPKILRCLTDQNDGLLGHGFEEWGA
                     SASGNWSGCDIYGQSKAARNDNLGDGPTGNTPRNCSYLYMFNPDPAPDVVVVPEGLAS
                     GTSWRDYSIHMLSASPYRGHTPAVRCLWHKNRALGPVLNIASEDYGTYLSEYYDWTTS
                     YR"
     gene            complement(7152..7865)
                     /locus_tag="PKN80_02010"
     CDS             complement(7152..7865)
                     /locus_tag="PKN80_02010"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2113 family protein"
                     /protein_id="HNQ34819.1"
                     /translation="MLKFPRFIRRIPEERRRWLWIAIGVVLLSSILLLLRTCGRPAVG
                     SGRRSLQDLASPSPERRIEAIHGLVRLRLKSAQPELEKLLANDPEDRVRRAAAYALLT
                     LDPEGFRARLPAGQNDRVTIICLETLARQEREKALPELAELLRSGPVPVRQAALRELG
                     SIGGAEAVEILLRLAEETRLDSQLQVEALVRLGEMAGLDAVERLTRRAEKEPDLQLRR
                     LILDTAARIEKRTRPDQPK"
     gene            complement(7928..8212)
                     /locus_tag="PKN80_02015"
     CDS             complement(7928..8212)
                     /locus_tag="PKN80_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34820.1"
                     /translation="MDYTMGYLLALLLVGVGILAFILALIVLEVSKPKFVVSLILSLL
                     LVIAGGYYYYVVMQMEKGVANANIIHGLFSPAAPTVEAPVVPEVPETPAR"
     gene            complement(8346..8918)
                     /locus_tag="PKN80_02020"
     CDS             complement(8346..8918)
                     /locus_tag="PKN80_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34821.1"
                     /translation="MADIAVSVVSLLALLLSALGVAGFILALILQKFNRPKSVVALVL
                     SLLVLAGGPYFCWRAFRQPVPDVAGSKLFGRDPVRNPGLSRASGIILEISQGEKKLTL
                     ADGETLSLKKGDRITLTGVKVPGVAPSRIRVNVVGFIGNPNREAEDRNVEIDTNGFLR
                     KFALNPEGTRYKVAVLRDKEEIAAFFLDLK"
     gene            complement(8952..9206)
                     /locus_tag="PKN80_02025"
     CDS             complement(8952..9206)
                     /locus_tag="PKN80_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ34822.1"
                     /translation="MGKTGFEVKIDIERCKGCGLCLAFCARGRLVLSETKNRRGCHYV
                     RVRPDGQACNGCLRCALICPDAALEIIRVEAAALAAEPEK"
     gene            complement(9217..11037)
                     /gene="dxs"
                     /locus_tag="PKN80_02030"
     CDS             complement(9217..11037)
                     /gene="dxs"
                     /locus_tag="PKN80_02030"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNQ34823.1"
                     /translation="MILEKLNRTGDIKALPFQELPELARELRQLVIQTVSETGGHLAS
                     NLGVVELTIILHRLLDLDRDAIVWDVGHQCYIHKILTGRREAFRRLRTYRGLSGFPDP
                     NESPADPFRTGHSSTSISTALGLVAGGHPGRVVAVIGDASLGGGVAFEGLNQAAASQA
                     NLCVVLNANDMAISRTTGGVALSLTKLIANHQMEALRQHIRSFLTRFPLLGPDTVGLL
                     HKLEEGIKNLLGPAALFESIGFRYFGPFDGHNLEQLQEAFRKILELSGPRLIHVVTRK
                     GRGYAPAEKDPESFHSTEPFEVETGRPKPGRAPEVSYREVFSRKLLELAADRPELVAV
                     TAAMARGTGLEPFRKEYPERFFDVGIAEQSAVAFAAGLARAGKRPVVAIYSTFFQRAF
                     DQVFQEVCLQGLPVVLVLSNNGLVGEDGPTHHGLFTLSYLRSLPGLRILAPGSYEQLG
                     ELLVEAVSGPGPALLVYPKDRGPERLETLEPGARFRLLAAGSIGAAAREAVERLRADG
                     LPAGFIPIGHLKPLNDDFLTGVVESAAGLVTVEENVSGGFGSAVLEWCGNRGRLLPTL
                     NIKAPDRFVTFGRRRELLAEIGLDAAGIYRQAAGFIRNST"
     gene            11198..12175
                     /locus_tag="PKN80_02035"
     CDS             11198..12175
                     /locus_tag="PKN80_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ34824.1"
                     /translation="MTPRETFIAALERKPITGRVPHFELDFFLTMEAFGKVHPRQRHY
                     YQWDQMTEKERRLHRNEIADIWLAVAERFGHSAIFVRPNPGHPEAEVFRMLDLLREKS
                     GDRYCLLLGGDCTYGIPSGSKMMEFTSRLADEPEKMKAEARDNVNRSLDMAERCARHG
                     GLDGYVLTVDYCFNTGPFLSPGMFSEFVTPYLSDLIKGYRDRGFYTIKHCDGNIMPIL
                     DQLVQARPDALHSIDPQAGVDIAEVKRLAGDRVCLVGNVNCGLLQTGTDQEVIDSARY
                     ALRSGMPGYGYIFATSNVVYTGMRLDRYELMLSVWRTEGNYPEPEARNA"
     gene            12168..>12916
                     /locus_tag="PKN80_02040"
     CDS             12168..>12916
                     /locus_tag="PKN80_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34825.1"
                     /translation="MLEEYRAAFEETAAWWHRENTRPCLVTGRLKRGQLPVKSDANRF
                     WPGVDSNPDLDGLLAEFAAGVEKTEGEYAWLPKIPFRFGGRGTAMTIAYYLGGRVLFG
                     PSTVWVEPVIRNWSDFSVRFDPENPWWQHTLRYLEKAVELSRGRFLVCLPDFGDALTT
                     LALLRGTENLLLDLMDNRSAVIRARDQFLQLWPEFHRACWDIYRRHLPGDNSWLLWAP
                     GRTYACQSDFSAMISPELFRELVVPEIEFLGG"
BASE COUNT         2417 a         4177 c         4046 g         2276 t
ORIGIN      
        1 tcaagccgct gctgggcctg gccttcggct cctcgctgat ctcgatcttc gcgcggctgg
       61 gcggcggcat cttcaccaag ggcgccgacg tgggcgccga cctggtgggc aaggttgagg
      121 ccggcatccc ggaggatgac ccgcgcaacc cggccgtcat cgccgacaac gtgggcgaca
      181 acgtcggcga catcgccggc atgggcgccg acctttacga atcatacctt gactccatcg
      241 ttgccgcgat cgccctctcg gtggccgccg gcctctcgct ctccgggatc gccctgccga
      301 tgatcttcgc cactggcgcg gccatcatct cgatcctcgc caccggcctg gtgcgcaccg
      361 gcgagaaggc cagccagccg gagctgctcc aggccctgcg ccgcggcact tacggcgcct
      421 ccatcctgac cgccctgctg gccctggtcc tggtccggat catcatgccg ggtaatttcg
      481 gcgtcttctg ggccatcatg gccgggctgg tgggcggcaa cctgatcggc tacttcaccg
      541 agtactacac ctcggacagc taccgtccca cccaggaagt ggccgaggcc tcccagaccg
      601 gaccggccac cgtcatcctg gaggggctgg gcgtcgggat gttctcgacc atcctgccgg
      661 taatcgtggt ggtgatcgcc agcctggcgg cctactacct gaccggcggc cgcgccaacc
      721 cggaactcgg cctttacggt atcgccatcg ccgcggtcgg catgctttcc accctgggca
      781 tcaccctggc caccgatgcc tacggaccgg tggccgacaa cgccggcggc aacgccgaga
      841 tgacccacca gccgcccgag gtccggcagc ggaccgacgc cctggactcc ctgggcaaca
      901 ccaccgcggc caccggcaag ggcttcgcca tcggctcggc cgccctgacc gccctggccc
      961 tgatcgtctc cttccgggat tcgatcacca acctggtgcc cggtcagctc tccttctcca
     1021 tcaccgaccc gaatctgatc gccggcatcc tgatcggggc catgtacccc tacttcttct
     1081 gttccatggc catcaaggcg gtcggccgca gcgcccagcg catcgtggcc gaggtgcgcc
     1141 gccagttcaa gagcatcgcc ggtctcatgg aaggcaaggc ccggccggac tacgcccggg
     1201 cggttgacat ctgcacccag ggcgccctcc gggagatgct ggtaccctcg atcgtcacca
     1261 tcatcacccc ggtggccgtc ggcatcttcc tgggcgtggg cggcgtgatg ggcctgctgg
     1321 ctggcgccac cgccagcggc ttcgtggtcg ccatcatgat ggccaactcc ggcgcggcct
     1381 gggacaatgc caagaagtat atcgaggctg gacacctggg cggcaagaag tccgacgccc
     1441 acaaggcggc ggtcatgggc gacacggtcg gcgatccctt caaggacacc gccggcccct
     1501 cgctcaacat cctgatcaag ctgatgtcga tcgtggccat cgtcttcgcc ggcctgatcg
     1561 taaagttcag cctcttctaa atggattatt tccgggaatc actggaactg caccgggcca
     1621 gccgggggaa gatcgagctg cgcagccggg tccccttgcg cgataagcgg gacctgtcgc
     1681 tggcctatac ccccggcgtg gccgccgcct gccaggagat tcaccgggac ccggcccggg
     1741 tctacgatta cacctcccgg ggaaacctgg tggcgatcgt cagcgacggc agcgccgtgc
     1801 tcggcctggg caacatcggc ccggaggcgg ccctgccggt catggaaggc aaggccatcc
     1861 tcttcaagga attcggaggc gtggacgcct tcccgatcgt gctggccagc caggataccg
     1921 aggcgatcat ccggacggtg accatgctgg cgccgggctt cgggggcatc aacctcgagg
     1981 acatctcggc gccgcgctgc ttcaggatcg aggcggcgct gaaggaggcg ctggacatcc
     2041 cggtcttcca cgacgaccag cacggcacgg ccatcgttgt ttacgcggcc ctggtcaacg
     2101 ccctcaagct tgccggcaag cggatggata acctgcgggt ggtgatttcc ggggccggcg
     2161 cggccgggat cgccgtggcc aggatcctga tggacgtggg agtgccggag atcatcatct
     2221 gcgaccgccg gggcgccatt taccaagggc ggaccggcga cgatgacaac gaggccaagc
     2281 tggaaatggc ccggatcagc aacccgggga aacggaaggg aacgctggcg gaggtactgg
     2341 aaggggccga tgtctttatc ggcgtctcgg gaccgggcct cctgaccggc gaggcgatcc
     2401 ggcggatggg aaaagggcca atcatcttcg cgctggccaa cccggtgccc gagatcgacc
     2461 ccgaagcggc ccaccaggcg ggcgccctgg tggtggccac cggacggagc gacttcccca
     2521 accaggtgaa caacctgctg gccttcccgg gcatcttcaa gggagccttc caggcccggg
     2581 cgaaaaaaat caccgaaagc atgaagcagg ccgccggggt cgccatcgcc tccctggtaa
     2641 cggcccagga actcaccccg gagtatatca ttccttcacc gctggaccgc cgggtggccc
     2701 cggtggtggc cgaggccgtt cagaaggcct gccaggcggc tgaaaaagcc tgaaaccgcc
     2761 ttcccctcaa acaattacgt ccgcttttcc ctcgctgccc caaacaacaa ccggcctttt
     2821 atccgcccga ctattccacc ccggcttccc cggcctccag cagggggacg gtcaatccgt
     2881 ggcggctctt ggcccagtaa tggggggaga agatcagctg ccggaaaccc tgccaggcgc
     2941 ccaggctcat caggaaccag taaacgggcg cgatgaaggt aaccgggacc aggtaataaa
     3001 gacggcggcg gacgcacccc agcaggttca gcaggatgaa gatgaagttc ccggccagca
     3061 gggcccgggt aaccagctcg tagtaaggat tcgggaagag cagctccggc cgcccccagt
     3121 aacgcaacag ccagaggctg gtggtcaccc agtagagggg attcaagagc aggaccacga
     3181 agagcccgcc gacgctcagg ctgaaactga aataattgaa ccagccgagt tcccggacca
     3241 gccggaccgg ttttcgatga tggaccagga aggtctgcag gtaacccttc acccaacggg
     3301 aacgctgggc cagccagttg cccaggttgc tgttggcctc ctcccaggtg gtgctgtcca
     3361 gcacccgggt acggtagccg gcccggaaga gccggatgcc cagctcgcaa tcctcggcca
     3421 cgttataggg gtcccagccg cccagctcct tcagtttgcc ggtgcggaaa tggttggagg
     3481 tgccgcccag cggaatcgga acctggagaa aatccatgcc gggcaggtag agatcgaacc
     3541 acattgaata ttcggccgtg aaccaccggg tcagcaggtt ctgccggctg ttgtagtaat
     3601 tcagcttggc ttgcaggcag atcaccctgg ggtcctggag gttccggtag gcccagacgc
     3661 tcttcttcag ctggtcggaa tccgggatgt cctcggcatc gtagatgacc aggaattcgc
     3721 cccgggcctg ttccaggccg acgttgcagg ccttcggctt ggttttgggc aacgagtccg
     3781 ggatgattac cagttcgaaa ttggcgggca gggagcgggc ggccagggcc gcctgcatcg
     3841 gggcgtcatt ggcctcgatc agcagcttga cgtcgagccg ttccgcggga taatccagcc
     3901 ggctgatcga ggaaataagc tggtccagca cctcggtctc ccggtagagg ggcaggagta
     3961 tcgtgtaaac cggcagttca tcaggaccga gccggtcgat cgcttcgacc ggaacgaata
     4021 tctcgctgcg gcggtacaat ccgaggaaaa ccagcaggaa cttgtagaag gaactggtca
     4081 ggtagaagaa actggccagg agattgaaaa gccagagtac aagcggacgg cgccagaaga
     4141 gcaaaaaccc ggcggcggcc agcagtagaa acagggccgt tttttgccgc tggtccaggg
     4201 tgttgcgcgc atcgtcggtc aaaatggtct tcatggattc gtcaggccgt tatcgccgag
     4261 gttaaaaacc gccgccggga ttgatgtccg gccggaagcg gcccggccgg gttcaaaggt
     4321 gacaaacaag cccgggtcga gacggatatt gtcgatccgg acccaggccc ggccgggctg
     4381 ccgggagagg aatttcagac ccagctcgtt aatccgttcg gtgctgtaga agttccaggg
     4441 tcgtttgtgc ctttccgggc tccaggccag gctgaggccg gaaagtccca ggccgatcgt
     4501 aacccgggcg cccggctcga gccggcgcgg ccggctctgg taccagagcc attccttgtc
     4561 cttgagatag gccaggaact ccaggtcggc gggagcgctg tcggaaacgg acagatcgat
     4621 gagaagcgac tggtatgcct gccagttgtc accgattttc accgccaggc cggcctcacc
     4681 cggcaggttc agcccgaact gcaggcaggc cgagccatca aggccggaat cgggccgggt
     4741 cagttccccg ttggccggca gtccggccgc cggggcccag ccctggaccc cggattcaaa
     4801 atcaaagagg aggcggaccg gcagttcggc ccggaccgcc gccaccccca aaatcagcat
     4861 cccggcaagg ataaccattg ccgggaatag aagcctgcct ccccatccag aacgattcat
     4921 caataaaacg gcgcgccgct tcctttctat ccaccccttt caaaatcggg agcctttccg
     4981 gagatccggt ttaccgcccc gaaaccggct gccggaccgg aaccggtccg gtatcccggc
     5041 ggctcaacgc gaggtggtct cccagaaggc atcgctcagg tagacgttgt aatcctcgac
     5101 ggccaggttg agtaccggcc cgtcgtgcgt gtagtaatgc cagaagcacc tgacgattgg
     5161 aacatggccg aagtaggcgg tgatcgcggg cacaccgtcg acgccggtca tctggcagcg
     5221 tttggcctct ttccaggaga cgatcccatt gccgttaagg tcaccggtgg tgccgtaact
     5281 ggactcccac cagtcgcatt gggcggcgtt gaactcgtaa aagtagctgc aggcctcgac
     5341 ctggctgttg cgcagcccga tggccggatc cggatcggta ttgccgctgt aatcggccgt
     5401 ctccgggtag gtggggttgc cgtgcccggc ggtcccgtcg ttcaggtcgg aaaggcagac
     5461 atacaccttc cggctggaca ggtagctcgg gtaaaggttg gagagccagc agggaaactc
     5521 gttgatgaaa tcattgcggt acatctcgat ggccagcgag aattgcttga gattgttgat
     5581 gcagttggcc tggcgcccct tctcgcgggc cccggccagg gccggcagca tctggccggc
     5641 cagcatggag atgatggcca tgaccaccag cagttcgatc agggtaaagc cattccttcg
     5701 ccttccaccg ttcatgttcc acctcgtttg cgttccgcga ttgacttaac cgcctcctgg
     5761 gccgcctggc gcagttcccc gccctctttc aagctgatct cgttcagggt ggtcagcact
     5821 gaatcatcgc cggtttcacc cagtttccgg atggccagga gccggaccga cccgtccagg
     5881 ctgctgcccc gggcggcatc ctccagggcg gtcagggcct tgggaccgcc ggaccgggcc
     5941 accagggtga ccgccgtccg ggccacctcc aggttcccgt cccggatccg cggaatgaga
     6001 tcggcctgga aatcacggcg gccggcccgg aagtcgagtt cccccagttt ctcgagggcg
     6061 accagtttca ggaaggcgtc ggtcccggtc aggtagggct tcaaattttc cggcctgatg
     6121 gaggcgattg cccaggccgc cgcccggcgc accccgggat cgctgtcctc cttcaacgtc
     6181 ttctccagtg ccggcagcgc cgccggcgtg ccctgccggc tgagggcgaa ggcggcctcg
     6241 atccggacca tgaccttgtc atccttgagc ttttccaggt ggagcgattc gctgggggcc
     6301 cggccccggc ggcagccgcg ggcaaatccc agcgctatga agatgacaat caggagcgtc
     6361 aggccggcga tcttcagaaa ctgcttctgg tctttctccg ccatcttttg ttttcccgtt
     6421 tttctcgcct tgaaggcgtc cggatcagcg ataggaggtc gtccagtcgt aatactcgga
     6481 gagataggtg ccgtaatcct cgctggctat gttcaggacc ggccccagcg cccggttctt
     6541 gtgccacagg cagcggactg ccggcgtgtg tccccggtag ggagaagcgc tcagcatgtg
     6601 gatggagtaa tcccgccagc tggtgccgct ggccagtccc tccggaacca ccaccacgtc
     6661 cggggccggg tccgggttga acatgtaaag gtaggaacag ttgcgggggg tgttgccggt
     6721 cgggccgtcg ccgagattgt cattgcgggc ggccttggac tggccgtaaa tatcacaacc
     6781 gctccagttg ccggaagcgc tcgcgcccca ctcctcgaaa ccgtgtccca ggagaccgtc
     6841 attctggtcg gtcaggcaac gcagaatctt gggggagttc ccgagatagg tgggctgcag
     6901 gttgctcagc cagtagggaa aattgtagct gtaatccatc cggtacatct cgatggccag
     6961 ggagaattgc ttgaggttgt tgatgcagtt ggcctgccgc cccttttccc gcgcggtgga
     7021 aagcgccggc agcatctggc tggccagaat gttaatgatg gcaataacca ccagcaattc
     7081 gatcagcgta aatccccgtc ggtgcattaa tcacctccga gattatttgt taacgaaggc
     7141 agggtttttg tttatttcgg ctggtccgga cgcgtccgct tctcgatccg ggcggcggtg
     7201 tccaggatca gccggcgcag ctgaaggtcc ggctcctttt cggcccgcct ggtcaggcgc
     7261 tcgaccgcat caagtccggc catttccccc aggcggacca gcgcctccac ctgcagttga
     7321 ctgtccagcc gggtctcctc cgccaggcgc aaaaggatct cgaccgcttc ggctcccccg
     7381 atcgaaccca gctccctgag ggcggcctgg cggaccggaa ccgggccgct tctgagcagt
     7441 tccgccagtt ccggcagggc cttttccctt tcctgccggg ccagggtttc caggcagatg
     7501 atggtcaccc ggtcgttctg cccggccggc agccgcgccc gaaaaccctc cggatccagg
     7561 gtcagcaggg cgtaggcggc ggcccggcgc acccggtctt cgggatcgtt ggccaggagc
     7621 ttctcgagtt cgggctgggc ggatttcaag cgcaggcgga ccagcccgtg aatggcttca
     7681 atccgccttt ccgggctggg ggaggcgagg tcctgaaggc tccggcggcc ggagccgacc
     7741 gccgggcggc cgcaggtacg cagcaggagc agtatcgagg aaagcaaaac gacgccgatg
     7801 gcaatccaga gccaccgacg ccgttcttcc gggattcttc ttatgaacct gggaaattta
     7861 agcatgtcaa aaccccggtt acccggtgcc ttgaggcacc gggtgacaaa cagttgaaaa
     7921 ccggggctta ccgcgccgga gtttccggaa cctccggaac aaccggcgcc tctaccgtcg
     7981 gagcggccgg ggagaagagg ccatggatta tgttggcgtt ggcgacgccc ttctccatct
     8041 gcattacaac gtaatagtaa tagccgcccg cgatcaccag gagaagagaa aggataagag
     8101 agaccacgaa cttcggcttg ctgacctcca ggacgatcag ggccaggatg aaggccagaa
     8161 ttcccacgcc gaccaacagc aacgcgagca aataccccat cgtataatcc ataaccaccc
     8221 tcctgatttc tcagcgttta aacaaaaaaa agagctcctc gacttttaga gccggaccaa
     8281 ttccattata ggagtatttt atcacccccg ttaccggctg tcaacctgac ccggcaccgt
     8341 ccggattact tcaaatcgag aaagaaggcg gcgatctcct ccttgtcccg cagaacggcc
     8401 accttgtagc gggttccctc cgggttcagg gcgaatttcc tcaggaaacc gttcgtgtcg
     8461 atctccacgt tgcggtcttc ggcctcccgg ttcgggttcc cgatgaagcc gaccacgttg
     8521 acccggatcc gggacggcgc cacccccggg actttcacgc cggtcagggt gatccggtct
     8581 cccttcttca gagagagggt ctcgccgtcg gccagggtga gctttttttc tccctggctg
     8641 atttcaagga tgatcccgga ggcccgggag agacccggat tccgaaccgg gtcccggccg
     8701 aagagtttcg aaccggcgac atccgggacc ggctgccgga aggcccgcca gcagaagtag
     8761 gggccgccgg ccagcacaag cagggagagt accagcgcca ccacggactt cggccggtta
     8821 aacttctgaa gaataagggc caggatgaac ccggccacgc caagggccga aagaagaagt
     8881 gccagcaggg aaaccacgct aaccgcgata tccgccatat ttcctccttg aacctggttc
     8941 gatttattct atcacttctc cggctcggca gccagggcgg ccgcctcgac gcggataatc
     9001 tccagggcgg cgtccgggca gatcagggcg cagcggagac agccgttgca ggcctggccg
     9061 tccggccgca cccggacata atggcagccc cgccggttct tggtctccga aagaacgagg
     9121 cggccgcggg cacagaaggc caggcaaaga ccgcagccct tgcagcgctc gatatcgatc
     9181 ttcacctcga agccggtctt gcccatggtc ctcctgtcag gttgaattcc ggatgaaacc
     9241 ggccgcctgc cggtaaatac ccgccgcgtc cagcccgatc tccgccagca attcccggcg
     9301 ccggccgaag gttacgaaac ggtcgggcgc cttgatattg agggttggaa ggagccggcc
     9361 ccggttaccg caccattcca ggaccgcgct gccaaagccg ccgctgacat tctcctccac
     9421 cgtaaccagg ccggcggccg actcgaccac cccggtcaaa aaatcgtcat tcaacggctt
     9481 gagatgtccg atcgggataa acccggccgg caggccgtcg gcccgcagac gttcaaccgc
     9541 ctcccgggcc gccgcaccga tgctgccggc cgccagcaga cgaaaacggg cccccggctc
     9601 cagcgtttcc agccgttccg ggccccggtc cttgggatag accagcaggg ccggtccggg
     9661 ccccgaaacg gcctccacga gcagctctcc gagctgttcg taggaaccgg gagccaggat
     9721 tcgcaaaccc ggcagggaac gcaggtagga gagggtgaag agcccgtgat gggtgggtcc
     9781 gtcctctccg accaggccgt tgttggaaag gaccaggacc accggcaggc cctggaggca
     9841 gacctcctgg aaaacctggt cgaaggcgcg ctgaaagaag gtggaataaa tggcgaccac
     9901 cggtcttttt ccggcccggg ccaggccggc cgcgaaggcc accgccgact gctcggcgat
     9961 gccgacgtcg aagaaacgtt ccgggtactc cttccggaac ggctccaggc cggtaccccg
    10021 ggccatggcg gcggttaccg ccaccagttc cgggcggtcc gcggccagtt caagcagctt
    10081 ccttgaaaaa acttcccggt aggagacctc cggggcccga ccgggtttcg gccggcccgt
    10141 ctcgacctcg aacggctcgg tggaatggaa actctccggg tctttttcgg ccggagcgta
    10201 accgcgccct ttccgggtga ccacgtggat taaacggggg ccggacagct ccaggatttt
    10261 tcggaaggcc tcctgcagct gctccaggtt gtgcccgtca aacgggccga aatagcggaa
    10321 cccgatgctt tcgaaaaggg cggccggccc cagcaggttc ttgatcccct cctcgagctt
    10381 gtgcagcagg ccgaccgtgt ccggcccgag cagcggaaaa cgggtcagga aggaccggat
    10441 gtgctggcgg agcgcctcca tctggtggtt ggcgatcagc ttggtcaggg aaagggcgac
    10501 gccgccggtg gtgcgtgaaa tggccatgtc attggcgttc aacacgacgc agaggttggc
    10561 ctgggaggcc gccgcctggt tgagcccctc gaaggcgacg ccgccgccca gggaagcatc
    10621 gccgatcacc gccaccaccc ggcccgggtg gccgcccgca accaggccca gggcggtcga
    10681 gatcgaagtg ctggaatggc cggtccggaa cggatcggcc gggctttcgt tcgggtcggg
    10741 gaaaccggag aggccccggt aggtccgaag ccggcggaag gcctcccgcc ggccggtcag
    10801 tattttatga atataacact ggtgcccgac atcccagacg atggcgtccc ggtcaaggtc
    10861 aagcaggcgg tgcaggatga tggtcaactc caccacgccg agattggagg ccaggtggcc
    10921 gcccgtctcc gaaacggtct ggatgaccag ctgccggagt tcgcgggcca gttccggcag
    10981 ctcctgaaaa ggcagcgcct tgatatcgcc ggtccgattt aacttttcca gtatcatttt
    11041 tcgcggtcgt gggccaggcc cgtacgttca aatcttaaaa attattattt tatctctttt
    11101 caaggccgtc tgtctaatga aaaatgtgat aaaataaggt ggcataatca tcagccgttc
    11161 atctcggacg gccttcactt tcaacccgga gaacccgatg accccgaggg aaaccttcat
    11221 agccgccctg gaacggaaac ctatcaccgg ccgcgttccc cacttcgaac tcgacttctt
    11281 ccttaccatg gaagccttcg gcaaggtaca tccgcgccag cgccattact accagtggga
    11341 ccagatgacc gaaaaagagc gccggctgca ccggaacgag atcgccgata tctggctggc
    11401 ggtggccgaa cgtttcggcc actcggccat cttcgtgcgt cccaaccccg gccaccccga
    11461 agcggaggtc ttccgcatgc tggacctgct gcgggagaaa tcgggcgacc gctactgtct
    11521 cctgctgggc ggcgactgca cctacgggat cccgtccgga agcaagatga tggagttcac
    11581 cagccggctg gccgacgaac cggaaaagat gaaggcggag gcccgcgata atgtaaaccg
    11641 atcgctcgac atggccgaaa gatgcgcccg gcacggcggg ctggatggat acgtgctcac
    11701 ggtcgattac tgtttcaaca ccggcccctt cctcagtccc ggcatgttct cggaattcgt
    11761 taccccctac ctgtccgatc tcatcaaggg gtaccgcgac cggggctttt acacgatcaa
    11821 gcattgcgac ggcaacatta tgcccatcct ggaccagctg gtccaggccc gtccggacgc
    11881 gctccactcc atcgatcccc aggccggtgt cgatatcgcc gaggtgaaac ggctggccgg
    11941 cgaccgggtc tgcctggtcg ggaatgtcaa ctgcggcctg ctgcagaccg gcaccgacca
    12001 ggaggtgatt gactcggccc gctacgccct ccgcagcggc atgcccgggt acgggtacat
    12061 cttcgccacc agcaatgtcg tctacaccgg gatgcggctc gaccgttacg aattgatgct
    12121 gtcggtctgg agaaccgagg gaaactaccc cgaaccggag gcccgaaatg cttgaggaat
    12181 accgtgccgc ttttgaagag accgccgcct ggtggcaccg ggagaacacc cgtccctgcc
    12241 tggtgaccgg ccggctgaaa cgggggcagc tgccggtcaa atccgatgcc aaccgcttct
    12301 ggccgggggt cgattcgaac cccgacctgg acggactgct ggccgaattc gccgccgggg
    12361 tcgagaagac cgagggggaa tacgcctggc tgcccaaaat tcccttccgt ttcggcggcc
    12421 ggggcaccgc catgactatc gcctattacc tgggtggccg ggtgctcttc ggcccttcca
    12481 ccgtctgggt ggaaccggtt atccggaact ggtcggactt ctccgttcgc ttcgacccgg
    12541 aaaacccctg gtggcagcac acgctccgct acctggaaaa agcggtcgag ctcagccggg
    12601 gccgtttcct ggtctgcctt ccggatttcg gcgacgcgct gactacgctg gccctgctgc
    12661 gcggcaccga gaacctcctg ctggacctga tggataatcg ttcggcggta atccgggccc
    12721 gggaccagtt cctgcagctc tggccggaat tccaccgggc ctgctgggac atctaccgcc
    12781 ggcacctgcc cggcgacaac agctggctgc tctgggcgcc gggccggacc tatgcctgcc
    12841 agtcggattt cagcgccatg atctccccgg aactcttccg ggagctggtg gtcccggaaa
    12901 tcgaattcct gggcgg
//
LOCUS       DAOQYN010000026        12427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_5561_length_12427_cov_4.199725, whole genome shotgun sequence.
ACCESSION   DAOQYN010000026 DAOQYN010000000
VERSION     DAOQYN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12427
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5561_length_12427_cov_4.199725"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2240)
                     /gene="gspD"
                     /locus_tag="PKN80_02045"
     CDS             complement(<1..2240)
                     /gene="gspD"
                     /locus_tag="PKN80_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNQ34826.1"
                     /translation="MRNSRLNRALAITWLSLLLLPAAGRTLNLHAQNANGLPDDNAPP
                     VYTPPPSVLTQGDDTTQPPVNPQPAPDQATGVVITNDAAGPATIEAVIIDGGANPAEI
                     PGPGSGGLIMPPTPLPNEPAPARLPSEVPPPVSLPPASAPSAETAVRETTPASPLVPE
                     TIPPTGAAQGIVLNFQNASLDTILAYLSETAGFIVVRDVAVEGHLTIMSRQPITREEA
                     ITVLNTALYQKGYAAIVTGRTLRIIPLADAKKRNLPVRIGNDPSKIEPSDSVVTQIIP
                     VRYVDAVKLRQDLAPLLPASAELIANASSNSLIMTDIEANIRRIMEIVNAVDTAVSSV
                     AEIRVFQLRYANATNTARLINELFRSSQASRQSGQAGAVSGATRFFTARGGQQPGQVT
                     VTTQPAASDGSRPAAQTTATADERTNTIVVSGTSETLKVIETLVKDLDSSPEAEEAVF
                     IYKLKNGTANNVATTLNNLFGTSTASRNQRTNIRMSNPAAAAALDAAGGSSLEGQVYV
                     VANSDTNSLMVMTASKNFDRVKEIIDDLDRPLPQVLIKVLIAEVTYDRSLDLGTEFSI
                     LNLGADAKGSVFTKFGVDQGAGMVVSLVEKDVTAALTALQKVGKLDVLSRPYILGSEN
                     QRAQITVGQRVPFITNTRTTETGQTINTIQYQDVGIILNVTPKINPEGLVILDVSPEI
                     SALTGTTVPISETVNAPVFAKRSATSRVAIQDGQTIVIGGLMEDRKTEEVKKVPLLGD
                     IPLLGLL"
     gene            complement(2249..2836)
                     /locus_tag="PKN80_02050"
     CDS             complement(2249..2836)
                     /locus_tag="PKN80_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34827.1"
                     /translation="MRRLLPVFLWIILSIGAGGRMAGAAEPAPTTTGFPYERYRVLAE
                     RNIYLKNRQPQPGRITSILRETPVVPIVRRMVVLTGIARSGSEFVAFFEDRASGMTTR
                     VRGESSLLGGRIAEINIDYVEYEKDGLVNRIRLGETVPETLARESAPPAPAVKNALPT
                     PASSAPSAYPVAAPIDEARILEQMRQRRRLEVGAE"
     gene            complement(2833..3411)
                     /locus_tag="PKN80_02055"
     CDS             complement(2833..3411)
                     /locus_tag="PKN80_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34828.1"
                     /translation="MILSKRERCIAVITLVAVFFLASDRFLLTPYLERRGRLLSEKHR
                     LAEELESARSLFGRRDEPPTPLEKMLESDAGTVESLTLRSLRNWAEESGLALSMLKPE
                     RSTRIGQMEELSFQAIGAGSMRAIRDFLWKIESARLPIKISELQLSGRKENSDDLMLQ
                     VRVAALCLAPADKQPSKKARSKNNAALEGDAE"
     gene            complement(3408..4889)
                     /locus_tag="PKN80_02060"
     CDS             complement(3408..4889)
                     /locus_tag="PKN80_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34829.1"
                     /translation="MPFIKFGKSRRLGLTLGERSLVAAEIQVTGRTSRLIRVVRWPFP
                     DGLTIGAPLGKALRQFLRTQHIAADTAIAGIPAGWLLTKEKTVPPADPSTLANLLKLE
                     AESDFAFPPADLILEYLEATPAADASRPILLIAALRKRIEALQQMAHAAGLKLAAATP
                     SSLALADTAGSRTDNLVLYLGPEEVELVWLSGGAPLIFRRLGKIPAPAADKLNEETAA
                     WAGEIGTAIQKLLFLMPRREGFVEASTISIRNDSGLPETALQALQRALPLPVKTTKGL
                     EVPEPAMANGSRPAAGEAAFRLALAGERPQALPLDFLHPRLTRFGDDQKPLRRRLLIW
                     SASAALVLLLTAAGGWLETLGHQREINALRQKLEEVRPELARTRKQEDRLRSLRAWQN
                     QEPLFLECLREVTLAFPAEGGIWTSNLAIRADLQGLISGKAARDTAVFDLLEGLKRSP
                     AFSQVNVVYIRETGGGNPEVAYAIRFAFLKPSARKSTATEKTR"
     gene            complement(4889..6088)
                     /locus_tag="PKN80_02065"
     CDS             complement(4889..6088)
                     /locus_tag="PKN80_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNQ34830.1"
                     /translation="MIRQLRLAGRAAPRPGRKKRPGSRGTILIATMWVVLVLAGLVLV
                     FAHRMRVEVLASANQLAAAQAEWVARAGLAFMIAQVNGTDGTWSNDEALSCEAVPVGN
                     GYFWLLHPDPEDDWSYRFGITDESSRLNLNYATEEMLLMFNGLTEELAAALIDWRNPD
                     AEAPPIEGGAKSEYYLLRSPAYNCKSGPLETIEEILMIKGASPEILFGEDLNRNGVLD
                     PNEDDGDENPPADNRDGQLDRGIYPFITVYSEAAAATGGRNRPTSPINVNTAPREVLQ
                     CLPGLDQADVDALLTARAAETTNLENPDWVNQALPAAKAAAIRNWITVQSYRFSADIV
                     AVSGDGRAFKRFRAVVDARTRPPRVLAWQELTGLGWPLDPQVLNDLRSGKEPPVINAI
                     SDAGGVS"
     gene            complement(6085..>6388)
                     /locus_tag="PKN80_02070"
     CDS             complement(6085..>6388)
                     /locus_tag="PKN80_02070"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HNQ34831.1"
                     /translation="APEPPAAETQVICRNVNEFNLRYFDGSEWFDNWDSTGQTDNLPR
                     AVEISLELPLASKEGEPEVYRFRQTVLLPCCNPAAAETVSAPAGSSTQRTGGTGGG"
     assembly_gap    6389..6398
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6399..6874)
                     /locus_tag="PKN80_02075"
     CDS             complement(<6399..6874)
                     /locus_tag="PKN80_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ34832.1"
                     /translation="MNKPSANPCRNKGFTLLEMLLAMTMTALLASSLYASLRIGFRAR
                     EQSEKSLEPFRAAANAFDIMRRDLQSTPPPRGILAGPFLGLDGGEALSESGYDSISFF
                     TQPMGRLNAAPGIIGLEYRLEIPGGESEPALTRLVTTNLLAPETPATRFSQKSCAV"
     gene            complement(6867..7262)
                     /locus_tag="PKN80_02080"
     CDS             complement(6867..7262)
                     /locus_tag="PKN80_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34833.1"
                     /translation="MRIPDLAKKRDAFTLVEVLAALTFTMILLPVAMKGVSLATGASA
                     DARRRIEATLLARTLMAELESEGVNQFSEASGDFAPEAPGYQWSARVAAGPESNLNEL
                     QVEVTWQSRGSERSVTLATLIYEEGVSNE"
     gene            complement(7267..7872)
                     /locus_tag="PKN80_02085"
     CDS             complement(7267..7872)
                     /locus_tag="PKN80_02085"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNQ34834.1"
                     /translation="MTISTTGRNGKTRIPVDLGRPPAGFTLLEMILVMVVITIVLSMA
                     APSLSGFARSRPIFDASVKFVSLSDYARSQAVAEGRVYRLNLDTETGRFWLTAQEGGA
                     FKELGQGMGRVFRLPDETRADWIEHPSRTAEASPPEFLSMGSAESLVPRDSGDNEYIE
                     FQPDGSAETAVIRLTDRKNNRLDITSSSPTDRFRVVTSTGT"
     gene            complement(7841..8305)
                     /gene="gspG"
                     /locus_tag="PKN80_02090"
     CDS             complement(7841..8305)
                     /gene="gspG"
                     /locus_tag="PKN80_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNQ34835.1"
                     /translation="MPSKPCKRVKPDNYSIGRSGNRGFTLIELLLVLVILTILAAIVV
                     PRYAKRSEQARITAASTDIASLEAALDLFEVDAGRYPSTEEGLEALVEVPAQLENWKG
                     PYIKRGVPKDPWGNPYVYTSPGDNNSNSYDLYSYGPDGKDGGDDDIDNWSER"
     gene            complement(8345..9574)
                     /locus_tag="PKN80_02095"
     CDS             complement(8345..9574)
                     /locus_tag="PKN80_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ34836.1"
                     /translation="MPVFSYQALNVSGEKVSGTLTAGSRSELLEAIGRQGLRPVSVKE
                     IGAEEAPAGVGIRRRLFSRTAGSQTGVEAFTRQLANLLAAGVPLSRSLDIVTRETSAA
                     DVRQQWNTIRNDIKGGLSFATALSKWPRSFPPVYVAIVRAGETGGFLNLALEQIADFR
                     AREHDLKGRVKAALVYPVLLAGLAGVVLIFLMTYFIPRFSAIFSEFGGSLPWLTRLIM
                     NLSRWLVGHGLILAAAAVAGIISLRRALATETGQRVLERTVLKLPGIGTVVARFALVR
                     FSRMLGTLLGAGVPLVLALNVAREAIGNRTLSDAVTRAVENVKSGSSLAMGLASSERL
                     FPPSVVEMVAVAEESGNLNKELIRLATSYENDLDRQLRMLVSVIEPVLLFLTAGIVGT
                     VVIGMLLPVFTLQDLIR"
     gene            complement(9600..11354)
                     /locus_tag="PKN80_02100"
     CDS             complement(9600..11354)
                     /locus_tag="PKN80_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNQ34837.1"
                     /translation="MIDIVGKLIENKLLNRETAERVRSLSKSGVPLDEALLTGSGLAE
                     ETLLKILAAEFNVSYRDIEESLPGREFLAVFPARILIDRQLLPLSEEAGTVMVATSRL
                     FDTAGIDELRLATGREFKPVLTTPSQIRQGLKICLGIGADTVQSMISDSEEMPITVVT
                     DETESGVDLGAAAEDASIIRFVNQILKEAIELRATDVHFEPFENELRVRYRIDGVLKD
                     AAVPPEIRRFQAAIVSRLKIVSSLDIAEKRLPQDGRIKIFVSGREIDVRVSVIPMLHG
                     EAIVLRLLDRNAALLGLAQLGMSERDRNFFGRMLDLPHGIILVTGPTGSGKTTTLYAG
                     LSTINDIQRKIVTIEDPIEYHLRGINQIQVSNKAGLTFASGLRAILRHDPDVILVGEI
                     RDYETAEIAVQASLTGHLVFSTLHTNDAPGAPTRLVDMGVEPYLVASSLEAVLAQRLV
                     RLICPHCKEEIADADREVLRREYGEEIPPVLFRGRGCRECQGTGYLGRSGVFEFMHVT
                     DDIRALILKRASSREIRAAATRQGMRSLREDGWRLISEGFTTPEEVIRVTKDERVNGT
                     NSYGTAPGELNESGPVLE"
     gene            complement(11392..12003)
                     /locus_tag="PKN80_02105"
     CDS             complement(11392..12003)
                     /locus_tag="PKN80_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34838.1"
                     /translation="MNGTKRLTALALVLILGLVWFGLANSQENRERRNTDGTTAAGDA
                     QGRGNRSGRWDPQQMNQRRLEWIKTTLQSSDEEWTVLQPRLEKVLTLSRQTGGGRMMM
                     GRSGREGFGGRRSDTRTDTNAARPGNDRTTIQSSPVDKAAGELQQLVSQKDASSGAIV
                     AKLTALRQEKARAKTELAAAQKSLREVLNARQEAQLVLMGLLE"
     gene            12275..12357
                     /locus_tag="PKN80_02110"
     tRNA            12275..12357
                     /locus_tag="PKN80_02110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12308..12310,aa:Leu,seq:caa)
BASE COUNT         2172 a         3716 c         4031 g         2498 t
ORIGIN      
        1 agaaggccga gcagcgggat gtcacccagc agggggactt ttttgacttc ctcggtctta
       61 cggtcctcca tgagtccgcc gatcactatg gtctggccgt cctggatggc cacccggctg
      121 gtcgccgagc gcttggcgaa gaccggggcg tttaccgttt ccgagatcgg caccgtggtt
      181 ccggtcaggg ccgagatctc cggactgacg tcgaggatga ccagtccctc cggattgatc
      241 ttgggcgtca cgttgagaat gatgcccacg tcctggtact ggatggtgtt gatggtctgg
      301 ccggtctcgg tggtgcgggt gttggtaatg aaggggaccc gctgtccgac cgttatctgg
      361 gcccgctggt tctcgctgcc gagtatgtag ggccgggaga gcacgtcgag tttgccgact
      421 ttctgcaggg cggtcagggc ggcggtaacg tctttttcca ccaggctgac gaccattccg
      481 gcgccctggt cgactccgaa cttggtgaag acgcttccct tggcgtcggc gcccaggttg
      541 agtattgaga attcggtgcc caggtccagc gaccggtcgt aggttacctc ggcgatcagc
      601 accttgatca ggacctgggg cagcgggcgg tcgaggtcgt cgatgatctc cttgacccgg
      661 tcgaagttct tcgaggcggt catcaccatc agcgaattgg tgtccgaatt ggccaccacg
      721 taaacctggc cctccaggct gctgccgccg gccgcatcga gggccgccgc cgcggctgga
      781 ttgctcatgc gtatattggt acgctgattt ctggaggcgg tcgaggttcc gaagaggttg
      841 ttcagcgtcg tggccacgtt gttggcggtg ccgttcttga gtttgtaaat gaagacggcc
      901 tcctcggcct ccgggctgga atcgaggtct ttcaccagcg tttcaatcac cttgagcgtt
      961 tcggaggttc cactgaccac gatggtgttg gttcgttcgt cggcggtggc ggtggtctgg
     1021 gcggccgggc ggctgccgtc gctggcggcc ggctgggtgg tcacggttac ctggccgggt
     1081 tgctggccgc cccgtgcggt gaagaagcgg gtggcgccgc tgaccgcgcc ggcctgtccg
     1141 gattggcggg aggcctggct cgaccggaag agttcattga tcaacctggc cgtgttggtg
     1201 gcattggcat accgaagctg gaagaccctg atttcagcta ccgaggaaac ggccgtgtcg
     1261 accgcgttta cgatttccat gatccggcgg atgttggcct cgatatcggt catgatgagc
     1321 gagttgctgc tcgcgttggc gatcaattcg gcgctcgccg gcagcagcgg ggccaggtcc
     1381 tggcgcagtt ttacggcatc cacgtatcga accgggatga tctgggtcac cacgctgtcg
     1441 ctcggttcga tcttgctcgg atcgtttccg atgcggaccg gcaggttccg tttcttggcg
     1501 tcggccagcg ggatgatccg gagcgtgcgg ccggtgacga tcgccgcgta gcccttctgg
     1561 taaagagccg tgttgagcac cgtgatcgcc tcttcacggg tgatcggctg gcggctcatg
     1621 atggtgaggt gtccttcgac ggcaacgtcg cgtacgacga tgaaaccggc ggtttcggaa
     1681 agataggcca ggatggtgtc gagggaggcg ttctggaagt tgagcacgat cccctgggcg
     1741 gccccggtcg gcgggatcgt ttcggggacg aggggtgatg cgggcgtggt ttcccggacg
     1801 gccgtttcag cggaaggtgc ggaggccgga ggcaggctga ccggtggagg cacttccgaa
     1861 ggcagcctgg ccggcgccgg ttcgtttgga aggggcgtcg gcggcatgat cagccctccc
     1921 gatccggggc ccggtatttc ggccgggttg gcacccccgt cgatgatgac ggcttcgatc
     1981 gtggccggtc cggcggcgtc gttggtgatc acgacgccgg ttgcctggtc gggtgccggt
     2041 tgcggattga ctggcggctg ggtggtgtcg tctccctggg tcaggaccga tggaggcggc
     2101 gtataaaccg gcggtgcatt gtcgtctggc aggccgttcg cgttctgggc gtggagattc
     2161 agggttcggc cggccgcggg aagaagcaaa aggctcagcc aggtaatcgc aagggcgcgg
     2221 ttcagtcttg agttacgcat gtggtctcct attcggcgcc gacttccagg cggcgccgct
     2281 gtcgcatctg ttccaggata cgggcttcgt ctatgggagc ggccaccggg taggccgatg
     2341 gcgcgctgct ggccggggtc ggcagagcgt tcttgacggc gggagcgggc ggggccgatt
     2401 cgcgggcaag ggtttccgga accgtttccc cgagccggat ccggttgacc aggccgtctt
     2461 tttcgtattc cacgtaatcg atatttattt ccgcaattct tccacccagg agtgaacttt
     2521 caccgcggac gcgggttgtc atcccgctgg cccggtcttc aaagaaggcc acgaattccg
     2581 aaccgcttcg ggcgatcccg gtaagcacga ccatccggcg gacgatcggg acgaccgggg
     2641 tttctcgaag tatggaagta atccgacctg gttgcggttg ccgattcttg agatagatat
     2701 tccgttccgc caggacccgg taccgctcat aggggaatcc ggttgttgtg ggcgccggtt
     2761 cggccgcccc ggccatccgg ccgccggcgc cgatcgacag gattatccag aggaaaactg
     2821 gtaaaaggcg cctcattccg catctccttc aagggcggcg ttgtttttcg aacgggcctt
     2881 tttggatggt tgcttatccg ccggagccag gcagagggcg gcgacccgga cctggagcat
     2941 caggtcgtcg gaattctcct tgcgccccga caattgcagt tcgcttatct tgatcggcag
     3001 ccgggcgctt tcgattttcc agaggaaatc acggatggcg cgcatcgaac cggcgccgat
     3061 cgcctggaag gaaagttcct ccatctgtcc gatccgggtg cttcgctccg gcttcaacat
     3121 cgaaagggcc aggccgcttt cctcggccca gttgcgcaga gacctcaaag tcaggctttc
     3181 taccgtcccg gcatcagatt ccagcatctt ttcaagcggc gtcggcggtt catcccggcg
     3241 gccaaaaagg gatcgggccg actcgagttc ttcggccagc cgatgttttt ccgacagcag
     3301 ccggccccga cgctccaggt acggggtcaa taaaaaacgg tcggaggcca ggaagaaaac
     3361 ggcgaccagg gtgatgaccg caatgcaacg ctcgcgtttt gagagaatca tcttgttttt
     3421 tccgtcgccg tgcttttccg ggccgaaggt ttgagaaagg caaacctgat cgcgtaggcg
     3481 acttcgggat tgccgccccc ggtttccctt atgtagacaa cgttgacctg cgaaaaggcc
     3541 gggctgcgtt tcagcccttc cagcagatcg aaaaccgccg tgtcccgggc ggctttgccc
     3601 gagatgaggc cctgaaggtc ggccctgatg gccaggttgc tggtccagat gccgccttcg
     3661 gcaggaaagg ccagggtgac ttcacgcagg cactccagga acaagggctc ctggttctgc
     3721 caggcccgca atgatcgcag ccggtcttcc tgcttccggg tgcgggcgag ttccggccgg
     3781 acctcctcca atttctggcg cagtgcgttt atttcccgct ggtgaccgag ggtttcaagc
     3841 cagccgccgg ccgcggtcag gagcaggacc agggcggcgg acgcactcca gatcagcaat
     3901 cgtcgccgga gaggtttctg gtcatctcca aaacgggtca ggcgcggatg caggaaatcc
     3961 agcggcagtg cttgcggacg ttctcccgcc agtgccagcc tgaaggcggc ttcgccggct
     4021 gccggtcggg agccgtttgc catggccggc tcgggtactt ccaggccttt cgttgttttt
     4081 accggcagcg ggagcgcccg ctgcaaggcc tggagggccg tttccggcaa gccggaatcg
     4141 ttccggatgg aaatcgtgct ggcttcgacg aaaccctccc ggcgcggcat caggaaaagc
     4201 agtttttgaa tggccgttcc aatttcaccg gcccaggcag ccgtttcctc attgagtttg
     4261 tccgcggctg gggcgggtat ctttcccaga cgtcggaaaa tgaggggagc gcctccggaa
     4321 agccagacca gttcgacctc ttccggcccc aggtaaagca ccaggttatc cgttcggctg
     4381 ccggcggtgt ccgccagcgc cagtgaggac ggggttgcgg cggccagctt cagaccggcg
     4441 gcatgggcca tctgctgaag cgcttcgatt cgtttccgaa gggccgcgat caggagaatc
     4501 gggcgggagg cgtcagccgc cggcgttgct tcaagatatt ccaggatcag gtccgccggc
     4561 gggaaggcga aatcactctc cgcctccagt tttaaaagat tggcgagcgt cgaagggtct
     4621 gcgggcggga ccgttttttc cttggtcagg agccagccgg ccgggattcc ggcgatggcg
     4681 gtgtcggccg cgatatgctg ggtgcgcagg aattggcgca aggccttccc gaggggcgcc
     4741 ccgatggtca ggccgtccgg gaagggccag cgtacgaccc ggatcaggcg ggacgtccgt
     4801 ccggtcacct ggatttcggc ggcgaccagc gatcgttcgc ccagggtcaa tccgagccgc
     4861 ctggatttcc caaacttaat gaaaggcatc acgaaacacc cccggcgtca gagatggcgt
     4921 tgataaccgg cggttctttg ccggagcgca ggtcattaag aacctgggga tcgagcggcc
     4981 agcccagtcc cgtcaattcc tgccaggcca ggactctggg cgggcgggtg cgggcgtcca
     5041 cgactgcgcg gaaacgcttg aaggcccggc cgtcgccgct taccgccacg atgtcggccg
     5101 agaagcggta ggattgcacg gttatccagt tgcggatggc ggccgctttt gccgccggca
     5161 gcgcctggtt cacccagtcg gggttttcga ggttggtggt ttccgccgcc cgcgcggtca
     5221 gcagggcgtc cacatcggcc tgatccagtc ccggcaggca ctgcaggact tcgcgcggag
     5281 cggtattcac gttgatcggg ctggtcggtc ggtttcgccc gccggtggcc gcggcggcct
     5341 cggagtagac ggttatgaat ggatagatgc cgcggtccag ctggccgtcc cggttgtcgg
     5401 ccggcgggtt ctcgtcgccg tcgtcctcat tgggatcgag cacaccgttg cggttgaggt
     5461 cctctccgaa gagaatctcc gggctcgcgc cttttatcat gagaatctcc tcgatggttt
     5521 ccagcggacc gcttttgcag ttgtaagccg gcgaccggag caggtaatat tcgctcttgg
     5581 caccaccctc gatcgggggc gcctccgcat ccggattgcg ccagtcgatc agggcggccg
     5641 ccagttcttc agtcaggccg ttaaacatca ggagcatctc ctcggtggca taattcaggt
     5701 ttaacctgga ggattcgtcg gtgatgccga aacgatagga ccagtcatcc tccgggtccg
     5761 ggtgcaacag ccagaaataa ccgtttccga ccggaaccgc ctcgcacgat aatgcctcgt
     5821 cgttagacca ggtcccgtcc gtgccgttca cctgagcgat catgaaggcc agccccgccc
     5881 gggccaccca ctccgcctgg gcggcggcca gctggttggc cgaggccagc acttcgaccc
     5941 gcatccggtg cgcaaaaaca agtaccaacc cggccaggac cagtacgacc cacatggtgg
     6001 cgatcaaaat cgtacccctt gaaccgggac gctttttccg gcccgggcgc ggcgcggcgc
     6061 gcccggccag ccgaagctgc cggatcatcc gcctcccgtt ccgcccgtcc gttgggttga
     6121 cgaaccggcc ggtgcggaga cggtttccgc ggcggccggg ttgcagcagg gaagcaggac
     6181 cgtctgccgg aaacggtaca cctccggttc gccttctttc gaggccaggg gaagctcgag
     6241 gctgatttcg accgcccggg gcaggttgtc ggtctggccg gttgagtccc agttgtcgaa
     6301 ccactcgctg ccgtcgaaat agcgcaggtt gaactcgtta acgttccggc agatgacctg
     6361 cgtttccgcc gccgggggct ccggcgccnn nnnnnnnnct gaccgcgcac gatttttggg
     6421 agaacctggt ggccggggtc tccggcgcca gcaggttggt cgtaaccagc ctggtcaggg
     6481 ccggttcaga ctcccctccc gggatctcca gccggtactc gagcccgatg atacccggcg
     6541 ccgcgttcag ccttcccatg ggctgggtga agaaggagat gctgtcgtaa ccgctttccg
     6601 ataatgcctc cccgccatca agaccaagga aaggaccggc caggattccg cgcggaggcg
     6661 gggtcgactg caggtcccgg cgcatgatgt cgaaagcatt ggccgcggcc cggaacggtt
     6721 ccagggattt ttcactttgt tcccgggcgc ggaatccgat tcgaagactg gcgtaaaggg
     6781 aactggccag taaggcggtc atggtcatgg ccaggagcat ctccagcagg gtgaaccctt
     6841 tattgcggca agggttggcg gagggtttat tcatttgaaa ccccctcctc gtagatcagc
     6901 gtggccaggg ttaccgagcg ttccgatccg cgcgattgcc aggtgacttc cacctgcaat
     6961 tcgttaaggt tgctctccgg cccggcggcc actcgcgccg accactggta accgggcgct
     7021 tccggggcga aatcaccgga ggcttcggag aactggttca cgccttccga ttccagctcg
     7081 gccatcaggg tccgggccag gagggtcgct tcaatccggc ggcgcgcgtc cgcggaagct
     7141 ccggtcgcca gagaaacgcc cttcatcgcc accggcagga ggatcatcgt aaatgtcagc
     7201 gccgccagga cttccaccag ggtgaaggca tctctttttt tggcaaggtc gggaatcctc
     7261 atcttatcag gttcctgtcg aagtgaccac ccgaaaacgg tccgtgggcg atgagctggt
     7321 gatatcaagg cggttgtttt tccggtcggt aagccggatg accgccgtct ccgcgctgcc
     7381 gtcgggttga aattcgatat actcgttatc accggagtcc cgtggaacca gggattccgc
     7441 gctgcccatt gaaaggaatt ccgggggact tgcttcggcc gtccgggacg ggtgttcaat
     7501 ccagtcagcc ctggtttcgt cgggcaggcg gaagacccgg cccattccct gcccgagttc
     7561 cttgaaggcg ccgccttcct gggcggtcag ccagaaacgg cccgtttccg tatccaggtt
     7621 caaacgataa acgcgcccct ccgcgaccgc ctgagaacgg gcgtaatcgg acaggctgac
     7681 aaatttgacc gaggcgtcga agatcggccg ggaacgggca aaaccgctca aggaaggtgc
     7741 cgccatcgaa aggacgatgg tgatgaccac catgaccagt atcatttcca gcagcgtaaa
     7801 gccagccgga ggccggccga gatccaccgg gattcgggtt ttaccgttcc gaccagttgt
     7861 cgatatcgtc atcgccgccg tcttttccgt ccgggccgta ggagtaaagg tcataactgt
     7921 tggaattgtt atcgcccggc gaggtgtaaa cataggggtt gccccaggga tccttcggca
     7981 ctccccgctt gatgtaaggc cccttccagt tttcaagctg ggccggcacc tcgaccaggg
     8041 cctcgagtcc ctcttccgtg cttggatagc ggccggcgtc gacttcgaag aggtccagcg
     8101 ccgcttccag gctggcgatg tcggtgctcg cggcggtgat gcgggcctgc tcggaacgct
     8161 tggcatacct gggaaccacg atggccgcca ggatcgtcag gatcaccagg accagcagaa
     8221 gttcgatcag ggtgaagccc cggttaccgg accgtccaat ggagtaattg tccggtttga
     8281 cgcgtttaca gggtttgctt ggcatgactg tctcctcctt ttaataattt gagatggccg
     8341 ggcttcaacg gatgaggtcc tgaagcgtaa agaccggcag gagcattccg attaccaccg
     8401 tgccgacgat gccggccgtc aaaaacagga gtaccggctc gatgaccgaa accagcatgc
     8461 gcaactggcg atccaggtca ttctcgtacg aggtggccag gcggatcaat tccttgttaa
     8521 ggttgccgct ttcctcggcg acggccacca tttccacgac cgagggcgga aagaggcgtt
     8581 cgcttgaggc cagccccatg gccaggctcg atccgctttt gacgttttcg accgcgcgcg
     8641 tcaccgcgtc ggagagcgtc cggtttccga tggcttcgcg cgcgacgtta agggcgagca
     8701 ccagcggtac gcccgcgccc agcagggtac ccagcatgcg ggagaagcgg accagggcaa
     8761 acctggccac cacggtgccg atcccgggca atttcaggac ggtccgttcc aggacgcgct
     8821 gcccggtttc ggtggcgagg gcccggcgca gggaaatgat gcccgcgacg gccgcggccg
     8881 ccaggatcag tccgtgccct accagccagc ggctgaggtt cattatcaac ctggtcagcc
     8941 agggcagcga acctccgaac tctgagaata tcgccgaaaa acgaggaatg aaataggtca
     9001 tcaggaagat gagtaccacg ccggccagtc cggccagcag caccgggtaa accagcgccg
     9061 ccttcacccg ccccttgagg tcatgttccc gggcgcggaa atcggctatc tgttccagcg
     9121 ccaggttcag gaaaccgccg gtctccccgg cgcgtactat ggcaacataa accgggggga
     9181 aggatcgcgg ccacttgctt aaagcggtcg cgaaggagag gccgcccttg atgtcgttcc
     9241 ggatcgtgtt ccattgctgc cggacgtccg ccgccgacgt ttcccgggtt acgatgtcca
     9301 gggaacggct cagcggaaca ccggcggcca gcaggttggc cagctggcgg gtgaaggctt
     9361 cgacgccggt ctgcgaaccg gccgtccggc tgaaaaggcg ccgtcgaatt cccaccccgg
     9421 ccggtgcctc ctccgccccg atttccttca ccgagaccgg ccgcaggccc tgccgtccga
     9481 tcgcttcgag gagctccgaa cggctgccgg cggtcagcgt gccggagacc ttttcaccgg
     9541 agacgttcag agcctggtac gagaaaacgg gcattttgtt cttccgttcg gaccgtcgcc
     9601 tattccagga ccggtccgga ttcattcagc tcaccgggcg ccgtcccgta ggagttggtc
     9661 ccgtttaccc gttcatcctt ggtaacgcgg ataacctcct caggcgtcgt aaaaccttcg
     9721 cttatcaatc gccagccgtc ttcccggagg ctgcgcatgc cctgccgggt cgcggcggcg
     9781 cgtatttcgc gcgaagaggc ccgcttgaga atgagggcgc ggatatcatc ggtcacgtgc
     9841 atgaattcga agacgccgct gcgcccgagg tagccggtgc cctggcattc gcggcaaccc
     9901 cgcccccgaa agagcaccgg cgggatttcc tcgccatatt cccggcgcag gacctcccgg
     9961 tcggcgtcgg ctatctcctc tttgcagtgc gggcagatca ggcggaccag gcgctgggcc
    10021 aggaccgcct ccagggacga ggcgaccagg taaggttcga cgcccatgtc caccagccgg
    10081 gttggagccc ccggcgcatc gttggtatgc agggttgaga agacaagatg gccggtcaac
    10141 gaggcctgga cggcaatttc ggccgtctcg taatcgcgga tctctccgac caggatgaca
    10201 tccgggtcgt ggcgcaggat ggcgcgcagg cccgaggcga aggtcaggcc ggccttgttg
    10261 gaaacctgaa tctggttaat gccccggaga tggtattcga tcggatcctc gatggtgacg
    10321 attttgcgct ggatgtcgtt gatcgtggat agaccggcgt aaagcgtggt ggttttgccg
    10381 ctgccggtcg gtccggtgac caggatgatc ccgtggggaa ggtccagcat ccggccgaag
    10441 aagttccggt cccgttccga cattccgagt tgggccaggc ccagcagggc ggcgttccgg
    10501 tccagcagcc gcaggacaat cgcttctccg tgcagcatcg ggattaccga gacccggacg
    10561 tcaatctccc gcccggagac gaaaatcttt atccggccat cctggggcag ccttttttcg
    10621 gcgatgtcga gcgaactgac tatcttgaga cgggagacga ttgccgcctg gaagcggcgg
    10681 atctcgggcg ggacggcggc atccttcaaa accccgtcga tccggtaccg cacgcgcaat
    10741 tcgttttcaa agggctcgaa gtgaacgtcg gtcgcccgca gttcgatcgc ctccttgaga
    10801 atctggttga cgaaccggat gatcgaggcg tcctcggcgg ccgccccgag gtccaccccg
    10861 gattcggtct cgtcggtgac cacggtgatc ggcatctcct cactgtcgga aatcatcgac
    10921 tgcaccgtgt cggcgccgat tccgaggcag atcttgagcc cctgccggat ctgggaaggc
    10981 gtggtcagaa ccggcttgaa ttcgcgcccg gtggccaggc gcagctcgtc gatgccggcg
    11041 gtgtcgaaga ggcggctggt agcaaccatt accgtgccgg cctcttcgga gaggggcagc
    11101 agctggcgat caatcaaaat gcgggccggg aaaaccgcaa ggaattcccg gcccggaaga
    11161 gattcttcga tatcgcggta ggaaacgttg aattcggccg ccaggatttt cagcaaggtt
    11221 tcttcggcca ggccggatcc ggtcagcagc gcttcatcaa gcggaacgcc cgatttgctt
    11281 aaggaacgga cccgttcggc cgtttcccga ttcaacagtt tgttttcgat taatttaccg
    11341 acgatatcga tcatgccttt tttttcgatt cgcgagagag ttgaagcgag attattcgag
    11401 cagacccatc agaaccagct gcgcctcctg acgggcgttc aggacttccc ggagcgattt
    11461 ctgcgcggcg gccagttcgg ttttggctcg ggccttctcc tggcgcaggg cggtcagctt
    11521 ggcgacgatc gccccggaag aggcgtcttt ttggctaacc agttgctgca gttcgccggc
    11581 ggccttatcc acgggcgaag actggatggt ggttcggtcg tttcccggcc gcgccgcgtt
    11641 ggtatcggtc cgggtgtcgg aacgccggcc gccaaaacct tctctgccgg agcggcccat
    11701 catcatgcgg ccgccgccgg tctggcgcga aagggtcaag accttctcga gacgcggttg
    11761 taaaacggtc cattcctcgt cacttgactg gagagtcgtc ttgatccact ccagccggcg
    11821 ctggttcatc tgctggggat cccagcgacc ggagcggttg ccacgtccct gcgcatcacc
    11881 ggcggccgtc gttccatcag tgttccggcg ttcccggttt tcctgcgaat tggccagccc
    11941 gaaccagacc agacctaaaa tcagcaccaa cgccagtgcc gtcaaccttt ttgttccgtt
    12001 catagccacc ctcccctgtt gctgagcaac cgtttgcgaa aaaagccccc catagctgac
    12061 tttaatatat gatgaccgga tgggactgaa cgtttaaagg ccggccgtca gcttggattg
    12121 gaagatttcc ttaaatgtga aattggattt tggaaggaat gggctgcctc atcctactcc
    12181 cggaatactg atggtgttag gtcctggcgt atatgataaa atatcaattc caaatgagac
    12241 gctggaacat ttccagggaa tcgctgcaat agaagccgga gtggtggaat ggcagacgcg
    12301 ccggactcaa aatccggtga gggcaacctc atgagggttc aagtccctcc tccggcaccg
    12361 cttcctgaca aaggacttga agagagcgaa gtcaggcaag gcgaccgaat agggagcgtt
    12421 gccagcg
//
LOCUS       DAOQYN010000027        11988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_5815_length_11988_cov_4.185033, whole genome shotgun sequence.
ACCESSION   DAOQYN010000027 DAOQYN010000000
VERSION     DAOQYN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11988
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5815_length_11988_cov_4.185033"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1984)
                     /locus_tag="PKN80_02115"
     CDS             complement(<1..1984)
                     /locus_tag="PKN80_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34839.1"
                     /translation="MTNKSIARRLAWLGMAALCLAGGRTAFADDAPKSDSKPMAAQNI
                     LWNASFECGVGSGTWGVIRRVGPNVPESWHRGGYRSAYCLSLVKPIGSRLYKIGDKPG
                     TWRLTLMARSLSGTARLVVDLNNNNKPGSDKEGGINYYRRAFEVGEEWQSFSMDVQVG
                     EVVRPYFHLELSGEGVLVDEVSLYRLEDPAAKSVAGQPIQSVETGFVVPEITKVYLDG
                     EKRLCRLLVTNNTEGPIETNVSYSIFDAFENPVRTEKVAVKLAGRETREFPVDLENLK
                     YGAYRLRSSWGEAPAQGDALVGLLPAVRHDLPTRWGCNASLDDPSLDFSIRMMKRLGM
                     GFVSTLSTGSYLGRWSLVEPEPGQLKTRLDIARKLKENDIWIAAYLQAAEWPSWLIQK
                     KPTDQEVIEAFGKYVAAYIKAYEPYVKLFHIEDETERKSQLQKLDFYAALHKHAYDTA
                     KKTAAELGREILFSTNTVDVGWFGQIWDKIGEGYFDFLSINTTHLPTWTVRLLREQAK
                     RNSHERILFSPAIGQKCTMRQTTLIQDTPSSGVPPTTFVWQALKTSWLSRPYGLEAGI
                     FAPEIHFGFYEIRVHDVAHYIPRQGWTGLEYDNSPHAGFQAVTAMRSLIGPMQPVRRT
                     LGTEVSEAGMPLGTNQDFEAYPFRNQTGAAVAFMTAS"
     gene            complement(2259..3023)
                     /locus_tag="PKN80_02120"
     CDS             complement(2259..3023)
                     /locus_tag="PKN80_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNQ34840.1"
                     /translation="MLAGCATSTSSVNKDFSWWGKSGVGPAPMLDPDTMVPSVPEKSG
                     SWWMPDPTAVSPDDASLWGNRGYVYVAEIAEPTTPAPKVEAPAPPAPKPVERVVEKEV
                     VRTVEKIVEKPVEKIVYVDKPVEKIVYVDKPVEKIVERPVTAKAVYIDIQDVYFPYDS
                     AELTAFNRGILDHNIEVFKKYPNLKTILLGYASPEGSSEYNLKLSERRVMAVKNYLVK
                     KGVPENVFAVKKMGEMEMPQPSWPFARKVHFEIVSD"
     gene            complement(3305..4144)
                     /locus_tag="PKN80_02125"
     CDS             complement(3305..4144)
                     /locus_tag="PKN80_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNQ34841.1"
                     /translation="MKPAICAPFDYETPLDRAIPMIRTAGFEVISIGGRPRHPDYTSA
                     AGRKTIRKLVKSGGLDIESVHAPFPEGDRLFSLDEAERLESIRQCQAALDAAAELEGR
                     TVVIHLIQPYGIPPGETRDRMVEAGRQSVEALADHAGSRGVKLALENGQRADYDAVLR
                     DFLLEFKADHVGFCYDSGHENVQGTCFKILEEFGSRLLTVHIHDNLGSDTHLLPYEGN
                     IDWAKFRRVFFSLSYSGHLLLESGLANSRFQEPERFLAEARERAERIINGSGIPTADP
                     HQE"
     gene            complement(4281..4970)
                     /gene="aqpZ"
                     /locus_tag="PKN80_02130"
     CDS             complement(4281..4970)
                     /gene="aqpZ"
                     /locus_tag="PKN80_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNQ34842.1"
                     /translation="MDRRVGAEFLGTFWLVLGGCGSAVLAAAVPELGIGFAGVALAFG
                     LTVLTMAFAIGHISGCHLNPAVSIGLWAGKRFPGRDLPPYILAQLAGAIAAGLVLFII
                     ASGKAGFDPLNGFASNGYADRSPGGYSLLSCFITEVVMTAVFLVVILGATDRRAPQGL
                     APVAIGLALTLIHLISIPVTNTSVNPARSTGVALFAGSGALKQLWLFWLAPIIGAMLG
                     GTIYNALGRED"
     gene            5204..6715
                     /locus_tag="PKN80_02135"
     CDS             5204..6715
                     /locus_tag="PKN80_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNQ34843.1"
                     /translation="MDRILIGKGDQPVYLLSRYGNRHGLVAGATGTGKTISLLVLAEG
                     FSRMGVPVFMTDVKGDVAGLAMAGLANEKIAQRVSQIGIDGYANEANPVIFWDLYGQA
                     GHPVRTTISEIGPVLLGRILELNDTQVGMLEIAFKLADDNGLLLLDLDDLRSLLGFVS
                     DNRNEISRQYGLVSTQSIAAVQRSLLSLEREGGTAWFGEPALELNDLLRTDLNGRGII
                     NVLAADRLILKPRLYSSLLLWLLSELYENLPEVGDLDRPRQVFFFDEAHLLFNDAPPV
                     LLQKVEQVVRLIRSKGVGVYFCSQFPDDVPSEILGQLGNRIQHALRAYTPRDQKAVRT
                     AAETFVANPALDVAEAISRLGVGEALVSTLQEKGVPMPVERTLICPPRCRMGTLTPEE
                     RILIRSRSPIGGKYDSPVNRESAYEILSRRALEKESAPEPAPAPGKGGGRSGEEKADG
                     RGSLIGDLLWGTKRRQGMVETLAKQTVRTVGSQIGREIIRGVLGGILGGSRRR"
     gene            complement(6781..7251)
                     /locus_tag="PKN80_02140"
     CDS             complement(6781..7251)
                     /locus_tag="PKN80_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34844.1"
                     /translation="MIIIKPHHLVDIITDFGAGITDPEPHPYGHAVHSVTREILANPD
                     VELGMEWGADDICRPCRHNLEGLCDDVIDTSYRPAAPKSKREWNLLLDRRWSERLGLR
                     PDERLTAREFCARLRDRAGDVSDIYRELPGDRIATRQANLELGVRKLLEKPAKA"
     gene            complement(7327..8739)
                     /locus_tag="PKN80_02145"
     CDS             complement(7327..8739)
                     /locus_tag="PKN80_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNQ34845.1"
                     /translation="MAGRKNPDIIVIVSDSLRQDHVSYYAGQDSPAPTPHIDALLKEG
                     VAFDNMYPEGLPTIPVRTEWVTGNGTLTGRSWQPLADTDVTAAEILRREGYLTALITD
                     VYHYFKPGQNFHRGYHEWQWIRGQEYDACRTTPPRKRRLEDHWKETFPAAWRGPVQIV
                     CQNLDEARLARDFPCYRTFETAADWLRRNRGQSQPVFLWVETFDPHEPWTPPAEFDTF
                     KDPAYRGKDFILPPGGEAAAYFSPEEIKRIRSLYAGEVAYVDAMVGEFVKALKENGRY
                     ESSLIFFLSDHGHPLADHGKFLKGPDRLHSELLKVPFGFKLPGGEYGGRRLTRIAQFP
                     DVLPTLLDAAGLGNNTFDMQGGSLLPLVRGEVEMVRKAAVSGYHKGQDRCIRNEKWSL
                     VLRPEGQPDELYDLEKDPRETRNLIEAHRETALELKAHFGAAYTGASRPVKGLQGSFE
                     VADTAATGDLNAQKRESKKN"
     gene            9025..9984
                     /locus_tag="PKN80_02150"
     CDS             9025..9984
                     /locus_tag="PKN80_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNQ34846.1"
                     /translation="MRKTRVLIGAILLLALSIPAFGADQTGNDTGLVRKTLTHGGRRR
                     SYSLHLPGNAVPVRPLPVLFVLHGGDGAGAQVMASGSGYNEIADRENFIVVYPFGLQG
                     QWNDGRGKSFRRRDNSSVDDVGFIGAVIDELFREFSIDRRRVYVMGVSNGGMMTLRLG
                     IELGDRVAAIAAVVANLPDRLASARPVRPLPVLIMNGTRDPLVPWKGGAVRVFGKDYG
                     DVLSTESTVAFWVKANGLNGPVEKNQLPDRVSTDGCRVEVSAYRSEGDRPPVILYAIQ
                     GGGHSFPGARTLLRYILLGNKCMDINGAQVIWDFCKDYSLQGE"
     gene            10067..11215
                     /locus_tag="PKN80_02155"
     CDS             10067..11215
                     /locus_tag="PKN80_02155"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNQ34847.1"
                     /translation="MKKRITLLILVLLVVPAILAGIIQILANPLAKKAVEARVGPLFE
                     GRLSIGSVRISVPGRSIILRNLLLRQPPGFPPGNLLTVDEIRVQAAFAPLLRRRLVIN
                     RLLISNPEINIIQAAGGATNLEHDLGRFAVKKGGKEPPAFKFHLDGLSIRGGRVNLYS
                     RQLSPAKEPALSVSRLGLTLGNLNFPNEERLESPFKTQAVIETVNPVPVAVRGRIVAG
                     AGPLDFDASTEIRGVNLADFAYLYPAAPISITAGRADVRAEARCRQNDLESRQHLEIR
                     GLKLAGSGGFFKKTLLGLPAAAAVKFLEDREGALSLDFEVKGTVNELKSDLKEAIAAS
                     FSRAFREKFGRPILAVDRLIGGAARGLWTGIGGGLRKAGKGISNLMGR"
     gene            11249..>11988
                     /locus_tag="PKN80_02160"
     CDS             11249..>11988
                     /locus_tag="PKN80_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34848.1"
                     /translation="MIIRKLCLAVAGSFPFFLCFSVWPVPAFGQEMRPAIKNPYYEKP
                     AWKLWCDDPAPVGDVSLPPEAQRLFPWPPASERFVDYRKGQDWPGRTYAGFWCDVYSS
                     EDNKHSYVRDAWKSPAGDNAVEGILEGMIHLNRGEIGSHGINFTSQSTTEMGHHIANE
                     KDRLERTERDYYFRDCLRTGPAHISFMDRAPDRAVDEYQALAPCFYNSIGSSGSETMA
                     ITKMLIAGGYLPVALKRTLQVNGLYPATL"
BASE COUNT         2087 a         3632 c         3808 g         2461 t
ORIGIN      
        1 cactggccgt catgaaggcc acggccgccc cggtctggtt ccggaaaggg taggcctcga
       61 aatcctggtt ggttcccagg ggcatcccgg cctccgagac ctccgttccc agcgtccggc
      121 ggaccggctg catcggtccg atcagcgagc gcatggcggt aaccgcctgg aagccggcgt
      181 gcggggagtt gtcatattcc agccccgtcc agccctggcg cggaatgtag tgggcgacgt
      241 cgtggacccg gatctcgtag aagccgaagt gaatctccgg cgcgaagatg cctgcctcca
      301 gtccgtacgg gcgggagagc cagctggtct taagcgcctg ccagacgaag gtcgtgggcg
      361 gcacgccgga actcggcgtg tcctggatca gcgtggtctg gcgcatggtg catttttgtc
      421 cgatcgccgg gctgaagagg atcctttcgt gcgagtttcg tttggcctgt tcgcggagca
      481 gccggacggt ccaggtgggc aggtgggtgg tgttgatgga gagaaaatcg aagtagccct
      541 ccccgatctt gtcccagatt tggccgaacc agccgacgtc gaccgtgttg gtcgagaaga
      601 ggatctcccg gcccagttcg gcggccgtct tcttggccgt gtcgtaggcg tgcttgtgca
      661 gggcggcgta aaaatcaagt ttctgtagtt gtgatttgcg ttcggtttcg tcttcgatat
      721 gaaataattt gacgtatggt tcgtaggcct tgatgtaggc cgccacatac tttccgaagg
      781 cctcgatgac ttcctggtcg gtcggttttt tctggatcag ccagctgggc cattcggcgg
      841 cctgcaggta ggcggcgatc cagatgtcgt tctccttcag tttccgggcg atgtcgagcc
      901 gggtcttcag ctggcccggc tccggttcga cgagtgacca gcggcccagg tagctgccgg
      961 tgctcagggt cgaaacgaaa cccatgccca gccgtttcat catgcggatg gaaaagtcca
     1021 gcgacgggtc gtccagcgag gcgttgcagc cccaccgggt cggcaggtcg tggcggaccg
     1081 ccggcagcag cccgaccagg gcgtcgccct gggccggggc ctctccccag gaactgcgca
     1141 ggcggtaggc cccgtacttc agattttcga gatcgaccgg gaactcccgg gtctcccggc
     1201 cggccagttt caccgccacc ttttcggtcc ggaccgggtt ttcgaaggcg tcgaagatgc
     1261 tgtaggagac gttcgtttcg atcggtcctt cggtattgtt ggtcaccagc aggcggcaga
     1321 gtcgtttttc accgtcgagg taaaccttgg ttatttcggg cactacgaaa ccggtctcca
     1381 ccgactggat cggctggccg gccaccgatt ttgccgccgg atcctccagc cggtacagtg
     1441 agacctcatc caccagcacg ccttccccgg agagttccag gtgaaagtaa ggccggacga
     1501 cctcgcccac ctggacatcc attgaaaatg actgccactc ctcgcccact tcgaaggccc
     1561 ggcggtaata gttgatgccg ccctccttgt cggaaccggg tttgttattg ttgttcaggt
     1621 cgacgaccag cctcgccgtt ccggagaggc tccgggccat cagcgtcaag cgccaggtcc
     1681 ccggcttgtc gcctatcttg tagaggcggg aaccgatcgg cttgaccagc gaaaggcagt
     1741 aggcgctccg gtagccgccg cgatgccacg actccggtac gtttggcccg acgcgccgaa
     1801 tcacgcccca ggttccggag ccgactccgc attcgaatga ggcattccag agaatgttct
     1861 gcgcggccat cggcttggaa tcactcttgg gtgcgtcgtc ggcaaaggcc gtccggccgc
     1921 cggccaggca gagcgccgcc atgcccagcc aggccagccg acgggcgatg cttttgttgg
     1981 tcatgatttc cccccagtgt ggtggacatc gaggtttttg atgagttgat cttggcgaag
     2041 gtttggattc caaggctttt cgtgtttccg tcattatagt gtaatgcctc cgggaatgtc
     2101 aacgcagttt tggaaattcc gtaaacggat aatggcgagg cgggaattcg tattggccgg
     2161 tggccccaag gtcttctttt gaaaaattcc gctcaataaa aaagcccccg cccggggttt
     2221 cgcccgggac gggggccttt cttgcctgat ttggagcttc agtcggagac gatctcgaag
     2281 tgcaccttcc gggcgaacgg ccaggagggc tggggcatct ccatctcgcc catcttcttg
     2341 accgcgaaca cgttttccgg aacgcccttc ttgaccaggt aattcttgac cgccattacc
     2401 cggcgttcgg agagcttcag gttgtactcg ctggatcctt ccggggaggc gtagccgagc
     2461 aggatcgtct tgaggttcgg atacttcttg aagacctcga tgttgtggtc caggatcccg
     2521 cggttgaagg ccgtcagctc ggcgctgtcg taggggaaat aaacatcctg gatatcgatg
     2581 tagaccgctt tggcggtcac cgggcgctcc acgatcttct cgaccggctt atcgacatag
     2641 acgatcttct cgaccggctt gtcaacgtaa acgatcttct cgaccggctt ctccacgatc
     2701 ttctcgaccg tccggacgac ttccttttcc acgacgcgtt cgaccggctt gggcgccggg
     2761 ggtgccgggg cctcaacctt tggggccggg gtcgtcggtt cggcgatttc ggccacgtaa
     2821 acataaccgc ggttgcccca gagcgaggcg tcgtccgggc tcacggccgt cggatccggc
     2881 atccaccagg aaccgctctt ctccggaacg ctggggacca tggtgtcggg gtcaagcatc
     2941 ggcgccgggc cgactcccga tttgccccac caggaaaaat ccttgtttac gctcgaggtg
     3001 ctcgtcgcgc atccggctaa catggcaacc gataagacca ccaaccccaa taccgcaaaa
     3061 cgcatccttc ccatgccccc tccttatttt tattttgcga cccggcatcc tacggtcgcc
     3121 ttacttttat aatataagat ttgttataca tttgtcaagt ccaatgattg gaacgcaagg
     3181 ccaattttta aagcaggagt aagaatttgc tacttttagt ttttgatgtt gttcaattct
     3241 caatcttgaa atgtatgtat ataccaaaaa gaagtggcgg ggatgttttt caggatgtca
     3301 tcctttactc ttgatgggga tcggcggtcg gaattccgga accgttaatg atccgctccg
     3361 cccgttcccg ggcctcggcc agaaaacgct ccggctcctg gaaccgggag ttggcgaggc
     3421 cggattccag caggaggtgg ccggagtagg aaagggagaa aaagactctt ctgaattttg
     3481 cccagtcgat gtttccctcg tacggcagca ggtgggtatc cgaacccagg ttgtcgtgga
     3541 tgtgaacggt caggagacgg cttccaaact cctcgaggat cttgaagcag gtcccctgaa
     3601 cgttttcgtg gccggaatcg tagcagaacc cgacgtgatc ggccttgaat tccagcagaa
     3661 aatccctcag gaccgcgtca taatcggccc gctgaccgtt ctcgagggcc agcttgacgc
     3721 cccgtgaccc ggcgtgatcg gccagcgctt ccacgctttg gcgccccgct tcgaccatcc
     3781 ggtcccgcgt ttcgcccggc gggatgccgt agggctggat caggtgaatg accacggtcc
     3841 ggccttccag ttccgcggcc gcgtccaggg cggcctgaca ttgccggatg gattcaaggc
     3901 gttcggcctc gtcgagcgag aagaggcggt caccctccgg gaagggggcg tggacggatt
     3961 cgatgtccag gccgccggac ttgaccagtt tgcggattgt tttccggccg gccgcgctgg
     4021 tgtaatcggg atggcggggc cggccgccga tcgagatgac ctcaaacccg gccgtccgga
     4081 tcatcgggat ggcccgatcc aggggagttt cgtagtcgaa aggggcgcag attgccggtt
     4141 tcattttata tccatagggg ttttatttga ataattccag cccgggtggc caggaaacgg
     4201 cctgtttcct tgccggagcc gacgggaacc ggggaaattt gctgtccgaa acaagatgag
     4261 gtttccgatc cggttggagg tcaatcctcc cggccgagcg cgttgtagat ggtcccgccg
     4321 agcatcgccc cgatgatcgg tgccaaccag aagagccaga gttgtttcag cgccccgctg
     4381 ccggcgaaga gcgcgacccc ggtgctccgg gccgggttta ccgaggtgtt ggtaaccggg
     4441 atgctgatca ggtgtatcag cgtcagggcc aggccgatgg ccaccggtgc cagcccctgt
     4501 ggtgcccgcc tgtcggtggc cccgaggatg accaccagga agaccgccgt catcaccacc
     4561 tcggttatga agcaggataa gagcgaatat ccgcccggcg aacggtcggc gtagccgttg
     4621 gaagcgaagc cgttcagcgg gtcgaatccc gccttgccgc tggcgatgat gaagaggacc
     4681 agcccggcgg cgatcgcccc ggccaactgg gccaggatgt aaggcggcag gtcccggccg
     4741 gggaagcgtt ttccggccca gaggccgatg gaaacggccg ggttgagatg gcagccggaa
     4801 atgtgtccga tggcaaaggc catggtcagg accgtcaacc cgaaggccag ggccaccccg
     4861 gcaaaaccga tgccgagttc cggtaccgcc gcggccagga ccgcgcttcc gcatccgccc
     4921 aggaccagcc agaaggttcc caggaattcc gcgcctactc ttctatccat gccccccctt
     4981 ttccggatct gcttgaccgg gacgcttgtt gccgggtagc cggccgcttt taattaataa
     5041 aatgccacca ttgccggact ttgtcaaatc agccggctgg tttccgccgg ttccctttcc
     5101 ttccatccgc tcccggggtt atactatttc cgagggggcg tttttcggat gacccgttgc
     5161 agttcaatgc gggtgttgag gctttcatga agggggggca ggcatggaca ggattctcat
     5221 tggaaagggc gaccagccgg tttacctgct ttccaggtac ggcaaccgcc acgggttggt
     5281 cgccggcgcc accggcaccg gcaagaccat ctccctgctg gtgctggccg agggtttttc
     5341 ccggatgggc gtgccggtct tcatgaccga tgtcaagggc gacgtcgccg gcctggcgat
     5401 ggccggcctc gccaatgaaa agatagcgca gcgggtcagc cagatcggca tcgatggata
     5461 cgcgaacgag gcgaatccgg ttatcttctg ggacctttac ggccaggccg gccacccggt
     5521 gcggaccacg atcagcgaga tcggtccggt cctgctgggc cgcatcctcg aattgaacga
     5581 tacccaggtc gggatgctcg agatcgcctt caagctggcc gacgacaacg gcctgctgct
     5641 actcgacctc gacgatctcc gttccctgct cgggttcgtt tcggacaacc ggaacgagat
     5701 atccaggcaa tacgggctgg tgagcaccca gtccatcgcc gccgtccagc gttccctgct
     5761 ttcgctggaa cgggagggcg gaaccgcctg gttcggagag ccggcgctgg aactgaacga
     5821 cctgctccgg accgatctca acggccgtgg catcatcaac gtcctggccg ccgacaggct
     5881 tatcctgaaa ccgcgcctct attcgagcct gctgctctgg ctgctttcgg aactctacga
     5941 gaacctgccc gaggtgggcg accttgaccg tcccaggcag gtcttcttct tcgacgaggc
     6001 tcacctgctc ttcaacgatg cgccgccggt cctgttgcag aaggtggagc aggtcgtgcg
     6061 gctgatccgt tccaagggtg tcggagttta cttctgttcg cagtttcccg atgacgtgcc
     6121 ctctgaaatc ctgggccagc tgggcaaccg cattcagcac gccctccggg cctacacgcc
     6181 gcgtgaccag aaggcggttc ggacagcggc cgagaccttc gtcgccaatc ccgccctcga
     6241 cgtggccgag gccatctccc ggctcggggt cggcgaggcc ctggtctcga cactccagga
     6301 aaagggggtg ccgatgccgg tggaacggac cttgatctgc ccgccccgct gccggatggg
     6361 aacgctgacg cccgaggaac gaattctgat aaggtcgcgc agcccgatcg gcggcaagta
     6421 cgattccccg gtcaaccggg aatccgccta cgagattttg agccgccggg cccttgagaa
     6481 ggaatcggcg ccggaaccgg ccccggctcc gggaaagggc ggcggccgga gcggcgagga
     6541 gaaggcggac ggacgcggga gcctcatcgg cgacctgctt tggggtacca agcgccgcca
     6601 gggaatggtg gagaccctgg ccaagcagac cgttcgcacg gtgggaagcc agatcggtcg
     6661 ggagataatc cggggcgtgc ttggcggcat cctgggcgga tcccgccgtc gttaggggcg
     6721 gggagggcgg ttcggtccgg aagcttaagc ttttggttgc cgtccccggc ccggaagtcc
     6781 ttatgctttg gccggctttt ccaggagttt ccgaacgccc agttccaggt tggcctgccg
     6841 ggtcgcgatc cgatccccgg gcagttcccg gtagatgtcg ctgacatcgc cggcccggtc
     6901 gcggaggcgc gcacagaatt cccgggccgt cagccgctcg tccggccgca gcccaagcct
     6961 ttccgaccag cgccggtcca ggagcaggtt ccattcccgc tttgacttcg gggccgccgg
     7021 acggtaggag gtgtcgatca cgtcgtcgca gagaccctcg aggttgtgac ggcagggccg
     7081 gcagatgtca tcggcgcccc attccatccc gagttccacg tccgggttgg cgaggatttc
     7141 cctggtgacg ctgtgcaccg cgtggccgta aggatgcggt tcgggatcgg tgatcccggc
     7201 tccgaaatcg gttatgatgt caaccaggtg atgcggcttg ataatgatca tccttagcct
     7261 tcctttcctt ggccggggcg gtttttcgcc ggttcgggcg gtgacgaagg ccccggttcc
     7321 ccgtttttaa ttcttcttcg actcccgttt ttgcgcgttc aagtccccgg tcgccgcggt
     7381 gtcagccact tcaaaggagc cctgcaaccc cttgaccggc cggctggcgc cggtataggc
     7441 ggcgccgaaa tgggccttca actccagcgc ggtttcgcgg tgcgcctcga tcaggttccg
     7501 ggtctcccgc gggtcctttt caaggtcgta cagctcatcc ggctgtcctt ccgggcgcag
     7561 gacgaggctc catttttcgt tgcggatgca gcggtcctgc cccttgtgat agccggagac
     7621 ggcggccttc ctgaccattt ccacctcgcc ccggaccagc ggcagcagcg aaccgccctg
     7681 catgtcgaag gtgttgttcc cgaggccggc ggcatccagc agggtcggga gcacgtccgg
     7741 gaactgggcg atcctggtta gacgccggcc gccgtattcc ccgccgggca gcttgaaccc
     7801 gaagggaacc ttgagcagtt cggaatgcag ccggtccggc cccttcagga acttgccgtg
     7861 gtcggccagc ggatggccgt ggtcggagag gaagaagatc aggctgctct cgtagcgccc
     7921 gttttctttc agggccttca cgaactcgcc gaccatggca tccacgtagg ccacctcgcc
     7981 ggcgtaaagc gagcggatcc gcttgatctc ctccgggctg aaataagcgg cggcctcccc
     8041 gcccggcggc aggatgaagt ccttgccccg gtaggccggg tccttgaagg tgtcaaactc
     8101 cgccggcggc gtccagggtt cgtggggatc aaaggtctcc acccagagga agaccggctg
     8161 cgactgcccg cggttgcggc ggagccagtc ggcggccgtc tcgaaggtcc ggtaacaggg
     8221 aaagtccctg gccagccggg cctcgtcgag attctggcaa acgatctgta cgggaccgcg
     8281 ccaggcggcg gggaaggtct ccttccagtg atcctcgagc cgccgtttcc gcggcggggt
     8341 ggtccggcag gcatcgtact cctggccccg gatccactgc cattcatggt atccccggtg
     8401 gaagttctgg ccgggtttga agtagtggta aacatcggtg atcagggcgg tgagataccc
     8461 ttcgcgtcgc aggatttcgg cggcggtcac gtcggtgtcg gccagcggct gccaggagcg
     8521 tccagtcagg gtgccgttgc cggtgaccca ctcggtgcgg accgggatgg tcggcagtcc
     8581 ttcgggatac atgttgtcaa aggcgacgcc ttccttaagc agggcgtcga tgtgcggggt
     8641 cggcgccggc gaatcctggc cggcgtagta actaacgtga tcctggcgaa gcgagtcgga
     8701 tacgatcacg atgatgtccg gattttttct gccggccatt ctggctcctt tttaaatcaa
     8761 tgtttaaccg gttgacgcgg aaaaattttc cgggtgctga gagtttggga aaggcggtcc
     8821 tggtacttca agatatttta tttaaccgga atgggggtgt caaacggacc gtttctttcc
     8881 tgatccggtc cttcccgttt taactgtccg cgcttcggga aggtttgaca gcggcccgga
     8941 aaaatcataa attttaagag aagcacgcag ccgttggaga gtttaatgag gataaagaag
     9001 cgtttaaccg aaaggatggg cggcatgagg aaaactcggg ttttgattgg cgcgattctc
     9061 ctgctggcgc tgtcgattcc ggccttcggc gccgaccaga ccgggaatga caccggcctc
     9121 gtgcgcaaga cgcttacgca tgggggacgg cggcgcagct attccctcca cctgcccggc
     9181 aatgccgttc ccgtccggcc gcttccggtc ctcttcgtgc tgcacggcgg tgacggggcc
     9241 ggcgcccagg taatggcctc cggcagcggt tacaacgaga ttgccgatcg ggaaaatttc
     9301 attgtcgttt atcccttcgg tctccaggga cagtggaatg acggccgcgg caagtcgttc
     9361 cgccggcggg acaattccag cgtggatgat gtcggtttca tcggcgcggt catcgacgag
     9421 ttgttccggg agttctcgat tgaccgccgg cgggtttacg tcatgggggt ctccaacggc
     9481 ggcatgatga ccctgcgcct gggcatcgaa ctcggagacc gggtcgccgc catcgcggcc
     9541 gtggttgcca accttcccga ccggctcgct tccgcccgcc cggtccgtcc cctgccggtt
     9601 cttatcatga acggcacccg ggatccgctg gtcccctgga agggtggggc ggtgcgggtc
     9661 ttcggcaagg attacggcga tgttctttcg accgaatcaa cggtcgcctt ctgggtcaag
     9721 gccaacgggc tgaacggtcc ggttgaaaag aatcagcttc cggaccgggt ctccacggac
     9781 gggtgccgcg tggaggtctc cgcctaccgc tcggaagggg atcggccccc ggtcattctt
     9841 tacgccatcc agggcggcgg ccacagtttt cccggggccc ggaccttgct gcgctatatc
     9901 ctgctgggaa acaagtgcat ggatatcaac ggcgcccagg tgatctggga cttctgcaag
     9961 gattattcgc ttcaaggcga atgatccttt gcctggatcg tggccgccgt tcattttttc
    10021 gcggtttccc cggtccggcc ggccggggcc ggaccgccga tcagccatga aaaagagaat
    10081 cacgcttctg atcttagttc tgctggtcgt cccggccatt ctggccggaa ttattcaaat
    10141 tctggccaat ccgctggcca aaaaggcggt tgaggccagg gtcggaccgt tattcgaagg
    10201 acggctgagc atcggatcgg tgcggatttc ggtcccgggc cggtcgatta tcctcaggaa
    10261 cctgctgctc cgccagccgc cgggcttccc gccgggcaac ctgctgacgg ttgacgagat
    10321 ccgggtgcaa gcggctttcg cgccgctgct ccggcggcgc ctggtcatca accgcctgct
    10381 tatttccaac ccggaaatca atatcatcca ggcggccggc ggcgccacca acctggaaca
    10441 tgatctcggc cgattcgcag tcaaaaaggg tgggaaagaa cctcccgcct tcaagttcca
    10501 ccttgatggt ctctcaattc ggggcgggcg ggttaacctt tacagccggc agttgagccc
    10561 ggcgaaggag ccggccctga gtgtcagccg tctcggcctg actctcggca acttgaactt
    10621 tcccaacgag gagcgtctgg agagtccttt caaaacgcag gcggtgatcg agacggtcaa
    10681 cccggttccg gtggccgtcc gcggccggat cgtggccggc gccggtccgc tcgatttcga
    10741 cgcctcgacc gaaatccggg gcgtcaacct ggctgatttc gcttatcttt atcccgccgc
    10801 cccgatttca atcacggccg gccgggctga cgtccgggcc gaagcccgct gtcgccaaaa
    10861 cgatctggaa agccggcagc acctggaaat ccggggtttg aaactggccg gctcgggtgg
    10921 ttttttcaag aagaccctct tgggcctgcc ggcggcggcc gcggtcaagt tcctggagga
    10981 ccgggagggt gccctctccc tggatttcga agtcaagggt acggtcaacg aattgaagag
    11041 cgatctgaaa gaggcgattg ccgcctcctt cagccgcgcc ttccgggaga agttcggccg
    11101 gccgattctg gccgtggacc ggttgatcgg cggagcggcc cgggggctgt ggaccgggat
    11161 cgggggcggt cttagaaagg ccggaaaagg catctcgaac ctgatgggcc ggtgaccggc
    11221 caaaccagat cgctgaagga gagacggaat gatcatcagg aaactctgcc tggccgtagc
    11281 cggttccttt ccttttttct tgtgcttttc ggtttggccg gtgccggcgt tcgggcagga
    11341 aatgcgtccc gccatcaaaa acccctatta cgagaagccg gcctggaaac tctggtgcga
    11401 tgacccggct ccggtcgggg acgtctccct gccgcccgag gcgcagcgcc tcttcccctg
    11461 gccgcccgct tccgagcggt tcgttgatta ccgcaagggt caggattggc cgggccgcac
    11521 ctacgccggt ttctggtgtg acgtctattc gagcgaggat aacaagcaca gttacgtccg
    11581 ggacgcttgg aaatcgccgg ccggcgataa tgcggtcgag ggcatcctgg agggcatgat
    11641 ccatctcaac cggggtgaga tcgggtcgca cggaatcaat ttcaccagcc agtcgacgac
    11701 cgagatgggc caccatatcg ccaacgaaaa agatcgcctg gagcggaccg aacgggacta
    11761 ctattttcgg gactgcctcc ggaccgggcc ggcccatatc tccttcatgg accgggctcc
    11821 ggatcgggcc gtggacgagt accaggccct ggcgccctgc ttctacaatt ccatcgggtc
    11881 ctcgggcagc gagacgatgg ccatcaccaa gatgctgatc gccggtggct acctgccggt
    11941 cgccctgaag cgcaccctcc aggtgaacgg cctttacccg gccacgct
//
LOCUS       DAOQYN010000028        11959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_5832_length_11959_cov_4.074009, whole genome shotgun sequence.
ACCESSION   DAOQYN010000028 DAOQYN010000000
VERSION     DAOQYN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11959
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5832_length_11959_cov_4.074009"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3010)
                     /locus_tag="PKN80_02165"
     CDS             complement(<1..3010)
                     /locus_tag="PKN80_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34849.1"
                     /translation="MQHGFVFYNSTTNGKPNYGGKPMNLRRLLSIAFLTLSLALSASP
                     ALATISGSSADYDSTNFDVLAGQRRAALSLSLTSDNDGDSFNNVTVHINNVSNLTAGE
                     ISKIAIYEDTNNNSFFDVLTDNLLLDTVPVIGTDQVLDFADISVSTWQGSVNNRNKTY
                     FIVVDTSYSLDVLDSFSVYHAAIGSLGANTSSNLTCNFSFLAPNAPAQGVSNPNQVNE
                     YFRIRWNNQTDTQINLYYVPSTAYVPNDPLSGATLIVSGLASTEVSYDWNTYSLHSGE
                     YYIIATLGDGGGVGTASSVLASVRIAPRRFSGVVSDIAKDTVASGLFTEDGVFLLKLK
                     TEVATDTLQTVQVRIVNVDNTIASNEIMTVSLHRDRYTGSYDNDKYDEGRDYWVPPTG
                     TEVSAPVGTPVNININNYPTASKEGDVPQRLASGPLTSGWYYQNGTYYCDYLIVVRAA
                     VNLDDGDKFEVELLGSTALIKDGAVEQPYSVNLSGTFISKEITCENLSDFYPNINYVN
                     PARHFESDQNITLRWKNNTGLLVNLYYRNVDGFNRAAPLSGAVRFASDQGSGPYYWRC
                     DGVPAGRYYIIIEFIPPSSGGLAPAPLVFPGTVEFVQPAVTAENLVSKDQGILSRSGP
                     TAVFGLKIAAPPSGLTSPGFNKIQHLIRLTVKADLSSPDIDIRPYQVAIYKNDGEYLE
                     AFDPGDEQVTILPFPDVVDEIVFELGETCPVPNDVPAHNEYFVVVYSRPEMRNNQQIK
                     LILKEVVIGDVNPEYNPGMIDDDVYLYDPKYKPEDRKNAILGSPESESLTCEQRIVDL
                     RPMRSDQWSHGVMKGGQPFYLNDYPSQNQSGANTYIYSPETFQWWHGDDHPAPFGPEE
                     RELMGMYDPIAVLGLDLAATGGFTDAVHGTEKLVKLAVIIHDGWPENFNPNEALATHF
                     LSNWPNISLVRDEDGNGHFNPAIDTAQFYDPANKNITFSRVSSQGLWQASHTYTRGQI
                     IQWRTPISGVTWTTGATYVLGARVVPTVPNG"
     gene            3397..3969
                     /locus_tag="PKN80_02170"
     CDS             3397..3969
                     /locus_tag="PKN80_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNQ34850.1"
                     /translation="MARKKRILLGVTGSIAAFKAAALASKLVQADCQVRVAMTPEAVK
                     FVTPLTFQSLTGERVYLDMFDEAGAAEWEIEHVSLAEWADLVLVCPATANLISKAAVG
                     LADDLLSATLLTTRKPVVFVPAMNSGMWNNPILQARVAELKRHGHAFLGPAAGRLACG
                     TSGTGRMVEVEAVLKFVLQMKTHQNREKKC"
     gene            3963..4730
                     /locus_tag="PKN80_02175"
     CDS             3963..4730
                     /locus_tag="PKN80_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ34851.1"
                     /translation="MLKVGAFLGSFRLGAKESFDKCRELGLDGIELSNIRDELMIESL
                     SESAIEDIRHRAAAAGLVISSVCGDLGGHEFTDSGDVEERIKRTMKIMDVTRALGSRI
                     VQTHIGSIPADPEAPARKVMAYAMDRLGEYGDRIECRLASETGPEAAEELGAFLKEIK
                     HESIKINYDPANLVMKGFDHLAGVAILKDYIVQTHAKDGKRRNGEVPLGEGDVDFPVY
                     IKALRDIGFDGFFIIEREVGPNPAEDIARAKDFLRRF"
     gene            4893..6272
                     /locus_tag="PKN80_02180"
     CDS             4893..6272
                     /locus_tag="PKN80_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNQ34852.1"
                     /translation="MGLKEFSLVPKDYPKVETPYRRIKTALPVPESIDFFKKLWASEP
                     ISMRCEPPVLWHRSSGLNVFDPYGNKWLDWSCGVLVTSVGHNHPAIRAAIEKHLQSGA
                     PLHTYIFANEARARLAEKIIEITPAEHDRVFLLTTGSEAVEVSLKVARTYGVRRNPKK
                     RYVISYQGAFHGRTYASQLAGGGLGGGEWVDPNPTFVQVPYPGSIDTKDKSFGLFEKS
                     LAAAGIKPEEVAAVIAETFQGREAKLMPVDYVKNLRQWCDRYDAALIFDEVQAGFGRT
                     GRLFGFEHYGVVPDLFVCGKGISSSLPLSAVIGKSKYLDLYKPGEMTSTHTGSPIPCA
                     AALASIETLQSEKLVENCAAMGAYLKGELEKMVKPYADRLDIGGVGLVHGLLFFNNRQ
                     ELTPDPETAFRFCEEAVGSGNLFFAPVGKGRGAIKFCPPLSVTREAIDDGLYGKRGLA
                     EILKEVMEG"
     gene            6309..7526
                     /locus_tag="PKN80_02185"
     CDS             6309..7526
                     /locus_tag="PKN80_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HNQ34853.1"
                     /translation="MRELKAFLESRRDEAAGLLAELIRFKSVAGEGEDEIQRFLAEKF
                     RALGLEVSLELVPESIKEHPSYTRADRELDYSRRPNLFAAWKGSGPKSVILCAHSDVV
                     PAGDWTEAFNPVIQNGRVIGRGAADDKGHIVSIYYALLALKELGRQPRKSVEVQVVIE
                     EETGGNGALAAILTGHRADGVIVMESTDLSICPANRGAVWYKIEMQGVPVHMGRIREG
                     VNAVEKMAEVIPVLRQYENKLIAESNHPLFREYEQPVQVNIGMIKGGDWPAMVAGWAE
                     MEGGVGFLPTKSLEDVQKELTALLQNHPDPWVREKSKVSFPKLHNDAYETRLDDPLVT
                     GLKESAVAAGASGKVTGLIVSCDARLYAKVGQMPTVVFGPDSIKQAHSREESLSIDEM
                     VKASEIIARYLLA"
     gene            7622..8695
                     /locus_tag="PKN80_02190"
     CDS             7622..8695
                     /locus_tag="PKN80_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34854.1"
                     /translation="MLKIGVIGGGKFGTNHLIAFSQLEKSGRAKLVALADLSPEILKT
                     QQARFGVKTYSDHREMLAREELDGVTVATPDHAHRSVTLDALAAGKPVLVEKPLDTTV
                     EGCQEIIAAARQNKCFLQVDFHKRYDPYHQETERLVREGNLGEIQYGYVHMEDRIEVP
                     RDWFPGWAPKSSPAWFLGIHFYDLIRWIIKKEPVEVYATGTKRVLVKLGVDTYDSIQA
                     KISFEGGASVSFDTSWITPDGFEAVVNQGFRLMGDRGVAEVDSQDRGMRTCLVGAGMQ
                     THNLGFMVEKRDKWGKNFLAGYGIEAIADFVDNLEYLKSGGDPAALAGTITALGEDGL
                     AATRMAAAVHESIKSGRPVNLKG"
     gene            8730..9509
                     /gene="proB"
                     /locus_tag="PKN80_02195"
     CDS             8730..9509
                     /gene="proB"
                     /locus_tag="PKN80_02195"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNQ34855.1"
                     /translation="MKDRRAIMKRVKRLVVKVGTSILTNRKNRLDASYLEELVEDIAG
                     LRNRGLEVSLVTSGAIAAGLKSLKLASRPRDLASLQAAAAIGQSNLMRTYERLFKEQG
                     YLVGQVLLTRDVVNNRDRFGNARNTLDRLLSCGVVPIINENDAVAVEEIRFGDNDTLS
                     ALVLKLTAADLLIILSDVDGLQVEGRLCPEVARIDARISRAAQGSQSDKTVGGMTTKL
                     EAARLVTAEGKPMIIANGREERILNRILDGEKLGTFFRPAG"
     gene            9558..10154
                     /locus_tag="PKN80_02200"
     CDS             9558..10154
                     /locus_tag="PKN80_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNQ34856.1"
                     /translation="MFYKFLKSLLNIFCLLFFRLEVRGVENIPRRGGAILASNHLSLL
                     DPPLVALRIPRRIRFLSKTVLYRNSLLEPIIKSLGIIPLRQHSGAVGLKSVIRSLKSG
                     HAVLIFPEGSRNPNGSELLPGQPGITLAVRATGVPVVPVLISGTEKALPLNAVIFRPR
                     KVLIRYGPPLLYRGEADFADRVMAAIDRLRPDNPERPE"
     gene            10159..11019
                     /gene="ispH"
                     /locus_tag="PKN80_02205"
     CDS             10159..11019
                     /gene="ispH"
                     /locus_tag="PKN80_02205"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNQ34857.1"
                     /translation="MARVLTAREIGFCFGVKRAVSLCEETLRRHGACFSIGPVIHNER
                     VMENLTARGLKIVREMPDGRTPALVRSHGLHPEKLRALSKSGREVVDATCPFVSRVQK
                     LVTRLRRQGYRVLIIGEPDHPEVKALAGFAGNSALICPAGRSAARRWAAVNFRTPPAK
                     RVAVVSQTTSTEPVYRETLAALLKAGLPGETRIFDTVCRVTEKRQAEALELAGKTDLL
                     LVVGSRKSANTRNLYRLVRKRKPAAYFISGPEEVRDDWCRPNLTVGLVSGTSTPVETI
                     KEIKKKIERT"
     gene            11040..>11959
                     /locus_tag="PKN80_02210"
     CDS             11040..>11959
                     /locus_tag="PKN80_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNQ34858.1"
                     /translation="MTSWMTKQMTAFKPGQIITGKVIEVANDYVLIDIGYKSEGYVPR
                     MEFKDFETLESGQEVRVMLETLDINENGFISLSKEKADLLLNWDRVETSYRQGETIKG
                     KILASVKGGFRVDIGIMAFLPISQTDIRPISDPKALIGQVFDFKVIKLDRSRNNIVIS
                     RRELLEAEERIRKTAALARLEIGQVVKGLVKNIVDYGAFIDIGDLTGLAHINDLSWSH
                     ITHPSQVLAIGDRVEVKILKINQEKGEVSLGLKQTMKNPWEGIESRYPVGSRIQGKVV
                     NITDYGAFVKLEDGVEGLLHISELSWTNKV"
BASE COUNT         2684 a         3526 c         3470 g         2279 t
ORIGIN      
        1 ggccgttggg cacggtgggc accacccgcg cgcctaaaac ataggtggcg ccggtggtcc
       61 aggttacgcc ggagatcggc gtccgccatt gaatgatctg accccgggta taggtgtggc
      121 tcgcctgcca gagcccctgg gaggaaaccc gggaaaaagt aatattcttg ttggccgggt
      181 cgtaaaactg tgcggtatca atcgccgggt tgaagtggcc gttgccgtcc tcgtcccgga
      241 ccagggagat attcggccaa ttggacaaga agtgggtggc cagcgcttcg ttgggattga
      301 aattctccgg ccagccgtcg tggatgatca ccgccagctt gaccaatttt tcggtcccgt
      361 ggaccgcgtc ggtgaaaccg ccggtggcgg ccagatccag gcccaggacc gcgatcggat
      421 cgtacatgcc catcaattcc cgttcctccg gcccgaaagg cgccggatgg tcgtccccgt
      481 gccaccactg gaaggtctcc gggctatata tataggtgtt ggccccgctc tggttctggc
      541 tgggataatc gttcagatag aagggttggc cacccttcat caccccgtgt gaccattggt
      601 cactgcgcat cggacgcagg tcaacgatcc gctgttcgca ggtgagactt tccgattccg
      661 gggagcccaa aattgcattc ttacgatcct caggcttgta ttttggatca tacaagtaaa
      721 catcatcgtc aatcatccct ggattatatt ccggatttac atcgccaata actacttcct
      781 ttaaaattaa ttttatctgc tggttgtttc gcatctccgg ccggctgtaa accacgacaa
      841 aatattcatt atgagcagga acgtcgttgg gaaccggaca ggtctccccc agttcaaaaa
      901 caatctcgtc tactacatcg ggaaatggaa gaatggttac ttgttcgtct cccggatcaa
      961 aggcttcgag atattccccg tcatttttgt aaattgcaac ttgatacgga cgaatatcaa
     1021 tatctggact cgaaagatcg gccttgactg ttaatctaat taaatgctgt atcttgttaa
     1081 atccagggct ggtcaaacct gaaggcggtg cggcaatttt caggccgaag acggccgtcg
     1141 ggccgctgcg cgacaggata ccctgatcct tgctaaccag gttttccgca gttactgcgg
     1201 gctgcacaaa ctccaccgtg cctggaaaaa cgagtggtgc cggagcaaga ccaccggaag
     1261 atggcggaat gaactctata attatgtaat aacggccggc cggaactccg tcacaacgcc
     1321 agtaataagg accgctgcct tgatctgagg caaaccttac cgctccggat aacggtgcgg
     1381 cacgattaaa accatcaaca ttgcgatagt aaagatttac caacagaccg gtgttattct
     1441 tccagcgcag ggtaatgttt tgatctgatt caaagtggcg ggctggattg acataattta
     1501 tattcggata aaaatcggat agattttcgc aggtaatctc ttttgaaata aaagtaccac
     1561 tcaaattcac tgaatatggt tgttccacag cgccatcttt aataagagcc gtgctcccca
     1621 acaattccac ttcaaattta tccccatcat caaggtttac agcggccctg accacaatca
     1681 agtaatcgca gtaataagtg ccgttctggt agtaccagcc ggaggtaagc ggaccgctcg
     1741 ccaatcgttg gggcacatca ccttccttcg aagcggtcgg ataattgttt atgtttatat
     1801 tgaccggcgt tcccactggg gctgaaacct cggtgcctgt agggggaacc caataatcac
     1861 gcccttcatc gtacttgtcg ttgtcataag aaccggtgta acggtcgcga tggagggaaa
     1921 cagtcattat ttcgttgctg gcaatcgtgt tgtcaacatt gacaatcctc acctggactg
     1981 tctgaagggt gtcggtggcc acctcggtct tcagcttcaa cagaaaaacg ccatcttcgg
     2041 taaataaacc cgacgccacc gtatccttcg caatgtcgga aacaaccccc gaaaaacggc
     2101 gcggggcgat ccggaccgag gcaagcacgc tggaagcggt cccaactcca ccgccatcgc
     2161 ccaaggtggc gattatgtag tattcgccgg aatgcaggct ataggtgttc cagtcatagg
     2221 aaacctcagt cgaggccagg cccgaaacga tcagggtggc gccgcttaat ggatcattgg
     2281 gcacgtaagc ggtgctcggc acataataaa ggttaatctg ggtgtcggtc tggttattcc
     2341 agcgaatccg gaagtactcg ttaacctgat tcggattgct gacaccctgg gccggtgcgt
     2401 tcggggcaag gaaagaaaaa ttacaggtga gattactgct ggtatttgca cctaaggatc
     2461 cgattgctgc atgatagacg ctaaaagaat caagcacatc caaagaatag ctggtatcta
     2521 caacaataaa atacgtctta ttcctattat taacgcttcc ctgccaagtt gaaacactta
     2581 tgtcggcaaa atcaagcacc tgatcagttc ctatcacagg aactgtatcc aaaagaagat
     2641 tatccgttaa aacatcaaaa aaactgttat tgttagtatc ttcgtaaatt gcaatttttg
     2701 aaatttctcc ggctgttaaa tttgaaacat tgtttatatg aaccgtaaca ttattaaaag
     2761 aatctccgtc attatcagaa gttaaagata aagataacgc ggctctcctt tggccagcca
     2821 aaacatcaaa attagttgag tcgtaatcag cagaactccc actaatagtg gctaaggccg
     2881 gggaggcaga aagggcaaga ctgagggtta agaaggcgat gctcaggaga cgccgcaaat
     2941 tcatcggttt acctccgtag ttgggtttgc cgttagtcgt cgagttgtag aagacgaagc
     3001 catgctgcat ataatccttg ctgtattatt taatttcata aaaaagaatc aaggtatcag
     3061 catattaaac cctaactccg ctggtactgt caaattaccg cccttcccca gggaaaaggc
     3121 ggaggcgggt aaaattcata agttttgctc cgccaaatcc ccctcttttc cgaatccatt
     3181 gggagtctcg aatctttatg tatgttagta ccgaaaaaag aaacggggtt cacttcgctt
     3241 cccgccaaac aaagcccgaa ttttctgctt gccttcttat aaacacttta gattaaaccg
     3301 gaagtctctt tgtttagctc tttgctaatc attcagggcc atccgcacct ttaaagcatc
     3361 ttgtttttat caaggataat atgttaaaat tcagcgatgg ccagaaaaaa aagaatcctg
     3421 ctcggggtca ccggcagcat cgccgccttc aaggcggccg cgctggcctc caagctggtc
     3481 caggccgatt gccaggtccg ggtggcgatg acccccgagg cggtcaagtt cgtcaccccc
     3541 ctcaccttcc agagtctaac cggagagcgg gtttatcttg acatgttcga tgaggccggt
     3601 gcggccgagt gggagattga acacgtttcg ctggccgagt gggccgacct cgtcctggta
     3661 tgcccggcca cggccaacct gatcagcaag gcggccgtcg ggctggccga tgacctgctc
     3721 tcggccaccc tgctgaccac ccggaagccg gtcgtcttcg taccggccat gaactccggc
     3781 atgtggaaca accccattct gcaggcccgg gtggccgaac tgaagcgtca tggccatgct
     3841 ttcctgggac cggccgccgg ccggctggcc tgcggcacct ccggaacggg ccggatggtt
     3901 gaggtggaag cggtcctcaa gtttgtcctt cagatgaaga cgcaccaaaa ccgggagaaa
     3961 aaatgctgaa agtcggggct tttctgggat cattccgcct gggggcaaag gaatcctttg
     4021 acaagtgccg ggaactcggc ctggatggca tcgagttgag caatatccgg gacgaactga
     4081 tgatcgaatc cctctccgag agcgcgatcg aagatatccg ccaccgggcc gccgcggccg
     4141 ggctggtcat cagttcggtc tgcggagacc tgggcggcca cgagttcacc gactccggcg
     4201 acgttgagga acggatcaag cgcaccatga agatcatgga tgtcacccgg gccctcggtt
     4261 cccggatcgt ccagacccac atcggctcca ttccggccga cccggaggcg ccggcccgca
     4321 aggtcatggc ttatgccatg gaccggctgg gcgagtacgg agaccggatc gagtgccggc
     4381 tggcctcgga aaccggcccc gaggcggccg aagaactggg tgcttttctc aaggagatca
     4441 agcacgaatc gatcaagatc aactatgacc cggccaacct ggtgatgaag ggcttcgacc
     4501 acctggccgg ggtcgccatt ctcaaggatt acattgtcca gacccacgcc aaggacggca
     4561 agcgccggaa cggcgaagtt ccgctgggcg agggagacgt tgattttccg gtttatatca
     4621 aggccctccg ggatatcggc tttgacggtt tcttcattat cgaacgggaa gtcggtccga
     4681 acccggccga agacattgcc cgggccaagg acttcctgcg ccgtttttaa ggattcccgc
     4741 cctgtttcca gggctgccgg cgaaacccgg cctttgtaag aaccggagcc cggaaagaat
     4801 ttgataaacc gaggggcctc cgggcgtgat tttttcgaaa aacaacccgg cgtgaaagcc
     4861 ggcacaccca catcaggaaa ggagaaggtc aaatgggatt gaaggaattc tctctggtgc
     4921 cgaaggatta tccgaaggtt gaaactccct accggcgcat caagaccgcc ctcccggtcc
     4981 ccgaatcgat cgatttcttc aagaaactct gggccagcga accgatttcc atgcgctgcg
     5041 agccgccggt gctctggcac cggagcagcg gtctgaacgt cttcgatcct tacgggaaca
     5101 agtggctgga ctggtcctgc ggggtgctgg taaccagcgt cggccacaat cacccggcca
     5161 tccgggcggc cattgaaaag caccttcaga gcggcgcgcc cctgcatact tacatctttg
     5221 ccaacgaggc ccgggcccgg cttgcggaaa agatcatcga aatcaccccg gccgagcatg
     5281 accgggtctt cctgctgacc accggttccg aggcggtcga ggtctccctg aaggtggccc
     5341 ggacctacgg cgtccgccgc aacccgaaga agcgctacgt cataagttac cagggggcct
     5401 tccacggacg cacctatgcc tcccagctgg ccggcggcgg cctgggcggc ggcgagtggg
     5461 tcgaccccaa cccgaccttt gtccaggtgc cctacccggg cagcatcgat accaaggaca
     5521 agagtttcgg actctttgaa aagagcctgg ccgcggccgg catcaagccg gaagaggtgg
     5581 cggcggtcat cgccgaaacc ttccaggggc gcgaggccaa gctgatgccg gtcgattacg
     5641 tcaaaaacct gcgccagtgg tgcgaccggt atgacgcggc gctcatcttt gacgaggtcc
     5701 aggccgggtt cggccggacc ggccgcctct tcggcttcga acactacggg gtcgtgcccg
     5761 acctcttcgt ctgcggcaag ggaatttcca gttcactgcc cctctcggcg gtgatcggca
     5821 aatcgaagta cctcgacctt tacaaaccgg gcgagatgac cagcacccac accggcagcc
     5881 cgataccctg cgcggcggcc ctggccagca tcgaaacgct ccagtccgaa aagctggtcg
     5941 aaaactgcgc ggcgatgggc gcctacctca agggcgaact ggaaaagatg gtgaagccgt
     6001 acgccgaccg gcttgatatc ggcggggtcg gcctggtcca cggcctgctc ttcttcaaca
     6061 accgccagga actgactccg gacccggaga ccgccttccg tttctgcgaa gaggcggtcg
     6121 gctccggcaa cctcttcttc gcgccggtcg gcaaggggcg cggcgccatc aagttctgcc
     6181 cgccgctttc ggtgacccgg gaggccatcg acgacggcct ctatggcaaa cggggcctgg
     6241 ccgaaattct gaaagaggtc atggagggat gactttacct taacgagcac gccaaccgcg
     6301 ggagaaacat gcgcgaattg aaagcgttcc ttgaaagccg ccgggacgag gcggccggcc
     6361 tgctggccga actgatccgt ttcaagagcg tggccggcga gggcgaagat gaaatccagc
     6421 gtttcctggc cgaaaaattc cgggccctcg gcctggaagt cagcctggaa ctggttcccg
     6481 agagcatcaa ggagcacccc tcctataccc gggccgaccg cgaactcgat tacagccggc
     6541 ggcccaacct tttcgccgcc tggaagggaa gcggaccgaa atcggtcatc ctctgcgccc
     6601 acagcgacgt ggtgccggcc ggggactgga ccgaggcctt caacccggtg atccaaaacg
     6661 ggcgggtcat cggccggggc gccgccgacg acaagggaca cattgtcagc atttactacg
     6721 ccctgctggc cctcaaggaa ctcggacgcc agcccagaaa gagcgtcgag gtccaggtgg
     6781 tcatcgagga ggagaccggc ggcaacgggg ccctggccgc catcctgacc ggccaccggg
     6841 ccgacggcgt catcgtcatg gaatccaccg acctcagcat ctgcccggcc aaccgggggg
     6901 cggtctggta caagatcgaa atgcaggggg tgccggtcca catgggacgc atccgcgaag
     6961 gcgtcaacgc cgtggaaaaa atggccgagg tgatacccgt cctgcgccag tacgagaaca
     7021 agctgatagc cgagagcaac cacccgctct tccgcgagta cgaacagccg gtccaggtca
     7081 atatcggcat gatcaagggc ggcgactggc cggccatggt ggccggctgg gccgaaatgg
     7141 agggcggtgt cggtttcctg cccaccaagt cgctagagga cgtgcagaag gagctgaccg
     7201 cgctcctgca gaaccatccc gatccctggg tgcgggagaa gagcaaggtc tccttcccga
     7261 aactccacaa cgacgcctac gaaacccggc tcgacgaccc gctggtgacc ggactgaagg
     7321 agagcgcggt cgccgccggc gcctccggca aggtcaccgg cctgatcgtc agctgcgacg
     7381 cccgcctcta tgccaaggtc ggccagatgc ccacggtggt cttcggaccc gacagcatca
     7441 agcaggcgca cagccgggag gagagcctct ccatcgacga aatggtcaag gcctcggaaa
     7501 tcatcgcccg ctacctgctg gcctgaaaat tctgaaacag aaaccggcga cccggccgga
     7561 acggaaaccg ggattcaagc ttcaagaagt tttgagattc caataaaacg ggaggaaaaa
     7621 aatgctgaag atcggtgtca tcggaggcgg caagttcgga accaaccacc tgatcgcctt
     7681 ctcccagctg gaaaaatcgg gccgggccaa gctggtcgcc ctggccgatc tcagcccgga
     7741 gatcctcaag acccagcagg cccgcttcgg cgtcaagacc tacagcgacc accgcgaaat
     7801 gcttgcccgc gaagaactgg acggcgttac ggtggccacg ccggaccatg cccaccggtc
     7861 agtgacgctc gacgccctgg ccgccggcaa gccggtcctg gtcgagaagc cgctggacac
     7921 caccgtggag ggctgccagg agatcatcgc cgcggcccgc cagaacaagt gcttcctcca
     7981 ggttgacttc cacaagcggt acgaccccta ccaccaggag accgaacggc tggtccgcga
     8041 aggcaatctg ggcgaaatcc agtacggtta cgtccacatg gaagaccgga tcgaggttcc
     8101 gcgcgactgg ttccccggct gggcgcccaa gtcctcaccg gcctggtttc tcggcatcca
     8161 cttctacgac ctcatccgct ggatcatcaa gaaggagccg gtcgaggtct acgccaccgg
     8221 caccaagcgg gtcctggtca agctgggcgt cgacacctac gactccatcc aggccaagat
     8281 cagtttcgag ggcggcgcct cggtcagttt tgacacctcc tggatcaccc cggacgggtt
     8341 cgaggcggtg gttaaccagg gcttccggct gatgggagac cggggcgtgg ccgaggtgga
     8401 cagccaggac cggggcatgc ggacctgcct ggtcggagcc gggatgcaaa cccacaacct
     8461 gggcttcatg gtcgaaaaac gggacaagtg gggcaaaaac ttcctggccg gctacggaat
     8521 cgaggccatc gccgatttcg ttgacaacct cgagtacctc aagtccggcg gcgatccggc
     8581 tgccctggcc ggcaccatca ccgccctggg cgaggacggg ctggcggcca cccggatggc
     8641 ggccgcggtc catgaaagca tcaagagcgg ccggccggtc aacctgaagg gctgagtccc
     8701 cggcctcccc tgaaaaacaa ggcggatcaa tgaaagaccg gcgcgccatc atgaaacggg
     8761 tcaaacggct ggtggtcaag gtcgggacca gtatcctgac caaccgcaag aaccgcctgg
     8821 acgcctccta cctggaggag ctggtcgaag atatcgccgg cctgcgtaat cgcggcctgg
     8881 aagtttcgct ggtcacctcg ggggccatcg ccgccggcct gaaatcgctg aaactggcca
     8941 gccgcccccg ggacctggcc agcctccagg cggccgcggc catcggccag agcaatctca
     9001 tgcgcaccta cgagcgcctc ttcaaggagc agggttacct ggtgggccag gtcctgctga
     9061 cccgggatgt cgtaaacaac cgggaccggt tcggcaacgc ccgcaacacc ctggaccggt
     9121 tgctctcctg cggcgtggtg ccgatcatca acgaaaacga cgcggtggcg gtcgaagaga
     9181 tccgcttcgg cgacaacgac acgctctcgg ccctggtgct aaagcttacc gccgccgacc
     9241 tgctgataat actttcggac gttgacggcc tgcaggtcga aggccgcctc tgccctgaag
     9301 tggcgcggat cgacgcccgg atctcccggg ccgcccaggg cagccaatcc gacaagaccg
     9361 tgggcggcat gaccaccaag ctggaggcgg cccggctcgt gaccgccgag ggcaaaccca
     9421 tgatcatcgc caacggccgc gaagaaagaa tcctgaaccg aatcctggac ggcgaaaaac
     9481 tgggcacttt tttccggccg gccggttgat tttggttgac attcttatct caaaaagatt
     9541 aaaattacaa tttcggaatg ttttataaat ttctgaaatc gcttttaaac attttctgtc
     9601 ttctcttctt caggctggaa gtacggggag tggagaacat ccccaggcgg ggcggcgcga
     9661 tactggcctc caaccacctg agtctcctgg atccgcccct ggtggcgctc cggatccccc
     9721 ggcggatacg ctttctttcc aagaccgttc tgtaccggaa cagcctgctg gagccgatca
     9781 taaagagcct gggcattata ccgctgcgcc agcacagcgg cgcggtggga ttgaaaagcg
     9841 tcatccgttc cttgaaatcc gggcatgcgg tgctgatctt cccggaaggg tcccgcaacc
     9901 cgaacggctc ggaactcctg cccgggcaac cgggtataac tctggccgtc cgggctaccg
     9961 gggttccggt ggttccggtc ctgatcagcg gaaccgaaaa ggccttgccg ctcaacgccg
    10021 tcatctttcg gccgcgaaaa gtattaatcc gttatggacc gcccctcctc taccgggggg
    10081 aagcggactt tgccgaccgg gtcatggcgg ccatcgaccg cctgcgtccg gacaatccgg
    10141 agcggccgga ataagctgat ggcccgggta ctgaccgcgc gggaaatagg tttttgcttc
    10201 ggcgtcaagc gggcggtttc cctttgcgaa gaaaccttgc gccgtcacgg ggcctgtttt
    10261 tcgatcgggc cggtcatcca taacgaacgg gttatggaaa acctgaccgc ccgcggcctg
    10321 aaaattgtgc gcgagatgcc ggacggccgg actcccgcgc tggtacgcag ccacgggctg
    10381 cacccggaaa aactgcgcgc gctttcgaaa tcaggacggg aggtggtcga cgccacctgc
    10441 ccgttcgtga gccgggtcca gaagctggtc acccggctgc gccgccaggg ttaccgggtg
    10501 ctgatcatcg gcgagccgga ccacccggaa gtcaaggccc tggccggttt cgcgggaaat
    10561 tcagctctga tctgcccggc gggccgatcg gcggcccggc gctgggcggc cgtcaatttc
    10621 aggactccgc cggcgaaacg ggtggcggtg gtcagccaga ccaccagcac cgaaccggtt
    10681 taccgggaaa cgctggcggc cctgctgaaa gccgggctac ccggtgagac ccgcatcttt
    10741 gataccgtct gccgggtgac cgagaaacgg caggccgagg cgctggagct ggcgggaaaa
    10801 accgacctgc tgctggtggt cggttccagg aaaagcgcca atacgcggaa tctctaccgg
    10861 ctggtgcgca agcgaaagcc ggctgcttac tttatttccg gcccggagga agtcagggac
    10921 gactggtgcc ggccgaacct tacggtcggc ctggtctccg gaacctccac gccggttgaa
    10981 actataaaag aaatcaagaa aaaaatcgaa aggacgtgac tctgatgcaa gaagaaaaaa
    11041 tgacgagttg gatgaccaag cagatgacgg cgttcaaacc cggtcagatt atcaccggga
    11101 aagtaatcga ggtggccaac gattacgtgt tgatcgacat cggttacaag tccgaagggt
    11161 acgtgccccg gatggaattc aaggatttcg agacgctgga gtcgggccag gaagtccggg
    11221 tgatgctgga aaccctggac atcaacgaaa acggcttcat ctccctttcc aaggagaagg
    11281 ccgacctcct gctgaactgg gaccgggtcg agacctccta ccgacagggc gaaaccatca
    11341 agggcaagat cctggcctcg gtcaagggcg gcttccgggt ggacatcggc atcatggcct
    11401 tcctgcccat cagccagacc gacatccggc ccatctccga ccccaaggcc ctgatcggcc
    11461 aggtcttcga tttcaaggtc atcaaactgg accgttcgcg gaacaacatc gtcatttctc
    11521 ggcgtgagct gcttgaagcc gaggaacgaa tccgcaagac cgccgcgctg gcccgcctgg
    11581 aaatcggcca ggtggtgaag ggcctggtca agaacatcgt cgattacggc gccttcatcg
    11641 atatcggcga cctgaccggc ctggcccata tcaacgacct ctcctggagc cacatcaccc
    11701 acccgtcgca ggtgctggcc atcggcgacc gcgtcgaggt caaaatcctg aagatcaacc
    11761 aggaaaaggg cgaggtgtca ctcggactca agcagaccat gaagaacccc tgggagggca
    11821 tcgaaagccg ttacccggtc ggcagccgca tccagggcaa ggtggtcaac atcaccgatt
    11881 acggcgcgtt cgtgaagctc gaggacggcg tggagggact gctccacatc tcggaactct
    11941 cctggaccaa caaggtcaa
//
LOCUS       DAOQYN010000029        11710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_5950_length_11710_cov_4.865551, whole genome shotgun sequence.
ACCESSION   DAOQYN010000029 DAOQYN010000000
VERSION     DAOQYN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11710
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5950_length_11710_cov_4.865551"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3855)
                     /locus_tag="PKN80_02215"
     CDS             complement(<1..3855)
                     /locus_tag="PKN80_02215"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNQ34859.1"
                     /translation="MTGIKPPAIPGYRPFLSKLIAVGLLATLPGLLSLAGPARAESIT
                     GKVTRDGTNGIAGVRVNAYRVDYNLSSPSASALTDAGGNFTLTVAAGTYGLEFDAAAI
                     GYFNEIYKDRYFPGVATRLTVPAGGSFALPENVVLNACGRIGGRVTRDGTNGIAGVQV
                     RVYLEDKVFNSVAETETDTDGNYIAGGLANGNYRVAFSAARLGYYDEWYNDAAGSDSA
                     ELVAVTEGSLRSGVNAVLAGCGSISGTVTDATDGLAGLQVQVYNSSGFLAVQTETGTG
                     GAYRASGLASGNYRVYFSGNSAGYSSSWYNNKSGFDNATSVAVTAPGDTPGINAVLSP
                     AAAILGRVTSGTGIGIPGIQVAAYQQDQAWGYSYTETDDDGYYRLTGLTAGNYQVNFD
                     AEGTGFYGEWYQNAASQAESTPVAVADGAHTEGIDVVLEEMNGGHIYGRVTGPDGRGL
                     AGVQVNLYPAASETSQRWAETDLQGYYALEYLEAGNYKVKANLFNSVYAAQWYSGKYS
                     FEAADAIQVDTATEEGRLKQADFQLAEGGRITGRVKDAFSNIPVAGIQVQVYEADTEF
                     FWGAPWWAVSDDHGNFQVAGLPSGDYLVSFNDWNGAYQSEWYLDRADAASADTVTVTL
                     GAATDLGDVLLNPSASISGTVTDGSRPVAGVQVYYYDASDSQSWDSATTDDAGHFILR
                     NLEPGVYRLWFYGEGAGCFNEFWENRTDLATADTLLVENDSQQVVLPRDVVLARGAAI
                     KGRVTDAATGDPLAGVAVTVHPVSWGGGALNSWNPPQTDSQGNYTVYIVEDGLPDANF
                     KVQFSKNGYFNSWYSGKSSFAEADTVAAPAPGTVENINGSLLRGGRITGRVTDQATGD
                     GLSGVSVTLYDAAGQSIQWSSTDGQGNYSFSPLTSGQYKVGFFLFGYLGEWYDNRTDL
                     SLADTITVTAPETTENIDAALSQKGAIEGTVRDQSSGSPIAGITVHIYDAGQGLVNTD
                     QTDLNGFYQVDNLQTGVYRVYFQTGSSGYLAGWYNGKTDFSSADPVTVTASATATVNG
                     SLIYGSTLSGTVTDVAASPLAAVNVYLYSSLGFQIAFTQTDALGHYSFSGLSAENYRV
                     LFMKDGYIYEWYNDRPAIQDADPVAVIPPAPTNGIDAVLVTPGAIGGTVTDGASPIAG
                     VRVEVYDLNGYLVSAGYTGADGAYLDTGLNAGGYRVHFGGEAAGYFNEWYDDATLDTA
                     AVVNVLPPGTTGNINAVLGRGASISGRVTAADTGQPLAGVQVSIYQDGLLVKTAATAA
                     DGTYTAGALAGGDYQVFFDGRPA"
     gene            4773..6080
                     /locus_tag="PKN80_02220"
     CDS             4773..6080
                     /locus_tag="PKN80_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HNQ34860.1"
                     /translation="MKIETSFRVDALSLLLSLGLLTAARGAGVSVGDLDVTGLNVGDT
                     IELPVLVSGVVPEEFAGFQFEFDFDPAVLEFQGVGHGSLIAMEDAAYAVAPYSPNLWA
                     LEANANASKLLVAGMILMNVDSGHLDLLQLARPASGEGVLLRLRFRLLQKAESLVSVT
                     GTAGRNLLAFDTLQPAASPSTSAPSPLLAGGTDTNGMGNGWELLYLGGIGNDPRGDFD
                     RDGFWNIQEFLGGALPNNNTQAGLGVNLLKSATTGLNLFSIPLADPSIGDTGLARAAD
                     LVSALGGASKVGVIYKWNPATQGWTSHIASLPSINNFDLQAGMGLMVSVNQNISARLL
                     GTPTRFSASLVKNADKTSLNIMVLPAGLTNPPAKAADLVAALGGASKVGVIYKWNPAT
                     QGWTSHIASLPSINNFDLEPGQPLMISVNQNIEWPAAGDWPEL"
     gene            6158..9952
                     /locus_tag="PKN80_02225"
     CDS             6158..9952
                     /locus_tag="PKN80_02225"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34861.1"
                     /translation="MSILKKLGLSIMLVSLLLLGAGAAASFAANPHSIVVTVTNSNSS
                     TPADGALTMNAYIQVRPAEIITLASPSQQYTGGNLLVNLADFSAWAAGEVLVIQLTNT
                     VNQEVGSLNVTITNDDPQLANLQLVAGTGTLIVRSNLQAGSYTITGPAGFNGNLSDGT
                     LSGGYYQKTLSASTSIGSYTVVWGAVAGYFTPANETLNLAKDGSITFTGTYNQIPSYN
                     LTMAVSPAGGGTTVPTTGTSSRLQGTVVNISATPNAGYRWNNWTVNAGVNVDTPASQN
                     TSVTVDVAKTVTANFIKTYTLTVGYTGSGQVSGTTTGVYDVNTPVSLTATPAEHWRFV
                     NWTVNSAVVSTSPTYNTTLDADKGIVALFEPINFVLTVAASPAAGGVVNRDPAGTQVA
                     AGQYQYQEGTQVSLTPVAAVGYQFKEWQGGLTGSNVPGVLTLNANTAVTAVFEQIPYL
                     SINKTEIVFTDTKLTDTLIITNNGQGLLSWAIDVNQNAGSGLKTPMVGWLNVSPLTSG
                     DVPLAAGQSAVVTLTVIQNNSYAELTVRNTTAGINQPDQLVIARINQAPISPDPDGVD
                     FHYLDAAAAPVGEAIDLDTVGVDTPIIITGPSFDDADQNDYQIAREYEIWTTATIPTG
                     VFEPQPFVLDQKVFGESIQGTREWTSFGRNILKPEKTYGLRIRYQDNYSAWSEWTDWI
                     LFKTVAAGETLDDQGQGVPSDQFVDLNTVDLEKYPYLAGHDNDETDAKVVLATEAATG
                     DPVPVLVETNTGELVYLRQDDLGAPTASTTAPYGVFTTRVENIAAGAEVTLTFTFPEA
                     LPADMLWYKYDETTAGWTPYDEANWDFGPAANQVTVRIKDGDYGDADRVENQVAVDPA
                     GPVTLPSGGALGSYSGGDGGCFIATAAFGSYQERHVWMLRLFRDRFLLTNRPGRAFVR
                     FYYRHSPALAGWIAGREWARSAARSLLMPLYGAAYLLVNGLAGMLALAAGLAGLAVLV
                     VRRRRAAVLAAAAACALLLGTAGPAAALDANHLVPAVGESSLVRTYTADLLPAGNWQL
                     GGLFNYVSTPLEGKVNGVEKELSEAQYILTVSGAYALNEKWQLGASFPWLVKQEGELT
                     GVDEAVESAAGDLRLEAKYRFCGGKDQLGYALVPFLTLPTGDDDPARGFFGRPSASAG
                     VIFVADRNWCNHTHVALNLGLQVQEEELDRMTIENTLLFGLGLSRLLSNERTTLALEI
                     NGRADDSWFSEAESTPIEILGSLTQQVNESLRLSFGLGSSLNDGYGAPDYRVLVGLKA
                     AF"
     gene            complement(10033..10836)
                     /locus_tag="PKN80_02230"
     CDS             complement(10033..10836)
                     /locus_tag="PKN80_02230"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ34862.1"
                     /translation="MKFNLGLKKGAGKPAEPKQAELPVPAAEKKAAPPARPPRREKPA
                     RPEKKPKPVVDKEVQHLLKAFTGGKSWTDVETAAGKLLGKGGAAVEPLCEILKGAEKD
                     VLMARNTAYLLGWIGDERAVEPLTHLLKNYNPHIRQAAAESLGMMGSRAAMPALLDML
                     KENEPTLRLAAADALGRIDDPSVKDILAGLLKDDNLDLRWAAARGLARRAAAGRITVK
                     LPENEFGLEKEAETAPPTAVEAAPPEPQQAEETPPAEETKSDDNPFQNY"
     gene            complement(10860..11519)
                     /locus_tag="PKN80_02235"
     CDS             complement(10860..11519)
                     /locus_tag="PKN80_02235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNQ34863.1"
                     /translation="MAASARKFPVGRNSRFLCIGDSITDCGRRKEFPPLGNGYVRHFN
                     TLLQAAFPDLNVQVLNKGIGGNTILDLKQRWEDDVTYHRPDWLSVLIGINDLHHVLRG
                     AADAGQLGPDRFLENYRELLDRATREVGCRMILLEPFYLSTDRTDGWRGRVLKELRPY
                     RQAVARLSREFKAPLVRLQDIFEAQLKERDPETLGTEPVHPNEHGHLIIAWNLFRTLS
                     G"
BASE COUNT         1936 a         3653 c         3903 g         2218 t
ORIGIN      
        1 ggccggccgg ccgtcgaaga agacctggta atcgcctccg gccagggcgc cggcggtata
       61 ggtgccgtcg gcagcggtgg ccgccgtttt aaccaggagc ccgtcctggt aaatggaaac
      121 ctggacgccg gccagcggct ggccggtgtc ggcggcggtc acccggccgg agattgaggc
      181 gccccggccc aggacggcgt tgatgttgcc ggtggttccc ggcgggagca cgttcaccac
      241 ggcggcggtg tcgagggtgg cgtcatcgta ccattcgttg aagtagccgg ccgcctcccc
      301 gccgaagtgg acccggtagc cgccggcgtt caggccggtg tcgagatagg cgccgtcggc
      361 gccggtgtag ccggccgaga ccaggtagcc gttgaggtca tacacttcga cccgcacccc
      421 ggcgatgggg ctggcgccgt cggtgacggt gccgccgatg gcgcccgggg tgaccaggac
      481 cgcgtcgatg ccgttggtcg gggcgggcgg gatgaccgcg accgggtcgg catcctggat
      541 ggcaggacgg tcgttgtacc actcgtagat gtagccgtcc ttcatgaaga gaacccggta
      601 attttcggcg gagaggcccg agaaggagta gtggcccagg gcgtcggtct gggtgaaggc
      661 gatctggaag ccgagcgaac tgtagaggta gacgttgacg gcggccagcg ggctggcggc
      721 aacgtcggtg acggtgccgg agagggtgga gccgtagatc agggagccgt tgacggtggc
      781 ggtcgccgag gcggtcaccg tcaccgggtc ggcggacgaa aaatcggttt tgccgttata
      841 ccaaccggcc aggtagccgc tgctgccggt ctggaagtag acccggtaaa cgccggtctg
      901 gaggttgtcc acctggtaga agccgttgag gtccgtctgg tccgtgttga ccaggccctg
      961 cccggcgtcg tagatgtgga ccgtgattcc ggcgatcggg ctgccggagg actggtcgcg
     1021 gacggtgcct tcgatggccc ccttctggct cagggcggcg tcgatgtttt cggtggtctc
     1081 cggggcggtg acggttatgg tgtcggccag gctgaggtcg gtccggttgt cgtaccattc
     1141 gcccaggtag ccgaagagga agaagccgac cttgtactgg ccgctggtca gcggcgaaaa
     1201 ggaatagttt ccctggccgt cggtgctgct ccactggatg gattggccgg cggcgtcgta
     1261 aagggtgacc gagacgccgg acaggccgtc gccggtggcc tggtcggtta cccggccggt
     1321 gatgcggccg ccccggagga gagagccgtt gatgttttcg acggtgccgg gggcgggggc
     1381 ggctacggtg tcggcttcgg cgaaactgga tttcccggag taccaggagt tgaagtagcc
     1441 gttcttgctg aactggacct tgaagttggc gtcggggagg ccgtcctcga cgatgtaaac
     1501 cgtatagttg ccctggctgt cggtctgggg cgggttccag gagttgaggg cgccgccgcc
     1561 ccagctgacc gggtggacgg ttaccgcgac gccggccagc ggatcgccgg tggcggcgtc
     1621 ggtgacccgt cccttgatgg cggcgccccg ggccaggacc acgtcccggg gcaggactac
     1681 ctgctggctg tcattctcga ccaggagggt gtcggcggtg gccaggtcgg tccggttctc
     1741 ccagaattcg ttgaagcagc cggccccctc gccatagaac cagaggcggt agacgcccgg
     1801 ttccaggttg cgcaggatga aatggccggc gtcgtcagtg gtggccgaat cccagctctg
     1861 gctgtccgag gcgtcgtagt agtaaacctg gacgccggcc accgggcggc tgccgtcggt
     1921 gacggtgccg gagatggagg cggacgggtt gagcaggacg tcgcccaggt cggtggcggc
     1981 cccgagagtg acggtgacgg tgtcggccga ggccgcgtcg gcacggtcca ggtaccactc
     2041 ggactggtag gcgccgttcc aatcgttgaa ggagaccagg tagtcgcccg acgggaggcc
     2101 ggccacctgg aagttgccgt ggtcgtcgct gaccgcccac cagggcgccc cccagaagaa
     2161 ctcagtgtcg gcctcgtaga cctggacctg gatgccggcg accggtatgt tgctgaaggc
     2221 atccttgacc cggccggtaa tgcggccgcc ctcggccagc tggaaatcgg cctgtttcag
     2281 gcgcccttct tcggtggcgg tgtccacctg gatggcgtcg gcagcctcga aggaatactt
     2341 gccgctgtac cactgggcgg catagacgct gttgaagagg ttggccttga ccttgtagtt
     2401 cccggcctcc aggtactcca gggcgtagta cccctggagg tcggtttcgg cccagcgctg
     2461 ggatgtttcg gaagccgccg ggtagaggtt cacctggacc ccggccaggc cgcggccgtc
     2521 cggaccggtc acccggccgt agatgtggcc gccgttcatc tcttccagga ccacgtcgat
     2581 gccctcagta tgggcgccgt cggcgacggc caccggggtc gattcggcct ggctggccgc
     2641 gttctggtac cactcgccgt agaagccggt gccctcggcg tcgaaattca cctggtaatt
     2701 gccggccgtc aggccggtca gccggtagta gccgtcatcg tccgtctcgg tgtaagagta
     2761 accccaggcc tggtcctgct ggtaggcggc aacctggatg ccgggtatgc cgatgccggt
     2821 tccggaggtg acccggccca ggatggccgc ggccggactg aggacggcgt tgatcccggg
     2881 cgtgtcgccg ggagcggtta ccgcgacgct ggtggcgttg tcgaaaccgg acttgttgtt
     2941 ataccagctg ctggaatagc cggccgagtt gccgctgaaa tagacccggt agttgccgct
     3001 ggccaggccg ctggcccggt aggcgccgcc ggtcccggtt tcggtctgga cggcgagaaa
     3061 gccggacgag ttgtagacct ggacctggag gccggccagg ccgtcggtgg cgtcggtgac
     3121 ggtgccggag atggagccgc agccggccag gacggcgttg acgccgctgc ggaggctgcc
     3181 ctcggtcacc gcgaccagct cggcgctgtc gctgccggcc gcatcgttgt accactcgtc
     3241 gtagtagccg agccgggcgg ccgagaaggc gacccggtag ttgccgttgg ccaggccgcc
     3301 ggcgatatag tttccgtcgg tgtcggtttc ggtttcggcg accgaattga agaccttgtc
     3361 ctcgaggtag acccgcacct ggacgccggc gatgccgttg gtgccgtcgc gggtcacccg
     3421 gccgccgatc cggccgcagg cgttcagcac gacgttctcc ggcagggcga aactgccgcc
     3481 ggcggggacg gtcaggcggg tggcgacgcc cgggaagtaa cggtccttgt aaatttcatt
     3541 gaagtacccg atcgcggcgg cgtcgaactc caggccgtag gtgccggccg ccaccgtcag
     3601 ggtgaagttg ccgccggcgt cggtcagggc gctggcgctc ggactcgaaa ggttgtagtc
     3661 cacccggtag gcgttgaccc tgacgccggc gatgccgttg gtgccgtcgc gggtcacctt
     3721 gccggtgatg ctttcggccc gggccgggcc ggccaggctc agcaggcctg gcagggtggc
     3781 cagcaggccg acagcgatga gtttggaaag aaaaggcctg taaccgggta tggctggcgg
     3841 tttgatgccg gtcataaaac cctcctgatg attaatttgt ttaaggtatg gatatgatac
     3901 gggcacaaga aggtttgact caggggattt gtcattgtgg gttatgcatt tttcggcatt
     3961 attatgccct ttaaaacata aaaaattcaa agataattta cctttaaaga gctaaattgt
     4021 caactcaacc tgatctcttc ctgatggtct tgatgatttt ttatcgaagt gggtatgccg
     4081 ataagctgcc tggtcctggt gtttagccgc tcataccaga acttacgtca accgtaattc
     4141 cgaaatcggc atgtccgttt tcgcatcgga tgctttcagg ggcgcttacc cgccatcgca
     4201 agccttcgga atcgtcactc gcgatgctgt tatccgttat cagttccgcc ggttagtgcg
     4261 gccggctctt tcaactactt atattttagc ccaccacctc ttgttgctgg tttctgtcgc
     4321 tccagaagtc gggcaatgtt gacgctcacc cccgcgcttg gtttttcact tgccagggac
     4381 agtcccgctt aattgatgcc tgagactatg cccgtcggca gccgctttgc tttttaactt
     4441 tttaaaggcg tcgataattt gcgtgagtgt ccaagaggta tttgctgttc tttcgcttat
     4501 tgttttctgc cagagtcgga gtcttgcttt taaatgatgc gtttttgagg aaagctcagg
     4561 cccaagccgt ctatcggccg tggtctgatt ttcaaaatgg gtgttgccgt atggtgtttt
     4621 tttcaaaaat gggaacggtt tttatttgac aatggaggtt gtggttcggt aaaatagata
     4681 aaaatttcat gtttttatag ttgttgggga ttttcgggga aattaaacaa taccatagag
     4741 gtgcgattgt cgttaatgag cggaggcgga ttgtgaaaat tgaaacctcc tttcgggtgg
     4801 acgctctctc cttgttgctg tctcttggcc tgttaacggc cgcccggggg gccggcgtct
     4861 cggtcggcga cctcgacgtg accggcctga atgtcgggga taccatcgaa ttgccggtcc
     4921 tggtcagcgg agtggtcccg gaggagttcg ccggcttcca gttcgagttt gatttcgacc
     4981 cggcggtact ggagttccag ggggtcggtc acggctccct gatcgccatg gaggatgccg
     5041 cttacgcggt ggctccgtac agcccgaacc tctgggcgct cgaggccaac gccaacgcct
     5101 cgaagctgct ggtggccggg atgatcctga tgaacgtcga cagcggccac cttgacctgc
     5161 tccagctggc ccggcccgct tccggcgagg gcgtgctgct ccggctccgg tttcgactgc
     5221 tccagaaggc ggagtcgctg gtgagcgtca ccggcaccgc cggccgcaac ctgctggcct
     5281 tcgataccct ccagccggcc gccagtccga gcacctcggc cccttcgccg ctgctggccg
     5341 gcggcaccga caccaacggt atgggcaatg gctgggaact tctttacctg ggcggcatcg
     5401 gcaacgatcc ccggggcgat ttcgaccggg acggcttctg gaacatccag gagttcctgg
     5461 gcggcgccct gcccaacaac aacacccagg ccggcctggg cgtcaacctc ctgaaatcgg
     5521 ccaccaccgg tttgaacctc ttctcgattc ccctggccga tcccagcatc ggcgacaccg
     5581 ggctggcccg ggccgccgac ctggtttcgg cgctgggcgg cgcctcgaaa gtgggcgtca
     5641 tctacaagtg gaatccggcc acccagggct ggaccagcca catcgccagc ctgccgtcca
     5701 tcaacaactt cgacctgcag gccgggatgg gcctgatggt ttcggtcaac cagaacattt
     5761 cggcccggct gctggggacg ccgacccgct tttcggcctc gctggtcaag aacgccgaca
     5821 agacgagctt gaacatcatg gtactgccgg ccggcctgac caacccgccg gccaaggcgg
     5881 ccgacctggt cgcggcgctg ggcggcgcct cgaaagtggg cgtcatctac aagtggaatc
     5941 cggccaccca gggctggacc agccacatcg ccagcctgcc gtccatcaat aacttcgacc
     6001 tggagccggg ccagccgctg atgatctcgg tcaaccagaa catcgagtgg ccggccgccg
     6061 gcgactggcc ggaactttga agctctgagc cggacctgaa aacggtcatt tgctttcaac
     6121 tttggataga atacccgtca aaccacagga ggattggatg tcgatcttga agaagcttgg
     6181 cctttcgata atgcttgtca gccttttatt gctgggcgcc ggcgcggcgg cctcctttgc
     6241 cgccaacccg cactcgatcg tcgtgacggt caccaactcg aacagttcga ccccggccga
     6301 cggcgctctg acgatgaacg cctacatcca ggtccggccg gccgagatca tcaccctggc
     6361 cagtcccagc cagcaatata ccggcggcaa tctcctggtc aacctggccg atttttcggc
     6421 ctgggcggcc ggcgaggtgc tggtgattca gctgaccaac accgtcaacc aggaggtcgg
     6481 cagccttaac gtgaccatca ccaacgatga tccccagttg gccaacctgc agctggtggc
     6541 cggcaccggg accctgatcg tgcgcagcaa cctccaggcc ggttcctaca ccatcaccgg
     6601 tccggccggt ttcaacggca acctctccga cggcaccctg agcggcgggt attaccagaa
     6661 gaccctgagc gcctcgactt cgatcggcag ttacacggtc gtctgggggg cggtggccgg
     6721 ctacttcacc ccggccaacg agaccctcaa cctggccaag gacggcagca tcaccttcac
     6781 cgggacttac aaccagatac ccagctacaa cctgaccatg gccgtcagcc cggccggcgg
     6841 cgggaccacg gtgccgacga ccggcacctc cagccgcctc cagggaacgg tggtcaacat
     6901 ttcggccact cccaacgccg gctaccgctg gaacaactgg acggtcaatg ccggtgtcaa
     6961 cgttgacacg ccggccagcc agaacaccag cgtgacggtc gacgtcgcca agaccgtcac
     7021 cgccaacttc atcaagacct acacgctgac ggtcgggtac accggcagcg gccaggtgag
     7081 cggcaccacc accggcgtct acgacgtaaa cacgccggtc agcctgaccg ccaccccggc
     7141 cgagcactgg cgcttcgtaa actggacggt caacagcgcg gtggtcagca ccagcccgac
     7201 ctacaacacc acgctcgacg ccgacaaggg catcgtggcc ctctttgagc cgatcaactt
     7261 cgtcctgacg gtcgccgcca gcccggccgc cggcggtgtc gttaaccgcg acccggccgg
     7321 gacccaggtg gccgccggcc agtaccagta ccaggagggg acccaggtca gcctgacccc
     7381 ggtggcggcc gtcggttatc agttcaagga gtggcagggc ggcctgaccg ggagcaatgt
     7441 ccccggcgtc ttgacgctca atgccaatac cgcggtgacc gcggtcttcg agcagattcc
     7501 ctacctctcg atcaacaaga ccgagattgt cttcaccgac accaagctga ctgacaccct
     7561 gatcatcacc aacaacggcc agggcctgct ctcctgggcg atcgatgtca accagaatgc
     7621 cgggtcgggc ctgaagacgc cgatggtggg ctggctaaac gtctccccgc ttaccagcgg
     7681 cgatgtcccg ctggcggccg gccagtcggc ggtggtgacc ctgacggtca tccagaacaa
     7741 cagttacgcc gagctgacgg tgcgcaacac caccgcgggc atcaaccagc cggaccagct
     7801 ggtgatcgcc cggatcaacc aggcgccgat cagtccggat ccggacgggg ttgatttcca
     7861 ttacctggat gcggccgcgg cgccggtggg cgaggctatt gacctggaca cggtcggagt
     7921 ggacacgccg atcatcatca ccggcccctc cttcgacgac gccgaccaga acgattacca
     7981 gatcgcccgc gagtacgaaa tctggaccac ggccacgatt ccgaccgggg tcttcgagcc
     8041 gcagcccttt gtcttagacc agaaggtctt cggagagagt atccagggca cccgcgagtg
     8101 gacctccttc ggccggaaca tcctgaagcc ggagaagacc tacggcctgc ggatccgcta
     8161 ccaggacaat tacagcgcct ggtcggaatg gaccgattgg attctcttca agaccgttgc
     8221 ggccggggag accctggatg accagggcca gggcgtgccg tccgaccagt tcgtcgacct
     8281 gaacacggtc gatctcgaga agtatcccta cctggccggc cacgacaacg atgaaacgga
     8341 tgccaaggtg gtgctggcca ccgaggcggc caccggcgat ccggtcccgg tactggtgga
     8401 gaccaacacc ggcgagctgg tctacctgcg ccaggacgac ctgggcgctc cgaccgcctc
     8461 gaccacggcc ccctacgggg tcttcaccac ccgggtggag aacatcgccg ccggggccga
     8521 ggtgacgctt accttcacct tcccggaggc gctgccggcc gacatgctct ggtacaagta
     8581 cgacgagacg accgccggct ggacgcccta tgacgaggcc aactgggact tcgggccggc
     8641 cgccaaccag gtgaccgtcc ggatcaagga cggcgactac ggcgacgccg accgggtcga
     8701 gaaccaggtg gcggtggatc ccgccgggcc ggtgaccctt ccctcgggcg gggctctcgg
     8761 cagctactcg ggcggcgacg gcggctgctt catcgccacg gccgccttcg gttcctacca
     8821 ggagcgccac gtctggatgc tgcgcctctt ccgggaccgc ttcctgctca ccaaccggcc
     8881 ggggcgggcc ttcgtccgtt tctactaccg ccactcgccg gccctggccg gctggatcgc
     8941 gggccgggaa tgggcccgtt cggcggcccg gtccctgctg atgccgcttt acggggcggc
     9001 ctacctgctg gtcaacggcc tggccgggat gttggccctg gccgccggcc tggccggcct
     9061 ggctgttctg gtcgtccggc ggcgtcgggc ggcggtcctg gcggccgcgg cggcctgcgc
     9121 gctgctgctt ggcaccgccg gcccggcggc tgcgctcgat gccaaccacc tggtgccggc
     9181 cgtcggcgag tcctccctgg tgcggaccta caccgccgac ctgctgccgg ccgggaactg
     9241 gcagctgggc ggcctcttca actatgtgag tactccgctg gagggaaagg tcaacggcgt
     9301 cgagaaggag ctctccgaag cccagtatat cctgaccgtc tcgggcgcct acgccctgaa
     9361 cgagaagtgg cagctgggcg cctccttccc ctggctggtc aagcaggagg gcgagctgac
     9421 cggcgtcgac gaggcggtgg aatcggccgc cggagacctg cgcctggagg ccaagtaccg
     9481 tttctgcggg ggcaaggacc agctgggtta cgccctggtg ccgttcctga ccctgccgac
     9541 cggtgatgac gacccggccc ggggcttctt cggccgcccc tcggccagtg ccggcgtcat
     9601 cttcgtggcc gatcggaact ggtgcaacca tacccacgtc gccctcaacc tgggccttca
     9661 ggtccaggag gaggaactcg accggatgac gatcgagaat acgcttctct ttggcctcgg
     9721 gctgagccgc ctgctttcga acgaaaggac caccctggcc ctggagatca acggccgggc
     9781 cgatgacagc tggttctccg aggcggagtc gacgccgatc gagatcctgg gttcgctcac
     9841 ccagcaggtg aacgagtcgc tgcgtttgag cttcggattg gggagcagcc tgaacgacgg
     9901 gtatggggcg cctgattacc gggtgctggt gggactgaaa gcggctttct gaaattaagg
     9961 ttttactgaa aaaagagaag ggcggcctcg ccggaaggtg ggccgccctt ttttattcgc
    10021 cggtacaatt tctcaataat tctggaacgg gttgtcgtct gacttggtct cctcggccgg
    10081 cggagtctcc tcggcttgct gtggttcagg cggggccgct tccacggcgg tcggcggagc
    10141 ggtttcggcc tccttctcga ggccgaactc attctccggg agtttcacgg tgatccggcc
    10201 cgcggcggcc cgccgggcca ggccccgggc ggccgcccag cgcaggtcga ggttgtcatc
    10261 cttcaggagg ccggccagga tgtccttgac cgagggatcg tcgatccggc ccagcgcgtc
    10321 ggccgcggcc agccgcaggg tgggctcgtt ctccttgagc atgtccagca gggccggcat
    10381 ggccgcccgg ctgcccatca tgccgagcga ttcggccgcc gcctggcgga tgtgcggatt
    10441 gtaattcttc agcagatggg tgagcggttc caccgcccgc tcatcgccga tccagcccag
    10501 caggtaagcg gtgttcctgg ccatcaggac atccttttcg gcccccttga gaatttcgca
    10561 gaggggttcg acggccgcgc cgcccttgcc caggagcttg ccggccgcgg tttcgacgtc
    10621 ggtccagctc ttgccgccgg tgaaggcctt gagcaggtgc tggacttcct tgtcgactac
    10681 cggtttgggc ttcttttcgg ggcgggccgg cttctcccgg cgcggcggcc gggccggggg
    10741 cgccgccttc ttttcggcgg ccggcaccgg aagttcggcc tgcttcggct cggccggctt
    10801 gccggccccc ttcttcaatc caaggttaaa tttcatgatc gcctccggtt agcgttgaat
    10861 cagcctgaca gggtccggaa gaggttccag gcgatgatca ggtgaccgtg ttcattggga
    10921 tggaccggtt cggtgcccag ggtctcgggg tcccgctcct tcagctgggc ctcgaagatg
    10981 tcctggaggc ggaccagcgg ggccttgaac tcccggctga gccgggcgac cgcctgccgg
    11041 taggggcgca attccttgag aacccggccg cgccagccgt cggtccggtc ggtcgacaga
    11101 taaaagggtt cgagcaggat catccggcag ccgacctccc gggtggcgcg gtccaggagt
    11161 tcccggtaat tctccaggaa acggtccggc cccagctggc cggcgtcggc ggcgccgcgc
    11221 agcacgtggt gcaggtcgtt gatgccgatc agcacgctga gccagtcggg acggtggtag
    11281 gtgacgtcgt cctcccagcg ctgcttcagg tccaggatgg tgttgccgcc gatgcccttg
    11341 ttcaggacct ggacgttgag atcgggaaag gcggcctgca ggagagtgtt gaagtggcgg
    11401 acgtaaccat tgcccagcgg cggaaattcc ttgcggcggc cgcagtcggt aatgctgtcg
    11461 ccgatgcaga ggaaccggct gttacggccg accgggaatt ttctggcgga tgcagccata
    11521 tttttcgcct caaatgtatg tgtgcgattt tggacccggt tggtcagttc cgggtgttca
    11581 cttttggtat tttagcaaat cgccttcaaa gcgtcaaccg gacgcggcga cagttgatgc
    11641 ctgcttgaca aaccagccgg gggggggggt attatgagat cgaaattgat atgcttgcat
    11701 ttctgatcac
//
LOCUS       DAOQYN010000030        11641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_5990_length_11641_cov_4.629294, whole genome shotgun sequence.
ACCESSION   DAOQYN010000030 DAOQYN010000000
VERSION     DAOQYN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5990_length_11641_cov_4.629294"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..238)
                     /locus_tag="PKN80_02240"
     tRNA            complement(168..238)
                     /locus_tag="PKN80_02240"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gln,seq:ttg)
     gene            complement(321..1877)
                     /gene="purH"
                     /locus_tag="PKN80_02245"
     CDS             complement(321..1877)
                     /gene="purH"
                     /locus_tag="PKN80_02245"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNQ34864.1"
                     /translation="MITIRRALLSVSDKAGIVDLARALAEHGTELVSTGGTAKALREV
                     GLAVTEVSELTGFPEMLDGRVKTLHPKIFASLLHRRDLPDHLKQVAEAGIQPIDLVAV
                     NLYPFEKVVGLGCKPETAIENIDIGGPSLLRAAAKNGRDVVVLSDPGQYPQAIADLKA
                     NRGRISRGLAQQLAAAVFARTAAYDAIIHRYLSRESEPAAGFPPVLLFRLDKVGELRY
                     GENPHQPAALYREEDFRDAPGRLVDAEKIQGKELSFNNYLDLEAAYRLVREFDQEAAV
                     IVKHNNPCGVALGTDIAVAFRRARACDPVSAFGGIVALNRPVTEEAAREITTGFVECV
                     IAPEYAPAALKQFQKKPAIRVLRLNWGRPAAGGLDLRPVGGGFLAQEPDASLYSELKV
                     AGRREPTAAQLEDLKFAYTVAKHVKSNAIVLVRDGQTIGIGAGQMSRVDSARIAVSKA
                     REFGFEIKEAAVASDAFFPFPDALEVTAEAGATSVIHPGGSKKDAEVLAAAEKHGMVM
                     IMAGQRHFRH"
     gene            complement(1946..2866)
                     /locus_tag="PKN80_02250"
     CDS             complement(1946..2866)
                     /locus_tag="PKN80_02250"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNQ34865.1"
                     /translation="MAWTRKHLLSIEELSAAEINLVLETAASFKEVSTREIKKVPALR
                     GKTVATLFFEPSTRTRTSFELAAKRLSADVVGFSPSTSSLVKGETIIDTGENLEAMKV
                     DCFVVRHASSGVPALLARKLKASIINAGDGNHEHPTQALLDLLTIQEHKKRFRGLKVA
                     ILGDLRHSRVARSNIWALTKLGAEVILCGPPPLIPPGLETTGARVTVRRETALKDADV
                     IYILRIQLERQQENYFPSLREYSRYYQVNRETLSLARPDAIVMHPGPMNRGVEISTEA
                     AEDRRSVILNQVENGIAARMAVLYLLLVGK"
     gene            complement(2881..3426)
                     /gene="pyrR"
                     /locus_tag="PKN80_02255"
     CDS             complement(2881..3426)
                     /gene="pyrR"
                     /locus_tag="PKN80_02255"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNQ34866.1"
                     /translation="MEKKIEVIGEAEIGKLINQLSESVSRTLNLENLAILGIRRRGVE
                     LALRLQKRLEKPGRKIPLGILDITLYRDDLSSVDVQPLVRGTSIPFDLTGRDILLVDD
                     VLYTGRTIRAALTQLVDFGRPRSVKLLTLIDRDHRELPIQPDFTGRFVEAAAGEFIEV
                     RLREVDGEDKVLLITRPDRAS"
     gene            3604..4266
                     /locus_tag="PKN80_02260"
     CDS             3604..4266
                     /locus_tag="PKN80_02260"
                     /inference="COORDINATES: protein motif:HMM:NF019378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HNQ34867.1"
                     /translation="MLLLPPLILLVLFFLLLLLPVLLILLPIRIVEAALGRLGIPGPL
                     ALLLFLASLLGSFVNIPVLGAAEGTGRLVLMPGLQALGPFFNAPSCPQQVLAVNIGGA
                     LVPLLICLFLLPRAPLARTLMATAVMVLVCYLVARPVPEVGITIPTFLPPLAAVLCAF
                     IFSPGRRAPVAYIAGVLGVLIGADLLHLADFPPGPGFLSIGGAGVFDGIFLVGIMAAL
                     FA"
     gene            4303..4809
                     /gene="tpx"
                     /locus_tag="PKN80_02265"
     CDS             4303..4809
                     /gene="tpx"
                     /locus_tag="PKN80_02265"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNQ34868.1"
                     /translation="MRSITIHGRPVPLAGEGVKAGQEAPDFTVVDPNHRVISLKEFKG
                     RIKIIASVLSVDSPVCNLEIRHLNQAAAGFEADTMFIFVSMDLPFAQRRFCQANAINR
                     MKCFSDYRKADFGLKYGVLIEESRLLARAFFVLDRNDRIHYCQYVPELTQPPDYDALL
                     ESLRSMAG"
     gene            4952..5476
                     /locus_tag="PKN80_02270"
     CDS             4952..5476
                     /locus_tag="PKN80_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNQ34869.1"
                     /translation="MEAGLRVLVVYYSWTGNTRRVAEAIARQAGAEIREIVEKQPRRG
                     VRGWLSGARDAARKRPSEIEPPPEGNYDLVIVGTPVWAFTMVPAVRAFLVQSRPRLRD
                     LAFFCTQGSSGAEAAFREMEALGGRPPLGTLTVNVPELKDAEWEARVRKFVEEIECNL
                     RLKPGREIERPEGE"
     gene            5477..6109
                     /locus_tag="PKN80_02275"
     CDS             5477..6109
                     /locus_tag="PKN80_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34870.1"
                     /translation="MGNKEKRRVILVAQCLLNPYCRVHILGQNFPFSRELSEYLMRKK
                     IGIIQYACPETTAMGLMRNPQGRQQYNNIFFRDHCRKLLEVPLLMVREFLENRYRLVA
                     FIGLENSPTCGIHWGKHKVNRYRTESPEKVTELRPGEPVLMGIMAEMLSAEFNRAGIS
                     VPFLEFPANSTAESPERRKFWTELQRIVEPYLFEEDGKPASAGKEAAAGD"
     gene            6142..6330
                     /locus_tag="PKN80_02280"
     CDS             6142..6330
                     /locus_tag="PKN80_02280"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNQ34871.1"
                     /translation="MFKMIEIVAVSPQGFSEAAAAGVEQLASEGFKIHFFEVVELRGA
                     VREGRVKEFQVKIKAAVE"
     gene            6397..6813
                     /locus_tag="PKN80_02285"
     CDS             6397..6813
                     /locus_tag="PKN80_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015174858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNQ34872.1"
                     /translation="MRGRIIAKIRERAAALERETWVLYRAYRDPRVSRPARLIIALVV
                     GYAFSPIDLIPDFIPVLGYLDDLLLVPAGILLALRMIPPEVLAECRARVREDFEAGRR
                     PGRWAAVVIVIIWLLLIVLLFRWLSRLAAGRAGGSS"
     gene            6810..7460
                     /locus_tag="PKN80_02290"
     CDS             6810..7460
                     /locus_tag="PKN80_02290"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNQ34873.1"
                     /translation="MKYLSAAEMRALERRAIEELGIPALRLMERAGQALAGEAFRLAG
                     VGNYLVICGYGNNGGDGLAAGRLLAERGGRVRLFLVGPPRPFTAETAANFEALRKTGL
                     AAGRLEKTADLEVLEPPEPPAAVIDAIFGIGLRGPLEGFYADLVERINSLGVPVAAAD
                     LPSGLDADRGEPLGCAVRASVTVTFGHPKLGFRNPAAAAYLGRVVVADIGLPPDNL"
     gene            7554..8585
                     /locus_tag="PKN80_02295"
     CDS             7554..8585
                     /locus_tag="PKN80_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34874.1"
                     /translation="MLKKQFCRAALLAFLLLWSFDGEAHLCDNVFRQQDKLIVKPENY
                     NLVVKDRLTFKIFLQNNMDRGIAEISLQADSPAFDFQITPARMSIPKDQRAFFEVTMS
                     PHPGVASGNYGVNFRLVGGGREFKRFSLNTGGEAAGPAAGTEPGRTAPAESKASRFQV
                     RAMSTPPQVDGQPGDPAWKGAAVASKFSSRAGGNAVNQTYGLLGYDSSRFYLAVFCRD
                     ERPEQIGLRDQVRIWLAGPAGAPVYGVTISAAGSQTTSRIEASGKQSAWSPAGLKQSV
                     SRQKDGWLVECSIPFSDLNLSGPQLVRPWFLRLERFKASGVSEESYWSADASGFNSER
                     GYGQFIMAP"
     gene            8610..9152
                     /locus_tag="PKN80_02300"
     CDS             8610..9152
                     /locus_tag="PKN80_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34875.1"
                     /translation="MKRILINAGILFLLCLSAAGASAAERLEISFTYPSFKGDFYASG
                     LVAFPPGAVGDENQLKVVNAAGLEVATNVKVRETWFDGSLLSADITFAANSSRPDRYH
                     LVYGGDTLRAKRLGETAVLPVSAFSVGGAARTVETMDMPVGQINVRVDRSPDIRYYWH
                     LVPLTALLFLTVIRSRRTSR"
     gene            9149..10201
                     /locus_tag="PKN80_02305"
     CDS             9149..10201
                     /locus_tag="PKN80_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ34876.1"
                     /translation="MKPRRRITLSRRIIQLACLILLVYGGYLFKGRLAGQEANVGEPP
                     GTGGSLFPDLKAPKGAMSTTQFAPGSILWPSGATPVLENYPPTLVCRFNPKGGLFKAC
                     IVHFFSENFTWQTHLKYLLPHLTLFVLLCFLLGRFWCGWVCPIGSLGDLLSWLRRRMN
                     VPVRRFPAGFRNALVFSSYGLLGATLGISTFIGLPNYSRFQCYWFLPYCQICPARLVC
                     PVFGLIKPGWKDFTNGVSATFTLLAWLVLGLFVAAFYWGRRVWCHLCPVGLVNSWFNR
                     GAGLELHKKAVKCNKCGYCADACPMGLTAMYEADTDRKYNQNGCIMCLRCLEVCPKDG
                     CLSCRFFGRRVLESRY"
     gene            10212..11459
                     /locus_tag="PKN80_02310"
     CDS             10212..11459
                     /locus_tag="PKN80_02310"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNQ34877.1"
                     /translation="MKKHQDQGGLKPEYASILDGFYRGLAPSGLSPDYRAETEVYRRR
                     SRLASAGRLYESLMSASGVAAGPRVGYFCNLVPEELIRACGAVPVRLCELDHACARLG
                     EEVLPGDACPMIKSICGSAAAGRYRDLDLLVIPAACDGKAKLSELLAPVRPVYLLDLP
                     RSSEYLRNADAWTGRYQDFFDFLKERFRSRAGRSELTAECRRANLRTQLFRRLYHFRA
                     GHPGLINHFDYFTLVSLSFRLEPEAWLDLAGEILAEAVGRAEGLKFDGRRILLAGSPI
                     IFPAYKVLDILYEFNCEAVADSLCSGYGHLYDPVEIDEETETGIIRALALKYLAASLC
                     PCFMGLNKLLDLLVDTTAEYRLDGVIYHDLRLCQVFDMQTVAIRQVLKEKRIPFLSIK
                     TDLGREDTGQLKTRIEAFLEMLG"
BASE COUNT         1828 a         3692 c         3855 g         2266 t
ORIGIN      
        1 ggaattcgct ggtttgtctg aataagattc agaaacaaca atcgccatga agcagcgcga
       61 agcacgcttc atggccgtga agtgtgttcc gagcgtgtcg aggaactcta cttcacggct
      121 cccatcgaat cctcaagcac cgggcttcag gagggtatgg caggtagttg cccggtgagg
      181 attcgaacct caacgcccag ctccaaaggc tggtgtgcta ccattacaca accgggcagg
      241 atcataaggc cgccggcctg atcggccggg agatatttag tccgactgcc ttacatcggc
      301 gtttaagggc cgggccgccg ctaatggcgg aagtggcgct ggccggccat gatcatgacc
      361 atgccgtgct tttcggccgc cgccagcacc tcggcgtcct tcttggaacc gccgggatga
      421 atgacgctgg tggcgccggc ttcggccgtt acctccaggg cgtccgggaa ggggaagaag
      481 gcgtcggagg ccaccgccgc ctccttgatt tcgaagccga attcgcgcgc cttggagacg
      541 gcgatccggg ccgagtccac ccgggacatc tggccggcgc cgatgccgat ggtctggccg
      601 tcgcgcacca gcacgatggc gttgctcttg acgtgcttgg ccaccgtgta ggcgaacttg
      661 agatcctcca gctgggccgc ggtcggctcc cggcggccgg cgaccttcag ttcgctgtag
      721 agggaggcgt ccggttcctg ggcgaggaag ccgccgccca ccggccggag gtcgaggccg
      781 ccggcggccg gccgtcccca gttcaggcgc agaacgcgga tagccggctt cttctggaat
      841 tgcttcaggg ctgccggcgc gtattcgggc gcgatgacgc actccacgaa gccggtggtg
      901 atctcccggg ccgcttcctc ggtcaccggc cggttcaggg ccacgatgcc gccgaaggcg
      961 ctcaccgggt cgcaggcccg ggcccgccgg aaggcgacgg cgatgtcggt gccgagggcg
     1021 acgccgcagg ggttgttgtg cttgacgatc accgccgctt cctggtcaaa ctcgcggacc
     1081 aggcggtagg cggcctccag gtcgagatag ttgttgaagg agagttcctt gccctggatc
     1141 ttttcggcgt ccaccaggcg tcccggggcg tcccggaaat cctcttcgcg gtaaagggcg
     1201 gccggctggt gcgggttctc cccgtaacgc agttcgccca ccttgtcgag gcggaaaaga
     1261 aggaccggcg gaaacccggc cgcgggttcc gattcccggc tcaggtagcg gtggatgatt
     1321 gcgtcatagg cggcggtccg ggcgaagacg gccgcggcca gctgctgggc gagcccgcgg
     1381 ctgatccggc cgcggttggc cttcagatcg gcgatcgcct gggggtactg accggggtcg
     1441 gagaggacca ccacgtcgcg gccgttcttg gccgcagccc gcaggaggct ggggccgccg
     1501 atgtcgatgt tctcgatggc cgtttccggc ttgcagccga ggccgaccac cttctcgaac
     1561 gggtagaggt tgaccgccac caggtcgatc ggctggatgc cggcttcggc cacttgcttg
     1621 aggtggtccg gcaggtcgcg gcggtgcagc aggctggcga agatcttggg gtggagcgtc
     1681 ttcacccggc cgtccagcat ctccgggaat ccggtcagct cggagacctc ggttacggcc
     1741 aggccgacct cgcgcagggc cttggcggtg ccgccggtgg agaccagttc ggtcccgtgc
     1801 tcggccaggg ccctggccag atcgacgatg ccggccttgt cggataccga aagcagggcg
     1861 cgtctgatgg ttatcatacg atacctccgg ttttaatggt tggcggcggg gatccgccgg
     1921 atggaatcaa atggttccga aacgcttatt tgccgaccag cagcaggtaa aggacggcca
     1981 tgcgggcggc gatgccgttc tcgacctggt tcaggatgac cgagcggcgg tcttcggccg
     2041 cctcggtgct tatttcgacg ccgcggttca tcggccccgg gtgcatgacg atcgcgtccg
     2101 ggcgggccag ggagagcgtt tcccggttga cctggtagta gcggctgtac tcccgcagcg
     2161 aggggaaata gttttcctgc tgccgttcga gctggatgcg caggatgtag ataacgtcgg
     2221 catccttcag ggccgtttcg cgtcggaccg tcacccgggc gccggtggtc tcgaggcccg
     2281 gcgggatgag cggcggcggc ccgcagagaa tcacctcggc gcccagcttg gtgagggccc
     2341 agatgttgga gcgggccacc cggctgtggc ggaggtcgcc caggatggcc accttgaggc
     2401 cccggaaacg cttcttgtgt tcctggatgg tcagcaggtc cagcagggcc tgggtcggat
     2461 gttcgtggtt gccatcgccg gcgttgatga tcgaggcctt gagcttcctg gccagcagag
     2521 ccggcactcc ggacgaggcg tggcgaacga cgaagcagtc aaccttcatc gcctccaggt
     2581 tttcgccggt gtcaatgatg gtctcgccct tgaccaggct cgaggtggag ggggagaagc
     2641 cgaccacgtc ggccgaaagg cgcttggcgg ccagttcgaa ggaggtccgg gttcgggtgg
     2701 acggttcgaa gaagagggtg gcgaccgttt ttccgcgcag ggccggcacc ttcttgattt
     2761 cccgggtgga gacttccttg aaggaggcgg ccgtctccag gacgaggttg atctcggccg
     2821 ccgagagttc ctcgatgctc agcaggtgtt tccttgtcca ggccatcttt tccgtgtttt
     2881 ttacgacgcc cggtccggcc gggtgatcag gagcaccttg tcctcgccgt ccacttcccg
     2941 caggcgcacc tcgatgaatt cgccggcggc ggcctccacg aaccggccgg tgaaatcggg
     3001 ctggatgggc agttcgcgat gatcgcggtc aatcagcgtc agtagtttca ccgagcgcgg
     3061 ccggccgaag tccaccagct gggtgagcgc cgcccggatc gtccggccgg tatagaggac
     3121 gtcgtccacc agcaggatgt cgcggccggt gaggtcgaac gggatcgaag tcccgcgcac
     3181 cagcggctgg acgtcaaccg agctgaggtc gtcgcggtag agggtgatgt ccaggatgcc
     3241 gagcgggatt ttgcggccgg gtttttcaag ccgcttctga aggcggaggg ccagttccac
     3301 gccgcggcgc cggatgccca ggatggccag gttttccagg ttcagggttc gggaaaccga
     3361 ttcggataac tggttgatga gtttgccgat ttcggcctcg ccgatcacct ctattttctt
     3421 ttccaccgat aaaatgataa catatcttta aggcggctga caatttacgg ccgaggcgat
     3481 tccagccttg attccaggcg cttccggggt tgccgggcga tcatttcaac cggcgggagc
     3541 ggaggacggg acgcccatgc gcggtccatt ggtttttccg gccggcgccg cccggggtat
     3601 catatgctgc tactgccgcc gctgattctg ctggtgctgt ttttcctcct tttgcttctg
     3661 cccgttctgc tcatacttct cccgattcgg atcgtcgagg cggcgcttgg ccgcctgggc
     3721 attccgggac cgctggctct gcttctcttt ctggcctccc tgctggggag tttcgtcaac
     3781 atccccgtcc tcggcgcggc cgaggggacc ggccgcctgg tgttgatgcc gggtttgcag
     3841 gccctcggtc ccttcttcaa tgccccaagc tgcccgcagc aggtcctggc ggtcaacatc
     3901 ggcggcgccc tggttcccct gctcatctgt cttttcctgc tgccgcgggc gccgctggcg
     3961 cgcaccctga tggccaccgc ggtcatggtt ctggtctgct acctggtcgc ccggccggta
     4021 ccggaggtcg gcatcaccat tccaaccttc ctgcccccgc tggcggcggt gctgtgtgcc
     4081 ttcatctttt cgcccggccg tcgggcgccg gtggcctaca tagcgggtgt gctgggagtg
     4141 ctgatcggcg ccgacctgct ccacctggcc gattttccgc ccgggccggg tttcctgagc
     4201 atcggcggcg ccggggtttt cgacgggatc ttcctggtgg gcatcatggc ggctcttttc
     4261 gcctgaattt gaacggtttt gaaaaatcaa ggggaggcgg cgatgaggag catcaccatt
     4321 cacggccggc cggttccttt ggctggagaa ggagtcaagg cgggccagga ggcgcctgat
     4381 ttcacggtgg tcgatcccaa ccaccgggtg atttccctca aggagttcaa gggacgcatc
     4441 aagatcatcg cctcggtcct ttccgtggac tcgccggtct gcaacctcga gatccgccat
     4501 ttgaaccagg cggcggccgg tttcgaggcc gacaccatgt tcatcttcgt ttcgatggac
     4561 cttccctttg cccagcggcg cttctgccag gccaacgcga tcaaccggat gaagtgcttc
     4621 tccgactacc gcaaggccga tttcggcctc aagtacgggg tcctgatcga ggagtcgcgc
     4681 ctcctggccc gggccttttt cgtgctggac cggaatgacc ggatccatta ttgccagtat
     4741 gtcccggaac ttacccagcc gccggattac gatgccctgc tggaatcact gaggtcgatg
     4801 gccggctgat cggccgggct gagaatcgta cgcagggttc gcctggccgg gccggccgcg
     4861 ccggtttttc cggccgtttt tattgttcag cccgccgcca gcaaggtcgg cgccggcgat
     4921 tccgcaggtg cgtgttgaca gggaggagtt tatggaggcc ggattgagag tgctggtggt
     4981 ttattactcg tggaccggca acacccggcg ggtcgccgag gcgattgccc ggcaggccgg
     5041 cgccgagatc cgggagatcg tcgagaagca gccccggcgg ggtgtccgag gctggctgtc
     5101 gggcgcccgg gacgcggcgc ggaagcggcc gtccgaaatc gagccgcctc cggaagggaa
     5161 ctacgacctg gtgatcgtgg gcaccccggt ctgggccttc accatggttc cggcggtccg
     5221 ggccttcctg gtccagagcc gcccccggct gcgcgacctg gccttcttct gcacccaggg
     5281 aagttccggg gccgaggcgg ccttccgcga gatggaggcg ctcggcggcc ggccgccgct
     5341 ggggacgctg acggtcaacg tgccggaatt gaaggacgcc gagtgggaag cgcgggtgag
     5401 gaagttcgtc gaggagatcg agtgcaacct gcgcctgaag ccgggccggg aaatcgaacg
     5461 gccggaggga gaataaatgg gtaacaagga gaaacgccgc gtgatcctgg tcgcccagtg
     5521 ccttttgaat ccctattgcc gggtgcacat cctgggccag aatttcccct tctcccggga
     5581 gctttccgag tacctgatgc gcaagaagat cggcatcatc cagtacgcct gtccggagac
     5641 gaccgccatg ggcctgatgc gcaatccgca gggccgccag cagtataaca acattttctt
     5701 ccgggatcat tgccggaaac tgctggaggt gccgctcctg atggtccggg agttcctgga
     5761 gaaccggtac cggctggtgg ccttcatcgg cctggagaac agtccgacct gcggtatcca
     5821 ctgggggaag cacaaggtca accgctaccg gaccgaatca ccggagaagg tgactgagct
     5881 ccggccgggc gaaccggttc tgatgggaat catggctgaa atgctcagcg ccgagttcaa
     5941 ccgggccggc atcagcgtgc ccttcctcga attcccggcc aactccacgg cggaatcgcc
     6001 ggagcgccgg aagttctgga ccgaacttca gcggatcgtc gaaccttatc tttttgaaga
     6061 agacggaaaa cctgcttcgg ccgggaagga agcggcggcc ggagactgag gtgtctccag
     6121 atcacggaga ggagcgcaac aatgttcaag atgatcgaga tcgtggccgt ttcaccccag
     6181 ggtttctccg aagcggcggc cgccggcgtg gaacaactgg cctcggaggg tttcaaaatt
     6241 catttcttcg aggtcgtcga attgcgcggg gcggttcggg agggccgggt gaaggaattc
     6301 caggtcaaga tcaaggcggc ggtggagtaa ggacgccggc ccggctttga aacgggcgac
     6361 ggttgtacgt ttcgcaaggt gccatcggga aattccgtgc gcggcagaat aatcgcaaag
     6421 atcagggaaa gagcggcggc gctcgaacgt gagacctggg ttctttaccg ggcctaccgg
     6481 gatccccggg tttcccggcc ggcccggctg ataatagccc tcgtggtcgg ttacgccttc
     6541 agcccgatcg acctgatacc cgatttcatc ccggtcctgg gttaccttga cgacctgctg
     6601 ctggttccgg ccggaatcct gctggccctc aggatgatcc cgccggaggt cctggccgaa
     6661 tgccgggccc gggtgcggga ggatttcgaa gccggacggc ggccggggcg ttgggcggcc
     6721 gtggttatcg taatcatctg gctgcttctg attgtcctgc tgttccggtg gctgagccgg
     6781 ctggcggccg gccgagccgg aggttcttcg tgaagtacct gagcgcggcc gaaatgcggg
     6841 ccctggaacg ccgggccatc gaggagcttg gcatcccggc gctccgcctg atggaacggg
     6901 ccggccaggc gctggccggg gaggccttcc ggctggccgg ggtcggtaat taccttgtca
     6961 tttgtggtta cgggaacaac ggcggtgatg gcctggcggc cggccgcctc ctggcggagc
     7021 ggggcggccg ggtccgcctt ttcctggttg gtccgccccg gccattcacg gccgagacgg
     7081 ccgccaactt cgaagcgctg aggaagaccg gcctggcggc cggccgcctc gaaaagaccg
     7141 ccgacctgga ggttttagag ccgcccgagc cgccggcggc ggtgatcgac gcgattttcg
     7201 gcatcgggct gcgcggcccc ctggaaggtt tttacgccga cctggtggaa cggatcaact
     7261 ccctcggggt gccggtggcg gccgccgacc ttcccagcgg cctggacgcc gaccggggcg
     7321 aaccgttggg atgcgcggtc cgggcctcgg tgaccgtgac cttcggccat ccgaaactgg
     7381 gtttccgcaa cccggcggcc gccgcctacc tgggccgggt ggtggtggcc gacatcggct
     7441 tgccgccgga taatctttaa acaacggcat ttgtccgaaa aacaaacttt atggtaatct
     7501 taatcaacat tattttttaa ttagtaattt ttagccgaca ttaggagccg gttatgctga
     7561 agaaacaatt ttgtcgggct gctttactgg cttttctgct gctctggtct tttgacgggg
     7621 aggcccacct ttgcgacaac gtcttccggc agcaggacaa gctgatcgtc aagcccgaga
     7681 attacaatct ggtcgtcaaa gaccggctga ccttcaagat tttcctccag aacaacatgg
     7741 accggggcat cgccgagatc agccttcagg ccgacagccc ggccttcgac tttcagatta
     7801 ccccggcccg gatgtccata cccaaggacc agcgcgcctt cttcgaggtg accatgtcgc
     7861 cccatcccgg cgtggccagc gggaattacg gggttaattt ccgcctggtc ggcggcggcc
     7921 gcgagttcaa acgcttcagc cttaacaccg gcggcgaagc ggccggcccg gcggccggaa
     7981 ccgagcccgg ccggaccgcc cccgcggagt ccaaggccag tcgctttcag gtccgggcga
     8041 tgtccacccc gccccaggtg gacggtcagc ccggcgaccc ggcctggaag ggggcggcgg
     8101 tcgcctcgaa attttcctcc cgggcgggcg gcaacgccgt caaccagacc tacggcctgc
     8161 tggggtacga ctccagccgg ttttacctgg ccgttttctg ccgggacgaa cggcccgagc
     8221 agatcggcct tcgggaccag gtccggatct ggctggccgg tccggccggc gccccggttt
     8281 acggggtgac catctcggcg gccggttccc agacgaccag ccggatcgag gcctccggga
     8341 agcagtcggc ctggagtccg gccgggctga agcagtcggt ctcccgccag aaggacggct
     8401 ggctggtcga atgctccatc cccttctccg acctgaacct ttccggtccc cagctggtcc
     8461 gcccctggtt cctgcgcctg gaacgattca aggcttccgg tgtctcggaa gagagctact
     8521 ggtcggccga cgcctccggc ttcaattccg agcggggtta cggtcagttc atcatggcac
     8581 cctgatcccg gagcccaacc agccgcccca tgaaacgaat cttgatcaac gccgggatcc
     8641 ttttcctgct ctgcctgtcg gccgccgggg cgtcggccgc cgaaaggctg gagatctcct
     8701 tcacctaccc ttccttcaag ggcgattttt acgcctcggg cctggtcgcc tttccgcccg
     8761 gggcggttgg cgatgagaac cagctgaagg tcgtcaatgc cgccgggctg gaggtggcga
     8821 ccaacgtcaa ggtccgggag acctggttcg acggttccct gctgagcgcc gacatcacct
     8881 ttgcggccaa ttccagccgt cccgaccgtt accatctcgt ttacggcggc gatacgctgc
     8941 gggcgaagcg cctgggcgaa accgccgttc tgccggtgtc ggctttttcg gtgggcggcg
     9001 ccgcccggac cgtcgagacc atggacatgc cggtgggcca gatcaacgtg cgggtcgacc
     9061 gcagtcccga tatccgctac tactggcacc tggttccgtt gaccgcgctc ctcttcctga
     9121 ccgtgatccg ctcccggagg acctcccggt gaaaccgcga cgccggatta ccctgagtcg
     9181 ccggattatc cagctggcct gcctgatcct gcttgtttac ggcggatacc tcttcaaggg
     9241 gcggctggcc ggccaggagg ccaacgtggg cgaaccgccc ggaaccggcg gaagtctttt
     9301 ccccgacctg aaggcgccca agggcgcgat gtcaaccacc cagttcgcgc ccgggagcat
     9361 cctctggccc tccggcgcca ccccggtgct ggagaattat ccgcccaccc tggtctgccg
     9421 tttcaacccg aagggaggcc tcttcaaggc ctgtatcgtc cacttcttct cggagaactt
     9481 cacctggcag acccacctca agtacctgct gccgcacctg accctcttcg tgctgctctg
     9541 ttttctcctg ggtcggttct ggtgcggctg ggtctgcccg atcggctccc tgggcgatct
     9601 gctgagctgg ctccggcgcc ggatgaacgt gccggtccgc cgtttcccgg ccggcttccg
     9661 gaatgcgctg gtcttctcct cctacggcct gctgggagcc accctgggca tctcgacctt
     9721 catcggcctg cccaactact cccgtttcca gtgctactgg ttcctgcctt actgccagat
     9781 ctgcccggcc cgcctggtct gcccggtctt cggcctcatc aagcccggct ggaaggattt
     9841 taccaacggg gtcagcgcca cctttaccct gctggcctgg ctggttctgg gcctcttcgt
     9901 ggcggctttc tactgggggc gccgggtctg gtgccatctc tgcccggtcg gcctggtcaa
     9961 ctcctggttc aaccgggggg ccggcctgga actgcacaag aaggcggtca agtgcaacaa
    10021 gtgcggctac tgcgccgacg cctgcccgat ggggttgacc gccatgtacg aggccgatac
    10081 cgaccggaag tacaaccaga acggctgtat catgtgcctg cgctgcctgg aggtctgccc
    10141 caaggacggc tgcctctcct gccgtttctt cggccgccgg gtgctggagt cgcgctatta
    10201 gaggaaaaag catgaagaag catcaagacc agggtggact gaagcccgag tatgcctcga
    10261 tcctggacgg tttttaccgg ggcctggcgc cttccggtct cagcccggac taccgggccg
    10321 agaccgaggt ttaccgccgt cgcagccgcc tggccagcgc cggccggttg tacgagagcc
    10381 tgatgtccgc ttccggggtt gcggccggcc cgcgggtcgg ctacttctgc aacctggtgc
    10441 cggaggagtt gatccgggcc tgtggggcgg tgccggttcg gctctgcgaa ctagatcacg
    10501 cctgcgcacg gctcggcgag gaggtgctgc cgggcgacgc ctgcccgatg atcaagtcga
    10561 tctgcggttc ggccgccgcc ggccgctacc gggacctcga cctgctggtg atcccggccg
    10621 cctgcgacgg caaggcgaaa ctatccgaac tcctggcgcc ggttcggccg gtctacctgc
    10681 tggacctgcc ccgttccagc gagtacctgc gcaacgccga tgcctggacc ggccggtacc
    10741 aggacttctt cgattttctg aaggagcgct tccggagccg ggccggccgt tccgaactga
    10801 cggccgaatg ccggcgcgcc aacctccgca cccagctttt tcgaaggctt taccacttcc
    10861 gggccggtca tcccggcctc atcaaccatt tcgactactt caccctggtc tccctttcct
    10921 tccggctgga accggaggcc tggctggacc tggccgggga gattctggcc gaggcggtcg
    10981 gccgggccga aggtctcaag tttgacggcc ggcgcatcct cctggccggg tccccgatca
    11041 tctttccggc ctacaaggtg cttgacatcc tttatgaatt caattgcgaa gcggtggccg
    11101 actccctctg ctccgggtac ggccacctct acgatccggt cgagattgac gaggagaccg
    11161 agaccgggat catccgggcg ctggccttga agtacctggc cgccagtctc tgcccctgct
    11221 tcatgggttt gaacaagctc ctggacctgc tggtcgacac cacggccgag taccggctgg
    11281 acggcgtcat ttaccacgac ctgcgcctct gccaggtctt tgacatgcag acggtcgcca
    11341 tccgacaggt tctcaaggag aagcggatcc catttctttc catcaagacc gacctcggcc
    11401 gtgaggatac cgggcagctc aagacccgga tcgaggcctt tctggagatg ctgggataaa
    11461 ccatggccat cgcggggctg gatatcgggt cggtggccac caagtgcgtg gtggttgacg
    11521 acggccgcat catcggcgcc gccctggtcc ggtccgggac cgacaccgag aaggccgcca
    11581 cccgcgccct ggccgacgcg cttcagcagg cccggctgga acgggccgtc ctggaaaaga
    11641 t
//
LOCUS       DAOQYN010000031        11632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_5995_length_11632_cov_4.307852, whole genome shotgun sequence.
ACCESSION   DAOQYN010000031 DAOQYN010000000
VERSION     DAOQYN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5995_length_11632_cov_4.307852"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /gene="aspS"
                     /locus_tag="PKN80_02315"
     CDS             complement(<1..1628)
                     /gene="aspS"
                     /locus_tag="PKN80_02315"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNQ34878.1"
                     /translation="MDYRKRTGCGKLRPADIGREVCLAGWVDARRDHGDLLFIHLRDI
                     SGIVQVVFNVQAGSLYEAAGRLKEEYCVLITGQVVRRLEGTENPALETGDIEIKAAGL
                     EIISASKTPPFPISEKAMTAGASVKGTEGVEEDLRLQYRYLDLRRPSLQKHLVNRYRI
                     IKAARDFLDSRGFIEVETPTLTRSTPEGARDYLVPSRLHPGNFYALPQSPQLFKQLLM
                     VGGLERYFQVVRCYRDEDLRPNRQPEFTQLDLEASFIDEEFIYELMEEALVRMFAVAG
                     ISLPRPFPRISYREAMETTGTDRPDLRFDLRLVEATDLFRNTGYAIFRKIIGQGGRIK
                     GLIVRGKSEILSKNVLQNEYAREIVPGFGAKGMTWMRVAGGKLESNIVQFFNPGEQQA
                     ILERFQAEDGSVIMMIADTSSETVLSALGQLRLHLARRLELIPGNKYHPVWVTGFPLF
                     EATEAGLTSKHHPFTAPDRTDFDPADRPGLLSLNSRAYDLVVNGEELGGGSIRINDAR
                     VQRRIFQALDLSEAEVEEKFGFFVRALDYGTPPHG"
     gene            1747..2634
                     /gene="cysK"
                     /locus_tag="PKN80_02320"
     CDS             1747..2634
                     /gene="cysK"
                     /locus_tag="PKN80_02320"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNQ34879.1"
                     /translation="MEVKQVLDLIGNTPLVQLRGERIFAKAEFLNPGGSIKDRVARAM
                     IEGAEKTGRLKPGALIVEPTSGNTGIAIALIGRLKGYRVRIVMPEGMSEERKKIIRAL
                     GADLVLTPDREGIAGAVETVRRMAAADPRIFVPQQFENPDNPRVHYEETARELWRQMD
                     GRIEAFVAGVGSGGTLQGVATFLREKNPKVIIAAVEPKNVSALLGHEPGLHQIQGIGD
                     GFIPAILDVSIIDRVIEVSDEDAISATRSLSSENGLLVGTSSGANVWAARLLAKSVKG
                     NIATVLPDRVERYFSTALL"
     gene            complement(2615..3211)
                     /locus_tag="PKN80_02325"
     CDS             complement(2615..3211)
                     /locus_tag="PKN80_02325"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNQ34880.1"
                     /translation="MKKSLGARTIIFPTPVLIVATYDPAGRPNAMAAAWGGICCSDPP
                     CVAVSLREATYTYGNLMARKAFTINIPSEEYLREADYLGLVSGRNVEKFPAAGLTPAP
                     GKSVDAPVIEEFPLVLECALLKSVRIGLHTQFIGEIKDVRAETAVLDAGGNPGIGLVK
                     PLIFDPAGRAYYGIGPRLGQAFAAGKSIGAPPAKEGRC"
     gene            complement(3267..4091)
                     /locus_tag="PKN80_02330"
     CDS             complement(3267..4091)
                     /locus_tag="PKN80_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ34881.1"
                     /translation="MAKRKVIRIDDKKCTGCGLCIPNCAEGALRIIDGKARLVSDLFC
                     DGLGACLGHCPEGAISIEEREAEAYDEKRVMEHVVSQGENVIRAHLEHLEAHGQDDFL
                     EQAREFLREKGIRLSGPAGKRSGPPPSGCPGMRTLDLGKKAGRKISARGAESRLSNWP
                     VQLMLVPPSAPFLKGADLLVAADCVPFACADFHDEYLAGRVLLVGCPKLDDRDFYREK
                     LTLIFKENRIASVTCLHMEVPCCSGLVRLIEEAISASGQAIPFREITIGIKGGRPV"
     gene            complement(4121..5194)
                     /locus_tag="PKN80_02335"
     CDS             complement(4121..5194)
                     /locus_tag="PKN80_02335"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNQ34882.1"
                     /translation="MDIVKLDSNMAVKEPGKDLLWYDAARLTIEGRGWPETEATYSRL
                     PARAKGVVREQIWSLGKCSSGLSIHFSSNTPSLAVRWDGNRAMTHMPATGVSGVDLYV
                     RHEGKWRWLAVGKPQKDVNQLQLFSDIGSEEREYILYLPLYNPVNKVELGLPASFELK
                     AKPPSRKKPVVFYGTSITQGGCASRPGMCYTAILGRWLEYPTINLGFSGNGIMEAEVI
                     DLLCELDPSVYVLDNMPNMQEDGIREREEAAIRKLKRARPGTPIVLIDSMLYCDAFLK
                     KSRRDRCVSSNRAQYAIYEKLLKEGLTGLYYIKDDGLIGSDGEATVDGTHFTDLGYLR
                     FSEKLFPVLRQILDGQGNTRPIK"
     gene            complement(5430..6206)
                     /locus_tag="PKN80_02340"
     CDS             complement(5430..6206)
                     /locus_tag="PKN80_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ34883.1"
                     /translation="MINSILQILGRTFVMAEMETRKIIHDPTELITRAVQPILWIGIF
                     GQALSRIRAIPTQGFTYLQFITPGILTQSVTFVAIFYGLSIIWERDMGLLQKILVTPT
                     PRLALVWGKMLSAGIRGLTQAVIILLFALVLRIQLRVSPASILGVVVITILGAGFFSS
                     LSMIIASIVKTRERFMGIGQIITLPLFFASNAIYPISIMPGWLQRIANLNPLSYMVSG
                     LRALLVTGDLSRLPLDLVVLATATILISFLGAYMYPKVVI"
     gene            complement(6211..7017)
                     /locus_tag="PKN80_02345"
     CDS             complement(6211..7017)
                     /locus_tag="PKN80_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ34884.1"
                     /translation="MTPDTIITVQGLTKRFKNLTAVNAVSFNIRPGEIFAFLGPNGAG
                     KTTTIKMLITLLAPTSGDAFIGGHSITRQPARVREIIGYVPQMISVDGALTSRENLVL
                     MSRLYDVPRCERKERVEEMLAFLKLEKYADSLVRTLSGGMIRKLEVGQAMLHHPHLLF
                     LDEPTTGLDPVAKRNVWEHLLELRNTLGTTIFFSTHNMEEAEDVCDRVAIMNLGRIAA
                     IGTVPGLKKETGKAGATLEDAFIFFTGNRLQEGGNFRDIRRTRQTQRRLG"
     gene            complement(7345..7557)
                     /locus_tag="PKN80_02350"
     CDS             complement(7345..7557)
                     /locus_tag="PKN80_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34885.1"
                     /translation="MDWYARKFERFMQLPRPLMILHVSSKTLFGLGAGILWGMAAGTA
                     AKTLGWSIIILSLLVAIPSSCRILRR"
     gene            complement(7589..7726)
                     /locus_tag="PKN80_02355"
     CDS             complement(7589..7726)
                     /locus_tag="PKN80_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34886.1"
                     /translation="MARSHFTMRQKNISRLLRERKVILREKLKTSRRVGKRSGAGAKS
                     G"
     gene            complement(7746..8261)
                     /locus_tag="PKN80_02360"
     CDS             complement(7746..8261)
                     /locus_tag="PKN80_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNQ34887.1"
                     /translation="MKYLISGILFLLLFAGDASALEIKSRAFRNGTPIPTRHTGDSLN
                     VSPPLAWKGAPAGTRSFALICDDPDAPGRIFVHWVIYNIPAEKSDLEEDVSKKERLTD
                     GTLQGRNDTGRIGYLGPAPPPGKPHRYFFKLYALDIQLKLKPGAKKAGLEEAMRGHVL
                     AEASLMGTYQR"
     gene            8399..9208
                     /locus_tag="PKN80_02365"
     CDS             8399..9208
                     /locus_tag="PKN80_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34888.1"
                     /translation="MKTFKRILVVYSEKISSGHRQAVEGVRRRLQGRRYRMVRSQRLC
                     GRDFDRVDLVITVGGDGAFIRAAAFVERAAILGINSEPEYSEGALTSIRDDELDRLNE
                     FLAGRFATITRQRARAELNGQPLPSSALNEIYVGARYQFHTSRYLIEYRGVRESQRSS
                     GVLVVTGSGSRAWYHSSGGRPFHYSERRLRFLVREPYSGRLFRPRLLHGEVGPADRIR
                     FESQRQAGGIVVLDAWRTYPFNRGSRLEISLSEWPLTVLVPTGSPSRAGLS"
     gene            9320..9928
                     /locus_tag="PKN80_02370"
     CDS             9320..9928
                     /locus_tag="PKN80_02370"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNQ34889.1"
                     /translation="MKKMKTGGLEQALEKAVCALASTIEYRDPFTAAHQVQVTRLACR
                     LAKELNLSAERTTGLRVAGIIHDLGKVYLPVEIASKPCPLTPAEMEIVMGHPQLGCDI
                     IKEVKFPWPVQKIILQHHERLDGSGYPRGLKGRAIILESRIMAVADVMQAIVSQRPYR
                     PGLGLNRAREVIIEGRGARYDPEVVDACLDLFKRKRFSFDRP"
     gene            complement(9983..10654)
                     /locus_tag="PKN80_02375"
     CDS             complement(9983..10654)
                     /locus_tag="PKN80_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34890.1"
                     /translation="MEIEPFLRFLSFAVFFLIFFITYYRRTTRVVRARFNSLAASLNG
                     RVKSVLGLNFSLGADYRGLPIQITYSPGDNNRPPSLAIAARRRPPFRFRLQKQDWNTR
                     LSSRLGLARGFKSTDPDFDQRFFASTGDRNRCAVFLADVRNRSELEALAEAGWQVEFG
                     RRQALIRKKLTRTPIVFREELRKRNLRNAAREIFGLSNAAADFSETELLQVLERFERL
                     LRNLM"
     gene            complement(11049..>11632)
                     /locus_tag="PKN80_02380"
     CDS             complement(11049..>11632)
                     /locus_tag="PKN80_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34891.1"
                     /translation="GAFERLKENSIRLGAAAGERATPEVFRSGYGNHFLKAGVSDLYA
                     LVKITRADQTLVVRARVEDDVNQPLRDREPGLKSSGDHLRLAFQGSDGRVRFLLIAPT
                     PQGGLIRLLDGGAGRSLPGTARWTGNTWEAEVRVPMKDLGLPETLEAPVLFDANVIDS
                     DSSAEMVDGELAWSGAAFETGETKGFGWLRFQK"
BASE COUNT         2121 a         3446 c         3725 g         2340 t
ORIGIN      
        1 ccgtgcgggg gcgttccgta gtcaagggcc cggacaaaga agccgaactt ctcctccacc
       61 tccgcctcgg agaggtcgag ggcctggaag attcggcgct ggacccgggc gtcattaatg
      121 cggatgctgc cgccgcccag ctcttcgccg ttgacgacca ggtcataggc gcgcgaattg
      181 agggagagca gtcccgggcg gtcggcgggg tcgaaatcgg tccggtccgg ggcggtgaag
      241 gggtggtgct tggaggtgag gccggcctcg gttgcctcga agagcggaaa gccggtcacc
      301 cagaccggat ggtatttgtt gccgggaatt agttccaaac gccgggccag gtgcagacgg
      361 agctggccga gggccgaaag gaccgtttcg gaagaggtat cggcaatcat catgatcacg
      421 ctgccgtcct cggcctgaaa ccgttccaga atggcctgct gctcgccggg gttgaagaac
      481 tggacgatgt ttgattccag cttgccaccg gccacccgca tccaggtcat gcccttggct
      541 ccaaagccgg gtacgatctc cctggcgtat tcattctgga ggacattctt gctcaggatc
      601 tcggatttgc cccggacgat gagccccttg atccggccgc cctggccgat gatctttcgg
      661 aagatggcgt aaccggtatt ccggaagagg tcggtggcct cgaccaggcg caggtcgaag
      721 cggaggtcgg gacggtcggt gccggtcgtc tccatggcct cccggtaact gatccggggg
      781 aaaggccggg gcaggcttat tccggccacc gcgaacatcc ggacgagggc ctcctccatc
      841 agttcgtaga tgaactcctc gtctatgaag gaggcctcca ggtccagctg ggtgaactcg
      901 ggctgccggt tggggcgcag gtcctcgtcc cggtaacacc ggaccacctg gaagtagcgt
      961 tcgaggccgc caaccatcag cagctgcttg aataattggg gtgactgggg aagcgcgtag
     1021 aagttcccgg ggtggaggcg gctgggtacc aggtagtcgc gggccccttc cggggtgctg
     1081 cgggtgaggg tgggcgtctc cacctcgatg aagccccggg aatcaagaaa atcccgggcg
     1141 gccttgataa tccggtaacg gttgaccagg tgcttttgca gggaggggcg ccgcaggtcc
     1201 aggtagcggt actggagacg caggtcctcc tcaacgcctt cggtcccctt aacggaagcg
     1261 ccggcggtca tggccttctc cgagatgggg aaggggggcg tcttcgaagc gctgattatt
     1321 tccaggccgg cggccttgat ttcgatgtcg ccggtctcca gggcgggatt ctcggtaccc
     1381 tccaggcgcc ggaccacctg gccggtaatc agaacgcagt actcctcctt cagccgtccg
     1441 gcggcctcat aaaggctgcc cgcctggacg ttgaagacca cctgcacaat gccggagatg
     1501 tcccgcaggt ggatgaacag gaggtcgccg tggtcgcgcc gggcgtcaac ccagccggcc
     1561 aggcacactt cgcgcccgat atcggcaggg cgaagttttc cgcagccggt cctttttcga
     1621 tagtccatgt ttttgtttcc ggatcgggat ttgagtcaag aatagtcttg attttatcct
     1681 cattcccggt tgccggtcaa accggcctgg gttatactat tggagaaccg gagaggagcc
     1741 taacttatgg aagtgaaaca ggtgcttgac ctgatcggaa acacgccgct ggtccagctg
     1801 cgcggcgaac gcatctttgc caaggccgaa tttctcaacc ccggcggcag cataaaggac
     1861 cgggtggccc gggccatgat cgagggggcc gaaaagaccg gccgcctcaa gcccggcgcc
     1921 ctgatcgtcg aaccgacctc cggcaatacc gggatcgcga tcgccttgat cgggcgtctc
     1981 aagggatacc gggtccggat cgtcatgccg gagggcatga gcgaggagcg caagaaaatc
     2041 atccgggccc tgggggcgga cctggttctg accccggacc gggaaggtat cgccggcgcc
     2101 gtggagaccg tccggcggat ggcggcggcc gatccgcgca ttttcgtgcc ccagcagttt
     2161 gagaatcccg acaacccccg ggttcattac gaggagaccg cgcgcgaact ctggcgccag
     2221 atggacgggc ggatcgaggc tttcgtggcc ggcgtcggca gtggcggaac cctgcagggc
     2281 gtggctactt tcctcaggga gaagaatccg aaggtgatca tcgccgcggt ggaaccgaag
     2341 aacgtttccg ccctgctcgg ccacgagccg ggcctgcacc agattcaggg gatcggcgac
     2401 ggtttcattc cggccatact cgatgtttcg atcatcgacc gggtcatcga ggtctcggac
     2461 gaggatgcca tttccgccac ccgctccctg agttccgaga acggcctgct ggtggggacc
     2521 tcttcgggcg ccaacgtctg ggcggcccgc ctgctggcca aaagtgtgaa aggcaatatc
     2581 gccacggtgc tgcccgaccg ggtcgagcgt tacttcagca ccgcccttct ttagccgggg
     2641 gagcaccaat cgacttcccg gccgcgaagg cttggccgag tcgcggtccg attccgtagt
     2701 aggcccggcc ggccgggtcg aagatcagcg gtttcaccag gccgattccg ggatttcccc
     2761 cggcatccag caccgccgtt tcagcccgga cgtccttgat ctccccgatg aactgggtgt
     2821 ggagaccgat tcggaccgat ttcagcaggg cgcactccag gaccagcgga aactcctcga
     2881 tcaccggagc gtcaaccgat tttccaggtg ccggggtcag cccggccgcc gggaacttct
     2941 ccacgttccg gcccgaaacc aggccaaggt aatcagcctc ccgcaggtac tcctccgagg
     3001 gaatgttgat cgtaaaggcc tttctcgcca tgaggttacc ataggtgtag gtggcttctc
     3061 tcagggagac ggcgacgcag ggcgggtcgg aacagcagat gcctccccag gcggccgcca
     3121 tggcgttggg gcggccggcc gggtcgtagg tggcgacgat aagaaccggt gtcgggaaaa
     3181 ttatggtccg ggctccgagc gattttttca tgtcggcctc cgctggcggt tgtaaaatcg
     3241 aacggcgcga cgggagcggg tcccggtcag acagggcggc cgcccttgat tccgatggtg
     3301 atctcccgga acgggatcgc ctgcccggag gcggaaatgg cctcctcaat gagcctgacc
     3361 aggccggaac aacagggaac ttccatgtgc aggcaggtta ccgaggcaat ccggttctcc
     3421 ttgaatatca gggtgagttt ttcccggtaa aaatcgcggt cgtcgagctt cggacagccg
     3481 accagcagga cccgtccggc caggtattcg tcatgaaaat ccgcgcaggc gaacggtacg
     3541 cagtcggcgg cgacgagcag gtcggctccc ttgaggaaag gggccgaggg cggcaccagc
     3601 atcaactgga ccggccagtt gcttaatcgt gattccgcgc cccgggcgga aatcttccgg
     3661 ccggccttct tcccgagatc gagcgtccgc atcccgggac aaccggacgg aggcggcccg
     3721 ctccgtttcc cggccggccc ggagaggcgg attccttttt cccgcaggaa ctcccgggcc
     3781 tgctccagga aatcgtcctg gccgtgcgcc tcgaggtgtt ccaggtgggc cctgattacg
     3841 ttctcgccct ggctgacgac gtgttccatc acgcgctttt cgtcgtatgc ctcggcctcc
     3901 cgctcctcga tggagatcgc tccctccggg cagtggccca ggcaggcgcc cagcccgtcg
     3961 cagaagagat cgctgacaag ccgcgccttg ccgtcgatga tccgcagagc gccctccgcg
     4021 caattgggta tgcagaggcc gcagccggta cacttcttat cgtcgatccg gatgaccttt
     4081 ctcttagcca ttttcaaggc ccgtttttta ggagacgttt ttacttgatc gggcgggtat
     4141 ttccctggcc gtccaggatc tgcctgagga ccgggaaaag cttctccgag aagcgcaggt
     4201 agcccaggtc ggtgaagtgg gtgccgtcga ccgtagcctc gccgtcgctt ccgatcagac
     4261 cgtcgtcctt gatatagtaa aggccggtca ggccctcctt cagcagtttt tcgtaaatgg
     4321 cgtactgggc ccggttggag gagacgcagc ggtcgcgccg ggacttcttg agaaaggcgt
     4381 cgcagtaaag catgctgtca atcagtacga tcggagtccc gggccgggcc cgcttcagct
     4441 tccggatggc cgcctcctcc cgctccctga tgccgtcctc ctgcatgttg ggcatgttgt
     4501 ccagcacgta gaccgacggg tccagctcgc agagaaggtc aatgacctcc gcttccatga
     4561 tgccgttccc ggagaagccg aggttgatgg tcggatactc cagccagcgg cccaggatgg
     4621 ccgtgtagca catgcccggc ctcgaggcac agccgccctg ggtgatcgag gtgccgtaga
     4681 agacgaccgg tttcttccgg ctcggcggct tcgccttaag ctcgaaggag gcgggcaggc
     4741 ccagttcgac cttgttgacc gggttgtaaa ggggcaggta gagaatatac tcccgctcct
     4801 cgctgccgat gtccgaaaag agctggagct ggttgacatc cttctggggt ttgccgaccg
     4861 ccagccacct ccactttccc tcgtgccgga cgtaaaggtc gacgccgctg acgccggtgg
     4921 ccggcatgtg ggtcatggcc cggttgccat cccagcgcac cgccagggac ggggtgttgc
     4981 tcgaaaaatg gatgctgagg ccggacgaac actttcccag cgaccagatc tgttcccgga
     5041 ccacgccttt ggcccgggcc ggaaggcggc tgtaagtggc ttcggtctcc ggccagcccc
     5101 tgccttcgat ggtaagccgg gcggcgtcat accagagaag atccttgccc ggttccttga
     5161 ccgccatgtt gctgtcgagt ttcacgatgt ccattttcgt ttccgattgg gctaaaagaa
     5221 aaggccggtt ctcaccggcg gcgacgccgg aaaaaagcag caggataaga gagctaaccg
     5281 ccagtcggtg cctcaaggta acctccttcg gatcgggccg tcttccggtt gaaaatcagc
     5341 ctgtaatttt accctgcggc gggcgagcgg tcaaggttaa tcgtaattcc ggccggcgga
     5401 atttccggcg ggctcttttc tggcccgact cagataacca ccttgggata catgtaggcg
     5461 cccaggaagg agatgagaat cgtggccgtg gccaggacga cgaggtccag gggaagccgc
     5521 gagaggtccc cggtgaccag gagggcgcgg aggccgctca ccatgtagct gagcgggttc
     5581 aggttggcga ttctttgcag ccacccgggc atgatcgaga tcgggtagat ggcgttggag
     5641 gcgaagaaaa gcggcagggt gataatctgc ccgattccca tgaagcgttc gcgggttttt
     5701 acgatcgagg cgatgatcat cgaaaggctg ctgaagaagc cggcgccgag tatcgtgatg
     5761 acgacgactc cgaggatcga agccggcgaa acccggagct ggatccgcag aacgagggcg
     5821 aagagcagga tgatgaccgc ctgggtcaat ccgcggatgc cggccgagag catcttgccc
     5881 cagaccaggg cgagccgggg cgtcggggtg accaggatct tctgaagcag tcccatgtcc
     5941 ctttcccaga tgatggaaag gccatagaag atggcgacga aggtcaccga ctgggtaagg
     6001 atgccggggg tgatgaactg caggtaggtg aagccctggg tcgggatggc ccggatccgg
     6061 ctcagcgcct ggccgaagat gcctatccag agaatcggct ggacggcccg ggtgatgagt
     6121 tcagtcgggt cgtggatgat cttgcgggtc tccatctcgg ccatcacgaa ggtccggccg
     6181 agaatctgca ggatgctgtt tatcatgaac tcaccccagt cttctttgcg tttgccgcgt
     6241 ccggcgtatg tcacggaagt tgcctccttc ctggaggcgg ttgccggtaa aaaagatgaa
     6301 agcgtcttcc agggtggcgc cggccttgcc ggtctctttc ttcaacccgg gcaccgtacc
     6361 gatggcggca atccggccga ggttcatgat cgcgacccgg tcgcagacgt cctcggcttc
     6421 ttccatgttg tgagtggaga agaagatggt cgtgcctaag gtgttgcgga gttcgagaag
     6481 gtgttcccag acattccgct tggcgaccgg atcgaggccg gtggtcggtt cgtcgagaaa
     6541 gagcaggtga gggtggtgaa gcatggcctg cccgacttcc agtttccgga tcatgccgcc
     6601 ggagagcgtc ctgaccagcg aatcagcgta tttctccagt ttcagaaagg cgagcatctc
     6661 ttcaacgcgt tccttccgtt cgcaccgggg aacatcgtag agccgggaca tcaagactag
     6721 gttttcccgg ctggtgaggg ctccgtcaac cgagatcatc tgcggcacgt atccgatgat
     6781 ttcccgaacc cgggccggct ggcgggttat gctgtggccg ccgatgaagg cgtcgccgct
     6841 ggtgggcgcc agcagggtga tcaacatctt gatggtggta gttttgccgg cgccgttcgg
     6901 gccgagaaag gcgaagattt ccccgggccg gatattgaag gagacggcgt ttacggcggt
     6961 gagatttttg aaccgcttgg taagaccctg cacggttatg atcgtatccg gcgtcatctg
     7021 caattttcct ggttgaacgg cctccgacgg aatgcttgaa agagaggggt ctgggcgccc
     7081 cggggaattc tttccggggc tgattgtctg cggccgggag gcttggtttt ccagccgcgc
     7141 ctgattgtct tcgcgggaga cctcggacct tgcctctcga taaagaggcc tccggtccgg
     7201 gccgaccggg tgacatccgt taaagttaaa tgttagccgc cgtggacttc acgggtcaag
     7261 ttccatccgc tggccggcgc ggaatcgccg gattccgggt cccggctggc acccggctga
     7321 agcccgcgtt tgcgatcggg ccttttaccg cctgagtatc cggcagctgc tgggaatggc
     7381 aaccaggagc gagagaatga tgatcgacca acccagcgtt ttcgcggccg ttccggccgc
     7441 catgccccag agaatcccgg ctcccagtcc aaacagggtc ttgctggaaa catggaggat
     7501 catcaggggc ctgggcagct gcataaaccg ctcgaatttc cgggcgtacc agtccatggt
     7561 tgcctccttg cgcgtggcgg ccggattttt atccggattt cgctccggcc ccgcttcttt
     7621 ttccgacccg ccgggaagtc ttcaatttct cccgcaggat gaccttcctt tcccgcagca
     7681 gccgggagat gttcttctgc ctcatggtga agtgcgacct ggccatttcc ggttcctccg
     7741 attcttcagc gctgataggt tcccatgagg ctcgcctcgg ccaggacgtg tccgcgcatc
     7801 gcctcttcca ggccggcctt cttcgcgccg ggctttagct taagttgaat atcgagggcg
     7861 taaagcttga agaagtaacg atgcggtttg ccgggcggcg gcgccggccc caggtacccg
     7921 atccggcccg tgtcgttccg tccctggagg gtcccgtcgg tcagcctctc ttttttggaa
     7981 acgtcctcct cgaggtcgga cttctcggcc gggatattgt agatcaccca gtgaacgaag
     8041 attcgcccgg gcgcgtccgg gtcatcgcag atcagcgcga agctgcgggt tccggccggc
     8101 gcgcctttcc aggccagggg aggggagacg ttcagggaat ccccggtgtg ccgggttggg
     8161 atgggtgttc cattacgaaa ggcccggctt ttgatttcaa gggccgaggc gtctccggcg
     8221 aagagaagca ggaaaagaat gccgcttatc aggtatttca tttcgacctc cttgctgccc
     8281 ctcggatatt ttaccccccg ggaaaagcag aatcaagcgc cttacctggc ggtagaccgg
     8341 accctctggc ccgcttttgc ctcgccggag gcggcagtgg tatgctatgg aataaaagat
     8401 gaagaccttc aagcgcatac tggtggttta cagcgaaaaa atcagttccg gtcaccgcca
     8461 ggctgttgag ggagtgcgcc gccggcttca ggggcggcga taccggatgg tacgttccca
     8521 acgcctttgc ggccgggact tcgaccgggt tgacctggtg atcaccgtcg gcggagatgg
     8581 cgcttttatc cgggcggccg cctttgtcga gcgggcggcc atactcggta tcaactccga
     8641 accggagtac agcgaagggg ccctgaccag catccgggat gacgaactgg accggttaaa
     8701 cgaatttctg gccggccgat ttgcaaccat tacccgccag cgggcccggg ccgagctcaa
     8761 cggccagcct ttgccttcgt cggccctgaa cgagatctac gtcggagccc gttaccagtt
     8821 ccacacctcg cgctatctga tcgagtaccg gggcgtgcgc gaatcgcaac gcagttccgg
     8881 agttctggtg gtcaccggct ccggttcccg ggcctggtac cactcttccg gaggccgtcc
     8941 ctttcattac tcggagcgcc gattgcgttt cctggtccgg gaaccatatt ccgggcgcct
     9001 cttcagaccc cggctgctgc acggggaggt tggtccggcc gataggatcc gttttgaaag
     9061 ccagcgtcag gccggcggca tcgtggttct cgacgcctgg cgaacctatc cgttcaaccg
     9121 gggcagccgt ctggagataa gcctctccga atggccgctt acggtgctgg ttccaaccgg
     9181 aagtccgtcc cgagccggcc tttcctgacc aattctttca acgctcggcc cggctggttt
     9241 tcccggcctt gaaaaagacc tttttgaagg gtaaaatcat tggtatgatt aaattcccgg
     9301 ccgggtgata ggaggcggca tgaagaagat gaagacgggc ggtctggaac aggcgctgga
     9361 aaaggccgtt tgcgcgttgg cctcgacgat tgaatatcgc gatcctttta ccgcggccca
     9421 ccaggtccag gtgacccggc tggcctgccg gctggccaaa gaacttaacc tgtcggccga
     9481 acgcacgacc ggtctgcggg tggccggtat catacatgac ctgggcaagg tttatttgcc
     9541 ggtcgagatc gcttccaagc cctgcccgct gaccccggcc gagatggaaa tcgtgatggg
     9601 ccacccccag ctggggtgcg atatcatcaa ggaggttaaa ttcccctggc cggttcagaa
     9661 gataatcctg cagcatcacg aacgccttga tggttccggc tatccgcggg gattgaaggg
     9721 gcgggcgatt atcctcgaat cccgcatcat ggccgtggcc gatgtcatgc aggcgatcgt
     9781 ctctcagcgc ccctatcgtc cgggactggg tttgaatcgg gcgcgggaag tgattatcga
     9841 gggacggggc gcgaggtacg accccgaagt ggtggacgcc tgcctggact tgtttaagag
     9901 aaaaagattc agtttcgacc gtccttgacc gaccgctccg gtccggccct cggaaggttg
     9961 agccggacct tttttaatct tgttacatca ggttccggag caaccgttca aatctctcca
    10021 ggacctggag cagttcggtt tcggaaaaat cggcggccgc gttcgaaagg ccgaagatct
    10081 ccctcgccgc gttccgcaag ttcctctttc gtaattcctc gcgaaaaacg atcggcgtcc
    10141 gggtaagctt ctttctgatc aaggcctgcc ggcggccgaa ttccacctgc cagccggcct
    10201 ccgcgagcgc ctccagttcg ctgcggttcc ggacatcggc caggaagact gcgcatcggt
    10261 ttcggtcgcc ggtactggcg aagaaacgct gatcaaaatc gggatcggtg cttttaaaac
    10321 cgcgggccag gccaagacga gaactgagtc gcgtattcca atcctgtttc tgcaagcgga
    10381 accggaacgg cggccggcgc cgggcggcga tggccaggct cggcggccgg ttgttgtcgc
    10441 cggggctgta agtgatttgg atcggcagcc cccggtaatc ggctcccagg ctgaagttta
    10501 accccagcac gcttttgacc cgcccgttca ggctggcggc caggctgttg aaacgggcgc
    10561 gcaccacccg ggtggtgcgt cggtagtaag tgatgaagaa gatcaggaag aagacggcga
    10621 aactcagaaa acgcaggaag ggttcgattt ccatatccgg ggaggtgtct gaaagaaggc
    10681 ggcgggccgc ccgggttttt tggggatagc gatggatgga gaaaagaatt taaacagctt
    10741 ttacaccggg gttgctatcc agggacaaaa aaccccattg agaaaaaccg gggggaatta
    10801 aggaaaagat tgccaggcgc cgctcttccc ggtttttccc tggtgatcag gatcggttgc
    10861 cggtcaactt acttcttctt gtgcgtggcg cagaacttcg acttcccgac cgcgttgttc
    10921 ttgcacttct tcccgtcgac ggtcttgccc gcgcacttcg cctttttggc agctgccatt
    10981 tgaatcaccc cctttcttct gcgtttatca tatcacatcc ggtcccgatt gcaaattccc
    11041 ggacagaact atttctggaa ccggagccat ccgaatccct tggtctcgcc ggtttcgaag
    11101 gcggcgccgg accaggctag ttcgccatcg accatttcgg ccgaactgtc ggaatcgatt
    11161 acgtttgcat cgaagagcac cggcgcctcc aaagtctccg gcaatcccag gtccttcatc
    11221 gggacgcgga cttccgcctc ccaggtgttg ccggtccagc gggccgtgcc cggcaggctt
    11281 cgcccggccc ctccgtcaag cagcctgatc aggccgccct ggggagtcgg ggcgatcagc
    11341 agaaaacgga cgcggccgtc acttccctgg aaggcgagcc ggaggtggtc gccgctggac
    11401 ttgagtccgg gttcccggtc ccggagcggc tggttgacgt cgtcctcaac ccgggcccgg
    11461 accaccaggg tttgatcggc ccgggtgatc tttaccagtg cgtagagatc gctgaccccg
    11521 gccttgagga aatggttgcc atagccgctt cgaaagacct cgggagtcgc ccgttctccg
    11581 gccgcggcgc ccagtctgat tgaattttcc ttcagtcgct cgaaggcgcc gg
//
LOCUS       DAOQYN010000032        11551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_6044_length_11551_cov_5.050626, whole genome shotgun sequence.
ACCESSION   DAOQYN010000032 DAOQYN010000000
VERSION     DAOQYN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6044_length_11551_cov_5.050626"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKN80_02385"
     CDS             <1..281
                     /locus_tag="PKN80_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNQ34892.1"
                     /translation="KYHEVLVFQVLDPDELELPFSGPHTFLDLETGDRLETDAPAIRA
                     EYRKLVGDFLETYRKGCAEHNISYVRALTNDPFDHFLHRYLERRARYS"
     gene            278..2287
                     /locus_tag="PKN80_02390"
     CDS             278..2287
                     /locus_tag="PKN80_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNQ34893.1"
                     /translation="MNFLNPLWLWALTGAALPILVHLFARKRGKVLKFSTLQFLRLSQ
                     LRTGRRRRLEEILVLLLRVLALACLLAALAGPVSRSRLFGERETAAVLILDDSLSMAA
                     AVNGRTASVRARELARSALASLPEKSEVAVLTFAGRRLPFTRRRDDVGRWLDGIEPGW
                     TAGNLPAALEEAFGLLDRKTGNRIIYLFTDGQAYPWRNVVRSAKDGRRPALVLALAGP
                     PAAGNLTCREVEPVPGRRRARCTVSNWGDRPAVAEVSLAGPAWRLARSLEVAAGESRS
                     VDFDLPEEAGRLEGGVAAAGDPLAADNVCRVDLNASGPRRIMIAGSSGPALFYLEKAL
                     ALDAETTGLELFSRESAGLSATALAGCGMVILADPGRLAPAVSAFLQDYVRSGGNLLY
                     FAGDRVLAADFNRDWQAPGGTEFLMPARIIDWPRLAVPVRIAWAAAEHPLFLPFGSRA
                     LEYLKSVNFNRLVRLEPVDGTVLMRGESGLPLLLEKRVGRGRIMLAAFLPGEDWTNLV
                     FKPGFPVLMQHLARYLSGGNLLAVRTGEVFRPLAGGGRLFSPEGSEVPAEPAAGGWRL
                     DRPGFWRLVPTASGTARERVISVNPDPEEGNLARIGAAEVRQRLAGVTVEFLEAEQAL
                     PFLERGAAARDLSDTLLRLALLLILAEAGLAAFFYHLKHREGRHA"
     gene            2280..4382
                     /locus_tag="PKN80_02395"
     CDS             2280..4382
                     /locus_tag="PKN80_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34894.1"
                     /translation="MLKLGWQSLVPGWWLILMLAGVSALIFYAYRPVHFSGRLRRWLP
                     VLHALAVVLLVLLLVQPELALGLRRRTKNRLHILVDTSLSMGIKDRRGVSRLDRAREF
                     LKARHYFRSMRPVFHTAGQELTEVSREALAGLKPEAGASRIAAAVRDLAERVGRDGAA
                     ILVLTDGADPTPPDWEGLKKNLRTPVYAVGIGESEFKDLSLAELVVNSPVYQGQTVHI
                     GAYVRQSGYPPGLARIRLKQADRVLQESTLRDSGLFSYAEFQVPAAAAGVFEYQVEVA
                     RQPNEALTDNNTGLAVVQVVAPRIKILYLDGGLRWEYKFLKRYLESDAQLEPYFLVRV
                     GGSLFQQTGGGKLSLPADFLSRRDFLKGFNLVILGDLDFTAFTGRQLAALRDFVLEDG
                     GGLALLGGDNFLRGLAGTPLEALLPVALAGGESRVLTEAFSPAAAAVSQPVTFLEGLG
                     RLPRLERRNPFRVPARGGLVLLTRDGDPGQPVAAVNLAPQGRCLVVGTDDTWRWYFSG
                     ASERQRYEEFWGRLIRYLCGPEEYLGIGRRLPDLLLDRREYGLGEPVAVRLAWRPGGP
                     EGSPEVFWQDQSGRRERLVFKSEAASFTPPAEGLYLVGATAAGRSVFKWVTVSRSGSE
                     WSEPGRNDAWLERLAETGGGRYLRLESAGGFGPELARGEPVTYNVSLARPARRFLLPA
                     VFLMLNLCWFLRRRQGVI"
     gene            4400..7159
                     /locus_tag="PKN80_02400"
     CDS             4400..7159
                     /locus_tag="PKN80_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34895.1"
                     /translation="MEEPRVLIQAYLQHYRRLVQRRRSAETGWLAGLWLAGALLFILA
                     FEYFFGLVTRAAWLSRGLLAAPLAVLLVQAFLERRRLERLEADRLALELERKFPRFGN
                     HLINAYQLGTRPKGYPAPFVNRLLGEASGMISIRELDDLPVRPRVSWYRKGLLAAAAG
                     ILLLAAAATGPIRSGFLRLLVPWAGPGTWGIRVEPGDCLVVRGSDLEIRALAAGAGAP
                     RVEFHDGPGGAAAMEPGPAGWYAHRFLAVREPFTYRVRAGRSLTPWFRVRVQDPTFLK
                     RLRINCRYPAYTGLAAAGFEQEPGDLTFPAGTRLTLEAVFNNPVGPAWLVLGTRRLGR
                     SGGRPAASRRFELTVESATLYEFHFYDYLARDYRVSPRFRLQPGFDQAPVLEFTVPAA
                     DLSASAGASIRLEVKATDDYGLRKIRFRLRSEGNDRPEADPVFFQAQLSGRREGTASA
                     YLKLPAGFSEPIAYYAEALDNHPAAPNLGRSSLYHIYPAAGRSDPSGRRGREEDGNRP
                     PEIEILKELAENLKQFAEEQKKLLEAAKRLDKPQDLRAAEKAADLAEVERKWAELFQK
                     MVDDLNKINQQTKGKFTLAQELVEMVSHLQKAAEALPHKPVHMVIPESMAGLELAEEL
                     TANLERWMAEEPDYLKWDMAEPSKRVEAPEAELPDELMDIVGDLLEQEEDMKEEIEDI
                     TSSWMDSMDKGAGWTAMDGPISNMSAKGITGNLMPNQHEVGGRAGEGRTGRSYGEMVE
                     ETAVGKGGRRTPARLTPDNREPGSIKDSSGENPLGPTGGGKSSGWGAEGLQGPVQDLN
                     FRYDQLAGRQARLVEEAEKLERKLQVLNVSNPQLEQALSSMRNFQVQLSSGRYEDLLT
                     TKQLIISNLQQVQQSIVGQTVVRLEGSERSVRRNRELRSAWEDRVPAGYEGLVQRYYR
                     DISGH"
     gene            7171..8097
                     /locus_tag="PKN80_02405"
     CDS             7171..8097
                     /locus_tag="PKN80_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34896.1"
                     /translation="MRNLTWLPALFLLAGLAASAEAAGGLNLFLNSPRAVYAEEEEVR
                     LDLMIEAEADRSVSFQYLQPKEDGFLFVGGEWRLEVRVNGVTVPEFTNHMPPEPKAGT
                     LQLNLSPGASFSARIPFYYYYYPFELPADFEVRLHYDGSVSNPVRFQVRRTEGRPVAG
                     GWLINGDFRSGRDFPLGWRLTGDRVRWEKEPPALRLTLDRATAEGPGLWVYSTFQELK
                     APAEFTLKVRARSQGQEIIVFVEGWGLVAGRRRLLERNECFMHPAGTESADYRTAAVF
                     RNPQVRWLRVKVYTYLKVGDIRFESVDLVPKE"
     gene            8101..10158
                     /locus_tag="PKN80_02410"
     CDS             8101..10158
                     /locus_tag="PKN80_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ34897.1"
                     /translation="MERLKNSIIRPAAPLLWAATVFLMLAAAAGTASSQADWPEGWEY
                     RMALEVDSGRLDAAGDRLAILDFCGAARPDGADLRLFDESGSSVPFQLLQAGPGQRYR
                     ILFPASRGRCRLYFGNPAAAAPAPADFPIRRGLWLEVYERKGDRVDTWEEARAVAEAS
                     RRTGRRLGRAAWKQVWDGANPFGPEKDVVKIYTGYFRSEAGVYEFATSSGGASFLLID
                     GQLTAAWPGWHGAEPFIRPARTGKVTLTAGIHELAYYQFSRSGQEITAAAVKRPGEKD
                     FQVIPADFFLPLAGAIPVNLERRGKPVAAALTWENTHYLRRENWELLTFRFFDLSHPD
                     SEIEVRQWDFGDGQSAVGRNPLHTYLKPGLYPVTLTVRGRRGAGSDRVRLTVPVEQNY
                     GEMRLPSRSLEAYLEEFDRLDLDRLGNEELGILAQLYQSYDRSEAAYRCYRNLAGRRL
                     SSEERRRAGAVAAALAVRERDYAGAAELYRQLLAERPAPEFLVKLGWVQLRLGELDQA
                     DTLFRQAETAAGARPEERRLATIGRGDVLRQRGEAEAARQAYLPLTERGNLESRNGWY
                     DQQVLYYVRQKDFFTAWERLEAWGEELPLVKLEGAWSILAARIGLERKDYDGARQELE
                     TFLKVTDSEAGNAYRPGAVYLLGQVWEAQGERARARGFYQQVVDRYPGTSWAGLAADR
                     IKK"
     gene            10187..>11551
                     /locus_tag="PKN80_02415"
     CDS             10187..>11551
                     /locus_tag="PKN80_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34898.1"
                     /translation="MKKKGTGTQGKPLPYLRLESIGRAGWLRADRRLERIGGWSDLAA
                     VQAELNRIFEEIFGPRPDPAAPVPFRELAGFSFAGVEIRQVLLEPEPGYRISGFTFRS
                     GSGSGPGPALVVASGHVERAALYLPYLKLCLYAAQNGFYVLAFDPVGQGSRREVFLPG
                     ADPEYWSPCDQHQDLAAVAMLTGRQLAHYLVRDNTSAISFVASRPEVDPERIGFTGQS
                     GGGIQTFLALADSRVRAAVPMQATSSRRGAFQHLVMRDMDQIFWHCWERGFDQSELVA
                     LFAPRPVRIIAEHGYPDQVDIYRRLEPVYRKLGLPEQLEIASTSLVHNLNRPARELVL
                     NWFSRHLAGGRPPELEPVWTGLEDFWPELRAAQDGLDLRDYGLVSRTRREAAERAGSL
                     SGRPAALRAVPFLKRGTRAGRPAAGTGSSVNRRPAWLAPGYRVPFRLAAGRRPAGPTL
                     LIVDQ"
BASE COUNT         1686 a         3828 c         4099 g         1938 t
ORIGIN      
        1 acaagtacca cgaggtgctg gtcttccagg tgctcgaccc ggatgaactc gaactgccct
       61 tcagcgggcc gcacaccttc ctggatttgg agaccggcga ccggctggag accgacgccc
      121 cggccattcg ggccgagtac cggaaactgg tcggcgactt tctcgaaacc taccggaagg
      181 gttgcgccga acacaatatc agttatgtcc gggcccttac caacgatccc tttgatcatt
      241 tcctgcaccg ctacctggag cggcgcgccc gttactcatg aacttcctga acccgctctg
      301 gctctgggcg ctcaccggcg cggccctgcc gatcctggtg cacctgttcg cccgcaagcg
      361 cggcaaggtc ctcaagttca gcacgctcca gttcctgcgt ctttcccaac tgcggaccgg
      421 ccgccggcgc cgcctggagg agatcctggt gctgctgctg cgggtgctgg ccctggcctg
      481 cctgctggcg gccctggccg gcccggtgag ccggtcccgg ctcttcgggg agcgggagac
      541 cgccgcggtg ctgatcctgg acgattcgct cagcatggcc gccgcggtca acggccggac
      601 ggcctccgtc cgggcccggg agctggcccg gtccgctctg gccagcctgc cggagaagag
      661 cgaggtggcg gtgttgacgt tcgccggccg ccggctgccc ttcacccgcc gccgcgacga
      721 cgtcggccgc tggctggacg ggatcgaacc gggctggacg gccggcaacc ttccggcggc
      781 cctcgaggag gccttcggcc tgctggaccg gaagaccggc aaccggatca tctacctttt
      841 caccgacggc caggcctacc cgtggcggaa tgtcgtccga agcgcgaagg acggccggcg
      901 gccggccctg gtgctggccc tggccgggcc gccggcggcc ggcaatctta cctgccgcga
      961 ggtcgaaccg gttccgggcc gccgccgggc ccggtgcacc gtctccaact ggggtgaccg
     1021 gccggccgtg gccgaggtgt cactggccgg cccggcctgg cggctggccc ggagcctgga
     1081 agtggccgcc ggcgagagcc gttcggtcga tttcgacctg ccggaggagg cgggccggct
     1141 ggagggcgga gtggccgcgg ccggcgatcc gctggcggcc gacaacgtct gccgggtgga
     1201 cctgaacgct tccgggccgc gccggatcat gatcgccggg tcgtccggtc cggccctctt
     1261 ctacctggag aaggccctgg cgctcgacgc cgaaacgacc gggctggaac tcttcagccg
     1321 ggagagcgcc ggtctttcag ccacggcgct ggccggctgc ggaatggtga tcctggccga
     1381 tcccggccgc ctggcgccgg ccgtctcggc tttcctgcag gattatgtgc ggtcgggcgg
     1441 caacctgctt tacttcgccg gcgaccgggt cctggccgcc gatttcaacc gcgactggca
     1501 ggcgcccggc ggcaccgagt tcctgatgcc ggcccggatc atcgattggc cgcgcctggc
     1561 ggtgccggtg cggatcgcct gggcggccgc cgagcatccg cttttccttc cctttggaag
     1621 ccgggcgctg gagtacctga aatcggtcaa tttcaaccgg ctggtccggc tggagccggt
     1681 cgacgggacg gtcctgatgc ggggcgagtc gggcctgccg ctgctgctgg agaaacgggt
     1741 gggccggggt cggatcatgc tggccgcctt cctgcccggc gaggattgga ccaacctggt
     1801 cttcaagccg ggtttcccgg tcctgatgca gcacctggcc cgctatctct ccggcggcaa
     1861 tcttctggcc gtcagaacgg gagaggtttt ccgtccgctg gccggcggcg gccgtctttt
     1921 ttctcccgaa ggatcagagg tcccggccga gccggcggcc ggcggctggc gcctggaccg
     1981 gcccggcttc tggcggctgg tgccgaccgc ttccgggacg gcccgggaac gggtgatttc
     2041 cgtaaatccc gacccggagg agggaaatct ggcccgcatc ggcgccgccg aggtccggca
     2101 gcgcctggcg ggcgtcacgg ttgagttcct ggaagcggaa caggcgctgc catttctgga
     2161 acgcggggcg gccgcccggg atctctccga caccctgttg cgcctggccc tgctgctgat
     2221 cctggccgag gcgggcctgg cggccttctt ctaccatttg aaacaccgcg aggggcggca
     2281 tgcttaaact cggctggcag agcctggtgc ccggctggtg gctgatcctg atgctggccg
     2341 gggtctcggc gctgattttt tacgcctatc ggccggtcca tttttccggg cggctgcggc
     2401 ggtggctgcc ggttttgcac gcgctggcgg tagtactgct ggtgctgctc ctggtccagc
     2461 cggaactcgc cctgggcctg cgccgccgga cgaagaaccg gctccatatc ctggttgata
     2521 cctcgctgag catgggaatc aaggatcggc gcggcgtcag ccgcctggac cgggcccgcg
     2581 agttcctcaa ggcgcgccat tatttccggt cgatgcggcc ggtcttccac accgccggcc
     2641 aggagttgac cgaggtcagc cgggaggcgc tggccggcct gaagccggag gcgggcgcca
     2701 gccggatcgc ggccgcggtc cgggacctgg ccgagcgggt cggccgggat ggcgcggcga
     2761 tcctggtcct gaccgacggc gccgatccga cgccgccgga ttgggagggg ttgaagaaga
     2821 accttcggac cccggtttac gcggtcggca tcggcgagag cgagttcaag gacctgagcc
     2881 tggccgagtt ggtggtcaat tcacccgtct accagggaca gacggtgcat atcggggctt
     2941 acgtgcgcca gtccggatac ccgccgggac tggcccggat ccgcctcaag caggcggacc
     3001 gggtcctcca ggaatcgacc ctgcgcgatt cgggtctttt cagttacgcc gaattccagg
     3061 tgccggccgc cgcggccggg gtcttcgagt accaggtcga ggtggcgcgc cagccgaacg
     3121 aggcgctgac cgacaacaac accggcctgg cggtcgtcca ggtggtggcg ccccggatca
     3181 agatccttta ccttgacggc ggcctgcgct gggagtacaa gtttctcaag cgttacctgg
     3241 aatcggatgc ccagctcgaa ccctactttc tggtccgggt gggcggcagc ctcttccagc
     3301 agaccggggg cggcaagctc tccctgccgg ccgatttcct cagccggcgc gattttctca
     3361 agggtttcaa cctggtcatc ctgggcgacc tggacttcac cgcctttacc ggccggcagc
     3421 tggccgccct gcgcgacttc gtgcttgagg acggcggcgg gttggcgctc ctgggcggcg
     3481 acaattttct gcgcggcctg gccggaacgc cgctggaagc gttgctgccg gtggccctcg
     3541 ccggcggcga gtcccgggtg ctgaccgagg ccttcagccc ggcggccgcc gcggtctccc
     3601 agccggttac cttcctggaa ggcctggggc ggctgcccag gctggagcgg cgcaacccgt
     3661 tccgggtacc ggcccgcggc gggctggtcc tgctgacccg ggacggggac cccggccagc
     3721 cggtggcggc ggtgaatctc gctccgcagg ggcggtgcct ggtggtggga accgacgata
     3781 cctggcggtg gtacttcagc ggcgcctcgg aacgacagcg ttacgaggaa ttctgggggc
     3841 gtctcatccg ctacctttgc ggtccggagg agtacctggg catcggccgg cgtctcccgg
     3901 atctgctcct ggaccggcgc gagtacggac tgggcgaacc ggttgcggtc cggctcgcct
     3961 ggcggccggg cggtccggag gggagtccgg aggtcttctg gcaggaccag tccggccgcc
     4021 gggaacgcct cgttttcaaa tcggaggcgg cctccttcac gccgccggcc gaagggcttt
     4081 acctggtggg cgcaacggcc gccggtcgca gcgtcttcaa gtgggtcacg gtcagccgtt
     4141 ccggttccga atggtccgag ccggggcgca acgacgcctg gctggaacgc ctggccgaaa
     4201 ccggcggcgg ccgttacctg cgcctggaat cggccggcgg tttcggtccg gaactggccc
     4261 ggggcgagcc ggtcacctat aacgtttcac tggcgcggcc ggcgcgccgt ttcctgctgc
     4321 cggccgtctt tctgatgctc aacctctgct ggttcctgcg gcggcgccag ggagtgatct
     4381 gaaaccgggg aaatccatca tggaagagcc gagagtcctg atccaggcct acctgcagca
     4441 ctaccggcgg ctggtccagc gccgacggtc ggccgagacc ggctggctgg ccggactctg
     4501 gctggccggc gcgctgctgt ttattctcgc cttcgaatac tttttcggtt tggtcactcg
     4561 ggccgcctgg ctgtcgcgcg ggctgctggc cgcgccgctg gccgttctcc tggtccaggc
     4621 tttcctggag cggcgccgcc tggagcgcct ggaggcggac cggctcgccc tggaactgga
     4681 acggaagttc ccccgcttcg ggaatcacct gatcaacgct taccagctgg ggacccggcc
     4741 gaagggatac ccggcgccgt ttgtcaaccg cctcctgggc gaggcctccg ggatgatctc
     4801 catccgggag ctggatgacc tgccggttcg gccccgggta agctggtacc gcaaggggct
     4861 gctggccgcg gccgccggga tcctgttgct ggcggcggcg gcgacggggc cgatccgttc
     4921 cgggttcctg cgcctcctgg tcccctgggc cggtcccgga acctggggga tcagggtgga
     4981 gccgggcgat tgcctggtgg tgcgcggctc cgacctcgag atccgggcgc tggcggccgg
     5041 ggccggggcg ccccgggtgg agttccacga cggccccggc ggcgcggcag ccatggaacc
     5101 gggaccggcc gggtggtacg cgcaccgttt tctggcggtc cgggagccct ttacctaccg
     5161 ggtccgggcc ggccggagcc tgacgccctg gttccgggtc cgggtgcagg atcccacttt
     5221 tctgaaacgg ctgcggatta attgccggta cccggcctac accggcctgg cggccgccgg
     5281 gtttgaacag gagcccggcg atttaacctt tccggccggg acccggctta ccctggaggc
     5341 ggtcttcaac aacccggtcg gcccggcctg gctggtgctc gggacccggc gcctcggccg
     5401 ttccgggggg cggccggcgg cctcccgccg tttcgaactg accgtggagt cggccaccct
     5461 ttatgaattt cacttttacg attacctggc ccgggattac cgggtcagtc cgaggttccg
     5521 cctgcagccg ggtttcgacc aggcgccggt cctggaattc acggtgccgg ccgccgacct
     5581 ttcagcgtcg gccggcgcca gcatccggct cgaggtcaag gcgaccgacg actacgggtt
     5641 gcggaagatc cgcttccgcc tgcgttcgga ggggaacgac cggccggagg ccgatccggt
     5701 cttcttccag gcccagcttt ccggccgccg cgagggaacg gccagcgcct acctgaagct
     5761 gccggccggc ttttcggaac cgatcgccta ttacgccgag gcgctggaca accacccggc
     5821 cgcgccgaac ctcggccgtt ccagcctcta ccatatctac ccggccgccg gccggtccga
     5881 cccgtccggc cgccgcggtc gggaggagga cgggaaccgg ccgccggaaa tcgaaatcct
     5941 caaggaactg gccgaaaacc tgaagcagtt cgccgaggag cagaagaaac tgctggaggc
     6001 ggccaagcgg ctggataagc cccaggacct gcgggccgcc gagaaggcgg ccgacctggc
     6061 cgaggtcgaa cgcaagtggg ccgaactctt ccagaagatg gtggatgacc tgaacaagat
     6121 caaccagcag accaagggaa agttcacgct cgcccaggaa ctggtggaga tggtctccca
     6181 cctgcagaag gcggccgagg cgcttcctca caagccggtc cacatggtca ttccggaatc
     6241 gatggccggc ctggaactgg ccgaggaact gacggccaat ctcgaacgct ggatggccga
     6301 ggagcccgat tacctgaagt gggatatggc ggagccctcc aagcgggtcg aggcgcctga
     6361 ggcggaactg cccgacgagc tgatggacat cgtcggcgat ctcctggagc aggaggagga
     6421 catgaaggag gagatcgagg acatcaccag cagctggatg gacagtatgg acaagggggc
     6481 cggctggacg gccatggacg gaccgatcag caacatgagc gccaagggca tcaccgggaa
     6541 cctcatgccc aaccagcacg aggtgggcgg ccgcgccggc gaggggcgca ccggccgttc
     6601 ctacggcgag atggtcgagg agaccgcggt cggcaagggc ggccggcgca ccccggcccg
     6661 gctgaccccg gacaaccggg aaccgggttc aatcaaggat tccagcggcg aaaacccgct
     6721 cggtccgacc ggcggcggca agagcagcgg ctggggtgcc gaggggctcc aggggccggt
     6781 ccaggatctg aacttccgtt acgaccagct ggccggccgc caggcccggt tggtggagga
     6841 ggccgagaag ctggaacgga aattgcaggt gcttaacgtt tccaacccgc agctggaaca
     6901 ggccttatcc tcgatgcgca atttccaggt ccagttgagc agcggccgct acgaggatct
     6961 gcttacgacc aagcagttga tcatttccaa cctccagcag gtccagcagt caatagtcgg
     7021 ccagacggtc gtccgcctgg aggggagcga acgctcggtc cggcggaacc gggaattgcg
     7081 cagcgcctgg gaggaccggg ttccggccgg ttacgagggg ctggtccagc gttactaccg
     7141 ggacatctcc ggacattgag ggaggctggc ttgcgaaacc tgacctggct gccggcgctg
     7201 tttcttttgg ccggcctggc ggcgtcggcc gaggccgccg gcggactgaa cctgtttctg
     7261 aattcgcccc gggccgttta cgccgaggag gaggaggtgc ggctggatct gatgatcgag
     7321 gccgaggcgg accggtcggt ctccttccag tatctccagc ccaaggagga cggttttctc
     7381 ttcgttggcg gcgagtggcg gctggaggtc cgggtcaacg gggtgaccgt cccggagttc
     7441 accaaccaca tgccgccgga gccaaaggcc gggacgctcc agctgaacct gtcgcccggc
     7501 gcctccttca gtgcccggat tcccttttac tattactact acccgtttga gctgccggcc
     7561 gatttcgagg tccgcctcca ttacgacggg agcgtctcca acccggtccg ttttcaggtc
     7621 cggcgcaccg aaggacgtcc ggtcgccggc ggctggctga tcaacggcga tttccgttcc
     7681 ggccgggact ttccgctggg ctggcggctg accggggacc gggtgcgctg ggagaaggag
     7741 ccgccggccc tccggctgac cctggaccgg gccacggccg aggggccggg gctctgggtc
     7801 tactcgacct tccaggagct gaaggcgccg gccgagttca cccttaaggt ccgggcccgc
     7861 agccaggggc aggagatcat tgtttttgtc gagggctggg gtctggtggc cggccggcgc
     7921 cggctgctgg aacggaacga gtgcttcatg catccggccg ggacggaatc ggccgattac
     7981 cggacggcgg ccgtcttcag aaacccgcag gtacgctggc tgcgggtcaa ggtttacacc
     8041 tacctcaagg tgggcgacat ccggtttgaa tcggtggacc tggtgccgaa ggagtgagcg
     8101 gtggagcgat taaaaaattc aataatccgg ccggcggcgc cgctcttatg ggcggccacg
     8161 gttttcctga tgctggccgc ggccgcgggc accgcttcca gccaggcgga ttggccggag
     8221 ggctgggagt accggatggc cctggaggtg gattccggcc gtctggacgc cgccggggat
     8281 cggctggcca tccttgattt ctgcggggcg gcccggcccg acggggccga cctgcgcctc
     8341 tttgacgagt cggggagttc ggtgccgttc caattgctgc aggccgggcc ggggcagcgc
     8401 taccggatcc tctttccggc ctcccgcggc cgttgccggc tctatttcgg gaacccggcc
     8461 gcggccgccc cggcaccggc cgattttccg atccggcgcg gtctgtggct ggaggtttac
     8521 gagcgcaagg gagaccgggt ggacacctgg gaggaggccc gggcggtggc ggaggccagc
     8581 cgccggaccg gccgccgcct gggccgggcg gcctggaaac aggtctggga cggggccaat
     8641 cccttcggtc cggagaagga cgtggttaaa atttacaccg gttacttccg atccgaagcg
     8701 ggcgtctacg agttcgccac cagttccggc ggcgcctctt tcctgctgat cgacggccag
     8761 ctgacggccg cctggccggg ctggcacggt gcggaaccgt ttatccggcc ggcccggacc
     8821 gggaaagtga cgctgaccgc cgggattcac gaactggcct attaccagtt cagccgttcc
     8881 ggccaggaga tcacggccgc cgccgtcaag cggccgggcg agaaggattt ccaggtgatt
     8941 ccggccgact ttttccttcc gctggccggg gcgatcccgg tcaacctgga acggcggggc
     9001 aagccggtgg cggccgccct gacctgggag aacacccatt atctgcgccg ggaaaactgg
     9061 gagttgctga cctttcgttt ctttgacctc tcccacccgg acagcgaaat agaggtgcgc
     9121 cagtgggact tcggggacgg ccagtcggcc gtcggtcgca acccgttgca cacctacctg
     9181 aaaccgggcc tctacccggt gaccctgacg gtgcgcggcc gccggggcgc cggatccgac
     9241 cgggtccggt tgacggtgcc ggttgagcag aattacggag agatgcgcct gccatcccgg
     9301 agtcttgagg cctacctgga ggagttcgac cggcttgacc tggataggct cgggaacgag
     9361 gaactgggca tcctggccca gctctaccag agttacgacc gttccgaggc ggcctaccgt
     9421 tgttaccgga atctggcggg ccgccgtctt tcgagtgagg agcggcgtcg cgccggagcg
     9481 gtggcggccg cgctggccgt ccgggaacgg gattacgccg gggcggccga actctaccgg
     9541 cagctgctgg ccgagaggcc ggcgccggaa ttcctggtga aactcgggtg ggtccagctc
     9601 cggctcgggg aactggacca ggccgacacc ctctttcgcc aggccgaaac ggccgccggg
     9661 gcccggccgg aggagcggcg cctggcgacc atcggccggg gggacgtcct ccggcagcgg
     9721 ggtgaggcgg aggcggcccg ccaggcctac ctgcccctga ccgagcgcgg caacctggag
     9781 tcgcgcaacg gctggtacga ccagcaggtt ctttactatg tcaggcagaa ggattttttt
     9841 accgcctggg agcgtctgga ggcctggggg gaggaactgc ccctggttaa gctggaggga
     9901 gcctggtcca tcctggcggc ccggatcggc ctggagcgca aggattacga tggagcccgc
     9961 caggaactgg agaccttcct gaaggttacc gattccgaag ccggcaacgc ctaccggccg
    10021 ggtgccgttt atctgctggg ccaggtctgg gaggcgcagg gagagcgggc ccgggcccgc
    10081 ggtttctacc agcaggtggt tgaccggtat cccgggactt cctgggccgg gctggccgcc
    10141 gaccggatca aaaaataaaa tctgatttta aaggaggcca ccgctgatga agaaaaaagg
    10201 gaccgggacg caagggaaac cgctgcctta cctgaggttg gagtccatcg gccgcgccgg
    10261 ctggctccgg gccgaccgcc gcttggagcg gatcggcggc tggtcggacc tggccgccgt
    10321 ccaggccgaa ctgaaccgga tcttcgaaga aatcttcggc ccccggcccg acccggcggc
    10381 cccggtgcct ttccgggaac tggctggctt ctccttcgcc ggggtcgaga tccggcaggt
    10441 tctcctggaa ccggaacccg gttaccggat ttccggcttc accttccggt ccggctccgg
    10501 ttccggcccc ggtccggccc ttgtggtggc ctccggacac gtcgagcgcg ccgccctcta
    10561 tctcccctac ctgaaattgt gcctgtacgc ggctcagaac ggcttctacg tgctggcctt
    10621 cgatccggtc ggccagggtt cgcgccgcga ggtcttcctg cccggtgccg acccggagta
    10681 ctggtcgcct tgcgaccagc accaggatct ggccgccgtc gccatgctga ccggacgcca
    10741 gctggcccat tacctggtgc gggacaacac gagcgcgatc tccttcgtcg ccagccggcc
    10801 ggaggtcgat ccggagcgga tcggtttcac cggacaatcc ggcggcggaa tccagacctt
    10861 cctggcgctg gccgactccc gggttagggc ggccgttccc atgcaggcca cctccagccg
    10921 gcgcggcgcc ttccagcacc tggtgatgcg ggacatggac cagatcttct ggcattgctg
    10981 ggaacgcggt ttcgaccaga gcgaactggt ggccctcttc gcgccccggc cggtgcggat
    11041 catcgccgaa cacggctacc cggaccaggt tgatatctac cggaggctgg aaccggtcta
    11101 ccgcaaactc ggccttcccg aacaactgga aatcgcctcg accagcctgg tgcataacct
    11161 gaaccggccg gcccgcgaac tggttctgaa ctggttcagc cgccacctgg ccggcggccg
    11221 gccgccggag ctggaaccgg tctggaccgg actggaggac ttttggccgg aactccgggc
    11281 cgcccaggac ggcctggacc tccgggatta cggactggtc agccgcaccc gccgggaggc
    11341 ggccgaacgg gccggttctc tttccggccg gccggcggcg ctccgggccg tgccgttcct
    11401 gaaacgggga acccgggccg gccgtccggc cgccgggacc ggctcttcgg taaatcggag
    11461 gccggcctgg ctggcccccg gctaccgggt gcccttccgg ctggctgccg gacgccggcc
    11521 ggccgggccg accctgctga ttgttgacca g
//
LOCUS       DAOQYN010000033        11540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_6054_length_11540_cov_3.916239, whole genome shotgun sequence.
ACCESSION   DAOQYN010000033 DAOQYN010000000
VERSION     DAOQYN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11540
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6054_length_11540_cov_3.916239"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PKN80_02420"
     CDS             complement(<1..483)
                     /locus_tag="PKN80_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34899.1"
                     /translation="MLRIKKIGMANGRRTQKWLALGLAALTLLFGAACGGSGGGSGIR
                     WPVLPEGDLYYSQEGWARMNEAGTNIVTDDYTLVDSHLDDTQNLVFNGNPMLLEGYQF
                     WYNCFSEEGFTLFLEPDDPQTEISRLSVAIHNKGMNLTPAAVEFIEQDPVNNAFVVLS
                     L"
     gene            661..975
                     /gene="secG"
                     /locus_tag="PKN80_02425"
     CDS             661..975
                     /gene="secG"
                     /locus_tag="PKN80_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNQ34900.1"
                     /translation="MVTLLIAVHILVSLLLVGAVLIQSGKGAASANIFGGARAAENVF
                     GASTPKVINGITAALAAAFIITSTLMTVFVGRAQTSVIKRSLSLPAQPAQPLPAPDQP
                     LE"
     gene            1105..2742
                     /locus_tag="PKN80_02430"
     CDS             1105..2742
                     /locus_tag="PKN80_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ34901.1"
                     /translation="MSLLLAGCQASRRITSRPDTLVLSTTSDPKTFNSILAQESSSTA
                     VTAYLFEGLTRMNGVTAEVEPGLAESWQVSPDGRVWEFKLRPARWMDGRPVSADDVVF
                     TFRDLIYNPEVPTSSRDVFLIAGRPIKVEKVAEDRVRFILPAPFAPFLRQLGQEILPR
                     HRLLPALQAGRFNTAWGVDTPPEALIGNGLFSLREYRIGERLVFQANPGYWMKDAQGR
                     ALPYLKRVVMLVVPDQNAELLKFLQGEVDAISVRGQDYRLLKPQERRRNFTLYNAGPT
                     LGNSFLVFNQNPDAPIRPEKLRWFQDRRFRQAVSYALDRESITRNVFAGLAYPQWGPL
                     NPGNGYFYNERVRKYPYDPELSRRLLAEAGFRWKDGTLQDRDGRPVEFTLLTNGDNQQ
                     RVQTSTIIMDDLARLGIKVHFLPVDFNNLVTRLNTSFEWETVLIGFTGTIDPHGGRNV
                     WHSSGQLHVWNPRQPKPRTVWEREIDDLFERGAVEMDPGRRKAIYDRWQEVAAEEQPL
                     IFTVNPAALYAVRNRFGNLKPTIYGVFHNLEAIYTLD"
     gene            2749..3234
                     /locus_tag="PKN80_02435"
     CDS             2749..3234
                     /locus_tag="PKN80_02435"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNQ34902.1"
                     /translation="MKLMVRYRKTGPVRFISHLDTERFWRRAVRRAGLPAVRTRGFSV
                     REKIELGYPLPVGCESEGEDFVLELEGEIEPETVADRLAAVMPEGFGVAGVFPYREKE
                     SLFIRTRTLVYQVEGKRVELPVENGRSPRLWPFLSEYFGCSEAEAKLFRVTRVEIRYR
                     E"
     gene            3274..4791
                     /locus_tag="PKN80_02440"
     CDS             3274..4791
                     /locus_tag="PKN80_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNQ34903.1"
                     /translation="MSRIVMDSAGFETRAAVLSNGRLEYIEIERLNRIGLVGNVYKAQ
                     VVRVLQGLNAAFLNIGESREAFLPLTDLHEDSLYGEEEDDEAAPEKKEPERDKRRAPS
                     RRLRLRPRDQVLIQVIKDSIGTKAPKVTANHISLPGYYLVLLPQKPRRGISRRINNQA
                     ERDRLLAAVSEVIPAECGFIIRTSAEGCGERHIKAEADSLWKTWQEITGRGQSMSAPV
                     RLYSELELPLRMLRDRYTKEIEQVMVDSPLLLKSCRQYLGRLGMDGRRVSLHQGRKPL
                     FIEHKVDEEIHKMVARKVPLEGGGYLVIDETEALCAIDVNSGRTGGDDYRSMVLRTNL
                     KAAEEIAWQLRLRNVGGLIVVDFIDMDRHQDRRRVYQSFEKALEPDKARIKVLQISRL
                     GLVEMTRQRTRTTLRDYLAEDCPVCGGQGQVSSAETVAAQLRRDLAPELKNQPGIPLL
                     RRFTRRRIEVKISRSLDDYFKSNLQLVFPERNYRQMLNLVVDPALPFNGYRIEGR"
     gene            complement(4795..6201)
                     /gene="gltA"
                     /locus_tag="PKN80_02445"
     CDS             complement(4795..6201)
                     /gene="gltA"
                     /locus_tag="PKN80_02445"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNQ34904.1"
                     /translation="MAIEKKAVPMREQPASERIGNFNEVPLGYDEAEAIHEARRCLQC
                     RKKPCVDGCPVRIEIPAFIKALREGDFRKALEILHDTNSLPAVTGRVCPQEDQCQLKC
                     VLGRNGEPISIGRLERFLADWEYRQRGEPKAEPPRLSGARVAIIGSGPAGLTCAAELA
                     KTGYQVTVFEGFHQLGGVLVYGIPEFRLPKDIVAAEIEYLKGLGVHFATDVLIGRALT
                     IQDLFHDGFQAIFLGTGAGLPVFMHIPGENLPGVYSANEFLTRVNLMKAYRFPEFDTP
                     IKKGRRVAVIGGGNVAMDAARTALRLGAEKVFLIYRRSEPEMPARREEVIRAREEGIE
                     FLLLAAPVRYWPDQNGFVHEAECIRMELGEPDASGRRRPVPMEGSNFRIQVDEVIVAV
                     GTTPNPIIARTTPGLKTQRRGEIEADESGRTSLPGVFAGGDAVTGAATVITAMGAGRQ
                     AARAIDEYLRGRTAESKS"
     gene            complement(6191..7027)
                     /locus_tag="PKN80_02450"
     CDS             complement(6191..7027)
                     /locus_tag="PKN80_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014835388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNQ34905.1"
                     /translation="MFEILEKYYLGPKIQYALVKAPLIARKAEAGQFVILRVNEKGKR
                     FPLTIADFDRDGGTITLVFQEMGKSTHLLGQLSAGDHIMNVLGPQGNPTEVEKFGRVV
                     VVGGGIGVAPVFPLARKLKEHGNEVTAIIGFRSKEHVFWEERMRSAADRLLIATNDGS
                     YGEKGFVTEVLARRLESGPADRIFASGPAVMMKAVADLTRPLGIKTIVSLNSMMVCGM
                     GMCGACRATVDGKTRFTCLDGPDFDGHQVDFDELISRLGTYREEENVSFERWRKEAKH
                     GN"
     gene            7322..8116
                     /gene="iolB"
                     /locus_tag="PKN80_02455"
     CDS             7322..8116
                     /gene="iolB"
                     /locus_tag="PKN80_02455"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HNQ34906.1"
                     /translation="MEYQKHCQPRPGLNRVLPPGAMGLKYLELDLLDLGPGETHRETL
                     DGKEAVLTILAGSGLVETGQFSRELGGRADVFSGRPEGAYLGEGAAWSIRAVTRLEAA
                     IFRSPAAERLPAYPVAAAEVQPERRGQDLFVRQVSDLVGLSRPAGRLIVGETLSEPGH
                     WSSYPPHKHDRDNLPEESFLEEVYYFRIAPADGFGFMRLYTEDGRLDQPILLKDRDLV
                     SIPRGFHPVAVAPGYRVYYLWALAGPSRNMKLREDPSHARLGVRKA"
     gene            8172..9596
                     /gene="gnd"
                     /locus_tag="PKN80_02460"
     CDS             8172..9596
                     /gene="gnd"
                     /locus_tag="PKN80_02460"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNQ34907.1"
                     /translation="MEKKADIGLIGLAVMGENLVLNMESRGFTVAVFNRTVEKVDAFV
                     AGRAKGRKIIGCHSVEELASQLKSPRKVMLMVKAGEAVDNFIDLVKSHLDAGDIIIDG
                     GNSLFTDTIRRTRKLESEGFRYIGTGVSGGEEGALHGPAIMPGGSASAWPEVQAIFEK
                     IAAKAPADGKPCCTWIGPDGAGHHVKMVHNGIEYGDMELIVEAYYIMRYVLGIPAAEQ
                     SEIFKKWNQDVLSSYLNEIAGDILGRVDPDTGRPLVEVILDKAGQKGTGKWTSQNALD
                     LGVPAPTIAEAVFARCMSALKDERVKAAAILPGPKAKFTGDKAAYVEAIHAALYCSKI
                     CSYAQGFGLMREAAREYKWNLDFGKISLIWRAGCIIRAQFLQKIADAYGRNPELANLL
                     LDEYFQKSIAERQEAWRQVVSDAARLGIPAPAFASALAYYDGYRSEVLPANLIQAQRD
                     YFGAHTYERVDRPGTYHTNWVEEK"
     gene            9596..10405
                     /locus_tag="PKN80_02465"
     CDS             9596..10405
                     /locus_tag="PKN80_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ34908.1"
                     /translation="MFDVKGKVAVVTGAGGVLCGGMAALLAEHGVRVAVLDIFEDKAA
                     AVAAAIRSAGGQAISCKADVLSKASLEEACRTVLAEFGTVDILVNGAGGNKKEATTSP
                     DLSFFDLPADAFQWVFNLNFIGSVLPSQVFGRVMAEKGGGSILNISSMNSFRPLTRIV
                     AYSAAKAAINNFTQWLAVYLAQNCSPRIRVNAIAPGFFLTEQNRFLLTDEKTGELTPR
                     GQTIIGHTPMGRFGEPADLNGTILWLLSDASSFVTGIVVPVDGGFSAFSGV"
     gene            10432..11295
                     /locus_tag="PKN80_02470"
     CDS             10432..11295
                     /locus_tag="PKN80_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNQ34909.1"
                     /translation="MDLKNFPKRFDWFVGIDSDGCVFPSMDIKQKECFHPAIMDQWGL
                     WKVEKEVRMVAEWVNLYSVHRGVNRFPGLRMTFDFLREMDSVRAAGIAIRETGDLEAY
                     IKSGLPLGNDTLKTYVQEHPSLADVLEWSLEVNRRVAATVKEVPPFPFVRESLEKLRP
                     RVDMIVVSQTPTEALVREWKEHAIDGLVDFIAGQEQGSKKEHLQMAAAPNYPADRILM
                     IGDALGDLKAVEAVGGFFFPINPGHEAESWENFYQEGIERFLAGSFKGAYQEKLMAAF
                     KALLPEQPHWK"
     gene            11303..>11540
                     /locus_tag="PKN80_02475"
     CDS             11303..>11540
                     /locus_tag="PKN80_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34910.1"
                     /translation="MVRLSSRERLTRTLAGEPVDRVGISLYEFDGHYESWIHRFPEYQ
                     ALLDYAAGRTDRLFFWAPASPSPALFYGQVEPDTV"
BASE COUNT         2046 a         3653 c         3800 g         2041 t
ORIGIN      
        1 gagcgacagt acgacaaaag cattgttgac cgggtcctgc tcgatgaatt cgacggcggc
       61 cggggtgagg ttcatgccct tgttatggat ggccacgctg aggcgggaga tctccgtctg
      121 cgggtcatcc ggctccagga agagtgtgaa gccctcctcg gagaagcagt tgtaccagaa
      181 ctggtacccc tccagcaaca tcgggtttcc attgaagacc aggttttggg tgtcgtccag
      241 gtggctgtca accagggtgt aatcgtcggt tactatattg gtaccggcct cgttcatcct
      301 tgcccagcct tcctgggagt agtaaaggtc gccttccggg agcaccggcc agcggatgcc
      361 gctgccgccg ccgctgccgc cgcaggcggc tccgaagagc agggtgagag cggccaggcc
      421 gagggccagc catttttggg tgcgccgtcc attggccatc ccgattttct tgatccgcag
      481 catgccgcct ccgctggttg gaaaattagt taagttgacc ggaaggatcc cgccaaattg
      541 aatattaagg gaaataataa ctattttcgc cgtttttgtc aaagagggag ggggcggccg
      601 gccggtcgtg atcgggacgg cggttgacga ggatgtggca gaaggcatat aataaaaaga
      661 atggttactt tattgatcgc agtgcatatc ctggtaagcc tgctgctggt cggcgccgtg
      721 ttgatccaga gcggaaaagg cgcggcttcg gccaacatct tcggcggcgc ccgggcggcc
      781 gagaacgtct tcggggcctc gacgccgaag gttatcaacg ggattaccgc ggcgctggcg
      841 gcggcgttca tcataacctc caccctgatg accgtcttcg tcgggcgcgc tcagacatcg
      901 gtcatcaagc gcagcctgag cttgccggcc cagcccgcgc agccgcttcc ggccccagac
      961 cagcccctgg aataagttca ccgcgtgtcc cacctccagg cgttcaactt ccggcgcggc
     1021 ttcgggccgc gcgccggatc cccggcacca cccagacggc gccggggatc tccctggttc
     1081 cgggtctgtc tcggcggcct gggcttgtct ttgctgctgg ccggctgcca ggcctcgcgc
     1141 cggatcacct cgcggccgga taccctggtc ctgtccacca cctccgatcc caagaccttc
     1201 aattccatcc tggcccagga gagcagttcc accgccgtaa ccgcctacct ctttgaaggc
     1261 ctgacccgga tgaacggggt caccgccgag gtggaaccgg gcctggccga aagctggcag
     1321 gtcagccccg acggccgggt ctgggagttc aaactgcggc cggcccgctg gatggacggc
     1381 cggccggtca gcgccgacga cgtggtcttc accttccggg acctgattta caacccggag
     1441 gtgccgacca gttcgaggga tgtcttcctg atcgccgggc ggccgatcaa ggtggaaaaa
     1501 gtggccgagg accgggtcag gttcatcctg ccggcgccgt tcgcgccctt cctgcgccag
     1561 ctgggccagg aaatcctgcc ccgccaccgg ctgctgccgg cgctccaggc cggccgtttc
     1621 aacaccgcct ggggagtgga tacgcctccg gaggcgttga tcgggaacgg acttttcagc
     1681 cttcgggagt accggatcgg cgaacggctg gtcttccagg ccaatcccgg ttactggatg
     1741 aaggacgcgc agggaagggc gctgccgtac ctgaagcggg tggtgatgct ggtggtgccg
     1801 gaccagaacg ccgagttgct gaagttcctg cagggcgagg tggacgccat ctcggtgcgc
     1861 ggccaggatt accggcttct gaagccgcag gagcggcgcc ggaacttcac cctctacaac
     1921 gccggcccca ccctggggaa cagcttcctg gttttcaacc agaacccgga cgctccgatc
     1981 cgtccagaga aactgcgctg gttccaggac aggcggttcc ggcaggcggt ctcttacgcc
     2041 ctggatcggg aaagcatcac ccggaacgtt tttgccggcc tggcctaccc ccagtgggga
     2101 ccgctcaatc caggcaacgg ttacttctac aacgaaagag ttcggaagta cccgtacgat
     2161 ccggaactga gccgccgcct cctggccgag gccgggttcc gatggaagga cgggacactg
     2221 caggaccggg acggccggcc ggtggagttc accctgctga ccaacggcga caaccagcaa
     2281 agggtgcaga cttcgaccat catcatggac gacctggccc ggctcgggat caaggttcac
     2341 ttcctgccgg tggatttcaa caatttggtg acgcggctga acacctcctt tgaatgggag
     2401 acggttttga tcgggtttac cgggacgatc gatccgcacg gcggccggaa cgtctggcat
     2461 tccagcggcc agctccatgt ctggaacccg cgccagccga aaccccggac cgtttgggag
     2521 cgggaaatcg acgatctgtt tgaacggggg gcggtggaga tggacccggg gcggcgcaag
     2581 gccatctatg accgctggca ggaggtcgcc gccgaggagc agccgctgat cttcaccgtc
     2641 aacccggcgg ccctctacgc ggtccgcaac cggttcggga acctgaagcc gaccatctac
     2701 ggggtctttc ataacctgga agcgatttac accctcgact gaccgcttat gaaactgatg
     2761 gttcgctacc ggaagaccgg cccggtccgc tttatctcgc acctggatac cgagcgtttc
     2821 tggcgccggg cggtccgacg cgccggcctg ccggcggtcc ggaccagggg tttttcggtg
     2881 cgcgagaaga ttgagctcgg ttacccgctg ccggtcggct gtgagagcga aggagaggat
     2941 tttgtcctgg agctggaggg cgagatcgaa ccggagacgg tggccgaccg cctggccgcg
     3001 gtgatgccgg aggggttcgg ggtggcgggg gtctttccgt accgggagaa ggaatcgctt
     3061 ttcatccgga cccggacgct ggtctatcag gtcgagggaa aacgggtgga attgccggtg
     3121 gagaacgggc gcagccctcg attgtggccg tttttgagcg agtatttcgg atgttccgag
     3181 gcggaagcca aactcttccg ggtcacccgg gtcgagatcc ggtaccggga gtgaaaactg
     3241 aattttttga acaacaacga aagtgaaggg gatatgagtc ggatagtgat ggacagcgcg
     3301 ggttttgaga cccgggccgc cgttctttcg aacggccggc tggagtatat cgagattgaa
     3361 cgcttgaacc ggatcggcct ggtcggaaac gtttacaagg cccaggtggt cagggtgctg
     3421 cagggattga acgccgcctt tctgaacatc ggcgagagcc gggaggcgtt tttgcccctg
     3481 acggacctgc acgaggattc cctctacggg gaggaagagg acgacgaggc ggccccggag
     3541 aagaaggagc cggagcggga taagcggcgg gcgccgtccc gccgtttgcg gctgaggccg
     3601 cgcgatcagg tcctgatcca ggtcatcaag gattcgatcg ggaccaaggc ccccaaggtg
     3661 accgccaacc atatctccct gccgggctac tacctggtgc tgcttcccca gaagccgcgt
     3721 cggggcatct cgcgtcggat caacaaccag gccgagcggg atcgtctgct ggccgccgtg
     3781 agcgaggtca tcccggccga gtgcggcttc atcatccgga cctcggccga gggctgcggg
     3841 gaacggcaca tcaaggccga ggctgactcg ctctggaaga cctggcagga gataaccggg
     3901 cgcggccaga gcatgagcgc gccggtacgc ctttattccg agctggaact gccgctgcgg
     3961 atgctgcgcg accggtacac caaggagatc gagcaggtaa tggttgattc gcccctcctg
     4021 ctcaagtcct gccgccagta cctggggcgg ctgggcatgg acggccgccg cgtgagcctg
     4081 caccaggggc gcaagccgct tttcatcgag cacaaggtgg atgaggagat tcacaagatg
     4141 gtcgcccgca aggtccccct ggaggggggc gggtacctgg tgatcgacga gaccgaagcc
     4201 ctgtgcgcca tcgacgtcaa cagcggccgg accggcgggg acgattaccg gagcatggta
     4261 ctgcggacca acctgaaggc ggccgaggag atcgcctggc agctgcgcct gcgcaacgtg
     4321 ggcgggttga tcgtggtcga tttcatcgac atggaccgcc accaggaccg gcgccgggtc
     4381 tatcaatcct ttgagaaggc cctggagccc gacaaggcgc gcatcaaggt gctccagatt
     4441 tcgaggctgg gactggtgga gatgacccgc cagcggacgc ggaccaccct gagggattac
     4501 ctggcggagg attgcccggt ctgcggcgga cagggacagg tatcttcggc cgagaccgtg
     4561 gcggcccagt tgaggcggga cctggccccc gaactgaaga accagcccgg catacccttg
     4621 ctgcgccgct tcacccggcg ccgaatcgaa gtcaagatct cccggtcgct ggatgattac
     4681 ttcaagtcca acctgcagct ggtctttccg gaacgcaatt accggcagat gctgaacctg
     4741 gtggtcgatc ccgccctgcc gttcaacggt taccggatcg aggggcggta gagctcagga
     4801 ttttgattcg gcggtccggc cgcgcaggta ttcgtcgatc gcccgggcgg cctggcgtcc
     4861 ggcccccatg gcggtgatca ccgtggccgc tccggttacc gcgtcgccgc cggcgaagac
     4921 tccgggcagg gaggtgcggc cggattcgtc ggcctcgatt tcgccccggc gctgggtttt
     4981 gaggccgggc gtggtccggg cgatgatcgg gttgggtgtg gttcccaccg ccacgatcac
     5041 ctcgtcgacc tggatccgga agttggaacc ctccatcggc accggccgcc ggcggccgga
     5101 agcgtccggt tcgcccagct ccatccggat gcactcggcc tcgtgcacga agccgttctg
     5161 gtcgggccag tagcgaaccg gcgccgccag cagcaggaat tcgattccct cctcccgcgc
     5221 cctgatcacc tcttcgcgcc gggccggcat ctccggctcg gagcggcggt agatcagaaa
     5281 taccttctcg gcgcccagcc gcagggcggt ccgggccgcg tccatggcca cgttgccgcc
     5341 gccgatcacc gccacccgcc gtcccttctt gatcggggtg tcgaactccg ggaagcggta
     5401 ggccttcatc aggttgaccc gggtcaggaa ctcgttggcc gagtaaaccc cgggcaggtt
     5461 ctcgcccggg atgtgcatga agaccggcag gccggctccg gtgcccagga agatggcctg
     5521 gaatccatcg tggaagagat cctggatggt gagggcccgg ccgatgagga cgtcggtggc
     5581 gaagtggacg cccagtccct tgaggtactc gatttcggcg gccacgatat ccttgggcag
     5641 ccggaactcg ggtattccgt aaaccagcac accgcccagc tggtggaaac cctcgaagac
     5701 ggtgacctgg tacccggttt tggccagttc ggccgcgcag gtaagtccgg ccggcccgga
     5761 accgatgatg gcaacccggg ccccggagag ccggggcggt tcggctttag gctcgccccg
     5821 ctgccggtac tcccagtcgg ccaggaatct ttccagccgg ccgatggaga tgggttcgcc
     5881 gttgcggccc agcacgcact tcagctggca ctggtcctcc tggggacaga cccgcccggt
     5941 caccgccggc agcgagttgg tgtcgtgcag tatttcgagc gccttccgga agtcgccctc
     6001 ccggagcgcc ttgatgaagg ccgggatttc gatccggacc gggcagccgt ccacgcaggg
     6061 cttcttccgg cactgcaggc agcgccgggc ctcgtgaatc gcttcggcct cgtcatatcc
     6121 cagcggcacc tcgttgaaat ttccgatgcg ctcggaggcc ggctgctccc gcatcgggac
     6181 cgctttcttt tcaattgcca tgcttggcct ctttgcgcca gcgttcgaag gagacgtttt
     6241 cttcctcgcg gtaggttccc agccggctga tcagttcatc gaaatcgacc tggtgaccgt
     6301 caaagtcggg accgtccagg caggtgaaac gggtcttgcc gtccacggtg gcccggcagg
     6361 ccccgcacat gcccatgccg cagaccatca tggagttgag gctgacgatg gtcttgatgc
     6421 cgagcggccg ggtcaggtcg gccaccgcct tcatcatcac cgccgggccg ctggcgaaga
     6481 tccggtcggc cggacccgat tccagccggc gggccaggac ctcggtgaca aagcccttct
     6541 ccccgtaaga gccgtcgttg gtggcgatca gcagccggtc ggcggctgac cgcatccgct
     6601 cctcccagaa gacgtgttcc ttggaacgga aaccgatgat ggcggtcacc tcgttgccgt
     6661 gctccttgag cttgcgggcg agcgggaaga ccggggcgac cccgatgccg ccgccgacca
     6721 ccaccacccg gccgaatttt tccacttcgg ttggatttcc ctggggtccc agaacgttca
     6781 taatgtggtc gccggccgag agctggccga gcaggtgggt gcttttgccc atctcctgga
     6841 agaccagggt gatggtcccg ccgtcccggt cgaaatcggc gatggtgagc gggaaccgct
     6901 tgcccttttc gttgacccgc aggatcacga actgtccggc ctcggccttg cgggcgatca
     6961 ggggggcttt caccagggcg tactgtatct tgggacccag gtaatacttt tccaggattt
     7021 cgaacatccg gttctccggc ttggttggtt gaaaggggat gcctgtggaa cagaaacgga
     7081 aaaacgagtg tttaatatat catagaaccc aaaaaaaatc aacgttgccg cgcttccggg
     7141 ccgggttggc gggttgaccg gaggccccgg ctgatgttat cattaatagg tttcaaaacg
     7201 gtccgtcccg gccgcgtttc cgcccgatca acggcggccg gccggtggtt tccgcaatcc
     7261 ctgcagcaac tttccgcgat ccgtttccgc caagtcgaag atttgtaacc gggagacgac
     7321 catggaatac cagaagcatt gtcaaccccg gccgggtctg aaccgagtcc tgccacccgg
     7381 cgccatgggg ttgaagtacc tagagctgga cctgcttgac ctggggccgg gagagacgca
     7441 ccgggagacc cttgacggca aggaggcggt gttgaccatc ctggccgggt ccggcctggt
     7501 cgagaccggc cagttcagcc gcgagctcgg cggtcgggcc gacgttttct ccggccggcc
     7561 ggaaggggcc tacctggggg agggcgctgc ctggtcgatc cgggccgtca cccggctcga
     7621 ggccgccatt ttccgctccc cggccgccga acggctgccc gcctacccgg tggccgccgc
     7681 cgaggtgcag ccggaaaggc gcggccagga tctcttcgtc cgccaggtct ccgacctggt
     7741 cgggctcagc cgtcccgccg gccggctgat cgtgggcgag accctgagcg agccgggcca
     7801 ctggagcagt tatcctcccc acaagcacga ccgggacaac ctgcccgagg agagcttcct
     7861 cgaggaggtt tactacttcc ggatcgcacc ggccgacgga ttcggtttca tgcgtctcta
     7921 caccgaggac ggccggctgg accagccgat cctgctcaag gaccgcgacc tggtttcgat
     7981 cccgcgggga ttccacccgg tggcggtggc gcccgggtac cgggtttatt acctctgggc
     8041 cctggccgga ccgagccgga acatgaaact ccgggaggat cccagccatg cccggctggg
     8101 cgtcaggaag gcctgaaacc ggaaacataa acagacggaa ttcaccttaa tttacaaacg
     8161 gaggaaccgg gatggagaag aaggcggaca tcggattgat cggattggcg gtcatgggcg
     8221 agaacctggt gctgaacatg gaatcgcgcg gtttcaccgt ggccgtcttc aaccgcacgg
     8281 ttgaaaaggt cgacgccttc gtggccggcc gggccaaggg caggaagatc atcggctgcc
     8341 acagcgtcga ggagctggcc agccagctca agagcccgcg caaggtcatg ctgatggtca
     8401 aggcgggcga ggcggtcgac aacttcattg acctggtcaa gtcgcacctc gacgccggcg
     8461 acatcatcat cgacggcggc aattcgctct tcaccgacac catccggcgc acccggaaac
     8521 tggagtccga gggcttccgc tacatcggaa ccggggtctc cggcggcgag gagggcgccc
     8581 tgcacggtcc ggccatcatg cccggcggtt cggcgagcgc ctggccggaa gtccaggcca
     8641 tcttcgagaa gatcgcggcc aaggccccgg ccgacggcaa gccctgctgc acctggatcg
     8701 ggcccgacgg cgccggccac cacgtgaaga tggtccacaa cggcatcgag tacggcgaca
     8761 tggagttgat cgtcgaggcc tactacatca tgcgctatgt cctgggcatc ccggccgccg
     8821 agcagtcgga gatcttcaag aaatggaacc aggacgtgct cagctcctac ctgaacgaga
     8881 tcgccgggga catcctgggc cgggttgatc cggataccgg ccggccgctg gtcgaggtga
     8941 tcctggacaa ggccggccag aagggaaccg gcaagtggac ttcgcagaac gcgctcgacc
     9001 tgggcgtgcc ggccccgacc atcgccgagg cggtcttcgc ccgctgcatg tcggcgctca
     9061 aggacgagcg ggtcaaggcg gccgccatcc tgcccggccc gaaggcgaag ttcaccggtg
     9121 ataaggcggc ttatgtcgag gcgatccacg ccgccctcta ctgctccaag atctgctcct
     9181 acgcccaggg cttcggactg atgcgcgagg ccgcccggga gtacaagtgg aacctcgatt
     9241 tcgggaagat ttccctcatc tggcgcgccg gctgcatcat ccgcgcccag ttcctgcaga
     9301 agatagccga cgcttacggc cgcaacccgg agctggccaa cctcctcctg gacgagtact
     9361 tccagaagag catcgccgaa cggcaggaag cctggcggca ggtggtctcc gacgcggccc
     9421 ggctcggcat accggccccg gccttcgcct cggccctggc ctactatgac ggttatcgca
     9481 gcgaggtcct gccggccaac ctcatccagg cccagcgcga ctatttcggc gcccacacct
     9541 acgaacgggt ggaccggccc gggacttacc acaccaactg ggtggaggaa aaataatgtt
     9601 tgacgtcaag ggcaaggtcg cggtagtcac cggcgccggc ggagtgctct gcggcggcat
     9661 ggccgcctta ctggccgagc acggggtccg ggtggcggtg ctggacatct tcgaagacaa
     9721 ggcggccgcc gtggcggccg ccatccggtc cgccggcggc caggccatct cctgcaaggc
     9781 cgatgtcctg agcaaggcca gcctggagga ggcctgccgg accgtgctgg ccgagttcgg
     9841 cacggtggac atcctggtca acggcgccgg cggcaacaag aaggaggcga ccacctcgcc
     9901 ggacctttcc ttcttcgacc tgccggccga cgccttccag tgggtattca acctcaactt
     9961 catcgggtcg gtcctgccct cccaggtctt cgggcgggtg atggccgaaa agggcggcgg
    10021 cagcatcctg aacatctcct cgatgaactc cttccggccg ctgaccagga tcgtcgccta
    10081 ttcggccgcc aaggccgcca tcaacaattt cacccagtgg ctggcggtct acctggccca
    10141 gaactgctcg ccgcgcatcc gggtcaacgc catcgcgccc ggtttcttcc tgaccgagca
    10201 gaaccggttc ctcctcaccg acgagaagac cggggagctc accccgcgcg ggcagacgat
    10261 catcggccac actccgatgg gacgcttcgg agaaccggcc gatctcaacg ggaccatcct
    10321 ctggctgctg tcggacgcct cgagcttcgt gaccgggatc gtggtcccgg ttgacggcgg
    10381 cttctcggcc ttttccggag tttgaacgca cgatcaacct ggagagcaag catggatctc
    10441 aagaattttc cgaagcgttt cgattggttc gtcggcatcg attccgacgg ctgcgtcttc
    10501 ccttccatgg acatcaagca gaaagagtgt ttccacccgg cgatcatgga ccagtggggc
    10561 ctctggaagg tggagaagga agtccggatg gtcgccgaat gggtcaacct ttactcggtc
    10621 catcgcggcg tcaaccgttt tccgggtctc cggatgacct ttgatttcct gcgcgaaatg
    10681 gattcggtgc gggccgccgg gatcgcgatc cgggagaccg gcgacctgga ggcttacatc
    10741 aagagcggcc tgcccctggg caacgacacc ctcaagacct acgtccagga gcatccttcg
    10801 ctggccgacg tcctggaatg gagcctggag gtcaaccgcc gggtggcggc gaccgtcaag
    10861 gaggtcccgc ccttcccgtt cgtgcgcgaa tctctcgaga agctgcgccc cagggtggac
    10921 atgatcgtcg tgtcccagac gccgaccgag gcgctggtcc gggaatggaa ggagcacgcc
    10981 atcgacggcc tggtcgattt catcgccggg caggagcagg gcagcaagaa ggagcacctc
    11041 cagatggcgg ccgcacccaa ctacccggcc gaccggatcc tgatgatcgg cgacgccctg
    11101 ggcgacttga aggcggtcga ggcggtcggc ggtttcttct tccccatcaa ccccggtcac
    11161 gaagccgaat cgtgggagaa tttttaccag gagggaatcg agcggtttct cgccgggagc
    11221 ttcaaggggg cctaccagga gaagctgatg gccgccttca aggccctgct tccggagcag
    11281 ccgcactgga agtaagcggc cgatggtccg attgagttcc cgggagcgcc ttacgaggac
    11341 cctggccggc gaaccggtgg accgggtcgg gatttcgctc tacgagttcg acggccacta
    11401 cgagtcctgg atccaccggt tcccggagta ccaggcgctc ctggattacg cggccggccg
    11461 gaccgaccgg ctcttcttct gggcgccggc gtccccgtcg ccggcgctct tctacggcca
    11521 ggtcgaaccg gacacggtcc
//
LOCUS       DAOQYN010000034        11457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_6101_length_11457_cov_4.957902, whole genome shotgun sequence.
ACCESSION   DAOQYN010000034 DAOQYN010000000
VERSION     DAOQYN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6101_length_11457_cov_4.957902"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PKN80_02480"
     CDS             <1..421
                     /locus_tag="PKN80_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34911.1"
                     /translation="GEGNAAALAEQLKQESERAEKFRTATGQDLADFQKGLTARLDLI
                     QGDLAVLKGIQEKQGIRLAEVTGAVERLARDQAELDSQPVSFDTALVMSRPEIEARLA
                     VILEEVTKENSALRDRLTELEKRVKALKPAASSRTRR"
     gene            461..1627
                     /locus_tag="PKN80_02485"
     CDS             461..1627
                     /locus_tag="PKN80_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ34912.1"
                     /translation="MKKSLKVCHIITRLIIGGAQENTLFTCYGLLERGYEVTLLTGPP
                     LGPEGSLLAEARARGLKVVIIPALRRRIDPGRDLLALLDIYRRLLFGNFRIVHTHSAK
                     AGILGRLAAGLLPRRVRIVHSIHGLPFHPYQDRRLNFMYILAEKLVAPVTDLFLSVAD
                     AMNRQSLAAGIGRPEKYRTVYSGFAVSSFAGCRALRSAARGRWGFRESDFVIGKVARL
                     FQLKGHDLLLPVFAEARREFPDLRLFLVGDGILKPELEAEAERLGIRPSVVFAGLLPP
                     GEIPEAMAAMDMLVHVSLREGLPRAVSQALAAGLPVAAFDVDGTREVVRSGVTGYLVT
                     PGDRAGLKRAVLEIRRDPDGARRLARTGQAFVLDNFSIERMVSGVAEAYRDLTS"
     gene            1639..2535
                     /locus_tag="PKN80_02490"
     CDS             1639..2535
                     /locus_tag="PKN80_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNQ34913.1"
                     /translation="MAEIELSREYARPLAEFQEYLRSERNASGHTLAAYGSDLRDFFR
                     FLDGRPLASVRIPVLRAYLGDLFGRKLGRATLIRRVAALRTFFRFLSRRGAVEVNPAA
                     GLRSPRSERRLPVFLEIEAACHLLDLALKSAGRFPKRDLALLELLYSTGVRVSELVGL
                     DINAVDFVAGLVSVKGKGRRERLVPVGDRALRVLNDYLASPERPAAARALFTNRFGGR
                     LTGRSVERLVKKYCLLAGLPSRVTPHTLRHSFATHLLERGADLRAIQEILGHRNITTT
                     QVYTHLTTRTLKEAYRNFFPRA"
     gene            complement(2498..3070)
                     /gene="gmk"
                     /locus_tag="PKN80_02495"
     CDS             complement(2498..3070)
                     /gene="gmk"
                     /locus_tag="PKN80_02495"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNQ34914.1"
                     /translation="MILVLSAPSGTGKTTVLKILQQKYRLPRVITCTTRPPRNGETPG
                     QDYLFVDEKRFLGMMEEGELAEWATVYGHRYGTPKSAIEDRLKAGEPAILALDTQGAA
                     SIKALYPETIRVALLPPSRSELAARINQRGRASGETPATIAARLAESEKELASLRDYD
                     HRIINDDPERAADRLYRIIRRAEKNFDRPL"
     gene            complement(3067..3768)
                     /locus_tag="PKN80_02500"
     CDS             complement(3067..3768)
                     /locus_tag="PKN80_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34915.1"
                     /translation="MSKTAFRSRSIAAIILLFLILSPLQDSIDLQRRRMGFERRLMLM
                     PGQVAGNLILGGFKGLAADLLWLQVESLFHSGQHYKMLPLFQSITFLQPKFITPWAVG
                     GWHMAYNISVKAKNEEEKQFWIKHGVDFLAEGIKNNPERYDLYFELGWTYFNKVKDYA
                     NAVKYFELAAKFPRPEYVDDVLAHAYEENGQIKEAIATWERILAGPDTPFRQIAARML
                     SRLKKYGTTKVETYQ"
     gene            complement(3809..4573)
                     /locus_tag="PKN80_02505"
     CDS             complement(3809..4573)
                     /locus_tag="PKN80_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ34916.1"
                     /translation="MVKALIIGLNTFRETLRLKSYQIILIFALLLLGSSQLFSFLTPE
                     EQLKMIKDVSLSGIEIFAALLAIFTAMTSLRNEFETNTISTLLSKPLRRSEFILGKFI
                     GSVLAITLSVILMAAVFSTLILIKQHSLEAGLFSATFLIWAEMLVIVALTLAVAAFAS
                     TAFTLIFVLFLFLTGHLTGYGLELAQQAKNPLLQALTKIFYKVIPNFENFAIRDEIAV
                     GVSVSLSYLLKTFGYGLIYTFIALLLCLYFFQFREV"
     gene            complement(4558..5307)
                     /locus_tag="PKN80_02510"
     CDS             complement(4558..5307)
                     /locus_tag="PKN80_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ34917.1"
                     /translation="MDEKIALKIEGLSKVYQLGRRKEVVALDNLALEVKQGEIFGLLG
                     PNGSGKTTAIKLILGLIFPSSGTIEILGQKHTEVSVKEKIGYLPENPYYYRYLTGPEI
                     LNFFGRIFSLPEETLKERRENLLQLVGLENSGHLALKHYSKGMLERIGLAVALLNDPE
                     FLILDEPTTGLDPIGSRETRNLLGRLQSEGKTIMLSSHYLSEVEKISNRVGILHQGKL
                     LALGALRDLMTEWKASDIEDLFVKVIEWSKH"
     gene            complement(5381..6679)
                     /gene="purD"
                     /locus_tag="PKN80_02515"
     CDS             complement(5381..6679)
                     /gene="purD"
                     /locus_tag="PKN80_02515"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNQ34918.1"
                     /translation="MKCLVIGSGGREHALAWKLRQSKQVSELFIAPGNGGTADFGVNL
                     EIRADDIDGLAGFARDQAIDLTVIGPEAPLAAGLADRLQAEGRPVFGPSAAAARLETS
                     KVFAKEFMHRHRIPTAEFQTADSLAEGRALLAAWKGKPAVIKADGLAAGKGVFICETE
                     AEKEAALEAILVRQEFGAAGRRVVFEEKLTGEEASLLVLTDGRSYHPLVPAQDHKKIG
                     AGETGPNTGGMGAVAPNPFLDPGLVRRVENESVIPTLAGLESEGMRYRGILYVGLILT
                     PEGPRVLEYNARFGDPETQVVLPLLENDLAELLLAAATDRLAETGPRFSGRSACSVVM
                     ASAGYPGRYRIGYRINGLAAAAARKDVLVFHAGTRLENGRPVTAGGRVLNVTGLADRL
                     PEAVKLAYRAVAEISFEGACFRADIGRKAVGRLEETAAAA"
     gene            complement(6718..7590)
                     /locus_tag="PKN80_02520"
     CDS             complement(6718..7590)
                     /locus_tag="PKN80_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNQ34919.1"
                     /translation="MPSEEFDTALQQAFTRGEPGAILLGTLSLILLVGALYALAQNLQ
                     RLVNRVPAEPPAGAAATFWPGSLVLKVIFTALPAWLLLTAGLDALCFDGKLSFNHSAA
                     RAIIFSAALQLCLVGAILAGLRRRFLGTWQLGLDAWKTGYLRAGFWTYFSILPILAVI
                     GLLQGLLQHGRLPDQEIYAVLMRIDSQGLLLLYLAVIALAAPFLEEIIFRGFLFGTLR
                     SLFGYRRAALYSSLIFASLHQSVFAFLPIFCLALALCWLYEKSGSLWPSVVLHLLNNT
                     VTAVFVLLLRQAIS"
     gene            complement(7628..8722)
                     /locus_tag="PKN80_02525"
     CDS             complement(7628..8722)
                     /locus_tag="PKN80_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNQ34920.1"
                     /translation="MMTLKDVAQECVRLRATDIHVKIGKPPIFRINNSLIFSQLEPVT
                     PEHMESFLKEVAKESQLRKLEEERELDFGFTFGDLGRFRINVYFQRGNSSMAMRRIRT
                     KIPKFQELSLPPVLEQISKLESGLVLVAGPTSSGKSTTVASIVDFINQNEQVNIVTVE
                     DPIEYVFTDQKSIVNQREVGSDTLSFHNALKHVLREDPDIIVIGEMRDTVTFESALSA
                     SETGHLVLSTVHALDTISIITRILDFFPSNLHEQVRKQLAYHIKATVCQKLLPRKDGK
                     GLIPAVEVMLATPTIVNLIIENRVLKIPAGMRAEKDRGMQTFNDAILQLLKDDRITES
                     IGFAASPNPDALRMNIQGIFLDEDTRIIGM"
     gene            complement(8759..9505)
                     /locus_tag="PKN80_02530"
     CDS             complement(8759..9505)
                     /locus_tag="PKN80_02530"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNQ34921.1"
                     /translation="MNLNRVILLWIAALAVGWLAPGRSGGQPVSGLEDLVLREEYRAA
                     IQTYGEIPDLTPMQKYLLAVCYQETRDVKKAEEIWKNLLGSRERERSLLAIARLKGRT
                     GPTLESEQTFRQFLREYPLSTHLPTACLGLAEVLVKRGKKTEALTLLNRIRQEYPFSP
                     EAVAAGGLLNRELGEYTIQVGSFSDYLRAQKLVDDLAADGYDAYLVRIPDQAALSYRV
                     RVGNFSSRAAADRIGRTLKQANRLEYFITH"
     gene            complement(9505..9714)
                     /locus_tag="PKN80_02535"
     CDS             complement(9505..9714)
                     /locus_tag="PKN80_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34922.1"
                     /translation="MGYEALKVLDKTANQQNTYQLVAELTEKAHRLSHERISLGRSNP
                     LEEVFQAIIEREAVPAEEEAEKEKD"
     gene            complement(9803..10591)
                     /locus_tag="PKN80_02540"
     CDS             complement(9803..10591)
                     /locus_tag="PKN80_02540"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNQ34923.1"
                     /translation="MKFFILTDLEGTAGVTTWAQVLGENKEKHLQAQNWLTGEVNAAI
                     EGILAVDADADIVVWDGHGNGGIILDQLRPEARFIPRGFIRCPYGLDQGYDAMLMVAQ
                     HAKAGTPKANLCHTYSLSIYRYWLNGEEVGEMGLRSYIAGYHKVPVILATGDDAACRE
                     AAGLIPGIETVQVKEALHQELAVTLQPARARQLIKAGAERAVRRIKEIKPVYPKPPYR
                     FRVQGLNSTFAEQTLFNHSGFRRVDSCTIETESDDLQKAVEVFA"
     gene            complement(10611..>11457)
                     /locus_tag="PKN80_02545"
     CDS             complement(10611..>11457)
                     /locus_tag="PKN80_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ34924.1"
                     /translation="FWRRPRVVPPAAKPGKALTMIDLTGLARDRGLWACPAEGDLPRL
                     LERVARGYPAVALLNFGVGPWSIPHYVVVTGYDLKRPVIYFHSTWRRDSFLSPKSFQR
                     FWKRGGSLWLTMAPPDEIRFPLSPEEANQLGVFHLETGRPEAAAAAFQQALAGREAYL
                     YRFNLAVALERSGRKDEAERTYRQVLAVHPFGPAFNNLAELRLKNDPGEAEKLARQAL
                     ENDPDRRAYYLDTLGLALLKGGQPEAAREQLEMALAAAERDYPELAGLIRGHLEELKA
                     KHPSN"
BASE COUNT         1923 a         3514 c         3817 g         2203 t
ORIGIN      
        1 cggcgagggg aacgcggcgg cgctggccga gcagttgaag caggagtccg aacgggccga
       61 gaagttccgg accgcgaccg gccaggacct ggccgatttt cagaagggat tgaccgcccg
      121 gctggatctg atccagggcg acctggccgt tttgaaggga attcaggaga agcagggtat
      181 ccggttggcc gaagtgaccg gcgcggttga gcggctggcc cgggaccagg cggaacttga
      241 cagccagccg gtaagcttcg acaccgccct ggtcatgtcg cgcccggaga ttgaggcccg
      301 gttggcggtg atcctggagg aagtgaccaa ggaaaactcg gcgctgcggg accggttgac
      361 cgaactggag aagcgggtga aggccctcaa gccggccgct tcctcccgga cccgcagata
      421 aaggcccggc gggccgaggc cgatgaccgg tcagccgtcg atgaagaaat ccttgaaggt
      481 ctgccatatc atcacccggc tgatcatcgg cggcgcccag gagaacaccc tctttacctg
      541 ttacggcctc ctggagcgcg gttacgaggt gaccctcctg accgggccgc cgctcggccc
      601 ggagggcagc ctgctggccg aggcccgggc gcgcggcctc aaggtcgtta tcattccggc
      661 cctgcggcgt cggatcgacc cgggacgcga cctgctggcc ctgctggata tttaccgccg
      721 ccttctcttt ggaaacttcc ggattgttca cacccacagc gccaaggccg gaatcctggg
      781 gcgtctggcc gcgggactac tgccgcggcg ggtccggatc gtccactcca tccacggcct
      841 gcccttccat ccttaccagg accgtcgttt aaatttcatg tatatcctgg ccgagaaact
      901 ggtggcgccc gttaccgatc tctttctctc ggtggccgac gccatgaacc ggcagagcct
      961 ggccgccggc atcggccgtc cggaaaagta ccggacggtc tacagcggtt tcgcggtgtc
     1021 atcctttgcc ggctgtcggg cgctccggtc ggcggcccgg ggtcgttggg gatttcggga
     1081 gagcgatttc gtaatcggca aagtggcccg gctctttcag ctcaagggac acgacctgct
     1141 gctgccggtc tttgccgaag cgcggcggga gtttcccgac ctgcgcctct tcctggtcgg
     1201 cgacggcatc ctgaagccgg aactggaagc cgaggccgaa cggctcggta tccgcccgtc
     1261 ggtggtcttt gccggcctgc tgccgcccgg agagattccc gaggcgatgg cggccatgga
     1321 catgctggtc cacgtttccc tgcgcgaggg tttgccgcgg gcggtttccc aggcactggc
     1381 cgccggcctg ccggtggcgg cctttgatgt ggacggcacc cgcgaagtgg tgcgttccgg
     1441 cgtgaccgga tacctggtga cgcccggcga ccgcgcgggg ctcaagcggg cggtcctgga
     1501 aatcaggcgc gatccggacg gcgcccgccg cctggcccgg accggccagg ccttcgtgct
     1561 tgacaacttt tccatcgagc ggatggtctc gggcgtggcg gaggcctacc gtgatctgac
     1621 cagctgagga aacgctccat ggcggaaatc gaactttccc gggagtacgc gcggccgctg
     1681 gccgaattcc aggagtacct gcgcagcgag cggaacgcct ccggccacac cctggccgcc
     1741 tacggctccg acctgcgcga tttcttccgt ttcctggacg gccggcccct ggcctcggtc
     1801 cggatcccgg tcctgcgcgc ctacctgggc gatctcttcg gccgcaagct tggccgggcc
     1861 accctgatcc gccgggtggc cgccctgcgc accttcttcc ggttcctgag ccgccggggc
     1921 gcggttgaag tcaatccggc cgccggcctg cgttcgccca ggagcgaacg cagactgccg
     1981 gtcttcctgg aaatcgaggc cgcctgtcat ctgctggatc tggccctgaa aagcgccggc
     2041 cgctttccga agcgggacct ggctctcctg gagctgcttt attcgaccgg ggtgcgcgtt
     2101 tcagaactgg tgggcctgga catcaacgcg gtcgatttcg tggccggtct ggtgagcgtc
     2161 aaaggaaagg ggcggcggga aaggctggta ccggtgggcg accgggccct tcgggtcctg
     2221 aacgattacc tggcttcacc ggaacggccg gccgcggccc gggcgctctt caccaaccgg
     2281 ttcggcggcc ggctgaccgg ccgcagcgtg gaacggctgg tcaagaaata ctgcctgctg
     2341 gccgggctgc cgtcgcgggt gacgccgcac accctgcgcc actcgtttgc cacccacctg
     2401 ctggaacggg gcgccgacct gcgcgccatc caggagatct tgggccaccg caacatcacc
     2461 accacccagg tttatactca cctgaccacc cggacgctca aagaggccta tcgaaatttt
     2521 tttccgcgcg cctgatgatc cggtaaagcc gatcggcggc ccgttccgga tcgtcattga
     2581 tgatccggtg gtcataatcc cgaaggctgg ccagctcctt ttcactttca gccagccggg
     2641 ccgcgatcgt cgccggcgtc tcccccgagg cgcggccgcg ctggttgatc cgggcggcca
     2701 gttccgaccg ggagggtggc agcaacgcga cccggattgt ctccgggtaa agggccttga
     2761 tgctggccgc gccctgggtg tccagtgcca ggatggccgg ttcgcccgcc ttcagccggt
     2821 cttcgatggc cgatttcggc gtgccgtagc ggtggccgta aacggtggcc cactcggcca
     2881 gttccccctc ttccatcatc ccgaggaaac gcttttcatc cacgaagaga taatcctgtc
     2941 ccggggtttc gccgttgcgc ggcggccggg tggtgcaggt gatgacgcgg ggcaggcggt
     3001 acttctgctg gagaatcttg aggacggtgg tcttgccggt tccggaaggg gcggacagga
     3061 ccaggatcat tgataggttt cgaccttggt cgtcccgtat tttttgaggc gggagagcat
     3121 gcgggcggct atctgccgga acggggtatc cggcccggcc aggattcttt cccaggtggc
     3181 gatggcctcc ttgatctggc cgttttcctc gtaggcgtgg gccagcacgt cgtccacgta
     3241 ttcgggccgc ggaaacttgg cggccagttc gaagtacttg accgcgttgg cgtagtcctt
     3301 gaccttgttg aagtaagtcc agcccagttc gaagtagagg tcgtagcgtt cggggttatt
     3361 cttgatgcct tcggccagga agtccacgcc gtgcttgatc cagaactgct tctcctcctc
     3421 gttcttggcc ttgaccgaaa tattgtaggc catgtgccag ccgcccaccg cccagggggt
     3481 gatgaacttg ggctggagga aggtgatgga ctggaagagc ggcagcatct tgtaatgctg
     3541 gccggagtgg aagaggcttt cgacctggag ccagagcagg tcggcggcca ggcccttgaa
     3601 gccgccgagg atgaggttgc ccgccacctg gccgggcatg agcatcagcc gccgctcgaa
     3661 gcccatcctc cggcgctgca ggtcgatgga gtcctggagc ggcgacagga tcaggaagag
     3721 caggatgatg gcggcgatgc tccggctgcg aaaggcggtc ttggacattt ttcctggcga
     3781 tggcgacaag aagcggcgcg gggcggcctc agacctctct gaactggaag aagtagaggc
     3841 agagcagcag ggcgatgaag gtgtagatca gcccgtagcc gaaggttttc aagaggtaag
     3901 agagcgacac cgacactccc accgcgatct catcgcggat cgcgaagttt tcgaagttcg
     3961 ggatgacttt gtaaaagatc ttggtcaggg cctgcagcag gggattcttg gcctgctggg
     4021 ccagctccag gccgtagccg gtcaggtggc cggtcaggaa gaggaagagg acgaagatca
     4081 gggtgaaggc ggtcgaggcg aaggcggcca ccgccagggt cagggcgacg atgaccagca
     4141 tctcggccca gatcaggaag gtggccgaga agaggccggc ctccagggaa tgttgtttga
     4201 tcaggatcag ggtcgagaag accgccgcca tcaggatgac gctcagggtg attgccagga
     4261 ccgaaccgat gaacttgccc aggatgaact cggagcggcg cagcggcttg gagagcaggg
     4321 tggaaatggt gttggtctcg aactcgttgc gcaggctggt catggcggtg aaaatggcca
     4381 gcagcgcggc gaatatctcg atcccggaaa gggaaacgtc cttgatcatc ttcagctgct
     4441 cctccggggt gaggaaggag aagagctggc tgctgcccag caggagcagg gcgaagatca
     4501 ggatgatctg ataactcttc agccgcaggg tttcccggaa agtattgagc ccgataatca
     4561 gtgctttgac cattcgatca cctttacgaa caagtcttca atatcgctgg ccttccattc
     4621 cgtcatcaga tcccgcaggg cgcccagggc cagaagtttt ccctggtgca ggattccgac
     4681 gcggttgctt atcttttcga cctcggaaag gtagtggctg gagagcatga tggtcttgcc
     4741 ttcggactgc agccggccca gcaggttgcg ggtttcgcgg ctgccgatcg ggtcgagtcc
     4801 ggtggtcggt tcgtcgagaa tgaggaactc cgggtcgttg agcagggcga ccgccaggcc
     4861 gatcctttcc agcatgccct tggaatagtg cttcagggcc aggtggccgg aattctccag
     4921 tccgaccagc tgcagcaggt tttcccggcg ctctttcaga gtctcctcgg ggagggagaa
     4981 gatccggccg aagaagttca ggatctccgg tccggtcagg tagcggtaat aataaggatt
     5041 ctcaggcagg tagccgatct tctccttgac cgagacctcg gtatgcttct gaccgaggat
     5101 ctcgatggtg ccgctgctgg gaaagatcag cccgagaatg agcttgatgg cggtggtttt
     5161 gccgctgccg ttgggtccca gcagtccgaa gatctcgcct tgcttgacct ccagcgccag
     5221 gttgtccagc gccaccacct ccttgcggcg gccgagctga tagaccttgc tcaatccctc
     5281 gattttgagt gcgatttttt catccataat ggttaattgt agcaaaagag acgctttcaa
     5341 gtcaaggcag aattaacggg tccgtccggg gtttgccgtc tcaagcggcg gcggctgttt
     5401 cttccaggcg gcccacggcc ttccggccga tgtccgcgcg gaaacaggcc ccttcgaagg
     5461 agatctccgc caccgcccgg taggccagtt tgaccgcctc cggcagccgg tcggccagcc
     5521 cggtgacgtt caggacccgc cccccggcgg ttaccggccg cccgttttcc agacgggtgc
     5581 cggcgtgaaa gaccaggacg tccttccggg cggcggccgc cgccagcccg ttgatccggt
     5641 aaccgatccg gtagcggccc gggtacccgg ccgaggccat gaccaccgag caggcggaac
     5701 ggcccgagaa gcgcggaccg gtttcggcca gccggtcggt tgccgcggcc aggagcagtt
     5761 cggccaggtc gttttccagc aggggcagca ccacctgggt ttccgggtcg ccgaagcggg
     5821 cgttgtactc cagcacccgt ggtccctccg gggtgaggat caggcccacg tagagaattc
     5881 cccgatagcg cattccctcg gactccagcc cggcgagcgt ggggatgacg ctctcgtttt
     5941 caacccgtcg gaccaggccc gggtcgagaa aagggttggg cgccaccgcg cccatgccgc
     6001 cggtgttggg cccggtctcg ccggccccga tcttcttgtg gtcctgggcc ggcaccagcg
     6061 ggtggtatga ccggccgtcg gtcagcacca ggagcgaggc ctcctcgccg gtcagctttt
     6121 cctcgaagac cacccggcgg ccggccgccc cgaactcctg ccggaccagg atggcctcca
     6181 gggccgcctc cttttccgcc tcggtctcgc agatgaagac gcccttcccg gccgccaggc
     6241 cgtcggcctt gatgaccgcc ggttttccct tccaggccgc caggagcgcc cggccttcgg
     6301 cgagggagtc ggcggtctgg aactccgcgg tcggtatccg gtggcggtgc atgaattcct
     6361 tggcgaagac cttggaggtt tccagccggg cggcggcggc cgagggtccg aagaccggcc
     6421 gcccctcggc ctggagccgg tcggccaggc cggcggccag cggggcctcc ggcccgatca
     6481 cggtcaggtc gatggcctga tcccgggcga acccggccag gccgtcgatg tcgtcggccc
     6541 ggatctcgag attgactccg aaatcggccg tgccgccgtt gccgggggcg atgaagagtt
     6601 cggagacctg tttgctctgc cgcagtttcc aggccagggc gtgttcccgg ccgccggagc
     6661 cgattaccag gcatttcatg ttttaccccg cagaagatgg cgttttccgg cggcccttca
     6721 ggagatggcc tgccggagca gaaggacgaa gacggccgtg acggtgttgt tgagcaggtg
     6781 aagcaccacc gacggccaga gcgagccgct cttttcgtag agccagcaga gcgccagcgc
     6841 caggcagaag atgggcagaa aggcaaagac cgactgatgc agcgaagcga agataaggct
     6901 ggagtacaac gcggcgcgcc ggtaaccgaa gagggagcgc agggttccga agaggaagcc
     6961 ccggaagatg atctcctcca ggaagggggc ggccagggcg atgactgcca ggtagagcag
     7021 gagcaggccc tgcgagtcga tccgcatcag gaccgcgtaa atctcttggt cgggcagccg
     7081 gccgtgttgc aggaggccct ggaggagtcc gatgacggcc aggatcggca ggatggagaa
     7141 ataggtccag aagccggccc ggagataccc ggttttccag gcgtccaggc cgagctgcca
     7201 ggtccccagg aaacggcggc gcagtccggc cagaatggcc cccaccaggc agagctggag
     7261 ggcggcgctg aagatgatgg cccgggcggc gctgtggttg aaggagagct tgccgtcgaa
     7321 gcagagggcg tcgaggccgg cggtgagcag cagccaggcg ggcagcgcgg tgaaaatgac
     7381 cttcagcacc aggcttcccg gccagaaggt ggcggccgcg cccgccggcg gctcggccgg
     7441 gacccggtta accaggcgct gcaggttctg ggccagcgcg tagagggcgc cgaccaggag
     7501 gatcagggaa agcgtcccca gcaggatcgc cccgggctcg ccccgggtga aggcctgctg
     7561 gagggcggtg tcgaattctt cggacggcat cgtgtttatt ttggccggtt ccggcccttc
     7621 aggacgccta catcccgatg atccgggtat cctcgtccag gaagatgccc tgaatattca
     7681 tgcggagcgc gtccgggttg ggactggcgg cgaaaccgat cgactcggtg atccggtcgt
     7741 ccttgagcag ctggagtatg gcatcgttga aagtctgcat accccggtcc ttttccgccc
     7801 gcattccggc cgggatcttg agcacccggt tctcgatgat caggttgacg atggtgggcg
     7861 tggccagcat cacctcgacc gccggtatca aacccttgcc gtccttgcgc ggaagcagtt
     7921 tctggcagac ggtcgccttg atgtggtagg ccagctgttt gcgcacctgc tcgtgcaggt
     7981 tgctgggaaa gaaatcgagg atgcgggtaa tgatgctgat cgtgtccagg gcgtggacgg
     8041 tcgaaaggac caggtggccg gtctccgaag ccgagagggc cgattcaaag gtcaccgtgt
     8101 cgcgcatttc gccgatgacg atgatgtccg ggtcctcgcg cagcacgtgt ttcagcgcat
     8161 tgtggaagga gagggtgtcg ctgccgacct cgcgctggtt cacgattgat ttctggtcgg
     8221 tgaagacata ctcgatcggg tcctccaccg tcacgatgtt gacctgttcg ttctggttga
     8281 tgaaatcgac gatcgaagcc accgtggttg actttcccga actggtcggg ccggccacca
     8341 ggaccaggcc gctttccaac ttggagatct gttccaggac cggcggcagg gagagctctt
     8401 ggaactttgg gatcttggtc cggatgcggc gcatggccat actggaattg ccgcgctgaa
     8461 agtagacgtt gatccggaat cggcccaggt cgccgaaggt aaatccgaaa tccagctccc
     8521 gttcctcctc cagtttccgc agctgggact ccttggcgac ctccttcagg aagctttcca
     8581 tgtgctccgg agtcaccggc tccagctggg agaagatcag gctgttgttg atccggaaga
     8641 tcggcggctt cccgatcttg acgtggatgt cggtggcccg cagccttacg cattcctggg
     8701 cgacatcttt caaggtcatc atgtttcccc tgcctgttcc ggcccgcctc cgggcctctt
     8761 aatgggtgat gaaatactcc agacggttgg cctgtttcag ggtgcggccg atgcggtcgg
     8821 cggcggcccg ggaagaaaaa ttgccgaccc gcacccggta ggagagggcg gcctggtcgg
     8881 gaatgcgcac cagataggcg tcataaccgt ccgcggccag gtcgtcgacc agtttttggg
     8941 cgcgcaggta gtcgctgaag gagccgacct ggatggtgta ctctcccagt tcgcggttca
     9001 gcaggccgcc ggcggcgacc gcttccgggc tgaacggata ctcctgccgg attcggttca
     9061 gcagcgtcaa cgcttcggtc ttcttgccgc gtttcaccag gacctcggcc agccccaggc
     9121 aggcggtcgg caggtgagtg ctgagcggat actcccgcag aaactggcgg aaggtctgct
     9181 cactctccag cgtcgggccg gtccgccctt tcagccgggc gatggccagc aggcttcgtt
     9241 ccctttcccg gctgcccagc aggtttttcc agatttcctc ggccttcttg acgtcccggg
     9301 tctcctggta gcagacggcc agcaggtact tctgcatcgg ggtcaggtcg gggatttcgc
     9361 cgtaagtttg gatggccgcc cggtattctt cgcgcaggac caggtcttcc agtccggaga
     9421 ccggctgacc tccggaccgt ccgggagcta accacccgac ggccagcgcc gcgatccaca
     9481 ggagtatcac ccgattgagg ttcatcaatc cttttctttt tccgcttctt cttccgcggg
     9541 gaccgcttcc cgctcgatga tcgcctggaa gacctcctcc agaggatttg acctgcccag
     9601 cgatatccgt tcgtggctta accggtgggc cttttcggtc aattcggcca ccaattgata
     9661 ggtattctgc tggttggcgg ttttatccag gaccttcaac gcttcgtatc ccatggtctg
     9721 tcctctcgac ggttgggttt tttccgaatg gcaacggtta ttataccctc ttcaaaccgg
     9781 gtgcggcaaa ggcggttccc ggtcaggcaa agacctcgac ggccttctgg aggtcgtcac
     9841 tctcggtttc gatcgtgcag ctgtccaccc ggcggaatcc ggaatggttg aaaagcgtct
     9901 gttcggcgaa ggtcgaattc aggccctgga cccggaaccg atagggcggc ttgggataga
     9961 ccggcttgat ttccttgatt cgtctgacgg cccgttcggc gccggccttg atcaactggc
    10021 gggcccgcgc cggctgaagc gtaacagcca gttcctggtg cagggcttcc ttgacctgga
    10081 ccgtttcgat gcccgggatc aggccggcgg cctcgcggca ggcggcgtcg tcgccggtgg
    10141 ccaggatgac cgggaccttg tggtaaccgg cgatgtaact gcgcagtccc atttcgccca
    10201 cctcctcgcc gttcagccag taacggtaga tcgacagcga ataagtgtgg cagaggttgg
    10261 ctttcggcgt gccggccttg gcgtgctggg cgaccataag catcgcgtcg tatccctggt
    10321 caagtccgta ggggcagcgg atgaagcccc tcggtatgaa gcgggcttcg ggtcggagct
    10381 ggtcgagtat gatgccgccg ttgccgtgtc cgtcccagac cacgatatcg gcatcggcat
    10441 ccacggccag gattccctcg atggcggcat tgacctctcc ggtcagccag ttttgagcct
    10501 gaaggtgctt ctccttgttc tcccccaaga cctgggccca ggtggtgacc ccggcggttc
    10561 cttccaggtc ggtcagtatg aagaatttca tttcgtctcc tttcccccgt tcagtttgaa
    10621 ggatgcttcg ctttaagttc ttccaggtgg ccgcggatca gtccggccag ttccgggtaa
    10681 tcccgttcgg ccgcggccag tgccatctcc aattgctcgc gggccgcctc cggttggccc
    10741 cctttcaaga gggccaaccc cagcgtgtca aggtaatagg ctcggcggtc cggatcgttt
    10801 tccagtgcct ggcgggccag tttttcggcc tcgccgggat cgtttttcag ccgcagttcg
    10861 gccaggttgt tgaaggccgg gccgaaggga tggacggcca gcacctgccg gtaggtccgt
    10921 tccgcctcat ccttccgtcc gcttcgttcc agggccaccg ccaggttgaa ccggtagagg
    10981 taggcctccc ggccggccag ggcttgctga aaggccgccg cggccgcttc aggccggccc
    11041 gtttccaggt gaaagacccc gagctggttg gcctcctccg gagacagcgg aaaccggatt
    11101 tcgtccggcg gcgccatggt cagccagagt gaaccgcccc gtttccagaa gcgttgaaaa
    11161 ctcttgggtg agaggaagga gtcccggcgc caggtggagt ggaagtaaat caccggccgt
    11221 ttcaagtcgt agccggtcac gaccacgtag tgcggtattg accaggggcc gaccccgaag
    11281 ttcaacaggg ccacggccgg gtagccccgg gccacccttt ccagcagcct cggaagatcg
    11341 ccctcggccg ggcaggccca gaggccgcga tctctggcca gtccggtcag gtcgatcatg
    11401 gtcaacgctt ttccgggctt ggccgccggc ggaacgaccc gcggccggcg ccagaaa
//
LOCUS       DAOQYN010000035        11405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_6130_length_11405_cov_4.599912, whole genome shotgun sequence.
ACCESSION   DAOQYN010000035 DAOQYN010000000
VERSION     DAOQYN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11405
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6130_length_11405_cov_4.599912"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PKN80_02550"
     CDS             complement(<1..1030)
                     /locus_tag="PKN80_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34925.1"
                     /translation="MLPRGHLRSGYRSPRLFTLLLLFFCLFLPVPVYSGPSLLAEHAY
                     LRKVHQFDRGAVHVFLKNPGAAPVEVEEVYLNGVRLENLPDETAAWTQVLPSPVPPGE
                     ISDLTIMLSQNAANYKLPLEIKVKLQTGETLVIPVDRINGPFKITGIYFPDHLDQVYI
                     YLQNTGQTTLTLHKVWLSGRDVTGQTRFLARALPAGEKSLLRVDLPQPLSRGKYVSVR
                     LETADGSVGQALVRVFSVFPITSYSNPDTRPELYFDPQPFHVKYKPAVSEAEIKSLPA
                     QSAWYLFSCPNCTDEDQCKPWGNSAIEIIKRAQEFYRLDPSRPNHTHVCEAYKERAYF
                     IYGEVCDIM"
     gene            1237..2337
                     /locus_tag="PKN80_02555"
     CDS             1237..2337
                     /locus_tag="PKN80_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNQ34926.1"
                     /translation="MKRIDLYLGREFIKTYLMAVAAVMVIGLAGAMVDDLADYLRDGY
                     TLAATLRAFLWMVPAIFVQFSPLAVMITVLVVLGTMSRKQEIMILEGAGLHPFRFLVP
                     FLVLGLPLSLAGLAVNEGLVPKSLGQVRSQPVLTRPTLALPNLFMYADNYQVRERTFT
                     QINLLRFGPAGTLEELVKARRARVGKSGWDFETGTRLTFAPDGGVAGETPFQTQRLDL
                     DLNVQQLDSLLRPVEVLSLRQLSDYLRRLAPADLAPATIRTAYYGRFSYPALNLVLVL
                     LALPLLLGRHLNRSFLILIGIILSILTYWLYSFCLALGKQASLPPLLAAFLPHLVIIA
                     AAFLYAWLSAFSRDRFRRRAAVCPVQPDSGKI"
     gene            2434..4881
                     /locus_tag="PKN80_02560"
     CDS             2434..4881
                     /locus_tag="PKN80_02560"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ34927.1"
                     /translation="MNHLEKLELKPEQLRLVCRPDECPCTSTREITPLEGVIGQDRAR
                     RAIDLGLNIQKFGFNIYVSGLPGTGRSTSVDRAVADLAAQVPVPDDWCYVYNFAEPDR
                     PNALRLPPGKGREFQRDLKNLLEELKVEVPRSFESKEYEEQRNEIMHYLQEERQKALT
                     ALQEKVAGEGFTLKQTPTGLALLPSQDGRPLSDAEYEKLGPTEKDFFRQKQEKLRHDV
                     SAMIRQVRDLEKEAKELVHKLDLDTGNYLLNHHFQELRQKYEALPEVAAHLDQVRQQL
                     LDHIHDFMEKEEAEVMPGLKLPTQAPSFLPYQVNLLVDNSQTQGAPVVWESNPTYFNL
                     VGRQEYRPHLGSFLTDFTLIKAGALHRANGGFLILQANDLFNNYYSWAALKRCLKNRA
                     IRIEDLAEQFHLISTTVSRPEPIPLETKVILIGNPYIYQMLFAWDEDFSRLFKVKADF
                     DHRIERSPGGIRQYAAFVSRHCGEKNLLPFDCSALGKIVEYGSRLVADQKRLSSQFAS
                     IVAILEEADYWARQASTDRVGGAEVRRAIEERVYRNNRIEERLREMVADGQILIDDRG
                     AVVGQINGLAVLTLGDYAFGRPSRITVRTFVGKEGILDVERETKMSGPIHTKGVLILS
                     GFLGERYAHTEPLTLSASICFEQSYEEIDGDSASSTELYALLSSLAELPIRQDIAVTG
                     SVNQKGEVQAIGGVNEKIEGFFEVCKLRGLTGEQGVIIPRANIQNLMLKEEVVEAAAA
                     GRFHVWAVSSIDEGIEILTGVAAGQRRPDGSYPPNTVNGRVARKFQKLNSYYTSSQRP
                     RRPARKAAARKGPRVKK"
     gene            complement(4871..5665)
                     /gene="lpxI"
                     /locus_tag="PKN80_02565"
     CDS             complement(4871..5665)
                     /gene="lpxI"
                     /locus_tag="PKN80_02565"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNQ34928.1"
                     /translation="MKKIGLIAGNGKLPLVFLERARREDYRVYTLALKGEADRRVSSL
                     STRCLPLPIGNLQRGINFFLGNDVEEAVMIGQVRHHRLLRKERLDPLLADLLAGLPDW
                     RTGTLLQALIRQFVDRGIHFLPSTFLLDELLLPAGCHYGPTLTRREELDLILGRQVAE
                     ALALLDVGLTAVVKDGIVLALEAIEGTDRTIRRAGRFTRGAVVVKVSRPNQDMRFDLP
                     VVGPRTIRTLQNSDGRVLGISSGTLVLEKEKVAARAEALGISLVTF"
     gene            complement(5662..6453)
                     /gene="lpxA"
                     /locus_tag="PKN80_02570"
     CDS             complement(5662..6453)
                     /gene="lpxA"
                     /locus_tag="PKN80_02570"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNQ34929.1"
                     /translation="MNIHPMASVAPEAKLGRSVLIGPGAVIDGQVEIGDGTTVGPHAV
                     ITGHTTVGRDCYIYTGAVIGSPPQDLKYRGERSYLRIGDRNRIREYVTINPATENDQA
                     TIIGSDNLIMAYCHIAHNCQVGSHTVLANNATLAGHVTVEDHAILGGLTAVHQFCRIG
                     AYAMVGGYSKVIKDIPPYCLADGYPARIYRLNRVGLKRHDFSPETLATLERAYRLLVR
                     SGLNLSQAIQHIRDELPPLPEIVRLLEFINGSRRGLALSRRSRYR"
     gene            complement(6484..6744)
                     /locus_tag="PKN80_02575"
     CDS             complement(6484..6744)
                     /locus_tag="PKN80_02575"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNQ34930.1"
                     /translation="MVGLLAVYTPGFVKITRSYARLQHYRKSIPRLEKANRASKAEIE
                     LLENDPLTTERLARQELGLIRPGEWTIRFEEKKREGSAGPGH"
     gene            complement(6768..8051)
                     /gene="eno"
                     /locus_tag="PKN80_02580"
     CDS             complement(6768..8051)
                     /gene="eno"
                     /locus_tag="PKN80_02580"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNQ34931.1"
                     /translation="MACIVDVKARMILDSRGNPTVEAEVYLEDGSFGRAAVPSGASTG
                     VFEAVELRDGGKKFNGKGVARAVENVNLVIGPKVLDYEADDQIGLDRLMIELDGTPNK
                     GKLGANAILSVSMAVARAAADSHGLPLYRYLGGTLANVLPVPMMNIINGGLHADNNLD
                     IQEFMIMPAGAASFSEGYRMAAEIFQTLKKVLHEKGYSTAVGDEGGFAPSVKNNEEGL
                     KLILAAIEKAGYEPGRDVFLALDVAASSLFEKGSYAWEGRKVKFGDLIEFYQKIVKAY
                     PVISIEDGLAEDDWDGWVALTAALGGRIQLVGDDLFVTQAARLRDGIARKAANSILIK
                     LNQVGSVTETLETIETANRASYTSVISHRSGETEDTFIADLAVATGAGQIKTGSLSRS
                     ERLAKYNQLLRIEEELGGSAVYARVPAAVRGQKRK"
     gene            complement(8154..9215)
                     /locus_tag="PKN80_02585"
     CDS             complement(8154..9215)
                     /locus_tag="PKN80_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ34932.1"
                     /translation="MKDLLTRLRRERPAAFFSFWVLVLFYAAALLADLLAPYPYDFQT
                     REKPYSPPTPIRFREANGGRWHLRPFIYDQVPPEGAAIAYRADASKPYFIRLFPAGPE
                     RKIFGFLPCRRYLLGVPEPARLYLLGSDQFGRDLFSRILHGGRVSLSIGLVGVTISFL
                     LGLLFGGLAGYYGGRVDNLLMRVAELLMSFPGFYLILALRAVFPTTLSSYQVYLMVVA
                     ILALIGWAGLARVVRGMVLSIREEDYILAARALGFSNRRIIWNHVLPQTFSYTITAVS
                     LSIPGYILGESALSILGLGIQEPQASWGNILASAMGNLEVILYHAWMLLPGLLIFVTV
                     LAYNLLGDGLRELVGPSPD"
     gene            complement(9212..10210)
                     /gene="galT"
                     /locus_tag="PKN80_02590"
     CDS             complement(9212..10210)
                     /gene="galT"
                     /locus_tag="PKN80_02590"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNQ34933.1"
                     /translation="MSSRLRWDPVLEEWVVIAAHRLSRTFLPPRKWCPLCPTRAGLPA
                     TELTEPDYQVAVFENRWPSFDTRTEAGAGAAGEASGLYRERPARGICEVVCYTSDHDR
                     TLAGLSRRQLERLVEVWQDRYLCLGSKPGIKYVFIFENKGEPVGVTLHHPHGQIYAFP
                     YLPPRIKRELDSARRYHRRTGQCLFCETLARELADGRRLVAVNDTFAAFVPYAARWSY
                     EVHVYSRRHLASLAEFGPAERAGLAAILKEVTGRYDRLFDLSFPYMMVFHQAPTDGRK
                     HPGYHFHLEFYPPLRARDKLKYLAGCESGAGTFINDTWPEESAAQLRGSKAGKRRP"
     gene            complement(10207..>11405)
                     /locus_tag="PKN80_02595"
     CDS             complement(10207..>11405)
                     /locus_tag="PKN80_02595"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNQ34934.1"
                     /translation="VTVGIWARVGSRHEAPAENGASHFLEHLLFKGTARRSCEELKRL
                     VEGVGGTFNAFTGEEATCFYVKMPFARHLPLALDVLSDMVTGSRLPPEEVEKERNVIL
                     EEIKMYRDIPAAHVDELFQALVFPDHPLGRFIAGTPETIAGLSRETLSDYWDRHYRTG
                     SLVACVAGDFRVEAARELVGNYLAAVKPGPRPVFEAVRPAADRPDFSLLEKETEQAHF
                     CLGGRGCSDDNPHRWPLSVLSVILGGNMSSRLFVEVREKRGLAYQISAGHHTFRDAGI
                     FTVDAGTSPGTLKEALRVILAELARVRDELVPEEELTRARDYVTGQFQMHLEGTAEYM
                     NFLGGQLATRDRIRPPEEIIARIAAVTAEDVRRAAAAVFTGANLRLAVIGPPGSTAGL
                     RELVSL"
BASE COUNT         2107 a         3712 c         3663 g         1923 t
ORIGIN      
        1 acatgatgtc gcagacctcg ccgtagatga agtaggcccg ctccttgtac gcctcgcaga
       61 cgtgggtgtg gttcggccgg ctcgggtcca gccggtagaa ctcctgcgcc cgcttgatta
      121 tctcgatcgc cgaattgccc cagggtttgc actggtcctc gtcggtgcag ttgggacagc
      181 tgaaaaggta ccaggccgac tgggccggca gggattttat ctccgcctcg ctgacggccg
      241 gcttgtactt gacgtggaag ggctgcgggt cgaaatagag ttccggtcgg gtgtccgggt
      301 tggagtagga ggtgatcggg aaaaccgaga agacccgtac cagggcctgg ccgactgaac
      361 cgtcggcggt ttccaggcgc accgaaacgt atttgccccg ggatagcggt tggggtaaat
      421 ccacccgcag caggcttttt tcacccgccg ggagcgcccg cgccagaaac ctggtctggc
      481 cggtgacatc ccggccggac agccagacct tatgaagggt gagcgtagtt tggccggtgt
      541 tctgcaggta gatatagacc tggtcgaggt gatccggaaa gtagatgccg gtgatcttga
      601 aagggccgtt gatccggtca acgggtatca ccagggtctc cccagtttgg agctttacct
      661 ttatctccag ggggagtttg tagttggccg cgttctggga cagcatgatc gtcaggtccg
      721 aaatctcacc cggcgggacc gggctcggca aaacctgcgt ccaggcggcg gtctcgtcag
      781 gcaggttctc cagccggacg ccgttaagat agacctcctc cacctcaacc ggcgcggcgc
      841 ccgggttctt cagaaaaacg tggaccgcgc cccgatcgaa ttggtggact ttgcggaggt
      901 aggcgtgctc ggccagcagg gacggcccgg agtaaacggg caccggaagg aagaggcaga
      961 aaaacagaag aagcagggta aacaaccgag gggaccggta accggaacgc aagtgtccgc
     1021 ggggcagcat ggatcagcct ccacgagttt gcgcctgcga aaagtgattt ttaatgcctg
     1081 attgccatta tacccccccc ccggccagtt tgtcaaatca gccgccccgt tgtgctacaa
     1141 tatcttacga ctgaaggccc gcagccgatc cttcaaaatt caaaagactg gggcggccgc
     1201 gagctttctt ctccgtaaaa gccctgtcaa tttccaatga agcggatcga cctttacctg
     1261 ggccgggagt tcatcaagac ctacctgatg gcggtcgccg ccgtgatggt gatcggcctg
     1321 gccggggcca tggtcgacga cctggccgat tacctgcgcg acggctacac cctggcggcc
     1381 accctgcgcg ccttcctctg gatggtgccg gccatcttcg tccaattctc gccgctggcg
     1441 gtgatgatca ccgtcctggt cgtcctgggg acaatgtccc gcaagcagga gatcatgatc
     1501 ctggaggggg ccggtctcca tcccttccgc ttcctggtcc ccttcctggt cctgggcctg
     1561 ccgttgagcc tggccggcct ggcggtcaac gaaggcctgg ttcccaagtc gctcggccag
     1621 gtccgctccc agccggtact gacccggccg accctggccc tgccaaatct cttcatgtac
     1681 gccgacaatt accaggtccg ggagcgaacc ttcacccaga tcaacctgct ccgtttcggc
     1741 ccggccggca cgctcgagga gctggtcaag gcccgccggg cccgggtggg aaagagcggc
     1801 tgggacttcg aaaccgggac gcggctcacc tttgccccgg atggcggcgt ggccggggaa
     1861 acgcccttcc agactcagcg gctggacctg gatctcaacg tccagcagct ggattccctg
     1921 ctgcgcccgg tggaggtgct cagcctccgg cagctctcgg actacctgcg ccgcctggcg
     1981 ccggccgacc tggcgccggc caccatccgg accgcctact acggccggtt cagctacccg
     2041 gccctcaacc tggtcctggt cctgctggcc ctgccgctcc tgctcggccg ccacctgaac
     2101 cgctccttcc tgatcctgat cgggatcatc ctctccatcc tcacctactg gctctactcc
     2161 ttctgcctgg ccctgggcaa acaggccagc cttcccccgc tcctggccgc ctttctgccc
     2221 cacctggtca tcatcgccgc cgccttcctc tacgcctggc tctccgcctt cagccgggac
     2281 cgcttccggc gccgggccgc ggtttgccct gtccagccgg attctggtaa aatttaggaa
     2341 aatggaagcc cgcaccggct tccgcgcacc agtcaaaccg gacggggtcc ccggcatggt
     2401 acccgcaggt gctgacaacc tttcaaaaac aaaatgaacc acctggaaaa actggaattg
     2461 aagccggagc agctgcggct ggtctgccgg cccgacgagt gtccctgcac ctccacccgg
     2521 gagataacgc cgctggaagg ggtgatcggc caggaccggg ccaggcgcgc catcgacctc
     2581 ggcctaaaca tccagaagtt cggtttcaac atctatgtct ccggcctgcc gggcaccggc
     2641 cggtctacct cggtggaccg ggcggtggcc gacctggccg cccaggtccc ggtgccggac
     2701 gactggtgct acgtctacaa cttcgccgag ccggaccgcc cgaacgccct gcgcctgccg
     2761 ccgggcaagg gtcgggaatt ccagcgcgac ctgaagaacc tcctggaaga gctgaaggtg
     2821 gaggtcccgc gctccttcga gagcaaggag tacgaggagc agcgcaacga gatcatgcac
     2881 tacctgcagg aggagcgcca gaaggcgctg accgccctgc aggaaaaggt agcgggcgaa
     2941 ggattcaccc tcaagcagac cccgaccggg ctggccctgc tgccctccca ggacggacgc
     3001 cccctttcag acgccgagta cgagaagctg ggaccgaccg agaaggactt cttccgccag
     3061 aagcaggaga agctccgcca cgacgtctcg gcgatgatcc gccaggtgcg cgacctggag
     3121 aaggaggcca aggagctggt gcacaagctc gacctggaca ccggcaatta cctcctgaac
     3181 caccatttcc aggaattgcg ccagaagtac gaggccctgc cggaggtggc cgcccacctg
     3241 gaccaggtgc gccagcagct gctggatcat atccacgatt tcatggagaa ggaggaagcc
     3301 gaggtcatgc cgggcctgaa gctgcccacc caggccccct ccttccttcc ctaccaggtc
     3361 aacctgctgg tggacaacag ccagacccag ggcgcaccgg tggtctggga gagcaatccc
     3421 acctacttca acctggtcgg acgccaggag taccgtcccc acctgggctc cttcctgacc
     3481 gatttcaccc tgatcaaggc cggtgccctg caccgggcca acggcggttt cctgatcctc
     3541 caggccaacg acctcttcaa caactactac tcctgggcgg ccctcaagcg ctgcctgaag
     3601 aaccgcgcca tccgcatcga ggacctggcc gagcagttcc acctgatcag caccaccgtc
     3661 agccggcccg aaccgatccc gctggagacc aaggtgatcc tgatcggcaa cccgtacatc
     3721 taccagatgc tcttcgcctg ggacgaggac ttctcgcgcc tcttcaaggt caaggccgac
     3781 ttcgaccacc ggatcgagcg cagcccgggc ggcatcaggc agtacgcggc cttcgtgagc
     3841 cgccattgcg gcgagaagaa cctgctgccc ttcgattgtt cggccctggg aaaaatcgtg
     3901 gagtacggca gccggctggt ggcggaccag aagcgcctca gcagccagtt cgcctcgatc
     3961 gtggcgatcc tggaggaggc cgattactgg gcccggcagg cctcgacgga ccgggtcggc
     4021 ggcgccgagg tccggcgggc catcgaggag cgggtgtacc ggaacaaccg gatcgaggaa
     4081 cggctgcggg agatggtcgc cgacggccag atactgattg acgaccgggg tgcggtggtc
     4141 ggccagatca acgggctggc cgttctgacc ctgggcgatt acgccttcgg ccgcccctcc
     4201 cggataacgg tgcgtacctt cgtgggcaag gagggcatcc tggacgtcga gcgggagacc
     4261 aagatgagcg gccccattca caccaagggc gtgctgatcc tgagcggctt cctcggggaa
     4321 agatacgccc acaccgaacc gctcaccctg tcggcctcga tctgcttcga gcagtcctac
     4381 gaggagatcg acggcgacag cgccagctcc accgagctgt acgccctgct ctccagcctg
     4441 gccgaactcc ccatccggca ggacatcgcg gtgaccgggt cggtcaacca gaaaggcgag
     4501 gtgcaggcca tcggcggggt caacgaaaaa atcgagggct tcttcgaggt ctgcaaactg
     4561 cgcggcctga ccggggaaca gggggtcatc atcccgaggg ccaacatcca gaacctgatg
     4621 ctcaaggaag aggtggtcga ggcggccgcc gccgggcggt tccatgtctg ggcggtcagc
     4681 tcgatcgacg aggggattga aatcctgacc ggggtggcgg ccggccagcg ccggcccgac
     4741 gggagctacc cgcccaacac cgtgaacggc cgggtcgccc gcaagttcca gaagctgaat
     4801 tcctactata ccagcagcca gcggccccgg cggcccgccc gcaaagcggc cgcccgcaag
     4861 ggcccccggg tcaaaaagta accagggaaa tccccagcgc ctcggcgcgg gcggccacct
     4921 tctccttctc caggaccagg gtgccgctcg aaatccccag cacccggccg tccgaatttt
     4981 gaagcgtccg gatggtccgc ggtcccacca ccggcaggtc aaagcgcata tcctgattgg
     5041 gacggctcac cttgaccacc accgctcccc gggtaaagcg cccggcccgc cggatagtcc
     5101 ggtcggttcc ctcaatggcc tccagggcca gcacgatccc gtccttgacc accgcggtaa
     5161 gtcccacatc gagcagcgcc agcgcctcgg ccacctggcg tcccaagatc agatccagct
     5221 cctcccgccg ggtcagggtc ggcccgtaat ggcagccggc cggcaacagc agttcgtcga
     5281 gcagaaaggt cgaaggaaga aagtgaatgc cccgatccac gaactggcgg atgagcgcct
     5341 gcagcagggt gccggtgcgc cagtctggca aaccggccag caggtcggcc agcaggggat
     5401 ccagccgttc cttgcggagc aggcggtgat ggcgaacctg gccgatcatg accgcctcct
     5461 cgacgtcatt gccgaggaaa aagttgatgc cgcgctggag attgccaatc ggcagcggca
     5521 gacagcgggt ggaaagcgag gaaacccggc ggtcggcttc ccccttgagg gccagggtat
     5581 aaacccggta atcctcacgg cgggcccgtt ccaaaaaaac cagcggcagc tttccgttgc
     5641 cggcgatcaa cccgatcttc ttcatcggta gcgtgaacgg cggctcaagg ccagtccgcg
     5701 ccgggatccg ttgataaatt ccagcaggcg gactatttcc ggcagaggcg gcaactcatc
     5761 ccgaatgtgt tggatcgcct ggctcaggtt caggccgctg cgtaccagca gccggtaggc
     5821 ccgctcgagg gttgccagcg tttcgggcga aaaatcgtgg cgtttcaggc cgacccggtt
     5881 gagacgataa atccgggccg ggtaaccgtc ggccaggcag tagggcggga tatccttgat
     5941 cacctttgaa taaccgccca ccatggcgta ggccccgatc cggcagaact ggtggaccgc
     6001 ggtcaaaccg cccaggatcg cgtggtcctc gaccgtcacg tggccggcca gggtggcgtt
     6061 attggccaga acggtgtggc tgcccacctg acagttgtgg gcaatatgac agtaggccat
     6121 gatcaggttg tcggaaccga taatcgtggc ctggtcgttt tcggtggccg ggttaatggt
     6181 cacgtactcc cggatccggt tccggtcgcc gatgcgcagg taactccgct cgccccgata
     6241 cttgagatcc tggggcggac tgccgatcac cgccccggtg tagatatagc aatcgcgtcc
     6301 gaccgtggtg tgcccggtga tgaccgcgtg cggccctacc gtggtgccgt cgccgatctc
     6361 gacctggccg tcgatgaccg cgcccgggcc gatcagcacc gatcgaccca gcttcgcttc
     6421 cggggcgact gaagccatgg ggtggatgtt catctctcct gcctgttccg ccggtttcag
     6481 acctcaatgg cccggcccgg ccgaaccctc tctttttttc tcttcgaaac gaatcgtcca
     6541 ttcgcccgga cggatgaggc ccagttcctg gcgggccagc cgttcggtgg tcaacggatc
     6601 gttttccaga agttcaatct cggccttcga ggcccggttg gccttttcca gccgcgggat
     6661 tgatttccgg tagtgctgca gcctggcgta ggaacgggtg atcttcacga agccgggcgt
     6721 gtaaacggcc aacagtccaa ccaccaggat tatccgaccc ggtcgtctca ttttcgcttt
     6781 tgaccgcgga cggccgccgg tacccgggcg taaaccgccg aaccgcccag ctcctcctca
     6841 atccgcagca gctggttgta cttggccagc cgttcggagc gggagagaga accggtcttg
     6901 atctggccgg cgccggtggc caccgccagg tcggcgatga aggtatcctc ggtttcaccg
     6961 ctgcggtgac tgatgaccga ggtgtaagaa gcccgattgg cggtctcgat cgtttccagg
     7021 gtctcggtga ccgacccgac ctggttaagc ttgatcagga tcgagttggc cgccttgcgg
     7081 gcgatcccgt cgcgcaaccg ggccgcctgg gttacgaaga ggtcgtcgcc caccagctgg
     7141 atgcggccgc cgagggccgc ggtcaaggcc acccagccgt cccagtcatc ctcggccagg
     7201 ccgtcttcaa tcgagatcac cgggtaagcc ttgacgatct tctggtagaa ttcaatcagg
     7261 tcgccaaact tgaccttccg cccttcccag gcgtaggaac ccttttcaaa gagggaactg
     7321 gcggcgacgt ccagggccag gaagacatcc cggcccggtt cgtaaccggc cttctcgatg
     7381 gcggccagga tgagcttcag tccttcctcg ttattcttga cgctgggagc gaaaccgccc
     7441 tcgtcgccaa ccgccgtgga ataacccttc tcgtgcagca ccttcttgag cgtctggaag
     7501 atctcggccg ccatccggta tccctcggaa aaggaggcgg cgccggccgg catgatcatg
     7561 aactcctgga tgtccagatt gttgtcggca tgcaggccgc cgttgatgat gttcatcatg
     7621 gggaccggca gaacgttggc cagcgtgccg cccagataac ggtaaagcgg caggccgtgg
     7681 ctgtcggcgg cggcccgggc gacggccatc gaaaccgaga ggatggcgtt ggctcccagt
     7741 ttgcccttgt tgggcgtacc gtccagttcg atcatcaacc gatccaggcc gatctggtcg
     7801 tcggcttcat aatccaggac cttggggcca ataaccaggt tgacattctc aaccgcccgg
     7861 gcgacgccct tgccgttgaa cttcttgccg ccgtcgcgca gttccaccgc ttcaaaaacc
     7921 ccggtcgagg caccggaggg aaccgccgcc cgcccgaaag aaccatcctc aaggtaaacc
     7981 tcggcctcca cggtggggtt gccgcggctg tccagtatca ttctcgcttt cacatcgact
     8041 atgcaggcca tgtgcgctcc ttgtctcgta ccggttgctt gaagtgaaca ggattaaccg
     8101 ccaggttgtt tattatacca ctttcccggg gacggcctcg acccggtccg ggcttaatcg
     8161 ggggagggac cgaccagttc ccggaggccg tcgcccagga ggttgtaagc cagaaccgtc
     8221 acgaatatca gcaggccggg cagcagcatc caggcgtggt aaagaatgac ttcgaggttg
     8281 cccatggcgc tggccaggat gttgccccag ctcgcctggg gctcctggat acccagcccg
     8341 aggatggaaa gggccgactc gcccaggatg taccccggaa tggacaggga gacggcggtg
     8401 atcgtgtagg agaaggtctg gggcagaacg tggttccaga tgatccgccg gttggagaag
     8461 cccagcgccc gcgccgccag gatgtaatcc tcctcccgga tggagaggac cataccccgc
     8521 accacccgcg ccaggccggc ccagccgatc agggccagga tcgccaccac catcaggtac
     8581 acctggtagg aggagagcgt ggtcggaaag accgctcgca gggccaggat gagatagaag
     8641 ccggggaagg acatcagcag ttcggccacc cgcatcagga ggttgtccac ccggccgccg
     8701 tagtacccgg ccagcccccc gaagagcaga cccagcagaa aggatatggt caccccgacc
     8761 aggccgatcg agagcgaaac gcggcctcca tgcaggatcc ggctgaagag gtcccggccg
     8821 aactggtcgc tgccaagcag gtagaggcgg gccggttccg gaacgcccag cagatagcgg
     8881 cggcagggaa ggaaaccgaa gattttgcgt tccggcccgg ccgggaagag gcggatgaaa
     8941 tagggcttcg aagcgtcggc ccggtaggcg atggcggcgc cttccggggg aacctggtcg
     9001 taaatgaaag ggcggaggtg ccagcggccg ccgttggcct cgcggaaacg aatcggggtg
     9061 ggcggggagt agggcttctc ccgcgtctgg aaatcgtatg ggtagggggc cagcagatcg
     9121 gccagcaggg cggccgcgta aaaaagcacc agcacccaga agctgaaaaa ggcggccggc
     9181 cgctcccgac gcagcctggt cagcagatct ttcatggccg gcgcttcccc gctttcgagc
     9241 cgcgcagctg ggccgccgat tcctcgggcc aggtgtcgtt gatgaaggtc ccggcgccgg
     9301 attcgcagcc ggccaggtac ttgagcttgt cccgggcgcg cagaggcgga tagaattcga
     9361 ggtgaaagtg gtagccgggg tgcttccggc cgtcggtcgg cgcctggtgg aagaccatca
     9421 tgtaagggaa ggaaaggtcg aagagccggt cgtaacggcc ggtgacttcc ttgagaatgg
     9481 cggccaggcc ggcccgttcc gccggcccga attcggccag cgaggcgagg tggcggcggc
     9541 tgtagacatg gacctcgtaa gaccagcggg cggcgtaggg aacgaaggcc gcgaaagtgt
     9601 cgttgaccgc caccaggcgc cgaccgtcgg ccagttccct ggccaacgtc tcgcagaaaa
     9661 ggcattggcc ggtgcgccgg tggtagcgcc gggccgaatc cagctcccgc ttgatccggg
     9721 gcggcaggta aggaaaggcg tagatctggc cgtgcgggtg gtgcagggtc acgccgacgg
     9781 gttcgccctt gttctcgaag ataaagacat acttgatccc gggcttgctt cccaggcaga
     9841 ggtaccggtc ctgccagacc tcgaccagcc gttccagctg acgccgggag agtccggcca
     9901 gggtccggtc gtggtcgctg gtgtagcaga ccacctcgca gataccgcgg gccggccgtt
     9961 cccggtagag tccgctggcc tcgcccgccg cgccggcgcc ggcctcggtc cgggtatcga
    10021 aactgggcca gcggttctca aagacggcaa cctggtagtc cggctcggtc agctcggtgg
    10081 ccggcagccc ggcccgggtg ggacagagcg ggcaccattt ccggggcggc aggaaggtcc
    10141 ggctcaggcg gtgggcggcg atgaccaccc actcttccaa caccggatcc cagcgcaggc
    10201 ggctgctcat agcgaaacga gttcccgcag cccggcggtc gatcccggcg gaccgatcac
    10261 cgccagccgg aggttggccc cggtgaagac cgccgcggcc gcccggcgga catcctcggc
    10321 ggtcacggcc gcaatgcggg cgatgatctc ctcgggcggc cggatgcggt cgcgggtggc
    10381 cagctgcccg ccgagaaaat tcatgtactc ggcggtccct tccaggtgca tctgaaactg
    10441 gccggtcacg taatcccggg cccgggtcag ttcctcctcg gggaccagtt catcccggac
    10501 ccgggccagt tcggccagga tcacccggag cgcctccttg agcgtgccgg gcgaagtgcc
    10561 cgcatcgacc gtaaagatgc cggcatcccg gaaggtgtgg tggccggccg agatctggta
    10621 ggcaaggccg cgtttttccc gcacctccac gaagaggcgg ctgctcatgt tgccgcccag
    10681 gattaccgaa agaaccgaaa ggggccagcg gtgcgggtta tcatcggaac agccccggcc
    10741 gcccaggcag aaatgggcct gttcggtctc cttctccagc agggagaaat ccggccggtc
    10801 cgcggcgggc cggaccgctt caaaaaccgg ccgcggcccc ggcttgacgg cggccaggta
    10861 attcccgacc agttcgcggg ccgcctcgac ccggaaatcg ccggccacgc aggccaccag
    10921 cgaaccggtc cggtagtggc ggtcccagta atcggagagc gtttcccggc tcaggccggc
    10981 aatcgtctcc ggggtcccgg cgatgaaccg tcccaacgga tgatcgggaa agaccagcgc
    11041 ctggaaaagc tcgtcgacgt gggcggcggg aatgtcccgg tacatcttga tctcctccag
    11101 gatgacattc cgctccttct ccacctcttc gggcggcagc cgggaaccgg tcaccatgtc
    11161 cgaaaggaca tccagggcca acggaaggtg gcgggcaaag ggcatcttga cgtaaaagca
    11221 ggtggcctct tcgccggtaa aggcgttgaa ggtgccgccg acgccctcca ccaggcgttt
    11281 caactcctcg cagctgcgcc gggcggtccc cttgaagagc aggtgctcca ggaaatggga
    11341 agccccgttc tcggccgggg cttcgtggcg gctgccgacg cgggcccaga tgcccacggt
    11401 caccg
//
LOCUS       DAOQYN010000036        11398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_6135_length_11398_cov_5.105175, whole genome shotgun sequence.
ACCESSION   DAOQYN010000036 DAOQYN010000000
VERSION     DAOQYN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11398
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6135_length_11398_cov_5.105175"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1179)
                     /gene="wecB"
                     /locus_tag="PKN80_02600"
     CDS             complement(46..1179)
                     /gene="wecB"
                     /locus_tag="PKN80_02600"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNQ34935.1"
                     /translation="MKRIILVAGARPNFMKVAPLRRELARFPGEFEPILVHTGQHYDY
                     RMSEVFFRDLELPPPDLFLGAGSGPPSEQTGRIMAGFEGALERFDPDLVVVTGDVNST
                     LAAALATAQFRCRRRSGRPRLAHLESGLRSYDRLMPEEMNRVLTDHLADLLFTTTPAD
                     GRNLRREGIPAGRVRLVGNILSDTLLSLRSRAAGPPAGLAGLKLRRRAYGLVTLHRPA
                     NVDDPKILAGLLETLAALSSDLPLLFPCHPRTRSVIGRAGLERIFRERPGFILTEPLG
                     YLDFLGLLLNSRLVLTDSGGLQAESAILKVPCLTLRDRTEWLVTLRAGGNRLAGTEPE
                     GIRRSFRQCLAGRRRPIRRPALWDGRVAGRVVRALRADLEVNS"
     gene            complement(1176..2414)
                     /locus_tag="PKN80_02605"
     CDS             complement(1176..2414)
                     /locus_tag="PKN80_02605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ34936.1"
                     /translation="MKKVLIITYHFPPARNVGSLRPAGLARHLPEFGWEPVVLTSFRP
                     GPPAAGLQVLETWPAPGERIDRSGLIRLPAAARPSYSRRLAAGLTGLVAFPDNKAGWS
                     LYAADAARPWLADASVSALISTSSPATCHLAAAALKREFPRVRWVADFRDLWTQNHYY
                     ARPRLVRRLEAKLEARTLSRADCLTTVSGPLAERLAGRYSNPVAVIENGFNPAELAPA
                     GGLLDEKFTITYTGSLYGARRDPSLLLEVLAGLAASGRIEAGRVEVNFYGRPEMSLAS
                     AAGRLGLSGLVRQHGPVPRREAVARQRSSQVLLLLNWDDPAETGVYTAKLFEYLAAGR
                     PVLALGGSGGVVRDLLERTGAGFHCADRAALEARLLDWYGQWRGSGRVGYGGRREEIL
                     KYSQREMAAKFAAVLEGKAG"
     gene            complement(2411..3451)
                     /locus_tag="PKN80_02610"
     CDS             complement(2411..3451)
                     /locus_tag="PKN80_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34937.1"
                     /translation="MNQRVLHLPVNISNQAWLLVRGLRETGWSADALFYRSNRFAYQG
                     APAGLLDRPGCGPAWFESLRAFLARAGRYDIFHFHFGHTFFSNPVDLELLRRLGKVTL
                     MHYWGSDIRFYSRAGAANPRLRERLPAEARSERKKARRARRVAGRVRACLSADLELDG
                     YLAPFFRRRYRLPQALDPGEYPCAPPPARKARPLVLHSPSNPGLKGSDFISRAVESLR
                     ASGRDFDFVQLQGVPNRQVRAALKEADIVIDQLLLGTYGIFALEAMASGKPVICFINP
                     GYRPGYPDDLPLVSAGPDDLRAVLAGLLDDGAGRFRLGEAGRAYVERHHDFRRVAERL
                     VGIYQDLCEDPA"
     gene            complement(3448..3984)
                     /locus_tag="PKN80_02615"
     CDS             complement(3448..3984)
                     /locus_tag="PKN80_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNQ34938.1"
                     /translation="MDESSSRPPYRSHGDGSFKAGDFRRLGRGVVFEAGVLVFHPEHI
                     EIGDNVYIGHGAILHGYHRNLMIVGPDSWIGPGCYLHSAGGLTIGRAVGIGPQVKILT
                     SQHPGQPADLPVMWTGLDFKPVEVGDGADLGCGSILLPGVTVGAGAIVGAGAVVTRPV
                     PAGEVWAGVPARRLKARS"
     gene            complement(4003..5109)
                     /locus_tag="PKN80_02620"
     CDS             complement(4003..5109)
                     /locus_tag="PKN80_02620"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ34939.1"
                     /translation="MSIRLSKPWLGDPSQLKRRLDEVLESGFLVQGRLVAEFEAGAAE
                     WLGVRQAVAVSSGTAALHLSLLACGIGPGDEVLVPAFTFPATANVVELAGARPVLVDV
                     DPESFNLDPDRLEAALTPRTRAIMPVHLFGNPAEMEAILALAGKHGLAVIEDAAGAFG
                     SRGRDRACGSFGAAGCFSFHPRKLITTGEGGLVVTGDPALADRLRRLRNHGLDAAGAF
                     QAAGFNYRMTELAAALGLNQLPEVDRMLAERQELAAAYRSRLAGLPGLAFQRVAGDSL
                     SAWQAFVVRIDGRENRPLVAELKRAGVEANIGTYAIHRLDYYARKYGYRPEDYPNADR
                     LYRFCLALPFYNGIPAPDLEVSARALADRLAGPV"
     gene            complement(5158..6129)
                     /locus_tag="PKN80_02625"
     CDS             complement(5158..6129)
                     /locus_tag="PKN80_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ34940.1"
                     /translation="MLTGKKFFITGGAGFIGTNLIGRLLDGNRVTVYDNLARNSLKDS
                     GLEGRPGLEVIRGDLLDFDSLLAALPKDTTHLVHLAAVAGIDTVIKNPVRTIEVNTIG
                     TYNLLRAVQAAGLTEGLERLLDFSTSEVFGINAFRVEEKTPPRMQPVGEARWTYSVSK
                     LAAEHLTHAFYKQHGLRSVILRPFNIYGPGQVGEGAIHHFVLRALRHEPLTVHGEGEQ
                     IRSWCYIDDLLDGLQLCLEKEAAVGEAFNIGNPRGTITILGLAEKVVQLAGSRSAIVH
                     APRNYVDVELRIPNIDKARQVLGFAPKVDLNEGILRTLDWYRRSLSL"
     gene            complement(6258..7511)
                     /locus_tag="PKN80_02630"
     CDS             complement(6258..7511)
                     /locus_tag="PKN80_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ34941.1"
                     /translation="MTAPRRVRHAILILTSLLYAFSYFQRVAVPGTIFNELQTEFRLS
                     ASAVAGLGTAVLYLYGGMQFVIGLLVDRLGAGRMLTAGGLLLTAGSIFFPFALSPFWL
                     FFCRLLVGLGASALYLGLVKEISEIFSESDFPFYLGIALFIGYGGGLLATLPLERLSA
                     AFGWRNALFSAGLAAGLVSILAVAVLDRTGRLVHDSSRPEMRESLVRAFRTPAIWPLA
                     FCGAINFSLYFLLQASIGKKFLEDFVRLGPARAAAFTLGMMVVMMLLAFSWGWVARRL
                     ARRRPLIVTATSLTLAAVLLLFLGVCWSRPAGWFLAAYLMLAAGSSMATVFSTAFKEM
                     APADGAASAVGIGNGTAYLGVALVATLAALIMDAFRSQAAYTERAVLYPPAAYLSVFG
                     FCLLLALVSWLVSWRVRETGPAGGG"
     gene            7668..10130
                     /locus_tag="PKN80_02635"
     CDS             7668..10130
                     /locus_tag="PKN80_02635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNQ34942.1"
                     /translation="MDYMEPSRQKLFLIDGNAVAYRAFFAIRDLSTSYGQPTNAVYGF
                     LNILQKLIRETGPDYLACVFDAPGETFRHRLFAEYKVNRPGMPENLVSQLPLIKEALA
                     AYRIPTLEEPGLEADDILRRLAIQAAGAGLEVFLVTGDKDLLQLVNDRIKVYHPQSGE
                     ILDRDRVAERFEGLTPEKIPELMALAGDSADNIPGVKGIGEKTALPLVRRFGSVENLY
                     ANLDQVASPALRRKLEAGREMAFLSRRLAELKPEPGEAAEPLDLAGLARREPDQPKLA
                     EIFQRLEFKKMLREVSADGETGPDELLFLEAAGAEDKGALDLAAETAYRPRRTTGFEA
                     LLGDPAAFRADLADPKIFKAGTDLKAKRHRLTEAGLNLEGPLFDFGLAAALCEEPAPA
                     GSPAEVCRHFGGRLKELGLARLFHEVEMPLLPVLAAMEREGIYLDAGYLQELGRHFRE
                     TLASLESEIFRQAGAAFNLNSPQQLAGVLFETLKLPPARKTRTGFSTDNAVLTVLLPL
                     HPVISLIINHRQLAKLNSTYVETLPGLVDPRDGRLHTTFNQVGAVTGRLSSERPNLQN
                     IPTESAEGVSIRRAFRRQNPGDRLYAFDYSQVELRLLAHFSGDPGLLAAFQAGRDIHQ
                     ETAAVIFNKEPGAPVTAAERRTAKVVNFGILYGMSAFGLSQQLSIAPEVAQEFIDAYF
                     KRHTGVRDWIGRTLAGARETGLVTSLLGRRRSVPEINSRRTAQRGLAERTAINTPLQG
                     TAADLVKLAMVRVSRDLAGRGLRSRMILQIHDELLFEGPESEASELADLVRRDMETVL
                     ELAVPLQVTAAAGETWADLEPI"
     gene            10368..10589
                     /locus_tag="PKN80_02640"
                     /pseudo
     CDS             10368..10589
                     /locus_tag="PKN80_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
     gene            10593..10931
                     /locus_tag="PKN80_02645"
     CDS             10593..10931
                     /locus_tag="PKN80_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34943.1"
                     /translation="MQLLANYASAPKLFWCPSASADTVAATVAALEEGTISYAYQKAA
                     SEQTPSDSPLICDQSGTKGEAFDLSPDSDTNHGVDGINILYVDGHVAWSPSGSIASNI
                     GGATMSNPGL"
BASE COUNT         2047 a         3761 c         3990 g         1600 t
ORIGIN      
        1 aaagcaagcg gacgaaactg attaattcca taaagttgtc aggtttcaag agttgacctc
       61 aagatcggcg cggagggccc ggaccacccg gccggccacg cggccgtccc agagggcggg
      121 gcggcggatc ggccgacgcc ggccggccag gcactgccgg aaggagcgcc ggatgccttc
      181 cggttcggtg ccggccaggc ggttgccgcc ggcccggagc gtgaccagcc actcggtccg
      241 gtcgcgcaag gtcaggcagg gaaccttgag gatggcggac tcggcctgga ggccgccgga
      301 atcggtcagg accagtcggc tgttaagcag caggcccagg aagtcgaggt aaccgagtgg
      361 ttcggtcaaa atgaagccgg gccgctcccg gaagatccgt tccaggccgg cgcggccgat
      421 gacggacctt gtgcgcgggt ggcaggggaa gagcagcggc aggtcggacg agagggcggc
      481 cagcgtttcc agcagcccgg ccaggatctt cgggtcgtcc acgttggccg gccggtgcag
      541 ggtgaccagg ccgtaggcgc gccggcgcag tttcagcccg gccagccccg ccggcggccc
      601 ggcggcccgg gaacgcaagc tcaaaagggt gtcggagagg atgttgccga ccagccggac
      661 acggccggcc gggatgcctt cgcggcgcag gttgcggccg tcggccggcg tggtggtaaa
      721 gaggaggtcg gccaggtggt cggtcaggac ccggttcatc tcctcgggca tcaggcggtc
      781 gtaactgcgc aggcccgatt cgaggtgggc caggcgcggc cggccggaac ggcggcggca
      841 gcggaactgg gcggtggcca gggcggcggc cagggtggag ttgacgtcgc cggtcaccac
      901 caccaggtcg gggtcgaaac gttccagcgc cccttcgaaa ccggccatga tccggccggt
      961 ctgctccgag ggcggaccgg aaccggcgcc gaggaagaga tcgggcggcg gcagttccag
     1021 gtcgcggaag aagacctcgg acatccggta gtcgtaatgc tggccggtgt ggaccaggat
     1081 cggctcgaac tccccgggaa accgggccag ttcgcgccgg agcggggcca ccttcatgaa
     1141 gttgggccgg gccccggcca ccaggattat ccgtttcatc cggcctttcc ttccaggacg
     1201 gcggcgaact tggcggccat ctcccgctgg ctgtacttga ggatctcctc gcggcggccg
     1261 ccgtaaccga cccggccgga cccgcgccac tgcccgtacc agtccagcag ccgggcctca
     1321 agcgccgcgc ggtcggcgca gtgaaaaccg gcgccggtcc gctccagcag gtcccggacc
     1381 acgccgccgg agccgcccag ggccaggacc ggccggccgg cggccaggta ctcaaagagc
     1441 ttggcggtgt aaactcccgt ttcggccggg tcgtcccagt tgagcaggag caggacctgg
     1501 ctggaacgct ggcgggccac cgcctcgcgc cggggaaccg gaccgtgctg gcgaaccagg
     1561 ccggagaggc cgaggcggcc ggcggcgctg gccagactca tttcaggccg gccgtaaaag
     1621 ttgacctcga cccgaccggc ctcgatccgg ccggaggcgg ccaggccggc caggacctcc
     1681 aggagcagag acgggtcgcg ccgggcgccg tagagactgc cggtgtaggt gatggtgaac
     1741 ttctcgtcca ggaggccgcc ggccggggcc agctcggccg ggttgaaacc gttctcgata
     1801 accgccaccg gattggaata acggccggcc aggcgttcgg ccagcggtcc ggagacggtg
     1861 gtcaggcagt cggcccggct caaggtgcgc gcttccagct tcgcctcgag gcggcggacc
     1921 agccgggggc gagcgtagta gtggttctga gtccagagat cacggaagtc ggccacccag
     1981 cggacccgcg ggaactcccg cttcagggcg gcggcggcca ggtggcaggt agccggactg
     2041 gaggtgctga tcagggctga aaccgaggcg tcggccagcc agggccgggc cgcgtccgcc
     2101 gcgtaaaggg accagccggc cttgttgtcc gggaaggcga ccaggccggt caggccggcg
     2161 gccagccggc gggaataaga cggccgggcc gcggccggga gacggatcag cccggaccgg
     2221 tcaatccgct cgcccggcgc cggccaggtt tccaagacct ggagtccggc cgccggcggc
     2281 ccgggtcgga aactggtcaa caccaccggc tcccagccga actccggcag gtggcgggcc
     2341 aggccggccg gccgcagcga accgacgttg cgcgccggcg ggaagtggta ggtgatgatc
     2401 aggacttttt tcacgcggga tcctcgcaaa ggtcctggta aatcccgacc agccgttcgg
     2461 ccacgcgccg gaagtcgtgg tgccgctcga cataggcgcg gccggcctcg ccgagccgga
     2521 aacggccggc gccgtcgtcc agcagaccgg ccaggaccgc acggaggtcg tcggggccgg
     2581 ccgaaaccag cggcaggtcg tcgggatagc cgggccggta gcccgggttg ataaagcaga
     2641 tcaccggctt gccgctggcc atcgcctcca gcgcaaagat accgtaagtg cccaggagaa
     2701 gctggtcaat gacgatgtcg gcctccttga gcgccgcccg gacctgccgg ttgggaacgc
     2761 cctgcagctg gacgaaatca aaatcccggc cagaggcccg cagcgactcg acggcccggg
     2821 agatgaaatc actccccttc aggcccgggt tggagggcga atggagcacc agcggccggg
     2881 ccttgcgtgc cggcggcggc gcgcaggggt attcgcccgg gtcgagggcc tgcggcagcc
     2941 ggtagcgccg ccggaagaag ggggccaggt agccgtccag ttccaggtcg gcggagaggc
     3001 aggcccggac gcggccggcg acccggcggg cgcggcgcgc cttcttccgc tcgctccggg
     3061 cctcggccgg cagccgctcc cggagccgcg ggttggccgc gccggcccgg ctgtaaaagc
     3121 ggatgtcgct cccccagtaa tgcatcaggg taacctttcc cagccgccgg agcagttcga
     3181 ggtcaaccgg gttggagaag aaggtatggc cgaaatggaa gtggaagatg tcataccggc
     3241 cggcccgggc caggaaggcc cggagcgatt cgaaccaggc cgggccgcac ccgggccggt
     3301 ccagcagccc ggccggggcg ccctgataag cgaaccggtt ggaccggtaa aagagggcgt
     3361 cggccgacca gcccgtttcc cgcaggccgc gcaccagcag ccaggcctgg ttggagatgt
     3421 tgaccggaag gtggagcacc cgctggttca tgaccgggcc ttcaacctcc gggccggcac
     3481 gccggcccag acctcgccgg ccggcaccgg ccgggtgacc accgcgccgg cgcccacgat
     3541 ggcgcccgcg ccgaccgtca cgccgggcag caggatcgag ccgcagccga ggtcggcgcc
     3601 gtccccgacc tccaccggct tgaagtccag cccggtccac atcaccggca ggtcggccgg
     3661 ctggcccggg tgctgggagg tcagaatctt gacctggggg ccgataccga ccgcccggcc
     3721 gatggtcagc ccgccggccg agtgcaggta gcaaccgggg ccaatccagc tgtccggacc
     3781 gacaatcatc aggttgcggt ggtacccgtg caggatggcg ccgtggccga tataaacgtt
     3841 gtcgccgatc tcgatgtgct ccgggtgaaa aacgagtacc ccggcctcga agaccacgcc
     3901 ccggcccagt cggcggaagt cgccggcctt gaaggagccg tcgccgtggc tgcggtaggg
     3961 cggccgggaa ctggattcat ccatcagacc tccttcgccc gctcaaaccg ggccggccag
     4021 ccggtcggcc agggcccggg ccgacacctc gaggtccggc gccgggatcc cgttgtaaaa
     4081 gggcagggcc aggcagaaac ggtagagccg gtcggcattc gggtaatctt cgggccggta
     4141 gccgtacttg cgggcgtagt aatccaggcg gtgaatggca taggtgccga tgttggcctc
     4201 gacgcccgcc cgcttcagct ccgcgaccag gggccggttc tcccggccgt caatccggac
     4261 cacgaaggcc tgccaggcgg aaagggaatc gccggcgacc cgctggaagg ccaggccggg
     4321 caggccggcc agccgcgacc ggtaggcggc ggccagttcc tgccgctcgg ccagcatccg
     4381 gtccacctcc ggcagctggt tgaggccgag ggcggccgcc agctcggtca tccggtagtt
     4441 gaaaccggcc gcctgaaagg cgccggccgc atcgaggccg tggttccgga ggcggcggag
     4501 ccggtcggcc agggccgggt cgccggtgac caccaggccg ccctcgccgg tggtgatgag
     4561 cttgcgcggg tggaagctga aacagccggc cgcgccgaaa ctgccgcagg cgcggtcccg
     4621 gccgcgggag ccgaaggcgc cggccgcgtc ctcgatgacc gccaggccgt gcttgccggc
     4681 cagggcgagg atggcctcca tttcggccgg gttgccgaag aggtggaccg gcatgatggc
     4741 ccgggtccgg ggggtcaggg ccgcttccag gcggtccggg tcgaggttga agctctccgg
     4801 gtcaacatcg accagcaccg gccgggcgcc ggccagttca accacgttgg ccgtggccgg
     4861 gaaggtgaag gccggcacca ggacctcgtc gcccgggccg atgccgcagg ccagcaggga
     4921 gaggtgcagg gcggcggtgc cggaactgac cgccaccgcc tgcctgacgc ccagccattc
     4981 ggcggccccg gcctcgaact cggccaccag gcgcccctgg acgagaaacc ccgactccag
     5041 cacctcgtcc agccggcgct tcagctgcga cggatcgccc agccagggct tggaaaggcg
     5101 gatggacatt ttagcgggtc ccgttcacgc gccggccgcg gccggccttt agaaaaatta
     5161 cagggagagg ctccggcgat accagtccag cgtgcggagg atgccctcgt tcaggtccac
     5221 cttcggggcg aagcccagca cctggcgggc cttgtcgatg ttggggatgc gcagttcgac
     5281 atcgacgtag ttacggggcg cgtggacgat ggccgaacgg ctcccggcca gctggaccac
     5341 cttctcggcc aggcccagga tggtgatggt accgcgcggg ttgccgatgt tgaaggcctc
     5401 gcccacggcc gcctccttct ccaggcagag ctgcaggccg tccagcaggt cgtcaatgta
     5461 acaccaggaa cggatctgct ccccctcgcc gtggacggta agcggctcgt ggcgcagggc
     5521 gcgcagcacg aagtggtgaa tggccccctc ccccacctgg ccgggcccgt aaatgttgaa
     5581 ggggcgcagg atcacgctcc gcagcccgtg ctgcttgtag aaggcgtggg tcaggtgttc
     5641 ggcggccagc ttgctgaccg agtaagtcca gcgggcctcg ccgaccggct gcatccgggg
     5701 cggggttttc tcctcgaccc ggaaggcgtt gatcccgaag acctcgctgg tcgagaagtc
     5761 gaggaggcgt tccagccctt cggtcaggcc ggccgcctgg accgcccgga gcaggttgta
     5821 ggtgccgatg gtgttgacct cgatggtccg gaccgggttc ttgatgaccg tgtcgatacc
     5881 ggccacggcg gccaggtgga ccaggtgggt ggtgtccttc ggcagggcgg caagcaggga
     5941 gtcgaagtcg aggaggtcgc cgcggataac ctccaggccg gggcgccctt cgaggccgga
     6001 gtccttcagg gagttgcggg ccaggttgtc gtaaaccgtg acccggttgc cgtccagcag
     6061 ccggccgatg aggttggtgc cgatgaagcc ggcgccgccg gtgatgaaga atttcttgcc
     6121 ggtaagcatc tttgcctcgt taaaagccgc ggcccgaaac gcgggcgcgg aatggccgcc
     6181 gggccggacc ggaaagcccc ggccttaata atgattttaa cataaaacgg agaccgccgg
     6241 aaaaggaggc gcgcccctca gccgccgccg gccgggccgg tctcccggac ccgccaagaa
     6301 accagccagg agaccagggc cagcagcagg cagaagccga agaccgacag gtaggcggcc
     6361 ggcgggtaga ggacagcccg ctcggtgtag gcggcctggg aacggaaggc gtccatgatc
     6421 agggcggcca acgtggccac cagggccacg cccaggtagg cggtgccgtt gccgatgccg
     6481 acggcgctgg cggcgccgtc ggccggagcc atctccttga aggcggtcga gaagacggtc
     6541 gccatcgaac tgccggcggc cagcatcagg taggcggcca ggaaccagcc ggccggccgg
     6601 gaccagcaga cgcccaggaa gagaagcagc acggccgcga gggtcaggct ggtggcggtg
     6661 acgatcaggg ggcggcgccg ggccaggcgc cgggccaccc aaccccagga gaaggccagc
     6721 agcatcatga ccaccatcat cccgagggtg aaggcggccg cccgggccgg gcccagccgg
     6781 acgaagtcct cgagaaactt cttgccgatg ctggcctgga gcaggaagta gagcgaaaag
     6841 ttgatcgccc cgcagaaggc cagcggccag atggccgggg tccggaaggc gcggaccagg
     6901 ctctcccgca tctcgggccg gctcgaatcg tggaccaggc ggccggttcg atcgagaacg
     6961 gcgaccgcca ggatgctgac caggccggcg gccaggccgg cggaaaacag ggcgttgcgc
     7021 cagccgaagg cggccgagag gcgctccagg ggcagggtgg ccagcaggcc gccgccgtag
     7081 ccgatgaaga gggcgatgcc aaggtagaag ggaaagtcgg actcggagaa gatctcgctg
     7141 atctccttga ccagacccag gtaaagggcg ctggcgccca ggccgaccag cagccggcag
     7201 aaaaagagcc agaaggggga gagggcgaag ggaaagaaga tggagccggc cgtcagcagg
     7261 aggccgccgg cggtcagcat ccgccccgcg cccaggcggt caaccagcag gccgatcacg
     7321 aactgcatcc cgccgtacag gtacaggacg gccgtgccca ggccggccac cgcgctggcc
     7381 gaaaggcgga actcggtctg gagctcgttg aagatggtac cgggcaccgc cacccgctgg
     7441 aagtaggaga aggcgtaaag gaggctggtc aggatcagga tggcgtgcct gacccggcgc
     7501 ggagcggtca tcttcgtctc cagtcggggc cgggcgggcg gcgcggggcg tctatccgcc
     7561 cggcccttcg aaacggttaa aggccatggc tgaaactgat agtttactac ggcggatccg
     7621 gccgcggcaa tccagccact accgggcgcg gacggtcttg tgatattatg gattatatgg
     7681 agccaagccg gcagaagctc ttcctgattg acggcaacgc ggtcgcctac cgggccttct
     7741 tcgccatccg cgacctttcc acctcttacg gccagccgac caacgccgtt tacggcttcc
     7801 tcaacatact ccagaaactg atccgggaga ccgggcccga ttacctggcc tgcgtcttcg
     7861 acgcgccggg cgagaccttc cgtcaccgac tcttcgccga gtacaaggtg aaccggcccg
     7921 gcatgccgga aaacctggtc agccagctgc cgctgatcaa ggaggcgctg gccgcctacc
     7981 gcattccgac cctggaggag cccggcctgg aggccgacga catcctgcgc cggctggcca
     8041 tccaggcggc cggggccggc ctggaggtct tcctggtcac cggcgacaag gacctgctcc
     8101 agctggtaaa cgaccggatc aaggtctacc atccccagag cggcgagatc ctggaccggg
     8161 accgggtggc cgaacgcttc gaagggctga ccccggagaa gatcccggag ctgatggccc
     8221 tggccggcga cagcgccgac aacattcccg gcgttaaggg catcggcgaa aagaccgccc
     8281 tgccgctggt gcgccggttc ggaagcgtcg agaaccttta cgccaacctg gaccaggtgg
     8341 ccagcccggc cctgcgccgg aagctggagg cggggcggga gatggccttc ctgagccgcc
     8401 ggctggccga gctgaagccg gaaccgggcg aggcggccga gccgctggac ctggccgggc
     8461 tggcccgccg ggagccggac caaccgaaac tggccgagat tttccagcgc ctggagttca
     8521 agaagatgct tcgggaggtg tcggccgacg gcgaaaccgg gccggacgag ctgctcttcc
     8581 tggaagcggc cggggcggag gataaggggg cgctggacct ggccgccgaa accgcctacc
     8641 ggccccggcg gacgaccggt tttgaagccc tgctgggtga tccggccgca ttccgggccg
     8701 acctggccga tccgaaaata ttcaaggccg gcaccgacct gaaggccaag cggcaccggc
     8761 tgacggaggc cggcctgaac ctggaggggc cgctcttcga cttcgggctg gcggccgccc
     8821 tctgcgagga gccggccccg gccggaagcc cggccgaggt ctgccgccac ttcggcggcc
     8881 gcctgaagga actggggctg gcccggctct tccacgaggt ggagatgccc ctgctgccgg
     8941 tcctggcggc catggagcgg gagggaatct acctggacgc cgggtacctg caggaactcg
     9001 gccgccactt ccgggaaacc ctggccagcc tggaaagcga gatcttccgc caggccggcg
     9061 cggccttcaa cctcaactcg ccccagcaac tggccggcgt cctctttgaa accctgaaac
     9121 tgccgccggc ccgcaagacc cgaaccggct tctcaaccga caacgcggtc ctgaccgtcc
     9181 tgcttccgct ccacccggtc atctccctga tcatcaacca ccggcagctg gccaagttga
     9241 actcaaccta cgtggagaca ctgcccgggc tggtcgaccc gcgggacggc cgcctccata
     9301 ccaccttcaa ccaggtgggc gcggtcaccg gccgcctctc gagcgagcgg cccaacctcc
     9361 agaacatccc gaccgagtcg gccgagggcg tctcgatccg gcgcgccttc cgccgccaga
     9421 atccgggcga ccggctctac gccttcgact actcccaggt ggaactccgc ctgctggccc
     9481 atttttcggg cgacccgggg ctgctggccg ccttccaggc cggccgggac atccaccagg
     9541 aaacggccgc ggtcatcttc aacaaggagc cgggcgcgcc ggtgaccgcc gccgagcgcc
     9601 ggacggccaa ggtggttaac ttcggcatcc tttacggcat gagtgccttc ggcctcagcc
     9661 agcagctgtc catcgcgccg gaggtggccc aggagttcat cgatgcctac ttcaagcgac
     9721 acaccggcgt ccgcgactgg atcggccgca ccctggccgg cgcccgcgaa accggcctgg
     9781 tcacctcgct gctggggcgg cgccgctcgg tcccggagat caacagccgg cgcaccgccc
     9841 agcgcggcct ggccgaacgg accgccatca acacgccgct ccagggaacg gcggccgacc
     9901 tggtcaagct ggccatggtc cgcgtcagcc gcgacctggc cggccgggga ctgcgaagcc
     9961 ggatgatcct ccagatccac gacgagctcc tctttgaggg accggaatcc gaggcctcgg
    10021 agctggccga cctggtgcgc cgggacatgg aaaccgtcct ggagctggcc gtcccgctcc
    10081 aggtgacggc cgccgccggc gagacctggg ccgacctgga gccgatctga agacgggacg
    10141 gccattaaac gaaaaccgga cggattccaa cttaattgag agtggaaaag aaacgaaatc
    10201 acggtgccgg acgccacttg acaggggcag ttgaacccgg tataatacgg ggtgtaaatt
    10261 aaatatagtc ccgcaggtac ttgacaaggc gtttcagttg ggctataatg ggcccagaat
    10321 gttgaagtag caacaaaaat ctcaaaacga aagggggtga ataaacgatg aaatcgagaa
    10381 agggtttcac cctgatcgaa ctcttggtgg taattgccat tatcgccatt ctggcggcaa
    10441 tgcttctgcc ggcacttgcc agggcgagag agcaggctcg gcgttccaac tgtctcagca
    10501 acctgaaaca gcttggtctg gctctgcaca tgtactcgca ggactacgcc gagaagttcc
    10561 cgaaggccac ggttgacacg gtggtcaacg acatgcagct actggcgaac tacgcctcgg
    10621 cacccaagct tttctggtgc ccgagcgcca gcgccgatac ggtcgctgcc acggtagccg
    10681 cccttgagga aggaaccatc tcctacgcct accagaaggc cgccagcgaa cagaccccgt
    10741 ccgactcacc gctcatttgt gaccagagcg gcaccaaggg cgaggcgttc gacctgtcgc
    10801 cggattccga caccaaccac ggagttgacg gcatcaacat cctgtatgtt gatggtcacg
    10861 tcgcctggtc cccgtcgggt tccatcgcca gcaacatcgg cggcgctacg atgtccaacc
    10921 cgggtcttta atacgcccgg cacaacaagc gaagtagaga ggcaggggct tcaaaacccc
    10981 tgcctctttt ttttgtgttt ttgcgcgggg cagtaaacac gacgcccctt cccgccccct
    11041 gaagccttcg acaggctcag gcttgataaa accaattcga caagctcagc cctcttcttc
    11101 gaagagcctc gacatcctcg gtttacactt cagggttttg gatcggtttt tcagacgaat
    11161 tgtaaaacgc ccgtcatgcc cacggatagt taggtgagta tccaacaaac atgtcatgcc
    11221 cgtatgttgt agacgggcat ccaatcctta agaggtttta actgataaat acatggattc
    11281 ccgcctacta tcctcctacg ctgaaaaatg agcttcggcg gacaagtccg cgggaatgac
    11341 aggcaaaaaa cgtggcttgt aatcgccacc tacgctatcc ttgtagttgc ctttcgtc
//
LOCUS       DAOQYN010000037        11306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_6193_length_11306_cov_5.365123, whole genome shotgun sequence.
ACCESSION   DAOQYN010000037 DAOQYN010000000
VERSION     DAOQYN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11306
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6193_length_11306_cov_5.365123"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PKN80_02650"
     CDS             complement(<1..536)
                     /locus_tag="PKN80_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNQ34944.1"
                     /translation="MADLFLGFDSGTTNTKAFLYDDRLVLKAAAARPTGLKFPRPGWA
                     EQPADDWFLALARSLRGLKTRLGTIGLAGSSQGGTFVPLDRNGRPLRPAVTWLDNRAP
                     DIAAELNRRFGAGRFYRKTGWRLYGGSPLTLCRWLQQEEPEVWRHTRRLAFVADYLNY
                     RLTGEFFLDSTSAAMTNL"
     gene            complement(540..1943)
                     /locus_tag="PKN80_02655"
     CDS             complement(540..1943)
                     /locus_tag="PKN80_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34945.1"
                     /translation="MKPRIGLIGLTAELYRRKVPELVAGLETFSRELAGRLERELELT
                     HVPVVCTRPEMEAAVEKLAAAGVDGVILVFLSYSPSLIIAPALKKLGGRPILVWNTQK
                     LETVGTEFSGADMFKNHGMHGVQDLASVMRREGLAFHLITGHYRDAAARAGVNDWCRA
                     AAAAGRLGRARLGRLGGRFEDMGDFAWDDRRLREQLGPQAVDLPVSRLSECSRAVPEK
                     EIQGLVAAENRRFRVDPKLDPATRRQAARLELGLRRLVAEERLNGLGINFMAFEGAEG
                     AEAIPFAAISKLMAEGLGYGGEGDILCAAAVLILHGLAGAGNFVEMFTTDYRNDRIFM
                     SHMGESNPNLARDPKSVRLARKEMSLIKPGLATTSLLFQLKPGPVTLLNLAPGRDGLN
                     FIMARGRVLAKPWFPGLDVPNFMLKIPGRVENFLTGYSRLGGTHHLGLAYGDQRPALR
                     LLAEQLGRPAFEVGEEG"
     gene            complement(1982..2746)
                     /locus_tag="PKN80_02660"
     CDS             complement(1982..2746)
                     /locus_tag="PKN80_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34946.1"
                     /translation="MTGKEIRTARLFSNGKPIVVAIDHGQYFGPIPGLVDLAETARQV
                     ASADAILMSPFVLSRVKPVFYRKEGPSLILRVNWTTALCMPWQYREAHTHPTLEAEEA
                     LALGADLVLANLTLQSGCEKTDAENVRVFTKIARQKERAGLPLVGEVIPLIPLTETER
                     LHRHIKEAIRIAWELGADLIKTYYTGPRFNEVVAGVPIPVFALGGAKFKNEADALALA
                     RNAFRAGAAGLVFGRNVIQARKPDRFIQQLKAAAAV"
     gene            complement(2743..3519)
                     /locus_tag="PKN80_02665"
     CDS             complement(2743..3519)
                     /locus_tag="PKN80_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNQ34947.1"
                     /translation="MGIGQLRRTDLIRRALEEENLVSVAALVRRTGASTATIRRDLER
                     LEKTGFLVRTYGGAVRQDIPDEFLFPRKAERNRAAKAAIARRALALLKEGDTIFLDSG
                     TTAREMARLIGRTDRTFTVVTNSLPAAGELQRNQSLKIFLLGGFLRRELLDFFSPAVL
                     AELEKFHLNKAFLGIDGLTAADGLTTTDVDSARIEETAMRISQVVVVLADRSKIGRTT
                     LIPYGTLKKIDCLITDRGADPRELSRLRRAGLKIILTEGK"
     gene            complement(3621..4610)
                     /locus_tag="PKN80_02670"
     CDS             complement(3621..4610)
                     /locus_tag="PKN80_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ34948.1"
                     /translation="MTNRERFTRTFEFQPVDRLPVVEWAPWWDQTIKRWRTEGLPAEL
                     TAVVAIQDWFGLDRFQIRKLSAMRPEYGQLEKADRLIADLEGYRRLKDFLYPGTGQAT
                     AWLAEAADAQRNRDIVTGLILEGPFWFPRILFGIEAHLYAFYDHPEAMRLIIEDLADY
                     HLRILEAARQTIRPELVLFSEDMSYNHGPMLSRKTFDEFISPYYRRIVPRLKEFGWPA
                     VIDSDGQIGPLLEWFAPTGVDAFLPLEHQAGVDIVELRRRHPRHRFIGAFDKMVLNRG
                     EAAVEAEFERLRPVMAGGGYVPGVDHQTPPGVSLAEYRDYLVRLAGWCRRAAG"
     gene            complement(4618..6048)
                     /locus_tag="PKN80_02675"
     CDS             complement(4618..6048)
                     /locus_tag="PKN80_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNQ34949.1"
                     /translation="MIIYKNAPEEIAGYLEDSSNFRRGAADGLYLPENESEVVRVLTE
                     CSEAGQPLTISAGGTGTVAGRIPVGGRIISVERLDRILTIDPAAQRASLQAGVVVEKF
                     LAAIDRVGRFYPPFPTERTAMISGNVSTNASGEYSYRFGPTRQYVNRLRLVLSDGEVI
                     DLRRGEVRADANGRLEIGNKVIRIPDYRTPEIKCSAGYFSRPGLDAIDLFIGSEGTLA
                     VFTEVEVKLIPALPPRMIMLLFLSDEARMLPLLEEIRGQRKLETLSLEYFDRHSLDFL
                     VQDFPQIPAEARFAFYVEGLAGELETWLEILEKYPLVDTWVGADPAAYEKLIGFRHRL
                     PENINNYFKTIKSLKVSLDFAVPGRHFHELYRFYQEMRSANRHLETVLFGHIGENHLH
                     FNFFPKNEAEREEAYGLYREAALKVIALGGTIAAEHGIGKIKYQWLELMYGREGIAQM
                     VAVKKVLDPAGILGLDTIFPRELVKG"
     gene            6137..7120
                     /locus_tag="PKN80_02680"
     CDS             6137..7120
                     /locus_tag="PKN80_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34950.1"
                     /translation="MIKVALVGAGFMGGMHSACYQAVEGAELAWVVDTDADRAAKLAG
                     RHEARPTVDLDEALADPAVSLVDVCLPTDQHPENVIRACRAGKNVICEKPIALTLAEA
                     DRMIAAARENRVRFMVAMVIRFWPAYQRLTEIYRSGELGKLQALTLQRVSSLPTWTWK
                     DWILDRKRSGGAVLDMHIHDTDFAYHLLGLPRQIESRLAPTNHGFIYSVFSYPGTTVM
                     IEGGWHEARSCPFFSAYEAVFEKGVVSYDTRLVPDFTIYREDRKENPVLPSRKGGGDS
                     GGNISDLGGYYNEIAYLIDCLERGREPELASAQSARESLALLLQELEQAGL"
     gene            complement(7238..8755)
                     /locus_tag="PKN80_02685"
     CDS             complement(7238..8755)
                     /locus_tag="PKN80_02685"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HNQ34951.1"
                     /translation="MAELEFQKAPGRTTELQAEFTNLEVYPDSTVATAGRTYALARSE
                     KGKQLLVKGDETDLRRAGFEGTMIAGRILVGELTPANAAALRKALDYLRPVRSEGRTS
                     IGLGDRLGLATPGHIRAIEGTNLFPYLAQQSIREMARTGRHPQQVMDEALWGIFQTGY
                     TDGFGSDADHLKTPADLADCLRAGFTLFTIDPGDQVDNQADTDAPETLLSKCRGLGAE
                     APDLNLDRLRKSYLSGKVKLPGGSSLEFSETELLRAAVKYGRAIKHTVMMYQTLKSAA
                     AGRTFELEMSVDETLTPTTVGEHYFVASELARAGVTWVSLAPRFVGRFEKGVDYIGNL
                     DEFRKELIRHRAVARQLGNYKISIHSGSDKFSLYPTIAEVTEGRVHLKTAGTSYLEAV
                     RTIADTNPELYRAIYRFARERYETDRASYHVSAKIDRVPPENALSNGDLEASLDDFHT
                     RQMLHVTFGSVLTARTEDGSYRFRTEFYRTLEAEELEHYRCLAKHLGRHARPFAR"
     gene            complement(8766..10010)
                     /locus_tag="PKN80_02690"
     CDS             complement(8766..10010)
                     /locus_tag="PKN80_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34952.1"
                     /translation="MKQRKRYALVGASGRGLGMYAYPLKEEYADVAEVVGIFDPNPVR
                     SAYVKEFCRLDCRTYTDYDRMIRETRPETVIVTTVDRYHHEYIIRALEAGLDAITEKP
                     MTVDLEKCRQILETEARTGRKVIVTFNYRFTPYITRIRELLRDGLVGTILNVDFEWLL
                     DTRHGADYFRRWHRRKENSGGLLVHKATHHFDAINWWVGEEPEVVNAFGSLRFYGPNR
                     KKRGQRCLTCRHRSSCEFYFDLTANERMVKMYLEAEKHDGYHRDGCVFSEEIDIEDSM
                     SVNVRYSGGALLTYSLVAHSPYEGFRASVSGTDGRIEVAEYQSGDSTGGTHQTVTFFN
                     RRNERITYQVPKATGGHGGGDTRLRRMLFRGDLPDPLNHMAGSYEGAISMLIGAAANR
                     SIARGRAIRIQDHVPLADYRKR"
     gene            complement(10054..11091)
                     /locus_tag="PKN80_02695"
     CDS             complement(10054..11091)
                     /locus_tag="PKN80_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNQ34953.1"
                     /translation="MSARRNFSMQSYWNRKFEDWKPLLAFRGRSRNDWRTWRKKALPK
                     LLELLGDFPKPVPLQAEVEYSVTEGDLVRERVVLNSEAFMSVPCLLLKPRRMAADRKQ
                     AAILCSHGHGLHGKDAVAGFRSSAAVEQNIAAHNYNYAEQMARAGFITLAPDLRVFGE
                     RRDGADPFPGRDPCNINFLKGAMLGVYTLTLNIWDMKCCVDYLQTRPEVDPDRIGMMG
                     LSQGGTMTTFTTAVEPRIRAADIIGYVNSWRGFAIERANWCGSQMVPNLYRYFDTDDI
                     AGLIAPRPLLLEMGIYDTCFFIQDQLEGYRGVERIYRAAEAAADLWTDIHPGPHAFSG
                     RRAFDFFKKYL"
     gene            complement(11124..>11306)
                     /locus_tag="PKN80_02700"
     CDS             complement(11124..>11306)
                     /locus_tag="PKN80_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34954.1"
                     /translation="VLMCPQGGDRRDLAAYIKDLLPRVPHGNLFALGMSDNVPSDADF
                     DRVKIVADLVNNYRPA"
BASE COUNT         1841 a         3454 c         3886 g         2125 t
ORIGIN      
        1 aagagattgg tcatggccgc cgaggtcgaa tcgaggaaga attcgccggt gagtcgataa
       61 ttcaggtaat cggccacgaa ggccagccgc cgggtgtgcc gccagacctc cggttcctcc
      121 tgctgcagcc agcggcagag ggtgagcggc gagccgccgt agagtcgcca gccggttttc
      181 cggtagaaac ggccggcccc gaagcggcgg ttgagctccg cggcgatgtc gggcgcgcgg
      241 ttgtcgagcc aggtgacggc cggtcgcagg gggcgcccgt tcctgtcgag cggaacgaag
      301 gtgccgccct gggaactgcc ggccaggccg atggttccca ggcgcgtttt caggccgcgg
      361 agactccggg ccagggccag gaaccagtca tcggccggct gttcggccca gccgggccgg
      421 ggaaatttca ggccggtcgg tcgggcggcc gcggccttca agaccagccg gtcgtcgtaa
      481 aggaaggcct tggtgttggt ggtgccgctg tcgaagccca ggaagagatc ggccatcttt
      541 tatccttctt ctccgacttc gaaggccggc cggccaagtt gttccgccag cagccgcagg
      601 gccggccgct ggtctccgta agccaggccc aggtgatggg tgccccccag ccggctgtag
      661 ccggtgagaa aattctccac ccggccgggg attttcagca tgaagttggg aacgtccagc
      721 ccggggaacc agggtttggc caggacccgg ccgcgggcca tgatgaagtt caatccgtcg
      781 cggccgggcg ccaggttgag aagcgtgacc ggccccggct tgagctggaa gagcaggctg
      841 gtggtggcca ggccgggctt gatcagcgac atctccttgc gggccagccg gaccgatttc
      901 gggtcgcggg ccaggttggg attgctttcg cccatgtggg acatgaagat ccggtcgttc
      961 cggtagtcgg tggtgaacat ctcgacgaag ttgccggcgc cggccaggcc gtgcagaatc
     1021 agcaccgccg ccgcgcagag gatatcgccc tcgccgccgt agcccagtcc ttcggccatc
     1081 agcttggaga tggcggcgaa gggaatcgcc tcggccccct ccgctccctc gaaggccatg
     1141 aaattgatgc ccagtccgtt caggcgctct tcggccacca ggcgccgcag gcccagttcc
     1201 aggcgggccg cctgccggcg ggtggccggg tcaagcttcg ggtccacccg gaaacgccgg
     1261 ttctccgcgg ccaccagccc ctggatttcc ttctccggaa ccgcgcgtga gcattcggac
     1321 agacggctta ccggcaggtc gaccgcctgc ggtccgagtt gttcgcgcag ccggcggtcg
     1381 tcccaggcga aatcgcccat gtcttcaaaa cggccgccca gccggcccag gcgcgcgcgg
     1441 cccagccggc cggccgcggc ggccgcccgg caccagtcgt tcaccccggc ccgggccgcg
     1501 gcgtcccggt agtggccggt gatcaggtgg aaggccaggc cttcccgccg catgacgctg
     1561 gccagatcct ggacgccgtg catgccatgg tttttgaaca tgtccgcgcc cgaaaattcc
     1621 gttccgaccg tctccagttt ctgggtgttc cagaccagga tcggccggcc tccgagtttt
     1681 ttgagcgccg gcgcgatgat caggctcggg ctgtaggaga ggaagaccag gattaccccg
     1741 tcgacgccgg cggcggccag tttttcgacg gccgcctcca tctccggccg ggtgcagacg
     1801 accggcacgt gggtcagctc gagttcccgt tcgaggcggc cggccagttc cctggagaag
     1861 gtctccagtc cggctaccag ctccgggacc ttccggcggt aaagttcggc ggtcaggccg
     1921 atcagcccga tccggggttt catgtcatct ccttttttcg aggtctgatg agagccgttt
     1981 ttcagacggc cgcggccgcc ttcagctgtt ggatgaaccg gtccggtttc cgggcctgga
     2041 tgacgttgcg gccgaagacc agcccggcgg cgccggcccg gaaggcattg cgggccaggg
     2101 ccagggcgtc ggcctcgttt ttgaacttgg ccccgccgag ggcgaagacc gggatcggca
     2161 ccccggcgac cacctcgttg aagcgcgggc cggtgtagta ggttttgatc aggtcggcgc
     2221 ccagctccca ggcgatccgg atcgcctcct tgatgtgccg gtgcaggcgt tcggtctcgg
     2281 tgagggggat gagcggaatg acctccccga cgagcggcag gccggcccgt tccttctggc
     2341 gggcgatctt ggtgaagacc ctgacgttct cggcgtcggt cttttcgcag ccgctctgga
     2401 gggtcaggtt ggccaggacc aggtcggcgc ccagcgccag ggcctcttcg gcctccaggg
     2461 tcgggtgggt gtgggcctcc cggtattgcc agggcatgca gagggcggtc gtccagttga
     2521 cccggaggat cagcgagggg ccctccttcc ggtagaagac cggcttgacg cgcgaaagca
     2581 cgaagggact catcaggatg gcgtcggccg aggcgacctg gcgggcggtt tcagccaggt
     2641 cgaccaggcc gggaatggga ccgaagtact ggccatggtc gatggccacc acgatcggct
     2701 tgccgttcga gaaaaggcgg gcggttcgga tttcctttcc ggtcattttc cctccgtgag
     2761 gatgattttc aacccggccc gccgcaggcg gctcaattcc cgcgggtcgg cgccccggtc
     2821 ggtgatcagg cagtcgatct tcttaagcgt gccgtaaggg atgagcgttg tgcggccgat
     2881 cttgctgcgg tcggccagca ccaccaccac ttggctgatc cgcatggcgg tctcctcgat
     2941 ccgggccgag tcgacgtcgg tggtggtcag gccgtcggcg gcggtcaggc cgtcgatgcc
     3001 caggaaggcc ttgttgagat gaaacttttc cagctcggcc aggacggccg ggctgaagaa
     3061 atcgagcagc tcccggcgca ggaagccgcc gagcaggaag atcttcaggc tttgattgcg
     3121 ctggagttcg ccggcggccg gcagcgagtt ggtgaccacg gtgaaggtcc ggtcggtccg
     3181 gccgatgagc cgggccattt cccgggcggt ggtgccggag tccaggaaga tggtgtcccc
     3241 ctctttcagc agggccaggg cgcgccgggc gatggctgcc ttggccgccc ggttccgctc
     3301 ggccttgcgc gggaagagaa attcgtccgg aatgtcctga cggaccgccc cgccgtaggt
     3361 gcggaccagg aaaccggtct tctccaggcg ttccagatcg cggcggatcg tggccgtgga
     3421 agcgccggtc cggcggacca gcgccgccac cgaaaccagg ttttcctctt ccagcgcccg
     3481 gcggatcagg tcggtccggc gcagttggcc gatgcccata tggattatat tattatcttt
     3541 ttgtttgttt tatgtcaagt tttgattgat tttgattgtt tggcaaaccg gcccgtttcg
     3601 gccgggagtg agccttgttt tcagccggcg gcccggcggc accagccggc caggcggacg
     3661 agatagtcgc ggtattcggc cagggagacg cccgggggtg tctggtggtc cacgccggga
     3721 acgtagccgc ccccggccat gaccggtcgg aggcgttcga actcggcctc cacggccgcc
     3781 tcaccccggt tgaggaccat cttgtcgaag gcgccgatga atcggtggcg tggatggcgc
     3841 cggcgcagct cgacgatgtc cacgccggcc tggtgctcca ggggcaggaa ggcatccacg
     3901 ccggtcggcg cgaaccactc cagcaggggg ccgatctggc cgtcggagtc gattaccgcc
     3961 ggccagccga actccttcag gcgggggacg atgcgccggt agtaggggga aatgaactcg
     4021 tcgaaggtct tgcgggagag catcgggccg tggttgtacg acatgtcctc gctgaagagg
     4081 accagttccg ggcggattgt ttgccgggcc gcctcgagga tccggaggtg gtaatcggcg
     4141 aggtcctcga ttatcaggcg catcgcctcc gggtgatcgt agaaggcgta gaggtgggcc
     4201 tcgatgccga agagtatgcg cggaaaccag aaggggccct cgaggatcag gccggtgacg
     4261 atgtcgcggt ttcgctgggc gtcggccgct tcggccagcc aggcggtggc ctggccggtg
     4321 ccggggtaga ggaagtcctt gaggcgccgg tagccttcca ggtcggcgat cagccggtcc
     4381 gctttttcga gctggccgta ctcggggcgc atggcgctga gtttccttat ctggaaacgg
     4441 tccagtccga accagtcctg gatcgcgacc acggcggtaa gctcggccgg caacccctcg
     4501 gtccgccagc gcttgatggt ctggtcccac cagggggccc actcgaccac cggcagccgg
     4561 tcgaccggct ggaattcgaa cgtccgggta aagcgttcgc ggttggtcat cggccgatca
     4621 tcctttcacc agttcgcggg ggaagatggt gtccaacccc aggattccgg ccgggtcgag
     4681 gaccttcttg accgccacca tctgggcgat gccctcccgg ccgtacatga gttccagcca
     4741 ctggtacttg attttgccga tcccgtgctc ggcggcgatg gtgcctccca gcgcgatgac
     4801 cttgagggcc gcctcccggt agagaccgta ggcctcctcc cgttcggcct cgttcttcgg
     4861 gaagaagttg aagtggaggt gattctcgcc gatgtggccg aagaggaccg tctccagatg
     4921 gcggttggcg gaacgcatct cctggtaaaa gcggtagagc tcgtggaagt ggcggcccgg
     4981 cacggcgaaa tcaagcgaga ccttgagcga cttgatggtt ttgaagtagt tgttgatgtt
     5041 ctccgggagc cggtgacgga agccgatcag cttttcgtag gcggccgggt cggccccgac
     5101 ccaggtatcg accagcgggt acttctccag gatctccagc caggtttcca gttcgccggc
     5161 cagaccctcg acgtagaagg cgaaccgggc ttcggccggg atctggggga aatcctggac
     5221 caggaagtcc aggctgtgcc ggtcgaaata ctccagggag agggtttcga gtttccgctg
     5281 accgcggatc tcctccagca gcggcagcat tcgtgcctcg tcggagagga agagaagcat
     5341 gatcatgcgc ggcggcaggg ccgggatcag tttcacctcg acctcggtga agacggccag
     5401 cgtgccttcc gagccgatga agaggtcgat ggcgtccagg cccgggcgcg agaagtaacc
     5461 ggccgagcac ttgatttccg gcgtgcggta gtcggggatc cggatcacct tgttcccgat
     5521 ttccagacgg ccgttggcat cggccctgac ctcgccccgg cgcaggtcga tcacctcgcc
     5581 gtcggagagg accaggcgca ggcggtttac gtactggcgg gtcggtccga agcggtaact
     5641 gtactcgccg gaggcgttgg tggagacatt gccggagatc atggccgtgc gttcggtggg
     5701 gaagggcgga tagaagcggc cgacccggtc gatcgccgcc aggaacttct cgaccaccac
     5761 gccggcctgg agagaggccc gctgcgcggc tggatcgatc gtcaggatgc ggtcgagccg
     5821 ttccactgaa atgatgcggc cgccgaccgg gatgcggccg gccacggtcc cggtgccgcc
     5881 ggccgagatg gtgagcggct gtccggcctc actgcattcc gtcagcaccc ggacaacttc
     5941 ggattcgttt tcaggcagat agagaccgtc ggccgcgccc cggcggaagt tggaactgtc
     6001 ctccaggtag ccggcgattt cttccggagc gttcttatag attatcattt ttatatttta
     6061 acccaaacga ttgacggaaa ccaaaatttc tgttatcttt tttgcgttgg accaacgcga
     6121 aaaaggagat ggagtgatga tcaaggtggc attggttggc gctggtttca tgggtggcat
     6181 gcattcggcc tgttaccagg ccgtcgaggg ggccgaactg gcctgggtgg tggacaccga
     6241 cgcggaccgg gccgccaagc tggccggccg gcacgaggcc cgtccgaccg ttgacctgga
     6301 cgaggcgctg gccgatccgg ccgtttcgct ggtggatgtc tgcctgccga ccgaccagca
     6361 cccggagaac gtcatccggg cctgccgggc cggcaagaac gtcatctgcg agaaaccgat
     6421 tgcccttacc ctggccgagg ccgaccgcat gatcgccgcc gcccgggaga accgggtcag
     6481 gttcatggtg gccatggtga taagattctg gccggcttac cagcggttga cggaaattta
     6541 ccggagcgga gaactcggga aactgcaggc cctgaccctg cagcgggtct cttcgctgcc
     6601 cacctggacc tggaaggatt ggatcctcga ccggaagcgc agcggcggcg cggtgctgga
     6661 catgcacatc cacgataccg atttcgccta ccacctgctc ggtcttcccc ggcagatcga
     6721 gagccggctg gcgccgacca accacggttt catctactcg gttttcagtt acccggggac
     6781 cacggtgatg atcgagggcg gctggcacga ggcccggagc tgtcctttct tctcggccta
     6841 cgaggcggtc ttcgagaaag gcgtggtcag ttacgacacc cggctggtcc cggacttcac
     6901 catctaccgg gaggaccgga aagaaaaccc ggttctgccc agccgcaagg ggggcggcga
     6961 cagcggcgga aatatctccg acctgggcgg ttactacaac gagatcgcct acctgatcga
     7021 ctgcctcgaa cggggccggg aacccgaact ggccagcgct cagagcgccc gcgaatcgct
     7081 ggctctgctg ctccaggagc tggagcaggc cggtctctga cgccgccaac aaagctggcc
     7141 gggccgaagg cggaccgtca agtttcaggt ctcgaattcg gaccggaatc agctctggga
     7201 attaccttga gtaatccgga ccgaaggcgg accgggatca gcgggcaaag ggccgggcgt
     7261 gccttcccag gtgcttggcc aggcaccggt agtgctccag ttcctcggct tccagggttc
     7321 ggtaaaattc cgtccggaac cggtaggaac cgtcttccgt ccgggcggtg aggaccgagc
     7381 cgaaggttac gtgcagcatc tgccgggtat gaaaatcatc gagggaagct tccaggtctc
     7441 cgtttgagag tgcgttctcc ggcggcaccc ggtcgatttt ggccgagacg tggtaggagg
     7501 cccggtcggt ctcgtaacgt tcgcgggcga aacggtagat ggcccgataa agttccgggt
     7561 tggtgtcggc aatggtccgg accgcctcca gatagctggt gccggccgtc ttcaggtgaa
     7621 cccttccttc ggttacctcg gcgatcgtcg ggtagaggct gaatttatcc gagccggagt
     7681 ggatggaaat cttgtaattc ccgagctgcc gggccaccgc ccggtggcgg atcagctcct
     7741 tccggaattc atccaggtta ccgatgtagt ccaccccttt ttcgaagcgg ccgacgaagc
     7801 gcggcgccag gctgacccag gtcaccccgg cccgggccag ttccgaggcc acgaaataat
     7861 gttcgccgac ggtggtgggc gtcagtgttt cgtcgaccga catctcgagt tcgaaggtgc
     7921 gtccggccgc ggccgatttc agggtctggt acatcatcac cgtgtgtttg atggcccggc
     7981 cgtatttgac ggcggcccgg agcagttcgg tctccgaaaa ctccaggctg cttccgccgg
     8041 gcagttttac cttgccggac aggtatgatt tgcgcaggcg gtccaggttg aggtcgggcg
     8101 cctcggcccc caggccgcgg cacttggaaa gcagcgtttc gggggcgtcg gtgtcggcct
     8161 ggttgtcaac ctggtcgccg gggtcgatcg taaaaagggt gaagccggcc cgcaggcaat
     8221 cggccagatc ggccggggtc ttgagatggt cggcgtccga accgaagccg tcggtgtagc
     8281 cggtctggaa gatgccccag agggcctcgt ccatgacctg ctgcgggtgg cggccggtgc
     8341 gagccatctc ccggatggac tgctgggcca ggtaaggaaa gaggttggtt ccttcgatgg
     8401 cccggatgtg gccgggcgtg gccaggccga gccggtcgcc caggccgatc gaggtccgcc
     8461 cttccgaacg gaccggccgc aggtaatcga gggccttccg aagcgcggcg gcgttggccg
     8521 gcgtgagttc tcccaccagt atccggccgg cgatcatcgt tccttcaaag ccggcccggc
     8581 gcaggtcagt ctcatcccct ttgaccagca gctgtttccc cttttcggaa cgggccagcg
     8641 cgtaggttcg cccggccgtg gccaccgtgg aatccgggta gacctccagg ttcgtgaact
     8701 ccgcctggag ttcggtcgtt cggccgggtg ccttctgaaa ttcaagttcg gccatgttgc
     8761 ctcccttagc gtttgcggta gtcggccagc ggaacgtgat cctggatgcg gatcgcccgg
     8821 cccctggcga tggaacggtt ggccgccgcc ccgatcagca tcgagatggc gccctcgtat
     8881 gaaccggcca tgtggttcag gggatcgggc aggtcgcccc ggaagagcat ccgccgcagc
     8941 cgggtatcgc cgccaccatg gccgccggtg gccttgggca cctggtaggt gatccgctcg
     9001 ttccgccggt tgaagaaggt gaccgtctgg tgcgtgccgc cggtgctgtc gccgctctgg
     9061 tactcggcga cctcgatccg gccgtcggtg ccgctgacgc tggcccggaa tccctcgtag
     9121 ggggagtggg cgaccagcga gtaagtcagc agggcgccgc cggaataccg gacgttcacg
     9181 ctcatgctgt cctcgatgtc gatttcttcg gagaagacgc agccgtcgcg gtggtacccg
     9241 tcatgtttct ccgcttccag gtacatcttg accattcttt cgttggcggt gagatcgaag
     9301 taaaactcgc agctggaacg gtggcggcag gtcaggcagc gctggccgcg ttttttccgg
     9361 ttcggcccgt aaaagcgcag gctgccgaag gcgttgacca cttccggttc ttcgcccacc
     9421 caccagttaa tggcgtcaaa atggtgggtg gccttgtgga ccagcagccc gccggagttt
     9481 tccttgcgcc ggtgccagcg gcggaaataa tcggcgccgt ggcgggtgtc cagcagccac
     9541 tcgaaatcga cgttgaggat ggtgccgacc aggccgtccc ggagcagttc ccggatcctg
     9601 gttatgtagg gggtgaaccg gtaattgaag gtgacaatga ccttgcggcc ggtgcgggcc
     9661 tcggtctcca ggatctggcg gcacttctcc aggtcaacgg tcatcggctt ctcggtgatc
     9721 gcgtccaggc cggcctccag ggcccggatg atgtactcgt ggtggtaccg gtcgaccgtg
     9781 gtgacgatga ccgtttccgg ccgggtctcc cggatcatcc ggtcgtaatc ggtgtaggtc
     9841 cggcagtcca gccggcagaa ttccttgacg taggcggacc ggaccgggtt ggggtcgaag
     9901 atgccgacca cctcggcgac atcggcgtac tcttccttga gtggataggc gtacatgccc
     9961 aggccccggc cgctggcacc gaccagggcg tagcgtttgc gctgcttcat cctttttttc
    10021 ctcgttcctt agcgtgatcg ggcgcccgcg tttctacagg tacttcttga agaagtcgaa
    10081 ggcccggcga ccgctgaagg cgtgcggtcc gggatggatg tcggtccaga gatcggctgc
    10141 ggcctcggcg gcccggtaga tccgttccac gccccggtag ccctcgagct ggtcctggat
    10201 aaagaagcag gtgtcgtaga tgcccatttc cagcagcagt ggccgtgggg cgatgagtcc
    10261 ggcgatatcg tcggtgtcga agtatcggta gaggttgggg accatctggg agccgcacca
    10321 gttggcgcgc tcgatggcaa agccccgcca ggaattcaca tagccgatga tatcggcggc
    10381 ccggatgcgc ggttcgacgg ccgtggtgaa ggtggtcatg gtgccgccct gggagaggcc
    10441 catcatcccg atccggtccg gatcgacctc gggccgggtc tggagatagt cgacgcagca
    10501 cttcatgtcc cagatattca aggtcagcgt gtagacaccc agcatggctc ccttgagaaa
    10561 attgatattg cagggatcgc ggccggggaa ggggtcggcg ccgtcgcgcc gttcgccgaa
    10621 gacgcgcagg tcgggcgcca gggtgataaa gccggcccgg gccatctgct cggcgtaatt
    10681 ataattgtgc gcggcgatgt tctgctcgac ggccgccgag ctccggaatc cggccacggc
    10741 atccttgccg tggaggccgt ggccgtggct gcagagaatg gccgcctgct tacggtcggc
    10801 ggccatccgg cgcggcttga gcagtaggca gggaaccgac atgaacgcct cggaattgag
    10861 cactacccgt tcccggacca gatcgccctc ggttaccgag tattccacct cggcttgcaa
    10921 cggcaccggt ttcgggaaat cgcccagcag ttccagcagt ttcggcagcg cctttttccg
    10981 ccaggtccgc cagtcgttcc ggctccggcc ccggaaagcc agcaaaggtt tccagtcttc
    11041 gaatttccgg ttccagtaag actgcatcga gaaattccgt cgggcggaca tgtttcgata
    11101 gcctcggatt cgggttgccc gtttcaggcg ggccggtagt tgttgaccag gtcggcgacg
    11161 atcttcaccc ggtcgaaatc ggcgtcggat ggaacattgt cgctcatgcc gagcgcgaaa
    11221 aggtttccat gcggcacccg gggcagcagg tctttaatat aggcggccag gtcccggcgg
    11281 tcgccgccct gggggcacat caggac
//
LOCUS       DAOQYN010000038        11243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_6231_length_11243_cov_4.573114, whole genome shotgun sequence.
ACCESSION   DAOQYN010000038 DAOQYN010000000
VERSION     DAOQYN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11243
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6231_length_11243_cov_4.573114"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1281)
                     /locus_tag="PKN80_02705"
     CDS             complement(79..1281)
                     /locus_tag="PKN80_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ34955.1"
                     /translation="MALFQYSCLNPQGRRQKGRLEAHDAADARQKIKELGLYPIQVEM
                     AAGPDRPTLRPIRRIDHAEAVGAIRQLASLINSHIPVVEAVETVAPQLRNQPLSQSFH
                     NLSESLRRGILFSEALRRENIFPATLAEVVAVSEASGTLGLTLAEYARNEETALTMQR
                     RAVTSLIYPALLATVSILVLLFLFLVVLPKVTAVFAESGVPIPIYTRLIISAVLFLKR
                     YWFFMLLGLLATGLGLRQAWHGPAGRERLWRLVRRLPLIYELLRRRSLALFCRNLSIL
                     HRSGVKLIEALRLIERLMTDPAFRSEVQRLQNDLNRGRSLSQAMRSGPAFPPYLLQMV
                     RVGEETGDLSALLEKAAANYEEELETSLARAVGLLEPAMIIIMGIVVGSIVIAVLLPI
                     FEMSRIVR"
     gene            complement(1298..2953)
                     /locus_tag="PKN80_02710"
     CDS             complement(1298..2953)
                     /locus_tag="PKN80_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNQ34956.1"
                     /translation="MKIGELLVKRGLIPPPELEKAIRIQKEKPRLRLGEILLELGAVS
                     ERDLLQTLAEQFHLPFLENFEALFASDLATRFPFEFWQKWPSLPILKEERVYLVAADP
                     LRLPRQEIETRLETETRLAFGLPEEIQEVLSRNYWRPGQETLAPESKPETAVPGDGNG
                     SIRENLLDLANKAPVIRLVNQIIFQALQNNASDIHIQPQEKELRIRYRIDGMLHDAFQ
                     LPATMQPGIISRIKILSSLNIAERRLPQDGRTTVHLEDRAIDIRVSVLPTYWGEASVL
                     RLLDQASFFFTLEHLGLDQSELDIMQRLISADHGIILLTGPTGSGKTTTLYAALQKIN
                     YPDRNIVTLEDPVEYQIPGLSQIQVNPKIGLTFANGLRSILRHDPDVLMIGEIRDRET
                     AEMAIQASLTGHLVFSTLHTNDAASAITRLLEIGIEPYLVSSSLLGIIAQRLVRLICP
                     NCREAVPRDRLPAPVRDAHGTDLFRGRGCEKCFQTGYRGRTAIFEFLALDDHVRQSVL
                     NKQPAQEIKEYAVTKGMRTLRAAGEQKVRAGLTTLEEVLRVAS"
     gene            complement(2950..4638)
                     /locus_tag="PKN80_02715"
     CDS             complement(2950..4638)
                     /locus_tag="PKN80_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNQ34957.1"
                     /translation="MKKIRPVSSRASGFHPIRLLVALLLLISSLLLAAPAGRADSNFS
                     IDFNNVRLENFIQTMGRLLNQNFALAPGVSGSITIYSSRPIPVKEAESVFYAVLNLYG
                     FTAVPDPAGNVTKILPLAEARGRPLEVFAGRDPEQLVPFGDRYVTQIVPLSYAAPDAL
                     LPILNPFISKNANLSAETKTGVLIITDAASNIRKLLSIIDQVDREAPAGKDTLHIYRL
                     QNANAQEVAQVLTAVLAQKQARTPIRPGDKTVFVSVVAAAASNSLLITASPEDYASLE
                     KVITELDKMPGQVMIEALVAEVSGDLMREFGIQWQYFEPEAGKYRGFGSYGGVIDSAT
                     LSNIANGIAPPGLLVGLVKGNEFPFNIGALVRLYGKDSNFKILSTPQITTMDNKEAQI
                     KVAETIPYSKQITYSVTSEQIPTQSFDYQDVGITLKITPHISESRQVRLDIEQEVTKL
                     VSYLSSGGTSGSATILAPTIAKRTAKSSLIVSDQSTMVLGGLIRDDTDETVQKVPGLA
                     NLPLLGHLFRHQVKQNVQTSLYIFITPRIITNREEAETIADEKQAILEKPVRNR"
     gene            complement(4667..5491)
                     /locus_tag="PKN80_02720"
     CDS             complement(4667..5491)
                     /locus_tag="PKN80_02720"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNQ34958.1"
                     /translation="MNRYPRRRPRLIKDGTILLQGVLLLAGNYQSIYKRDLFRLAPPP
                     PPRTAEIKSIVRQPPSPAEYLLLKGVILADGLPSLAVIENRQARTEQVYPEGESIAEV
                     RILKIVKNKVILIDAENKETVLTLVAEAAGALTSSSGTAGLEKSPAKPAPPTVITINP
                     AEAGGPAFNSAYTVSLSEITQNVSASEELKQVRIAPVVNGGKVAGYQVNNLPANSIAF
                     RYGLRNGDVVSRVNGIQLENMSRLAFIYSTIRPGSPVSVEILRKGQPVTLNFNVTP"
     gene            complement(5454..6278)
                     /locus_tag="PKN80_02725"
     CDS             complement(5454..6278)
                     /locus_tag="PKN80_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ34959.1"
                     /translation="MKRLNFSVWVIFTLALFIPALVQAQTTSTGITGTNRSTSNRTSS
                     GSSSSSNTTASRLQDYKASVDEYAVRVTVRSMLETYAKNHPNIQVRIWSLIVLSKFGS
                     TIGSTDSGTTSSSTTTSSRRTTSSNTSSSDDDTSGDFEFYLSLPTLFQMLDDKEEVVR
                     LIASQTLQSSIDLSEEEWQRFMAPILINIAKTAKNPDVRIRCIKFISKLSKLNLSELN
                     SLPLLLDMAQNEDKEVRQSAMESLDEYLKQTQAPPPQAMEPGLTNEPIPAPPTPAY"
     gene            complement(6342..8249)
                     /locus_tag="PKN80_02730"
     CDS             complement(6342..8249)
                     /locus_tag="PKN80_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNQ34960.1"
                     /translation="MLKVKIGVKMTGLLALILLFPALVWAGPQAEALKAEEKAAAFYK
                     EGQSAYWTGNYAQALSLFSQVSAVSPRYKPLNVQIYQSLAAYRIKGKKNLPVVFPNPT
                     SSQSGFKELLINDEQEWLHLASEAESIILDVSTYLERLKNQALVPEEKLIDPTFYLRE
                     GRLALERNQFLEVIRYGQLAIEKLDAAFAETTAPTARLLGKIGDTPVTINVTDLDLKE
                     ALRQIYELTGVNIVLSSGITGTMTMNVKDVPLRQVLDLIVETHGLNYLERDNVIMIMT
                     PAEFEKTASGLALKEKKVFNLHYAEARAIAKIIRDSLRLENVTADLRTNSIVIDTGSA
                     TQTRDIENLIRSLDIPANEVLIEAQLLEVTYSNDRALGINFLIQNKLIDKVTLSGPRF
                     GSVPAALPIDSESVYFALTHKDFSAIFNALAKEGRARLIMSPRIMTVSGTPAVITSTE
                     DFPYTTSTVTQQPSATATNPLPPIITTQINTIQIGTTFQITPIIQNNRTVALNVSLLV
                     DRVKEIMQVPTSFGTSGQIIYQPYPLVASRSTDQNVVLWDGESLIVGGIGNVDTFNNE
                     TRVPVLGKVPVLGNLFKRTTRTERTGELVLFLKPRIVLTSEEGRLLNREYESLPVLQE
                     GPKQIIDRKWF"
     gene            complement(8311..>9890)
                     /locus_tag="PKN80_02735"
     CDS             complement(8311..>9890)
                     /locus_tag="PKN80_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34961.1"
                     /translation="QPAQPAPPTPAAPVLPKAAPVKAAAAAGKKDRQAENRLRLAAHL
                     EARRAAAGRAAAEKTNAAREKRAALELRRKDLAANIAARRQEKLASETHYREEKAAAE
                     AARQARMEARRREEAALKAKLEQERTAAAPVQPAQPTPPTPAAPVLPQAAPVKAAAAA
                     GKKDRQAENRLRLAAYLQEHRQKKGIPASSPVKVTLPPARIVPKPTPPLFLLKPPVPR
                     KPSALAARPVPVAPVSKKVIRPFDPVNFLKTNYIRAIFALLILGWFVEIFMLNQRLDQ
                     TRRQVEEALAPPAKSEAPEAPPEMLAQAPRPQVIRVEGLRDPFSHQLWRLREITSTPL
                     VRIVRIPNPPGLIRRIPIIRPPEPPVIPKPTFVAPNVPPAIPESEKISRIPPISVQLP
                     EVTLPAKPGLTLHFRGTLNLAGVDYFFLEGPDGGYRARVGDTVAGYKIYDYRNGVLYL
                     AREGHTYQINEERLSPPLRYKGRLIMAGEEYFFLEGQKTYRAIIGEEIEGFRLVRRIG
                     NYLYFVRDDRLYFLKQE"
     assembly_gap    9891..9990
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10566..10739
                     /locus_tag="PKN80_02740"
     CDS             10566..10739
                     /locus_tag="PKN80_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34962.1"
                     /translation="MLTVGLDLFEGGRAFFLAGSLLLCGQFPAAFYFGGLPFGFLAGR
                     FGFLPGQYLPAFV"
BASE COUNT         1845 a         3015 c         3792 g         2491 t
ORIGIN      
        1 cggagcgcca gtccgaccgc ggcgctggtc ttccccttgc cctcgccggt gtagacctgt
       61 gtcaatccgg ttcccatttc agcggacgat tcgactcatt tcaaaaatcg gcaggagcac
      121 ggcgatcacg atgctgccca ccacgatacc cattatgatg atcatggccg gttccaggag
      181 cccgactgcc cgggccaggc tggtttccag ttcctcctcg taattggcgg ccgctttttc
      241 gagcagggcg gagaggtctc ccgtctcctc ccctaccctc accatctgca gcaggtaggg
      301 cgggaaagcc ggcccggaac gcatggcctg gctgagcgag cggccccggt taagatcgtt
      361 ctgaagccgc tggacctcgg agcggaaggc cgggtcggtc atgaggcgtt cgatcaggcg
      421 aagcgcctcg atcagtttga ccccgctgcg gtggagtatg gagaggttgc ggcagaagag
      481 cgccagggat cggcggcgga ggagttcgta aatcaggggc agacgccgga ccaggcgcca
      541 gagccgttcg cggccggccg gaccgtgcca ggcctggcgc aggcccaggc cggtcgccag
      601 caggcccagt aacataaaaa accagtaacg cttgagaaaa aggacggcgc tgattataag
      661 ccgggtatag atgggaatcg gaacgcccga ttcggcgaag accgcagtca ccttgggcag
      721 cacgaccagg aagaggaaga gcaggaccag gattgacacg gtcgccagga gcgccggata
      781 gatcaggctg gtgaccgccc gccgctgcat ggtcagggcg gtttcctcgt ttcgggcgta
      841 ttcggccagc gtaaggccca gggtgccgct ggcctccgag acggccacca cttcggccag
      901 cgtggccggg aaaatgtttt cccgccgcag cgcctccgaa aaaagtatgc cccggcgcag
      961 tgattcggag agattgtgga acgactgaga gagaggttgg ttgcgcagct ggggggcaac
     1021 ggtttcaacc gcctccacta ccggtatgtg gctgttgatc agtgaggcca gctggcggat
     1081 ggcgccaacg gcttcggcgt ggtcgatgcg ccgaatcgga cgcagggttg gccggtccgg
     1141 gccggcggcc atttcaacct ggattggata gaggccgagt tccttgatct tctgacgggc
     1201 gtcggccgcg tcgtgggcct cgaggcgccc tttctgacgg cggccctggg ggttaagaca
     1261 gctgtattgg aataaggcca tggggaatca gaaaaattca ggaggcgacc cgcagcactt
     1321 cttccagggt cgttaagcct gcccggactt tttgctcgcc ggcagctcgc agggtgcgca
     1381 ttccctttgt taccgcgtat tcctttattt cctgggccgg ctgcttgttg aggacggatt
     1441 ggcggacatg atcatcaagg gccaggaact cgaagatcgc ggtccggccc cggtatccgg
     1501 tttgaaagca tttttcgcag ccccggccgc ggaaaagatc ggttccgtgc gcatcccgaa
     1561 ccggggcggg caagcgatcg cgcggtaccg cttcccggca gttggggcag atcaaccgga
     1621 ccaggcgctg ggcgatgatg cccagcagcg aggaggaaac caggtatggt tcgatgccga
     1681 tttccagcag tcgggtgatg gcgctggcgg cgtcgttggt gtgcagcgtc gagaagacca
     1741 ggtggccggt caggctggcc tggatggcca tttcggccgt ttcccggtcc ctgatttctc
     1801 cgatcatcag gacgtccggg tcatggcgga gtatggagcg cagaccattg gcaaaggtga
     1861 gaccgatttt gggattgacc tggatctggc tcaggccggg gatctggtac tccaccggat
     1921 cttccagggt gacgatattg cgatccgggt agttgatttt ctgcagggcc gcgtataggg
     1981 tggtggtctt gccgctgccg gtggggcccg tcagcagaat gatgccgtga tcggccgaga
     2041 tgagccgctg catgatgtcc agttccgact gatccaggcc gaggtgctcg agcgtgaaga
     2101 agaaggaggc ctggtcgagc agtctcagca ccgaggcttc gccccagtag gtgggcaaaa
     2161 ccgagacccg gatgtcaatg gcccggtcct cgagatgaac ggtggtgcgg ccgtcctgcg
     2221 gcagccgccg ctcggcgatg ttcagcgagg agagaatctt gatccgggaa atgatgcccg
     2281 gttgcatcgt ggccggcagc tggaaagcgt catggagcat accgtcgatg cggtagcgga
     2341 tcctaagctc tttttcctgg ggttggatat gaatgtcgct ggcgttgttc tggagcgcct
     2401 ggaaaatgat ctggttgacc agccggatga ccggtgcctt gttggccagg tcaagcaggt
     2461 tttcgcggat ggaaccattg ccgtcgcccg gaacggcggt ttccggtttg gattccgggg
     2521 ccagtgtttc ctggccgggg cgccagtagt tccgggagag cacttcctga atctcttcgg
     2581 gcaggccgaa ggccagccgg gtttcggttt ccagccgggt ctcaatctcc tggcgcggca
     2641 gccgaagcgg atcggcggcc accaggtaaa cccgttcctc tttcagaatc ggcagggacg
     2701 gccatttttg ccagaattcg aacgggaaac gggtggccag gtcggatgca aacagggctt
     2761 cgaagttctc cagaaaaggc aggtgaaatt gttcggccag ggtttgaaga aggtcccgct
     2821 cggaaacggc tcccagttcc agcagtattt cacccaggcg gaggcgcggt ttttcttttt
     2881 gaatccggat ggctttttcc agctccgggg gagggatcag gccgcgtttt accaggagtt
     2941 cgccgatctt catctgttgc ggaccggctt ttccagaatg gcctgttttt catcggctat
     3001 ggtttcggcc tcctcacggt tggtgatgat gcgcggggtt atgaagatgt agaggctggt
     3061 ctggacattc tgtttaacct ggtggcggaa gaggtgcccc aagagcggca ggttggccag
     3121 gcccgggacc ttctggaccg tttcgtcggt gtcgtcccgg ataaggccgc ccagcaccat
     3181 ggtcgactgg tcgctgacaa tcagactgct cttggcggtg cgcttggcga tggtcggggc
     3241 caggatggtg gcgctcccgc tggtgccgcc cgaagacagg taggagacca gcttggttac
     3301 ctcctgctcg atgtcgaggc gcacctggcg gctttcgctg atgtgcgggg tgatcttcag
     3361 cgtgatgccc acgtcctggt agtcaaaact ctgggttgga atctgttcgg aggtcaccga
     3421 gtaggtgatc tgctttgaat agggaatggt ttcggccacc ttgatctggg cttccttgtt
     3481 gtccatggtg gttatctgtg gcgtggaaag gattttgaaa ttgctgtcct tgccgtagag
     3541 gcggaccagg gcgccgatgt taaaggggaa ttcattgccc ttgaccaatc ccaccagcag
     3601 gccggggggc gcgatgccgt tggcgatgtt gctgagggtg gcggaatcga tcaccccgcc
     3661 gtaagatccg aaacctcggt acttgcccgc ctccggttcg aagtattgcc actggattcc
     3721 gaattcccgc atcaggtcgc cgctcacctc ggccaccagc gcttcgatca tcacctggcc
     3781 gggcatcttg tccagctccg tgatcacctt ttccagcgag gcgtaatcct cggggctggc
     3841 ggtgattaac aggctgttgc tggcggcggc ggctaccacc gagacgaaaa cggttttatc
     3901 cccggggcgg attggcgtcc gggcctgctt ctgggccagg accgcggtca gtacctgggc
     3961 cacttcctgg gcgttggcat tctggagccg gtaaatgtgc agcgtgtcct tgccggccgg
     4021 cgcttcgcgg tcaacctggt cgatgatact cagcagtttt cgaatgttgc tggcggcgtc
     4081 ggtgatgatc aggacgccgg tcttggtttc ggcggacagg ttggcgtttt tggagatgaa
     4141 aggattgagg atgggcagca gcgcatccgg agcggcataa ctcagcggaa cgatctgcgt
     4201 tacgtaacgg tcgccaaaag ggaccagttg ttccgggtca cgtccggcga agacctcgag
     4261 ggggcgtccc cgggcctcgg ccagggggag gattttggtc acgttgccgg ccggatcggg
     4321 gacggccgtg aatccataaa ggttgaggac cgcgtaaaaa acagattcgg cttctttgac
     4381 gggtatggga cgggaggagt agatggttat gcttccggaa acgccggggg ccagggcgaa
     4441 gttctggttg agaagccggc ccatggtttg aatgaagttt tccagacgta cattattgaa
     4501 gtctatggaa aagttggaat cggcccggcc cgccggagct gccaggagta agctggaaat
     4561 cagcagcaac aaggccacca gcagccggat cgggtggaag ccggaagcac gggaagaaac
     4621 cgggcggatt tttttcatca taggttttgg ttgcaatcga aaccggtcag ggcgtgacat
     4681 tgaaattcag ggtgaccggc tgccccttgc gcaggatttc aaccgacacc ggactgccgg
     4741 gccggatggt gctgtaaatg aaggcgaggc gggacatgtt ctccagctgg atgccgttga
     4801 cgcgcgaaac cacgtctcca ttacgcagtc cgtaacggaa ggcgatggag ttggccggca
     4861 ggttgttgac ctggtagccg gccaccttgc cgccgttcac caccggagcg atccggacct
     4921 gcttgagttc ttcgctggcc gagacgttct gggtgatttc gctcaggctg accgtgtatg
     4981 cggagttgaa ggccggaccg ccggcctcgg ccgggttgat ggtgatcacg gtgggcgggg
     5041 ccggtttggc cggcgatttt tcaaggccgg cggtcccgga agacgaggtc aaggctccgg
     5101 ccgcttcggc gaccagggtc aggaccgttt ccttgttctc ggcgtcgatc aggatgacct
     5161 tgttcttgac gatcttcaga atgcggacct cggcgatgct ttcgccttcc gggtaaacct
     5221 gctcggtccg ggcctgccgg ttttcgatca ccgccagtga aggcaggccg tcggccagga
     5281 tgactccctt gaggagcaga tattccgcgg gagacggcgg ctgccggacg atcgacttga
     5341 tttccgcggt ccttgggggt ggcggcggtg ccaggcggaa caggtcacgc ttgtatatgg
     5401 attggtaatt acccgccagc agcagaaccc cctggagaag gattgtcccg tccttaataa
     5461 gccggggtcg gcggcgcggg tatcggttca ttggttaggc ccggttccat tgcctggggc
     5521 ggcggcgcct gggtctgttt caggtattca tccaggcttt ccatcgcgga ctggcgcact
     5581 tctttgtcct cattctgtgc catgtcgagc aggagcggca gcgagttgag ttcgctcagg
     5641 ttaagtttgg agagcttgga aatgaacttg atgcaccgga tccgcacgtc agggttcttg
     5701 gcggtcttgg cgatgttgat aagaatcggg gccatgaacc gctgccattc ttcctcggaa
     5761 aggtcaatgc tgctctggag tgtctggctg gcgatcaggc gtacgacctc ctccttgtcg
     5821 tccagcatct ggaagagggt cggcaggctc aggtaaaact cgaagtcgcc gctggtgtcg
     5881 tcgtcgctgc tggaggtgtt gctgctggtg gtgcggcgcg aactggtggt ggtgctgctg
     5941 ctggtagtgc cgctgtcggt gctgccgatc gtactgccga acttggagag cacgatcagc
     6001 gaccagatcc ggacctggat gttcgggtga ttcttggcgt aggtctcgag catggaacgg
     6061 acggtcaccc ggacggcata ctcgtcaacc gacgccttgt agtcctgcag ccggctggcg
     6121 gtggtattgc tggaactgga actgccgctg ctggttctgt tgctggtgct gcggttagtg
     6181 ccggtgatgc ccgttgaggt ggtctgggcc tggaccaggg ccggaatgaa aagggccagg
     6241 gtaaagatta cccaaaccga gaaatttaac cgcttcatgc aagcctcctt gtttttccta
     6301 attagatgaa aagtggctgc gaattgttta tcgacccgaa tttagaacca tttccggtcg
     6361 atgatctgct tgggcccctc ctgcagtacc ggcagcgatt cgtattcgcg gttgagcagg
     6421 cggccttcct ccgaggtgag tacgattctc ggcttgagga agagaaccag ttccccggtt
     6481 cgctcggtcc gggtcgtccg cttgaagagg ttgcccagga ccggaacctt gcccaggacc
     6541 ggaacccggg tttcattatt gaaggtgtca acattgccga tgcccccgac aatcagactt
     6601 tcgccgtccc agaggacgac gttctggtcg gtgctccggc tggcgaccag cgggtacggc
     6661 tggtagataa tctggccgga agtcccgaag gaggtgggca cctgcatgat ctccttgacc
     6721 cggtcaacca gcaggctgac gttgagagcg accgtccggt tgttctggat gatcggggtg
     6781 atctggaagg tggtcccgat ctggatggtg ttgatctggg tggtgatgat gggcggcagc
     6841 gggttggtgg cggtggccga cggctgctgg gttacggtgg aggtggtgta gggaaaatcc
     6901 tcggtcgagg tgatgaccgc cggagttccc gaaacggtca tgatccgggg actcatgatc
     6961 aggcgggccc gcccttcctt ggccagcgca ttgaagatgg ccgagaaatc cttgtgggta
     7021 agggcgaagt agaccgattc cgagtcgatc ggcagggcgg ccgggaccga accgaaacgc
     7081 ggcccgctca gggtgacctt gtcgatgagc ttgttctgga tcaggaagtt gatgcccagg
     7141 gcccggtcgt tggagtaggt aacctcaagc agctgggcct cgatcagcac ttcgttggcc
     7201 ggtatgtcca gggagcggat caggttttca atgtccctgg tctgggtggc gctgccggtg
     7261 tcgatgacga tcgagttggt gcgcaggtcg gcggttacgt tctccaaccg gagcgagtcg
     7321 cggatgatct tggcgattgc cctggcctcc gcgtaatgga ggttgaagac ctttttttcc
     7381 ttcaaggcca ggccgctggc ggttttctcg aattcggccg gggtcatgat catgatgacg
     7441 ttgtcccgtt ccaggtagtt gagcccgtgg gtttccacga tcagatccag gacctggcgc
     7501 agggggacgt ccttgacgtt catggtcatg gtgccggtga tgcccgaaga gaggacgatg
     7561 ttgacgccgg tcaactcgta gatctggcgg agggcctcct tcaggtcaag atcagtcaca
     7621 ttgatggtaa ccggggtgtc gccgatcttt cccagcaggc gggcggtcgg ggcggtggtc
     7681 tcggcgaagg ccgcatcgag tttctcgatg gccagctggc cgtaacggat cacctccagg
     7741 aactggttcc gttcgagggc cagccgccct tcgcggagat agaaggtggg atcgatcaac
     7801 ttttcctcgg ggacgagcgc ctggtttttg agccgttcga gataggtgga aacgtccagg
     7861 attatggatt cagcctcgga ggccaggtgc agccattcct gctcgtcgtt gatgaggagt
     7921 tccttgaatc cggactgact ggaagtcggg ttgggaaaga caaccggcag gttctttttg
     7981 cccttgatcc ggtaggcggc caggctctgg tagatctgaa cgttaagcgg cttgtaccgg
     8041 ggggagaccg ccgacacctg ggagaagagg gaaagggcct gggcgtaatt gccggtccag
     8101 taagctgatt ggccttcctt gtaaaaggcg gccgccttct cctcggcttt cagcgcctcg
     8161 gcctggggcc cggcccagac cagggctgga aagagcagga tgagcgcgag caatccggtc
     8221 atcttaacgc cgatcttcac tttcagcatt tgtaatctcc tccttaaagt atatactcta
     8281 aagaacccgg tgaagtcaag gttttatcgg ttattcctgc ttcaggaagt acaggcggtc
     8341 atcacgcacg aagtagagat agtttccaat gcggcgaacc aggcggaagc cttcgatttc
     8401 ctcgccgatg atggcgcgat aggttttctg accttccagg aagaagtatt cttcgccggc
     8461 catgatcagg cgtcccttgt aacgcagcgg cggactcagg cgttcctcgt tgatctggta
     8521 ggtgtgtcct tcccgggcga gataaaggac gccgttgcgg taatcatata ttttgtatcc
     8581 ggccaccgtg tctcccaccc gggcccggta acctccgtcc ggcccctcca ggaagaagta
     8641 gtcgacgccg gccaagttga gtgtgcctcg aaaatgaagc gtcaagccgg gcttggccgg
     8701 cagagtcacc tccggcaact ggaccgagat gggcggtatc cggcttattt tttcgctctc
     8761 cggtatggcc ggcgggacgt tcggcgcgac aaaggtcggc ttgggaatca cgggcggttc
     8821 cggcggcctg atgatcggga tgcgtcttat cagaccgggc ggattcggta tccggacaat
     8881 gcgaaccagt ggcgtcgaag taatttctcg caaccgccag agttggtgtg agaatggatc
     8941 acggaggccc tcaacccgga tgacctgggg tcttggagcc tgcgccagca tttccggcgg
     9001 tgcttcgggg gcttcgctct tggccggagg ggccagcgct tcctcaacct ggcgtcgggt
     9061 ctggtccaga cgctggttga gcataaagat ttcgacgaac cagcccagga tcaagagcgc
     9121 aaagatggcc cggatataat tggtcttcag gaaattgacc gggtcaaacg gccggatgac
     9181 cttttttgat acgggcgcta ccggtaccgg ccgggcggca agcgcggatg gttttctcgg
     9241 aaccggaggc ttcaacagga aaagcggcgg tgtcggtttc ggaacgatcc gggccggcgg
     9301 cagggtaact ttaaccgggc tggacgccgg gattcctttt ttctggcggt gttcctgaag
     9361 gtaggcggcc agtcggagcc ggttctcggc ctggcggtct ttctttccgg cggcggccgc
     9421 ggcttttacc ggtgcggctt gcggaagaac gggcgcggcc ggggttggcg gcgtgggttg
     9481 ggccggctgg accggggcgg cggccgtccg ttcctgctca agtttggctt ttaaggcggc
     9541 ctcctcgcgg cggcgcgctt ccatccgggc ctgccgagcg gcttcggcgg cagccttctc
     9601 ttcccggtaa tgggtttcgg aggccagctt ttcctggcgt cgggccgcga tgttggcagc
     9661 caggtctttg cggcgcaatt cgagtgcggc ccgtttttcc cgggccgcgt ttgtcttttc
     9721 ggcggcagcg cggccggcgg cggcccgcct ggcttcaagg tgggcggcca gccggagtcg
     9781 gttctcggcc tggcggtctt tctttccggc ggcggccgcg gcttttaccg gtgcggcttt
     9841 cggaagaacg ggcgcggccg gggtcggcgg cgcgggttgg gccggctgga nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gctttcggaa gaacgggcgc ggccggggtc
    10021 ggcggcgcgg gttgggccgg ctggaccggg gcggcggccg cccggtcctg ttcaagcttg
    10081 gcttttaaga aagtttcctc gcggcggcgc gcttccatcc gggcctgccg ggcggcttct
    10141 tcttcccggc gtctggtttc cgcccgcgtc tttctatcaa gttcggcccg gaccgccgtc
    10201 tctctcttct gtctgcgatc agcctccctt gattctttaa ttagtttttt tgattcccgg
    10261 gctgcctgga ggacgcggtt atgttcggcc tgcccttcga cttcgcggcg gatcttttcc
    10321 gcccgagcct gtagtttcga tgaacgggat tctttcaggt aggcggccag gcgggtccgg
    10381 ttgctttgct gccggttcgt tctttctggt aactcggtgg ttccggtttc cggagttttt
    10441 agagttgcga cagggcgtgc gccacggttt tcagctttct tttcccggcg tgcccgggcc
    10501 tcggtttccc tggctttccg ggccgattgg gcggcttctc gacggcgggc cgcttccagc
    10561 gaggcttgct gacggtgggc ctggatttgt ttgagggcgg ccgcgcgttt ttcctggcgg
    10621 gcagtctcct gttgtgcggc cagttcccgg cggcgtttta tttcggcggc cttccgttcg
    10681 gatttctggc gggccgcttc ggctttttgc cgggccagta tctcccggcg tttgtttgac
    10741 tggtttcttc ccggggtttc ggtccccggc tgtccggttg gcttttcttc acccccggga
    10801 tcgccaggcg aactcctgtc cccggaagaa ccggtttggg gtgactgggg ttgagccgac
    10861 ccggaataac ccgttttcgg cttggatgaa ggaatgattg gccggcttga ggccggggat
    10921 gatttggaat tattatgccg gcccgactca gccttgaccg ggccggaagg gttgggcgga
    10981 attcttctgg cccgggccgg atcgggaatt gtccggtttc gagcggtgcc gttgctccgg
    11041 ttcttctcgc gttggctgga aagccacgcc cgcatttgcg ttcttttttc ttcccggttc
    11101 atgttgttgg ttggtttcct gtttttttta aggtccggtc ggcggcgctt cggggggcgt
    11161 cggtggcatc tccggggccg gggccccagg tgccggtccg gccggctggg ctgtctttct
    11221 gcaatgcggc cggtccttcc tcc
//
LOCUS       DAOQYN010000039        11129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_6297_length_11129_cov_4.263049, whole genome shotgun sequence.
ACCESSION   DAOQYN010000039 DAOQYN010000000
VERSION     DAOQYN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6297_length_11129_cov_4.263049"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4362)
                     /locus_tag="PKN80_02745"
     CDS             complement(<1..4362)
                     /locus_tag="PKN80_02745"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNQ34963.1"
                     /translation="MPRSRIAVTLIVLAVMAAGTFFTMSAFGQRGNPADRERAARLMK
                     DGNFKEAFDIYSALALDPADAPEKVSEDLSAAVQCLRNLGRVSEFDAFIEKVVKAHGK
                     NWRLLARAGELYQEIEHYGSMVAGEFVRGPHRGPAVMMNSFERDRVRSLQLYREAIEK
                     KEGNPAAANDGQTGYLYLTCYQALLGNRGWSEAWRLQYLTDLTVLPDYEEGYYRGDYR
                     SGTRGAPVNPDGTPVFHRLPKSWEAAATDGERFRWCLAQAIELSPSLKGRTLYEWADF
                     LCNQFDVATMSGYRGYFGQLAEDDTKKNESGPYAVHTLGEDETIARLANGIKRFKLPD
                     EFNYIRIYKQIADEDRAGYGEQALLRLTSVHENRMQYDAAAGYLRRSIREHGDRGQDK
                     QNRLDQIVGNWGQFQPAATQPAGVGATVDYRFRNGRSVSFTAREVNVAKLLADVKEYI
                     KSRPKTLEWEQVNIENLGYRLVTKKQEQYVGRQVAAWELPLEPRPKHFDRRITVTTPL
                     QKPGAYLLTAKMNGGNTSSIVLWVADTAIVKKPLENGTYYFVADAVTGQPVAGANLEF
                     FGYWQKWREVRRTGEAQYDIQTRNFAERADPDGQVIVKGSDEDRNYAWLITATTGDGR
                     FAYLGFTHAWFGRYYDAEYNQVKHYLITDRPVYRPEQTVKFKAWVNNARYDQEGPSPF
                     AGRKLQVQINDPRGEKVFEKEYTADEYGGIDGEFPLAEDAMLGQYGVRIANQGYGTFR
                     VEEYKKPEFEVKVDAPAEPVMLGEKITARVEAKYYFGAPVTEAKVRYKVSRSSHSAEW
                     YPPLRWDWFYGRGYGWFGYDYAWYPGWKEWGCGRPWPWWWSRRPEPPELVAEAEAPIG
                     PDGTIEIPIDTGPAKEMLGDTDHRYEISVEVTDQSRRTITGQGTVLVARKPFKVYAWT
                     NRGHYRAGDAVRVQACGRTLDGKPVQGKGLLRLLRVRYPKGEPEETEIQRWNLDTDEE
                     GKALQQIKAAQAGQYRLSYRLTDARGHLIEGGYVFCVTGPGLAGDDFRFNDIELIPEK
                     TEYAPGETADLMINTNRAGGCVLLFLRPANGSYLAPKVIRLQGKSALERIEVIKKDMP
                     NFFVEAVTVSGGRVFQETRELAVPPESRVLNVSVEPSAEKYRPGEKGTLKFQVTDASG
                     EPYAGSLAVTLYDKAVEYISGGSNVPEIKEFFWKWRRHHQPSQETNLSRSGYDISDRS
                     MGYLGIFGHLAENEEMLSSNRMAEGRGGAVAMRASKSMSGAMMEMAAAPAPSMALGMD
                     AVEKEQKDEQMGGGAAPMAEPSVRTRFADTAFWAAAISTDRQGRAQVEVTMPENLTTW
                     KARVWAMAAGSRVGEGSAEVITTKNLIIRLQAPRFFVQKDEVVLSANVHNYLKTAKKV
                     EVILEMEGGCLALMPGSTLKKTVSVEANGEKRVDWRVKVTQPGTAVVRMKALTDEESD
                     AMQLSFPAYVHGMLKTES"
     gene            complement(4542..6620)
                     /locus_tag="PKN80_02750"
     CDS             complement(4542..6620)
                     /locus_tag="PKN80_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34964.1"
                     /translation="MKKRINKWLRISVLTCLAAGVIGLGRPAAAAAGNLNQKTEGSRR
                     IVEGDFFRITWDGRQGGQISEILLHDGIAWRSILPKGAALPALEIVSGGKKYTLAGDT
                     GASFRAGTADQSGEEIQFTFAGELKGPDGTAPGVSARQAFTLHRNGVLFCDLELKFNR
                     ALAVEAASLGLRLDKSAQPTLRREKVTRHGLILWSGDAPKWEPVKAVRYPDPYVLFSL
                     ADEAGGRPWTNHVDFWVEGRDALGGTGYIQRKPLEAGEFGHTWNLAAAGGIQAAAGTV
                     YRNRWGLGLGRAVTGGRLIGARVYHWLGRHGDKPFLPTDEALKRMADDGANILILHDD
                     WVADHRPMHTYRPYNEEEAKDLDRLIASAHKLGLRLMVYLRPYPHLLADNGPWKSRFQ
                     RDWDGIYIDHASTEEFSEYAMVFGTPALDYAFAAKQYLRYTQELRRTVGDGGFLIMHT
                     GAAEHGFGHAVVDAYLSGESRTRMLESPRVCVEGMMPSAAVPTIWTWRPEQRPYLTPR
                     AVAYWAGLGAFPHVTFHHENALFPEAGGYKPMPKENQPAPISDSRGRSYIWPLWQIWR
                     TLPFPYTGEYYTRNNHAPVTVIRPADRTVETTVYQPDGESLLVIVSNLAEETVPGVTL
                     DLEKSLKIPAGSRLVEMTASGFDLKCRVAAQTFSGRRIAVGELGPYQYKGYLLTKKAL
                     PAHLQALLEP"
     gene            complement(6832..7692)
                     /locus_tag="PKN80_02755"
     CDS             complement(6832..7692)
                     /locus_tag="PKN80_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNQ34965.1"
                     /translation="MRWQGRRRSSNVEDLRGSSGNRALRVGGGLSGIGVLIALAYLFL
                     GGNPAEVGRIFQGAPSYSVESGQELTEAEKEMGEFVSTVLADLEDVWQKEFRQMGLTY
                     EEPALVLFSGAVETAAGYASASTGPFYSPADSKVYIDLTFFEEMQRQLNAPGDFALAY
                     VLAHEVGHHVQNLLGINEKVMSQRNRMSEKEFNRLLVRLELQADFLAGVWAHYARQIP
                     DYLEATDIEEGLNAASAVGDDRIMKQSQGYVVPDAFTHGTSEQRVRWFRKGLETGDIN
                     QGDTFGAARL"
     gene            complement(7774..8526)
                     /locus_tag="PKN80_02760"
     CDS             complement(7774..8526)
                     /locus_tag="PKN80_02760"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNQ34966.1"
                     /translation="MAMVRIKDELARKRVLLGISICYPVPGIIELVGRDWDWIWIDGQ
                     HGEHDYNSILGCVRACELVRRPSVVRVPSHDYGFIGLALDTNTSGIMVPMVNNARQAE
                     SIVQAAKFPPLGERSYGGRRPVDMHGRAYSHTANEDTLLIAQIETAEGLEQAGEIAAV
                     QGVDAILFGADDLALRASMPMDQPRHPETFRKEMEKVAQAAKETGKIAGVVTPSPDLY
                     RLAVEMGFRLCVGVGEVGILAEGSKNARERLG"
     gene            complement(8640..9128)
                     /locus_tag="PKN80_02765"
     CDS             complement(8640..9128)
                     /locus_tag="PKN80_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34967.1"
                     /translation="MPVRIRLRPYHPTFIVAHYGGGIPQESRARAGFESIQRKIRENP
                     LEEIEIEIVRGHDDACESCKFRRESETGSIWGKRHTCTSAEDPHMVAEVERINNKVFG
                     LLGLDYGSVVNLGTLVFRLSEKIPVLDDNMLGGLDKQEAYRKGLAELLARREQETGRT
                     GK"
     gene            9282..10127
                     /locus_tag="PKN80_02770"
     CDS             9282..10127
                     /locus_tag="PKN80_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34968.1"
                     /translation="MKKKFAGGWIFILKGFPGWNQGRKQVKKAVLLLFLLVLFAGLGR
                     AAAGIEPGPEVVLWAWERPENLLFLKDGRVRVAFYAGSVTFAGSQTLFLPRRQPLLVL
                     PAVPLTAVFRIENRQAAAPGGRQLSESIEVIARSFAGRGITGLQLDFDCRASERPFLE
                     GLVEGLKRRLPPSTALSVTVLASLSHAGSWIDTLPVDEAVPMLFRMGPDDYLVRKQLA
                     GRSFLKAAACRKAVGLSLDEPLPPAAYLKERRIYLFNPHPWTGEALDRARLEIRRRLE
                     EDNSP"
     gene            10124..>11129
                     /locus_tag="PKN80_02775"
     CDS             10124..>11129
                     /locus_tag="PKN80_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34969.1"
                     /translation="MKGSGPKFRFLLFFPLLLLVAAPASACGPFFPHYLYTPEDIAEV
                     GSPYSYNRNFSGASPGIIFPAWRTSVLYEVYRGLSGRLPVESLSAAPVDEPADPAGWL
                     AARKTVTETAVEIRDWAEGENYSYYQNYLADAFGTAARTLSERKPVFTPEELKLWLAG
                     QDEVFGSAPDRLFDAELDRSISNPLLRQDREYQRAAACFYRRKYDEAEARFREIAGNP
                     GHPWRVYAALAAGRTLIRRAGLEYELEFKKSGGGGEGDKKALAIRNEWLLKARAQLEG
                     ILKDATLRTVHPSARRLLDYIVFRLEPRERLLAAEKVLLLSEDAAEIRMNLRDFALLH
                     G"
BASE COUNT         1633 a         3731 c         3556 g         2209 t
ORIGIN      
        1 gctctcggtc ttcagcatcc cgtgcacgta ggccgggaag gagagctgca tcgcgtccga
       61 ttcttcatcg gtgagggcct tcatccggac gaccgccgtg ccgggctggg tcacttttac
      121 ccgccagtcc acccgctttt cgccgttggc ctccacggaa accgtcttct tcagcgtcga
      181 gcccggcatc agcgccaggc aaccgccctc catttccagg attacctcaa ccttcttggc
      241 ggtcttcagg taattgtgga cgttggccga gaggaccacc tcgtccttct ggacgaagaa
      301 gcgcggcgcc tgcagccgga tgatcaggtt cttggtggtg atcacctcgg ccgaaccttc
      361 gccgacgcgg ctgccggcgg ccatcgccca gacccgggcc ttccaggtgg tcaggttctc
      421 cggcatcgtt acctcgacct gggcccggcc ttgccgatcg gtggagatgg ccgccgccca
      481 gaaggcggtg tcggcgaacc tggtgcgcac ggacggttcc gccatcgggg ccgccccgcc
      541 tcccatctgt tcgtcttttt gctctttctc cacggcgtcc atccccaggg ccatcgaagg
      601 agccggtgcg gccgccatct ccatcatggc gccggacatg cttttggagg cccgcatggc
      661 cacggcgccg ccccggcctt ccgccatccg gttgctggag agcatctcct cgttctcggc
      721 caggtggccg aagatgccga gatagcccat gctccggtcg gagatgtcgt agccgctccg
      781 gctgaggttg gtttcctgcg agggctggtg gtggcggcgc cacttccaga agaactcctt
      841 gatctccggg acgttggagc cgcccgaaat gtactccacc gccttgtcgt aaagcgtcac
      901 cgccaggctg cccgcgtacg gttccccgga ggcgtcggtt acctggaatt tcagcgtgcc
      961 cttctcgccc ggccggtact tctccgcgga tggctccacc gagacgttca gcacccggct
     1021 ctccggcggc acggccagtt cgcgcgtttc ctggaagacc cggccgccgc tgaccgtcac
     1081 cgcctcgacg aaaaagttcg gcatgtcctt cttgataacc tcgatccgtt cgagggcgct
     1141 cttgccctgc aggcggatga ccttcggcgc caggtagctg ccgttggccg gccgcaggaa
     1201 gagcaacacg cagccgccgg cccggttggt gttgatcatc agatcggccg tctcgcccgg
     1261 cgcgtactcg gtcttctccg ggatcagttc gatgtcgttg aagcggaaat cgtcaccggc
     1321 cagccccggt ccggtgacgc agaagacgta accgccctcg atcagatggc cgcgggcgtc
     1381 ggtgagccgg tagctgagcc ggtactggcc ggcctgcgcg gccttgatct gctggagcgc
     1441 cttgccttcc tcatcggtgt ccaggttcca gcgctggatc tcggtctcct ccggctctcc
     1501 cttcgggtag cggacccgca gtaggcgcag gaggcccttg ccctggaccg gtttgccgtc
     1561 cagggtccgg ccgcaggcct gcacccggac ggcgtcgccc gcccggtagt ggccccggtt
     1621 ggtccaggcg tataccttga agggcttgcg cgctaccagc accgttccct ggccggtgat
     1681 cgtgcggcgc gactggtcgg tgacctcgac gctgatttcg tagcggtgat ctgtgtcccc
     1741 gagcatctcc ttcgccgggc cggtgtcgat tggaatctcg atggtgccgt ccgggccgat
     1801 cggcgcttcc gcctcggcca ccagttccgg cggctccggc cggcggctcc accaccaggg
     1861 ccaggggcgg ccgcatcccc actctttcca gcccgggtac caggcgtaat cgtagccgaa
     1921 ccagccgtag ccacggccgt agaaccagtc ccagcgcagc ggcgggtacc actcggctga
     1981 gtgggaggag cgggaaacct tgtagcggac ctttgcctcg gtgaccggcg cgccgaagta
     2041 gtacttcgcc tccacccggg cggtgatctt ctcgcccagc atcaccggct cggccggcgc
     2101 gtccaccttg acctcaaact ccggcttctt gtactcctcc acccggaagg ttccgtatcc
     2161 ctggttggct atccttaccc cgtactggcc gagcatggcg tcttcggcca atgggaattc
     2221 gccgtcgatc ccgccgtact cgtcggcggt gtactccttc tcgaaaacct tttcgccccg
     2281 gggatcgttg atctgcacct ggagcttccg gcccgcgaac ggggagggtc cttcctggtc
     2341 atacctggcg ttattgaccc aggccttgaa cttgacggtc tgctccggtc ggtagaccgg
     2401 ccggtcggtg atcaggtaat gcttgacctg gttgtactcc gcgtcgtaat agcggccgaa
     2461 ccaggcgtgg gtaaagccga ggtaggcgaa gcggccgtcc ccggtggtgg cggtgatcag
     2521 ccaggcgtaa ttccggtcct cgtccgaccc cttgacgatg acctggccgt ccgggtcggc
     2581 ccgctccgcg aagttgcgcg tctggatgtc gtactgggcc tcgccggtcc gccggacctc
     2641 ccgccacttc tgccagtagc cgaagaactc caggtttgcc ccggccaccg gctggccggt
     2701 gaccgcgtcg gcgacgaagt agtaggtgcc gttctccagc ggcttcttga cgatggcggt
     2761 gtcggcgacc cagaggacga tgctgctggt gttgccgccg ttcatcttcg cggtcagcag
     2821 gtaggcgccc ggtttctgca ggggcgtggt gacggtgatg cgccggtcga agtgtttcgg
     2881 ccggggttcc agcgggagtt cccaggccgc gacctgtctc ccgacatact gttcctgctt
     2941 tttggtgacc agccggtagc cgaggttctc gatgttgacc tgctcccatt caagggtttt
     3001 cggccgtgac ttgatgtact ccttgacatc ggccagcagt ttggcgacgt tgacctcgcg
     3061 ggcggtgaag ctcaccgagc ggccgttgcg gaagcggtaa tccacggtgg cgccgacgcc
     3121 ggccggctgg gtggccgccg gctggaactg gccccagtta ccgacgatct ggtccaggcg
     3181 gttctgctta tcctgtcccc ggtcgccgtg ctcccggatg ctgcggcgca ggtacccggc
     3241 ggcggcgtcg tactgcatcc ggttctcgtg gacgctcgtc agccggagca gcgcctgctc
     3301 cccgtagcca gcccggtcct cgtcggcgat ctgcttgtag atcctgatgt aattgaactc
     3361 gtcgggaagc ttgaagcgct tgatcccgtt ggccagccgg gcgatcgttt cgtcttcgcc
     3421 cagggtatgc accgcgtagg ggccgctttc gttcttcttc gtgtcgtctt cggcgagctg
     3481 gccgaagtag ccgcggtatc cgctcatcgt ggccacgtcg aactggttgc agaggaaatc
     3541 ggcccactcg taaagggtcc ggcccttgag tgagggcgac agttcgatcg cctgggccag
     3601 gcaccagcgg aatcgttcgc cgtcggtggc ggccgcctcc cagctcttcg ggagccggtg
     3661 aaagaccggc gtgccgtctg ggttgaccgg cgccccgcgc gttccggacc ggtaatcgcc
     3721 ccggtagtag ccctcctcgt agtcgggcag gacggtcagg tcggtcaggt actgcaggcg
     3781 ccaggcctcg ctccagccgc ggttgcccag cagggcctgg tagcaggtga ggtagaggta
     3841 gccggtctgg ccgtcgttgg ccgcggccgg gttcccttcc ttcttctcga tggcctcccg
     3901 gtagagctgc agggaacgca cccggtcccg ctcgaatgag ttcatcatca ccgccggtcc
     3961 ccggtgcgga ccgcgtacga actccccggc caccattgag ccgtaatgtt ctatttcctg
     4021 gtacaactcg ccggcccggg ccagcaggcg ccagtttttc ccgtgggcct tgacgacctt
     4081 ctcgatgaag gcgtcgaatt cgctgacccg gccgagattg cgcaggcact gcacggcggc
     4141 cgacaggtcc tcggagacct tctccggggc gtcggccggg tcgagcgcca gcgcgctgta
     4201 gatgtcgaag gcctccttga agttgccgtc cttcatcaac cgggcggccc gctcccggtc
     4261 ggccgggttc ccccgctggc cgaaggcgct catcgtgaag aaggttccgg cggccattac
     4321 ggccagtacg attaacgtga cggcgattct tgacctcggc atgatctgct ccttcctgga
     4381 attgagggga ccggttgaac gttgctccgg cggtctcagc cgtttcccga ttctcccggc
     4441 ggtccgtttg tcacattaga caccggggcc ggtaaaatgt tccggtccgg tccgcataac
     4501 ctgcggcccg gcttggttcc gccccctccg tccggccgcc ttcagggttc gagcagcgcc
     4561 tggagatggg ccggaagcgc ttttttggtc agcaggtatc ccttgtactg gtagggtccg
     4621 agttcgccca ccgcgatccg gcggcccgag aaggtttgag ccgccacccg gcatttcagg
     4681 tcaaagccgc tggccgtcat ctccaccagc cggctgccgg ccgggatctt cagggatttc
     4741 tccaggtcga gcgtgacgcc gggaacggtc tcttcggcca ggttggagac gatgaccagc
     4801 aggctttccc cgtccggctg gtagaccgtc gtttccaccg tccggtcggc cggccggatc
     4861 accgtcaccg gcgcgtggtt attgcgcgtg tagtactcgc cggtgtaagg gaagggcagg
     4921 gtgcgccaga tctgccagag cggccagatg tagctgcggc cgcgggaatc ggagatcggc
     4981 gccggctgat tttcctttgg catcggctta tagccgcccg cctccgggaa gagcgcgttc
     5041 tcgtggtgga aggtaacgtg cggaaaggcc cccaggccgg cccagtaggc gaccgcccgc
     5101 ggggtcaggt aggggcgctg ctcgggccgc caggtccaga tggtcggcac ggccgcgctg
     5161 ggcatcatcc cctccacgca gacccggggc gactcgagca tcctggtccg ggattcgccg
     5221 ctcaggtagg cgtccacgac cgcgtgaccg aagccgtgct cggcggcgcc cgtgtgcatg
     5281 atcaggaagc cgccgtcccc gaccgtccgg cgcagttcct gggtatagcg taagtactgc
     5341 ttggccgcga aggcgtaatc gagggccggc gtcccgaaga ccatcgcgta ctcggagaac
     5401 tcctcggtgg aggcgtggtc aatgtagatg ccgtcccaat cgcgctggaa ccggctcttc
     5461 caggggccgt tatcggccag caggtggggg taggggcgca ggtagaccat caggcggagg
     5521 cccagcttgt gggccgaggc gatcaggcgg tccaggtcct ttgcctcctc ctcgttgtag
     5581 ggccggtagg tgtgcatcgg ccggtggtcg gccacccagt cgtcatgcag gatcaggatg
     5641 ttggcgccat catccgccat ccgcttcagc gcttcgtcgg tgggcaggaa cggcttgtcc
     5701 ccgtggcggc ccagccagtg gtagacccgg gcgccgatca ggcggccgcc ggtcaccgcc
     5761 cggcccagtc cgagtcccca acggttgcgg tagaccgtcc cggccgccgc ctggatgcct
     5821 ccggcggccg ccaggttcca ggtgtgtcca aattccccgg cctcgagcgg cttccgctgg
     5881 atgtatccgg tcccgccgag ggcatcccgg ccttcgaccc agaagtcaac gtggttggtc
     5941 caggggcggc cgccggcctc gtcggccagc gagaagagga cataagggtc cgggtagcgg
     6001 acggccttga ccggttccca cttcggggcg tccccggacc agaggatcag gccgtgccgg
     6061 gtaaccttct cccgtcgcag cgtcggctgg gcgctcttgt ccagccggag gcccaggctg
     6121 gccgcctcga ccgccagggc ccggttgaac ttcagttcca ggtcgcagaa gagcaccccg
     6181 ttgcggtgga gcgtgaaggc ctgccgggcg ctcacgcccg gggcggttcc gtccggcccc
     6241 ttcagttccc cggcgaaggt gaattgtatc tcttcgccgc tctggtcggc cgtcccggcc
     6301 cggaaactcg ccccggtatc gccggccagg gtgtattttt taccgccgct gacgatctcc
     6361 agggccggca gggccgcccc tttcgggagg attgaccgcc aggcaatgcc gtcgtgcagg
     6421 agtatctccg agatctggcc gccctggcgg ccgtcccagg tgatgcgaaa gaaatctccc
     6481 tccacgatcc ggcggctgcc ttccgtcttc tggttgagat tgccggcggc cgcggcggcc
     6541 ggccggccca gtccgatcac gcccgcggcc aggcaggtca gcaccgatat ccgcaaccat
     6601 ttgtttattc tttttttcat catgtcctcc cgggcgagtt gaatgattcg gattcagcgg
     6661 gggaagtttt ttctttgacg ccgggtcccc ggatcccggc gccggtcctc cccagtattt
     6721 tttttctgga aaacgggaag gcgcctccgg tccttgatcc gggctggttc ccagcatcgc
     6781 ttctttcaac tgcacgccat ccaggacggc cgcggattga tcgggtcgcc gctaaagccg
     6841 ggcggcgccg aaggtgtctc cctggttgat atctccggtt tcgagcccct tgcggaacca
     6901 gcggacccgc tgttcggagg tgccgtgggt aaaggcgtcc ggcactacgt aaccctgtga
     6961 ctgcttcatg atccggtcgt ctccgaccgc gctggcggcg ttgagccctt cctcgatatc
     7021 cgtcgcttcg aggtaatccg ggatctgcct ggcatagtgg gcccagactc cggccaggaa
     7081 gtcggcctgg agctccagac ggaccaggag ccggttgaat tccttttcgc tcatccggtt
     7141 gcgctggctc atgacctttt cgttgatgcc cagcaggttc tggacgtggt ggccgacctc
     7201 gtgggccagc acgtaagcca gagcgaaatc accgggcgcg ttcaattggc gctgcatctc
     7261 ctcgaagaag gtcaggtcga tgtatacctt ggaatccgcc ggggagtaga agggtccggt
     7321 cgaggcgctg gcatagccgg cggccgtttc gaccgcgccc gagaagagaa cgagggcggg
     7381 ctcctcgtaa gtcaaaccca tctgccggaa ctctttctgc cagacatcct cgaggtcggc
     7441 caggaccgtg gagacgaact cccccatttc cttctccgcc tcggtgagtt cctggcccga
     7501 ttccaccgag taggacggcg cgccctggaa aatccggccg acctcggccg ggttgccgcc
     7561 caggaagagg taggcgaggg cgatcagtac gccgatgccg ctcagtccgc ctccgacgcg
     7621 gagggcccgg ttacccgacg aaccgcgcag gtcctcgacg ttactgctcc ggcgccttcc
     7681 ctgccatctc atcttttggt ctcctttatg cgcatggttc gcccggccct ggtgccggcc
     7741 ggcttcgtgg attccccggc cgccggccgg ggatcacccg agtcgttccc gggcattttt
     7801 agaaccctcg gccaggatac cgacctctcc aactcccacg cagaggcgga accccatttc
     7861 tacggccagc cggtaaagat ccggcgaagg cgtgactacg cccgcgatct tgccggtttc
     7921 tttcgccgcc tgcgcgacct tttccatctc tttccggaag gtttccggat ggcggggctg
     7981 gtccatgggc atcgaggccc gcaaggccag gtcgtcggcc ccaaagagaa tggcatccac
     8041 cccttgcacg gccgctattt ccccggcctg ctccagccct tccgcggttt ctatctgggc
     8101 gatcagcagg gtgtcttcgt tggccgtgtg tgaataggcc cgtccgtgca tgtcgaccgg
     8161 tcggcggccc ccgtaacttc tttcgcccag cggcggaaac ttggccgcct ggacgattga
     8221 ttcggcctgg cgcgcgttgt tgaccatggg aaccatgatt ccggaggtgt tggtgtccag
     8281 ggccagcccg atgaagccgt aatcatgcga gggtaccctt accaccgatg gccgccgtac
     8341 gagttcgcag gcccggacgc agccgaggat ggaattataa tcatgctctc cgtgctggcc
     8401 gtcgatccag atccaatccc aatcccttcc caccagttcg ataatgccgg gaaccgggta
     8461 acagatggat attcccagga gcactcgctt ccgggccagt tcgtccttga tccttaccat
     8521 ggccatggcc agctcccaat ccggtattat tttcggtttg ccccggcgct tgccgggagc
     8581 cgcagcgtcg ttttaatgat cgccaatgat cgcttgcctt atcaaaaaga cggaccggtt
     8641 cacttcccgg tccggccggt ttcctgctcc cggcgcgcca ggagttccgc gagccccttt
     8701 cggtaggcct cctgcttgtc aagcccgccc agcatgttat cgtccaacac cgggatcttc
     8761 tcgctgagcc ggaagaccag cgtgccgagg ttgaccaccg agccgtagtc caggcccagc
     8821 aagccgaaga ccttgttgtt gatccgttcc acctcggcga ccatgtgcgg gtcctcggcg
     8881 ctcgtgcagg tatgccgttt gccccagatg gagccggttt cgctttcccg gcggaacttg
     8941 cagctctcgc aggcatcatc gtgtccgcgc acgatctcga tttcaatttc ctccagcggg
     9001 ttctcccgga ttttgcgctg gatggactcg aagccggccc gggcgcggct ctcctggggt
     9061 atgccgcccc cataatgggc cacgataaaa gtcggatgat acggtctcag tcgaatacgt
     9121 actggcatgg cacctccttt ttgcctgagc ggaatccgcg gaaagaataa tatcagccgc
     9181 cgtttttgtc aaatgcggag ccccggcggt ccaggggacg cctcctttcc ggccgggcgg
     9241 cgcttccgga attcttgaca aaaaggtgcc gttcattaat aatgaaaaag aagtttgccg
     9301 gtggctggat cttcattctt aaagggtttc ccgggtggaa ccagggtcga aaacaggtga
     9361 aaaaggcggt cttgcttcta tttctgctgg tcctgttcgc cggcctcggg cgggcggccg
     9421 ccggtattga acccggtccg gaagtggtcc tctgggcctg ggaacggccc gaaaacctgc
     9481 tctttctcaa ggacggccgg gtccgggtgg ccttttacgc cgggagcgtc accttcgccg
     9541 gctcgcagac ccttttcctg ccgcgccgcc agccgttgct ggttttaccg gccgtccccc
     9601 tgacggccgt cttccggatc gagaaccgcc aggcggccgc gcccggcggc cgccagctct
     9661 ccgagagcat cgaggtcatt gcccggtctt ttgccggccg gggcataacc ggcctccagc
     9721 tcgacttcga ctgccgggcc tcggagcggc ccttcctgga gggattggtg gaagggttga
     9781 agcggcgcct cccgccctcg accgccctct ccgtgaccgt gctggcctcc ctgagccacg
     9841 cgggcagctg gatcgacacc ctgcccgtgg acgaggccgt cccgatgctc ttccggatgg
     9901 gccctgacga ttacctggtg cggaagcagc tggccggccg ctccttcctg aaggccgccg
     9961 cctgccggaa ggcggtcggc ctctccctcg acgagcccct tccgccggcc gcttacctga
    10021 aggagcgccg gatctacctt ttcaacccgc atccctggac cggcgaggcc cttgaccggg
    10081 cccggctgga aatcaggcgc cgcctggagg aggataattc cccatgaaag gttccggccc
    10141 gaagttccgt ttcctgctct tctttccgct cctcctgctg gtggccgccc cggcctcggc
    10201 ctgcggtccc ttcttccccc attacctcta tacgcccgag gatatcgccg aggtcggctc
    10261 cccttactcc tacaaccgca acttttccgg cgcctcgccg gggatcatct ttccggcctg
    10321 gcggacctcc gtcctctacg aggtgtaccg gggactctcc ggccgccttc cggtcgagtc
    10381 actctccgcg gccccggtcg acgagccggc cgatccggcc ggttggctgg ccgcccggaa
    10441 gacggtcacg gagacggcgg ttgagatccg ggactgggcc gaaggggaga actactccta
    10501 ctaccagaat tacctggccg acgcctttgg caccgccgcg cgcaccctct ccgagcggaa
    10561 accagtcttc acgccggagg agttgaagct ctggctcgcg gggcaggacg aggtcttcgg
    10621 cagcgccccg gaccgcctct tcgacgcgga gctggaccgg tccatcagta acccgctcct
    10681 caggcaggac cgggagtacc agcgggcggc cgcctgcttc taccggagga aatacgacga
    10741 ggccgaagcc cgtttccggg agatcgccgg gaaccccggc cacccctgga gggtttatgc
    10801 ggccctggcc gcgggccgga ccctgattcg ccgggccggc ctggaatacg agctggaatt
    10861 caagaagtcc ggcggcggcg gggaggggga taaaaaggcg ctcgcgatcc gaaacgagtg
    10921 gctgctgaag gcccgggccc agctggaggg tattctgaag gacgcgaccc tgcgaacagt
    10981 ccatccctcg gcccggcgcc tgctcgatta tatcgtcttc cggctcgagc cgcgggagcg
    11041 cctgctggcg gccgaaaagg tcctgctcct ttcggaggat gccgctgaaa tacggatgaa
    11101 cctgcgtgat ttcgcccttc tgcatggcc
//
LOCUS       DAOQYN010000040        11053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_6356_length_11053_cov_3.995817, whole genome shotgun sequence.
ACCESSION   DAOQYN010000040 DAOQYN010000000
VERSION     DAOQYN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11053
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6356_length_11053_cov_3.995817"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="PKN80_02780"
     CDS             <1..986
                     /locus_tag="PKN80_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNQ34970.1"
                     /translation="KTDLALLKINAGGLPFAPFGDSSAQPVGEWVLAIGNPFGLEGTV
                     TVGVVSAKGRHLGINPIESFIQTDASINPGNSGGPLVNLKGEVIGINTAILSGGQGLG
                     FAIPSNLAREVFSQLRTQGKVVRGWLGVSIQPIGPELSKELNLGGTGGVLLSNVFPKQ
                     PAERAGLKAGDVIVAVNNRSVASPEELQQLLSELKPGQVVTVTAVRDGKNQVFRVRLG
                     EQPAELISEEPAGPEEAGSWRGLKLTTLTPEVAQSAGLSLTSGVLVVDVEGRGPLAGK
                     IAPGTVIVEARRQAVNNLSEFLKAVDIPDNQAVLLRIFTRNNFQYLVVPGQ"
     gene            complement(1030..1545)
                     /locus_tag="PKN80_02785"
     CDS             complement(1030..1545)
                     /locus_tag="PKN80_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNQ34971.1"
                     /translation="MALHNQEATILVGERYPILESTITGTPPNQYITESLSHYEPIGV
                     TLRVIPRVVDGGRINLLLHPEISSLGPDVEGVSGVVYKRINTREADTNVSVTNGQTMV
                     IGGLLTNVKEDNVYKVPFLGDIPLLGNLFKRVEKRPAQRELLIFITPRLMVDDIGDDS
                     ALMQDKTSQFR"
     gene            complement(1437..2474)
                     /locus_tag="PKN80_02790"
     CDS             complement(1437..2474)
                     /locus_tag="PKN80_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HNQ34972.1"
                     /translation="MKKTMVWIRGILIFGLLFLSVAAGTAGADQVTPGEGKLAARRIV
                     RLELRNAELADVLRLFSKEYALNIVAGRDISGTVTISLNQVDAADALRTILVANGYDY
                     EQVGDIYMVTTRENILARRKNLLEGEALETRIFWLNYLGAQDLARVIEKELSPRGNLR
                     FLSQTILNGWEMGGVSGSETSGELGRKIRLKKEQDEKAPVLVVSDIPAVLNRIGALIR
                     ELDVKPEQVLIEAVIVEVNSEMVRDLGIEWKYTHTDGDITSGFIPGTVTPPAPGFTGL
                     EVAYDRAVGENPLMVTLRALEEKKKANISPGPGSWPCTTRRPPSWSGNATRSWNRPSP
                     GLHPTSTLPRA"
     gene            complement(2575..3117)
                     /locus_tag="PKN80_02795"
     CDS             complement(2575..3117)
                     /locus_tag="PKN80_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34973.1"
                     /translation="MNNQKKYLLMGVMLVCSIIIFAAGLRFLGPRTPRVVDTKTGSPG
                     VAGTDLDTAGPAGTDDFWAEAELLLNEAPLFIQPVGDAYVPPRRSPIFKKGPGVSIHS
                     ILAGVQSPEAGSPDGAAGLPRAIAVLNGIMVVDGRSTAIVNGEIVRAGDRVAGAEVVE
                     IKADQVKLRKGQEEHFLVLE"
     gene            complement(3129..3722)
                     /locus_tag="PKN80_02800"
     CDS             complement(3129..3722)
                     /locus_tag="PKN80_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34974.1"
                     /translation="MISRKLIGGLVLAALLCFFFYLVVHSMVLAPQFREIKSLKGQLL
                     SMESLPAVDAASASFLRLAASFRSAAARLDQCLLEGDGLPVLTLSRLAGRSGVSLIAV
                     EPAAGQAGTDSGLFNPVDFQVSFSGSYRATADYIHALEHDAIFFQVRQLAAGLSDTAD
                     QTEILLRCYTRREGAPGSAGGSGPAAVRKAGAPEKGL"
     gene            complement(3719..4267)
                     /locus_tag="PKN80_02805"
     CDS             complement(3719..4267)
                     /locus_tag="PKN80_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34975.1"
                     /translation="MVEINLIRKRILRPASERRLVSILVYLVLGMVGLTLLAIFIPYA
                     ANLKIIEGYRQKIRVAEAEWETNFRPQPAAGAPERRQLEELKNFKPLFQGRLLMAPKL
                     SALAAQAEPGLVLSGVRFDGTKMALEGEGRRDNQTMVAVVRLVNRLNATPAFREGFQE
                     LRIKWIRQDQDRFRFLVEASRQ"
     gene            complement(4307..5263)
                     /gene="pilM"
                     /locus_tag="PKN80_02810"
     CDS             complement(4307..5263)
                     /gene="pilM"
                     /locus_tag="PKN80_02810"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNQ34976.1"
                     /translation="MASNGFQVSLDVGSRFLKLLVRDGRGQTGFEMAPRPVDEKELAA
                     LLRGWDEKYRLAGRPLVSSLSGPEINIQYLNFPALPAAELESAVNLEAGQILGIENLA
                     AMDTDFWPLEASGNRSQVLFVACPQKLSNERLKLCHRSGLKLEKLTIDCLALANGYLN
                     LETGGPATMLLNLGSRLTNTAVVEGGRLLLGRDILWGGERVAQGISEGLDQNLPRAIK
                     ILEEDSGRVRERLPEILDRTASGLIEEIEKTVAYCEGTHKVKAARLLLTGGASLIPGL
                     NDFISERVSLPVQEWNAAKYFPADSILKSQVHFAAVAVGMLF"
     gene            complement(5325..5753)
                     /locus_tag="PKN80_02815"
     CDS             complement(5325..5753)
                     /locus_tag="PKN80_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ34977.1"
                     /translation="MVLNLKGRQGFTMIELMIVVIIVGILASAAVPLYRGNIRRAIKA
                     EAEATLGSIRTAERTYKAEHDTYTDATSAEVASLLGIQVIDPQYLDADCYQVVNATAT
                     AFEAQCVVNNTDAAPGAATAKRYLGTGTVVSMDQNGTLSE"
     gene            complement(5823..6251)
                     /locus_tag="PKN80_02820"
     CDS             complement(5823..6251)
                     /locus_tag="PKN80_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ34978.1"
                     /translation="MKKNCRNQKGFTMIELMIVVIIVAILAAAAVPLYRANIRRAIKA
                     EAEATLGSIRTAERTYKAEHDTYTDATSAEVASLLGIQVIDPQYLDADCYQVVNATAT
                     AFEAQCVVNNTDAAPGAATAKRYLGAGTVVSMDQNGTLSE"
     gene            complement(6334..7545)
                     /locus_tag="PKN80_02825"
     CDS             complement(6334..7545)
                     /locus_tag="PKN80_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ34979.1"
                     /translation="MARFVYYARDPAGRPVRAATEAESVAELMARLKERNLTVVSARE
                     VRSRESSRGVRNVSTIRGTEISFFFRQLATMVGAGVSLTDALRELAVQTGNQSLRGLI
                     TGIRVEIENGINFSQALARYPHYFPPLVTAMVQSGEEGGRISEVLEQISVYLEDKLAL
                     QREVKTASTYPVFIGVFFLFSLAFITFFLIPKFEEIFKGFRVALPPLTRFVVGSSHFL
                     LKNLPFVILFLVAAGLILYRYGRTRPGRRLFDILKVRLPVIGEIFHMVSISYFCQTFA
                     VLLSSGVSVIRCLEIVGKVAGNSIIEESSEAVRLGVERGGNLSSEMRLHPIFPALLVR
                     MIAVGEQTGSLGEMFIRVNKFYRDEAMLKTRVLTAVIEPVMLLGLGVIVGVVVIALYL
                     PIFKMTGSVGV"
     gene            complement(7550..8617)
                     /locus_tag="PKN80_02830"
     CDS             complement(7550..8617)
                     /locus_tag="PKN80_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNQ34980.1"
                     /translation="MIVTIDTLLKLTVEKGASDLHLSAGTPPCLRIDGELQFCDLPPL
                     TAELCHELCYSLLEHEQKRRFETFKELDFSHGVSGLGRFRVNYYYQRGSIGAAFRPIL
                     TEVPGFDVLGLPQAAVRKLASMPKGFVLVVGPTGSGKSTTLASMINYINHTQRSHIIT
                     IEDPIEYLFRHHTSVIHQREMGGDTLSFAESLKHVLRQDPDVVLVGEMRDLETVVAAL
                     NIAETGHLVFSTLHCGEAVEAISRVIDIFPIGQQQQVKVQLSLTLSGVIAQQLLPRAS
                     GKGRVLATEVMLVNTAIKNLIREGRPQEIYSHLQMGSKHGMSTMNQSLYRLYRQKEIS
                     LETALNASSNQKELETLIARG"
     gene            complement(8614..10290)
                     /locus_tag="PKN80_02835"
     CDS             complement(8614..10290)
                     /locus_tag="PKN80_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNQ34981.1"
                     /translation="MAAMKTHKEKLGEMLLRLQLIRPEDLAAALEEQQKSRRLLGAVL
                     VEKGLVSSARIAELLKLQRSSEAVTLSLLDPAPATVKLIPERLAKRFLALAFDKGDGN
                     ELKVAMAEPSDIIAVDTIRSITGFEIHPVRARAEEILTAIQKYYYGFSSGTEASVEVP
                     EVEFAGGHEEEAETSEIKFQANDPPVIKYVNMLFVQVIENRASDIHLEPMEKSISCRF
                     RIDGVLRPGTPPATGMYPGVVSRIKIISKLDIAERRKPQDGRCEIKVGEKLLDLRISI
                     FPTVFGEKVVIRVLDRSSLILNLEELGMEGSQMDAFKNALKKPHGIILITGPTGAGKT
                     TTLYAGLAYTNNPDKNIITLEDPVEYRLEGINQTQVRPEIGLTFATYLRHVLRQDPDV
                     IMVGEIRDLETAEIAIRAALTGHLVLSTVHTNDSVSTITRLMDMGIQPYLLPPSLNLV
                     IAQRLVRRLCPECKQPGPPSSLLGPGVQCYQARGCPFCNQTGYWGRIGIYEVLEITPE
                     IRELIIKGGNPDLLKSLARKQGMKGLLPGGLDKVSQGITSLEEVLGMMED"
     gene            complement(10291..>11053)
                     /locus_tag="PKN80_02840"
     CDS             complement(10291..>11053)
                     /locus_tag="PKN80_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34982.1"
                     /translation="NAEVLGDADVPSTGSITGETYITGQKDYDADVKTPNPVVIPPAL
                     IALPYPVTGDPRISEATVAAGVLTVANNKTATLAAGNYHFKGINVRNNAILRMEGSVI
                     IYVEQDITLNQGASLRLGQPTYSMVFYVGPDGIIDLEQNSAVNIPLTTRPTQARFYVA
                     GTGTEIITSVLSAQVAIFNGLIYAPERRATIEENVDFYGGLVVRELSIDNNASIHYDA
                     SLREPPGFPEDPGTVIPAGLKLLNWTKPGWRERLL"
BASE COUNT         2070 a         3506 c         3338 g         2139 t
ORIGIN      
        1 ccaagaccga cctggccctg ctcaagatca acgccggcgg acttcccttc gccccctttg
       61 gagattcctc cgcccagccg gtgggagagt gggtgctggc catcggcaat cccttcggac
      121 tggagggcac ggtcaccgtg ggcgtggtca gcgccaaggg gcgtcacctc gggatcaacc
      181 cgatcgaaag tttcatccag accgatgcct cgatcaaccc cggcaacagc ggcggcccgc
      241 tggtcaacct gaagggcgag gtgatcggca tcaataccgc cattctttcc ggcggccagg
      301 gcctgggctt cgccattcca tccaacctgg cccgggaagt cttcagccag ctgcgcaccc
      361 agggcaaggt ggtccgcggc tggctggggg tctccatcca gccgatcgga cccgagcttt
      421 cgaaagaact caacctgggc ggaaccgggg gggtgcttct gagcaatgtc ttcccaaaac
      481 agccggccga acgggccgga ctgaaggccg gcgacgtgat cgtcgccgtc aacaaccggt
      541 cggttgcctc gccggaagaa ctccagcagc tgctgtccga gttgaaaccg gggcaggtgg
      601 ttaccgtcac cgccgttcgg gacggcaaga accaggtctt ccgggtccgg ctcggcgagc
      661 agccggccga actgatctcc gaagagccgg ccgggccgga agaggcggga agctggcgcg
      721 gcttgaaatt aacaaccctc acgccggaag tggcccaatc ggccggatta tccttgacga
      781 gcggagtcct ggtggtggat gtggagggac ggggtccgct ggccggaaag atcgccccgg
      841 gaacggtcat cgtcgaggcg cggcggcagg cggtcaacaa tctctcggaa ttcctgaagg
      901 cggttgatat tcccgacaac caggcggtcc tgttgcggat cttcacccgg aacaacttcc
      961 agtacctggt tgtcccgggt caatagaccg ccgcgccaat cgtttcgcgc ggcggccggc
     1021 cggtaacggt caacggaact ggctggtctt atcctgcatc agcgccgaat catccccgat
     1081 gtcgtcaacc atcagccggg gcgtgatgaa gatcagcaat tcacgctggg ccggccgttt
     1141 ttccacccgc ttgaaaaggt tccccagcag cggtatgtca cccaggaagg gaaccttgta
     1201 aacattgtct tccttgacgt tggtcagcag ccccccgatt accatggttt gaccgttggt
     1261 gacgctgacg ttggtgtccg cctcccgggt gttgatccgc ttgtaaacga ctccgctgac
     1321 gccctccacg tccggcccga gtgaactgat ctccgggtgc agcagaaggt tgatgcggcc
     1381 gccgtcgacc acgcgcggaa tcacccggag cgtgacgccg atcggttcgt agtgactcag
     1441 gctctcggta atgtactggt tgggtggagt cccggtgatg gtcgattcca ggatcgggta
     1501 gcgttccccg accaggatgg tggcctcctg gttgtgcagg gccatgatcc ggggccggga
     1561 gagatattgg ccttcttctt ctcctccagg gcgcgcaggg tcaccatgag cgggttttcg
     1621 ccaacggccc ggtcataagc cacttcgagc ccggtaaagc cgggcgccgg cggcgtcacc
     1681 gttcccggta tgaaaccgga ggttatgtcc ccgtcggtat gggtatattt ccattcgata
     1741 ccaagatccc ggaccatctc cgaattcacc tcgacaatca ccgcctcgat cagcacctgc
     1801 tcgggcttga cgtccagttc ccggatcagg gcgccgatcc ggttcagcac cgccgggatg
     1861 tcgctgacca ccaggaccgg ggccttctca tcctgttcct tcttaaggcg gatcttgcgc
     1921 cccagctctc ccgaagtttc ggagcccgag acgccgccca tctcccagcc gttcaggatc
     1981 gtctggctca ggaagcggag gttgccgcgc ggagaaagct ccttttcgat cacccgggcc
     2041 aggtcctggg cgcccaggta attcagccag aagatccggg tctccagggc ttccccttcc
     2101 agcaggtttt tgcgccgggc caggatattc tcccgggtgg tcaccatgta gatgtcgccg
     2161 acctgttcat aatcgtaccc gttggcgacc aggatggtgc gaagcgcgtc agcggcgtca
     2221 acctggttga ggctgatggt aaccgtgccg gaaatgtcgc gcccggccac gatattcaag
     2281 gcgtactcct tggaaaagag gcgcagcacg tcggccagtt cggcattcct caattccagg
     2341 cggacgatcc gccgggcggc cagtttccct tcacccgggg tgacctggtc cgccccggcg
     2401 gtgccggcgg ccacgcttaa gaaaagcagc ccgaagatca atatccccct tatccacacc
     2461 atggtctttt tcataaatcg atcctctatg aacgcgattg ccggagttgc gcgcctctcc
     2521 ggtcctccga ctttttctcg cctcgcaatc attgcccggt gcccttccga tccgctattc
     2581 caggaccagg aaatgctcct cctgcccctt gcggagtttt acctggtccg ccttgatttc
     2641 aaccacttcc gccccggcca cccggtctcc ggccctgact atctcgccgt tcacgatggc
     2701 cgtcgaacgc ccatccacca ccatgatccc attcaagacg gcgattgccc gcggcagacc
     2761 ggcggcgcca tccgggctcc cggcctccgg ggactgaaca ccggccagga tggaatgaat
     2821 cgacaccccc gggcccttct tgaaaatcgg gctgcggcgc ggggggacgt aagcgtcgcc
     2881 aaccggctgg atgaacagcg gcgcctcgtt gagcaggagt tcggcctccg cccagaaatc
     2941 atccgtcccg gccggaccgg cggtatccag gtccgtcccg gccacgccgg ggctgccggt
     3001 tttggtgtca accacccggg gtgtccgggg ccccaggaat ctcaagccgg ccgcgaaaat
     3061 aatgattgag cagaccagca tgactcccat cagcaggtac tttttctggt tattcataat
     3121 ggaatccgtc acaggccctt ttccggcgcc ccggccttgc ggacggccgc cgggccggag
     3181 ccgccggcac tccccggtgc cccctctctc ctggtgtaac agcgtaaaag aatttccgtc
     3241 tggtccgcgg tatcggaaag gcctgcggcc agctgccgga cctggaagaa aatggcgtcg
     3301 tgctccagcg cgtggatgta atcggccgtg gcccggtagg aaccggaaaa ggaaacctgg
     3361 aaatcaaccg ggttgaagag accgctgtcg gtgccggcct ggccggcggc cggttccacg
     3421 gcaatcaggc tgaccccgct ccggccggcc agccggctca aggtcaatac cggcaggccg
     3481 tctccttcga gcaggcactg atcgagccgg gcggcggccg aacggaacga agcggcaagc
     3541 ctgagaaagg aggccgaggc cgcgtccacg gccggcaggg attccatgga aagcagctgc
     3601 cccttaaggc ttttgatctc ccggaactgg ggcgccagca ccatggaatg aacgaccagg
     3661 tagaagaaga aacagagcag ggccgccagc accaggccgc cgattaattt tctggaaatc
     3721 attgacggga cgcctccacc aggaaccgga accggtcctg gtcctgacga atccacttga
     3781 tccggagttc ctggaaaccc tcccggaagg ccggcgtggc attgagccgg ttgaccagcc
     3841 ttaccaccgc gaccatcgtc tggttatcgc gccgcccctc tccctccagg gccattttgg
     3901 ttccgtcgaa cctgacgccg ctcagaacca ggccgggctc cgcctgggcg gccagggccg
     3961 agagtttcgg agccatcagc aggcgtccct gaaagagggg cttgaagttc ttcaactcct
     4021 ccagctggcg gcgctccgga gcaccggccg ccggctgggg acggaaattc gtttcccact
     4081 ccgcctcggc gaccctgatc ttctggcgat aaccttcgat gatcttcagg ttggccgcgt
     4141 aggggatgaa aatggccagc agggtcaaac cgaccattcc cagcaccagg taaacaagaa
     4201 tcgagacgag tctccgctcc gaggccggcc gcagaatccg ttttcgaatt aagttgatct
     4261 cgaccatcgt tctttatttc accttttttc gccggggacg gctgcttcag aagagcatcc
     4321 ccacggccac cgcggcgaag tgcacctgcg atttaagtat tgaatccgcc ggaaaatatt
     4381 tcgccgcgtt ccattcctgg accggcagcg aaacccgttc ggagataaaa tcattcaaac
     4441 ccggaatcag ggaggcgccc cccgtcaaaa gcagcctggc cgccttcacc ttgtgggtcc
     4501 cctcgcagta agccacggtc ttttcaatct cttcgatcaa cccggaagcg gtccggtcca
     4561 ggatctccgg caaacgctcc cgaacccttc ccgaatcctc ctccaggatc ttgatggccc
     4621 ggggcaggtt ctggtccagc ccctcggaga tcccctgggc aaccctttct ccgccccaga
     4681 ggatgtcccg gcccagcaga agccgccctc cctccaccac tgcggtgttg gtcaggcggc
     4741 ttcccaggtt caacagcatg gtggccgggc ccccggtctc caggttgaga taaccgttgg
     4801 ccagggccag gcaatcgatg gtgagcttct ccagtttcaa ccccgaccga tggcagagtt
     4861 tcaacctttc attgctcagt ttctggggac aggcgacgaa caaaacctgg gaccggttgc
     4921 cggacgcctc cagcggccag aagtcggtgt ccatggcggc caggttttca atgcccagta
     4981 tctggccggc ctcaagattg acggccgatt ccaattcggc ggccggaagc gccggaaaat
     5041 tcaggtactg gatattgatc tccggaccgg acaacgaaga gaccagcggc cgcccggcca
     5101 ggcggtattt ctcatcccac cctctcagca gcgccgccag ctccttctca tcaaccggac
     5161 ggggggccat ttcaaaaccg gtctggccgc gtccgtcccg gaccaggagc ttcaaaaaac
     5221 ggctgccgac atccagcgaa acctggaaac cgtttgaagc caagttaatc tcccctcttc
     5281 aattgagaaa ataccatcca gtcccgatat caacaggctt gttgttattc gctgagcgtg
     5341 ccgttctggt ccatcgagac gacggtcccg gttcccaggt aacgcttggc ggtggccgcc
     5401 ccgggcgccg cgtcggtatt attcaccacg cactgggcct cgaaggcagt cgccgtggcg
     5461 ttgaccacct ggtagcagtc ggcgtccagg tactgcggat caatcacctg gatacccagc
     5521 aggctggcca cttcggccga ggtggcatcg gtgtaggtgt cgtgttcggc cttgtaagtc
     5581 cgctcggcgg tccggatgga gcccagcgtg gcctccgcct cggccttgat ggcccgccgg
     5641 atgttacccc ggtaaagggg aacggccgcc gaagccagga tgccgacgat gatcaccacg
     5701 atcatcagct caatcatcgt aaaaccctgc cggcccttca agttcaatac catcactttc
     5761 gttgctcctt tctcgatacc ggagaggccg ccggaaccgg cggcctctca cctgtttcgg
     5821 atttactcgc tgagggtgcc gttctggtcc atggaaacga cggtcccggc ccccaggtaa
     5881 cgcttggcgg tggccgcccc gggcgccgcg tcggtattat tcaccacgca ctgggcctcg
     5941 aaggcagtcg ccgtggcgtt gaccacctgg tagcagtcgg cgtccaggta ctgcggatca
     6001 atcacctgga tacccagcag gctggccact tcggccgagg tggcatcggt gtaggtgtcg
     6061 tgttcggcct tgtaagtccg ctcggcggtc cggatggagc ccagcgtggc ctccgcctcg
     6121 gccttgatgg cccgccggat gttggcccgg taaaggggaa cggccgcggc cgcaaggatg
     6181 gctacgatga tcaccacgat catcagctcg atcatcgtaa aacccttttg gtttcggcaa
     6241 ttcttcttca tcaaatttca ccccctttcg caatcgacaa attgtttact tgactttatt
     6301 ataacacaaa atctataaaa taaaaggcct ggctcagacg cccaccgaac cggtcatttt
     6361 gaagatgggc aggtagaggg cgatgaccac cacacccacg atcacaccca ggcccagcag
     6421 catgaccggt tcgatcaccg cggtcagcac ccgggtcttg agcatcgcct cgtcccggta
     6481 gaacttgttg acccggatga acatctcccc cagcgaaccc gtctgctctc ccaccgcgat
     6541 catccttacc agcagggccg ggaagatcgg atgcaggcgc atttcactgg aaaggttgcc
     6601 gcccctttcc accccaagcc ggaccgcctc gctggactcc tcgataatgc tgttgccggc
     6661 cacctttccc acgatctcca ggcagcggat gacgctgaca ccgctggaga gcaggaccgc
     6721 gaaggtctga cagaagtagg agatggagac catgtgaaaa atttccccga ttaccggcag
     6781 ccggaccttc agaatatcga acagacgccg ccccggacgg gtgcgtccat accggtaaag
     6841 gataagcccg gccgccacca ggaaaaggat gacgaaaggc aggttcttga gcaggaaatg
     6901 gctgctcccg accacgaacc tggtcagcgg aggaagcgcc acccggaaac ctttgaagat
     6961 ctcctcgaac ttcggaataa ggaagaaggt tatgaaggcg agcgaaaaga gaaagaagac
     7021 cccgatgaaa accggatagg tggaggcggt tttaacctcg cgctggagcg ccagcttatc
     7081 ctccaggtaa accgaaatct gttcgagcac ctccgaaatt cgcccccctt cctccccgga
     7141 ttggaccatg gccgtcacca ggggcgggaa gtagtgcggg tacctggcca gcgcctgtga
     7201 gaagttgatg ccgttctcga tctccacccg gataccggta atgagccccc gcaaggactg
     7261 gttcccggtc tgcacggcca gttcccggag cgcgtcggtc agcgagacac cggccccgac
     7321 catggtggcc agctggcgga agaagaagga gatttcggtc ccccggatgg tgctgacgtt
     7381 cctcaccccc ctcgaactct cccgggacct gacctcccgg gccgagacaa cggtaaggtt
     7441 cctttccttg agcctggcca tcagttcggc cacgctctcg gcttcggtgg cggcgcggac
     7501 cggccggccg gccgggtcgc gggcgtaata gacgaatctg gccatgtttt taccccctgg
     7561 cgatcagggt ttccaattct ttctggttgg aactggcatt gagcgccgtt tccagtgaaa
     7621 tttccttctg ccggtaaaga cggtagagtg actggttcat ggtcgacatg ccgtgcttgc
     7681 tccccatctg gaggtgtgag tagatctcct gcggtcggcc ttcccggatc aggttcttga
     7741 tggcggtatt aaccagcatc acctcggtcg ccagcacccg gcctttgccg ctggccctgg
     7801 gaagcagctg ttgggcgatc accccggaaa gtgtcagcga aagctggacc ttgacctgct
     7861 gctgctggcc gatcggaaag atgtcgatga cccggctgat ggcctcgacc gcctcaccgc
     7921 agtgcagggt ggagaagacc aggtggccgg tctcggcgat attcagggcc gccaccaccg
     7981 tctccaggtc gcgcatctcg ccgaccagga ccacgtccgg gtcctgcctt aggacgtgct
     8041 tgagcgactc ggcgaaggaa agcgtgtccc cgcccatttc ccgctgatgg ataacggaag
     8101 tgtggtgtcg gaaaaggtac tcgatcggat cctcgatggt gatgatgtgc gaccgctggg
     8161 tatggttgat gtaatttatc atcgaggcca gggtggtcga cttgccgctg ccggtgggcc
     8221 ccaccaccag cacgaaaccc ttcggcatcg aggcgagctt gcggaccgcc gcctggggca
     8281 ggcccaggac atcaaaaccc ggcacctcgg tcaggatcgg ccggaaagcg gccccgatcg
     8341 aaccgcgctg gtaatagtaa ttgacccgga aacggcccaa ccccgaaacc ccatgggaaa
     8401 aatcgagttc cttgaaggtt tcgaaacggc gtttctgctc gtgttccagc agtgaatagc
     8461 atagttcatg gcacaactcc gccgtgagag gcggcaggtc gcagaactgc agttcgccgt
     8521 caatgcgaag gcagggcggc gttccggccg aaaggtgcag gtcggaggcg cctttttcca
     8581 ccgtgagctt taagagcgtg tcgattgtca cgatcaatcc tccatcattc caagaacctc
     8641 ctccagggaa gtgattccct gggagacctt gtccagcccg cccggcagca gtcccttcat
     8701 accctgctta cgtgccaggc tcttgagaag atcggggttg ccccccttga tgatcagctc
     8761 gcgtatttcc ggggtgatct ccagcacctc gtagatcccg atccgccccc agtaaccggt
     8821 ttggttgcag aaggggcagc cgcgtgcctg gtagcactgg accccggggc cgagcaggct
     8881 cgagggcggc cccggctgct tgcattccgg gcagaggcga cgcaccaggc gctgggcgat
     8941 gaccaggttg agcgaaggcg gcagcaggta gggctggatc cccatgtcca tcaggcgggt
     9001 gatcgtggag acgctgtcgt tggtgtgcac cgtggaaaga accaggtggc cggtcagggc
     9061 cgcccggatg gcgatttcgg ccgtctccag gtcgcggatc tcgccgacca tgatcacgtc
     9121 cgggtcctgg cgcagcacgt ggcgcaggta agtggcgaag gtcaacccga tctccggccg
     9181 gacctgggtc tggttgatcc cctcgagccg gtattcaacc gggtcctcca gggtgatgat
     9241 gttcttgtcc ggattgttgg tataggctaa cccggcatag agggtggtgg tcttgccggc
     9301 gccggtcgga ccggtgatga ggatgatccc gtgcggcttc ttcaaggcat tcttgaaggc
     9361 atccatctgg gacccttcca tgcccagttc ctcgaggttc aggatcaggc tgctccggtc
     9421 caggacccgg atgaccacct tctctccgaa gacggtcgga aagattgaga tgcgcaggtc
     9481 cagtagtttc tcgcccacct tgatttcgca gcggccgtcc tggggcttgc gccgttcggc
     9541 gatgtccagc ttggagatga tcttgatccg gctgaccacc cccgggtaca tgccggtcgc
     9601 cggcggggtg cccggcctca gcaccccgtc aatccggaat cggcacgaga tcgacttctc
     9661 catcggctcc aggtggatat ccgaggcccg gttttcgata acctggacaa agagcatgtt
     9721 gacatacttg atgaccggcg gatcgttcgc ctggaacttg atctcggagg tctcggcttc
     9781 ctcctcatgg ccgccggcga attcgacctc cggcacctcg acggacgcct cggtcccgga
     9841 actgaacccg tagtaatact tctggatggc agtcaggatc tcctcggccc gggcccgaac
     9901 cgggtggatc tcgaagccgg tgatcgagcg gatcgtgtcc acggcgatga tgtcgctcgg
     9961 ttcggccatg gccaccttca actcgttccc gtcgcccttg tcgaaggcca gcgccaggaa
    10021 ccgcttggcc agccgctccg ggatcagctt caccgtggcc ggcgccgggt ccagcaggga
    10081 aagggtcacc gcttcggaac tccgctggag cttgagcaat tcggctatcc tggcgctgct
    10141 gaccaggccc ttttcaacca ggaccgcgcc cagcagccgc cgggacttct gctgctcctc
    10201 gagggccgcg gccaggtctt ccggccggat cagctgcagg cgcagcagca tctcgcccag
    10261 tttctccttg tgggtcttca tcgcggccat ctaaagcagc ctttcccgcc agccgggctt
    10321 ggtccagttg agcagtttca acccggccgg gatgaccgtc cccgggtcct ccggaaaacc
    10381 gggcggctcg cgcaggctgg cgtcataatg gatggaggcg ttgttgtcga tgctcagttc
    10441 ccggaccacc aggccgccgt aaaagtcaac attctcctcg atggtcgccc ggcgctcggg
    10501 ggcgtagatc aagccgttga agatggccac ctgggcgctg aggactgacg tgataatctc
    10561 ggtgccggtc ccggccacgt agaaacgggc ctgagtcggc cgggtggtca acgggatgtt
    10621 gacggccgag ttctgctcga ggtcgatgat gccgtccgga ccgacataga agaccatcga
    10681 gtaagtcggc tggcccaagc gcaggcttgc cccctggttc agggtgatgt cctgctccac
    10741 gtaaatgata acgcttcctt ccatccgtaa aatggcatta ttacggacat taatcccctt
    10801 gaaatgatag ttcccggccg ccagggtggc ggttttgttg ttggccacgg tcaggacccc
    10861 ggcggccacg gtggcttcgc tgatgcgcgg atcgccggtg accgggtagg gcagggcgat
    10921 cagcgccggg gggatgacca ccgggttggg ggtcttgacg tcggcgtcat aatccttctg
    10981 gccggtgata taggtttcgc cggtaatgct tccggtcgac gggacatcgg cgtctccgag
    11041 aacctcggcg tta
//
LOCUS       DAOQYN010000041        10895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_6467_length_10895_cov_4.930166, whole genome shotgun sequence.
ACCESSION   DAOQYN010000041 DAOQYN010000000
VERSION     DAOQYN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10895
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6467_length_10895_cov_4.930166"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PKN80_02845"
     CDS             <1..568
                     /locus_tag="PKN80_02845"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNQ34983.1"
                     /translation="VAAEGLLEKFRSVTGRPLLLTIRSDRERETVLEPPLSEPDRLSL
                     FARLLPRCDAVDVEIGAGIAGRVARLAREAGKPVIGSYHNFRRLPPFFRLEEKLNRAE
                     QLGAAVFKAAVRAEKTGEVFQLLDFCRRASGPHLVAAVPVGGRSPLPRLLAGYFGSVL
                     VYAALGRPTAAGQPGLAELAEDLKRFYR"
     gene            616..1398
                     /locus_tag="PKN80_02850"
     CDS             616..1398
                     /locus_tag="PKN80_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNQ34984.1"
                     /translation="MILPALTSVTFRKLAPEEVVALAAGSGLKGIEWGGDIHVPPGRP
                     EMARRVRALTRSAGLEVSSYGSYYRAGHPETGPFEAVLETALELEAPAIRIWAGRRAS
                     AEADPAYRAAVTADVVRVCRLAAAAGRTVAFEYHANTLTDESLSARRLLLEADQSNLR
                     TYWQPPNGLGLGESLQSLKAILPWLSNLHVFYWNPDGKSRRPLAEGTGLWRRYLSVAY
                     AVPGRHYAILEFVMDDAPENFRLDALTLLGWLEELRPPEYQI"
     gene            1427..2524
                     /locus_tag="PKN80_02855"
     CDS             1427..2524
                     /locus_tag="PKN80_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ34985.1"
                     /translation="MAKTMKAGVVGAGGISGYHIGGLRAAGVEVAAIADPELARARAR
                     AGEYAVGGVFESYREMLAKCPELDLVTVGVPNKYHAEVARAALEAGKHVFCEKPPALN
                     AAETLEMKEAAERRGLVLMFDFNNRARPEAQALLDYIRRGEVGRINSAQATWVRRCGI
                     PGFGGWFTQKALSGGGPVIDLLHMIDLALYFMGFPEPEWVLAQTFDDFINDKAFKGPW
                     GIPDVAGSRSDVESAAHSFIRFKSGQVLFCRNSWAEMVGREEVSVTFQGSRAGGMIRR
                     LFKRDGLDDELNASDDCELYGMENGLRVNRRIVVDPDPKMGRERAVVNFVNTILGSEE
                     PYSKPAEAVILMRVIEAIYRSAAAGEPVRIA"
     gene            2573..5152
                     /locus_tag="PKN80_02860"
     CDS             2573..5152
                     /locus_tag="PKN80_02860"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HNQ34986.1"
                     /translation="MHKDGRPYPPKRLAGGGRRSFSGARAPQVAFPLGGIGAGGICLN
                     ASGALQDFSIHNQPTLSALPDGLAATDAGFALLHLPGRRPVTRLVEGLLPPERVYDQG
                     LKGRGLRQGGYEGLPRFEACRFRGEFPVARLRLSDPGLPLAVELTGWSPFIPLDDRSS
                     GLPCAILEYRLVNRSDRPVPFEFSYHLSHLAPGREPGSRHSRSRVLPGRGIFFYNLDP
                     ANSETFGSASLSVIGHRPLIKAEWFRGGWFDAVAVLWREVSEGAFRANQASDDPEATG
                     RNGGSIMMAGRLGPGRSVTYPILITWHFPNGHLRIGAPGGAAAPPGAAAGDPPPAWRT
                     YSAGQWPDAAAVAEHIHRDFVGLRRRTFAFRRALFASDLPGYVLDAVSANLAIMRSPT
                     VLRLENGHLWCWEGCCHTGGCCSGSCTHVWNYAQAFAHLFPALERTLRAQEYGNSMDK
                     RGHVTFRSALPEGPAGHAFHAAADGQLGGVMKVYRDWQISGDSGWLKRFYPLARRSLD
                     YCIRTWDPDRKGSLFEPHHNTYDIEFWGPDGMCTSIYLGALTALAEMAEALGRPEEAA
                     PYRELAARGGRFMDRELFNGEYYQQRVMARELRDRSFAEKVDGPGRGPLLEILRREGP
                     RYQYGQGCLADGVIGAWMARLYGLELPLEPEHVRRSLRSIFRHNFKADLKEHACTQRP
                     GYAIGHEAGLLLCTWPRGGRPTLPFPYSDEVWTGFEYQVAGHLIMEGMAAEGLTLVRA
                     ARDRYDGRTRNPWNEYECGNYYARAMSSYALLAALSGFHYSAVTGRLRLAPRLDGARF
                     RCFFSTASGYGSVSLDGRGLRIDVLEGELRVRRVELDWKGKRVSREWPVRAAPGQPAR
                     LIF"
     gene            complement(5225..5881)
                     /locus_tag="PKN80_02865"
     CDS             complement(5225..5881)
                     /locus_tag="PKN80_02865"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34987.1"
                     /translation="MELIFAPTMDMLKRFWYGLSNFVLVLVFFLIGWLIAKIITLLLI
                     HAFRTMKIDRIMEESGFKSLLEKGGVKRPASEMVANLIYWVLLLVVVFLALSIGGIAI
                     PTNIIGTLLAFIPKLLLGIVIFVFAMFVGKILRGVVKTSAANSGVDKADTLAKIAEIA
                     VLIFGAVLSMQVVGIAAEFIGTAFNIVLASICFGAALAFALGSKDLIREWLEGFKKKS
                     "
     gene            6076..6462
                     /locus_tag="PKN80_02870"
     CDS             6076..6462
                     /locus_tag="PKN80_02870"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNQ34988.1"
                     /translation="MEELKVKFRKGRSFEASIRQHRLPVDLPLGLKGSDTGPTPPDYL
                     VLSLASCVGMYVLFFCERSGLSPEGLEVQAEYAKDQERITSIAVSVRLPSAETEERRR
                     EALGWAEKCLIHRTLCERPDLKISLR"
     gene            complement(6469..7338)
                     /locus_tag="PKN80_02875"
     CDS             complement(6469..7338)
                     /locus_tag="PKN80_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34989.1"
                     /translation="MTRPEIEALILKTVDTGRYQRPLLISGGGRDDRFQLAHFFVQAL
                     FCSRRTEGRPPCNRCPQCRSIKEESHPDFKFIEGAGEQGLLKIEQVRQLKESLNLSPY
                     QAEHRVYLLEVEAIREEAANAFLKVLEEPPQSAVIVLLAPSERQFMPTVLSRLNQLRL
                     PDAEAAVPETDRMNRILSLSWSRPTEIFALAQELAGKKEDPEKMDSFLIELVNSLNLA
                     LKLKAGTALENAPAAVRELADRSRDTAELAALLDEVLRKRQDILRGRVSPRLALEFLL
                     IIISRPRRGRSSP"
     gene            complement(7360..7986)
                     /gene="tmk"
                     /locus_tag="PKN80_02880"
     CDS             complement(7360..7986)
                     /gene="tmk"
                     /locus_tag="PKN80_02880"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNQ34990.1"
                     /translation="MKRGFFITFEGTDGCGKSTQLKLAAGYLRDLGLNTLLTKDPGGT
                     PAGEAIRRVLLERRPGEPDLDSWTEAFLYLASRRSLLTSVILPARAGGRVVVCDRFTD
                     STLAYQGYGRGLPLKQLTDACRLAVDGLQPDLTILLDLDPDKALARCGQPDRLEAAGL
                     EFQRRVRNGFLTLARQEPARIRVIDASGSRPEVFRRVRNELSNLLVSD"
     gene            complement(8106..9017)
                     /locus_tag="PKN80_02885"
     CDS             complement(8106..9017)
                     /locus_tag="PKN80_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ34991.1"
                     /translation="MPFKPITRTELSRRANAARAALSACRLCPRECRIDRRAGRRGRC
                     GMDARVRVASHNLHFGEEPPISGHRGSGTIFFSGCPLSCLFCQNFPISQRREGREVEI
                     EELAAMMLELQERGAHNLNLVTPTHYVPQFLEALVQARGRGFRLPIVYNSSGYESLET
                     LAWLEGVVAVYLPDLKYGDDAAGRELSGVDDYFTRAGRAIREIFRQAGPLRTDREGVA
                     VSGVILRHLVLPDNLARSDRVLRFLARAVSTKLTVSLMSQYFPAHRAAGYPQLARRLE
                     PAEYMKLVKLAKKLGFQELFIQEITDS"
     gene            complement(9032..>10895)
                     /gene="priA"
                     /locus_tag="PKN80_02890"
     CDS             complement(9032..>10895)
                     /gene="priA"
                     /locus_tag="PKN80_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNQ34992.1"
                     /translation="IFRLAGWLADYYCAPLGEVLGMALAASLQPPKRTPAEAETEEFS
                     QGIIGGLRHWPPTEQQAAVLAELEKLAAADGKRVALLKGPAATGKTEVYLQLAGRLAE
                     AGGSQDSPSLGGPQDSPYLGGPKDSPSLGGQSLILVPEIALAPQVESWARERFGEKAA
                     VLHSRLSRSRHYRIFRDAAAGRISVVIGTRSAVFTPFARLKLIVVDEEFDRSYKETRQ
                     PRYHAREAALKRGELAGALVLLVSATPALETWRAAGAGEIRSFTLTERIGRLELPKIY
                     ISDLRAGPADGRGRRSEKGLVSPRLLSLLEERLLKGEQSIIFVNRRGYTTYLFCPNCG
                     QRFTCPTCAVNLVFHRTTGELYCHYCNYRREPPPACPACGAVNLGRRAAGTQALEREL
                     QRRLPDAAIVRLDSDTLSRSGGLAELEKFRSGRAQILIGTQMITKGHHFPGVTLAAVL
                     GADNLLNRPDFRAAERYFQLLFQLAGRAGRSLPNAEVLIQTGNPEHYALTALAAYDLE
                     GFYRQELEYRRELGYPPFGRLANLVFSGRKEETAERKARAWLELLTPAAERLGVELLG
                     PAACYHKKLRNRYRWHLLLKSGRPGDIPALLNSLPEKRLYEGGLTLDLDPVDLF"
BASE COUNT         1679 a         3641 c         3784 g         1791 t
ORIGIN      
        1 cgtggccgcc gaggggctcc tggaaaagtt ccggtcggtg accggccggc cgctgctgct
       61 gaccatccgg tccgaccggg aacgggaaac ggtcctcgag ccgcccctct ccgaaccgga
      121 ccggctctcc cttttcgccc ggctgctgcc ccgctgcgac gcggtggacg tcgagatcgg
      181 ggccgggatc gccggccggg tggcccggct ggctcgggag gccggcaagc cggtgatcgg
      241 ttcctaccac aacttccgcc ggctgccgcc cttcttccgc ctggaggaga agctgaaccg
      301 ggccgaacaa ttgggcgcgg cggtcttcaa ggcggcggtc cgggccgaaa agaccggcga
      361 ggtctttcaa ctgctcgatt tctgccggcg tgcctccggg ccgcacctgg tggccgcggt
      421 gccggtgggc ggccgcagtc cgctgccccg gttgctggcc ggttacttcg gttcggtcct
      481 ggtttacgcg gccctgggcc ggcccaccgc ggccgggcag cccggcctgg ccgaactggc
      541 cgaagacctt aaacgattct accgttagcg acggccggcc ggtagccggc gccgttgaga
      601 aaagagagga atcagatgat tctgccggcc ttgacctcgg tcacctttcg aaaactggcc
      661 ccggaagagg tggtggccct ggccgccggg tcgggattga agggtatcga atggggcggc
      721 gacatccacg tgccgccggg gcggccggag atggcccgtc gggtccgggc gctcacccgt
      781 tccgccgggc tggaggtctc ctcctacggc tcctattacc gggccggcca cccggagacc
      841 ggccccttcg aggcggtcct ggagacggcc ctggaactgg aggcaccggc catccggatc
      901 tgggccggcc gccgcgcctc ggccgaggcc gacccggcct accgggccgc ggtgaccgcg
      961 gacgtggtcc gcgtctgccg gctggccgcc gccgccggcc ggacggtcgc cttcgagtac
     1021 cacgccaaca cgcttaccga tgaaagttta tcggcgcgcc ggctgttgct ggaggccgat
     1081 cagtccaacc tgcgcactta ctggcagccg cccaacgggc tgggcctggg cgaaagcctc
     1141 cagagtttga aggcaatcct gccctggctg agcaacctgc atgtcttcta ctggaacccg
     1201 gacgggaagt cccggcgtcc gctggccgag gggaccgggc tatggcgccg gtacctttcg
     1261 gtcgcctacg cggtgcccgg ccgccattac gcgatcctgg agttcgtgat ggacgacgca
     1321 ccggagaact tccggctgga cgccctgacc ctgctgggct ggctcgagga gctccggccc
     1381 ccggagtacc agatctgaaa acataaccga attcgggagg cgggcaatgg caaaaacaat
     1441 gaaggccggc gtggtgggcg ccggcggcat cagcggttac cacatcggcg gcctgcgggc
     1501 ggccggggtc gaggtggcgg ccatcgccga cccggagttg gcccgggccc gggcccgggc
     1561 cggggagtac gccgtcgggg gcgtcttcga atcgtaccgg gagatgctcg cgaagtgccc
     1621 ggaactggac ctggtcaccg tgggcgtgcc caacaagtac cacgccgagg tcgcccgggc
     1681 cgccctcgag gccggcaagc acgtcttctg cgagaaaccg ccggccctga acgcggccga
     1741 gacgttggaa atgaaggagg cggccgagcg gcggggtctg gtgctgatgt tcgatttcaa
     1801 caaccgggcc cggcccgagg cccaggcgct gctcgattac atccgccggg gcgaggtggg
     1861 ccggatcaac tcagcccagg cgacctgggt caggcgctgc ggaatcccgg gcttcggagg
     1921 ctggtttacc cagaaggccc tctccggcgg cggcccggtc atcgacctgc tgcacatgat
     1981 cgacctggcc ctctatttca tgggctttcc ggagccggaa tgggtgctgg cccagacctt
     2041 cgacgatttc atcaacgata aggccttcaa gggtccctgg ggcatcccgg acgtggccgg
     2101 aagccggagc gatgtcgaat cggccgcgca cagcttcatc cgcttcaaga gcggccaggt
     2161 gctcttctgc cggaacagct gggcggagat ggtcggccgc gaggaagttt cggtgacctt
     2221 ccagggttcg cgggcgggcg gcatgatccg gcgcctcttc aagcgggacg gcctggacga
     2281 cgaactgaac gcctccgacg actgcgagct ctacggtatg gagaacggcc tccgggtcaa
     2341 ccgacggatc gtggtcgacc cggatccgaa gatggggcgc gaacgggcgg tggtcaattt
     2401 cgtcaacacc attctgggca gcgaggagcc ttattccaag ccggccgagg cggtcatcct
     2461 gatgcgggtg atcgaggcca tctaccggtc ggcggccgcg ggcgaaccgg tccggatcgc
     2521 ctgaggcggg cggccggcgg ccgccggttt ttcagttttg ccggaggcgg tgatgcataa
     2581 agacggtcgt ccttacccac cgaaacggct ggccggcggc ggccggcgct ccttctccgg
     2641 cgcccgggcc ccgcaggtgg cttttccgct gggcggcatc ggcgccggcg gcatctgcct
     2701 caacgccagc ggcgccctcc aggatttttc gatccataac cagccgaccc tctcggccct
     2761 gcccgacggg ctggcggcca ccgatgccgg ttttgccctg ctccacctgc cgggccggcg
     2821 gccggtaacc cggctggtcg agggactgct tcccccggag cgcgtttacg accaggggct
     2881 caaggggcgc ggcctgcgcc agggcggcta cgagggcctg ccccggttcg aggcgtgccg
     2941 gttccggggc gaattcccgg tcgcccgcct ccgcctttcg gatcccggcc tgccgctggc
     3001 ggtggaactg accggctgga gtcccttcat acccctggac gaccgcagct ccggcctccc
     3061 ctgcgctatc ctggagtacc gcctggtcaa ccgttccgac cggccggtcc ccttcgagtt
     3121 ctcctaccat ctcagccacc tggcgcccgg ccgggagccg ggatcccgcc acagccgcag
     3181 ccgggttctg cccggccgcg ggatcttctt ctacaacctc gacccggcca acagcgaaac
     3241 cttcggcagc gccagcctga gcgtgatcgg ccaccggccg ctgatcaagg ccgagtggtt
     3301 ccggggtggc tggtttgacg cggtggcggt tctctggcgg gaggtttccg agggggcctt
     3361 tcgggccaac caggcctcgg acgacccgga ggcgaccggc cgcaacggcg gttcgatcat
     3421 gatggccggc cggctcgggc cgggccggtc ggtgacctac ccgatcctga tcacctggca
     3481 ttttcccaac gggcacctgc ggatcggcgc cccgggcggg gccgcggccc cgcccggggc
     3541 ggcggccggc gatccgccgc cggcctggcg cacctactcc gcgggccagt ggccggacgc
     3601 ggcggcggtg gccgaacaca tccaccggga tttcgtcgga ctgcgccgcc ggacctttgc
     3661 cttccgtcgg gccctcttcg cctccgacct gcccggttac gtgctggacg cggtctcggc
     3721 caacctggcc atcatgcgtt cgcccaccgt gctgcggctg gagaacggcc acctctggtg
     3781 ctgggagggc tgttgccata ccggcggctg ctgctccggc tcctgcaccc atgtctggaa
     3841 ttacgcccag gccttcgccc atctcttccc ggccctcgaa cggaccctgc gcgcccagga
     3901 gtacgggaac tcgatggaca agcgggggca cgtgaccttt cgttcggccc tgccggaggg
     3961 accggccggc cacgcctttc acgcggccgc cgacgggcag ctgggcggag tcatgaaggt
     4021 ttaccgagac tggcagatca gcggcgactc cggctggctg aagcgtttct acccgctggc
     4081 ccggcgcagc ctcgattact gcattcggac ctgggacccg gaccggaagg ggtccctctt
     4141 cgaaccccac cacaacacct acgacatcga gttctggggg ccggacggca tgtgcaccag
     4201 catctacctg ggtgcgctga ccgccctggc cgaaatggcc gaggcgctcg gccggccgga
     4261 ggaggcggcc ccttaccggg agctggccgc ccggggcggc cgcttcatgg accgggagct
     4321 tttcaacgga gagtactacc agcagcgggt gatggcccgc gaactgcgcg atcgctcatt
     4381 cgcggaaaaa gttgacggac cgggccgggg gccgctcctg gaaattctgc ggcgcgaggg
     4441 gccccgttac cagtatggcc agggctgcct ggccgacgga gtgatcggcg cctggatggc
     4501 ccgcctttac ggcctggaac tgccgctgga acccgaacac gtgcgccgca gcctgcgctc
     4561 catcttccgc cataatttca aggctgacct caaggagcac gcctgcaccc agcggccggg
     4621 atacgccatc ggccacgagg ccgggctgct cctctgcacc tggccgcgcg gcggccggcc
     4681 gaccctgccc tttccatatt cggacgaggt gtggacaggc ttcgagtacc aggtggccgg
     4741 ccacctgatc atggaaggaa tggcggccga ggggttgacc ctggtgcggg cggcccggga
     4801 ccgctacgac ggccggaccc gcaacccctg gaacgagtac gagtgcggca actattacgc
     4861 ccgggccatg tccagctacg ccctgctggc ggccctctcc ggcttccact actcggcggt
     4921 gaccggccgg ctgcgcctgg cgccccgcct ggacggcgcc cgcttccgtt gcttcttctc
     4981 gaccgcctcc ggctacggca gcgtcagcct tgacggccgc ggcctgcgca tcgacgtgct
     5041 ggaaggcgag ctccgggtgc ggcgggtcga gctggactgg aaagggaagc gggtttcccg
     5101 ggagtggccg gtccgggcgg cgcccggcca accggcccgc ctgattttct gaaggcttgt
     5161 ttcaaaaaaa ggcccggcgg ccgaatttcg aagccgccgg gctcgtttct tgaggttttc
     5221 ccgcctacga tttcttcttg aacccctcga gccattcccg gatcaggtcc ttcgaaccga
     5281 gcgcgaaggc cagggccgcc ccgaagcaga tgctggccag cacgatgttg aaagccgtcc
     5341 cgatgaactc ggcggcgatg ccgaccacct gcatcgagag cacggccccg aagatcagga
     5401 cggctatctc ggctatcttg gccagggtat ccgccttgtc cactccggag ttggccgccg
     5461 aggttttaac cactccgcgc aggattttgc ccacgaacat cgcgaagacg aagatcacga
     5521 tgcccagcag gagtttcgga atgaaggcca gcagggtgcc gatgatgttg gtcgggatgg
     5581 cgatcccgcc gatggagagg gccaggaaga ccaccaccag cagcagtacc cagtagatca
     5641 ggttggccac catctccgag gccggccgtt tcacgccccc cttctcgagc agggacttga
     5701 agccggactc ttccatgatc cggtcgatct tcatggtccg gaaggcgtga atgagcagca
     5761 gggtgatgat cttggcgatc agccagccga tcaggaagaa gaccagcacc agcacaaagt
     5821 tggagagacc gtaccagaag cgtttcagca tgtccatcgt gggggcgaag atcagttcca
     5881 tgaccgcggc tcctttcggg ttcgggaagc cggccatccg ccggggaggg ccggaaaacc
     5941 tgcttgatct atattactta aagagccgat ttcaagtcaa gttttccgcc gggtttttcg
     6001 ggttgattcg gtttcccggc cgggccgccg gtgggttata atttcaataa tctctttctt
     6061 tcaaaaggag gaagcatgga agaactcaag gtgaagttca ggaaggggcg ctccttcgag
     6121 gcgtccatcc gccagcaccg gctcccggtt gacctgccgc tgggactcaa ggggagcgac
     6181 accggcccca ccccgcccga ttacctggtg ttgagcctgg cctcctgcgt gggcatgtac
     6241 gtgctcttct tctgcgagcg ttccggcctc tccccggagg gactcgaggt ccaggccgag
     6301 tatgccaagg accaggaacg catcaccagc atcgccgttt cggtccgcct gccctcggcc
     6361 gagaccgagg agcggcggcg cgaggcgctg ggctgggccg aaaagtgcct gatccaccgg
     6421 acgctctgcg agcggcccga cctgaagatc agcctgcgct gagccgggtc agggcgaaga
     6481 ccggccgcgg cggggccggg agatgatgat cagcaggaac tccagggcca gccgcggcga
     6541 gacgcggccg cgcaggatgt cctgccgctt ccggagcacc tcgtccagca gggcggccag
     6601 ttcggccgtg tcccgggaac ggtcggccag ttcccgcacc gccgccggcg cgttttccag
     6661 ggcggtgccg gccttcagct tcagggccag gttcagagag ttgaccagct cgatcaggaa
     6721 ggagtccatt ttctccgggt cctccttctt gcccgccagc tcctgggcca gcgcgaatat
     6781 ttcggtcggc cgggaccagc tgagggagag aatccggttc atccggtcgg tctccggcac
     6841 cgccgcctcc gcgtccggaa ggcgcagctg attcaggcgc gagaggacgg tcggcatgaa
     6901 ctggcgctcg gaaggagcca gcagcacgat gaccgcgctt tggggcggct cttccagcac
     6961 cttcagaaag gcgttggccg cctcctcccg gatggcctcc acctccagca ggtagacccg
     7021 gtgttccgcc tggtaggggc tcaggttgag cgattccttg agctggcgga cctgctcgat
     7081 cttgagaaga ccctgttcgc cggccccctc gatgaatttg aagtcggggt gggattcttc
     7141 cttgatgctc cggcattgcg ggcagcggtt gcagggaggg cgtccttcgg tccgcctgga
     7201 gcagaagagg gcctggacga agaaatgggc cagttgaaac cggtcatcgc ggccaccgcc
     7261 gctgatgagc agtggccgct ggtagcggcc ggtgtcaacc gtcttcagga tcagagcttc
     7321 gatttccggg cgagtcattg gcttttttcg gtccggcgtt caatcgctga ccagcaggtt
     7381 cgagagttcg ttgcgcacgc ggcggaagac ctccggccgg ctgccggagg cgtcgatcac
     7441 ccggatccgc gccggttcct gccgggccag cgtcaggaac ccgttccgga cccggcgctg
     7501 gaattcgagc ccggccgcct cgaggcggtc gggttgtccg cagcgggcca gcgccttgtc
     7561 cggatccagg tccagcagga tggtcaggtc cggctgcagt ccgtcgacgg ccagccggca
     7621 ggcgtcggtc agttgcttga gcggcagacc tcgtccgtag ccctggtagg ccagggtgga
     7681 atcggtgaag cggtcgcaga cgaccacccg tcccccggcc cgggcgggaa gaatgactga
     7741 ggtgagcagg ctgcggcggc tggccaggta gaggaaggcc tcggtccagg agtccaggtc
     7801 cggttcgccc gggcgccgtt ccagcaggac ccggcggatc gcctcacccg ccggggtgcc
     7861 gcccggatcc ttggtcagca gcgtattcag gcccaggtcg cgcaggtacc cggccgccag
     7921 tttcagctgg gtgcttttgc cgcagccgtc ggtaccctcg aaagtaatga aaaaaccccg
     7981 tttcatccgc cctccccacc cgcgaccgtt ccgctgccgc cccccggtaa tatgtatatg
     8041 ataccgggcg ccgaacgatt cagccggctt gttgataaac tgtcgagaac cgattgaaaa
     8101 acggttcaag agtcagttat ttcctgaatg aaaagttcct gaaagcccag ttttttcgcc
     8161 agtttaacca gcttcatgta ctcggccggc tccagccggc gggccagttg tggataaccg
     8221 gcggcccggt gggccggaaa gtactgcgac atcaggctga cggtcagttt cgtcgagacc
     8281 gcgcgggcca ggaagcgcag gacgcggtcg ctccgggcca ggttgtcggg caggaccagg
     8341 tggcgcagga taaccccgct taccgccacg ccctcccggt cggtccgcaa agggccggcc
     8401 tggcggaaga tttcccggat ggcccggccg gcccgggtga agtaatcgtc cacgcccgac
     8461 agctcccggc cggcagcgtc atcaccatat tttaaatcgg gcaggtagac ggcgacaacc
     8521 ccctccagcc aggccagcgt ctccaggctc tcgtagccgc tgctgttgta aacgatcggc
     8581 agccggaagc cgcggccgcg ggcctgaacg agcgcttcca ggaactgcgg cacgtaatgg
     8641 gtcggcgtga ccaggttgag gttgtgggcc ccccgttcct ggagttccag catcatggcg
     8701 gccagttcct cgatttcgac ctcgcgtccc tcgcgccgct ggctgatggg gaaattctgg
     8761 cagaagaggc agcttaaggg acagccggag aagaagatgg tgccgctgcc ccggtggccg
     8821 gagatgggcg gttcctcgcc gaagtgaagg ttgtggctgg cgacccggac ccgggcgtcc
     8881 attccgcagc ggccgcgccg gccggcccgc cgatcgatcc ggcactcgcg cgggcagagg
     8941 cggcaggccg agagcgcggc gcgggccgca ttggcccgcc gggataattc ggtccgggtg
     9001 atcggtttga agggcatcgg aatcgggtgg ttcagaaaag gtccaccggg tccaggtcca
     9061 gggtcaggcc gccttcgtaa aggcgcttct ccggcaggga gttcagcagg gccgggatgt
     9121 cgccggggcg gccgcttttg agcagcaggt gccagcggta ccggttccgg agcttcttgt
     9181 ggtagcaggc ggccgggccc agcagctcca cgcccagccg ctcggccgcc ggagtcagca
     9241 gctccagcca ggcgcgcgcc ttgcgctcgg cggtctcctc cttgcggccg ctgaagacca
     9301 ggttggccag ccggccgaag ggcgggtagc ccagctcgcg ccggtactcc agctcctgcc
     9361 ggtagaagcc ttccaggtcg taagcggcca gggcggtcag ggcgtaatgc tccgggttgc
     9421 cggtctggat gagcacctcg gcgttgggca ggctgcggcc ggcccggccg gccagctgga
     9481 agagcagctg gaagtaacgc tcggccgccc ggaaatccgg ccggttgagc aggttgtcgg
     9541 cccccagcac cgcggccagg gtcacgcccg gaaagtggtg ccccttggtg atcatctggg
     9601 tgccgatcag gatctgggcc cggccgctcc ggaacttctc cagttcggcc agtccgccgc
     9661 tccggctcaa agtgtcggag tcgagccgga cgatggccgc gtccggcagc cgccgctgca
     9721 gctcccgttc cagggcctgg gtgccggcgg cgcggcggcc caggttgacg gctccgcagg
     9781 ccgggcaggc cggcggcggt tcccggcggt agttgcagta atggcagtag agttcgccgg
     9841 tggtccggtg gaagaccagg ttgaccgcgc aggtcgggca ggtgaaacgc tggccgcagt
     9901 tggggcagaa gaggtaggtg gtgtagccgc gccggttgac gaagatgatc gactgctcgc
     9961 ctttgagcag ccgttcctcc agcaggctga gcaggcgcgg ggagaccagt cccttttcgc
    10021 ttcggcgccc ccggccgtcg gccgggccgg cccgcaggtc cgagatgtag attttgggca
    10081 gttccagccg gccgatgcgc tcggtgaggg tgaaggaccg gatttcgccg gcgccggccg
    10141 cccgccaggt ttccagggcc ggggtggcgc tcaccagcag gaccagggcg ccggccagct
    10201 ccccccgctt cagggccgcc tcccgggcat ggtagcgggg ctggcgcgtc tccttgtagg
    10261 agcggtcgaa ttcctcgtcc accacgatca gtttcagccg ggcgaagggc gtgaagacgg
    10321 ccgagcgggt gccgatgacc accgaaatcc ggccggcggc cgcgtcccgg aagatccggt
    10381 aatggcggct ccggcttaga cggctgtgca ggacggccgc cttctccccg aagcgttccc
    10441 gggcccagct ttccacctgg ggggccaggg cgatctccgg caccaggatg aggctctgac
    10501 cgcccaaaga cggcgagtcc ttcggaccgc ctaaatacgg cgagtcctgc ggaccgccca
    10561 aagacggcga gtcctgcgaa ccgccggcct cggccagccg gccggccagc tgcaggtaaa
    10621 cctcggtctt gccggtggcc gccggtccct tgagcagggc gacccgcttg ccgtccgcgg
    10681 cggccagctt ctccagctcg gccaggacgg ccgcctgctg ttcggtgggc ggccagtggc
    10741 gcaggccgcc gatgattccc tggctgaact cctcggtctc cgcctcggcc ggggtgcgct
    10801 tgggcggctg gagcgaggcg gccagggcca tgcccagcac ctcgcccagc ggcgcgcagt
    10861 agtaatcggc cagccagccg gccagccgga agatc
//
LOCUS       DAOQYN010000042        10745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_6603_length_10745_cov_4.429654, whole genome shotgun sequence.
ACCESSION   DAOQYN010000042 DAOQYN010000000
VERSION     DAOQYN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10745
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6603_length_10745_cov_4.429654"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   1..2392
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..28
                     /rpt_unit_seq="cccctcgtgcgggggcgtggattgaaac"
     gene            2484..2555
                     /locus_tag="PKN80_02895"
     tRNA            2484..2555
                     /locus_tag="PKN80_02895"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2516..2518,aa:Thr,seq:tgt)
     gene            complement(2833..4443)
                     /locus_tag="PKN80_02900"
     CDS             complement(2833..4443)
                     /locus_tag="PKN80_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNQ34993.1"
                     /translation="MLSRRLALFSTLLTLLILAAVPARAEVRLPSVFGDHMVIQRDLA
                     APIWGWADPGETVTVTIGGKKKSAAAGPNGRWQVKLDPMAAGGPHEITVAGSKNSITI
                     KDVLVGEVWVCSGQSNMGVRVRSSINSDTEIANANYPRMRLFQIKQDFSAEPKTDLPA
                     SWQVCSPETVSGFSGVGYFFGRDLHRELDVPVGLIYGCWSGAWAEAWLSRDGLANPTV
                     KGVIENWWGKISDSYPGQLKKYNEDLAKWEETVKQAREKGETPPRKPAPPFDPANSYL
                     TPGFLYNAMIAPVAGYSIRGTIWYQGEGNIRWGYRYRKIFAALIRDWRRQWGQGDFPF
                     LFVQIAGWRDVPKEPVESSFSELREAQLYTWQTVPNTGMAVTLDIGGGVVHFRNKQEV
                     GRRLALAALANTYGRKNLAYSGPVYRSARREGDKLRLEFDHLGGGLVTSDGGPVQSLA
                     IAGEDKKFVWAESKIEKDTLVVWSPKVTEPVAVRYGWGENPPINLFNKAGLPASPFRT
                     DTWPMNNQREEEAYEKKVAEAAAAPAAK"
     gene            complement(4860..6128)
                     /locus_tag="PKN80_02905"
     CDS             complement(4860..6128)
                     /locus_tag="PKN80_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ34994.1"
                     /translation="MAETRNDEKISSRDREILRRLAEAQAAIADLPVQSENAERWRRV
                     NALQPARPPVLIFEIPWGEYDIPELALQAEHPFCRNLELNFRRLLYQWRNFPGEMVVE
                     NRLYCPLVIRDSGFGLAVKERTIPQGPAAHISSHLYEAQLQDEADLEKIKPPVITLDR
                     ETTELRFQQMRDLFDGILPVEKRGRAGGWFAPWDLLVRWWGVEAALADLVLRPALIHQ
                     AMDRLVNAYLAGLDQLESQNLLALNNANDRVGSGGLGYTDQLPAPGFDPAQVRTIDLW
                     GGATAQIFSEVSPRMHEEFALEHELPWLARFGLNYYGCCEPLHHKIGILKRIPRLRKI
                     SMSPRARLAEGAAAIGGRYVFSLKPNPASLAGDDWNPERVRAELRQALEAARGCAVEI
                     ILKDISTTRGDPRRLADWARVAAEVTAAFA"
     gene            complement(6168..7421)
                     /locus_tag="PKN80_02910"
     CDS             complement(6168..7421)
                     /locus_tag="PKN80_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ34995.1"
                     /translation="MEKREALKRELLTQLYSLRTVDGHSHTYAPDVYYAREINLFNLQ
                     HYFQRDAAGLIGPWEMDAIEDPGECWRRLKTALESAGNTAYWRHQLLVYRKLFDFTDP
                     ELDDANWRRLDTDIRRKTSDPNWYDYVTRSVCNLEIQVRNVGWGEAWDRRYFAGVLRM
                     ENALRLDSAEGRTALEKRLDRPIDSLKAARAGLADLASAARSDGAVGLKLAHAYFRTL
                     DSRPVTETRATRLFEKSLGPGPLDPAEARELQDHFIFYLAELCAELSLVFQIHTGVQT
                     TWGAIPESDPLLLLPLLNRFRAVRFDLFHAGYPYARMLGMLGKHYPNAWLNLAWIYLV
                     SPAASRQVLSEWLDLVPIHRLIGFGSDVHFPELIWGHLEMARDCVAEVLTDKVTQDYW
                     SEGLARDAARKLFRENGLELYALKK"
     gene            complement(7456..8601)
                     /locus_tag="PKN80_02915"
     CDS             complement(7456..8601)
                     /locus_tag="PKN80_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNQ34996.1"
                     /translation="MRSPLILCLLLFCLLVPAELPAQQISSFPVEKTADELFASGSAL
                     AGDGRYAEAIPYLEEAGRRRPNFHQAQYQLGLCYLKTGRYDQARQVLTSVKVLAAEPT
                     LKKAAADLIDSIQAEIENEKRREEEGRRSQEAAEYARAQARRRQAETAAGRQRLQEEA
                     RAEEAAREQLRALETRRPAAAAAPDRKPREIYANAKDAVVYLTVQRSGFPPILGTGFF
                     ISPNLVVTNLHVVYGAEPGRITVLGGNRQFSVRDIYLDSSGIDIAVLTTIENSNYFLP
                     LESAANPEIGEEVYTIGNPIGFPLTFSNGLVSGIRNQEQYIQTTAPVSQGSSGGPLIN
                     QQGRVIGIITGTFQGTQNLNFAVPVKFIPARFRLEAVRAGEQRSSRP"
     gene            complement(9001..9996)
                     /locus_tag="PKN80_02920"
     CDS             complement(9001..9996)
                     /locus_tag="PKN80_02920"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ34997.1"
                     /translation="MIRRLGLTVLALGFFTLIFSSRPAFAADGYLHVTSTPAGATVLV
                     DGKEAGKTPLLTTAAAGKHAVLVTLENYKNATQSVTIVAGQVARLDLTLEKETTPTTN
                     GRRWGDIFRGGKGNLTVTSDWKNTQVYIDGQKHTQNAPFTIKDMESGFHSVILVNGDY
                     AIHDRVLVQNGKSVTVEKSFEELKKSWTTASATASAKEALAAKYAALPARITIQLVKS
                     TSKTSKSDQPFSFQYRKTGTEEWTVKELEWGAKETETFEVEKGTYEFQMIAYHYKDED
                     ILGGILTQRKKVGEGRVTLTKELVRDTLYTFTLTYDGKSSLTNKLEEKAINTKLE"
     gene            complement(10292..>10745)
                     /locus_tag="PKN80_02925"
     CDS             complement(10292..>10745)
                     /locus_tag="PKN80_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNQ34998.1"
                     /translation="LLNEISLGRLEAVETAVLERCRRLAYPLPERELKPLTIEAWRQV
                     SELLAGPAGPIVRPRPDGQSELPFVLNRPDGSIPGRMDRVIITDDTVRIYDYKTGPVR
                     PEKLAEAAAGYRPQLDIYRQAAQSLFPGRRVETHLVFTAAGRIIPVEE"
BASE COUNT         1675 a         3059 c         3907 g         2104 t
ORIGIN      
        1 cccctcgtgc gggggcgtgg attgaaacca ttataccctt ctgatatggc gccctcgctg
       61 tggtcgcccc tcgtgcgggg gcgtggattg aaacataagt gccttcctgt atccagatgc
      121 gattcccggt cgcccctcgt gcgggggcgt ggattgaaac atgtctgcgg cgagaagttt
      181 gcgaccgaca atggtcgccc ctcgtgcggg ggcgtggatt gaaacgcaag acggcgcgtg
      241 agatatttga ccgcctgggt cgcccctcgt gcgggggcgt ggattgaaac gaacgggagc
      301 ggctacacga caccttctct tgtgtcgccc ctcgtgcggg ggcgtggatt gaaaccggga
      361 cgtggatgac tgcttcgtag aggcataacg tcgcccctcg tgcgggggcg tggattgaaa
      421 cagcgtcaaa aaagccaact acgctacctt cccgagtcgc ccctcgtgcg ggggcgtgga
      481 ttgaaacacg acggactttg attttaattc ctacggttcc tgggtcgccc ctcgtgcggg
      541 ggcgtggatt gaaacgccct gtggcaagcg accggccaag aacctctcgt cgcccctcgt
      601 gcgggggcgt ggattgaaac cacctcccga gttcgcgtcc cgctcaaccc cgagtcgccc
      661 ctcgtgcggg ggcgtggatt gaaacaagga taaggatctg accgcaccgc caggatcacg
      721 tcgcccctcg tgcgggggcg tggattgaaa cacgcccgcc ggggccgtgc cgacaaccga
      781 cacaagtcgc ccctcgtgcg ggggcgtgga ttgaaacccg tcaaagtagg ccacgtctcc
      841 agccgcctga gtcgcccctc gtgcgggggc gtggattgaa acatcggaac gggcgtcacc
      901 gtcaatatcg cctacgtcgc ccctcgtgcg ggggcgtgga ttgaaacgcc ggataaggat
      961 ccgtcgaatc gagggggact ggtcgcccct cgtgcggggg cgtggattga aacaaagggc
     1021 gcgagaccgc gatgttccgg ctgaaacgtc gcccctcgtg cgggggcgtg gattgaaaca
     1081 tctctttttc gccatggaag ccgctcacac gcgtcgcccc tcgtgcgggg gcgtggattg
     1141 aaacgtatag caccgagccg cgcctgcgaa ttgcggcgtc gcccctcgtg cgggggcgtg
     1201 gattgaaaca tcgtcccctc gccttttcca gctcgatcct gagtcgcccc tcgtgcgggg
     1261 gcgtggattg aaactttcac caccgcttcg gcccggtcgc agatgtgtcg cccctcgtgc
     1321 gggggcgtgg attgaaactt tcgggcggcc gtgcttgccg gttcgcttct gcgcgtcgcc
     1381 cctcgtgcgg gggcgtggat tgaaacgcgg agatgccgga aaccatccgg gcgaccggcg
     1441 tcgcccctcg tgcgggggcg tggattgaaa ccgtaggatg cgcctcatca caacttgcgc
     1501 aatagtcgcc cctcgtgcgg gggcgtggat tgaaactcgg ccttgatcgc ctcccaccgc
     1561 gttgccagtg tcgcccctcg tgcgggggcg tggattgaaa ctatgtagca ataccagccc
     1621 aggtactggc ggagagtcgc ccctcgtgcg ggggcgtgga ttgaaacttt ccagtgacgt
     1681 ggatctcccg ccgggcaaga tgtcgcccct cgtgcggggg cgtggattga aacaacggat
     1741 actaccggga caacctatgg ggcgtgggtc gcccctcgtg cgggggcgtg gattgaaaca
     1801 gcggatatgg acacgaacaa acgaccgata caagtcgccc ctcgtgcggg ggcgtggatt
     1861 gaaacgcttc ctgccccggt agtcccgccg gcttcggtat gtcgcccctc gtgcgggggc
     1921 gtggattgaa acccgtagta ggtcgggccg gtcgggttgg aggccagagt cgcccctcgt
     1981 gcgggggcgt ggattgaaac attgtcaacc cttccaccgg tcttgtcgcc cccagtcgcc
     2041 cctcgtgcgg gggcgtggat tgaaactagt tttttgctta taagataggg gtgtctctcg
     2101 tcgcccctcg tgcgggggcg tggattgaaa cttcaaggaa ggattgctct cgaagttcct
     2161 tcaaagtcgc ccctcgtgcg ggggcgtgga ttgaaacctc cagctcgcgc ccggtgaatt
     2221 cgccgcggag tcgcccctcg tgcgggggcg tggattgaaa ctgctcggag ttcagtatcg
     2281 ccgtcaatcc ctcgcgtcgc ccctcgtgcg ggggcgtgga ttgaaactta ccgtagtggg
     2341 cactacctac acgcgcgtat gtcgcccctc gtgcggggac gtgaaaggag tgattattgc
     2401 cggacgctgg attgaccggg gggcgcggca ggggctaaaa ttgatggtgc gattgggtat
     2461 gatgatcgtg cgaacgacgg cgcgccacct tagctcagcg gtagagcagg gcacttgtaa
     2521 tgctcaggtc gggggttcaa ttccctcagg tggctctcga ccgggcggct tttgattggg
     2581 ggaaggccgc cgggatattt ttttgatcgc ctgaccgggt gcgcttcggc aatcaggaga
     2641 gtcggatccc gatcagtttg aaggaggcga agtcgcgggg gtacgggccc tgtgcggatt
     2701 gtaagccgcc ccggtagggc ggggcggcgt tgtcggttgg catcagccgg cggcgggtct
     2761 tgatggttca gggacccggc cgcctttttt tatgacggct cgtcgccgcc gggccgccgg
     2821 agacgaacgg gcttacttgg ccgccggggc ggccgccgct tcggcgacct tcttttcgta
     2881 ggcctcttct tcccgctggt tattcatcgg ccaggtgtcg gtgcggaagg gagaggcggg
     2941 caggccggcc ttgttgaaga ggttgatcgg cgggttctcg ccccagccgt agcggacggc
     3001 taccggctcg gtcaccttcg ggctccagac caccagggta tccttctcga tcttcgattc
     3061 ggcccagacg aattttttgt cttcgccggc gatggccagg ctctggaccg ggccgccgtc
     3121 gctggtaacc aggccgccgc cgaggtggtc gaactcgagg cggagcttgt cgccctcgcg
     3181 gcgggcggag cggtaaaccg ggccggagta ggccaggttc ttgcggccgt aggtgttggc
     3241 cagggcggcc agggccagcc ggcggcccac ctcctgcttg ttgcggaagt ggacgacgcc
     3301 gccgccgatg tcaagggtga cggccatgcc ggtgttgggg acggtctgcc aggtgtagag
     3361 ctgggcctcg cgcagttcgg agaaggagct ctcgaccggc tccttgggaa cgtcgcgcca
     3421 gccggcgatc tgaacgaaga ggaaggggaa gtcgccctgc ccccactgcc ggcgccagtc
     3481 gcggatgagg gcggcgaaga tcttccggta gcggtagccc cagcggatgt tgccttcgcc
     3541 ctggtaccag atggttccgc ggatggagta gccggccacg ggggcgatca tggcgttgta
     3601 gaggaagccg ggggtgaggt aactgttggc cgggtcgaag ggcggggccg gcttgcgcgg
     3661 gggcgtttcg cccttttccc tggcctgctt gaccgtctcc tcccacttgg ccaggtcttc
     3721 gttgtacttc ttcaactggc ccgggtagga atcggagatt ttgccccacc agttttcgat
     3781 gacgcccttg acggtcgggt tggccaagcc gtcccggctc agccaggcct cggcccaggc
     3841 gccggaccag cagccgtaga tgaggccgac cggcacgtcg agttcccggt gcaggtcgcg
     3901 gccgaagaag tagccgaccc cggagaagcc ggagacggtt tcggggctgc agacctgcca
     3961 ggaggccggc aggtcggtct tgggttcggc ggagaagtcc tgcttgatct ggaagaggcg
     4021 catccgggga taattggcgt tggcgatctc ggtgtcgctg ttgatggagg agcgtacccg
     4081 gacgcccatg ttggactggc cggagcagac ccagacctcg ccgacaagca cgtccttgat
     4141 ggtgatgctg tttttggatc cggcgacggt gatttcgtgc gggccgccgg cggccatcgg
     4201 gtcgagcttg acctgccagc ggccgttggg gccggcggcg gcgctcttct ttttgcctcc
     4261 gatggtcacg gtgacggttt cgccggggtc ggcccagccc cagatgggcg cggccagatc
     4321 gcgctggatg accatgtggt cgccaaagac cgagggcagg cgcacctcgg cccgggccgg
     4381 gacggccgcg agaatgagca gggtgagcag cgtcgagaat aaggccagtc gtcttgaaag
     4441 catggttgcc tccggtgcga gagtggttcc ggggccggaa acggccaccg ggtttatttc
     4501 acctatttta gcattttcag tagattataa caccggggct gcggggcgaa aggctcggcc
     4561 tctccgtatt ttttagacgg gcgtccaaca agcccgtcat acccgcatgc ttcaggcggg
     4621 tatccaagaa gcctgtcgtg cccgtttgtt gtagacgggc atccaatcct taagaggttt
     4681 tgaacgaaaa acaacggata aagacaacta ctggatcccc ttctactaca taaggggatg
     4741 acccctgaag tagaacgctt cacgtacact tcagggtttt ggattggttt ttcagacgaa
     4801 tcgcgccgaa gcgcgtaata tacgcgcggg cggggatgag gcggaaacag ggagcggcct
     4861 caggcgaagg cggcggtgac ctcggcggcg acccgcgccc agtcggccag gcggcgggga
     4921 tcgccgcggg tggtgctgat gtccttgagg atgatttcga cggcgcagcc gcgggccgcc
     4981 tccaaggcct ggcgcagttc ggcccggacc cgttccgggt tccagtcgtc gccggccagg
     5041 ctggccgggt tgggcttgag ggagaagaca tagcggccgc cgatggcggc ggccccttcg
     5101 gccagccggg cacgcgggct catcgagatc ttgcgcaggc gcgggatgcg cttgaggatg
     5161 ccgatcttgt ggtggagcgg ttcgcagcag ccgtagtagt tgaggccgaa gcgtgccagc
     5221 caggggagtt cgtgttcgag ggcgaactct tcgtgcatcc ggggcgagac ctcggagaag
     5281 atctgggcgg tggcgccgcc ccagaggtct atggtccgga cctgggcagg gtcgaagccg
     5341 ggcgcgggca gctggtcggt gtagccgagg ccgccggagc cgacccggtc gttggcgttg
     5401 ttcagggcca gcaggttctg gctttcgagc tggtcgaggc cggccaggta ggcgtttacc
     5461 agccggtcca tggcctggtg gatcagcgcc gggcgcagga ccaggtcggc cagggcggcc
     5521 tcgacgcccc accaccggac caggaggtcc cagggagcga accagccgcc ggcccggccg
     5581 cgcttctcga ccggcaggat gccgtcgaag aggtcccgca tctgctggaa acggagttcg
     5641 gtggtttcgc ggtcgagggt tatcacgggc ggcttgatct tctcgaggtc ggcctcgtcc
     5701 tggagctggg cttcgtaaag gtgggagctg atgtgggcgg ccggaccctg ggggatggtc
     5761 cgctccttga cggcgaggcc gaagccgctg tcgcggatga ccagcgggca gtagaggcgg
     5821 ttttcgacca ccatttcgcc cgggaagttc cgccactggt agaggaggcg gcggaagttg
     5881 agctcgaggt tccggcagaa ggggtgctcg gcctggaggg ccagttccgg gatatcgtat
     5941 tcgccccagg gaatttcgaa gatgaggacc ggcggccggg ctggctggag ggcgttgacg
     6001 cggcgccagc gttcggcgtt ttccgattgg acgggcaggt cggcgatggc ggcctgcgct
     6061 tcggccagcc ggcgcaggat ttcgcggtcg cggctggaga ttttttcatc gttccgggtt
     6121 tcggccatgg ttatctcctg gcgggcggag cgcccgggcg gcggttctta ttttttcagg
     6181 gcgtagagtt cgaggccgtt ctcccggaag agcttgcggg ccgcgtcgcg agccagcccc
     6241 tcgctccagt agtcctgggt gaccttgtcg gtcaggacct cggccacgca gtcgcgggcc
     6301 atctccaggt ggccccagat cagctcgggg aagtggacgt cggagccgaa gccgatcagg
     6361 cggtggatgg gcaccaggtc gagccattcg gagaggacct ggcggctggc ggccgggctg
     6421 accaggtaga tccaggccag gttgagccag gcgttcgggt agtgcttgcc gagcatgccc
     6481 agcatgcggg cgtatgggta gccggcgtga aagaggtcga agcggacggc gcggaagcgg
     6541 ttgaggagcg ggagcagcag caggggatcc gattccggga tggcgcccca ggtggtctgg
     6601 acgccggtgt ggatctggaa gaccaggctg agttcggcgc agagttcggc caggtagaag
     6661 atgaagtggt cctggagctc cctggcttcg gctgggtcga gcggaccggg gccgagggat
     6721 ttctcgaaga ggcgggtggc ccgcgtttcg gttaccggcc ggctgtccag ggtgcggaag
     6781 taggcgtggg ccagcttgag cccgaccgcg ccgtcggacc gggccgcgct ggccaggtcg
     6841 gccaggccgg cccgggcggc cttgaggctg tcgatcggtc ggtcgaggcg tttttcgagg
     6901 gcggtccgtc cttcggccga gtcgaggcgc agggcgttct ccatgcgcag gacgccggcg
     6961 aagtagcgcc ggtcccacgc ctccccccag ccgacgttgc gcacctggat ttcgaggttg
     7021 cagacgctcc gggtgacgta gtcgtaccaa ttcgggtcgc tggtcttccg gcggatgtcg
     7081 gtatcgaggc gccgccagtt ggcgtcgtcc agttccgggt cggtgaagtc gaagagtttc
     7141 cggtagacca gcagctggtg gcgccagtag gcggtgttgc cggccgattc gagggcggtt
     7201 ttcagccgcc gccagcattc gccggggtcc tcgatggcat ccatctccca ggggccgatg
     7261 aggccggcgg cgtcgcgctg gaagtagtgc tggagattga agaggttgat ttcgcgcgcg
     7321 tagtaaacgt ccggggcgta ggtgtgggag tggccgtcaa cggtccgcag ggagtagagc
     7381 tgggtgagca gttcccgctt gagcgcttct ctcttttcca tcgggttgtc ggatttccgg
     7441 tttgggcggc cgctttcagg gtcgggagga acgctgttcg ccggcccgga cggcctccag
     7501 gcggaaacgg gccgggatga acttgaccgg gacggcgaag ttgaggttct gggtgccctg
     7561 gaaggtgccg gtgatgatgc cgatcacccg cccctgctgg ttgatgagcg gtccgccgct
     7621 gctgccctgg gagacgggcg ccgtggtctg gatatactgc tcctggttgc ggatgccgct
     7681 gaccaggccg ttggaaaagg tgagggggaa gccgatcggg ttgccgatgg tatagacctc
     7741 ctcgccgatt tcggggttgg cggccgattc gaggggcagg aagtagttcg agttttcaat
     7801 ggtggtcagg acggcgatat cgatgccgct ggagtcgagg tagatatcgc gcaccgagaa
     7861 ttgccggttg ccgcccagga cggtgatgcg cccgggctcg gccccgtaca cgacgtgaag
     7921 gttggtgacg accaggttgg ggctgatgaa gaagccggtg cccaggatgg gcgggaagcc
     7981 gctcctctgg acggtgaggt agaccaccgc gtccttggcg ttggcgtaaa tctcccgggg
     8041 cttccggtcc ggcgcggccg ccgcggccgg acgccgggtt tcgagggccc ggagctgctc
     8101 ccgggccgcc tcctcggcgc gcgcctcctc ctggagacgc tgccggccgg cggccgtttc
     8161 ggcctgccgg cgccgggcct gggcccgggc atactcggcg gcctcctggg agcgccggcc
     8221 ctcctcctcg cgccgcttct cgttttcgat ctcggcctgg atggaatcga tcaggtcggc
     8281 ggcggccttc ttcagggtcg gctcggcggc cagcaccttg accgaggtca ggacctggcg
     8341 ggcctggtcg taacggccgg tcttcaggta gcagaggccg agttggtact gggcctggtg
     8401 gaagttgggc cggcggcggc cggcctcctc gaggtagggg atggcctcgg cgtagcggcc
     8461 gtctccggcc agggccgagc cggaggcgaa gagttcgtcg gcggtcttct cgaccgggaa
     8521 tgaagatatt tgctgggccg gcagttcagc cggcacgagc aggcagaaaa ggagcaggca
     8581 gaggatgagc gggcttctca tgggcgttcg gcggccggct ttgaacggga agggccgggt
     8641 gttctcctta ttttattccc ccgggtatat cttatcaccg gcgtccggct tcgtaaagca
     8701 ggcggaaggg aggggagaac atgcttttgt gaatctccgc ctacgacttg cggggacagg
     8761 catgagggga tgacgaacga aagatgagac tttaggcggg atacataaaa gtctgtcata
     8821 cccgtatgcc gtagacgggt atccaatcct taaaaggttt tgaatcaaaa aaattgataa
     8881 agacaactgc tggattcccg ccaactatcc gcgggaatga cgagtagagg gggcgccaaa
     8941 aaaggggcgc ggccgtcaag ggccgcgccc cgggtcaaac cgatcccgcc cgccggcggg
     9001 ttattcgagc ttggtgttga tagccttctc ctcgagcttg ttggtcaggc tggacttgcc
     9061 gtcgtaggtg agggtgaagg tgtagagcgt gtcccggacc agttccttgg tgagggtgac
     9121 ccgcccctcg ccgaccttct tgcgttgggt caggatgccg cccaggatat cctcgtcctt
     9181 gtagtggtag gcgatcatct ggaactcgta ggtgcccttt tcgacctcga aggtctcggt
     9241 ctccttggct ccccactcca gttccttgac ggtccactcc tcggtcccgg tcttgcggta
     9301 ctggaaggag aagggctggt cggacttgct ggtcttgctg gtgctcttga ccagctggat
     9361 ggtgatccgg gccggcaggg cggcgtattt cgcggccagg gcctccttgg cgctcgcggt
     9421 ggcggatgcc gtcgtccagc tcttcttcaa ctcctcgaag gatttttcga cggttaccga
     9481 cttgccgttc tggaccagga cccggtcgtg gatggcgtag tcgccgttga ccaggatgac
     9541 cgagtggaag ccgctctcca tgtccttgat ggtgaagggg gcgttctggg tgtgtttctg
     9601 gccgtcgatg tagacctggg tgttcttcca gtcggaggtg acggtaaggt tgcccttgcc
     9661 gccccggaag atgtcgcccc agcgccggcc gttggtggtg ggcgtcgtct ccttctccag
     9721 ggtcaggtcg aggcgggcca cctggccggc gacgatcgtg acgctctggg tggcgttctt
     9781 gtaattttcg agcgtcacca ggacggcgtg cttgccggcg gccgcggtcg tcagcagggg
     9841 cgtcttgccg gcctccttgc cgtccaccag cacggtggcg ccggccgggg tgctggtgac
     9901 gtgcagatag ccgtcggccg cgaaggcggg gcggctgctg aagatgaggg tgaagaatcc
     9961 cagggccaga accgtcaatc ccaatcgtct gatcatgccg cgcctcccgg tgagcgaaaa
    10021 aagcttttgt ctgcatcagt aattattgac gcggccggag agttttgtca aacggcggcg
    10081 gttttttact tgcggcggaa gggggtgagc agtcgtttgt cgaacttgaa gcggagggtg
    10141 aggtaaggac cctggccccg gtaggcgtcc tgtcagggcc tggttaaggt ggcgacagtt
    10201 ggcgccggcc tgttgttgta agttaaaaag ggggcgacgg attttttgcg gcttgactct
    10261 taaaggattt tccaaagatt atcggaaagg attattcttc gaccggaatg atccggccgg
    10321 cggcggtgaa gacgaggtgg gtttcgacgc gtcggcccgg gaacagggac tgggctgcct
    10381 ggcggtagat atccagctgg ggccggtagc cggcggccgc ctcggccagt ttctccggcc
    10441 ggaccgggcc ggttttgtaa tcgtaaatcc ggactgtgtc atcggtgatg atgacccggt
    10501 ccatccggcc cggtatgctt ccgtccggac ggttgagcac gaagggcagt tcggattggc
    10561 catcgggccg ggggcggacg atcggcccgg ccgggccggc cagcagttcg gagacctggc
    10621 gccaggcctc aatcgtgagg ggttttaact ctcgttctgg cagcgggtag gcgagccgcc
    10681 ggcatcgttc cagcacggcc gtctcgacgg cctccagccg gcccaggctg atttcgttca
    10741 ggagt
//
LOCUS       DAOQYN010000043        10684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_6644_length_10684_cov_4.582181, whole genome shotgun sequence.
ACCESSION   DAOQYN010000043 DAOQYN010000000
VERSION     DAOQYN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10684
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6644_length_10684_cov_4.582181"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="PKN80_02930"
     CDS             complement(<1..1251)
                     /locus_tag="PKN80_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNQ34999.1"
                     /translation="MVANQTAILALDLGTTLVKGGLVSGGRVLARGHFPFPVRYDGLK
                     READPEEFRRGLAGLLAGLGRQARKRRLEVLAVGVASQAQTFTALDRRGRPLIPFIVW
                     NDRRAGAAAELLRARFPDFSAGGHQPADSLSFAAKLLHLRRERPEVFSRARRFLLLNE
                     WSLWQLTGFAFGDSTNFGMGGLYDLRAGGLLEERLGFIGVAPEQLARIRTAGAFGRPL
                     RPDWAGAAGLPPGIPVFSCANDQAAAAAGAGVTESLGLVSLGTALVFYLRRPEPARAL
                     TPAQMTGIFPLGGYFLLSVEPSFGDLLDWGKRTFWPDRGWPEFWRRAPRRPAVRAVFA
                     DNQVGFSGLGLETGRADLLAALVELGRERFRVHLAELFPAGTAGREFLAGGGWAASKP
                     LLDRLAVDTGIRWRPAPAECALLGV"
     gene            1383..2579
                     /locus_tag="PKN80_02935"
     CDS             1383..2579
                     /locus_tag="PKN80_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNQ35000.1"
                     /translation="MKLATLLALAGAGLALAANAAAETDLEILNRGNTRFACELYQKL
                     AEPARDNLFFSPNSLSTALAMAWAGARGETSRAMASTLHFDLPPERLHAAYRELLERL
                     GSEKLNELNAANALWAQRGLGFLPEYLNLARNSYRAGLEELDFRKAPEPARKSINAWV
                     ERQTRDRIKDLIPPGVINSQTRLVLTNAIYFKGTWAEKFLEKNTLEAPFQPEAGPPVK
                     ARLMNRQGEFGYFETPELQGLEIPYAGERLSMVILLPREADGLKNLEKRLNAENLSGW
                     LGQMRNREVKAALPRFRLTGEFALAGVLAGMGLGPAFSDRADFSGITGRPDLCISEVV
                     HKSFVEVNEAGTEAAAATGVVMRVTSIRPPRAVPVFRADHPFIFLIRERATGALLFLG
                     RLANPA"
     gene            2886..5666
                     /locus_tag="PKN80_02940"
     CDS             2886..5666
                     /locus_tag="PKN80_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35001.1"
                     /translation="MKRILGLLLLSFLLTAGPARADDARNLLWDGDFDLGYGNNFWGA
                     VYNNQGPNFRDMWSDGAIRLRRTIATRVYRLEEGDYALCAWVRRDPKRPADQPASLTL
                     TLTNFNYYNNEKRNEYRKVFPVPAGTDWQRFGFPLTISAPVGHYFHVEVAGSADLLVD
                     LVSLTPGAALPETRPRPVADLAAGFYNPEETNTYVDGEERVVELLVSNRGPKKRARVD
                     WALYDFRDDLVRSGRLEEEFPTASVTRRRLKLDDLPYNGYRLACSVEGSPVLGDALLA
                     ILPRIDREAVPRWGADANLEPEHADFTLNLMKRLGMKVINTVSAGGGLSRWSLVQPEE
                     NRFVWRDAEVKKAADQGFELMATLWGFKHFSPWVSKKFVTNGKVSDLEGYTAAFNRYL
                     AEFIRRYGQSFRWLQLVDGLHSVGRPAEFEDFYLKSYATARQTAAEVKAEVLVAYHGA
                     YPAWWLQLFETYDPAKIEWICTDSFDRPFYQAEILNGARARGFKPRYMGPTGVGPRDR
                     LRNTSLLVDRITHRGLPAGYFARQFMLHDWLTRTYGTDDLKYGPDLLFLYYDLRLLGQ
                     CLFMPTAGRTGIEYDNSPTLAMQAMAMFKHQVNGRRPVRPFDQPFSVNGRATADTGLF
                     AYPFTDGRRALIVLARTGNEGLESAWRLSGVNFNGLEARDLFAQPIKPAADGSLTAPE
                     LPVYLEIPHARLEATLKELEKLAGAEIPPAAAPKIEVAPYRLEIGPEGGLRLSKEING
                     QPLLLLEGLAGRPGLPKPVITTDQRRLNAELNLDYGGAGLLVAQLTREGVSFFWRVRN
                     SQVRTIEQALSFRLGPAAGGRPVELAGAGRTLAGRLPADGVAADGLLESTEQAGLAGL
                     TANASRLEIKDLAVFTLPEAEGKNDFLPAAGFQLRRRDGNGLLEADYKVSTYRGGGSR
                     GVTDIQLRIDVK"
     gene            5656..6183
                     /locus_tag="PKN80_02945"
     CDS             5656..6183
                     /locus_tag="PKN80_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35002.1"
                     /translation="MSSKPNRFEPAEMPVYQEVQRFRQKWLWAFLLLLFALFSSQFYR
                     VFAHHRLIPRPLLLPLLANGAIPVLLLLLFWKARLEVRFDRGGLNYRFFPFQLRERSI
                     RWEEVGKAYPRTYRPIMEYGGWGIRTNWNGRNVAYNVSGDRGLQLELKNGRRVLFGTA
                     RPDELAAALNSLGRF"
     gene            complement(6202..>10684)
                     /locus_tag="PKN80_02950"
     CDS             complement(6202..>10684)
                     /locus_tag="PKN80_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNQ35003.1"
                     /translation="KLYAFYPDGSLKWEVVVPQADSYGSGAMLGTPAVGSDGTIYLGD
                     SAGNLRAVNPDDGSVQWLTYFIDRSPMMNQPVVHNNGYLYIGCTNSALYGINLSSPGG
                     GKSTLFPSGATPSTPCIGTDGTLYFGVTWQTTYHTYNLYAVNPSLGVIWTYLVGKEMY
                     SSPALGPDGTVYIAGAGGNLYAVNAENGGYRKGAAWPKFQHDTFNTGNALNSTLIRCE
                     PPSYNFGGVSLGGQARKTFTLTNLDTVNHAIGQLSVLEGVNSETEFVISEDLASGQTL
                     APNESKSFTVTFRPVLVGRRSSSIRIPSGEMNMAALVGGNGGGDGKSALLVRVLDGSN
                     SQFLAGVEVTLEDTGRVTDGNGECIFTALDAGDYGIGLARAGYLPTSRNLTLPASGNL
                     FQEFILYPDNYSIPTVAEVASRYPPDANFMDGVDFNVEFTATIAWSGQTPASVRFIAP
                     NRTQEITASDTGPVAATFNMGRDFGVGGRLGVQAVTGGAGISEIKEADFLVIKKMPLL
                     PLRPVDLGGNFSYSASAGLNWNVFSEGADAGAIPSDIPFFGSQPFIMKLIPTISGGAG
                     SDGSAEIGLEFEGLQQEKKIDSGKFAGFSFSLTPKVDIEGEYVAEASEWDWAGYVGLS
                     GEASFSKSWPFVVMAGPVPIPTYAKVSLSVSADLSLGIVTLSPLELNGRFAIGPYVRG
                     SLGVGADEIFAVEGWVGGGADFGLQFPEEPTLEELGVYVNAGFTVYALLFTWETEALH
                     WDWSLIDGAESVSRPLALARVEPRPLPRDYLAHPDYGSFNGGESGRAAFSVAEGGAEV
                     SYAALQTAAFPYPEADLAAADSQLYAVWLTDDPARASLNRTKLVFSKYDGSAWSAPQA
                     VCDDGTADFHPSLVMFSDGWAAVAWENGDRVLPDTAGFEEVKQNLEICAALYDPAAGA
                     WLPPHRFTTNNYLDRNPKISGFDRNHLLVLWTANPNNHVTGNAANPNSMYASYLSERS
                     GYDTNWAPPQMFWSVGYPVLKYDALYMPRQESGMINGLMASLDMDGDTSTTADREIWR
                     VHHYDNQFGFYWDSPNRRTYDSVPDENPQFYIGPEGLSYYMWLKDNQIVKIRAWYGDL
                     YGAVHTFDEYNSNLTGFRVVKSPGGRVAVGWAEASEYGSDLWTIFYDPATAAWGEPQQ
                     LTHDSAVEKFPVFAFFGDDRLVSLYDRKEMSQLQAARTTPGGRAALAYTPSYGPTGLY
                     ALTYAVRTDIALLPGSLTIDPENPAPGQECLLSVTAVNNGNTAANNVPVAFYQGDPAA
                     GGILIGQVVITGLMRPGDSQTVTLLWTVPAEVTGTVTIYALLDPAAAVAGDDTSNNRL
                     ELARVKPDLRAAWLASRSLTAGLLSLTGRVENPGVTAAGPFQVQIRKDRPDGELLYQQ
                     DFDGLAAKAIGEFNYRWESGGVAADWLYLLVDTGGAVDELNEGNNTYILKLNAYQKGD
                     VNRDWKVNIADAIRAEAMFLGIYAPDPGLADMNGDGLVDLVDVKLLLERCASWLETQ"
BASE COUNT         1733 a         3834 c         3458 g         1659 t
ORIGIN      
        1 cacccccagc aaggcgcact ccgccggcgc tggccgccag cggatcccgg tgtccaccgc
       61 cagccggtcc agcaggggct tcgacgccgc ccagccgccg ccggccagga actcccggcc
      121 cgccgtcccg gccggaaaga gttcggccag gtggactcgg aaccgctccc ggcccagttc
      181 caccagcgcc gccagcaggt cggcccggcc ggtctccagc ccgagcccgg aaaacccgac
      241 ctggttatca gcaaaaaccg cccgcaccgc cgggcggcgc ggcgcccggc gccagaactc
      301 cggccagccc cggtccggcc agaaagtccg cttgccccag tcgagcaggt cgccgaaact
      361 cggctccacg ctgagcagaa aatagccgcc gagcggaaag atcccggtca tctgcgccgg
      421 ggtcagcgcc cgggccggtt ccggccgccg caggtagaag accagggccg tgcccaggct
      481 gaccaggccc agcgattcgg taaccccggc cccggccgcc gccgcggcct ggtcgttggc
      541 gcaggagaag accggtatcc ccggcggcag cccggcggcg ccggcccagt ccggccggag
      601 cggccggccg aaggcgccgg cggtccggat ccgggccagc tgctccgggg ccaccccgat
      661 aaacccgagc cgctcctcca ggaggccgcc ggcccggagg tcgtagaggc cgcccatgcc
      721 gaagttggtg ctgtccccga aggcgaagcc ggtcaactgc cagaggctcc actcgttgag
      781 cagcagaaaa cggcgcgccc ggctgaagac ctccggccgt tcccgcctga ggtggagcag
      841 cttggccgcg aaggaaagag aatcggccgg ctggtggccg ccggcggaaa aatccgggaa
      901 ccgcgcccgg agcagttccg ccgccgcccc ggcccgccgg tcgttccaga cgatgaaggg
      961 aatcaggggc cgcccccggc ggtccagggc ggtgaaggtc tgggcctgcg aggcgacgcc
     1021 gacggccagc acttcgaggc gccgcttccg cgcctggcgg cccagtccgg ccagcaggcc
     1081 ggccaggccg cgccggaact cctccgggtc ggcctcccgc ttcagcccgt cgtagcggac
     1141 cgggaagggg aaatggcccc gcgccagcac ccggccgccg gaaaccaggc cgcccttgac
     1201 cagcgtcgtc cccaggtcca gcgccaggat cgccgtttga ttcgccacca tgctcaggat
     1261 attatattat caatggaccg attcctggca accgggccgg aacttttgcg ggccggcggt
     1321 gtccaattcc ccggacgctc ccgcccggag cgaatcgcga acccttaccc gaggaggccg
     1381 agatgaaact ggcaaccctc ctggccctgg ccggcgccgg cctggccttg gccgcgaacg
     1441 cggccgccga aaccgacctg gaaatcctga accgcggcaa cacccgtttc gcctgcgaac
     1501 tctaccagaa actggccgaa ccggcccggg acaacctctt cttctccccc aacagcttat
     1561 caaccgccct ggccatggcc tgggccggcg cccggggcga gaccagccgg gcgatggcca
     1621 gtaccctcca cttcgacctg ccgccggaac gcctccacgc cgcctaccgg gaactgctgg
     1681 agcggctcgg ctccgagaag ttgaacgaac tgaacgccgc caacgccctc tgggcccagc
     1741 gcggcctggg cttccttccc gagtacctca acctggcccg gaactcctac cgggccggcc
     1801 tggaggaact ggacttccgg aaggcgccgg aaccggcccg caaaagcatc aacgcctggg
     1861 tggaacggca gacccgcgac cgcatcaagg acctgattcc gcccggcgtc atcaacagcc
     1921 agacccggct ggtcctgacc aacgccatct acttcaaggg aacctgggcc gagaagttcc
     1981 tggagaagaa caccctcgaa gcccccttcc agccggaggc cggcccgccg gtcaaggccc
     2041 ggctgatgaa ccgccagggc gaattcggct acttcgaaac gccggaactc cagggcctgg
     2101 agatccctta cgccggggag cgcctctcga tggtcatcct gctgccgcgg gaggccgacg
     2161 ggcttaaaaa ccttgaaaag cgtcttaacg ccgaaaatct ctccggctgg ctcggccaga
     2221 tgcggaaccg cgaggtcaag gccgcgctgc cccgcttccg gctgaccggc gagttcgccc
     2281 tcgccggcgt gctggccggg atgggcctgg ggccggcctt ctccgaccgg gccgacttct
     2341 ccggcatcac cggccgtccc gacctctgca tctccgaggt ggttcacaag tccttcgtcg
     2401 aggtcaacga ggcgggcacc gaggcggccg cggccaccgg cgtggtgatg cgggtcacct
     2461 ccatcaggcc gccccgggcc gtgcccgtct tccgggccga ccaccccttc atcttcctca
     2521 tccgggagcg cgccaccggc gccctcctct tcctgggccg cctcgccaac cccgcctgat
     2581 accgattcgc cttcgggccg aatagctttg ttgccggcgt cggcggcggc gtcagcgtac
     2641 cttcagtacg cttcctggcc ttctcctggg cgccgcgctc tcggcccgca ttcaaaccgg
     2701 tattatcctg taatcaagac ttactgatcc cgggccgccc ccgcgcggcc gcgggttgtc
     2761 cggactgaag tggtgttgta taatttcccc ggttccggac caacccgggc catcggtcaa
     2821 ccatccggct tccaggcgtc cggaaacttt ccggccgccg gccgccgctt ccgacggggc
     2881 gtttcatgaa aagaatcctc ggcctccttc tcctctcctt tctcctgacc gccggcccgg
     2941 cccgggccga tgacgcgcgc aacctgctct gggacggcga cttcgacctc ggctacggca
     3001 acaacttctg gggcgccgtc tacaacaacc aggggccgaa cttccgcgac atgtggtccg
     3061 acggggccat ccgcctccgc cgcaccatcg ccacccgggt ttaccggctc gaggaaggcg
     3121 actacgccct ctgcgcctgg gtccgccgcg acccgaagcg tccggccgac cagccggcca
     3181 gcctcaccct caccctgacc aacttcaatt actacaacaa cgagaagcgc aacgagtacc
     3241 gcaaggtttt cccggtcccg gccgggaccg actggcagcg gttcggtttc ccgctgacca
     3301 tctcggcgcc ggtcggtcat tacttccacg tcgaggtggc cggcagcgcc gacctcctgg
     3361 tcgacctggt cagtttgacc ccgggcgccg ccctgcccga aacccggccc cggcccgtcg
     3421 ccgacctcgc cgccggcttc tacaacccgg aggaaaccaa tacctacgtg gacggcgagg
     3481 agcgggtggt ggaactgctg gtcagcaacc gcggccccaa aaaacgggcc cgggtcgact
     3541 gggcccttta tgatttccgg gacgacctgg tgcgctccgg ccgcctcgag gaggagttcc
     3601 cgaccgcctc ggtcacccgg cgccgcctga aactggacga cctgccctac aacggctacc
     3661 ggctggcctg ctcggtcgag ggcagcccgg tcctgggcga cgccctgctg gccatcctgc
     3721 ccaggatcga ccgggaagcg gttccccgct ggggggccga cgccaacctg gagccggagc
     3781 atgccgactt caccctgaac ctgatgaagc ggctgggcat gaaggtcatc aacaccgtca
     3841 gcgccggcgg cggcctcagc cgctggtccc tggtccagcc cgaggaaaac cgcttcgtct
     3901 ggcgcgacgc cgaggtgaag aaggcggccg accagggctt cgagctgatg gccaccctct
     3961 ggggcttcaa gcatttctcg ccctgggtct cgaagaagtt cgtgaccaac ggcaaggtga
     4021 gcgacctcga gggatacacc gcggccttca accgctacct ggccgaattc atcaggcgtt
     4081 acggccagag tttccgctgg ctccagctgg tggacggcct ccactcggtc ggccgcccgg
     4141 ccgagttcga ggacttctac ctaaaatcat acgccaccgc caggcagacc gccgccgagg
     4201 tcaaggccga ggtcctggtg gcttaccacg gtgcctaccc ggcctggtgg ctgcaactct
     4261 tcgagaccta cgacccggcc aaaatcgaat ggatctgcac cgactccttc gaccggccct
     4321 tctaccaggc cgagatcctc aacggcgccc gggcccgggg cttcaagcca cgctacatgg
     4381 gtccgaccgg cgtcggcccg cgcgaccggc tgcgcaacac ctcgctgctg gtcgaccgga
     4441 tcacccaccg gggcctgccg gccggctact tcgcccgcca gttcatgctc cacgactggc
     4501 tgacccgcac ctacgggacc gacgacctga agtacgggcc cgacctgctc ttcctctact
     4561 acgacctgcg cctgctcggc cagtgcctct tcatgcccac cgccggccgg accgggatcg
     4621 agtacgacaa ctcgcccacc ctggccatgc aggcgatggc catgttcaaa caccaggtca
     4681 acggccgccg gccggtgcgc cccttcgacc agcccttctc cgtcaacggc cgggccaccg
     4741 ccgacaccgg cctcttcgcc taccccttca ccgacggccg ccgggccctg atcgtgctgg
     4801 cccggaccgg caacgaggga ctcgaatccg cctggcgcct ctccggggtg aacttcaacg
     4861 gcctcgaagc gcgcgacctc ttcgcccaac ccatcaagcc ggcggccgac ggcagcctga
     4921 ccgccccgga actgccggtc tacctggaga tcccccacgc ccggctggag gcgaccctca
     4981 aggaacttga aaaactggcc ggcgccgaga tcccgccggc ggccgccccg aaaatcgaag
     5041 tggcccctta ccggctggag atcggcccgg agggcggcct gcgcctcagc aaggaaatca
     5101 acggccagcc gctcctgctc ctggagggcc tggccggccg gcccggcctg ccgaagccgg
     5161 tcatcacgac cgaccagcgc cgcctcaatg ccgagctcaa cctcgattac gggggggccg
     5221 gcctgctggt ggcgcagctt acccgggagg gcgtctcctt cttctggcgg gtccgcaatt
     5281 cgcaggtcag gaccatcgaa caggccctct ctttccgcct cggcccggcc gccggcggcc
     5341 ggccggtcga actggccggg gccgggcgta ccctggccgg ccgcctgccg gccgacggcg
     5401 tcgcggccga cggattgctg gaatcaaccg aacaggccgg gctggccgga ctcaccgcca
     5461 acgccagccg cctggagata aaagacctgg ccgtttttac cctgccggag gccgagggca
     5521 agaatgattt tctgccggcg gccggcttcc agttgcggcg gcgcgacgga aacggcctgc
     5581 tggaggccga ctacaaggtc agcacctacc gcggcggcgg cagccgcggg gtgaccgata
     5641 tccagctgcg catcgatgtc aagtaaaccg aaccggttcg agccggctga aatgccggtc
     5701 taccaggaag tccagcgctt ccggcagaag tggctctggg ccttcctgct gctcctcttc
     5761 gccctcttct cgagccagtt ctaccgggtc ttcgcccacc accggctgat accgcggccg
     5821 ctcctgctcc cgctcctggc caacggcgcc atcccggtcc tgctgctcct gctcttctgg
     5881 aaggcccggc tggaagtgcg cttcgaccgc ggcgggttga actaccgttt cttccccttt
     5941 caactcaggg agagaagcat aaggtgggag gaagtcggga aggcctaccc gcggacctac
     6001 cggccgatca tggagtacgg cggctggggc atccggacca actggaacgg ccgcaacgtc
     6061 gcctacaacg tctccggcga ccggggcctc cagctggaac tgaagaacgg ccgccgggtc
     6121 ctcttcggaa cggcccggcc ggacgaactg gccgccgccc tcaactcgct cggccgcttc
     6181 tgaagcgggg cggccgcccg cctactgcgt ctccagccag ctggcacagc gctccagcag
     6241 cagcttcacg tcaaccaggt caaccagccc gtcgccgttc atgtcggcca ggcccgggtc
     6301 gggcgcgtag atacccagga acatcgcctc ggcccggatc gcgtcggcga tgttgacctt
     6361 ccagtcccgg ttgacgtcgc ccttctggta ggcattcagc ttcaggatat aggtgttgtt
     6421 cccttcgttc aactcgtcca ccgcgccacc ggtatccacc agcaggtaga gccagtcggc
     6481 cgcgacgccg ccgctctccc accggtaatt gaactcgccg atggccttgg ccgccaggcc
     6541 gtcgaagtcc tgctggtaaa gcagttcgcc gtccggccgg tccttccgga tctggacctg
     6601 gaaggggccg gcggccgtca ccccggggtt ctccacccgg ccggtcagcg aaagcaggcc
     6661 ggccgtcagg ctccgggagg ccagccaggc cgcccgcagg tccggcttca cccgggccag
     6721 ctccagccgg ttgttggagg tatcgtcgcc ggccaccgcc gcggccggat ccagcagcgc
     6781 gtagatggtc accgtcccgg tcacctcggc cgggaccgtc cagagcaggg tgacggtctg
     6841 ggagtcgccg gggcgcatca ggccggtaat caccacctgt ccgatcagga tgccgccggc
     6901 ggccgggtcg ccctggtaga aggctaccgg gacgttgttg gcggccgtgt tgccgttgtt
     6961 gaccgccgtc accgacagca ggcactcctg ccccggcgcc gggttctccg gatcgatcgt
     7021 cagcgaaccc ggcagcaggg cgatatcggt ccggaccgcg taggtcagcg catagaggcc
     7081 ggtcggcccg taactgggcg tgtaggccag cgccgcccgg ccgccggggg tggtgcgcgc
     7141 cgcctggagc tggctcatct ccttccggtc gtagaggctc accagccggt cgtccccgaa
     7201 gaaggcgaag accgggaact tctcgaccgc gctgtcgtgg gtcagctgct gcggctcacc
     7261 ccaggcggcc gtggccgggt cgtagaagat cgtccagagg tcgctcccgt actccgaggc
     7321 ctcggcccag cccaccgcca cccggccgcc cggacttttg accacccgga atccggtcag
     7381 gttgctgttg tactcgtcaa aggtatgaac ggcgccatac aggtcaccat accaggcccg
     7441 aatcttgact atctggttat ccttcagcca catgtagtaa ctcaaaccct ccggcccgat
     7501 gtagaactgc gggttctcgt ccgggactga atcgtaggtt ctccggttcg ggctgtccca
     7561 gtagaaacca aactggttat cgtaatggtg gacccgccag atctcccggt cggcggtggt
     7621 cgaggtatcc ccgtccatgt ccaggctggc catgaggccg ttgatcatgc cgctctcctg
     7681 cctgggcatg taaagggcgt cgtacttcag caccgggtag cccacgctcc agaacatctg
     7741 gggcggcgcc cagttggtgt cgtatccgct ccgttcggag aggtaggagg cgtacatgct
     7801 gttcgggttg gcggcgttgc cggtgacgtg gttgttcggg ttggccgtcc agagaaccag
     7861 caggtggttc cggtcgaatc ccgagatctt ggggttgcgg tccaggtaat tgttggtcgt
     7921 gaagcggtgg ggcggcagcc aggcgccggc cgccgggtcg taaagggcgg cgcagatctc
     7981 caggttctgc ttgacctctt cgaagccggc cgtgtccggc aggacccggt cgccgttctc
     8041 ccaggcgacc gccgcccagc cgtcgctgaa catcaccagg ctcgggtgga agtcggcggt
     8101 cccgtcgtcg cagaccgcct ggggggcgct ccaggcgctc ccgtcgtact tcgagaagac
     8161 cagcttggtc cggttcagcg aggcgcgggc cgggtcgtcg gtcagccaga ccgcgtagag
     8221 ctgggaatcg gccgcggcca ggtccgcctc cgggtagggg aaggcggccg tctgcagcgc
     8281 ggcgtaactc acctcggcgc cgccctcggc caccgagaag gcggcgcggc cggactcccc
     8341 gccgttaaag ctcccgtaat cgggatgggc gaggtaatcg cgggggagcg gccgcggctc
     8401 gacccgggcc agcgccagcg gccggctgac cgactcggcg ccgtcgatca gcgaccagtc
     8461 ccagtggagc gcctcggtct cccaggtgaa gagcagggcg taaacggtga atccggcatt
     8521 cacgtaaacg cccaactcct ccagggtcgg ctcctccggg aactggaggc cgaagtcggc
     8581 cccgccgccc acccagccct cgaccgcgaa gatctcgtcg gcgcccacgc ccagggaacc
     8641 gcggacatag ggaccgatcg cgaagcggcc gttaagttcc agcggcgaaa gggtgacgat
     8701 gcccaggctc aggtcggccg agaccgagag gctgaccttg gcgtaggtcg ggatgggcac
     8761 cggcccggcc atgaccacga agggccagga cttgctgaag gaggcctcgc cgctcaggcc
     8821 gacgtaaccg gcccagtccc actcgctcgc ctcggccacg tactcgccct cgatgtccac
     8881 cttgggcgtc aggctgaagg agaagccggc gaacttcccg gagtcgatct tcttctcctg
     8941 ctggagcccc tcgaattcca ggccgatctc ggcgctgccg tcgctgccgg cgccgcccga
     9001 gatggtcggg atcagcttca tgatgaaggg ctggctcccg aagaagggga tgtcggaagg
     9061 gatggccccg gcgtcggccc cttcggagaa gacgttccag ttcaggccgg ccgaggccga
     9121 gtagctgaag ttgccgccca ggtcaaccgg ccgcaggggc agcagcggca tcttcttgat
     9181 gaccaggaaa tcggcctcct tgatctccga tatcccagcg ccgccggtca ccgcctggac
     9241 cccgaggcgg ccgcccaccc cgaaatcgcg ccccatgttg aaggtcgccg ccaccggccc
     9301 ggtatcgctg gcggtgatct cctgggtccg gttcggggcg atgaagcgca cgctggcggg
     9361 cgtctggccg gaccaggcga tggtggccgt gaactcgacg ttgaagtcca ccccgtccat
     9421 gaagttggcg tccggcgggt agcgggaggc cacctcggcc acggtcggga tgctgtaatt
     9481 gtccgggtag agaatgaact cctggaagag gttgccgctg gccgggaggg taaggttccg
     9541 ggaggtcggc aggtagcccg cccgggccag gccgatcccg tagtcgccgg catccagggc
     9601 ggtaaagata cactcgccgt tgccgtcggt cacccggccc gtgtcttcca gcgtcacctc
     9661 gacgccggcc aggaactgag agttggagcc gtccagaacc cggaccagca gggccgattt
     9721 gccgtcgccg ccgccgttgc cgcccaccag cgccgccatg ttcatctcgc ccgagggtat
     9781 ccggatcgaa gaactccggc ggccgaccag aaccggccgg aaggtgaccg tgaaggactt
     9841 cgactcgttc ggggccagcg tctgtcccga ggccaggtcc tcggagatga cgaactccgt
     9901 ctccgagttg accccctcca gcaccgacag ctggccgatg gcgtggttga cggtgtccag
     9961 gttggtcagc gtgaaggtct tacgggcctg tccgcccagg ctgacgccgc cgaagttgta
    10021 actcggcggt tcgcagcgga tcagggtgct gttgagggcg ttgccggtgt tgaaggtgtc
    10081 gtgctggaac ttcggccagg ccgctccctt ccggtaaccg ccattctcgg cgttgaccgc
    10141 gtagaggttg ccgccggcgc cggcgatata gaccgttccg tccggaccca gggccggcga
    10201 cgagtacatc tccttgccga ccaggtaggt ccatatcacg cccagcgacg gattgaccgc
    10261 ataaaggtta taggtgtggt aggtggtctg ccaggtgact ccgaagtaga gggtgccgtc
    10321 ggtcccgatg cagggcgtgc tgggcgtcgc tcccgacggg aagagggtcg acttgccccc
    10381 gccgggactg gaaagattga tcccgtagag agcactgttg gtgcagccga tgtaaagata
    10441 gccgttgttg tgaacgaccg gctggttcat catcggcgaa cggtcgatga agtaggtgag
    10501 ccactggacc gaaccatcgt ccgggttgac cgcccgcagg ttgccggcgg agtcgcccag
    10561 gtagatggtc ccgtcgcttc cgaccgccgg cgtcccgagc atcgccccgc tgccgtaaga
    10621 atccgcctgc ggaacgacca cctcccactt caagctgccg tccgggtaga aggcgtacag
    10681 tttc
//
LOCUS       DAOQYN010000044        10194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_7039_length_10194_cov_4.342835, whole genome shotgun sequence.
ACCESSION   DAOQYN010000044 DAOQYN010000000
VERSION     DAOQYN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7039_length_10194_cov_4.342835"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKN80_02955"
     CDS             complement(<1..369)
                     /locus_tag="PKN80_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNQ35004.1"
                     /translation="MREGRRVVITGLGLVTPLGTGVEKTWSGLLAGRSGIGPITQFDV
                     SAFPTRISGTVPDFVAPEFLDRKEIRRLDRFVQYALIAAAEAMNDAGLSREKIDPART
                     GVIVGSGIGGLRVMEQEHRNL"
     gene            complement(390..632)
                     /locus_tag="PKN80_02960"
     CDS             complement(390..632)
                     /locus_tag="PKN80_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNQ35005.1"
                     /translation="MDRSEIEKVVKEVVVEQLAVDANEVNINASFVDDLGADSLDTVE
                     LVMAFEEKFGLEIPDEEAEKIKTVGDAVNYISSKKA"
     gene            complement(704..1438)
                     /gene="fabG"
                     /locus_tag="PKN80_02965"
     CDS             complement(704..1438)
                     /gene="fabG"
                     /locus_tag="PKN80_02965"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014095486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNQ35006.1"
                     /translation="MLLEKKTALITGAARGIGAAIARLFAAEGADLVLWDLAPLDEIS
                     LELKQTGRHIWTDKVDISDLGAVEEAVKRLDPEPGRLDVLVNNAGVTRDNLLFRMKAE
                     EWDLVLKINLYGAFNCTKVLGRLMARKGGGSIINLASVIGLRGNPGQANYGASKAGLI
                     GFTKSSARELARFGIRVNALAPGYIQTPMTDRLTEEQREAIRKLIPMEVFGKPEDVAE
                     VALFFASRLSGYVTGEVLRIDGGFAM"
     gene            complement(1452..1715)
                     /locus_tag="PKN80_02970"
     CDS             complement(1452..1715)
                     /locus_tag="PKN80_02970"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HNQ35007.1"
                     /translation="MKHGLGFFLLLLLISALIGSAVGEIAGFLLGPESPAYQFFTSAA
                     TPALNPSELDLILVKLTFGAALHLNLTALLGMIIATIAYLRLP"
     gene            complement(1726..2793)
                     /gene="trpD"
                     /locus_tag="PKN80_02975"
     CDS             complement(1726..2793)
                     /gene="trpD"
                     /locus_tag="PKN80_02975"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNQ35008.1"
                     /translation="MKDLIARLVRKEDLSQEEAARGMTLIMEGRATEAQIAAFLMGLR
                     LKGETSDEITGCARVMRQKALRIDIDREVIDLDRDDINVDRETIIDTCGTGGDGTNTF
                     NVSTATAFVVAGAGCLVAKHGNRSVSSSCGSADVVRSLGINLDLEPAEVKQCVEKVGI
                     GFFYAPLYHSAMKFALPVRVQLGIRTIFNLLGPLSNPAGANVQVMGVYSRELTTLMAE
                     VLRNLGCRSALVVYGEGSFDEISIVGRTFIAHLKEGAVSNYQVSPEDFGFAPAGLETI
                     RGGSAEVNARIILEVLGGTPGPRRNMVLMNAAAVFLAAGRVADWKAGIALAGHTIDSG
                     QALGRLEALIEFTNRFAVKEK"
     gene            complement(2819..3382)
                     /locus_tag="PKN80_02980"
     CDS             complement(2819..3382)
                     /locus_tag="PKN80_02980"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNQ35009.1"
                     /translation="MTLLIDNYDSFTYNIFQALSELGARVKVVRNDRIESAAISRLRP
                     DRIIISPGPGRPEDAGISVEVIRRFAGKLPILGVCLGHQAIGYAFGGRIIRAPTLMHG
                     KVSMIRHRGQGVFLGLPDPFEATRYHSLVVDRKSLPASLQVTAWTDSGLIMGLQHENF
                     QVFGVQFHPESILTGEGSKLLANFLRV"
     gene            complement(3433..4920)
                     /gene="trpE"
                     /locus_tag="PKN80_02985"
     CDS             complement(3433..4920)
                     /gene="trpE"
                     /locus_tag="PKN80_02985"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNQ35010.1"
                     /translation="MEITPSFDEFRRLARDYNLVPLSVEILADLETPVSAYWKVVGAR
                     PGFLLESVEGGEKQGRYSFLGTDASLIISAVGDRVSLDRRGRVETRSVEPLLFLRDLM
                     SGFRVAPDPGLPPFSGGLVGYCSYDLVRRFERIPDRNPDDLGMPDFQFFLADTLLAFD
                     HFRHRLRVITHAEIDSDVENAYRKAVLRLESIAGRLRKTGTPYPGGPASASDRFRMKP
                     DWDRKDFEAAVRRAKSYIRRGDAIQVVLSQRLAIDFPGSGANQFNLYRAIRSINPSPY
                     LFHLNFGPFSISGSSPEVMVRLEADRATLRPIAGTRPRGADEVSDLALERELRRSRKE
                     QAEHIMLVDLGRNDLGRVCRPGSVKVTELMSVERYSHVMHLVSNITGRLVPGRDALDL
                     FQACFPAGTVSGAPKVRAMEIIDELEQKRRGPYAGAVGYLDFAGNLDTCITIRTVLVK
                     GGRAYLQVGAGIVADSDPGREYEETMNKGRALLESLRLVLTGQLC"
     gene            complement(4935..6509)
                     /gene="nadB"
                     /locus_tag="PKN80_02990"
     CDS             complement(4935..6509)
                     /gene="nadB"
                     /locus_tag="PKN80_02990"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNQ35011.1"
                     /translation="MIPAGLLDFDTLSLPEEETGVLVIGGGVAGLSAALSAAGRRGVT
                     LAMKSGLLDSASETAQGGVAAVLSSSDSFENHLKDTIAAGAGLVSEAAARLLVEEGPE
                     CIAELLEFGASFNRKGRELAFAREGGHSAARIIYAAGDRSGAELVRTLSSRVLQTREI
                     EIRERYFLIDLLASGHNVVGGLFYEREKRALLVIRAASTILATGGVGQLYQETTNPSI
                     STGDGMAAAYRAGAVLTDLEFIQFHPTVLYIAGAPRFLISEAVRGEGAYLINDRQERF
                     MPHYHPAADLAPRDVVTRAILDQMRRRGIGNVYLDCRPVASIFRKRFPTIYNIVCRLL
                     LEKKKEEIPVRPAVHYHMGGVKSDLEARTTLNNLFVCGETAATGVHGANRLASISLLE
                     CLVFGRRAGRFAAENGERSRVRVRYSRRWNDFQVDFNDLRRSLNTLMWRQVGLERNGF
                     DLELALEKINNWQSYALTSRVADPSGWEIQNMLWLAGLITRGALERTESRGAHFRTDF
                     PERDDARWLRHLERRI"
     gene            complement(6511..7059)
                     /locus_tag="PKN80_02995"
     CDS             complement(6511..7059)
                     /locus_tag="PKN80_02995"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ35012.1"
                     /translation="MRINGKKGFTLIELLVVIAIIAILAGMILPALSRAREQARRTTC
                     INNLKQLGMSLFMYARENDERMPSAGAWWDEISGYLAEGSYLCPSAVGSDRDNNVNYG
                     YNANAAGYSVADVAESSSFAIFFDQTGSTFSGTPTDSGFFYDICTDRHDGGANFFFAD
                     GHVAWVKDPQNASASVLKFTRN"
     gene            complement(7149..7697)
                     /locus_tag="PKN80_03000"
     CDS             complement(7149..7697)
                     /locus_tag="PKN80_03000"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNQ35013.1"
                     /translation="MGFRSISRGGVGWRLYGRLDSTQDEAWRWLETDPPPSGSLVLAF
                     RQTAGRGRLGRDWQSPPGGLWFSILLYPVPSVQAGGLLLAAAYQLADSVFRLTGRRPA
                     VKLPNDLYLESRKLAGLLLERRGDRVVLGIGLNVNCSIQSRAGNFKAISLRETVGVRC
                     SRQRILDDFLAGFQRRLPGRFF"
     gene            complement(7738..9681)
                     /locus_tag="PKN80_03005"
     CDS             complement(7738..9681)
                     /locus_tag="PKN80_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35014.1"
                     /translation="MENQNQLTPERFENGFNAKAPIAALFVGFIMMPAAIYMGLIAGT
                     SLGPAAQWVTLILFIEIAKRSFITLKRQEIYIILLATAALAGGGAFGPVMYNQYFIQS
                     PYAKAFGLTDLIPRFAVPPAGSPALLQRTFFHSDWVKPIAILVTTSTLHLLNSLTLGY
                     IFFRVTSDFERLEFPMAPVQTQGVLALADLAAKKDTQRWRIFTIGAMLGVIYGAFYVF
                     IPTITGLTMSKPFMLIPIPFVDFTSQLGSRFPAAIFGMVTDLGSFLIGFVLPFWVVVG
                     HFIGSVGAKTIANPLIYKHTDFLKTWQPGMGAIPASVSTTIDFWVSVTIGTGLAVFVI
                     SIISIFIRLRSARGKGHLEKTAAPAVVADRGDYPIWIAIGLWFFATLAYVLLVRYLVP
                     DFPIWIPVSFGFLLTPALTYISARMYGITGVSDQTSFPYLIEGTYILSGYRGAAIWYA
                     PLPYFNHGGIAQGFKQMEILKIRFTSWYKTAAMTLGIMLFCSFTFWSIIWRMAPIPST
                     IYPYVQRMWPQQAMMQALWISSTVESSVGRSFLLGGLKKAVIGWTFLTSLGLAGIFSL
                     VKWPMALFYGILGGIPMLPHNAVPMFLGGMIGRYFFARRIGPGWRNMTPLLTAGYGCG
                     IGLAGAASVAIALIAKTVFQLVF"
     gene            complement(9688..>10194)
                     /locus_tag="PKN80_03010"
     CDS             complement(9688..>10194)
                     /locus_tag="PKN80_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35015.1"
                     /translation="ARKASQLSVPDVTRRWVLPRPTGDTWLFEFPFTVGQTEVLGLMT
                     FLRDYFASYSIEATGSFYTSDARFNAEKTERGSAYITGCNVSLAPFDLGVSQTVQLRA
                     YPMSEFVFYGIQVEIRRENGERGDWQRLNRRFLDSIRKQFLVWRTLPPEVKDEYRQQG
                     EKVIANGK"
BASE COUNT         2153 a         3141 c         3115 g         1785 t
ORIGIN      
        1 caggttgcgg tgctcctgtt ccataacccg cagcccgccg atgccggacc cgacgatcac
       61 gccggtccgg gccgggtcaa tcttctcccg gctgaggccg gcgtcgttca tcgcctcggc
      121 cgcggcgatc agggcatact ggacaaaacg gtccaggcgc cggatctcct tgcgatccag
      181 gaactccggc gccacgaaat caggcaccgt cccggaaatc ctggtgggaa aggcactgac
      241 gtcgaactgg gtgatggggc cgataccgct gcgcccggcc agcagaccgg accaggtctt
      301 ttcaaccccg gttcccagcg gggtcaccaa ccccaggccg gtaataacga ctcgtcttcc
      361 ttccctcatg tcggattgaa accgggtggt caggctttct ttgaggatat gtaattgacg
      421 gcatcgccga cggtcttgat cttctcggcc tcctcatcgg gtatctccag cccgaatttc
      481 tcctcgaagg ccatgaccag ctcgaccgta tccagcgaat cggcgccgag atcgtccaca
      541 aacgaggcgt tgatgttcac ctcgttggcg tcgacggcca gctgttcaac caccacttcc
      601 ttgaccacct tttcgatttc gcttctgtcc atactctcct ccaccttggg tgacccggta
      661 aatacttccg gctaaaaaaa caaaaactaa atggttaatt atattacatc gcgaaaccgc
      721 cgtcaatccg gagcacctcg ccggtcacgt atccggagag acgcgaagcg aagaagagcg
      781 ccacctcggc cacgtcttcc ggttttccga aaacctccat gggaatcagc ttccggatcg
      841 cctcccgctg ctcctcggtc aaccggtcgg tcatcggggt ctgtatgtaa ccgggcgcca
      901 gcgcattaac ccggattcca aagcgggcca gttcacgcgc gctgctcttg gtaaatccga
      961 tcagcccggc cttggaggcg ccgtaattgg cctgaccggg attgccgcgc agaccgatca
     1021 ccgaggcaag attgatgatg ctcccgccgc ccttgcgggc catcagccgg cccagcacct
     1081 tggtgcaatt gaaggccccg taaaggttga tcttcaaaac caggtcccat tcctcggcct
     1141 tcatgcggaa caaaaggtta tcccgggtca ccccggcatt gttgaccagg acatccaggc
     1201 ggcccggttc cgggtccagg cgcttgaccg cctcctccac ggccccaaga tcggagatgt
     1261 caaccttatc cgtccagatg tggcggccgg tctgcttgag ttcaaggctt atttcgtcaa
     1321 ggggcgccag gtcccagagc accagatcgg ccccctcagc ggcaaaaagg cgggcgatgg
     1381 ccgcgccgat tccgcgggcg gccccggtta tgagggcggt cttcttctcc agcagcattg
     1441 aatctccttt cctaaggcag gcgcaggtag gctatggtgg cgatgatcat gccgagcagg
     1501 gcggtcaggt tcaggtgcag cgccgctccg aaggtcagct tgaccagaat caggtccagt
     1561 tcggacgggt tgagggccgg agtcgcggcc gaggtgaaaa actggtaagc cggggattcc
     1621 ggccccagca ggaaaccggc gatttctccg acggcgctgc cgataagcgc tgagatcagc
     1681 agcaacagga gaaaaaaacc aagcccgtgc ttcatgaaac tccccctact tttctttgac
     1741 agcaaaacgg ttggtgaact cgatcaaggc ctccagcctt cccagcgcct gtccggaatc
     1801 gatcgtatga ccggcgagag cgatgcccgc cttccagtcc gccacccgac cggccgccag
     1861 gaataccgcg gcggcattca taagcaccat gttccggcgg ggccccggcg tcccacccag
     1921 cacttccagg ataatccggg cattaacttc ggccgaaccg ccccggatcg tttccagccc
     1981 ggccggagcg aaaccgaaat cttccgggct tacctggtaa ttggaaacgg cgccttcctt
     2041 caggtgggca ataaaggtcc ggccgacgat gctgatctcg tcgaaactcc cctccccgta
     2101 aaccaccagg gcgcttcggc agcccaggtt gcgcaggacc tcggccatca gcgtggtcaa
     2161 ctcccggctg tagacgccca tcacctgaac gttggcgccg gccggatttg agaggggacc
     2221 gagcaggttg aagatggtcc ggatgcccaa ctggactcga accggcaggg caaacttcat
     2281 ggccgagtga tagagcggcg cgtagaaaaa accgatgccg accttttcga cgcactgctt
     2341 gacctcggcc ggctccaggt cgagattgat gcccagggaa cgcaccacgt cggcgcttcc
     2401 gcaggaactg gagacggacc ggttgccgtg cttggcgacc aggcagccgg cgccggcaac
     2461 cacgaaagcg gtggccgtgg aaacgttaaa ggtgttggtg ccgtctccgc cggtcccgca
     2521 ggtgtcaatg atcgtctccc ggtcgacgtt gatatcgtca cggtcaaggt cgatcacctc
     2581 gcggtcgatg tcgatgcgga gggccttctg ccgcatcacc cgggcgcagc cggtaatttc
     2641 gtcgctggtc tcgcccttga gacgcagccc catcagaaaa gcggcgatct gggcctcggt
     2701 ggcccgcccc tccatgatca gggtcatgcc ccgggccgcc tcctcctggc tgagatcttc
     2761 tttccgcacc agacgggcaa tcaggtcttt catgagaatt ccttttttaa gcgcggcttc
     2821 aaacccgcag aaagttagcc agcagttttg agccttcccc ggtcaggatc gattccgggt
     2881 gaaattggac cccgaaaacc tggaaatttt catgctggag tcccatgatc aggccggaat
     2941 cggtccaggc ggtcacctgg agcgaagccg gcaggctctt ccgatccacc accagtgaat
     3001 gataccgggt cgcttcaaaa ggatcgggaa gccccagaaa aaccccctgg ccccggtgcc
     3061 ttatcatgga aaccttgccg tgcatcaggg tcggggcccg gatgatccgg cctccgaaag
     3121 cgtacccgat ggcctggtgg ccgaggcaga cgccgagaat cggcagcttg ccggcaaaac
     3181 gccggatcac ttccaccgaa atgccggcat cttcgggccg tcccggaccg ggggagatga
     3241 taatccggtc cggacgcagg cgggaaatgg cggcggactc gatccggtcg ttgcgaacca
     3301 ccttcacccg ggcgcccaat tccgaaagcg cctgaaaaat attataggtg aacgaatcgt
     3361 aattgtcgat caagagggtc atggccagat tcggttccgc cggccaaacg gccgcgaaaa
     3421 aaccgcctta ttttagcaca gctggccggt taagaccagc cggagcgatt ccagcaacgc
     3481 ccgccccttg ttcatggtct cctcgtattc ccggcccgga tcggagtcgg ccacgatgcc
     3541 ggcgccgact tgcaggtaag cccggccgcc cttgaccagg acggtccgga tggtaataca
     3601 ggtatccaga ttcccggcaa aatcaaggta cccgaccgcg cccgcatagg ggccccgcct
     3661 tttctgctcc agctcatcaa taatctccat ggcccgcacc ttgggtgccc cgctgacggt
     3721 gccggccggg aagcaggcct gaaaaagatc gagcgcgtcc cgccccggta ccagccggcc
     3781 ggtaatattg ctgaccagat gcatgacgtg ggagtaacgt tccacgctca tcaattcggt
     3841 taccttgacg ctgcccggcc ggcagacccg gcccaggtcg ttgcgcccca ggtcaaccag
     3901 catgatatgt tcggcctgct cctttcggct ccgccgcagc tccctttcca gggccaggtc
     3961 cgaaacttca tcggcgcccc tcggcctggt cccggcaatc ggacgcaggg tggcccggtc
     4021 ggcttccagg cgcaccatca cctccggaga ggaaccggaa atggagaagg ggccgaaatt
     4081 caggtggaag agatacgggg aagggttgat ggatcggatc gcccggtaaa gattgaactg
     4141 gttcgctccc gagcccggaa aatcgatggc cagacgctgg gaaagcacca cctggatggc
     4201 gtcgccgcga cggatgtaac ttttggcgcg ccgaacggcc gcctcgaaat cctttcgatc
     4261 ccaatcgggc ttcatccgga accggtccga agcagacgca gggccgcccg gatagggagt
     4321 cccggttttc cgcagccgac cggcgatact ttccagcctg aggacggctt tccggtaggc
     4381 gttttcaacg tccgaatcga tttcggcatg cgtaatcacg cgcagccggt ggcgaaaatg
     4441 atcgaaggcc agcagggtat cggccaggaa aaactggaaa tccggcattc ccagatcatc
     4501 gggattccgg tccggaatcc tctcgaaacg acggaccagg tcgtaggagc agtatccaac
     4561 cagccccccc gaaaaaggag gcagcccggg gtcgggagcc acccgaaacc cggacatcag
     4621 gtcacgcagg aagagcagcg gttcgaccga gcgggtctcg actctcccgc gccgatccag
     4681 gctgacccgg tcgccaacgg cgcttatgat aaggctggca tcggtgccca ggaaggaata
     4741 acgcccctgt ttttcgcccc cttcgacgct ctccagcaaa aaacccggac gtgccccgac
     4801 caccttccaa taggccgaca ccggcgtctc caggtcagcc aggatctcga cgctcaacgg
     4861 caccaggtta taatcccggg ccaggcggcg gaactcgtca aaggaagggg ttatttccat
     4921 cgatcggcca ttggtcagat ccggcgttcg aggtggcgca gccaccgggc gtcgtcccgt
     4981 tccggaaaat cggtccggaa atgggcgccg cggctctcgg tgcgttccag cgcgccgcgg
     5041 gttatcaggc cggccagcca gagcatgttc tggatttccc agccggaagg atcggccacc
     5101 cgcgaggtca gggcgtaact ttgccagtta ttaatctttt cgagggccag ctccaggtca
     5161 aagccgttgc gttccaggcc aacctgacgc cacatgaggg tgttaaggga acggcgcaaa
     5221 tcgttgaaat caacctggaa atcgttccag cggcgggagt agcgcacccg cacccggctt
     5281 ctttctccgt tttcggcggc gaaacgaccg gcccgccgac cgaaaacaag acattccagc
     5341 agggagatgc tggccagccg gttggcaccg tgcaccccgg tggcggccgt ctcgccgcag
     5401 acaaaaaggt tattcagggt ggtgcgggcc tccagatcgg acttcactcc gcccatgtgg
     5461 taatgaacgg ccggccggac cggaatctct tctttttttt tttcaagcag aagacggcat
     5521 acgatattgt aaatggtcgg gaaccgcttg cggaaaatgg aggcgaccgg acggcagtcc
     5581 aggtagacgt tgccgatgcc gcgtcggcgc atctggtcaa ggatggcccg ggtgaccacg
     5641 tcgcgcggcg ccaggtcggc cgccggatgg taatggggca tgaatctttc ctggcggtcg
     5701 ttgatcaggt aggcgccctc ccctcgcacc gcttccgaga tcaggaaacg gggcgcgccg
     5761 gcgatgtaaa gaacggtggg gtgaaattga atgaattcaa ggtccgtcag gaccgccccg
     5821 gcccggtagg cggccgccat cccgtcgccg gtggaaatcg aaggattggt ggtctcctgg
     5881 taaagctggc cgacgccgcc ggtcgccagg atggtggagg cggcccgaat aaccagcagg
     5941 gcgcgtttct cccgttcata aaaaagtcct ccgaccacgt tgtggccgga ggcaagcagg
     6001 tcgatcagaa agtagcgttc ccgaatctcg atctcccggg tctggagcac ccgggaggaa
     6061 agggtacgga ccaattccgc cccgctgcgg tctccggcgg cgtaaattat ccgcgcggcc
     6121 gagtggccgc cctcccgggc gaaggccagc tcccgtccct tgcggttaaa ggaagcgccg
     6181 aattccagga gttcggcgat gcattccggc ccctcctcca ccaggagccg ggccgccgcc
     6241 tccgaaacca ggccggcgcc ggcagcaatc gtatccttga ggtggttttc aaaggagtcg
     6301 cttgacgaca gaacggcggc cacccctccc tgcgccgttt ccgaggcgct gtccagcagg
     6361 ccgctcttca tggccagggt gaccccccgg cggccggcgg ccgaaagcgc ggcactcaaa
     6421 ccggccacgc cgccgccgat caccaggacc ccggtctcct cctcgggcag ggagagggtg
     6481 tcaaaatcaa gcaacccggc cggaatcatg ttaattccgg gtgaacttca agaccgaggc
     6541 cgacgcattc tggggatctt tgacccaggc tacatggccg tcggcaaaga aaaagttggc
     6601 ccccccgtca tgccgatcgg tgcagatatc gtaaaagaag ccgctgtcgg tcggagtgcc
     6661 ggagaaggtc gaaccggtct ggtcaaagaa gatcgcaaag gatgatgatt cagccacatc
     6721 ggcgacgctg taaccggcgg cgttggcgtt gtacccgtag ttcacgttgt tgtcccgatc
     6781 ggaaccgacg gctgacggac agaggtagct gccctcggcc aggtacccgc ttatctcgtc
     6841 ccaccaggcc ccggcactgg gcatcctctc atcattttca cgcgcgtaca tgaagagcga
     6901 catccccagt tgtttcaggt tgttgatgca ggtggtccgc cgggcctgtt ccctggcccg
     6961 ggataatgcc ggaagaatca tgccggccag aatggcgata atagcgatga caaccagaag
     7021 ttcaatcaag gtaaaacctt ttttgccgtt gatccgcatt aaaatcaccc cctttctcaa
     7081 ataatggcgc gatgatgaaa aacaccttgc aacacctgct tttattttac ctttaccgcg
     7141 gcccggtgtc aaaagaagcg accgggcagc cgtcgctgga aaccggcgag aaaatcatcc
     7201 agaatccgct gccggctgca gcgaactccg acggtttccc ggagtgaaat ggccttgaaa
     7261 ttccccgccc ggctttgaat ggaacagttg acattcaagc cgatacccag gacaacccgg
     7321 tcgccgcgcc tttccagaag cagtccggcc agcttgcgac tctccagata aaggtcgttg
     7381 ggcagtttaa ccgcgggccg ccggccggtc agtctgaaga ccgaatcggc cagctggtag
     7441 gcggccgcca gcagaaggcc gccggcctga acggaaggaa ccggataaag aaggattgaa
     7501 aaccagaggc cgccgggcgg cgattgccag tcacggccga ggcggccccg accggccgtt
     7561 tgccggaagg ccagcaccag gctgccggaa gggggcggat cggtttccag ccaccgccag
     7621 gcctcgtcct gagtggaatc aagccggccg taaaggcgcc agccgacacc gcctcgagaa
     7681 atactccgga agcccataag gcaaaagacg ggccgtcgat ccccgacgga ccggccgcta
     7741 aaagaccagc tggaaaacgg tcttggcgat cagggcgatc gccaccgaag ccgccccggc
     7801 cagaccgatg ccgcagccgt agccggcggt cagcagcggt gtcatgttgc gccagccggg
     7861 cccgatgcgt cgggcaaaga agtagcgtcc gatcatcccg cccaggaaca tgggcacggc
     7921 gttgtgggga agcataggga tgccgcccag aataccgtaa aacaaggcca tcggccattt
     7981 gaccaggctg aagattccgg ccaggccgag actggtcagg aaggtccacc cgatgaccgc
     8041 cttcttaaga ccgccgagca gaaaactgcg gccgaccgaa ctttcgacgg tcgaggatat
     8101 ccagagcgcc tgcatcatgg cctgctgggg ccacatccgc tgaacgtagg gatagatggt
     8161 cgagggaatc ggcgccatgc gccagatgat tgaccagaag gtaaagctgc agaagagcat
     8221 gatgccgagg gtcatggccg ccgtcttgta ccaggaggta aagcggatct tcaggatttc
     8281 catctgcttg aacccctggg cgatcccgcc atggttgaaa tagggcagcg gagcgtacca
     8341 gatggccgcg ccgcggtaac cggacaggat ataggtaccc tcgatcaggt aggggaagga
     8401 ggtctggtcc gagaccccgg ttatcccgta catccgggcc gaaatatagg tcagggccgg
     8461 agtcagcagg aacccgaagg agaccggaat ccagatcggg aagtccggga ccaggtaacg
     8521 gaccagcagg acataggcga gcgtcgcgaa gaaccagagg ccgatggcga tccagatcgg
     8581 atagtcgccc cggtcggcga cgacggccgg agcggccgtc ttttcaagat gacccttccc
     8641 gcgggcgctc cgcagccgga taaagatgga aatgatgctg atcacgaaga ccgccaggcc
     8701 ggtgccgatg gtcacgctca cccagaaatc gatggtggtg gaaaccgagg ccggaatggc
     8761 gcccatgccc ggctgccagg tcttcaggaa atcggtatgc ttgtagatca ggggattggc
     8821 aattgtcttg gcgcccaccg aaccgatgaa gtggcccacc accacccaga aaggcagcac
     8881 aaaaccaatc aggaaactgc ccaggtcggt gaccatcccg aagatggccg ccgggaatcg
     8941 ggaacccagc tgcgaggtga aatccacgaa gggaatcggt atgagcatga agggcttgga
     9001 catggtcaag ccggtgatgg tcgggataaa gacgtaaaag gccccgtaga tgaccccgag
     9061 catggcgccg atggtaaaga tccgccagcg ctgggtatcc ttcttggcgg ccaggtcggc
     9121 cagggccagc acgccctggg tctgaaccgg ggccatcggg aactcgagcc gctcgaaatc
     9181 gctggtaacc cggaagaaaa tgtacccgag cgtcagggaa ttaagaagat gaagggtgct
     9241 ggtggtcacc aggatggcaa tgggtttgac ccagtccgag tggaagaagg tgcgctgaag
     9301 cagggctgga ctgccggccg gcggcaccgc gaaacgcggg atcaggtcgg ttaacccgaa
     9361 ggccttggcg tagggagact gaatgaagta ctggttgtac atcaccgggc cgaaggcgcc
     9421 gcccccggcc agggcggccg tggccagcag gattatgtat atctcctggc gcttgagcgt
     9481 tatgaacgac cgcttggcga tttcgatgaa cagaatcagc gtgacccact gggcggccgg
     9541 ccccaggctg gtgccggcaa tgagccccat gtaaatggcc gccggcatca tgatgaagcc
     9601 gacaaaaagg gccgcaatgg gcgccttggc gttaaatccg ttctcgaagc gttccggagt
     9661 caactggttt tgattttcca tggaaggtta tttcccgtta gcgatgacct tttcaccctg
     9721 ctggcgatac tcgtccttga cctcgggcgg cagggttcgc cagaccagga actgtttccg
     9781 gatactgtcc aggaaccgcc ggttcaaacg ctgccagtcg ccgcgttcgc cattctcgcg
     9841 tcgaatctcg acctggatcc cgtaaaagac gaattcgctc atcgggtagg cgcgcaactg
     9901 gaccgtctgg ctgacgccca ggtcgaaggg ggccagggaa acgttgcacc cggtgatgta
     9961 agcggacccg cgctcggtct tttcggcgtt gaaccgggcg tcgctggtgt aaaagctgcc
    10021 ggtggcctcg atcgaatacg aggcaaagta atcgcgcagg aaggtcatca aacccaggac
    10081 ctcggtctgg ccgacggtaa aaggaaactc gaagagccag gtatccccgg tcggacgcgg
    10141 cagcacccag cgccgggtaa cgtccggaac cgagagctgg gacgccttgc gggc
//
LOCUS       DAOQYN010000045        10153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_7075_length_10153_cov_4.601406, whole genome shotgun sequence.
ACCESSION   DAOQYN010000045 DAOQYN010000000
VERSION     DAOQYN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10153
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7075_length_10153_cov_4.601406"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2132
                     /locus_tag="PKN80_03015"
     CDS             <1..2132
                     /locus_tag="PKN80_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNQ35016.1"
                     /translation="LGGLWVEPDCNLTGGESLVRQVIQGTRFWREEFGTSSPIAWLPD
                     AFGFNGALPQILKGAGIDYFFTVKLYWNVASRFPHTLFWWEGIDGTRVLAHVPYLKGG
                     YSGFPGAADLRRARSECRHAEVYPATLFPMGHGDGGGGVTPEMLETARRFRDFPGLPR
                     VRFVRAADFFKAAERRGAGLPVWRGELYYETHQGCYTSQAFIKRSNRRLEEALVELET
                     LRGLKALLSGRRPGSGLDEIWREVLTYQFHDILPGTSVPEAYAEIEPRCRKLLAGLKR
                     SLDSELGQLAPAAGRKSRRGRPAFLVFNTLNWPRREVVELEPGRPVELALPAGGYLVT
                     AGSGLKSGRPEDRPRVSPTRLENRFFRMELDRQGRLARLYDKVNRREVLAEGAAGNDF
                     RIFREAPVHYEAWDIDADYEVTGRSFGRLVRRRVLEAGPVRAAVRQEFSDGRSRLVQD
                     IIIYAGLPRIDFRTEVDWQERRRMIKAAFPLSLKAGEAWYEIPFGAIARPAVPAGPEG
                     AFRREVSGHHWADVSEAGYGVALLNDSKYGYDFDGQTLRLTLLRAPMYPNPDADRGRH
                     LFTYALYPHAGRWPEAGVVEQGFSLNRPPLVRSLAESAPESGPPVSLFRTEGVPVVVS
                     AFKAAEDGRGFVIRLYEPGGQSGRAKLFLPAAPVAAWETNLLEEERRPFQVDRETVEF
                     EVRPFEIKTFYFSFSKEPGEPDGRKKS"
     gene            2113..3069
                     /locus_tag="PKN80_03020"
     CDS             2113..3069
                     /locus_tag="PKN80_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35017.1"
                     /translation="MAEKKVDFSRIKTSTAAGRKNLVHLGNLMQPGRDQVAPVVNPEF
                     EELAERMRAAREAGAPILWSIGAHVVKCGLSRYLVEFMKAGYLSHLAGNGASSIHDFE
                     LACLGGTSEDVPTAIEDGSFGMWEETGRWMNEAIQAGWKRGLGYGRSLAAFVDEHPER
                     FPNREICLFYQAARLGLPATYHIALGTDIIHQHPIVDFSAIGGASGIDFHEYCETVAR
                     LENGVFLNFGSAVIGPEVFLKALSIARNLGFQVKKFTTANFDLIDLGDYRSKVADDVY
                     HYYYRPRKNIVNRPVSMGGKGFHFNLDHRVSIPGLYQRLLAG"
     gene            3089..4057
                     /gene="tsaD"
                     /locus_tag="PKN80_03025"
     CDS             3089..4057
                     /gene="tsaD"
                     /locus_tag="PKN80_03025"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR00329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNQ35018.1"
                     /translation="MLVLGIESSCDETAVALVADGRRILASEVASQAELHLPYGGVVP
                     ELASRRHLESLLPLYRLCLEKSGQPAAAIDLVAATVRPGLSGSLLVGESFGRALAAGL
                     ERPFLGIDHLEAHLSAGFPDGLPEFPALGLVVSGGHSSIFLVEDPDTFRLLGQTRDDA
                     AGEVFDKVARVMDLPYPGGPPIERLALQARHPVRFPKPGVRNSLDFSFSGLKTAVIYY
                     RQGHPEAAAADVAAGFQAAVAEGIAGKLDYALSRFPVKSVLTGGGVLANRFLRSRLET
                     WAQGRGVRLYVPPIERSLDNAVMVAVRAFYRRSAPPGSGARPPGNP"
     gene            complement(3993..4523)
                     /locus_tag="PKN80_03030"
     CDS             complement(3993..4523)
                     /locus_tag="PKN80_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNQ35019.1"
                     /translation="MESKEMLALAAEVGEGLRGKGLNLGVAESCTGGRLAALITAVPG
                     SSDYFLGGVVAYHNRVKRRALKIPAGRLRRFGAVSPETAAAMAGNIRKVTGGRIGLSI
                     TGIAGPAGGSPEKPVGLVYLGFSTRKVRKVFECRFPGTREEIRRQACLEALEILRDYL
                     ADGPRFQAAPNAGKRP"
     gene            4728..5831
                     /locus_tag="PKN80_03035"
     CDS             4728..5831
                     /locus_tag="PKN80_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35020.1"
                     /translation="MNRGRGFFAAVLLLGFLFNGLAQPPSASAQASPVGRITWPREAN
                     EFFLKCLAGEPAAGPLSFQFWTWDQAGQMRTSLRSHGPLPESLVFGPPRRQADNSVMV
                     ACLVYRLTTGNEKGIMGFELAFDCDAKTLGTLEELYRMRRLNCLVTFSRPEVEKSAGP
                     GGLPMFGFKGLKADLAFFDGRSEVFKTVARLDKFKDLPDPAVKLENQTPALELKAEFE
                     DLPGLKYFLDWEPEHPAWYAREFNTASGRGVAVCQEKAADKVWKTKLPRSLPPGRYSL
                     LFSSERMRHRIDQNVLRVTLNDCTREFVWLGTGGWVIGPVFETRSAGDRLTFEAVQLG
                     SVGLNHHPNYFNQRFILLDRFLITTDLNLKEVK"
     gene            5828..8611
                     /locus_tag="PKN80_03040"
     CDS             5828..8611
                     /locus_tag="PKN80_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35021.1"
                     /translation="MKTRRFLPVLAFCLVLLAGGTVRAANLVHNGSFELGVTKDSWAK
                     LFEDDMISSENPHHGKRCLSVKEISNRGISSAFYRLAPGDYRVSFAYRAPAGSSLVFA
                     LQNSKHQDLFRATLAAKTEWQLFSRDFKVTAGDDYALFYFGNNWALDAVCLAPAADNL
                     AGYAPPVPVEPAVRADVLANTFYSGQPVQLTVAAYNHGAAPAPVTLAYDVVDIAGRTV
                     YQGEFSLPAVPAGGYQEKKETLANHPNGLFSLVFKAKESGYTGEAVYAVLPEVKGVGP
                     LGMFTHSNNPELKYLNRVNVRWYHPLTDGIFRGYTITPNKGEYVWFDENVARINQYLP
                     KAVGLLCHYIYEPAWYAQEQVVAPRREYFVGRGAYGAESSRLKDEVWSELVYNIVSHY
                     RGKIDYWVIDDEFDSAVTAEEFGPVMKTAALAARKADPQAKVTCMPGTQNFFEELIDL
                     GYGDYFDVIPCAERHWWGLRKTAWLVRKYNKGVISYAHLRGNTFYRTHEKIPFSTSRA
                     AARGAYNQVARLVLAGHSETVAPYRTQFTDSVSMKSVFDRSMLDYDGTLKSNGIGFMM
                     AGSLLAGARPVDELMLNGKGMLLMGFLDGEGRPSVLAYGANELKLNYPAGRVRLFDWL
                     GNPVKGPADVGGGFALTLAEDPIYIKSTDPAGAAAFFKAVRAAYEAFEAPPAAFFGFT
                     RVENGQGIVYTLNPDGTATDSAAVPLPPNRSLGYQPYRRARLWLLNAYPAPKNLALDG
                     DLAEYDALPAGDGVFTIWGWPGGQVQSRSDDQVIEGGEFVSYNNNRDFKAYLNSCWDG
                     ERLAFAFRFNDEKVIPGRDGLEIHLWSDWPAGAFGKPVKLLVKAGEERGRLVKADGSE
                     ITIPARFKKTAAGYQVEVAAAPAALGLSAAAPGRVIGLDFYFDDCDSEDGKETTRYRW
                     AAGSGGPGQLLFK"
     gene            complement(8699..10072)
                     /locus_tag="PKN80_03045"
     CDS             complement(8699..10072)
                     /locus_tag="PKN80_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35022.1"
                     /translation="MAEALPLAGESQLDVTGRRAAAFFSISVFRRGVGDFKGAYDDYV
                     KTIKYQVKNLHSEPDPKSRALLGADLFVKTLAEVGDIEGALRLRDQLENGYERKNSTE
                     KICFVLLFAGNPKKSMEIAAENDIRFDAFDCHWFAEAFAREGDYANARIFAERAGRES
                     TYLFIAESQLSRGDIAEAVRYARLAGDDAVGMAIRGEPEAAFQSLKNSKPGVVSPYLY
                     IKDLNRIIMNLIRLGDMGNARKAVGLAPELIKQKSRNDRFASGLALLGNLYLDVETGK
                     TDEILKTMDRGLLLTNLYQDSREKERKGTEQEYAKERAEWLSELCSWAIERKQPAAAE
                     RIADSFAESRERIEFLRRVLALYDSKADRANTERLTKKLAGLLAEVQAGGWDPENPGR
                     EFVVNAWTDAAYALDAESALVNLPGRIAEIKKESSPDEMPVQIAICASEIGKGLLRIR
                     ALDRIYR"
BASE COUNT         1663 a         3399 c         3236 g         1855 t
ORIGIN      
        1 cgctgggcgg cctgtgggtg gagccggact gcaacctgac cgggggcgaa tcgctggtgc
       61 ggcaggttat ccagggtacc cgcttctgga gggaggagtt cgggacctcc agcccgatcg
      121 cctggctgcc ggacgccttc ggtttcaacg gcgccctgcc ccagatcctg aagggggccg
      181 ggatcgatta ctttttcacc gtgaagcttt actggaacgt ggccagccgc tttccgcaca
      241 ccctcttctg gtgggagggg atcgacggta cccgggtcct ggcccacgtt ccctacctca
      301 agggcggcta cagcggcttc cccggcgcgg ccgacctgcg ccgggcccgg tccgaatgcc
      361 gccacgccga agtttatccg gccaccctct ttccaatggg ccacggcgac ggcggcggcg
      421 gcgtcacccc ggagatgctg gagacggccc gccgtttcag ggattttccc gggctgcccc
      481 gggtccgctt cgtccgggcg gccgacttct tcaaggcggc cgagcggcgg ggcgccggcc
      541 tgccggtctg gcgcggcgaa ctctactacg agacccacca gggttgctac acctcccagg
      601 ccttcatcaa gcggtccaac cgtcgcctgg aggaggccct ggtcgaactc gaaacgctcc
      661 gcggtttaaa ggcgctcctt tccggccgcc ggcccgggtc cgggctggac gagatctggc
      721 gcgaggtgct cacctaccag tttcacgaca tcctgcccgg cacctcggtg ccggaggcct
      781 acgccgagat agagccgcgc tgccggaagc tcctggccgg tctgaagcgt tctctcgatt
      841 ccgaactggg acagctggcc ccggccgccg gccgcaagag ccggcggggc cggccggcct
      901 tcctggtctt caataccctc aactggccgc gccgggaggt cgtggaactg gaaccgggcc
      961 ggccggtgga actggccctg ccggccggcg ggtacctggt taccgccggg tccggcctca
     1021 agtcgggccg gccggaagat cggccccggg tcagtccgac ccgcctggag aaccggttct
     1081 tccggatgga actggaccgg caggggcgcc tggcccgcct ttacgacaag gtcaatcgcc
     1141 gcgaggtcct ggccgaaggc gccgccggca acgatttccg gatcttccgg gaggccccgg
     1201 tccattacga ggcctgggat atcgacgccg attacgaggt caccggccgc tccttcggac
     1261 ggctggtccg gcgccgggtg ctggaagccg gcccggtccg ggccgcggtg cgccaggagt
     1321 tcagcgacgg ccggagtcgg ctggttcagg atatcatcat ttacgccggc ctgccccgga
     1381 tcgatttccg gaccgaggtc gactggcagg agcgccgccg gatgatcaag gcggccttcc
     1441 cgctctccct gaaggccggc gaggcctggt atgagatacc cttcggcgcc atcgcccggc
     1501 cggcggtccc ggccggcccg gagggggcct tccgccggga ggtctccggc caccactggg
     1561 ccgacgtctc cgaggccggc tacggggtgg ccctgcttaa cgattccaag tacggctatg
     1621 attttgacgg gcagaccctg cgcctgaccc tgctgcgggc gccgatgtac ccgaacccgg
     1681 acgccgaccg cggccgccat ctcttcacct acgcgcttta cccccacgcc ggccgctggc
     1741 cggaggccgg cgtggtcgaa cagggcttct ccctcaaccg gccgccgctg gtccggtccc
     1801 tggcggaatc cgctccggag tccggcccgc cggtctcctt attccggacc gagggggtgc
     1861 cggtggtcgt ctcggccttc aaggcggccg aggacggccg gggttttgtc atccgccttt
     1921 acgaaccggg cgggcagtcc ggccgggcca aactcttcct gccggcggcg ccggtcgcgg
     1981 cctgggagac caacctgctg gaggaggagc gccgcccgtt tcaggtcgac cgggagaccg
     2041 tcgaattcga ggtccgaccc ttcgagatca agaccttcta cttttctttt tcgaaagaac
     2101 cgggagaacc cgatggccga aaaaaaagtt gacttcagcc ggatcaaaac cagcaccgcc
     2161 gccggccgca agaacctggt ccacctcggc aacctcatgc agccgggacg ggaccaggtt
     2221 gcgccggtgg tcaacccgga attcgaggaa ctggccgagc ggatgcgggc ggcccgagag
     2281 gccggcgcgc ccatcctctg gtcgatcggg gcccacgtgg tcaagtgcgg cctctcccgc
     2341 tacctggtcg agttcatgaa ggccggctac ctgagccacc tggccggcaa cggcgcctcc
     2401 agcatccacg atttcgaact ggcctgcctg ggcgggacct ccgaggacgt gcccaccgcc
     2461 atcgaggacg gctccttcgg catgtgggag gagaccggcc gctggatgaa cgaggcgatc
     2521 caggccggct ggaagcgggg cctgggttac ggccggagcc tggccgcctt cgtcgacgaa
     2581 cacccggagc gatttcccaa ccgggagatc tgcctctttt accaggccgc ccggctcggc
     2641 ctgccggcca cctaccacat cgcgctgggg accgacatca tccaccagca cccgatcgtc
     2701 gatttttcgg ccatcggcgg cgcctccggg atcgattttc acgagtattg cgaaacggtg
     2761 gcccgcctgg agaacggcgt cttcctgaac ttcggcagtg ccgtcatcgg cccggaggtc
     2821 ttcctgaagg ccctctccat agcccgcaat ctcggcttcc aggtgaagaa gttcaccacc
     2881 gccaacttcg acctgatcga cctgggcgac taccggagca aggtggccga cgacgtctac
     2941 cactactact accggccgcg caagaacatc gtcaaccggc cggtgagcat gggcggcaag
     3001 ggttttcact tcaacctcga ccaccgggtt tcgatacccg gcctttacca gcgtctgctg
     3061 gccggctgag cggaacccgg agccgaagat gctggtgctg ggtatcgaat cctcctgcga
     3121 cgagaccgcg gtcgccctgg tggccgacgg ccgccggatc ctggcttccg aggtggccag
     3181 ccaggcggag ctccaccttc cttacggcgg cgtggtcccg gaactggcca gccgccgcca
     3241 cctggagagt ctcctgcccc tttaccgcct ctgcctggag aagtccggcc aaccggccgc
     3301 cgcgatcgac ctggtggcgg ccacggtccg gcctggcctc tccgggtcac tgctggtggg
     3361 cgagtccttc ggccgggccc tggccgccgg cctggagcgc cccttcctgg ggatcgatca
     3421 cctggaggcc cacctttcag ccggtttccc ggacggcctg ccggagttcc cggccctggg
     3481 gctggtcgtc tccggcggcc actcctctat cttcctggtg gaagatccgg acaccttccg
     3541 tctcctgggc cagacccggg atgacgccgc cggagaggtc ttcgacaagg tggcccgggt
     3601 catggacctt ccctatcccg gcggcccgcc gatcgagcgg ctggcccttc aagcccgcca
     3661 cccggtccgg tttccgaagc cgggcgtgcg caactcgctg gacttcagtt tcagcggcct
     3721 gaagacggcg gtgatctact accggcaggg gcacccggag gcggcggcgg ccgacgtggc
     3781 cgccggtttc caggcggccg tggccgaggg gattgcgggc aaacttgatt acgcgctgag
     3841 ccgcttcccg gtcaagtcgg tgctgaccgg cggcggggtg ctggccaacc ggtttctccg
     3901 gtcgcggctg gagacctggg cgcagggtag gggagtgcgg ctttacgtgc cgccgatcga
     3961 gcgttcgctc gacaacgcgg tgatggtggc ggtcagggcc ttttaccggc gttcggcgcc
     4021 gcctggaagc ggggcccgtc cgccaggtaa tccctgagga tctcgagggc ttcgaggcag
     4081 gcctgccggc ggatctcctc ccgggtaccg ggaaaccggc actcgaagac cttccggact
     4141 ttacgggtgc tgaatcccag gtaaaccagg ccgaccggct tctccggcga gccgccggcc
     4201 ggtccggcga tgccggtgat ggacaggccg atccggccgc cggtgacctt gcggatgttt
     4261 ccggccatgg cggcggccgt ctccgggctc acggctccga agcggcgcag ccggccggcc
     4321 gggatcttga gggcccgccg cttcacccgg ttgtggtagg cgaccacccc gcccaggaag
     4381 taatcggaac ttcccggcac cgcggtgatc agcgcggcca gccggccgcc ggtgcaggat
     4441 tcggccactc cgagattcag tccttttccc cgcagccctt caccgacttc ggcggccagt
     4501 gccagcatct ccttcgattc catgcttaaa attataaggc aaaaggcggg cgtccgtaaa
     4561 acgccttttt caatcagcgg ccggccgggt ttgacaggta cctcgccttt attatagttt
     4621 attgcaggat tgtccacggt tttcacaagc taatcaaacc gggattaacc gggtttttcc
     4681 cgggttgccg ggttatcatc cggcctgcga agtcaacagg ggggcagatg aacagagggc
     4741 gcggtttctt tgcggcggtt ttgctgctgg gctttctttt caacgggctg gcccagccgc
     4801 cgtcggcctc ggcccaggcc agtccggtcg gccggatcac ctggccgcgg gaggccaacg
     4861 agttcttttt gaaatgcctg gccggggaac ccgcggccgg ccccctttcc ttccagttct
     4921 ggacctggga ccaggccggc cagatgcgga ccagcctccg ctcgcatggc ccgctgcccg
     4981 agagcctggt ctttggcccg ccccgccgtc aggccgacaa ttcggtgatg gtcgcctgcc
     5041 tggtctaccg cttgacgacc ggaaacgaaa agggcattat gggcttcgaa ctggccttcg
     5101 actgcgacgc caaaactctg ggaacgctgg aggaactcta ccggatgcgc cggcttaatt
     5161 gcctggtgac cttcagccgg ccggaggtgg agaagagcgc cggtccgggc ggcctgccga
     5221 tgttcggctt caagggattg aaggccgacc tcgccttctt tgacggccgc agcgaggttt
     5281 tcaagaccgt cgcccggctc gacaagttca aggacctgcc ggaccccgcc gtcaagctcg
     5341 agaaccagac gccggccctg gaattgaagg cggagttcga agacctgccc ggtctcaagt
     5401 acttcctcga ctgggaaccg gagcatccgg cctggtatgc ccgggagttc aacaccgcct
     5461 ccggccgcgg cgtggccgtc tgccaggaga aggcggccga caaggtctgg aagacgaaac
     5521 tgccccggag ccttccgccc ggccggtaca gcctgctctt ctcttccgag cggatgcgcc
     5581 accgcatcga ccagaacgtc ctccgggtga ccctgaatga ctgcacccgg gagttcgtct
     5641 ggctgggaac cggcggctgg gtgatcggtc cggtcttcga gaccaggagc gccggcgatc
     5701 gcctgacctt cgaggcggtc cagctgggtt cggtcggcct caaccaccat cctaattact
     5761 tcaaccagcg gttcattctc ctagaccggt tcttgatcac caccgacctg aacctcaagg
     5821 aggtcaaatg aagacgcggc ggtttctccc ggtcctggca ttctgccttg ttctgctggc
     5881 cggcggaacg gtccgggcgg ccaacctggt ccacaacggc tccttcgaac tcggggtgac
     5941 caaggacagc tgggcgaaac tcttcgaaga cgatatgatc tcctccgaaa atccacacca
     6001 cgggaagcgt tgcctgagcg tgaaggagat cagcaaccgc ggcatttcca gcgcctttta
     6061 ccggctggcc ccgggcgact accgggtctc ctttgcctac cgtgcgccgg ccgggagcag
     6121 cctggtcttc gccctccaga acagcaagca ccaggacctc ttccgggcca cgctggccgc
     6181 caaaacggaa tggcaactct tcagccggga cttcaaggtg accgccggcg acgattacgc
     6241 cctcttctac ttcgggaaca actgggcact ggacgcggtc tgcctggcgc cggccgccga
     6301 taacctggcc gggtacgcgc cgccggtccc ggtcgagccg gcggtgcggg ccgacgtgct
     6361 ggccaacacc ttctacagcg gccagccggt gcaacttacc gtggccgcct ataaccacgg
     6421 cgccgctccg gcgccggtga ccctggccta cgacgtggtt gacatcgccg gtcggaccgt
     6481 ctaccagggc gagttttcac tgccggcggt gcccgccggc ggttatcaag agaagaagga
     6541 gaccctggcc aaccatccca acggcctctt ttcgctcgtc ttcaaggcga aggagagcgg
     6601 ttacaccggc gaggcggtct acgccgtcct gcccgaggtc aagggagtcg gtccgctggg
     6661 catgttcacc cattccaaca acccggaact caaatacctt aaccgggtca acgtgcgttg
     6721 gtaccacccg cttaccgacg gcatcttccg gggctacacc atcaccccca acaagggcga
     6781 gtacgtctgg ttcgacgaga acgtggcccg gatcaaccag tacctgccga aggccgtcgg
     6841 ccttctctgc cactacatct atgagccggc ctggtacgcc caggagcagg tggtcgcgcc
     6901 ccgccgggag tacttcgtcg gccgcggggc ttacggcgcc gagagcagcc gcctcaagga
     6961 cgaggtctgg agtgaactgg tttacaacat cgtcagccac taccggggca agatcgatta
     7021 ctgggtcatc gatgacgagt tcgactcggc cgtgacggcc gaggagttcg gcccggtgat
     7081 gaagacggcg gccctggccg cccgcaaggc cgacccccag gccaaagtca cctgcatgcc
     7141 gggtacccag aacttcttcg aggagctgat cgacctcggc tacggcgact acttcgacgt
     7201 cattccctgc gccgagcgcc actggtgggg attgcgcaag accgcctggc tggtcaggaa
     7261 atacaacaag ggggtcatca gttatgccca cctgcgcggc aacaccttct accgtaccca
     7321 cgaaaaaatt cccttttcga cctcgcgggc ggccgcccgg ggcgcttaca accaggtggc
     7381 ccggctggtc ctggccggcc actccgagac ggtggccccc taccggaccc aattcaccga
     7441 ctccgtctcc atgaagagtg tcttcgaccg cagcatgctg gattacgacg gcaccctgaa
     7501 gagcaacggc atcggtttca tgatggccgg gagcctgctg gccggggccc ggccggttga
     7561 cgagctgatg ctcaacggca aggggatgct gctgatgggc ttcctggacg gcgaggggcg
     7621 gccctcggtg ctggcctatg gcgccaacga actgaaattg aactacccgg ccggccgggt
     7681 gcgcctcttc gactggctgg gcaacccggt caagggtccg gccgatgtcg gcggcggttt
     7741 cgccttgacc ctggctgaag acccgatcta catcaagagc accgacccgg ccggcgccgc
     7801 cgccttcttc aaggcagtcc gggccgccta cgaggccttc gaggcgccgc cggccgcctt
     7861 cttcggcttc acccgggtcg agaacgggca ggggatcgtt tacaccctga acccggacgg
     7921 gaccgccacc gactcggcgg cggtaccgct gcctccgaac cgttcgctcg gataccagcc
     7981 ctaccggcgg gctcgcctct ggctgctgaa cgcctacccg gcgccgaaga acctggccct
     8041 ggacggcgac ctggccgagt acgacgcgct gccggccggc gacggcgtct ttaccatctg
     8101 gggctggccg ggcggccagg tccagtcgcg cagcgacgac caggtgatcg aggggggcga
     8161 gttcgtctcc tacaacaaca accgggattt caaggcctac ctgaacagct gctgggatgg
     8221 ggagcgcctg gcctttgctt tccggttcaa cgacgagaag gtgatccccg gccgcgacgg
     8281 cctggagatc catctctggt ccgactggcc ggccggcgct tttggaaagc cggtcaagct
     8341 gctggtcaag gccggggaag agcgcggccg gctggtcaag gccgacggga gcgaaattac
     8401 gattccggcc cggttcaaga agacggcggc cggataccag gtcgaggtgg cggcggcgcc
     8461 ggcggcgctc ggcctgtcgg ccgcggctcc gggccgggtg atcggtctgg atttctactt
     8521 tgacgactgc gattccgaag acggcaagga aaccacccgt taccgctggg cggccggctc
     8581 gggcggcccg ggccagctgc tcttcaagta gtccggtctt tgttgtgaaa ggtgggcggc
     8641 cccggatccc ttttccggtg gcccggccgg cgggaatccg aggaggaaac ggaggttttt
     8701 accggtagat ccggtccagc gcccggatcc gcagaagtcc cttgccgatc tcgctggcgc
     8761 aaatcgcgat ttggacgggc atctcatccg gggatgactc ttttttgatt tcggcgatgc
     8821 ggcccggcag gttgaccagg gccgattctg cgtccagcgc ataggccgcg tccgtccagg
     8881 cgttgaccac gaactcgcgg ccggggtttt ccggatccca gccgccggcc tgcacctcgg
     8941 ccagcaggcc ggccagtttt ttggtcaggc gctccgtatt ggcgcggtcg gccttcgaat
     9001 cgtaaagcgc cagcacccgc cttaagaact cgatccgctc ccggctctcg gcgaaggaat
     9061 cggcgatcct ttcggccgcc gccggttgtt ttcgttcaat tgcccaacta cagagttcgc
     9121 ttaaccattc agcgcgttct tttgcgtatt cttgttcggt tccctttctt tctttttccc
     9181 gtgaatcctg gtaaaggttc gtcagaagca atcccctgtc catcgtcttg agaatttcat
     9241 cagtttttcc ggtttccaca tccagataaa gattgccaag cagtgcaagc ccggaagcaa
     9301 accggtcatt ccgggatttt tgtttgatca gttccggagc gagcccaacc gccttccggg
     9361 cgtttcccat atcgcccagg cgaatcagat tcatgatgat ccggttgaga tctttgatgt
     9421 agagataagg agaaacaacg cctggtttgg agttttttaa actttgaaaa gccgcctccg
     9481 gttcgcctcg gatggccatg ccgacagcgt catcgccggc cagccgggca taccggacgg
     9541 cctccgcgat gtcgccgcgt gacaactgtg attcggcgat gaagaggtag gtgctttccc
     9601 gcccggccct ttccgcgaaa atccgggcat ttgcataatc gccttcccgg gcgaaggcct
     9661 cagcaaacca gtgacaatca aacgcatcaa aacggatatc attttccgcg gctatttcca
     9721 tcgatttttt cgggtttccg gcaaagagca gcacgaagca aattttttct gtcgaatttt
     9781 tgcgttcata accattttcc agttgatcgc gaagtcgcag tgccccctct atgtctccca
     9841 cttcggccag ggtcttcacg aacaggtcgg ctcccaagag agcccgcgat ttcgggtccg
     9901 gttctgaatg caaattcttg acctggtatt ttattgtttt gacgtaatcg tcataggcac
     9961 ccttgaaatc accgacgcct cttctgaaga cgctgatgct gaaaaaggcc gcggcccgcc
    10021 taccggtaac atccagctgt gattcccccg ccagcgggag tgcttcggcc aagtcctcgg
    10081 cctgcttgaa gagccgccgg accttttcat cggccgcctg ttccagccgg gcgatctcgg
    10141 cccggacctg gtc
//
LOCUS       DAOQYN010000046        10090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_7143_length_10090_cov_5.254908, whole genome shotgun sequence.
ACCESSION   DAOQYN010000046 DAOQYN010000000
VERSION     DAOQYN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7143_length_10090_cov_5.254908"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="PKN80_03050"
     CDS             <1..1409
                     /locus_tag="PKN80_03050"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNQ35023.1"
                     /translation="GADRFLLYSAVPVVLGPNSLRSTNIYDSDGSLIALVPAYAAIGR
                     LMAGATGGRRLPVPDENLTAFAFERGGESHLIFWVDPGLGAYGNQQAGQYRLEVALEG
                     MVTDIFGNPYPVEKLGGGRTGIEFQGQPLLLNGGRAGLEAIARSLESAVFQNYLPVRG
                     YCRLDESGRPVLRLVMENRTRQPFAGELKVEEERGLLAEGTSFTRTLDLAPGRRATFD
                     LACQPSLLGRRPLANGVAEVSMGSRFNAGFGIWAQPAFRLAEVPTPDGDPAGWPGAPA
                     YIYSTVRASGGYERSQVREGAEFITREKTLQTAIKAGWDENNLYLAIRNVDDELNFAR
                     QASEAGRGDRVVIGLDTRLEADIFDIRRNEDDFLIQLDAVGEQGRARLIDWQGKAVEL
                     PVNLKRVVAGADSGYLFLVSCPWERLGVKPTAGAVLGFDVTLFETDKDRAPRIEAAWA
                     GFGFNLGNPTGYGQLILK"
     gene            1450..3201
                     /locus_tag="PKN80_03055"
     CDS             1450..3201
                     /locus_tag="PKN80_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ35024.1"
                     /translation="MFGRLRRPAGFLLLAVLLWCRPLAAEEPLFRFCQISSPTIAAAP
                     ADLAGDRSHYGRNVEMLKQRVGVVNGLKPAFLLVDGTVTAVGSAADYRLLAGILGEVK
                     APVYLGKGDDRGRAGRIPDPARVAPPADFEKEKIFDYRPFFSTDLKGIHLAFIMEGYG
                     TPAAAEFLESDLAAAEKRGARGLIVLLNTTEVYSFVAWWQSRGKVPVFPDEKLAALFK
                     KYQVSLVVLPGVPGQTQFPFLRAGDTYYVSGQGLAIDTRFYPSQGLIYEVYPDRTLVR
                     TIPLDGQPSLELTAWNGRAPGGRAARDRYLDNPYGCESYAEDLAAKPLLTFVQISDTQ
                     HSPKRWDSYRRRVDVLAYEAAVEDINRLRPAFVINSGDLVDYSTPEAYAEYQRVSNLL
                     AVPLYETPGNHDRYLPEGQAWPEGTTLEAKLAQYLKHTRPNGLDYAFEKNGVRFISVN
                     STEDRVNDLEFLKKALEESGKSRYLFVYTHFPFSEVFGNGVANADEAAPLLAKYQPTA
                     LLGGHRHRFAWRVIDGIPNFLADGIAWGSGMARADLVGGRVISVYHLYPDRLVVGMKP
                     LYSSIYARITVPVKARR"
     gene            3205..6645
                     /locus_tag="PKN80_03060"
     CDS             3205..6645
                     /locus_tag="PKN80_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35025.1"
                     /translation="MASGLECRLEARRVERTTPLSAAPGFFIAAGLNGRAAARRLAGC
                     LERHQRTIRILEDPPLAALEEAGSGLLIGNLADSRAVRDLYYRFLLVTDRGYPGPGGY
                     EIRTLLDPFGRGHNFVHLGYSDRAGLEAGLALLTERLADPLPYFSEVKFSRLPVPDAA
                     AERLRRTPLPADPRFIVHDEFGDLKGYLGYLSGEPGLLADFHRVLAATIEAGRIGHLY
                     AYSRLVVWRLLEYQGLIPEELRQPFLETVYQWAEGHEGIGRLLENPGYTSPHLPRQNH
                     GLMPALALLYAADYFQVHHPGLAGPAWWRQAAETLFQPYASSWKHHEDCVIHGFRLSQ
                     PMLLGRGLLDPAHRYFEAGGAARAAECAMVLVNNRGLTPNFGDSDLIRSFPGPSLRAA
                     AAYYRDGRYAYVNDLAADGQRRDGLELPVFQAFEAGVESKEPERPAGGLTVIPLDPLI
                     YRVWEREPELAVGFTAAPPAAPIDECFDKLCIRTGWRENDDYLLVDGLGGALHIFDRK
                     IDCTHAYDDAAAVLEYSRFGLAGLVSEDSLVLTDPEDHTAVTVTRDGRRGPVPAYAAL
                     KEKEIFPDGRVRLLLRLAAYAGADWDREIIFCPGRAVFFRDKITARAGGDYVAEVHFR
                     TPAKAVLEGNRLLSRRESAGAGPVDLRIISRSSPDSLRLAARPVELILGVVDRVKDPA
                     GRSVNEKVLSWCRRYRTPEPALTQLTGRRAGYLGPGESFCFEHLVQIRGAGEPELELV
                     EKSGRLLMAAPGWEADLGESEPARPVSVSAAPAVPARLPAARPFAEVEGRITCLRAAA
                     DDGPVCGTDTGGLTRFDAAGRRQWERRLAGPIRDIAILDGPAGAGLIGVGHAADRLSL
                     LDGRGEPLWERRIERVPTHWPWFELETPLVARLIGADFGAGPLLVAGCGDSRLRAFDT
                     SGNERWQFLFLAGVPGRLAAADFDGDGRVEIAAGGEITNMASTCRLLDRDGLLKAELL
                     AEGWTSLLKSLAWGEVDGRPLICLGANHGRNLRVYGLEAMKSGGRPRLIWEELLGGAV
                     LQLRLDPQRPLLWIGTAAGFLIGAEPLTGRFCFTRLLDGAVTGLATRPGGVLVQTAAG
                     SAWLVNAAGEVTARLEPEAARENFLPDGDGGLFFTVGNRLFRLPAETGPSAGGGGKHE
                     "
     gene            6638..7600
                     /locus_tag="PKN80_03065"
     CDS             6638..7600
                     /locus_tag="PKN80_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35026.1"
                     /translation="MSDRVRLALIGAGEIANAMHYPSLAEFPDVELAGLCDLEPERLR
                     ATADRFGIQRTFTDYRRMLDEVEAAAVYLILPPFLVLEPVLECLDRGFHVFIEKPPGV
                     SAAETGRMADRARLRDRLSMVGFQRRFCPLLVESRRRVEERGPVIQCGARYLKNRLGA
                     RPYYDGRIDMLTCDVIHAVDILRWMGGEVRRVVSDIGKCCADYENAFNALLKFESGAV
                     GFLMNNWAVGKRTFSVELHARGISAYAEPDDRAVFYQDNRNQGTELASREAAGSDRPH
                     HYLGFRAENRHFIDCIKEKRQPLTNFEEAVRSMELVELVRRSAW"
     gene            7634..8632
                     /locus_tag="PKN80_03070"
     CDS             7634..8632
                     /locus_tag="PKN80_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35027.1"
                     /translation="MGEKKIRAAVIGCGGFGRNLYLPILKSLAAYETVAAADINLEAA
                     RKCAEEFKLRYATDDPERVFADPEVDAVFIATRHDSHVPLSIRAARAGKHILCEKPMG
                     VNWEECLQLADEVRRSGVRYSIGYNRGLAPLVVKARELLADQAARRLIYHRIQAPFPE
                     RHWIHDPVAGGGRLVGEGCHIFDLLCELVPAPPVTVYACGGTFLDPKLVATPDSAIVT
                     IGFADGSVGTTIVASAGCGRFPKEATEIYCAGRAIHINDFRELGYYGFLENGDGRIQL
                     DAVDKGHRREVALFAAALLEGGPQPNGLEQALRAARISFLAGESIRTGQVLPVTGL"
     gene            complement(8619..>10090)
                     /locus_tag="PKN80_03075"
     CDS             complement(8619..>10090)
                     /locus_tag="PKN80_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35028.1"
                     /translation="SGGVQFGQPYLYLPGRDRPVPVNDTHPYHAIPVSDDSWKYFTTV
                     GKPGKTFVVNKRWNFSLTGARNRLTFVSELGYGDYGFLPEVLEGYRRRRAWKGLNDYR
                     IFAGIWENLAGEYRRLGLEESFGPISRFLSATHAVHAEGNSRMIEGILINPWTAGYTL
                     TFFEEASWECTGGLVTLWNQPKDLMFAAGKRLNADRVLVLEWGPRNFYAGREREVTAW
                     LVDQTRTPHVGRVRLSLTAGGKSAAGELAYRSRGRRIERIGAWQFRAPETAGFGTLLG
                     RLETEGFRWIPAEYSVFVGPAGQLAGLRARTARLPEELRGRLAGSGLAEGAGPILLDG
                     ANAAPKDVQEILAEVENGAAAVIFNPDRVAEKLPFPVNYQPARGWAFPCLHFLEAHPL
                     FAGGRGTGAAGEPFTGVVPYLSADSAGLPAGVRTAGGCLRYSMHGRISSGRDILELRL
                     GKGRLILNSYRLPESIGRDPLAEKLLANLLNYAGAAKGR"
BASE COUNT         1524 a         3368 c         3488 g         1710 t
ORIGIN      
        1 tcggggcgga ccggttcctg ctatactcgg cggtcccggt cgtcctgggg ccgaactcgc
       61 ttcgttcgac caacatctac gacagcgacg gctccctgat cgccctggtg ccggcttacg
      121 cggccatcgg ccggctcatg gccggcgcca ccggcggccg gcggctgccg gttccagacg
      181 agaacctgac tgccttcgcc ttcgaacggg gcggcgagag ccacctgatc ttctgggtgg
      241 acccgggcct gggcgcctac ggaaaccagc aggcgggcca gtaccgcctg gaagtggccc
      301 tggaaggaat ggtgaccgac atctttggca atccctaccc ggttgaaaag ctcggcggcg
      361 gccggaccgg gatcgagttc cagggtcagc cgctgctttt gaatggcggc cgggccggcc
      421 tcgaggcgat tgccaggagc ctggaatcgg ccgtctttca gaactacctg ccggtgcgcg
      481 gttactgccg gctggacgag tcgggccggc cggtcctgcg gctggtgatg gaaaaccgga
      541 ccaggcaacc ctttgctggg gaattgaagg ttgaggagga gcgtggcctg ctggcggagg
      601 ggacctcgtt cacccggacg ctggacctgg ctcccggccg ccgggcgacg ttcgacctgg
      661 cctgccagcc gtcgctgctg ggccggcggc cgctggccaa cggggtggcc gaggtttcca
      721 tgggcagccg tttcaacgcc ggtttcggca tctgggctca gccggccttc cggctggctg
      781 aggtgccgac gccggatggc gatccggccg gctggccggg tgcgccggct tacatctact
      841 ccaccgtccg ggcctccggc ggttacgaac ggagccaggt acgcgagggg gccgagttca
      901 tcacccggga gaagacgctc cagacggcca tcaaagccgg ctgggacgag aataacctct
      961 acctggccat ccggaacgtc gacgacgagt tgaattttgc gcgccaggcg tcggaggcgg
     1021 gacggggcga ccgggtggtg atcggtctag atacccgcct ggaggccgat atctttgata
     1081 tcaggcggaa cgaggacgat ttcctgatcc agctggacgc ggtgggcgag cagggacggg
     1141 cccggctgat tgactggcag ggaaaagcgg tggaacttcc ggtcaatctg aaacgggtgg
     1201 tggcgggggc cgacagcggc tacctcttcc tggtcagctg cccctgggag cggttggggg
     1261 tgaagccgac cgccggcgcg gtgctgggct ttgacgttac gctcttcgag acggacaaag
     1321 accgggcgcc gcggatcgag gcggcttggg ccggcttcgg attcaacctg gggaatccga
     1381 ccggttacgg acagctgatt ctcaagtaaa gacagacgac cggggcccgc tccggccagg
     1441 gaggcctgaa tgtttggacg attgaggcgc ccggcaggat ttctcctgct ggcggttctg
     1501 ctttggtgcc ggccgttggc cgccgaggag ccgctcttcc gtttctgcca gatcagttcg
     1561 cccactatcg ccgccgcccc ggccgacctg gccggcgacc ggagccacta cggacgcaac
     1621 gtcgaaatgc tgaagcagcg ggtcggggtg gtgaacggat tgaagccggc cttcctgctg
     1681 gtggacggaa ccgtcaccgc ggtgggcagc gccgccgatt accggctcct ggccggcatc
     1741 ctgggcgagg tcaaggcgcc ggtctatctc ggcaagggcg acgatcgggg ccgggccggc
     1801 cggattcccg acccggcccg ggtggcgccg ccggccgact tcgaaaagga gaagatcttc
     1861 gattaccggc cgttcttttc gaccgacctc aagggaatcc acctggcctt catcatggag
     1921 ggttacggga cgccggcggc ggccgaattt ctcgagtcgg acctggccgc ggccgagaaa
     1981 cggggcgccc ggggcctgat cgtccttctc aataccaccg aggtttactc ctttgtcgcc
     2041 tggtggcagt cgcggggcaa ggtgccggtc tttccggacg agaagctggc cgccctcttc
     2101 aagaagtacc aggtcagcct ggtggttctg ccgggcgtcc cgggccagac ccagtttcca
     2161 ttcctccggg ccggggatac ctattatgtc agcggccagg ggctggcgat cgatacccgc
     2221 ttttaccctt cccaggggct gatctacgag gtttacccgg accggaccct ggtccggacc
     2281 ataccgcttg acggccagcc gtccctggaa ctgactgcct ggaacggccg cgcacccggg
     2341 ggacgcgccg cccgggaccg ttacctggac aacccttacg gctgcgagag ctatgccgag
     2401 gacctggccg ccaaacccct gctgaccttc gtccagatct ccgacaccca gcacagtccg
     2461 aagcgctggg actcctaccg gcgccgggtg gacgtgctgg cctacgaggc ggcggtggag
     2521 gatatcaacc gccttcggcc ggcctttgtc atcaactccg gcgacctggt cgattacagc
     2581 actcccgaag cctacgccga gtatcagcgg gtaagcaacc tgctggccgt gcccctttac
     2641 gagacgccgg gtaaccacga ccgttacctg ccggaagggc aggcctggcc ggaggggacg
     2701 accctggagg cgaaactggc ccagtacctg aagcataccc ggccgaacgg cctggattac
     2761 gccttcgaga agaacggcgt ccgtttcata tccgtcaaca gcaccgagga ccgggtgaac
     2821 gacctggagt ttctcaaaaa ggcgctcgaa gagtccggca agtcgcgtta tctcttcgtc
     2881 tatacgcact tcccgttcag cgaggtcttc ggaaacgggg tcgccaacgc cgacgaggcg
     2941 gctcctttgc tggccaaata ccagcccacc gccctgctcg gcggccaccg ccaccgtttc
     3001 gcctggcggg tgatcgacgg gattccgaac ttcctggccg acgggatcgc ctgggggagc
     3061 ggcatggccc gggccgacct ggtcggcggc cgggtcatct ccgtctacca tctctacccg
     3121 gaccggctgg tggtgggcat gaagccgctt tacagctcga tctacgcccg gatcacggtg
     3181 ccggtcaagg cgcgccgctg agtcatggct tccggattgg agtgccggtt ggaagcgcgg
     3241 cgggtggagc ggacgacccc cctctccgcg gcaccgggat tcttcatcgc cgctggtttg
     3301 aatggccggg cggcggcccg gcggctggcc ggatgtctcg agcggcacca gcgaacgatt
     3361 cgaatcctgg aagacccgcc gctggcggca ctggaggagg ccggatcggg cctgctgatc
     3421 ggcaacctgg ccgacagtcg ggcggtccgg gatctctatt accgctttct cctggtgacc
     3481 gaccgtggct atcccggtcc cggcggttac gaaatccgca ccctcctcga tccgttcggc
     3541 cgcggtcata acttcgttca cctcggttat tcggaccggg ccgggctgga ggccggcttg
     3601 gcgctgctga ccgaacggct ggccgatccg cttccttact tcagcgaggt aaagttcagc
     3661 cggctgcccg ttccggacgc ggcggcggaa cggcttcgcc gcacacccct gccggccgat
     3721 ccccggttca tcgtccacga cgagttcgga gacctcaagg gttacctggg ctacctgagc
     3781 ggcgagccgg gcttgctggc cgatttccac cgggtcctgg cggccacgat cgaggccggc
     3841 cggatcggcc atctctacgc ctattcccgc ctggtggtct ggcgcctgct ggagtaccag
     3901 ggcttgatcc cggaagaatt gcgccagccc ttcctggaga ccgtctatca atgggccgag
     3961 gggcacgagg gtatcggtcg gctgctggag aaccccggct atacctctcc ccacctgccg
     4021 cgtcagaacc acggactgat gccggccctg gccctgctct acgccgccga ctacttccag
     4081 gtccaccatc cggggctggc cgggccggcc tggtggcggc aggcggccga gacgctcttc
     4141 caaccctatg cctccagctg gaagcaccac gaggattgcg ttatccacgg cttccgactc
     4201 tcccagccga tgctgctggg ccgcggcctg ctggacccgg cgcaccggta cttcgaggcg
     4261 ggcggcgccg cccgggcggc cgaatgcgcc atggtcctgg tcaacaaccg gggcctgacc
     4321 cccaacttcg gcgattcgga cctgatccgt tccttcccgg ggccgagcct gcgggccgcg
     4381 gccgcctact accgggacgg acgctatgcc tatgtcaacg acctggccgc cgatggacag
     4441 cgccgggacg gcctggagct gccggtcttc caggccttcg aggccggcgt ggagtcgaag
     4501 gagccggagc ggccggccgg cggcctgacg gtcatcccgc tcgacccgct catctaccgg
     4561 gtctgggagc gggagccgga actggcggtc ggtttcacgg ccgcgccgcc ggccgcgccc
     4621 attgatgagt gtttcgacaa gctctgcatc cggaccggct ggcgcgagaa tgacgattat
     4681 ttactggtag acgggttggg cggcgccctc cacatctttg accgcaagat cgactgtacc
     4741 cacgcttacg acgacgcggc cgcggtcctg gagtactccc gtttcggcct ggccggcctg
     4801 gtctccgagg attcgctggt cctgaccgac ccggaggacc acacggcggt taccgttacc
     4861 cgggacggcc gccgcggccc ggtgccggcc tacgccgccc tcaaggagaa ggagatcttt
     4921 ccggacggac gggtgcgtct gcttctgcgc ctggccgctt acgccggcgc cgactgggat
     4981 cgggagatca tcttctgccc gggccgggcc gtcttcttcc gcgataaaat taccgcccgg
     5041 gccggcggcg attacgtggc cgaggtccat ttccgcaccc cggcgaaggc cgtcctggaa
     5101 gggaaccggc tgctgagccg ccgggagagc gccggcgccg gcccggtcga cttacggatt
     5161 atttcccggt cgtccccgga ctccctgcgg ctggcggccc ggccggtgga actgatcctg
     5221 ggcgtggtcg atcgggtcaa ggatcccgcc ggccggagcg tcaacgaaaa ggtgctgagc
     5281 tggtgccgtc gctaccggac gccggagccg gccctgaccc agctcaccgg gcgccgggcc
     5341 gggtacctgg gccccggtga aagcttctgt ttcgagcacc tggtccagat ccggggggcg
     5401 ggggaaccgg aactggaact ggttgaaaag tcgggtcgcc tcttgatggc cgcccccggc
     5461 tgggaggccg acctgggaga gtccgaaccg gcccggccgg tatccgtttc ggcggcgccg
     5521 gccgttccgg cccggcttcc ggccgcccgg cccttcgccg aagtcgaagg gcggattacc
     5581 tgtttgagag ccgcggcgga cgacggtccg gtctgcggga ccgatacggg tgggctgaca
     5641 aggttcgatg cggccggccg ccggcagtgg gagcggcgcc tggccggacc gatccgcgat
     5701 atcgcgattc tcgacggtcc ggccggtgcc ggcttgatcg gcgtcggcca cgccgccgat
     5761 cgcctctccc ttcttgacgg ccggggcgaa ccgctctggg agaggcggat cgagcgggta
     5821 cctacccact ggccctggtt tgagctggaa acacccctgg tcgcccggct gatcggcgcc
     5881 gatttcgggg ccggcccgct cctggtggcc ggctgcggtg actcccggct ccgggccttc
     5941 gatacttccg ggaatgagcg ctggcaattc cttttcctgg ccggggtgcc cggccggctg
     6001 gccgcggccg atttcgacgg cgacggccgg gtggagatcg ccgccggcgg cgagattacc
     6061 aatatggcct ccacctgccg gctgctggac cgcgacggcc ttctgaaggc ggagctgctg
     6121 gcggagggct ggaccagttt gctcaagtcg ctggcctggg gagaggttga cggccgtcct
     6181 cttatctgcc tgggcgccaa ccacggccgc aacctccggg tctacggcct ggaggcaatg
     6241 aaatccggcg gccgtccgcg cctgatctgg gaggaactgc tgggcggggc cgtgcttcaa
     6301 ctccggctcg atccgcagcg cccgctgctc tggatcggca ccgccgccgg tttcctgatc
     6361 ggggccgagc cgcttaccgg ccggttttgt tttacccggc tcctggacgg cgccgttacc
     6421 ggcctggcca cccggccggg tggagtcctg gttcagacgg ccgccggttc cgcctggctg
     6481 gtcaatgcgg ccggagaagt aacggcgcgc cttgaacccg aagcggcccg ggagaacttt
     6541 cttccggacg gggacggcgg gctctttttc acggtcggga atcgtctctt ccgattgccg
     6601 gccgaaaccg gcccttcggc cggaggcggg gggaagcatg agtgaccggg tacgcctggc
     6661 gctgatcggc gccggggaga tcgccaacgc catgcattat ccatccctgg ccgagtttcc
     6721 ggacgtggaa ctggccggcc tctgcgacct cgaaccggag cgtctgcggg ccacggcgga
     6781 tcgtttcggt attcaacgga ccttcaccga ttaccggcgg atgctggacg aggtcgaggc
     6841 ggcggcggtc tatcttatcc tgccgccctt cctggtcctg gagccggtcc tggaatgcct
     6901 cgaccgcggg ttccacgttt tcatcgagaa gccgcccggc gtgtcggcgg ccgaaaccgg
     6961 ccggatggcc gatcgggccc gcctccggga ccggctgagc atggtcggtt tccagcgccg
     7021 tttctgcccg ctcctggtcg agtcccggcg tcgggtcgag gaacggggtc cggtcatcca
     7081 gtgcggcgcc cgctacctga agaaccggct gggcgcccgg ccgtattacg acggccggat
     7141 cgacatgctc acctgcgacg tcatccacgc ggtcgacatc ctgcgctgga tgggcgggga
     7201 ggtgcgccgg gtggtgagcg acatcgggaa atgctgcgcc gactacgaaa atgccttcaa
     7261 tgccctgctg aagttcgaaa gcggcgcggt cggcttcctg atgaacaact gggcggtcgg
     7321 caagcggacc ttcagcgtcg agctgcatgc ccgcgggatt tcggcctacg ccgagccgga
     7381 cgaccgggcg gtcttctacc aggataaccg gaaccagggg acggaactgg ccagccggga
     7441 ggcggccggc agcgaccgac cccatcatta cctgggcttc cgggccgaga accgccattt
     7501 catagattgc atcaaggaga agcgtcagcc gttgaccaac ttcgaggagg cggtccggag
     7561 catggaactg gtggaactcg tccgccggtc cgcctggtga ccgcgccggt ttctttcgaa
     7621 aggagcgcaa gagatgggag agaagaagat ccgggcggcc gtgatcggct gcggcggttt
     7681 cggccggaac ctttacctgc cgattttgaa gtccctggcc gcctacgaga cggtggccgc
     7741 ggccgacatc aatctggaag cggcccggaa gtgcgccgag gagttcaagc ttcgttacgc
     7801 caccgacgat ccggaacggg tcttcgccga tccggaggtg gacgcggtct tcatcgccac
     7861 ccgccacgat tcccacgttc ccctgagcat ccgggccgcc cgggccggca agcacatcct
     7921 ctgcgaaaaa ccgatgggcg tcaactggga ggagtgtctt cagctggccg acgaggtgcg
     7981 ccgttccggc gtcaggtaca gcatcggcta caaccgcggc ctggcgccct tggtggtcaa
     8041 ggcccgggaa ctgctggctg atcaggcggc ccggcgcctt atctaccatc gaatccaggc
     8101 gcccttcccg gagcgccact ggatccacga cccggtcgcc ggcggcggcc gcctggtggg
     8161 cgagggctgc cacatctttg acctgctctg cgagctggtc ccggcgccgc cggtgaccgt
     8221 ctacgcctgc ggcggcacct tcctggaccc gaaactggtg gccacgccgg acagcgccat
     8281 cgttaccatc ggctttgccg acggttcggt cgggaccacc attgtcgcca gcgccggctg
     8341 cggccgtttt cccaaggagg ccaccgagat ctactgcgcc ggccgggcga tccacatcaa
     8401 cgatttccgg gaactgggct actacgggtt cctggagaac ggggacggcc ggatccagct
     8461 ggacgcggtc gacaaggggc accgccgcga agtggcgctc ttcgcggccg ccctcctgga
     8521 aggcggtccc cagcccaacg gactggagca ggccctgcgg gcggcccgga tcagcttcct
     8581 ggcgggagag tcgatccgga ccgggcaggt gctgccggtt accggccttt agccgcgccg
     8641 gcgtagttga gcaggttggc cagcagtttt tcggccagcg ggtcccggcc gatcgactcc
     8701 ggcaggcggt aggagttcaa aatcaggcgt cccttgccca gccgcagctc caggatgtct
     8761 cggccgctgc tgatgcggcc gtgcatgctg taacggaggc agccgccggc ggtccggacc
     8821 ccggccggca atccggccga gtcggcgctc aggtaaggga ccaccccggt aaagggttcc
     8881 ccggccgccc cggttccccg gccgccggcg aagagcggat gcgcctccag gaagtgcagg
     8941 cagggaaagg cccagccgcg ggccggctgg tagttgactg gaaagggcag tttctccgcg
     9001 acccggtccg ggttgaagat caccgccgcc gccccgttct ccacctccgc caggatttcc
     9061 tggacatctt tcggagccgc gttcgccccg tccagcagga tcggacccgc cccctccgcc
     9121 agcccgcttc cggccaggcg gccgcgcagt tcttccggca gccgcgcggt ccgggctctt
     9181 aagccggcca gctggccggc cggtccgacg aagaccgagt attcggccgg gatccagcgg
     9241 aagccttcgg tctccaggcg gccgagcagg gtgccgaagc cggccgtttc cggggcgcgg
     9301 aattgccagg cgccgatcct ttcgatccgg cggccgcgcg agcggtaggc cagttccccg
     9361 gcggccgatt tgccgccggc ggtcaggctg agccggaccc gtccgacgtg gggcgtccgg
     9421 gtctggtcga ccagccaggc ggtcacctcg cgctcccggc cggcgtagaa gttgcgcggt
     9481 ccccattcca ggaccagcac ccggtcggcg ttcaaccgct tcccggccgc gaacataaga
     9541 tccttgggct ggttccagag ggtgaccagt ccgccggtgc actcccagga ggcctcctcg
     9601 aagaaggtca gcgtgtaccc ggccgtccag gggtttatca agattccctc gatcatccgg
     9661 ctgtttccct cggcgtggac ggcgtgggtg gcggagagaa agcggctgat cggaccgaag
     9721 gactcctcca ggccaagccg gcggtattcc cccgccaggt tctcccagat gccggcgaag
     9781 atccggtaat cgttcaaccc cttccaggcg cgccggcggc ggtacccttc caggacctcc
     9841 ggcaggaatc cgtagtcgcc gtaccccagc tccgagacga aagtcagccg gttccgggcc
     9901 ccggtcagcg agaagttcca gcgtttgttg accacaaagg tcttgcccgg ttttccgacg
     9961 gtggtgaagt atttccagga gtcgtcgctg accgggatcg catggtaggg gtgggtgtcg
    10021 ttgaccggaa ccgggcggtc ccggccgggc aggtagaggt agggctggcc gaactggacg
    10081 ccgccggagt
//
LOCUS       DAOQYN010000047        10039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_7189_length_10039_cov_5.309395, whole genome shotgun sequence.
ACCESSION   DAOQYN010000047 DAOQYN010000000
VERSION     DAOQYN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7189_length_10039_cov_5.309395"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PKN80_03080"
     CDS             <1..808
                     /locus_tag="PKN80_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNQ35029.1"
                     /translation="IESFIQTDASINPGNSGGPLVNLKGEVIGINTAMAGGQGLGFAV
                     PCNIARNVLDQIRSKGKVVRGWLGISMQPVTPDMIEGLKLKEARGVLVSNVFAGEPAD
                     RAGLKAGDIIVRVGETTVAGTDELQMLVGSRMPGEKLSLVVLRDGKEVPLTVQLGEQP
                     ADLSARGGAPQEVPDWRGIKVGAVTEEIARQLSLPKAEGVLVNEVASDSPASGKLAPG
                     VVILEINREPVRSLADYQRLAARVAADKAALLRVYNQGQYFYLVVPPPGK"
     gene            complement(880..2082)
                     /locus_tag="PKN80_03085"
     CDS             complement(880..2082)
                     /locus_tag="PKN80_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35030.1"
                     /translation="MAEKKVIRTAVIGAGGRGKYFGRLYRKEAHPGFDLRAICDLDPD
                     RLKEAREFFGDGIAYRTDSAKIYADPEIDAVLVATDDRNHVEPTLAGLAAGKHVLVEK
                     PLCQTTADAGRIIAAARKSPGIFMIGFELRECTVFQSMKRLLKEGRIGPVLVAHSFDN
                     VSVGGHYFFHSPHRQKAYYRSLLLQKACHSLDLLNWFVDSEPVKVYGIGGLDFYGRTA
                     DPKLRCRDCDRADCPYRISLESFQMDYDARVRITDFCVWSKELNLNDNSELCISYASG
                     AKATFHECHFTPEYSREFWLVGPKGKMYGYYDNPGRFPIRIEYSHDRERRTEEFKPPH
                     TGGSHGGGDLRLRDEFYRRIVENDPPTEVQDSAYYSTALAICAEESIESGRPVEIPGL
                     KSVLAASD"
     gene            complement(2259..3932)
                     /gene="hcp"
                     /locus_tag="PKN80_03090"
                     /gene_synonym="priS"
     CDS             complement(2259..3932)
                     /gene="hcp"
                     /locus_tag="PKN80_03090"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNQ35031.1"
                     /translation="MEGSMFCRQCEQTVGGKGCTQRGVCGKSPEAADLMDALIHLMKG
                     IAWWGRQARELEVKDTEVDHFLMEGLFLTVTNVNFDVADLEAWVRRAVKMRGKANELF
                     RSAYRSRQGRDFAGPVPAAVEWTPAAALGDLVVQSREVGILADPGLDEDIRSLREILL
                     YGLKGMAAYAHHAARLGKESEEVNAFLHAGLAALLDADRSAGDLIKLIMDFGLVNLKC
                     MEMLDGAHTSRFGHPEPTEVWLGTRQGPAILVSGHDLRDLEELLEATKDTGISIYTHG
                     EMLPAHGYPKLKKYPHLAGHYGGAWQEQQKEFDLFPGAILMTTNCIQKPRPTYQDRIF
                     TTGLVGWPGLNHIPETGGPKDFGPVIKKALEAGGFPAEAAGKKILVGFGHQAILSVAG
                     TVVEAVKTGQIRRFLLIGGCDGAKPGRNYYTELAQLAPQDCLILTLACGKFRFNKLEF
                     GTVAGLPRLLDCGQCNDAYSAIKVAAALAEAFKCGLNDLPLSLVLSWYEQKAVCILLT
                     LLALGIRDIRLGPSLPAFVSPNVLKVLVEKFNIQPITTPEADLKAILGN"
     gene            complement(3901..4395)
                     /locus_tag="PKN80_03095"
     CDS             complement(3901..4395)
                     /locus_tag="PKN80_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNQ35032.1"
                     /translation="MKLLTKNSDYAVRALLILARAGDGYLSSRELAGREKIPLAYIRQ
                     VLHGLVDLGLVEAREGARGGVRLRRDPDQIPLELLIRHFQGGLTFSECLFRKKFCRNR
                     SGCVLRRRLLELEEEVVERLKGVTIGTLLDDLDRKEGKPGRPVTGKTGKGGQDGRKHV
                     LPAV"
     gene            complement(4465..5442)
                     /locus_tag="PKN80_03100"
     CDS             complement(4465..5442)
                     /locus_tag="PKN80_03100"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNQ35033.1"
                     /translation="MFIKILLRVFSIFLMIMLGAIARRRGILDSEATRRLALSVTNFF
                     YPAMIFSSLVRNFDLASLLRNWTLPAGTIMIMLLGYLVGLAALRVLDFEKPAEGHAFH
                     FQCTINNYSFLPLPIILLFWGNAGVAGLLFSSLGSEISVWTFGVLALTGNRLRRDSLR
                     NLLSLPMLSIAAAILTIVVRDFLAANGALPAAGSLPAEAAAALMNVLDLFGKATVPLA
                     MFQVGSRISELTPRHLFTLNQFYVVLLRLTLIPALALILLFSLPLDPLPRLVLGMVAI
                     MPGAIASVVLSDVYGADREFAASSVMATHLFSLLTIPAWMTLLLASAGR"
     gene            complement(5458..6546)
                     /locus_tag="PKN80_03105"
     CDS             complement(5458..6546)
                     /locus_tag="PKN80_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ35034.1"
                     /translation="MSRILVSPLSWGLGHATRDIPIIRELLEHGHRVSIAGSGRAMEL
                     LKQEFPHCDFFELEDYPPPYTATRYFLAKFIAYIPLILHAIRLEHESTRRLLAEHPCD
                     LIISDNRFGVHAPGVPSFFISHQLRFYVPDYLRPGGYISARFNSSCHRNFRRVIVPDN
                     PPEKGSLSGLLSENRFPATQERLYYAGILSSVDPVAAAEDIDYLISISGPEPQRSKLE
                     ELVLQQAAKLPGRKVILLGLPAETRRFQLDERTEVRNHATREEMAALMSRAKFIITRS
                     GYTTMMELAELGKRRALLIPTPGQTEQEYLSLYYQQQGWFYSQSQYRLDLVRDIQATS
                     DYRGFPAVTPSRENARRLYRELFAPHLD"
     gene            complement(6563..7576)
                     /locus_tag="PKN80_03110"
     CDS             complement(6563..7576)
                     /locus_tag="PKN80_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNQ35035.1"
                     /translation="MTKETRSLGQRLISLEEGQTFLRRGWHASDLHVHTFFSHDVLPS
                     RSLDPLTLYEKARRQGFRYSTFTDHDTMAAYDRIGWTREGLVPGVEITIRDRKRVGHT
                     LHINVYQLNRDQFQDLDAIRREANLEKLVAYLKSADLPFIYNHPFWFETRERPSYAVI
                     QDVIHLFPVVEYNMHRVKQKNRLTLKLARKYGKGLVAATDTHIGDLGRAQTFAPGETF
                     REFYDNIRAGNACLLAQDLTLDSLKKEMNAWIELIFKLDITTIEKTKYTGIKPIDCFI
                     NTFTRSRLKKSTPINRTARVVARSISKTGAPAIFYLKKQNLTARHIDRQLDLDPDLDL
                     ELG"
     gene            complement(7662..8984)
                     /locus_tag="PKN80_03115"
     CDS             complement(7662..8984)
                     /locus_tag="PKN80_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNQ35036.1"
                     /translation="MGRKVSLGIALLLVALAFGWLATEIGAAEPGGKFDYGLGLRVRQ
                     EYLENATDLNLAGLEEENYFRLKTSLWGKWQFTEKASLYTKLTNESRYYINSNTDRFD
                     NEWLGDEVFFDNLYLDLKNLAGGALDLRLGRQDFLGTFGEGFLVMDGTPGDGSRSFYF
                     NAAKATWRINERNSLDVVFLNNPDQDEFLPVYNDKEKLLNFSDEAALILYGRLQNSEP
                     LRFEPYYIYKTEDSRGALPDLEINTLGLRTVYNQGPWNLGGELAYQFGDYDGPTDREG
                     TGLNLFLNRKFEAARLQPELQFKFVYLSGDDAGTVDNEGFDPLFSRFPWLSELYLYNY
                     APEQGWPGYWTNLTFYRAGVKLNLNPKLSLTFYENYLRANEKTNLASAVFSNDGRERG
                     WLSQLWLNFVINPKVTGHLQLEYLKPGDFYSAGADPCAFLRWEFNIQF"
     gene            complement(8995..9684)
                     /locus_tag="PKN80_03120"
     CDS             complement(8995..9684)
                     /locus_tag="PKN80_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35037.1"
                     /translation="MKLKLNPLGPETPPRAEGGFKVLLLAGAVLALALQPAPAAADGS
                     ILGGGQVEIQGKALPLGYAQVVLLNETPDLEAVEILARRAAAKDRQPERAYHQHRAFY
                     LIRAIEDGRRLGVSQVRTAVLSKKTDGRGAFNLRYLKPGTYYLGVYRTFRNRDIAVWL
                     LPVQVAEGRVTQVSLDNTNAFEVYWPNRYPATAAPAAPALPKPEPAPETKTETEAKPP
                     AAPEAAPTATP"
BASE COUNT         1678 a         2989 c         3491 g         1881 t
ORIGIN      
        1 gatcgagagc ttcatccaga ccgatgcctc gatcaacccc ggcaacagcg gcgggccgct
       61 ggtcaacctc aagggcgagg tgatcggcat caacaccgcc atggccggcg gccagggtct
      121 gggtttcgcg gtgccctgca acatcgcccg caacgtcctg gaccagatcc gcagcaaagg
      181 caaggtggtc cgcggctggc tgggcatctc gatgcagccg gtcacgccgg acatgatcga
      241 gggattgaag ctcaaggagg cgcgcggggt cctggtgagc aacgtctttg ccggcgaacc
      301 ggccgaccgg gccgggctca aggccggcga catcatcgtc agggtcggtg agacgacggt
      361 ggccggcacc gacgagctcc aaatgctggt cggctcccgg atgccgggcg aaaagctgtc
      421 gctggtggtc ttgcgggacg gcaaggaggt tcccctgacc gtccagctcg gcgagcagcc
      481 ggccgatctt tccgcccggg gcggcgctcc ccaggaggtt ccggattggc ggggcatcaa
      541 ggtcggcgcg gtcaccgagg agatcgcccg gcagctgtcg ctgcccaagg ccgaaggggt
      601 gctggtgaac gaggtcgcct ccgacagccc ggcctccggg aagctcgccc ccggggtggt
      661 catcctggag atcaaccgcg aaccggtccg cagcctggcc gattaccagc ggctggcggc
      721 ccgggtggcg gccgacaagg cggccctgct gcgggtctat aaccaggggc agtacttcta
      781 cctggtggtg ccgccgccgg gcaagtagcg gaaggttctt ttgccaggcc cggccgggat
      841 ggaataatcc ggccgggcct ttttttttat gccttttaat cagtccgagg cggccaggac
      901 cgatttcagg ccggggatct cgaccggccg gccgctctcg atcgactctt cggcgcagat
      961 ggccagggcg gtcgagtagt aggccgagtc ctggacctcg gtgggcgggt cgttttcgac
     1021 gatccggcgg tagaactcgt cgcgcaggcg caggtcgccg ccgccgtggg agccgccggt
     1081 gtggggcggc ttgaactcct cggtccgccg ctcccggtcg tgcgaatact cgatccggat
     1141 cgggaagcgg cccgggttgt cgtagtaacc gtacatcttg cccttgggcc cgaccagcca
     1201 gaactcccgc gagtactccg gggtgaagtg gcactcgtgg aaggtggcct tggcgccgct
     1261 ggcgtaactg atgcagagtt ccgaattgtc gttcaggttg agttccttgc tccagacgca
     1321 gaaatcggtg atcctgaccc gggcgtcgta gtccatctgg aacgactcca ggctgatccg
     1381 gtagggacag tcggcccggt cgcagtcgcg gcagcgcagt ttcgggtcgg ccgtccggcc
     1441 gtagaagtcc agcccgccga tgccgtacac cttgaccggc tcgctgtcca cgaaccagtt
     1501 gagcaggtcc agcgagtggc aggccttctg gagcaggagc gaccggtagt aggccttctg
     1561 gcggtgcgga ctgtggaaga agtagtggcc gccgaccgaa acgttgtcaa acgagtgggc
     1621 caccagcacc gggccgatgc gcccctcctt gagcagccgc ttcatgctct ggaagacggt
     1681 gcactcccgc agttcgaagc cgatcatgaa gattccgggg cttttgcggg cggcggcgat
     1741 gatccggccg gcgtcggcgg tcgtctggca gagaggcttc tccaccagca cgtgcttgcc
     1801 ggcggccagt ccggccaagg tcggctcgac gtggttgcgg tcgtcggtgg ccaccaggac
     1861 cgcgtcgatt tccgggtctg cgtagatttt ggccgagtcg gtccggtagg cgatcccgtc
     1921 gccgaagaac tcccgggcct ccttcagccg gtcggggtcc aggtcgcaga tggcccgcag
     1981 gtcgaagccc ggatgggcct ccttccggta gaggcgtccg aagtacttgc cccggccgcc
     2041 ggcgccgatc accgcggtgc ggataacctt tttttcagcc attcgaggtc cctcgaggtt
     2101 tgcgtgggca ggagacttat aaaatatatt ttaattattt taaaggccgg ttcgggtttt
     2161 gtcaacctga tttttcatgc attatccccc ctcaaaaaaa acggcgcggc tccggaaagg
     2221 gaaccgcgcc gtttcttttt acggacgccg ccgccggatc agttgcccag gatggccttc
     2281 aggtccgcct ccggcgtggt gatcggctgg atgttgaact tctccaccag caccttgagc
     2341 acgttcggcg agacgaaggc cggcagcgag gggccgagcc ggatgtcccg gattcccagc
     2401 gccagcaggg tcagcaggat gcagaccgcc ttctgctcgt accaggagag gaccagcgag
     2461 agcggcaggt cgttcaggcc gcacttgaag gcctcggcca gggcggccgc caccttgatg
     2521 gccgagtagg cgtcgttgca ctggccgcag tccagcaggc ggggcagccc ggccaccgtt
     2581 ccgaactcca gcttgttgaa gcggaacttg ccgcaggcca gcgtcaggat caggcagtcc
     2641 tggggtgcca gctgggccag ctcggtgtag tagttgcggc cgggcttggc cccgtcgcag
     2701 ccgccgatga gcaggaagcg tcgtatctgg ccggtcttga cggcttcgac caccgttccg
     2761 gccaccgaga ggatcgcctg gtggccgaaa ccgaccagga ttttcttgcc ggccgcctcg
     2821 gccggaaaac cgccggcttc cagggccttt ttgatgaccg gaccgaaatc cttcgggccg
     2881 ccggtttccg ggatatggtt aaggccgggc cagccgacca gcccggtggt gaagatgcgg
     2941 tcctggtagg tcgggcgcgg tttctggatg cagttggtcg tcatgaggat cgcgcccggg
     3001 aagaggtcga actccttctg ctgttcctgc caggcgcccc cgtagtggcc ggccaggtgc
     3061 gggtacttct ttagtttcgg gtaaccgtgg gccggcagca tctcgccgtg ggtgtagatg
     3121 ctgatgccgg tatctttggt cgcttccagc agttcctcca ggtcgcgcag gtcgtggccg
     3181 gagaccagga tggccggacc ctggcgggtg cccagccaga cctcggtggg ttcgggatgt
     3241 ccgaagcggc tggtgtgagc cccgtcaagc atttccatgc acttcaggtt gaccaggccg
     3301 aaatccatga tcagcttgat caggtcgccg gccgatcggt ccgcatcgag cagggcggcc
     3361 aggccggcgt gcaggaaggc gttgacctcc tccgattcct tgccgagccg ggcggcgtgg
     3421 tgggcgtagg cggccatgcc cttcagaccg taaaggagga tttcccgcag gctgcggatg
     3481 tcctcgtcca ggcccggatc ggccaggatg ccgacctccc ggctctggac gaccaggtcg
     3541 ccgagcgcgg cggccggagt ccactcgacg gccgccggca ccggtccggc gaaatcccgg
     3601 ccctgccggg agcggtaggc cgaccggaag agttcgttgg ccttgccgcg catcttgacc
     3661 gcccggcgca cccaggcttc caggtcggcc acgtcgaagt tgacgttggt gacggtgagg
     3721 aagaggcctt ccatcaggaa atgatcgacc tcggtgtctt ttacctccag ttcccgcgcc
     3781 tggcgtcccc accaggcgat gcccttcatc aggtggatga gcgcgtccat caggtcggcg
     3841 gcctcggggc tcttgccgca gaccccgcgc tgcgtgcagc ccttgccgcc gacggtctgt
     3901 tcacactgcc ggcagaacat gcttccttcc atcttgccct ccttttccgg ttttaccggt
     3961 taccggccgt cccggttttc cttccttgcg gtcaaggtcg tccaggagcg tgccgatggt
     4021 gacgcctttc agtctttcaa ccacttcttc ttccagttcc agcaggcgtc ggcgcagcac
     4081 gcatccggaa cggttccggc agaatttctt gcggaacagg cactcggaga aggtcagtcc
     4141 gccctggaag tggcgtatca gcaattccag ggggatttga tccgggtccc gccggagccg
     4201 taccccgccc cgggctcctt cgcgggcctc aaccagtccg aggtcgacca gcccgtgcag
     4261 cacctggcgt atgtaggcca gcgggatctt ttcccggccg gccagttccc ggctcgagag
     4321 atagccatcg ccggcccggg ccaggatgag cagcgcccgg accgcgtaat cgctgttctt
     4381 ggtgagcaat ttcataactg atacaattat agtattagtt tacccgcgtt gtcaagccgg
     4441 gcggaaaagg ggagcgtcgt ccggtcagcg gccggccgag gccagcagca gggtcatcca
     4501 ggccgggatg gtgagcaggg agaagaggtg ggtggccatc acgctggagg cggcgaattc
     4561 gcggtcggcc ccgtagacgt cgctcaggac gacgctggcg atggcccccg gcatgatggc
     4621 caccatcccc agtaccaggc ggggcagcgg gtccagcggg agcgagaaga gcaggatcag
     4681 ggcgagggcc ggtatcaggg tcaggcgcag caggaccacg taaaactggt tgagggtgaa
     4741 caggtggcgc ggggtgagtt ccgagatccg gctgcccacc tggaacatcg ccagcggcac
     4801 ggtggccttg ccgaagagat ccaggacgtt cattaaggcc gcggccgcct cggccggcag
     4861 gctcccggcc gccgggaggg cgccgttggc ggccaggaag tcgcggacaa cgatggtcag
     4921 gatcgccgcc gcaatggaaa gcatcggcag gctcagcagg ttgcgcaggc tgtcgcggcg
     4981 cagccggttg ccggtgaggg ccagcacgcc gaaggtccag accgaaatct ccgagccgag
     5041 cgacgagaag agcagtccgg ccacgcccgc gtttccccag aagagcagga tgatcggcag
     5101 cggcaggaag gagtagttgt tgatcgtgca ctggaaatgg aaggcgtgcc cctcggccgg
     5161 cttttcgaaa tcgagcacgc gcagggccgc caggccgacc aggtagccca gcagcatgat
     5221 catgatcgtc ccggccggca gcgtccagtt gcgcagcagg gaggcgaggt cgaaattccg
     5281 gaccagcgag gagaagatca tggccgggta gaagaaattg gtcaccgaga gggccagccg
     5341 ccgggtagcc tccgaatcca ggatcccgcg gcggcgggcg atcgcgccca gcatgatcat
     5401 caggaagatc gagaagaccc ggagcaggat ttttatgaac atggcggccg tttgcggtca
     5461 gtccaggtgg ggcgcaaaga gttcccggta gaggcggcgg gcgttctccc gcgagggggt
     5521 gaccgccggg aacccccggt aatcgctggt cgcctggatg tcccggacca ggtccagccg
     5581 gtactggctc tgggagtaga accagccctg ctgctggtaa taaagggaga ggtactcctg
     5641 ctcggtctgg ccgggagtgg gaatgagcag ggcccggcgc ttgcccagtt cggccagttc
     5701 catcatggtc gtgtacccgg agcgggtgat gatgaatttg gcccggctca tcagggccgc
     5761 catctcctcc cgggtggcgt ggttgcggac ttcggtccgc tcgtccagct ggaaacgacg
     5821 cgtttcggcg ggcaggccca gcaggatcac cttgcggccg ggcagcttgg ccgcctgctg
     5881 caggaccagt tcttccagct tggaacgctg gggttccggt ccggagatgc tgatcaggta
     5941 gtcgatgtcc tcggccgccg cgaccgggtc gacgctggag aggatgccgg cgtagtagag
     6001 gcgttcctgg gtggccggga agcggttctc cgagaggagg ccgctcaggg agcctttttc
     6061 cggcgggttg tccggcacga tcacccgcct gaaattacgg tggcaggagg agttgaaccg
     6121 ggccgagatg taaccgcccg gccgcaggta gtcgggcacg tagaatctca gctggtggga
     6181 gatgaagaag gagggcaccc cgggtgcgtg caccccgaag cggttgtcgc tgatgatcag
     6241 gtcgcagggg tgttcggcca gcaggcgccg ggtcgattcg tgttccagcc ggatggcgtg
     6301 gaggatcagc gggatgtagg cgatgaactt ggccagaaag taacgggtgg ccgtgtaggg
     6361 cggcggataa tcctccagtt caaagaaatc gcagtgcggg aattcctgct tcagcagttc
     6421 catcgcccgg ccggaaccgg cgatcgaaac ccggtggccg tgttcgagca gttcccggat
     6481 gatcgggatg tcgcgggtgg cgtggcccag gccccaggag agcgggctga ccagaatgcg
     6541 ggacatggtg gcgcgtcccc ggttaaccca gttcgagatc caggtccggg tccaggtcga
     6601 gctggcggtc gatgtggcgg gcggtcaggt tctgcttctt caggtagaag atggccggcg
     6661 cgccggtttt ggagatggac cgggcgacca cccgggccgt ccggttgatg ggcgtcgatt
     6721 tcttgagccg gctgcgggtg aaggtgttga tgaagcagtc gatcggcttg atgccggtgt
     6781 acttggtctt ttcgatggtg gtgatgtcga gcttgaagat gagctcgatc caggcgttca
     6841 tctccttctt caggctgtcc agggtcaggt cctgggccag caggcaggcg tttccggccc
     6901 ggatgttatc gtagaactcc cggaaggttt cgcccggcgc gaaggtctgg gcccggccca
     6961 ggtcgccgat atgggtgtcg gtggccgcga ccagtccctt gccgtacttt cgggccagtt
     7021 tcagggtcag gcggttcttc tgcttgaccc ggtgcatgtt atactccacg accgggaaga
     7081 gatggatcac gtcctggatg accgcatagc tcggccgctc ccgggtttcg aaccagaagg
     7141 ggtggttgta gataaagggc aggtcggccg acttgagata ggccaccagc ttttccaggt
     7201 tggcctcgcg ccgaatggcg tccaggtcct ggaactggtc ccggttcaac tggtagacgt
     7261 tgatgtgcag ggtgtgcccg acccgcttgc ggtcccggat cgttatctcc acgcccggta
     7321 ccaggccctc ccgggtccag ccgatccgat cgtaggcggc catggtgtcg tggtcggtaa
     7381 aggtgctgta gcgaaatccc tggcgccggg ccttttcgta gagggtcagc ggatccagcg
     7441 agcgggaggg cagcacgtcg tgggagaaga aggtatggac gtgcaaatcg ctggcgtgcc
     7501 agccccggcg caggaatgtc tgtccttctt cgaggctgat caggcgttgt ccgagggatc
     7561 gggtttcctt ggtcatcgtc tcgtggttct tcttaatgtt tggattcagt cccgggttgg
     7621 ccggcggccg ctctctggcg gccgccggcc cggatcccgg tttagaactg gatgttgaat
     7681 tcccagcgca ggaaggcgca gggatcagcg ccggccgagt agaaatcgcc cggtttcagg
     7741 tactccagct ggagatggcc ggtaaccttg ggattgatga cgaaattgag ccagagctgt
     7801 gagagccagc cgcgttcccg gccgtcgttc gagaagaccg cgctggcgag gttggtcttc
     7861 tcgttggccc gcaggtagtt ttcgtagaag gtcaggctca gtttcgggtt gaggttcagc
     7921 ttgaccccgg cccggtagaa ggtcaggttg gtccagtaac cgggccagcc ctgttccggg
     7981 gcgtagttgt aaaggtagag ttcgctcagc cacgggaagc gggagaagag cgggtcgaaa
     8041 ccctcgttgt cgacggtgcc ggcgtcatcg ccggagagat agacgaattt gaactggagt
     8101 tccggctgga gacgggccgc ttcaaacttc cggttgagga aaagattcag tccggtccct
     8161 tcccggtcgg tcggaccgtc gtaatcgccg aactggtagg ccagctcgcc gcccaggttc
     8221 cagggaccct gattgtagac ggtccgcagg ccgagggtat tgatctccag gtccggaagg
     8281 gcgccgcggc tgtcctcggt cttgtaaata tagtaaggtt cgaagcgcag gggctcgctg
     8341 ttctgcagcc ggccgtaaag gatgagcgcc gcctcgtccg agaagttcag cagtttttcc
     8401 ttgtcgttgt aaaccggcag gaattcatcc tggtcggggt tgttcaggaa gaccacgtcc
     8461 aggctgttgc gctcgttgat gcgccaggtg gccttggcgg cgttgaaata gaaggagcgg
     8521 ctgccgtcgc cgggggtgcc gtccatgacc aggaatcctt cgccgaaggt gccaagaaaa
     8581 tcctggcggc ccaggcgcag atccagggcg ccgccggcca ggttcttgag gtcgaggtaa
     8641 aggttatcga agaagacctc gtcacccagc cactcattat cgaaacggtc ggtgttggag
     8701 ttgatgtagt aacgcgactc gttggtgagt ttggtgtaca ggctggcctt ttcggtgaac
     8761 tgccattttc cccagagcga agtcttgagg cggaagtaat tttcctcctc caggccggcc
     8821 aggttcaggt ccgtggcgtt ttccaggtac tcctggcgga cccggaggcc gagtccgtaa
     8881 tcaaacttcc cgcccggttc ggccgccccg atttccgtgg ccagccaacc gaaggcaagc
     8941 gcgacgagca aaagcgcaat tcccaacgag acctttctgc ccatcatccc ccccttacgg
     9001 tgttgccgtc ggcgccgctt caggcgcggc cggcggtttt gcttctgttt ccgtttttgt
     9061 ttccggggcc ggttccggct ttggcagcgc cggcgcggcc ggcgcggcgg tcgccgggta
     9121 gcggttcggc cagtaaactt cgaaggcgtt ggtgttgtcg aggctgacct gggtcacccg
     9181 tccttcggcc acctggaccg gcagcagcca gacggcgatg tcacggttgc ggaaggtccg
     9241 atagacgccc aggtagtagg tgccgggttt caggtagcgg agattgaagg cgccccggcc
     9301 gtcggtcttc ttgctgagga ccgcggttcg tacctggctc acccccaggc gtcggccgtc
     9361 ttcgatcgcc cggatcaggt aaaaggcgcg gtgctggtgg taggcccgtt ccggctggcg
     9421 gtccttggcg gcggcccgcc gggccaggat ttcgaccgcc tccaggtcgg gcgtttcgtt
     9481 cagcaggacc acctgggcgt aaccgagcgg aagagccttg ccctggattt cgacctgtcc
     9541 gccgcccagg atgctgccgt cggccgcggc cggggccggc tggagcgcca gcgcgagaac
     9601 cgctccggcc agcagcaaaa ccttgaagcc cccttccgcc cgggggggcg tttccggacc
     9661 gagcggattt agtttgagtt tcatattcat ctataatata ataaacccgt aaaaggggtc
     9721 aaataggggc gcggcggcct tcgattcggg gcgccgaggg tccggactcg gctcgggcgc
     9781 tgcgccgcgg gggaaaggta cgcttaaatc cggcgcgcgg ctaactcgcc gtccgcttgg
     9841 ggccggaagg cggccgtcgc ggcctggctc agacaggagc cgcgggagga tgatatgccg
     9901 gagcgctgcg cttttaagct tctttcccga ggccgcggct ccgcgactca cctcgttccg
     9961 gaccctcggc ttcaggggaa agacaaaaaa gcaactccgc gacggcgctc accgaaggcc
    10021 gccgcttaaa gggaaagga
//
LOCUS       DAOQYN010000048        10033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_7195_length_10033_cov_4.373822, whole genome shotgun sequence.
ACCESSION   DAOQYN010000048 DAOQYN010000000
VERSION     DAOQYN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7195_length_10033_cov_4.373822"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..778
                     /locus_tag="PKN80_03125"
     CDS             146..778
                     /locus_tag="PKN80_03125"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNQ35038.1"
                     /translation="MRKKGWHIPEETIRRLSLYLRTLRYLEKEGVRVISSERLTKHLH
                     ITSAQARKDLAYFGSFGHPGVGYNVRRLREKIEEILGLDRHWDVILVGAGKMGAAFLA
                     YPGFRRFGFKIRAAFDNNPAKIGQKIGGIPVLDDQRMEEFIQANRIRAAIITVPASAA
                     QDVARRLVGSGITAILNFAPRYLSIPEHIHIKSVDVAMELENLVYYLTNS"
     gene            complement(785..1753)
                     /locus_tag="PKN80_03130"
     CDS             complement(785..1753)
                     /locus_tag="PKN80_03130"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNQ35039.1"
                     /translation="MTKTRRIGLLTTGGDAPGMNPAIRAVVRRALAFGWEVVGIERGF
                     QGLYEKDFREMGHGSVSGIINLGGTVLKTIRCPGFRKADFRRACYRNLEQRGIGSLVV
                     IGGDGSLAASQTLVAEWKHPLAFIPASIDNDISGTTETIGFDTAVNTALEAIDRIRDT
                     ATSHERIFLVEVMGREKGFLALEVGLAAGAEAILVPEVPFDLEAIRELLLADRAKGKR
                     SMIMVVAEGAVEVAQLAPRIAEFYPGEVRYSVLGYIQRGGAPSARSRCLALRFGARAV
                     EALAEGRQAVCVGLEKEKVVVVDLKKARKRLKLDRAAYRLAHVLSA"
     gene            complement(1750..2775)
                     /locus_tag="PKN80_03135"
     CDS             complement(1750..2775)
                     /locus_tag="PKN80_03135"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNQ35040.1"
                     /translation="MKIGILTGGGDCPGLNPAIRGCVYRALDFGYECVGFLDGWKGPY
                     NAVTIPLGLKEVEPLVDRGGTILGTSRFNPFKKEGAPEQVEANLKKLGIDALVAMGGE
                     DTLGVANKLHQRGVKVVGVPKTMDNDLSATDYTFGFDTSVTRALDACRALLDTGTSHR
                     RIMVLEVMGRHAGWVALYTALGAGADWALLPEFKPDYAAMAAHLKQVFARKKVALVVV
                     SEGTEFSEDGEEPLGEKDAFGHIILKKRNLGEKIADYIEKETGVETRSAVIGHMQRGG
                     PPTLFDRILGLRVGVKAAELVSEGKFGMMAALRGQEVIGVPLAEALANLKVVDKAWLD
                     LARVFFK"
     gene            complement(2872..3318)
                     /gene="purE"
                     /locus_tag="PKN80_03140"
     CDS             complement(2872..3318)
                     /gene="purE"
                     /locus_tag="PKN80_03140"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNQ35041.1"
                     /translation="MAEVVIVVGSKSDLPALAPAEETLKASGIDYEVKVLSAHRQPDE
                     TAAFAQQAAGRGVRVIIAAAGLSAALPGFIASHTTLPVLGVPLAVGPLNGLDALLAIL
                     QMPRGIPVATLGLGSQGPQNAALLAARILALAAPGVATRSGRKVRR"
     gene            complement(3326..4483)
                     /locus_tag="PKN80_03145"
     CDS             complement(3326..4483)
                     /locus_tag="PKN80_03145"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNQ35042.1"
                     /translation="MDIRFNERLNRLPPYLFIEIDRRKAELRQAGVDIIDFGIGDPDT
                     PTPDFVVGKMQESVANPAGHRYPLGRGKPVFREAIAAWFRGRFGVELDPAGEVLFLIG
                     SKEGIGHLPFAFLDRDDICLVPEPGYPVYANATILAGATPHFMPLEASNGYLPDLDRI
                     PPAVLNRARIIFLNYPNNPTGAAAPDRFWKEAIALAARYNIILASDAAYSEIYYGERP
                     KSILEFPGGREVGIEFHSFSKTFNMTGWRVGFAVGNRQALAGLAAVKENLDSGVFEPV
                     QAAAAAALAGPEAFTAGLRRMYRKRLETLIAGLNGYGLAARMPAGTFYLWVAIPAGRT
                     SAEYAGHLLEKKGILTTPGNGFGPHGEGYIRFSVTIDETRILEALERLKQG"
     gene            complement(4540..5328)
                     /gene="dapB"
                     /locus_tag="PKN80_03150"
     CDS             complement(4540..5328)
                     /gene="dapB"
                     /locus_tag="PKN80_03150"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNQ35043.1"
                     /translation="MSEQRIGIAVAGAAGRMGQVIIGLLKEHPRACLAAALERSGHPD
                     LGRPVGPDILITDDLAGALQKAAVLIDFTTPAATLEHLAAAKKAGRPIVIGTTGLSPE
                     EMETIRAAGREIALLASPNMSPGVNLLFRLTAEAASRLKDYQVEIVEAHHDRKVDAPS
                     GTALRLAEIVAADRNQDLKTAAVYGRSGRCGPRPDGEIGIHAIRVADVVGEHTVIFGA
                     AGERLELHHRATSRQTFATGAIRAALFLVDRKPGFYSMAEVLGI"
     gene            5434..6348
                     /locus_tag="PKN80_03155"
     CDS             5434..6348
                     /locus_tag="PKN80_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNQ35044.1"
                     /translation="MAADIANRLSDFLSAGRVLLADGAMGTRLSELGLPPGQAPESWN
                     LLNPAAIGQVHRDYLAAGVRVLLTNTFGANPFKLERYGLSDRLEEVNRRGVELARAAA
                     GSRPILVAGDLGPSGELLEPLGRLSAAALKAGYRRQVQGLESAGVDFFLIETMTSLDE
                     ARAISDAVAEVSARPLAVSFTFSVGRQGPRTLMGESPLQAAKAFGGRAWLLGANCGEG
                     PAETVQVLQELRAARPGQLLWAKPNAGRPVLRDGRSVFLQGPEDFGREALRLAQAGAA
                     VVGGCCGTTPAHLAAAARRLAHLNLLAK"
     gene            6463..7536
                     /gene="rpsB"
                     /locus_tag="PKN80_03160"
     CDS             6463..7536
                     /gene="rpsB"
                     /locus_tag="PKN80_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNQ35045.1"
                     /translation="MVGVTLQELLESGLYFGHPSKQFDARMKPFIFGKRQGIYIIDLE
                     KTRVCLEKACEFLKEMAREGKVIMFVGTKRQARELVRTTAETLDLPYMSNRWLGGTMT
                     NYQTLRVRIERLNTLEAKDTSGALDKLSKWDATMARRERDRLMKNLSGLRKLNRLPDI
                     LLVVDVRKEENAILEARKLGLKIVGVVDTNSNPELVDYPIPANDDGIKGLRILFAEFA
                     TAIKSGQEERGRLQKAAEEKEKKAAREAAAAAQKPPVPEAAPAVEPVAAAAPVAKPAA
                     KPAVPAAPAAEPAEAEAGSDESPAAGKKAVTKPKPRPRIRSKVRRSAPATAARKPAAR
                     SAGAARLEKKTTAGVEKKSKKEE"
     gene            7539..8126
                     /locus_tag="PKN80_03165"
     CDS             7539..8126
                     /locus_tag="PKN80_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNQ35046.1"
                     /translation="MAIDAKMIKDLREETGFGVMDCKRALEEAKGDLNKARALLKQRG
                     DEIVATKSGRVAREGIIASYVHTGGKIGVLVEINCETDFVARNEIFQAFARDICLQVT
                     ASNPTYLSRTEVPAAVIEEQKQMVATLSEGKPAAILEKIQAGKLEEFFKSCVLLDQPF
                     VKEPELSIQAYLDQTLARLGEKIAIRRFIRWELGG"
     gene            8192..9640
                     /locus_tag="PKN80_03170"
     CDS             8192..9640
                     /locus_tag="PKN80_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35047.1"
                     /translation="MPKKYQLQDRVSAQIFLENLAFTNQEFFFLAGYFLKVKKRVGRY
                     FLNYLRREAHEVETILDDAEVFTNELFFFRELMAGIRWFCRAAFTLEEGIISRYDTRK
                     LKDTPADRTALMAEAENTLNYFYTTIRKLCQEALRISADLGVVLPDKAAESHFIKIQG
                     QPRLEKNRAIDSGTTPLDSVELLTGEFIGAQESLLRFEARLREGKATLNERDVIQINN
                     SFHALESFYDTHLHDTLMERDVPDFWSLRGYIAMVLRFSEMLKDLVHFYERHLVTMRD
                     PAIHDRFAELVDEKRLLKILVDFVLKNSARYVKDGGSLALELLTRVTGKVRRVIKIPI
                     GVDGLHARPMTWIYKVCQRHGNVIFEVGGEKFEANSILSMLTMTEAIDRVIGLEDKDS
                     QIVVAAGTVINELERRRKPVSVTGRRYVPQTVIDILESFRSEIRDKSGTRFVDVVASG
                     PDASIREIEVLAAAYFKKEQLPPSLDYIFKED"
     gene            complement(9725..>10033)
                     /locus_tag="PKN80_03175"
     CDS             complement(9725..>10033)
                     /locus_tag="PKN80_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNQ35048.1"
                     /translation="VMRSLEAPIRQIAENSGKEGSVVTDYVRSHKEVGFNADKNTYED
                     LVKAGIVDPTKVVRSALQNAASIGALLLTTEVLVAEKPEEKKEPPMPAGGGGYGGGMY
                     "
BASE COUNT         1766 a         3206 c         3260 g         1801 t
ORIGIN      
        1 aaaaaagcaa ctccgcgacg gcgctcaccg aaggccgccg cttaaaggga aaggacgaaa
       61 ggcaaaggcg aggatgggca tcaaatgagg cgtccgtcga gatatttcaa tcggatttcc
      121 atgtgagact ttgggcgtga ggccgatgag aaagaagggc tggcatatcc cggaggagac
      181 catccgccgg ctttcgcttt acctgcgtac cctgcgttac ctggaaaagg agggggtgcg
      241 cgtcatctcc tccgaacgcc tcaccaagca ccttcacata accagcgccc aggcccgcaa
      301 ggacctggcc tacttcggct ccttcggcca tcccggggtc gggtacaatg tccgccgcct
      361 ccgcgagaag atcgaggaga tactcggcct ggaccgccac tgggacgtca tcctggtcgg
      421 cgcgggcaag atgggcgccg ccttcctggc ctacccgggc ttccgccgtt tcggtttcaa
      481 gattcgcgcc gccttcgaca acaatcccgc caagatcggt cagaagattg gcggcatccc
      541 ggtactggac gaccagcgga tggaggagtt tatccaggcg aaccggatcc gggcggccat
      601 catcaccgtg ccggccagcg ccgcccagga tgtagcccgg cggctggtcg gcagcggcat
      661 taccgccatc ctgaacttcg ccccccgcta cctctccatc cctgagcaca tccacatcaa
      721 gagcgtggac gtggccatgg agttggagaa tctggtctac taccttacta attcctgaac
      781 gacttcaggc gctcaggacg tgggcgagtc ggtaggccgc ccggtccagc ttcagccgct
      841 tccgggcctt cttcaggtca accaccacca ctttttcttt ttccagcccg acgcagacgg
      901 cctgcctgcc ctcggccagc gcctcgaccg cccgggcgcc gaaacggaga gccaggcagc
      961 ggctccgggc cgacggggcc cccccgcgct ggatgtagcc gagtaccgag taccggacct
     1021 cgcccgggta aaattcggcg atccggggtg cgagttgggc gacctcgacg gccccctcgg
     1081 ccaccaccat gatcatgctg cgctttccct tggcccggtc ggccagcagc aactcacgga
     1141 tcgcctccag gtcaaagggc acttcgggga ccaggatggc ctcggcgccg gcggccagac
     1201 ctacctccag ggccaggaag cccttctccc gtcccatgac ctcgaccagg aagatgcgtt
     1261 cgtggctggt ggcggtatcc cggatgcggt cgatggcctc cagggcggta ttgacggcgg
     1321 tgtcgaagcc gatggtctcg gtggtgccgg agatatcatt gtcgatgctg gccgggatga
     1381 aggccagcgg gtgtttccac tcggccacca gggtctggct ggccgccagc gaaccgtcgc
     1441 cgccgatgac caccaggctc ccgatgcctc tctgctcgag gttccggtag caggcccgcc
     1501 ggaagtccgc cttccggaaa cccgggcagc ggatggtctt gagcacggtg ccgcccaggt
     1561 tgatgatgcc gctgaccgag ccgtggccca tttcccggaa atctttttcg tagaggccct
     1621 ggaagccgcg ttcgatgccg actacctccc agccgaaggc cagggcgcgc cggacgaccg
     1681 cccggatggc cggattcatg cccggggcgt cgccgccggt ggtgagaagt ccgatccgcc
     1741 tggttttcgt catttaaaga agacccgggc caggtccagc caggccttgt cgaccacctt
     1801 caggttggcc aatgcctcgg ccaggggcac tccgatgacc tcctggccgc gcagggcggc
     1861 catcatgccg aacttccctt ccgaaaccag ctcggccgcc ttgactccga cccggaggcc
     1921 caggatccgg tcgaaaagag tgggcgggcc gccccgctgc atgtgtccga tcaccgccga
     1981 acgggtctcg acgccggtct ccttttcgat gtaatcggcg atcttttcgc ccagattccg
     2041 cttcttgagg atgatgtgtc cgaaggcatc cttctcgccc agcggctcct ccccgtcctc
     2101 gctgaactcg gttccctccg agacgaccac cagggccacc ttcttgcggg cgaagacctg
     2161 tttcaggtgg gcggccatgg ccgcgtagtc gggtttgaac tcgggcagca gcgcccagtc
     2221 ggcgccggcg ccgagggcgg tgtagagggc cacccagccg gcgtggcgtc ccatcacctc
     2281 caggaccatg atgcggcggt ggctggtgcc ggtgtccagc agggcccggc aggcatccag
     2341 cgcccgggtg accgaggtgt cgaagccgaa ggtgtaatcg gtcgcggaaa ggtcgttgtc
     2401 catggtcttg ggtacgccga ccaccttgac cccgcgctgg tgcagcttgt tggccacgcc
     2461 cagggtatcc tcgccgccca tggccaccag ggcgtcaatg cccagtttct tgagattcgc
     2521 ttccacctgc tccggggccc cttccttctt gaagggattg aagcggctgg tgcccaggat
     2581 ggtgccgccg cggtccacca gcggctcgac ctccttgagt ccgagcggga tggtgacggc
     2641 gttgtagggt cccttccagc cgtccaggaa tcccacgcac tcgtagccga aatccagcgc
     2701 ccggtagacg cagccccgga tggccgggtt caagcccggg cagtcgccgc cgccggtcag
     2761 aatgccgatc ttcattgcct gcctcccttg attggtttga gagtattgtc tggttctttt
     2821 ttgaggttgt cgatccgccc ggccggcgcg gccggacatg ttttttatta tttaacgccg
     2881 aaccttccgc ccggaccggg tcgcgacgcc gggcgcggcg agcgccagaa ttcttgcggc
     2941 cagcagggcg gcgttctggg gtccttggct tcccagcccc agcgtggcca ccggtatccc
     3001 gcgcggcatc tgcaggatgg ccagcagggc gtccagcccg ttaagcggtc cgaccgccag
     3061 cggcacgccc agtaccggca gagttgtgtg gctggcgatg aacccgggca gggccgccga
     3121 aagacctgcg gcggcaatga ttacccggac gccacggccg gccgcctgct gggcgaaggc
     3181 ggccgtctca tccggctgcc ggtgggccga gagcaccttg acttcgtaat cgattccgga
     3241 ggccttgagc gtctcctcgg ccggggccag cgccggcaga tcggacttgc tccccactac
     3301 gatgaccact tccgccatga tcgcctcatc cttgcttcag gcgctccagg gcttccagga
     3361 tgcgggtttc atcgatggtg acggagaacc tgatgtaccc ctcgccgtga ggcccgaagc
     3421 cgtttccggg cgtggtgagg atgccttttt tttcgagcag gtggccggcg tactcggccg
     3481 aggtgcgtcc ggccggtatg gccacccaga gataaaaagt gccggccggc atccgggccg
     3541 ccagcccgta gccgttcaat ccggcgatca gggtttccag gcgtttccgg tacatccggc
     3601 gcagtccggc ggtgaacgcc tccgggccgg ccagggcggc tgcggcggcg gcctggaccg
     3661 gttcgaagac cccggagtcg aggttttcct tgaccgcggc caggccggcc agggcctggc
     3721 ggttgccgac cgcgaacccg acccgccagc cggtcatgtt gaaggtcttg gagaaggagt
     3781 ggaactcgat cccgacttcc cgcccgcccg gaaattcgag gatgcttttg ggcctttcgc
     3841 cgtagtatat ctcggagtag gcggcgtcgg aggccaggat gatgttgtac ctggccgcca
     3901 gcgcgattgc ctccttccaa aagcggtccg gggcggcggc gccggtgggg ttgttcgggt
     3961 agttgagaaa gatgatccgg gcccggttca gcaccgcggg cggtatccgg tcgaggtccg
     4021 gcaggtagcc gttggatgcc tccaggggca tgaagtgggg cgtggcgccg gccaggatgg
     4081 tggcgttggc gtaaacggga tagcccggtt cgggcaccag gcagatatcg tcccggtcca
     4141 ggaaggcgaa gggcaggtgg ccgattccct ccttggagcc gatcaggaaa aggacctcgc
     4201 cagccggatc cagctcaacc ccgaagcgcc cccggaacca ggcggcgatt gcctctcgga
     4261 agaccggctt tccgcggccg agcgggtaac gatggccggc cgggttggca accgattcct
     4321 gcattttgcc gaccacgaaa tccggggtgg gcgtgtccgg gtcgccgatt ccgaaatcga
     4381 tgatgtcgac tccggcctgg cgcagctcgg ccttgcggcg gtcgatttca atgaagagat
     4441 aaggcggcag ccggttaaga cgctcgttga acctgatgtc catgtttctt tccggttggc
     4501 ggtggggtgg ttccgtttga atgctttgcc cgatccgctt cagataccga gtacttcggc
     4561 catggagtag aagccgggct tccggtcgac caggaaaagc gccgcccgga tggccccggt
     4621 ggcgaaggtc tggcgggagg tggcccggtg gtgcagttcc aggcgttccc cggcggcgcc
     4681 gaagatgacc gtgtgttcgc cgaccacgtc ggcgacccgg atggcatgga taccgatctc
     4741 gccgtccggg cgagggccgc accggccgct gcggccatag acggccgcgg tcttgagatc
     4801 ctgattgcgg tcggcggcca cgatttcggc cagccggagg gcggtgccgc tgggcgcgtc
     4861 cacttttcgg tcgtggtgcg cctcgacgat ctcgacctgg tagtccttaa gccggctggc
     4921 cgcctcggcg gtcagccgaa aaaggagatt cacgcccggg ctcatgttcg gggaggcgag
     4981 gagggcgatt tcccgtcccg ccgcccggat cgtctccatc tcctccgggg ataagccggt
     5041 ggtcccgatg acgatcggcc ggccggcctt tttggccgcc gccaggtgtt ccagggtggc
     5101 cgccggtgtg gtgaagtcaa tcaggacggc cgccttttgg agggcgccgg ccaggtcgtc
     5161 ggtaatcaaa atgtccggcc cgaccggccg gcccaggtcc gggtggccgg atctctccag
     5221 ggcggcggcc aggcaggccc gcgggtgctc cttcagcagg ccgatgataa cctggcccat
     5281 gcggccggcg gctccggcca cggcgatacc gattctttgc tcagacatga acaggcataa
     5341 ttttactcca gaaaaaccac tttcgtcaaa gttgcggatc ggctataata aacaattgaa
     5401 ccttccggca cgccttttaa cctggagaga aaagtggctg cggatattgc gaaccggctg
     5461 tccgatttct tgagcgcggg ccgggttctc ctggccgacg gggccatggg gacccgcctg
     5521 tccgagctgg ggttgccgcc cgggcaggct ccggagagct ggaaccttct caatccggcg
     5581 gccattggcc aggtgcaccg ggattatttg gcggccgggg tccgggtcct gctgaccaac
     5641 acctttgggg ccaacccctt caagctggaa cgttacggac tttcggaccg gctcgaagag
     5701 gttaaccgcc ggggcgtgga actggcccgg gcggccgccg gttcccggcc gatcctggtg
     5761 gccggcgacc tgggcccgag cggagaactg ctggagccgc tgggccgtct ttccgcagcc
     5821 gccctcaagg ccggctaccg ccgccaggtg caggggctgg agtcggccgg ggtcgatttc
     5881 ttcctgatcg aaacgatgac ctccctggac gaagcccgcg cgatttccga tgcggtcgcc
     5941 gaggtgagcg cacgccccct ggcggtctcc ttcaccttct cggttggccg gcagggtccg
     6001 cgcaccctga tgggggagag cccgcttcag gcggccaaag ccttcggcgg ccgggcctgg
     6061 ctgctggggg ccaattgcgg cgaaggaccg gccgagaccg tccaggtgtt gcaggaactg
     6121 agggcggccc ggcccggcca gctgctctgg gccaagccca acgccggccg gccggttctg
     6181 cgcgacggcc gaagcgtctt tctccagggt ccggaggatt ttggccggga ggctctgcgg
     6241 ctggctcagg ccggggcggc cgtggtcggc ggctgctgcg ggaccactcc ggcccatctg
     6301 gccgcggccg cccggaggct ggcccacttg aatttactgg cgaaatagta taaaataact
     6361 gtttaaattg tattttcaaa accggggaaa gggcccggcc ggttgccaac cggcagcctt
     6421 ttcccagatc cgaaccttaa aagataaagc caggaggtaa ctttggtcgg ggtaacactg
     6481 caggaactgc tggagagcgg tctctatttc ggccatccga gcaagcagtt cgacgcccgg
     6541 atgaaaccct ttattttcgg gaagcgtcag ggaatttaca tcattgatct cgagaagacc
     6601 agggtctgcc ttgaaaaggc ctgcgagttc ctcaaggaga tggcccggga gggcaaggta
     6661 atcatgttcg tgggcaccaa gcgccaggcg cgcgaactgg tgcgcaccac cgccgaaacg
     6721 ctggacctgc cttacatgtc gaaccgctgg ctgggcggca ccatgaccaa ttaccagacg
     6781 ctgcgggttc gcattgaacg gctgaatacc ctggaggcca aggatacctc aggcgccctg
     6841 gacaagcttt ccaagtggga tgccaccatg gcccggcgcg aacgggaccg tctgatgaag
     6901 aatctttcgg gtctgcggaa actgaaccgc ctgcccgaca tactcctggt ggtggatgtc
     6961 cggaaggagg agaacgccat ccttgaggca cggaaactcg gcctcaagat cgtcggcgtg
     7021 gtggatacca actccaatcc ggaactggtt gattacccga ttccggccaa cgacgacggc
     7081 atcaagggcc tgcggattct ctttgccgag ttcgcgaccg ccatcaagtc cggccaggag
     7141 gagcggggcc ggcttcagaa ggcggccgaa gagaaggaga agaaggcggc ccgcgaggcg
     7201 gccgcggccg cccagaaacc gcccgttccg gaggcggccc cggccgttga accggttgcg
     7261 gccgccgctc cggtcgccaa accggccgcc aaaccggccg tacccgccgc tccggccgcc
     7321 gaaccggccg aggccgaagc cggttcagac gagagtccgg ccgccggcaa gaaggcggtt
     7381 accaagccaa agccccggcc gcgcatcagg tcgaaggtgc gccgatcggc cccggccaca
     7441 gccgccagga aaccggccgc ccgttccgcc ggcgccgccc gtctcgaaaa gaagacgacg
     7501 gccggcgttg aaaagaagag caaaaaagag gagtaatgat ggcgatagac gcgaagatga
     7561 tcaaggacct ccgggaggag accggcttcg gagtgatgga ttgcaagcgg gcactcgaag
     7621 aggccaaggg tgatttgaac aaggcgcgcg ccctgttgaa gcagcgcggc gacgagattg
     7681 tggccaccaa aagcggccgg gtcgcccggg agggcattat cgccagttac gtccataccg
     7741 gcggcaagat cggcgtcctg gtggagatca actgcgagac cgatttcgtg gcccggaacg
     7801 agattttcca ggccttcgcc cgggacatct gcctgcaggt gacggccagc aacccgacct
     7861 atctttcccg caccgaggtg ccggcggcgg tgatcgagga gcagaagcag atggtggcca
     7921 ccctttccga gggcaagccg gccgcgatac tggagaagat ccaggccgga aaactcgagg
     7981 agttcttcaa gagttgcgtg ctgcttgacc agccgttcgt aaaggaaccg gaactgagca
     8041 tccaggccta tctcgaccag accctggccc ggctcggaga aaaaatcgcc atccggcgtt
     8101 ttatccgctg ggaactgggc ggttgaccgc tcggagcggc cggtccggtc cgggcctcct
     8161 gccggaagag gccggcggcg gcgcttccgc catgcccaag aaatatcagc ttcaagaccg
     8221 agtttcggcc cagatcttcc tggaaaacct ggcctttacc aaccaggagt tcttctttct
     8281 ggccgggtac ttcctcaagg taaagaagcg ggtcggccgt tacttcctca actacctccg
     8341 ccgcgaagcc cacgaggtgg agaccatcct cgacgacgcc gaggtcttta ccaacgagct
     8401 cttcttcttc cgggagttga tggccggcat ccgctggttc tgccgggccg ccttcaccct
     8461 ggaagagggg atcatcagcc gctacgacac ccgcaaactc aaagacaccc cggccgaccg
     8521 gacggccctg atggccgagg ccgaaaacac cctcaattac ttttatacca ccatccgcaa
     8581 actctgccag gaggccctcc ggatcagcgc cgacctgggc gtggtcctgc ccgacaaggc
     8641 ggccgagagc cacttcatca agatccaggg gcaacctcgg ctggagaaga accgggcgat
     8701 cgattccggc accactccgt tggactcggt cgaactgctg accggtgaat tcatcggcgc
     8761 ccaggagtcg cttctccgct tcgaggcgcg cctccgcgaa ggcaaggcca cccttaacga
     8821 gcgggacgtg attcagatca acaacagttt ccacgccctg gagagtttct acgacaccca
     8881 cctgcacgac acgctcatgg agcgcgacgt tcctgatttc tggagcctgc gcggctacat
     8941 cgccatggtg ctgcgtttct cggagatgct gaaggatctg gttcacttct acgagcgcca
     9001 cctggttacc atgcgcgatc ccgccatcca cgatcgtttt gccgaactgg tcgacgagaa
     9061 gcggctgttg aaaatcctgg ttgattttgt cctcaagaat tcggcccgat acgttaagga
     9121 tggcggctct ctggccctgg aacttctcac ccgggtgacc ggtaaggtgc gccgggtcat
     9181 caagataccg atcggcgtcg acggcctcca cgcccggcca atgacctgga tctacaaggt
     9241 ctgccagcgc cacggcaacg ttatcttcga ggttggcggg gaaaagttcg aggccaacag
     9301 cattctctcg atgcttacca tgaccgaggc gatcgaccgg gtcatcggcc tggaggacaa
     9361 ggacagtcag atcgtggttg ccgccggtac cgtcatcaac gaactggagc ggcgccggaa
     9421 gccggtctcc gtgaccggcc gccgttacgt accgcagacc gtgattgaca tcctggagtc
     9481 cttccggtcc gaaatccggg acaagtccgg cacccgcttc gtcgacgtgg tggccagcgg
     9541 tccggacgcc tccatccggg agatcgaggt cctggccgcc gcttacttca aaaaggagca
     9601 gctgccgccc tccctggact acatcttcaa ggaagattga aattttattg gtgtggttcc
     9661 ggccgtcaat aaaaaaggcg gggttcgcgc gaaccccgcc ttttttattg gcttggacgt
     9721 tcgatcaata catcccgccg ccgtaaccgc cgccgccggc cggcatcggg ggttccttct
     9781 tctcctcggg cttctcggcc accaggacct cggtggtcag gaggagggcg ccgatactgg
     9841 cggcgttctg gagggccgaa cggacgacct tggtcggatc gacgatcccg gccttcacca
     9901 ggtcttcgta ggtgttcttg tccgcgttga aaccgacttc cttgtggctc cggacatagt
     9961 cggtgaccac cgagccttcc ttgccgctgt tttcggcgat ctggcggatg ggggcttcca
    10021 gggagcgcat cac
//
LOCUS       DAOQYN010000049         9856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_7359_length_9856_cov_5.090399, whole genome shotgun sequence.
ACCESSION   DAOQYN010000049 DAOQYN010000000
VERSION     DAOQYN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7359_length_9856_cov_5.090399"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1294
                     /locus_tag="PKN80_03180"
     CDS             119..1294
                     /locus_tag="PKN80_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ35049.1"
                     /translation="MKDPLSSRISNLPPYVFARINQLKAQARAAGKDIIDLGMGNPDL
                     PTPPHIVEKLTEVAHDPKAHRYSASQGIRGLRRAIVDWYAKRFGADLDPDREVVAVIG
                     SKEGLCHFALAAFEPGEAVLVPNPTYPIHLYSFAIAGVGTVDLPLLPENGFLPDLGRI
                     NLKAGPRPKALLLSFPHNPTTSMVGLDYLAEAVDFCRRNDLFLIHDLAYGELVFDGRT
                     APSVLQVPGAKEVAIEFYSLSKTYCMAGWRVGFAVGNPQLVGALIRLKSYYDYGIFTP
                     IQVAAAAALRGPQDCVVQARETYQARRDLLVDRLARAGWEVPRPPATMYIWAPLPEKF
                     RAMGSMDFAAFLLERAEVVVSPGVGFGRYGERYVRLALVENEQRLRQAARNIAKALQ"
     gene            1291..2448
                     /locus_tag="PKN80_03185"
     CDS             1291..2448
                     /locus_tag="PKN80_03185"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNQ35050.1"
                     /translation="MKPRLSERVRNLEPSATLAVGRQARELQARGVKVLNLSVGEPDF
                     PPPEAAKEAVLGAVRENFSRYTESRGLPELRAAVARRAETDLGLKYRPEQVVVSCGSK
                     HTLYNLFQVLLDPGDEVLIPVPYWTSYPEMVKLAGGVPVPVEAGGGFRVRPADLEARR
                     TARTRALILNSPNNPSGEVYPAEELAGIADWLVRNDLLVISDDAYQHFLYDGRTFTSI
                     ASFPGLPERTLVVGAASKTYAMTGWRIGWFLGPEPIAEAVARLQDQSTSNPSSLSQRA
                     ALAALDGGQEPVFRMREAFQRRRDALMKGLAAVKSIRYPFPAGAFYLFFTHPSITDSV
                     ETAGRILAEKQVALVPGRAFGMEPYLRLSYAVADQVLEEAVRRLVDFFRAA"
     gene            2474..3304
                     /locus_tag="PKN80_03190"
     CDS             2474..3304
                     /locus_tag="PKN80_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35051.1"
                     /translation="MRALRRASGLAGLLALLLLSVPLRAEAPILLRYRFTPGQVLRYG
                     MEVRANARLGSTALPPYVLEQDYGIELEVLSVEPNGAARIEIRQKLQRNRVLLSRQEL
                     PVAAPEVSTTRFQLRVEPNGSGFVPLGSSIPGSQTAAAAWLLFPAFPERPMAAGHSWT
                     TIHETALSVAGGSVSSRYNARYTYDGPDRQTVPGSARVAAEVKLRIPTPGHADKQRPA
                     TITGSGEGHGCLYFREGRLVYGTLEGTSATDSRLPLAGGAGFSLPLDTTFTARLRLIS
                     "
     gene            3314..4276
                     /locus_tag="PKN80_03195"
     CDS             3314..4276
                     /locus_tag="PKN80_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ35052.1"
                     /translation="MLRYAGRRLLHLVPLLLGMTFISFLVMQLAPGDYFTRLSLDPQV
                     SAETLAALRRQFGLDASPVIQYLKWLRNLATLNLGVSFVYHIPVATLIRQRLFNTLQL
                     SLATMLLTWLISVPLGILAANRAGRTADRALSAAAFVSISLPSFFLALLALVWAARTG
                     FFPLGGSESIFAAELGPGLRVLDRLWHLALPALVLTAAGFGGLFRVARANYREALSAP
                     FVTALRGRGLPESRVRVHVLRNSLNPLITLLGYELSGLISGVALVEVILAWPGLGQTM
                     LTAVMSQDLYVVMAALFFGGLLLIAGNLAADLLLALNDPRIRLD"
     gene            complement(4296..5423)
                     /locus_tag="PKN80_03200"
     CDS             complement(4296..5423)
                     /locus_tag="PKN80_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNQ35053.1"
                     /translation="MKYRDLGRTGLKVSRLGLGTMRLPMADGRVQVEEAEAVVRRALE
                     LGVNYFDAAVFYLKDESESLLGRCLKGWKKPVVISTKNHYQGDSGPDWRRNLDQSLRR
                     LEVPAIDCYHIHALSYEAFRGPMSVPGGPWDEIRRARKEGLIRHFGFSCHDTPANMEE
                     LVKTGEFELVTLQYNLLFPQNAPVIKLAAKRGLGVIVMGPVAGGRLACPSESVARLLP
                     DAASNAEIALRFVLDNPDVTVALSGMYSVAQVEENVRTAGRGEPLTPGERKQMLEMLN
                     RFQRLADLYCTGCGYCLPCPHGVAIPVNFEAYILADIFGLQEGAEAQYRRLQQRTDNE
                     GRPTPASADACRDCGLCEPRCPQRIPIRARLKETHRRLGRG"
     gene            5505..6470
                     /locus_tag="PKN80_03205"
     CDS             5505..6470
                     /locus_tag="PKN80_03205"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNQ35054.1"
                     /translation="MKTAIFGFSGAGKTALSARLAGKRVESFDPYQPAALAVKLADRR
                     LARLREIVKPAKLTPPEVLLLDFKGPARAAGIEEKVLAGLLEADVIILVLDGFSAGAN
                     PAADLESLLLEFVFRDADRVASIIERRRTEQAAGRRRANPVEDEALKRAAEHLAGERP
                     LYRLERGPADAAFLAALGLISDRKFFLLLNGPSADPALAGAAERLQLDAFRLDLAGDG
                     PPDEAVAEFWATFLDRVGLITFYTAGEKETRAWLLPRGATALAAAGQIHTDIARGFIR
                     AEVVNFTEFDRLGGYGACRQHGALRLEGKEYPVVDGDLLHIRCST"
     gene            6543..7415
                     /locus_tag="PKN80_03210"
     CDS             6543..7415
                     /locus_tag="PKN80_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ35055.1"
                     /translation="MDKTRNRLIGGCLLLAMLLWFGSRLSAADPDPAETARVKAVFAL
                     LRTALAQHDSKGVYEQLGQERNLAQSLENYQEMIQIRRLRGGWIRPDLEYDPERLEVE
                     FLPDEDGRGRARVWKLNGDGKRAGSEEFAREGKNWKWLPASGYLWFPEPSGELEEIER
                     RRQAMEAGLASPNPFMRFQTAVNLLEHKQSKTRALQVLQNLLKEPDPRFRLRVAEAVL
                     GAGDKNAVPVLIDLLAISDTYARAKFLLEKSTGQNFGALTGITTKAEVTKFLNDWKAW
                     WNQNKGSFQLPAAP"
     gene            7505..9802
                     /gene="bamA"
                     /locus_tag="PKN80_03215"
     CDS             7505..9802
                     /gene="bamA"
                     /locus_tag="PKN80_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNQ35056.1"
                     /translation="MFYFIRRPFLFFLTLAIGLAPLLSGTARAQEEKVVRAVEVAGNQ
                     AVSTGRVLLLLQTRAGEPYRERTLAEDVSRLLSTGFFAEVVPRTEPTSDGGLRVIFQV
                     RENPTLARVTFSGNRKLRKEVLLERSGLAAGQIYEAARVAEAAERIRDFYREKGLAFT
                     TVEPKAELAGENRMGVVFLIREAAAGRISAIGFTGNQFYKREKLLRVLQLKERSLVKF
                     KLGTFNEKAFQDDQARIRALYRQAGFADVKVAGATRPAGRYLEVDFSIEEGRRYLAGP
                     VNFSGDLLYTPEKLRDQVRMKEGVPYSQAGLDADRSSLLNLYRDDGYLKARVEPVAVF
                     NTASERVDIGYQVDPGPVVEVEGVEVSGNTRTRAGVIRREIKLAPGDRFDGRKLRRSI
                     ENLRDLNYFEDVNIQPEEGSDPGKAVLNFDVTERERTGNLMFGAGYSSVDGLIGLLAV
                     EQTNFDWHNPPSFIGGGQTLRLSSDFGRQRQGFSLGFTNPYIYDRPVLFGFDLYDRDR
                     EYDEFDERRTGGDLRFGRRVNEYWSVSAMPRAERVDVSDISPFASTEYLKEEGENDIY
                     SLTLAAARDTRDSRLRTTRGTRQELSYKYAGGWLGGDRDFWRGDYDFTYYRKLGPAWT
                     FSSQTRLGIADTMGSTSDVPVYERYFCGGARTVRGYEDRSLGPLDASGLAKGGNFTLI
                     QNLEFSRPIYQDILHLVLFADSGQAWWRANDFDFSELKSSLGAGLRLKVPIFPLPIKL
                     DYGYALDPLPGEDSGRWHFLIDWWF"
BASE COUNT         1542 a         3266 c         3365 g         1683 t
ORIGIN      
        1 tcaacctgcc ggcgccggcc ggcggtcggc tgaccgtcat caaggccggc gagggcgaaa
       61 aggttgcggt gggcgcgacc ctggccgaaa tagaataaaa caaacgctgc ggggaaaaat
      121 gaaagatcct ttatccagcc ggatttccaa cctgccccct tacgtctttg cgcgcatcaa
      181 ccagctcaag gcgcaggccc gggcggccgg aaaggacatc attgacctgg gcatgggcaa
      241 tccggatttg ccgacgccgc cccatatcgt ggagaagctg acggaggtgg cgcacgaccc
      301 gaaggcgcac cggtattcgg cctcgcaggg tatccggggc ctgcgccggg cgatcgtcga
      361 ctggtacgcg aaacgcttcg gcgccgacct ggatcccgac cgcgaggtgg tggccgtgat
      421 cggcagcaag gagggtctct gccactttgc cctggccgct tttgagccgg gcgaggcggt
      481 cctggtgccc aaccccacct accccattca cctttactcc ttcgcgatcg ccggggtcgg
      541 aacggtcgac ctgccgttgc tgccggagaa cggttttctg ccggacctgg gccggataaa
      601 cctgaaggcc gggccgcggc ccaaggcgct gctgctcagc tttccccaca acccgaccac
      661 cagcatggtg gggctggact acctcgccga ggcggtcgat ttctgccgcc ggaacgacct
      721 cttcctgatt cacgacctgg cttacggcga gctggttttc gacggccgga ccgcccccag
      781 cgtcctgcag gtgcccgggg ccaaggaagt ggccatcgag ttctactccc tctccaagac
      841 ctactgcatg gccggctggc gggtgggctt cgcggtgggc aatccgcagc tggtgggcgc
      901 gctcatccgg ctcaagagtt actacgacta cggcatcttc accccgattc aggtggcggc
      961 ggccgccgcc ctgcgcggtc cccaggactg cgtggtccag gcccgggaga cctaccaggc
     1021 gcgccgcgac ctgctggtgg accggctggc ccgggccggc tgggaagtac cgcggccgcc
     1081 ggcgaccatg tacatctggg cgccgctgcc ggagaagttc cgggccatgg gttcgatgga
     1141 cttcgcggct ttcctgctgg aacgggccga ggtggttgtt tccccggggg tggggttcgg
     1201 ccgctacggc gagagatacg tgcggctggc cctggtggag aacgagcagc ggctgcgcca
     1261 ggcggcccgg aatattgcga aggcgctgca atgaagccgc ggctttccga gcgggtccgc
     1321 aacctcgaac cttcggcgac cctggccgtc gggcgccagg cccgggaact ccaggcccgg
     1381 ggtgtcaagg tgcttaacct ctcggtgggc gaaccggatt ttccgccgcc ggaggcggcc
     1441 aaggaggcgg tgctgggcgc ggtgcgggag aacttttcgc gctataccga aagccgcggc
     1501 ctgcccgaac tgcgggccgc cgttgcccgc cgggccgaaa ccgatctggg cctgaagtac
     1561 cggccggagc aggtggttgt ctcctgcggt tccaagcata cgctttataa ccttttccag
     1621 gttctgctgg acccgggcga cgaggtgctg atcccggtgc cctactggac gagttacccg
     1681 gagatggtga aactggccgg gggcgtcccg gtgccggtcg aagccggcgg cgggttccgg
     1741 gtgcggccgg cggacctcga ggcgcgccgg acggcgcgca cccgggccct gatcctgaac
     1801 agcccgaaca acccgtccgg cgaggtttac ccggccgagg agctggccgg gatcgccgac
     1861 tggctggtcc ggaacgacct gctggtcatt tccgacgacg cctaccagca cttcctttac
     1921 gacggccgca cctttaccag catcgcctcc tttcccggcc tcccggagcg gacactggtg
     1981 gtgggggccg cttccaagac ctacgccatg accggctggc gcatcggctg gttcctgggc
     2041 ccggagccga tcgccgaggc ggtggcccgg cttcaggacc agtccacctc gaacccgagt
     2101 tcgctctccc agcgggcggc actggccgcc ctggacggcg gccaggagcc ggtcttccgg
     2161 atgcgggagg ccttccagcg gcgccgggac gccctcatga aagggctggc cgcggtcaag
     2221 tcgatccgat acccttttcc ggccggggcc ttttaccttt tcttcaccca cccctcgatc
     2281 accgattcgg tcgagacggc gggccggatc ctggccgaga agcaggttgc cctggtcccc
     2341 ggccgcgcct tcgggatgga gccgtacctg cgcctctctt acgcggtggc tgaccaggtg
     2401 cttgaggagg cggtccggcg gctggtggat ttcttccggg ccgcctgagc cgaacccggg
     2461 tctgaggata aaaatgcgcg cactgagacg ggcgtccgga ctggccggcc tgctggcctt
     2521 gctgctgctg tcggttccgc tgcgggccga ggcgccgatc ctgctccgct accggttcac
     2581 cccggggcag gtcctgcgct acggcatgga ggtgcgggcc aacgcccggc tgggttcgac
     2641 cgccctgccg ccctacgtgc tggagcagga ttacggaatc gaactggagg tcctctcggt
     2701 ggaaccgaac ggagcggccc ggatcgagat ccgtcagaag ctgcagcgaa accgggttct
     2761 gctcagccgc caggagctcc cggtggccgc gcccgaggtt tcgacgaccc ggttccagct
     2821 tcgggtcgaa ccgaacggga gcggttttgt gcccctgggc agctcgatcc cgggttccca
     2881 gacggcggcg gccgcctggc tgctcttccc ggcctttccc gagcggccga tggcggccgg
     2941 gcattcctgg accaccattc acgaaaccgc cctgtcggtg gcgggcggat cggtgtcctc
     3001 ccgttacaac gcgcgttata cttacgacgg cccggatcgg cagacggtgc ccggcagcgc
     3061 ccgggtggcc gccgaagtga agttgcggat accgaccccc ggccacgccg ataaacagcg
     3121 gccggcgacg ataaccggtt ccggcgaagg ccacggctgc ctgtattttc gagaggggcg
     3181 gctggtctac gggacgctgg aaggaaccag cgccaccgac tcccggctgc cgctggccgg
     3241 cggcgccggc ttttccctgc cgctggacac cacctttacc gcccggctgc ggctgatctc
     3301 ctgaggccgg aagatgcttc gttacgcggg ccggcgcttg ctgcacctgg tgccgctcct
     3361 gctgggcatg acctttatct ccttcctggt catgcagctg gccccgggcg attacttcac
     3421 ccgcctgtcg ctggatcccc aggtttcggc ggaaaccctg gccgcactgc ggcgccagtt
     3481 cggcctggac gccagcccgg tgatccagta cctgaaatgg ctccggaatc tggccaccct
     3541 gaacctcggg gtttccttcg tttaccacat cccggtggcc accctcatcc gccagcgcct
     3601 cttcaacacc ctccagcttt ccctggccac gatgctgctt acctggctga tcagcgtgcc
     3661 gctgggcatt ctggccgcca accgggccgg ccggaccgcg gaccgggcgc tttcggcggc
     3721 cgccttcgtc agcatctcgc tgccctcctt cttcctggcc ctgctggccc tggtctgggc
     3781 ggcccggacc ggcttctttc cgctgggcgg cagcgaatcc atcttcgcgg cggaactggg
     3841 gccgggtctg cgggtgctgg atcggctctg gcacctggcg ctgccggccc tggtccttac
     3901 ggcggccggg ttcggcggcc tcttccgggt ggcccgcgcc aattaccgcg aggccctttc
     3961 ggccccgttc gtgaccgccc tccgggggcg ggggctgccg gagtcgcggg tgcgggttca
     4021 cgttctgcgc aacagcctga acccgctgat cacgctgctg ggatacgaac tttcggggct
     4081 tatttcgggg gtggccctgg tggaggttat cctggcctgg cccgggctgg gccagacgat
     4141 gcttaccgcg gtgatgagcc aggatcttta cgtggtgatg gccgcccttt tcttcggcgg
     4201 cctgctgctg atcgcgggaa acctggcggc cgacctgctc ctggcgctga acgacccgcg
     4261 catccggctc gactgaacgc cggcgggcgc cggcatcagc cccggccgag gcggcggtgg
     4321 gtttccttca ggcgggctcg gatggggatc cgctgggggc agcggggttc gcagaggccg
     4381 cagtcccggc aggcgtcggc gctggccggg gtcggccggc cctcgttgtc ggtgcgctgc
     4441 tggagccggc ggtactgggc ctcggcccct tcctggaggc cgaagatgtc ggccaggatg
     4501 taggcttcga agttgaccgg aatcgccacc ccgtgcggac agggaaggca gtaaccgcag
     4561 ccggtacagt aaaggtcggc cagccgctgg aagcggttga gcatttccag catctgcttc
     4621 cgttccccgg gcgtcagcgg ttcgccgcgc ccggcggtcc gaacgttctc ctcgacctgg
     4681 gccaccgagt acattccgga gagggccacg gtgacgtcgg ggttgtcaag cacgaaccgg
     4741 agggcgattt cggcattgct ggccgcgtcg ggaaggagcc gggccaccga ctccgaagga
     4801 caggccagcc ggccgccggc caccggaccc atgacgatga cacccaggcc ccgtttggcg
     4861 gccagtttaa tcaccggggc gttctgggga aagagcaggt tgtactggag ggtgacaagc
     4921 tcgaactcgc cggttttgac caactcttcc atgttggccg gcgtgtcgtg gcaggagaag
     4981 ccgaagtggc ggatcagccc ttccttgcgg gcgcggcgga tttcgtccca ggggccgccc
     5041 gggacggaca tcgggccccg gaaggcttcg tagctcaggg cgtggatatg atagcagtcg
     5101 atcgccggaa cctcgagccg gcggagcgac tggtcgaggt tacggcgcca gtccggtccg
     5161 gagtcgcctt ggtaatggtt cttggttgaa atgaccaccg gtttcttcca ccctttcagg
     5221 cagcggccga gcagggattc gctctcgtcc ttcaggtaga aaacggccgc gtcaaagtaa
     5281 ttcacgccca gctccagggc gcgccttacc accgcttcgg cctcttccac ctgaacccgg
     5341 ccgtcggcca tgggcaggcg catcgtgccc aggcccagcc gcgacacctt aaggccggtc
     5401 cttcccagat cccggtattt catagaaaaa ttttaccacg ggtgtcaaga tccggaccgc
     5461 gccagtgttg acaccgaagg aatagatggc tatcatagtt aacgatgaag acggcaatct
     5521 tcggtttttc cggcgccggc aagacggccc tctcggcgcg gctggccggc aagcgggtcg
     5581 agagtttcga cccttaccag ccggccgccc tggcggtgaa actggccgac cggcgcctgg
     5641 caagactgcg cgagatcgtg aagccggcca agctgacccc gccggaagtc ctgctgctgg
     5701 atttcaaggg accggcccgg gccgccggca ttgaggagaa ggtcttggcc ggcctgcttg
     5761 aggccgacgt gatcatcctg gtcctggatg gtttttcggc cggggccaat ccggccgccg
     5821 acctggagtc gctgctgctg gaatttgtct tccgcgacgc cgatcgggtc gcctccatca
     5881 tcgagcggcg ccggaccgaa caggccgccg gccgccggcg ggccaacccg gtcgaggatg
     5941 aggccctgaa gcgggccgcc gaacacctgg ccggggaacg gccgctttac cggctggaaa
     6001 gggggccggc cgacgccgcc ttcctggcgg cgctgggcct gatcagcgac cggaagtttt
     6061 tcctgttgct caacggcccg tccgctgatc ccgccctggc cggtgcggcc gaacggcttc
     6121 agctggacgc cttccggctc gacctggccg gagacggtcc gccggacgag gcggtggccg
     6181 agttctgggc gaccttcctg gaccgggtcg gcctgatcac tttttacacc gccggcgaga
     6241 aggagacccg ggcctggctg ctgccccgcg gggcgacggc cctggccgcg gccgggcaga
     6301 tacacaccga catcgcccgg ggtttcattc gggccgaggt ggtcaacttc acggagtttg
     6361 accggctggg cggttacggc gcctgccgcc agcacggcgc gctccggctg gaaggcaagg
     6421 agtatccggt ggtcgacggg gacctgctcc atatccgctg ctcaacctga gaccggcccg
     6481 gccggcttcg ataaacgccc ggccggcccg gaagaacaga atagacggcg ggaggaacga
     6541 aaatggacaa aacaagaaac cggctgatcg gaggctgcct tctcctggcg atgctcctct
     6601 ggttcgggtc ccggctttcg gccgccgacc cggatccggc cgagacggcc cgggtcaagg
     6661 cggttttcgc cctgctccgg acggccctgg cccagcacga ttccaagggc gtctatgaac
     6721 agctcgggca ggagcgcaac ctggcccagt ccctggaaaa ctaccaggag atgatccaaa
     6781 tccgccgcct gcggggcggc tggatccggc cggacctgga gtacgacccg gagcggctgg
     6841 aggttgaatt cctgccggac gaggacggcc gggggcgcgc ccgggtctgg aagcttaacg
     6901 gggacggcaa gcgggccggc agcgaggagt ttgcccggga aggcaagaat tggaaatggc
     6961 tgcccgcctc cggctacctc tggtttccgg agcccagcgg cgagctggag gagatcgagc
     7021 ggcggcgcca ggctatggag gccggcctcg ccagcccgaa cccgtttatg cgcttccaga
     7081 cggccgtcaa cctgctggaa cacaagcagt caaagacccg ggcgctccag gtgctccaga
     7141 acctgcttaa ggaacccgat ccgcgcttcc gcctccgggt cgccgaggcc gtgctgggtg
     7201 ccggcgacaa gaacgccgtc ccggttctga tcgacctgct ggcgatcagc gacacttacg
     7261 cgcgcgccaa gttcctgctg gagaagagca ccgggcagaa tttcggcgcc ctgacgggca
     7321 tcacgaccaa ggccgaggtg accaagttcc ttaacgactg gaaggcctgg tggaaccaga
     7381 acaagggctc cttccagctg ccggccgccc cctgatccgg ccccgcggtc cctcttccgg
     7441 agccgctcgg cgcggccgtc gatatttgac gccgcttcac gcttaagagt acaattaaag
     7501 gataatgttt tactttatta ggaggccctt tttatttttt ctgaccctgg cgatcgggct
     7561 ggcgccgctc ctttccggaa cggcccgggc ccaggaggag aaggtcgttc gcgccgtcga
     7621 ggtggccggg aaccaggccg tctcgaccgg ccgggtcctg ctgctgctgc agacccgggc
     7681 gggtgagccc taccgcgagc ggacgctggc cgaggacgtg agccgcctcc tttccaccgg
     7741 cttcttcgcc gaggtggtgc cccggaccga gccgacctcc gacggcggac tgcgggtgat
     7801 cttccaggtg cgcgagaacc cgacgctggc ccgggtcacc ttcagcggca accgcaagct
     7861 gcgcaaggag gtcctgctgg aacggagcgg tctggccgcc ggccagattt acgaggcggc
     7921 ccgagtggcc gaggcggcgg aacggatccg ggatttttac cgggagaaag gactggcctt
     7981 caccacggtc gagccgaagg ccgagctggc cggggagaac cggatggggg tcgtcttcct
     8041 gattcgcgag gcggccgccg gccggatttc ggcgatcggg ttcaccggga accagttcta
     8101 caagagggag aaactgctcc gggtgctcca gctgaaggag cgcagcctgg taaagttcaa
     8161 gctggggacc ttcaacgaga aggccttcca ggacgaccag gcgcggatcc gggcccttta
     8221 ccggcaggcg ggcttcgccg acgtgaaggt ggcgggggcc acccggccgg ccggccggta
     8281 cctggaggtg gatttcagca tcgaggaggg gcggcgctac ctggccggac cggtcaactt
     8341 ttctggtgac ctgctctaca ctccggagaa gctccgggac caggtccgga tgaaggaggg
     8401 ggttccttac agccaggccg gccttgatgc ggaccggtcc agcctgctca acctttaccg
     8461 ggacgacggg tacctcaagg cccgggtgga gccggtggcc gtcttcaaca ccgccagcga
     8521 gcgggtggat atcggctacc aggttgatcc gggcccggtg gtggaggtcg agggggtcga
     8581 ggtttcaggc aatacccgga cccgcgccgg ggtgatccgg cgcgagataa agcttgcccc
     8641 cggcgaccgg tttgacggcc ggaagctccg ccgctcgatc gagaacctgc gcgacctgaa
     8701 ctattttgag gacgtaaaca tccagccgga agagggctcc gaccccggga aggccgtcct
     8761 gaactttgac gtcacggagc gggagcggac cgggaacctg atgttcggag ccggatacag
     8821 cagcgtcgac gggctgatcg gcctgttggc cgtcgaacag accaacttcg actggcacaa
     8881 cccgccctcc ttcatcggcg gcggtcagac cctgcgcctt tccagcgatt tcggacgcca
     8941 gcgccagggt ttttcgctcg gcttcaccaa cccctacata tacgaccggc cggtcctctt
     9001 cggcttcgat ctctacgacc gggaccgcga gtatgacgag tttgacgagc gccggaccgg
     9061 cggcgacctg aggttcgggc gccgggtcaa cgaatactgg agcgtcagcg ccatgccccg
     9121 ggccgagcgg gtggacgttt ccgacatctc tccctttgcc tcgacggagt acctcaagga
     9181 ggagggcgaa aacgacattt acagccttac cctggccgcg gcccgcgata cccgcgattc
     9241 gcggctgcgc accacccgcg gcacccggca ggagctctcc tacaagtacg ccggcggctg
     9301 gctgggcggc gaccgggatt tctggcgtgg cgactacgat tttacctatt accggaaact
     9361 cggaccggcc tggaccttct cctcccagac ccggctcggc atcgccgata ccatgggcag
     9421 cacttcggac gtgcccgtct acgagcggta cttctgcggc ggggcccgga cggtccgcgg
     9481 ctacgaggac cggtcgcttg gaccgctcga cgcctccggc ctggccaagg gcggcaactt
     9541 caccctgatc cagaacctgg aattttcacg gccgatctac caggatatcc tgcacctggt
     9601 ccttttcgcg gactcgggcc aggcctggtg gcgcgccaac gatttcgact tttcggaact
     9661 gaaaagcagc ctgggcgccg gcctccggct caaggtgccc atcttcccgc tgccgatcaa
     9721 gcttgattac gggtatgcgc tggacccgct cccgggggag gactccggcc ggtggcactt
     9781 cctgatcgac tggtggttct gagcggggcg gcaagacagg gatccggctg aagaccgccg
     9841 ggtccgtcag acagaa
//
LOCUS       DAOQYN010000050         9855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_7361_length_9855_cov_4.017959, whole genome shotgun sequence.
ACCESSION   DAOQYN010000050 DAOQYN010000000
VERSION     DAOQYN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7361_length_9855_cov_4.017959"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PKN80_03220"
     CDS             <1..1028
                     /locus_tag="PKN80_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35057.1"
                     /translation="GDLFLQEKNYAAAAGYYLKAEQKTRDSQIRAAANYQLGILARRN
                     RDYAGSGEYFQRVLKIPGDPELVEKAMLELAETAFLQADYQRALDLYREFQRRFPSSR
                     QTEAARLQEAFCLYNLRRYQESQAVFADFIGRFPESPLAGKAYYGSGLVSIARGDKAA
                     AARTWQEFLAGRSTIEDQAPMVLLLCRYLLEADRPAEALPYLKRLSGNASLEAGVRAE
                     ARLISGLAYLKQNDYQPALNEFESGLELPVPGEIREALLKNKADLLLAGGRYAEALPI
                     YQTLKETLPDRRGEVLYGLGVCLQQTGRGREAVPHYLEALINLPADSPLAREIKARLA
                     QIRKANS"
     gene            1061..1930
                     /locus_tag="PKN80_03225"
     CDS             1061..1930
                     /locus_tag="PKN80_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35058.1"
                     /translation="MTKKILPLILILLAAVNVRAQEPIRLPEKLILDENASPFTRFQF
                     PGEVPPPVDPVRLPFGLEPLPKPLSVTLEAGAASRDSFFLRLIQNNERRNFAFGYYQE
                     GEAPFPDPARELRLEFQQLWSLGLVETGLRLAPGFAMRWPAIRRRGTGWEADSSAGGK
                     FLDLSVNTGVRLPTAHRLELEAGGRLLDAVETADLKPWQYLWRGRFSLFGPLGPKWNG
                     QLYLDYQAAPRHSAGTARVERFIPGLRLTWQIDPFSRLGFEFRNFTDQAYEVVPGRYG
                     PGQTVRCYFSRSF"
     gene            2018..3802
                     /gene="lepA"
                     /locus_tag="PKN80_03230"
     CDS             2018..3802
                     /gene="lepA"
                     /locus_tag="PKN80_03230"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNQ35059.1"
                     /translation="MHKNGISHTRNFAIVAHVDHGKSTLADRFLQLCGAVEERNFRNQ
                     LLDSLDLEREHGITIRSHPVTLDYRGYRLNLIDTPGHIDFSYEVEKSLAATNGVILLV
                     DATQGVQAQTVANLQLAQRLDLEIIPAVNKIDLPNSQVEETRRELMEILPVFENEIIL
                     VSAKTGQGVPELLQTVIDQVPAPSSPAGGHLSALVFDARYNPYLGLDLYVVIRSGRVK
                     KGDRLRLLGLGLEAEALAIGIFRPEPSETGLLEPGQVGYVTINIKDFSREFVGDTLAR
                     LEEPSPQPLPGYRRLKQFVFCGLYPGDGEEFPRLKDSIERLHLSDPSFTFSEERSAAL
                     GVGFRAGFLGMLHMSITQERLEREFGVKVVRTTPNTTYRFQLRDGTEREVRSASELPE
                     WSQVREVLEPFLKVLIIAPADYYGEVTGLCQGYRADQKKVEFLGSNLVLFTYEMPLSE
                     MMVDFYDRLQSLTRGYGSLDYEFIDYRPSDLVKLDILINGKALDVLTTVVHREKSRKM
                     AQDLLAKLRKNIPRHLFAVVIQAAVDGKIIGRQEVAPFKKAVTSKCYGGDITRKRKLW
                     EKQKEGKKRMKMVGQVQIPESAFLALYK"
     gene            3815..4396
                     /gene="lepB"
                     /locus_tag="PKN80_03235"
     CDS             3815..4396
                     /gene="lepB"
                     /locus_tag="PKN80_03235"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNQ35060.1"
                     /translation="MADKNNKKTPGWKSELLEWLKSLAVAFILAMFIRAFFFQAFKIP
                     SSSMEPTLKIGDHLIANKLVYRFQSPRRGDVVIFKFPGDGRRDFVKRLIGLPGETVWI
                     SNGQIYINGKETTEPRITNRRYFNFENLPPVQVPGDGLFVLGDNSLNSYDSRYWGFVP
                     RKNLIGKALFIYWPPWRWRMISQLEFSGRPDYC"
     gene            4398..5069
                     /locus_tag="PKN80_03240"
     CDS             4398..5069
                     /locus_tag="PKN80_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNQ35061.1"
                     /translation="MKTRPNHNQKSPEPESGIPARETPVNPEAASEKEMDAPAPETEA
                     DPGVLLKEAADRAELLQEKMLRLAAEFDNFRKRTSLEKERLTTDAAAGVLAKLLPVLD
                     SLDQFQLHAGSGEAEKEPNEGLAVLTRQLAQVFQACGLERLELKGRMFDPHYAEAVEI
                     YHDPEAADGVVLEVLRAGYRYRERILRPALVRINRRPDAEADSTQKPGPESEAAADDS
                     TIEEA"
     gene            5069..6106
                     /locus_tag="PKN80_03245"
     CDS             5069..6106
                     /locus_tag="PKN80_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35062.1"
                     /translation="MSREIRVGIAGLGRTGWFNHAKTLETMAGSYRITAVQDPNADRQ
                     KEAAGAFGCRTYSDFDQLLADPEVELVVVATPTMLHTEQTIAALKAGKMVVCEKPLAT
                     TAAEVDRMIAAARTTGRLFTIFQVRRYAPDFLKIREILSSGCLGRILTIKIYAHNFSR
                     RWDWQTLRKHGGGILFNHGSHYLDQVLLLMPPGEIELFCHLERVLALGDAEDHAKLVL
                     RAGNGLVADLELTSSSAYSQDFWFIMGNQGGLRGSASRLQWKYFDPARLPKKELDEAP
                     VADRTYNRDQIPWEPEQEWTAPTGQPSPSSLFYRDLYQTIVNGAPLAVTPESVRRQVA
                     LLEKCHQMAGI"
     gene            6121..6981
                     /gene="ispE"
                     /locus_tag="PKN80_03250"
     CDS             6121..6981
                     /gene="ispE"
                     /locus_tag="PKN80_03250"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNQ35063.1"
                     /translation="MSAEIVQAPAKINLFLEVFPRPPEARLHPVDTVLEKVDLCDLVR
                     LEVTPGRIELECNLPELANQENLAWRAAVLLKERFSVRAGVRIGLEKRIPLQAGLGGG
                     SSDAAAVLLTLNRLWALQLPRSELDGLALELGSDVPAFLEPGRCRATGYGQQVAPLPF
                     KSRWNYLLFLTGIAISTGEAYREIDNLTYLPASSKIILRGVELDDFSGVSKGLFNRFE
                     EVALSTRDEVKRFKEEIDRFYPHNLLCGSGGAFFALVKEAEAGETGNIQRLSQRLRCR
                     IVSVTGIRGS"
     gene            6983..7366
                     /locus_tag="PKN80_03255"
     CDS             6983..7366
                     /locus_tag="PKN80_03255"
                     /inference="COORDINATES: protein motif:HMM:NF015959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HNQ35064.1"
                     /translation="MEITETRVSPVEKPSGRLKGFASVTFDGVFVVRGIRIIEGDNGL
                     FIAMPNRKTEFRCPGCGGWNVFHAKFCNRCGKEIAMSQPHDKNEDDPKNYHDIVHPIN
                     NEFREIIQSHILNEYQSETQGRKTL"
     gene            7393..8241
                     /locus_tag="PKN80_03260"
     CDS             7393..8241
                     /locus_tag="PKN80_03260"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006789993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNQ35065.1"
                     /translation="MDFQTLLAQLLDFWRQQGCLVYHPTGLEVGAGTMNPATFFKVLG
                     PEPWRTAYLEPSRRPADGRYGENPTRLYQHYQLQVILKPSPAQTKRLYLESLQAIGIS
                     WRKHDIRFIDDNWESPTLGAWGVGWEVWLDGLEITQFTYLQQAGGLELKPISVELTYG
                     LERLACFLQKKRVIYDLDWSADWNYGQLRHRAEAEYSRYSFEAADIDRHFELFNHYEQ
                     EASQLIKRQLVWPAYDYVLKCSHTFNTLESRGAISLSERQRFIGRVRKLASACARLYL
                     GDIINN"
     gene            8245..8326
                     /locus_tag="PKN80_03265"
     tRNA            8245..8326
                     /locus_tag="PKN80_03265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8279..8281,aa:Leu,seq:gag)
     gene            8337..8410
                     /locus_tag="PKN80_03270"
     tRNA            8337..8410
                     /locus_tag="PKN80_03270"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8371..8373,aa:Val,seq:gac)
     gene            8535..9698
                     /locus_tag="PKN80_03275"
     CDS             8535..9698
                     /locus_tag="PKN80_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35066.1"
                     /translation="MEKVRIGVIGCGVMGRSHLTKLKEVPLAELTAVADAFPESARKA
                     GAEFGVPAYGDYRELISSGRCDAVLIATPHYFHPEIGLAAFQAGLHVLCEKPIAVTVR
                     EADALVAAAEKSGRVFAVNFQMRTVPEIRFARRLLAEGRLGEIRRTMVVENAYRTQAY
                     YDSAGWRGTWIGEGGGVLINQAPHGIDLFLLLGGLPSRVLARTRTSRHDIEVEDEACA
                     LLEYPNGAWGYYYASTSELPIGHRIEVVGDRDRLVYSIRDDQYRELKFQSFNPPLSKH
                     ISQSPEMWTGVELKDEPIELPAVETGHGEIIRNFCAAILHGEALLSPGREGLWSVEFI
                     NSLILSGHRGREVSWPTPRDEYDQLLAELKKNSRLRQVAPKTASKGAPGVDRI"
BASE COUNT         1988 a         2998 c         2986 g         1883 t
ORIGIN      
        1 tgggtgatct tttcctgcag gaaaaaaatt acgcggcagc ggccgggtac tacctgaaag
       61 ccgaacagaa gacccgggat tcccagatcc gggcggccgc caattaccag ctggggatcc
      121 tggcccgccg gaatcgggac tatgccggtt ccggagagta tttccagcgg gtactgaaga
      181 ttccgggcga cccggaactg gttgagaagg cgatgctgga gctggccgag acggcttttc
      241 tgcaggccga ttaccagcgg gcgctggatc tttaccgcga attccagcgc cgttttccct
      301 ccagccgtca aacggaagcc gcccggcttc aggaggcctt ctgcctttat aatctgcgcc
      361 gttaccagga aagccaggcg gtcttcgccg attttatcgg gcgttttccg gagagtcccc
      421 tggcgggcaa ggcctattac gggtccgggc tggtctccat tgcccggggt gataaggccg
      481 ccgccgccag gacctggcag gaattcctgg ccggtcgcag caccatcgaa gaccaggcgc
      541 cgatggtcct gcttctttgc cgctacctgc tggaagcgga ccggccggcc gaggcgcttc
      601 cctatcttaa acggctcagc ggcaacgctt ccctggaggc cggggtgcgg gcggaggcac
      661 gtctcatcag cggattggcc tatctcaaac agaatgatta tcagccggcg ctcaacgagt
      721 tcgaatccgg actggaactt ccggttccgg gggagatccg cgaagcgctg ctcaagaaca
      781 aggccgatct gcttctggcc ggcggaagat atgcggaggc cctgccgatc taccagacac
      841 tgaaggagac cctgccggac cgtcggggtg aggtgcttta cgggctgggg gtctgcttgc
      901 agcagaccgg tcgcggccgg gaggcggttc cccattacct ggaggccctg atcaatcttc
      961 cggccgattc gcccctggcc agggaaatca aggcccgcct ggcccagatt cgcaaagcca
     1021 attcctgact ccggccggcg gccacaggga tcaaaccaag gtgaccaaga aaattcttcc
     1081 gctgatctta atcctgctgg cggctgtaaa tgtccgggcc caggaaccga tccggcttcc
     1141 ggagaaactt attctggacg aaaacgcctc tccgttcacc cgtttccaat tcccggggga
     1201 agtcccgccc ccggttgatc cggtccggct tccctttggc ctggagccgt tgcccaagcc
     1261 gctctcggtt acgctggaag caggagcggc ctcgcgggac tcctttttcc tgcgcctgat
     1321 tcaaaacaat gaacgccgca attttgcctt cggctattac caggaggggg aagcgccttt
     1381 tccggacccg gcccgggaat tgcgccttga gttccagcaa ctttggtcgc tgggcctggt
     1441 ggagaccggc ctcaggctgg cgccggggtt cgccatgcgc tggccggcga ttcggagacg
     1501 cggaaccggc tgggaggccg attcttccgc gggcggaaaa tttctggacc tgtcagttaa
     1561 caccggagtt cggctgccga cggcgcatcg gctggaactg gaggccggcg gccggctgct
     1621 ggatgcggtc gaaaccgccg acctcaaacc ctggcaatac ctctggcgcg gccggttttc
     1681 cctgtttggt ccgctggggc cgaagtggaa cggccagctt tacctcgatt accaggcggc
     1741 gccgaggcac agcgccggaa cggcccgggt ggaacgtttt atacccggcc tccggctcac
     1801 ctggcagatt gatcctttca gccgtctggg tttcgaattc cgcaatttca ccgatcaagc
     1861 ctacgaggtg gtgccgggtc gttacgggcc cggacagacc gtccgatgct acttcagccg
     1921 ttccttttaa atctctccgt gttataatat tccaatctga ttcgggttcg ggaaacgccg
     1981 ggacccgtcc cagcaaactt gccacgccgg tttaagcatg cacaaaaacg gaatttccca
     2041 cacaagaaat tttgccatcg tggcccacgt cgaccacggt aaaagcacgc tggccgatcg
     2101 tttcctccag ctttgcggag cggttgagga acgcaacttc cggaaccagc tgctcgacag
     2161 ccttgacctg gagcgggaac acggcatcac cataagatct cacccggtta cgcttgacta
     2221 ccggggttac cgactcaacc tgatcgacac tcccggccac atagattttt cctacgaagt
     2281 cgaaaaatcg ctggcggcca ccaacggggt aatcctgctg gttgacgcca cccagggggt
     2341 ccaggcccag acggtggcca accttcagct ggcccagcgg ctggacctgg agatcattcc
     2401 ggccgtcaac aagatcgacc tgcccaacag ccaggtagaa gagacccgtc gggaattgat
     2461 ggaaatcctg ccggtcttcg agaacgagat catcctggtc agcgccaaga ccggccaggg
     2521 cgtcccggaa ctcctccaga cggtcatcga ccaggtcccg gccccgtcat ccccggccgg
     2581 cggtcatctt tcggcgctgg ttttcgatgc ccgttataac ccttacctgg gtcttgacct
     2641 ttacgtggta attcggtccg gccgggtcaa aaaaggagac cggctccgcc tgctcggtct
     2701 cggcctggaa gcggaggctc tggccatcgg gatcttccgg cccgaaccca gtgaaaccgg
     2761 tttgctggaa ccgggccagg tcgggtacgt gaccatcaac atcaaggatt tcagccggga
     2821 attcgtgggt gacacgctgg cccggttgga agagccctct ccgcagcctc ttcccggcta
     2881 tcgccgcctc aaacagttcg tcttttgcgg cctttatccc ggcgacgggg aggaatttcc
     2941 gcgtttgaag gacagcatcg aaaggctgca cctgagcgac ccgtccttca ccttcagcga
     3001 ggaacgctca gccgctttgg gggttggttt ccgggccggg ttcctcggaa tgcttcacat
     3061 gagcatcacc caggaacggc ttgaaaggga attcggggtg aaggtggtgc gaaccacgcc
     3121 caataccacc taccgcttcc agctgcgcga cgggaccgaa cgcgaagtcc ggagcgcctc
     3181 cgaactcccg gaatggagcc aggtccgcga ggtcctggag ccctttctca aggtgctcat
     3241 aatcgcgccg gccgattatt acggtgaggt aaccggtctc tgccagggtt accgggccga
     3301 ccagaagaag gttgagttcc tgggttcaaa cctggttctc ttcacctacg aaatgcccct
     3361 ttcggagatg atggttgatt tctacgaccg ccttcaatct cttacgcgcg gctacggttc
     3421 gcttgattac gagtttattg actatcggcc gtccgattta gtaaaattag atattttaat
     3481 aaacggcaag gccctggatg tcctgaccac cgtggtgcac cgggagaagt cccggaagat
     3541 ggcccaggac ctgttggcca aactccggaa gaacattccc cgtcacctct tcgcggtggt
     3601 catccaggcg gctgtcgacg gcaagatcat tggccgccag gaagtggccc ccttcaaaaa
     3661 ggccgtcacc agcaagtgct acggcggtga tatcacccgc aagcgcaagc tctgggaaaa
     3721 gcagaaggaa ggcaagaagc gcatgaagat ggtcggccag gtgcagatcc cggaaagcgc
     3781 cttcctggcc ctttacaagt gaggcggaaa aagcatggcg gataagaaca acaagaagac
     3841 cccgggctgg aaatcggaac tgctggagtg gttgaaatca ctggcggtgg ccttcatcct
     3901 ggccatgttc atccgggcct tcttctttca ggcctttaaa attcccagca gttccatgga
     3961 accgaccctg aagatcggcg accacctgat cgccaataaa ctggtctacc ggtttcaatc
     4021 gccccggagg ggtgacgtgg tcatcttcaa gttccccggg gacggccgcc gcgatttcgt
     4081 caagaggctg atcggcctgc ccggtgaaac ggtctggatc tccaacggcc agatatacat
     4141 caacggcaag gaaacgaccg agccgcggat taccaaccgc cggtatttca acttcgaaaa
     4201 cctgcccccg gtccaggtgc ccggcgacgg ccttttcgtg ctcggagaca acagcctcaa
     4261 cagttacgac agccgttact ggggattcgt gccccgtaag aatctgatcg ggaaggccct
     4321 cttcatctac tggccgccct ggcgctggag gatgatttcg cagctggaat tttcgggccg
     4381 gccggattac tgctgacatg aagacccgcc ctaatcacaa ccagaaaagt ccggaaccgg
     4441 agtccggaat cccggcccgg gagacccccg tcaatccgga agccgcctcc gaaaaagaaa
     4501 tggatgcgcc ggcgccggaa acagaggccg atcccggggt gttgctgaaa gaagcggccg
     4561 atcgggccga gctgcttcag gaaaagatgc tccgcctggc ggccgaattc gacaatttcc
     4621 ggaagcggac cagcctggag aaggaacgcc tgacgaccga cgccgcggcc ggggtgcttg
     4681 cgaaactcct gccggtgctg gacagccttg atcagtttca gctccacgcc gggtccggcg
     4741 aggctgagaa ggaaccgaac gagggtttgg ccgtcctgac ccgccagctg gcccaggttt
     4801 tccaggcgtg cggattggaa cggctggaac ttaagggccg gatgttcgat ccccattacg
     4861 ccgaggcggt cgaaatatac catgatccgg aagccgccga cggcgtggtg ctggaagtcc
     4921 ttcgggccgg ctaccgctac cgggagcgga ttctcaggcc ggcgctggtc cggattaacc
     4981 gccggccgga cgcggaagcc gattctactc agaaaccggg gccggaatcc gaggccgcgg
     5041 cggacgactc aaccatcgag gaggcataat gagcagggaa atccgggtgg gtattgccgg
     5101 attgggacgg accggctggt ttaatcacgc caaaacgctc gagacgatgg cggggagcta
     5161 ccggataacc gccgttcagg atcccaacgc cgaccgccag aaagaggccg ccggggcctt
     5221 cggctgtcgg acttactctg attttgacca gctgctggcc gatcccgagg tggagctggt
     5281 ggtggtggcc accccgacca tgctgcatac cgagcagacc attgcagcct tgaaggccgg
     5341 caagatggtt gtctgcgaaa aacccctggc caccacggcc gccgaggtgg accggatgat
     5401 tgccgccgct cgcacgaccg gacgcctgtt caccatcttc caggtccgcc gctatgcgcc
     5461 ggattttctc aagatccggg aaattctttc cagcggctgt ctggggcgga tcctgaccat
     5521 caagatctac gcccacaact tcagccggcg ctgggactgg cagacactcc ggaagcacgg
     5581 cggcgggatt ctttttaacc acggatcgca ctatcttgac caggttctgc ttttgatgcc
     5641 gccgggagaa atagaattgt tctgccacct ggaacgggtg ctggccctgg gtgatgccga
     5701 ggaccacgcc aagcttgttt tgcgggcggg aaacggcctg gtggccgacc tcgagcttac
     5761 cagcagcagc gcctacagcc aggatttctg gttcataatg ggtaaccagg gcgggctgcg
     5821 cggcagcgcc tcccgccttc agtggaaata tttcgatccg gcccggctgc cgaaaaagga
     5881 actggacgag gcgccggtgg ccgaccgcac ttacaaccgg gaccagattc cctgggagcc
     5941 ggagcaggaa tggaccgccc cgaccggcca accttcacca tcatccttgt tctaccggga
     6001 tctttaccag accatcgtca acggggcgcc gctggcggtt accccggaaa gcgtgcggcg
     6061 ccaggtggcc ctgttggaaa agtgccacca aatggccgga atctgaaccg gccgccggtt
     6121 atgagcgcgg agatcgtgca ggccccggcg aaaattaacc tgtttctcga ggtttttcca
     6181 cggccgccgg aagcccgcct gcacccggtg gacacggtgc tggaaaaggt cgatctctgc
     6241 gatctggtcc gcctggaggt caccccgggg cgaatcgaac tggagtgcaa tctgcccgag
     6301 ctggcgaatc aggaaaacct ggcctggcgg gcggccgttc ttctgaaaga gcgtttctcg
     6361 gtccgggccg gggtccgcat cggtcttgag aagcgcattc cccttcaggc cggcctgggc
     6421 gggggcagca gtgacgcggc cgccgtcctg ctgaccttga accgtctctg ggccctgcaa
     6481 cttccccggt ccgagctgga cgggctggcc ctggaactcg gctcggacgt gccggccttc
     6541 cttgaaccag gacgatgccg ggccaccggt tacggccagc aggtcgcccc cctacccttc
     6601 aaaagccgct ggaattacct cctctttctg accgggatag caatttcaac cggggaggcc
     6661 taccgcgaaa ttgataactt gacatactta ccggcttcca gtaaaattat tttgaggggg
     6721 gtggaattag atgatttttc aggtgtttcc aaaggactct tcaatcggtt tgaagaggtt
     6781 gccctctcca cccgggacga ggtgaagcga ttcaaagagg agattgaccg attttatcct
     6841 cacaacctgc tgtgcggcag cggtggggct tttttcgcct tggtcaagga ggcagaagcg
     6901 ggagaaaccg ggaacattca gcgattgagt cagcgcctgc gttgcagaat cgtatcggtt
     6961 accggcatca gggggagttg aaatggaaat caccgaaacc agggtttctc cggtagaaaa
     7021 gccgtccggt cgtctcaagg gttttgcctc ggtcaccttc gacggggtct tcgtcgtgag
     7081 gggaatcaga atcatcgaag gtgataacgg actcttcatc gccatgccca accgcaagac
     7141 ggaattccgc tgcccgggct gcggcggctg gaatgttttt cacgccaagt tctgcaaccg
     7201 ctgcggcaag gaaatcgcca tgtcgcagcc gcacgataag aatgaggacg atccgaagaa
     7261 ctaccacgat atcgtccacc cgatcaacaa cgaattccgg gagattatcc agagtcacat
     7321 cctgaacgaa taccaaagcg aaacccaggg caggaagacg ctctgaaaga ttcgcgaacg
     7381 ctggaactca acatggattt tcagacgctt ctggcccaac tgctggactt ctggcgccag
     7441 cagggatgcc tcgtttacca tccaaccggc ctcgaggtgg gcgccggaac gatgaacccg
     7501 gccaccttct tcaaggtgct cggccccgaa ccctggcgga ccgcctatct cgaaccttcc
     7561 cgccggccgg ccgacggccg ttacggagag aatcccaccc gtctctacca gcactaccag
     7621 ctccaggtga tcctgaagcc atcgccggcc cagaccaaac gcctttacct ggaaagcctt
     7681 caggctatcg gcatttcctg gcgcaagcac gacatccggt ttatcgacga taactgggag
     7741 tcgcccaccc tgggcgcctg gggcgtgggc tgggaggtct ggctggacgg cctggaaata
     7801 acccagttta cctaccttca acaggccggc ggtcttgaat tgaagccgat ttcagtggaa
     7861 ctgacctacg ggctggaacg gctggcctgc ttcctccaga aaaaacgggt gatctacgac
     7921 ctggattggt cggccgactg gaattacgga cagctgcgcc accgggccga ggccgaatat
     7981 tcccgctaca gctttgaggc cgccgatatt gaccgtcact tcgaactttt caaccattac
     8041 gaacaggagg caagtcagct tatcaagcgg cagctggtct ggccggccta cgattacgtg
     8101 ctgaagtgtt cccatacctt caacaccctg gaaagccggg gggcgatcag tctgagcgag
     8161 cgccagcgtt tcatcgggcg cgtgcgcaaa ctggcctcgg cttgcgcccg gctctacctt
     8221 ggagatataa taaataatta gcatgccggg atggtggaat tggcagacac gtacgtttga
     8281 ggggcgtatg gggaaaccct tgtgggttca aatcccactc ccggcactgg attgacgggc
     8341 ggttagctca gctggttaga gcgccacgtt gacatcgtgg aggtcacagg ttcaaatcct
     8401 gtatcgccca ccgttgcttt cagaaccaag ctaccgaacc cccttttctt cctgcagctg
     8461 ccgcggtgcc cggggcagca tttccgcccc cacccttcca accgccggcg ctgaaacccg
     8521 tggagattag actaatggaa aaagtgcgga tcggcgtgat cggctgcggt gtcatgggcc
     8581 gaagccacct gaccaagttg aaggaagtgc cgctggcgga attgaccgcg gttgcggatg
     8641 ccttcccgga gagcgcccgg aaggccggtg cggaattcgg ggtacccgcc tacggcgact
     8701 atcgggagct tatttccagc ggccgctgcg acgcggtcct gattgccacg ccccattact
     8761 ttcacccgga aatcggactc gccgcctttc aggccggcct tcacgttctc tgcgagaaac
     8821 cgatcgcggt tacggtgcgc gaggccgacg cgctggttgc cgccgccgaa aagagcggcc
     8881 gggtctttgc ggtgaacttc cagatgcgca cggttcccga gatccgtttc gcccgccgcc
     8941 tcctggccga ggggcgtctg ggcgagatcc ggcggacgat ggtggtggag aatgcctacc
     9001 ggacccaggc ctattacgac agcgccggct ggcgcggcac ctggatcggc gaaggtggag
     9061 gcgtgcttat caaccaggcc ccgcacggga tagacctttt cctcctgctg ggtggcctgc
     9121 caagccgggt gctggcccgg acccggacca gtcgtcacga catcgaggtc gaggacgagg
     9181 cctgcgccct gctggagtac ccgaacggcg cctggggcta ttattacgcc tccacctcgg
     9241 aactgcccat cggccaccgg atcgaggtgg tcggcgatcg ggaccgcctg gtttacagca
     9301 ttcgcgacga ccagtaccgg gaattgaaat tccagtcctt taacccgccc ctctcaaaac
     9361 atatcagcca atccccggag atgtggaccg gggttgaatt gaaggatgag ccgatcgaac
     9421 ttccagccgt ggaaaccggt cacggcgaga tcatccgcaa cttctgcgcc gccatcctcc
     9481 atggggaagc actgctttcc ccgggccggg agggactctg gagcgtggag tttatcaaca
     9541 gcctgatcct ctccggccac cggggccgcg aggtttcctg gccgaccccg cgggatgaat
     9601 atgaccagct cctggctgaa ttgaagaaaa actccaggct gcgccaggtg gctccgaaaa
     9661 ccgccagcaa gggggcgccg ggcgtggacc ggatttaacg ccggcttgtt ttcaaaacaa
     9721 atggcgatga aacaggtccg gatcggggtt atcggctgcg gcggcatggg ccaggggcat
     9781 atcgccctca tcaacgagct gcccggggcc cggctggccg cggtcgccag ccagacaccg
     9841 gagaatgccc gctcg
//
LOCUS       DAOQYN010000051         9652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_7550_length_9652_cov_5.499323, whole genome shotgun sequence.
ACCESSION   DAOQYN010000051 DAOQYN010000000
VERSION     DAOQYN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7550_length_9652_cov_5.499323"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..3756)
                     /locus_tag="PKN80_03280"
     CDS             complement(202..3756)
                     /locus_tag="PKN80_03280"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNQ35067.1"
                     /translation="MSWQRWLMLPILLGFLSIGPGPAPAADKPAGFFVEAEDFDGLEE
                     FEIWQTVAGRWYAKEHLYGETSALGGAYAVANEFSMGAVMEKKLDRVLPPGRYRVWLR
                     MIAWMDSDDAIEVELNGAKLVYAWDINTRGRGYRWMPGEITTTQAGNLMRVKALKGNQ
                     QNFGETPRPPCKFLAIDTFYIGPADEKVRAVVSSRSSDDLVFGDVADPAAKPAPAAAA
                     VRPETPPAGNLVANGSFEAGIGHGWTPFGFKAGILDAAFLDTEAVHGHYSLRSRHPVT
                     GAAIPQLMSRLYRIKADSVYTLSAWFRGAKGFSLGLLTEDGKPVLSQPARGTGKWERV
                     FVSGRVGPGDYYILISGEDFSVDAVQLEAAETASDFQPAVPVEVGFSQDMVGRVYFAG
                     EPARARLLCALDRSSRLRSVTVDLTVHDIWDRVVRKDKLTLPVRPGETAERWVDLNPG
                     RTGMFRAEAVVAGLPQAREETVFSVLPRPRTMGIDHDSWLGVMPEYNEYILASLQRAG
                     YKWVQNIRVGNPTRWPYAEPEKGRIIWYDNLIGQPARYGLEEIFLLHMNYPSLPAWVK
                     PSVANKLVPEDITLWQDFVRRTVEHYKDRVKYWQFSDDIHHYFSADEHYQCLKATYEA
                     AKQADPGCTFIGWRFYLREVPGWEADLARTEPYSDIIYAADRVMREKYRKPAHSYLFF
                     RGTTMYDYSTLGDPERRQALGELRRQVCLDVVRNFCEHAANVGYVDALFFYQAFLGGT
                     QPWQTGMSKRAFEHDGALHLGAITPRIVEHFLYKADCLGRVNTGPEIRAYLFRKPGAA
                     VAALWSNGGKEVRVGLDKKLKNLQVYDGMGNLLQPPVESGRLVITLLDTPVFLVAEGT
                     PAEELGRLASGASLTLPIPIARRFTVREGRLAMELLVGNNTRQPISARVSLPEPMLFP
                     LAGGAETEVTLAAIPPAGEGKAVFPLKYGLDRNGEFKRLMLDIQAGDYTFRETWLLWL
                     LNSRPAAAGIKLTGDPAAWSGVLPVKLVTTDRSGATRRLTQVRSGAITGEEALTQGMA
                     PILQGKSDLSGRVYSQWDAQNLYFAAEVLDNSVEKGDRVDIYLSNGGTPPSGPAGRIF
                     DLSYRVDESGARAILKSGEESREVPAAFRKTAGGYFLELSVPWTALGLKPEAGRLLGF
                     DASLTDSDQGVFHAQLVWSGAAYSAGDPAGFGVLAIGK"
     gene            complement(4158..8501)
                     /locus_tag="PKN80_03285"
     CDS             complement(4158..8501)
                     /locus_tag="PKN80_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35068.1"
                     /translation="MNATRRFLRPSLLMLLSLLGLFSCNTAWSAPPQVLFQAGFEKRA
                     DGFGRDRVYTNFFTSKGAPLTIFEYEKGVVGQGARFTWKNGYPGVAYPVEKNYDSQSG
                     TISLWVRITRPFFVNSYYPLIWCVGAGERVEAAAHDLVLVGLNDPRTEAVADGQWHQL
                     VYCWDGAARKRWQYLDGTLLAEGDYLKPYRPKELIFGFRLPGALDEVVILDRPLSAAE
                     VKDAYGVYRQGKAVFSLAEPGRRDYPVLPLEIGQLPSVRPELPAAAGWLVLQPAAENG
                     KRFRYDLDGLWRFQPAVPEVRDKELVNPGLFGPEWLYVKAPGSWRAANAVLDQTGKPV
                     ASWQGKTFNKLPAAWLERDINLPPRLKNYQFYLTFPRLGGPAEVYLNGKHLGSVARSG
                     EKRFLITRQLLWDRPNRITLLNGLPFAPGMTPTGFEMTPCIEARETKDFYLGDPLICP
                     SFRKQALNLQLPVENTGGSALDLVVAGRAVDYRDPKQAVELGSVSLKVPKDFQGVRTV
                     TLPAAVLKTWSPETPNLYRLVLEIGKGGQPVDLSFPESFGYRELWVADGNIYLNGVKL
                     SIRGKSHNYLAGYGFTRDEIERLQAVGQNADRTLSPVYPAINSLQTADETGWMVFYMA
                     PGVNPDDLETSREEWETLLKQVGNHPSIIAWQWWGNGYVNGKHGHPTQVGGIVPEEIQ
                     RDNWAYQQNRALDGIDPTGRLTFYYRLGAGGDFRGIMTTLGFGTPIQESEEWLTVWAK
                     NRPDPLVPTEMQVVPIHHEAILWQKGSKEIILLEQHARYFGDTGYSKVTEPMIDAHLD
                     PAAKISAWINSDAEIRNDVYDLAYTRVLRSWRTYGISGYLFHGTFKLGEIYYGSKLGR
                     FGETLQRVNLPFLFYLGGPAADFVSKDHNYFAGETVEKSAIIVNDNFHPVSGRISWQA
                     AGGGRTLASGSADVTVPAGGTHFQPLRFETAGLAGKTGVEISAAFKSDDGKLDVSDRF
                     ALTVFPRPAAAAAAGGRVALIDSRGETAAALEKLGVPYQLFSEKTAEAGGRSSLAGYP
                     LLVIGRNSYPEAARLFKESLPIGQAVAEGLNVVILEQSNRQVAGLSVESFKDRRAFIR
                     NGASALLADLVNEDFYDWRGASRSLPAYPPLHESTEPDNSYKYGQNNSFGQRRFWHWS
                     NKGIVATFTFEKPQLGNFRVLMDEGFDLLYTPLVEFYQGKGRILFCQLELVDRFGVDP
                     VATHLLERIIRDYRQPSGRAWAALAHPGGERAKIRLDELAVDYQPGWNGRVAYLDLSE
                     APGQAAALGPFLQGGGTLVTTFRDAAAVQSLPVKVTLEKKKFWKPEVPADPLFAGVGP
                     SDFSFRRPLELLQVTAVDGQPVSNGVAGVIPVGRGRIVYLQVEPGQFENAWQRGKVLR
                     IYNTLLTNLGVRSRVVPDIGAIGGYGMVEEWLTGYEPSVPDIANRPMVKESNLYRKPA
                     LDFDPNRHMVW"
     gene            8721..9503
                     /locus_tag="PKN80_03290"
     CDS             8721..9503
                     /locus_tag="PKN80_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35069.1"
                     /translation="MRNASQAIWLWSHVENLDWSKWGLDRTSRITPAAAAEFLGIENL
                     LLVTYADQPAPPFREYAAGLAGLKQVVWSALGAANCRRGSDLSAVLELVPEMKNLTGV
                     ILDDFFNRRDRDGNPGTYTRAELMEMRRRLAATGRPLELWGVLYNHQLELPVAEKISA
                     WDAVTFWHWTAEELERLETTLPRFEKLAAGRRKLLGLYMYDFGNRRPLPLSLMERQCR
                     IGRDLLAAGRIDGLIFLSSILGDQNLEAVEFTRGFVKENFRP"
BASE COUNT         1728 a         3221 c         3065 g         1638 t
ORIGIN      
        1 ccgtgctcgc tcagcaattc cgccagccac ccgatccggt cgtcgccccc ctcggactcc
       61 ggccggtaaa tatcctcaac gtcaaaggtc aacaggatgt ccgtcattcg cttctccccg
      121 accggccgcc ggtccgaaag gaaaaccgcg ccggagcggc cggcggacga tacccggccc
      181 gccggccgtt accgttgacg tttacttgcc gatggccagc accccgaagc cggccgggtc
      241 accggccgaa taggcggcgc cggaccagac cagctgggcg tgaaagacac cctgatcgct
      301 gtcggtcagg gaggcgtcaa atccgagcag ccggccggcc tccggtttca gacccagggc
      361 cgtccaggga acggaaagct ccaggaagta accgccggcc gttttccgga aggccgccgg
      421 cacctcccgg ctctcttcac cggacttcag gatggcccgg gcgcccgact catccacccg
      481 gtaggaaagg tcgaaaatcc ggccggccgg acccgaaggg ggcgtcccgc cgttggagag
      541 atagatgtcc acccggtcgc ccttctcgac gctgttgtcc agaacttcgg cggcgaagta
      601 aagattctgc gcatcccact ggctgtaaac ccggccggag agatcggact tgccctgcag
      661 tatgggcgcc atgccctggg tcagggcttc ctcgccggtg atggcgccgc tgcgcacctg
      721 ggtaaggcgg cgggtggcgc cgctgcggtc ggtggtaacc agtttcaccg gcagtacccc
      781 ggaccaggcg gccgggtccc cggtcagctt gataccggcg gccgccggac gcgaattgag
      841 cagccagagg agccaggtct cccggaaggt gtaatccccg gcctggatgt ccagcatcag
      901 gcgtttgaac tcgccattcc ggtccaggcc gtacttcaag ggaaagaccg ccttcccctc
      961 gccggccggc ggaatcgcgg ccagtgtcac ctcggtctcg gcgccgccgg ccaacggaaa
     1021 gagcatcggc tccggcaggc tgacccgggc cgaaatcggc tggcgggtat tgttgcctac
     1081 cagcagctcc atggccagcc gcccctcccg aacggtgaaa cgccgggcga tcggaatcgg
     1141 cagggtcagc gacgccccgg aggcaaggcg gcccagctcc tcggccggcg tgccctcggc
     1201 caccaggaag accggcgtgt ccagcagggt gatcaccagc cggccggatt cgaccggcgg
     1261 ctgaagaagg ttgcccatcc cgtcgtaaac ctggagattc ttaagctttt tatccaggcc
     1321 gacccgcacc tccttgccgc cgttggacca gagcgcggcc accgccgcgc ccggcttccg
     1381 gaaaaggtag gcccggatct ccggaccggt gttgacccgg cccaggcaat cggccttgta
     1441 gagaaaatgc tcgacgatcc ggggggtgat ggcgcccagg tgcagggcgc cgtcatgttc
     1501 gaaggctctc ttgctcatcc cggtctgcca gggctgggtg ccgcccagga aggcctggta
     1561 gaagaagagg gcgtccacgt acccgacatt cgcggcatgt tcgcagaagt tccgcaccac
     1621 gtccaggcag acctgccgcc ggagttcgcc cagggcctgc cggcgctccg gatcgccgag
     1681 ggtcgagtag tcgtacatgg tcgtgccgcg gaagaagagg tagctgtggg ccggtttccg
     1741 gtacttctcc cgcatgaccc ggtcggcggc gtagatgatg tccgagtagg gctcggtgcg
     1801 ggcgaggtcc gcctcccagc ccggcacctc gcgcaggtag aaacgccagc cgatgaaggt
     1861 acagcccgga tcggcctgct tggccgcctc gtaggtggct ttcaggcact ggtaatgctc
     1921 gtcggcgctg aagtagtggt ggatatcgtc ggagaattgc cagtatttga cccgatcctt
     1981 gtagtgttcg acggtgcgcc ggacaaagtc ctgccagagc gtgatgtcct ccggcaccag
     2041 cttgttggcg accgacggct tcacccaggc cggcagggag ggatagttca tgtgcagcag
     2101 gaaaatctcc tccaggccgt atctggccgg ctggccgatc aggttgtcgt accagatgat
     2161 gcgccccttt tccggctcgg cgtaaggcca gcgggtcgga ttgcccacgc ggatattctg
     2221 gacccacttg tagccggccc gctggagaga ggccaggata tactcgttgt actcgggcat
     2281 cacgcccagc cagctgtcat gatcgatgcc catcgtccgc ggccggggca ggaccgaaaa
     2341 gacggtctcc tcccttgcct gcggaaggcc ggcaactacc gcctcggccc ggaacatccc
     2401 ggtccggccg ggattcaggt caacccagcg ctcggccgtc tcgcccggcc gtaccggcag
     2461 ggtaagttta tccttccgga ccacccggtc ccagatgtcg tgaacggtca ggtcgaccgt
     2521 cacgctgcgc aggcggctgg acctgtccag ggcgcagagc agccgggccc gggccggttc
     2581 cccggcaaag taaacccggc cgaccatatc ctggctgaag cccacctcaa ccggaacggc
     2641 cggctggaaa tcggaggccg tctccgccgc ctccagctgg accgcgtcca ccgaaaaatc
     2701 ttcgccgctg atcaggatat agtaatcgcc cggcccgacc cggccggata cgaagacccg
     2761 ctcccacttt ccggtgccgc gggccggctg gctgagcacc ggtttgccgt cttcggtcag
     2821 gagacccagg gagaatccct tggcaccccg gaaccaggcc gagagggtgt aaaccgagtc
     2881 tgccttgatc cggtagaggc ggctcattaa ctgggggatg gcggcgccgg tgaccgggtg
     2941 acgggaacgc agggaataat ggccatggac cgcctcggta tccaggaagg cggcgtccag
     3001 gatgccggcc ttgaatccga atggcgtcca gccgtggccg atgccggcct cgaagctgcc
     3061 gttggcgacc aggttgccgg ccggcggcgt ttcgggccgg accgcggcgg ccgccggcgc
     3121 cggcttcgcg gccggatccg caacgtcgcc gaagaccagg tcgtcactgc tgcggctcga
     3181 gaccaccgcc cggaccttct cgtcggccgg gccgatgtag aaggtatcga tggccaggaa
     3241 cttgcagggt ggccggggcg tctcgccgaa attctgctgg ttgcccttca gggccttgac
     3301 ccgcatgagg ttcccggcct gggtggtggt aatttcaccc ggcatccagc ggtaaccgcg
     3361 gccccgcgtg ttgatatccc aggcgtaaac cagcttggcg ccgttcaatt ccacctcgat
     3421 ggcgtcatcg ctgtccatcc aggcaatcat ccgcagccag acccggtaac ggcccggcgg
     3481 caggacgcgg tccagcttct tctccatcac cgcgcccatc gagaactcgt tggccaccgc
     3541 gtaggcccca ccgagggccg aagtctcacc gtaaaggtgc tccttggcgt accagcggcc
     3601 ggcaaccgtc tgccagatct cgaactcctc caggccgtcg aaatcctcgg cctccacgaa
     3661 gaagccggcc ggtttgtcgg ccgccggcgc cgggccggga ccgattgaaa ggaaacccag
     3721 caggatcgga agcatcaacc acctttgcca cgacatcgcg cctccctggt tgaagagatg
     3781 gttaacaaac ggacttcata cagattgccg tccctgaatt ccctcacaaa ctcaaaccaa
     3841 gaaatccggc gaaattatcc gcgaaatgca tttaaagact ggattcccgc ctactacatg
     3901 cgggaatgac ggcggaaaaa cgcggctttt aatcacaacc tccgccatcc ttgtctttgc
     3961 ttttcgtccg ttccccttaa gcggcggcct tcggtgagcg gcgtcgcgga gtccgggtgc
     4021 aaaacaagcg gaacgaggga atgagtatcc ggggcgcggc ccgccttacc agggccgcga
     4081 acacggagag ccctcgttcc cgagtcgcgt agcttttgcc ccggacgcag agtggccgcg
     4141 aaccgaaggc cgccgcgtca ccataccatg tgtcggttcg gatcgaaatc gagcgccggt
     4201 ttccgataca ggttgctctc cttgaccatc ggccggttgg cgatatccgg gaccgacggt
     4261 tcatagccgg tcagccactc ctcgaccatc ccgtagccgc cgatggcccc gatatccgga
     4321 acaacccggc tgcgcacccc caggttggtc agaagcgtgt tgtagatccg cagcaccttg
     4381 ccccgctgcc aggcgttctc gaactggccg ggctccacct ggaggtagac aatccgaccc
     4441 cgcccgaccg ggataacccc ggccacgcca ttggaaaccg gctggccatc cacagccgtc
     4501 acctgcagca gttccagcgg tcgccggaag gagaagtcgc tggggcccac gccggcaaag
     4561 agcggatcgg ccgggacctc cggcttccag aacttcttct tctccagcgt gaccttcacc
     4621 ggcagcgatt gcaccgccgc cgcatcccgg aaggtcgtca ccagcgtccc gcccccctgg
     4681 agaaacgggc cgagcgcggc cgcctggccg ggggcctccg acaggtcaag gtaggccacc
     4741 cggccgttcc agccaggctg gtaatcaacg gccagttcat ccagccgaat cttcgcccgc
     4801 tccccgcccg ggtgggccag cgccgcccag gcccggcccg agggctgccg gtaatcccgg
     4861 atgatccgct ccagcaggtg cgtcgccacc gggtcgacgc caaaccggtc aaccagttcc
     4921 agctggcaga agagaatccg ccccttgccc tggtagaatt ccaccagcgg cgtgtagagc
     4981 aggtcaaatc cctcgtccat cagaacccgg aagttaccca gctgcggctt ctcgaaggtg
     5041 aaggtggcca cgatcccctt gttggaccag tgccagaagc ggcgctggcc aaaggagttg
     5101 ttctggccgt acttgtagga gttgtcgggc tcggtgctct cgtgcagggg cgggtaggcc
     5161 ggcagcgacc ggctggctcc ccgccagtcg tagaagtcct cgttgaccag gtcggccagc
     5221 agcgccgagg cgccgttccg gatgaaggcc cggcggtcct tgaagctctc cacgctcagg
     5281 ccggccacct gccggttgga ctgctccagg atcaccacgt tcaacccctc cgccaccgcc
     5341 tggccgatcg gcaggctctc cttgaagagc ctggccgcct ccgggtaact gttgcggccg
     5401 atgaccagca gcgggtaacc ggccagggaa ctccggccgc ccgcttccgc cgtcttctcg
     5461 ctgaaaagct ggtagggcac gcccagcttc tccagggccg ccgccgtctc gccccggctg
     5521 tcgatcaggg ccacccggcc gccggccgcg gcggccgccg ccggccgcgg gaagaccgtc
     5581 agggcgaacc gatcgctgac atccagtttc ccgtcatcgc tcttgaaggc ggccgagatt
     5641 tcgacaccgg tcttgccggc cagaccggcg gtctcgaaac ggagcggctg aaagtgcgtg
     5701 ccaccggccg gaaccgtgac gtcggccgag ccggaggcca gcgtccggcc gccgccggcc
     5761 gcctgccagc tgatccggcc ggagacgggg tgaaaattgt cgttgacgat gatggcgctc
     5821 ttctcgaccg tttcgccggc gaagtaattg tggtccttgc tcacgaaatc agccgccggg
     5881 ccgcccaggt agaagaggaa aggcagattg acccgctgca gcgtttcgcc gaaccggccc
     5941 agtttggatc cgtaatagat ctcgccgagc ttgaaggtgc cgtggaagag gtagccgctg
     6001 atgccgtagg tgcgccagga gcgcagcacc cgggtgtagg ccaggtcgta aacgtcgttc
     6061 cggatttcgg cgtcggagtt gatccaggcc gagatcttcg cggccgggtc gagatgcgcg
     6121 tcgatcatcg gctcggtcac cttcgagtag ccggtgtccc cgaagtagcg ggcgtgctgc
     6181 tccagcagga tgatctcctt gctccccttc tgccagagaa tggcctcgtg atggatggga
     6241 accacctgca tctcggtcgg caccagcgga tccggccggt tcttggccca gaccgtgagc
     6301 cactcctcgc tctcctggat cggcgttccg aagcccaggg tggtcatgat accccggaaa
     6361 tcgccgccgg ccccgagccg atagtagaag gtcaggcggc cggtcggatc aatcccgtcc
     6421 agggcccggt tctgctggta ggcccagtta tcgcgctgga tctcctcggg cacgatgccg
     6481 ccgacctggg tcgggtggcc atgtttgccg ttgacgtagc cgttgcccca ccattgccag
     6541 gcgatgatcg aggggtggtt gccgacctgc ttcagcaggg tctcccactc ctcccgggag
     6601 gtttccaggt catccgggtt gacgccgggg gccatgtaga agaccatcca gccggtctcg
     6661 tcggccgtct ggaggctgtt gatggccggg taaacgggcg agagggtccg gtcggcattc
     6721 tggccgaccg cctgcaggcg ttcgatttca tccctggtga atccataacc ggcgagataa
     6781 ttatgcgact tgccccggat ggataatttc accccgttga ggtagatatt tccgtcggcc
     6841 acccagagct cccggtagcc gaacgattcc gggaaggaga gatcaaccgg ctgcccgccc
     6901 ttgccgatct ccagcaccag ccggtagagg ttcggcgtct ccggggacca ggtcttcagg
     6961 acggcggccg gcagggttac cgtccggacg ccctggaaat ccttgggcac cttgagagag
     7021 acgctgccca gctccacggc ctgcttcggg tcgcggtaat ccaccgcccg gcccgcaacg
     7081 accaggtcca gggcgctccc gccggtattc tccaccggca gctgcaggtt gagcgcctgc
     7141 ttccggaagg aggggcagat aagcggatcg cccaggtaga agtccttcgt ctcccgggcc
     7201 tcgatgcagg gcgtcatttc aaagccggtc ggcgtcatcc ccggcgcgaa cggcagcccg
     7261 ttgagcaggg tgatccggtt cggacggtcc cagagcagct gccgggtgat caggaagcgt
     7321 ttttcgccgg aacgggccac cgaacccagg tgcttcccgt tcaggtagac ctcggccgga
     7381 ccgcccaggc gggggaaagt aaggtaaaat tggtagttct tcagccgcgg cggcaggttg
     7441 atgtcccgct ccagccaggc ggccggcagc ttattaaagg ttttgccctg ccagctggcc
     7501 accggtttgc cggtctggtc caggaccgcg ttggccgccc gccagctgcc cggcgccttc
     7561 acataaagcc actccggacc gaagaggccc gggttgacca gctccttatc ccgcacctcc
     7621 ggcaccgccg gctggaaacg ccagagaccg tccaggtcgt atcggaaccg cttgccgttc
     7681 tcggccgccg gctgcagcac cagccacccg gccgccgccg gcaactccgg acggaccgac
     7741 ggcagctggc cgatctcaag cggcagcacc ggatagtcgc gccgccccgg ctccgccagg
     7801 gagaagaccg ccttgccctg ccggtacact ccgtaggcat ccttaacctc ggccgcgctc
     7861 agcggccggt ccagaatcac cacctcgtcg agggcccccg gcaggcggaa accgaagatc
     7921 agctccttcg gccggtaggg tttcaggtaa tcgccctcgg ccaggagggt cccgtccagg
     7981 tactgccagc gcttcctggc cgcgccgtcc cagcagtaaa ccagctggtg ccactggccg
     8041 tcggcaaccg cctcggtgcg cgggtcgttc aaacccacca gaaccaggtc gtgcgcggcg
     8101 gcctccaccc gctccccggc cccgacgcac cagatcagcg ggtagtaaga gttgacgaag
     8161 aagggacggg tgatccgcac ccagagggag atcgtgccgg actgggaatc gtagtttttc
     8221 tcaaccgggt aggcgactcc cggataaccg ttcttccagg tgaagcgggc cccctggccg
     8281 accacgccct tctcgtactc gaagatcgtc agcggcgcgc ccttgctggt gaagaagttg
     8341 gtatagaccc ggtcgcggcc gaaaccgtcg gcccgcttct caaaacccgc ctggaagagg
     8401 acctgcggcg gcgccgacca ggcggtgttg caactgaaga gaccaaggag ggaaagaagc
     8461 atcagcaggg atgggcgcag aaaccgtctg gtcgcattca tcgggcctcc ttgagcggaa
     8521 aggatcggat tggacaccgg gtgctggcct tcggaaaatt tattttaaca ttgccgccag
     8581 taactgtcta ctcccggaca agatatagac aagcgtaaac atcttcaacc tccggccgcc
     8641 tttttgatga ccgtatcggc catcagatat aatgtaggtt aagtttccgg ccggtttcat
     8701 tgaaaaaaac ggagggaccc atgcgcaacg cttcacaggc catctggctc tggagccatg
     8761 ttgagaatct ggattggagc aagtggggcc tggaccgcac gagccggata accccggccg
     8821 ccgcggccga attcctgggc atcgagaatc ttctgctggt cacctatgca gaccagccgg
     8881 cgccgccctt ccgggagtac gcggccggac tggccggatt gaaacaggtc gtctggtcgg
     8941 ccctgggcgc cgccaactgc cggcgcgggt ctgatctttc agcggtgctg gaactggtgc
     9001 cggaaatgaa gaacctgacc ggcgtaatcc tggacgattt cttcaatcgc cgtgaccggg
     9061 acggcaatcc cggcacctac acccgcgccg aactcatgga gatgcggcgc cggctggcgg
     9121 caaccggccg gccgctggaa ctctggggcg tgctctacaa tcaccagctg gagctgccgg
     9181 tggccgagaa gatttccgcc tgggacgcgg taaccttctg gcactggacc gccgaggagc
     9241 tggagcgact ggaaaccacc ctgccccgct tcgaaaaact ggccgccggt cgacgcaaac
     9301 tgctcggcct ctacatgtac gatttcggca accgccggcc gctgccgtta tccctgatgg
     9361 aaagacagtg ccggatcggg cgggacctcc tggccgccgg ccgcatcgat gggctcatct
     9421 tcctgtcctc catcctcggc gatcaaaatc tggaagcggt tgaattcacc cgtgggttcg
     9481 tgaaggaaaa tttccggccc tgaagaaaaa ccggcgtttc cgccccggaa aacccttgat
     9541 caccggaact tattggccca aatacaagga gcacgcatgg atcagaaccg ggctcgtacg
     9601 gacctgaacg gcaagtggtg gattttcttt gaccgggagg acctgggcat ca
//
LOCUS       DAOQYN010000052         9507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_7703_length_9507_cov_4.944668, whole genome shotgun sequence.
ACCESSION   DAOQYN010000052 DAOQYN010000000
VERSION     DAOQYN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7703_length_9507_cov_4.944668"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PKN80_03295"
     CDS             <1..554
                     /locus_tag="PKN80_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ35070.1"
                     /translation="SPRIGGNTDLLLERFLDGCRESGARVEKVILNQLRFSPCQECAR
                     VRDDGSCQVDDQLQPVFRLVGAAGLVVVATPIFFGSLSAQTKMMVDRFQCWWRAKYLL
                     KRDPCPGSRSGVFLAVEASDREDFFDSARAVTRNFFATIGARWQGEVLCPGLESAGEI
                     LSRPECLEAAFQLGRRLASEPAA"
     gene            662..1150
                     /locus_tag="PKN80_03300"
     CDS             662..1150
                     /locus_tag="PKN80_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNQ35071.1"
                     /translation="MMEKKLAAALNEQINHELFSAYLYLSMAAWSETRNLPGFAHWLS
                     LQAKEEVGHAMKIFGFLVDRGERVVLDAIKKPETDFESPRDVFARTLEHEKKVTALIN
                     RLYDLSGKVNDQAAAIFLQWFVTEQVEEEKNASSILETLKMLKPDSSAIFMLDHQLAK
                     RE"
     gene            1238..1990
                     /locus_tag="PKN80_03305"
     CDS             1238..1990
                     /locus_tag="PKN80_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNQ35072.1"
                     /translation="MAEIPARLPFEERLRLWHGEIWHTARGRIIRDIVYAIDTGLVTT
                     VSFLAGISISLVEKERVIAASLIQVASGTLAIFFGSYISTKAQKHFFENQIERERREI
                     EEKPEKERQEIRQVMSEFGFTGEEQEMAVRRITADKECWLRFMVQEEIGISPGMIDNP
                     VEIGFVSAFSFLVGALPALLPFFLLASVKTALAVSALTVFLFLFSLGVFKARITKIRW
                     LYSGLETLFFGLLSSGSGFLMGRMINLYFLRN"
     gene            1990..2469
                     /locus_tag="PKN80_03310"
     CDS             1990..2469
                     /locus_tag="PKN80_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ35073.1"
                     /translation="MAKKTQRFMVWLLPTVVIGGLFWPPLGFLVFGMMLFMLVLAVFR
                     GRYWCGNLCPRGAFLDLVLSRFVSGRAYPRLLTGGRLRWPVVILFFLFFAYRLATVPR
                     TLYGFGFLFVQMCLLTTVLAIIMGLATRARAWCVICPMGTIQAKLGSLKRPRPGSGG"
     gene            complement(2504..2662)
                     /locus_tag="PKN80_03315"
     CDS             complement(2504..2662)
                     /locus_tag="PKN80_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNQ35074.1"
                     /translation="MGKYRCQVCGYIYDPAAGDPDNGIKPGTSFDDLPDDWVCPECGV
                     GKDQFEPI"
     gene            complement(2697..3314)
                     /gene="wrbA"
                     /locus_tag="PKN80_03320"
     CDS             complement(2697..3314)
                     /gene="wrbA"
                     /locus_tag="PKN80_03320"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNQ35075.1"
                     /translation="MKDRTIILALYYSLYGNVFALMKEAAAAAAEVPGTEVRLRRVPE
                     LIPEAVIRENEAMSKARARQADVPLAGLDELAGCDGLLLGAPTRFGNMCSQMRNFLDQ
                     TGGLWQKGALAGKPAGLLTSTNTMHGGQETTLVSMAFTLLHHGMVIVGVPYTVPELST
                     TRGGGTPYGASRLAGEAGSPPSPEETAIARALGKRLAEIAVRLKG"
     gene            complement(3311..3448)
                     /locus_tag="PKN80_03325"
     CDS             complement(3311..3448)
                     /locus_tag="PKN80_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HNQ35076.1"
                     /translation="MANVQNRGEVYRCEVCGNVVQVLEVGGGELVCCDQPMTPVKKEE
                     K"
     gene            complement(3460..3849)
                     /locus_tag="PKN80_03330"
     CDS             complement(3460..3849)
                     /locus_tag="PKN80_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HNQ35077.1"
                     /translation="MADGTLKCADDILCGINRPKDPANPSDLEKKHLPVIEAPAQAGK
                     DQPFQVRVEVGKLLAHPNEPAHFIAWLELYSGETFLGRAEFTGGGSYPTAVFTVKLSH
                     GHGALRARALCNLHGLWESERPIEIIS"
     gene            4025..5449
                     /gene="glgA"
                     /locus_tag="PKN80_03335"
     CDS             4025..5449
                     /gene="glgA"
                     /locus_tag="PKN80_03335"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNQ35078.1"
                     /translation="MKILFAAAEGVPFFKTGGLADVIGSLPPVLSDQGLEVRVALPFY
                     RRLARFEPAARPLLETRLDFAGRRLELRVLAGPKAAVETWFFDAPELFDRDHIYGPPG
                     ADYPDSLLRFGFFSFAVLKVLPELGFRADVAHVHDWHTAALPVYLRELLAADPFYREM
                     KSVLTIHNLGYQGVFSHSNWPMLGLPDHLFNLNGFEFYNQVNLLKAGLLWADRLTTVS
                     PSYSREIQTPEYGEKLDGLLRQRSSSLVGILNGIDYRYWDPASDRMLTQVYDPAQPDG
                     KAVNKADLERRFGLDPGRPLLGVVSRLVPQKGFELVMEALGDILRLGFNLVILGSGLD
                     KYEEFFGRAVQKFPGRLGFQTGYQEELAHRIYAGSDFFLMPSLYEPCGLGQMIAMRYG
                     TIPVVRRTGGLADSVAEFKSGRGTGFLFDQPAAPELLAALGRAHRAFGDARAWRKLRA
                     NAVAADFSWQRSAAEYAGLYRSIS"
     gene            5467..7497
                     /locus_tag="PKN80_03340"
     CDS             5467..7497
                     /locus_tag="PKN80_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNQ35079.1"
                     /translation="MAEPFKVVFLWHFHQPSYLDFDSGRYRMPWVRLHAVKDYYAFAR
                     LLGRHPSVRATFNVTPVLLEQVLDIAGRRPDDDFSELTRRPADQLDEAARLYLARNFF
                     ALNWETMLFPFPRYRQLLEKRGRVSDTEVLERRAGDFSIAELRDLQVLFNLAWVDPGL
                     RREDELPRELERKGRDFTEAEKLGLLDYHYQLAGRVIPEWRRLQDSGQVEFSVSPYYH
                     PILPLLLKGPHPLPEDASLQLKEARLLFRAVFGRDPAGCWPSEGAVSPAALDRLAAAG
                     FRWAASDEEVLGRSLGPAFRRELIYCSHEYRGPSGSIRMLFRDRDLSDRIGFVYRFQA
                     ADRAAADFSAGLERARSAAGPGAAVPVILDGENAWEYYPENGRPFLEALYRSLESNPA
                     FPTATVSDYLDASTPVLLERIHSGSWINGDFHIWSGHPAADRAWQALADARSGVDLAA
                     AGPELRRSLLAAEGSDWFWWYSPEHFSGRDEEFDAAFRGLLRNAYRLGGRPVPADLEQ
                     PIKCGQEEVHFPRNPVTAVLDGRETSYYEWLGAGHFLRHLAGGAMHGPPEAVTEFLFG
                     WDREHLYLRIEPGPLLREAERSFFKLDFGPAGCWQFEARQGKINPDSCPFPVAQSELI
                     EIAFPWKELDRPAGAGISFCLEITAGSQVLDRYPRQGGLELRVPGEDYETVF"
     gene            7557..9050
                     /locus_tag="PKN80_03345"
     CDS             7557..9050
                     /locus_tag="PKN80_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HNQ35080.1"
                     /translation="MKGTRVGLVLINDEREHVYRSNEAESLKVVSAWADFIGREAVNL
                     DGSRVETVCASETVKSVRTAQKLGAELAAAGCQQVIFIYNVWCFPFLVWPFLNSFGRD
                     KPILSLSNNSGEFPGNVGLLATDGALRQAGFRTHRLVGDQADPATRAAVLAWVRASQA
                     VTTIRNEVFGAYGGPSMGMDTGYFHLVPNLKTLGTSVQFIDQLWLVEKMKEVSEAEVE
                     AGFAWFGRMLGNRILYDGKMLTPETLKTQLRLYLAMEAVNRDKGFDFCGLKGQRELTE
                     YVCLGDIPEMLMNDPYDWRGPKEPMVCATEADAYAALTMQLLKYLSGGRPTLFMDVRL
                     YHPDLDLWDFCNSGNHSTYYAALSTDPAVNYRKVTFFPALEYYFKAGGASMFYDAAPG
                     RLTFARLGLWDDRPFLVIVPGESVELPAANRKRLDDQTNPTWPHVHARLECGFEEFKT
                     VFPANHILAVAGDHVSALVYAAEILGIPPVLLGPAAAGRVRPIWERV"
     gene            9050..>9507
                     /locus_tag="PKN80_03350"
     CDS             9050..>9507
                     /locus_tag="PKN80_03350"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNQ35081.1"
                     /translation="MAVVKPFRGTFYSPGLDPSRVLAPPYDVISPEQRRDLLARDSHN
                     VVRLILPGTNEAAARDFRAWRAGGIFVTDPAPGFYWCRQEYTLDGRSAVKTGLIGILK
                     TEPLGRGSVRPHEETIPQVKEEQFRLLGLVRANFCPIITVYRDSRRFVEKL"
BASE COUNT         1484 a         3100 c         2997 g         1926 t
ORIGIN      
        1 gcagcccgcg gatcggcggc aacaccgacc tgctgctgga gcgctttctt gacggctgcc
       61 gggaatcggg ggcccgggtg gagaaggtga ttctcaacca gttgcgtttt tcgccctgcc
      121 aggagtgcgc ccgggtccgg gacgatggct cctgccaggt cgatgaccag ctgcagccgg
      181 tcttccggct ggtcggcgcg gccggcctgg tggtggtggc gacgcccatc ttctttggaa
      241 gcctgagcgc ccagaccaag atgatggtgg accgtttcca gtgctggtgg cgggccaagt
      301 acctgctcaa gcgcgatccc tgtccgggta gccgctccgg tgtcttcctg gccgtcgagg
      361 cctcggaccg ggaggatttt ttcgacagcg cccgggcggt gacgcgcaac ttcttcgcga
      421 ccatcggcgc ccgttggcag ggcgaggtgc tctgtccggg gctggaatcg gccggagaga
      481 tcctctcccg gccggaatgc ctggaggcgg cctttcaact gggccggcgc ctggcctccg
      541 aaccggccgc ttgaattact tccgccattt ttggcggcgg ggcctccggt atggtaaaat
      601 ctaaatagag agcaaatact atttaaaaat tcggctgctt attttcaagc cggggaggcg
      661 gattatggag aagaagctgg cagcggcgct taacgagcag atcaatcacg aattgttttc
      721 ggcctatctt tacctttcca tggcggcctg gagcgagacg cgcaacctgc ctgggtttgc
      781 ccactggctg agtctgcagg ccaaggaaga ggtcggccac gccatgaaga tcttcggctt
      841 cctggttgac cggggcgaac gggttgtcct ggatgcgatc aagaagcctg agaccgattt
      901 cgaatcgccg cgggacgtct tcgcccgcac cctcgagcac gagaagaagg tgacggcgct
      961 catcaaccgg ctctacgatc tttcgggcaa ggtaaacgac caggcggccg ccatcttcct
     1021 ccagtggttc gtgaccgagc aggtcgagga ggagaagaac gcttcgtcca tcctcgagac
     1081 gctcaagatg ctcaagccgg attcctcggc catcttcatg ctcgaccacc agctggccaa
     1141 gcgcgaataa ggatcgccag gaatttcaaa cgaaggggag gggggctgcg gaaaacatcc
     1201 ccgctcccct tctttttaac cattcagaga ccagaccatg gccgaaattc cagcgcgcct
     1261 tccctttgag gaacggctcc ggctctggca tggcgagatc tggcataccg cccgggggcg
     1321 catcatccgg gatatcgtct atgccatcga taccgggctg gttaccaccg tttccttcct
     1381 ggccggaatc tcgatttccc tggtggagaa ggaaagggtg atcgccgctt cgcttatcca
     1441 ggtggcttcg ggcaccctgg ccattttctt cggttcctac atctccacca aggcccagaa
     1501 gcattttttt gaaaaccaga tcgaacggga gcggcgcgag atcgaggaga agccggagaa
     1561 ggagcggcag gagatccgcc aggtgatgag cgagttcggc ttcaccggcg aggaacagga
     1621 aatggccgtc cggcgcatca ccgcggacaa ggagtgctgg ctgcgcttca tggtccagga
     1681 ggagattggc atcagccccg gcatgatcga caacccggtc gagatcggct tcgtttcagc
     1741 cttctccttc ctggtcggcg ccttgccggc cctgcttccc ttcttcctgc ttgcctcggt
     1801 caagacggcc ctggccgtct ccgccctcac ggtcttcctc ttcctcttca gcctgggcgt
     1861 cttcaaggcc cggattacca agatacgctg gctttacagc ggcctggaaa ctctcttctt
     1921 cggtttgctc tcttcaggca gcggattcct gatggggcgg atgatcaacc tttactttct
     1981 gaggaactga tggccaaaaa aacgcagagg ttcatggttt ggctgcttcc gacggtggtc
     2041 atcggcggtc tcttctggcc gccgctgggc ttcctggtct tcggcatgat gcttttcatg
     2101 ctggtactgg cggtcttccg ggggcgttac tggtgcggca acctctgccc gcgcggcgcc
     2161 tttctggacc tggtcctcag ccgtttcgtc tccgggcgcg cctacccccg gttgctgacc
     2221 ggcggaagat tgcgctggcc ggtggttatc ctcttctttc tcttcttcgc ctaccggctg
     2281 gccaccgttc ccaggactct ttacgggttt ggtttcctct tcgtccagat gtgcctgctt
     2341 accaccgtcc tggccatcat catgggattg gccacccggg cgcgggcctg gtgcgtgatc
     2401 tgcccgatgg gaaccatcca ggcgaagctg ggctccctga aacgtccccg ccccggaagc
     2461 ggcggttgac caggctggcg gccgccgctc ccggggcgtt atttcagatc ggttcgaact
     2521 ggtccttgcc caccccgcac tccgggcaga cccagtcgtc gggaaggtcg tcaaacgagg
     2581 tacccggctt gatcccgttg tcgggatcgc cggcggcggg gtcgtagata tatccgcaga
     2641 cctggcaacg gtattttccc atcctgtctc cttccggttt caatttgtcc cggcggtcag
     2701 cctttcaagc ggacggcgat ttcggccagc cgttttccca gggcccgggc gatggcggtt
     2761 tcctccggag agggcggact acctgcctcc ccggccagcc ggctggcccc gtaaggggtc
     2821 ccgccgcccc gggtggtcga aagttccggg acggtgtagg gaacgcccac gatcaccatg
     2881 ccgtgatgga gcagggtgaa ggccatcgag accagggtgg tctcctgtcc gccatgcatg
     2941 gtgttggtcg aagtgagcag cccggccggt ttgcccgcca gcgctccctt ctgccagaga
     3001 ccgccggtct ggtccaggaa gttgcgcatc tggctgcaca tgttcccgaa acgggtcggc
     3061 gctcccagca gaagcccgtc gcacccggcc agctcgtcga gaccggccag ggggacatcg
     3121 gcctgtcggg cccgggcctt cgacatcgcc tcgttctccc ggatgaccgc ctccgggatc
     3181 agttccggca cccggcggag ccggacttcg gtccccggca cctccgccgc cgcggccgcc
     3241 gcctccttca tcagggcgaa gacatttccg taaaggctgt agtaaagggc caggattatg
     3301 gtcctatcct tcatttctcc tctttcttga ccggggtcat cggctggtca cagcagacca
     3361 gttcgccgcc gcccacttcc aaaacctgga cgacgttgcc gcagacctcg cagcggtaca
     3421 cctcgccgcg gttctgaacg tttgccattg gttcctccct caggagatta tttcaatcgg
     3481 gcgttctgat tcccagagtc cgtgcaggtt gcagagggcc cgggcccgga gcgccccgtg
     3541 gccgtgggag agcttgaccg tgaagacggc cgtcgggtag ctgccgccgc cggtgaactc
     3601 ggcccggccc aggaaggttt cgccgctgta aagctcgagc caggcgatga aatgggccgg
     3661 ctcattcggg tgggccagca gtttcccgac ctcgacccgg acctggaatg gctggtcctt
     3721 cccggcctgg gcgggcgcct cgatcaccgg taggtgcttt ttctcgaggt cgctcggatt
     3781 ggccgggtcc ttcggccggt tgatcccgca caggatatcg tccgcgcact tcagcgtacc
     3841 gtcagccatg aaggcctccc tggttggcgt gcacaagccc ggtttcgccc ggcatctcgc
     3901 cggtacatat agttttaccg aaagggcgcc ctttgtcaag gcggccgctc ccgggtcgat
     3961 ttttcaataa tggcgcgccc tccgcttttg taaggtgcct cccgggtatg ctaaacttaa
     4021 agtcatgaaa atactatttg ccgcggcgga aggagtccct tttttcaaga ccggcgggct
     4081 ggccgatgtt atcggctccc tgccgccggt actctcagac caggggctgg aggtgcgggt
     4141 ggccctgcct ttttaccggc ggctggcccg gttcgagccg gcggcccggc cgctcctgga
     4201 gacccgcctt gattttgccg gccgccggct cgagctccgg gtcctggccg ggcccaaggc
     4261 cgccgtcgag acctggtttt tcgacgcccc ggaactcttc gaccgcgacc atatctacgg
     4321 gccgcccggg gccgattacc cggacagcct gctgcgtttc ggtttcttct ccttcgcggt
     4381 gctcaaggtc ttgccggaac tgggtttccg ggccgatgtt gcccatgttc acgactggca
     4441 taccgccgcc ctgccggttt atctcaggga gctcctggcc gccgatccct tctaccggga
     4501 gatgaaatca gtcctgacca ttcacaacct cggttaccag ggtgtttttt cccattccaa
     4561 ctggccgatg cttggcctgc cggaccacct cttcaacctc aacggtttcg agttttacaa
     4621 ccaggttaac ctgctcaagg ccggcctgct ctgggccgac cgcctgacca cggtgagtcc
     4681 ctcctacagc cgggagatcc agacgccgga gtacggtgaa aaactcgacg gcctcctccg
     4741 gcagcgaagc agcagcctgg tcggaatcct gaatggcatc gactaccggt actgggatcc
     4801 cgcttcggac cggatgctga cccaggttta cgacccggcc cagccggacg gcaaggcggt
     4861 caacaaagcc gatctcgagc gccggttcgg cctggatccg ggccgcccgc tgctgggcgt
     4921 ggtcagccgc ctggtccccc agaagggctt cgagctggtg atggaggccc tcggagatat
     4981 tctccggctt ggcttcaacc tggtcatcct gggcagtggc ctggacaagt acgaggagtt
     5041 cttcggccgg gcggttcaga aattcccggg ccgcctgggc tttcagaccg gctaccagga
     5101 agaactggcc caccggattt atgccggcag cgatttcttc ctgatgcctt ccctctacga
     5161 accctgcggc cttggccaga tgatcgccat gcgttacggt accatcccgg tggtccggcg
     5221 gaccgggggt ctggccgact cggtggccga gttcaagtcc ggccggggta ccgggttcct
     5281 cttcgaccag ccggcggccc cggaattgct ggccgcgctc ggccgggcgc accgggcctt
     5341 cggcgacgcc cgggcctggc gtaaactccg ggccaatgcc gtggccgccg atttttcctg
     5401 gcagcgttcg gccgccgagt acgccggcct ctaccgctcc atctcctgac ccgggctttt
     5461 tgagcgatgg ctgagccttt caaggtcgtc tttctttggc actttcacca gcccagctac
     5521 ctggatttcg attccgggcg gtaccggatg ccctgggtgc gcctgcacgc ggtcaaggat
     5581 tattacgcct ttgcccgtct cctggggcgc cacccgtcgg tgcgcgccac tttcaatgtt
     5641 accccggtgc tcctggaaca ggtcctggat atcgccgggc gccggccgga cgacgatttc
     5701 agcgaactca cccgccggcc ggccgaccag ctggacgagg cggcgcgcct ttatctggcg
     5761 cgcaatttct tcgccctgaa ctgggagacg atgctgttcc cgttcccgcg ctaccggcag
     5821 ctgctggaga agcgcggccg cgtctccgat accgaggtgc tggagcgccg ggccggtgat
     5881 ttttccatcg ccgaactccg cgacctgcag gtactcttca acctggcctg ggtcgatccc
     5941 ggactgcgcc gggaggatga gctgccccga gagctggagc gcaaggggcg ggatttcact
     6001 gaggcggaga agctcgggct gctcgattac cattaccagc tggccgggcg cgtcatcccc
     6061 gagtggcgcc ggctgcagga ttcgggccag gtcgagtttt cggtctctcc ctactaccac
     6121 ccgatcctgc ccctgctgct gaaggggccg catccgctcc cggaagacgc ctcgctccag
     6181 ctgaaggagg cccggcttct cttccgcgcg gtctttggac gggacccggc cggctgctgg
     6241 ccttccgagg gcgcggtcag cccggccgcc ctcgaccggc tcgctgccgc cggttttcgc
     6301 tgggcggcct ccgacgagga ggtgttgggc cggagtctgg gcccggcctt ccggcgcgaa
     6361 cttatttact gcagccacga ataccggggt ccgtccgggt ccatccggat gctattccgc
     6421 gaccgggatc tttccgaccg catcggtttc gtctaccgct tccaggcggc cgaccgggcg
     6481 gcggccgatt tcagcgccgg cctggaacgc gcccggtcag ccgccgggcc cggcgccgcc
     6541 gtgcccgtaa tcctcgacgg cgaaaatgcc tgggagtatt acccggagaa tggccggccc
     6601 tttctcgaag cgctctatcg ttcgctggag tcgaacccgg ctttcccgac ggccaccgtg
     6661 agtgattacc tggacgcttc gacgccggta ctgctcgagc ggatccactc cggttcctgg
     6721 attaacgggg atttccacat ctggtccggc cacccggcgg ccgaccgggc ctggcaggcg
     6781 ttggccgacg cccggtcggg cgtcgacctg gccgcggccg ggccggaact gcggcgttcc
     6841 ctgctggcgg ccgaggggag tgactggttc tggtggtatt cgcccgagca cttttccggc
     6901 cgcgacgagg agtttgacgc cgccttccgc ggcctgctcc ggaatgctta ccgccttggc
     6961 ggccggccgg tcccggccga cctggaacag ccgatcaagt gcggccagga agaggtgcat
     7021 tttccccgca acccggtgac cgcggtcctg gacgggcggg agaccagtta ctacgagtgg
     7081 ctgggcgccg gccattttct gcgacacctg gccggcgggg ccatgcacgg tccgccggag
     7141 gccgtcaccg agttcctctt cggctgggac cgggagcatc tttacctgag gatcgagccc
     7201 ggtccgctgc ttcgggaggc cgagcgcagc ttttttaaac ttgatttcgg tccggccggc
     7261 tgctggcaat ttgaggcccg gcagggtaaa ataaatcctg attcctgccc atttccggtg
     7321 gcccagtccg aactgatcga gatcgccttt ccctggaagg agctggaccg gccggccggt
     7381 gccgggattt cattctgcct ggagattacc gccggcagcc aggtacttga ccgttacccg
     7441 cgccagggcg ggctcgagct ccgggtgccg ggtgaggatt acgaaacggt gttttgaatt
     7501 taaaccgggc tcttttttcc ccctttccat tccgtgttaa aatcaggagg ccgcgtatga
     7561 agggtaccag ggttggcctg gttttgatca acgacgagcg ggagcatgtt taccgttcca
     7621 acgaggccga atcgctgaag gtcgtcagcg cctgggccga ttttatcggc cgggaggccg
     7681 tcaacctgga cggcagccgg gtggagacgg tttgcgcctc ggagacggtc aaaagcgtgc
     7741 ggaccgccca gaaactgggg gccgaactgg cggcggccgg ctgccagcag gtgatcttca
     7801 tctacaacgt ttggtgcttc ccctttctgg tctggccctt tctgaacagc ttcggccgag
     7861 acaagccgat cctgagcctt tccaacaaca gcggcgagtt ccccggcaat gtcgggctcc
     7921 tggccaccga cggcgccctg cgccaggccg gtttccggac ccaccgtctg gtgggtgacc
     7981 aggccgatcc ggccacccgg gccgcagtcc tggcctgggt ccgcgccagc caggcggtaa
     8041 ccaccatccg caacgaggtt ttcggggcct acggcggacc ctcgatgggc atggataccg
     8101 gctacttcca cctggttccg aatctcaaga cgctggggac ctcggtccag ttcatcgacc
     8161 agctctggct ggtggagaag atgaaggaag tgtcggaagc cgaggtggag gccggtttcg
     8221 cctggttcgg caggatgctg ggcaaccgga tcctttacga tggcaaaatg ctcaccccgg
     8281 agactctcaa gacccagctc cggctttacc tggccatgga ggcggtcaac cgtgacaagg
     8341 gattcgattt ttgcggcctg aaggggcagc gcgaactgac cgagtacgtc tgcctgggcg
     8401 acattcccga aatgctcatg aacgaccctt acgactggcg cggtcccaag gaaccgatgg
     8461 tctgcgccac cgaggccgac gcttacgcgg ccctgaccat gcagctgctc aagtacctga
     8521 gcggcgggcg gccgaccctc ttcatggatg tccggcttta ccatcccgac ctcgacctct
     8581 gggacttctg caactccggc aaccacagca cctattatgc cgccctttcc accgacccgg
     8641 ccgtgaatta ccggaaggtt accttcttcc cggccctcga gtattatttc aaggccggcg
     8701 gcgcctcgat gttttacgat gcggcgccgg gacggttgac cttcgcccgg ctcggcctct
     8761 gggacgaccg gcccttcctg gttatcgtgc ccggtgagag cgtggagctg ccggcggcta
     8821 accggaagcg gctggacgat cagaccaacc ccacctggcc ccacgtccac gcccgcctgg
     8881 aatgcggctt cgaggagttc aagacggtct tcccggccaa ccacatcctg gccgtggccg
     8941 gcgatcacgt gagtgcactg gtttacgccg ccgagattct cggcataccc ccggtcctgc
     9001 tcggtccggc cgcggccggc cgggtgcggc ccatctggga aagggtctga tggccgtcgt
     9061 caagccgttc cgcgggacct tttactctcc cggcctggat ccgtcccggg tgctggcgcc
     9121 gccttacgac gtcatttctc cggagcagcg gcgggatctg ctggcccgcg attcacataa
     9181 cgtcgtacgg ctgatactgc ccgggacaaa cgaggcggcg gcgcgcgatt tccgggcctg
     9241 gcgcgccggc gggatcttcg tgaccgatcc ggcccccggt ttttactggt gccgtcagga
     9301 gtacaccctc gacggacggt cggccgtcaa gaccggcctg attggcatcc tcaagaccga
     9361 gcccctcggc cggggctcgg tgcgcccgca cgaggagacc ataccccagg tcaaggagga
     9421 gcagttccgg ctcttgggtc tggtccgggc caacttctgc ccgatcataa ccgtttaccg
     9481 cgattcaagg cgtttcgtcg agaaact
//
LOCUS       DAOQYN010000053         9480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_7731_length_9480_cov_4.727745, whole genome shotgun sequence.
ACCESSION   DAOQYN010000053 DAOQYN010000000
VERSION     DAOQYN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7731_length_9480_cov_4.727745"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="PKN80_03355"
     CDS             <1..1740
                     /locus_tag="PKN80_03355"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HNQ35082.1"
                     /translation="EEGQDTSDTDGADELLVVYAPEAQAFGLASGVAANDGDLLLHFG
                     ATTGWRLDENWGDALVRFLTSWPVRLLLEGPGGKQEWRRETGPGGVVAVEGVKVDKPG
                     VYFARAAAAGDEKVAGSSNPMLCADSGEGVFWGDCHCHTGWSDGIGTVEDNLLFARDR
                     AFLDVFGFAEHLAVKDFTTQPVDKGGSDWAYLGPTMAEASNRWNAAGKFVTMLGYEYT
                     PGKTVRDPSGDCCVFSRGGSWSDLPAATELNDLLALSKAAGCLVIPHVGGRTPKWDKV
                     AFAPETTPLVEIASMHGKFEAFAQAGLQQGYKLGFCGMSDGHYGKPGYDCWALHGRIR
                     SLKERNFSVQSAITAFLCRELTREAVFESLRRRRTYATTGERIFLRFEVNGASMGESL
                     VTRERPEIHIRVAGSAPISRIDLIRGGRRVAMWEFKGEKEAEIRWIDKAPSPAETYYY
                     ARITQADFANAWSSPVWVAYTGPDPAGPEQESQLPEWNAAPEWPASTGLEVPRDLLAL
                     QLEVFERKGIADRFPDLEPVGIFSENRGRFVFYRGYDRETGSRIHCQHYFEFDDDRLL
                     ISPGWSPYGQVLY"
     gene            1890..2399
                     /locus_tag="PKN80_03360"
     CDS             1890..2399
                     /locus_tag="PKN80_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35083.1"
                     /translation="MIKKVGFFLRSGVRLSVANLLLALLVLLLCFSTIVIIFRSEILQ
                     LKRDKKVLHEKVLNLIETGTASPDVIKRWISRPPQLSSKEIQYLKKRGLKDPVNDIAE
                     DLMKHNEFIPYRGIVGGKMQFFEVYVISTRLVRAYFEDGHIGGWMMLEYRVNKGGRIS
                     WEVLESYLN"
     gene            2497..3924
                     /locus_tag="PKN80_03365"
     CDS             2497..3924
                     /locus_tag="PKN80_03365"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNQ35084.1"
                     /translation="MISRVLSEEQVEKIHRASLAVLERVGVAVPHPEVLGRFHDSGAA
                     VDEKTQRVKIPPDLVMRLLKQAGKEFTIYGRDLSRKAGFGKGTRNYNASGGQASWVEE
                     PGRERRYATLTDVATAARFCDGLERINIAGAMADPHEIPVHYRCVEVAAAMLENTTKP
                     LTFWFYDRPSARHIVEMLILLRGSAEKAAEFPLCYPFLEPISPLSFPFHGIDLIFETA
                     RLNLPVPVGPMAQTGLSAPGTLAATMVQENAEILAGICITQLIRPGLPVCYGGICHVF
                     DMATTQMVFGGPEQAIFGVGMTQLGKHYGLPVYINVGLTDAKSVDAQAGLEAGVTLAL
                     GAAAGADIFGHLGICGVDQGASLDLLVFQAEMISYVEHLLRDIDFSDEALGIDEIETV
                     GPGGIYIDREFTAGRFRKELWFPKLLDRRYYQAWLESGARGAAENCRRRKEEILRSHA
                     PEPLAPELKLGLAEIVAAAKKDLTG"
     gene            3991..6711
                     /locus_tag="PKN80_03370"
     CDS             3991..6711
                     /locus_tag="PKN80_03370"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNQ35085.1"
                     /translation="MFVIAVIVILLLLIFLPGRRTSPEPPQRPASQADELKFLLRRVA
                     DLERRLESLEGKAPPAGPAAETPGREEPAAAVATPPSGPETAIPLAARMGPAAARTVG
                     TARPDSGQVRRRLLSLERTIGEKWLAWVGSLVFAAGFGLLLKYAFEQGWLNEWARTGL
                     GFAAGLFFLLLSELLYRKSYRVLAQGVAGVGSIILYLTVFIAHRFYLFFDTPEASLLF
                     VAVSITGMALAVLHDSYAAALLTVLGAFATPVLLFSSISYLYYYGPAFFSYLLFIDLG
                     VLYVSVVRRWRALFLLAFFGTTGYLGLWYLQKYTGADFRVAILFSILYFILFSFTATL
                     YSLWRRGRSFREDLAFVILNPVFFFIFWYGLLSRQGLDWLLPLVPLAMAAFHYLLARI
                     VRLRNPEDRLLYFALAGTATGLLTLPVPIWIKDSWMTPAWGVEAVILVAVGSFFNRRF
                     LRIGGLAILCLVGYRLALIDPFAARSAGFTAGFIARPFLNLRFLPQLIAAACFGLAAF
                     AFKNLPGLPDGERKFCGRLWLAFAVFLFWIANLELFGYFSRLGAFAESMKWPFSTILW
                     SLFAWWLFFAGMLRRNGRLFKAGLGLAAATVLKVMLLDTPLLRGQGHGYPFLLNLRFL
                     APAFLLGALATAAFFHARLGDGERPGGRRALPWLWTFFTALLFWVLNAEVFAFFGQVE
                     LNAFPESLIFTDILWAVFALVLVRQGLRNGVSALRTAGLLLLVAAFVKAILADLFDGY
                     GLFLSGGDPLLFNLKFLGAAAMLVVAAHLASLYAEPGEGRTYFERPAVPCLWILFLVL
                     LFVELNLQSRLLLQGIWDLERQRFALVLSLLWFLYGFGLLLAGIVKESLPLRFSALTL
                     FGVTLLKVFFVDLNFIGKAYRTAALLAIGSLLLIGAYLYRRYRQRISERE"
     gene            6723..7877
                     /locus_tag="PKN80_03375"
     CDS             6723..7877
                     /locus_tag="PKN80_03375"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNQ35086.1"
                     /translation="MKRLKVTIRDSRVICKQPGRYIGWPTVGRLPDGELLAVFSGDRD
                     AHVDPFGKSFLVRSRDGGDTWSAPELINDTPLDDRDTGLCVCRDGTVLASWFTSHYCR
                     DSYLKYLAKYLGAAKAEPEERWRRKLASISPEDVRFWAEEMREGDYRVLGKWIRRSVD
                     GGRTWEDPVRVPVTTPHGPVELADGRLLFLGNSGSYDLNNGKMLAVESSDRGRTWTVI
                     GRMNMHPPYKNETPGGYAFLCEPHLVETAPGRLLALARYEDVLKVEPSCLWQSGSEDG
                     GRTWSEPYSTGILGKPPHLCRLSDGRVLATYGYRHPPYGQRACLSVDGGRTWDYADEI
                     ILRDDAPSGDLGYPASAELADGMIITVYYQQEPPGDEKPCLMATRWRFGE"
     gene            7909..8625
                     /locus_tag="PKN80_03380"
     CDS             7909..8625
                     /locus_tag="PKN80_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNQ35087.1"
                     /translation="MKTTELAKRIRRDLAAGGREAVRESTRRFFKEPIRCHGLKSAEV
                     QAVAKKYWPEVKGAARDEIFSFCEALYSSGFLEEASIASIYLPKFAAWFEAGDINIFQ
                     NWIERYIDNWAKCDMFCNHTVGDLVGKYPELTDVLKAWTRSGNRWLRRAGAVSLVVPA
                     KKGRFLKEVFEIADLLMADEDDLVRKGYGWLLKEASRLHRDEVFDYVVRNRWKMARTA
                     LRYAIELMPKELKSEAMKLP"
     gene            complement(9153..>9480)
                     /locus_tag="PKN80_03385"
     CDS             complement(9153..>9480)
                     /locus_tag="PKN80_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNQ35088.1"
                     /translation="PYWRQLPPTEDVAKGCLAHSISDFLKLPELIASFGRLGYDVVEM
                     VLANQDDWDRYEAPKWLTMRRWLEANPNDEFAKEVRAQLTSEPERHATYTREYLGWGV
                     FALMKR"
BASE COUNT         1573 a         2952 c         2990 g         1965 t
ORIGIN      
        1 gaggaagggc aggacacctc cgacacggac ggcgccgacg agctgctggt ggtatacgcc
       61 cccgaggcgc aggccttcgg gctggccagc ggcgtggctg ccaatgacgg cgacctcctg
      121 ctgcacttcg gggccaccac cgggtggcgc ctggacgaga actggggcga cgcgctggtg
      181 cgcttcctga ccagttggcc ggtgcgcctg ctgctggagg ggccgggcgg aaaacaggag
      241 tggcgccggg agaccggccc gggcggcgtc gtcgccgtcg agggcgtcaa ggtggataag
      301 ccgggcgtct actttgcccg ggcagccgcc gccggcgatg aaaaggtggc cggttcgagc
      361 aatccgatgc tctgcgcgga ttccggcgaa ggcgtcttct ggggcgactg ccattgccat
      421 accggctggt cggacgggat cggcaccgtc gaggacaatc tcctttttgc ccgcgaccgg
      481 gcttttcttg atgtcttcgg cttcgccgag cacctggcgg tgaaagactt cacgacccag
      541 ccggtggaca agggcggctc cgactgggcc tatcttggtc cgaccatggc cgaggcgagc
      601 aaccgctgga acgcggccgg caagttcgtc accatgctcg gctacgagta cacgcccggc
      661 aagacggtcc gggatccctc gggcgattgc tgcgtcttca gccggggcgg cagctggagc
      721 gacctgccgg cggccaccga gttgaatgac ctgctggcgc tcagcaaggc cgccgggtgc
      781 ctggtgatac cgcacgtggg cggccggacg ccgaagtggg acaaggtcgc ctttgctccg
      841 gagacgacgc cgctggtcga gatcgcctcg atgcacggca agttcgaggc ctttgcccag
      901 gccggtctcc agcagggtta caagcttggc ttctgcggca tgtccgacgg ccattacggg
      961 aagccgggct atgactgctg ggccctgcac ggccgtatcc ggtcgcttaa ggagcgtaac
     1021 ttctcggtcc agagcgccat caccgccttc ctctgccggg agctgacccg cgaggcggtc
     1081 ttcgagagcc tgcggcggcg ccggacttac gccaccaccg gcgagcggat cttcctgcgc
     1141 ttcgaggtca acggcgcgtc catgggagaa tcgctggtca ccagggagcg gcccgagatt
     1201 catatccggg tggccgggag cgcgccgatc agccggatcg acctgatccg gggcggccgg
     1261 cgggtggcga tgtgggagtt caagggagag aaagaggcgg agatacgctg gatcgacaag
     1321 gcgccctccc ctgccgagac ttattattac gcaagaataa cccaggccga cttcgccaac
     1381 gcctggtcga gcccggtctg ggtggcctat accggtccgg atccggccgg cccggagcag
     1441 gagagccaac tgcccgaatg gaacgccgcc ccggagtggc cggcgtcgac cggcctggag
     1501 gtgccccggg atttgctggc ccttcagctg gaggttttcg aaaggaaggg aatcgccgac
     1561 cgtttccccg acctggagcc ggtcggaata ttttctgaga accgggggcg cttcgtcttc
     1621 taccgcggct acgaccgtga gaccggttcc cggatccact gccagcacta ttttgagttt
     1681 gacgacgacc ggctgctcat ctcgccgggt tggtcgccct acggccaggt gctgtattag
     1741 gtttggtaat accctgaatt ggaaaaatta aaattcgcat tttacttcgg gttgaataaa
     1801 agatcagatt ttttgactga cggtgtgaac tcgaattttg ttgctatttc aggtttgacg
     1861 ccgggctgaa aaaaccctaa aatggaggca tgataaagaa ggtcgggttc tttctgcgtt
     1921 ccggggtccg gctctccgtt gccaatcttc tcctcgcttt attggtgctg cttttgtgtt
     1981 tttcaaccat tgtgatcatc ttccgcagtg aaattctgca attgaaacgg gataaaaagg
     2041 ttttgcatga aaaggtcttg aatttaatcg aaacgggcac cgcatcgccc gatgttatta
     2101 aaagatggat atctcgtccg cctcagcttt cctcgaagga aattcagtat ctgaaaaaaa
     2161 ggggtttgaa ggatccggtc aacgacatag ccgaagatct catgaagcac aatgaattta
     2221 taccctaccg gggtatcgtg ggtggcaaaa tgcagttttt tgaggtgtac gtaatttcga
     2281 ctcgcctggt tcgggcctac tttgaagatg gacatattgg tggttggatg atgcttgaat
     2341 atcgggtcaa caagggcggc cgaatttcct gggaggtatt agagtcgtac cttaactgac
     2401 gcggaattcg agggagagag tcagccgttt ttgatgaatg ttggtgaaaa acgcagagca
     2461 gaagcaggcc gcttctaaaa acaaaagggt tggaagatga tcagccgggt gctttccgaa
     2521 gaacaggttg aaaagatcca ccgggcctcc ctggccgtcc tggaacgggt cggagtcgcc
     2581 gtgccgcacc ccgaggtcct gggccggttt catgattcgg gggcggcggt cgatgaaaaa
     2641 acgcaaagag tgaagatccc tcccgatctc gtgatgcgcc tgttgaagca ggccggcaag
     2701 gagtttacca tctacggacg ggacctctcc cggaaggccg ggttcggtaa gggcacgcgg
     2761 aattacaacg ccagcggcgg ccaggcctcc tgggttgaag agcccggccg ggaacggcgt
     2821 tacgcgaccc tgaccgacgt tgcgaccgcc gcccgtttct gcgacgggct ggagcggatc
     2881 aacatcgccg gcgccatggc cgatccccac gagatcccgg tccattaccg gtgcgtggag
     2941 gtggccgccg ccatgcttga aaataccacc aagcccctta ccttctggtt ttatgaccgt
     3001 ccttccgcaa ggcacatcgt ggagatgctc atcctcctgc ggggaagcgc cgaaaaagcg
     3061 gcggaattcc cgctctgcta tcccttcctg gagcccatca gcccgctctc ctttcccttc
     3121 cacggcatcg acctgatttt cgagacggcc cggctcaacc tgccggtacc ggtagggccg
     3181 atggcccaga ccggcctctc ggctcccggc accctggccg ccaccatggt ccaggagaac
     3241 gcggagatcc tggccggcat ctgcatcacc cagctgatcc ggccgggcct gccggtctgc
     3301 tacggcggca tctgccacgt ctttgacatg gccaccaccc agatggtctt cggcggaccg
     3361 gagcaggcga tcttcggagt cgggatgacc cagctgggca agcattacgg cctgccggtc
     3421 tacatcaacg tcggcctgac cgacgccaag tcggttgacg cccaggccgg cctggaggcg
     3481 ggcgtcaccc tggccctggg cgccgcggcc ggggcggata tcttcggcca cctgggcatc
     3541 tgcggggttg accagggcgc ctccctggat ctgctggtct tccaggcaga gatgatctcc
     3601 tacgtcgagc acctgctgcg cgatattgat ttcagcgacg aggcgctggg gatcgatgag
     3661 atcgagacgg tcgggcccgg cgggatttac atcgaccggg aattcacggc cggccgtttt
     3721 cgaaaggaac tctggttccc gaaactcctg gaccgccggt attaccaggc ctggctggag
     3781 tccggcgccc ggggcgcggc ggagaactgc cggaggcgca aggaggagat actgcggagc
     3841 cacgcccccg agccgctcgc cccggaattg aagctcggcc tcgcggaaat cgtcgcggcg
     3901 gcgaagaagg atctgaccgg gtaagccggt gctccgggcg ccgtcactat tcgacgccgg
     3961 ccgttaattc aaaggccggc gggagggaaa atgtttgtta tcgcggtgat cgttatcctg
     4021 cttctgttga tctttctgcc cggccgaagg acttcccctg agccgcccca acgccccgct
     4081 tcccaggcgg atgaactgaa gtttttgctc cgccgcgtcg ccgatctgga acggcgcctc
     4141 gaatcgcttg agggaaaagc cccgccggcc ggcccggccg cggagacgcc cggccgggag
     4201 gagccggccg cggccgttgc aacgccgccc tccggcccgg agacggcgat accgctggcc
     4261 gcccgcatgg ggccggcggc cgcgcgcacg gttggcacgg ccaggccgga ctccggccag
     4321 gtgcgccgcc ggctcctttc gctcgaaaga acgatcggcg aaaagtggct ggcctgggtc
     4381 ggctcgctgg tcttcgccgc cggcttcggc ctcctgctca agtacgcctt cgagcagggc
     4441 tggctcaacg agtgggcccg gaccggcctg ggctttgcgg ccgggctctt tttcctcttg
     4501 ctgagcgaac tcctttaccg taagagttac cgggtcctgg cccagggggt ggcgggcgtc
     4561 ggaagcatca tcctctacct gaccgtcttc atcgcccacc gtttctacct ctttttcgac
     4621 accccggagg cctccctgct tttcgtggcg gtttcgataa ccggcatggc gctggccgtt
     4681 cttcacgatt cctacgcggc cgccctgctc accgtcctgg gcgccttcgc tacgcccgtc
     4741 ctgctcttca gctcgatctc ctacctttat tactacggcc cggccttctt ctcctacctg
     4801 ctcttcatcg acctgggggt gctttacgtt tcggtagtcc ggcgctggcg ggcccttttc
     4861 ctgctggcct tcttcggcac gaccggctac ctcggcctct ggtacctgca gaagtatacc
     4921 ggcgccgatt tccgggtcgc gatactcttt tctattcttt acttcatcct cttctcattt
     4981 acggctacgc tttacagtct ttggcgccgc ggccggtcct tccgcgagga cctggctttc
     5041 gtaatcctga acccggtctt cttcttcatc ttctggtatg gattgctctc ccggcagggg
     5101 ctggattggc tgctgcccct ggttccgctg gccatggccg ccttccacta cctgctggct
     5161 cggatcgtcc ggctgcggaa ccccgaggac cggcttctct actttgccct ggccggcacc
     5221 gccaccggcc tgctgaccct gccggtgccc atctggatca aggacagctg gatgacgccg
     5281 gcctggggcg tcgaggcggt catcctggtg gcggtcggtt cctttttcaa ccgccggttc
     5341 ctgcggatcg gcggcctggc gatcctctgc ctggttggct accggctggc cttgatcgac
     5401 ccgttcgccg cgcgctcggc cgggtttacg gctggtttta tagcccgccc ctttttaaac
     5461 ctgcgtttcc tgccccagct gatcgccgcc gcctgcttcg gcctggcggc cttcgccttc
     5521 aagaacctgc cgggactgcc ggacggggag cggaagtttt gcggacggct gtggctggcc
     5581 ttcgccgttt ttcttttctg gatcgccaac ctcgagttgt tcggatactt ttcgcgcctg
     5641 ggcgcttttg ccgaaagcat gaagtggccc ttcagcacca tcctctggtc cctcttcgcc
     5701 tggtggttat tcttcgccgg gatgctgcgc cggaacggac gcctcttcaa ggccggcctc
     5761 ggactggcgg ccgcgaccgt cctgaaggtc atgctgcttg acacgccgct gcttcgcggc
     5821 caggggcacg gctatccttt cctgctgaac ctgcgcttcc tcgctcccgc ctttttactg
     5881 ggcgcgctgg ccaccgccgc cttctttcac gcccggctcg gggatggcga aaggcccgga
     5941 ggccgccggg cccttccctg gctctggact ttcttcaccg ccctgctctt ctgggttttg
     6001 aatgccgagg tctttgcctt cttcggccag gtggaactca acgcctttcc ggagagcttg
     6061 atcttcaccg acattctctg ggcggtcttc gccctggtcc tggtgcggca gggcctccgg
     6121 aacggagtat cggccctccg gacggccggc ctcctgctgc tggtagccgc tttcgtcaag
     6181 gcaatcttgg ccgacctctt cgacgggtat ggtctcttcc tttccggcgg ggatcccctg
     6241 ctcttcaacc tgaagtttct gggcgcggcg gccatgctcg tggtggccgc ccacctggcc
     6301 tccctctacg ccgaaccggg cgaaggccgg acttatttcg agcggccggc cgtaccctgc
     6361 ctctggatcc tcttcctggt cctgctcttc gtcgagctca acctgcaatc gaggctgctc
     6421 ctgcagggaa tctgggacct cgaaaggcag cggttcgccc tggtcctctc gctgctctgg
     6481 ttcctttacg ggttcggcct cctgctggcc ggcatcgtga aggagtcgct gcccctgcgc
     6541 ttttcggccc ttaccctctt cggagtgacg cttttgaagg tattcttcgt tgacctgaac
     6601 tttatcggca aggcctaccg gacggcggcc ctgctggcga tcgggagcct gctgctgatc
     6661 ggcgcctacc tttaccgccg ctaccggcag cggatttcgg aacgggagta gcgaaaggag
     6721 ccatgaaacg gttgaaagtt accatccggg attcgcgcgt catctgtaaa cagccgggtc
     6781 gctacatcgg ctggccgacc gtcggccgcc ttcccgacgg cgagttgctg gcggtctttt
     6841 ccggagaccg ggacgcccac gtggacccct tcggcaagtc cttcctggtc cgctcccggg
     6901 acggcggcga tacctggagc gcaccggaac tgatcaacga cacgcccctg gacgaccggg
     6961 ataccggact ctgcgtctgc cgcgacggca cggtgctcgc gagctggttc acttcccact
     7021 attgccggga ctcttatctg aagtacctgg cgaagtacct gggtgcggcg aaggccgaac
     7081 cggaggagcg ctggcggcgc aaactggctt cgatcagccc ggaggatgtc cggttttggg
     7141 cggaagagat gcgggaaggc gattaccggg tcctcgggaa atggatccgc cgctcggttg
     7201 acggcggccg cacctgggag gacccggtcc gggtgccggt taccacgccg catgggccgg
     7261 tcgaactggc cgacggccgg ctgctcttcc tcggcaacag cggcagctat gacctgaata
     7321 atggaaagat gctggccgtt gaatcctccg accggggccg cacctggacg gtgatcggcc
     7381 ggatgaatat gcatccccct tacaaaaacg aaacgccggg cggctacgcc ttcctctgcg
     7441 agccgcacct ggtcgagacc gcgccgggcc ggctgctggc cctggcccgc tacgaggatg
     7501 tgttgaaggt ggagccctcc tgtctctggc agtccggctc cgaggatggc ggccggacct
     7561 ggagcgagcc ctattcgacc ggcatcctgg ggaagccgcc ccatctctgc cgcctgtccg
     7621 acggccgggt gctggcgacc tacggctacc ggcatccgcc ctacgggcaa cgggcctgcc
     7681 tttcggttga cggcggccgc acctgggact acgctgacga gatcatcctg cgcgatgacg
     7741 cgcccagcgg cgacctgggc tacccggcct cggccgaact ggcggacggc atgatcatca
     7801 ccgtttacta ccagcaggag ccgccgggtg atgagaagcc ctgcctgatg gccacccgct
     7861 ggcgattcgg ggagtgacgg gatcgctgta aagttcaata ctaaaataat gaaaacgacc
     7921 gaactcgcaa agcggataag gcgcgacctg gccgccggcg gccgcgaggc ggtgcgggaa
     7981 tcaacccgcc gtttcttcaa ggagccgatc cggtgtcacg gcctgaagag cgccgaggtc
     8041 caggccgtcg ccaaaaaata ctggccggag gtcaagggcg ccgcccggga tgagattttc
     8101 tccttctgcg aggcgctcta ttcttccggg ttcctggagg aggcctcgat cgcctcgatc
     8161 tacctgccga aattcgccgc ctggttcgag gccggcgaca tcaacatttt tcagaactgg
     8221 atcgaacgct acattgacaa ttgggccaag tgcgatatgt tctgcaatca caccgtgggc
     8281 gacctggtcg ggaagtaccc ggaattgacg gacgttctga aggcctggac ccggtccggc
     8341 aaccgctggc tgcgacgggc cggcgccgtc tccctggtcg taccggctaa aaaaggccgg
     8401 tttttgaagg aggtttttga gatcgccgac ctgctgatgg cagacgagga tgacctggtt
     8461 cggaaaggat acggctggtt gttgaaggag gccagccggc tgcaccggga tgaggttttc
     8521 gattacgtgg tcaggaaccg gtggaagatg gcgcgcaccg cgcttcgcta cgccatcgag
     8581 ctgatgccga aggagttgaa gtccgaggcg atgaaactgc cgtaggaaag gctggatttg
     8641 cccgtcatcc ccgcctgccg tccgcctgcg cgtgaattac gcgcttaggc gcgattcgtc
     8701 tgaaaaccca tccaaaaccc tgaagtgtga accttgtttt tcaggttctc tacttcaggg
     8761 gtcattcccg cggattgttg gcgggaatcc atgtttttat ccgttacttt taatttaaaa
     8821 ccttttaatg attggatacc cgcctaccac atgcgggtat gacgggcgtt ttggataccc
     8881 gcctgcgctc agaaaagaag cttcggcggg caagcctgcc tgcggcgtgc gggtatgacg
     8941 ggcttttgtt cctggtggtc gagttgtttc ggggggaatg ccttgcgcgg ccgacatcgc
     9001 cgctgcgatt ccgcaggctt cacccatcac cttagcctgt ttcgggcctg ggctcttgaa
     9061 cgtgcggagc ctttggatag agatttcggc ggggcgagcc gccacctggc gcgcggcccg
     9121 gccgttgaca atcggccggg taccgcaacc cctcaccgct tcatcagcgc gaatacaccc
     9181 caacccaggt attcccgtgt gtaagtggcg tggcgttcgg gttccgaggt cagttgggct
     9241 cgaacttctt tcgcgaattc gtcgttagga ttggcttcaa gccatcggcg catggtgagc
     9301 catttaggcg cctcgtatct atcccagtca tcctggttgg ccagcaccat ttccacgacg
     9361 tcatagccaa ggcggccgaa agacgcgatc agttccggaa gtttgagaaa gtctgagatt
     9421 gagtgggcaa gacacccctt ggcaacatct tccgtcggcg gtaactgccg ccagtagggc
//
LOCUS       DAOQYN010000054         9422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_7777_length_9422_cov_5.072382, whole genome shotgun sequence.
ACCESSION   DAOQYN010000054 DAOQYN010000000
VERSION     DAOQYN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9422
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7777_length_9422_cov_5.072382"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PKN80_03390"
     CDS             <1..706
                     /locus_tag="PKN80_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNQ35089.1"
                     /translation="GSRTLKDAMNEAMRDWVATVETTHYLIGSVAGPHPFPTMVRDFQ
                     AVLGREARQQVIKRERRLPDALVACVGGGSNSMGLFHPFVRDRSVAMYGVEAAGAGIA
                     SGRHAATLSAGRPGVLHGSYSYLLQDGDGQVQIAHSVAAGLDYPGVGPEHSHFKETGR
                     VTYAAVTDRQALEAFHLLSETEGIIPAVESAHALAYLVKNRKDFAGKVVVMCLSGRGD
                     KDLDEVRRVEAAKEPS"
     gene            703..1530
                     /gene="trpA"
                     /locus_tag="PKN80_03395"
     CDS             703..1530
                     /gene="trpA"
                     /locus_tag="PKN80_03395"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNQ35090.1"
                     /translation="MNRIERKFRELERAGRKGLILYLTAGYPSLEATRALVPGLEKAG
                     ADIIELGVPFSDPIADGVTIQRTNAAALAAGVNLVRILKLVAEIRRESQVPIILMGYY
                     NPILAYGLEKFVRDSWKAGVDGLIVPDLPPEEAAPLRRELKKARLGLIFLLAPTSDDE
                     RIALVAGRSEPFIYYVSVAGVTGARASLASEEIGAQVARIRRQSRRPVCLGFGINGPE
                     ALRKLAPLADGFIVGSALLNCIEKAGTRYQAAAEEFVAGMAGVLKETAGPGPGAKKN"
     gene            1587..2555
                     /locus_tag="PKN80_03400"
     CDS             1587..2555
                     /locus_tag="PKN80_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35091.1"
                     /translation="MKKIIAALLILTFGWLGRPAAAEKEAAAFYPVQPAESRLAAEVG
                     DYLARAFGRYYQVLPPEQFEPVLKAAQARNPEVPLLNLYPSLGRDLKVRYVVSGRVSG
                     DTQLEQVEVLVLEVATKRGRIFRTSGAAAGRRAALARLGDDLREYLVRRRKLGLESER
                     ESDIVQAVGRTLMYRGPRPSVSVAIEETGLPEGMQQLWAEKNFGYYLQQLGFTWHLGE
                     GELLRSYARRRWAGSGQALPLTLASHFFVIGRAEALETIIHETGLVGSRAKLEVEVLD
                     RLARVVDRRLIEVEAYGLSSGAAAVNALNIAASEAAIEILPLRRHP"
     gene            complement(2552..3349)
                     /locus_tag="PKN80_03405"
     CDS             complement(2552..3349)
                     /locus_tag="PKN80_03405"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HNQ35092.1"
                     /translation="MLDDTIISRAIIETYYREFLDHLETDAAIVGAGPAGLTCAYYLA
                     GRGLKVTLYEKLLRTGGGMPGGGMMFNQIVVQAEALPILKEFGIRFREYRDGYYTADS
                     LEATARLTAGAIQAGAKIFNLIGAEDLYLREGRVCGLVLNWGAVRSAGLHVDPLTIRS
                     RAVVDTTGHEAVLCRLLAEKNGGGLRTASGGVVGEGPMWADKGERLIMANTGEIFPGL
                     YIAGMTVNAVCGGPRMGPIFGGMLLSGRRCAELIGRDLAGKGKRKKR"
     gene            3778..5229
                     /locus_tag="PKN80_03410"
     CDS             3778..5229
                     /locus_tag="PKN80_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35093.1"
                     /translation="MAQISLNGTELAGLPNDLALWYQAIPPVSRPGEVIDLMVKMRRP
                     TRKPIRIEARLNNRERVSAVIPPSPPRLQFNFIGFSRDYDRGYLYLENAGPEDLSIER
                     ISCDGQEITPRCFIPDRTLRPSSKGLVIYTPPARLSQGKYLVFKAIAGGETAAASVRV
                     YSHFPITTFGREDTRSEFGFDPAGFEMKYPASEAARSQPPFKAYHLIDDPACADGKAY
                     QIIGTTAREIVKRAAECWTVDPVHPNLIYSCEHYKPDSYFIYAETTDIMVIDPYAMYF
                     NRNQPRQDAYFTSIARRASAPRLLWTIPQAFTAGEKRFPTPEEERIIVWSEVGEGSKG
                     IWYYRYNPRGGYPASPPLEEEIGKINRELRLVRDYILISEPFPLAAAEAAKVTAYSLL
                     CGDRAILLILVNNDTESRFEKEETPFTYGPKENFKVRVSIPDWLRVKEVQELSPEGLK
                     PAPVRHSGEGNNLTFSIDRLDISREFLILVTRK"
     gene            5331..6608
                     /locus_tag="PKN80_03415"
     CDS             5331..6608
                     /locus_tag="PKN80_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35094.1"
                     /translation="MIKETAESLLFQTDKSQITIPRERIKEIIIHTPFFYAEKAFEAE
                     NYAEALRLYREYLKKYPTGEESEPAAFKTGLCYLKMADPEPALVAFEAFLKTYPDSAF
                     STEAALHIGEAYFAGNRKEKAGEYFQKALASASKSVAGSAAYFLFRLWEDKSPAETLE
                     FCENYLKEHPRSERTPAVIYLKAEVLYSRAVQRSQADQNISDYQAITEMLEETAGEHP
                     GASMPENILKLQMDCYERQARYAQKHKTLKKYADLVSRGDKEKAARMIREAADKLITE
                     GEVTEAIILYRLIKAEYPETALAPEVCFQIAKILQEEANKHGAIAAEYVKECETLIQQ
                     YPQTQFAEEACFALAKKYQNTGKYAQAIEHLNYFLKNYPESKYIESGVFLLGFLYRQT
                     KQNDEARKIWEYYLKKFPDDNIYLEIIKTYLRE"
     gene            7140..8063
                     /locus_tag="PKN80_03420"
     CDS             7140..8063
                     /locus_tag="PKN80_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35095.1"
                     /translation="MKKTIFVIGFAATFITMASFAFGANAEEICMKQYKYFQKLYSIQ
                     FDSKVCVTLSKDILLEQPNLSNQLIWEIKFISNGSKYRSEVTSIDPIKRLKKTFITTY
                     NGEDYRILEIGEKSILYLKKSWNTDNPYNGTITILTPFQFAFQKGDIGTIETLKKTEI
                     WQRLANKAIEVKSFQKLGYKGINITFKVSDFNNAKERIYEVFFAEDLDYYPIFWRTID
                     PTTKRCDEISVLEMRIYNIDNDRIFIPIRITGEEKFNNGGIAQTGSEEINPQTVKINQ
                     PVNNGIFTLSVFQANAVHDSDTGIWFYPNQR"
     gene            8115..9191
                     /locus_tag="PKN80_03425"
     CDS             8115..9191
                     /locus_tag="PKN80_03425"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNQ35096.1"
                     /translation="MRVTNRKLQKIFHVAIVLAVFLIGLNVGLFFPKVSNYLRQNPPQ
                     IIITEPKRDFGCVYPGVTLKHDFTIINGGKRSLYIKRIRTNCTCTQARIDNNVVPPKR
                     KTKLRIATVASKYKTTINDFVALHSNDPITPIKMVYITGQVTPNIEASPSVVNFGIVE
                     PWELPLTQKIVIYREPYGKATHDDLKIVEDSGRLFTNLTKLDEKRWELKVTLPKNHPF
                     GFIIGKIWIENNSSENFKIEIPIMGKIISSVVIIPDEIFYGVIDPKKEIFQKIRIGGG
                     KEIVTDVHVISKSPFLDGMLDVKIKKIAGDFQLFTTLNSHKLKQTKDLIEGCIYIKVK
                     TKEGKEYTIGIPVLGIINSTVVNH"
BASE COUNT         2238 a         2525 c         2658 g         2001 t
ORIGIN      
        1 cgggagccgg actctgaagg atgccatgaa cgaggcgatg cgcgactggg tcgccacggt
       61 cgagacgacc cattacctga tcggctcggt ggccgggccg catcccttcc cgaccatggt
      121 ccgcgatttc caggcggtgc tgggccggga ggcccgccag caggtgatca agcgggagcg
      181 ccggctgccg gacgctctgg tcgcctgcgt cggcggcggc agcaacagta tggggctctt
      241 tcatcctttc gtgcgggacc ggtcggtggc catgtacggg gtggaggcgg ccggcgcggg
      301 catcgccagc ggccgccacg cggccaccct gagcgccggt cggccgggcg tcctgcacgg
      361 cagttacagc tacctgctcc aggatggcga cggccaggtc cagatcgccc actcggtggc
      421 cgccggcctc gattatcccg gggtgggtcc cgagcacagc catttcaagg agaccggccg
      481 ggtgacctac gcggccgtga ccgaccgcca ggcgctcgag gccttccacc ttctctccga
      541 gaccgagggg atcattccgg ccgtcgagtc ggcccacgcc ctggcctacc tggtgaaaaa
      601 ccgcaaggac ttcgccggca aggtggtcgt catgtgcctt tccggccggg gcgacaagga
      661 cctcgatgaa gtccggcggg tcgaggcggc gaaggagccc tcatgaaccg gatcgagcgg
      721 aagttccggg agctggagcg cgctggccgc aagggcctga tactctacct gacggccggc
      781 tatccctcgc tggaggcaac ccgggcgctg gtgcccggcc tggagaaggc cggcgccgat
      841 atcatcgagc tgggcgtgcc cttctccgac ccgatcgccg acggcgtgac cattcagcgg
      901 accaacgccg ccgccctggc cgccggggtc aacctggtcc ggatcctcaa gctggtggcc
      961 gagatccggc gggaatccca ggtgccgatc atcctgatgg gctattacaa tccgatcctg
     1021 gcctacgggc tcgagaagtt cgtccgcgac agctggaagg ccggcgtcga cggcctgatc
     1081 gtgcccgacc tgccgcccga ggaggcggct ccgctgcgcc gggagctgaa aaaggcccgg
     1141 ctgggattga ttttcctgct ggcgccgacc agcgatgatg agcgcatcgc cctggtcgcc
     1201 ggccgcagcg aacccttcat ctactatgtt tcggtggccg gagtgaccgg agcccgcgct
     1261 tccctggcgt ccgaagagat cggcgcccag gtcgcccgca tccgccggca gagccgccgg
     1321 ccggtctgcc tgggtttcgg catcaacggc ccggaggcgc tccggaagct ggccccgctg
     1381 gccgacgggt tcatcgtggg cagcgccctg ctgaattgca tcgagaaggc cggcacccgc
     1441 taccaggcgg ccgccgaaga gttcgtggcc gggatggccg gggtcctgaa ggagacggcc
     1501 ggcccgggtc cgggagctaa gaagaactga cccgcggccc cgccgggccc ggttttccac
     1561 gtgaccgatt ctggagagag gcgaagatga aaaaaataat cgcggcgctg ttgatcctga
     1621 ccttcggctg gctcggccgg ccggcggccg ccgagaagga agcggccgcc ttctacccgg
     1681 tccagccggc cgagagccgc ctggccgccg aggtgggcga ttacctggcg agggccttcg
     1741 gccgctacta ccaggtcctg ccgccggagc agttcgagcc ggtgctcaag gcggcccagg
     1801 cccggaatcc ggaagtgccg ctgctcaatc tctacccttc cctgggccgg gacctgaagg
     1861 tgcgctacgt ggtgagcggc cgggtgagcg gggatacaca gctggagcag gtcgaggtgc
     1921 tggtcctgga ggtggccacc aagcgcggcc gcatcttccg gacctcgggg gcggccgccg
     1981 gccgccgggc cgccctggcc cgcctgggcg acgatctgcg cgagtacctt gtccggcgcc
     2041 ggaagctggg gctggaatcg gagcgggaga gcgatatcgt ccaggcggtc ggccgcacgc
     2101 tgatgtaccg ggggccgcgc ccctcggttt cagtggccat tgaggagacc ggcctgccgg
     2161 agggcatgca gcagctgtgg gccgagaaga acttcggtta ttacctgcag cagctgggct
     2221 tcacctggca cctgggcgag ggcgagctgc tgcgcagtta cgcccggcgc cgctgggccg
     2281 ggtccggcca ggcgctgccg ctgaccctgg ccagccattt cttcgtgatc gggcgggccg
     2341 aggccctgga gacgatcatc cacgagaccg gcctggtcgg aagccgggcg aagctggagg
     2401 tcgaggtgct cgaccgcctg gcccgggtgg tcgaccggcg cctgatcgag gtcgaggcct
     2461 acgggctcag ttcgggggcg gcggcggtca acgcgctgaa catcgccgcc tccgaggcgg
     2521 cgatcgaaat cctgccgctc cggcgtcacc cttagcgttt tttccgtttt ccttttccgg
     2581 ccaggtcgcg gccgatcagc tcggcgcacc ggcggccgga gaggagcatc ccgccgaaga
     2641 tcgggcccat ccggggcccc ccgcagaccg cattgacggt catcccggcg atgtagaggc
     2701 cggggaatat ctcgccggtg ttggccatga tcagccgctc gcccttgtcg gcccacatcg
     2761 gaccctcgcc caccacgccg ccggaggcag tgcgcaggcc gccgccgttc ttctcggcca
     2821 ggaggcggca gagcaccgcc tcgtggccgg tggtgtccac gaccgcccgg ctccggatgg
     2881 tgagcgggtc gacgtggagc ccggcgctgc ggaccgcgcc ccagttgagg accagcccgc
     2941 agacccgccc ttcccgcagg taaaggtcct cggcgccgat caggttgaag atcttggcgc
     3001 cggcctggat ggcgccggcg gtcagccggg cggtcgcctc cagggaatcg gcggtgtagt
     3061 agccgtcccg gtactcgcgg aagcggatgc cgaactcctt caggatgggc agggcctcgg
     3121 cctggacgac gatctggttg aacatcatgc cgccgccggg catgccgccg ccggtccgga
     3181 gcagtttttc gtagagcgtg accttcaggc cgcggccggc caggtagtag gcgcaggtga
     3241 ggccggccgg gccggcgccg acgatggccg cgtcggtctc caggtgatcg aggaattccc
     3301 ggtagtaggt ttcgatgatc gcccgcgaga taatcgtgtc gtcaagcatt ttttctcctt
     3361 ggcgggggag ttaaagggat ccgtccgggc ggaatccctc ggaggaaaac ttcgataaat
     3421 attttaccat gcccgggcgg cgatacatgg atttgacaaa aggtctgggg gtgggtatta
     3481 taggggcaaa cgcgcggccg aaaggatttt ttccggcgcg ccccaaaatt cgaaccggga
     3541 gggaatatgc tccgcggatt ttggcgacga attttttcct ggcctgcctt ccgccacccc
     3601 cgggccctct ctttaaccct gccccttttc ctggtatccg cgccaggcca cggccagccg
     3661 gcctatttgg aatccgggat tgcctttata gaccacgcct ttttccggaa ggttgatcct
     3721 ctcgatccgg gcactgtcca cctgttcatc aagaattccg gcgccgcccc cctttccgtg
     3781 gcgcagatca gcctgaacgg aacagaactg gccggcctgc ccaatgacct ggccctctgg
     3841 taccaggcga ttcccccggt cagccggccg ggtgaggtta ttgacctcat ggtgaagatg
     3901 cgccggccga cccggaaacc catccggatt gaggcccggc tgaacaaccg ggagcgcgtc
     3961 tcggcagtca tccccccctc tccgccgcgg ctgcaattca acttcatcgg tttcagccgg
     4021 gattacgata gaggctacct ttatctcgag aacgccggcc cggaggattt aagcatagaa
     4081 agaatctcct gcgacggcca ggagatcacc ccgcggtgct tcatcccgga ccggaccctg
     4141 cgtccttctt ccaaagggct ggttatctac acgccgccag cccgcctctc ccagggcaaa
     4201 tacctggttt tcaaggccat cgccggcggg gaaaccgccg ccgcgtcggt ccgggtctat
     4261 tcgcactttc ccatcaccac tttcggccgg gaggataccc gctccgagtt cggctttgac
     4321 cccgccggtt tcgaaatgaa gtacccggct tccgaggcgg cccggagcca gccgcccttc
     4381 aaggcctatc acctgattga cgatccggcc tgcgccgatg gcaaagccta tcaaatcata
     4441 ggcaccaccg ccagggagat tgtcaagagg gccgcggaat gctggaccgt tgatccggtc
     4501 caccccaacc ttatctactc ctgcgagcat tacaagcccg actcctattt catctacgcc
     4561 gagacgaccg atattatggt cattgatccc tacgcgatgt attttaatcg gaaccagccg
     4621 aggcaagacg cctatttcac ctccatcgcc aggcgggcca gcgccccgcg cctgttgtgg
     4681 accattcccc aggccttcac ggccggcgag aaacgctttc cgactccgga agaggagcgg
     4741 atcatcgtct ggtcggaggt gggtgaaggc agcaagggga tctggtatta ccggtataat
     4801 ccgcgcggcg ggtatccggc cagcccgccg ctggaagaag agatcgggaa gatcaaccgg
     4861 gagctccggc tggtccgcga ttatatcctt atcagcgaac cctttcccct ggcggcggcc
     4921 gaggcggcga aggtaaccgc ctactccctt ctctgcgggg acagggcgat tctgctgatc
     4981 ctggtcaaca acgataccga gagccggttt gagaaggagg agacgccctt cacctatggg
     5041 ccgaaagaga acttcaaggt ccgggtgtcc attcccgact ggctgcgggt aaaggaggtc
     5101 caggaattaa gccccgaggg actgaagccg gccccggtcc ggcactccgg ggaagggaac
     5161 aacctgacgt tttccataga ccggcttgat atctcgaggg agttcctgat cctggtgacg
     5221 cgaaaatgaa gcggcttctc ctgcttgttt tcggcttctg cctgctgtgg accggttccg
     5281 gcgccgccga ccaggtcatc ctgaagaccg gcaccgtctg ggaggggaag gtgatcaagg
     5341 agaccgccga gagcctcctc ttccagaccg acaaaagcca gatcaccatc ccgagggagc
     5401 gcatcaagga gatcatcatc cacacgccct tcttctacgc cgagaaggcc ttcgaggcgg
     5461 agaactacgc cgaggccctc cggctctacc gggagtactt gaagaagtat ccgacgggag
     5521 aggaaagcga gccggcggcc ttcaagaccg gcctatgtta tttgaagatg gccgacccgg
     5581 agccggccct ggttgccttc gaggcgttcc tgaaaaccta tcccgacagc gccttctcca
     5641 ccgaagcggc cctgcatatc ggagaggcgt attttgccgg gaaccggaag gagaaggccg
     5701 gagagtattt tcagaaggcg ctggcctcag cctccaagag tgtggctgga agcgccgctt
     5761 actttctatt tcgcctgtgg gaggataaat cgccggccga gaccctggag ttctgcgaga
     5821 attacctgaa agagcatccg cggagcgaaa ggaccccggc ggtcatctac ctgaaagcgg
     5881 aggtgctgta cagccgggcg gtccagcgga gccaggcgga ccagaacatc tccgactatc
     5941 aagcgataac cgagatgctc gaggagaccg ccggcgagca tcccggggcc tcgatgccgg
     6001 aaaacattct aaaattgcag atggattgct acgagaggca ggccaggtac gcccagaaac
     6061 acaaaacgct taaaaaatac gccgacctgg tttcacgggg agacaaggaa aaggcggccc
     6121 ggatgatcag ggaggcggcg gataaattaa tcacggaggg ggaggtaacc gaagccatta
     6181 ttctttaccg gctgataaaa gcagagtatc cggagacggc cctcgccccg gaggtctgtt
     6241 tccagatagc gaaaatacta caggaagagg ctaataaaca tggagcgata gcagcagaat
     6301 atgttaaaga atgtgaaacc ttaattcaac aataccctca aactcaattt gcagaggaag
     6361 cttgttttgc tttagcgaag aagtaccaaa atacagggaa atatgctcaa gcgatagaac
     6421 atttaaacta ctttctaaaa aattatccgg aaagcaaata catcgagagc ggcgttttcc
     6481 tcctcggttt tttatatcgc caaactaaac aaaatgacga ggcaagaaaa atatgggagt
     6541 attatttaaa gaaattccct gacgacaaca tctacttgga gattataaaa acatatttaa
     6601 gagaataagg aaaaggtttg taaaacactt ccattgatat tggtgtatgt ataagtttca
     6661 tctttataag attgcagtga attattgtat tgagaattta aggtccgaaa agcattatta
     6721 acatagggag gaataattat ggcaaaagtt aaattggtta ttactattac aagcttgaaa
     6781 atgtttctca tatctatttt ttgtgttttg gtattattat tttcgatttc caaagttttt
     6841 tctgtatcat gttacaacgt tggtagttgc ttacaagatt atatttgtga tgcgaatggt
     6901 atgtgtaaag cagtgggcga ttactcgcaa caaagatgcc ttacattgtg tggatatggt
     6961 catcactcca atgaacttgg cggttcctgt gggatttcat ttcccaaaac aattttttct
     7021 ttgaattgtc aatacccagg cctcactgga tgcggaggac taaggtgcac tcctgatgat
     7081 gaaaattgtc cgtaaattaa ctcagcgata cataattaaa aaaggtgagg aggatacaaa
     7141 tgaaaaaaac aatatttgtc attggttttg cagctacttt tattactatg gcttcttttg
     7201 cttttggcgc taacgccgaa gaaatatgca tgaaacagta taaatacttc caaaaattgt
     7261 attctataca gttcgattca aaagtatgtg tgacattgag taaggatatt ctgttagaac
     7321 aaccaaattt atcaaatcaa ttaatttggg agataaaatt tatatcaaat ggttctaaat
     7381 ataggtcaga agtgacttct atagatccaa ttaaaagatt aaaaaaaaca tttataacga
     7441 catacaatgg cgaagattat cgaatattag aaataggtga aaaatcaata ctatatttaa
     7501 aaaaatcttg gaatacagac aatccatata acggtacgat tacgatactg accccatttc
     7561 aatttgcttt tcaaaaaggg gatattggga ctattgaaac attaaaaaaa accgaaatct
     7621 ggcaacgcct ggcaaacaag gcgatcgagg ttaaatcttt tcagaaattg gggtacaaag
     7681 ggattaatat aacatttaaa gtatctgatt ttaataatgc gaaagaaaga atctacgagg
     7741 ttttttttgc ggaagacttg gattattacc ctattttttg gagaactata gatcccacaa
     7801 cgaaacgttg tgatgaaata agcgttttgg aaatgaggat atacaatatt gataatgacc
     7861 gtatttttat cccaatcaga attacagggg aagaaaaatt caacaatggt ggtatcgctc
     7921 aaacaggaag tgaggaaatt aatcctcaaa ccgtaaagat taaccaaccg gttaataacg
     7981 gaatatttac cctttcggtt tttcaagcca atgcggttca tgatagtgat actggtatct
     8041 ggttttatcc aaatcaaagg tgacatgacc tttcttccga aaaaccgcaa agcaagatag
     8101 cctaaattga agacatgagg gtaaccaacc gcaaattaca gaaaatattt cacgtagcta
     8161 ttgtcctcgc tgtattctta atagggttga atgttggatt attttttccg aaagtttcaa
     8221 attatctaag acaaaatcct ccccaaatta taattacaga gccaaagcgt gattttggtt
     8281 gtgtttatcc tggggtaaca ctaaaacacg attttactat aattaacggg ggcaagcgtt
     8341 cgttatatat caaacgaatt agaacaaatt gtacatgcac acaagcgaga attgacaata
     8401 atgtggttcc cccaaaaaga aaaactaaat taagaatagc tactgttgca tcaaaatata
     8461 aaaccaccat aaatgatttt gtggctctac attcaaatga ccccattaca ccaataaaaa
     8521 tggtttacat taccggtcaa gttactccta acattgaagc gagtccatca gtagtaaatt
     8581 ttggaattgt agaaccctgg gaattaccat taactcaaaa aatcgtcatt tatcgagaac
     8641 cttacggtaa agcaactcat gatgacctca agattgttga agattctgga agacttttta
     8701 caaatcttac aaaactcgac gaaaaaagat gggaactcaa agttacccta cctaaaaacc
     8761 atccgtttgg ttttataata gggaaaattt ggatagaaaa caattcttcg gaaaatttta
     8821 aaatagaaat acctataatg ggtaaaatta taagtagcgt agttataatt ccggatgaaa
     8881 tattttatgg tgtgatagac cctaaaaaag aaatttttca aaaaataaga ataggcggtg
     8941 gaaaagaaat tgttacagat gttcatgtga tttcaaaaag ccctttttta gatgggatgt
     9001 tagatgttaa aattaaaaaa atagccgggg attttcagct tttcactaca cttaattctc
     9061 ataagcttaa acaaaccaaa gatttaattg aaggttgcat atatattaaa gtgaaaacaa
     9121 aagaagggaa agaatatacc ataggcattc ccgttctagg aataataaac tccaccgttg
     9181 taaaccatta gaaattttca agttaaaatt gcctaaaaat tttggggtgt gttcgctaaa
     9241 tccaatcgga atgaaacggc ggccggggag cgggcttccc ggccttctct tcattctggc
     9301 ctggctcctg ctctcgctgg cggccgctct ccgcggccag gaggcgccgg ctcttcccgt
     9361 tatccgcctc tgggtcttcg cctcgaagga ttgcggcgat tgccgggcgg tccagccgga
     9421 gg
//
LOCUS       DAOQYN010000055         9208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8011_length_9208_cov_5.037365, whole genome shotgun sequence.
ACCESSION   DAOQYN010000055 DAOQYN010000000
VERSION     DAOQYN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8011_length_9208_cov_5.037365"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2770)
                     /locus_tag="PKN80_03430"
     CDS             complement(<1..2770)
                     /locus_tag="PKN80_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35097.1"
                     /translation="MKRKTLAILMAVLLLTAGSRAGAQEAAPDPTIKVKTIPIIFSSF
                     RQKRLQVFFMVENSSGRSRELTVRPKLTDFKTSQPVSLGAAPKISLPAGFDGETGFTI
                     PADGLKTWSHWDPQLYLLDLELEENGKPVGRLPQVRFGYREAWTEKGNFYLNGKKLFL
                     FGGNHDGRRNEKEMQFAKMAHFNADTVRGDFFVAQQKTLDLADRNGHYVFGAFLAFDP
                     GDRANLYKIGNHPCIIGYALDSGEVTAPHGHPMQIGAVVPPEVQAADPTYKLVRELNQ
                     KDPGRLYGPYVRGHGGNFRSLMWDLGWGVPVQSQEEWLSFWSQNREKVEPFFPQEFAF
                     VRLGANMARLDRHFGQSAIVEHMSRYLGDEAYRQFDTSMIVSYTPGSKLPDTEPKSNF
                     FYLVKNYMYTRILPVWRVYGLGGALLHVDGHPQGLPTRDGKLTPLGETYRRVADEVYL
                     FLAGPKSDFVSKDHGFYPGETIAKSAILINDSVGPVKAEIEWRAADARGKTVASGKQA
                     LSVGQGEVGFLPIEFKAPAVKTRTLFKISALGRDDTGRTHQPADLEIRVYPRTTFAGR
                     GKSLLLIDDSGETRALLEKMGLKADLVSVDTGRLQETAAKLAAGRLLVIGRNAYPAAA
                     KLFAALPVQDYLRRGLNLVVLEQMNRHALGLKLENTGSRDIFIRAADSPLLAGLENRD
                     LSEWRGESRRLPSYPSFDTESLWWYAGYSYQGQMNAFRQRRAWQWSNKATVATFTYEK
                     PQLGNFRVLLDGGFDLLYTPLVEFQAGRGRILVNSLDLVDHYGSDPVATLLLERILTE
                     YDRPGGRTPAPVGVLSDGAAAQLDAFSVETVRGLTGPVIFLTPADLDQLTEARQKELR
                     AFVQGGGSLLASVLTPEQAARLPVRPDLEDRELFNPDLPADPAFSGLGAGDLFVRAPH
                     KFAAITGIE"
     gene            complement(2824..3600)
                     /locus_tag="PKN80_03435"
     CDS             complement(2824..3600)
                     /locus_tag="PKN80_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35098.1"
                     /translation="MKILIPLLLLGLAANTSGAQPTARTAPPPAPPPTIGIYFHPTPN
                     PAEAILGYEGIKMALEENGFEESVGYVDRLEAASLAGLKVLILPRTHAFPRERPESAI
                     RAALRAFVEKGGGLLLVNESVGWRRIFAAEPPFPEIGRGVGTGDTYQNSVSGGVGPMT
                     IKYVTLAVEPAPHPVTSGLPKEFRALHDLPDLQPGPAGRILARRNGGGAALVAGTIGK
                     GRVLLAAPALGLGERNLEEPPNGAALKLLLNGVRWLAGTE"
     gene            complement(3628..5985)
                     /locus_tag="PKN80_03440"
     CDS             complement(3628..5985)
                     /locus_tag="PKN80_03440"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNQ35099.1"
                     /translation="MDQEIVYRVRPLPEGFLVEAGPESLRLECLTWFQGWPFPPVRSK
                     PASHPRGGFLRPAAGFQELDADFNTEGIRNLAGRQRFGNELEEVQVIHFNGIPGLAWR
                     RVIRGAPDRRVHIGGLVERVRPAEGSRLAPGPETFCFHAENARLYNRYCLGVLEDRTR
                     PPAGASTAYDSQAGTFWAQQRHYRAEVGRSPFQPFPAMIYGHPESDLVLLEASLTQAR
                     LYRYYECGRTEAGDAYLWTQAPKTVNALVLEAGESLAGEWNYLALEDKNGLKHPFDDY
                     LRALENFPARFRGRDGRHGRQVIWGSYNCGIWIDFDQERILKTADYIKENLPRCEWIQ
                     IDAGYDAWDEVRLGSVNNVGIGMAYPGEAADPKKFPEGMPAFTAAIKKRGLRPALWVG
                     FYAGTGRRLYQEHPDWFLPYGIELGVAILDLSRPEVRDFVKSAFRLFIKEWGFEGIKL
                     DFWSYLFEEEALILPAGRMSGTEYRNWLLQTIRELLPEDGYLQLGCDIAMANPHLAAW
                     ADNYRYGRDIGSGQWETVRTNARWAAFCLNTRSGHLFVPNSDAVGVLPGLSDTELRTH
                     LNFCLVSRTLVEIGGWLHQDREHFLMPFLQKAACCPGNGDPVHFAGFDFTAGDEIPAV
                     WYLKGPHFSRLAGNRCLPQRTFGVFNWEESPRNFEISAGSLGLPEDREYLVKDFWSGR
                     VTALEKFRLELAGHASALFGVLEADEAGGILDSNAEITAAAAGADRLTVSLAAAGRVE
                     LTLRRRPARVLENGRPIEARITELEKGAASILFKERGAITVDFEF"
     gene            complement(6026..8080)
                     /locus_tag="PKN80_03445"
     CDS             complement(6026..8080)
                     /locus_tag="PKN80_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HNQ35100.1"
                     /translation="MSGKGFTRPAAVRWLAPVITLLLLPAAAGAAPPVRNLDRRAESR
                     WLNHLLPLPQEIAIRQKVILAPEAIGIRLRDDAGPIEANARRELEAFFKEKTGRLPDG
                     REFEIRLGVLDAAGRLDGRRVAGAERLAGVPNADQAYLIQPEGTRTLQLAGRGEKGLY
                     YAVRTLIQLLEPASGPERVELPLARVLDWPDFEERGLWNCGSLSLDLAPETKFNFSLS
                     NLVLALGQPGEPNRAEVRREEIERGRLRAVRVLPRITHLNFLDAIGLYQAYPELAGRG
                     PGAAAGQYRAHRLGRNLEKENRVPCASNPKLTEILADWLTGAAAAGVAECGGWLSERP
                     AGCDCDTCRSEGQFVWEARAFVNAWRRVVREHPDFRIRIFISTTTDENYEKVLAELPP
                     EVKVERACSLGGERVRHQPRDRFVNPVFDAGAARGGWLASYDAPVTANGKVETPEFIV
                     PMSSPQRVRDFVLQLAGRGYRGVYGMLAGEANDWLNIYALAEWSWNLDGRNERELALA
                     WATRQGYPEPEKAADWVEIMGPIEFDLYDSGFPEIYAWGEAARLVREKRRPRLGEGIF
                     RYFEEPADFERKTAAARTALELARSFPRPELANESRVVLSYLELARQVYRAAELAAEP
                     ARRIEVAGALAAAAAENGAAIRAWRAGLGPEPWHPRVHHAIRAAEETARQISEILNGA
                     PD"
     gene            complement(8070..>9208)
                     /locus_tag="PKN80_03450"
     CDS             complement(8070..>9208)
                     /locus_tag="PKN80_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35101.1"
                     /translation="AGLSVENFASRQAFIRDSASPLLAGLEDEDFSDWRGESRRLPAY
                     QSWNPNSKWNDNPQIPSKYGHSNSFGQGRFWHWTNKGLVATFTYEKPQLGNFRVLLDH
                     EFDLLYTPLIEFRNGKGRMIFSQLDLVDHCENEPVAALLMDRLIRELNRPAGREPEPA
                     GLAGNGPDRNRLEELRIEHREGLAGPVTYLAPEAAVEPAALEKYLKEGGLILVSLRDQ
                     AAADRLPVRITVEKRPYFKADLPAEPFFAGLSRSDFYFRKVVEIPQVTSINGRPAENG
                     TLGVIPVGRGRLVYLQVFPELWKDPWQQGKCRRILSTVLANLGAASRAVPDFRLIGGY
                     GLVNEWLAGYHTEVPALDARPMVKESPLYPKPALDFDPAAHYVW"
BASE COUNT         1440 a         3002 c         3167 g         1599 t
ORIGIN      
        1 cttcgatccc ggtaatggcc gcgaacttgt gcggggcgcg gacgaagaga tcaccggcgc
       61 cgagtccgga aaaggccggg tcggccggca ggtcggggtt gaaaagttcc cggtcttcaa
      121 ggtcgggccg caccggaagc cgggcggcct gttccggggt caagaccgag gccaggagcg
      181 acccgcctcc ctgcacgaag gcgcgcagct ctttctgccg ggcctcggtg agctggtcca
      241 ggtcggccgg ggtcaggaag atgaccgggc cggtcaggcc gcggaccgtt tcgacgctga
      301 aggcgtccag ctgggcggcc gccccgtcgc tcagtacgcc caccggagcc ggcgtccggc
      361 cgccgggccg gtcgtactcg gtcagtattc tttccagcag cagcgtggct accgggtccg
      421 acccgtaatg gtcaaccagg tcgagcgagt tgaccaggat ccggccccgg ccggcctgga
      481 actccaccag cggggtgtag agcaggtcga agccgccgtc cagcaggacc cggaaattgc
      541 ccagctgggg cttctcgtaa gtgaaggtgg ccacggtggc cttgttcgac cactgccagg
      601 cccggcgctg gcggaaggcg ttcatctgtc cctggtagga gtagccggcg taccaccaga
      661 ggctttcggt gtcgaaggag ggataggagg gcagccgccg gctctccccg cgccactcgg
      721 agaggtcgcg gttctccagt ccggccagga gcggcgagtc ggccgcccgg atgaagatgt
      781 cgcgggaacc ggtgttctcc agcttgaggc ccagggcgtg gcggttcatc tgttccagca
      841 ccaccaggtt gaggccgcgg cgcaggtaat cctggaccgg cagggcggcg aagagtttcg
      901 cggcggccgg gtaggcattg cggccgatca ccagcagccg gccggcggcc agcttggcgg
      961 cggtctcctg gaggcggccg gtgtcgacgc tcaccagatc agccttgagg cccatctttt
     1021 cgaggagtgc ccgggtttcc ccggaatcat cgatcagcag cagcgatttt cccctgccgg
     1081 cgaaggtcgt ccggggataa acccgtatct ccaggtcggc cggctggtgt gtccggccgg
     1141 tatcgtcgcg gccgagggcc gagattttga agagcgtcct ggttttcacc gccggcgcct
     1201 tgaactcgat cggcaggaag ccgacctcgc cctggccgac gctgagcgcc tgcttgccgg
     1261 aggcgaccgt cttgcccctg gcgtcggccg cgcgccactc gatttcggcc ttgaccggtc
     1321 caaccgaatc gttgatcagg atggcgctct tggcgatggt ctcgcccggg taaaagccat
     1381 ggtccttgga gacgaaatcg gacttcggtc cggccaggaa gaggtagacc tcgtcggcga
     1441 cccggcggta ggtttcgccc agcggggtga gcttgccgtc ccgggtcggc agtccctgcg
     1501 ggtggccgtc gacgtggagc agggcgccgc ccagcccgta gactcgccag accggcagga
     1561 tccgggtgta catgtaattc ttgaccaggt agaagaaatt gctcttcggc tcggtgtcgg
     1621 gcagcttcga gcccggcgtg taactgacga tcatcgaggt gtcgaactgg cggtaggcct
     1681 cgtcgcccag gtagcgggac atgtgctcga caatggccga ctggccgaag tggcggtcca
     1741 gccgggccat gttggcgccc agccggacga aggcgaactc ctgcgggaag aagggttcca
     1801 ccttctcccg gttctgcgac cagaaactca gccactcctc ctgggactgg accggcaccc
     1861 cccagcccag gtcccacatc agggagcgga aattgccgcc gtggccgcgc acgtagggtc
     1921 cgtaaagacg gccgggatcc ttctggttca gttcccgcac caacttgtag gtcggatcgg
     1981 cggcctggac ttccggcggg accaccgcgc cgatctgcat cgggtggccg tgcggcgcgg
     2041 ttacttcgcc ggaatcgagc gcgtagccga tgatgcaggg atggttgccg atcttgtaaa
     2101 ggttggcccg gtcgcccggg tcaaaggcga ggaaggcgcc gaagacgtag tggccgttcc
     2161 ggtcggccag gtccagcgtt ttctgctggg cgacgaagaa gtcgccgcgg acggtgtcgg
     2221 cgttgaaatg ggccattttg gcaaactgca tctccttttc gttgcggcgg ccgtcgtggt
     2281 tgccgccgaa gagaaagagc ttcttgccgt tcaggtagaa gtttcccttc tccgtccagg
     2341 cctcacggta gccgaagcgg acctggggca gccggccgac cggcttgccg ttctcctcca
     2401 attccaggtc cagcaggtag agctggggat cccagtgcga ccaggtcttg aggccgtcgg
     2461 ccgggatggt gaacccggtc tcgccgtcga atccggccgg cagggaaatt ttcggagcgg
     2521 cgccgaggct gaccggctgg ctggtcttga agtcggtgag cttcggccgg acggtcagtt
     2581 cccggctgcg gccggaggag ttttccacca taaagaagac ctgcagccgt ttttgccgga
     2641 aggaggagaa gatgatcgga atggtcttga ccttgatggt tggatccggg gccgcctcct
     2701 gggctccggc ccggctgccg gccgtcagca gaagtaccgc catcaggatt gccagcgtct
     2761 ttctcttcat cgccgtctcc ttgtctgagg aaaatagaaa cttgtctgaa agtgcgcggc
     2821 cggtcactcg gttccggcca gccaccggac gccatttaaa agcaatttca gagcggcgcc
     2881 gtttggcggc tcctccaggt tccgttcgcc caggcccagg gcgggcgcgg ccagtagtac
     2941 ccgtcccttg ccgatcgtgc cggccaccag cgcggcgccg ccgccgttac gccgggccag
     3001 gatccggccg gccggtccgg gctgaaggtc gggcaggtcg tgcagcgccc tgaattcctt
     3061 tggaagtccg ctggtgaccg ggtggggcgc cggttcgacc gccagggtca cgtacttgat
     3121 ggtcatcgga ccgacgccgc cgctgaccga gttctggtaa gtatcgccgg tgccgacgcc
     3181 ccggccgatc tccgggaagg gcggttcggc ggcgaagatc cggcgccagc cgaccgattc
     3241 gttgaccagg agcaggccgc cgcccttctc tacgaaggcg cgcagggcgg cccggatcgc
     3301 gctctccggc cgctccctgg gaaaggcgtg ggtccggggc aggatcagga ccttcaggcc
     3361 ggccagcgaa gcggcctcca gccgatcgac gtagccgacg ctctcctcga agccgttctc
     3421 ctcgagagcc atcttgattc cctcgtaccc caggatggcc tcggccgggt tcggggtcgg
     3481 atggaaatag atcccgatgg tcggcggggg agccggcggt ggcgccgtcc gggcggtcgg
     3541 ctgggcgccg gaggtattgg cggccaggcc gaggagcagc agcggaatca ggatcttcat
     3601 cgtttttctc attttggacc ggcctcctca gaattcaaag tcaaccgtaa tggcgccccg
     3661 ttccttgaag aggatcgaag cggccccctt ttcgagttcg gtgatccggg cctcgatcgg
     3721 ccgaccgttt tcgagcaccc gggccggccg gcgccgcagg gtcagttcaa cccggccggc
     3781 ggccgccagg gaaaccgtca accgatcggc cccggccgcc gcggcggtga tttcggcatt
     3841 ggagtccagg atcccgccgg cctcgtccgc ttccagcacc ccgaagaggg ccgaggcgtg
     3901 gccggccagt tccagccgga acttttccag ggcggttacc cggccgctcc agaaatcctt
     3961 gaccaggtac tcccgatcct ccggcagccc cagcgagccg gcgctgattt cgaagttccg
     4021 gggcgactcc tcccagttga agaccccgaa ggtgcgctgc ggcaggcacc ggttgccggc
     4081 cagccgggag aagtgcggcc ccttcaggta ccagacggcc gggatctcat cgccggccgt
     4141 gaagtcgaag ccggcgaaat ggaccgggtc gccgttgccg gggcagcagg cggccttttg
     4201 caggaagggc atcaggaagt gctcccggtc ctggtgcagc cagccgccga tctcgaccag
     4261 ggtgcgcgaa acgaggcaga agttgaggtg ggtccggagt tcggtgtcgc tcaaccccgg
     4321 caggactccg accgcgtcgg agttgggcac gaagaggtgt ccgctgcggg tgttgaggca
     4381 gaaggccgcc cagcgggcgt tggtccggac cgtctcccac tggccgctcc cgatgtcccg
     4441 cccgtaacgg taattgtcgg cccaggcggc caggtgcggg ttggccatgg cgatgtcgca
     4501 gcccagttgc aggtagccgt cctccggaag cagttcccgg atggtctgca gcagccagtt
     4561 gcggtactcg gtgccgctca tccggccggc cggcaggatc agggcctcct cctcgaagag
     4621 gtaggaccag aaatcgagct tgatgccttc gaagccccac tccttgatga agaggcggaa
     4681 ggccgatttc acgaaatccc ggacctccgg cctggaaagg tccaggatgg ccacgcccag
     4741 ttcaatgccg taaggcagga accagtccgg atgctcctgg tagagccgcc ggccggtgcc
     4801 ggcatagaag ccgacccaga gggccggccg cagtccgcgt ttcttgattg ccgccgtgaa
     4861 ggccggcata ccctccggaa attttttcgg gtcggccgct tcgcccggat aggccatccc
     4921 gatgccgacg ttgttgacgc tgcccagccg gacctcatcc caggcgtcat acccggcgtc
     4981 gatctggatc cattcgcacc ggggcaggtt ctcctttatg taatcggccg tcttgaggat
     5041 gcgttcctgg tcgaagtcga tccagatgcc gcagttatag gagccccaga tgacctggcg
     5101 gccatggcgg ccgtcccggc cccggaagcg ggcgggaaaa ttctccagcg cccgcaggta
     5161 atcgtcgaaa ggatgcttca ggccgttttt atcttcgagg gccaggtaat tccattcgcc
     5221 cgccaggctc tccccggcct ccagcaccag cgcgttgacg gtcttgggcg cttgggtcca
     5281 gaggtaggcg tcgccggcct cggtccggcc gcattcgtaa taccggtaga gacgcgcctg
     5341 ggtcaggctg gcctccagca ggaccaggtc actctccggg tggccgtaga tcatggccgg
     5401 gaagggttga aagggggagc ggccgacttc ggcccggtag tggcgctgct gggcccagaa
     5461 ggtgccggcc tggctgtcgt aggcggtgga agccccggcc ggcggccggg tgcggtcctc
     5521 gaggacgccc aggcagtaac ggttgtagag gcgggcattc tcggcatgaa agcagaaggt
     5581 ttccggtccc ggcgccaacc gggagccttc ggccggacgc acccgctcga ccagcccgcc
     5641 gatatggacc cggcggtccg gcgcgccgcg gatcacccgc cgccaagcca ggccgggtat
     5701 gccattgaaa tggattacct ggacctcttc cagttcattc ccgaagcgct gccggccggc
     5761 caggttccgg atgccttcgg tgttgaagtc cgcatccaac tcctggaaac cggcggccgg
     5821 ccgcaggaag ccgccccggg gatggctggc cggtttggac cggaccggcg gaaagggcca
     5881 gccctggaac caggtcaggc attccagccg gagcgattcg ggaccggcct cgaccaggaa
     5941 tccttccgga agcggccgca cccggtaaac gatctcttga tccaccttgc ttttcctcct
     6001 gcttccgtgt ctatatttag gtggtttaat ccggtgcgcc gtttaagatt tcggagatct
     6061 ggcgcgccgt ctcttccgcg gcccggatgg cgtgatggac ccgcgggtgc cagggttcgg
     6121 gacccaggcc ggcccgccag gcccggatgg ccgctccgtt ttcggccgcg gccgccgcca
     6181 gcgcaccagc cacttcaatc cgccgggccg gctcggccgc cagttcggcg gcccggtaaa
     6241 cctgccgggc cagttccaga taggatagca ccacccggct ttcattggcc agctccggcc
     6301 tggggaatga ccgggccagt tcgagggcgg tccgggccgc cgctgttttg cgttcgaaat
     6361 cggccggttc ttcaaaataa cggaagattc cctcgcccag gcgcggccgc cgtttttccc
     6421 gaaccagccg ggccgcctct ccccaggcgt aaatctccgg aaagccggaa tcgtagagat
     6481 cgaactcgat cgggcccatg atctccaccc agtcggccgc cttttccggc tccgggtagc
     6541 cctggcgggt cgcccaggcc agggccagtt cccgttcgtt ccggccgtcg aggttccagg
     6601 accactcggc cagggcatag atgttgagcc agtcgttcgc ctcgccggcc agcattccgt
     6661 aaacgccccg gtagccgcgg ccggccagct gaaggacgaa gtcccgcacc cgctgggggg
     6721 agctcatcgg gacgatgaac tccggggtct ccaccttgcc gttggcggtc accggcgcgt
     6781 cgtaactggc cagccagccg ccccgggccg cgccggcgtc gaagaccggg ttaacgaaac
     6841 ggtcgcgcgg ctggtggcgg actctctccc cgcccaggct gcaggcccgt tccaccttca
     6901 cctccggcgg cagttcggcc agcacttttt cgtaattttc gtcggtggtg gtggagataa
     6961 agatccggat ccggaaatca gggtgctccc ggaccacccg gcgccaggca ttgacgaagg
     7021 cgcgcgcctc ccagacgaat tggccctcgg agcggcaggt gtcgcagtcg cagccggccg
     7081 gtcgttcgct cagccagccg ccgcattccg ccactccggc ggcggccgcc ccggttagcc
     7141 agtcggccag gatttcagtc agcttcggat tggaggcgca ggggacccgg ttttcctttt
     7201 caaggttccg gccgagtcgg tgggcgcggt actggccggc ggccgcgccc ggaccgcggc
     7261 cggccagttc cgggtaggcc tggtagaggc cgatggcgtc caggaaattg aggtgggtta
     7321 tcctgggcag gacgcgcacc gcccggaggc gtccccgttc aatctcctcc cggcgtacct
     7381 cggcgcggtt cggttcgccc ggctggccga gcgcgaggac gaggttggaa agggaaaagt
     7441 tgaacttggt ctccggggcc aggtccagag agagggagcc gcagttccag aggccgcgct
     7501 cttcaaaatc cggccagtcc aggacgcggg ccagcggcag ctcgacccgc tccggaccgc
     7561 tggccggttc cagcagctgg atcagggtgc ggacggcgta gtagagtcct ttttcgccgc
     7621 ggccggccag ctggagcgtc cttgttcctt ccggctggat caggtaggcc tggtcggcgt
     7681 ttggcacgcc ggccaacctt tcggcgccgg ccacccgcct tccgtccagg cggccggcgg
     7741 cgtccagtac ccccaaacga atctcgaact cccggccgtc cgggagccgg ccggtctttt
     7801 ccttgaagaa ggcttccagt tcgcgtcgcg cgtttgcctc aatcggaccg gcatcgtccc
     7861 gaagccggat gccgattgct tccggcgcca ggatcacctt ctgccggatg gcgatttcct
     7921 ggggcagggg cagcaggtga ttcagccagc ggctttcggc ccgccggtcc aggttccgga
     7981 ccggcggcgc cgcgccggcc gccgccggca ggagcagcag cgtgatgacc ggcgccagcc
     8041 accggacggc cgccgggcgg gtaaatcctt taccagacat aatgggcggc cggatcaaag
     8101 tccagggccg gcttgggata aaggggactt tctttgacca tcggccgggc gtccagggcc
     8161 gggacctcgg tgtggtaacc ggccagccat tcgttgacca ggccgtagcc gccgatgagc
     8221 cggaagtccg ggaccgcccg gctggccgcg cccaggttgg ccagcacggt gctgaggatc
     8281 cggcggcact tgccctgctg ccagggatcc ttccataatt cgggaaagac ctggagatag
     8341 acgagacggc cgcggcccac cggtatcacg ccgagcgttc cgttctccgc cggccggccg
     8401 ttgatcgagg tcacctgggg aatctccacc accttgcgga aatagaaatc gcttcgggag
     8461 aggccggcga agaacggctc ggccgggagg tcggccttga aataaggcct cttttccacc
     8521 gttatccgga ccggcagccg gtcggcggcc gcctgatccc ggagtgaaac cagaatgagg
     8581 ccgccctcct tcaggtactt ctccagggcg gccggttcga cggccgcttc cggcgccagg
     8641 taagtcaccg ggccggccag cccctcccgg tgctcgatcc ggagttcctc cagccggttg
     8701 cggtccgggc cgttgccggc caggccggcc ggctccggtt cgcggccggc gggccggttc
     8761 agttcccgga tcagccggtc catcaggagg gcggccaccg gttcgttttc gcaatggtcg
     8821 accaggtcca gctgggagaa gatcattcgg cccttgccgt tgcggaactc gatcagcggc
     8881 gtgtagagca ggtcgaactc gtggtccagc agcacccgga agtttcccag ctggggcttc
     8941 tcataggtga aggtggccac caggcctttg ttggtccagt gccagaagcg cccctggccg
     9001 aaggagttgc tatggccgta cttgctgggg atctgcgggt tgtcgttcca cttgctgttc
     9061 gggttccagg actggtaggc cggcagccgg cggctttcgc cccgccagtc gctgaaatcc
     9121 tcatcctcca gtccggccag cagcggcgag gcggagtcgc ggatgaaggc ctggcggctg
     9181 gcgaagttct ccaccgagag gccggcca
//
LOCUS       DAOQYN010000056         9145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8084_length_9145_cov_4.344664, whole genome shotgun sequence.
ACCESSION   DAOQYN010000056 DAOQYN010000000
VERSION     DAOQYN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8084_length_9145_cov_4.344664"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..>294
                     /locus_tag="PKN80_03455"
     CDS             66..>294
                     /locus_tag="PKN80_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ35102.1"
                     /translation="MKRKRFAKKGFTLIELIVVIAIIAILAGIVLPGVMKALQQSKVN
                     RAVSEMQHLATVLGQVYNDIGYYVRLEDLTEK"
     assembly_gap    295..304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <305..684
                     /locus_tag="PKN80_03460"
     CDS             <305..684
                     /locus_tag="PKN80_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35103.1"
                     /translation="QSIRVGPQAYFRIWYNQVDYNLNPSLDENLTVALNSTSPLVLTG
                     NLWNGPYLTYKEYESNRPLDPWGYPYRLFWSQSASVKPAGASGTMIIISSGSNKLLES
                     NYSNFDPNDRGDDVYFSFNWGIQ"
     gene            713..2536
                     /locus_tag="PKN80_03465"
     CDS             713..2536
                     /locus_tag="PKN80_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35104.1"
                     /translation="MEPGRRTDKRGMALVVAILTAFILLALLASLFWIVAATSRQENY
                     ATNAAIALNLAEAGVADAIYRFNYTGKDALGYDTFPFWDSLNALGGSITTDAANLNLS
                     GDLTYLVRGDNTGLKGTLNDGSYYVGLLDDAVGDTITIIAVGEYKGVRRQLKVPLRGR
                     AYTDRPRQYPGQGISEAFNKHAVYARNITCAGTTVSGNLAANNLAGTLPGAATFTRIE
                     SGYFEVPIYSAPFPSPSLVPVIPDKRFRDEDGAVSNYQNTTGAPYVLTTEWKSTYDAW
                     IDYPNSIPSDDQYTVEGANNERWQFEPRTGTDTLVHINYTAAVYPINAVNGHIYYERG
                     DISVPGQTAVSTSSGGNIYLYNTTSTRPNCIGNICAAGSITIRSGTIQGNIEAGGDIL
                     VTGGTITGNLTANGNIILAGGIINGSVYAGGDLAVTNSVGPVTVNAASSNRRAALACR
                     GTLLLAAAPVISLGGSQDTAFLVESGGSDTAIINAVAQIYYANTVPAQAALIAMGNPA
                     IVNLNAGAGFNDSYSPGRSRLIYAGGADSEIRMNGGTFYGILVASGTVTLSGGTLYYL
                     ETPGYSFFILPAGADYREDLFKGFSGGRRAYAPVWKSWSFK"
     gene            2533..3054
                     /locus_tag="PKN80_03470"
     CDS             2533..3054
                     /locus_tag="PKN80_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ35105.1"
                     /translation="MNRIRGYTLIEVVLAVLVVAISVSATLVVMNKMVGYTAVRGYTM
                     DKVNAVAITQYVLDEIRDRRFPPTSTMGRLDGTAFSGTIRLPNDTTGTLYAYTSNSSN
                     GTEYNYTTTIRAYDPAASESRSDVTTDEYDGSSRVDSMLAQRSLLEIKVEVYRGNQLI
                     MRTVTYKTRNGYY"
     gene            3054..3758
                     /locus_tag="PKN80_03475"
     CDS             3054..3758
                     /locus_tag="PKN80_03475"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ35106.1"
                     /translation="MRNQKRHTAAGFTLLEMLTTAAILAIVFGLLAIIFSRATTLHRV
                     VRSGGDAENFGIYVANTILYGPGASWEQGLLAAAEVVNPAATLPAGLALSPKVPSNYG
                     SSPYNPNPYLIFRTMSGDYLIYKLKDDGTTILRARGNNFDETDETTYIDLKPGWASDR
                     QLDLFFDETAPASVVSSGFYYYTKDSNGAPTPITSASDPVYYVKMRFVLRNILQSDQE
                     AVVIERHVRVRNQILF"
     gene            complement(3775..5082)
                     /locus_tag="PKN80_03480"
     CDS             complement(3775..5082)
                     /locus_tag="PKN80_03480"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNQ35107.1"
                     /translation="MYALSLGCPKNLVDTERFLAGRLKAGYRLAESPEKADLLFLNTC
                     AFLTEAVRESESFLEQLASLKRPGRQRLYLAGCLVARQKEELLRRHPAVDRLIYPTEL
                     AGLAGRCLCTPGHYAYLKIAEGCDNRCAYCLIPSLRGSLASYPARRLEAEARALAALG
                     VRELNLVAQDTTAYGRDRGKKDGLARLLERLVKIEELRWIRLLYTYPTGYSERLIECL
                     ARHAIRPDGTTDPERKICAYLDIPLQHAAPRILAAMGRRGGVEEYRRLIERLRREIPG
                     LNLRTSMIVGFPGERESDFLQLLDFIRAIEFEKLGAFAYSAEPGTRAASFPGQVSASV
                     KEERRAEIFRVQREISRRQLSKWLGRETEVLVDGPDPEAPGRSRGRSQYEAPEVDGEV
                     KLAGRWPAGEFLRVKITAIRDYDLVGQPLARGIRGRSGKRAGR"
     gene            5279..7876
                     /gene="mutS"
                     /locus_tag="PKN80_03485"
     CDS             5279..7876
                     /gene="mutS"
                     /locus_tag="PKN80_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNQ35108.1"
                     /translation="MDDFTPMLRQYRELKSQHPDCLLLFRLGDFYEMFDQDARQAAPI
                     LDLVLTSRDAGRSGRIPMCGLPYHALEGYLGRLIKAGLKVAIGEQTEDPAKARGLVKR
                     EVVRVVTAGTFIDEGSPENRCLLCLNPAREGFGLAFVEAAGGTIQANQFENAARAAET
                     VAGLPVYECLFPAGAETASRQFLEQPAFRSRKLTLSPLEDWTFNPELDRRTLQEHFRV
                     ASLAGFGLEERPLALAACGALVEYLRRLNRGQLRHLDSIRLCTVSENLFIAPAAFAGL
                     ELENLFERMDYTRSPLGKRRLRQWLYQPLKKASAIRERQAAVELLRSDPERLEGLEEL
                     LKNLPDVEKNLSRLSGGYGSARDFLGLRNALARIPEIKALADPPAARNPLFSLADLPE
                     LRTELERALDPAAPISQSEGRLVRSGYHPELDGLRDLQRNSREGLRRLQAEEIKRTGI
                     NSLKVGYNRVFGYYLEVSKTNLHLVPEDYLRKQTLVNAERFITPKLKEFEERLLNAEE
                     RILALEAELLAGLRERILEQSAPLHRFAEELARLDALDSLARLAGEPGYVRPEVDEGN
                     GIEIIQGRHPVVEKHLQENFIPNDLFLNTETDHLLIITGPNMAGKSTYIRQAALLVIM
                     AQMGAAVPAGRARVGLVDRIFTRIGVHDDISRGQSTFMVEMSETAEILHNLSERSLVV
                     LDEIGRGTSTLDGLSLAWAIAEELNRHRTRTLFATHFHELTALARGRTGVKNYNVAVR
                     KWRDRILFLHKIVAGGSDESYGIYVAGLAGIPDRVVSRARQLLTQLEAEGSLKEKLFD
                     RSGQRDETPSLFDAAGSEAAAETAAWLEAREEVCRELEKLEIERMTPLEALEKLDRWR
                     KRLEKNGHD"
     gene            7863..>9145
                     /gene="mutL"
                     /locus_tag="PKN80_03490"
     CDS             7863..>9145
                     /gene="mutL"
                     /locus_tag="PKN80_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNQ35109.1"
                     /translation="MAMIKRLPPDLAGKIAAGEVIERPASAVKELVENGLDAGADSIE
                     VQLLNGGKQLILVRDNGSGIDTEDLEAIGERHATSKISRLEDLAAIASFGFRGEALYS
                     LAAVSDFRLRSRTASGESGLEVHIRGNRRMGTAPVAMNRGTEVEVRELFFNTPARRKF
                     LKSDPAEFQLTVNQIIPYTLAYPECRFAIRHQERVRLDLAAAENPMRRFQQVLNLEPE
                     NLLEAGQESAGTGFAFKACLGDVNIQRPRRNLQFVFVNRRPVYSRAFSQAVNQAYRSV
                     MPAGVFPFFALFLELSPELVDVNIHPSKHEVRINGEARLAGLVRVACEQALTTRGRAR
                     RIKLAVFKTGPAAESVFQPKLSESGAGYRPPLARPAAPVPAADTALPRPEAIESPPAA
                     AEATLRELLSNAFFIGAFRRRYLIFEAGSSLLLID"
BASE COUNT         1742 a         3026 c         2722 g         1645 t
ORIGIN      
        1 aatcctgatt gcccgtcttt taaattagta tttatcccac aatttaattt ttgtcggggg
       61 tttttatgaa gagaaaacgg ttcgccaaaa agggattcac gctcatcgaa ttgatcgtgg
      121 tcatcgccat catcgccatc ctggccggta tcgtcctgcc cggagtcatg aaggcccttc
      181 agcagagcaa ggtaaaccgg gccgtctcgg agatgcagca cctggccacg gtgctcggcc
      241 aggtttataa cgacatcggg tactacgtgc ggctggagga tctgaccgaa aaggnnnnnn
      301 nnnntccaaa gcatccgggt ggggccgcag gcctatttcc gcatctggta caaccaggtt
      361 gactacaatc tcaacccgtc tctggatgaa aacctgaccg tggccttgaa ctcgacctcg
      421 cccctggtcc tgaccggcaa cctctggaac ggtccgtacc tgacctacaa ggagtacgag
      481 tccaaccgtc cgctcgaccc ctggggatat ccctaccgcc tcttctggtc ccagagcgcc
      541 tcggtgaagc cggccggcgc cagcggtacc atgatcatca tctcctccgg cagcaacaag
      601 cttctcgaat ccaactattc caacttcgat cccaatgacc ggggcgatga tgtctacttc
      661 tccttcaact gggggatcca gtaagatttg cggcaactcg gagcaaaagg acatggaacc
      721 cggacggcgg acggacaagc ggggcatggc cctggtagtg gccatcctga ccgccttcat
      781 cctgttggct ctgctggcca gcctcttctg gatcgtggcg gccacctccc gccaggagaa
      841 ttacgccacc aacgccgcca ttgccctcaa cctggccgaa gccggcgtcg ccgatgccat
      901 ctaccgcttc aattacacgg gaaaagacgc gctcggatac gacaccttcc ccttctggga
      961 cagcctgaac gcgctgggtg gctcgatcac caccgacgcc gccaatctta acctctccgg
     1021 cgatcttacc tatcttgttc ggggcgacaa taccggtctc aagggcactc tgaatgacgg
     1081 cagttattac gttggcctcc tcgacgacgc cgtcggtgac accatcacca tcatcgccgt
     1141 gggcgaatac aagggggtac gccgccagtt gaaggttccc ctgcgcggcc gcgcttacac
     1201 cgaccgaccc cgccagtacc cgggccaggg catctccgaa gcctttaaca agcacgcggt
     1261 ctatgcccgg aacatcacct gcgccggaac gaccgtgagc ggaaacctcg ccgccaacaa
     1321 ccttgcgggt acccttcccg gggccgccac ctttacccgg atcgagtccg gttacttcga
     1381 ggtccccatt tactccgcgc cttttccttc accctcgctg gttccggtta ttcccgacaa
     1441 gcgctttcga gacgaagacg gtgccgtttc caactaccag aataccaccg gtgcccctta
     1501 cgtccttacc accgaatgga agagcactta cgatgcctgg atcgattacc cgaacagcat
     1561 tccatccgat gaccagtaca cggtggaagg agccaataac gaacgctggc agtttgaacc
     1621 ccggaccggt accgacaccc tggtgcatat caactacacc gccgccgtgt acccgatcaa
     1681 tgccgtcaac ggtcatatct attatgaaag aggcgacatt tcagtacccg ggcagacggc
     1741 cgtttccacc tcttccggcg gtaacatcta cctgtacaac actaccagca cccgtcctaa
     1801 ctgcattggt aacatctgtg ccgccggcag catcaccatt cgaagcggca ccatccaggg
     1861 caacatcgag gcgggtgggg atatcctggt caccggcgga accatcaccg gtaacctgac
     1921 cgccaatggc aacatcattc ttgccggcgg tatcatcaac ggctcggtct acgccggcgg
     1981 cgacctggcc gtcaccaaca gcgtcggccc ggttacggtc aatgccgcct ccagcaatcg
     2041 ccgggccgcc ctggcctgtc gggggaccct gctgctggcg gccgcgccgg tcatctccct
     2101 gggcggcagc caggataccg ccttcctggt tgagtccggc ggctccgaca cggccatcat
     2161 caatgccgtc gctcaaattt actatgccaa caccgtgccg gcccaggcgg ccctgatcgc
     2221 gatgggcaat ccggcgatcg tcaatttgaa cgccggggcc ggatttaatg attcttactc
     2281 tcccggtcgg agccgcttga tttatgccgg cggtgccgat tccgagatcc gcatgaacgg
     2341 cgggactttt tacgggattc tggtagccag cggcaccgta acgctctccg gcgggacact
     2401 ttactacctg gaaacgcccg gttattcctt ttttatcctg ccggccgggg ccgactaccg
     2461 ggaggatctc ttcaagggat tcagcggagg acggcgggcc tacgctccag tctggaagtc
     2521 ctggagtttc aaatgaaccg gatacgcgga tacacgctga ttgaggtggt actggccgtg
     2581 ctggtggtgg ccatttcggt ttcggcgacc ctggtggtga tgaacaagat ggtgggatac
     2641 accgcggtgc gcggctacac tatggataag gtcaacgcgg ttgcgataac ccagtacgtg
     2701 ctggacgaaa tccgggaccg ccgttttccg cctacctcga cgatgggccg gcttgacgga
     2761 accgccttca gtggtacgat ccgtcttccc aatgatacga ccggcaccct ttacgcctac
     2821 acctccaaca gcagcaacgg gaccgaatat aactacacca cgaccatccg tgcctacgat
     2881 ccggccgcca gcgaaagccg cagcgatgta acaactgatg agtatgacgg aagcagtcgg
     2941 gttgattcca tgctggcgca gcggagcctg ctggaaataa aggtggaggt ataccgtggc
     3001 aaccagctga tcatgcgcac ggtaacctac aagaccagga acgggtacta ctgatgagaa
     3061 accagaagag acacaccgcg gccggtttta ctctcctgga aatgctgacc accgccgcca
     3121 tcctggccat cgttttcgga ctgctggcca tcatcttctc ccgggccact accctgcacc
     3181 gggtggtccg cagcggcggg gacgccgaaa acttcgggat ttatgtcgcc aacaccatcc
     3241 tttacgggcc gggcgccagc tgggagcagg gtctgctggc ggcggccgag gtggtcaatc
     3301 cggccgccac cctgccggcc ggactcgccc tttcgcccaa ggtgccttcc aattacgggt
     3361 ccagtcctta taatccgaac ccctacctga tcttccgcac catgtctgga gattatctca
     3421 tttacaagtt gaaggacgac gggaccacca tcctccgggc ccggggcaac aactttgacg
     3481 aaacagatga gaccacctac atcgatctga agcccggctg ggcctccgac cggcagcttg
     3541 atctcttctt tgacgagacc gcgccggcct cggtggtctc ctccggcttt tactattaca
     3601 ccaaggattc aaacggcgcc ccgaccccga ttaccagtgc ctccgatccg gtttactacg
     3661 tcaagatgag attcgtcctg cgcaacatcc tgcaatccga ccaggaagcc gtggtcatag
     3721 aacgccacgt gcgcgtccgc aaccagatcc ttttttaagc ccgcctttcc cgcttcatcg
     3781 tccggccctc tttccggatc ttccccttat tccccttgca agcggttggc ccaccagatc
     3841 gtaatcgcgg atcgcggtga ttttcacccg cagaaactcc ccggccggcc agcgtccggc
     3901 cagtttcacc tcgccgtcca cctcgggcgc ctcgtactgg ctgcgccccc gcgaacggcc
     3961 cggcgcttcc ggatcgggcc cgtcgaccag aacctccgtt tcccggccca gccatttcga
     4021 gagttgccgc cgcgaaatct cccgctgcac ccggaagatt tcggcccgcc gctcctcctt
     4081 gacagatgcc gagacctgcc cgggaaagga ggccgctcgg gtgcccggtt cggccgaata
     4141 ggcgaaagcg cccagtttct cgaactcgat ggcgcggatg aaatcgagca gctggagaaa
     4201 atcggactcg cgctcgcccg gaaagccgac gatcatcgag gtgcggaggt tcaggcccgg
     4261 gatctcccgc cgcaggcgtt caatcagccg tcggtattcc tccactccgc cccggcggcc
     4321 catggccgca agaatccggg gagcggcatg ctgaagcggt atgtccaggt aggcgcagat
     4381 tttccgctcc ggatcggtcg tgccgtccgg acggatggcg tggcgggcca ggcactcgat
     4441 cagccgctcg ctgtaaccgg tcggataggt atagagcagg cggatccacc gcaactcctc
     4501 aattttaacc agccgttcca gcaggcgggc caggccgtct tttttgccgc gatcccgacc
     4561 gtaggcggtg gtatcctggg ccacgaggtt caactcgcgc acgcccaggg cggccagggc
     4621 ccgggcctcg gcctccagcc gccgggccgg gtagctggcc aggcttcccc gcaggctcgg
     4681 tatcaggcaa taggcgcagc ggttgtcgca cccctcggcg atcttcaggt aggcgtagtg
     4741 gccgggcgtg cagaggcagc ggccggccag cccggccagt tcggtcgggt agatgagcct
     4801 atcaaccgcc gggtggcgcc ggaggagttc ttccttctgg cgggcaacca ggcagccggc
     4861 caggtaaagg cgctggcggc ccggccgctt caaggaggcc agctgctcca ggaaggactc
     4921 cgattcccgg accgcctcgg tcaggaaggc gcaggtgttc aaaaaaagca ggtcggcctt
     4981 ttccgggctc tccgccagcc ggtagccggc cttgagccgg ccggccagga agcgctcggt
     5041 gtccaccagg ttcttcggac agcccaggct cagggcgtac acccgccggt taagaggccg
     5101 ccggcgccgg ccggtagccg gttttgaaac ggcagctgga tcagcgggca ttttcataac
     5161 ctcatatttt accggaaaac cggccaactt ccaaacccgc ccatgatccg gaatccaagc
     5221 cgactgggtt ctcaacaaat tttcttttgc cccgcatctt gagatacaat aataaatcat
     5281 ggacgacttt acgccgatgc tccggcagta ccgggaactg aagagccagc acccggactg
     5341 cttgcttctc ttccggctgg gcgatttcta cgagatgttc gaccaggacg cccgacaggc
     5401 cgcgccgatc ctggacctgg tgcttacctc gcgggacgcc ggccgttctg gtcggatccc
     5461 gatgtgcggt ttgccctacc acgcgctcga aggctacctg ggtcgcttga tcaaggccgg
     5521 cctcaaggtg gccatcggcg aacagaccga ggacccggcc aaggcgcgcg gcctggtaaa
     5581 gcgggaggtg gtgcgggtgg tcaccgccgg caccttcatt gacgaaggca gcccggagaa
     5641 ccgctgcctg ctctgcctca acccggcccg ggagggattc ggcctggcct tcgtcgaggc
     5701 cgccggcggc accatccagg ccaaccagtt cgaaaacgcg gcccgggcgg ccgaaacggt
     5761 cgccggactc ccggtttacg agtgcctctt tccggccggc gccgaaacgg ccagccgcca
     5821 attcctggaa cagccggcct tccggagccg gaaactgacc ctgagcccgc tggaagactg
     5881 gactttcaat ccggaactgg accgccggac gctccaggaa cacttccggg tcgccagcct
     5941 ggccggattc ggcctggagg aacggccgct ggccctggcc gcctgcgggg ccctggtgga
     6001 atacctgcgc cgcctgaacc gcggtcaatt gcgccacctg gactcgattc gcctctgcac
     6061 cgtctccgaa aatctcttca tcgcgccggc cgcctttgcc ggcctggaac tggaaaacct
     6121 tttcgagcgg atggattaca cccgctcgcc tctgggcaag cgccgcctgc gccagtggct
     6181 ttaccaaccg ctgaaaaagg cgtcggccat ccgggaacgg caggcggccg tggagttgct
     6241 ccgttccgat ccggaacgac tggaggggct ggaggaactg ctgaagaacc tgcccgatgt
     6301 cgagaagaac ctttcgcgat tgagcggcgg ttatggttcg gcccgcgact tcctcggcct
     6361 gcggaacgca ctggcccgga ttccggaaat caaggccctg gccgatccgc cggccgcccg
     6421 gaaccccctt ttcagcctgg ccgatctgcc ggaactgcgg acggagctgg aacgggccct
     6481 ggatccggcg gccccgatca gccagtcaga ggggcgcctg gtccggagcg gctaccaccc
     6541 ggaactggac gggctgcggg acctgcagcg taacagccgg gagggcttgc gccgcctcca
     6601 ggccgaggaa atcaagcgta ccggcatcaa ttccctcaag gtcggctaca accgggtctt
     6661 cggctattac ctggaggtaa gcaaaaccaa tctgcacctg gtgcccgagg attacctgcg
     6721 caagcagacc ctggtcaacg ccgaacgctt catcaccccg aagctcaagg aattcgagga
     6781 acgcctgctg aacgccgagg aacggatcct ggccctggag gcggaactcc tggccggact
     6841 gcgcgaacgg atcctggagc agtcggcgcc gctgcaccgc ttcgccgaag agctggcccg
     6901 gctggacgcc ctggactccc tggcccggct ggccggcgaa ccgggttatg tccggccgga
     6961 ggtggacgag ggaaacggga tcgaaatcat ccaggggcgc cacccggtgg tggagaagca
     7021 ccttcaggag aacttcattc ccaacgacct gttcttgaac accgagaccg atcacctgct
     7081 gatcatcacc ggaccgaaca tggccggcaa gtcaacctat atcaggcagg cggcgctgct
     7141 ggtgatcatg gcccagatgg gcgcggccgt gccggccggc cgggcccggg tgggcctggt
     7201 ggaccggatc ttcacccgca tcggcgtcca cgacgacatc agccgcggcc agagtacctt
     7261 catggtcgag atgtccgaaa cggccgaaat actccacaac ctctcggaac gcagcctggt
     7321 ggtgctggac gagatcggcc ggggcaccag caccctggac ggcctgagcc tggcctgggc
     7381 cattgccgag gaactcaacc ggcatcggac gcgcacgctc ttcgccaccc atttccacga
     7441 gctgaccgcc ctggcccggg gccggaccgg agtcaagaat tacaacgtgg cggtccgcaa
     7501 gtggcgtgac cggattcttt ttctccacaa gatcgtggcc ggcggcagcg atgaaagcta
     7561 cggcatctac gtggccggcc tggccggcat tccggaccgg gtggtcagcc gggcccgaca
     7621 gctgctgacc cagctggagg ccgagggaag cttgaaggaa aaactcttcg accgttccgg
     7681 ccaaagggac gaaacccctt cgctttttga cgccgccggt tcggaggcgg ccgccgaaac
     7741 cgccgcctgg ctggaggcgc gcgaagaggt ctgccgggaa ttggaaaaac tggaaatcga
     7801 acggatgacg ccgctggagg ccctggaaaa actggaccgg tggcgcaaac ggctggagaa
     7861 aaatggccat gattaaacgc ctgccgcccg acctggccgg gaagattgcc gccggcgagg
     7921 tgatcgaacg gccggcctcg gcggtcaagg aactggtgga aaacggcctg gatgccggcg
     7981 ccgactccat cgaggtgcag ctgctcaatg gcggcaagca gctcatcctg gtgcgtgata
     8041 acggttcagg aatcgacacg gaggatctgg aggcgattgg cgaacgccat gccaccagca
     8101 agatcagccg ccttgaggac ctggccgcga ttgcttcctt cggattccgg ggtgaggctc
     8161 tttacagcct ggccgcggtt tccgacttcc ggctgcgcag ccggacggcc tccggcgagt
     8221 ccgggctgga agtccatatc cggggcaacc ggagaatggg aaccgccccg gtggcgatga
     8281 accgggggac cgaggttgaa gtgcgcgagc tcttcttcaa caccccggcc cggcgcaagt
     8341 tcctgaagtc ggacccggcc gagttccagc tgaccgtcaa ccagatcatc ccctacaccc
     8401 tggcctaccc ggagtgccgg tttgcgatcc gccaccagga acgcgtccgc ctggatctgg
     8461 ccgccgccga aaacccaatg cgccgttttc agcaggtcct gaacctggaa ccggaaaacc
     8521 tgctggaggc cggacaggaa tccgccggaa ccggattcgc cttcaaggcc tgcctgggcg
     8581 acgttaatat ccagcggccg cgccgcaacc tgcagtttgt cttcgtcaac cggcggccgg
     8641 tttacagccg ggccttcagc caggcggtca accaggccta ccggagtgtc atgccggccg
     8701 gggtctttcc ctttttcgcc ctctttctgg aactgtcgcc cgaactggtg gacgtcaata
     8761 tccatccttc caagcatgag gtccggatta atggagaggc ccggctggcc ggcctggtcc
     8821 gggtggcctg cgaacaggcc ctgaccaccc gcggccgggc ccgccggatc aagctggcgg
     8881 tctttaaaac cggcccggcg gccgaatcgg ttttccagcc gaaactttcg gaatccggcg
     8941 ccggttatcg gccgccgttg gcccggccgg ccgcgccggt cccggccgcc gatacggccc
     9001 tgcccaggcc ggaagcgatc gaatcgccgc cggccgccgc cgaggccacc ctgcgcgagc
     9061 tgctttccaa cgccttcttc attggcgcct tccgccggcg ttacctgatt ttcgaggccg
     9121 ggagctccct gctgctgatc gatca
//
LOCUS       DAOQYN010000057         9133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8098_length_9133_cov_4.689028, whole genome shotgun sequence.
ACCESSION   DAOQYN010000057 DAOQYN010000000
VERSION     DAOQYN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8098_length_9133_cov_4.689028"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /gene="uvrB"
                     /locus_tag="PKN80_03495"
     CDS             complement(<1..1607)
                     /gene="uvrB"
                     /locus_tag="PKN80_03495"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNQ35110.1"
                     /translation="MSFELVSDFKPAGDQPRAIEQLAANLERGRQFQTLLGVTGSGKT
                     FTMASVIARLNRPALILSHNKTLAAQLYSEFREFFPRNRVAYFVSYYDYYQPEAYLPT
                     TDTYIEKDASINEDIDRLRMYAVSNLTAHRDVVVVATVSCIYPSGNPEDYAGLTVRLA
                     RGEAADQRRIMRRLVDIQYERNDFDLDRGRFRVRGNRLEIVPAYQEAGLRIELAGGQV
                     ARLTGFDTLTGKELEELDEVFIYPAKQFVTTPDKLERALDSITAELEVRLAELKRQGK
                     LVEAQRLEQRTRYDLEMMAELGYCSGVENYSRHFDGRPAGARPYCLLDFFPEDFLLFV
                     DESHVSLPQIRGMYLGDRSRKATLVDYGFRLPSALDNRPQTWDEFQRFMKSVVFVSAT
                     PGRQELNASGAERLGAASPWVAEQIIRPTGLLDPPMLIRPTASQIDDLVAEIRAQVER
                     SERTLVVTLSKHTAEELSEFLKNLGLKVEYIHYEVDTFRRVELLRQLREARYDVLVGI
                     NLLREGLDLPEVSLVAILEADKEGFLRS"
     gene            1885..2358
                     /gene="nrdR"
                     /locus_tag="PKN80_03500"
     CDS             1885..2358
                     /gene="nrdR"
                     /locus_tag="PKN80_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009728099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNQ35111.1"
                     /translation="MRCPFCGFLEDKVVDTRPSEDEKNIRRRRECLSCSRRFTTYEVI
                     EEKPLMVVKHDNRREPFSREKLLTGVMKSCEKRPVSMTDIEALVDRVSSEIRSRFEKE
                     VPSARIGELVIENLARLDQVAYVRFASVYRKFADITEFEKEIARLKKQEANHGRD"
     gene            2345..4699
                     /gene="nrdD"
                     /locus_tag="PKN80_03505"
     CDS             2345..4699
                     /gene="nrdD"
                     /locus_tag="PKN80_03505"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNQ35112.1"
                     /translation="MDEIKTVKKRDGRIVPMDREKIVNAIFKAAQAMGGSDRSLAEDL
                     ADAVVIYLNREFAGRVPSVEEIQDIVEKVLIKTGHAKTAKAYILYRQRRARIRQIRAG
                     ILPDELKLSAAEIDSLRESTDLALFVRTSKDDIVTWNRERIVDALCRETELSRNIAEI
                     IALEVEEEIVASKIGTLTAPLIREMVNAKLVEYGFEQERRRHTRLGMPLYDVRQVITL
                     PNKENANIPHNPEATNLSLAEAIKKEYALLEVFSPEVADAHLAGDIHLHDLGMCDRPY
                     CSGQSLEYVKKFGLSLPNALSIAKPARHGETLLAHMVKFSAALQGHFAGAIGWDAVNY
                     FFAPYLEGMSDRDIKQLAQMLIYEYSQQAVARGGQAIFSDINIYWEVPDHFEKTPAIG
                     PGGQYTGKTYAEYQPEAQKFAWALFEVYLQGDGLGRPFFFPKPLCHLTNKLFKTPGAE
                     EYLTHICRTAAEKGNTYFVFDRGKTAKVSECCRLSFVLDSTDLSEAATPWKMRFCALQ
                     NVTINLPRAAYRAAGDDTRLFEELDRMLGLVVKAHQQKKAFISRLLSLGDRGPLALLT
                     MKTDGETYLRMNRVSYLIGLLGLNEMVQFHLGRELHEDEAALRFGLKVIAYINLACQR
                     LSRELGLKFVLEQTPAESTAYRMAKLDLAHFPIQAGKVVKGTPENGGVYYTNSTYLNV
                     TAPLAPGEKVKQEGLFHPLIEAGALTHVWLGESKPDPLALANFVKKTFTATQNAQIAF
                     SPEFTACLDCGRVSRGLSKECPYCKNSAREEVPAANSSQTGVKV"
     gene            4696..4995
                     /gene="nrdD"
                     /locus_tag="PKN80_03510"
     CDS             4696..4995
                     /gene="nrdD"
                     /locus_tag="PKN80_03510"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNQ35113.1"
                     /translation="MSRKDFEKYLSENPDQFSFTAREENGAPGLEVKNSFYDTVTFFS
                     NRSIDQRGLNELVAATHAGRNVEHITRVTGYFSKVSGWNKGKVAELIERHRNQVN"
     gene            5092..6300
                     /locus_tag="PKN80_03515"
     CDS             5092..6300
                     /locus_tag="PKN80_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35114.1"
                     /translation="MAETKRLRLGVIGAWGRGRLALHAHDPENGLEVVAGADIDETAR
                     RKFKERFPAALITDDYRRLLERPEIDAVFVTTPDFLHEEHAVAALEAGKDVYLEKPMA
                     ITIEGCDRILRAAFDGRRKLYVGHNMRHFAVMEKMKEIIDRGEIGEVKAGWCRHFVSY
                     GGDAYFKDWHAERRNSTGLLLQKGAHDIDILHWLCGGFSKRVTALGGLTLYDRAPGRR
                     LPERTDRPRWSAEHWPPLSQTGLNPVIDVEDLNLMLMELDNGVFASYQQCHYTPDAWR
                     NYTIIGTRGRIENLGDLPGRAAVRVWNRRNDDYNPFGDIQYFVPAAAGGHGGADPRVV
                     NEFVRYLREGCRIRTSAVAARYSVAAGVQATDSLRSQGAPRLVPEVDPELAAYFNADL
                     IERIAPKEGT"
     gene            6297..6662
                     /locus_tag="PKN80_03520"
     CDS             6297..6662
                     /locus_tag="PKN80_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HNQ35115.1"
                     /translation="MITIEDFRKLELKIGRVLEVQPHPNADKLYLIKVDLGRRVVTGA
                     DGNPAEERDVRELVAGIRAYYAAEALIGKQIVVLANLEPAVIRGITSQGMLLATRDSQ
                     NNLSIVTTERTVEEGSPVS"
     gene            7061..8770
                     /locus_tag="PKN80_03525"
     CDS             7061..8770
                     /locus_tag="PKN80_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35116.1"
                     /translation="MSRPVRYEKFQPRESARLALNYLVSMVDENLDRLPYWMVAPHEN
                     PAFAKHLRVDDAEIVASWYEGIDAVRRLLETPEGAEVQQSFYRHLMKSWGEHGLRFHE
                     PYPWARTIHSQFHEMGYILSALNRILANDPSDHAAEARAAGLVRGLRELVIQRKKIVF
                     WSGDFDIKETLYEFPNDLYVQGRGFDLSCHTGRGEQAIRNGVVLEPLVRRHQLTGDPV
                     ALDLAIGLANHLLGPSRYFNYRMEFFGHVHSSVWVAIGLIKLGRVTGETRYLEKGRLV
                     YDYVRSLSSSAGWVPEYTQWKPMAEEHSETCCIKDMIECALELAEAGFPEYWNDANNF
                     CRNQLVENQIKDGCFVAVDNRIPDNEERTYREIDRRIVGGFSGGAEPNCISLTRFRSI
                     AGCCVGTAPQALQLVWDRTIQWQDRSLTVNFPVDRATGPATVETGYPNEGWLRVTRRE
                     AGPVAIRLYPWMGEGLKGRVNGKPVRIRTKDGLALFPSVPAGGTVELSHRLRSKLTRE
                     FVRGRVFTYLWRGSDVIDVRPQGQPLRIYQRVEGVRKHYPKAPKTRTAGTGFVAAPTQ
                     QKT"
     gene            complement(8785..>9133)
                     /locus_tag="PKN80_03530"
     CDS             complement(8785..>9133)
                     /locus_tag="PKN80_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNQ35117.1"
                     /translation="LVLAVQAGRASEYFTETCRIDFGDPVPRIVGRSLLASQILIAQG
                     LVEQVAVEALDVAVEVDPAGDRAVSRLTVLLTFREPGAAEVGREAREVELDWIKSAGR
                     WRIETLRLTEILR"
BASE COUNT         1758 a         3006 c         2859 g         1510 t
ORIGIN      
        1 gaacgcagga aaccctcctt gtcggcctcc aggatggcca ccaggctgac ctcgggcagg
       61 tcaagccctt cgcgcagcag gttgatgccg accagcacgt cgtacctggc ctcgcgcagc
      121 tggcgcagca gttccacccg ccggaaggtg tccacctcgt aatggatgta ctcgaccttg
      181 aggccgaggt tcttcaggaa ttcgctcagt tcctcggcgg tatgcttgct caaggtgacc
      241 accagggtgc gctcgctccg ttccacctgg gcccggatct cggccaccag gtcatcgatc
      301 tggctggcgg tcggccggat cagcatcggc ggatcgagca ggccggtggg acggatgatc
      361 tgctcggcca cccagggtga ggcggcgccc aggcgttcgg cgccggaggc gttcagttcc
      421 tggcggcccg gcgtggccga aacgaagacg accgatttca tgaaccgctg aaactcatcc
      481 caggtctggg gccggttgtc aagggccgag ggaagccgga atccgtaatc gaccagggtg
      541 gccttgcggg aacggtcgcc caggtacatg ccgcggatct ggggcaggct gacgtggctc
      601 tcgtcgacga agagcaggaa atcctccggg aagaaatcga gcaggcagta aggccgggcg
      661 ccggccggcc ggccgtcgaa gtggcgcgag taattctcca ccccggagca gtagccgagt
      721 tcggccatca tctccaggtc gtagcgggtg cgctgctcca ggcgctgggc ctcaaccagt
      781 ttcccctgcc gcttcagctc ggccagccgg acctccagtt ccgcggtgat cgaatccagg
      841 gcccgttcca gcttgtcggg agtggtgaca aactgcttgg ccgggtagat gaagacctcg
      901 tccagctcct ccagctcctt gccggtcagg gtgtcgaaac cggtcaggcg ggccacctgg
      961 ccaccggcca gttcgatgcg aagaccggcc tcctggtagg ccggcacgat ctccagccgg
     1021 ttgccgcgca cccggaaacg gccgcggtcg aggtcaaaat cgttccgctc gtactggatg
     1081 tcaaccaggc ggcgcatgat ccgccgctgg tcggccgcct cgccccgggc cagccgcacc
     1141 gtgagcccgg cgtaatcctc cgggttgccc gatgggtaga tgcaggagac cgtcgccacc
     1201 accacgacat cccggtgggc ggtgaggttg ctgaccgcgt acatgcgcag gcggtcgata
     1261 tcctcgttga tggaagcgtc cttctcgatg taggtgtcgg tagtgggcag gtaggcttcg
     1321 ggctggtagt aatcgtagta actgacgaaa taggcgaccc ggttgcgcgg gaagaactcc
     1381 cggaactcgc tgtaaagctg ggcggccagc gtcttgttgt gggagaggat cagcgccggc
     1441 cggttgagcc gggcgatcac gctggccatg gtgaaggtct tgccgctgcc ggtcacgccc
     1501 agcagggtct ggaactggcg gccccgctcg aggttggcgg ccagttgctc aatggcccgg
     1561 ggctgatccc cggccggctt gaaatccgat accagttcga agctcatggc agtggttggc
     1621 cccggactcc ggaaacggga aaaagaccgg cggtcttccc gcttcccgcc cgggattttc
     1681 aagtatttat tatatcctta tctgggggcc gccggcaacc ggagccggcc gatttgacaa
     1741 actaaatgtt gtgctttaaa atatctcccc gcacaatata tagtggtctc tcccggccgc
     1801 cgccgggcgg gccgaaaagg cttgacaatc taaatattgt gtcttaaaat atccgcggac
     1861 acaatatatg gggaggttgg gggaatgcgc tgtcctttct gcggttttct ggaagacaag
     1921 gtggttgaca cccgcccgtc cgaggacgaa aagaacatcc gtcgccgacg agaatgcctt
     1981 tcctgcagcc gccgcttcac gacctacgaa gtaatcgagg agaagcccct gatggtcgtg
     2041 aagcatgaca accgccggga gcctttttca cgggagaagc tgctgaccgg cgtgatgaag
     2101 tcctgcgaaa agcggccggt ctccatgacc gacatcgagg cgctggtgga ccgggtgagc
     2161 agcgaaatcc gcagccggtt cgaaaaggag gtcccctccg cccggatcgg ggaactggtc
     2221 atcgaaaacc tggcccggct cgaccaggtg gcttacgtgc gcttcgcctc ggtttaccgt
     2281 aagttcgccg acatcaccga gtttgaaaag gaaatcgccc ggttgaaaaa acaggaagcc
     2341 aatcatggac gagattaaga cggtcaaaaa acgggacggc cggattgtcc cgatggaccg
     2401 cgaaaaaatt gtcaacgcca tcttcaaggc ggcccaggcg atgggcggca gcgaccgcag
     2461 cctggccgaa gacctggccg acgcggtcgt catctacctg aaccgcgaat tcgccggccg
     2521 ggttccctcg gtggaggaga tccaggacat cgtggaaaag gtcctgatca agaccggcca
     2581 cgccaagacg gccaaggcct atatccttta ccgccagcgg cgggcccgca tccgccagat
     2641 ccgggccggc atcctccccg atgaactgaa gctctcggcg gccgaaatcg acagcctgcg
     2701 cgaatcgacc gacctggccc tcttcgtgcg gaccagcaag gacgatatcg taacctggaa
     2761 ccgggagcgc atcgtcgacg ccctctgccg ggagaccgag ctctcccgga acatcgccga
     2821 gatcatcgcc ctcgaggtgg aggaggagat cgtcgcctcc aagatcggca cgctgaccgc
     2881 gccgctgatc cgcgagatgg tgaatgccaa gctggtcgag tacggcttcg agcaggagcg
     2941 gcggcgccat acccggctcg gcatgcccct ttacgacgtg cgccaggtga ttaccctccc
     3001 caacaaggag aatgccaaca taccgcacaa cccggaggcg accaacctct cgctggccga
     3061 ggcgatcaag aaggagtacg ccctgctgga ggtcttctcg cccgaggtgg ccgacgccca
     3121 tctggccggt gatatccacc tgcacgacct gggcatgtgc gaccggcctt actgcagcgg
     3181 ccagtcgctg gagtacgtca aaaaattcgg cctctcgctg cccaacgccc tctccatcgc
     3241 caagccggcc cgccacggcg agacgctcct ggcgcacatg gtgaagttct cggccgccct
     3301 ccagggccac ttcgccggcg ccatcggctg ggacgcggtc aactacttct tcgccccgta
     3361 cctggagggg atgtcggacc gggacatcaa gcagctggcc cagatgctga tctacgagta
     3421 cagccagcag gcggtggctc gcggcggcca ggccatcttc agcgacatca acatttactg
     3481 ggaggtcccc gaccacttcg agaagacgcc ggccatcggc ccgggcggtc agtataccgg
     3541 caagacctac gccgaatacc agccggaggc ccagaagttc gcctgggctc tcttcgaggt
     3601 ctatctccag ggagacgggc tgggccgacc cttcttcttc ccgaagccgc tctgccacct
     3661 gaccaacaag ctcttcaaga caccgggcgc cgaggagtac ctgacccaca tctgccggac
     3721 ggcggccgaa aagggcaaca cctacttcgt cttcgaccgg ggcaaaacgg ccaaggtctc
     3781 ggagtgctgc cgcctctcct tcgtgctcga ctcgaccgat ctctccgagg cggccacgcc
     3841 ctggaagatg cgcttctgcg ccctgcagaa tgtcacgatc aacctgccgc gggccgctta
     3901 ccgggccgcc ggcgacgaca cccgcctctt cgaggagctg gaccggatgc tgggcctggt
     3961 ggtcaaggct caccagcaga agaaggcttt catcagccgc ctgctctccc tgggcgaccg
     4021 cggcccgctg gccctgctca ccatgaagac cgacggcgag acctacctgc gtatgaaccg
     4081 ggtcagttac ctgatcggtc tgctcggcct caacgagatg gtccagttcc acctgggccg
     4141 ggaacttcac gaagacgagg cggcgctccg cttcggcctc aaggtgatcg cctacatcaa
     4201 cctggcctgc cagcgcctga gccgggagct gggcctgaag ttcgtgcttg agcagacgcc
     4261 ggccgagtca accgcctacc ggatggccaa gcttgacctg gcccacttcc cgatccaggc
     4321 cggcaaggtg gtcaagggca ccccggaaaa cggcggcgtt tactacacca actcgaccta
     4381 tctcaacgta accgcgcccc tggcgccggg cgaaaaggtc aagcaggagg ggcttttcca
     4441 cccgctcatc gaggccggcg ccctgaccca cgtctggctc ggcgagtcca agccggatcc
     4501 cctggcgctg gccaacttcg taaagaagac cttcaccgcc acccagaacg cgcagattgc
     4561 tttcagcccc gaattcaccg cctgcctgga ctgcggccgg gtcagccggg gcctctccaa
     4621 ggaatgtccc tactgcaaga actccgcccg ggaagaagtc ccggcggcca actcatcaca
     4681 aacgggggta aaggtatgag ccggaaagat ttcgagaagt acttgagcga gaatcccgac
     4741 cagttttcct tcacggcccg ggaagaaaac ggcgccccgg gcctggaagt caaaaacagc
     4801 ttctacgaca cggtcacctt cttctccaac cgttccatcg accagcgcgg actcaacgaa
     4861 ctggtcgcgg ccacccacgc cggccggaac gtcgagcaca tcacccgcgt caccggctac
     4921 ttctccaagg tgagcggctg gaacaagggc aaggtggccg aactgatcga acgccaccgc
     4981 aaccaggtca attaaatcct cccgcgtatc ggcgtgccgg ccgcaaccgg ccggcacgcc
     5041 cgctccttaa accggtttca cccggcacga ttccgaaaaa ggagatttcc cttggccgaa
     5101 acgaaaagat tgcgcctggg cgtcatcggg gcctgggggc gcggccggct cgccctgcac
     5161 gcccacgacc cggaaaacgg gctggaagtg gtcgccggcg ccgacatcga cgaaaccgcc
     5221 cgccgcaaat tcaaggaacg cttcccggcc gccctgatta ccgatgacta tcgccggctc
     5281 ctggaacggc ccgaaattga cgcggtcttt gtcaccaccc cggattttct ccacgaggaa
     5341 catgccgtgg ccgccctcga ggcgggcaag gacgtttatc tcgagaagcc gatggccatc
     5401 accatcgagg gctgcgaccg catcctgcgg gccgccttcg atgggcggcg caagctctat
     5461 gtcggacaca acatgcgcca cttcgcggtg atggagaaga tgaaggagat catcgaccgg
     5521 ggggagatcg gcgaggtgaa ggccggctgg tgccgacatt tcgtaagtta cggcggtgac
     5581 gcctacttca aggactggca cgccgaacgc agaaacagta ccgggctcct gctccagaaa
     5641 ggagcccatg acatcgacat cctgcactgg ctctgcggcg gtttctcgaa acgggtaacc
     5701 gccctgggcg gcctgaccct gtacgaccgg gccccgggcc gccgcctccc ggaacgcacc
     5761 gaccggcccc gctggtcggc cgagcactgg ccgccccttt cgcagaccgg cctcaacccg
     5821 gttatcgatg tcgaggacct gaacctgatg ctcatggaac tggacaacgg ggtcttcgcc
     5881 tcctaccagc agtgccacta cacgcccgac gcctggcgca actacaccat catcggcacc
     5941 cggggccgca tcgagaacct gggcgacctg ccgggccggg ccgcggtccg ggtctggaac
     6001 cgccggaacg acgattacaa tcccttcggt gacatccagt atttcgttcc ggcggcggcc
     6061 ggcggccacg gcggggccga cccgcgcgtc gtgaacgaat tcgtgcgata cctgcgcgag
     6121 gggtgccgga tcaggacctc ggcggtggcc gcccgctaca gcgtggccgc cggcgtccag
     6181 gccaccgatt ccctgcgcag ccagggcgcg cccaggctgg tcccggaagt ggacccggaa
     6241 ctggccgcct attttaacgc cgacctgatc gaacggatcg ctcccaagga gggcacatga
     6301 tcaccatcga ggatttcagg aagctggaat tgaaaatcgg gcgggtcctg gaggtgcagc
     6361 cgcatccgaa tgccgacaag ctctacctga tcaaggtcga tctcggccgc cgcgtcgtga
     6421 cgggggcgga cggcaacccg gccgaagagc gcgacgtccg ggaactggtg gccgggatcc
     6481 gcgcctacta tgcggccgag gcgctgatcg gcaaacagat cgtcgtgctg gccaacctgg
     6541 aaccggccgt catccggggc atcaccagcc agggcatgct gctggccacc cgggattccc
     6601 agaacaacct gtccattgtc accactgaac ggacggtcga agagggcagc ccggtaagct
     6661 gagctccggc ggtccggtac cggccagaga aaagcccata cccgcggtgg gggattacac
     6721 gggtatgggc tttctgttgc ccatccgcgg aacctttttc tgcgttgact tccgtcgctg
     6781 ccggcccgcg gatgggactt tttcaacctt taagttttcg ggtgcgaccc gttccgaaaa
     6841 gggccgcggc gccgcgtgcc tttttcggtt ttaataatta gtggccggtc cgttgaaaaa
     6901 cgtttaaaac cacccggtcc ctcccggaac gcggctgaaa aggagacggc cgtttcccga
     6961 aagggcggcg ccgggatata atttataagg aacgcaatcc gtggcgggcc ggaaccggaa
     7021 gatcccggac tgccgtctct ccacaaaaca ggagccgtct atgagccgcc cggtccgtta
     7081 tgaaaaattc cagccgcgcg aatccgcccg gctggccctc aattacctgg taagcatggt
     7141 cgatgaaaac ctcgaccgcc tgccctactg gatggtggcc ccgcacgaga acccggcctt
     7201 cgccaagcac ctgcgggtgg acgacgccga gatcgtcgcc tcctggtacg agggcatcga
     7261 cgcggtccgg cgcctcctcg agaccccgga gggggccgag gttcagcaat ccttctatcg
     7321 ccacctcatg aaatcctggg gggagcacgg actccgtttc cacgaaccct atccctgggc
     7381 ccggaccatc cacagccagt ttcacgaaat gggctacatc ctctcggccc tgaaccggat
     7441 tcttgccaac gatccttccg accacgcggc cgaggccagg gcggccgggc tggtgcgcgg
     7501 cctgcgcgag ctggtcatcc aacgcaagaa gatcgtcttc tggtcgggcg atttcgacat
     7561 caaggagacc ctctacgagt tccccaacga cctctatgtc caggggcgcg gcttcgatct
     7621 ttcctgtcac accgggcggg gcgaacaggc gatccgcaac ggggtggtgc tcgaaccgct
     7681 ggtacgccgc caccagttga ccggggaccc ggtcgccctc gacctggcga tcgggctggc
     7741 caaccacctg ctgggcccct cccgctactt caactaccgg atggagttct tcggtcatgt
     7801 ccactcgtcg gtctgggtgg ccatcgggct gatcaagctg ggacgggtca ccggcgaaac
     7861 ccgctacctg gaaaaagggc gcctggtcta tgattatgtc cgttccctct cctcctcggc
     7921 cggctgggtt ccggaataca cccagtggaa accgatggcc gaagagcact cggagacctg
     7981 ctgcataaag gacatgatcg agtgcgccct ggaactggcc gaggccggct tcccggagta
     8041 ctggaacgac gccaacaact tctgccgcaa ccagctggtc gagaaccaga tcaaggacgg
     8101 ctgcttcgtg gcggttgaca accgcatccc ggacaacgag gaacggacct accgggaaat
     8161 cgaccggcgc atcgtgggcg gcttctccgg cggcgccgaa ccgaactgca tctcgctgac
     8221 ccgtttccgc tccatcgccg gctgctgcgt cggaaccgcg ccccaggccc tccagctggt
     8281 ctgggaccgg accatccagt ggcaggaccg gtcgctgacg gtcaacttcc cggtggaccg
     8341 ggcgaccggc ccggccacgg tggagaccgg ttatccgaac gagggctggc ttagggtgac
     8401 gcggcgggag gccggaccgg tggcgatacg cctctatccc tggatgggcg aaggcctgaa
     8461 aggccgggtg aatgggaagc cggtccgcat ccggacaaag gacgggctgg cgctcttccc
     8521 gtcggtaccg gccgggggaa cggtcgaact gagccaccgc ctgcggtcaa agctgacccg
     8581 ggaattcgtc cgcggccggg tcttcaccta cctctggcgg ggcagcgacg tgatcgacgt
     8641 ccggccgcag ggacagccgc tgcgcattta ccagcgggtg gagggagtcc gcaagcatta
     8701 cccgaaggcc ccgaagaccc ggacggccgg aaccggtttc gtggccgcgc caacccagca
     8761 gaaaacctga aaagaggcgg gattttaacg caggatttcg gtcaggcgca gggtttcaat
     8821 tcgccagcgg ccggcggatt tgatccagtc aagttcgact tcgcgcgcct cccggccgac
     8881 ctcggcggcg cccggttcgc ggaaggtcag gagcacggtc agtcggctga cggcccggtc
     8941 cccggccgga tccacctcga ccgcgacgtc cagggcctcg acggccacct gttcgaccag
     9001 accctgggca atcagtatct ggctggccag caggctgcgg ccgacaatcc gcggcaccgg
     9061 gtccccgaaa tcgatccggc aggtctcggt gaaatactcg gaggcccggc ccgcctgaac
     9121 cgccaggacc agc
//
LOCUS       DAOQYN010000058         9073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8163_length_9073_cov_4.965070, whole genome shotgun sequence.
ACCESSION   DAOQYN010000058 DAOQYN010000000
VERSION     DAOQYN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8163_length_9073_cov_4.965070"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="PKN80_03535"
     CDS             complement(<1..1437)
                     /locus_tag="PKN80_03535"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ35118.1"
                     /translation="MRDIAITTTRRILALLLLAASAAAAFAGPADRLYQLGLAANPAD
                     IPVFLQALTDRDLAVRKAAASQLIFVNAPEALGPIKTALQAEKSTFVRRCLIEAASRL
                     GTPEARAVLEAALKDAAEINRLAAAERLLRAGDHAAARAEVLTALKSHDRESVLMACY
                     IAGANRMEEAAETLAAIAMAEENGGENSRDTFALRALNRIAERHPSARPQALKAARAK
                     TATISPFMRVELARIIGNFGEAGDLDTLHGWVGGQWVETQLPATRGLARLKNPASVEP
                     VLEALKLALRPRGLYPTHFRIFTEHIRTLAEIGDPRAIEPLKKFIADLPAESGGKPVV
                     QTRILAVNAAGTRLALGEAAAAGELAAFLRDGDASVRREAAKRAVALPDNRLPAEVGA
                     ALAEAAATETEPYTALVLRRAAAARGLPAAAGPAPVILPAEADTTAADTEPRYVMINL
                     DDSSTIDAMERVLAMCEFCDRQDRRLVRD"
     gene            1634..2590
                     /locus_tag="PKN80_03540"
     CDS             1634..2590
                     /locus_tag="PKN80_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNQ35119.1"
                     /translation="MARQIRIEFPGAIYHVTARGNEWQAIFRNNLDRQTFLVLLGQIS
                     EQYGVKVRAYCLMPNHYHLILETPRGNLSQAVGWLQLTYTVRFNRQHKRSGHLFQGRF
                     KAFLVDADEYAQVLTLYVHLNPVRPEDKNAAIPAGRIKTLGNYRWSSHLAYAGKVGKP
                     DWLDISWLQYWGETEDRARKEYQEAIRGLFGKVIVNPQGNVQDSLVLGGDALREQVEN
                     AIKNKSGKEEKHWIIEKSHLDIRRQLEAILKEELDQGVRLWARVRLGGERMIDLSREF
                     GYKDGSGVLHAVKRIERRAEKDQGIQRKLLDLKSRLLSSFKS"
     gene            complement(2667..5510)
                     /locus_tag="PKN80_03545"
     CDS             complement(2667..5510)
                     /locus_tag="PKN80_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35120.1"
                     /translation="MKRSAAGLTFFFALLALGNAAFGQAAGAGKNILWDGGFEIGYGN
                     NFWGAPNGNYGPNRRDMWSNGVIKLNSRIASRIYWLEEGTYTLGAWVKRGPEAADPNQ
                     NFALRLLLTNLNYYDDKQRNEYGKVFPVAAGEGWQRVGWSIEIQGPVRNYFHVEIGPA
                     ENVGPGILLDGVSLTRGTELPEKLPAAAAVEAGFWIPEETGIYVDGEERVVELVIRNN
                     GAARPARVRWEVYDWQNKLVKQDALNENLPAAATLRRKLPLADLPWGGYRLACSVEGE
                     PVLGDALLTLVPRIDQEAFPQYGGDAAVNLASKDFTGRFLKRIGMKVANTLSPGGGVG
                     RWTGVNPAEGEYNWQQEPAVDAVRKQGGVEVVGFLGLKYPPAWVVAKYMKDGQVVDEA
                     GFIKAYADYVYAFVKHYAGRITRITLEDEIGGSYGGPVKDSLLMRVYAAAYAAAKRAA
                     REAGTTISVGINSTRPAWWSDFLDLVDRKQLDYVSQNTLLRPGWSAETLNIMRQKGAY
                     PEYFYTVGVGQRSQLRGTSLILDGASSSGNPPGIFAWQLMMHAWLSRPYGTEDPKDGP
                     LVSAGFYDLRTLGQSVYLPLGGKTGIEYDNSPTLGMQWMAMQKYWLSGMRPARDPKQA
                     YSLNGEPTGHERLFVYPFRNREKTVVVLTTVNGTDNNSQDFDCHWKLSGLDFNRYRPA
                     DIYGQPLKPAADGTLLVPQLPVLFTGLAAADLPAALANFKEMKAEPAASTGARQLAVG
                     KYTLEINPDRPGYLRLMVKEGGRERTLIDGLVGQPELPKPAVEVADGRLEAKATLAFD
                     SKRYLSLTVSEQGVTMRWRQDNTERAEVKQVLRFRIPNEGAGREIVIQEGPKVIAGLL
                     REDYGQLTPAAALPAPAALHPDASTVSLKGFGDYYLSGATGQGGFTPKTGFRWRTSDG
                     QALLEANYTISAYPGGGTRGIQRIIMDLQISAP"
     assembly_gap    5634..5643
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5644..5974)
                     /locus_tag="PKN80_03550"
     CDS             complement(<5644..5974)
                     /locus_tag="PKN80_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNQ35121.1"
                     /translation="MKKTFSAGGVVLNRRNQVLVVNQRGRAWSLPKGHLEPGEEPLAA
                     ARREIHEETGLKNLRHLGELGGYRRFRLDAEGGDDTSELKCITLFLFRAREERLAPRD
                     PDNPEARW"
     gene            complement(5979..8171)
                     /locus_tag="PKN80_03555"
     CDS             complement(5979..8171)
                     /locus_tag="PKN80_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNQ35122.1"
                     /translation="MSVREAVLVFPKPKSILRRPGRLTPVNRLFVLPGGIKPKEVARV
                     FEIAGRHGSSVPELAAGHPAAAAWRLETRREAWTTEKLPAERLERYQREQGYRLEVQS
                     RRHRILVVSESETGLANGVKTLAQLFRRENGRTLLPPVEVVDWPDFRERGPFYESIFG
                     TNLMGLEDWKRALEKLYLLKMNSVSVSLYNCWRRSADLEFLLFRSRKFPELKTLNQYY
                     DHAEGRVRKHYPRMYEEDFLAELVRYARELGLNFIPYFSSLGHNTYLPKAFPEVSMKD
                     EAGRPTGYGFCTENRKTYELLFALYDEILETLKPYGITDFGLGLDEVYARCRCPECQK
                     RPGRGMADFFIEHLVRCAGHLKSRGIKRIIFWYDILYRQGNFNDRLMEILEKEGLRRH
                     LAVGWWCYENPDRAPDLSPRKWLFFWKVDRRLASLVNPSLCLNHLTPERELPPVSSHT
                     DSLARLNSLGKRAGSYGVLSYSHDDPHRWDQYCCLSEFSWNQEGAGVKEFDDRFVAFL
                     FGRRAARYEKIRDVYRRLLDTFGALTEPIHASFRYGARPPARVGQVVSAFADRCPLTP
                     AQWRAFIAAGEKADRELQRLIAGLPEGDYRAAVADRAGQDLRRLLTTVRLAYHAMDFN
                     RHYGYLRQGRAERRTCLAEMARQAGRLEEALAGHRALMDEIAGRRAAYHAPFVLEPMR
                     HENETFGRYLARYRELLARLEESGQDILPTIDFYHDDFFGTDRDYRPF"
     gene            8379..>9073
                     /locus_tag="PKN80_03560"
     CDS             8379..>9073
                     /locus_tag="PKN80_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ35123.1"
                     /translation="MKRGNPNTWLKARAAEEIEWYLSSADVIVVERKRSIRLLGELFA
                     HHFSEPAGLRVLDLGGGDGLVTEYFSNQYPRNDFCLMDGSVAMLDQAKARLKGRPIRF
                     VEKSFEAYAQKGLDPDGYDFIFSSMAIHHLPLKEKGALYARVHRELRGNGLFLVFDVV
                     APVSPKSERLQFRMWVDWMNEALRRSNFAGEVGKFDGFPAVYKAKAENHPSRLLDQLD
                     QLKKAGFKNVDCF"
BASE COUNT         1492 a         2841 c         3074 g         1656 t
ORIGIN      
        1 gtcgcgaacc agccggcggt cctggcggtc gcagaactcg cacatggcca ggacccgttc
       61 catcgcgtcg atcgtggagc tgtcatccag gttgatcatg acgtagcggg gttcggtgtc
      121 ggccgcggtt gtgtccgctt ccgccgggag aattaccggg gccggtccgg ccgcggccgg
      181 cagcccccgg gccgcggcgg cccggcgcag caccagggcc gtgtagggtt cggtttcggt
      241 cgcggccgct tcggccaggg cggcgccgac ctccgccggc aggcggttgt ccgggagggc
      301 cacggcgcgc ttggcggcct cgcgtcgcac cgacgcgtcg ccgtcgcgca ggaaggcggc
      361 cagttccccg gcggcggccg cctcaccgag cgccagccgc gttccggccg cgttgaccgc
      421 caggatccgg gtctggacga cgggcttgcc gccgctctcg gccggcaggt cggcgatgaa
      481 cttcttgagc ggctcgatgg cccgcgggtc accgatctcc gccagagtcc ggatatgctc
      541 ggtgaagatc cggaagtggg tcgggtagag cccgcgcggc cggagggcca gcttcagcgc
      601 ctccaggacc ggttcgaccg aggcggggtt cttcagccgg gccaggcccc gggtggccgg
      661 gagctgggtc tcgacccact gaccgccgac ccagccgtgc agggtgtcga ggtcgccggc
      721 ctccccgaag ttcccgatga tccgggccag ctcgacccgc atgaacgggc tgatggtcgc
      781 ggtcttggcc cgggccgcct tgagcgcctg cggccgggcg gaaggatggc gttcggctat
      841 ccggttcagc gcccgcaggg cgaaggtgtc ccggctgttc tcgccgccgt tctcctcggc
      901 catcgctatg gcggccaggg tctcggccgc ttcctccatc cggttcgcgc cggcgatgta
      961 acaggccatc aggaccgatt cgcggtcgtg cgatttcagg gcggtcagca cctcggcgcg
     1021 cgcggcggcg tgatcgccgg cgcgcaggag gcgttccgcg gccgccaggc ggttgatctc
     1081 ggcggcatcc ttcagggcgg cctcgagcac ggcccgggcc tccggcgtgc cgaggcggct
     1141 ggcggcctcg atgaggcagc gcctcacgaa ggttgatttt tcggcctgga gcgcggtctt
     1201 gatgggaccg agcgcctccg gcgcgttgac gaagatcagc tgggaggcgg ccgccttccg
     1261 gaccgccagg tcgcggtcgg tgagcgcctg caggaagacg gggatgtcgg ccgggttggc
     1321 ggccaggcca agctggtaga gccggtcggc cggcccggcg aaggcggcgg cggccgaggc
     1381 cgccaggagc aggagcgcca gtatccgccg ggtcgtcgta atggcgatgt cacgcatgga
     1441 agcttccttt cgagtttcgc gggtgcttat cccttgcgcg ggaaaggccg gtcccggtac
     1501 ttttataatc tttagtatag cacagaaaac cggctgtttt gctccgttta gccggtcagc
     1561 ccatagttta ggttagtttg gggtcaactc ttgaaacttg acacataggg ttggttctgt
     1621 taaagttttg gtcatggcca gacaaattcg aatagaattt cccggtgcta tctatcatgt
     1681 gacggcacgt ggcaacgagt ggcaggcaat attccggaac aacttagacc ggcagacttt
     1741 tttggtttta cttggacaga tatcggagca atatggagtc aaggtgcgcg cctactgtct
     1801 gatgccgaat cattaccatc ttatcctcga aacaccaaga ggcaatcttt ctcaagccgt
     1861 cggttggctt caattgactt acacggttcg atttaaccga caacacaaaa gatctgggca
     1921 tctttttcaa ggaagattca aggctttttt ggtggatgcg gatgaatatg cccaagtact
     1981 gactttatac gtgcatctta accccgttag gcctgaagat aaaaatgcag ccattccggc
     2041 cgggcgcatt aaaactctgg gcaattaccg atggagcagt cacctggcgt atgctggcaa
     2101 ggttggaaaa cccgactggt tggatatatc gtggttgcaa tactggggtg aaacagagga
     2161 ccgcgcgcgt aaggaatacc aggaagcaat acgagggttg tttggcaagg ttattgttaa
     2221 tccgcaggga aatgttcaag atagtctggt attgggcggt gatgctttgc gtgaacaggt
     2281 tgaaaatgcc attaaaaaca aatccgggaa agaagaaaaa cactggataa tagaaaaaag
     2341 tcatttagat attcgccggc aacttgaagc aatattaaag gaagaattag atcaaggtgt
     2401 ccggttatgg gcgcgggtgc gcttgggtgg ggaacggatg attgatttaa gtagagagtt
     2461 tggatacaag gatggatccg gggttttaca tgcggttaaa cgaatcgaac gtagggccga
     2521 gaaagatcag ggaatacaaa gaaagttgct ggatttaaaa tcccgccttc tgtcaagttt
     2581 caagagttga ccccacctag ggtgtccggc caaaaaaagg ggcggccgtc gggccgcccc
     2641 ttatggtatt cgatctcccg gttcttctat ggagccgaga tctggaggtc catgatgatg
     2701 cgctggatgc cgcgggtgcc gccgccggga taggcgctga tggtgtagtt ggcctccagc
     2761 agggcctgtc cgtcggaggt ccgccagcgg aagccggtct tgggcgtgaa gccgccctgg
     2821 ccggtggccc cggagaggta gtagtcgccg aagcccttga ggctgaccgt gctggcgtcc
     2881 gggtgcaggg ctgccggcgc cggcagggcg gccgccgggg tcagttgtcc gtaatcctcg
     2941 cgcagcaggc cggcgatcac cttcggcccc tcctggatga cgatctcccg tcccgctccc
     3001 tcgttgggga tgcggaagcg aagcacctgt ttcacctcgg cccgttcggt gttgtcctgg
     3061 cgccagcgca tggttactcc ctgctcggag acggtgagcg agagataacg cttgctgtcg
     3121 aaggccaggg tcgccttggc ctccaggcgg ccgtccgcta cctcgacggc cggcttgggc
     3181 agctccggct ggccgaccag gccgtcgatc agggtccgtt ccctgccccc ttccttgacc
     3241 atcagccgca ggtagccggg ccggtccggg ttgatctcca gggtgtactt gccgaccgcc
     3301 agctgccggg cgccggttga ggcggccggt tcggccttca tctccttgaa gttcgccagc
     3361 gccgccggca ggtccgcggc ggccaggccg gtgaagagca ccggcagctg ggggaccagc
     3421 agcgtcccgt cggcggccgg cttcaatggc tggccgtaga tgtcggcggg ccggtaccga
     3481 ttgaagtcga ggccggagag cttccagtgg cagtcgaagt cctgggagtt attgtcggtc
     3541 ccattcacgg tggtcaggac caccaccgtc ttctcccggt tccggaaggg atagacgaag
     3601 aggcgctcgt ggccggtcgg ctcgccgttg agcgagtagg cctgcttcgg gtcgcgcgcc
     3661 ggccgcatcc cgctcagcca gtacttctgc atcgccatcc actgcatgcc cagggtcggc
     3721 gagttatcat actcgatccc ggtcttgccg cccaggggca ggtaaaccga ctggcccagg
     3781 gtgcgcaggt cgtagaagcc ggcgctcacc agcgggccgt ccttggggtc ctcggtgccg
     3841 taggggcgcg aaagccaggc atgcatcatc agctgccagg cgaagatgcc cggcgggtta
     3901 ccgctggagg aggcgccgtc caggatcagc gaagtgcccc gcagctggct ccgctggccg
     3961 accccgaccg tgtagaagta ttccgggtag gcccctttct ggcgcatgat gttcagcgtc
     4021 tcggccgacc agcccggccg cagcagggtg ttctggctta cgtaatcaag ctgcttccgg
     4081 tcgaccaggt cgaggaagtc ggaccaccag gcgggccggg tggagttgat accgaccgat
     4141 atggtcgtgc cggcctcccg ggccgcccgc ttggccgccg cgtaagcggc cgcgtaaacg
     4201 cgcatcaaaa gcgaatcctt gaccgggccg ccgtaggagc cgccgatctc atcctcgagg
     4261 gtgatccgcg tgatgcggcc ggcgtagtgc ttcacgaagg catagacata atcggcgtag
     4321 gccttgatga agccggcctc atcgaccacc tggccgtcct tcatgtactt ggcgaccacc
     4381 caggccggcg ggtacttgag accgaggaag ccgaccactt cgacgccgcc ctgcttgcgc
     4441 accgcgtcca ccgccggttc ctgctgccag ttgtactctc cctcggccgg attgacgccc
     4501 gtccagcggc ccactccgcc gcccgggctg agcgtgttgg cgactttcat gccgatccgt
     4561 ttcaggaagc ggccggtgaa atctttggaa gccaggttga cggccgcgtc gcccccgtac
     4621 tgcgggaagg cctcctggtc gatccggggg acgagggtca gcagggcgtc acccagcacc
     4681 ggctcaccct ccaccgagca ggccagccgg tagccgcccc agggcaggtc ggccagcggc
     4741 agcttccgcc gtagcgtggc cgcggccggc aggttttcgt tcagcgcgtc ctgttttacc
     4801 agcttgttct gccagtcgta gacctcccag cgcacccggg ccggccgggc cgcgccgttg
     4861 ttccgaatga ccagttcgac cacccgctcc tcgccgtcca cgtagatgcc ggtctcctcc
     4921 gggatccaga agccggcctc cacggccgcg gccgccggca gcttctccgg cagctcggtc
     4981 ccccgggtca ggctcacccc gtccagcagg atgcccgggc cgacattctc ggccggaccg
     5041 atctcgacgt gaaagtagtt gcggaccggc ccctggatct cgatgctcca gcccacccgc
     5101 tgccagccct cgccggctgc caccgggaag accttgccgt actcgttccg ctgcttgtcg
     5161 tcgtaataat tgaggttggt caggagaagg cgcagcgcga aattctggtt tgggtcggcc
     5221 gcctccggcc cccgcttcac ccaggcgccg agggtatagg tcccctcctc cagccagtag
     5281 atgcggctgg cgatccggga gttcagcttg atcacgccgt ttgaccacat gtcgcgccgg
     5341 ttggggccgt aattgccgtt cggcgcgccc cagaagttgt tgccgtagcc gatctcgaag
     5401 ccgccgtccc agaggatgtt cttcccggcg ccggccgcct ggccgaaggc cgcgttcccc
     5461 agggcaagaa gagcgaaaaa gaatgtcaag ccagccgctg atcttttcat gccttctcct
     5521 ttttgggatc gcgagagctc tttcttaaat atagtttcgg tcctggtgcg aaccgtttag
     5581 tccgggtcaa ccgtggcagc agctcctgga agaacttccg gtcgcgggga tggnnnnnnn
     5641 nnncccagcg ggcctccggg ttgtccgggt cgcgcggggc cagccgctcc tcccgggcgc
     5701 ggaagaggaa gagggtaatg cacttcagct ccgaagtgtc gtcgccgccc tcggcatcca
     5761 ggcggaaacg ccggtacccg cccagctcgc cgaggtggcg caggttcttt agaccggtct
     5821 cctcgtggat ctcgcgccgg gcggccgcca gcggttcctc gccgggctcc agatgcccct
     5881 tgggcaggga ccaggcccgg ccgcgctggt tgaccaccag cacctggttg cgccggttga
     5941 ggaccacccc gccggcgctg aaggtcttct tcatcggttc agaagggccg gtaatcgcgg
     6001 tcggtgccga agaagtcgtc gtggtagaag tcgatggtcg gcaggatgtc ctgccccgac
     6061 tcctccagcc gggccagcaa ctcccggtag cgggcgaggt agcggccgaa agtctcgttt
     6121 tcgtggcgca tcggctccag cacgaagggc gcgtggtagg cggcccgccg gccggcgatc
     6181 tcgtccatca gcgcccggtg tccggccagc gcctcttcca gccggccggc ctggcgggcc
     6241 atctcggcca ggcaggtccg ccgttcggcc ctgccctggc gcaggtagcc gtagtggcgg
     6301 ttgaagtcca tggcgtggta agccagccgg acggtggtca gcagccggcg caggtcctgg
     6361 ccggcgcggt cggccaccgc ggcccggtaa tcgccctccg gcagtccggc gatcagccgc
     6421 tgcagttccc ggtcggcctt ctcgccggcg gcgatgaagg cccgccactg ggccggcgtg
     6481 agcgggcagc ggtcggcgaa ggccgagacc acctggccga cccgggccgg cggccgggcc
     6541 ccgtagcgga aggaagcgtg gatcggttcg gtcagggcgc cgaaggtgtc caggaggcgc
     6601 cggtagacat cgcggatctt ttcgtaacgg gcggcccggc ggccgaagag gaaggcgacg
     6661 aaccggtcgt cgaactcctt cacgccggcc ccctcctggt tccagctgaa ctcggagagg
     6721 cagcagtact ggtcccagcg gtgcgggtcg tcgtgcgaat agctcagcac cccgtaggag
     6781 ccggcccgtt tgcccaggct gttcagccgg gccaggctgt cggtgtggct ggagaccggc
     6841 ggcagctccc gttccggggt caggtggttg aggcagaggc tcgggttgac gagcgaggcc
     6901 agccgccggt ccaccttcca gaagaagagc cacttccggg gcgagaggtc cggcgcccgg
     6961 tccgggttct cgtagcacca ccagccgacc gccaggtggc gccgcagccc ctccttctcc
     7021 aggatctcca tcagccggtc gttgaagttg ccctgccggt agaggatgtc gtaccagaag
     7081 atgatccgct tgatcccgcg gctcttcagg tggccggcgc agcggaccag gtgctcgatg
     7141 aagaagtccg ccatgccccg gcccggccgc ttctggcact ccggacagcg gcagcgggcg
     7201 tagacctcgt ccaggccgag gccgaaatcg gttatcccgt acggcttcaa ggtctccagt
     7261 atctcgtcgt agagggcgaa gagcagctcg taggttttcc ggttttcggt gcagaagccg
     7321 tagccggtcg gccggccggc ctcgtccttc attgagacct ccgggaaggc cttcggcagg
     7381 taggtgttgt ggcccagcga actgaagtag gggatgaaat tcaggcccag ctcccgggcg
     7441 tagcgaacca gctcggccag gaagtcctcc tcgtacatcc ggggataatg tttccgcacc
     7501 cggccctcgg cgtggtcgta gtactggtta agggtcttca gttccgggaa cttgcgggag
     7561 cggaagagga ggaactccag gtccgcgctc cgccgccagc agttgtaaag cgagaccgag
     7621 acgctgttca tcttcagcag gtagagtttc tccagcgccc gcttccagtc ctccagcccc
     7681 atcaggttgg tgccgaagat cgactcgtag aaggggccgc gctcgcggaa gtccggccag
     7741 tcgaccacct cgaccggcgg cagcagggtc cggccgtttt cacgccggaa gagctgggcc
     7801 agggtcttga ccccgttggc caggcccgtc tccgactccg agacgaccag gatccggtgg
     7861 cggcgggact ggacctcgag ccggtagccc tgttcgcgct ggtagcgctc gaggcgttcg
     7921 gccgggagct tctccgtggt ccaggcctcc cggcgcgtct ccaggcgcca ggcagcggcg
     7981 gccggatggc cggcggccag ttccggcacg gatgaaccgt ggcggccggc gatctcgaag
     8041 acgcgggcca cctccttcgg ctttatcccg ccggggagca cgaagaggcg gttgaccggg
     8101 gtcaaacggc ccggccgccg caggatggac ttcggtttgg gaaagaccag caccgcttct
     8161 cgcacggaca tgttttttct ccgcggtaag ttttaaggtt tgcggcctga cggcgctttt
     8221 ggctggaatt acccactatt atagaccgga aaaggggttt ctgcaactgg ggccgccccc
     8281 gggccggccg gaacggtcaa aaaggcgcgg cggccgcgcc ggcccgtgct ataatacaac
     8341 ggggaagccg tccatccggg cggctcccgg ggagaaagat gaaaaggggc aatccgaata
     8401 cctggctgaa ggcccgggcc gccgaagaga ttgaatggta cctcagttcg gccgacgtga
     8461 tcgtggtcga gcggaagcgg tcgatccggc tgctggggga gctctttgcc caccactttt
     8521 cagagccggc cggcctccgc gtgctcgacc tgggcggcgg cgacggcctc gtgaccgaat
     8581 acttttccaa ccagtacccc cggaacgact tctgcctcat ggacggctcg gtcgcgatgc
     8641 tcgaccaggc aaaggcgcgc ctgaaggggc ggccgatccg gttcgtcgag aagagtttcg
     8701 aggcttacgc ccaaaagggg ctcgatcctg acgggtacga cttcatcttc tcctccatgg
     8761 ccatccacca cctgcccctg aaggagaagg gcgccctcta cgcgcgggtc caccgggagc
     8821 tgcggggaaa cggtcttttc ctggtcttcg atgtcgtggc gccggtctcc ccgaaatcgg
     8881 agcggctcca gttccggatg tgggtggact ggatgaacga agccctgcgg aggagcaact
     8941 ttgccgggga ggtcgggaag tttgacggtt ttccggccgt ttacaaggcc aaggcggaga
     9001 accatccgag ccgcctgctg gaccagctgg accagctgaa gaaggcgggc ttcaagaatg
     9061 tcgactgctt ctt
//
LOCUS       DAOQYN010000059         9064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8172_length_9064_cov_4.740593, whole genome shotgun sequence.
ACCESSION   DAOQYN010000059 DAOQYN010000000
VERSION     DAOQYN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8172_length_9064_cov_4.740593"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PKN80_03565"
     CDS             complement(<1..736)
                     /locus_tag="PKN80_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNQ35124.1"
                     /translation="MVSGRVLTGFGARLLAGRGVPPANARYVAESAVLNEAAGVTTHG
                     VVQFFYFDKQVGPVIDPSAEPKLVLDRSAVALVDGNGSFAQLAMRLAARIAAGKARRF
                     GIGLVCVRNTSWLAGLGVKLLPLVRQGFFAELWAGSAFKYVAPFGGCEARLGTNPIAF
                     GFPVPGRPPVICDFSTSTVARGRAGLMIHAGDRAPEKIFLDGRGRATDNSAVLEAGGS
                     IMLLGGEHYGYKGFGLSLWVEALGLMA"
     gene            complement(744..1463)
                     /locus_tag="PKN80_03570"
     CDS             complement(744..1463)
                     /locus_tag="PKN80_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNQ35125.1"
                     /translation="MKQMRVTCHDGLFRLEAIPFRIAAARAAEKLNIAGDGEDFESLE
                     RLIAEAEAAGAPRAAYRVSYLDERHDEEVIIDRQRFRSRVLSVNLEKAHRVFPYVVTC
                     GPELEDWSKRQSDPLFAYFADYIKQAALAASRKFAFGYIKETYRLSKFSTMAPGSLAD
                     WPIQEQRPLFDLLGPVRETTGVVLTESFLMLPTKSVSGILFPTGSSFASCMLCPREHC
                     VGRSAPYDEKLYAEKYRKQAG"
     gene            1694..2278
                     /locus_tag="PKN80_03575"
     CDS             1694..2278
                     /locus_tag="PKN80_03575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNQ35126.1"
                     /translation="MEEAPKSGKYALVIIDMQNDFVLPGAPAGVAGAYATIPQIRKLL
                     DFFRTRGWPVFHAVREYRADGSDIEITRLDEFLKGRRYVVPGTPGCEIVAELAPVEGE
                     YRIVKNRFSAFMKTELDFMLRRLGVTRLAVCGTQYPNCIRATIFDAICYGYQVTSVTD
                     ATSAQTPEVAAANIRDLENVGVACLRLEEFLDSL"
     gene            2603..4171
                     /locus_tag="PKN80_03580"
     CDS             2603..4171
                     /locus_tag="PKN80_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNQ35127.1"
                     /translation="MIKTTTRGLAALLMLAVACFTAVPGLTEETDRTPGPPGFSPQTG
                     PAAAAATDSAKIDTPLEITIGDAILTALQNNRALRVERLNPAIRETYEQEEAARFDPA
                     LAAELSAARERVKAESRTTGTIADTSEGRTGAGLSLSRFLPTGTEFSVGLTTDLDWSE
                     LYSDQYATRLGLTVTQSLLRGAGREFNLASVRQARLDTLSSRHELRGFTESLVAEVET
                     TYWNYALARLQIQIYQKSADLAEQQLKEVEDRIEVGKLAEIELAAAQAELALRKEALI
                     NAQSNLDALRLRLARLLNPGRGGAWNGPIILKNQPAVPEVKLDDLESHCAVALRLRPD
                     LNQARLEVLRGELEIVKTKNGLLPRMDLFINLGKSGYAGSFGGSLEDLGGRSYDISAG
                     ISLELPPGNRAAESRHRRAVLNHDQAGEALENLAQLAELDVRLAYLEVNRAAQQIKAT
                     VATRRFQEEKLRAETEKFRVGRSTPLMVAQAQRDLVDSQISEIKAIVNHLNSLVELFR
                     VEGSLLERRGISTD"
     gene            4210..5445
                     /locus_tag="PKN80_03585"
     CDS             4210..5445
                     /locus_tag="PKN80_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNQ35128.1"
                     /translation="MKRVVLVIVALALLGLFGWQAYRKISKKMKAPGGRPDRMAVAVE
                     VAPVRQTAISDTRQFSGSLAPRSQFVVAPKVAGRLEKLLVDLGDDVKRNQLIAALDSQ
                     EYEQAVEEARAELKVARANIAESESALEVAKRELERTRALREKKIAPEAELDEAEAAY
                     KTREARHKVTLAQVEQKEAALKAAEVRLSFTQIRAAWEGGSEVRRIGERYVDQGVMLR
                     ANDPIVSILEIDSLTAVIEVTEENYARVRLGQSVRISTDAQPDREFTATIVRISPALK
                     ESSRTARVEALINNQKHLLKPGLFIRAEIEVAHREGATVVPLAALTTRDEKSGVFLLE
                     PDGKKVHFVPVETGIVNSESAEILAPKLEGRVVTLGHHLLEDGSPVLLPESDPGRQPA
                     GQTRTGAGAEKKPTVETRP"
     gene            5442..8531
                     /locus_tag="PKN80_03590"
     CDS             5442..8531
                     /locus_tag="PKN80_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNQ35129.1"
                     /translation="MKISQFTVHRPKLTTMMVLIVIIIGGVSMRRLPVDLMPDISYPT
                     LTISATYENASPEEVEELVTRPIEEAMSAVPGVEEISSESAEGSSRVRVSFAWGTNLD
                     NAASDVRDRLDRVINRLPEEMDRPTLRKFDPSSMPILVIGASSNLDPIQVRRIIDEQI
                     KYRIERVPGVAALDVMGGREREIRINLNADRVKSLGVPLDQVLNSIKAGNITLPSGTV
                     ERGQHEVIIRTPGEYGNLEQLRDTVIADINGVPVRLKDIAAVEDTAQKVTRLVRVNGQ
                     PGVQLSITKQSGKNTVEVAQRVLAEVEKINQDLRQIKLVPIVDSSEYIKRSISSVGSS
                     ALYGGGFAILVLLIFLANIFSTAIIAVAIPISIIAAFALMYFSGFTLNIMTLGGLALG
                     VGMLVDNSIVVLENIYRLREDGLDAETAAVEGSSEVTAAVIASTLTTVVVFLPLIFIQ
                     GMAGVMFTQLALVVSFALLASLAVSLTVVPMLASKYLHPVDPKRLAHETLAHRLLRLN
                     ALWSANLENAYKRILHRALNHQKATLVGVSILLLISLALAPLVGMELMPQSDEGEVRV
                     NGEMAVGTKLSVMDEKFQAIESIILRETPEMKNMVATIGSGGGRMGSGGHVGQFRISL
                     KPRAERKRSSEQVVSDLRRELSNRPGVTIRPRAGTGQFTGMMTRGFGSTERVQVEIRG
                     YDLATADALAEQVKEVVERVDGITDAQLSRESGVPEELVIIDRAKAADLRLSVSQIAQ
                     MLQTSLGGSTAGYYREGGSEYRIFVQLENPEKMEVEDILNLTLTNAAGEPITLRNVVK
                     TQPHEGPVSIQRSDQERIVTVSANTSDRDIGSILRDIRRDIQSVPVPSGFTINFGGEY
                     EQQQESFQELGWTFVLALILVYMVMVSLYESLRDPFVVMFSIPPAAIGVVWLLFLTNT
                     TFNMQSIIGCIMLGRIVVNNAIVLVDQINLLRRRDGMALREAIEEAGRRRLRPILMTA
                     LTTMLGLVPMALGLGEGGEFQAPLARAVIGGLFSATLITLVVVPVVYSIFEQRLAKS"
     gene            8579..>9064
                     /locus_tag="PKN80_03595"
     CDS             8579..>9064
                     /locus_tag="PKN80_03595"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HNQ35130.1"
                     /translation="MTHQKPLNEEEKRVIIGRGTEPPFRNRYWNHFKKGIYRCRNCGA
                     PLYRSENKFDSGCGWPAFDDEIPGAVARRPDADGRRTEITCAGCGGHLGHVFSGEGLT
                     PRNIRHCVNSVSLLFEPAGTKIERAVFAGGCFWGIEYHFWKAAGVVSVASGYTGGRLE
                     NP"
BASE COUNT         1867 a         2921 c         2790 g         1486 t
ORIGIN      
        1 cggccatcag cccgagcgcc tccacccaga gcgaaagacc gaatcccttg taaccatagt
       61 gctcgccgcc caggagcatg atgctcccgc ccgcttccag aacggccgaa ttgtcagtag
      121 cccggccgcg gccatcgagg aagatcttct ccggcgcccg gtctccggca tgaatcataa
      181 ggccggcccg gccccgggcc acggtggagg tggagaagtc gcatatcacc gggggacggc
      241 cgggcaccgg gaaaccgaaa gcgatcgggt tggtccccag ccgggcctcg cagccgccga
      301 agggcgccac gtacttaaag gcggaaccgg cccacagctc ggcgaagaat ccctgccgca
      361 ccagcgggag gagcttgacg cccaggccgg ccagccagga ggtgttccgg acgcagacca
      421 ggccgatccc gaacctccgg gccttgccgg ccgcgatccg ggcggccagg cgcatggcca
      481 actgggcaaa cgacccgttg ccgtccacca gggctacggc cgaccgatcc agcaccagct
      541 tcggctcggc cgaaggatcg atgaccggcc cgacctgctt gtcgaaataa aaaaactgga
      601 ccaccccgtg ggtggtgacg ccggccgctt cgttcaggac ggccgattcg gccacgtaac
      661 gggcgttggc cgggggtacg ccgcggccgg ccagcaggcg ggctccgaaa ccggtcagga
      721 cccgaccgga aaccaagccg ccctcagccg gcttgcttcc ggtacttttc cgcatagagc
      781 ttctcatcgt aaggggccga ccggccgacg caatgctccc gggggcagag catacaactg
      841 gcgaaactgc ttccggtcgg aaagaggata ccggagaccg acttggtcgg gagcatcagg
      901 aaactctcgg tcaacacgac gccggtcgtt tcccggaccg gtccgagaag gtcgaagagg
      961 ggccgctgtt cctggatcgg ccagtcggcc agggagccgg gggccatcgt ggaaaacttc
     1021 gaaagccggt aggtctcctt gatataaccg aaggcaaact tgcgggacgc ggccagggcg
     1081 gcctgcttga tgtagtcggc aaagtaggcg aagagcgggt cggattgccg cttcgaccaa
     1141 tcctcgagct ccgggccgca ggtgacgacg taggggaaga cccggtgggc cttttcgagg
     1201 ttgacgctga gcacccggct ccggaaccgt tgccggtcaa tgatcacctc ctcgtcgtgc
     1261 ctttcgtcca ggtaggaaac ccggtaagcg gcccggggcg cgcccgcggc ttccgcctcg
     1321 gcgatcaacc tctccaacga ttcgaaatcc tccccgtccc cggcgatatt aagtttttcg
     1381 gccgcccggg cggcggcgat acgaaaggga atcgcctcca gccggaagag gccgtcatgg
     1441 caggttactc gcatttgttt catgaatttc tccggttttg ccatcacatc tatccttctg
     1501 ctcctgagaa taaaaagcag tataatccct ccttcaagga atgtcaacga tttccagacg
     1561 gtcaaaaccg gagattgcca ataaaagccg cccggctata tactatcagg aaaccggaaa
     1621 ggccttgccg gcggaaattc aaccggaatg caccgctgcc ggccagaacc agcgatcaaa
     1681 aagagagttc ggcatggaag aagccccaaa aagcgggaaa tacgccctgg tcatcatcga
     1741 catgcagaac gatttcgtcc tgcccggggc gccggccggc gtcgccggcg cctacgccac
     1801 cataccccaa atcagaaaac tgctcgattt tttccggacg cgaggttggc cggtcttcca
     1861 tgccgtccgc gagtaccggg ccgacggcag cgacattgaa atcacccggc ttgatgaatt
     1921 cctgaagggg cgtcgttacg tggtgccggg caccccggga tgcgaaattg tcgcggaact
     1981 ggccccggtc gaaggggagt accggatcgt caagaaccgg ttcagcgcct tcatgaaaac
     2041 ggaactggac ttcatgctga gacggctcgg ggtcacccgc ctggccgtct gcggtaccca
     2101 gtaccccaac tgcatccggg ccaccatttt tgacgccatc tgctacgggt accaggttac
     2161 cagcgtgacc gacgccacct cggcccagac gccggaggtc gccgcggcca acatcaggga
     2221 ccttgagaat gtcggagtgg cctgcctccg actggaggaa ttcctggact cgttatgagg
     2281 cggcttcaaa ccatccaaag cgtgattatc ttggtcaggc cgcgaccgcg agccagatat
     2341 ttaaacgaat agggaggcgg gcagctctta taaaatacag gcttcccgcc ttccgtagtt
     2401 ctggcggccg gagtcgggcc ggtttacatt ctttcgataa aatgtcaaaa taattaaaat
     2461 aaaccggcct tttaaacaat atttacaaat cgtctcctgc cggcgggtta cgatcattcc
     2521 gaactgaagc ccgggcatcc tttttgaccg ggccgccggg aagccgagtc caaacataaa
     2581 aaagaagcaa cctaaaaccg gattgatcaa aaccacgacc cgggggctgg ccgccctcct
     2641 gatgctagcc gtggcctgtt tcacggcggt cccgggcctc accgaggaga cggaccggac
     2701 acccggaccg cccggtttct ctccccagac cggcccggcc gcggccgccg caaccgactc
     2761 ggccaaaatt gataccccgc ttgaaataac gatcggcgac gccatcctga cagcactcca
     2821 gaataaccgg gcgctccggg tcgaacgcct caacccggcc atccgtgaaa cctacgagca
     2881 ggaagaggcg gccaggttcg atcccgcact ggccgccgaa ctctcggccg cccgggaacg
     2941 ggtcaaggcc gaatcgcgga caacgggcac gatcgccgat acctcggagg gtcggaccgg
     3001 cgccggcctt tcgctctcca gattcctacc caccggcacc gagttctcag tcggcctgac
     3061 aaccgacctc gactggtccg aactctacag cgaccaatat gccacccggc tcgggctgac
     3121 ggtaacccag tcactgctcc gcggcgccgg gcgggaattc aacctcgcca gcgttcgcca
     3181 ggccaggctg gacacgctct cctcccggca tgaactgcgc ggcttcaccg agagcctggt
     3241 cgccgaagtc gagaccactt actggaacta cgcgctcgcc cggctccaga tccaaatcta
     3301 ccagaaatcc gccgacctgg ccgaacagca gttgaaggag gtcgaggaca ggatcgaggt
     3361 ggggaaactg gccgagatcg aactggccgc ggcccaggcc gagctggcgc tgcgaaaaga
     3421 agcgctgata aacgcgcaga gcaacctcga cgccctgcgg ttgcgcctgg cccggcttct
     3481 caaccccggc cggggcggcg cctggaacgg gccgatcatt ctgaagaacc agccagccgt
     3541 gccggaggtg aaactggacg acctggaatc gcattgcgcc gtggcgctgc gcctgcgtcc
     3601 cgatctcaac caggcccgcc tggaggtgct ccggggggaa ctggagatag tgaagaccaa
     3661 aaacgggctg ctgcccagaa tggacctgtt tatcaacctc gggaaaagcg gatacgccgg
     3721 ctcattcggc ggttcgctcg aggatctcgg cggacggagt tacgatatct cggccggaat
     3781 aagcctggaa ttgccgccgg gtaaccgtgc ggcggaatct cgccaccggc gggcggtcct
     3841 caatcacgac caggccggag aagcgctgga aaaccttgcc cagctggccg aactggacgt
     3901 ccgcctggcc tacctcgagg tgaaccgggc cgcccagcag atcaaggcaa ccgtcgccac
     3961 gcgacgcttc caggaggaaa aactacgggc cgaaaccgaa aagttccggg tcggccgttc
     4021 gacgccgctg atggtcgccc aggcccagcg ggacctggtc gacagccaga tatccgaaat
     4081 caaggcgatc gtcaatcacc tcaattcgct ggtcgaactc ttccgcgtcg aaggctcact
     4141 gctcgaacgg cgcggaatct cgaccgatta aataacaggg acggatagaa taaccactgg
     4201 agcttaccga tgaaaagagt cgtcctggta atcgtggccc tggccctgct gggcctcttc
     4261 ggctggcagg cctaccgaaa gatcagtaag aaaatgaagg ccccgggcgg ccgtccggac
     4321 cgaatggccg tcgctgtcga agtggctccg gtgcggcaaa ccgccatcag cgacacgaga
     4381 cagttcagcg gttcgctggc gcccaggtcc cagtttgtcg tggctccgaa ggtcgccggc
     4441 cgcctggaga aactcctggt cgacctgggc gacgatgtca agcggaacca gctgatcgcc
     4501 gcgctggaca gccaggagta cgaacaggcg gtcgaagagg cccgggcgga gctgaaggtg
     4561 gcccgggcca atatcgccga atcggagagc gccctggagg tggcgaagcg cgaactggaa
     4621 agaacccggg cgctccggga gaagaagatc gcccccgaag ccgagctcga cgaggccgaa
     4681 gccgcttaca agacccggga ggccaggcac aaggtgacgc tggcccaggt ggagcagaag
     4741 gaggccgccc tgaaggcggc cgaggtccgg ctctccttca cccagatccg ggccgcctgg
     4801 gagggcggca gcgaggtccg ccgaataggc gaacgctacg tggaccaggg agtcatgctc
     4861 cgggccaacg acccgatcgt ttcgatcctg gagattgatt cgctgacggc ggtcatcgag
     4921 gtgaccgagg aaaattacgc ccgggtccgg ctgggacagt cggtgcggat aagcaccgac
     4981 gcccagcccg accgtgagtt cacggccacc atcgtccgca tttcgcccgc gctcaaggaa
     5041 tcctctcgta ccgcccgggt cgaggcgctg atcaacaacc agaagcacct gctcaagccc
     5101 gggctcttca tccgggccga aatcgaggtg gcccaccggg aaggcgccac ggtggtcccc
     5161 ttggccgccc tgaccacccg ggacgaaaaa tcgggggttt tccttctcga acccgacggc
     5221 aagaaggtcc atttcgtccc ggtggaaacg ggcatcgtca actcggaatc ggccgaaatc
     5281 ctcgcgccga agctggaagg acgcgtcgta accctcggcc atcacctgct cgaagacggg
     5341 tctccggtgc tcctgcccga atccgacccg ggtcggcaac cggccgggca aacccggacc
     5401 ggcgccggtg ccgagaagaa gccgaccgtg gaaacccgtc catgaagata tcccagttta
     5461 cggtccaccg gcccaagctg accacgatga tggtgctgat cgtcatcatc atcggcggcg
     5521 tctcgatgcg ccggctgccc gttgacctga tgccggacat cagctaccca accctcacca
     5581 tctcggccac ctacgagaac gccagcccgg aagaggtcga ggaactggtc acccggccga
     5641 ttgaagaggc catgagcgcc gtcccggggg tcgaggagat ctcgtcagaa tcggccgagg
     5701 gcagcagccg ggtgcgggtc tcgtttgcct gggggacgaa cctggacaac gcggccagcg
     5761 acgtgcggga ccgcctcgac cgcgtgatca accggctgcc ggaggagatg gaccggccga
     5821 cgctgaggaa attcgaccca tccagcatgc cgatcctggt catcggcgcc tccagcaacc
     5881 tggatccgat ccaggtccgc cggatcattg acgaacagat caagtaccgc atcgagcgcg
     5941 tgcccggggt ggccgctctg gacgtgatgg gcggccggga acgggagatc cggatcaatt
     6001 tgaacgcgga ccgggtcaag tcgcttggag tcccgctgga ccaggtgctg aactccatca
     6061 aggccgggaa cataaccctg ccctcgggaa ccgtcgaacg gggccagcac gaggttatca
     6121 tccggacccc gggcgaatac ggcaatctgg aacagcttcg ggataccgtg atcgccgaca
     6181 tcaacggcgt gccggtccgg ctgaaggaca tcgccgcggt cgaggatacc gcccagaagg
     6241 tcacccgcct ggtccgggtc aatggccagc cgggcgtcca gctctccata accaagcagt
     6301 ccggcaagaa taccgtcgag gtggctcaaa gggtcctggc cgaggttgaa aagatcaacc
     6361 aggatctccg gcagattaaa ctcgtcccca tcgtcgactc atctgagtac atcaagcgtt
     6421 ccatctcctc ggtcggttcc tcggccctct acggcggcgg cttcgccatc ctcgtcctgc
     6481 tcattttcct tgccaatatc ttcagcaccg ccatcatcgc cgtcgccatc ccgatctcga
     6541 ttatcgccgc cttcgcactg atgtacttct ccggcttcac cctgaacatc atgaccctgg
     6601 gcggcctggc gctgggagtc ggcatgctgg tggataactc gatcgtggtc ctggagaaca
     6661 tctaccgcct gcgggaagac ggcctggatg ccgagacggc cgccgtcgag ggaagcagcg
     6721 aggtcacggc cgccgtcatc gccagcacgc tgaccaccgt ggtggtcttc ctgccgctca
     6781 tcttcatcca ggggatggcc ggggtgatgt tcacccagct ggcgctggtg gttagcttcg
     6841 ccctgctcgc ctccctggcc gtttcgctca cggtggtccc gatgctggcc tccaagtacc
     6901 tgcacccggt cgatccaaaa cggctggccc acgagacgct cgcccaccga ctgctccggt
     6961 tgaacgcgct ctggtccgcc aacctcgaaa acgcctataa aaggatcctg caccgggcgc
     7021 ttaaccatca gaaagccacg ctggtgggcg tatcgatcct gctcctgatc agcctggccc
     7081 tggcccccct ggtcggcatg gaactgatgc cgcagagcga cgagggcgag gtccgcgtca
     7141 acggcgaaat ggcggtgggc accaagctgt cggtcatgga cgaaaagttc caggcgatcg
     7201 agtcgatcat cctgcgggag acccccgaaa tgaaaaacat ggtggccacc atcggcagcg
     7261 gcggcggccg gatgggctcg ggcggccatg tcggccagtt tcgaatctcc cttaaacccc
     7321 gggcggaacg gaaacgctcc agcgagcagg tggtctccga cctgcgccga gaactttcca
     7381 accgacccgg agttaccatc cgaccccggg ccggaaccgg ccagttcacc ggtatgatga
     7441 cacgcgggtt cggcagcacc gaacgggtac aggtggaaat ccgcggctac gacctggcga
     7501 ccgccgatgc gctcgccgaa caggtcaagg aagtggtgga aagggttgat ggaatcaccg
     7561 atgcccagtt aagccgggaa tcgggcgtac cggaggaact cgtcatcata gaccgggcga
     7621 aggcggccga cctgcgcctg tcggtctccc agatcgccca gatgctccag acctcgctgg
     7681 gcggcagcac ggccgggtat taccgggagg gcggcagtga gtaccggatc ttcgtccagc
     7741 tggaaaaccc ggaaaaaatg gaggtggaag atatcctgaa cctcaccctg accaacgcgg
     7801 ccggcgaacc gataaccctg cgcaatgtcg tcaagaccca gccgcacgaa ggaccggtaa
     7861 gcatccagcg ctcggaccag gaacggatcg tgaccgtttc ggccaacacc agcgaccggg
     7921 atataggttc aatcctccgg gacatcaggc gggacatcca gtcggtgccc gtcccaagcg
     7981 ggttcacgat caacttcggc ggcgaatacg aacagcagca ggagagtttc caggaactgg
     8041 gctggacctt tgtcctggcc ctgatcctgg tttacatggt catggtctcg ctttacgaat
     8101 cgctgcgcga cccattcgtg gtcatgttct cgatcccgcc ggcggccatc ggcgttgtct
     8161 ggctgctctt tttaaccaac acgaccttca acatgcagtc aatcatcgga tgcatcatgc
     8221 tcggcaggat cgtggttaac aatgccatcg tcctggtgga ccagatcaac ctgctgcgcc
     8281 gccgcgacgg gatggccctg cgcgaagcca ttgaagaggc cggccggcgg cgcctgcggc
     8341 caatcctgat gacggccttg acgaccatgc tcggcctggt gccgatggcc ctggggcttg
     8401 gcgagggcgg ggaattccag gcgcctctgg cccgggcggt catcggtggc ctcttcagcg
     8461 ccaccctgat cacgctggtc gtcgtgccgg tcgtttactc gatctttgaa cagcgcctgg
     8521 ctaaaagttg atctttttct cttttcgacg cgtccccccc tgaatcaagc ggaaccaaat
     8581 gacgcatcaa aaacccctga acgaagaaga gaagcgggtc atcatcggcc ggggtaccga
     8641 accgcccttt cgaaaccgtt actggaatca tttcaaaaag ggtatctacc gctgccgcaa
     8701 ttgcggcgcc ccactctacc gttcggaaaa caagttcgat tccggctgcg gctggcccgc
     8761 cttcgacgac gaaataccgg gggcggtcgc ccgccggccc gatgccgacg gccggcggac
     8821 cgagataacc tgtgccggct gcggcggcca cctgggtcac gtcttttcag gcgaggggct
     8881 gacacccagg aatatccgcc actgcgtcaa ctcggtctcc ctgctcttcg aaccggccgg
     8941 aacgaaaatc gaacgggcgg tcttcgccgg aggatgcttc tggggcattg agtaccattt
     9001 ctggaaagcg gccggggttg tctcggtcgc ctcgggatac acggggggcc ggctcgaaaa
     9061 cccg
//
LOCUS       DAOQYN010000060         9042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8194_length_9042_cov_4.337487, whole genome shotgun sequence.
ACCESSION   DAOQYN010000060 DAOQYN010000000
VERSION     DAOQYN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8194_length_9042_cov_4.337487"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PKN80_03600"
     CDS             complement(<1..618)
                     /locus_tag="PKN80_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35131.1"
                     /translation="MPIHQDMKEPAYEVSFAGARGRRIEVTAGAASVWKFRFACGSRP
                     VKAGGGLKIFCEVPKFWLGLIPQKDDPDRKWFCRAAGSVPAEIVELAGSYKSLCLAGI
                     RLPEGLSEGQSVEFSFGTPERPAYAIAHAYNRVLVSMMVDYEADGGFTRIWPPLRIDV
                     IPGPAVKAAVVLPSVVRPGQPLVIRARFEDVNSNPVEAAGRPVRLL"
     gene            complement(876..3584)
                     /locus_tag="PKN80_03605"
     CDS             complement(876..3584)
                     /locus_tag="PKN80_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNQ35132.1"
                     /translation="MNNQWYRMTEKAVIEQLSSAFNGLSAAEAERRISEYGYNQLEMK
                     KEETLFSIFIRQFKSPLIYILLFAALISFAVKKNTNGIVIFTVLMVNALTGFIQESRA
                     RKSMEALKKLSAPRTRVLRDGKTVEVATKNLVPGDVILLEAGTKIPADARVIKSVRLQ
                     VNESALTGESHPVYKMTGPIEDEVGVPEMLNMVFSGTVTVSGRGTAVVVETGPSTQIG
                     QMAEIIQSAPESKTPFQRKVEKLGKFIILLVSLQISIVFLVGFLVRRLPFYDIFMVVL
                     SQLVSSIPEGLPVALTVALAVGMQRMAKRKALIRKLYAVEGIGCVSVICSDKTGTLTR
                     NEMTSKKLATVDTDEVEITGVGYLREGEVLIKGARPDLGREARDVEKLLTIGVLCNNA
                     EAAVSGDNKELDVDGDPTEIAYLVAALKAGFDLEVLRKKYPRIDELPFDPDIRLMATV
                     HNTPAGDHLVCVKGAPEKLVQLCGSYLASDGALKEMDAEGRAAIFRKAESLADSALRV
                     LAFGYCSRAMEDLRHLEFDSLRGCLVFVGLIGNIDPPRLEVKKAIEDCRRAGIRTIMV
                     TGDHLSTARAIGAELGIQGKSDPGLEGKDISRMSDEELAESLKTTSVFARIEPRHKFK
                     IVQVLQKYGATVAMTGDGLNDAPALSAADIGVAMGITGTDIAKDASDIVIADDNFATI
                     VNAVEEGRGITANMRKTLIYLLTSSNTEVLVLLTTMLVGLPLPLLPLMILWINLLTDG
                     AQTVNLIMEPKEDVMSQPPDPKEEPLLTPQIWKRLLVRVPVMATGIFGLFFYELKIGT
                     TLAYARTVAFTTLACSQWMNGLSSRSLTRSVFRMSLLSNKYLLYGLSVAVSLQLLVVY
                     NPFFQRVFETQALKLIDWMKIIAASTAVLWAEEIKKYYRRRQAART"
     gene            4015..5391
                     /locus_tag="PKN80_03610"
     CDS             4015..5391
                     /locus_tag="PKN80_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNQ35133.1"
                     /translation="MSVLVEKKVVRTLFAMALPMLAGTFAMNAYHLTDTWFVSRLGTL
                     ALAAMGFTFPVVMLLVCVARGIGSGLTTLVSHAIGRHDHEEASRLVTHGIMLTLAVTA
                     VMSIGGYLLITPIFKSLGAGAAALPLVSAYMRTWFIGAPTMTLPMVGSGILISAGDSK
                     AASWLMIVGTLVNLVLDPIMIFGFLGCPAMGIRGAALATVISQAISMLWLLRLLYKKH
                     RFLAWRRWTAAEYGLSLRRITGFAVPSVLSMLLMPISATVITKLLSGFGYEAVAASGA
                     AGRIEMFAFMIPMALGISLTPFISQNFGAKRLDRIQEAKKWSTGFALAYGGLMTAAFF
                     IFAPHLAAIFTKDPKVAETIIAYIRITCFGYGMMEVHRYCGFFLTGLHRPVAATLLNG
                     VRVLVFLIPLSYLGAHLIGLRGVFWGRLVTDLSVGAIGFAWASHALRKANSSNIKEDG
                     WTPMSFGE"
     gene            5500..6873
                     /locus_tag="PKN80_03615"
     CDS             5500..6873
                     /locus_tag="PKN80_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNQ35134.1"
                     /translation="MDKTTRHPTESILESISDGVFTVDSEWRITSFNRAAEKITGVPR
                     REAVGRRCSEVFRCSMCEASCALQKTLKTGKPIIGKSGYIINAEGNRIPISVSTAVLK
                     DNRGRTIGGAETFRDLSEVEALRRELEGKAQVGNLTSRSPLMQRIFEVLPAIAASPST
                     VLILGETGTGKELVARTVHDLSPRRDSPFLAVNCGALPDTLLESELFGYKAGAFTDAR
                     KDKPGRFALAGGGTLFLDEIGEISPALQVRLLRVLQERTYEPLGATGSETTDARIIVA
                     TNKNLDEATRRGTFREDLYYRVNVVRIELPPLRRRKEDIPLLAEQFIARFNRLQRKSV
                     TGLSSEALSLLMGHDWPGNIRELENIIERAFILCPEGQVGIEHLPAELTGRDASVHSK
                     NRLRLSRDVLEAQAIREALEKNRFNRLAAARALGVHKSTLFRKIDRLGIKVPKADRKD
                     SRGIKSH"
     gene            7059..7529
                     /locus_tag="PKN80_03620"
     CDS             7059..7529
                     /locus_tag="PKN80_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNQ35135.1"
                     /translation="MKIALSIWNERIAPVFDVSSRISLIEVDGERVTWKSAEELPGAA
                     SFAAAAWLAEQGVETLICGAVSRPVLARLTGYGIRVIPFVAGGRREVVEAWLNGTLER
                     KPFLMPGCRRGGPGPGRRGCRGRGHGGMASGCLWQETAAARIRTKEFKPSQKGG"
     gene            7624..7773
                     /locus_tag="PKN80_03625"
     CDS             7624..7773
                     /locus_tag="PKN80_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35136.1"
                     /translation="MGRRGGPLAAGPGGYCVCVKCGERLPHERGVPCVEHKCPKCGGA
                     MTREV"
     gene            7801..8166
                     /locus_tag="PKN80_03630"
     CDS             7801..8166
                     /locus_tag="PKN80_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNQ35137.1"
                     /translation="MPRGDGTGPMGQGPMSGRAAGYCAGYAVPGYANQIPGRGLGRGA
                     GRGYAAWGRGCFGGGRGWRNWYHATGLPGWMRYNYAAPAADPGLEKQALQNQVEILES
                     QLDLVKKRISELEKAPEKE"
     gene            8278..8640
                     /locus_tag="PKN80_03635"
     CDS             8278..8640
                     /locus_tag="PKN80_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNQ35138.1"
                     /translation="MKIVFTTSGDNLQAELDARFGRAPKFLVYDLEKEHYLVVDNQQN
                     LQAAQGAGIQSAETVAKLGAGAVVTGHCGPKAFRVLKAAGIKIYNTDAATVAEALNRY
                     RAGELTPAESADVEGHWS"
     gene            8637..>9042
                     /gene="tsaA"
                     /locus_tag="PKN80_03640"
     CDS             8637..>9042
                     /gene="tsaA"
                     /locus_tag="PKN80_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNQ35139.1"
                     /translation="MNLEIITYRPIGIIRSPHQKPEETPIQPAYARGCPGRAEVFPEF
                     ADGLRDLEGFSHLYLIFHLHRAGPAKLLVKPFLQDVDRGVFATRSPRRPNPIGLSIVR
                     LVGREGSVLLLKDLDILDGTPLLDIKPYTAKFD"
BASE COUNT         1807 a         2890 c         2715 g         1630 t
ORIGIN      
        1 cagcaggcgc accggccggc cggccgcctc gaccgggttg gagttgacat cctcgaaccg
       61 ggcccggata accagcggct ggccgggccg gacgaccgag ggcagcacga ccgccgcctt
      121 gaccgccggg ccgggaatga cgtcgatgcg caggggcggc cagatcctcg taaagccgcc
      181 gtcggcctcg taatcaacca tcatcgaaac caggacccgg ttataggcat gggcgatggc
      241 gtaggcgggc cgctccggcg tcccgaagct gaactccacc gattggccct cggagaggcc
      301 ctcgggcagg cggatcccgg ccaggcagag cgacttgtag gaaccggcca gctccacgat
      361 ctcggccgga accgaaccgg ccgcccggca gaaccatttc cggtccgggt catccttctg
      421 cgggatgagt ccgagccaga acttgggcac ctcgcagaaa atcttgagcc cgccgccggc
      481 cttgaccggc cggctcccgc aggcaaaccg gaacttccag accgaggcgg ccccggcggt
      541 tacttcgatc cggcggcccc gggcgccggc aaacgaaact tcgtaggcgg gttccttcat
      601 gtcttgatgg atgggcatta atcttctccg gtgatttctc tcgataagga ttagatacca
      661 agcgggctgt ccgtgaagaa ctccgttcaa atcttatcat cagggccaag ccgggtcaag
      721 gcaagaggag ggggcggaat atgaatttca gcctatgcta aaaaaacggc tccggcggac
      781 atatccgcct tttaagcaag gaggtggcgg gcaagggaag gggggccgca gcggacaaaa
      841 agaggccggc cgcccccgcg caataattca aaaagtcaag tcctggcagc ctgtcggcgg
      901 cgatagtact tcttgatctc ctcggcccag agcaccgcgg tgctggccgc gatgatcttc
      961 atccagtcga tcaatttcag ggcctgggtt tcgaagaccc tctggaaaaa cgggttgtaa
     1021 actacgagca gctgcaggga gactgcgacc gaaagaccgt aaagaaggta cttattgctc
     1081 aacagggaca ttcgaaagac cgagcgggtc agggatcggc tgctgaggcc gttcatccac
     1141 tggctgcagg ccagcgtggt gaaggcgacc gtccgggcgt aagccagcgt ggtcccgatc
     1201 ttcaattcat aaaaaaagag gccgaagatg cccgtcgcca tcaccggtac ccgcaccagg
     1261 agccgtttcc agatctgcgg agtcagcaac ggctcttcct tggggtcggg gggctgggac
     1321 atcacgtcct ctttcggctc catgatgaga ttgaccgtct gggccccgtc ggtgagcagg
     1381 ttgatccaga ggatcatgag cggcaacagg ggcagaggca ggccgaccag catggtggtc
     1441 agcaggacca gcacttcggt attgctcgag gtcaggaggt aaatcaaggt cttgcgcatg
     1501 ttggcggtga taccccggcc ttcctcgacc gcgttcacga tggtggcgaa gttgtcgtcg
     1561 gcgatcacga tatcgctggc gtccttggca atgtcggtac cggtaatccc catcgccact
     1621 ccaatatcgg ccgcggagag agccggcgcg tcgttcagtc cgtcgccggt catcgccacg
     1681 gtcgcgccat acttctgcaa tacctgcacg atcttgaact tgtgccgggg ctcgatccgc
     1741 gcgaagaccg aggtcgtctt caaactctcg gcgagttcct cgtcgctcat ccggctgatg
     1801 tccttgcctt ccaatccggg gtcggatttg ccctggatac ccagttcggc gccgatcgcc
     1861 cgggcagtcg aaaggtgatc gccggtgacc atgatggtcc tgatgccggc gcggcggcaa
     1921 tcctcgatcg ctttctttac ttccaaccgc ggcggatcga tgttgcctat caaaccgaca
     1981 aagacgaggc agccccggag ggagtcgaat tccaggtggc gcagatcctc catggcccgc
     2041 gagcagtagc cgaaggccag cacccgcagg gcgctgtcgg ccagcgactc ggccttcctg
     2101 aagatcgcgg cgcggccctc ggcatccatc tccttcaatg cgccgtcgga ggcaaggtag
     2161 gagccgcaca gctgtaccaa cttttcgggg gcgcctttta cgcaaaccag gtgatcaccg
     2221 gccggggtat tgtgcaccgt cgccatcagg cggatatccg gatcaaaggg cagctcgtca
     2281 atccgcgggt acttcttgcg taaaacctcg aggtcgaaac cggccttgag ggccgccacc
     2341 aggtaggcga tttcggtggg atccccatct acgtccaatt ccttgttatc accggagacg
     2401 gccgcctcgg cgttgttgca caggacgccg atggtgagta gtttttcaac gtccctggcc
     2461 tcccggccga gatcgggccg cgctcctttg atcaggacct ctccctccct caggtacccg
     2521 acgccggtga tttcaacctc atcggtatcg acggtcgcca gctttttgga ggtcatttcg
     2581 ttgcgggtca gggtaccggt cttgtcgctg caaatgaccg atacgcagcc gatcccctcg
     2641 accgcgtaaa gcttcctgat cagcgccttg cgtttggcca tcctctgcat gcccaccgcc
     2701 agggcgaccg tcagggccac cggcaggccc tcgggaatgg aggagaccag ctggctgagc
     2761 accaccatga aaatgtcgta gaagggaagc cgccggacaa gaaaaccaac caggaaaacg
     2821 atgctgatct ggagcgaaac caggagaatg ataaacttgc ccagtttttc aaccttccgc
     2881 tgaaagggcg tcttggactc cggagccgac tggataatct cggccatctg tcctatctgg
     2941 gtggaggggc cggtttcaac caccaccgcg gtgccccggc cggaaaccgt cacggtgccg
     3001 gaaaaaacca tgttaagcat ttcgggcaca ccgacctcgt cctcgatcgg cccggtcatt
     3061 ttgtaaaccg ggtgcgactc cccggtcagg gccgattcgt ttacctggag gcgcaccgac
     3121 ttgatcaccc gggcgtcagc cggaatcttg gttcccgctt ccaacaggat cacgtcgccg
     3181 ggcaccaggt tcttcgtggc cacctcgact gtcttgccgt cgcgcagtac ccgggtgcgg
     3241 ggcgccgaga gttttttgag ggcctccatc gatttccggg cccggctctc ctggatgaac
     3301 ccggtcagcg cgttgaccat caacaccgtg aaaatgacga tgccgttggt attctttttc
     3361 accgcaaagg aaatcagcgc cgcgaacaac agaatgtaaa tgaggggact cttgaactgc
     3421 cggatgaaaa tcgaaaaaag cgtctcctcc ttcttcatct ccaactggtt ataaccatat
     3481 tcggagatcc gcctttccgc ctccgcggca gaaagtccat tgaaggccga cgacaactgt
     3541 tcaatgaccg ccttttcagt catccggtac cattggttgt tcatggcatc cccttctcag
     3601 cgaacggttg cgtttataaa cgggtggacc ctgacccggg cgaactttca aaaaaagata
     3661 aaaacctaac cactgctact ctggaaatca aagccgctcg gcccgattga attgttcgac
     3721 gtagacaatg ctgagggccg tgtaattata ctggtttcag tggaaaattg caagtcaagc
     3781 gattcgttcc ggcgcctcat tttttactgg cggccggcgg agaagaacga agatatatca
     3841 ttaaatacaa cggctgtctt tttccttttc aggcacgatt taggttatac taaaccatgg
     3901 ctgttgcctg aaccgttcag ccgttccgcc catccgcgaa aacttctgcg gaagccccaa
     3961 aataatagca ggtttgtgtt agccgcatta caaacactaa aaccaaaaac tgacatgtcc
     4021 gtactggttg agaaaaaagt tgtccgcacc ctctttgcga tggccctgcc gatgctggcc
     4081 ggcaccttcg ccatgaatgc ctaccacctg acggacacct ggttcgtctc gcgtctgggc
     4141 acgctcgcgc tggccgccat gggtttcacc ttcccggtcg taatgctgct ggtctgcgtg
     4201 gcgcgcggca tcggttccgg cttaaccacc ctggtctccc acgccatcgg ccgccacgat
     4261 cacgaggagg cctcccggct ggtcacccac ggcatcatgc tcaccctggc ggtcacggcc
     4321 gtcatgtcca tcggcggcta cctgctgata acgccgatct tcaagagcct gggcgccggc
     4381 gcggccgcac tgcccctggt cagcgcctac atgcgcacct ggttcatcgg cgcgccgacc
     4441 atgaccctgc cgatggtcgg cagcggcatc ctgatctcgg ccggagactc caaagcggcc
     4501 agctggctga tgatcgtcgg cactctggtc aacctggtcc tggacccgat catgatcttc
     4561 ggcttcctcg gctgcccggc catgggaatc cggggcgcgg ccctggccac cgtaatctcc
     4621 caggccatct cgatgctctg gctgctccgt ctgttgtata aaaaacaccg cttcctggcc
     4681 tggcggcgct ggaccgccgc cgagtacggg ctctcgttgc ggcgcatcac cggcttcgcc
     4741 gtgcccagcg ttttgagcat gctcctgatg ccgatctccg ccaccgtcat caccaagctg
     4801 ctgagcggat tcggctacga ggcggtggcc gccagcggcg cggccggccg catcgagatg
     4861 ttcgccttca tgatccccat ggcgctgggc atctccctga cgccgttcat cagccagaac
     4921 ttcggcgcca agcggctgga ccgcatccag gaagccaaga agtggtccac cggattcgcg
     4981 ctcgcctacg gcggattaat gacggccgcc ttcttcatct tcgccccaca cttggcggcc
     5041 atcttcacca aggatcccaa agtggccgaa accatcatcg cctacatccg catcacctgc
     5101 ttcggctacg gcatgatgga ggtccaccgt tactgcggct tcttcctcac cgggctgcac
     5161 cggccagtcg ccgccaccct gctcaacggc gtgcgggtcc tggtcttcct gatcccgctc
     5221 tcctacctgg gcgcccacct gatcggcctg cggggcgtct tctggggccg cctggtgacc
     5281 gacctctcgg tcggcgccat cggcttcgcc tgggcctccc acgccctccg aaaggccaac
     5341 agcagcaaca taaaggaaga cggctggacc ccgatgtcct tcggggagta aacaccggcc
     5401 tcccgcaggt gtaatcggcc ttttcccttt tcacccgggg tggggtatac taatttttca
     5461 gagagccggg cgcaagaccg gcgaagaaaa ataaagacga tggacaaaac tacccgacac
     5521 cctaccgaat ccatcctgga gagcatctcc gacggcgtct tcaccgtgga cagcgagtgg
     5581 cgtatcacct ccttcaaccg ggcggccgaa aagatcaccg gcgtcccgcg ccgcgaagcc
     5641 gtcggacgac gctgttctga ggtcttccgc tgcagcatgt gcgaagccag ctgcgcgctc
     5701 cagaagaccc tgaaaaccgg caagccgatc atcggaaaat ccggatacat cataaatgcc
     5761 gaaggcaacc gcatcccgat cagcgtttcg accgcggttc tcaaggacaa ccggggccga
     5821 acgatcggcg gcgctgaaac cttccgcgac ctgagcgagg tcgaggccct gcgccgcgaa
     5881 ctggagggaa aggcccaggt cggcaacctg accagccgca gccctctcat gcagaggata
     5941 ttcgaggtac tgccggctat cgccgccagt cccagcacgg tcctgatcct gggcgagacc
     6001 ggtaccggca aggaactggt ggcgcgaacc gtccacgacc tgagcccgcg ccgcgactcc
     6061 cccttcttgg cggtcaactg tggagccctt cccgacacgc tcctggaatc ggaactcttc
     6121 ggctacaagg ccggcgcctt caccgacgcc cggaaggaca aacccggccg ctttgccctg
     6181 gccgggggag ggaccctctt cctggacgaa atcggcgaaa tcagcccggc cctccaggtg
     6241 cgcctgctgc gggtactcca ggaacggacc tacgaaccgt tgggagccac cgggtccgaa
     6301 acgaccgatg cccggatcat cgtcgccacc aacaaaaacc tggacgaagc gacccgacgc
     6361 ggcaccttcc gagaagatct ctactaccgc gtcaacgtgg tccggattga attgccgcca
     6421 ctgcggcggc gcaaggagga tatccccctg ctggccgagc agttcatcgc ccgtttcaac
     6481 cgcctccagc gcaaatcggt caccggactc tcctccgagg ccctctcgct cctgatgggc
     6541 cacgattggc cgggcaacat ccgcgaactc gaaaacatca tcgagcgcgc cttcatcctc
     6601 tgtcccgaag gacaggtcgg catcgagcac ctgccggccg aacttaccgg ccgggacgct
     6661 tcggttcact ccaaaaaccg tctccgcttg agccgggacg tcctggaggc ccaggccatc
     6721 cgggaggcgt tggagaagaa ccggttcaac cgcctggccg ccgcccgggc actgggcgtc
     6781 cacaagagca cccttttccg caagatcgac cggttgggca tcaaggtccc gaaggcggac
     6841 cggaaagact cccggggcat caagtcgcat tgatgctacc attttaaaag gccggggtag
     6901 caaaaaagcg actcacctca tccccgattt catcctttcc aagggctttg cccgcgacaa
     6961 aaatccgcgt tttcaatgcc ccccgtcttc cttcctttat cctggcaaaa aaattgcttc
     7021 ttgaaaagtg gccgcccgaa cgatcgggaa ggcaaaagat gaaaatcgcc ctgtccatct
     7081 ggaacgaaag aatcgcgccg gtcttcgatg tttcaagccg gatcagcctc atcgaggttg
     7141 acggagagcg ggtcacctgg aaatcggccg aggaactgcc cggagccgcc tccttcgcgg
     7201 cggccgcctg gctggccgag cagggggtcg aaacgctcat ctgcggggcg gtttcccggc
     7261 cggtgctggc caggttgact ggctacggca tcagggtcat acccttcgtg gccggcggcc
     7321 ggcgcgaagt ggtcgaggcc tggctgaacg gaaccctgga aagaaaacct ttcctgatgc
     7381 ccggctgccg ccggggcggc cccggtcccg gccgaagagg ctgtcgcggc cggggacatg
     7441 gcgggatggc ctccggctgc ttatggcagg aaacggcggc ggcgcggatc cgaacgaaag
     7501 agttcaaacc aagccaaaaa ggaggttgag atgccaagag gcgatggaac cggaccggcc
     7561 ggattgggac ctgggaccgg ccggaggggc ggcggcgccg gacgacccgg cggcggacgc
     7621 ggattgggaa gaaggggcgg cccgctggcg gccggcccgg gcggttattg cgtatgcgtc
     7681 aagtgcgggg aacgtctccc gcacgaacgc ggcgtgccct gcgtcgagca caagtgcccg
     7741 aaatgcggcg gcgccatgac cagggaagta taatcacctc aactgcaaag ggaggcaaaa
     7801 atgccgaggg gagatggaac cggaccgatg ggacagggtc cgatgagcgg acgagcagcc
     7861 ggatactgcg ccggttatgc cgtccccgga tacgccaatc agattccggg ccgcggcctg
     7921 ggccgaggtg ccggccgtgg atacgccgcc tggggccggg gttgcttcgg cggcggccgg
     7981 ggctggcgca actggtacca tgccaccggt ctgcccggct ggatgcggta taattacgcc
     8041 gcgccggcgg cggaccccgg cctcgagaag caggcgcttc agaaccaggt cgagatcctg
     8101 gagtcccagc tggacctggt gaaaaagcga atctcggaac tggagaaggc gccggagaaa
     8161 gagtagggtg atcgagggcg gccgtggcgg acctgccgcg gccgccctca caccagcgct
     8221 caccgcggta atgatggaca aaaaaatccg tggaaacagg cgataaaaag gaggcgcatg
     8281 aaaattgtct tcacaacttc tggcgataac ctccaggcgg aactggatgc ccgtttcggc
     8341 cgggctccga aattcctggt ctatgacctg gagaaggaac attacctggt ggttgacaac
     8401 cagcagaacc tccaggcggc ccagggcgcc ggcatccagt cggccgaaac ggtggccaag
     8461 ctcggcgccg gcgcggtggt caccggccat tgcggcccaa aggccttccg ggtcttgaag
     8521 gcggccggca tcaagattta caataccgac gcggctacgg tggccgaggc cctcaatcgc
     8581 taccgcgccg gagaactgac cccggccgaa tcggccgacg tggagggaca ttggagctga
     8641 atctggaaat aataacctac cggcccatcg gcatcatccg gagtccgcac cagaaacccg
     8701 aggagacgcc gatccagccg gcctacgccc ggggctgccc gggccgggcc gaagtctttc
     8761 cggaattcgc cgacggcctg cgcgatctgg agggcttttc ccacctctac ctgatctttc
     8821 acctccaccg ggccggtccg gcgaaactcc tggtcaagcc cttcctccag gacgtcgatc
     8881 ggggcgtctt cgccacccgc tctccgcggc ggcccaaccc gatcgggctg agcatcgtac
     8941 gcctggtcgg ccgtgaagga agcgtcctgc ttctgaagga cctggacatc ctggacggta
     9001 cgcccctgct ggatatcaag ccctacacgg ccaagttcga cg
//
LOCUS       DAOQYN010000061         8907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8326_length_8907_cov_4.145165, whole genome shotgun sequence.
ACCESSION   DAOQYN010000061 DAOQYN010000000
VERSION     DAOQYN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8326_length_8907_cov_4.145165"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1960)
                     /gene="pabB"
                     /locus_tag="PKN80_03645"
     CDS             complement(119..1960)
                     /gene="pabB"
                     /locus_tag="PKN80_03645"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNQ35140.1"
                     /translation="MAAASPARKNFVLLEDLRPGRGRRRRLLFHDPVEIVRAEEPAAV
                     PAALEKLQEALKQGFYVAGHLDYEAGYGLDPFWEKRPKNRAAAGRPLLWFGVYRKPDT
                     CPNPADSRFPIPSRPFYLGPLKLDTGWKEYARRAARVKEYIARGDVYQVNYTCRYRFD
                     FRGDPLDLYRALHSRQSAGYAAFIRQGRLSILSLSPELFISLRGGLIVTRPMKGTAGR
                     GRSPAEDAARVRRLAASSKERAENLMIVDLLRNDLGRICRTGSVRVTGLYEIESYETL
                     HQMTSTIEGRLKRVDLPRIFAALFPCGSVTGAPKLRAMEIIAEIEKSPRGPYCGAIGW
                     LGPGGRGLFNVAIRTLVIEDGRGEMGVGGGLTWNSRPAAEYRECRLKGDFLESVQAKS
                     TARFRLLETLRWQGRTYLYRREHLARLKNSAAFFGFQCDLGKVNRALDAFAGRLEGGE
                     YRVRLLLNRAGRIKIEAARLKETDAAPGLSRAAFYPAPVNSQNPFLYHKTTRRRLYNR
                     AHRLATDRGLFDFIFKNERGELTEGAITNLFIEKNGRFFTPPAACGLLNGIWRREFLA
                     AHPTASERILHPEDLAAADAVYLGNSVRGAVRIELVPAAAAGKVVIK"
     gene            complement(2041..2955)
                     /locus_tag="PKN80_03650"
     CDS             complement(2041..2955)
                     /locus_tag="PKN80_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNQ35141.1"
                     /translation="MDLKIRVAALNFRPWKWHKDWNAGRLEAFFRLAAGRGARLAVAP
                     EGILEGYLANEAIRWPELRRPMLETAEPPDGPYIRRFQDLARELETCLVFGYAELRNR
                     HDVYNAAIFIDHRGRVCGRHYKTEFQEGIDPAWNFNRPGERLRAFDTPLGRCGIMICY
                     ERRNPLVARVLALDGARFFCIPTFGADDPAQDRIVLARARENGLPAIQANVNRNLIIS
                     EGEPAALESGADRITLADVLIPERPSKEAARELEKEFFRRRVSRMNENRERRRLSKAA
                     GTAAAYVRPRRPANRKIRIGGPAAPKKS"
     gene            complement(2959..4230)
                     /locus_tag="PKN80_03655"
     CDS             complement(2959..4230)
                     /locus_tag="PKN80_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35142.1"
                     /translation="MERPAGFSPHLTYHERDEAAFREKKPLEVVFHAIGGFMDDEEAR
                     KRAAGENLPVRSYLHWHNDGARMGIPNYFIMLEYDNRYQLLLDDLFRPDTGLFSRTWW
                     FGYNEFVHEPEKMAQGRVYPYTHRRLEKTIAWASENYPVDRNRIYGHGASMGATGLMI
                     FALTHPGLFSAVDARVPAFNLGRLIKEKRERTYPDIWGPPEAAIKYQDGTDLDEYLDL
                     GRMLRRNPAAGYPFIRTYSGRTDLWMHWHFNPDFYRAMDEAGLTRLVTWSHLGHSGQV
                     EKILGESGVVDFQSFYNDRPLVGFRNSNFAADPGDGKIGSGAPTGVIGRGFKAEVSEE
                     SPERLVLKFETDGEVTRIDLVPRRLQKFLIKPGQRFSGSIRLNNRDFWTGRLEADQNG
                     LLFIPIDLSGLPENPGIPFKRRLVITITPAS"
     gene            complement(4432..4656)
                     /locus_tag="PKN80_03660"
     CDS             complement(4432..4656)
                     /locus_tag="PKN80_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35143.1"
                     /translation="MILKSARLPIPILCLFLLTTMLVPAARAAALSDFRYAVHEDRVY
                     IAWRESGDFDSVRVAVKKSGGTEVESIIPY"
     gene            complement(4805..7375)
                     /gene="mgtA"
                     /locus_tag="PKN80_03665"
     CDS             complement(4805..7375)
                     /gene="mgtA"
                     /locus_tag="PKN80_03665"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNQ35144.1"
                     /translation="MKLNSNNNHKKQTWDFDYIGCPPELLFEKLSTAPKGLTEAQANE
                     RIEEYGYNEPARPKKRTIIFEIFSKFLNPLVIVLLIIAGFSLFFGEKISAILVVIMAL
                     ISIFLSFIQEHRASKEAEKLSEMVRTTATVYRNGKAREIRIREIVPGDVVDLYAGDMI
                     PADLRIIVAKDLFINQASLTGESFPVEKNPEIPSTSKRSPADLTNIAFMGSSVVSGTG
                     LGMAVKTGLSTQFGEISKKLATISIASSFDKGIQAFTWLMIRLMIILVLIIFGINAFN
                     KGNIIQALLFALSVAVGLTPEMLPMLVAINLSKGAIAMSKKEVIVKHLNAIQNFGAMD
                     VLCTDKTGTLTMDKIVIEHHCDVVRREDDDVLRLAYINSYYQTGLKNLLDRAILRHGK
                     LVVKQYQKIDEVPFDFSRKLMSVVVGEINQHRLIVKGAPEEIYKRCTRFELDGEVLEL
                     DDGNLILPELKKEYDNLSAEGFRVLAVAYKDFDQPKDKYTKDDEQSLILKGYIAFLDP
                     PKASARRTIERLKNLGIDFKVLTGDNELVTNKICAEVGLKIKGFVTGDQVEKASDADL
                     RELVKTTTIFARLSPLQKERVIHALHDNRHIVGYLGDGINDAPALKTADVGISVNNAV
                     DIAKESADIILLKKSLLVLEDGVLEGRRTFGNILKYIKMGSSSNFGNMLSMTGASLFL
                     PFLPMLPIQILLNNLLYDISQIAIPSDEVDKEYLAKSRPWNVDYIKKFMLFIGPVSSL
                     FDFATFGVLWFIFHARQPLFNTGWFLESLCTQTLVIHIIRTDKIPFIESKPSQFLMFT
                     SIYIATIGLVLPFIPLGKHFGFVQPPPGYFIALIIIVAAYLITVQHMKNWFLKKYGRD
                     "
     gene            complement(8008..8448)
                     /locus_tag="PKN80_03670"
     CDS             complement(8008..8448)
                     /locus_tag="PKN80_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNQ35145.1"
                     /translation="MGTKRVNKAVDCFKSGFSCSQAVFSAYAEESGLDKNVALKISGT
                     FGGGMAGMAETCGAVTGAFMVIGLKYGKIMPEDEEAKKKAYSLVKKFVEKFKAKNSSI
                     VCKELLGCDISTPEGMNAFKDRDFINSMCPKFVENAVEILEEIL"
     gene            complement(8526..8840)
                     /locus_tag="PKN80_03675"
     CDS             complement(8526..8840)
                     /locus_tag="PKN80_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35146.1"
                     /translation="MARSRPSAKKSGETKTSPPRKLARRKQVRQETWRDKADPQNPTL
                     TQGFDGSREVERGFDKLSLDKANFDRLGTHFTAMKRASLCFMAIVVSESYSDKPANSA
                     CA"
BASE COUNT         1813 a         2496 c         2571 g         2027 t
ORIGIN      
        1 tgcccgattt tctctctcag actcattgac ttcagccgct tttcgaaatt gcgaaccatc
       61 ggtgcctcca gggatgtgcg tggaagatcc gtattaaggg cgagctaata ccagatatct
      121 attttatcac aaccttaccg gccgcagccg ccgggacgag ttcaatccgt actgcgcccc
      181 ggaccgagtt ccccaggtaa accgcgtcgg ccgccgcaag gtcttcggga tgcagaatcc
      241 gttccgaggc cgtcgggtgg gcggccagga attcccggcg ccagatgccg ttcagcaggc
      301 cgcaggcggc cggcggagtg aagaaacggc cgtttttttc aatgaagaga ttggtaatgg
      361 ctccctcggt taattcgccc cgttcgtttt tgaagatgaa atcgaataat ccccggtccg
      421 tggccaggcg gtgtgcccgg ttgtaaagac gccggcgggt ggtcttgtga tataggaagg
      481 ggttttggga gtttaccggc gccggataaa aggccgccct ggaaaggccg ggggccgcgt
      541 ccgtttcctt caatcgggcg gcctctattt tgatccggcc cgcccggtta agcagcagac
      601 gcacccggta ttcgccgcct tccagccggc cggcgaaagc gtcgagagcc cggttgacct
      661 ttcccaggtc gcattggaat ccgaagaagg ccgctgaatt cttcagccgg gccaggtgtt
      721 ccctccggta gagataggtt cggccctgcc agcgcagggt ttccagcagg cggaagcggg
      781 cggttgattt cgcctgcaca ctttccagga aatcgccctt caaccggcat tcccggtatt
      841 cggcggccgg ccgggagttc caggtaaggc cgccgcccac ccccatttcg ccgcggccgt
      901 cttcgatgac cagggttcgg atggcgacgt tgaagagtcc gcggccgccg gggccgagcc
      961 agccgatcgc cccgcagtaa gggccacggg gcgatttttc gatctcggcg atgatctcca
     1021 tggcccgcag tttcggagct ccggtgaccg agccgcaggg gaagagggcg gcgaagatcc
     1081 gtggcaggtc aactcttttc agccgcccct cgatcgtcga ggtcatctgg tggagggttt
     1141 cgtaactttc gatctcatag agcccggtca cccggacact cccggtccgg cagatccggc
     1201 ccaggtcatt tctgagcagg tccacaatca tcaggttttc ggcccgttcc tttgaactgg
     1261 cggccagtct tcgcacccgg gcggcatctt cggccgggct cctgccccgg ccggcggtcc
     1321 ccttcatcgg gcgcgtgacg atgagtccgc cccgcaacga gatgaagagt tccggggaaa
     1381 ggctgaggat ggagaggcgg ccttgcctga tgaaggcggc gtaaccggcg ctctgcctgg
     1441 aatgcagggc ccgataaagg tcaaggggat cgccccggaa atcaaaacgg taacggcagg
     1501 tgtagtttac ctggtaaaca tcgccccgcg cgatgtattc ctttacccgg gccgcccggc
     1561 gggcatattc cttccagccg gtatccagtt tcagggggcc gagatagaag gggcggctgg
     1621 ggatcgggaa tcggctgtcg gccggattcg gacaggtatc aggcttgcgg tagactccaa
     1681 accagagcag cggccggccg gcggccgccc gatttttcgg ccgtttttcc cagaaagggt
     1741 ccaggccgta tccggcttcg taatcaagat gaccggccac gtaaaacccc tgcttcaaag
     1801 cttcctgcag tttttccaga gccgccggga ccgcggccgg ttcttcggcc cggacgattt
     1861 caaccgggtc gtggaagagg aggcggcgcc ggcggcctcg tcccggcctc aagtcttcca
     1921 gcagaacgaa gttttttcga gcgggggaag cggcggccat ttttttatcc gggccttgcc
     1981 tgggggcggc ggacaaccgc tgctcacccg gcgttttcag gcaggcggtt cgggagtcct
     2041 tcaggatttt tttggagcgg ccggaccgcc gatccgtatc tttcgattgg ccggccggcg
     2101 cggccggaca taagcggcgg ccgttccagc ggcttttgaa aggcgtctcc gttcccggtt
     2161 ctcattcatg cgggagaccc ggcggcggaa gaactctttt tccagctccc gggccgcctc
     2221 tttcgagggg cgttccggga tgagcacgtc cgccagggtg atccggtcgg ccccggattc
     2281 cagggcggcc ggctcgcctt ccgagatgat caggttgcgg tttacgttgg cctggatcgc
     2341 cggcagtccg ttctctcggg cccgcgccag cacgatccga tcctgggccg gatcgtcggc
     2401 tccgaaggtg gggatgcaga agaagcgggc gccgtccagg gcgagaacgc gggcgaccag
     2461 gggattgcgc cgttcgtaac agatcatgat cccgcagcgt cccagcgggg tgtcgaaggc
     2521 gcgcagcctt tccccgggac ggttgaagtt ccaggccggg tcgatccctt cctggaactc
     2581 ggtcttgtaa tggcggccgc agacccggcc acggtggtcg atgaagatgg cggcgttgta
     2641 aacatcatgt cggttccgga gttccgcgta gccgaagacc aggcaggttt ccagttcccg
     2701 ggccaggtcc tggaagcgcc tgatataagg tccatcgggc ggttcagccg tttccagcat
     2761 tggccggcgc agttccggcc agcggatcgc ctcgttggcc aggtatccct ccaggatgcc
     2821 ctccggggcc acggccagcc gggcgccccg gccggccgcc agccggaaga aggcttccag
     2881 ccggccggcg ttccaatcct tgtgccactt ccaggggcgg aagttcaggg ccgccacccg
     2941 tattttcagg tccattcttc aggaggccgg ggtgatcgtg atcaccagtc ggcgtttgaa
     3001 ggggatcccg ggattctccg ggagtcccga caggtcaatc gggatgaaga gcaggccgtt
     3061 ttggtcggcc tccagccggc ccgtccagaa gtcccggttg ttcaggcgga tcgaaccgga
     3121 aaagcgctgt cccggcttga tcaggaattt ttggagacgc cttgggacca ggtcgatccg
     3181 ggtcacttcg ccgtcggttt cgaacttcaa gaccagccgt tccgggctct cctcgctcac
     3241 ctcggccttg aagccccggc cgattacgcc tgtcggcgcc ccggagccga tcttgccgtc
     3301 gccgggatcg gcggcgaaat tggaattgcg gaagccgacc aggggccggt cgttgtagaa
     3361 agactggaaa tcgacgacgc cggactcccc caggatcttt tccacctggc cgctgtggcc
     3421 caggtggctc caggtcacca gccgggtcag cccggcctcg tccatcgccc ggtagaaatc
     3481 cgggttgaaa tgccagtgca tccagagatc ggtccggccg ctgtaggtgc ggatgaaggg
     3541 ataaccggcg gccggattgc ggcgcagcat ccggcccagg tccaggtact catccaggtc
     3601 ggtgccgtcc tggtacttga tggcggcttc cggcggcccc cagatgtccg ggtaggtccg
     3661 ctcccgtttc tcctttatca accggccgag gttgaaagcc ggcacccggg cgtcaaccgc
     3721 gctgaaaagc cccggatggg tgagggcgaa gatcatcagg ccggtggcgc ccatcgaggc
     3781 gccgtggccg taaatccgat ttcggtcaac cgggtagttt tccgaggccc aggcgatggt
     3841 cttctccagt cgccggtggg tgtaaggata aacccggcct tgggccatct tctccggttc
     3901 gtggacgaac tcgttgtagc cgaaccacca ggtgcggctg aaaagaccgg tatcggggcg
     3961 gaaaaggtca tccagcagga gctggtaacg gttgtcgtac tcgagcataa tgaaataatt
     4021 gggaataccc atccgggcgc cgtcattgtg ccagtgaagg taagatcgca ccggcagatt
     4081 ttcgcccgcc gcccgtttcc gggcttcctc gtcgtccatg aagccgccga tggcgtggaa
     4141 gaccacctcc agcggcttct tttcccggaa ggcggcttcg tcccgttcgt gataggtaag
     4201 atggggtgaa aagccggccg gtctttccac ccggacgttt ttaagcgccg cggccgcctc
     4261 gaccggtttc ccgccccggc agggcgtgaa ccggtattcg tagaggccgg ccgcctccgg
     4321 gcaatggacg aagaggccgc tttccagcgt cagcggcagc tccgccggca gtttccaggt
     4381 cttgaccggg agatccttgt gcagccggct gtaatagtca accgcgctgt ttcaataggg
     4441 gataatgctt tccacttccg ttccgccgga ttttttcacg gccacccgga ccgagtcgaa
     4501 atcgccgctc tcccgccagg cgatgtagac ccggtcttcg tggacggcgt aacggaagtc
     4561 gctcagagcg gcggcgcggg cggccggaac cagcatggtc gtcagcagga acaagcagag
     4621 aatcgggatg ggaagtctgg ctgatttcag gatcatcggg cctccatggt ttgcctgctt
     4681 cctgaggtta tctgcgacgt cttttgcaaa cggtgcgttc ccgcccggtg agatcgaagc
     4741 gtccgtcaaa ccgcccgttt ttaacattgg aacggcggtt gaaacgcttg acgccggatt
     4801 caatctaatc acgaccgtat tttttaagga accagttttt catgtgttgc accgtgatca
     4861 aataggcggc cactattatt atcagcgcga taaaatagcc cggcggcggt tgaacaaatc
     4921 caaaatgttt tccaagcggg ataaacggca gtacgagccc tattgtagcg atgtaaatag
     4981 acgtaaacat caaaaactgg ctgggcttgc tttcgatgaa cgggatctta tcggtgcgga
     5041 taatatggat aaccaatgtc tgcgtgcaga gtgattccag gaaccacccc gtattaaata
     5101 acggctgtcg ggcgtgaaag ataaaccaca acaccccgaa tgtcgcgaaa tcgaagaggg
     5161 agctcacggg gccgataaac agcatgaact tcttgatgta atcgacattc cacggtcttg
     5221 atttcgccag gtattccttg tcaacctcgt ccgagggaat ggcgatctgc gaaatgtcat
     5281 aaagaaggtt attcaagagt atctggatgg gcagcatggg cagaaaaggc aaaaacaggc
     5341 tcgcgccggt catgcttagc atattgccga aattggagct ggagcccatt ttgatgtact
     5401 tcagaatatt gccgaaagtt cttcgtcctt ccagaacacc gtcctcaagg accaggaggc
     5461 ttttctttaa aagaatgata tccgccgatt ccttggcgat gtccacggca ttgttgactg
     5521 aaatcccgac atccgccgtt ttaagggcgg gagcgtcgtt gatgccgtca cccagatatc
     5581 ccacgatatg cctgttatca tgcaaggcgt gaataacccg ttctttctga agcggtgaaa
     5641 gacgggcgaa gatagttgtc gtcttaacca gctcccgcag gtcagcgtcg cttgcttttt
     5701 caacctggtc gcctgtcacg aagcctttaa tcttcagccc gacctcggcg catattttat
     5761 tggtgaccaa ttcattgtcg ccggttaaaa ccttgaaatc aatgccgagg tttttaagtc
     5821 tttcgatcgt tcttctggcc gaggccttgg gcggatcgag aaaagcgatg taccccttga
     5881 ggatcaggct ctgctcatcg tctttcgtgt acttatcttt tggttggtca aaatccttgt
     5941 aagcgaccgc cagcacgcgg aaaccttcgg cgctcaaatt gtcatactct ttcttcaact
     6001 cggggaggat caggtttccg tcatccagct ccagcacctc gccgtcaagt tcaaaccgcg
     6061 tgcatctctt gtatatttcc tcgggcgcgc ccttgacgat gagccggtgc tgatttattt
     6121 cccccactac cacggacatg agcttgcgcg aaaaatcaaa cggcacctcg tcgatcttct
     6181 ggtattgctt gacgaccagt ttcccatgcc ttagaatggc gcggtccagg agatttttca
     6241 agccggtctg ataatagctg ttgatgtaag caagcctgag cacgtcatca tcttcccggc
     6301 gcacgacatc acaatgatgc tcgataacga ttttatccat tgtcagcgtg ccggttttgt
     6361 ccgtgcaaag cacgtccatc gccccgaaat tttgaatggc gttgagatgc ttgacaatga
     6421 cttccttttt cgacatggcg atagcgccct tggaaaggtt gatcgccacc agcatgggca
     6481 acatctccgg cgtaaggccc acggcaacgg aaagggcgaa gaggagggct tgtatgatat
     6541 tgcccttgtt gaaggcatta atgccaaaaa tgataaggac caaaataatc atgaggcgta
     6601 tcatgagcca ggtaaacgcc tgaatgccct tatcgaaact gctggcgata ctgatggtgg
     6661 ccagcttctt tgatatttcg ccgaattggg tggacaggcc ggttttgact gccattccca
     6721 ggccggttcc acttaccacg cttgaaccca tgaaggcaat attggtcaaa tcagcgggcg
     6781 aacgctttga cgtggaaggg atttccgggt ttttttcaac tggaaaggac tcgccggtca
     6841 gggaggcctg gttgataaac aggtccttgg ccacaataat tctcaggtcc gccgggatca
     6901 tgtccccggc atacagatcg acaacgtcac ccggcacgat ttccctgatg cgtatttccc
     6961 gcgccttgcc gtttcggtag accgtggcgg tcgtgcgcac catttcgctc agtttttcgg
     7021 cttccttgct ggcgcggtgc tcctggatga aggaaaggaa gatgctgata agcgccatga
     7081 taaccactaa aatggcgctg attttctcac cgaagaagag ggaaaaaccg gcgatgatca
     7141 aaagaacgat aacaagcggg ttgagaaact tggagaaaat ctcgaaaatg attgtccgct
     7201 ttttggggcg cgccggttcg ttgtatccgt attcttcgat tctttcgttc gcctgtgctt
     7261 cggtcaagcc cttgggggct gtcgacaact tttcgaaaag cagttccgga gggcatccga
     7321 tataatcgaa gtcccaggtt tgctttttat ggttattatt cgaattaagt ttcattgccc
     7381 tggattgaat tgagatggca gcctgagaaa cttccgacaa gtcccgtgtt ttcttttctt
     7441 tacagcctta attctttaaa tcttcagctt ctggcgtcgg ctgatttcaa cccggcggcc
     7501 cctcgcaaat tgcgctggag ccgggttgcc cgaagaattg acggccgaac ttaattataa
     7561 tccattcacc aggattccgc catccgccgg acgtggcaag accggccttg attcaggcgt
     7621 gcgttccaga acgaacgccg ccggcgaaat tgcccgcggg atggctcctg gggaaatcgt
     7681 tccaaagcgg taagggtaag gaacaggatt catcttgttg gcggccgcgg gggaggggga
     7741 gtgcggttcc ggccgagggg aaaattaaaa aactatcacg atcggtcgcg tcgcctgacc
     7801 ccggtcctct aagatctccg ttaagagcgg ggttttgcgg cttgtcgttg atggcaggcg
     7861 aaccctaaaa ctacctgaaa tacagttact tatcaaaatc taccgcacaa gaagcacggg
     7921 ctggtagata tctttaggcg aaccggacaa tacttgttta ccatctaaaa atgacgtttt
     7981 atcgagacgc cacgaaaaaa ccgatgacta aagaatctct tcaagaattt ctacggcgtt
     8041 ttcaacaaac ttgggacaca tactattgat aaaatctcta tctttaaatg cattcatccc
     8101 ctccggggtg ctgatatcgc agccgagcag ttccttgcag acaatggaag aatttttagc
     8161 cttaaatttt tccacgaact ttttaactaa agaataggct tttttctttg cttcttcatc
     8221 ttctggcatt atctttccat atttgagacc gataaccata aaagcaccgg ttacagcacc
     8281 acacgtctca gccattcctg ccatgccgcc gccaaatgtt ccgctaattt ttagcgccac
     8341 atttttatca aggcctgatt cttccgcata agccgaaaag acggcttgag aacaactaaa
     8401 accgctttta aaacaatcga cagctttatt aactcttttt gttcccattc cggcctttca
     8461 aaacagtttg aatcccattc tactcaagcc aaaaatccag tcggctctcg tcggaagcct
     8521 gcttttcaag cgcaggcgga attcgctggt ttgtctgaat aagattcaga aacaacaatc
     8581 gccatgaagc agagcgaagc acgcttcatg gccgtgaagt gtgttccgag cctgtcgaag
     8641 ttggctttgt caaggctgag cttgtcgaag ccgcgctcta cttcacggct cccatcgaat
     8701 ccctgcgtaa gagtggggtt ttgcggatcc gccttgtctc gccaggtttc ctggcggact
     8761 tgttttcgtc tcgccagctt tcttggcgga cttgttttcg tctcgccaga tttcttggcg
     8821 gacgggcgag atctcgccat ttttcacttt gcgaaaaatg gcggagaggg agggattcgg
     8881 cgccctcgcc tcccactgcg gctcggg
//
LOCUS       DAOQYN010000062         8787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8462_length_8787_cov_4.367499, whole genome shotgun sequence.
ACCESSION   DAOQYN010000062 DAOQYN010000000
VERSION     DAOQYN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8462_length_8787_cov_4.367499"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKN80_03680"
     CDS             <1..366
                     /locus_tag="PKN80_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35147.1"
                     /translation="GIEHIHGAPVYRLRGNLLGREQHGFIEAVGFYLYSQLFREAVAE
                     LTGKAAGPAEPGGPVSGIIPADYIPSEMVRLSIYRRLFGLSEDADRLARELADRFGPV
                     PEETRKLLRSLYHNRPPLV"
     gene            385..456
                     /locus_tag="PKN80_03685"
     tRNA            385..456
                     /locus_tag="PKN80_03685"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:417..419,aa:Ala,seq:cgc)
     gene            1143..2372
                     /locus_tag="PKN80_03690"
     CDS             1143..2372
                     /locus_tag="PKN80_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35148.1"
                     /translation="MKTRQRIAYGLAAFLISLTLGVAVPARLRAAVEEIGLRGNYQEN
                     LKKTLENPALVDLRHPGLAYYWGEQFLVDFLGGAARKGKPASGAFPDQRPVRFHFLPK
                     EMSEQLAVKNIGGLTLLSIRYRPESGRVEFPLLITAETPLLLSFPASAGEYRELLTLY
                     KEQRLRLRLTGRIKEVRGVLVEKVPRAELEFITPGVEVLDLLTPKFARSNGVSAVPMP
                     ADYQVVEPEAVAGPKSPVRMRFAPDAFDLPYSEGMQPFRRGWILKDAFPRHRYETADI
                     PYGVIAVCEHPGLPASMTLRFEKPLPPGTYKISIETPYYRNRDFDNILRFRLGEEEVE
                     TAYRFGGRLPWALVPALRITRPARELTVTAVQAGGGGLSEAPPYYRYWIAIAQLFITN
                     DLNELDGKTQTPNPEAK"
     gene            2372..5455
                     /locus_tag="PKN80_03695"
     CDS             2372..5455
                     /locus_tag="PKN80_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35149.1"
                     /translation="MKTIQIGPLVLASLLILAGGAAAGPESGNLLRNGSFEVGWCHET
                     GRKITGFDSAFPDPGWITDEAAFHGRYALKLKPMPRRIADLSFNSSQFFTLPVTLAAN
                     KKYVLGLWARSVKGPAKISITPMTPGNRGTSLGPAASASLTDTDWTRLRVTVTGASKP
                     AYLLITGSDVLIDAVEFLPEDLALNDTAARETRYIPSSAVEAGIDDPAVGRIHYQEEP
                     PVTFEVRLYNYAGQAKSGTLVYELINQEKVITLQGELPPVQLAANGTAAVRLPLPALP
                     NGMYALKYWVKGSEAGWGETTFCVMPRPDPSRKAAVGLLSTISPEVVALMKRAGFGYY
                     GNLTDLYLRLENIYDVEKMSLPDRTELVKMMTDAGITVTMCINPWWWRKPAEPPWHPP
                     LPARHPSQGPDRETYFDAVDQFARMYAPYVKIWWVQDELEGHAHPHEFLPILVKTEEI
                     VRKHRPDAEFLISAECAWLDGFERVGGMPYVDWFGGSFMNYSGDRGRKLGWLIDKYQK
                     RMWVIAGSFSRDTTFSRLRGGAIHATYSAHHNFINSFFLHRAQVTMDYRTLSTNYSWM
                     EIHDPYNLFEPDTTLSTALALYAIAGQVLAGSQPDPDPLETPVNHGGYLWAYRDGRSG
                     RPAVCFFPGEARTVTIALDPKRLRLFDQLGNPLELEAAPEGKSRFRCEPNKYYFLHGI
                     EVEPAAFRETVRLAETDLPRLPRTESFAYYVADPKQTVRIEVLHVNRGQEPWTGHFRA
                     PAFKDGPVPSERSLREQIDTTFPYREDRPMNAQQLPPQQGRGFVWLNTARPLSKPPAL
                     DGDFGEWGTVASSALYYSMQVLGSVDMETIHSWKDSYQITCDYGMDYRVEWWAGYDAE
                     NLYLCGRVFDDQLVAADHPAGGKEPDCLELRFDREITGDLVQPDPGDDFSVWLVPATA
                     GGRELRLEISAADGSNRRTLEGSRGTIRIWDDPAVLKGWEPWPHPGPAVGYLIEAALP
                     WKSLGIDPKPGAIIGFDLFGHETDIEPDGKPAASVLRWAGNANPGGQLRLGTGRP"
     gene            complement(5526..7415)
                     /locus_tag="PKN80_03700"
     CDS             complement(5526..7415)
                     /locus_tag="PKN80_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35150.1"
                     /translation="MRHPEKEAALQSVRRVAEFLKSETAPVEPVVYRGFFSGPRYLFY
                     DRAKRRYHTWFDRRRRGWCVRVTPPAGDSFTLPQTGLHFLVTTRSFFVYANFPLAVEY
                     LENFFRRRDYPVDSLSWKETVSLPWWQAVQGTLVSGGAAGSFSINRSRGRFTARFRDE
                     NGRPCFCSPRHQYLFIFKPRLRIRDGRVRIEQLLKSYAETEIKTTSPNPLALFKQAVY
                     GFKSANLRAFYSEEGLIPEVRHVIIFVVYWHFALALINASVFVRVAERLKGQTWYTLA
                     AGALVFFRMPAEIINMRRALRLTRNLPGSRLQKIMDSEEFRSIIENRKIRSWRSFERV
                     YTDLFRATEYLAEALERYRLAKPKIYRLVSAGRQAEVPGVLESELGIGKEGLAMLAPL
                     AGRLEALERPEIVRIFTGHLERLRSAGLLRESEWPGLVDFVNRTFPEQLEWDTVVGAY
                     KSLNDRIKRAERNPSRPELSVMIENSKRASIPFRRAELLLMVSFYMIGYGATYLPYMP
                     TLVFPLYYLVYGVLTQLIYSGLERMGTPVSLQYLYKELENDPAVPSASDVWNEYASQQ
                     LRLFAVFSSLGLIIGIGGRLLADRTHNISWFAVEALGMGTYLLALWEWFRYWNGLVRR
                     CKVAH"
     gene            complement(7402..8682)
                     /locus_tag="PKN80_03705"
     CDS             complement(7402..8682)
                     /locus_tag="PKN80_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNQ35151.1"
                     /translation="MIFAIIVLVILLLFSAYFSAAETALTSLGKLKVKALIEKEGRRA
                     EVLNLWLKEPSRYLTTILVANNIVNIVAASLSTYLAIENVPEQYDTLATGIATFCITL
                     LILVMGEIVPKTFARQNSDRLALFFFPSMLSITYLIHPVVQFFVSFSDKIIRLLGGHL
                     APQATHISTEELQTLIDIGEEEGALAAEEREMLTSVIEFRETEVSEVMTPRVTISALP
                     EDASIGDALALMVKDKHSRLPIYRDSMDEIVGVVDSRQVLGYISEGERNLLVRQVMRP
                     PFFVPEMMRVSELLKEFKRRKLHLAIVIDEYGGTAGLVTLEDLLEEIVGEIQDEYEVE
                     SSEYQTLAENTYLANAKMEIEKANQLLKLDIEDDDTAETLAGFVLNRLGHIPQKGEQV
                     RYHNFLFTVEQADERTVNLLRIQKLTPRVPHEAS"
BASE COUNT         1667 a         2836 c         2795 g         1489 t
ORIGIN      
        1 ggcatcgagc acatccacgg tgccccggtc taccggctgc ggggcaacct gctgggccgc
       61 gagcagcacg gcttcatcga ggcggtcggg ttctacctgt acagccagct cttccgggag
      121 gccgtggccg aattgaccgg gaaagcggcc ggaccggccg aaccgggcgg gccggtctcc
      181 ggcatcatcc cggccgacta cattccctcg gagatggtgc gcctctcaat ctaccggcgt
      241 ctcttcggcc tctccgagga cgccgaccgg ctggcccggg aactggccga ccgtttcggg
      301 ccggtgccgg aggagacccg caagctcctg cgctcccttt accataaccg gccgcctctg
      361 gtataatatt gaagatcgtt gaatggggca gtagtttagt gggaaaacac tggaatcgca
      421 ctccagagac cgagggttca actccctcct gctccacttc ctgacaaagg gagttgatgg
      481 gaggccgttt acgaaaggcg accgatcagg gagcgtttcg tggccgggtg gccgaccgga
      541 gccccgccgg aattccggcg ggcggaggcg ccaactccct cctgctccac ttcctgacaa
      601 agggagttga taactgccaa tgcgacggat aagaagcatt cgcctggcag gggcggcgag
      661 atagtgaacc gcccggaccc cgcctgaaaa acccgccctt tcagagccgt cagccgggtg
      721 gccgaccgta gcctcgccac aattatggcg ggcggaggcg ccaacttctt tctgctccac
      781 tgagatttgc ctggataaat ctcagtagag aatcgaaagt cgaaagtcga aaattgataa
      841 ggtctggtta aggccggtga ttttcgatta ggtccattct cgattctcga ttttcaagtt
      901 ggcggaagcg ccaatttttc ccggttcccg gctgccctgc ctgatccatt cccgcttatc
      961 aatttcttct cctcgagcaa ccgcccggcc gctcccgtaa tttgacaggc ggccagtcct
     1021 gatgttattt tccaaaggta tcgccgttat cttctttcac ccacgaaccg gtgttcgtcg
     1081 gaggagcatg gttgctcaaa ttccgtccca ccctgaccgt taacagatca gcgaggtgaa
     1141 aagtgaagac gcgccaacgc attgcatacg ggctggccgc ttttctaatc agcctgaccc
     1201 tgggggtcgc cgtcccggcc cggctccggg ccgcggtcga ggagatcggc ctgcgcggca
     1261 attaccagga aaacctgaag aagacgctgg agaacccggc gctggtggac ctgcgccatc
     1321 cgggcctggc ctactactgg ggggaacaat tcctggttga tttcctgggc ggagcggccc
     1381 gtaaaggcaa gccggcttcc ggcgccttcc ccgaccagcg gccggtccgc tttcacttcc
     1441 tgcctaaaga gatgtcggaa cagctcgcgg tgaagaacat cggcggcctg acactcctct
     1501 ccatccgcta ccgcccggag agcggccggg tggagttccc gctgctgatc accgccgaaa
     1561 cacccctgct gctctccttc ccggccagcg ccggcgagta ccgggaactc ctgacgctat
     1621 acaaggagca gcggctgcgt ctccggttga ccggccggat caaggaagtc aggggagtcc
     1681 tggtggagaa ggtcccccgg gccgaactgg agttcatcac gcccggcgtc gaggtgctcg
     1741 acctgctgac gccgaagttc gcccggagca acggggtgag cgcggtcccg atgccggccg
     1801 attaccaggt ggtcgaaccg gaagcggtgg ccgggccgaa atcaccggtc cggatgcggt
     1861 tcgccccgga cgccttcgac ctcccctatt cggaaggcat gcaacccttc cggcggggct
     1921 ggatcctcaa ggacgccttt ccgcgccacc ggtacgaaac ggccgacatt ccttacgggg
     1981 tcatcgccgt ctgcgagcat cccggtctgc cggcctcgat gacgctgagg ttcgaaaaac
     2041 cgctgccgcc cggaacctac aagatctcga ttgaaacccc ctattaccgc aaccgggatt
     2101 tcgacaacat cctgcgcttc cgcctcggcg aggaagaggt cgagaccgcc taccgtttcg
     2161 gcggccgcct gccctgggcg ctggtgccgg ccctccggat taccaggccg gcccgggagc
     2221 tcaccgtgac cgccgtccag gccggcggcg gcggtctttc ggaggccccg ccctattacc
     2281 gttactggat cgccatcgcc cagctattca ttaccaatga cctgaacgag ctcgacggca
     2341 agacccagac cccgaacccg gaggcaaaat aatgaagacc attcagatcg gaccgctggt
     2401 cctggcctcc ctgctgatcc tggccggcgg cgcggccgcc gggccggaat ccggcaacct
     2461 gctgcgcaac ggcagtttcg aggtgggctg gtgccacgag accggccgga aaataaccgg
     2521 ttttgattcg gccttccccg acccgggctg gatcaccgac gaggccgcct tccacggccg
     2581 ctacgcgctg aaattgaaac cgatgccgcg ccggatcgcc gacctctcct tcaacagcag
     2641 ccagttcttc accctgccgg tcaccctggc cgccaacaag aagtacgtcc tgggcctctg
     2701 ggcccgctcg gtaaaagggc cggccaagat ctcgatcacg ccgatgactc cgggcaaccg
     2761 gggtaccagc ctgggtccgg ccgccagcgc cagcctgacc gacaccgact ggacccggct
     2821 ccgggtaacc gtcaccggcg ccagcaagcc ggcttacctg ctgatcaccg gcagtgacgt
     2881 cctgatcgac gcggttgagt ttctgccgga ggacctggcc ctgaacgata cggccgcccg
     2941 cgagacgcgc tacattccat cttcggcggt cgaggccggc atcgacgacc cggcggtcgg
     3001 gcgcattcat taccaggagg agccgccggt caccttcgag gtccgcctct acaactatgc
     3061 cggccaggcg aaatccggaa cactggtcta cgaattgatc aaccaggaga aggtcatcac
     3121 tctccagggt gaattgccgc cggtccagct ggccgccaac ggcacggccg cggtccggct
     3181 gcccctgccg gcgctgccca atggaatgta cgcgctcaag tactgggtga aaggcagcga
     3241 ggccggctgg ggagagacaa ccttctgcgt gatgccccgg cccgatccgt cccggaaggc
     3301 ggccgtcggt ctcctgagca ccatatcgcc ggaggtggtc gccctgatga aacgggccgg
     3361 cttcggctac tacgggaacc tgaccgacct ctacctgcgc ctggaaaaca tctatgacgt
     3421 cgaaaagatg agcctgcccg accggacgga actggtcaag atgatgaccg acgccggcat
     3481 cacggtcacc atgtgcatca atccctggtg gtggcgcaaa cccgccgagc cgccctggca
     3541 cccgccgctg ccggcccgcc acccgtccca gggtccggac cgggagacat acttcgacgc
     3601 ggtggaccag ttcgcccgga tgtacgcccc ctacgttaag atctggtggg tccaggacga
     3661 actggagggg cacgcccacc cgcacgaatt cctgccgatc ctggtcaaaa ccgaggagat
     3721 cgtccggaag caccggccgg acgcggagtt tctgatcagc gccgaatgcg cctggctgga
     3781 cggttttgaa agggtgggcg gcatgcctta cgtcgactgg ttcggcggct ccttcatgaa
     3841 ttattccgga gaccggggcc ggaaactggg ctggctgatc gacaagtacc agaagcggat
     3901 gtgggtgatc gccggcagtt tcagccggga caccaccttc agccggctgc gcggcggcgc
     3961 gatccacgcc acctactcgg cccaccacaa cttcatcaac agcttcttcc tgcaccgggc
     4021 ccaggtgacc atggattacc ggaccctgtc aaccaactac agctggatgg agatccacga
     4081 cccctacaac ctcttcgaac ccgacaccac cctgtccacg gccctggccc tctacgccat
     4141 cgccggacag gtgctggccg gcagccagcc ggatccggat ccgctcgaga cacccgtgaa
     4201 ccacggcggc tacctctggg cctaccggga cggccggagc ggccggccgg cggtctgctt
     4261 cttcccgggc gaggcgcgta ccgtgaccat cgcgctcgat ccgaagcgcc tgcgcctctt
     4321 cgaccagctg ggcaatcccc tggaactgga ggccgcgccg gagggtaaga gccggttccg
     4381 gtgcgaaccg aacaagtact acttcctgca cgggatcgag gtcgaaccgg ccgccttccg
     4441 ggagacggtc cggctggccg agaccgacct gcccaggctg ccacggaccg aatctttcgc
     4501 ctattacgtg gccgacccga agcagaccgt ccggatcgag gtgctgcacg tcaaccgggg
     4561 gcaggaaccc tggaccggcc atttccgggc gccggccttc aaggatggac cggttccctc
     4621 cgaaagatcg ctccgggagc agatcgatac gaccttcccc taccgggaag accggccgat
     4681 gaatgcccag cagctgccgc cccagcaggg aaggggtttc gtctggctga acaccgcccg
     4741 tcccctctcg aaaccgccgg ccctggacgg tgacttcggg gagtggggaa ccgtggccag
     4801 ttccgcgctt tactactcga tgcaggtcct cggcagcgtg gacatggaga cgatccactc
     4861 ctggaaggac agctaccaga tcacctgcga ttacgggatg gactaccggg tggaatggtg
     4921 ggccggctat gacgccgaaa atctctacct gtgcggacgc gtcttcgacg accagctggt
     4981 ggccgccgac cacccggccg ggggaaaaga acccgattgc ctggaactgc gcttcgaccg
     5041 ggagatcacc ggcgacctgg tccagccgga ccccggcgat gacttctcgg tctggctggt
     5101 cccggcaacg gccggcggcc gggaactccg cctggaaatc tcggcggcgg acgggtcaaa
     5161 ccggcggacg ctggaaggca gccgcggcac gatcaggatc tgggacgacc cggccgtgct
     5221 caagggctgg gagccctggc cgcatccggg accggcggtc ggctacctga tcgaagccgc
     5281 cctgccctgg aaatcgctgg gaattgatcc gaaaccgggc gcgatcatcg gcttcgacct
     5341 cttcggccac gaaaccgaca tcgaaccgga cgggaaaccg gcggccagcg ttctgcgctg
     5401 ggccggaaac gccaatcccg gcggccagct gaggctggga accggccggc cttaggaaag
     5461 accgatccgg gcagagtatc tttcaggata taaggcgggg aggaaacgac cgggaccggc
     5521 ccgtttcagt gggccacctt gcaacggcgc acgaggccgt tccagtaacg gaaccactcc
     5581 cagagggcca gcaggtaggt gcccatcccc agcgcctcga ccgcgaacca ggagatgttg
     5641 tgggtccggt ccgccagcag ccggccgccg atcccgatga tcaggcccag cgaggagaag
     5701 acggcgaaga ggcgcagctg ctggctggca tactcgttcc agacgtccga agccgatggg
     5761 acagccgggt cattttccag ctccttgtaa aggtactgga gcgaaaccgg ggtgcccatc
     5821 ctttccaggc ccgagtagat gagctgggtc agcacgccgt agaccaggta ataaagggga
     5881 aagaccaggg tcggcatgta ggggaggtag gtggcgccat agccgatcat gtagaaggag
     5941 accatcagga gcagttcggc gcgccggaag ggtatcgagg cgcgcttgga attctcgatc
     6001 atgacgctca gttccggccg gctgggattc cgttccgccc gcttgatacg gtcattcagc
     6061 gacttgtaag ccccgaccac cgtgtcccat tccagctgtt ccgggaaggt ccggttgacg
     6121 aagtccacca gtcccggcca ttcgctttcc cggagcaggc cggccgaccg cagccgctcc
     6181 aggtggccgg tgaagatgcg cacgatctcc ggccgttcca gggcctccag gcgcccggcc
     6241 agcggcgcca gcatggccag cccctccttc ccgatcccca gttcgctctc cagcacgccc
     6301 gggacctcgg cctgccggcc ggcgctcacc agccggtaaa tcttcggctt ggccagccgg
     6361 tagcgttcca gggcctcggc caggtactcg gtcgcccgga agagatcggt gtatacccgc
     6421 tcgaaactcc gccaggagcg tatcttgcgg ttctcgatga tggaacgaaa ctcctcggaa
     6481 tccatgatct tctggaggcg ggaaccgggc aggttccggg tcaggcgcag ggcccggcgc
     6541 atgttgatga tctcggccgg catccggaag aagaccaggg cgccggcggc cagggtgtac
     6601 caggtctgcc ccttgagccg ctcggccacc cggacaaaga cgctggcgtt gatcagggcc
     6661 agcgcgaagt gccagtaaac cacgaagatg atgacgtggc ggacttcggg tatcaatccc
     6721 tcctcggaat agaaggcccg caggttggcg ctcttgaaac cgtagacggc ctgcttgaag
     6781 agggccagcg ggttggggct ggtggtcttg atctcggtct cggcgtagga cttcaggagc
     6841 tgctcgatcc ggacccggcc gtcccggatg cgcaggcgcg gtttgaagat aaaaagatac
     6901 tggtggcggg gcgagcagaa gcagggccgg ccgttctcgt cacgaaaacg ggcggtgaac
     6961 cggccgcggg agcggttgat ggaaaaactc ccggccgccc cgccggagac cagggtcccc
     7021 tggacggcct gccaccaggg caggctgacg gtctccttcc aggacaggga gtcgaccggg
     7081 tagtcgcggc ggcggaaaaa gttttccagg tactcgaccg ccaggggaaa attggcataa
     7141 acgaaaaaac tgcgggtggt gaccaggaaa tggagtccgg tctggggcag ggtaaacgaa
     7201 tccccggcgg gcggagtcac ccggacgcac cagccccggc ggcgtcggtc aaaccaggtg
     7261 tggtaacggc gcttcgcccg gtcgtagaag aggtaacggg ggccgctgaa gaagccgcgg
     7321 taaacgaccg gctcgaccgg ggcggtctcc gacttcagga attcggctac ccggcggacg
     7381 gactggagcg cggcctcctt ctcaggatgc ctcatgcgga acacgggggg tcagcttctg
     7441 gatgcgcagc agattgacgg tccgctcgtc ggcctgctcc acggtgaaga gaaagttgtg
     7501 ataacggacc tgttccccct tctgggggat atggccaagc cggttgagaa caaagccggc
     7561 cagggtttca gccgtgtcgt catcctcgat gtccagcttc aacagctggt tggccttctc
     7621 gatctccatc ttggcattgg ccaggtaggt attctcagcc agggtctggt actcgctgct
     7681 ctccacctcg tactcgtcct ggatctcgcc gacgatctcc tccagcaggt cctccagcgt
     7741 caccaggccg gcggtgccgc cgtactcgtc gatgacgatg gccaggtgca gcttgcgccg
     7801 cttgaactcc ttgagcagct ccgagacccg catcatctcg ggcacgaaaa agggcggccg
     7861 catcacctgg cgcaccagca ggttgcgctc cccctccgaa atgtatccca gcacctggcg
     7921 cgaatccacc acgccgacga tctcgtccat ggagtcgcgg tagatcggca ggcggctgtg
     7981 cttatccttg accatcaggg ccagggcgtc gccgatcgag gcgtcctcgg gcagggccga
     8041 aatggtcacc cgcggggtca tcacctcgga gacctcggtc tcccggaact cgatcacgct
     8101 ggtgagcatc tcccgttcct cggcggccag cgccccctcc tcctcgccga tgtcgatcag
     8161 ggtctggagc tcctcggtgc tgatgtgggt cgcctggggc gccaggtggc cgcccagcag
     8221 ccggatgatc ttgtcggaga aggagacgaa gaattgaacc accggatgaa tcaggtaggt
     8281 gatcgaaagc atggacggga aaaagaagag ggccagccgg tcggaattct gacgggcgaa
     8341 agtcttggga acgatctcgc ccatgaccag tatcagcagg gtgatgcaga aggtggcgat
     8401 cccggtggcc agcgtgtcgt actgttcggg aacgttctcg atggccaggt aggtggataa
     8461 ggaggcggcg acgatgttga cgatgttgtt ggccaccagg atggtggtca ggtacctgga
     8521 gggctccttg agccagaggt tgagaacctc ggcccggcgg ccctcctttt caataagggc
     8581 cttgaccttc agttttccga gcgaggttaa ggcggtctcg gcggccgaaa agtaggcgga
     8641 gaaaagcagc aggatgacca gtacgatgat tgcgaatatc atataatcac gtaaattata
     8701 ctataaaacc ggcctttcgt aaagccgaac cgccggtcag ccgttcaatc ccagcaggcg
     8761 gcgggcattg ccggccaggg cggccgc
//
LOCUS       DAOQYN010000063         8727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8538_length_8727_cov_4.266490, whole genome shotgun sequence.
ACCESSION   DAOQYN010000063 DAOQYN010000000
VERSION     DAOQYN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8538_length_8727_cov_4.266490"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKN80_03710"
     CDS             <1..221
                     /locus_tag="PKN80_03710"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNQ35152.1"
                     /translation="RAGLLVSAYHDQILPLFKARYFHSGVNVTIGLPLIRTSPDHGTA
                     FNLAHRNLASSASMQAAIRLALKLACRA"
     gene            227..1078
                     /gene="rsmA"
                     /locus_tag="PKN80_03715"
     CDS             227..1078
                     /gene="rsmA"
                     /locus_tag="PKN80_03715"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNQ35153.1"
                     /translation="MAGLRPKKKFGQHFLTRPVLAARIAEFAGLKPGDTVVEIGAGTG
                     LLTRELARRAGRVLTFEIDRELLAGLNSELAQHPNVEVMPADFLKAGPERLKTLGEFK
                     VVANLPYGVATPIIFQLLKFLRPEAGTASLKTAVLTVQKEVARRLTAPAGGRDYGPLS
                     LAVQAGSEVEYLLTIKPGAFHPPPKVDSAVIRLRPRSHPAAEPEILEAALKLARTAFQ
                     QRRKTILNGLAAGLKRSKTEAAAQLADCGIPSTARPEELSLEDYLRLARTVKDRGVAG
                     KNRARSG"
     gene            complement(1075..3066)
                     /locus_tag="PKN80_03720"
     CDS             complement(1075..3066)
                     /locus_tag="PKN80_03720"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HNQ35154.1"
                     /translation="MKTGDGRWYQSRLFRLQSGSSRRLEYDLTSSSTDWQPHGHSETW
                     SDSALYSVERIGLKFFGTRVGPGLILLDDFRVAGRTLTFLEQDRKQTGLYERFELTFG
                     LDRSFENPFDTDLVRIDAHFLAPSGREWVVPAFFYQGYEFEGPGRNSQDRLIPVGVPV
                     WKARFSPVEIGRHRCFLEISLDKGAEKFRSPVREFEAVVSGRRGFLRVNQRDRRHFAF
                     DDGTSFYPIGHNIRSLNDNRYAGFRRAPLAASEGTLAMEDWLKSMAANGENFFETWMA
                     AWWLALEWKKDSNYEPNNFYEGIGRYNLRNAWKLDWILEHSEALGIYIQLLIVNHGSC
                     STFCDQEWNDNPYNRKNGGFLDSPDDFFRDPEARRLFQQRLRYIVGRWGYSPNIFAWE
                     LLNEMNLVGASGAFYQTPGFRDWYQVMIDYLKETDPWGHLVTGHYTILYDSDVFKLPE
                     VAYTLTNAYYGGRNGLAATLSNAARFLERFDKPHFVSEYGGSSGGGDEDLLKADLHNG
                     LWIGYHLPFAAPPLFWWHNFIEEQNLFFHYRALANYAAGEDRSLEKLVPARVALNGEG
                     VEKTASFCLKGSRRAFIWFYDQGQLSRLPAEDGLPFFKQARASLEGLESGDYRLEFWD
                     TYRGVIFEERRVKGQGRLEFDLPPVNRDFAVKIKPLSNP"
     gene            complement(3496..3966)
                     /gene="rpsG"
                     /locus_tag="PKN80_03725"
     CDS             complement(3496..3966)
                     /gene="rpsG"
                     /locus_tag="PKN80_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNQ35155.1"
                     /translation="MGRRRKRIKRELLPEPKYGSLEAAKFINMMMYSGKKSTCQASFY
                     KSMDILSEKTKENPLAVFKKALENVRPRVEVRSRRIGGATYQVPVEVPSSRSLALAIR
                     WLLAVSRKKKGKPMYEKLANELLDAYKGEGETIKRRDELHRMAEANRAFAHYRW"
     gene            complement(3974..4357)
                     /gene="rpsL"
                     /locus_tag="PKN80_03730"
     CDS             complement(3974..4357)
                     /gene="rpsL"
                     /locus_tag="PKN80_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNQ35156.1"
                     /translation="MPTISQLVRGARRQKSSRSKSPALALNPFKRGVCLYVKTMTPKK
                     PNSALRKIAKVRLSNQEEVIAYIPGVGHNLQEHSIVMVRGGRVKDLPGVRYHIVRGVY
                     DTLGVEGRRSSRSRYGTKVPKKQAE"
     gene            complement(4377..>8727)
                     /gene="rpoC"
                     /locus_tag="PKN80_03735"
     CDS             complement(4377..>8727)
                     /gene="rpoC"
                     /locus_tag="PKN80_03735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041857196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNQ35157.1"
                     /translation="GGRAQFGGQRFGEMEVWALEGYGAANMLQEMLTVKSDDIIGRKK
                     VYENIVKDRDFEDYQTPESFNVLINELKGLGFDIQTEARPENREAVTIRLASPEAIRK
                     WSHGEVKKPETINYRTFKAEKDGLFCEKIFGPVKDWECSCGKYKKVKYRGIVCDRCGV
                     EVTTSDVRRTRLGHIELVAPVSYVWIFKSVYNWMGTLLDMSPNALELVIYYDRYLVLE
                     PGTSGFKLKDLLSEKDYREALARSSGLKAEMGALAIRELLKKVDLNKEKAQLENELDR
                     NKGNQERRKNVIKKLKIVDGLLRTGTRPEWMISEVIPVIPPDLRPLLPLEGGRFATSD
                     LNDLYQRIINRNNRLKRLIKLHAPDIIIRNEKRMLQEAVDALLDNGRHGPQVLGRGNR
                     PLKSLADALRGKQGRFRQNLLGKRVDYSGRAVIVVGPELKLNECGLPKHMALELFAPF
                     ILRELRKREYVHTISSAKRALERNDPEVWEILEDVTRNHPVMLNRQPTLHRLSIQAFF
                     PRLTEGNVIRLHPLACTAFNADFDGDQMAVHVPLSLESQLEAQLIMNAATHLFSPASG
                     KPIVNLTREALLGCYYLTLKNEQLEAVKTVFSGPNEVLAAFDAGRLDYHQPIKVRIRR
                     QVIETTPGRVIFNELLPAELGYKNEQINGATLGRLIKDLYTRHGYEAAANFLDQAKEL
                     GFSIATRSGLTIGVRDLVIPEEKEVLIGLTEKDVRRIEKDYKDGAITEGERYHKVIDT
                     WTSCTNRLSENLFASLKNRLEKKFEINPIFLMVQSGARGNRQQVTQLMAMRGLMTRPT
                     KRITGGIGEIIEQPVRSNFREGLNVLEYFISTHGGRKGLVDTALKTSDAGYLSRRLVD
                     VCQDVVVTTEDCGTTAGIFVSPVWEGDRLMVPLRDRIVGRVAVDRIADLVTDEVVVEA
                     NEEINEGIAARIEEIGVEKIRIRSVLTCKAERGVCRKCYGWNLATLRMVELGEAVGIV
                     AAQSIGEPGTQLTLRTFHIGGTASRIVGETKVRARREGVMKFRNVRWVTNKNGQKVVL
                     NRDAILVMMDNRGREVDHYPAQIGDILKIDEETPVKRSDILMEHDPYMFSIVAEKSGQ
                     VHYDGLKAGVTYKDEVEDETAAGRKHKVKVIISHKEDITPVVVIRDSETKKALESHYL
                     PVDAHLMVREGESVRAGDILARTPRLVARVQDITGGLPRVAELFEARRPKNPAILAEI
                     DGSIVIEINERNQRVVKVVGTSGQERSYTIPFGKHLLVTENAYVQAGEKLTEGAVALD
                     DLLRIGGERKVFEYLLNEVQSVYRLEGVTINDKHIEAVIRQMLSRVRVTKAGDTLFSD
                     GQEVSRRRLLQENEKIAAEGGQPADWDALVQGITRVALSSESFISAASFQETIRVLTD
                     AALMGDEDPLQGIKENVILGRFIPAGTGTFRPARREAKREQPDFSVLDANLINPGALN
                     LDREEKPEEDKNQ"
BASE COUNT         1570 a         2780 c         2656 g         1721 t
ORIGIN      
        1 atcgggccgg cctcctggtc agcgcctacc acgaccagat tctgccgctg ttcaaggccc
       61 gttacttcca ttcgggggtg aatgtcacca tcggcctgcc cctgatccgg accagtcccg
      121 accatggaac cgccttcaat ctcgcccatc gcaacctggc ctccagcgcc agcatgcagg
      181 ccgcgatcag gctggcctta aagctggcct gccgcgctta gaagccatgg ccggactccg
      241 ccccaagaag aaattcggcc agcattttct cacccgtccc gtcctggccg cccggattgc
      301 cgaattcgcc ggcctcaagc ccggggatac agtggtggaa atcggcgccg gaaccggcct
      361 gctgacccgg gaactggccc ggcgggccgg ccgggtgctg acctttgaaa tcgaccggga
      421 actgctggcg gggctgaatt cggaactggc gcaacatccc aatgtggaag tgatgccggc
      481 cgatttcctg aaggccggac cggagcggct gaaaaccctg ggagaattca aggtggtggc
      541 caacctcccc tacggcgtgg ccaccccgat catattccag ctcctgaaat ttctccgccc
      601 ggaagcggga acggcttctc ttaaaacggc ggtgctcacc gtccagaagg aggttgcccg
      661 acggctgacc gccccggcgg gcgggcggga ctacggtccg ctcagcctgg cagtccaggc
      721 cggttcggaa gtggagtacc tgttgaccat caagccggga gccttccatc cgccgcccaa
      781 agtggactcg gccgtaatcc gtctccgtcc ccggagccat ccggccgccg agccggaaat
      841 actggaggcg gccctgaaac tggcgcgtac cgcctttcaa cagcggcgca agacgatttt
      901 aaacggcctg gccgccggac tgaaaagaag caaaaccgaa gccgccgcgc agctggccga
      961 ctgcggaatc ccttcgacgg cccgccctga agaactctcg ctggaggatt accttcggct
     1021 ggcgcgaacg gtaaaagaca ggggggtggc cggaaaaaat cgggcccggt caggttaggg
     1081 attggaaaga ggtttaatct tgacggcaaa atcccggttg accggcggca gatcgaattc
     1141 cagcctgccc tggcctttta cccgccgctc ctcaaaaatc acgccccggt aggtatccca
     1201 gaactccagc cggtagtcgc cggactccaa cccttccagg ctggccctgg cctgcttgaa
     1261 aaagggcagt ccgtcctcgg cgggcagccg ggacagctgc ccctggtcat aaaaccagat
     1321 aaaggcgcgc cggctgcctt tcaggcagaa actggccgtt ttttcaactc cttcgccatt
     1381 cagcgcgacc cgggccggaa ctaatttttc gagggagcga tcctccccgg ccgcgtaatt
     1441 ggccagcgcc cgataatgga agaagaggtt ctgctcttca atgaagttgt gccaccagaa
     1501 gagggggggc gccgcaaaag gcaggtggta accgatccag agcccgttgt gcagatccgc
     1561 cttcagcagg tcctcgtcgc cccccccgga agaaccgccg tattcggaga cgaaatgggg
     1621 cttgtcgaat ctctccagga accgggccgc gttagacaga gtggcggcca gaccgttccg
     1681 gccgccgtaa taggcgttgg tcagggtata ggccacctcg gggagcttga agacatcgct
     1741 gtcgtaaagg atggtgtaat ggccggtcac caggtggccc cagggatcgg tctccttgag
     1801 ataatcgatc atcacctggt accagtcgcg aaatccgggc gtctggtaga aggcgcccga
     1861 agcgcccacc aggttcatct cgttcagcaa ttcccaggca aagatgttgg ggctgtaacc
     1921 ccagcgtccc acgatgtagc gcaatcgctg ctggaaaagg cgccgggcct ccgggtcccg
     1981 gaagaaatcg tccggggaat cgagaaaacc gccgtttttc cggttgtagg ggttgtcgtt
     2041 ccactcctgg tcgcagaaag tgctgcagga gccgtggttg acgatcaaga gctggatgta
     2101 aatccccagc gcctcggagt gttccaggat ccaatccagt ttccaggcgt tcctgaggtt
     2161 gtaacggccg atgccctcgt agaagttgtt cggttcgtaa ttgctgtcct tcttccactc
     2221 cagagccagc caccaggcgg ccatccaggt ctcgaaaaaa ttctcgccgt tggcggccat
     2281 gcttttcagc cagtcttcca tggccagcgt tccctcggac gcggccaggg gcgcccggcg
     2341 aaaacccgcg tagcggttgt cgttcaagga acggatattg tggccgatcg ggtagaagga
     2401 ggtcccgtca tcgaaggcga agtggcgccg gtcgcgctgg ttgacccgga ggaagccccg
     2461 gcggccggag acaaccgcct cgaactcccg gaccggggaa cggaactttt cagcgccctt
     2521 atcgagagaa atctccagga aacagcggtg gcgaccgatt tcaaccgggc tgaaacgggc
     2581 cttccagacc gggacgccga ccgggatcag ccgatcctgg ctgttacgcc ccggaccttc
     2641 gaattcgtaa ccctggtaaa agaaggccgg caccacccat tcccgcccgg acggggccag
     2701 gaagtgggca tcgatccgga ccaggtccgt atcgaacgga ttctcaaagg agcggtccag
     2761 gccaaaagtc aattcgaaac gctcgtacag tccggtctgc ttccggtcct gctccaggaa
     2821 agtcagggtc cggccggcaa cccggaaatc atcaagaagg ataagccccg gcccaacccg
     2881 ggttccgaag aatttcaggc cgatgcgttc caccgaatac agcgccgaat cgctccaggt
     2941 ttcggaatgg ccgtgcggct gccaatcagt gctggaggag gtcaggtcat actccaggcg
     3001 gcgcgaagat ccggattgga gccggaagag acggctctgg taccagcgcc cgtcgccggt
     3061 cttcaagtaa accaggcacc ttatcccgcc ggacggcagc ctgggagccg cgatttcaaa
     3121 actcaggcgt tcgcaaaacc gccaatcttc atttagcggg gcccagattt ccgtttcacc
     3181 ggggaattct gcgctaatgc gcaggccggt gtctcccccg gctcttgcgg ccagcgagaa
     3241 agcgggggct tctccggtct cggggatttc cgcgcgccag ccctggcttg tgttccgaaa
     3301 atcaaataga acccggctgc cggcctgaag caacgatggc gcggccagcc aaccgacggc
     3361 cggcaggatc accagcagcc atgataaaat gcctctcaac cgggtatcag ccattttatt
     3421 ccaaccgttc aaaaaaaagc gggggaagtc ttcgactccc cccgccgatt ttttaacgca
     3481 cgcgcccaaa aaccttcacc agcgataatg agcgaaggcc cggttggcct cggccatccg
     3541 gtgcagttcg tcccgccgct tgatggtttc accttcaccc ttgtaggcat ccagaagttc
     3601 attggccagt ttctcgtaca tcggcttgcc cttcttcttc cgggaaacgg ccagcagcca
     3661 gcggatggcc agggccagcg accgggaaga gggaacctcg accggaacct ggtaggtggc
     3721 gccgccgatg cgccggctgc ggacctcgac ccggggccgg acattttcca gcgccttctt
     3781 gaaaacggcc aggggatttt ccttggtctt ttcgctcagg atgtccatcg acttgtagaa
     3841 actggcctgg caggtgctct tcttgccgct gtacatcatc atgttgatga acttggccgc
     3901 ctccaggctg ccgtacttgg gctccgggag cagttcccgc ttgattcgct ttcttcttct
     3961 tcccataggt caatcactcc gcttgttttt tcggaacctt ggttccatag cgactccggc
     4021 tgctgcgccg gccctccacg cccagggtat cgtaaacccc gcgcacgata tgataacgga
     4081 ccccgggcag gtccttgacc cggccgccgc ggaccatcac gatcgagtgt tcctggagat
     4141 tgtggcccac gccgggaatg taggcgatca cctcttcctg gttggaaagc cggaccttgg
     4201 cgatcttgcg cagcgccgaa ttcggcttct tgggggtcat ggtcttcacg taaaggcaga
     4261 caccccgctt gaacgggttc agggccagcg ccggtgactt tgaccgggaa ctcttctgcc
     4321 ttcgggcgcc gcgcactagc tgggaaatag tcggcatgct tcgctccttg actcggttac
     4381 tggtttttat cctcttccgg cttctcttcc ctgtccaggt tgagcgcgcc ggggttgatc
     4441 aggttggcat ccaggacgct gaagtcgggc tgttcccgct tcgcttcccg ccgggcgggc
     4501 cggaaggtac cggttccggc cggaatgaag cggcccagga taacgttttc cttgattccc
     4561 tggagcggat cttcgtcgcc catcagggcc gcgtcggtaa gcacccggat ggtctcctgg
     4621 aaggaggcgg ccgaaatgaa ggattcgctc gaaagggcca cccgggtgat gccctggacc
     4681 agggcatccc agtcggccgg ttgaccgccc tcggcggcaa tcttctcgtt ctcctggagc
     4741 aggcgccggc ggctcacctc ctggccgtcc gagaagagcg tgtcgccggc cttggtgacc
     4801 cttacccggg aaagcatctg ccggatgacc gcctcgatgt gcttgtcgtt gatggtaacg
     4861 ccttccaggc ggtaaaccga ctggacctcg ttcagcagat attcgaaaac cttgcgttcg
     4921 ccgccgatgc gcagcagatc gtccagagcc accgcgccct cggtcagctt ctcgccggcc
     4981 tggacgtaag cattctcggt caccagcagg tgcttgccga agggaatggt gtaactccgc
     5041 tcctggccgg aagtgccgac cactttcacc acccgctggt tacgttcgtt gatctcgatg
     5101 acgatcgaac cgtcgatctc ggccaggatg gccggattct tgggccggcg cgcctcgaaa
     5161 agctcggcca cgcgcggcag gccgccggtt atgtcctgta cccgggccac cagccgaggc
     5221 gtgcgggcca ggatatcgcc ggcccggacc gattcccctt cccggaccat caggtgggcg
     5281 tccaccggca ggtaatgcga ttcaagcgct ttcttggtct ctgaatcacg gatgaccact
     5341 accggggtga tatcctcctt gtggctgatg atcaccttga ccttgtgctt ccggccggcg
     5401 gcggtctcgt cctcaacctc gtccttgtaa gtaaccccgg ccttcaatcc gtcgtaatga
     5461 acctggccgc tcttctcggc cacgatcgaa aacatgtagg gatcgtgctc catcaggata
     5521 tcggaacgtt taaccggggt ctcctcatca atcttcaaaa tgtcgccgat ctgggccggg
     5581 taatggtcca cttcccgccc ccggttgtcc atcatgacca ggatggcgtc gcgattcagg
     5641 accaccttct ggccgttttt gttggtcacc cagcggacat ttctgaattt catgacgcct
     5701 tcccggcgcg cccgcacctt ggtttcaccc acgatccggc tggcggtgcc gccgatgtgg
     5761 aaggtgcgca gggtcaactg agtcccgggt tcgccgatcg actgggccgc cacgatgccg
     5821 accgcctcac ccagttcgac catccgcagg gtggccaggt tccagccgta gcatttgcgg
     5881 cagacgcccc gttcggcctt gcaggtcagc accgagcgga tccggatctt ttccaccccg
     5941 atctcctcga tccgggcggc gattccctcg ttaatctcct cgttggcctc gaccaccacc
     6001 tcgtcggtca ccaggtccgc gatccggtcg accgccaccc gtccgacgat gcggtcccgc
     6061 aggggaacca tcaggcggtc gccctcccag accggcgaaa cgaaaatgcc ggcggtggtg
     6121 ccgcagtcct cggtcgtgac caccacgtcc tggcagacat cgaccaggcg gcgggagaga
     6181 taaccggcgt cggaggtctt cagggcggtg tcgaccagcc ccttgcggcc gccgtgggtg
     6241 gagatgaaat actccagcac gttcaggcct tcccggaagt tggaccggac cggctgctcg
     6301 atgatttcac cgatgccgcc ggtgatccgc ttggtcggac gggtcatcag gccgcgcatg
     6361 gccatcagct gggtgacctg ctggcggttg ccgcgggcgc ccgactggac catcaggaag
     6421 atcgggttga tctcgaactt cttctcgagc cggttcttca gggaggcgaa caggttctcc
     6481 gagagccggt tggtgcagga agtccaggta tcgatgacct tgtggtaacg ttcgccctcg
     6541 gtgatggcgc cgtccttgta atccttctcg atgcgccgga cgtccttctc ggtcaggccg
     6601 atcaggactt ccttctcctc cgggatgacc aggtcgcgca cgccgatggt caaaccggaa
     6661 cgggtggcga tggaaaagcc cagttccttg gcctggtcga ggaagttagc cgccgcctcg
     6721 tagccgtgac gggtataaag atccttgatc agacggccca gggtggcccc gttgatctgc
     6781 tcgtttttgt agcccagctc ggccggcagc agctcgttga agatgacccg gcccggcgtg
     6841 gtctcgatga cctggcggcg gatccggacc ttgatcggct ggtggtaatc gagccggccg
     6901 gcgtcaaagg cggccaggac ttcgttcggc ccgctgaaga cggttttgac agcctccagc
     6961 tgctcgttct tgagcgtaag gtagtagcag cccagcagcg cttcccgggt caggttgacg
     7021 atcggcttgc cgctggccgg tgaaaaaaga tgggtggccg cgttcatgat cagctgggcc
     7081 tcgagctgcg actccaggga aagcggcacg tggaccgcca tctggtcgcc gtcgaagtcg
     7141 gcgttgaagg cggtgcaggc cagcgggtgc agccggatca cgttgccctc ggtcagccgc
     7201 ggaaagaagg cctggatgct gagccggtgc agggtcggct gccggttgag catgaccggg
     7261 tggttccggg tcacatcctc caggatctcc cagacctccg ggtcgttgcg ttccagggcc
     7321 cgcttggcgc tgctgatggt atgaacgtac tcgcgtttgc gcagttcgcg caggataaag
     7381 ggcgcgaaga gttccagggc catgtgcttg ggcaggccgc actcgttcag cttcaattcc
     7441 ggaccgacca cgatgaccgc ccggcccgag taatccaccc gcttgcccag caggttctgg
     7501 cggaaccgcc cctgcttgcc acgcagggcg tcggccagcg acttgagcgg ccggttgccc
     7561 cggcccagga cctgcgggcc gtggcggccg ttgtccagca gggcgtccac cgcctcctgg
     7621 agcatgcgct tctcgttgcg gatgatgatg tccggggcgt gcagcttgat cagccgtttt
     7681 aaccggttgt tgcggttgat gatccgctgg taaaggtcgt tcagatccga ggtcgcgaag
     7741 cggccgccct cgagcggcag caggggacgc aggtccggcg ggatgaccgg gatgacctcg
     7801 gagatcatcc attccggccg ggtgccggtg cgcagcaggc cgtcgacgat cttcagcttc
     7861 ttgatgacgt tcttccggcg ttcctggttg cctttgttcc ggtccagctc gttttccagc
     7921 tgggccttct ccttgttcag atcaaccttc ttgagcagtt cccggatcgc cagggcgccc
     7981 atctcggcct tcagcccgga gctccgggcc agggcctccc ggtagtcctt ctcgctgagc
     8041 agatccttga gcttgaatcc ggaagtgccc ggctccagca ccaggtagcg atcgtaatag
     8101 atgaccagtt ccagggcgtt gggcgacatg tccagcagag tgcccatcca gttgtaaacg
     8161 ctcttgaaaa tccagacgta gctgaccggg gccaccagct cgatatggcc cagccgggtc
     8221 cggcgcacgt ccgaggtggt cacctcgacg ccgcaccggt cgcagacgat gccccggtac
     8281 ttgaccttct tgtacttgcc gcaggagcac tcccagtcct tgaccggccc gaagatcttc
     8341 tcacagaaaa ggccgtcctt ctcggccttg aaggtccggt agttgatggt ctccggcttc
     8401 ttgacctcac cgtgcgacca tttgcggatg gcctccggcg aggccagccg gatggtcacc
     8461 gcctcccggt tttcaggccg ggcctcggtc tggatatcaa aacccagccc cttgagctcg
     8521 ttgatcagga cgttgaagga ctccggggtc tggtaatcct cgaaatcgcg gtccttgacg
     8581 atgttctcgt aaaccttctt gcggccgatg atgtcgtcgc tcttgaccgt cagcatctcc
     8641 tgcagcatgt tggccgcccc gtaaccctcc agggcccaga cctccatctc accgaagcgc
     8701 tggccgccga actgggcccg gccgccc
//
LOCUS       DAOQYN010000064         8669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8600_length_8669_cov_4.352333, whole genome shotgun sequence.
ACCESSION   DAOQYN010000064 DAOQYN010000000
VERSION     DAOQYN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8600_length_8669_cov_4.352333"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..466
                     /locus_tag="PKN80_03740"
     CDS             131..466
                     /locus_tag="PKN80_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ35158.1"
                     /translation="MDRLLRHPWPGNVRELKNIVERLLVLHADVPVLETAHLEGVLLG
                     PAVNPKLQPENKSLEEALQEYEKFLIGEAIKKSGGVKSRAAEILRTTRRILSYRMEKL
                     GLSNGSGSS"
     gene            complement(452..>689)
                     /locus_tag="PKN80_03745"
     CDS             complement(452..>689)
                     /locus_tag="PKN80_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35159.1"
                     /translation="LNRALRNRALRLDSGQVIPLKELLPGRNYRVRRLIRLILKREAG
                     RRVTDRELQVLLLRDYRVRLARRTVNLYRRSAGT"
     assembly_gap    690..699
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<700..1499)
                     /locus_tag="PKN80_03750"
     CDS             complement(<700..1499)
                     /locus_tag="PKN80_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35160.1"
                     /translation="MLNNAVSGRLSTRTGLLNKASLKLAAWIDLPESECLQQVRRLEA
                     DPVFQKMLSYRVLQRERFHGVSPAVFRLAPERDVPSFVSGFRGSEKEMAAVELIRKLG
                     RPDFEKFFLLPERLYTVAEISRATGLSSQEIGMLADALNNFTPGFYSPAELPGRRDPL
                     PGRPLTVVAGLTWSEGKPVLEDCVFDLCRGRYRLDPARLAELRQELTAEQRKRLSRIV
                     EEINWINRRRSVIYRVLKGVVEVQRKFLVSGDPDDLNGLTQRELRLSLG"
     gene            complement(1513..3768)
                     /locus_tag="PKN80_03755"
     CDS             complement(1513..3768)
                     /locus_tag="PKN80_03755"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNQ35161.1"
                     /translation="MQRRFTGLKANLALAGILSALFLSGSLERLELISLDFRFRHARA
                     YPPLSDSLAVVGITQGCVQSLGTSPDRSHYARLISFLSRSGAHSIISDIYFPHQGPAG
                     QDRELVAAVRSAGNVVLPVFSPEVLVPSGRPGAIHYRAGSLQSSFPELEAAAAGLGHI
                     NIHPDRDQVVRSAPAFIEYGDRTYPQLALAAVETARLLGREGGDGVKPPPLDRYGRYY
                     INHLPPDSFKQAGSIYAFSRVLQGQVPASAFRGRIVLIGQIIQGLKNADLAPTPFGYQ
                     FGALVEASALNTLLTGRVIKRAPPYWTVFGLFGLAVVLTLTIFSSRNRINLLVCLAAT
                     AAVFAGSFYVFKAGRLILETIPFCLLVVSSYGVSLLVSLQEAIALRRDSLAVLYREEE
                     EVSALIRPISAREIAEPSLADQNLNYSLNLRTPEMVMRIFSDSIGAGAVLIYDYRPEL
                     AGIRPLAASGFPEELAVELGEVVRRVGLDGKPWLVNRPAVLREPRLSKLADRTRNLMA
                     IPLVSGTEVSTVVVLMNKTPTAFSPTSNFTQHDVQLVTIMALQTIIAIQNAKLGLALK
                     DAHLDTILRLARAVEYRDRETGEHINRITDYVTMIAEGLKLSRLEVELIRNAMPMHDI
                     GKIGIPDAILLKAGPLTEEERRVMQTHTTIGAQILSKADSAILKASEIVAGSHHERFD
                     GKGYPKGLAGEAIPLYGRIAALADVFDAICSKRCYKEALPIETGIKVIREESGRMFDP
                     VLAEIFLKCFA"
     gene            complement(3804..4736)
                     /locus_tag="PKN80_03760"
     CDS             complement(3804..4736)
                     /locus_tag="PKN80_03760"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ35162.1"
                     /translation="MRSKIYKKSGFTLVELMIVIAIVVVLAIFGSTMLIKGRTAAKSI
                     ACLNNLRQIASALEMYRSDYDELAPTLYPTLISYLKNPEIFKCPVDAGTGQDSYGAFY
                     IARPSVDTQKLFLGCPRHQGQALALFGWPQFNVGRLATVLWNGTPVNQGTVVTGGRLN
                     FEDGTVVEIAPGMNVGVMLSCTDPGNRLYSTICIQEGDQGQISVNHHGTSQFEVVTPS
                     LITGVAGTKFNVLTSWGGALCSTKAECLEGTIYVEERGAVRYMSSVAAASSLEVKSRV
                     PLTSINSNTGYAVRTPRPWATRTRIPFGQVLRMP"
     gene            complement(4832..5563)
                     /locus_tag="PKN80_03765"
     CDS             complement(4832..5563)
                     /locus_tag="PKN80_03765"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNQ35163.1"
                     /translation="MKQQHPANDRGFTLLEILVVCIIILFLVMSVVAMIARVEEKARA
                     VKAKAEIIQLAMSIEKMKSDTGYFTLNLADLMRPVAPPGISKGWSGPYLESVVEKDPW
                     KEGYRIRLSANRSFSADYALGGGSSLFSWDTSFLTSTYLRIDNPDRRAITGGSLVLNG
                     VLLTGSINSASLPMLLPVSLAASNNQLSVTLAGDADAWIRVTIPAVVPARETYLITSY
                     GLDRQPGGKNLDGDITWHSDYQKFE"
     gene            complement(5560..6183)
                     /locus_tag="PKN80_03770"
     CDS             complement(5560..6183)
                     /locus_tag="PKN80_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35164.1"
                     /translation="MFGFDWNRKDRARHHYLVNKPMQLAYSGFLVWLVGVGMVIAWLI
                     TYYTIWNYIINGFPTAANLPAIIKQVNARLFWTLIIPGFLLLGIAGWSQIVLMHRIAG
                     PAYRIRKVMMDMAHGIWPSEVKLRNQDYLKEVADEINLFLNGQRKWAMDLEHQLRELD
                     EVLRESGAGQEEKQLADAQRQLEKVLRDLEAIFSVMNIDSGEKGAGS"
     gene            complement(6215..7468)
                     /locus_tag="PKN80_03775"
     CDS             complement(6215..7468)
                     /locus_tag="PKN80_03775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNQ35165.1"
                     /translation="MKEEKPVHRLPFYYSFAKFFSIIVVESVVILILAWTFPLETLGF
                     LVKVLFPFLFFIALFLIMYFLVLEHEAVKLLRAERRLERANQDFKILLKSHDRIFRRL
                     DSDPILKSGVAGLARLVPLRRAFLLVKGDDDSWQVVSRYHVTREDAETVTGFLLSGQG
                     PDLRGAGPENDGRERLEDGVKERPAGWQQLQLVPILNKSGGRFGFFVVDSEHLVHKFE
                     AVELLVKGVEGAIENALLHQRLKNLAIVDSLTNLYNHRYFHERLHEELNRAKRFSMPL
                     TLMISDIDDFKQYVDINGHSMADRTLKEIGGLVKENLRAIDIVARYGGDEFSYLLPQT
                     RAAGARVVAAKVKDACQAYEFTARAGHFHLTLSFGLAGCPDDGLDAEELLEKADRALF
                     LAKSRGKNAICLWEEAGTSTASPGA"
     gene            complement(7556..>8669)
                     /locus_tag="PKN80_03780"
     CDS             complement(7556..>8669)
                     /locus_tag="PKN80_03780"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNQ35166.1"
                     /translation="VACPGCGSCAGMFTANSMNCLTEGLGLGLPYNGTIPAVDSRRIR
                     LAKQAGMKAVSLARKGLKPREIATEAAFDNAIVLDMALGASTNTVLHLPAIAREAGLE
                     LPLKKFDRVSLRTPNLCKISPAGTHRMEDLDRAGGIPAVLAELAAAGLLDLSVKTAAQ
                     ETLGRIVARARNADPSIIRTAADPYRRQGGIAVLSGNLAVDGAVVKQSAVSESHLRHV
                     GPARVFEGEEAALKAVLENQIEDGSVIVIRQEGPRGGPGMREMLSVTSALVGAGRDER
                     VALLTDGRFSGGTRGLAIGHVSPEAAAGGAIALVHDGDIIEIDIVKRALKLQVSEAEL
                     KTRRAGWKPPRPKVRAGYLYRYSRSVSSAAQGAVLD"
BASE COUNT         1799 a         2677 c         2550 g         1633 t
ORIGIN      
        1 ccggccgtcg ttgcagcaga tcggacggca ggccgactgg aacgtccggc ggcacgttcg
       61 ggatcggctt cgcgccggcg agttcctcgt agaggtgttc gggcggctgt ccgagcaaca
      121 cgccgaggcg atggatcgcc tgcttcgcca tccgtggccg ggtaacgtcc gggaattgaa
      181 aaatatcgtc gagcgtctcc tggtccttca cgccgacgtg ccggtcctgg aaaccgccca
      241 cctcgaaggg gtcctgctcg gcccggcggt aaacccgaag ctgcagccgg aaaacaaatc
      301 cctggaggaa gccctgcagg agtatgaaaa atttttgatc ggagaggcta tcaagaagag
      361 cggcggcgta aaaagccggg cggcggaaat actcaggacc acccggcgca tattgagcta
      421 ccggatggaa aaactcggac tttcaaacgg ttcaggttcc agctgaacga cggtataaat
      481 tgacggtccg ccgggccagc cggacccggt aatcacgcag cagcagaact tgaagttccc
      541 ggtcggtcac tcttcggccg gcttcgcgct tgagaatcag ccggataagc cggcgcaccc
      601 ggtaattccg acccggcagc agttccttga gcggaatcac ctgaccggag tcgaggcgca
      661 gggcccggtt ccgcagggcc cggttcaggn nnnnnnnnnc ccagggaaag cctcagctcc
      721 cgctgggtta agccgtttaa atcatccgga tcgccgctga ccaggaattt ccgctgaact
      781 tccaccaccc ccttcaaaac ccggtaaatc acgctgcgcc gccggttaat ccagttgatc
      841 tcttccacga tccggctcaa tcgcttccgc tgctcggcgg tcaattcctg cctcaactcg
      901 gccaaacgag ccggatccag acgataccgc ccccggcaga ggtcgaagac acagtcctca
      961 agcaccggct tgccttcact ccaggtcaac ccggccacca ccgtcaaagg tcggcccggt
     1021 aaagggtccc gccgacccgg caactcggcg ggtgagtaaa aaccgggcgt aaaattgttc
     1081 aaggcgtcag ccagcatgcc gatttcctgg gaactcaacc cggtggccct ggaaatctcg
     1141 gccacggtat aaagacgttc cggcagcaga aaaaacttct cgaagtcggg ccgtcccagt
     1201 ttgcgtatca gttcgaccgc cgccatttcc ttttcggaac cccggaaacc cgaaacgaaa
     1261 gaggggacat ccctttccgg cgccagcctg aagaccgccg gcgaaacccc gtgaaacctt
     1321 tcgcgttgaa gcacccggta ggagagcatc ttctgaaaaa cggggtcggc ctccagccgg
     1381 cgtacctgtt gaaggcattc ggattcgggc aaatcgatcc aggcggccag tttcagtgag
     1441 gctttgttca gcaggccggt ccgggtcgac aaacgcccgg aaacagcatt attcagcaca
     1501 gtcaactccg actcaggcga aacacttcaa gaaaatctcg gccaggaccg gatcgaacat
     1561 ccgtccgctc tcttcccgga taactttgat cccggtttca atcggcagtg cctccttgta
     1621 gcagcgcttg gagcagatgg cgtcgaaaac atcggccaga gcggctattc ttccgtaaag
     1681 cggaatcgcc tcgccggcca ggcccttcgg atagcccttg ccgtcaaaac gctcgtggtg
     1741 actcccggcc acgatctccg aggccttgag aatggcggaa tcggccttgg acagaatctg
     1801 ggcgccgatg gtggtatggg tctgcatgac ccgccgctcc tcttcggtga gcggcccggc
     1861 cttcaagagg atggcgtccg gaatgccgat cttgccgatg tcgtgcatgg gcatggcgtt
     1921 gcgaatcagt tccacctcca ggcgggaaag tttcagcccc tcggcgatca tggttacgta
     1981 atcggtgatc cggttgatgt gttcgccggt ctcccggtcg cggtactcga ccgcccgggc
     2041 caggcggaga atggtgtcca ggtgcgcatc cttgagggcc agccccagtt tggcgttctg
     2101 gatggcgatg atcgtctgga gcgccatgat ggtcaccagc tgcacgtcgt gctgggtgaa
     2161 attgctggtg ggagaaaaag cggtcggcgt cttgttcatg agcaccacca cggttgagac
     2221 ctccgtcccc gacacgagcg gaatggccat caggttcctg gtccggtccg ccagcttcga
     2281 cagccgcggc tcccggagaa cggccggacg gtttaccagc cagggttttc cgtcgaggcc
     2341 gacccggcgc accacctcgc ccagttcaac ggccagttct tccggaaaac cgctggcggc
     2401 caggggcctg atcccggcca gttccggccg gtaatcgtag atcaggacgg ccccggcccc
     2461 gatcgaatcg gaaaagatcc gcatcaccat ctccggggtg cgcaggttca atgaataatt
     2521 caggttctgg tcggccaggg atggttcggc tatttcgcgg gccgagatcg ggcggatcaa
     2581 ggcgctgacc tcctcctctt cccggtagag cacggccagc gaatcgcgcc ggagggcgat
     2641 cgcctcctgc agcgagacca gcagggagac cccgtaggag gagaccacca gcaggcagaa
     2701 tgggatcgtt tccagaataa ggcggccggc cttgaaaacg tagaatgatc cggcaaaaac
     2761 agccgccgtg gccgccagac aaaccagcag gttaatccgg tttcggcttg aaaagatggt
     2821 cagagtcaac accacggcca gtccgaagag accgaaaacc gtccagtagg gcggcgctct
     2881 tttgataacc cggccggtaa gcagggtgtt gagagcgctc gcctccacca gcgctccaaa
     2941 ttgatatccg aagggcgttg gcgccaggtc ggcattcttc agcccctgaa tgatctggcc
     3001 gatcaacacg atccggcccc gaaaggccga cgccggaact tgtccctgga gtacccggga
     3061 aaaagcgtaa atggagccgg cttgcttgaa ggaatccggc ggcaggtgat tgatgtaata
     3121 gcggccgtag cgatccaggg ggggcggctt gaccccgtcc ccgccttccc gcccgagcag
     3181 ccgggcggtc tcgaccgcgg cgagagccag ttgaggataa gtccggtccc cgtattcgat
     3241 gaaggccgga gccgatcgaa ccacctggtc ccggtccggg tggatgttga tgtgacccag
     3301 accggccgcc gcggcttcca gttcagggaa actgctctga aggctcccgg cccggtaatg
     3361 aattgcgccc ggccgaccgc tgggaaccag aacctccggg gagaagaccg gaagaacaac
     3421 attgccggcg gaacgaaccg ccgccaccaa ttcccgatcc tgaccagccg ggccctggtg
     3481 cgggaaatag atgtcggaga tgatgctgtg cgccccggat cggctcagga aggagatcag
     3541 acgggcataa tgggagcggt cgggggaggt tcccaggctc tgcacgcacc cctgggtaat
     3601 gccaaccacc gccagcgagt ccgaaagggg agggtaggcc cgggcatgcc ggaagcgaaa
     3661 atcaaggctg atcaattcca gcctttccag actcccggac aggaataacg ccgaaagtat
     3721 cccggccaga gcaagattcg ccttaagccc ggtaaatcgg cgctgcatgg caagaggcgg
     3781 gccgcttcaa accgaagacg gcctcagggc atccggagaa cctgaccgaa gggtatgcga
     3841 gtcctcgttg cccaaggcct cggtgtcctt accgcataac cggtattgct gttgatggaa
     3901 gtgagcggga cgcggctttt cacctccagg ctgctggcgg cggccaccga gctcatatag
     3961 cggaccgcgc cgcgttcttc tacgtagatg gtgccctcga ggcactcggc cttggtcgaa
     4021 cagagagcgc cgccccagga agtcaggaca ttgaatttgg tcccggccac cccggtgatc
     4081 agcgaggggg taaccacttc gaactgggaa gtgccgtgat ggttgaccga aatctgccct
     4141 tgatcccctt cctggatgca gatcgtgctg tacaggcggt tcccgggatc agtgcaggag
     4201 agcatgacgc cgacattcat gcccggggcg atttccacca ccgttccatc ctcgaagttc
     4261 aaacggccgc ccgtgacaac cgttccctgg ttaaccggcg ttccgttcca gagcacggtg
     4321 gccaatcttc ccacgttgaa ctgcggccag ccgaaaaggg cgagcgcctg cccctggtgg
     4381 cggggacaac ccagaaagag cttctgggta tccaccgaag gccgggcgat gtaaaatgcc
     4441 ccataagagt cctgaccggt tccggcgtcg accggacatt tgaaaatctc cggattcttg
     4501 aggtaagaaa taagggtggg gtaaagtgtc ggggccagct catcgtaatc cgaacggtac
     4561 atttcgaggg ccgaggcaat ctggcgcagg ttattcaggc aggcgatgct cttggccgcc
     4621 gtgcgcccct tgatgagcat ggtgcttccg aagatggcca gcacaacaac gatggcaatg
     4681 acgatcatca attccactaa tgtaaaacct gactttttgt aaattttact gcgcatgagt
     4741 cacctctttt tttattggac gaataaaatt gttcgtctcc ttattcttgt accagttttt
     4801 aattgatttg tcaagtaaaa agcccgcccg ctcattcaaa cttctggtaa tcagagtgcc
     4861 aggtgatatc gccgtccaga ttcttgccgc ccggctggcg gtccaggccg taactcgtga
     4921 tcaggtatgt ttcccgggcc ggcaccaccg ccgggatcgt cacccttatc caggcatccg
     4981 cgtcgccggc cagggtcacc gaaagctggt tgttgctggc ggccaggctg accggcagca
     5041 gcatcggcag gctggccgag ttgatcgaac cggtcagaag cacgccgttc aaaacgagac
     5101 tgccaccggt tatggcgcgg cggtcgggat tgtcaatccg caggtaggtg gaggtcagaa
     5161 agctggtatc ccagctaaaa agactgctgc cgccgcccag ggcgtagtcg gctgaaaaac
     5221 tgcggttggc tgaaagtctg atccggtaac cctctttcca gggatccttt tcaaccacgc
     5281 tttcgaggta ggggccggac catccctttg agatgccggg cggggcgacc ggacgcatca
     5341 ggtccgcgag attgagtgta aagtaaccgg tatccgactt cattttttca atggacatcg
     5401 ccagctggat gatttcggcc ttagccttta ccgcccgggc cttctcctcg accctggcga
     5461 tcatggcgac cacgctcatt accaggaaga ggataatgat gcagaccacc agtatttcca
     5521 gcagggtgaa gccgcggtcg tttgccggat gttgttgttt cacgatccgg cccccttttc
     5581 tccgctgtcg atgttcataa ccgaaaaaat tgcttccagg tcgcgcagaa ccttctccag
     5641 ctgcctttga gcgtcggcca gctgcttttc ttcctggccc gcccccgatt ccctcaggac
     5701 ttcgtcaagc tcccgcagct gatgctccag gtccatggcc cacttccgct gtccgttcag
     5761 gaagagattg atctcgtcgg ccacctcctt gagataatcc tggttgcgga gcttgacctc
     5821 gctcggccag atgccgtggg ccatatccat catcaccttg cggatccggt aggccgggcc
     5881 ggcgatccgg tgcatcagca cgatctgact ccagccggca atccccagca gcagaaaacc
     5941 cgggatgatc agggtccaga aaagacgggc attgacctgc ttgataatgg ccggcaggtt
     6001 ggcggcggtc ggaaatccgt tgataatgta attccagatg gtgtaatagg ttatcaacca
     6061 ggcgatcacc atcccgacgc cgaccaacca gaccaggaac ccggaatagg ccagctgcat
     6121 cggcttgttg accagataat gatgacgtgc ccgatccttc cggttccagt caaatccgaa
     6181 catggctttt ctccttgtgg aattaatccg tatatcacgc ccccggggac gccgtagaag
     6241 taccggcttc ttcccagagg cagatggcat ttttaccccg gctcttggcc agaaacaagg
     6301 ctcggtcggc cttttccagc agttcctccg catccaatcc gtcatccgga cagccggcga
     6361 gtccaaagct gagcgtcagg tgaaaatggc ccgcccgggc cgtgaactca taagcctggc
     6421 aggcatcctt caccttggcg gccaccaccc gcgccccggc ggcccgggtc tggggcagca
     6481 ggtaggaaaa ctcgtctccg ccgtaccggg ccacaatgtc gatggcgcgc aggttttcct
     6541 tgaccagacc accgatttcc ttcaaggtgc ggtcggccat ggaatgaccg ttgatatcaa
     6601 cgtattgctt gaaatcatca atgtcggaaa tcatcagggt gagcggcatc gagaagcgct
     6661 tcgcccggtt cagctcctcg tgcagccgct cgtggaaata acgatggttg taaaggttgg
     6721 tcaggctgtc cacgatcgcc agattcttca gccgctggtg cagcaaggcg ttttcaatcg
     6781 ctccctcaac acccttaacc agaagttcaa ccgcttcaaa cttgtggaca aggtgctcgc
     6841 tgtccaccac gaagaatcca aaccggccgc cgctcttgtt caaaatgggt accagctgca
     6901 actgctgcca tccggccggc cgttctttca caccgtcttc cagccgttcc cggccatcat
     6961 tttccggacc ggcgcctctc aaatccggac cttggccgga tagcaggaat cccgtcaccg
     7021 tctcggcatc ctcccgggtc acgtggtaac gcgaaacaac ctgccagctg tcatcgtcgc
     7081 ccttgaccag cagaaaagcg cgccgaagcg ggaccagcct ggccagaccg gccactccgc
     7141 tcttcagaat cgggtccgaa tccagtcttc gaaagatccg gtcgtggctt ttgagcagaa
     7201 tcttgaaatc ctggttggcc ctttccagtc gccgttcggc ccgcaaaagc ttgacggcct
     7261 cgtgttccag aaccaggaag tacattatca gaaaaagggc gataaagaac aggaagggga
     7321 aaagcacctt gaccaggaaa ccgagcgtct ccaggggaaa agtccaggcc aggataagaa
     7381 ttacaaccga ctcaacaact attatggaaa aaaatttggc gaacgaataa taaaagggca
     7441 gccggtggac cggcttctct tctttcacgt tcaccccggc tttgattggg aaaaggccgt
     7501 cggcgccccc gccgggcgat gctgccggaa tacggcccgg ccggccgccc cgggctcaat
     7561 ccaggaccgc gccctgggcg gcggaactga ccgaacggct gtaacggtac agatacccgg
     7621 ccctgacctt cggacgaggc ggcttccagc cggcccggcg cgtcttcaac tcggcctcgg
     7681 aaacctgaag cttcagagcc ctcttgacaa tgtcaatttc aatgatatcg ccgtcgtgca
     7741 ccagggcgat ggcgcccccg gccgccgcct ccggtgaaac gtgtccgatg gccagccccc
     7801 gggtcccccc cgaaaagcgg ccgtcggtca gcagggccac ccgctcgtcc cggccggctc
     7861 cgaccagggc gctggtcacc gaaagcatct cccgcatgcc gggaccgccc cggggacctt
     7921 cctggcggat cacgataacg gagccatcct cgatctggtt ctccaggacc gccttcaagg
     7981 ccgcttcctc tccttcaaaa acccgggccg gaccgacatg cctcaggtga ctttcggaaa
     8041 ccgccgattg cttgaccacc gcgccgtcga cggccaggtt gccgctgagc accgctatgc
     8101 cgccctggcg gcggtaggga tcggccgcgg tccggataat gctcggatcg gcattgcggg
     8161 cgcgggcgac gatccggcca agcgtctcct gggctgccgt cttaaccgaa agatcaagca
     8221 ggccggccgc ggccaattcg gcaagcacgg ccgggatgcc gccggcccgg tccaggtctt
     8281 ccatgcgatg ggttccggcc ggggatatct tgcacagatt cggagtccga aggctgaccc
     8341 ggtcgaattt tttcaagggc agttccaggc ccgcctcccg ggcaatggcg ggaagatgca
     8401 aaacggtgtt ggtcgaagct cccagggcca tgtccaggac gatggcgttg tcgaaggcgg
     8461 cttcggtggc gatctcacgc ggtttcaggc ccttacgggc cagcgaaacc gctttcatgc
     8521 cggcctgctt ggccagacga atccgccggc tgtcgacggc cggaatggtc ccgttgtagg
     8581 gcaatcccag gcccagcccc tcggtcaggc agttcatgga attggccgtg aacatcccgg
     8641 cgcaggaacc gcaccccgga caggcaacc
//
LOCUS       DAOQYN010000065         8633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8648_length_8633_cov_4.071578, whole genome shotgun sequence.
ACCESSION   DAOQYN010000065 DAOQYN010000000
VERSION     DAOQYN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8648_length_8633_cov_4.071578"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PKN80_03785"
     CDS             complement(<1..244)
                     /locus_tag="PKN80_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNQ35167.1"
                     /translation="MSQLRRDPIINRWVIISTERARRPNDFRVEKQAIGKGGFCPFCE
                     GNEAKTPAEIMAIRRPDSGPNQPGWSLRVVSNKFPAL"
     gene            complement(270..1721)
                     /gene="gatA"
                     /locus_tag="PKN80_03790"
     CDS             complement(270..1721)
                     /gene="gatA"
                     /locus_tag="PKN80_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNQ35168.1"
                     /translation="MNLEKLTIAELGRLYRERRVSAVEAVAAYFKAIRAKDGLLHAYL
                     SVNESAALAQAEAVDRDRAAGAELPPLAGIPLAIKDNICLRGERTTCASRILENFVSP
                     YDATVIERLRGQRAIFLGKTNLDEFAFGSSTENSAFGVTRNPANPEYVSGGSSGGSAA
                     AVAAGLAAAALGSDTGGSIRQPASFCGVVGLKPTYGRVSRYGLVAFASSFDQIGPLTR
                     SVEDAAVILGAIAGPDPLDSTSVEAPVADYPAGLEAPVSGLRIGLPREYFGAGLDPGV
                     AEGVERGVKILEKLGASVREVSLPHTASAIAVYYVLAPAEASANLARFDGVRYGRRMP
                     EGATMRDLYELSRDAGFGKEVKRRIIMGTFVLSSGYYDAYYLQAQKVRTLIRQDFESA
                     FKEVDLLVTPTAPGPAFRIGSKVTDPLTMYLSDVFTVSCNLAGLPGLNLPVGRTAGNL
                     PVGLQLLGPVFSEELVLRAARALERELGGPGAV"
     gene            complement(1718..2440)
                     /gene="trmD"
                     /locus_tag="PKN80_03795"
     CDS             complement(1718..2440)
                     /gene="trmD"
                     /locus_tag="PKN80_03795"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNQ35169.1"
                     /translation="MRIDIITIFPEALKPLELSIIGRARSAGRLEIVLHDLRDFTTDR
                     HRKVDDKPFGGGVGMVMMAEPFYRAVNWVRAEAGPGQVVLLSPSGAVFNQARAAGLAA
                     AKHLILLCGHYEGVDARVREFCDLELSIGDYILTSGETAALVVVEAVARLLPGVLSAG
                     AAAEESFSQHLLEWPQYTRPADWRGRPVPPVLIGGHHREIAAWKLRAAEDATRRRRPD
                     LWRRYRAALKAAGHPPAAGKKK"
     gene            complement(2493..2735)
                     /gene="rpsP"
                     /locus_tag="PKN80_03800"
     CDS             complement(2493..2735)
                     /gene="rpsP"
                     /locus_tag="PKN80_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNQ35170.1"
                     /translation="MSTRIRLRRVGKKHQPNFRIVVIDDRKDAKGDYIELLGHYNNRM
                     QPKVLEINEERARYWIKVGAQPSDTVRSLLKQKGLL"
     gene            complement(2793..4055)
                     /gene="murA"
                     /locus_tag="PKN80_03805"
     CDS             complement(2793..4055)
                     /gene="murA"
                     /locus_tag="PKN80_03805"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNQ35171.1"
                     /translation="MDALKVTGGNRLSGGVRVSGSKNASLPILAATLLAEGRSRIYGV
                     PRLQDVRTMIKILTALGAEIDWSGDCLTVDTSGISNCTAPYELVKQMRASVCLLGPLL
                     ARFGRAAISLPGGCVIGDRPIDMHLKGLEKLSVRFTLSGGYVRGRAARLSGSSIFLGG
                     TYGSSVLATANVMMAACRAEGDTVIEFAACEPEIVNLADFLNRMGARIEGAGSHLVKI
                     QGVRRLRPTEFRILPDRIEAGTYLIAGALTRGQVTVENCVPEHLFALMEKLQEAGVTV
                     RHGLTTVEVQCRKRVRSVDIATLPYPGFPTDLQAQFMSLMVLADGTSLINENVFPERF
                     MHAAELKRLGADIILKDGTAIVRGRKRLTGTGVMASDLRASAALVLAGLAAEGETVVS
                     RIYHLDRGYENMETKLGALGAEVVRFSE"
     gene            complement(4043..5005)
                     /gene="prmC"
                     /locus_tag="PKN80_03810"
     CDS             complement(4043..5005)
                     /gene="prmC"
                     /locus_tag="PKN80_03810"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNQ35172.1"
                     /translation="MKRTSARSGRRPARAPKAEAVRGRPATFPRPPRAAGLLQWGAGR
                     LARAGIESPRRNAELLLAALLGCDRSGLYLEPERVVSRARAGRFREQIGRRGRGEPLQ
                     YLTGRVNFFRDSFRVRPGVFIPRPETELLVTQGLALLEAGRDGPATVLEVGAGSGAPG
                     LSLARHGRCRVWLLEKEARAVGLARLNRRRLGLDGRRVRILAGDWNDFHRRVRRRFQL
                     VVSNPPYVPAGQLSHLEREVRREPRTALAAGRAGLDFFRRLAPGAFRILVPGGWLLVE
                     IGFGQAAAVREIFRAAGFQTIGEFEDYNGRPRVIRGRKPFKWMP"
     gene            complement(4950..5228)
                     /locus_tag="PKN80_03815"
     CDS             complement(4950..5228)
                     /locus_tag="PKN80_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35173.1"
                     /translation="MEDKYSGLRKASSAWRIFAWGVAAVCMVGFLVGLGWIFRKPEAG
                     LVLCLVFAFHGLVGFVGCYTISQLILVALDIEKNIRAERPPAGPGSEG"
     gene            complement(5252..5623)
                     /locus_tag="PKN80_03820"
     CDS             complement(5252..5623)
                     /locus_tag="PKN80_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35174.1"
                     /translation="MNESSTGLNENLVGMLCYVGLVFTGLFFFLTEKKSKYVRFHGLQ
                     STLFFVAWVIVWMAFGSIFSFLPVVADWLLRLVKVAGLVGWVLLIVKSYKGEYFLLPV
                     VGRIAMENSEFKTPESQDKQA"
     gene            complement(5620..6702)
                     /gene="prfA"
                     /locus_tag="PKN80_03825"
     CDS             complement(5620..6702)
                     /gene="prfA"
                     /locus_tag="PKN80_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNQ35175.1"
                     /translation="MAARSPELNLTELQARFQDLSLQLSRSESGLSNGERERLLEELR
                     RLNELIPLQQKLERTRKERAGAAELLAGETCSQLAELAREEVDRLDREVESLEKDLVG
                     RLLEDPRDQRNAIVEIRAGTGGEEAALFARDLFRMYSRFAERHGFKLEVFDTSATEKN
                     GLKSVIFMVRGAGAYGRFKYESGVHRVQRVPETEASGRIHTSAATVAVFPEIRIQEVR
                     IENKDLRIDTFCASGHGGQSVNTTYSAVRITHLPTGLVVSCQDERSQLQNKERALKIL
                     AARLAEREKRSQEESLSVERRALIKTGDRSEKVRTYNFPQNRLTDHRINFSVHNLKEI
                     LDGNLEPVLEALVDYELQLRNKGEGS"
     gene            complement(6683..6889)
                     /gene="rpmE"
                     /locus_tag="PKN80_03830"
     CDS             complement(6683..6889)
                     /gene="rpmE"
                     /locus_tag="PKN80_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNQ35176.1"
                     /translation="MKKGIHPQYFPAKVICACGNVVETGATKKEMHVEICAACHPFFT
                     GKTKFVDTAGRVEKFRRRYGSPKS"
     gene            complement(6886..7440)
                     /locus_tag="PKN80_03835"
     CDS             complement(6886..7440)
                     /locus_tag="PKN80_03835"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNQ35177.1"
                     /translation="MGAVRANAARKIFASLVLVLLLAALFFLFYRPPLNFLSQQPLEL
                     YFSLVSEEGETVSPVTRPVPRRWKTEERLEQAIRLLLAGPDETEKSAGYETELPTGAE
                     LISCWVEGDLAFLNFSPRVEDGGGTASMRNRLRQIVFTATQFPGINRVRFLIDGETIK
                     YFSSEGLTEVENPLGRSDFEKEEE"
     gene            complement(7473..>8633)
                     /locus_tag="PKN80_03840"
     CDS             complement(7473..>8633)
                     /locus_tag="PKN80_03840"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNQ35178.1"
                     /translation="NALLGKKCFSLRIASWLARSENWLAEHMIIIGIEDPRGRITYVA
                     AALPSACGKTNLAMLESTLPGYRIWTLGDDIAWLHVGPDGRLYALNPEAGFFGVAPGT
                     SLRTNPNMVKTLQAGRFYPTLFTNTGLDVSRNAPWWEGLDETPNPRLDWQGRPWKEGA
                     GPAAHPNARFTVSAQNCPTLSPEYDNPAGVPISAIILGGRRTSVAPLVLQSRDWLEGV
                     CLGSIMGSETTAAAIHQVGQLRRDPMAMLPFCGYNLGDYLGHWLRMGESLKEPPAIFF
                     VNWFRKDGGGDFLWPGFRENIRVMKWIIERTAGGQGNGAAETPIGLVPERSAVNLDGL
                     DLAPGAWKELTAVDRNEWRAEITTGREFLAQFGDRLPTRVREGLDRLDRNLG"
BASE COUNT         1481 a         2938 c         2790 g         1424 t
ORIGIN      
        1 ccagcgccgg gaacttgttg gagaccaccc gcagcgacca gcccggctgg ttggggccgg
       61 aatccggccg gcggatcgcc atgatctcgg ccggggtctt ggcctcgttt ccctcgcaga
      121 aggggcagaa gccgcccttg ccgatggcct gcttttcaac ccggaaatcg ttcggacgcc
      181 gggccctctc ggtggaaatg atcacccagc ggttgatgat cggatctctt ctcaattgcg
      241 acattatggc atctccaggg tgtgaggggt caaaccgcgc ccgggccgcc caattcgcgc
      301 tccagggccc gggccgctcg caagaccagt tcctccgaaa agaccggccc gaggagctgg
      361 agtccgaccg gcaggttgcc ggccgtccgg cccaccggca ggttgaggcc gggcagaccg
      421 gccaggttgc aggaaaccgt gaacacgtcc gaaagataca tggtgagcgg gtcggtcacc
      481 tttgacccga tccggaaggc cggccccggg gcggtgggcg tcaccagcag gtccacttcc
      541 ttgaaggccg actcgaaatc ctgccggatg agggtccgca ccttctgggc ctgtaggtaa
      601 taggcgtcgt agtacccgct ggaaagcacg aaggtcccca tgatgatccg ccgtttcacc
      661 tctttcccga atccggcgtc ccggcttaac tcgtaaagat cgcgcatggt cgcgccctcc
      721 ggcatccggc ggccgtagcg gacgccgtcg aagcgggcca ggttggcgct ggcctcggcg
      781 ggcgccagca cgtagtaaac cgcgatggcc gaagcggtat ggggaagcga gacctcccgg
      841 acggaggcgc ccagcttctc caggatttta acgccccgct ccactccctc ggccactccc
      901 gggtcgaggc cggcgccgaa gtattccctg ggcagcccga tccggagtcc ggagaccggc
      961 gcctccagcc cggccgggta atcggccacc ggcgcttcga ccgaggtgga atcgagcggg
     1021 tcgggaccgg caatcgcgcc caggatgacg gcggcatcct cgacgctccg ggtcagaggt
     1081 ccgatctggt cgaaggagga ggcgaaggcc accaggccat atcgggaaac gcggccatag
     1141 gttggtttca gtcccaccac tccgcagaag gaggccggct ggcggatgga accgccggta
     1201 tcggaaccga gcgcggccgc ggccagcccg gcggccaccg cagcggccga tccgccgctg
     1261 cttccgcccg aaacgtattc cgggttggcc gggttacggg tgacgccgaa ggccgaattc
     1321 tcggtcgagg aaccgaaggc gaattcgtca aggttggtct tgcccaggaa gatcgcccgc
     1381 tgtccgcgca gccgctcgat gacggtcgcg tcgtaaggcg agacgaaatt ctcaaggatg
     1441 cgcgaggcgc aggtggtccg ctcgccccgc aggcagatgt tgtccttgat ggccagcgga
     1501 atcccggcca gcggcggcag ttcggccccg gcggcccggt cgcggtcaac cgcctcggcc
     1561 tgggccagcg ccgccgattc gttgaccgaa aggtaggcgt gcagaagccc gtccttcgcc
     1621 cggatggcct tgaaataggc ggcgaccgcc tcgacggccg acacccggcg ctcccggtaa
     1681 aggcggccca attcggcgat ggtcagtttt tcgaggttca tttcttcttt cccgcggcgg
     1741 gcggatggcc ggccgccttg agggcggccc ggtaccgcct ccacaggtcg ggccggcgcc
     1801 gacgcgtcgc gtcctcggcc gcccgcagtt tccaggcggc gatttcgcgg tggtggccgc
     1861 cgatcagcac cggtggtacc gggcggccgc gccagtcggc cggcctggtg tactggggcc
     1921 attccaggag atgctggctg aaggattctt cggcggccgc cccggccgac aggaccccgg
     1981 gcagcaggcg ggcgaccgcc tcaaccacca ccagggcggc cgtctcgccg ctggtcagga
     2041 tgtaatcacc tatcgagagt tccaggtcgc agaattcgcg cacccgggca tccactccct
     2101 cgtagtgtcc gcagagcagg atcaggtgct tcgcggcggc cagcccggcc gcccgggcct
     2161 ggttgaaaac cgcgccggag ggactcagga ggacgacctg gcccggcccc gcctcggccc
     2221 ggacccagtt gaccgcccgg taaaagggct cggccatcat caccatgccc accccgccgc
     2281 cgaagggctt gtcgtcgacc ttgcggtgac ggtcggtggt gaaatcacgc aggtcgtgca
     2341 gaacgatctc caagcggccg gccgaacggg cccggccgat gatgctgagt tccaggggct
     2401 tcaaggcctc cggaaaaatc gtgattatgt cgattctcat gcgccccggc gccggccgga
     2461 aaggcggccc cggctacggc cgcccgcggg gttcagagca gacccttctg cttcagcagg
     2521 gagcgcacgg tatcgctcgg ctgggcgccg actttgatcc agtaccgggc ccgctcctcg
     2581 ttgatttcca gcaccttggg ctgcatccgg ttgttgtagt ggccgagcag ctcgatgtaa
     2641 tctcccttgg catccttgcg gtcgtcgatc accacgatcc ggaaattcgg ctggtgcttc
     2701 ttaccgaccc gtcttaacct gattctggtg gacatgcttc cgcctccttg ttatccgttg
     2761 acgtttctat tatcgatgac gcccgaggcg tcttattcgc tgaaacggac gacctcggcc
     2821 ccgagggcgc cgagcttcgt ctccatgttc tcgtaaccgc ggtccaggtg ataaatgcgg
     2881 ctgacgaccg tctcgccctc ggcggccagg ccggccagga ccagggcggc gctggcccgc
     2941 aggtcggagg ccatcactcc ggtcccggtc aggcgtttcc ggccgcgcac gatggcggtg
     3001 ccatccttca ggatgatgtc ggcgccgagc cgcttcaatt cggccgcgtg catgaacctt
     3061 tcgggaaaaa cgttctcgtt gatgaggctg gtcccgtcgg ccaggaccat cagcgacatg
     3121 aactgggcct gcaggtcggt cgggaagccc gggtacggca gggtggcgat gtcgacgctg
     3181 cgcacccgtt tgcggcactg gacctcgacc gtcgtgagcc cgtggcggac ggtcacgccc
     3241 gcctcctgga gcttctccat cagcgcgaag agatgctccg gcacgcaatt ctccaccgtc
     3301 acctgccccc gggtcaaggc gccggcgatc aggtaggtgc cggcctcaat gcgatccggc
     3361 aggatccgga attccgtcgg ccgcagccgc cggaccccct ggattttaac caggtggctg
     3421 ccggctccct cgatccgggc ccccatccgg ttcaggaaat cggccaggtt gacgatctcc
     3481 ggttcgcagg cggcgaactc gatgaccgtg tcgccctcgg cccggcaggc ggccatcatc
     3541 acgttggcgg tggccagaac gctggaacca taggtgccgc cgagaaaaat tgaggatccc
     3601 gaaagacggg cggcccgccc ccggacgtac ccgcccgaaa gggtgaaccg gaccgaaagt
     3661 ttctcgaggc ccttgaggtg catgtcgatc ggccggtcgc cgataacgca gccgcccggc
     3721 agggagatgg cggcccggcc gaagcgggcc aggagcggtc ccagcaggca gaccgaggcc
     3781 cgcatctgct tgaccagctc gtagggggcg gtgcagttcg agatgccgga agtgtcgacc
     3841 gtcaggcagt cgccgctcca atcgatctcg gcccccaggg cggtcaggat cttgatcatg
     3901 gtccgcacgt cctggaggcg cggcaccccg taaatacggc tccggccctc ggccaggagc
     3961 gtggccgcca gtatcggcag gctggcgttc ttggaaccgc tgactcgaac cccgccggac
     4021 agtcggtttc ccccggttac tttcaaggca tccatttaaa aggtttccgg ccgcggatta
     4081 cccgcggccg accgttgtaa tcctcgaact ccccgatagt ctgaaagccc gctgcgcgaa
     4141 aaatctcccg gaccgcggcc gcctggccga aaccgatctc gaccagcagc cagccgcccg
     4201 gcaccagaat gcggaaggcc cccggcgcca gccggcggaa aaagtccagc ccggcccggc
     4261 cggccgccag ggcggtccgg ggctcgcgcc ggacttcccg ctcgagatgg gaaagctgcc
     4321 ccgccggtac gtagggcggg ttggagacca ccagctgaaa gcggcggcgg acccgccggt
     4381 gaaaatcgtt ccagtctccg gccaggatgc ggacccgccg gccgtccagg cccagccggc
     4441 gccggttaag ccgcgccagg ccgaccgccc gcgcctcctt ctcgagaagc cagacccggc
     4501 accggccgtg cctggccagg gaaagtcccg gcgcgccgct gccggcgccc acttcgagaa
     4561 ccgtggccgg cccgtcccgg cccgcttcca gcagggcaag cccctgcgtg accagcagtt
     4621 ccgtctccgg ccgggggatg aagaccccgg gccggacccg gaaagaatcc cggaaaaaat
     4681 tgacccggcc ggtcaggtac tggagcggct cgccccggcc gcggcggccg atctgctccc
     4741 ggaaccgccc ggcccgcgcc cgggaaacga cccgttccgg ctccaggtaa agcccggacc
     4801 ggtcacagcc cagcagcgcc gccagcagca actcggcatt gcggcggggc gactcgatcc
     4861 ccgcccgcgc caggcggccg gcgccccatt ggagcaggcc ggcggcccgc ggcggccgcg
     4921 ggaacgtcgc gggccggccg cggacggcct cagccttcgg agcccgggcc ggccggcggc
     4981 cgctccgcgc ggatgttctt ttcaatgtcc agcgccacca ggatcagctg gctgatcgtg
     5041 tagcagccca caaaaccgac caggccgtgg aaggcgaaga ccaggcacag gaccagtccg
     5101 gcctcgggct tgcggaagat ccagcccagc ccgaccagga acccgaccat gcagacggcg
     5161 gccacgcccc aggcaaagat ccgccaggcg gaagaggcct tgcgcaaacc cgagtacttg
     5221 tcttccatgt cttccacggt tcgaggtgaa ttcaagcctg cttatcctgg ctttccggag
     5281 tcttgaactc ggagttttcc atggcgatcc ggccgaccac cggcagcagg aaatactcgc
     5341 ccttgtatga cttgactatc aacaggaccc aacccaccag gccggccacc ttcaccagcc
     5401 gcagcagcca gtcggccacc accggcagaa aggaaaagat gcttccgaag gccatccaga
     5461 caatgaccca ggcaacgaaa aagagcgttg actgcaggcc gtgaaaacgg acgtacttgc
     5521 tcttcttctc ggtcaggaag aagaaaaggc cggtaaagac cagcccgacg tagcagagca
     5581 tgccgaccag gttttcgtta agaccggtgc tgctttcgtt catgagccct cccccttatt
     5641 ccggagctgc agttcataat caaccagggc ctccagcacc ggttccaggt tgccgtccag
     5701 gatctccttc aggttgtgca ccgaaaagtt gatccggtgg tcagtcagcc ggttctgcgg
     5761 aaaattgtag gtccgcacct tttcgctccg gtcgccggtc ttgatcaggg cgcgccgctc
     5821 gaccgacagg gactcctcct gactcctctt ctcccgctcg gccagccggg ccgccagaat
     5881 cttcagggcc cgctccttgt tctgaagctg gctccgctcg tcctggcagg agaccaccag
     5941 gccggtgggc aggtgagtga tgcggaccgc cgagtaggtg gtgttgaccg attggccgcc
     6001 gtggccggaa gcgcagaagg tgtcgatgcg caggtccttg ttttcgatcc gcacttcctg
     6061 gatccggatc tcggggaaga ccgccacggt ggccgccgag gtgtggatgc ggccgctcgc
     6121 ctcggtctcc ggcacccgct ggacgcggtg gacgccgctc tcgtacttga aacgcccgta
     6181 ggccccggcc ccgcgtacca tgaagatgac cgacttgagg ccgttctttt cggtggcgga
     6241 ggtgtcaaag acctccagct tgaaaccgtg gcgctccgca aaacggctgt acatccggaa
     6301 caagtcgcgg gcgaagagag cggcctcctc gccgccggtg ccggcccgga tttcgacaat
     6361 cgcgttgcgc tggtcacgcg gatcttcgag aagacgccca accaggtcct tctcgagcga
     6421 ctccacctcc cggtcgagcc gatccacttc ctcgcgcgcc agttcggcca gctgggaaca
     6481 ggtttccccg gccagcagtt cggccgcccc ggcccgctcc ttccgggtcc gctccagttt
     6541 ctgctggagc gggatcaact cattcagacg ccgcaactct tccagcagcc gctcccgttc
     6601 gccgttgctg aggccgcttt cggagcggct caactgcaga ctgaggtcct gaaacctggc
     6661 ctggagttcg gtaaggttga gctcaggact tcgggctgcc atagcggcgc cggaacttct
     6721 ccacgcgtcc ggcggtatcg acgaacttgg tcttgccggt gaagaaggga tggcaggcgg
     6781 cgcagatctc cacgtgcatc tccttcttgg tcgccccggt ctcaacgaca ttgccgcagg
     6841 cgcagatgac cttggccggg aaatactggg ggtggattcc cttcttcatt cttcctcctt
     6901 ctcgaaatcg gaacggccga gggggttctc gacctcggtc agtccctcgc tgctgaagta
     6961 cttgatcgtc tcgccgtcaa tcaggaaccg cacccggttg atgccgggaa actgcgtggc
     7021 ggtaaaaacg atctggcgca accggttccg catgctcgcg gtcccgccgc catcctcgac
     7081 ccggggtgaa aaattcagga aggcaaggtc gccttcaacc cagcaactta tcaattcggc
     7141 cccggtcggg agctcggttt cgtaaccggc gctcttctcg gtttcgtccg gcccggccag
     7201 caaaagccgg atggcctgct cgagtcgttc ctcggtcttc cagcgccggg gaaccggccg
     7261 ggtgaccggg gagaccgtct cgccctcctc ggagaccagg ctgaaataga gttccagcgg
     7321 ctgctggctc aaaaaattca acggcggccg gtagaagaga aaaaacagcg cggccagcag
     7381 gagcaccagc accagcgaag cgaaaatctt tcgggccgcg ttcgccctga ctgcgcccat
     7441 cccgccaccc cccttggttg gcccggacaa gttcacccta aattgcggtc caggcgatca
     7501 agcccttccc gaacccgggt cggaagccgg tccccgaact gggccaggaa ttcccggcca
     7561 gtcgtaattt cggcgcgcca ttcattccgg tcaaccgccg tcagttcctt ccaggcgccc
     7621 ggggccaggt caagcccgtc aaggttgacg gcgctccgct ccggcaccag accgatcggc
     7681 gtctccgccg caccgttgcc ctgcccgccg gcggtccgct cgatgatcca tttcataacc
     7741 cggatattct cccggaagcc gggccagagg aaatcgccgc cgccgtcctt gcggaaccag
     7801 ttgacgaaga agatcgccgg cggttccttc agggattcgc ccatcctgag ccagtggccc
     7861 aggtaatcgc ccaggttgta accgcagaaa ggcagcatgg ccatggggtc gcgccgcagc
     7921 tggcccacct ggtgaatggc ggccgcggtc gtttccgaac ccatgatcga acccaggcag
     7981 accccctcca gccagtcgcg gctctggagg accaggggcg ccacgctggt gcgccggccg
     8041 cccaggatga tggccgaaat cgggacgccg gccgggttgt cgtactcggg tgaaagggtc
     8101 gggcagtttt gggccgaaac cgtgaaacgg gcgttcggat gggctgccgg tcccgccccc
     8161 tccttccagg ggcgcccctg ccagtccagc cggggattgg gcgtctcatc cagaccttcc
     8221 caccagggcg cgttgcggct gacatccagg ccggtgttgg taaagagagt cgggtaaaaa
     8281 cgtcccgcct ggagagtttt gaccatgttg ggattggtcc gcagcgaagt gccgggcgcc
     8341 accccgaaga agccggcctc cgggttgagg gcgtaaagac gaccgtccgg cccgacatgc
     8401 agccaggcga tgtcgtcccc cagagtccag atccggtagc ccggcagggt ggattcgagc
     8461 atggccaggt tggtcttgcc gcaggccgag ggcagggccg ccgccacgta agtaatccgg
     8521 ccccggggat cctcgatgcc gatgatgatc atgtgttcgg ccagccagtt ctcgctccgg
     8581 gccagccagg aggcgatccg cagggagaag catttcttgc ccagcagggc gtt
//
LOCUS       DAOQYN010000066         8631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8655_length_8631_cov_4.310984, whole genome shotgun sequence.
ACCESSION   DAOQYN010000066 DAOQYN010000000
VERSION     DAOQYN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8655_length_8631_cov_4.310984"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PKN80_03845"
     CDS             complement(<1..672)
                     /locus_tag="PKN80_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35179.1"
                     /translation="MKRWAMLFGLVGLAVVLSGCMEIENRYYIYPERNGKQTVLITMD
                     PVNMTGALVGLAGSFGGGQPGGGGLPGGENPVQQLEKMGISGLSDIRTTFSVYYDDIL
                     NLAGKSKEIPVGAKEIVWEKNKEGLYHFRMVLDMSQMKKGMGMGEGGQPGGGPGPMGM
                     LAMMQGVKVHTVLVMPGRVVRSNGQNKGREARWSSSLQDLLNTGELVYEAYSDTAVLP
                     EMEAEL"
     gene            complement(669..1835)
                     /gene="ispG"
                     /locus_tag="PKN80_03850"
                     /gene_synonym="gcpE"
     CDS             complement(669..1835)
                     /gene="ispG"
                     /locus_tag="PKN80_03850"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNQ35180.1"
                     /translation="MSIKSTVARPVRIGRLTVGGGAPVRVQGMTKTRTADLKATRLEL
                     EALAAAGAELVRLAVKDEEDLAAFARLKSISRLPLVADIHFRGDLALGALAAGADKVR
                     LNPGNLDDPVRLAEIIRLARRKGAAIRIGVNSGSLPGGPGEPVSGLETARLMVRTAAA
                     YLENFETLDFHNLVVSLKSSSVPVTLAANRLFRRHFDYPLHLGVTEAGRGGPALVKSS
                     IGIGALLADGIGETLRVSLSGPAVEEVAAGRWILQALELRNFQPELISCPTCGRCRSD
                     LFSILDRVEDGLKRLPGYPERYAGFKVAVMGCEVNGPGEAAAADIGVACGGKRGLLFR
                     KGRVVERPPAAGLAEALLAELSRLNPGPVKRESNGDHPIKTKPKRGGTSNRRRK"
     gene            complement(1883..2962)
                     /gene="rseP"
                     /locus_tag="PKN80_03855"
     CDS             complement(1883..2962)
                     /gene="rseP"
                     /locus_tag="PKN80_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNQ35181.1"
                     /translation="MNAILSFLVNLFSVVLVFGILIISHELGHFWAARRAGVRVELFT
                     VGFGPKLFGFQRGGTQYQVSLIPFGGAVKLAGEENYGRATPKGDEYFAQPAGKRMLIL
                     VMGSVHNLLLGFLAFVLVFLLGVELANYREPVIGGLEPGYPAVQAGLQAGDRIIRVAG
                     VPVKDWPSLSGEIHRYPDREISLEVDRAGRVFQVRVTPRAETLEAGQGSRRIGLVGIG
                     PQIQREKYGLFAALGRAGKATAGVVHDIFYYLGRIFTRKMSGRDLAGPLGIAQLSTRF
                     FRLGWSNFLYFLALLSVNLAVVNLFPLPVFDGGHLFGVMLERISGRKPSPRFIEVGQM
                     IGLGLVLALAVFVTYNDLLRIIVGR"
     gene            complement(3006..3977)
                     /locus_tag="PKN80_03860"
     CDS             complement(3006..3977)
                     /locus_tag="PKN80_03860"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNQ35182.1"
                     /translation="MRYLITGGAGFIGSHLTEHLLPAGHRVTVLDDLSTGRLKNLEAV
                     KHHPNLQILVDSILNRPVLERLVGEADAVFHLAAAVGVRTVIEQPLQSLRTNVEGTCN
                     LLEACAAARRKIVLASTSEVYGKNENVPFAEENDIVLGATTIRRWAYACSKALDEFFA
                     LAYAAEAGLPVIIVRLFNTIGPRQTGRYGMVVPRFVRQALEGRPITIYGDGRQSRCFT
                     CVSDVIEALGRLVESPAAVGGVFNIGSREEVTIEDLARRIRTLCKSRSPLVHLDPEQD
                     YKEGFEDMRRRVPDLTRLEATIGYRPRVSLETALKQIIADFKAGRED"
     gene            complement(3981..5240)
                     /gene="ahcY"
                     /locus_tag="PKN80_03865"
     CDS             complement(3981..5240)
                     /gene="ahcY"
                     /locus_tag="PKN80_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNQ35183.1"
                     /translation="MAEFEIRDRGLVDEGVNRVEWAGREMPVLLSIRERFAQSHPFAG
                     LRLGGCLHVTSETANLALALQAGGAEVFLCASNPLSTNDAVAAALAVKYGIHVHAVRG
                     EDRDAYYRHLEAVLSARPSVLLDDGADLISQARRRDEAFCREVFGGTEETTTGVIRFR
                     NLAREGRLRFPIVAVNEADTKHLFDNRYGTGQSTIDGLLRATNLLLAGRKVVVAGYGW
                     CSRGIAQRARGLGAIVAVTEIEPVRALEALMDGFLVMPSLEAARFGDVFITATGNRSI
                     FRAEHFRVMKSGAILANSGHFDVEIDIPALAGLSRARRLIRDQVEEFELSDGRRLYLL
                     AQGRLLNLAAAEGHPSAVMDMSFANQALSAEHLVKNRGRIKPGLYPVPEEIDREVARL
                     KLAASGVEIDRLTPDQEKYLSSWQEGT"
     gene            complement(5546..6178)
                     /locus_tag="PKN80_03870"
     CDS             complement(5546..6178)
                     /locus_tag="PKN80_03870"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HNQ35184.1"
                     /translation="MKILISAGPTREWIDPVRYISNPATGRLGYLLAEEAVRRGHEAV
                     LVCGPTGLENPAGVKVVRVETARQMETALRRHFPDCQALFMTAAVGDWRPRFRRTKLK
                     CKRKFSLTLFPNPDILAGCGRLKKNGQTLIGCALETNNLVEAGRAKLRRKNLDFILVN
                     EPGFFGKEKGRHQSFLLSRRGEVYDLSNRSKEDISRFLYDRCLLTCSNPS"
     gene            complement(6175..7356)
                     /gene="ftsZ"
                     /locus_tag="PKN80_03875"
     CDS             complement(6175..7356)
                     /gene="ftsZ"
                     /locus_tag="PKN80_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNQ35185.1"
                     /translation="MMRIRPEEQNMGAVKIKVVGVGGAGGNVVSRMVERLSGVDLAAI
                     NTDSQALAVNAAPIKLQIGRRLTEGLGTGANPEMGKFAANEDRAQIGELMKGADLVFL
                     TLGLGGGTGTGAGPVVAKIAKDNGAVVVGFVTQPFGFEGGIRRDVADRGLGELKKQLD
                     TLVVVSNEKILEIVPKNTSLEDAFARVDDILYQGVAALTDLITQPGLINVDFADLRSI
                     ILEGGEGILGTGSATGENRAEEAARLAISNPFLKREELVSARKILVNVSGPKELGIFE
                     MRRAVEVIDEALSPYQGERFCGAVIDPALNEEMKVTVIATGLERVGLTGMVTAEAVPA
                     DRPPTPPRPPRPNKRNEETDKREVTLFNTNGETGGGASPETANHDIPAYLRQKNSKGQ
                     P"
     gene            complement(7396..>8631)
                     /gene="ftsA"
                     /locus_tag="PKN80_03880"
     CDS             complement(7396..>8631)
                     /gene="ftsA"
                     /locus_tag="PKN80_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNQ35186.1"
                     /translation="AMMNNEDYLVGIDIGSEKISVLMVDVTSPRLPVLGFAAVPTRGM
                     RQGAVTSLGDLGAAIERAVLEVEEASGKAIEKVHLSLNGEHFRPVVRQGEVEIRNRQD
                     EVFESDRQAVLSRALPVPLSEGEVLHCIVQEFLLDDQRGIDNPVGMYGRNLKANIYLI
                     TAAVNNLKNIRRCLADFKLEAASVGYAPLLAAGAVLTPEELAEGVAYLDFGKTTTTLL
                     IFKSTLREVGVVHRGGDRLNFALRYCLNTTPAEAERLKRNYGSCLADGLDVQEEIMVS
                     NAQGEVRQTTSRGFIAEVINAELKDMFSPLQSFFIKNRPVIPAGLVLGGGGAQLPGLV
                     DFMEKDLKLPARVGMPDGFDGWEKVIKSPAYNSLLGVVKTNLPGTQGREEGGGRRSGV
                     TRVLTRHFRKITDILKDNF"
BASE COUNT         1524 a         2901 c         2698 g         1508 t
ORIGIN      
        1 cagttccgcc tccatttccg gcagaacggc ggtatccgaa taggcctcgt aaaccagttc
       61 gccggtatta agcagatcct ggaggcttga cgaccagcgg gcctcccgcc ctttgttctg
      121 gccgttgctc cggaccaccc gtcccggcat gaccaggacc gtgtgcacct tgacgccctg
      181 catcatggcc agcatgccca tgggaccggg accgccgccc ggttgtccgc cttcgcccat
      241 gcccatgcct ttcttcatct ggctcatgtc cagcaccatc cggaagtgat aaagcccttc
      301 cttgtttttc tcccagacga tctccttggc gcccaccggt atctccttcg acttgccggc
      361 cagattcagg atgtcgtcgt agtaaacgga gaaggtggtc cggatgtcgg aaagtccgct
      421 gatgcccatc ttttcaagct gctggaccgg gttctcgccg ccgggcaggc cgccgccccc
      481 gggctggccg cctccgaaac tgccggccag gccgaccagg gcaccggtca tgttgaccgg
      541 atccatggta atcagaacgg tctgcttgcc gttccgttcc gggtagatgt agtaccggtt
      601 ttcgatctcc atgcaaccgg aaagaaccac cgccaggccg accaggccga ataacatcgc
      661 ccaccgcttc atttccgcct cctgtttgaa gtgccacccc gtttgggctt tgttttgatt
      721 ggatgatccc cgtttgattc ccgtttaacc ggcccgggat tcagccggga cagctcggcc
      781 agcagcgcct cggccaggcc ggcagccggc ggccgttcca ccacccgccc ctttcgaaaa
      841 aggagtccgc gcttcccgcc gcaggccacg ccgatatcgg ccgcggccgc ttcgcccggt
      901 ccgttaacct cgcagcccat caccgccacc ttgaaaccgg cgtatcgctc cggatagccc
      961 ggcagccgtt tcagtccgtc ctccacccgg tcgaggatcg agaagaggtc ggaacggcag
     1021 cggccgcagg tgggacagga gatgagttcc ggctgaaaat tgcgcagctc cagcgcctgg
     1081 agtatccagc ggccagccgc cacctcctcc accgccgggc cggagagcga gacccgcagc
     1141 gtttctccga tgccgtcggc cagcagggcg ccgatgccga tcgaactttt gaccagggcc
     1201 ggaccgcccc ggccggcctc ggtgacgccc aggtgaagcg ggtaatcgaa gtgccggcgg
     1261 aagaggcggt tggccgccag ggtaaccggg accgaggatg atttgagcga aacgaccagg
     1321 ttgtggaaat cgagcgtctc gaaattttcg aggtaggcgg ctgccgttcg gaccatcaac
     1381 cgggccgttt ccaggccgga gaccggttct cccgggccgc cgggcagcga accggagttg
     1441 acgccgattc ttatggccgc gcccttccgc cgggccagcc ggatgatctc ggccagccgg
     1501 accggatcgt ccaggttgcc gggattgagc cggaccttgt cggctccggc ggccagggcg
     1561 cccagggcga ggtcgccccg gaaatgaatg tcggccacca ggggcaggcg ggagattgat
     1621 ttcaggcggg cgaaggcggc caggtcttct tcatccttga ccgccaggcg gaccagctcg
     1681 gcgccggcgg cggccagggc ttccagttcc agccgggtgg ctttcaggtc ggcggtacgg
     1741 gtcttggtca tcccctgaac ccgtaccggg gcgccgccgc cgacggtcaa ccggccgatc
     1801 cgcaccgggc gcgccaccgt actctttatg ctcatgcctg cgctccgatc gcgaatcacc
     1861 tggtcgcgcg gccagccggc ccttaccggc cgacaattat ccgcagcagg tcgttatagg
     1921 tgacgaaaac cgccagggcc agcaccaggc ccagcccgat catctggccg acctcgatga
     1981 agcgcgggct gggcttgcgc ccggaaatcc gctccagcat gaccccgaag aggtggccgc
     2041 cgtcaaaaac cgggagcgga aagaggttga ccaccgcgag gttgacgctc aggagggcca
     2101 ggaaatagag gaagttgctc cagccgagcc ggaaaaaacg ggtactcaac tgggcgatgc
     2161 ccagcggacc ggccaggtcg cggccggaca tcttccgggt gaagatccgg cccaggtaat
     2221 agaagatgtc gtgaacgacg ccggcggtgg ccttgccggc ccgtcccagc gccgcgaaca
     2281 gaccgtattt ctcacgctgg atctgggggc cgatgccgac caggccgatc cgccgcgaac
     2341 cctggccggc ctcgagagtt tcggcccgcg gcgtaacccg gacctggaag acccgcccgg
     2401 cccggtccac ctccaggctg atctcccggt ccggataacg atggatctcg ccggagagcg
     2461 aaggccagtc cttgaccggc accccggcca cccggatgat ccggtcgccg gcctggaggc
     2521 cggcctggac ggccgggtaa cccggctcca gcccgccgat caccggttcc cggtagttgg
     2581 ccagttccac accgagcagg aagaccagca cgaaggccag gaatcccagc agtaaattgt
     2641 gcaccgagcc cattaccagg atcagcatcc gcttgccggc cggctgggcg aaatactcgt
     2701 cccccttcgg cgtcgcgcgg ccgtaattct cctccccggc cagtttcacg gcacccccga
     2761 acggaatcag cgaaacctgg tactgggtgc cgccgcgctg aaaaccgaag agcttcggac
     2821 caaagccgac cgtaaagagc tcgacgcgca ccccggcgcg gcgcgccgcc cagaagtgtc
     2881 ccagttcgtg gctgatgatc agaatcccga agaccagcac gaccgaaaac aggttcacca
     2941 gaaaagaaag tatggcgttc atcaatactc cctattttat cacaatgtac ccgcgcgcgg
     3001 gactctcaat cttcccggcc ggccttgaaa tcggcgatga tctgcttaag ggcggtctcg
     3061 agcgaaaccc tcggccggta accgatggtc gcctccagcc gggtcaggtc cggcacccgg
     3121 cgccgcatgt cttcaaagcc ctccttgtag tcctgctccg gatccaggtg taccagcggc
     3181 gaccggcttt tgcaaagcgt gcggatccgc cgggccaggt cctcgatcgt cacctcttcc
     3241 cggctgccga tgttgaagac cccgccgacc gcggccgggg actcgaccag acggcccagc
     3301 gcctcgatca cgtccgagac gcaggtaaag cagcggctct ggcgcccgtc cccgtaaatg
     3361 gtgatcggcc gtccctcgag cgcctgccgg acaaaacggg ggaccaccat gccgtaacgt
     3421 ccggtctggc gcgggccgat cgtgttgaag aggcggacga tgatgaccgg cagtccggcc
     3481 tcggcggcat aggccagggc gaagaactcg tccagggcct tgctgcaggc ataggcccag
     3541 cggcggatgg tggtggcgcc gagcacgatg tcgttctcct cggcgaacgg cacgttttcg
     3601 ttcttgccgt acacctcgct ggttgaggcc agcacgatct tgcgccgggc ggccgcgcag
     3661 gcctccagca ggttgcaggt cccctcgacg ttggtgcgca acgattgcag cggctgctcg
     3721 atcaccgtcc ggactcccac cgcggcggcc aggtgaaaga ccgcgtcggc ctcgccaacc
     3781 agccgttcca ggaccggccg gttcaagatc gaatcaacca ggatctgcag gttgggatga
     3841 tgcttgacag cctccaggtt cttgaggcgg ccggtgctca gatcgtcgag caccgtcacc
     3901 cgatgcccgg ccggcagcag gtgctcggtc aggtggctgc cgatgaagcc ggcgccgccg
     3961 gtaataaggt atcgcatggt ttaagtgccc tcctgccagg aggaaaggta cttctcctgg
     4021 tccggggtga gccgatcgat ttcgacaccg ctggcggcca gtttcaggcg ggccacctcg
     4081 cggtcgatct cctccggaac cgggtagagc cccggcttga tccggccccg gttcttcacc
     4141 aggtgctcgg ccgaaagggc ctggttggcg aaactcatgt ccatgaccgc cgacgggtgc
     4201 ccctcggccg cggccaggtt gagcaggcgg ccctgggcca gcaggtaaag gcggcggccg
     4261 tcggaaagct cgaactcctc gacctggtcg cggatcagcc ggcgggcccg gctgagcccg
     4321 gccagggccg ggatgtcgat ttcgacgtcg aagtggccgc tgttggccag gatggcgccg
     4381 cttttcatga ccctgaagtg ttccgcccgg aagatggacc ggttgccggt ggcggtgatg
     4441 aagacatcgc cgaaccgcgc cgcctcaagc gaaggcatga ccaggaaccc gtccatgagc
     4501 gcctccagcg cccggaccgg ttcgatctcg gtgacggcca cgatcgctcc caggccgcgg
     4561 gcgcgctggg cgatgccgcg gctgcaccag ccgtagccgg ccaccaccac cttccgtccg
     4621 gccagcagca ggttggtcgc ccgcagcagt ccgtcgatgg tcgactggcc ggtcccgtag
     4681 cgattgtcaa acaggtgctt ggtgtccgcc tcgttcaccg ccacgatcgg gaaacgaagc
     4741 cggccttcac gggccaggtt gcgaaagcgg atgaccccgg tggtggtctc ctcggtaccg
     4801 ccgaagacct cccggcagaa agcctcgtcc cggcggcgcg cctggctgat gagatcggcc
     4861 ccgtcatcca gcagcacgga gggccgggcc gacaggaccg cctccaggtg ccggtaatag
     4921 gcgtcccggt cctcaccccg gaccgcgtgg acgtgtatcc cgtatttcac ggccagggcg
     4981 gcggccaccg cgtcgttggt gctcagcggg ttggaggcgc acagaaaaac ctcggcgccg
     5041 ccggcctgga gcgccagggc caggttggcc gtctccgagg tgacgtgcag gcagccgccc
     5101 aaacgcaggc cggcgaaggg gtgtgactgc gcgaaacgct cccgtatcga aagcagcacc
     5161 ggcatctccc ggcccgccca ttctacgcgg ttgacaccct cgtcgaccaa tccccgatcc
     5221 ctgatctcaa actcggccat aagcctcctt cgcatcgcac cggaaagcaa ccgtcctccg
     5281 gaaggggacc ggagaccctg gaaccggtgc tcttgacaag attaaaattt tatgttctac
     5341 tatgtaaaaa caatcgatac ccggtttcgg aaccagatct tgaaaacaga cgaagcggaa
     5401 cgacaactct gaaacgatgt cgcgacaacg gtttgcgact taataaagtt actcgaaagg
     5461 gggtgaaaat ttatggcgac taagaaaaag cccgtcaaga aggcggcggc taagaagccc
     5521 gcgaagaaga ccgcgaagaa agccactaag aagggttgct gcaagtaagc aagcaacggt
     5581 cgtaaaggaa acgggagata tcttctttcg aacggtttga caggtcgtaa acttcgccgc
     5641 ggcgagacaa cagaaaagat tgatgtctcc ctttttcttt tccgaagaag cccggctcgt
     5701 tcaccagtat aaaatccagg ttcttccgcc tcagcttcgc ccgccccgcc tccaccaggt
     5761 tgttggtctc cagcgcgcac ccgatcagcg tctgtccatt cttcttcaac cgtccgcagc
     5821 cggccagtat gtccgggttc ggaaagaggg tgagcgaaaa cttgcgcttg catttcagct
     5881 tggtgcgccg aaagcgcggc cgccagtcgc ccacggcggc cgtcatgaag agcgcctggc
     5941 aatccgggaa gtggcggcgc agggcggttt ccatctggcg cgccgtttcc accctgacca
     6001 ccttgacgcc ggccgggttc tccagcccgg tcggaccgca gaccagcacc gcctcgtgtc
     6061 cgcgccggac ggcctcctcg gccagcaggt aaccaagccg gccggtggcc gggttgctga
     6121 tataacggac cgggtcgatc cactcgcggg tggggccggc gctgatcagt atcttcatgg
     6181 ctggcccttg gaattcttct gccttaaata agccggtatg tcatgattgg cggtctcagg
     6241 cgaggcgccg cccccggttt caccgttggt gttaaagagc gtgacctccc gcttgtctgt
     6301 ttcctcattt cttttgttgg gccggggcgg gcgcggcggc gtgggtggcc ggtcagccgg
     6361 gaccgcttcc gccgtaacca ttccggtcaa gcccacccgt tccaggccgg tggcgatgac
     6421 ggtcaccttc atctcctcgt taagggccgg gtcgatgacg gccccgcaaa aacgctcgcc
     6481 ctggtaaggc gaaagcgcct cgtcgatcac ctcgaccgcc cggcgcattt cgaaaatgcc
     6541 caattccttg ggaccggaga cgttgaccag gatcttgcgg gcgctgacca gttcctcgcg
     6601 tttcaggaag gggttgctga tggccagccg ggcggcttct tccgcccggt tctcgccggt
     6661 ggccgaaccg gtgcccagga tgccctcgcc cccctcgaga atgatggagc gcaggtcggc
     6721 gaaatcaacg ttgataaggc cgggctgggt gatcaggtcg gtcagggcgg ccaccccctg
     6781 gtagaggatg tcgtccaccc gggcgaaggc gtcctccagc gaggtattct tcggcacgat
     6841 ctcgagaatc ttctcgttgg agacgaccac cagcgtgtcc agctgttttt tcaactcgcc
     6901 caggccccgg tcggccacat cccgccggat gccgccctcg aaaccgaagg gttgggtcac
     6961 gaagccgacg acgaccgcgc cgttgtcctt ggcgatcttg gccaccaccg ggccggcgcc
     7021 ggtgccggtg ccgccgccca ggcccagggt caggaagacc aggtcggccc ctttcatcag
     7081 ctccccgatc tgggcccggt cctcgttggc cgcgaacttg cccatctcgg gattggcgcc
     7141 ggtgccgagc ccctcggtca gccgccgtcc gatctggagc ttgatcggcg ccgcgttgac
     7201 cgccagcgcc tggctgtcgg tgttgatggc cgccaggtcg acgccgctca agcgttccac
     7261 catccggctg accacgttgc cgccggcgcc gccgacgccc accaccttga tcttaaccgc
     7321 gcccatgttc tgctcttccg gtctgatcct catcatcata atccgccgcc cccgccgacc
     7381 aaccgcgtcg accgcctaaa aattatcctt cagaatgtcc gtgatttttc tgaaatgcct
     7441 ggtcaatacg cgcgtcaccc cgctccggcg gccgccgccc tcctcccggc cctgggtgcc
     7501 cggcaggttg gtcttgacca cgcccaggag cgagttgtag gccgggctct tgatcacctt
     7561 ctcccagccg tcgaaaccat ccggcatgcc cacccgggcc ggcagcttca gatccttttc
     7621 catgaagtcg accagtccgg gcagttgcgc gccgccgccg cccaggacca ggccggcggg
     7681 aatgaccgga cggttcttga tgaagaagga ttgcaggggg ctgaacatgt ccttgagttc
     7741 ggcgttgatg acctcggcta tgaaaccccg gctggtggtc tggcgaacct cgccctgggc
     7801 gttggaaacc atgatttcct cctgaacgtc caggccgtcc gcgaggcagg agccgtaatt
     7861 cctcttgaga cgctcggcct cggcgggggt ggtgttcagg caatagcgca gcgcgaaatt
     7921 gagccggtcg ccgccccggt ggaccacgcc cacctcccgc agggtggatt taaaaatcag
     7981 gagggtggtg gtcgtcttgc cgaagtcaag gtaggcgacg ccctcggcaa gctcctcggg
     8041 ggtcagaacc gccccggcgg ccagcagcgg cgcgtagccg acgctggccg cttccagctt
     8101 gaaatcggcg aggcagcgcc tgatgttctt gagattgttg accgcggccg tgatcaggta
     8161 gatgttggcc ttgaggttcc ggccgtacat gccgaccggg ttgtcgatgc cgcgctggtc
     8221 gtccagcagg aactcctgga cgatgcagtg caggacttcg ccctccgaaa gcggaaccgg
     8281 cagggcgcgg ctcagaacgg cctggcggtc gctctcgaac acctcgtcct ggcggttgcg
     8341 gatctcgacc tcgccctggc gcacgaccgg gcggaaatgc tccccgttca ggctgaggtg
     8401 aaccttctcg atggcctttc ccgaggcctc ctctacttca agcaccgcgc gctcgatggc
     8461 cgcccccagg tcgccgaggc tggtaaccgc accctgcctc atgccccggg tcggaaccgc
     8521 ggcgaaaccg aggaccggca gccgcggcga ggtgacatcg accatcagga ccgaaatctt
     8581 ctcggaaccg atgtcaatac cgaccaggta atcttcgtta ttcatcatgg c
//
LOCUS       DAOQYN010000067         8621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8669_length_8621_cov_4.080901, whole genome shotgun sequence.
ACCESSION   DAOQYN010000067 DAOQYN010000000
VERSION     DAOQYN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8669_length_8621_cov_4.080901"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PKN80_03885"
     CDS             <1..769
                     /locus_tag="PKN80_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ35187.1"
                     /translation="TVLHITPSYLLYLADYLARAGLDPARDLKLKRAYVGAEPYSEET
                     RRKIESLYGIDIYNSYGLSEMNGPGVAFECAGKQGMHLWEDNFLMEVVDPETGRPRAE
                     GEAGELILTTLNREAMPIIRYRTRDITSVISEPCACGRTHRRITRIAGRADDMFIVRG
                     VNIFPQQVERVLMAVSGVAQNYQIILDSYDQMTVRVEIQREVFDGHVEHLIRLKNLII
                     DRLRSEIVVRPEVELVEPGSLPTFEGKGKRVIDRRTM"
     gene            814..1245
                     /locus_tag="PKN80_03890"
     CDS             814..1245
                     /locus_tag="PKN80_03890"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNQ35188.1"
                     /translation="MRQISVFASNRPGKIAAITGVLAEAGINIMGMNIASSGDFGVIK
                     FLVNDLEKADRALREKGFTISLNEVLAIEMEDVPGGLHRIADLLYRKNINVEYSYGLP
                     MKPHRNAILIIEVEDVEQAKLLLKNEPLRFLSEPEIQKERG"
     gene            1245..2543
                     /locus_tag="PKN80_03895"
     CDS             1245..2543
                     /locus_tag="PKN80_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNQ35189.1"
                     /translation="MIWNPAAEAVPLKERQSLQLERLRRIVDLAYHRVPFYRKSLDRI
                     KLKPGHIKTLKDIEKLPFTTKDDLREGYPFGMFAVKLEEIVEFHTSSGTTGKPIVTGY
                     TRGDIELWSEVMARALTMAGAGPQSIIQNAYGYGLFTGGLGTHYGARKIGAALVPISA
                     GNTARQLQVMQDFGTTLLACTPSYSLYLAETAEEQAGGLRRLKLKSGCFGAEMWTERM
                     RDEIERRLHLKALNIYGLTEVIGPGVAQECLRQSGLHIAEDHFFPEIISPETLRTCPE
                     GESGELVLTTLTREGTPILRFRTKDITSLHREDCKCGRNSIKMARITGRSDDMLKVRG
                     VLFFPSQIEKALLEIKGVEPHYQIILTRPHQLDEIEIQVETTRHIFSDEIKRLEELRS
                     QIEKHLEHAINLRVKVTLVEPKKLPRSEGKAKRIVDNRQM"
     gene            2661..3092
                     /locus_tag="PKN80_03900"
     CDS             2661..3092
                     /locus_tag="PKN80_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HNQ35190.1"
                     /translation="MKVKQVSVFLENKRGRLLEALRGLEADRINIRALSLADTLEFGV
                     LRLIVNNPDQAKKTLEKKGFTVGETEVIAVGVDDRPGGLASVLEVFQQAAIDVEYLYA
                     FVEKKKSQAVVVFRIEKTASGIKALKAAGVKVLTAEEIYRL"
     gene            complement(3089..4681)
                     /locus_tag="PKN80_03905"
     CDS             complement(3089..4681)
                     /locus_tag="PKN80_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ35191.1"
                     /translation="MKKELLSGNEAIARGAYEAGIAMAVSYPGTPATEILEYLAAGFP
                     VEAQWTTNEKVALETAIGVSYTGRRALCSMKHVGLNVAADPLMTAAYLGIRGGLVLVV
                     ADDPGAYSSQNEQDSRFYARFARIPCLEPADAAQAHLMVREAFELSEKISRPVMLRSV
                     TRLSHGRSPVEIGAPRSPAPPDLKKDPARMIAVPSHVLAAHRDLLKQQPAIIEWGKGS
                     GFNRIEKSAGQRRGVIACGVGAAYARETLGEDCALLQVGFYPLEPGLIKEFGEGLEEI
                     WVVEEGEPLVEEMARCSFRNVRGKLSGDLPPVGELGPEPLAAIQKKEKPLSTPATSSP
                     LPSRPPVMCAGCPHRELYLALKAAGPSFTAGDIGCYTLGASPPLSALDTCLCMGAGIS
                     QAAGIAHQGIRRVAAVIGDSTFIHSGIPALIGSVYNRANLLVLILDNASVAMTGHQPT
                     PQTGIRADGRPGGQVDLEAICRACGVDSVTVLDPLRKEEMTRLLKEKLEADGVHVVIS
                     RRPCVFVGRRAVRPENGGGNKT"
     gene            4814..5506
                     /locus_tag="PKN80_03910"
     CDS             4814..5506
                     /locus_tag="PKN80_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNQ35192.1"
                     /translation="MANNGNRGRRRAVIFDIDGVIFDTERIHLQAWANLFERIGVQLG
                     PADYRRGVGVADQLFLAELKRNGRVPRRPSIEALLNSKEKEVERLVAAGGVRVFPGVP
                     ELLTRLDGRADVCGASNSTRSYIRAFFSGHPELQARFRFILTRDDIRHPKPAPDIYLA
                     CSRRIGIAPADCLVIEDSPVGIEAARRAGMRCLAVTNSLPAARLGGAGRIVKRLGYRT
                     VIDLMNIDGGEA"
     gene            5503..6066
                     /locus_tag="PKN80_03915"
     CDS             5503..6066
                     /locus_tag="PKN80_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ35193.1"
                     /translation="MKILLVIGSRNHEGQTARAAQALAAGAVGAGAAVEMVFLTDLKL
                     SACRQCEANGWGLCRAEGRCVIEDDFAGLVEKMRGAAALVVATPVYFSDLSESLKLFL
                     DRLRRTNRHENGRAGLAGKPALGICVAGGGGGGAPSCAFNLDRILQTCGFEAVDLVPV
                     RRQNLELKLALLRLTGEWLARSADGGN"
     gene            complement(6063..6776)
                     /locus_tag="PKN80_03920"
     CDS             complement(6063..6776)
                     /locus_tag="PKN80_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNQ35194.1"
                     /translation="MLAYILLGGLGTRLRPLTYLTPKPLLPLAGKPFLWYQLNLLARH
                     GVRRVILGTGYKEENFRAVSDSFAIPGLEVEFVVEREPRGTAGALALARKSLSKTAFV
                     LNGDILTDLDLSAMLKEHRERRSLVTLALYQVAYPSRYGLVDFDEAGRVSAFREKPRE
                     IPRRAWINAGVYILEPEAVRLIPEEGNCSLEREVFPALIKSDRPVYAYRHTGYWLDIG
                     TLHAYCQAKGDLKSGRLVP"
     gene            complement(6879..7823)
                     /locus_tag="PKN80_03925"
     CDS             complement(6879..7823)
                     /locus_tag="PKN80_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNQ35195.1"
                     /translation="MAPRKVEWELMLKHELDQAVRDCPVAYLSFGLCEPHGFYNPLGL
                     DGLKAHGLALEAARAGGGVAAPASFWHIHEDNSRSRAFLGRITELPLYLTSIPGPLFF
                     QNFIYQLRACANAGFQAVVAITGHYGGVEYWLKWYAKLFQKHLAPLPVWALADWEVID
                     HQDYKGSHAGICETAQLLALFPGLPDLKHPGLKEEDPYVGGRLTPVREKVNPELGRAI
                     VASQVRNLARGAEEMLARRPGPSENIFLTMETAIKAWPVIEKEAPAWPGGLDETAAAD
                     LFRERVEPYWQKARRSNGRLPGHVFPAAPPESIDGPEW"
     gene            complement(7848..7919)
                     /locus_tag="PKN80_03930"
     tRNA            complement(7848..7919)
                     /locus_tag="PKN80_03930"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7885..7887),aa:Thr,seq:cgt)
     gene            8037..>8621
                     /locus_tag="PKN80_03935"
     CDS             8037..>8621
                     /locus_tag="PKN80_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35196.1"
                     /translation="MTDLERFYACLEYRPVDRRPFWTWGAWPETMQRWEREGHAAELE
                     ELHRRYGDRKLKYGGWFFPCPPFEKKAVSEDAEHLVYVNEEGITMRELKKNPRSSMPQ
                     FISFPVRTRDDFRRFWRERMNPDLAPRLGSDWREKLRAIRARREPFILFSDRWGGFFG
                     PLRNLLGVERLCTLFYDDPAFLEEMMEADADFILA"
BASE COUNT         1668 a         2714 c         2694 g         1545 t
ORIGIN      
        1 cacggtcctt cacatcactc ccagctacct gctctacctg gccgattacc tggctcgggc
       61 cggtctcgat cccgcgcgcg acctgaaact gaagcgggcc tacgtcgggg cggaacctta
      121 cagcgaggaa acgcgccgga agatcgagag cctctacgga atcgacattt acaattccta
      181 cggtctttcg gagatgaacg gtcccggagt ggccttcgaa tgcgccggca agcagggcat
      241 gcacctctgg gaggataact tcctgatgga ggtcgtcgat ccggagaccg gccgtccccg
      301 ggccgagggg gaagcgggcg aactgatcct gaccaccctc aaccgcgagg cgatgccgat
      361 catccgctac cggacccggg acatcacctc ggtcatctcc gagccctgcg cctgcggccg
      421 cacccatcgt cggatcaccc gcatcgccgg ccgggccgac gacatgttca tcgtgcgcgg
      481 ggtcaacatc tttccccagc aggtggaacg agtcctgatg gctgtctccg gcgtggccca
      541 gaattaccag atcattctcg attcgtacga tcagatgacc gttcgggttg aaatccagcg
      601 cgaggtcttt gacggtcacg tcgaacatct gataagatta aagaatctga tcatcgatcg
      661 cctccggtcc gagatcgtgg tgcgcccgga agtcgaactg gtggaaccgg gttccctgcc
      721 gaccttcgag ggcaagggaa aacgggtgat tgaccgccgg acgatgtaaa gatccggccg
      781 gggccggccg gaaagggagg gattatgacg ggagtgcgcc agatttctgt cttcgcctcg
      841 aatcggccgg gcaaaatcgc cgccatcacc ggcgtgctgg ccgaggccgg catcaatatc
      901 atgggtatga atatcgccag cagcggcgat ttcggggtga tcaagttcct ggtcaacgac
      961 ctggagaagg ccgaccgggc cctccgggag aaaggattca ccatttccct gaacgaggtg
     1021 ctggcgatcg aaatggagga cgttcccggc ggcctccacc gcatcgccga ccttctctac
     1081 cgcaaaaaca tcaatgtcga gtattcctat ggcctgccca tgaaaccgca tcgaaacgcc
     1141 atactgatca tcgaggttga ggacgttgaa caggccaagt tgctgctcaa aaatgaaccg
     1201 ctccgttttc tcagcgaacc cgaaatccaa aaggagagag gctgatgatc tggaatcccg
     1261 ccgccgaggc agtgccgctg aaggaacgcc agtcgctcca gctggaacga ctccggcgga
     1321 tcgtcgacct ggcctatcac cgggtgccgt tttaccgcaa gtccttggac cggatcaagc
     1381 ttaagccggg gcacatcaag acgctcaagg atattgaaaa acttcctttc accaccaagg
     1441 acgacctgcg ggaaggttac ccgttcggga tgtttgcggt caagttggag gagatcgtcg
     1501 aatttcacac ctcttccggg actaccggca aaccgatcgt caccgggtac acccgcggag
     1561 acatcgaact ctggagcgaa gtgatggccc gcgccctgac catggccggg gccggaccgc
     1621 agtctatcat ccagaacgct tacggttacg ggctcttcac cggcggcctg ggcacccatt
     1681 acggcgcccg gaagatcggc gcggcgctgg tgccgatttc ggccggcaat accgcccgcc
     1741 agctgcaggt gatgcaggat ttcggcacca ccctgctggc ctgcacaccc tcctactccc
     1801 tgtacctggc cgaaacggcc gaggaacagg cgggcggcct ccgcaggttg aagctgaagt
     1861 ccggctgttt cggcgccgaa atgtggaccg aacggatgcg cgatgagatc gaacggcgcc
     1921 tgcacctcaa ggcgctcaac atctacgggc tcaccgaagt catcggtccc ggggtggccc
     1981 aggaatgcct gcgccaatca ggcctgcaca ttgccgaaga ccactttttc cccgagatta
     2041 tctccccgga aacgcttcgg acctgtccgg aaggcgaatc cggcgaactg gtcctgacca
     2101 cgcttacccg cgaggggacc ccgatactgc gtttccgaac caaggacatc accagcctgc
     2161 accgggagga ctgcaagtgc ggccggaaca gcatcaagat ggcccggatc accggccgca
     2221 gcgacgacat gctcaaggtg cgcggggtcc tcttcttccc ctcccagatc gaaaaggccc
     2281 ttctggagat caagggggtg gaaccgcact accagattat tctaacccgg ccgcaccagc
     2341 tggacgagat cgagatccag gtggagacga cccggcatat tttctccgac gaaatcaagc
     2401 ggctggagga gttgcgctcc cagatcgaaa agcacctgga gcatgccatc aacctgcggg
     2461 tcaaggtcac cctggtcgag cccaaaaaac ttccccgcag cgaaggcaag gccaagcgga
     2521 tcgtcgataa ccggcagatg tgaggccggc cgccgccgga agcactgatt tttgacttcg
     2581 gagaagaagc gccggggggc gggagattcc ggccgccatt ttttcgaggg aaaccgacac
     2641 gcgagcaaag ggggcgaaac atgaaggtca agcaggtttc ggtctttctg gagaacaaac
     2701 ggggccggtt gctggaggcg ctccgggggc tggaggcgga ccggatcaac atccgggccc
     2761 tctcgctggc cgataccctg gagttcggcg tgctgcgcct gatcgtcaac aacccggacc
     2821 aggccaagaa aacacttgag aagaaaggat ttaccgtggg ggaaaccgag gtgatcgccg
     2881 tcggcgtgga tgatcgtccc ggcgggctgg cctcggtcct ggaggttttt cagcaggcgg
     2941 ccatcgacgt cgagtacctc tacgccttcg tcgagaagaa gaagagccag gcggtggtgg
     3001 tcttccgcat tgaaaaaacc gcctccggca tcaaggcgct caaggcggcc ggcgtgaagg
     3061 tgctcaccgc cgaggaaatc taccgccttt aggttttgtt ccccccgccg ttttcaggac
     3121 ggaccgcccg gcggccaacg aagacgcacg gccgccggga aatgaccacg tgaaccccgt
     3181 cggcttccag tttttccttc agcaggcggg tcatttcttc cttccgcaaa ggatccagca
     3241 ccgtcaccga atccaccccg caggcccggc agattgcctc gaggtcgacc tgtccgcccg
     3301 gccggccgtc ggcccggatt ccggtctggg gcgtcggctg gtggccggtc atggccaccg
     3361 aagcgttgtc gagaatcagg accagcagat tggcccggtt gtaaaccgaa ccgatcaagg
     3421 ccggaatgcc ggagtgaata aaagtggaat cgccgattac cgccgccacc cgccggatgc
     3481 cctggtgggc gatgccggcc gcctggctga tgcccgcacc catgcaaagg caggtgtcca
     3541 gcgccgagag cggcggactg gcgcccaggg tgtagcaacc gatgtcgccg gccgtgaatg
     3601 aaggtccggc cgctttcaaa gccagataga gttcccggtg cggacagccg gcgcacatga
     3661 ccggcggccg ggagggcagc ggcgaagaag tggctggcgt ggaaagcggc ttttccttct
     3721 tctgaatggc ggccagtggt tcgggtccca gctcgccgac cggcggcaga tcgccggaaa
     3781 gcttgcctcg gacattgcgg aaggagcaac gcgccatctc ctccaccagc ggttccccct
     3841 cctccaccac ccagatttct tccagcccct ctccaaattc cttgattaag cccggctcga
     3901 gcggataaaa accaacctgg agcagcgcgc aatcctctcc cagcgtctcc cgggcgtagg
     3961 cagcgcccac tccgcaggcg atgacgcccc gcctttgccc ggccgacttc tcgatccggt
     4021 tgaacccgga gcctttcccc cactcgatga ttgcgggctg ctgcttcagc agatcccggt
     4081 gcgccgccag cacgtgactg gggaccgcga tcatccgggc cgggtctttt ttcaaatcgg
     4141 ggggcgccgg agaacgcggc gctccgattt caaccgggga ccggccatgg gaaagccggg
     4201 tgaccgaacg aagcatcacg ggccggctga ttttttcgga taattcgaag gcttcccgga
     4261 ccatcaggtg ggcctgggcg gcgtcagccg gctccaggca gggaatccgg gcgaaacggg
     4321 cgtagaaacg ggagtcttgt tcgttctggg aagagtaggc acccggatcg tcggccacga
     4381 ccaggaccag accgccccgg atgccgagat aggcggcggt cattaacggg tcggcggcca
     4441 cgttgagtcc gacatgcttc atggagcaga gtgcccgccg gccggtgtaa gagactccga
     4501 tggccgtctc cagagcgact ttttcgttgg ttgtccactg ggcttcgacc gggaagccgg
     4561 cggccaggta ctcgagaatt tcggtggccg gggtacccgg ataactgacc gccatggcga
     4621 tgccggcctc gtaagccccc cgggcgatcg cttcgttgcc ggaaagaagt tctttcttca
     4681 tcgacgcgca tccttttttt gaagaaaccg ttgaatttta acacatcagg ggccggattc
     4741 acaaccggcc ggctttggcg gccgctcccg gaaagtgata aaatctatct ccaatttcaa
     4801 cggggcggaa tcgatggcaa acaacggaaa ccggggccgc cggcgggcgg tgatcttcga
     4861 tatcgatggc gtgatcttcg ataccgagcg gatccatctc caggcctggg ccaacctttt
     4921 cgaaaggatc ggcgtgcaac tgggtccggc cgattaccgg cgcggggtcg gcgtcgccga
     4981 ccagctcttc ctggccgaac tcaagcggaa cggccgggtc ccgcgccggc catcgatcga
     5041 agccctgctg aattccaagg aaaaagaggt ggaacgcctg gtggccgcgg gcggtgtccg
     5101 ggttttcccg ggcgtcccgg aactgctgac ccggctggac ggccgggcgg acgtctgcgg
     5161 cgcctccaat tcaacccgtt cctacatccg ggccttcttc tccggccatc cggaattgca
     5221 ggcccgtttc cgattcatac ttacccggga cgacatccgc catccgaaac cggcccccga
     5281 catctacctg gcctgctccc gccgcatcgg aatagctccg gccgactgcc tggtcattga
     5341 ggattcgccg gtgggaatcg aggcggcgcg ccgggcgggc atgagatgcc tggccgtgac
     5401 caattccctg ccggccgccc ggctgggcgg cgccggccgc attgtgaagc gcctcggtta
     5461 ccggacggtc attgatctca tgaacatcga cggaggtgag gcatgaagat actgctggta
     5521 attggaagcc gtaatcacga ggggcagacg gcccgggcgg cccaggccct ggccgccggc
     5581 gccgtcggcg ccggggcggc ggtcgaaatg gtctttctta ccgacctgaa gctgtccgcc
     5641 tgccgccagt gcgaagccaa cggctgggga ctctgccgcg ccgagggacg ctgcgtgatc
     5701 gaagacgatt ttgccgggct ggtcgagaag atgcggggcg ccgcggcctt ggtggtggcc
     5761 acaccggtct atttcagcga cctgagcgaa tcgctcaaac tttttctcga ccggctgcgg
     5821 cgcacgaacc gccatgaaaa tggccgggcg ggcctggccg gcaaaccggc cctgggcatt
     5881 tgcgtggccg gcggcggcgg cgggggcgca ccctcctgcg ccttcaacct ggaccggatt
     5941 cttcaaacct gcggtttcga ggcggtcgac ctggttccgg tgcggcggca gaaccttgaa
     6001 ctgaagcttg cgttactgcg cctgaccgga gagtggctgg cccggtcggc cgacggcggg
     6061 aactaaggga caagacggcc ggacttcagg tcgcccttgg cctggcagta agcgtgcagg
     6121 gtgccgatgt ccagccagta gccggtgtgg cggtaagcgt aaaccggccg gtcggacttg
     6181 atgagggccg gaaaaacctc ccgttccagc gagcaattgc cctcttctgg tatgagccgg
     6241 accgcctccg gttccaggat atagaccccg gcgttgatcc aggcccgccg ggggatctct
     6301 ctcggcttct cccggaaggc gctcacccgg ccggcctcgt cgaaatcgac cagtccgtaa
     6361 cggctcgggt aggcaacctg gtaaagggcc agcgtaacca ggctccgccg ttcccggtgt
     6421 tctttcagca tcgccgaaag gtccaggtcg gtcaggatgt cgccgttgag cacgaaggcc
     6481 gttttcgaga ggctttttcg ggccagggcc agagcgccgg cggttcccct cggctccctc
     6541 tccaccacga actccacctc cagtcccggg atcgcaaagg aatccgagac ggcccggaaa
     6601 ttttcctcct tgtagccggt gcccaggatg acgcgccgta cgccgtggcg ggccaggagg
     6661 ttcagctggt accagaggaa gggttttccg gccaggggca ggagcggttt cggggtaagg
     6721 taggtgagcg gccgcagccg ggtacccagt ccgccaagca ggatataggc aagcatatca
     6781 agaatcaacc ctcaccagga gtaaggcctt ttccgcaacc ggttccgccg ccgggaaaaa
     6841 accaagcctg tccgttagct tcttccatcc gtgccggttt accattccgg gccatcgatg
     6901 ctctccggcg gcgcggctgg aaaaacatgt ccgggcagac ggccgttgga acgcctggcc
     6961 ttctgccagt atggctctac gcgctcccgg aataaatccg ccgccgccgt ttcatccagt
     7021 cctcccggcc aggccggagc ctccttttca atcaccggcc aggccttgat cgccgtctcc
     7081 atcgtcagga aaatgttttc ggacggtccc ggccgccgcg ccagcatctc ttccgctccc
     7141 cgcgccaggt tccgcacctg gctggccacg atggcccggc ccagttccgg gttgaccttc
     7201 tctcggaccg gagtcagccg gccgccgaca tagggatcct cctccttgag tccgggatgc
     7261 ttcaggtcgg gcaggccggg aaagagtgcc agcaactggg cggtttcaca gataccggcg
     7321 tggctgccct tgtagtcctg gtgatcgatt acctcccagt cggccagggc ccagaccggc
     7381 agaggcgcca ggtgtttctg aaagagtttg gcgtaccatt tcaaccagta ctcaaccccg
     7441 ccgtaatggc cggtgatggc caccaccgcc tggaaaccgg cattggcgca ggccctgagc
     7501 tggtagatga agttctgaaa gaagagcggt ccgggaatcg aggtcaggta gaggggcaac
     7561 tcggttatcc gtccgaggaa agcccggctg cgggaattgt cttcgtggat gtgccagaag
     7621 gaggccggcg ccgccacgcc gccgccggcg cgggcggctt caagcgccaa tccatgcgcc
     7681 ttgaggccgt ccagaccgag tgggttgtaa aatccgtgcg gttcgcaaag gccgaaactc
     7741 aggtaggcaa ccgggcaatc ccggaccgcc tggtccagct catgcttgag catcagttcc
     7801 cactcgactt tgcgcggggc catcgcggtc ctcccaaaaa aaaccggagc cacctggggg
     7861 atttgaaccc tcgacctgag cattacgaat gccctgctct accgctgagc taaggtggct
     7921 ttatctattt cagaggaatt gaaattttac ccgaaaatgc tttccctgtc aaagtttcct
     7981 ggccggccaa cacccccgtt gtgataaaat atcaggaatt cgaacaaggg ggtccgatga
     8041 cagacctgga aaggttttac gcctgtctcg agtaccggcc ggtggaccgc cgcccgttct
     8101 ggacctgggg cgcctggccg gagaccatgc agcggtggga acgcgaggga catgccgccg
     8161 aactggagga gctccaccgc cgatacggtg accgcaagct caaatacggc ggctggtttt
     8221 tcccctgtcc gccctttgaa aaaaaggcgg tctccgagga cgcggaacac cttgtctacg
     8281 tgaacgagga aggcatcacg atgcgggagt tgaagaagaa tccccgcagc tcgatgcccc
     8341 agttcatatc cttcccggtc cggacccgcg acgatttccg ccgtttctgg cgggaacgga
     8401 tgaaccccga tctcgccccc cggctgggtt ccgactggcg ggagaaactc cgggccatcc
     8461 gggcccggcg ggaacccttt attctcttct ccgaccgctg gggcggcttc ttcgggccgc
     8521 tccgcaacct gctgggcgtg gagcgactct gcaccctttt ctatgacgac cccgccttcc
     8581 tggaagagat gatggaggcg gatgccgatt ttatcctcgc c
//
LOCUS       DAOQYN010000068         8591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8706_length_8591_cov_4.142338, whole genome shotgun sequence.
ACCESSION   DAOQYN010000068 DAOQYN010000000
VERSION     DAOQYN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8706_length_8591_cov_4.142338"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..617)
                     /locus_tag="PKN80_03940"
     CDS             complement(84..617)
                     /locus_tag="PKN80_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ35197.1"
                     /translation="MISDVILDSIIVLSLVTTFLGPLVSKRLLYPDSKRLGDYPTVDD
                     YLISDIHPLHLEEDLGSIMMHVRGQEIPVFPVTDRNGIYAGVVELGILKELLLGEELK
                     NLVIVQDLVNRNYPFVYRDEPLDRVFDRFRENVCPALPVLEASETGDYYLGLLLLKDL
                     LPELTIEGGRAFRNRSG"
     gene            complement(843..3272)
                     /gene="leuS"
                     /locus_tag="PKN80_03945"
     CDS             complement(843..3272)
                     /gene="leuS"
                     /locus_tag="PKN80_03945"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNQ35198.1"
                     /translation="MNYDFKEIEARWQRTWEEQGLFRARPEGKKSYILEMFPYTSAQI
                     HMGHVRNYSIGDVIARQKMMQGFSLLHPIGYDSFGMPAENAAIKQKTQPSTWTYQNIA
                     TIRRQLKRLGLSYDWSREVITCDPDYYRWNQLVFLKLYERGLAYRKRSTANFCPACRT
                     VLANEQVINGGCWRCASPVSQKELEQWFFRITAYAEELLDSSGLGGWPERVRTMQANW
                     IGKSQGVEIFFPVSELHEKLTVFTTRADTIFGATYVVLSPDHPLIAGLAARSGRRDEI
                     EHFLSEWRRTKDDFDVEKFGVDTGFKAENPVNGEAIPIWIANYVLSGYGTGAIMAVPA
                     HDSRDFEFAVRYGLPVRRVICPPESGTVPASLETAYEEPGRLVNSGPFDGRESEAAKT
                     EIAAWMESKGIGRRTVHYRLRDWCISRQRYWGSPIPIVYCDKCGTVPVPEKDLPITLP
                     DAGVRFTGQGGSPLEIDSFVNCDCPKCGGRARRETDTMDTFVDSSWYFLRYASGDWNG
                     KPFDSGEVNFWMPVDQYIGGIEHAILHLLYSRFFTRVLRDIGLVSFSEPFSNLLCQGM
                     VIKDGAKMSKSKGNVVDPEEMINRYGADTIRLFILFAAPPEVDLEWSDQGVEGCWRFL
                     NRVWRLAEDAADYRPDRVDPAAEKDLVYHRHLTVKRVSEDSFVSFHFNTAIARLMEFS
                     NQLLEKKNGHRISREAFQEGVQVLVRLLAPFCPHLGEELWGRLNGQGSVFKAGWPDFS
                     ESALHQETMELVIQINGKNRSRMTVAAGTGQPEIEKLALADEKIRSGLAGRQVRRIIL
                     VAGKLVNIVAG"
     gene            complement(3269..4099)
                     /locus_tag="PKN80_03950"
     CDS             complement(3269..4099)
                     /locus_tag="PKN80_03950"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNQ35199.1"
                     /translation="MKFGVHLATAGRLTSVFENARELSCDTIQIFSRNPRGWFARPLS
                     ATEASDFKSARAAASISPLVVHAPYLINLAAPDPDIFDKSVRSCRQELERCALLGADY
                     FVLHPGSHRGEGLEAGIARAAGALHSILKETDSRVLLLLENTAGSGYSLGGRLEELAA
                     LMGDNPAIGFCFDTAHALAAGYDIAREPGLSSFLNSVDRLFGPGRMRLVHANDSRGGP
                     GSRLDRHADIGEGCIGLEGFARIVNHQRLQALPFILETPKEDLETDRRNLNTIRSLVK
                     "
     gene            complement(4096..4341)
                     /locus_tag="PKN80_03955"
     CDS             complement(4096..4341)
                     /locus_tag="PKN80_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35200.1"
                     /translation="MESNRPYWIILVVLAVAFLFFMHSIHGLRVEKVMKEAARYNEIH
                     IVWDWNAFQAWTDRGQTAKTFDEREKKLEEAETSGRR"
     gene            complement(4355..4430)
                     /locus_tag="PKN80_03960"
     tRNA            complement(4355..4430)
                     /locus_tag="PKN80_03960"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4395..4397),aa:Ala,seq:ggc)
     gene            complement(4548..5573)
                     /gene="mltG"
                     /locus_tag="PKN80_03965"
     CDS             complement(4548..5573)
                     /gene="mltG"
                     /locus_tag="PKN80_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNQ35201.1"
                     /translation="MLNSQPRSRGLIRLAAAIVGLPLLFLLLMTAYYFLPTAARTDTV
                     RVQPDSNLRQIVDRLYAKKLLRFPNLFVLTVRLKKEERKLQAGLYEIKAGSSPRLILH
                     KLLKGETATVRVTIPEGWSSREIGELLERKDLCPRESFLSVVEARQLEGFLFPNTYFV
                     PSQFTAEGIALLFVKGFESFWSADYETRARAQNLSVRSIITLASIIEREAKLDSEKPI
                     ISSVFYNRLRRGMKLEADPTILYALGSWNIRLTRRQLFTRSPYNTYLHRGLPPGPICN
                     PGKTSLLAALNPARTNYLFFVANGDGTHIFSRTGSEQAAAIRMIKARQAASQEKPDTG
                     LEPDGSE"
     gene            5741..6001
                     /locus_tag="PKN80_03970"
     CDS             5741..6001
                     /locus_tag="PKN80_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35202.1"
                     /translation="MWALIGGIIAVVLGVLLAALRWWQATKYVITGGIVILLVLGGLV
                     AVASGISDIKDRLAEKKEKEQEKEKESAQGPESAEKPAAPKE"
     gene            6013..6086
                     /locus_tag="PKN80_03975"
     tRNA            6013..6086
                     /locus_tag="PKN80_03975"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6047..6049,aa:Arg,seq:tcg)
     gene            6108..7262
                     /gene="alr"
                     /locus_tag="PKN80_03980"
     CDS             6108..7262
                     /gene="alr"
                     /locus_tag="PKN80_03980"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNQ35203.1"
                     /translation="MKTRSWIEVNLANVKENLQTVRDRVKPSRILACVKANAYGLGAV
                     EIARSLEADTAGFAVATVAEGLELRRAGIKKEILVLGCSAGPEEWPDALDRDLSLTIA
                     SRKQAEGISGLSARLKKRARVHLKVETGMNRLGVSPDEFQPLLNTCLSRPELTLAGIF
                     THLAAAETRNRFTLKQIELFRRLTERPGGLRPIPARHAANSAAVVNYPESYFELVRPG
                     ICLYGAYQGPVNRSGGKQPRFQPVASLKSRVVLVKRVRSGESIGYERLFRARRDTDVA
                     TVAIGYADGYARSISGRGRVIIRGKRRRILGLICMDMLMADVTGSGASVGDEVVLLGR
                     QGGTSISAAEVADWSGGITHEFLSRLGPRLERLYFNSRTTPERGAGLLPS"
     gene            7259..8575
                     /gene="purB"
                     /locus_tag="PKN80_03985"
     CDS             7259..8575
                     /gene="purB"
                     /locus_tag="PKN80_03985"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNQ35204.1"
                     /translation="MIKRYSNPEMARIWTETRKLQKWLEVELAVVEAWSRLGRVPETA
                     AAEIRRRARVNTRRVAELESVSNHDVVAFIEAVSETIGPLSSFFHLGLTSSDLLDTAN
                     ALRLMEAADRIKSGLEEFRVVLADQAQRHRKTLQVGRTHGIHAEPISFGLKLCGFYTE
                     TERSLERIARAREVIAYGKISGAVGTYAHLSPRLEEMVCRQLGLKSEPVSTQVVPRDR
                     YAEYLCQLAIIGTGLERFALEIRHLQRTEVGEAAEPFGRGQKGSSAMPHKQNPILCER
                     ICGLARLLRAYCQAGLEDVALWHERDISHSSVERVVLPDATITLDYLIRLSIRVAGGL
                     RVFPERMAANLDRTGGLIYSQRLLSALVGQGLPRPEAYRLVQSNALSAIRAGRDFREL
                     VRHDPEISRRLKPAELASLFEPGYFLRHQDEIYRRVFPRTVNKKEK"
BASE COUNT         1801 a         2552 c         2605 g         1633 t
ORIGIN      
        1 cgaaaggcac gggaactctc ggtggagaat caggcggtcc gcctggaaga gatcctgaca
       61 gcggcggtcg aaggacgtcc ttcttaaccg gaacggttgc gaaaagcccg cccgccctcg
      121 atcgtcaact ccggcaaaag gtccttgagc agaagcagcc ccaagtagta gtcccccgtt
      181 tcggaagctt caaggacggg cagggctgga cagacatttt cccggaaacg gtcgaagacc
      241 cggtcgagcg gctcgtcccg ataaacgaac gggtaattcc ggttcaccag atcctggacg
      301 ataaccaggt tcttcaactc ctccccgagc agcagttctt tcaaaatccc cagctcgacc
      361 actcctgcgt agatcccatt ccggtcggtg accgggaaaa ccggtatctc ctgcccccgg
      421 acatgcatca tgatcgaacc gagatcctcc tcgagatgaa gcggatgaat atcggaaatc
      481 aggtagtcgt caaccgtcgg ataatcgcca agcctttttg aatccggata aagaagacgt
      541 ttgctgacca acggccccag gaaggtggtg accagggaaa gcacgatgat cgagtcgagg
      601 atgacatcgg aaatcaaacc gatctcgtgg ccagcaaccg cggccgaccg ggtggccgaa
      661 aactggggta tagaaataat tcagcgaggc ggtctcgccg atgaacccga aaccgctcgg
      721 tcccaaaaac aggccgacca ggatgtaaaa ggaaagaacg ggcagtttta aaaggcggga
      781 gagcggccgg gctaccagca tcaccaggaa ggcggccagc aggtaaagca gggttgtttc
      841 catcaacccg cgacgatatt gaccagtttt ccggctacca ggatgatccg gcgcacctgg
      901 cggccggcca gtccggaccg gattttttca tcggccaggg ccagtttttc tatttcgggc
      961 tgcccggttc cggcggcgac ggtcatgcgg ctccggttct tgccattgat ctgaatgacc
     1021 agttccatcg tctcctggtg caacgccgac tcggagaagt ccggccagcc ggccttgaag
     1081 accgaaccct ggccgttcaa ccggccccag agctcctcgc ccaggtgcgg acagaagggc
     1141 gccagcagtc gaaccagaac ctggactcct tcctggaaag cttcccggct gatccggtgg
     1201 ccgttcttct tttcgagcag ctgattgctg aattccatga gccgggcgat ggccgtgttg
     1261 aagtgaaagg agacgaagga atcctcgctg acgcgcttga cggtcaggtg ccggtgatag
     1321 accagatcct tttcggcggc cgggtcaacc cggtcgggcc ggtaatcggc ggcatcctcc
     1381 gccagacgcc agactcggtt caggaaacgc cagcagcctt ctacgccctg atcgctccat
     1441 tccaggtcaa cttccggcgg cgccgcgaag aggatgaaga gacggatcgt gtcggccccg
     1501 taacgattta tcatctcctc gggatcaacc acgttcccct tcgacttgga catcttggcg
     1561 ccatccttga tgaccattcc ctggcagagc agattggaaa agggttcgct gaaggagacc
     1621 agcccgatgt cgcgcaggac ccgggtgaag aagcggctgt acagcaggtg gagaatggca
     1681 tgctcgatgc cgccgatata ctgatccacc ggcatccaga aattgacctc cccggaatca
     1741 aaaggtttcc cgttccagtc tccggaagca tatcggagga aataccagga ggagtcaaca
     1801 aaggtgtcca tggtgtcggt ctcgcggcgg gcgcgtccgc cgcatttcgg gcagtcgcaa
     1861 ttaacaaagg aatctatttc cagcggcgag ccgccctggc cggtaaaacg cactccggca
     1921 tccggaagcg tgatgggcag atccttttcc ggcaccggga ccgttccgca cttatcgcag
     1981 taaacaatgg gtatcgggct gccccagtaa cgctggcgcg agatgcacca gtcgcgcaac
     2041 cggtagtgga cggtgcgccg gccgataccc ttcgattcca tccaggcggc gatttcggtc
     2101 ttggccgctt cgctttcccg gccgtcaaac ggtccggaat tgaccaggcg gcccggctcc
     2161 tcataagcgg tctccaggct ggccggtacc gttccggact cgggcgggca aataacccgg
     2221 cgaaccggca gcccgtagcg gacggcaaac tcgaagtccc ggctgtcgtg ggccggcacg
     2281 gccatgatgg ccccggtgcc ataaccggaa aggacataat tggctatcca gatcggaatc
     2341 gcttccccgt tgaccgggtt ctcggccttg aatccggtgt ccacgccaaa cttttcgaca
     2401 tcgaaatcat ccttggtgcg gcgccattcg gacaaaaaat gctcgatttc atcacgacgt
     2461 ccggaacgag cggccaggcc ggctatcagg gggtggtccg gagaaagaac gacgtaggtg
     2521 gccccgaaga tggtgtccgc ccgggtggta aaaacagtta gtttttcgtg cagttccgaa
     2581 accggaaaga agatttcgac tccctggctt ttgccgatcc agttggcctg catggtccgc
     2641 actcgttccg gccagccgcc gagaccgctg gaatccagta actcctccgc gtaggcggtg
     2701 atccggaaga accattgttc cagctccttc tggctgaccg gagaggcgca tctccagcag
     2761 cccccgttaa tgacctgttc gttggccagg accgtccggc aggcgggaca gaagttggcg
     2821 gtcgagcgtt tccgataggc caggccgcgt tcatacaatt tcagaaagac gagttgattc
     2881 cagcggtaat aatcgggatc gcaggtgata acctcgcggc tccagtcgta ggaaagcccg
     2941 agacgcttga gttgccgccg gatggtggcg atgttctgat aggtccaggt ggaaggctgg
     3001 gtcttctgct tgatggccgc gttctcggcc ggcattccaa aggaatcgta accgatcggg
     3061 tgcagcaggg agaatccctg catcattttc tgccgggcaa tgacgtctcc gatgctgtaa
     3121 ttgcggacat ggcccatgtg gatctgggcg ctcgtgtagg gaaacatctc cagaatataa
     3181 cttttcttcc cctcggggcg ggcccggaag aggccctgtt cttcccaggt ccgctgccag
     3241 cgggcttcga tctctttgaa atcgtagttc attttaccag tgacctgatt gtgttgaggt
     3301 tgcgccggtc cgtttccagg tcctccttgg gcgtttccag gatgaagggc agcgcctgca
     3361 aacgctggtg attgactatt ctggcaaagc cctccaggcc gatacaccct tccccgatgt
     3421 cggcgtggcg gtccagccgg ctccccggcc cgccgcgtga atcgttggcg tgtaccaatc
     3481 gcatccgacc cggcccgaaa agacggtcaa ctgaattcag gaaagaggaa agtcccggtt
     3541 cccgggcgat atcgtaaccg gccgccaggg cgtgggcggt gtcaaagcaa aacccaatcg
     3601 ccggattgtc acccatcaac gcggcgagtt cttcaagacg tccgccaagg ctgtacccgc
     3661 tgccggcggt gttttccagc agcagtagca cccgggaatc cgtttccttc agtatggaat
     3721 gcagcgcccc ggcggcccgg gcgatccccg cctccagacc ttctccccgg tggctgcccg
     3781 ggtgcagcac gaaatagtcg gcccccagca gtgcgcatct ttcgagctcc tggcggcagg
     3841 aacggactga cttgtcgaaa atgtccggat ccggagccgc gagattgatc aggtaagggg
     3901 cgtggaccac caggggagag atcgaagctg ccgcccgggc gcttttgaaa tcggacgcct
     3961 cggttgcgga aagcggccgg gcgaaccaac cgcgcgggtt gcgggaaaat atctggatgg
     4021 tgtcgcagga aagttcgcgc gcgttttcaa aaacgctcgt caaccggccg gcggtagcca
     4081 ggtgcactcc aaacttcatc ggcgtccgga ggtctcggct tcctcgagtt tcttctcccg
     4141 ctcgtcgaat gtcttggcgg tctgaccgcg gtcggtccac gcctggaagg cattccaatc
     4201 ccagacaatg tgaatctcgt tgtaacgggc cgcctccttc atgacctttt caacccgcaa
     4261 accgtggatt gagtgcatga agaaaaggaa ggccaccgcc agcaccacca ggatgatcca
     4321 gtaaggccgg ttcgattcca tgtcatctcc agattggtgg aggataaggg atttgaaccc
     4381 ttgacctctt gcatgccatg caagcgctct cccaactgag ctaatccccc aaaacggcaa
     4441 gaattatact acggggcgcc atcgcacgtc aacggcctga tgcacgggac gccgggtaat
     4501 ggcggcgatc ccggcgcctt tccccggccg aagattgaaa accgccccta ttcagacccg
     4561 tccggctcaa gaccggtgtc cggcttttct tgcgacgcgg cctgccgggc cttgatcatt
     4621 cgaatcgccg cggcctgctc cgaaccggtc cgggaaaaga tgtgggtccc gtcaccgttg
     4681 gcgacgaaga acaggtagtt ggtgcgagcc gggttgaggg ccgccagcag ggaggtcttg
     4741 ccggggttgc agatcgggcc gggcggaagt ccccgatgca aataggtgtt ataaggggaa
     4801 cgggtaaaga gctgacgcct ggtaagcctg atgttccagg atccgagggc gtaaagaatc
     4861 gtcggatcgg cctccagttt catgccgcgg cgcagccggt tgtaaaaaac ggaacttatg
     4921 atcggcttct cggaatccag tttggcctcg cgctcgatga tcgaagccag cgttattatg
     4981 ctgcggaccg aaaggttctg cgcccgggcc cgcgtttcgt aatcggccga ccagaaagac
     5041 tcgaatcctt tcacgaagag cagggcgatt ccttcggcgg tgaactggga cggcacaaag
     5101 taggtgttgg gaaaaagaaa cccctccagc tgccttgctt ccaccacgga cagaaaactt
     5161 tcgcgcggac aaagatcctt gcgttccagc agttcgccta tttcgcgcga actccagcct
     5221 tcgggtatgg tcaccctgac ggtggccgtc tcacccttca gcagtttgtg caggatgagg
     5281 cgcggactgc tgccggcctt gatttcgtaa agtccggcct ggagttttct ttcctctttt
     5341 tttaaacgga ccgtcagcac gaagagattc gggaaacgca acaatttctt ggcgtagagc
     5401 cgatcgacga tctggcggag atttgaatcc ggctgcaccc ggaccgtgtc ggtccgggcc
     5461 gccgtcggca gaaaataata ggcggtcatc agcagcagga ataaaagtgg caggccgaca
     5521 atggcggcgg ccagccggat caatccccgg gaacgtggtt gggagttaag cataattgtt
     5581 ttaggttata ttttactcca gccggcttat ccgcgccaat ccggcttgac gctcccgggc
     5641 ggttatgcta tagtatgaac accttgaaag aggcattatt gctggaaatt ttaggttgaa
     5701 ggccggataa ttaccggccg gttttcaata aggagggagc atgtgggcat tgatcggggg
     5761 tatcatcgcg gttgtcctgg gcgtgctgct ggcggccctg cgctggtggc aggcgaccaa
     5821 gtacgtaatt accggcggca ttgtcatcct gctggtgctt ggcggcctgg tggccgtggc
     5881 ctcgggaatc agcgacatca aggaccggct ggccgagaaa aaggaaaagg aacaggagaa
     5941 ggaaaaagag agcgcccagg gaccggaatc cgctgaaaaa ccggcggcgc cgaaagaata
     6001 aactcgcctc ttgcgcctgt agctcaaccg gatagagcgc cgggcttcga acccgtaggt
     6061 tgggcgttcg agtcgcccca ggcgcgccga agcatggcca agcacaagtg aaaacccggt
     6121 cctggataga agtcaacctg gcaaacgtaa aggagaacct ccaaacggtg cgagaccggg
     6181 ttaagccgag ccggatcctg gcttgtgtca aggccaacgc ttacggtttg ggcgccgtcg
     6241 aaatcgcccg ttccctggaa gcggacacgg ccgggttcgc ggtggccacc gtagcggagg
     6301 gactggaact gagacgggcc ggcatcaaga aggaaatcct ggtcctggga tgcagtgccg
     6361 gccccgaaga atggccggac gccctggacc gtgatctcag cctgaccatt gcttcgcgga
     6421 aacaggcgga aggcatctcc ggattatcgg cccggttgaa aaaacgggcc cgggtccacc
     6481 tgaaagtgga aaccggcatg aatcggctgg gggtatcccc ggatgaattt caacctcttt
     6541 tgaatacctg tctctcccgg cccgagttga ccctggccgg catcttcacc cacctggccg
     6601 ccgccgaaac ccggaaccgt ttcacattga aacaaatcga actttttcgc cggttaaccg
     6661 aaagaccggg cggactccgg ccaattccgg cgcgccacgc cgccaactcg gcggccgtgg
     6721 taaattatcc ggaaagttac tttgagctgg tccggccggg tatctgcctt tacggggcct
     6781 accagggacc ggttaaccgc tccggcggaa aacagccccg cttccaaccg gtcgcaagcc
     6841 ttaaatcgcg ggtggtgctg gtcaagcggg tccgttcggg cgaatcgatc ggttacgagc
     6901 gtcttttcag ggcccgccgc gataccgatg tcgcgaccgt ggccatcggc tacgccgacg
     6961 gatacgcccg atccatctcc ggccggggcc gggtaatcat ccggggaaaa cggcggcgga
     7021 ttctgggact gatctgcatg gacatgctga tggccgacgt taccggaagc ggagcttcgg
     7081 tcggtgatga ggtggtcctg ctcggccgcc agggcggaac aagcatctcg gccgccgagg
     7141 tggccgattg gagcggcggg atcacgcacg aattcctgag tcggctggga ccgcgcctgg
     7201 aacgccttta tttcaacagc cggacgacgc cggaaagggg cgccggtttg cttccatcat
     7261 gattaaaaga tacagcaacc ccgaaatggc gcggatctgg accgaaacgc gcaagttgca
     7321 aaagtggctt gaagtcgaac tggccgtggt ggaagcctgg tcccggctgg gaagggtgcc
     7381 ggaaacggcg gcggcggaaa tccgccggcg ggcccgggtc aacacccggc gcgtggccga
     7441 actggagtcg gtctccaacc acgacgtggt ggccttcatc gaggcggtca gcgaaacgat
     7501 cggacccctt tcatccttct ttcacctcgg cctgacttct tccgacctgc tcgacaccgc
     7561 caacgccctg cggctgatgg aagcggccga ccggatcaaa tccgggctgg aagagttccg
     7621 cgttgttctg gccgaccagg ctcaacgcca ccgaaaaaca ctccaggtgg gtcgaacgca
     7681 cggaatccac gccgaaccaa tcagtttcgg cctcaagctc tgcggttttt acaccgaaac
     7741 cgaacgctcc ctggaacgga tcgcccgcgc ccgggaagtg atcgcctacg gaaaaataag
     7801 cggggcggtt ggcacttacg cccacctgtc tccccggctc gaagagatgg tctgccgcca
     7861 gctcggcttg aaatcagaac ccgtctcgac ccaggtggtt ccccgcgacc gctacgccga
     7921 atacctgtgc cagctggcca tcatcggaac cgggctggag cggttcgctc tcgaaatcag
     7981 gcacctgcag cggaccgagg tgggcgaagc cgccgaaccg ttcggacgcg gtcaaaaagg
     8041 ctcctcggcc atgccgcaca agcagaatcc gatactctgc gaacggatct gcggattggc
     8101 caggctcctg cgggcttact gccaggccgg cctcgaagat gtggcactct ggcacgagcg
     8161 ggatatcagc cactcgtcgg tcgagcgcgt ggtcctgccc gatgccacca taaccctgga
     8221 ctatctcatc cggctgtcca tccgggtggc cggcggcctg cgggttttcc cggaaaggat
     8281 ggcggccaac ctggatcgca ccggaggcct gatctattcg caaagacttc tgtcggccct
     8341 ggtcggacag ggcttgcccc gacccgaggc ctatcgcctg gtccagtcca acgccttgtc
     8401 cgccatccgc gccgggcgtg acttccggga gctcgtccgg cacgatcctg aaattagccg
     8461 ccgcctcaag ccggccgaac tcgcttctct ctttgaaccc ggatacttcc tgcgccacca
     8521 ggatgaaatc tatcggcggg tttttccgcg caccgtcaac aaaaaagaaa aatgagcaac
     8581 gacgatctgc a
//
LOCUS       DAOQYN010000069         8464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8857_length_8464_cov_4.318349, whole genome shotgun sequence.
ACCESSION   DAOQYN010000069 DAOQYN010000000
VERSION     DAOQYN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8857_length_8464_cov_4.318349"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PKN80_03990"
     CDS             <1..1021
                     /locus_tag="PKN80_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35205.1"
                     /translation="DPWLRSVERELRRILIKHEIGDDSPVADYFPVAAVFKVTPSDTW
                     GVRIEHHRPSEAGGSWKFSPPLKDEADFERLRMPRYTYDSAATESRLAALDKLFGDRL
                     PPRYVCHLPLSATLGTVAADLRGLTELMLDMAVRPDLAHRLMALMRDACLASLDDLEA
                     AGRLTPNNDEAMTGSDPVGPEIPADGRFSLKNLWIMANSQEFDQVSPAMWREFCLEYQ
                     RPILERFGLSAYGCCENLTRKIDGVLSIPNLRIFVCSAWTDLDRVIERVERNYVIMWR
                     QKASEVVFAPDTDRLRQDLESGLQRLKGAYYQIVLREIQTLAGHLDRLHLWTRLAIES
                     AEKHA"
     gene            1014..1940
                     /locus_tag="PKN80_03995"
     CDS             1014..1940
                     /locus_tag="PKN80_03995"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNQ35206.1"
                     /translation="MPEIFVPENLPIRFILSKAAGGQRAFRLERRISKFLSGTGREFF
                     FYRVEAPGQAASIARRLAAESRGPVVAVGGDGTVNEVINGIYGSGVTLGILPMGSGNG
                     LAASLEIPRRLKAAVRVIAAGRVRRIDLARIKTGDRESYFAATLGMGMDPLIAGQVCS
                     FRTAIRGPWLYFLAGLRAYFLFTSRYRQVRLEGELDGRLLRQTCLLACVANVPQYGAG
                     ARIAPAARVDDGRLDLVLLPNAGFRRSFAYSLALFTGRLDRQAGVETQRFRRLLFREL
                     APDFLQADGDPLKLPLPFSVEVVPAALPVFSG"
     gene            complement(1993..3690)
                     /locus_tag="PKN80_04000"
     CDS             complement(1993..3690)
                     /locus_tag="PKN80_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ35207.1"
                     /translation="MKRKWLGLAMAASMLLAGTAAGAATLVDSFEKGYQYDRDLHSQQ
                     GWQASRTGVAYEGLVTLTDREAKTGKYSVWLRPKSPDGKNIGMSRTLDFQKEVGWIDF
                     WLKCAYGPSSRILVSVSGENFSAVAGAQPGFVYGGDTVEYNRIPADRVPVKDKQYFKP
                     NEWTRFTLRTDSRQGRFDLYLNGRLALKDLQANKNGGPGNIKNLSIALRAYGTPEELA
                     RDQVFIDDLYLSDEMPPTNLVISADGSFAVAHASSSSAEAAVSVSVSPSGASAVSASG
                     GGAVQPTISIDGKVVQSSSTNLVISDQPFFPVRPKEVLFRFVHISDSHARADGTSMME
                     LNRVVNDINRMGVDFVLHSGDLADAGNIKGTEMALAELKRLKMPFYLTPGNHEKFGEV
                     GKAGRYEEMTGFKPYYSFEYKGFLFVALDTPVAEPDHMGALDEAQLTWLEKELQKGKP
                     TVIFTHVPLYGTYPQGSQAYEIKGPSREKLEALLAKYKPAAFLAGHLHRVNLFKETEN
                     TFFSVAPALTPLYNWAPGKGYVIWDVYPDKVAQHYQYIYYPYVKEEGRTYGESKFKK"
     gene            3848..5731
                     /locus_tag="PKN80_04005"
     CDS             3848..5731
                     /locus_tag="PKN80_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNQ35208.1"
                     /translation="MSISLQPVDFASVRFTGGFWSERLRVNRRVSVPLVYRRLQETGR
                     IGAFNLDWRPGREPVPHHFWDSDVYKWVEAASYVLAERSNPSLAARLQELAGRIAAAQ
                     QPDGYLNVYYTVVEPKLRWTNLMRSHELYTAGHFFEAAVAYHRATGDSRLLEAAQRFA
                     DLIGRVFGPGPEQRPGYDGHPEVELALVGLYRATGRRRYLELARFFLEERGRPPHFWD
                     RERRERNDPAEPEPVPYERLQAHRPLLEQTEAVGHAVRAVYLYSGLADLAAETGDRRY
                     RRTLDRLWESVALRKLYLTGGIGALAVGEQFSGDYHLPNETAYAETCASIGLIFWARR
                     LLDLDCDRRYAEVMEAALYNGALSGVSLDGRRFFYVNPLASEGRHHRQEFFGCACCPP
                     NLLRLLASLGRYFYSAAEDELCVHLYGAGAARLEISGVRLRFVQETDYPWEGRVGLEI
                     SPERPVRFRLRLRVPEWCRNWSLSINGTSLSGRLSRGYLLLDRTWRPGDRLALDLEMK
                     PELVHAHPAVGTAAGCAALRRGPLVYCLEGVDNPGLPSIFIDSARSFRPVRVESGPLK
                     GLVLLKGRGWRASRAGWEKRLYSDRAPKLEPAPVIAVPYFVWDNRAAGPMRVWLPRPP
                     AGS"
     gene            complement(5728..6117)
                     /locus_tag="PKN80_04010"
     CDS             complement(5728..6117)
                     /locus_tag="PKN80_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNQ35209.1"
                     /translation="MKNEIRRIETGTAPRAIGPYSQAVACGNLVFTSGQISIDPATNQ
                     FTGGDVSEQTRRALENLKAVLAAAGCGLERVVKTTVYLKDMKDFEAMNRVYAEYFTEH
                     PARATVQAAGLPKNALVEIDCLAVRPD"
     gene            6304..7254
                     /locus_tag="PKN80_04015"
     CDS             6304..7254
                     /locus_tag="PKN80_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HNQ35210.1"
                     /translation="MNLMSTLKGSLMEGFLPAGWDLARIDRCASQPPDEILKRQPWWQ
                     RDFQPVACASLEEFGVLMGHEIALEICRAAEEKRELILILPVGPMGMYRWTVHFLKEW
                     GVDCRHVHGFNMDEWSDRAGNTLPADHPGAFQKAMEDAFYGPLGRLGVPRKQRHFARR
                     DQLPAYPEEINRLRKRGARMSVVFGIGRVFHIAFWEPHFAAEFKSEAAWRRAEYRLAA
                     RLHPLTIEQNAITSFKSRTTLVPAFANTIGPGIFLKADRIIGGCDGALGRGMMWQGMS
                     LWVTLRYGPDIWVPSSFMPTRPGRLFFLKELAGPLTAECN"
     gene            7267..7968
                     /locus_tag="PKN80_04020"
     CDS             7267..7968
                     /locus_tag="PKN80_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNQ35211.1"
                     /translation="MNILAVGAHPDDLEFLCAGTLARYAAAGHRVFMAHLCSGNKGGR
                     EIDPAELAAVRDREATAAAAVIGAETLGPVAADLDLYPDRETRQAVVEIIRTAKPDLI
                     FTHDPGDYMPDHVNTSRLVFDAAFTATLPLHRSGRPAHEAIVPIYYLDTMSGVGFAPE
                     EYVDITGFFETKKRMLLAHESQYKWVQGHHLTDPLRLLEITGRYRGLQCGVEYAEAFR
                     AARVWGRLRPERLLP"
     gene            8183..>8464
                     /locus_tag="PKN80_04025"
     CDS             8183..>8464
                     /locus_tag="PKN80_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNQ35212.1"
                     /translation="MPISIGLEEVQRARALISGRVVRTPLVEFPAPELGGPIGLKLEN
                     LQHTGAFKVRGVLNKIASLTGEERRRGVICATSGNHGLGVAHVAHREGLR"
BASE COUNT         1362 a         2745 c         2729 g         1628 t
ORIGIN      
        1 tgatccctgg ctgcgttcgg tggaacggga gctgcgccgg atactcatca agcacgagat
       61 cggcgacgac tcgccggtgg ctgattactt cccggtggcg gccgttttta aggtaacccc
      121 gtctgacacc tggggagtca ggatcgagca ccatcgtccc agcgaggccg gcggctcctg
      181 gaagttctcc ccgccgctca aggacgaggc cgatttcgag cgcctccgga tgccccgtta
      241 tacctacgat tccgccgcca cggagtccag gctggcggcc ctggataaac ttttcggcga
      301 ccggctgccg ccacgttacg tctgccacct cccgctttcg gccactctgg gcaccgttgc
      361 ggccgacctg cgcggcctga ccgaactgat gctcgatatg gccgtccggc ccgacctggc
      421 gcaccggttg atggccttaa tgcgggatgc ctgcctggct tcgcttgacg acctggaggc
      481 ggccggccgg ctcaccccga acaacgacga agcgatgacc ggcagcgatc cggtcggccc
      541 ggagatcccg gccgacggcc ggttttcact gaagaacctc tggatcatgg ccaacagcca
      601 ggagttcgac caggtgtcgc cggccatgtg gcgggagttc tgcctggagt accagcggcc
      661 catcctggag cgtttcggcc tttccgccta tggctgctgc gagaacctga cccgcaagat
      721 agacggtgtt ctttccatcc ccaacctgcg tatcttcgtc tgcagcgcct ggaccgacct
      781 ggaccgggtc atcgagcggg tggaacggaa ttacgtcatc atgtggcggc agaaggccag
      841 cgaggtggtc ttcgccccgg acaccgaccg gcttcgccag gacctggaaa gcggtctgca
      901 gcgattgaag ggcgcttatt accagattgt gctgcgggaa atccagaccc tggccggcca
      961 cctggaccgg ctccatctct ggacacgcct ggccatcgag tcggctgaaa agcatgcctg
     1021 agattttcgt gccggaaaac cttccgatcc gttttatcct gagcaaagcg gccggcggcc
     1081 agcgggcttt tcgtcttgag cgccgcatct ccaaatttct gtccgggacg gggcgggagt
     1141 tttttttcta ccgggtcgag gcgccgggcc aggccgcttc catcgcccgg cggctggcgg
     1201 ccgaaagccg gggcccggtg gtggccgtcg gcggtgacgg taccgtcaac gaggtcatta
     1261 acggaattta cggttccggg gtgacgctgg gaatacttcc gatgggcagc ggtaacggac
     1321 tggcggccag tttagagatt ccccgccgcc tgaaggcggc ggtccgggtg atcgccgccg
     1381 gccgcgtccg ccggattgac ctggcccgga ttaagacggg agatcgggag agttacttcg
     1441 cggccaccct gggaatgggc atggatcctt tgattgccgg gcaggtctgc tcgtttcgca
     1501 ccgctataag gggtccctgg ctctatttcc tggccgggct gcgggcttac tttctcttta
     1561 ccagccgcta ccgtcaggta agattggagg gagagctgga tggacggctc ctgcgccaga
     1621 cctgcctgct ggcctgcgtg gccaacgtcc cgcaatacgg cgccggagcc cggattgctc
     1681 ccgccgcccg ggtggacgac ggccggctgg acctggtcct gcttcccaac gccggcttcc
     1741 gccgttcctt cgcttacagc ctggccctct tcaccggccg cctcgaccgc caggccgggg
     1801 tggagacgca gcgcttccgg aggctgctct tccgggaact ggccccggat ttcctgcagg
     1861 ccgacggcga tccgctgaaa ctgccgcttc ccttctccgt cgaggtggtg ccggccgccc
     1921 tgccggtttt ttccggttaa gcggccgcac ccggtcttcc cggcggagat aacctttggt
     1981 caatcccgcc gcctacttct tgaatttcga ctcaccgtag gtccgtccct cctccttcac
     2041 gtatggataa tagatgtact ggtaatgctg ggccaccttg tccgggtaaa cgtcccagat
     2101 tacatagccc ttgccgggtg cccagttgta aagcggagtg agggccggcg ccaccgagaa
     2161 gaaggtgttt tcggtctcct tgaagaggtt tacccggtgc aggtggccgg ccaggaaggc
     2221 ggccggcttg tatttggcca gcagggcctc cagcttctcc cggctcggtc ccttgatctc
     2281 gtaggcctgg ctgccctggg gataagttcc gtaaagcggc acgtgggtga agatgacggt
     2341 cggcttcccc ttctggagtt ccttctccag ccaggtcaac tgggcctcgt ccagcgcgcc
     2401 catgtgatcg ggttcggcca ccggcgtgtc cagggcgacg aagaggaatc ccttgtattc
     2461 aaatgagtaa tagggtttga agccggtcat ctcctcgtaa cgtccggcct tgccgacctc
     2521 tccgaatttt tcgtgattgc cgggtgtcag atagaagggc atcttgagcc gtttcaactc
     2581 ggccagagcc atttcggtgc ccttgatgtt gccggcatcg gccaggtcgc cggagtgaag
     2641 aacgaagtcg actcccatcc ggttgatgtc gttgaccacc cggttcagct ccatcatgga
     2701 agttccgtcg gcccgggcgt ggctgtcgga aatatggaca aaacggaaga ggacctcctt
     2761 cggccggacc gggaagaagg gctggtcgct gatgaccagg ttggttgaag aggactggac
     2821 gaccttgccg tcgatggaga tggtcggctg aacggcgccg ccgccggagg cggagacagc
     2881 cgaggcgccc gaaggcgaga cggaaacgct tacggccgcc tctgcggagg aagagctggc
     2941 gtgggcgacg gcgaaggaac cgtccgccga gatcaccagg ttcgtcggag gcatctcgtc
     3001 gctcaggtag aggtcatcga tgaagacctg gtccctggcc agttcttccg gggtaccgta
     3061 agctcgcagg gcgatgctga gattcttgat gttgccggga ccgccgtttt tgttggcctg
     3121 caggtccttc agcgccagcc ggccgttcag gtagagatca aaccggccct ggcggctgtc
     3181 ggtgcgcagg gtgaagcggg tccactcgtt cggtttgaaa tactgcttgt ccttgaccgg
     3241 cacccggtcg gccgggatgc ggttgtactc cacggtgtcc ccgccgtaaa cgaagccggg
     3301 ctgggcaccg gctacggcac tgaagttctc gccgctgacg ctgaccagga tccgcgacga
     3361 gggaccgtag gcgcacttca gccagaagtc gatccagccg acctccttct ggaagtcaag
     3421 cgtccgtgac atgccgatgt tcttgccatc cggcgatttc ggccgcagcc agaccgagta
     3481 cttgccggtt ttagcctccc ggtcggtcag cgtgaccaag ccttcgtaag ccaccccggt
     3541 gcggctggcc tgccagccct gctgcgagtg caggtcccgg tcatactggt aacctttttc
     3601 gaaggaatcc accagggtgg ccgctccggc cgcggttccg gccagcagca tcgaggccgc
     3661 catcgccaat ccaagccatt ttctcttcat gatgccctcc cttattaaaa acaaacggta
     3721 agttcgcctg cttttttcga ttatacttta attaagccgg cctggcaacc ggattttcat
     3781 tccggttgag gcggccgagt ggtataattt acaaaaatgt tttttccctt gatcaaggag
     3841 gccgcggatg tccatctcgc tccagccggt tgacttcgcc agcgtccgat ttaccggcgg
     3901 tttctggtcg gaacggttgc gcgtcaaccg ccgggtttcg gttcccctgg tttaccggcg
     3961 gctgcaggag accggccgga tcggcgcctt caaccttgac tggcggccgg gccgggaacc
     4021 ggtgccgcac catttctggg actccgacgt ttacaagtgg gtggaggcgg ccagttacgt
     4081 cctggccgag cggtccaacc cgtccctggc ggcccggctg caggaactgg ccggccgtat
     4141 cgcggccgcc cagcagcccg atggctacct gaacgtgtac tataccgtgg tcgagccgaa
     4201 actgcgctgg accaacctga tgcgcagcca cgaactctat accgccggcc acttcttcga
     4261 ggcggccgtc gcctaccatc gggccaccgg cgactcccgc cttctggagg cggcccaacg
     4321 tttcgccgac ctgatcggcc gggtcttcgg gcccgggccg gagcagcggc ccggttacga
     4381 cggccacccg gaagtggagc tggccctggt cggactctac cgggccaccg gccgccgccg
     4441 ttacctggaa ctggcccgtt tttttctgga ggaacggggt cggccgcccc acttctggga
     4501 ccgggaacgg cgcgaaagaa acgatccggc cgagccggaa ccggtgccct acgaaaggtt
     4561 acaggcccat cggccgctgc tggagcagac cgaggcggtc ggccacgccg tccgggccgt
     4621 ctacctttac agcggcctgg ccgacctggc ggccgaaacc ggcgaccgcc gctaccgacg
     4681 gaccctggat cggctctggg agagcgttgc gctgcgcaag ctctacctga ccggcggtat
     4741 cggcgccctg gccgtcggcg aacagttctc cggggattac caccttccca acgagaccgc
     4801 ctacgccgaa acctgcgcct ccatcggtct tatcttctgg gcccgccgtc tgctggacct
     4861 ggattgcgac cgccgttacg ccgaggtcat ggaggcggcc ctttataacg gggccctcag
     4921 cggcgtttcc ctcgacggcc gccgcttctt ctacgtcaat ccgttggcca gcgaggggcg
     4981 ccaccaccgg caggaattct tcggctgcgc ctgctgtccg cccaacctgc tgcgcctgct
     5041 ggcctccctc ggccgttact tctactcggc cgccgaagac gagctctgcg tacatcttta
     5101 cggcgccggc gcggcccggc tggagatctc cggagtgcgg ctgcggtttg tccaggagac
     5161 ggattatccc tgggaggggc gggtcggatt ggaaatttcg ccggagcggc cggtccgctt
     5221 ccggctgcgc ctgcgggttc cggaatggtg ccgaaactgg tccctgtcga tcaacggaac
     5281 gagtttgagc ggccgattga gccggggtta cctgctcctg gatcggacct ggcggccggg
     5341 cgaccggctg gcattggacc tggagatgaa gccggagctg gtccacgctc acccggcggt
     5401 cggcaccgcc gccggctgcg ccgccctgcg gcgcgggccg ctggtttact gcctggaggg
     5461 tgttgacaac ccgggcctgc cctcgatttt tattgattcg gcccggagtt tccggccggt
     5521 ccgggtggag tccggcccgc tcaagggttt ggtgcttttg aagggaaggg gctggcgcgc
     5581 ctcgcgggcg ggttgggaaa aacggcttta ctcggaccgg gcgccgaaac tggaaccggc
     5641 gccggttatc gccgtgccct acttcgtctg ggataaccgg gcggccggac cgatgcgggt
     5701 ctggctgccc cggccgccgg ccggctccta atccggccgg accgccaggc agtcgatctc
     5761 gaccagggca ttcttcggca gtccggccgc ctggacggtg gcgcgggccg ggtgttcggt
     5821 gaagtactcc gcgtagaccc ggttcatcgc ctcgaagtcc ttcatgtcct tgagatagac
     5881 cgtggttttg accacccgct ccaggccgca gccggccgcg gccaggaccg ccttgaggtt
     5941 ctccagcgcg cgccgggtct gttcagagac atccccaccg gtgaactggt tggtggccgg
     6001 gtcgatggag atctggcccg aggtgaagac caggtttccg caggccaccg cctggctgta
     6061 aggaccgatg gcccggggtg cggttccggt ttcgatccgg cggatctcat tcttcatggc
     6121 ggtctccctt tccaagttaa acccggttcg acgggctatt ttcctgccaa tcaaaacggc
     6181 ggccggcgcg cttcggcgtt ttttggccga cttgtcgcgc cctgcctttt ttgatattat
     6241 actctaaaga ttctccgtag agctgaaaat cagccttttg tgaacccttg aggagaaaaa
     6301 attatgaacc tgatgagtac cctgaaagga tccttgatgg aggggttttt gccggccggc
     6361 tgggatctgg ctcggattga ccgctgcgcc tcccagccgc cggatgagat cctgaagcgc
     6421 cagccctggt ggcagcggga cttccagccg gtggcctgcg cctccctgga ggagtttggc
     6481 gttttgatgg gccacgagat cgccctggag atctgtcggg ccgccgaaga aaaacgcgag
     6541 ctgattctca tcctgccggt ggggccgatg ggcatgtacc gctggacggt ccatttcctg
     6601 aaggagtggg gagtggattg ccgccacgtg cacggtttca acatggacga gtggagcgac
     6661 cgcgccggca acaccctgcc ggccgaccac ccgggcgcct tccagaaggc catggaggat
     6721 gccttctacg gcccgctcgg ccgcctgggc gtaccccgga agcagcgcca cttcgcccgg
     6781 cgcgaccagc tgcccgccta cccggaggag atcaatcgcc tgcgcaaacg gggggcccgg
     6841 atgtcggtgg tcttcggcat cggccgggtc tttcatatcg ccttctggga gccgcacttc
     6901 gccgccgagt tcaagagcga ggcggcctgg cgccgggccg agtaccggct ggccgcccgg
     6961 cttcacccgc tgaccatcga gcagaacgcg atcaccagct tcaagagccg caccacgctg
     7021 gtaccggcct tcgccaacac gatcgggccg ggcatcttcc tgaaggccga ccggatcatc
     7081 ggcggctgcg acggcgccct gggccggggc atgatgtggc aggggatgtc cctctgggta
     7141 accctgcgct acgggccgga catctgggtg ccgagcagtt tcatgcccac ccggccgggg
     7201 cgccttttct tcctcaagga actggccggt ccgcttacgg ccgaatgcaa ctgaccggag
     7261 aagccgatga atattttagc cgtgggcgcc catcccgacg atctggaatt tctctgcgcc
     7321 ggcacgctgg cccggtacgc ggccgccggc caccgggtct tcatggccca cctctgttcc
     7381 gggaacaagg gcggccggga gatcgacccg gccgagctgg ccgccgtccg cgaccgggag
     7441 gcaaccgccg cggccgccgt catcggcgcc gaaacgctgg gaccggtcgc cgccgacctt
     7501 gacctctatc ccgaccggga gaccaggcag gcggtggtcg agatcatccg gaccgcgaag
     7561 ccggacctga tcttcaccca tgaccccggt gattacatgc ccgaccacgt caacaccagc
     7621 cggctggtct tcgacgccgc cttcaccgcc accctgccac tgcaccgatc cggccggccg
     7681 gcccacgagg cgatcgtgcc gatctattac ctggacacca tgtccggcgt cggcttcgcg
     7741 ccggaggagt acgttgacat caccggcttc ttcgagacca agaaacggat gctgctggcc
     7801 cacgaaagcc agtacaagtg ggtccagggc caccacctca ccgatccgct ccggctgctc
     7861 gagatcaccg gccgctaccg gggcctgcag tgcggcgtcg agtatgccga ggcattccgg
     7921 gcggcccggg tctgggggcg gctccggccg gaacgtctcc tgccctgacg gccttcagac
     7981 gcggccgctc ggaaaggatg accggaattt tcgggaagtc acgccccttt tttattatag
     8041 gatccgggcg ggcgaacaag tttagacaaa agcaccttcc atattctatc attataagtg
     8101 gaagagtccg aaagatgact ccggccgcac tgcttgccct ggcctccggc cgccgttctt
     8161 tcccgaaaca ctgtccgttc cgatgccgat ctcaatcggt cttgaagagg ttcagcgggc
     8221 ccgcgcgctg atttccggcc gggtggtccg cacgccgctg gtcgaattcc cggccccgga
     8281 gctcggcggc ccgatcggcc tgaagctgga aaaccttcag cataccggcg ccttcaaagt
     8341 gcggggcgtg ctcaacaaga tcgcttcgct caccggggaa gagcggagga ggggtgtcat
     8401 ctgcgccacc tccggcaacc acggcctggg cgtggcccac gtcgcgcacc gggagggact
     8461 gcgg
//
LOCUS       DAOQYN010000070         8455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_8866_length_8455_cov_4.539643, whole genome shotgun sequence.
ACCESSION   DAOQYN010000070 DAOQYN010000000
VERSION     DAOQYN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8866_length_8455_cov_4.539643"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="PKN80_04030"
     CDS             complement(<1..1521)
                     /locus_tag="PKN80_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35213.1"
                     /translation="MKRKNILAGLLLLAVLIPLSAAAENLLRDASFEAGAGPLAWEVI
                     GLDAGGPIGRANLDTSTAVHGRTSLKLPLLARPLFSGPVRIPAAGDYVFSVQLKASQP
                     VQVQLMAESEKSAGAIQSGTMYGQARFNVTAEWQRFDLKVKLPKEIVNVRITPLSAAA
                     GGHLWVDAAALTAGESPQPFSPRRPLEAGLTCEVPAKVFYPGEAGAVELWVANHTAGQ
                     ETPRFRYTVTDIYDRKGPEAVVEIGPVAANGFKTVPIRVDTGRRGLFSLQGQLDRPDA
                     EPVEIVYSVLVKPAGRTILGAHATISPTGLAALKRGGVSSVEALTDTLHRLSYLRPAP
                     DRFGWFDEKADWLEQSGLEGIGNLQYFNAEGDFSWAYDTPKKVDPPVAMRGRVVDEVT
                     YLKDDIWRDHVREVVGHYRKAYRRWVVDDEVSGGNTAFMYLEVLRSAREAARSVDPEI
                     KVSTSADGPWFEELIELGGRDVFDAVGGSVGGVPYWHALKLGRLKEMYNKENWVTGI"
     gene            complement(1518..2627)
                     /locus_tag="PKN80_04035"
     CDS             complement(1518..2627)
                     /locus_tag="PKN80_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35214.1"
                     /translation="MGSSGSRRLICFLFACLLLIIGAASFAAAGELPAGGLDARDLEG
                     LEPLVSRIPDQAGELAGLACAFHFRDQRIQQTVFRKVRRQNVWHNLLYWDRVTYRPES
                     GELCFDATLYQYTDRRKPLLIRLPFAVKVDETAGRAAVRAADRLTLEAVLSGRFQSAA
                     VNPDKSYGLALESPRVKLLAGERPLLAERAARLLRPEELPAAGSGLSSGAPIKETAVL
                     EMEAEAVEGLKFYADWEPDLDGWYAREITRGVSGKGLAVVSARNTGASFTLRLPQPLP
                     AGLYRVQVQPHYNSARFRDNILEVRLGRETARLGWFYVSDWPSTPVIEAAGPVSEVVI
                     KAVQVGGGGLNAAPEWPELVILLDKIRITRVEEKP"
     gene            complement(2681..4195)
                     /locus_tag="PKN80_04040"
     CDS             complement(2681..4195)
                     /locus_tag="PKN80_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HNQ35215.1"
                     /translation="MTGGQEPVLFDIRACGARPGPEHLNTGPIQRAIESAHQAGGGTV
                     YCPAGTFVTGTLRLKSRVNLHLEAGCTLLASPRREDYQDPSGVAESDAFGFENVSDAH
                     LIIGYQVEDAAITGFGSIDGNSRAFLPLSGPKWDRKAFAWRPGQLIYFCRSDRIRVEG
                     VSLLNAPYWTLFFHGCRDVRVNRLRIENPPETPNGDGIDIDCCRNILVSDCLVDSGDD
                     CLTIRAHARPLGAHALACENVVVRNCVLSSPCNAVRVGVGAGEIRNCLISGLVIQHSR
                     TGLNLFSCYGDFEPPGAAIRGLRFSDLVIEAEMPFMFTTGRRGQAPVEDLVLSNISAR
                     ASRASYFGGRSGNPIRGLTLENIIYTLDGGRETPPLSLAEFEWRREAESGLAAGGLPV
                     GLVFWDVEDLAVDRLRFRPGRGEGRWDHLIYGRNLDRIRLTGCELAGRFSPAPGAAIR
                     LADCRRTAVIGCAAGPEADPFLAVEGAGPPEPVTMIGNDLSRCRQPITGAPAAG"
     gene            complement(4220..6265)
                     /locus_tag="PKN80_04045"
     CDS             complement(4220..6265)
                     /locus_tag="PKN80_04045"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNQ35216.1"
                     /translation="MRILKVMAAGLLLAAGLAGYARAGELQWFMGNTHVHTTLGDGDS
                     PPETVVQWYHDHGYNFLVLSEHNFFIDPATVKLPENKRPDFILVPGEELTGKYRKIHA
                     GALNGGRLIRWDHETQVNSEFIQHLIDDAYDGMTVPILNHPNWRGGVSTADMLPVKGL
                     RLMEIFNGIPDTFSFGEGGRIPMEQKWDTLLTEGREVFGVASDDAHTFKTFKREAYNP
                     GRGWIFVRAGELSPSALTEAIYRGDFYASCGVALSEIVVDKGLIKIAIDEARTRKLAG
                     AGLGYGRRPAEGPEGFRLELIGEKGVLLEKRPGLRAEFKIPADQAYVRCRAVLTKKTD
                     DGFEELYAWGQPCFRADRQAANLKVQNEVELTRRQPVFDRVIRLEEGRSDYRLEMKVT
                     NPFDSVLNGSYEWNGLDRAWKVSPASGKLALGPSGSASVDFKVTYAGSESSAAILPEL
                     TVRWNLPGGGELSRKLLLPLDLQDYALKMKRNTAAAFRAGSAPVIDGRISETIWNRPP
                     DFKDFSYWLPFDIAIRPKDVTEGWAAYDQSALYLAFRCYDSDLAGLKTGNASSSGNDA
                     LEFLIDGDGDGRTYAWLFADAAGNKAANLRETGRKTGELPQPWNVAAGREEKAWTLEA
                     AIPWEALGIKAPAAGGSLKLEVARNLPRRFRSWGVVQWAPTFLNNHSPARFGTLKFQ"
     gene            complement(6320..>8455)
                     /locus_tag="PKN80_04050"
     CDS             complement(6320..>8455)
                     /locus_tag="PKN80_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35217.1"
                     /translation="YDHQEELVKRGEVNETLPASTTVRRKLPLADLPWGGYRLACSVE
                     GEPVLGDGLLALLPRIDQDAFPWFGADATLTQKDFMPRYMKRLGMKVAATCSCAGGIS
                     RWSIVNPAEGQYNWQDEVVDAAVEAGLAPVAYLGLKYPPAWVAAKYMKDGQVTDEAGF
                     IKAYTDYVYAFVKHFAGRIKVFQMEDEIGSAYGGAAKDSLLMRIHTAAWQAGQRAARE
                     TGQAVSIGIDSTQPDWWDKFLDLVDRNQLDYVSQNTNLRPAWKAQLLNTMRRKGAYPE
                     YLYSMGVGQKSVLRPTSLINVRGGDGNPPGVFAWQLMMNAWLSRPYGTEDPKDGPIVR
                     MNYYDFRTLGQSIYLPSAGKTGIEYDNSPTLGIQMMAMQKYWLSGLRPARDPKQEYTL
                     KGEPTGHERLFIYPFRDREKAVLVLTTVDASDVKAADFNCRWRLSGVDFKAYRPLDIY
                     GQPLKVAADGSAEVRSLPVLFTGLAASGLPGALAAFRELKAEALAGADHHRLEVGRYV
                     LEIDPERPGYLSLLTVDGKKETVILERIVGVPELPKPTVSVNASRVESSAEISFGEAG
                     KLDVNLNPKGVTFSWSRINRQTVPMSQKVRFRLSNEGAGREIVIQEGEKVTAGRLRED
                     YGELVPATAPAPIRLHPEASRVSLRDFAVFDLPGATGQGGFSPKSGFRFKLLGGDGVL
                     EADYTINGYPGGGARGTQSIKLEMRVSQP"
BASE COUNT         1390 a         2952 c         2706 g         1407 t
ORIGIN      
        1 gatgccggta acccagttct ccttgttgta catctccttg aggcggccga gcttgagggc
       61 gtgccagtag ggaaccccgc cgaccgagcc gccgaccgcg tcgaagacgt cccggccgcc
      121 cagctcgatc agctcctcga accaggggcc gtcggcgctg gtgctcacct tgatctccgg
      181 atcgaccgat cgggccgcct cccgggcgct gcgcagcact tccaggtaca tgaaggcggt
      241 gttgccgccg ctcacctcgt cgtccaccac ccagcgccgg taggccttcc ggtaatggcc
      301 gaccacctcc cgcacgtggt cgcgccagat gtcgtccttg aggtaggtca cctcgtccac
      361 cacccggccc cgcatggcaa ccggcggatc aaccttcttc ggggtgtcgt aggcccagct
      421 gaagtcccct tcggcattga agtactgcag gttgccgatc ccctccaggc cggactgctc
      481 gagccagtcg gccttctcgt cgaaccagcc gaaccggtcc ggggccggcc gcaggtaact
      541 gaggcggtgg agggtgtcgg tgagcgcctc caccgaactt accccgcccc gcttgagggc
      601 cgccaggccg gtgggactga tggtggcgtg ggcgcccagg atggtccggc cggccggctt
      661 gaccaggacc gaataaacaa tctcgaccgg ctcggcatcc ggccggtcca gctgcccctg
      721 caggctgaag agtccgcgcc ggccggtgtc aacccggatc gggaccgtct tgaatccgtt
      781 ggccgccacc gggccgattt ccaccaccgc ctccggcccc ttccggtcgt agatgtcggt
      841 gaccgtgtaa cggaagcggg gcgtttcctg gccggcggtg tggttggcca cccagagttc
      901 caccgcgccc gcctcgccgg gatagaagac cttggccggc acttcgcagg tcaggccggc
      961 ctcgagcggc cggcgcgggc tgaagggctg gggcgactcc ccggcggtca gcgcggccgc
     1021 gtccacccag aggtggccgc cggccgccgc ggacaacggg gtgatgcgga cattgacgat
     1081 ctccttcggc agcttgacct tgaggtcaaa acgctgccac tcggcggtca cgttgaagcg
     1141 cgcctggccg tacatggtgc cggactgaat ggcgccggcg gacttctcgc tctcggccat
     1201 caactgaacc tggaccggct ggctggcctt gagctggacc gaaaagacgt aatccccggc
     1261 ggccggaatt cggaccggac ccgagaagag cggccgcgcc agcaggggca gtttcagact
     1321 ggtgcggccg tggaccgcgg tcgaggtgtc caggttggcc cggccgatgg gaccacccgc
     1381 atccaggccg atgacctccc aggccagcgg cccggcgccg gcctcgaagc tggcgtcgcg
     1441 cagcaggttc tcggcggcgg ccgaaagcgg gatcaagacc gccagcaaga gtaaaccggc
     1501 cagtatgttt ttccgtttca tggcttctcc tcgacccggg tgatcctgat cttgtccagc
     1561 aggatgacca gttccggcca ttccggcgcg gcgttcaaac cgccgccgcc cacctggacc
     1621 gccttgatga ctacctcgga gaccggcccg gcggcctcga tgaccggggt ggaaggccag
     1681 tcgctcacgt agaaccagcc gaggcgggcc gtctcgcggc ccaggcgaac ctccaggata
     1741 ttatcccgaa aacgggcgct gttgtaatgg ggctggacct ggacccggta cagtccggcc
     1801 ggcaggggct ggggcaggcg gagcgtgaag gaggcgccgg tgttccgggc gctcaccacg
     1861 gccaggccct tgccgctgac cccgcgggtg atttcgcggg cgtaccagcc gtccaggtcc
     1921 ggttcccaat cggcgtagaa cttgagcccc tcgaccgctt cggcctccat ctccaggacg
     1981 gccgtctcct tgatcggggc gccgctggag aggccgctcc cggccgccgg cagctcctcc
     2041 ggacgcagga ggcgggccgc cctttcggcc agcaacggac gctcgccggc cagcagcttt
     2101 acccgcgggc tctccagcgc caggccgtag gatttgtccg gattgacggc ggccgactgg
     2161 aagcggccgg agagaaccgc ctccagagtg agccggtccg cggctcggac cgcggcccgg
     2221 ccggccgtct cgtcaacctt gaccgcgaag ggcaggcgga tgagcagtgg cttgcggcgg
     2281 tcggtatact ggtagagggt ggcgtcgaaa caaagctccc cggactccgg ccggtaggtc
     2341 acccggtccc agtagagcag gttgtgccag acgttctggc ggcgcacctt ccggaagacc
     2401 gtctgctgga tgcgctggtc ccggaaatga aaggcgcagg ccaggccggc cagttcgccg
     2461 gcctggtccg gaatacggct gaccagcggc tccagccctt ccaggtcgcg ggcatccagc
     2521 ccgccggccg gcagctcgcc ggcggcggcg aaggaggccg caccaatgat caataacagg
     2581 caggcgaata agaagcagat caagcggcgg gatcccgatg aacccatggg caacctcctt
     2641 ttagttacta ttgtacacct tgattccata aaaaaccatc tcagccggcc gccggcgcgc
     2701 cggtgatcgg ctgccggcag cgcgacaggt cgttgccgat catggtcacc ggctccggcg
     2761 ggccggcgcc ctccaccgcc aggaacgggt cggcctccgg gccggccgcg cagccgatga
     2821 cggccgtccg ccgacaatcc gccagccgga tcgcggcgcc cggcgccggg gagaaccggc
     2881 cggccagctc gcagccggtc agccggatcc gatccaggtt gcggccgtaa atgagatgat
     2941 cccagcgccc ctcgccgcgg cccggacgaa aacggagacg gtcaacggcc aggtcctcga
     3001 cgtcccaaaa gaccaggccg accggcagtc cgccggccgc caggccggac tccgcttccc
     3061 gtctccattc gaactcggcc agggagaggg gcggcgtctc ccggccgccg tccagcgtat
     3121 aaataatatt ctccagggtc agaccccgga tcgggttccc ggaccggccg ccgaagtaac
     3181 tggcccggct ggcccgggcg ctgatgttgc tcaaaaccag gtcctcgacc ggcgcctgcc
     3241 cccgccggcc ggtggtaaac atgaatggca tctccgcctc gatcaccagg tcggagaaac
     3301 gcaggccgcg gatggcggcc ccgggcggtt cgaaatcgcc gtagcaggaa aagaggttga
     3361 gcccggtccg gctgtgctgg atgaccagtc ccgagatgag gcaattgcgg atttcgccgg
     3421 cgccgacgcc cacccggacc gcgttgcagg gcgaactcaa gacgcagttg cgcaccacga
     3481 cattctcgca ggccagggcg tgggcgccga gcggccgggc gtgcgcccgg atggtcaggc
     3541 agtcgtcgcc ggagtcgacc aggcagtcgg agaccaggat gttccggcag cagtcgatgt
     3601 cgatcccgtc gccgttgggt gtctccggcg ggttttcgat ccggaggcgg ttgacccgga
     3661 catcccggca gccgtggaaa aagagagtcc aataaggcgc gttgagcagg gaaacgccct
     3721 ccacccggat ccggtcggaa cggcagaagt agatcagctg gccgggccgc caggcgaagg
     3781 ccttccgatc ccacttggga ccggataacg gcaggaaggc gcggctgttg ccgtcgatgc
     3841 tcccgaaacc ggtgatcgcc gcgtcctcca cctggtagcc gatgatgagg tgggcgtcgc
     3901 tgacgttctc gaaaccgaag gcgtcggatt cggccacccc ggatggatcc tgataatcct
     3961 cccggcgcgg gctggccagc agggtgcagc cggcctccag gtgcaggttg acccggctct
     4021 tcaaccggag cgtcccggtg acgaaggtcc cggccgggca gtagacggtg ccgccgccgg
     4081 cctggtgggc cgactcgatc gcccgctgga tgggcccggt attcaggtgc tccgggccag
     4141 gccgggcgcc gcaggcccgg atgtcaaaaa gaaccggctc ttgaccgccc gtcattttcc
     4201 cccttgatac cgccggaaac tactgaaatt tcagcgtccc gaaccgcgcc ggactgtggt
     4261 tgttcaggaa ggtcggcgcc cactggacca ctccccaact ccgaaaccgg cggggaaggt
     4321 tacgggcgac ctccagcttc aggctcccac cggccgccgg cgccttgatt cccaacgcct
     4381 cccagggaat ggccgcctcc agcgtccagg ccttctcctc ccggccggcg gcgacgttcc
     4441 agggttgggg cagttccccg gtcttgcggc cggtctccct caagttggcc gccttgttgc
     4501 ccgcggcatc cgcgaacagc caggcgtaag tgcggccgtc gccgtccccg tctatcagga
     4561 attccagcgc gtcgtttcca ctcgacgagg cgttgccggt cttcaggccg gccaggtcgg
     4621 aatcgtaaca tcggaaggcc aggtaaaggg ccgactggtc ataggccgcc cacccttcgg
     4681 tgacatcctt gggccggatc gcgatatcaa agggaagcca gtaagaaaaa tctttgaaat
     4741 ccggcggccg gttccagatc gtttcggaga ttcggccgtc aatcaccggc gcgctgccgg
     4801 ctctaaaggc cgcggccgtg ttccgcttca tcttcagggc gtaatcctgc aggtcgagtg
     4861 gcagcaaaag tttacggctc agttccccgc cgcccggcag gttccagcgc accgtcagtt
     4921 ccggcaggat ggcggcgctg ctctccgaac cggcataggt aaccttaaaa tccacgctcg
     4981 cggacccgga agggccgagc gctaacttac cggaggcggg cgataccttc caggcccggt
     5041 ccaggccgtt ccactcgtaa cttccgttca gcaccgaatc gaaagggttg gtcaccttca
     5101 tttccaaccg gtaatcggac cggccctcct ccagccggat aacccggtcg aagaccggct
     5161 gccgccgggt aagttccacc tcgttctgca ccttgaggtt ggccgcctgc cggtcggccc
     5221 ggaagcaggg ctgtccccag gcgtataact cctcgaaccc gtcatcggtc ttcttcgtca
     5281 ggaccgcccg gcagcggaca taggcctggt cggccggaat cttgaactcc gcgcggaggc
     5341 ccggccgctt ctccagcaaa acgcccttct ctccgatcag ctccagccgg aaaccttccg
     5401 gaccctcggc cggccgccgg ccgtagccca ggccggcccc ggccagtttg cgcgtccggg
     5461 cttcatcgat cgcgattttg atcaagccct tatccacgac gatctcgctc agcgccactc
     5521 cgcaggaagc gtaaaaatcg ccccggtaaa tcgcctcggt cagggccgac ggcgaaagtt
     5581 ctccggcccg aacgaagatc cagccccggc cggggttgta ggcctcgcgt ttgaaggtct
     5641 tgaaggtgtg ggcatcgtcg gaggccaccc cgaagacctc gcgtccctcg gtgagcaggg
     5701 tatcccattt ctgctccatg ggaatgcgcc cgccttcccc gaaactgaag gtgtccggga
     5761 tgccgttgaa tatctccatc agccggagtc ccttcaccgg cagcatgtcg gcggtgctga
     5821 ccccgccccg ccagttgggg tggttcagga tcgggaccgt catcccgtcg taggcgtcgt
     5881 cgatcaagtg ctggatgaac tccgagttca cctgggtttc gtggtcccag cggatcagcc
     5941 ggccgccgtt gagcgcgccg gcgtgtatct tgcggtactt gccggtcaat tcctcgccgg
     6001 gaaccaggat gaaatcgggc cgcttattct ccggcagctt gacggtggcc gggtcaatga
     6061 aaaagttatg ctcgctgagc accaggaaat tgtagccgtg gtcgtggtac cactggacga
     6121 ccgtctccgg gggcgaatcg ccatcgccca gcgtcgtgtg gacatgggta ttgcccatga
     6181 accattgaag ttccccggcc cgggcatacc cggccaggcc ggccgccagc agcaggccgg
     6241 cggccataac ctttaaaatt ctcatttcct cgctcctttc ctctccggta agcgccggcc
     6301 ggaatcggcc ggcccgttat cagggttgac tgacgcgcat ctccagcttg atgctctggg
     6361 taccgcgggc gccgccgccg ggatagccgt tgatggtgta gtcggcttcc agcaccccgt
     6421 ctccgcccag cagcttgaag cggaaaccgc tcttgggcga aaagcccccc tggccggtcg
     6481 cccccggcag gtcaaaaacg gcaaaatccc tgaggctcac ccggctcgcc tccgggtgca
     6541 gcctgatcgg cgccggcgct gtcgccggaa ccagctcccc gtaatcctcc cgcagccggc
     6601 cggccgtaac cttctcaccc tcctggatca cgatctcacg ccccgctccc tcgttcgaca
     6661 gccggaaacg caccttctgg ctcatcggta ccgtctgccg gttgatccgg ctccagctga
     6721 aggtcacgcc cttcggattg agattcacat cgagtttgcc ggcctcgccg aaggagatct
     6781 cggcgctcga ttcgacccgg ctggcattga ccgaaaccgt cggcttcggc agctccggca
     6841 ccccgacgat ccgctccagg atcaccgtct ccttcttccc gtccacggtc agcaggctca
     6901 ggtagcccgg ccgctccggg tcgatctcca gcacgtaccg gcccacctcc aggcgatggt
     6961 ggtcagcccc ggccagcgcc tcggccttca gctccctgaa ggccgccagc gcccccggca
     7021 gcccggacgc cgccaacccc gtgaagagca ccggcagcga acgcacctcg gcgctcccgt
     7081 cggccgccac cttcaagggc tgtccgtaaa tgtccagcgg ccggtaggcc ttgaagtcca
     7141 ccccgctcaa ccgccaacgg cagttaaagt cggccgcctt cacatcgctg gcatccaccg
     7201 tggtcaaaac cagcaccgcc ttctcccggt cccggaaggg atagataaag aggcgctcgt
     7261 ggccggtcgg ctcgcccttc agcgtgtact cctgcttcgg gtcccgggcc ggccgcaggc
     7321 cgctcagcca gtacttctgc atcgccatca tctggatccc cagcgtcggc gagttgtcgt
     7381 attcgatccc ggtcttcccc gccgagggca ggtaaatgct ctgccccagc gtccggaagt
     7441 catagtagtt catgcgcacg atcgggccgt ccttgggatc ctcggtgccg tagggacgcg
     7501 agagccaggc gttcatcatc agctgccagg cgaagacgcc cggcgggttg ccgtcgccgc
     7561 cccgcacgtt gatcagcgag gtcggccgca gcacgctctt ctggcccacc cccatgctgt
     7621 aaaggtactc cgggtaagcc cccttccggc gcatggtgtt gaggagctgg gccttccagg
     7681 ccggccgcag gttggtattc tggctcacgt aatccagctg attgcggtcg accaggtcga
     7741 gaaatttatc ccaccagtcg ggctgggtcg aatcgatgcc gatcgagacc gcctggccgg
     7801 tctcgcgggc cgcccgctgg cccgcctgcc aggcggccgt atggatgcgc atcagcaggg
     7861 aatccttggc cgcgccgccg taagcactgc cgatctcgtc ctccatctgg aagaccttga
     7921 tccggccggc gaaatgtttc acgaaggcgt agacgtaatc ggtgtaggcc ttgatgaagc
     7981 cggcctcgtc ggtcacctgg ccgtccttca tgtacttggc cgccacccag gccggcggat
     8041 acttcaggcc gaggtaggcg accggggcca gcccggcctc gacggcggcg tcgacgacct
     8101 cgtcctgcca gttgtactgc ccctcggccg gattgacgat ggaccagcgg gagatcccgc
     8161 cggcgcagct gcaggtcgcg gcgaccttca tgccaagccg cttcatgtac cggggcatga
     8221 aatccttctg ggtcagggtg gcatcggcgc cgaaccaggg gaaggcgtcc tggtcgatgc
     8281 gcggcagcag ggccagcagc ccgtccccca gcaccggctc accctccacc gagcaggcca
     8341 gccggtaccc accccagggc aggtcggcca gcggcagttt ccgccgcacc gtcgtcgaag
     8401 ccggcagcgt ctcgttcacc tcgccccgct tcaccagctc ctcctggtga tcgta
//
LOCUS       DAOQYN010000071         8323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9047_length_8323_cov_4.776246, whole genome shotgun sequence.
ACCESSION   DAOQYN010000071 DAOQYN010000000
VERSION     DAOQYN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9047_length_8323_cov_4.776246"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PKN80_04055"
     CDS             complement(<1..710)
                     /locus_tag="PKN80_04055"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNQ35218.1"
                     /translation="MLGITIGDPAGIGPEIVLEALARLQPARRPVFVFGDRFVLEATG
                     RPIPAWVKLVEPGRFRPADFQPGSPSKATGIAALSYLEKAIDLARAGRIRCLVTAPVS
                     KSTISRPGRDFSGHTEFLARAYGISNYGMLFVGGRTRLLLLTTHLPLARVSGLINRDL
                     VIQKIRLARGFILDHWGGPEPVIVCCGINPHAGEAGTLGKEEKEVLEPALDRLRREGL
                     KIIGPVAADTAWQVYRANR"
     gene            complement(753..1616)
                     /locus_tag="PKN80_04060"
     CDS             complement(753..1616)
                     /locus_tag="PKN80_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ35219.1"
                     /translation="MAKLFPRLDSRRRRIALAGLLTAGLVRLLSGPAPLTGEPAILAR
                     VGNEVVTASELVKYVRLGVDQPNALEKLVEEKLLASEAGRLGYRVDPKKVETEFNRLG
                     RQRFQKQSDFYAWLREQKIPVAEVKKRLERELLMGLFIQNEITARIEVSPMEIGAYLS
                     EQRERLLRESSEYRLSEINFPRLESIPKDLAGLDTGFEDLGWVPFRNLSAPVQAALGG
                     LKAGGFSTPVEISGAWVIFKVLEMRGPGPESETGLAIAARRIIAQRKSREAYQEIIKR
                     LRDRYGVTYYN"
     gene            complement(1616..2551)
                     /locus_tag="PKN80_04065"
     CDS             complement(1616..2551)
                     /locus_tag="PKN80_04065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ35220.1"
                     /translation="MKFNPKRILEIARTGLRLRTLKNHRKSILAGLAGLLGLALILIL
                     YSFARDHFFGAKNDPVLATVNGRQIRSSDFKEQMAALPDFYQPYVAANPNQFLQDMIT
                     KELIYQKARRSRYSRDRQFRKKIEELRKELLIQEYVRQAVMANPEIPEADMKEYYKRN
                     PMDFIQPASIHLHEILVPDRRTAETVVSRFRAGDDFEDLARNYSQAESKAGGGDLGFV
                     REGKLNPPLLEKSVFSLQPGQISEPFETGAGFYIYLAGQKISSRQLNYEESKPLLKKL
                     MASKYSEKNLESFLVQLRSRSRIQVKQENLNKLKF"
     gene            complement(2574..4634)
                     /locus_tag="PKN80_04070"
     CDS             complement(2574..4634)
                     /locus_tag="PKN80_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNQ35221.1"
                     /translation="MAPEQNQRGDVLILVIGLLALLLAITIAFLALGRTGFRISHYSL
                     HEVKAREMAETGMAEAVARLKSDAPEVDSQADNWSRDFAGDEVDNNGDGHNESRWIAV
                     RRQGRLVGRYAVLVEDESAAVNLNAAGNLSRSERHGCHQGWQTHEIDLGKLPGIGTGI
                     AAGICRTRYGRDGRPGGSGDDDGDQTILAGDGIDNDGDGLVDEPGEGNNEPDEFDPEA
                     PAGDDQPFRSPDQAKEIPGLGPEIYRAIRNLISVFARDWNRDRHNRERIPINRATPAE
                     LAAAFIACGFETDRAAQLAVNLVDYRDRDSRPTRLEGPDGKIRLGLEQTPYLNEVELA
                     PAGEWRQRGDSLEYLASRGEFIELFNPYDRPLSIGGWQIKGGGLPPVTIRANAIIPPR
                     GYYTVGDSRAVWIRFQLVAGIPVPVPVEVTSIRLPTNADQYHDFLLLSFAGTDMRLED
                     PTGHPIEITRIGFDFPGAPTRQKNDPRLRGETDWYPGPPSPGRTNHLFLPESGLETTR
                     LEWPEHFRVKNRPLATVGELGRLHRMEPWRTLDLTRPNRPDLTTQDIFTVAEPPGRPT
                     AGRLNINTATREVLGCLPYLDAAAVTAIIEGRPYGEIGSVAPRLAYRSRPGEDLEERY
                     RRISNLITVRSSIFRIRVIGQSVEDRNENGIIEEKEVETEKRLTAVFDRDRPGRRFLY
                     WKED"
     gene            complement(4624..5262)
                     /locus_tag="PKN80_04075"
     CDS             complement(4624..5262)
                     /locus_tag="PKN80_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35222.1"
                     /translation="MTKRPPGKKGLTLLEILVAAFLTLIIVGAGLLLLHGLNRFWVAG
                     ETAIASQTRARTLISFLERLLPAALPPEGTVGRRPELVGNETTLVFWSRAGEREDRTE
                     LARIALFYRPESGTLYAAEKRQSGGDWPAELRNSDRETGQPLVFGLTRLEFAYHDGRQ
                     FLPAWDSRTGTGESDRLPRAVRVRFRFSENRNRVAGDEFETLIELKNRTDGT"
     gene            complement(5325..5681)
                     /locus_tag="PKN80_04080"
     CDS             complement(5325..5681)
                     /locus_tag="PKN80_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35223.1"
                     /translation="MRKGYSLLLVVIALGVLAIGILSIVSLLPTEERLVRRTVFNARS
                     ATVAEREFNRIRTLYGTAADPWPPAELEGREGSDLRWKAAIRNEGELYRVTLNVHIKT
                     KGREESESFETSFIRR"
     gene            complement(5696..6169)
                     /locus_tag="PKN80_04085"
     CDS             complement(5696..6169)
                     /locus_tag="PKN80_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35224.1"
                     /translation="MTSATGKSEPGRGGISLLEIVVGLAVMAALLWGTLPHFSGRRRS
                     QRLEAAAQKIEGTVSLCRQTAISRRRPHEIVMETSDATLLIRDAVTLQPVGPPERLPE
                     GVVIGEITGSLRPMVFRPDGSLSGVSGSIRVRETASGAERRVVVYGLTGQTRTVP"
     gene            complement(6150..6581)
                     /locus_tag="PKN80_04090"
     CDS             complement(6150..6581)
                     /locus_tag="PKN80_04090"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNQ35225.1"
                     /translation="MKSGFSLIEILVVLAIASILATLGLPAALKARQRGLTGKVEATL
                     ASIEAALVLYETDFGDYPPGSGTAGRLVKLLSEPVESPRWKGPYFGYKKRDYREGELI
                     DPWGMPYRYHYPQSIHANTPFLLYSCGPDRTERTPDDLGNW"
     gene            complement(6594..7895)
                     /locus_tag="PKN80_04095"
     CDS             complement(6594..7895)
                     /locus_tag="PKN80_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ35226.1"
                     /translation="MIQTMGTYKYNAVDPNGRNVVGTIDAEGVQGAISLLKERGLFIS
                     EISENRSAARPARGRGKGTQNAPAGQETTVTVKKKGKGLNFGAPVIKTKSLAIVTRQL
                     STLLAAGLPLLRALRTIRDQQRGGPGIVLNDLAAEVEQGSLLSQAMAKHPKSFPRIYV
                     AMVRAGETSGALDTVLNRLASFAESDLKLRGRIKAAMAYPSVILVVAIGIVAFIMLKI
                     VPVFVAMFADFEAGDLPAMTRALIFLSTFLTNRAWLGILILVAMGVTYRVLYQIPKTR
                     YMIDQIKIKLPVFGPLIYKTIIARFARTMATLISSGVPILTSFKIVEDTVANKVISGA
                     IVKIHDEVREGAGITNPMIKSRAFPPMLTNMVGVGEETGALDSMLDKVADTYDEEIER
                     TVEALSSMIEPLLIVFMGGIVGFIVIALFLPLIKIAMSMGS"
     gene            complement(7892..>8323)
                     /locus_tag="PKN80_04100"
     CDS             complement(7892..>8323)
                     /locus_tag="PKN80_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HNQ35227.1"
                     /translation="PSAITRMIDMNVEPFLLASTIIGVVGQRLVRTLCPHCKEEYTPS
                     EVELAKVGITPDQLEGKVLYKARGCGRCKAGYRGRVAIFEILTPDEEFWSMVLARKTV
                     GELRRHAIKHGMRTMVTAGKDKILEGVTAIDEVAKEIHGYT"
BASE COUNT         1401 a         2500 c         2704 g         1718 t
ORIGIN      
        1 cgattggccc tgtaaacctg ccaggcggtg tcggcggcga ccgggccgat tattttcagc
       61 ccctcccggc gcagccggtc aagggccggt tccagaactt ctttttcttc ctttccgagg
      121 gtgccggcct caccggcgtg cgggttgata ccgcagcaga cgatgacggg ttccgggccg
      181 ccccagtggt cgagtatgaa accgcgggcc agccggattt tttggatgac caggtcccgg
      241 ttgatcaaac cgcttacccg ggcgagcggc aggtgggtgg tcagcaggag aagacgggtc
      301 cggcctccga cgaagagcat cccgtagttg gaaattccat aggcccgggc caggaattcg
      361 gtgtggccgg aaaaatcccg gccgggccgg ctgatagtcg acttggagac cggggcggtt
      421 accaggcagc ggatccggcc cgcacgggcc agatcgatcg ctttttccag gtaggacagc
      481 gccgcgattc cggtggcttt ggaaggggat cccggttgga agtcagccgg acggaagcgg
      541 ccgggttcaa ccaatttcac ccaggcggga atcggccggc cggtggcctc caggacgaag
      601 cggtctccga aaacgaaaac cgggcggcgg gccggttgca ggcgggccag ggcttcaagg
      661 acgatctccg gtccgattcc ggccgggtct ccgatggtta tgcccaacat ttatcaagcc
      721 ggtggttggt atcggcggaa cgtgcggttg gttcaattgt aataagtaac cccgtaccgg
      781 tcccggaggc gcttgattat ctcctggtaa gcctcccggc tcttgcgctg ggcaatgatc
      841 cggcgggccg cgatcgccag gccggtttcc gattccgggc cgggaccgcg catctccagc
      901 accttgaaga ttacccaggc gccgcttatc tccaccgggg tggagaagcc gcctgccttc
      961 aggccgccca gggccgcctg gaccggtgcg gataggtttc ggaagggaac ccagcccagg
     1021 tcctcaaatc cggtgtccaa cccggccagg tctttcggta tcgattccag gcgggggaag
     1081 ttaatttcag acaaccggta ttcgcttgac tcccggagca gccgttcgcg ctgctccgag
     1141 aggtaggcgc cgatctccat cgggctgacc tcgatccggg ccgtgatctc gttctggatg
     1201 aaaaggccca tcagcagctc tctttccaat ctttttttga cttcggccac tgggatcttc
     1261 tgttcgcgca gccaggcgta gaaatccgac tgtttctgga atcgctgccg gccgagacgg
     1321 ttgaattcgg tttccacctt tttgggatcc acccggtacc cgagccggcc cgcctcgctg
     1381 gccagcagtt tttcctcgac gagtttttcg agggcgttgg gttgatcaac cccgagccgg
     1441 acatacttga ccagttccga agcggtgacc acttcattgc ccacccgggc caggatggcc
     1501 ggttcgcccg tcagcggtgc cggtccggac agcagccgga ccagtccggc tgtcaacagg
     1561 ccggccaggg ctatccgccg gcgcctggaa tcgagccgcg gaaaaagttt ggccattaga
     1621 atttcagctt gttcaggttc tcctgcttga cctgtatccg ggagcggctg cgcagctgga
     1681 ccaggaacga ttccaggttc ttttcgctgt acttgctggc catcagtttc ttgaggaggg
     1741 gtttcgactc ctcgtagttc aattggcggc tcgatatctt ctgaccggcc aggtagatgt
     1801 agaaaccggc gccggtttca aagggttcgc tgatctggcc cggctggaga gaaaagacgc
     1861 tcttttccag gagtggcgga ttgagtttgc cctcccgaac gaaaccgaga tcgccgccgc
     1921 cggccttcga ttcggcctgg gaataattgc gggccaggtc ttcgaagtcg tcgccggccc
     1981 ggaaacggct tacgaccgtt tcggcggtgc ggcgatcggg gaccagtatt tcgtgcagat
     2041 ggatgctggc cggctggatg aagtccatcg ggttgcgttt gtagtactcc ttcatgtcgg
     2101 cctccgggat ctccgggttg gccatcaccg cctggcgcac atactcctgg atgagcagtt
     2161 cctttctcag ctcctcgatc tttttacgga actggcggtc ccgcgagtag cggctccggc
     2221 gcgccttctg gtaaataagt tccttggtga tcatgtcctg gagaaactga ttcgggttgg
     2281 ccgccacgta gggttggtag aaatcgggca gggccgccat ctgttccttg aagtcggaac
     2341 tccttatctg ccggccgttg acggtggcca ggaccgggtc gttcttcgcc ccgaagaagt
     2401 gatcccgggc aaaggaatac aggatcagga tcagggccag gccgagcagg ccggccaggc
     2461 cggccaggat gctctttcgg tgattcttca gcgtccggag cctgagtccg gtccgggcga
     2521 tttccaggat ccgtttcgga ttgaacttca tgctgaaact cctttcggtt cgatcagtct
     2581 tctttccagt aaagaaaccg gcgtcccggc cggtcacggt caaaaacggc ggtcagacgt
     2641 ttttccgtct cgacctcctt ctcttcaatt ataccatttt cattgcggtc ttcgacagat
     2701 tgaccgatca ccctgattcg gaagatcgaa gaccggaccg tgatcaggtt gctgatgcgc
     2761 cggtaacgtt cttcaaggtc ttcgccgggg cggctgcgat aggccaggcg cggggccact
     2821 gaaccgattt cgccgtaagg ccgcccctcg atgatggccg ttaccgcggc ggcgtcaagg
     2881 taaggaagac agcccagaac ttcccgggtg gcggtgttga tattaaggcg gccggccgtc
     2941 ggccgcccgg gcggttcggc cacggtaaag atgtcctggg tggtgagatc cggtcggttg
     3001 gggcgcgtca ggtccagggt gcgccagggt tccatccggt gcagccggcc caattcgccg
     3061 accgtggcca gcggacggtt tttcacccgg aagtgctccg gccattccag ccgggtggtt
     3121 tccagaccgc tttcgggcag gaaaaggtgg ttggtccgcc cgggggaggg ggggccgggg
     3181 taccaatcgg tttcaccgcg cagtcgggga tcattcttct ggcgggtcgg cgccccggga
     3241 aaatcgaaac cgatccgggt gatttcgatc gggtggccgg tcgggtcttc aaggcgcatg
     3301 tcggtgccgg cgaagcttag caggagaaag tcgtgatact gatcggcgtt ggtcggcagg
     3361 cgtatggaag tcacttccac cgggaccggg accgggattc cggccacgag ctggaaacgg
     3421 atccagacgg cccggctgtc cccgaccgtg taataacccc ggggcggtat tatcgcgttc
     3481 gcccggatcg taaccggcgg caggccgccg ccctttattt gccagccgcc aattgagagc
     3541 gggcggtcat acgggttgaa gagttcgatg aattcgcccc ggctggccag gtattcgagg
     3601 ctgtcgcccc gctggcgcca ctcgccggcc ggggccagtt ccacctcgtt caggtagggc
     3661 gtctgctcga ggcccagccg gatctttccg tccggcccct ccagccgggt tggacgcgaa
     3721 tcccggtcgc ggtaatcgac caggttgacg gccagttgag cggcccggtc cgtttcgaat
     3781 ccgcaagcga tgaaggccgc cgccagttcg gccggggtgg cccggttgat cgggatccgc
     3841 tcccggttat gccggtcccg gttccaatca cgggcaaata ccgagatcaa gttgcgaata
     3901 gcccggtaga tttccggacc caggccggga atctccttcg cctggtccgg cgatcggaag
     3961 ggttggtcat caccggccgg cgcttcgggg tcgaattcgt caggttcgtt atttccctcg
     4021 cccggttcgt caaccaaccc gtcgccatcg ttgtctatcc cgtcgccggc caggatcgtc
     4081 tggtcgccgt cgtcgtcgcc gcttccgccc ggccggccgt cccggccgta acgggtccgg
     4141 cagatcccgg ccgcgattcc ggttccaatc ccgggtagct tgcccaggtc gatttcgtgg
     4201 gtttgccagc cctggtgaca gccgtgccgc tcggaccggg agaggttgcc ggccgcgttc
     4261 aggttgacgg cggccgactc atcctcaacc aggaccgcgt aacggcccac cagccgaccc
     4321 tggcgtcgga ccgcgatcca gcggctttca ttgtggccgt cgccgttgtt gtccacttcg
     4381 tcgccggcga aatcacggct ccagttgtcg gcctggctgt ccacctccgg cgcgtcggat
     4441 ttgaggcggg ccacggcctc ggccatgccg gtttcggcca tctcccgggc cttgacttcg
     4501 tgcaggctgt aatggctgat ccggaaaccg gtccggccca gggccaggaa ggcgatagta
     4561 atggccagca ggagagccag cagcccgatc accaggatga gaacgtcgcc gcgttggttt
     4621 tgttcaggtg ccatcggtcc ggtttttcag ctcgatcagg gtttcgaatt cgtcgccggc
     4681 cactcggttc cgattttcgg agaagcggaa ccggacccgg accgcccggg gcaggcggtc
     4741 cgattctccg gtgccggtcc gggaatccca ggccggcaga aactggcggc cgtcatgata
     4801 ggcgaattcc agccgggtca gtccgaagac cagcggctgg ccggtttcac ggtccgagtt
     4861 tcgaagttcg gccggccagt cgccgccgga ctgccgtttc tcagcggcgt aaagggtgcc
     4921 ggattccggc cggtaaaaga gggcgatccg ggccagttcg gtacggtctt ccctttcccc
     4981 ggcccggctc cagaagacca gcgtggtttc atttccgacc agttccggac gccggcccac
     5041 ggttccctcc ggcggcagcg cggccggaag cagacgctcc aggaaggaga tcagcgtccg
     5101 ggccctggtc tggctggcga tggccgtttc gcccgccacc cagaaacggt tgagtccatg
     5161 gagcagcagg agcccggcac cgactatgat cagggtcagg aaggcggcta ccaggatttc
     5221 aagcagggtt aatccttttt tgccgggggg gcgcttagtc atgatggcga ttccggcggc
     5281 agagcatacc gatagttcgg acgcgcggcc ggtccgatcc ggttttagcg gcgaatgaag
     5341 ctggtttcga aggattccga ctcctcgcgt ccttttgttt tgatgtggac gttcagggtt
     5401 acccggtaga gttcgccctc gtttctgatg gccgccttcc agcggagatc gctgccttcc
     5461 cggccttcca attcggcggg cggccaggga tcggcggcgg ttccgtagag cgttcggatc
     5521 cggttgaatt cccgttcggc caccgtggcg gaccgggcgt tgaagacggt gcgccggacc
     5581 agcctttcct cggtgggcag cagtgagacg atggagagga tgccgatcgc cagaacgccg
     5641 agcgcaatga ccaccagcag gagcgagtat ccctttcgca tgcttctcga tgttttcagg
     5701 gaaccgtccg ggtctgaccg gtcagcccat agacaacgac ccgtctttcg gcgccgctgg
     5761 cagtttccct gacccggatc gagcccgaaa ccccgctcag gcttccgtcc ggcctgaaga
     5821 ccattggtct gaggctgccg gttatctccc cgatcaccac gccttccgga agcctttccg
     5881 gcggtccgac cggttgaagc gtaaccgcgt ccctgattaa aagtgtcgca tccgaagttt
     5941 ccatgactat ttcatggggc cggcggcggc tgatggccgt ctgccggcag agcgagacgg
     6001 ttccttctat tttctgggcg gccgcttcca ggcgctgtga acggcgccgg ccgctgaaat
     6061 ggggcagcgt tccccagagg agggcggcca tgacggccag ccccacgacg atttcgagca
     6121 ggcttattcc gccgcgtccc ggctcgcttt taccagttgc cgaggtcatc cggagttcgc
     6181 tcggtccggt ccggcccgca ggaataaaga aggaaagggg tgttggcatg gatggattgc
     6241 ggataatgat aacggtaggg catcccccag ggatcaatca actcgccttc ccggtaatcg
     6301 cgttttttgt agccgaagta ggggcctttc cagcgcgggc tttcgaccgg ctcggagagc
     6361 agtttcacca gccgcccggc ggtaccggaa ccgggcgggt agtctccgaa gtcggtttcg
     6421 tagagaacaa gcgcggcttc aatcgaagcc agggtggctt cgacttttcc ggtcaggccg
     6481 cgctgcctgg ctttaagcgc ggccggcagg ccgagggtgg ccaggatcga agcgatggcc
     6541 aggacgacca ggatttcaat cagggagaaa ccggatttca tcgccgggga tcatcagctg
     6601 cccatggaca tggcaatctt gatcaaggga aggaagaggg caatgacgat aaatccgacg
     6661 atacctccca tgaagacgat cagcagcggt tcgatcatcg atgacagggc ctcgaccgtt
     6721 ctttctatct cttcatcgta ggtgtcggcc accttgtcca gcatgctgtc cagggccccg
     6781 gtttcttctc cgacgccgac catgttggtg agcatcgggg gaaaggcccg gctcttgatc
     6841 atcgggttgg taatcccggc gccttcccgg acctcgtcgt ggatcttgac gatggcgccg
     6901 ctgatcacct tgttggcgac ggtgtcctcg acaatcttga aggaggtcag gatgggcacg
     6961 ccgctgctga tcagggtggc catggtccgg gcgaagcggg cgataatggt tttgtagatg
     7021 agcggaccga agaccggcag ctttatcttg atctggtcga tcatgtaacg ggttttgggt
     7081 atctggtaga gaacccggta ggtcacgccc atggcgacca gaatcagtat gccgagccag
     7141 gctcggttgg tcagaaaggt gctgagaaag atcagggccc gggtcatggc cggcaggtcg
     7201 ccggcttcaa aatccgcgaa catggccacg aagaccggca cgatcttcaa catgatgaag
     7261 gccacaatac cgatggccac caccaggatt accgacgggt aggccatggc cgccttgatt
     7321 cggccgcgca gcttgaggtc gctttcggcg aaggaggcca gccggttcag gacggtgtcc
     7381 agggcgccgc tggtctcgcc ggcccggacc atggccacgt agatgcgggg aaagctcttc
     7441 gggtgtttgg ccatcgcctg cgaaaggagg gaaccctgtt ccacttcggc ggccaggtcg
     7501 ttgagaacga tgcccggccc tccccgctgc tggtcacgga tggtccgcag cgccctcagg
     7561 agcggcaggc cggcggccag cagggtggac agctggcggg tgacaatggc cagtgatttg
     7621 gtcttgatga ccggggcgcc gaagttgagg ccttttcctt ttttcttgac cgtgacggtg
     7681 gtttcctggc cggccggagc gttctgggtg ccctttcccc ggccccgggc ggggcgggcg
     7741 gcgctccggt tttcactgat ttcggagatg aaaaggccgc gttccttcaa gagggaaata
     7801 gccccctgga ccccttcggc atcaatggtt ccgaccacgt tgcggccgtt cgggtcaacc
     7861 gcgttgtact tgtaggttcc catggtttgt atcaagtgta accgtgaatc tccttggcca
     7921 cttcatcgat cgcggtcact ccttcaagga tcttatcctt gccggcggtg accattgtcc
     7981 tcatgccgtg cttgatggcg tgccggcgca gttcgccgac cgtcttgcgg gccagtacca
     8041 tcgaccagaa ttcttcgtcc ggcgtcaaga tctcgaagat ggcgacccgg ccgcggtagc
     8101 cggccttgca gcgcccgcag ccccgggcct tatagaggac ttttccctcc agctggtcgg
     8161 gcgtaatgcc caccttggcc agttccacct cgctgggcgt gtactcttcc ttgcagtgag
     8221 ggcagagcgt gcgcaccagg cgctgtccta ccacgccgat gatggtggag gccagcagga
     8281 agggttcgac gttcatgtcg atcatgcggg ttatcgcgct ggg
//
LOCUS       DAOQYN010000072         8311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9066_length_8311_cov_4.023498, whole genome shotgun sequence.
ACCESSION   DAOQYN010000072 DAOQYN010000000
VERSION     DAOQYN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9066_length_8311_cov_4.023498"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKN80_04105"
     CDS             complement(<1..841)
                     /locus_tag="PKN80_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35228.1"
                     /translation="MHRRCLRFENLLLLGDGGIGRSFLHLAESELNGFRRVLVIDKAP
                     MRLPAGRKNAETVLADLSRSGVLGSFLVKFPYGSALVLNLTLGLDHLLLRRQAAGRGA
                     GYLDAAAGSLAAAPAENRLSRLAPYWLTPLKTEAPHWLGFGINPGLVELAARRLLEKR
                     AAGRRRLVVFEFDRLAARLDGDRVAVGWSPAGLIEEMMRMPAFFVEADRACESADNCS
                     GRVRVNWREKDLEVRRVAHEEIYNFSKSRRVAAAEFLYVLRPEVMRLLDGDPKGAERR
                     LIVP"
     gene            complement(1183..2502)
                     /locus_tag="PKN80_04110"
     CDS             complement(1183..2502)
                     /locus_tag="PKN80_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35229.1"
                     /translation="MDLFPRLSDARRLAAVRSATAVRHPAFGDLSRPGAVRLASGRRR
                     REAGYREADPLFIHLGVTGRCYARCKGCINAAVTHNSKEPRHRLTTQPDMEPERDGAA
                     VVRLAGLHPEMAVTVCFYGGEPLLVPERIARTAGILDASPIGGRVRYLLYTSGELIAA
                     ALERYPELMRRLWLYSVSIDGRTRQHDSVRSGTRLERIHRSLERLAEVRTGPVLQWST
                     LREEQSLLDCFREFLYLEGRGLASHFFWHWVETDEPYRDFPAYLARYERDLKRVLDVC
                     LDRLAAGRPLKIIHLDELLLYLFTGRRRGTTACGVEAAGNYDLGGGRVFACADLPPEL
                     AIGSVAADGSVELREAELARLAGYKERLGCPECGVEAYCGGRCPVEALTGTPERLFQY
                     CQLMRLHVGLVSARREEFRRLLKRRRISLQALYDRSAFMAAYTDVVP"
     gene            complement(2507..3550)
                     /locus_tag="PKN80_04115"
     CDS             complement(2507..3550)
                     /locus_tag="PKN80_04115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNQ35230.1"
                     /translation="MRRSWLLIGGAGCLAVLGLLGRGFFGRPEPDRPARISVAAVGDL
                     MPATDFPAGRLPPDDGRGLFDDAAGYLAAADLAFGNLEGPVCEGGACLKPLNRAGWHA
                     FRIPPRYAPRLAAAGFDVFSLANNHVRDFGPAGYEATRAALEKAGIGYADAEGQLAAF
                     RVRGRRVAVAAYYYGGGERSILRPEVPWSEIRRLDRDYDIVIVSAHGGAEGPAALRLP
                     FRPEYYLGEARGDLAAFGRGAVEAGADLVLMHGPHVPRALEVYRGRLIAYSLGNFCTT
                     GDFGLDGAAGLAPLLWVELDGRGRYLGHTVYSFRQAPGRGPKLDETDAAAELVDRLSR
                     LDFPGRPVSQTGD"
     gene            3658..6432
                     /locus_tag="PKN80_04120"
     CDS             3658..6432
                     /locus_tag="PKN80_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNQ35231.1"
                     /translation="MLQSILMIDTNLSPDGKKPGGSRLFLDLETTGLDPRTDAILEIG
                     ALRVESGIETAGLELLVRPGRPVPEFITRLTGIDDEMIRERGVDLAEALARLAPLLEG
                     AVVVGHNIDFDLRFLKTAGLAADGPALDTALLAALVRPAERAYSLSHLTRTFFDEREE
                     HRALADARHSWRLFQFLETEARGLEPEFLRKMAALLAPAEPRLAEFFLEAADAAPPEP
                     VRESGPAYGKARSGGRPAPETDPDRIAALLNEAGPFARVAGYEFRSQQAEMARLVARA
                     FRDKEFLAVEAPTGTGKSLAYLLAAVHFAVSRQAPVVISTNTRNLQGQIWEKEIPQVR
                     KALPGLAWKASRLLGQDNYFCWQSFHRIYAGAGNLNAADQAALAYLVSFSHLSREGLL
                     SESARFLQNRFPVLRYWMEDLKAGGDHCAGPRCSMAGRCFYQRARLAAQQSDIIITNH
                     ALTLSPPANFPVYRHLILDEAHNIEDAAAEAHSRGVESETVAGLLSRLAAGQRKKKEG
                     AALRLAAQETRAAWLSFSGLVNRMLPAGPPRALAALRNDPRWPEAEQRRSELLTRWQA
                     MEAELEGAGKRQGVIADPDFQAEENEERGLALRARRHREDLQFIFNPADNYVSYGGRA
                     ESEREGWFLKSTPVELDALLNRELFDAMESVVFTSATLTVGRKFDFFLRRWGVESRPG
                     LKAAQLASPFDFRRQALLAVPSNFTRFDYHGSEDLYVVALAEGIETVARTVGGKQLVL
                     FAARRRMEAAYYQVKENLAGAGFAVLCQNLDGSRSHLAGRLREGIGNYLLFGTRSFQE
                     GVDIPGLRAVLIEKMPFPGKQDPLLNARQQAILKRGGRPFQDYILPLAIISLRQAFGR
                     LIRNRQDRGVVVIFDPRPLEEYPEALASLPDCPRIIADTPRFYEKLKEAVNRLYERKP
                     EGVKKAGAAG"
     gene            6468..6989
                     /locus_tag="PKN80_04125"
     CDS             6468..6989
                     /locus_tag="PKN80_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNQ35232.1"
                     /translation="MEECLRIFHFIDYLPKLAGAVLILIFGWLLATLACRITRRLLEH
                     TPRTDPLVMRYLAGIVRAAFLGVALIAALSKVGIDVGPFLVGLGAGGLVIGFALRDIL
                     ADFANGTMLLIYRPFHIDDYVKVGGETGFVSEITIVKTRLRTPAGDEIFVPNKNVWSQ
                     VITRYREAPRPGA"
     gene            complement(6952..7587)
                     /locus_tag="PKN80_04130"
     CDS             complement(6952..7587)
                     /locus_tag="PKN80_04130"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HNQ35233.1"
                     /translation="MELVIATGNAKKLGEILAILAGLPLRVLSLADFPEVAEPAEDGL
                     TFEANARKKAAGYARGLGRPVLAEDSGLEVAALDGAPGIRSARFAGPGKRDADNIALL
                     LERLKEVAPADRRARFVSVVALAAPDGRTTVFRGVREGLITGAPRGSNGFGYDPVFLV
                     PELGRTFAELAAGEKNRLSHRRQALELAREFLASPAGREFMRRAGAPPGTG"
     gene            complement(7656..>8311)
                     /locus_tag="PKN80_04135"
     CDS             complement(7656..>8311)
                     /locus_tag="PKN80_04135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ35234.1"
                     /translation="RCAYCLVSRIRGPLVSRAPEQVRAEVDRLAGAGYREAVLCAVNL
                     GAYGLDRGSRLVELLRLLAEPAGRPEGFGLRLSSIEPDRLTVELLETLAAGRLFRRHL
                     HLPFQSGSDRILAAMGRRYRRGEYQRLVDLARRLIPGLFLSTDVMTGFPGESEADFEA
                     TLDLLRDISFNRVHIFRFSPRPGTPAAEWPDQVPEPVKSKRARRLKEQVRMEPVAAD"
BASE COUNT         1453 a         2902 c         2750 g         1206 t
ORIGIN      
        1 gcggaacaat caaccgccgt tcggccccct tcggatcgcc atccagcagg cgcatcactt
       61 ccggacgcag aacgtagagg aattcagccg ccgccacccg ccgggacttc gaaaaattgt
      121 aaatctcctc gtgggccacc cggcgtacct ccaggtcctt ttcccgccag ttcactctta
      181 cccggccgga acaattatcg gccgactcgc aggcgcggtc cgcttcgaca aaaaaagcgg
      241 gcatgcgcat catctcctcg atcaacccgg ccggcgacca gccgacggcg acccggtctc
      301 cgtccagacg ggcggccagc cggtcaaact cgaagaccac cagccggcgg cggccggcgg
      361 cgcgcttctc cagcagccgg cgggcggcca gttcaaccag gccgggattg atgccgaatc
      421 ccagccagtg cggtgcttcg gtcttcagcg gcgtcagcca gtaaggcgcg agccggctca
      481 ggcggttttc agcgggggcg gcggccaggg aaccggcggc agcgtccagg tagccggcgc
      541 cgcggccggc ggcctggcgt cgcagtaaca gatggtccag gcccagggtc aggttgagca
      601 ccagcgccga accataaggg aatttaacca ggaaagatcc gagaaccccg gaccgggaaa
      661 gatcggccag aaccgtttca gcattcttcc gtccggccgg caaccgcatc ggcgccttgt
      721 cgatgaccag cacccgtcgg aaaccgttca gttccgactc ggccagatgc aggaaggaac
      781 ggccaatgcc gccgtcgccc agcaacagaa ggttttcaaa acgaagacaa cggcggtgca
      841 tggtcacctc ccttgaatca ccggcaaaac ccggagtccg accggcctta atccagggcg
      901 aaaaaacaat tttccgacaa cgccatcgat tacctggctg cttctcgatt gtgaaacggc
      961 ttcttttaaa gagccgaaaa cgaaaccgcc tcgcattgta taacaaaaaa aaccggcgtg
     1021 actatcttaa catagtttgc aattaaagac taattttttc tgccggaaca gattggatcg
     1081 ccggggccgg aaagacggga taatccgttg ggccgggagg ggatcggaaa aaccggtgat
     1141 aacgcaacca accaaattgt tcccgttaaa aaaccttcat tatcaaggaa caacgtccgt
     1201 ataggccgcc ataaaggccg accggtcata aagcgcctgc aatgaaatgc gccgtcgttt
     1261 caggagccgg cggaactcct cccggcgggc gctgaccagg ccgacatgca ggcgcatcag
     1321 ctggcagtac tggaaaaggc gctcgggcgt gccggtcagg gcctcgaccg gacaccggcc
     1381 gccgcagtag gcctcgaccc cgcactccgg acagccgagc cgttccttgt agccggccag
     1441 ccgggccagt tcggcctcgc gcagttccac cgaaccgtcg gcggccaccg agccgatggc
     1501 cagctcgggc ggcaggtcgg cacaggcgaa gacccggccg ccgccgagat cgtaattccc
     1561 ggccgcctcc accccgcagg cggtggtgcc gcgccgccgg ccggtaaaga ggtaaagcag
     1621 gagttcgtcg agatggatga tcttcagcgg ccggccggcg gccagccgat ccaggcaaac
     1681 atccaggacc cgcttcaaat cgcgttcgta ccgggccagg taggccggga aatcccggta
     1741 gggctcgtcg gtctcgaccc agtgccagaa gaagtggctg gccagaccgc gcccttccag
     1801 gtaaagaaac tcccggaaac agtccagcag cgactgttcc tcccggagcg tcgaccattg
     1861 gagcaccgga ccggtccgca cctcggccag cctttccagg ctccggtgaa tgcgctccag
     1921 gcgggtgccg ctccggaccg aatcgtgctg gcgggtccgg ccgtcgatcg agaccgaata
     1981 gagccagagg cgccgcatga gttccgggta acgctccaac gcggcggcga tgagctcgcc
     2041 gctggtgtaa agcaggtagc ggacgcggcc gccgatcggc gaggcgtcga ggattccggc
     2101 ggtccgggcg atccgctccg ggaccagcag gggttcgccg ccgtagaagc agaccgtcac
     2161 cgccatctcc gggtgaagac cggccagccg gaccaccgcc gcgccatccc gttccggctc
     2221 catgtccggc tgggtggtca gccggtggcg cggttctttg gagttgtggg tgaccgcggc
     2281 gttgatgcag cccttgcagc gggcgtagca gcggccggtc acgcccaggt gaatgaagag
     2341 cggatcggcc tcgcggtaac cggcctcccg gcgtcggcga ccggaagcaa ggcgcaccgc
     2401 ccccggccgg gagagatccc cgaaggccgg gtgacggacc gccgtcgcgg accggacggc
     2461 cgccaggcgg cgggcatcgg aaagtcgagg aaataaatcc atgcgctcaa tctccggttt
     2521 gcgaaaccgg ccggcccggg aaatccaggc gcgacagccg gtcaaccagc tcggccgcgg
     2581 cgtcggtctc gtccagcttc ggaccgcggc caggggcctg gcggaaagaa taaaccgtgt
     2641 ggccaaggta tcggccccgg ccgtccagtt ccacccagag caggggcgcc agcccggccg
     2701 ccccgtccag gccgaaatcg ccggtggtgc agaagttgcc caggctgtaa gcgatcagcc
     2761 ggccccggta aacctccagg gcgcgcggca cgtgcggccc gtgcatcaga accagatcgg
     2821 cgcccgcctc gaccgcgccc cggccgaagg cggccaggtc tccgcgcgcc tcgcccaggt
     2881 agtactccgg tcggaaaggc aggcgcaggg ccgccggtcc ctcggcgccg ccgtgcgccg
     2941 agacgatcac gatgtcgtaa tcccggtcca gtctccggat ttcgctccag ggaacctccg
     3001 gccgcagtat cgaacgctcc ccgcccccgt agtaataggc ggccaccgcc acccgccggc
     3061 cccggacgcg gaaggcggcc agctgcccct cggcgtcggc gtaaccgatg ccggctttct
     3121 ccagggcggc ccgggtggcc tcgtacccgg ccggcccgaa atcgcggacg tggttgttgg
     3181 ccagggagaa aacgtcgaag ccggccgcgg ccagccgcgg cgcgtagcgg ggcggaatgc
     3241 ggaaggcgtg ccagccggcc cgattcaggg gcttgaggca ggcgccgcct tcgcagaccg
     3301 gaccctcgag gttgccgaag gccaggtcgg cggcggccag gtacccggcc gcgtcgtcga
     3361 agaggccgcg gccgtcatcc gggggcagcc ggccggccgg gaagtcggtg gccggcatca
     3421 ggtcgcccac ggccgcgacg ctgatccgcg ccggccgatc cggctccggc cggccgaaga
     3481 agccccggcc cagcaggccc aggaccgcca ggcagccagc cccgccgatc agcagccatg
     3541 atcttctcat gagaacggtc atcaagaaat gccggtcaac cggtattact tatcacccta
     3601 taggataaca ccgggggccg gcggccgcca aaacgggctg acgataaaag ccgccaggtg
     3661 ttacaatcta tcctgatgat cgacaccaac ctgtcccccg acggaaagaa gcccgggggc
     3721 tcccggctct tcctggacct ggagacgacc ggcctggacc cgcgaacgga cgccatcctg
     3781 gagatcggcg ctctgcgcgt cgaatccgga atcgagaccg ccggcctcga gctgctggtg
     3841 cggccgggac ggccggtgcc cgagttcatc acccgcctca ccggcatcga cgacgagatg
     3901 atccgggagc gcggcgtcga tctggccgag gccctggccc ggctggcgcc cctgctggaa
     3961 ggggcggtgg tggtcggcca caacatcgat ttcgacctgc gcttcctgaa gacggccggg
     4021 ctggccgccg acggtcccgc tctggatacc gcgctcctgg ccgccctggt ccggccggcc
     4081 gaaagggcct actccctcag ccacctgacc cgcaccttct tcgacgaacg ggaggagcac
     4141 cgggccctgg ccgatgcccg ccactcctgg cgcctcttcc aattcctgga aaccgaggcg
     4201 cggggactcg aaccggaatt cctcaggaaa atggcggcgc tcctggcccc ggccgaaccg
     4261 cggctggccg aattcttcct cgaagccgcc gacgcggcgc ccccggaacc ggtccgggag
     4321 agcggcccgg cttacggaaa ggcccgctcc ggcggccgac cggcccccga gaccgacccg
     4381 gaccggatcg cggccctgct gaacgaggcc ggccccttcg cccgggtcgc gggttacgag
     4441 ttccgctccc agcaggcgga gatggcccgg ctggtggcgc gggccttccg ggataaggag
     4501 ttcctggcgg tcgaggcgcc caccggaacc ggcaagtcgc tggcctacct gctggccgcc
     4561 gtccatttcg ccgtctcccg acaggcgccg gtggtcatct ccaccaacac ccgcaacctg
     4621 caggggcaga tctgggagaa ggagatcccg caggtccgga aagccctgcc gggcctggcc
     4681 tggaaggcca gccgcctgct ggggcaggat aattatttct gctggcagag cttccaccgt
     4741 atctacgccg gcgccggaaa cctgaacgcc gccgaccagg ccgccctggc ctacctggtc
     4801 tccttcagcc acctgagccg ggaggggctc ctttcggaat cggcccggtt cctccagaac
     4861 cggttcccgg tactgcgcta ctggatggag gatctcaagg ccggcggcga ccattgcgcc
     4921 ggtccgcgct gttcgatggc cggccgctgt ttttaccagc gggcccggct ggccgcccag
     4981 caatcggaca tcatcatcac caaccacgcc ctgacccttt cgccgccggc caactttccg
     5041 gtctaccggc acctgatcct ggacgaggcg cacaacatcg aggatgccgc cgccgaggcg
     5101 cacagccgcg gagtggagag cgagacggtg gccggcctgc tgagccggct ggccgccggc
     5161 cagcggaaga aaaaagaggg cgcggccctc cggctggccg cccaggagac ccgggccgcc
     5221 tggctgagtt tctccgggct ggtcaaccgg atgctgccgg ccgggccgcc ccgggcgctg
     5281 gcggcgctcc gcaacgatcc gcgctggccc gaggcggagc agcgccgctc cgaactgctg
     5341 acccgctggc aggcaatgga agcggaactg gagggagcgg gcaaacgcca gggcgtcatc
     5401 gccgacccgg acttccaggc ggaggaaaac gaagaacgcg gcctggcgct gcgggcccgg
     5461 cgccaccgcg aggacctcca gttcatcttc aacccggccg ataattacgt ctcctacggc
     5521 ggccgggccg agtccgaacg ggagggctgg ttcctgaaat caacgccggt ggaactggac
     5581 gcgctgctca accgggaact cttcgatgcg atggagagcg tggtcttcac ctcggccacg
     5641 ctcacggtgg gacgtaaatt cgatttcttc ctgcgccggt ggggagtcga atcccggccg
     5701 ggcctgaagg ccgcccagct ggcctcgccc ttcgatttcc ggcgccaggc tctgctggcg
     5761 gtaccgagca acttcacccg tttcgactac cacggcagcg aagatctcta cgtggttgcc
     5821 ctggccgagg gaatcgagac cgtggcccgg accgtgggcg gcaagcagct ggttctcttt
     5881 gcggcccggc ggcggatgga ggcggcctac taccaggtca aggagaacct ggccggcgcc
     5941 ggtttcgcgg ttctctgcca gaacctcgac ggttcccgaa gccacctggc cggacgcctg
     6001 cgcgagggaa tcggcaacta cctgctcttc ggaacccgca gtttccagga gggagtggac
     6061 attccgggcc tgcgcgcggt cctgattgaa aagatgccct tcccgggcaa gcaggacccg
     6121 ctgctcaacg cccgccagca ggcgatcctg aaacggggcg gccgcccttt ccaggactac
     6181 atcctgccgc tggcgatcat ctcgctgcgc caggccttcg gccggctgat ccgcaaccgg
     6241 caggaccggg gcgtggtggt catcttcgac ccgcgtcctc tggaggagta cccggaggcg
     6301 ctggcttccc tgccggactg cccccggatc atcgctgaca cgccgcgctt ctacgaaaag
     6361 ctgaaagagg ccgtgaaccg gctctacgaa cggaaaccgg agggcgtgaa gaaggccggc
     6421 gccgccgggt agccggagac cggacggccg atcgagagga ggaatcaatg gaggagtgcc
     6481 tgcggatctt tcacttcatc gactacctgc ccaagctggc gggcgccgtc ctgattctca
     6541 tcttcggctg gctgctggcc acgctggcct gccggatcac ccgccgcctg ctggagcaca
     6601 cgccccgcac tgatccgctg gtgatgcgct acctggccgg catcgtgcgg gccgccttcc
     6661 tgggggtggc gctgatcgcc gccctgagca aggtcgggat cgacgtcggc cccttcctgg
     6721 tcggtctggg cgccggcggc ctggtgatcg ggttcgcgct ccgcgacatc ctggccgatt
     6781 tcgccaacgg gaccatgctg ctcatctacc gcccttttca catcgatgat tacgtgaagg
     6841 tgggcgggga gaccggcttc gtctccgaga tcaccatcgt caagacccgg ctgcgcaccc
     6901 cggccggcga cgagatattc gtacccaaca agaacgtctg gagccaggtg atcacccggt
     6961 accgggaggc gccccggccc ggcgcatgaa ctcccggccg gccggcgaag cgagaaactc
     7021 cctggccagt tccagcgcct gccgccggtg gctgagccgg ttcttctccc cggctgccag
     7081 ttcggcgaag gtccggccca gctccggaac caggaagacc ggatcgtagc cgaaaccgtt
     7141 cgaaccgcgc ggcgccccgg ttatcaagcc ctcccgcacg ccccggaaga cagtcgttcg
     7201 cccgtcgggc gccgccaggg cgaccaccga gacgaagcgg gcccggcggt cggccggggc
     7261 cacttccttc agccgctcca ggagcagagc gatgttgtcg gcatcccgct tgcccggccc
     7321 ggcgaaacgg gctgagcgga tcccgggcgc gccgtcgagc gcggccacct ccagccccga
     7381 atcctcggcc agtaccggcc gccccaggcc ccgggcgtag ccggccgcct tcttgcgcgc
     7441 gttggcctca aaggtcagtc catcctcggc cggttcggcc acttccggaa aatcggccag
     7501 gctcagcacc cgtaagggca ggccggccaa gatggccagt atttcgccca gttttttggc
     7561 gttgccggtg gcgatgacca gttccatttt tttgcggccg gtcttcaggc tcaaattcaa
     7621 gcccgaccgg accctctcct gcctttaaag accggtcaat cggcggcgac cggttccatc
     7681 cggacctgct ccttgagccg gcgggcccgc ttcgacttga ccggttccgg tacctggtcc
     7741 ggccactcgg cggccggggt cccgggccgc ggcgagaaac ggaagatgtg gacccggttg
     7801 aaagagatat cccgcagcag gtccagggtg gcctcgaaat cggcctcgct ctcgcccggg
     7861 aagccggtca tgacgtcggt ggaaaggaag agcccgggaa tcaggcgccg ggccaggtcc
     7921 accagccgct ggtattcgcc ccgccggtac cggcgtccca tcgccgccag gatgcggtcc
     7981 gaaccggact ggaacggcag gtgcaggtgg cggcgaaata accggccggc ggccagcgtt
     8041 tcaagcagtt ccaccgtaag gcggtccggc tcgatggagc tgagccggag gccgaaacct
     8101 tcgggccggc cggccggttc cgcgagcagc cggagcagtt cgaccaggcg gcttccgcgg
     8161 tccagcccgt aagcacccag gttgacggcg cagaggaccg cctcccggta gccggccccg
     8221 gccaggcggt caacttcggc ccgcacctgc tccggcgccc ggctgaccag cggcccccgg
     8281 atccgggaga cgaggcagta ggcgcagcgg c
//
LOCUS       DAOQYN010000073         8262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9141_length_8262_cov_5.207262, whole genome shotgun sequence.
ACCESSION   DAOQYN010000073 DAOQYN010000000
VERSION     DAOQYN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9141_length_8262_cov_5.207262"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PKN80_04140"
     CDS             <1..1203
                     /locus_tag="PKN80_04140"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ35235.1"
                     /translation="SEEAREVMDVLESGYLFRYGDPRNPKFKAKVWKFEQELAAYTGA
                     RHALAVNSGTSALLTGLAALGVGPGDEVIVPGYTFIASISSIIYARAIPVLCEVDESL
                     TMDPADVEARITPRTRAILPVHMLGNPCRMDRLMELARKHKLLVLEDAAQAAGASFQG
                     RKVGAIGDMGIFSFNVFKTITAGDGGAVITSDDNYYARAFGFHDQGHLPNRQGVEVGQ
                     RPFIGLDFRMTELSGAVLLAQLRKIDRIIGSLRIKKARFKAAISGCGDFSFRRINDPE
                     GECATLLTVIFPTREKARAVAERLKTTTVDHSGWHVYNNMEQILNQLTVTQERCPFTC
                     AYYKGQAEYRKGMLPRTDDILSRSMNISIGVSDAGLGAGFGITAVSGEDEIDRKAAEF
                     VRVVREVG"
     gene            1232..2149
                     /locus_tag="PKN80_04145"
     CDS             1232..2149
                     /locus_tag="PKN80_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35236.1"
                     /translation="MKTGWRGYPNCLKLTNRSSEVILAPEAGGRVLVFSREGRNILYQ
                     DRSVDGLTWQEGGKSFHADGGRFDVGPAWLIPPHLNLWLGPWSGTVTGPRSARLVSLY
                     DPATGLQLIRDFELDASESRLIIRQTMLNRSDRTIRWCYWGRTLADGAGCCVVPLNPE
                     SRFPRGFCMYGPGPYRLDYRPEGLRNIEVRDGFLIFTGGPEIPQYGFDTRAGWLAFLN
                     HGLLFVTRFPVYPERVYNEAGGFSAEVYYFQDRFCELEPVGPQEVLAPGASARFTEEW
                     WLFACPPPGPAFDPVELAGFVERNTEKVC"
     gene            2190..3311
                     /locus_tag="PKN80_04150"
     CDS             2190..3311
                     /locus_tag="PKN80_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35237.1"
                     /translation="MSQEKKGMGVAIIGCGGFVRGNHLPNAHRNPNLHLRALCDLDQP
                     LLERLAGQYHPDYVTTDHRKLAADPEIDLVIIGTTPEVRVELIKAAAEGGKNIYVEKP
                     MSLGYEDSAEIVQIIRRTGVKLQVGFNRPYSPIMQEAKRVFRKMRRQPTLIVYRIVGE
                     DLLRPMHHRTLLSSNAGSNIIHEITHIFNLLNWLTDQDPLTVYTAGDPSDDNIITLTY
                     PDRTYATIVAGNCGTAAYPKERMEVFSDFKALVMNEFVELECTRIPGESDQRFPLASN
                     PGRKVEGITVPELRQDLIAWHENISEAELRDGHYYNSVPRVNKGHYEALQDLYEAIRD
                     DRPVQVDAHAGAVATILGLEALESLKKNQPVKIDYSFLK"
     gene            3308..4246
                     /locus_tag="PKN80_04155"
     CDS             3308..4246
                     /locus_tag="PKN80_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35238.1"
                     /translation="MKTGRRGAVDIARVSRRGYPGCLRLAAGPVEVVLAPAAGGRVLS
                     YARNGTNILYQNPDLDGRLWAPGDPVFDPDGGRFDLGPEWVIPAHPRLWLGAWEAEVS
                     GPGAVRLTSPVEPDLGVQLVRDFYLAGDGSRLQFRQTVINRSELTRRWCYWGRTLAAG
                     GGVFLIPVNPESRFPKGFYLYERGHTLNVRPEKLRNVEVRDGYLVISGPPELPKYGLD
                     SRAGWLAYWRQGLLFTKRFPVYPNRVYNEAGGFTVEVYYFQDRFCELEPVGPEEILPP
                     GGRASFREEWRLLDWPENPLEKGPAEIGAFLEGYGR"
     gene            4259..5200
                     /locus_tag="PKN80_04160"
     CDS             4259..5200
                     /locus_tag="PKN80_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNQ35239.1"
                     /translation="MRAQPRVTGIGNCALDLLAGLDRYPGPDERVTARRILEQGGGEA
                     ATAMVTLARLGISTAFIGKIGDDWAGAAIRQGLEAEGVDASGLVVEPGRRSLFSFIAV
                     ENATGRRTIFSGRTTTRLKPSELARDFPGSSEVVHLDQRETRAGPRAAARARRAGARV
                     TLDVDRFEPATLKLVRATQVVLASGDFGRQAGPDPLAAAARVRELGPETVVFTYGDRG
                     ALVSGREGAFFQPAFRVRPVDTTGAGDVFRGAFIYGLLQEWPLERTARFASAAAALKC
                     TRPGGRTGIPGLERVILFLEERRNQARPRPLRPGDPG"
     gene            5234..5833
                     /locus_tag="PKN80_04165"
     CDS             5234..5833
                     /locus_tag="PKN80_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNQ35240.1"
                     /translation="MLLSIINIIGLVAFAFSGSVKGFSRRMDLFGVFLLGVTTALGGG
                     VVRDLLVNRMPVMLTDPAYLVFSLLGALLALAFRRRGTVIVNRWIFLVSDALGLAFFT
                     AAGCLVAREYGLGLVGILMLGTLTAVGGGMIRDVLAGEVPMVLRREVYASCSLLGGLG
                     FWLAGLAGSGPFLASLACMLPALALRLLAIRGHWQIPRP"
     gene            5862..7349
                     /locus_tag="PKN80_04170"
     CDS             5862..7349
                     /locus_tag="PKN80_04170"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNQ35241.1"
                     /translation="MVSQNREAPRSYRNPVRPGDFPDPSVVRVGEDYYLANSTFQYFP
                     GIILSHSRDLVNWRTIGHVLTRNSQLDLGPSADSAGIWAPDISWHDGRFWVAYTLQTP
                     TAGLNCLVWAERPEGPYSEPVILNRKSIDPSIFNDDDGRRYLATAYGTLQELAADGTR
                     LLGEPRQVWPGTGNRCPEGPHLVKVRGYYYFMMAEGGTGYDHTEVLARSRAIWGPYEP
                     CPYNPVLKPAGPGHPIQKTGHAKLVLTQRGEWWCVYLGGRPYGGKFCSLGRETFLGRV
                     NWTDDDWFVIEPPALEAERPDLPWSPAPDFSGNEFGDAGAMLQWETVRNPGPGQCLFK
                     PKKGLVALANQVLARRPEGFNFEALAVISRPLRSPRTRAGLVVYGDTVHNLQLGPAGG
                     RLELVRHRDRDREILAALPWSGTAWLRVRTSGQEYSFQASVDGERWQPVGPPLDGRFL
                     APESIMTGSLKRKCFTGLRIGIFNESDPAETTEFSRFEYRAAAGG"
     gene            7394..>8262
                     /locus_tag="PKN80_04175"
     CDS             7394..>8262
                     /locus_tag="PKN80_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35242.1"
                     /translation="MWKNKLKITAAALLMAAELAAGGLAGADEPGLLFGPTVTLRQNA
                     DGVRTLEDLKAVDGNFLLAFQQDLGENFMKLCEAAGVTVYLNSVGRPALRPGADPGDY
                     YQHSIFGAFGRDWREFLLRNAGRQDLHGLCCVPDEFTWHNAQLQYTLSIRPPRDWPFY
                     PSDETIGRRFKQETGLELPRFGHSRVLDGRDPAQRSYAAIRYRWTAEVLKEWSDQIRS
                     VNPRLKVGAVLTTLPAYGLERYPSGIAWDRLGESGALDLAVVTAFGTGHDYRGLDTHY
                     LISETAATLAAAFG"
BASE COUNT         1334 a         2744 c         2782 g         1402 t
ORIGIN      
        1 tcggaggagg ctcgggaggt aatggatgtt cttgagagcg gctacctgtt ccggtacggc
       61 gatcccagga acccgaagtt caaggccaag gtctggaagt tcgagcagga gctggccgcc
      121 tacaccggcg cccgccacgc cctggcggtc aacagcggca cctcggccct gctgaccggg
      181 ctggccgcgc tgggcgtggg gccgggcgat gaagtgatcg tgcccgggta caccttcatt
      241 gcttccattt cgtccatcat ttacgcccgg gccatcccgg tgctctgcga ggtggacgag
      301 tcgctcacca tggacccggc tgatgtcgag gccaggatca ctccgcgcac tcgggccata
      361 ctgccggttc acatgctggg caatccctgc cggatggacc ggctgatgga gctggccagg
      421 aagcataagc tgctggtcct tgaggatgcc gcccaggcgg ccggggccag tttccagggc
      481 aggaaggtcg gagccatcgg ggacatgggc atcttctcct tcaacgtctt caagacgatc
      541 accgccggcg acggcggtgc ggtgatcacc agcgacgaca attactacgc ccgggccttc
      601 ggtttccacg accaggggca cctgcccaac cggcagggag tcgaagtcgg ccagagaccc
      661 tttatcgggc tggacttccg gatgaccgag ctgagcgggg cggtcctgct ggcccagctg
      721 cggaagatcg accggatcat cggttcgctg cgcatcaaga aggcccgctt caaggcggcc
      781 atctccggct gcggcgattt cagcttccgc cggatcaacg atccggaggg cgagtgcgcc
      841 accctgctga cggtcatctt cccgacccgg gagaaggccc gggccgtggc cgaacggctc
      901 aagaccacca ccgtcgatca ctcgggctgg cacgtttaca acaacatgga acagatactc
      961 aaccagctga ccgtgaccca ggagcggtgc ccgtttacct gcgcctacta caaggggcag
     1021 gccgaatacc gcaaggggat gctgccccgg accgacgaca ttctgagtcg gtcgatgaac
     1081 atcagcatcg gggtctcgga tgccggcctg ggggccgggt tcgggatcac cgccgtttcc
     1141 ggggaggacg agattgatcg caaagcggct gaattcgtgc gggtggtccg ggaggtcggg
     1201 tgagcgaagc cggccggcgg cgggggcgcc agtgaagacc gggtggcggg gataccccaa
     1261 ctgcctgaag ctgaccaacc ggtccagcga ggttatcctg gccccggagg ccgggggccg
     1321 ggtcctggtc ttcagccggg agggccggaa tattctctac caggaccgga gcgtggacgg
     1381 cctcacctgg caggagggcg gcaagagctt tcacgccgac gggggccggt tcgacgtcgg
     1441 accggcctgg cttattccgc cccacctcaa cctctggctc ggtccctgga gcggaaccgt
     1501 gaccggtccg cgttcggccc ggctggtcag cctttacgac ccggccaccg gtctccagct
     1561 tatccgggac ttcgagctgg atgcctccga aagccggctg atcatccggc agacgatgct
     1621 taaccgttcc gaccggacca tccgctggtg ctactggggg cgcactctgg ccgatggggc
     1681 cggttgctgc gtggtgccgc tcaacccgga gagccgtttc ccgcgcggtt tctgcatgta
     1741 cggccccggt ccttaccggc tcgactaccg cccggagggg ctccggaaca tcgaggttcg
     1801 ggacggcttc ctgatcttta ccggcgggcc ggagattccc cagtacggtt ttgacacccg
     1861 ggccggctgg ctggcctttc taaaccacgg cctgctcttc gtcacccgct tcccggttta
     1921 cccggagcgg gtctacaacg aggccggcgg tttcagcgcc gaagtttatt acttccagga
     1981 ccggttctgc gaactggagc cggtcggtcc ccaggaggta ctggcaccgg gggcgagcgc
     2041 ccggtttacc gaggaatggt ggctctttgc ctgcccgccg cccggaccgg ctttcgaccc
     2101 ggttgaactg gccgggtttg ttgaacggaa cacggagaag gtctgctgac aagacacacg
     2161 ttgaaaaagg acaaccggaa ggagagggta tgagccagga aaagaagggc atgggggtgg
     2221 cgatcatcgg ctgcggcggt ttcgtccgcg gcaaccacct tcccaacgcg caccggaacc
     2281 cgaatctgca cctgcgggcg ctctgcgacc tggaccagcc gttgctggag cggctggccg
     2341 gccagtacca tcccgattac gtgaccaccg accaccgaaa gctggccgcc gatccggaga
     2401 tcgacctggt catcatcggg acgacacccg aggtgcgggt ggagctgatc aaggcggccg
     2461 ccgaaggcgg caagaatatc tacgtcgaga agccgatgag cctgggctac gaggattcgg
     2521 ccgagattgt gcagattatc cggcggaccg gcgtgaagct ccaggtcggc ttcaaccggc
     2581 cctactcgcc catcatgcag gaggccaagc gggtcttcag aaagatgcgc cggcagccga
     2641 ccctgatcgt ttaccggatc gtcggggagg acctgctgcg gccgatgcac caccgcaccc
     2701 tgctgtccag caacgccggc tccaacatca tccacgagat cacccacatc ttcaacctgc
     2761 ttaactggct gaccgaccag gatccgctta ccgtctacac cgccggcgat ccaagtgacg
     2821 acaacatcat caccctgacc tatccggacc ggacttacgc caccatcgtg gccgggaact
     2881 gcgggacggc cgcctacccg aaggagcgga tggaggtttt ctccgacttc aaggccctgg
     2941 tgatgaacga attcgtggag ctggaatgca cccgcatccc gggtgaatcc gaccagcgtt
     3001 ttcccctggc ttccaacccg ggccgaaagg ttgaggggat aacggttccg gaactccggc
     3061 aggacctgat cgcctggcac gagaatatca gcgaggcgga actgcgggac ggccactact
     3121 acaattcggt tccccgggtc aacaaggggc actacgaggc gctccaggac ctctacgagg
     3181 cgatccggga tgatcggccg gtccaggttg acgctcacgc cggcgcggtg gccaccatcc
     3241 tgggcctcga ggcgctcgaa tcgctcaaga agaatcagcc ggtaaagatt gactattcct
     3301 tcctgaaatg aaaacgggcc ggaggggcgc ggttgacatt gcccgggtga gccggcgcgg
     3361 ctatcccggc tgtctgcgcc tggccgccgg cccggtcgag gtcgtgttgg cgccggccgc
     3421 cggcggccgg gtcctctcct atgcccggaa cgggacaaac atactctacc agaatcccga
     3481 tcttgacggc cggctctggg cacccgggga tccggtcttc gatccggacg gcggccgttt
     3541 cgacctggga ccggaatggg tcattccggc ccacccccgg ttatggctcg gggcctggga
     3601 ggcggaggtg tccggtccgg gtgcggtccg gcttaccagc ccggtcgagc cggatctcgg
     3661 ggtccagctg gtgcgcgatt tctacctggc gggcgacggt agccgcctcc agttccgtca
     3721 gaccgtgatc aatcgttcgg agcttacccg gcgctggtgc tactggggcc gcaccctggc
     3781 cgccggcggc ggggtcttcc tgatcccggt caacccggaa agccggtttc cgaagggctt
     3841 ctacctctat gaacgcggcc acactctgaa tgtccggccg gagaagctgc ggaatgtcga
     3901 ggtgcgcgac gggtacctgg ttatcagcgg tccgccggaa ctgcccaagt acggcctgga
     3961 ttcccgggcc ggctggctgg cctactggcg gcaggggttg cttttcacaa agcgtttccc
     4021 ggtttacccg aaccgggtct acaacgaggc cggcggcttc acggtcgagg tctactattt
     4081 ccaggatcgc ttctgcgaac tggagccggt cggccccgag gagatcctgc cgccgggtgg
     4141 ccgggcctcc ttccgggagg agtggcggct gctggactgg ccggagaacc cgctggaaaa
     4201 agggccggct gaaataggcg ccttcctgga agggtacggc cgataagaag gggggctttt
     4261 gcgcgcgcaa ccccgggtga ccgggatcgg taattgcgcc ctggacctgc tggccggcct
     4321 ggaccgctac ccgggtccgg acgagcgggt taccgcccgc cggatactgg agcagggcgg
     4381 cggcgaggcg gcgaccgcca tggttaccct ggcccggctc ggcatttcaa ccgccttcat
     4441 cggcaagatc ggggacgact gggccggcgc ggccatccgg caggggctgg aggccgaagg
     4501 agtcgatgcc agcggcctgg tcgtcgaacc ggggcgccgt tccctctttt catttatcgc
     4561 ggttgaaaac gccaccgggc gccggaccat attctcgggc cggaccacca cccggctgaa
     4621 gccatcggaa ctggctcggg atttccccgg atcaagcgag gtagtccacc tggaccagcg
     4681 ggagacccgg gccggtcccc gggcggccgc ccgggcccgc cgggccggcg cccgggttac
     4741 ccttgacgtg gaccggttcg aaccggctac ccttaagctg gtccgcgcca cccaggtggt
     4801 cctggcctcc ggcgatttcg gccggcaggc cggccccgac ccgctggcgg ccgccgcccg
     4861 ggtccgggag ctggggccgg agacggtggt cttcacctac ggagatcggg gcgccctggt
     4921 cagcggccgg gagggagcct tcttccagcc ggccttccgg gttcggccgg tagataccac
     4981 cggcgccgga gacgtcttcc ggggagcctt catctacggt ctgctccagg aatggcccct
     5041 ggagcggacg gcccggttcg ccagcgccgc cgcggcgctc aagtgcaccc ggccgggcgg
     5101 ccgcaccggc atccccggcc ttgaacgggt gatccttttc ctggaagaac gtcggaacca
     5161 ggcccgaccc cggccgctcc ggccgggcga tcccggttga agcgggttcg gggtccaaac
     5221 ctgacgagcg cctatgctgc tctccattat caacatcatc gggctggtcg ccttcgcctt
     5281 ctccgggtcg gtcaagggct tcagccgccg gatggacctg ttcggagtct tcctgctcgg
     5341 ggtgaccacc gccctgggcg gcggcgtggt gcgggacctg ctggtcaacc ggatgccggt
     5401 gatgctgacc gacccggctt acctggtctt ctccctgctg ggcgcgctgc tggccctggc
     5461 cttccgccgg cgcggcaccg ttatcgtcaa ccgctggata ttcctggttt ccgacgcgct
     5521 gggcctggcc ttcttcaccg ccgccggctg cctggtggcc cgcgagtacg gcctgggcct
     5581 ggtcgggata ctcatgctgg gcacgctgac cgcggtgggc ggcggcatga tccgggacgt
     5641 gctggccggc gaggtgccga tggtcctgcg ccgggaggtt tacgccagct gctccctgct
     5701 gggcggcctg ggcttctggc tggccggcct ggccggctcc ggtcccttct tagcctcgct
     5761 ggcctgcatg ctgccggcgc tggcgctgcg cctgctggcc atccgcggcc actggcagat
     5821 tccccgtccg tgaaagatcc ggccgggcaa ggaggcaggt tatggtgagc cagaaccggg
     5881 aggcaccccg ttcgtaccgc aatccggtcc gccccggcga cttccccgac ccttcggtgg
     5941 tgcgggtggg cgaggactat tacctggcca actccacctt ccagtacttc ccgggcatca
     6001 ttctttccca ctcccgcgac ctggtcaact ggcgaaccat cggccatgtc ctgacccgga
     6061 actcccagct cgacctggga ccatcggccg attcggccgg gatctgggcg cccgacatct
     6121 cctggcacga cggccgcttc tgggtggcct acaccctcca gaccccgacg gccggcctca
     6181 actgcctggt ctgggccgaa cggccggagg ggccatactc ggagccggtc atcctgaacc
     6241 ggaagtcgat tgatccaagc atcttcaatg acgatgacgg ccggcgctac ctggccaccg
     6301 cctatggaac gctccaggag ctggccgccg acgggacgcg cctgctgggg gaaccgcgcc
     6361 aggtctggcc gggcaccggc aaccgctgcc cggaggggcc gcacctggtc aaggtgcgcg
     6421 gttactacta tttcatgatg gccgagggcg gcaccggtta cgaccacacc gaggtgctgg
     6481 cccgcagccg cgccatctgg ggcccttacg agccctgtcc ctacaacccg gtcctgaaac
     6541 cggccggtcc cggacacccg attcagaaga ccggccacgc caagctggtc cttacccagc
     6601 ggggcgagtg gtggtgcgtt tacctgggcg ggcggccgta cgggggtaaa ttttgcagcc
     6661 tgggtcggga gaccttcctg ggccgggtca actggaccga tgacgattgg ttcgtaatcg
     6721 aaccgccggc cctggaagcg gagcggcctg acctgccctg gtcgccggcg ccggacttct
     6781 ccggaaacga gttcggcgac gccggggcga tgctccagtg ggaaacggtc cgaaaccccg
     6841 gcccgggcca atgccttttc aagccgaaga agggcttggt ggcgctggcc aaccaggttt
     6901 tggcccgccg gccggaaggt tttaatttcg aggccctggc cgttatcagc cgacccttga
     6961 gatcgccccg gacccgggcc ggcctggtgg tctatggcga taccgtccac aacctgcagc
     7021 tgggcccggc cggcggccgg ctggagctgg ttcgccaccg ggaccgggac cgcgaaatcc
     7081 tggccgcgct gccctggagc gggaccgcct ggctgagagt ccgcacctcc gggcaggagt
     7141 actcctttca ggcctcggtt gacggagaac gctggcagcc ggtcgggccg cccctggacg
     7201 gccgatttct cgcaccggaa tcgataatga cgggctccct gaaacgcaag tgttttaccg
     7261 gccttcggat cgggatattc aacgagagcg atccggccga aacgaccgag ttcagccggt
     7321 tcgagtaccg ggccgctgcc ggcggctgaa tccgccgacg gatttgaccg gttgaccaaa
     7381 aaggagaagc agaatgtgga aaaacaagct gaagattacg gcggcggcgc tcctcatggc
     7441 cgctgaactg gccgccggcg gcctggccgg cgccgatgaa cccgggctgc tcttcgggcc
     7501 gaccgttacc ctccgccaga atgccgacgg cgtccggacg ctggaagatc tcaaggcggt
     7561 tgacggaaac tttctgctgg ccttccagca ggacctgggc gagaacttca tgaagctttg
     7621 cgaggcggcc ggggtgacgg tctatctgaa ttcggtcggc cgcccggcgc tgcggccggg
     7681 agccgatccc ggtgattact accagcactc gatcttcggc gcttttggac gcgactggag
     7741 ggagttcctg ctgcgcaacg ccggccgcca ggaccttcac ggcctctgct gcgtgccgga
     7801 cgaattcacc tggcacaacg cccagctgca gtacaccctt tccatccggc cgccgcgcga
     7861 ctggcccttt tacccaagcg acgaaaccat cggccggcgc ttcaagcagg agaccggctt
     7921 ggagctgccc cggttcggcc attcgcgggt tctggacggc cgggatcccg cccagcgcag
     7981 ctacgcggcg atccgctatc gctggacggc cgaggtcctc aaggagtggt ccgaccagat
     8041 ccggtccgtc aacccgcggt taaaggtcgg cgcggtcctg accaccctgc ccgcctacgg
     8101 cctggaacgc tacccgtccg ggatcgcctg ggaccggctg ggtgagagcg gggcgcttga
     8161 cctggcggtg gtgaccgcct tcgggaccgg ccacgactac cggggcctgg atacccacta
     8221 cctgatctcc gagacggccg cgaccctggc ggccgccttc gg
//
LOCUS       DAOQYN010000074         8142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9299_length_8142_cov_4.578954, whole genome shotgun sequence.
ACCESSION   DAOQYN010000074 DAOQYN010000000
VERSION     DAOQYN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9299_length_8142_cov_4.578954"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKN80_04180"
     CDS             complement(<1..265)
                     /locus_tag="PKN80_04180"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484216.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNQ35243.1"
                     /translation="MVRTIKIFDTTLRDGEQSPGASLTPEAKLLVARQLEKLGVDIIE
                     AGFPISSPGDFNSVQAVARLVKKCEVAALARALRPVDLDLVGGM"
     gene            481..1905
                     /locus_tag="PKN80_04185"
     CDS             481..1905
                     /locus_tag="PKN80_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ35244.1"
                     /translation="MKKGLKEPELIKKIGEFAASAGWDAYLVGGYLRDRILRRACHDL
                     DLVVIGDATRFARDLARRLEVAPPMVYGRFGTAMLRVGHITLELASARRESYQPDSRK
                     PRVEPANLAEDLRRRDFTINALAQSLSDGRLVDLFQGRQDIRNRVIRTPVEADKTFFD
                     DPLRMLRAVRFAARLNFRIDPETAAAIRRNVDRLKIVSRERIAEEFLKIMASPAPAQA
                     ILLMNDLRLLPLVLPEIENLKNVVAADDRECKNVFRHTLIVLDRVSRKTRDLPTRLGA
                     LFHDIGKPATQRYVPGEGWTFHDHPLVGSRIFQEIARRFNLGAPVRNKVSRLIEFHLR
                     PHYLAGAGVEEDGATGRAINHLVNDSGPYLASLFILAKADLTSRNENKVRRGLAEIEA
                     LEEKIRIWKRQQRRATFRLAIDGNDIMSLLALKPGPEVGKIKTELEQAVLEGKLPNQK
                     RALKKSLKSGYNSLKITIRQKEGL"
     gene            1908..2540
                     /locus_tag="PKN80_04190"
     CDS             1908..2540
                     /locus_tag="PKN80_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35245.1"
                     /translation="MNKKLDRKRVTAVIIGLVVLVAAIITAITIRSTSKRADWSRLLP
                     EETVRQAFDYGRGHRQDLMVDFQAPWTVLLGYDRDKGRAVIYTPAHCLAQMVRNLDPG
                     EPATDTASVCDLAGGYINSVWFGVELYGEDTGFDSQFKASVLVDTQVVEPIKTVYRGS
                     ESAREYTVISRHDYHFPVSILDGRKRIILRINRAEGQQPIDFPFDLDRIP"
     gene            complement(2556..4664)
                     /locus_tag="PKN80_04195"
     CDS             complement(2556..4664)
                     /locus_tag="PKN80_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNQ35246.1"
                     /translation="MKETREAIGREIAIVGYATLGLFFIIALFSFSPFDPTLPIFNNF
                     PLNRPPVNLAGLAGAFLAGLLVNFLGWPAFLAPFLFVFYAYQSYAREEPLAPGRLFFA
                     LLALVLLSGLVALAFQDRFSAAVTGREGFLTLVAPGGVAGCLLFRWGSSWLGSIGAAL
                     IYSALLLLLIFYALSLSLRPLSVPENRPAARKRAAPVPANAEPVFVPATTLEKPEKET
                     AAPASGAGAPVPVLPPVPSGPADGNYHLPPLDLLIPAKEDSRPEFDLDRYKSVIEDTI
                     ADFGILVKAVEANVGPTVTMFELELSPGVMPQRIVSLQDNLAMALRATSVRVVAPLPG
                     KSTVGVEVPNPTTSTVYLSEIVGAPEFGQHRSKIALALGKDTLGRPLIADLRNMPHIL
                     IAGATGSGKTVCLNSLLISFLYRATPEEVQLVLIDPKMVEMSVYNELPHLLLPVVTEA
                     RSAIDALKWMVGEMERRYRLFSQNNVRSIDVYNSRDVERIPYIVIVIDELADLITVAR
                     NDVESNIMRLSQLARAAGIHLIIATQRPSVNVITGVIKANLPARIAFQVSSKVDSRTI
                     LDANGAEKLLGRGDMLYLAPGALHPIRSQGCYLSDQEMERVCEFWKRQGRGPTYDLSY
                     IQEGAEAASEFGGGEADDPLFNEAVKIVLTHQLASISLIQRRLKIGFNRSARLIEEME
                     KQGIVGPYREGKPREILKKS"
     gene            4816..5211
                     /gene="rbfA"
                     /locus_tag="PKN80_04200"
     CDS             4816..5211
                     /gene="rbfA"
                     /locus_tag="PKN80_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNQ35247.1"
                     /translation="MKRRNLRVAGLIHRLLAEALQKELTETTAWKINITKVDLSPDLR
                     HARVFFLAITGPEDRLECEQMLKANTPVLKKAMARNLRVRFQPEISFVWDEALQATER
                     VDEILDRLSRERRATEVPASETEDKRTDD"
     gene            5204..6865
                     /gene="argS"
                     /locus_tag="PKN80_04205"
     CDS             5204..6865
                     /gene="argS"
                     /locus_tag="PKN80_04205"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNQ35248.1"
                     /translation="MTDIVEALRTALEEIAAELHREGELERPLPVGENDFVIPRERQR
                     GDLATPVLLKAPSRRPRPELAVLFGERLRARALPFLQSISVAGGGYLNFLLTPSALAD
                     NLRQCLNNPDQLVPPAETRLKVRVEFVSANPTGPLTIAHGRQAAFGEALARILSACGH
                     EVKREYYLNDEGRQIDLLGESLRVRVLNLLGDARPLPEDGYQGEYLVDLARGLVAERG
                     ESLKLETGEFFRDRASSEILQEIRSDLASFGVIFDSYRSQKEITARGEVGETLERLKK
                     AGLVYESEGALFLKTMPYGDDKDRVLVKSDGSYTYLAPDLAYHRLKYAEGDQLMVNLW
                     GPDHFGYITRLQAGLAGLGCDPQKLKIIIVQLTTLYRDGEKVKMSTRQGEFLPLSLLT
                     RELGADVTKFFFLSRKASSHLDFDLEQAKKNSAENPVFYLQYASARIASLFRHQEEKM
                     PGADLSETGDLLERLGEPEERELMLRLGQYGSAVAMAGRNLEPQLLLAYLMNLVKIFH
                     QYYQRCQIIGIDPDLSRARLLLVRGLEKVLKQGLHLLNIRAPERM"
     gene            6917..>8142
                     /locus_tag="PKN80_04210"
     CDS             6917..>8142
                     /locus_tag="PKN80_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNQ35249.1"
                     /translation="MIARKQFLKLLENIGFLNKAQIDRAIKEHSMGSRSIRSVLLDLK
                     YLGGEESSAALMPQLGLVPTPINITNLPASLVEKVPSNFAREHRVVPLRMDGTTLILA
                     TDEPLNILVEENLADNLNVKDVRLEITTTESLNNSLLNAYSLTNEDLRPLLEKVDKGY
                     TGELDMDNPALTVKSGVDGETEEALQAPVIRLANAVIVDALRRRASDIHVEPMEDRFR
                     IRYRIDGVLHENINPPRNLQNPLLARFKIMAGMDLAEKRLPQDGRIMINAAGRPIDLR
                     VSSLPGIYGESLVMRILDKTSMLLSMDQLGFLEDDQQKWAELLKYSGGIVLVTGPTGS
                     GKTTTLYTSLSALNTTDVKIMTVEEPVEYQIEGINQTPVRTDIGLTFGRILRSFLRQA
                     PDIILVGEIRDFETAD"
BASE COUNT         1751 a         2532 c         2456 g         1403 t
ORIGIN      
        1 gcatgccgcc gacgagatcc aggtcgacgg gccgcagcgc ccgggccaag gcggccacct
       61 cacacttctt caccagccgg gccaccgcct gaacggaatt aaaatccccc ggagaggaga
      121 ttggaaaacc ggcctcgatg atatcaacgc cgagcttttc cagctggcgg gccaccagca
      181 gcttggcctc cggggtcagg ctggcacccg gagactgctc cccatccctc agggtagtat
      241 caaaaatttt tattgttcgt accataagaa taattgtaac atagaaaacc ggcttcttca
      301 acaacggcgg ggcgggccgg cgtgatgatt aaaggccggt ttttcagcgg tcactcccgc
      361 gggcatgacg ttttttttga ctttcgcggc aggcgggatc gcaccgcgcc aacgaatcaa
      421 tccgccggtt ggacgtggtc attgagcgga cgggcggctt tttgtggtaa aatttatggc
      481 gtgaaaaaag gcctcaaaga accggagctc atcaagaaga tcggcgaatt tgccgcttcg
      541 gccggttggg acgcctacct ggtgggcggt tacctgcgcg accggatcct gcgccgtgcc
      601 tgccacgacc tggacctggt ggtcatcggt gacgccaccc gcttcgcccg ggacctcgcc
      661 cgccggctgg aagtggcgcc gccaatggtt tacggccgtt tcggaaccgc catgctgcgg
      721 gttggacaca taaccctgga gctggcctcc gcccgacgcg aaagctacca gcccgattcc
      781 aggaaacccc gcgtggaacc ggccaatctg gccgaggacc tccggcgccg cgattttacc
      841 atcaacgccc tggcccaatc actttcagac ggccgcctgg tggatctctt ccagggccgc
      901 caggacatca gaaaccgggt catccggacc ccggttgaag ccgacaagac cttcttcgac
      961 gacccgctgc ggatgctccg ggcggtccgt tttgccgccc gcctcaactt ccgcattgac
     1021 ccggagacgg ccgccgccat tcgccgcaat gtcgaccggc tcaagatcgt cagccgggag
     1081 cgaatcgccg aggaatttct caaaataatg gcaagcccgg cgccggccca ggctattctg
     1141 ctcatgaacg accttaggct gctgcccctg gtcctgccgg agatcgagaa cctcaaaaac
     1201 gtggtggccg ccgatgaccg ggagtgcaag aacgtcttcc gccacaccct gatcgtgctg
     1261 gaccgggtaa gccggaagac ccgggacctc ccgacccggc tgggggcgct gtttcacgat
     1321 atcggcaaac cggccaccca gcgttacgta cccggcgagg gctggacctt tcatgatcac
     1381 ccgctggtcg gcagccgaat cttccaggag atcgcccggc gcttcaacct gggcgccccg
     1441 gtccgaaaca aggtctcccg gctgatcgaa ttccacctgc gcccccatta cctggccgga
     1501 gcgggcgtgg aggaggatgg ggccaccggg cgggccatca atcacctggt caatgactcc
     1561 ggaccctacc tggcctcgct cttcatcctg gccaaggccg atctgaccag ccgcaacgag
     1621 aacaaggtcc ggcgcggcct ggccgaaatc gaggcgctgg aagagaagat ccggatctgg
     1681 aaacgacagc agcgacgggc tactttccgg ctggccatcg acggcaatga catcatgtcc
     1741 ctgctcgcct tgaaacccgg accggaagtg gggaagatca agacggaact ggagcaggcg
     1801 gtgctggagg gaaagctgcc caaccagaaa agggctttga aaaaatcgct gaaaagcggt
     1861 tataattctt taaaaataac cataagacaa aaggaaggcc tttaaaaatg aacaagaagc
     1921 ttgacaggaa acgggtcacg gccgtcataa tcggactggt ggttcttgtc gcggcgataa
     1981 taacggcaat cacaatccgg agcacttcca aacgcgccga ctggagccgt ctgcttccgg
     2041 aggagacggt ccggcaggca ttcgactacg ggaggggtca ccgccaggac ctgatggttg
     2101 acttccaggc accttggacg gtcctgctcg gttacgaccg ggacaaggga cgggcggtca
     2161 tctatactcc ggcccactgc ctggcccaga tggttcgcaa cctggacccg ggagaaccgg
     2221 ccactgatac cgcctcggtc tgcgacctgg ccggcggata catcaacagc gtgtggttcg
     2281 gggtggaact ttatggcgag gataccgggt tcgactccca gttcaaagcc tcggtcctgg
     2341 tcgataccca ggtggtggaa cccatcaaaa ccgtctaccg gggatcagaa tccgcgcggg
     2401 aatacacggt catcagccgt cacgattatc atttcccggt ttccatcctg gacggccgga
     2461 agagaatcat cctgcgcatc aaccgggccg agggacagca accgatcgat ttcccgtttg
     2521 acctggatag aatcccctga acgcggccgc cggattcagc tcttcttaag gatttcgcgc
     2581 ggcttgcctt cacggtaggg cccgacgatt ccctgtttct ccatctcctc gatgagccga
     2641 gccgaacggt tgaacccgat cttaaggcgc cgctggatca gggaaatcga agccagctgg
     2701 tgggtaagga ctatcttcac cgcttcatta aacaggggat catccgcctc gccgccgccg
     2761 aattccgaag ccgcctcggc tccttcctgg atgtaactca gatcgtaggt gggccccctc
     2821 ccctgccgct tccagaactc gcagacccgt tccatctcct ggtcgctcag gtaacaaccc
     2881 tgggaacgga tcggatgaag ggccccgggc gccaggtaga gcatgtcgcc ccggccgagc
     2941 agcttctcgg cgccattggc atccaggatg gtccggctgt cgaccttgga ggagacctgg
     3001 aaggcaatcc gggccggcag gttggcctta atgaccccgg tgattacgtt gaccgaagga
     3061 cgctgggtgg cgataatcag gtggatgccg gccgcccggg ccagctggga gaggcgcatg
     3121 atattgctct ccacgtcgtt gcgggcaacg gtgatcagat cggccagctc gtcgatcacg
     3181 atcacgatgt agggaatgcg ttccacgtcg cggctgttat agacatctat gctgcggacg
     3241 ttgttctggg agaagagccg gtagcggcgt tccatctccc cgaccatcca cttcagggcg
     3301 tcgatggcgc tgcgcgcctc ggtaaccacc ggcagcagca ggtgaggcag ctcgttatag
     3361 acgctcattt cgaccatctt ggggtcaatg agcaccagct ggacctcctc gggcgtggcc
     3421 cggtacagaa aagagatcag caggctgttc aggcagacgg tcttgccgct gccggtggcc
     3481 ccggcaatca ggatgtgagg catgttgcgc agatcggcga tcagcggccg gccgagggtg
     3541 tccttaccca gggccaaggc gatcttggaa cgatgctggc cgaactccgg ggcgccgact
     3601 atctccgaaa ggtagacggt cgaggtcgtg ggattgggaa cttccacgcc aacggtcgac
     3661 ttgccgggca acggggcgac cacccgcacg ctggtggccc gcagggccat ggccaggttg
     3721 tcctggaggc tgacgatgcg ctgtggcatc accccgggtg aaagttccag ctcgaacatg
     3781 gttacggtgg ggccgacatt ggcctcaacc gccttgacca gaattccaaa atcggcaatg
     3841 gtatcctcga tgaccgactt gtagcggtcg aggtcgaact cgggccggga gtcctccttg
     3901 gccgggatca gcagatccag cggcggcagg tggtaattgc cgtcggccgg cccggagggc
     3961 accgggggca gtaccggtac cggggccccg gcaccggagg caggggcggc cgtttccttt
     4021 tcgggctttt ccagcgtggt ggccggtacg aagaccggtt ccgcattggc tggaacggga
     4081 gcggcccgtt tgcgggccgc cggccggttt tcggggaccg aaagcgggcg cagggaaagc
     4141 gaaagggcat agaagatcag cagcaggagg agtgccgagt aaatcagggc cgctccgatc
     4201 gaacccagcc agctggaacc ccagcggaaa agaagacaac cggccacccc tccgggcgcc
     4261 accagggtga ggaatccttc ccgcccggtg accgcggcgg aaaaacgatc ctggaaggcg
     4321 agcgcgacca ggccgctcaa aaggaccagg gccagcaggg cgaaaaagag ccggcccggc
     4381 gccagcggtt cctcccgggc gtaagactgg taggcgtaaa aaacaaaaag aaagggggcc
     4441 agaaaagcgg gccagcccag aaagtttacc agcagcccgg ccaggaaggc accggccagt
     4501 ccagccaggt tgaccggcgg ccggttaagc ggaaagttgt tgaagatcgg cagggtcggg
     4561 tcaaagggag agaacgagaa gagcgcaatt ataaaaaaca ggccaagagt ggcgtagccg
     4621 acgatcgcga tctcccggcc gatggcttca cgagtctcct tcatctggct tccttttacc
     4681 ctccggcccc gggaatgacg taacttgcgg ggccactcca attatactat aattaaaaga
     4741 tatcaacaaa aaaacacccg tccacgcggg aaagggcgcc agtcgaggcg tccgtcccga
     4801 aaacctgacc cgaagatgaa acgaagaaac ctgcgtgtgg ccggcctgat tcaccgtctc
     4861 ctggccgagg cgctccagaa agaacttacc gagacgaccg cctggaagat caacatcacc
     4921 aaggttgacc tttcgcccga tctgcgccat gcccgggtct tcttcctggc gataaccggg
     4981 ccggaagacc ggctggaatg cgagcagatg ctcaaggcca acaccccggt cctaaaaaaa
     5041 gccatggccc ggaatctaag ggtacgtttc cagcccgaaa tcagctttgt ctgggacgag
     5101 gcgctccagg cgaccgaacg ggttgacgaa atcctggaca ggttgagccg cgaacgccgg
     5161 gccaccgaag tcccggcatc cgaaaccgaa gataagcgaa ccgatgactg atatcgtgga
     5221 agccctccgg accgcccttg aggagatcgc cgccgaactg catcgggagg gggaactcga
     5281 aagaccgctg ccggtgggag agaatgattt tgtcattccc cgcgaaagac agcggggaga
     5341 cctggccacc ccggtcctgc tcaaggcacc gagccgccgg ccgcggccgg agctggcggt
     5401 gctcttcgga gaacggctgc gcgcccgggc actccccttc ctccaatcca tctcggtggc
     5461 tggcggcggt tacctgaact tcctgctcac cccctcggcg ctggccgaca acctgcgcca
     5521 gtgcctcaac aaccccgacc agctggtacc tccggccgaa acccggctca aggttcgggt
     5581 ggagtttgtg agtgccaacc cgaccgggcc gctcaccatc gcccatggac gccaggccgc
     5641 ctttggcgaa gccctggccc gcattctgtc cgcctgcggc cacgaggtaa agcgcgaata
     5701 ctacctgaac gacgaagggc ggcagatcga cctgctcggc gaatcactgc gggtgcgcgt
     5761 cctgaacctg ctgggagatg cccggcccct gccggaagac ggttaccagg gtgaatacct
     5821 ggttgacctg gcccgcggcc tggtggccga acgcggggaa agtctgaaat tggaaacggg
     5881 cgaattcttc cgtgaccggg ccagttcgga aatactgcag gaaatccgtt ccgacctggc
     5941 ctcgttcggc gtgatcttcg actcctaccg gagccagaag gaaatcaccg cgcgcgggga
     6001 ggtcggggag acgctggaac gccttaaaaa agcggggctc gtctatgaat ctgagggggc
     6061 actctttctg aagacgatgc cctatggaga cgacaaggac cgggtcctgg tcaagtccga
     6121 cggcagctac acctacctgg cccccgacct cgcctatcac cggctcaagt acgccgaggg
     6181 cgaccaactg atggtcaacc tctgggggcc cgatcatttc ggatacataa cccgactcca
     6241 ggcgggcctg gccggcctgg gctgcgaccc gcagaagctg aagatcatca tcgtccagct
     6301 gacgaccctt taccgggacg gcgaaaaggt caagatgtcc acccggcagg gggaattcct
     6361 gcccctctcc ctgctgacca gggaactggg agcggacgtg accaaattct tctttctctc
     6421 gcgtaaggcc agcagccatc tggatttcga cctggaacag gccaagaaaa attcggccga
     6481 gaacccggtc ttttacctcc agtacgccag cgcccggatt gcctccctct ttcgccacca
     6541 ggaggagaag atgcccgggg ccgacctttc ggaaaccggc gacctcctcg aacgcctcgg
     6601 ggaaccggaa gaacgggaac tgatgctgcg cctgggtcag tacggatcgg cggtcgccat
     6661 ggcggggcga aacctggaac cgcaattgct cctcgcttac ctcatgaacc tggtcaagat
     6721 ctttcaccag tactatcagc gctgccagat aataggaatc gatccggatt taagccgggc
     6781 ccgcctcctg ctggtccggg gactggaaaa agtcctgaaa caggggctgc acctcttaaa
     6841 catcagggct ccggaacgca tgtaaccttg acaggaagcc ggtttttttc ctacaatatg
     6901 accggagtcc aaaacaatga tcgcccggaa acaattctta aaactcctgg aaaacatcgg
     6961 cttccttaac aaggcccaga ttgaccgggc catcaaggaa cattcgatgg gttcccgttc
     7021 cataaggtcg gtcctgctgg accttaagta ccttggcggc gaggagtcga gcgcggccct
     7081 gatgcctcaa ttggggctgg tgcccacccc gatcaacatc accaacctgc cggcttcgct
     7141 ggtcgagaag gtgccctcga atttcgcccg cgaacaccgg gtcgtcccgc tccgaatgga
     7201 cggtaccacc ctgatcctgg ccaccgacga accgctcaac atcctcgtcg aggaaaacct
     7261 ggccgacaat ctcaacgtca aggatgtccg gctggaaatc accaccaccg aaagcctgaa
     7321 caattcgctc ctcaacgctt acagtttgac caacgaggac cttcgccccc tcctggaaaa
     7381 ggtggacaag ggttacaccg gcgaactgga catggataac ccggccctga cggtcaagtc
     7441 aggggtcgac ggcgaaaccg aagaagccct gcaggcgccg gtgattcggc tggccaacgc
     7501 ggtcatcgtc gatgccctgc gccgccgggc cagcgatatc cacgtggaac cgatggagga
     7561 ccgtttccgc atacgctacc gcatcgacgg agtcctccac gaaaacatca atccgccgcg
     7621 caacctgcag aacccgctgc tggcccgttt caagatcatg gccggcatgg acctggccga
     7681 aaaacggctg ccccaggacg gccggatcat gatcaatgcc gccggacggc cgatcgatct
     7741 gcgcgtcagc tcgctgcccg gcatctacgg ggaaagcctg gtcatgcgta tcctggacaa
     7801 gacctcgatg ctgctctcca tggaccagct gggctttctg gaagacgatc agcagaagtg
     7861 ggcggaactg ctcaagtact cgggcggcat cgtactggta accgggccca ccggcagcgg
     7921 taagaccacc acgctctaca cctctctctc cgcgctcaac acaaccgacg tgaagatcat
     7981 gaccgtcgag gagccggtcg agtaccagat tgaaggaatc aaccagactc cggtccgtac
     8041 cgacatcggc ctgactttcg gtcgtatcct ccggagtttc ctgcgccagg cgccggacat
     8101 catcctggtg ggtgaaatcc gcgacttcga aaccgctgat at
//
LOCUS       DAOQYN010000075         8126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9322_length_8126_cov_4.224384, whole genome shotgun sequence.
ACCESSION   DAOQYN010000075 DAOQYN010000000
VERSION     DAOQYN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9322_length_8126_cov_4.224384"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /gene="amrB"
                     /locus_tag="PKN80_04215"
     CDS             complement(<1..1453)
                     /gene="amrB"
                     /locus_tag="PKN80_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNQ35250.1"
                     /translation="MEFAGAWGMLMLLAGTLRAGTRPPAVAGQFYPSDRRELTRLVDG
                     YLKAADPAQMPAPVLALLVPHAGYPYSGPTAAFGYKLLSGRKLDTVILLGNSHRYPLR
                     KGAVYLEGEFLTPLGNFAVNTRLAQKLVAASPYLQADSEPHRLEHSLEVQLPFLERTA
                     GRCRIVPILMGEASLEACRQIGETLAAALRELNLAEKTVIIASSDLSHYPPAAEARRL
                     DQAFLAELAEMDPEKLAARVARLEKEPVPDLACVICSQQALYTAIYAARALGANQARV
                     LNYTNSGERTGERGRSVGYAAAAFYRAAGAAQTKTAAPKADPAPEPGFSLDADDRKTL
                     LALARQSIENVLQNGRLLEYRTNDPELIRPAAVFVTLTSAGRLRGCIGTTAAEEPLAR
                     AVIRLAAAAAFQDPRFNPVRAAELPGLKIEISVLSPLRRAASFKEIKPGLHGVTVRRD
                     GRTGLFLPQVWEQIPDRAEFLTELCRQKTGLNPE"
     gene            complement(1480..2616)
                     /gene="amrS"
                     /locus_tag="PKN80_04220"
     CDS             complement(1480..2616)
                     /gene="amrS"
                     /locus_tag="PKN80_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNQ35251.1"
                     /translation="MTGSGQLKRRAAGRIFTILTACCFAIMAAGLAARDAAGQDPAAG
                     AAARHYQARPENQVQCRLCPRECLIPAGGSGYCTVRRNENGRLRSLVYGQACSLNLDP
                     VEKKPLYHFLPGTQTLSMAAIGCNLRCSFCQNYSISMARPGEVESLPLRPERLAALAR
                     EKSAPSISYTYSEPTAYYEYLYDSAALARKAGIRNVWVTCGYINPEPLEELCGVLDAA
                     NVDLKGFSDGVYRRVAGAKLAPVLATLATLKRKGVWLEVGYLVIPTLNDGPKELADFC
                     AWTVKNLGPETPVHFLRFFPRYQMTNLPATPVATLERACQAARAAGSQFVYIGNVPGH
                     RANHTYCPGCETLLIERKGYFIQQNHLKNGRCPKCGRKIPGVWS"
     gene            2836..5007
                     /locus_tag="PKN80_04225"
     CDS             2836..5007
                     /locus_tag="PKN80_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35252.1"
                     /translation="MPNLVFFLSGLLAVIGQTLLLREMLVLFQGNELVLGLLLSFWLV
                     GGAAGSWLAGRARGGRLRSGLARAFAGAGLGLAFGFGLVRFGRPLLGLLPGLGVPMAA
                     TLLIAGLVFLPPSFCFGRQFGLAVRWNQAFFRSRPAASVYFWEALGYLSGGLFYTFLL
                     LERLEPVRIISLLLAACFTVSLFLSGLSRRLRRAGLFLTVLLLAAGFRPGLEAHLLQA
                     ACPGFQLRGVANSPYGQLLSVSREGQESWFYNGLPLTHPDPARQEELGLLPWLLHPSG
                     RVLFLRGLDSLPELPGTEIVQLIGDRGLYDLFGRAGGSSPYRDFTSRPGEVSARSLPA
                     VRDFLRRDRGSYGLIIFDLTAPLTLAQNRYFTREFFSLLSERLAPGGLVAISLPGSLV
                     HYGPELTELNRVLYSTLAGVFGRVRVLPGDQENLFLAGPPDLDWEIGRVLGRFRALER
                     PTRYLSDGYLAYRLDPARLAWFQDQVLAGPAAPPNLDFQPRLLLAGLRYWQSFFTPAT
                     GRLYRRLVDYTGLLWLLPLLWLAAGRRPGPAGSLFSSGFAGLSLQMLILWAFQVRYAA
                     LYQWLGLLSALFMAGLALGSFAGRRLAGFLSPAAAFRWLESAFLLWLGLAWLLFHLQF
                     LSRPALFLLTAGSGFLVGGQFPIGVACLAAGGPEGPPAGRLYAADLLGGWLAALLVGG
                     LLIPAWGLEPVFLFLLALKLASWRGWAFGAAPALTPPAGLR"
     gene            5063..>6213
                     /locus_tag="PKN80_04230"
     CDS             5063..>6213
                     /locus_tag="PKN80_04230"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNQ35253.1"
                     /translation="MKVDFPVQPDWLAGLEPAGRQDFSVAAIYFPGFHPTPFFEKHFG
                     PGWSEWELVRSARPRFPGHRQPLEPLWGCYDESDPAWAEREIEAAAGHGIDIFIFDWY
                     WYQGVEILNEALERGFLAASNRNRLRFGLMWANHTIVNVFPAPVGRPPAELLPIVHTP
                     EDLDRVIDHCARNFFVQPNYWFIEGKPWFGFFNLGALLKSLGGLSGAAAGIERMRRRA
                     ERAGFNGLYLGGFTGGADGAETFRKLGFDHVTTYNIVTPEGGRPGRALNPYREVAAAH
                     PARWREIAGAGLPCWPVVTQGWDVSPRNSPGEPWPPARWEWPFGALIPDNSPELFGRL
                     CSAARRFLSGQPGPARVMLLNAWNEWTESSVLAPTRDQGFACLEALREA"
     assembly_gap    6214..6223
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6261..7352
                     /locus_tag="PKN80_04235"
     CDS             6261..7352
                     /locus_tag="PKN80_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35254.1"
                     /translation="MKEIKTGVIGCGNISGTYLKNFRESFPFIRVEAVSDLDRARAEG
                     RAAEFGVPRVLETAELLADPELELVVNLTVPAGHGPLNRAALEAGKHVYCEKPLALDR
                     ETGLALAGLARSKNLRLGGAPDTFLGAGYQTARRRLDEGLIGVPVAATAFMVCRGHES
                     WHPAPDFYYKPGGGPLFDMGPYYLTALVYLLGPVARVTGLARASFPERTITSQPDAGQ
                     KIKVEVATHIIGGLEFASGALATLVTSFDVWGGQLPRLEVYGSEGSLSLPDPNRFAAP
                     ILHLPGGRGQSWSEIPLDPGYTANSRGLGTADLVRGLFEGRPHLASGELALHVLDIMQ
                     SILEAAASGRSVKLATACERPGRLDPDFK"
     gene            7372..8022
                     /locus_tag="PKN80_04240"
     CDS             7372..8022
                     /locus_tag="PKN80_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006328624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNQ35255.1"
                     /translation="MAQKEALIVRGGWKGHEPVETSDIVAAFLTGEGWKVEISENLDV
                     YLDQEKLARLNLIVPVWTMGEIAKPQLAGLLTAVRGGVGLAGWHGGLADSFRSQTEYQ
                     FMVGGQWVAHPGGIIDYTVNIIRPDDPITAGLKDFRMRSEQYYLHVDPGNEVLATTTF
                     GGEHAEWIAGTVMPAVWKRRYGQGRVFYSSLGHVAADFQVPEAMEILRRGLLWAAR"
BASE COUNT         1072 a         2892 c         2682 g         1470 t
ORIGIN      
        1 cctccgggtt caggccggtc ttctgccggc agagttcggt caggaattca gcccggtccg
       61 ggatttgctc ccagacctgg ggcaggaaga ggccggtgcg gccgtcccgg cgtaccgtca
      121 ccccgtggag gccgggcttg atttccttga aggaagcggc tcggcgcaag ggtgagagga
      181 ccgagatttc aatcttgagt cccggcaatt cggcggcccg gaccgggttg aagcgcggat
      241 cctgaaaggc ggccgccgcc gccagccgga tgaccgcccg ggccagcggc tcctcggcgg
      301 cggtcgtgcc gatgcagccg cgcagccggc cggcactggt cagggtgacg aagaccgcgg
      361 ccggccggat cagctccgga tcgttggtcc ggtactccag cagccggccg ttctggagca
      421 cattctcaat ggactgccgg gccagcgcca ggagcgtctt ccggtcatca gcgtcgaggc
      481 tgaagccggg ttccggcgcc ggatccgcct tcggcgcggc cgtttttgtt tgcgccgcac
      541 cggccgctcg gtagaaggcc gcggccgcgt acccgaccga ccggccgcgt tccccggtcc
      601 gttccccgga attggtgtag ttaagcaccc tggcctggtt ggcgcccagc gcccgggccg
      661 cgtaaatggc ggtgtaaagc gcctgctggc tgcagatcac gcaggccagg tcagggaccg
      721 gttccttctc cagccgggcc acccgggccg ccagcttctc cggatccatt tcggccagct
      781 ccgcgaggaa ggcctggtca agccgccggg cttcggccgc cggcggatag tgggagagat
      841 ccgaggaggc aatgatcacc gttttctcgg ccaggttcag ttccctcagc gcggccgcca
      901 gggtttcgcc gatctgccgg caggcctcca gggaggcctc gcccatgagg atgggcacga
      961 tccggcaacg gccggccgtg cgttccagga agggcagctg gacctccagg gaatgctcga
     1021 gccggtgcgg ttccgaatcg gcctgcaggt aaggcgaggc ggccaccagc ttctgggcca
     1081 gtcgggtgtt caccgcgaag ttgcccagcg gggtgaggaa ctccccctcc aggtaaacgg
     1141 ctcccttccg gagtgggtaa cggtggctgt tgccgagcag gatcaccgtg tccagcttgc
     1201 ggccggacag cagcttgtag ccgaaggcgg ccgtcggccc cgagtaggga tagccggcgt
     1261 ggggcaccag cagggccagg accggcgccg gcatttgggc cgggtcggcc gccttcaggt
     1321 agccgtccac caggcgggtg agttcccggc ggtcggacgg gtagaattgc ccggccaccg
     1381 ccggcggccg cgtcccggcc cggagcgttc ccgccagcag catcagcatc ccccaggcgc
     1441 ccgcgaattc caaccaccga cctcccgcca tggcaccgtt catgaccaca cccccggtat
     1501 cttccggccg catttcggac agcggccatt cttgagatgg ttctgctgaa tgaagtaccc
     1561 cttccgttcg atcagtagcg tttcgcaccc ggggcagtag gtgtggttgg cccggtggcc
     1621 gggcacgttg ccgatataga cgaactggct gccggccgct cgggccgcct ggcacgcccg
     1681 ttccagggtg gccaccggcg tggccggcag gttggtcatc tggtagcggg gaaagaaacg
     1741 caggaagtgg accggcgttt ccggccccag gttttttacc gtccaggcgc agaaatccgc
     1801 gagttccttg ggaccgtcgt tgagcgtcgg gataaccagg taaccgacct ccagccagac
     1861 ccccttgcgc ttgagcgtgg ccagcgttgc cagcaccggc gccagcttcg ccccggccac
     1921 ccggcggtaa acgccgtcgg agaagccctt caggtccacg ttggccgcgt ccagcactcc
     1981 gcaaagctcc tcgagcggtt cggggttgat gtagccgcag gtgacccaga cgttgcggat
     2041 gcccgccttg cgggcgagcg cggcggagtc gtacaggtac tcgtagtaag cggtcggttc
     2101 gctgtaggtg taggagatcg agggggccga cttctcccgg gccagggccg ccagccgttc
     2161 gggccggagc gggaggctct ccacctcgcc gggccgggcc atcgagattg agtagttctg
     2221 gcagaaggag cagcgcaggt tgcagccaat cgcggccatc gaaagggtct gggttccggg
     2281 caggaaatgg tagaggggct tcttttcgac cgggtccagg ttcaggctgc aggcctggcc
     2341 gtagaccagg ctgcgcagtc ggccgttttc attccgccgc accgtgcagt agccgctccc
     2401 gccggccggg atcaggcact cgcgcgggca gagccggcac tggacctgat tttcgggtcg
     2461 ggcctggtag tggcgggccg ccgcgccggc ggccgggtcc tggccggccg cgtcccgggc
     2521 cgccagcccg gcagccatga tggcaaaaca gcaggctgtc aaaatggtaa atatccgccc
     2581 cgccgcccgc cgtttgagct gcccgcttcc ggtcatgttt tcccttgttt tatccggccg
     2641 gccccggcct ctttttcacg cttcccgccg gccccgccgg ccgccggccg ttttaagttt
     2701 ggcacaaggc cggcgccctg tcaagttccc gccggccgct gaaattccag ggccttacag
     2761 tgaaccggtc cggaccggtt tccgccggcg ccggttttcc gaccggccgg attctgtgct
     2821 taaatatgga gtgagatgcc gaacctggtc ttttttctct ccggcctcct ggccgtcatc
     2881 ggccagaccc tgcttctgcg ggagatgctg gtcctcttcc agggcaacga actggtcctg
     2941 ggcctgctcc tttccttctg gctggtcggc ggggccgccg gcagctggct ggccggccgc
     3001 gcccggggcg gtcgcttacg ctccgggctg gcccgcgcct tcgccggcgc cggtctcggc
     3061 ctggccttcg gtttcggcct ggtccgcttt ggccggccgc ttctgggcct gctgccgggc
     3121 ctgggtgtgc cgatggccgc cactctgctg atcgccggcc tggttttctt gcctccgagt
     3181 ttttgtttcg gccgccagtt cggcctggcg gtgcgctgga accaggcgtt cttccgctcc
     3241 cggccggccg cctcggtcta cttctgggag gccctgggct atctatccgg cggcctgttc
     3301 tacaccttcc tgctgcttga gcggctcgaa ccggtccgga tcatcagcct gctgctggcc
     3361 gcctgtttca ccgtctccct gttcctctcc ggactctccc ggcggctccg ccgggccggc
     3421 ctgttcctaa cggtcctgct cctggcggcc ggcttccggc ccggcctgga ggcgcacctc
     3481 ctccaggccg cctgccccgg gttccagctc cgcggggttg ccaattctcc ttatggccag
     3541 ctgctctcgg tcagccgcga ggggcaggag tcctggttct acaacgggct tcccctgacc
     3601 catcccgacc cggcccgcca ggaggaactg ggtctgttgc cctggctgct gcatccgtcc
     3661 ggccgggtcc tcttcctgcg cggactcgat tccctgccgg aactgccggg taccgagatc
     3721 gtccagttga tcggcgaccg aggcctctac gacctcttcg gccgggccgg cgggagcagt
     3781 ccttaccgcg atttcaccag ccgccccggg gaagtttcgg cccgctccct gccggccgtc
     3841 cgagacttcc tgcgccggga tcggggatcc tacggcctga ttattttcga cctgaccgcg
     3901 ccgttgactc tggcccagaa ccgttatttt acccgggagt ttttcagcct cctctcggag
     3961 cggctggccc ccggcgggct ggtggcgatc tccctgcccg gctccctggt tcactacggg
     4021 ccggagctga ccgagctcaa ccgggtgctc tattcgaccc tggccggcgt cttcggccgg
     4081 gtgcgggtcc tgcccggcga ccaggagaat ctcttcctgg ccgggccgcc ggatctggat
     4141 tgggagattg gaagggtgct cggccgcttt cgggcgctgg agcggccgac ccgttatctt
     4201 tcagacggct acctggccta ccggctcgat ccggcccggc tggcctggtt ccaggatcag
     4261 gtgctcgccg gcccggcggc gccgcccaac cttgattttc agccccgcct cctgctggcc
     4321 ggcctgcgct actggcagtc cttctttacg ccggccaccg gccgccttta ccgccgcctg
     4381 gtggattata ccggcctgct ctggcttctg ccgctgctct ggctggccgc cggacgccgg
     4441 cccggtccgg ccggcagcct cttttccagc ggcttcgccg gcctgtccct ccagatgctg
     4501 attctctggg ccttccaggt ccggtacgcc gccctctacc agtggctcgg actcctgagc
     4561 gccctcttca tggccggcct ggccctgggc tcgttcgccg gccgccgcct ggccggtttc
     4621 ctgtcgccgg ccgccgcctt ccgctggctg gaatcggcct ttctgctctg gctcgggttg
     4681 gcctggcttc tcttccacct ccagttcctc tcccggccgg ccctcttcct gctgacggcc
     4741 ggctccggct tcctggtcgg cggccagttt ccgattggcg tggcctgcct ggccgccggc
     4801 ggcccggagg ggcccccggc cggccgcctc tatgcggccg acctgctggg cggctggctg
     4861 gccgccctgc tggtcggcgg cctgctcatc ccggcctggg gcctcgagcc ggtcttcctc
     4921 tttctgctcg ccctgaaact ggcctcctgg cgcggttggg ccttcggcgc cgctccagcc
     4981 ttgacgccgc cggcgggctt aaggtaactt aaatcccggt acccccgccg ttattgcgcg
     5041 gacccgcctt caaggagacc cgatgaaagt tgattttccg gttcagccgg actggctggc
     5101 cggcctggaa ccggccggac gccaggactt cagcgtggcc gccatctact ttcccggttt
     5161 tcaccccacg cccttcttcg agaagcattt cggcccgggc tggagcgaat gggaactggt
     5221 ccggtccgcc cggccacgtt ttcccggtca ccggcagccc ctggaacccc tctggggctg
     5281 ctacgacgag tcggacccgg cctgggccga acgggagatc gaggcggccg ccggccacgg
     5341 catcgatatt ttcatctttg actggtactg gtaccagggc gtcgagattc tcaacgaggc
     5401 cctggagcgg ggcttcctgg ccgcttccaa ccgcaaccgc ctccggttcg ggctgatgtg
     5461 ggccaaccac accatcgtca acgtcttccc ggccccggtc ggccggccgc cggccgagct
     5521 gctgcccatc gtccacaccc cggaggatct tgaccgggtg attgaccact gcgcccgcaa
     5581 cttcttcgtc cagcccaact attggttcat cgagggcaag ccctggttcg gcttcttcaa
     5641 cctgggcgcc ctgttaaagt cgctgggcgg cctctccggc gcggccgccg ggatcgagcg
     5701 gatgcgacgc cgggccgaac gggcgggttt caatggcctc tacctgggtg gtttcaccgg
     5761 tggggccgat ggcgccgaga ccttccggaa actcggtttc gaccacgtca ccacctacaa
     5821 catcgtcact cccgagggcg ggcggccggg ccgggcgctc aacccgtacc gggaagtggc
     5881 ggccgcgcac ccggcccgct ggcgggagat cgccggcgcc ggcctgccct gctggccggt
     5941 ggtaacccag ggctgggacg tctcgccccg caacagtccc ggcgaaccct ggccgccggc
     6001 gcgctgggaa tggcccttcg gcgccctgat tccggacaac tccccggaac tcttcggccg
     6061 cctctgttcg gcggcccggc gcttcctctc cggccagccc ggcccggccc gggtgatgct
     6121 gctcaacgcc tggaacgaat ggaccgaaag ctcggtgctg gccccgaccc gggaccaggg
     6181 cttcgcctgc ctcgaggcat tgcgggaggc gttnnnnnnn nnnccccctg accccttcac
     6241 ccttcacgga gaagatgcag atgaaagaga tcaagaccgg cgtgatcggc tgcggcaaca
     6301 tcagcggcac ctacctgaag aacttccggg agagtttccc cttcatccgg gtcgaggccg
     6361 tcagcgacct ggaccgggcc cgggccgaag gccgggccgc cgagttcggc gtgccgcgcg
     6421 tcctggagac cgccgagctc ctggccgatc cggaactgga gctggtcgtc aatcttaccg
     6481 tcccggccgg ccacggcccc ctgaaccggg ccgccctcga ggccggcaag cacgtttact
     6541 gcgagaagcc gctggccctc gaccgggaga ccggcctggc cctggccggc ctggcccggt
     6601 cgaagaacct gcgcctgggc ggcgcgcccg ataccttcct gggcgccggc taccagaccg
     6661 cccgccgccg gctggacgag ggtttgatcg gcgtgccggt ggctgccacc gctttcatgg
     6721 tctgccgggg acacgaaagc tggcatccgg ccccggactt ctactacaaa cccggcggcg
     6781 gccccctctt cgacatgggc ccctactacc tgaccgccct ggtctacctg ctcggcccgg
     6841 tggcccgggt caccggcctg gcccgagcct ccttcccgga acggaccatc accagccagc
     6901 ccgacgccgg tcaaaagatc aaggtggagg tggccaccca catcatcggc ggtctggaat
     6961 tcgcctccgg cgccctggcc accctggtca ccagcttcga cgtctggggc ggccagctgc
     7021 cgcgcctcga ggtttacggc agcgagggat ccctttccct gcccgatccc aaccggttcg
     7081 ccgcccccat cctgcacctg cccggcggcc gcggccagtc ctggtccgaa attccgctcg
     7141 atcccggcta cacggccaac agccgcggcc tgggcaccgc cgacctggtg cgcggcctct
     7201 tcgaggggcg gccccacctg gccagcggcg agctggccct gcacgtgctg gacatcatgc
     7261 agtcaattct tgaggcggcc gcttccggcc gctcggtgaa actggccacc gcctgcgagc
     7321 ggcccggccg tctcgatccc gatttcaaat gacggcaacg gaggcatgaa gatggcgcag
     7381 aaagaggcct tgatcgtgcg gggcggctgg aagggtcacg aaccggtcga aacctcggat
     7441 atcgtcgcgg ctttcctcac cggagagggc tggaaggtgg agatctccga gaacctcgac
     7501 gtatacctgg atcaggagaa actggcccgg ctcaacctga tcgtgccggt ctggacgatg
     7561 ggcgaaatcg ccaagcccca gttggccggc ctgctgaccg cggtccgggg cggggtcggt
     7621 ctcgccggct ggcacggcgg cctggcggac tccttccgca gccagaccga ataccagttc
     7681 atggtcggcg gccagtgggt ggcccacccg ggcggcatca ttgattacac cgtcaacatc
     7741 atccggcccg acgacccgat caccgccggc ctgaaggact tccggatgcg ctccgagcag
     7801 tactacctgc atgtcgaccc cggcaacgag gtcctggcga ccaccacctt cggcggcgaa
     7861 cacgccgaat ggatcgccgg caccgtcatg ccggcggtct ggaagcgccg ctacggccag
     7921 ggccgggtct tctactcctc cctgggccac gtggcggccg acttccaggt gccggaggcc
     7981 atggagattc tccgccgcgg cctactctgg gccgcccggt aagcgggtaa gatgtgctaa
     8041 tatataaacc ggattggctt gttttttggt catccccgcg gatcgtcgag cgggagctgg
     8101 ctggggaggg tctccccgtg cacggt
//
LOCUS       DAOQYN010000076         8106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9353_length_8106_cov_4.547510, whole genome shotgun sequence.
ACCESSION   DAOQYN010000076 DAOQYN010000000
VERSION     DAOQYN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9353_length_8106_cov_4.547510"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..973)
                     /locus_tag="PKN80_04245"
     CDS             complement(161..973)
                     /locus_tag="PKN80_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35256.1"
                     /translation="MKHKKWCLLVLAFLLLGTLTAQASINHGLLGVFDDDVVESIVQI
                     TPGDPLNPTQSAKDWVPCWGVNPWSYDGEWIVYQAAIGTGASEYQRNEICIIRPDGTG
                     WQRLTNNTNTCDNHGNFTPDGNRILFQRYDDSEDYARIWIMDRDGTDQQNLTAVHGGP
                     VNGNECEQKPMVSPDGTKIAFRACVDRDTEEIWVMDIDGTDPIKVSGNINKGNKHSWS
                     PDSQWVLFSADPSENSNSRIYKVRRDGTGLVMLSEEVGDFCENWANWSPDGN"
     gene            complement(1208..2434)
                     /locus_tag="PKN80_04250"
     CDS             complement(1208..2434)
                     /locus_tag="PKN80_04250"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNQ35257.1"
                     /translation="MKRRLPTGSSDGQPGPSRIPWDIKNLSAAPEFEAAPEHDDGEVK
                     AIFYQGLPWRGKGTGVFAYYAIPRAGGKSKIPAMVLVHGGGGSAFVPWVRLWAGRGYA
                     AIAMDTCGCVSGGGYQNHPRHPRGGPPGWGGFDQVDQPIEDQWTCHAVADVILAHSLI
                     RSFPEVDPNKTGLTGISWGGYLTCIAAGVDPRFKFAAPVYGCGFLGDNSIWLDDFRRM
                     GTEKSGRWLRLWDPSVYLPLVKTPLLWVTGPTDAAYPLDSLQKSYRLPPSPRTLCVRV
                     GMTHGHGGPGENPEEIRTRAEARLKGGIDLARITAAGREGRRAFVSFEGRVPVVKAEL
                     NYTTDAGPWRERKWQTIPAELDAAAGRAEGPLPKGATVYYFNLADERRLVVSSEHEEI
                     LTFNGTANNQTTALFT"
     gene            complement(2435..3397)
                     /locus_tag="PKN80_04255"
     CDS             complement(2435..3397)
                     /locus_tag="PKN80_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35258.1"
                     /translation="MRRSVILRLLFTGWLILNGPFPAAAGDVRPLLGVNASLKGYRPF
                     PVDNPWNQDVSGLPADPNSAALIASIGLNKGLHPDFGTVWNNAPIGIPYVIVPGTQPK
                     VPVSFEYAAESDSAPYPIPANPPIEGGPTASGDRHLLMLDRDNRKLYELYAAYPEEGG
                     KRWRAGSGAIFDLTSNALRPAGWTSADAAGLPIFPGLVRYDEVMETGEIRHALRFTCT
                     RTRRAYVFPARHFASRLTDPGLPPMGMRVRLKKNYDTSGFPAAARIILEALKKYGMIL
                     ADNGGDWFITGAPDPRWNDEELATLKRVKGSDLEVVKMAEIILK"
     gene            complement(3429..4592)
                     /locus_tag="PKN80_04260"
     CDS             complement(3429..4592)
                     /locus_tag="PKN80_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNQ35259.1"
                     /translation="MPDLPGKMKYQMVETHEELKKAAQVLAGARSIALDLEGDSMFHY
                     GDTLCLAQVSDGSRTWLIDPLKIKDLSPLSAILGNTGVEKILHGSDYDIRLIRGRLGI
                     NCPPIFDTELAARFLGMARSGLADVLAKRFGVSLDKKFQKDDWSQRPLPDEMLAYAAA
                     DVHWLIRLAEMMKEDLKRLGRLDWVEEECDLLARAPDRRRENGPLHLRFRGAAVMDRR
                     SLAILEAILQMRESLAQERDRPPFKVLDPDAIRQIIQQRPETDQALLRIKGLGRRSEK
                     FRAAVLSAVRSGLELPESRLPVYPKGERRRSGKSNWSPLSVQNLKEWRNRRAAALGLD
                     CSLVCTTSMIQALAARRPLTPEALDQVEGLRNWQKREFGAEICVVLRRGCPKK"
     gene            complement(4801..5970)
                     /locus_tag="PKN80_04265"
     CDS             complement(4801..5970)
                     /locus_tag="PKN80_04265"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HNQ35260.1"
                     /translation="MKSNRFYRLLALGMAGMLVAFGAACGGGGGSSSNSSGNLALYLT
                     DAFDETYQAVYVTIGEIQVSQVHEDDQTETDSWLTVARPRATYNLLELVNGVRQSLGI
                     AYLEPGRYSQMRLIIGTEPDNGTNIFSAAHPFANYVIDEAGHYHALKVPSGLQTGVKI
                     IQGFDISLNGTTELILDFDAARSVVKAGSSGQWLLKPTIKILNTEEYSIINGEVTDAD
                     ASEPAGGVVVSAQVYNPGAGDPKDAVTVRAATVTDAEGNYALFVQPGTYNLVAYLDDF
                     APTETGLVTQSGGNYSRSFEITGTDTGTVTGNVSIPGGDEDRYGVLSFRQTVTIDGSE
                     TQVEIKSVSVGGGGTYTVILPEGSYSLVASSYGLTTRVYDIDINQGSQTVRNVAF"
     gene            6422..6907
                     /locus_tag="PKN80_04270"
     CDS             6422..6907
                     /locus_tag="PKN80_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNQ35261.1"
                     /translation="MKPEKPLKFAVSMAACLAAGAIGSIFTASSVGGWYRTIEKPPFN
                     PPSWLFAPVWTTLYLMMGVSLFLAWSSRGRPAAEKDPALVVFAVQLGLNILWSAFFFG
                     LENPGLAFLEILILWAAILATIILFRKLSSLAAGLLLPYLLWVTFASVLNGSIFFLNL
                     P"
     gene            6972..7727
                     /locus_tag="PKN80_04275"
     CDS             6972..7727
                     /locus_tag="PKN80_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNQ35262.1"
                     /translation="MKEANYRNFLKDAIRKELDFSGTEQNRGLPPPPVEKPFPAGGRV
                     IALPGPDAWPNLGSLTVREAIEGRKSVRNYSRAPLALDELAFLLWATQGIRSRVIKGH
                     AFRTVPSAGCRHSFETYLAVFRVAGLEKGLYRYLPLSHSLLCLESVKDLETAAAEAAL
                     GQGFVGAGAVTFIWTTLPARMEWRYGPASYKVIALDAGHVCQNLYLACRAVGAGTCAI
                     AAYDQEKADALVGADGREEFVIYLAPVGKLPER"
     gene            complement(7734..>8106)
                     /gene="msrA"
                     /locus_tag="PKN80_04280"
     CDS             complement(7734..>8106)
                     /gene="msrA"
                     /locus_tag="PKN80_04280"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNQ35263.1"
                     /translation="ENPSYEQVCAGDTGHYEAVEVTFDPDRTGYEELLKLFFQVHDFT
                     QADGQGPDIGPQYRSAVFYASEGQKETAERVIGELKTRGYRVTTSIRPLSVFWKAEEY
                     HQDYYGRNGRRPYCHNFRRIF"
BASE COUNT         1338 a         2435 c         2579 g         1754 t
ORIGIN      
        1 tggggtgcca gctgtggggg gcgcagatcc actcttggtc atccccgagt tcgtccggga
       61 tgccctccac cagtaactgc ttaccgctgc cgtcggtccg catgatccac aggtcgctgg
      121 tctccgggtc caggccccag ttgcggcggt gataggagat tcagttgccg tccgggctcc
      181 agtttgccca gttctcgcag aaatcgccca cctcttccga gagcattacc aggccggtgc
      241 cgtcccgccg gaccttgtaa atccgggagt tggaattttc gctggggtcc gcgctgaaga
      301 gcacccactg cgagtccggg ctccagctgt gcttgttgcc tttgttgata tttccggaaa
      361 ccttgatcgg gtcggtcccg tcaatgtcca tcacccagat ttcttccgtg tcgcgatcca
      421 cgcaggcgcg gaaggctatt ttggttccgt ccgggctgac catcggtttc tgttcacact
      481 cgtttccatt gacgggtccg ccgtggaccg cggtcaggtt ctgctggtcg gtgccgtccc
      541 ggtccatgat ccagattctt gcataatctt ccgaatcgtc gtacctttga aagaggatcc
      601 ggttgccgtc gggcgtgaag ttgccgtggt tgtcgcaggt gttggtgttg ttggtaagcc
      661 gctgccagcc ggttccgtcc ggccggatga tgcagatttc gttgcgctgg tactcggaag
      721 cgccggttcc gatggccgcc tggtaaacga tccattcccc gtcgtaactc cagggattga
      781 cgccccagca ggggacccag tccttggcgc tctgggtcgg atttaaagga tcgcccggcg
      841 tgatctggac gatgctttcc accacgtcgt cgtcgaagac gccgaggagg ccgtggttga
      901 tggacgcctg ggcggtgagg gtgcccagca gcaggaaggc caggacaagc aagcaccatt
      961 ttttatgttt cattatcgct ctcccgtttt taaccgcgcg gatttaaatc tggctttcaa
     1021 gcagggtcaa acctgttatg ccatggccct cctaagcccg gaccgggctc gtcaattgtt
     1081 aggtgatcct gaacttgatt gagcaggcgg tagcttgaaa tttcagggga atcccttctg
     1141 aaaaaagaat ttcaatcgca acgggtacca caacttgttt tttttatacc gtaaataaga
     1201 atcattgtca agtaaagaga gcagtggtct gattgtttgc tgtaccattg aaagtaagaa
     1261 tttcttcatg ctcgctgctc acgaccaggc ggcgctcgtc ggccaggttg aagtagtaaa
     1321 ccgtggcgcc tttcgggagg gggccttcgg cgcggccggc ggccgcgtcc agttcggccg
     1381 gaatcgtttg ccacttgcgt tcccgccagg ggccggcgtc ggtcgtgtag ttgagttcgg
     1441 ccttgacgac cgggacccgg ccttcgaagg agacgaaggc ccggcgcccc tcccgtccgg
     1501 cggcggttat ccgcgccagg tcgattccgc ctttcagccg ggcttcggcc cgggtccgga
     1561 tctcctccgg attctcgccc ggtccgccgt gcccgtgggt catgcccacc ctgacacaca
     1621 gcgtccgggg cgatggcggc agccggtagg atttctgcag cgagtccagc ggataggcgg
     1681 cgtcggttgg gccggtgacc cagagcaggg gtgtctttac aagcggcaga tagaccgagg
     1741 gatcccagag ccggagccag cggccggatt tttccgttcc catccttcgg aaatcgtcca
     1801 gccagatcga gttgtcgccg aggaagccgc agccgtaaac cggggcggcg aacttgaagc
     1861 gcgggtcaac cccggccgcg atgcaggtca ggtagccgcc ccaggagatg ccggtaaggc
     1921 cggtcttgtt cgggtcaacc tccggaaagg agcgtatcag ggaatgggcg aggatgacgt
     1981 cggcgaccgc gtggcaggtc cactgatctt cgatcggttg gtcaacctgg tcgaagccgc
     2041 cccagccggg cgggccgcct ctcgggtggc gcgggtggtt ctggtagccg ccgccgctga
     2101 cgcagccgca ggtgtccatg gcgatcgccg cgtaaccgcg gccggcccag agacgcaccc
     2161 agggaacaaa ggccgaaccg ccgcccccgt gcaccaggac catggccggg atttttgact
     2221 tccccccggc cctggggatg gcgtaatagg cgaagacgcc ggtgcctttc ccgcgccagg
     2281 gcaggccctg gtagaagatg gcctttacct cgccatcgtc gtgttcgggg gccgcctcga
     2341 attcgggcgc ggcggaaaga ttcttgatat cccagggtat ccggcttggg ccgggttggc
     2401 cgtcggatga tccggtcggc aggcgtctct tcatttattt caggatgatt tccgccattt
     2461 ttaccacttc caggtccgag cccttcaccc ttttcagggt ggcgagttcc tcgtcgttcc
     2521 agcgcgggtc gggggcgccg gttatgaacc agtcgccgcc gttgtccgcc aggatcatcc
     2581 cgtatttctt gagcgcttcc aggataatcc gggcggcggc cggaaagccg ctggtgtcat
     2641 aatttttttt aaggcgaacc cgcatgccca tgggcggcag gccggggtcg gtcagccggc
     2701 tggcaaaatg acgggcgggg aagacatagg cccgtctcgt ccgggtgcag gtgaacctaa
     2761 gggcgtggcg tatttcaccc gtttccatga cctcgtcata ccggaccagc ccggggaaga
     2821 ttggcagccc ggcggcatcc gccgatgtcc agccggccgg acgcagcgcg ttggaggtga
     2881 ggtcgaagat ggccccggag ccggcccgcc atcgtttgcc gccctcctcc gggtaggccg
     2941 cgtacaattc gtaaagtttc cggttgtccc ggtcgagcat caggaggtgc cggtctccgg
     3001 aagccgtcgg cccgccctcg atcgggggat tggccgggat cgggtagggg gcggaatcgc
     3061 tttcggccgc gtattcgaag gagacgggca ccttcggctg ggtaccgggg acgatgacgt
     3121 aaggaatgcc gatgggagcg ttattccaga cggttccgaa atcgggatgg agtcccttgt
     3181 tcaggccgat ggaagcgatt agggccgcgg aattcgggtc ggccggcagt ccggagacat
     3241 cctggttcca ggggttgtcc accgggaaag gccggtatcc tttaagcgag gcgtttactc
     3301 ccaggagcgg ccggacgtcc ccggcggcgg ccgggaatgg cccgttcagg atcagccagc
     3361 cggtaaagag taatcggaga atgacgcttc ttctcatcca gcctcccggt cccaggtgaa
     3421 aatggccgtc attttttcgg gcagccccgg cgcaagacca cgcagatttc ggcgccgaac
     3481 tcccgcttct gccagttgcg aagtccttcg acctggtcca gggcctccgg ggtaagcggc
     3541 cgcctggccg ccagggcctg gatcatgctg gtggtgcaga ccaggctgca gtccagcccg
     3601 agggccgcgg cccggcggtt ccgccattcc ttcaggttct gcacgctgag cggcgaccag
     3661 ttgcttttgc cggatcggcg ccgttcgcct ttcgggtaga ccggcagccg cgattcgggt
     3721 aattccaggc cgcttcttac cgccgagaga acggccgccc ggaacttttc gctccgccgg
     3781 cccaggcctt tgatgcggag cagggcctgg tccgtctccg gcctctgctg gatgatctgg
     3841 cgtatcgcgt cggggtccag caccttgaag ggggggcggt ccctttcctg agccagggac
     3901 tcgcgcatct gcaggatagc ctcgagaatg gccaggctcc tgcgatccat gaccgcggcc
     3961 ccccggaacc ggaggtggag cggtccgttc tcgcgtcgcc ggtccggcgc ccgggccagc
     4021 aggtcgcatt cctcctcgac ccagtccagg cggccgagtc gtttcaggtc ttctttcatc
     4081 atctccgcca gccggatcag ccagtgaacg tcggcggcgg cgtaggcgag catctcgtcc
     4141 ggcagcggcc gctgcgacca gtcatccttc tggaatttct tgtcgagtga gacaccgaag
     4201 cgcttggcga ggacgtcggc cagtccggag cgggccatgc cgaggaagcg ggcggccagc
     4261 tcggtgtcga agatgggcgg gcagtttatt cccagccggc ctcttatcag ccggatgtca
     4321 taatccgaac cgtgcaggat cttttcgaca cccgtattcc caagtatggc cgagaggggg
     4381 gagaggtcct tgatcttgag gggatcgatc agccaggtcc ggctgccgtc cgagacctgg
     4441 gccaggcaga gggtgtctcc gtaatgaaac atggagtctc cctcgaggtc aagggcgatg
     4501 cttctggcac cggccagaac ctgggccgcc tttttaagtt cttcatgcgt ttccaccatc
     4561 tggtacttca ttttgcctgg caggtctggc atcggatctc cgttcgattc gggtaagcag
     4621 gatttaagtt tttaatccgg gttggccggt cttcgccggg aaggaccggg gcggtatcca
     4681 actgaatacg cccccgtctt tttgcgaagg ctgttttgaa agatacccgg cgtcggcctc
     4741 tgggccgacg ccggatcgta ttgtgccggt ttcgattgac tttccgtgaa ttttttgacg
     4801 tcaaaaagcg acattcctta ccgtttggga gccctggttt atgtctatgt cgtagacgcg
     4861 ggtggttaat ccgtaacttg aggcgaccag cgaatagctt ccttccggca ggataacggt
     4921 gtacgtcccg cctccgccga cgctgacgga tttgatttcg acctgggttt ccgacccgtc
     4981 aatggtgacc gtctgcctga aactgagcac gccataccgg tcttcatcgc cacccgggat
     5041 ggatacgttc ccggtgaccg tgccggtgtc ggtacccgtg atctcgaaac tccgggagta
     5101 gtttccgccg gactgggtga cgagacccgt ctccgtcggg gcgaaatcgt cgagataggc
     5161 caccaggttg taagtcccgg gctggacgaa gagggcgtaa ttgccttcgg catcggttac
     5221 cgttgccgcc cggacggtca ccgcgtcttt cgggtcgccg gcgccggggt tgtagacctg
     5281 ggcgcttacg accacgccgc cggccggttc gctggcgtcg gcatcggtaa cttcgccgtt
     5341 gatgatggag tattcctcgg tgttaagtat tttgatcgtg ggcttcagaa gccactggcc
     5401 gctgctgcct gctttgacca ccgaccgggc ggcatcgaaa tcgaggatga gttcggtggt
     5461 tccgttcagg ctgatgtcga agccctggat gatcttgacg ccggtctgca ggccgcttgg
     5521 gaccttcaac gcgtggtaat ggccggcctc gtcgattaca tagttggcga aggggtgggc
     5581 ggcgctgaaa atattggtgc cgttgtccgg ttcggtcccg atgatcaagc gcatctgcga
     5641 gtaacggccc ggctccaggt aggcgatgcc gagcgattgg cgcactccgt taaccagttc
     5701 gagcaggtta taggtggcgc gcggcctggc cacggtcagc cagctgtccg tttcggtctg
     5761 gtcatcctcg tgcacctggc tgacctggat ttcgcctatg gtgacgtaga cggcctggta
     5821 ggtttcgtcg aaggcgtccg tcaggtaaag agccaggttt cccgaggagt tgctgctact
     5881 gccgccgccc ccgccgcagg cggctccgaa agcgactaac atcccggcca ttcccagcgc
     5941 cagcaatctg taaaagcggt tacttttcat gattcccgcc tccttctttc catcaagttt
     6001 gaaactcgtg cggacaaggt tgttgcccgg gggcttcgtc gcacgttcgt gccccttttt
     6061 actctattaa atcacttcaa tggcttggaa atcaaccctt ggtaaggaga agggtgcggc
     6121 gtcaacccca tttcccgggt ctcgtgatat tcgttccggg ccgaatcctg ggaattcatg
     6181 ggaaaaagag gcgggaaatg tgatctttct cggggccccg gtcaacctcg ttttgaaaga
     6241 tgcgaaaccg ctgatttgct gggccaacag cggccgccag ggtcaggatg cgcttttttg
     6301 ccttcggccg ctccccccgg ttacaatatg taaaataatg gccgggagca accgggttga
     6361 ttcccattct ccgggatggc tggtctccgg ttgcctttag cgaaggagag cggagaaact
     6421 tatgaaacca gaaaagccct tgaagtttgc cgtttcaatg gcggcctgcc tggcggccgg
     6481 agcgatcggt tccatcttca ccgcctcttc cgtgggcggc tggtaccgga cgatcgagaa
     6541 gccccctttc aacccgccgt cctggttgtt cgcgccggtc tggaccaccc tgtacctcat
     6601 gatgggcgtc tccctgttcc tggcctggtc ttcccggggg cgtccggcgg cggaaaagga
     6661 tccggccctg gtcgttttcg cggtgcagct gggtctcaat atcctctggt cggccttctt
     6721 cttcggcctg gagaatcccg ggctggcctt cctggagata ctgattctct gggcggcgat
     6781 cctggccacg atcatcctgt tcaggaagtt gtcgtccctg gcggccggcc tgctgctgcc
     6841 ttacttgttg tgggttacct tcgcctccgt cctgaacggt tcgatcttct tcctgaatct
     6901 tccttgaaac ggaagttccg ggcggcaatt taatggtgtt tcggccggcc aaccggccaa
     6961 gggaagggga aatgaaagag gccaactacc ggaacttcct gaaggacgcc atccgcaagg
     7021 agcttgattt ttcaggaacc gaacagaacc gggggctgcc gccgccgccc gttgaaaagc
     7081 cctttccggc cggcggccgg gtaatcgcgc tgccggggcc cgatgcctgg cctaacctgg
     7141 gaagcctgac cgtccgggag gccatcgagg ggcggaaatc cgtccgcaat tacagccggg
     7201 ccccgctggc cctggacgaa ctcgcctttc ttctctgggc cacccaggga atccgcagcc
     7261 gggtgatcaa agggcatgcc tttcggacgg ttccctcggc cggctgccgc cactccttcg
     7321 agacctacct ggccgtcttt cgcgtggccg ggctcgaaaa aggcctttac cgttacctgc
     7381 ctctaagcca ttccctgctc tgcctggagt cggtcaagga cctcgagacc gccgcggccg
     7441 aagcggccct tggccaggga ttcgtcggcg ccggtgccgt caccttcatc tggacgacgc
     7501 tccccgcgcg catggagtgg cgctacgggc cggcctccta caaggtgatt gcgctggatg
     7561 ccggccatgt ctgccagaat ctttacctgg cctgccgggc ggtgggggcc gggacctgtg
     7621 ccatcgccgc ctacgaccag gagaaggccg atgcgctggt cggagcggac ggccgggagg
     7681 agttcgtcat ctacctggcc ccggtcggca agctgcccga gcgctaaggc gcctcagaag
     7741 atccgccgga agttgtggca gtaagggcgc cgtccgttcc tgccgtagta atcctggtgg
     7801 tattcctccg ccttccagaa gacggagagc ggccggatgc tggtcgtcac ccggtaacct
     7861 cgagtcttca attccccgat gactctttcc gccgtttctt tctgcccttc cgaggcgtag
     7921 aagaccgccg atcggtactg gggtccaatg tcaggtccct ggccgtcggc ctgagtgaaa
     7981 tcgtgcacct ggaagaataa tttcaggagc tcttcgtaac cggtccggtc gggatcgaag
     8041 gtcacctcga ccgcctcgta gtggccggtg tccccggcgc aaacttgttc gtaagacggg
     8101 ttttcg
//
LOCUS       DAOQYN010000077         7956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9557_length_7956_cov_4.133401, whole genome shotgun sequence.
ACCESSION   DAOQYN010000077 DAOQYN010000000
VERSION     DAOQYN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9557_length_7956_cov_4.133401"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..3975)
                     /locus_tag="PKN80_04285"
     CDS             complement(4..3975)
                     /locus_tag="PKN80_04285"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNQ35264.1"
                     /translation="MSRKNNPVGYAAPSAIAFKFFGSVVSLLFTLCFSLLLPAKPAAG
                     SIGEDLKYLGSLESRLAGSPGSRAASDYIEARFRALGMQDISREKVNVTVPVDEGSYL
                     QQGGRRITIHPLWPNLVRTSMVPPEGLSGRLVFGGSGQLENFLGQDLKDSIVILDFNC
                     AYRWLDATTLGARAVIFVEPEETSVFEARRKFLSLALDVPRFYMSRAEAVSLAAAARS
                     NPIATVYCRQRWERRETYNLIGYLKGTDPELSKQVICLNAYYDSMSVVPELAPGAGQA
                     VGVAALLDLAAYFSANPPKRTLLFLANGAHHQGLRGMVDFVHRHSQKPPKGSKIPEEL
                     KNQQPIPIKLYINLDLSGHSDRVCLYHNTTDFSAQRYFAPFAKKLFGYLTPAQKDKLY
                     NGISPEKGLSYSTFMPESLISDGLVPIVAGIPSLSFVTIYDSRRYFDTPMDRPERIDY
                     TLVDNQVSMLRNLLGRALGDPDLFPSNELTFKDKLYFLNGRLVNFNPQKSFVPNDPVP
                     GALACVLNAGPKSNYGLRCQVVEMTDDQGSFLVAPLGWTRMPYTLEGYVTDPENGRIV
                     MAPDRGVNGAENYPIEFTMDTPVKEWRAVIFRCEGYNLFDFVDPRYLMQLTKVDIFNP
                     SNAIPYAYGYSLPERDTKEIKWTSEVEPAAVVFTEPGMPLKVVGEAGLLGKRVLLINA
                     PESSEKLLSEGVGYEAAGDPNILNLTPYRAARDMINLNEFRINNFRRFGVNNERLTAL
                     HQQAREWFDKATEDLARFDWFSFLTNSRRAVGMESRAYPDVRSTTNDVIKGLIFYFVI
                     LLPFAFFSERLFFGFVDIRKQIAGVFAIFLVIFVIMRFAHPGFRMSNSPEVILLAFVI
                     LTLAGIVIFLISGKFEEQMQRLKMERAKVYETDVGRMTTIGTAFALGIANMKRRKMRT
                     SLTAVTLILLTFTVLSFTSIKNYLQFNQIAKNYPASYEGMMMRDRTWSPLEGPAYEYV
                     RNEFSPKAVVAPRAWLLNPLMENKTFILVTAGGRESFASGLLGLTAQEKEITGLDRFL
                     TAGSWFEANDEKTVLISRSMAGLLDFPASEVGRGVVRIGGEELVVRGIFDESKLNAFR
                     DLDGEIITPVDFTVMPVQELGKLKSDPTAKALTSASVSESFSHQEAGSVILMPFERLY
                     RGGGTIQSLAIKFQPGFDVRREIEGFVSRLAVTVFTGLGGKTFAYSSLGLTSMSGIGN
                     LFIPILIASLIVLNTMLGAVYERIREIGIYSSVGLAPSHIAALFFAEACVYAIIGAVA
                     GYLLGQVVAKILMAAGLLRGLTLNYSSLSAVSTTLIVMVVVFLSTIFPAFMTEGSNWT
                     RTL"
     gene            complement(3959..4516)
                     /locus_tag="PKN80_04290"
     CDS             complement(3959..4516)
                     /locus_tag="PKN80_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35265.1"
                     /translation="MTPGFKRILLVVGALIWVGVLLAYRGPGGNRGKALRLEREGERL
                     YRQGQVETAVGLWQQAIELAPERVFAYEKLSAHYLLRGEWQKAQSLASQGLTHSPRCV
                     NLYFNQGLSLFQSGEFKAAADSIDRVLASDDYYPNAHYLKGLIYHALGESEQARAEFV
                     KELNVNPANPGAWKAIQEMNHVKKK"
     gene            complement(4524..4739)
                     /locus_tag="PKN80_04295"
     CDS             complement(4524..4739)
                     /locus_tag="PKN80_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35266.1"
                     /translation="MDPEKKLRLAIGIENGLILLAIVALWPVILGWPHRYSQPLLLLA
                     VAVMGAVAWRRWRRLLAAMKEARQNNG"
     gene            complement(4757..6190)
                     /gene="lysS"
                     /locus_tag="PKN80_04300"
     CDS             complement(4757..6190)
                     /gene="lysS"
                     /locus_tag="PKN80_04300"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNQ35267.1"
                     /translation="MAEEDILAERRKKLAGLLERGVEAYGRRYDRTNPIAAVTESSER
                     VLICGRLMAWRDHGKAAFADLEDFSGRIQLYFQRDRLGDDFSRVKDLDIGDFVGVAGR
                     PFRTRTGMLTIGVEEFTLLSKSLRPLPEKWHGLKDVEIRYRQRYLDLLANPDTRKVFA
                     TRSRIIAGIRRQLDQLGFLEVETPMMHPIPGGAEARPFVTHHNALELELYLRIAPELY
                     LKRLLVGGLERVYEINRSFRNEGISTLHNPEFTMLELYAAYGDYRLMAELAELLILNA
                     IDELFPDSGRRLSYQGREIDFTPPWPRLTLAELLRERLDLPGWDDRSLLLERARAGGL
                     ELDGSETVFDLLDLLFKFRIQPGLVRPTFVLDYPAEISPLAKSKPGQPGVVERFELFI
                     GGLEVGNAYSELNDPIDQERRFQAEVRTGRPDRPKVVDEDYIEALSYGMPPAGGLGIG
                     LDRLVMILVDTASIRDVILFPILRPVR"
     gene            6490..7443
                     /locus_tag="PKN80_04305"
     CDS             6490..7443
                     /locus_tag="PKN80_04305"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNQ35268.1"
                     /translation="MPHPLIDQESAEQVEKSVRRTIQKSDVSNLVTTVVLLIIVLAAV
                     SGIVLLSLKISRTSRIASSNAGGTGGQKPEIVRPTPTAPLPLPGGSGPGSAPPPAPGF
                     GSGNPSGPVPENMVLVGGGEFIKGYDSKGQPVRFSLPPFYIDQHEITNREYLVFIKAT
                     GRRPPSDPRGVSFNIWKNGTFAPELEDHPVVNVSFADAEAYAAWAGKRLPNENEWEKA
                     ARGVQGYIYPWGNNFNPELANCAENPRYGTGTAPVGSFNSGRSPYGVLDMAGNVKEWT
                     TTSVSSRNSRWKVVKGGSFRDRAGQLETISSQRGILAAPDL"
     gene            complement(7525..>7956)
                     /locus_tag="PKN80_04310"
     CDS             complement(7525..>7956)
                     /locus_tag="PKN80_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HNQ35269.1"
                     /translation="GAAAVRPAAFLELPVDILVPAALEEVITGANAARIRPRILAELA
                     NGPTTPEADHILEKNGVHVIPDFLCNAGGVTVSYFEMVQNAYLYAWDQLRIEERLDRK
                     MTTAYHEVLATARRYNLGMRQAAYQVAVQRVIEAMRLRGWI"
BASE COUNT         1668 a         2377 c         2426 g         1485 t
ORIGIN      
        1 cgtctacaag gtcctcgtcc agttcgagcc ctcggtcatg aaggccggga agatcgtgga
       61 aagaaaaacc accaccatca cgatcagggt cgtgctgacc gccgaaaggg aggagtaatt
      121 gagcgtcaat cctctcagca ggccggcggc catcagtatc ttggccacca cctggcccag
      181 caggtaaccg gccaccgccc cgatgatggc gtaaacgcag gcctcagcga aaaagagggc
      241 cgcgatatga ctgggagcca gcccgacgct actgtaaatg ccgatctccc ggatccgctc
      301 gtacacagct ccgagcatgg tgttaagcac aatcaggctg gcgatcagaa tcggtatgaa
      361 gaggttcccg atcccgctca tggaagtcaa gccgagagag gagtaggcaa aggtcttgcc
      421 gcccaggccg gtgaagacgg tcaccgccag ccgggaaacg aaaccttcga tctcccggcg
      481 aacgtcaaag ccgggctgga acttgatggc cagggattgg atggttccgc ccccccggta
      541 aagacgctca aagggcatga ggatgacgct tccggcttct tggtggctga agctctctga
      601 gacgctggcg gaggtcagcg ccttggcggt cgggtcgctc ttgagtttcc ccagttcctg
      661 caccggcatc acggtgaaat ccaccggggt gatgatttcg ccatccaggt cgcggaaagc
      721 attgagtttc gattcgtcaa agatgccgcg taccaccagt tcctcgccgc cgatccggac
      781 aacgccccgg ccgacttcgg acgccggaaa atcgagcagc ccggccatcg acctggaaat
      841 cagaaccgtt ttttcatcgt tcgcttcgaa ccaggaaccg gccgtcagga aacggtcaag
      901 cccggtgatc tccttctcct gggcggtaag acccagcagg ccggaggcga agctttcgcg
      961 gccgccggcg gtaaccagga taaaggtctt gttctccatc agcgggttga gcagccaggc
     1021 gcgcggggcc accaccgcct tgggtgaaaa ttcgtttcga acgtactcgt aagccggccc
     1081 ttccagcggc gaccaggtgc ggtcgcgcat catcatcccc tcgtaagagg ccgggtaatt
     1141 cttggcgatc tgattgaact gcaggtaatt cttgatcgag gtgaaggaaa gcaccgtgaa
     1201 agtcagcaga atcagggtga cggccgtcaa ggaagtccgc atcttgcggc gcttcatgtt
     1261 cgcaatgccc agggcaaagg cggtaccgat ggtggtcatc cggccgacgt cggtctcata
     1321 aaccttggcc cgttccatct tgagccgctg catctgctct tcaaacttgc cggaaatcag
     1381 gaagataacg atgccggcca gggtaaggat gacaaaggcc agcaggatga cttccggact
     1441 gttactcatc cgaaaaccgg gatgggcgaa gcgcatgatg acgaagatga ccaggaagat
     1501 ggcgaaaacc ccggcaatct gtttccggat gtccacgaaa ccaaagaaga gacgctcgct
     1561 gaagaaggca aacggcagca ggatcacgaa ataaaaaatg aggcctttga taacgtcgtt
     1621 ggtggtggac cggacgtccg gataggcccg ggattccatg ccaaccgccc ggcggctgtt
     1681 ggtgagaaag gagaaccagt cgaaccgggc caggtcctcg gtggccttgt cgaaccattc
     1741 ccgggcctgc tggtgcaacg cggtaagcct ttcgttgttg accccgaaac gccggaagtt
     1801 gttaatgcgg aactcgttga ggttgatcat gtcccgggcg gcccggtaag gggtcaggtt
     1861 caggatgttc gggtcgccgg ccgcctcgta ccccactccc tcggagagca gtttttcgga
     1921 agactcgggc gcattgatga gaagcacccg ttttccgagc agaccggctt cgccgaccac
     1981 cttgagcggc atgcccggct cggtgaaaac aaccgccgcc ggctcgactt cggaagtcca
     2041 cttgatttcc ttggtatccc gttccggcag cgagtacccg taggcgtacg gaatggcgtt
     2101 ggaaggattg aagatgtcaa ccttggtcag ctgcatcagg tagcgcggat cgacgaagtc
     2161 aaacaaattg tagccttcgc agcggaagat gaccgcccgc cattccttga ccggggtatc
     2221 catggtaaac tcgatcgggt aattctcggc gccgttcacg ccccggtccg gggccatcac
     2281 gatccggccg ttctccggat cggtcacgta tccttccagc gtatagggca tccgcgtcca
     2341 accaagcgga gcgaccagaa atgaaccctg gtcatcggtc atttccacca cctggcagcg
     2401 caggccgtaa ttgctcttcg gcccggcgtt cagcacgcag gccagggcgc cgggcaccgg
     2461 gtcgttcggc acgaagctct tctggggatt gaaattgacc aggcgcccgt tgaggaaata
     2521 aagtttgtcc ttgaaggtga gctcgttgga cggaaagagg tccggatcgc ccagggcccg
     2581 ccccagcagg ttgcgcagca tcgacacctg gttgtcgacc agggtgtaat caatccgctc
     2641 gggacggtcc atgggcgtgt caaaataacg ccggctgtcg tagatggtga cgaaggaaag
     2701 ggaggggatt ccggccacga tgggaaccag gccgtcgctg atgagcgatt ccggcatgaa
     2761 ggtcgaataa ctcaggcctt tctcggggct gatgccgttg taaagcttat ccttctgggc
     2821 gggcgtcagg tagccgaaaa gttttttggc gaagggggcg aagtagcgct gggcgctgaa
     2881 atcggtcgta ttgtggtaaa gacagacccg gtccgagtga ccggaaagat cgaggttgat
     2941 gtagagcttg atcggaatgg gctgctgatt cttcagctcc tcgggaatct tgctcccctt
     3001 gggaggcttc tgactgtgcc ggtgcacgaa atccaccatg ccgcgcaagc cctgatgatg
     3061 ggctccgttg gccaggaaga gcagcgtgcg cttgggcggg ttggctgaaa aataggcggc
     3121 caggtccagc agggcggcga cacccaccgc ctgaccggcg ccgggagcca gttcgggaac
     3181 caccgacatc gagtcgtaat aggcgttgag acagatgacc tgcttggaga gttccggatc
     3241 ggtgcctttc aggtaaccga tcaggttgta cgtttcgcgc ctttcccagc gctgccggca
     3301 gtaaacggtg gcgatcgggt tgcttcgggc ggccgccgcc agcgaaaccg cttccgcccg
     3361 ggacatgtaa aaccggggca catccagggc caggctcagg aatttgcgcc gggcttcaaa
     3421 aaccgaggtt tcttccggct ccacgaaaat tacggcccgg gcacccaggg tggtggcgtc
     3481 cagccaccgg taagcgcagt tgaaatccag gatgacgatg gaatccttga ggtcctgccc
     3541 cagaaaattc tccagctgcc cgcttccgcc gaagaccagg cggccgctga gcccttcggg
     3601 cggaaccatc gaggttcgca ccaggttcgg ccacaggggg tgtatcgtga tccggcggcc
     3661 gccctgctga agataggagc cctcgtcaac cggaacggtc acgttcacct tctcccggga
     3721 aatatcctgc attcccaggg cccggaagcg ggcctcgata taatcggagg ccgcccggct
     3781 gcccggggat ccggccaggc gcgattccag ggaaccgagg tatttcaggt cctcgccgat
     3841 ggagccggcg gccggcttgg ccggcagcag caggctgaaa cagagggtga acaggaggga
     3901 aactaccgaa ccgaaaaatt tgaaggcgat ggcggacggg gcggcataac cgactggatt
     3961 attttttctt gacatggttc atttcctgaa tcgctttcca ggcaccgggg ttggctgggt
     4021 tgacattcag ttctttgacg aactcggctc gggcctgttc agactcgccg agtgcatgat
     4081 aaatcagtcc tttcaggtag tgggcgttcg gatagtagtc gtctgaggcc aggacccggt
     4141 caatgctgtc ggcggccgcc ttgaactctc cgctctgaaa caaagacaag ccctgattga
     4201 agtaaagatt aacacatcgc ggtgaatgtg tcaaaccttg actggcaagc gattgagcct
     4261 tttgccactc gccgcggagc aggtagtggg ctgagagttt ttcataggcg aagacccgct
     4321 ccggggccag ttcgattgcc tgctgccaga gtccgacggc cgtttcaacc tgcccctgcc
     4381 ggtagaggcg ttctccctcg cgctcaagcc ggagcgcctt gccccggttg ccgcccgggc
     4441 cacggtaagc cagcagcacg cccacccaga taagggcgcc gacgaccagc agaatccgtt
     4501 taaatccggg ggtcatgaat cagttacccg ttgttttgcc gcgcctcttt catggcggcc
     4561 agcagacggc gccagcgccg ccaggcgacc gcccccatga ccgcaacggc cagcagcaac
     4621 agcggctggc tgtaacggtg cggccagccg aggatcaccg gccagagcgc gacgatggca
     4681 agcagaatca gcccgttctc gattccgatg gccagccgga gtttcttttc aggatccacg
     4741 cggttctccc cgccggtcac cggaccggac gcagaatcgg aaagaggatc acgtcccgga
     4801 tggaggcggt gtcaaccagg atcatcacca accggtccag gccgatgcca agcccgcccg
     4861 ccggaggcat gccgtaggaa agggcctcga tataatcctc gtcaaccacc ttgggccggt
     4921 ccggccgccc ggttcggact tccgcctgga accggcgttc ctggtcgatc gggtcgttca
     4981 gttccgagta ggcgttcccc acctccaggc cgccgatgaa aagttcgaaa cgttcaacca
     5041 cgcccggctg ccccggcttg ctcttggcca gcggggatat ttccgccggg taatccagca
     5101 cgaaggtcgg ccggaccaga ccgggctgaa tccggaactt gaagaggaga tccagcaggt
     5161 cgaaaacggt ttcggagccg tccagctcaa gtccgccggc ccgggcccgt tccaggagga
     5221 gcgaccggtc atcccagccg ggcagatcga gcctttcgcg cagcagttcg gccagggtca
     5281 gcctcggcca gggaggcgta aaatcaatct cgcgcccctg gtaggaaagg cgccggccgc
     5341 tatccggaaa gagttcgtcg atcgcgttga gaatcagcag ttcggccagt tcagccatca
     5401 accggtaatc gccgtaggcg gcgtagagct ccaacatggt aaattccggg ttgtgcaggg
     5461 tcgaaattcc ttcgttccga aaggaccggt taatctcgta aaccctttcc agtcccccga
     5521 ccagcagcct tttcaagtat agttccggag caatacgcag gtagagttcc aactccaggg
     5581 cgttatggtg ggtaacgaaa ggacgggctt cggcgccgcc cggaatggga tgcatcatcg
     5641 gcgtctccac ctccaggaag ccgagttggt ccagctggcg ccggatgccg gcgatgatcc
     5701 ggctgcgggt ggcgaatact ttccgggtgt cagggttggc cagcaaatcc agatagcgct
     5761 ggcggtaacg gatctcgaca tccttgagcc cgtgccattt ttccggtagc ggccgcagtg
     5821 acttcgacag cagcgtgaac tcttccaccc cgatcgtgag catgccggtg cgggtgcgga
     5881 acggccggcc cgccactccg acgaaatccc cgatatcaag atccttcacc cggctgaaat
     5941 catcgcccag ccgatcccgc tggaaataaa gctgaatccg gcccgaaaaa tcctcgagat
     6001 cggcaaaggc ggccttgccg tggtcgcgcc aggccatcag gcgcccgcag atgaggactc
     6061 tttcagagga ctcggtcacc gccgcgatcg gattggtccg gtcgtaacgg cggccgtagg
     6121 cttcgacccc gcgttccagg agcccggcca atttcttgcg acgctccgcc agaatatctt
     6181 cttcagccat tttcaccctt ttcaaacgcc gtcccgaacc gggcattcac ctgacggaga
     6241 cggtttgtaa tttgaaaatt ttaattacta tagattttat actttagccg gattcaaaat
     6301 caacacttgt cttttttttg atttttcggt aaaataatct tgatattttt ccaaaagcgt
     6361 ggccaacccg agtaaaaagg acgggcggac gggatgggaa cgggccgccc ggaagagccg
     6421 ccttcacatc aagggccggt aaaagggtga ttttttgctt tcgccggcgg caaccgcatt
     6481 agaggttaaa tgccacaccc gttgatagac caggaatcgg ccgaacaggt tgaaaagtcg
     6541 gttcgacgca ccatccagaa atcggacgtt tcgaatctgg tgacgacggt agtactgttg
     6601 ataatcgtgc tggccgcggt gagcggcatc gttctgctca gcctcaagat cagccgcacc
     6661 tccaggatag cctccagcaa tgccggcggc accggcggac aaaagccgga aatagtgcgt
     6721 ccaaccccga ccgcaccgct gcccctgccc ggcggaagcg gtcccggaag cgcaccaccg
     6781 ccggccccgg gcttcggttc cggaaatcca agcggcccgg tcccggaaaa catggtcctg
     6841 gtcggcggcg gtgaattcat caagggatac gactccaagg gtcagccggt gcgtttctcg
     6901 ctgccccctt tctacatcga ccagcacgaa attaccaacc gcgagtacct ggtcttcatc
     6961 aaggcgaccg gccgccggcc gccgtccgat ccgcgcggcg tcagtttcaa catctggaag
     7021 aacggaacct tcgcgccgga actcgaggat cacccggtgg tcaacgtaag cttcgccgac
     7081 gccgaagctt acgccgcctg ggccggcaaa cggctgccga acgaaaacga atgggagaag
     7141 gccgcccggg gtgttcaggg ttacatctat ccctggggca acaattttaa cccggaactg
     7201 gccaactgcg ccgaaaatcc gcgttacgga accggtacgg cgccggtcgg cagcttcaac
     7261 tcgggacgca gcccttacgg tgtcctggat atggccggaa acgtcaagga atggaccacc
     7321 acttcggttt ccagccgcaa cagtcgttgg aaggtggtca agggaggttc gttccgggat
     7381 cgggccggcc agctggaaac catttcatcc cagcggggaa tactggcggc ccccgatctc
     7441 tgattccgct gcgtcaagga cgcccagtag ccggccccgc cgtcaaaatc tctccgcccc
     7501 gtttcaaaag ccgccggcgg ctcgtcaaat ccaaccccgc aaccgcatcg cctcgataac
     7561 ccgctgcacc gccacctggt aagccgcctg gcgcatgccc aggttataac gccgggccgt
     7621 ggccagcacc tcgtggtagg cggtggtcat cttccggtcc agacgctcct cgatgcgcag
     7681 ctggtcccag gcataaagat aggcgttctg gaccatctca aagtaggaaa cggttacccc
     7741 gccggcgttg cataagaagt cgggaatgac atgcacgccg ttcttttcaa gaatatggtc
     7801 ggcctccggg gtggtcggtc cgttggccag ttcggccagg atgcgggggc ggatccgggc
     7861 ggcgttcgcg ccggtgatca cctcttcgag ggcggccgga accagaatgt cgaccggcag
     7921 ttccagaaag gccgccggcc ggaccgccgc ggcgcc
//
LOCUS       DAOQYN010000078         7899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9641_length_7899_cov_4.679628, whole genome shotgun sequence.
ACCESSION   DAOQYN010000078 DAOQYN010000000
VERSION     DAOQYN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9641_length_7899_cov_4.679628"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKN80_04315"
     CDS             complement(<1..564)
                     /locus_tag="PKN80_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNQ35270.1"
                     /translation="MRKRASLKVLLLLLFLAAGQAGQVRAQEPQVRLMRGGRVMTLGL
                     EDYLIGVLAGEVSPNWPLEALKTMAVCARTYTINKMMVNSGGNYHLTDTMMDQVYKGN
                     GRLWSSIRQAVDETRNEVLTYESAPAQVFYCASSGGCTASSLVVWGRYYPYLQAVADI
                     YSTSDPYANWTLKVSERDFLRALGYTGH"
     gene            complement(542..1156)
                     /locus_tag="PKN80_04320"
     CDS             complement(542..1156)
                     /locus_tag="PKN80_04320"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNQ35271.1"
                     /translation="MEKVNGRRRGFRTAAAVVLVAGVMLFLGRAGWRLRNQAPDLSVG
                     EAPSREEVTAFNYERSEPGLSLLVTGEAANVDSGSATEIRSPSVRLETGGKIIKIQTA
                     AGGTAELNLDPATGRADRIQLRKGVRISRQDSKTEQVEFVAQGEEATYDSGRQEILLT
                     GNPVISQGNNEFRGERIRYLLRENRILIENNASGIIHYEEKGKP"
     gene            complement(1143..2117)
                     /locus_tag="PKN80_04325"
     CDS             complement(1143..2117)
                     /locus_tag="PKN80_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNQ35272.1"
                     /translation="METLKRAREVFRSEIEAVEATERRLGRNFEAAVEKLAACSGRVV
                     ITGMGKSGIIGRKIAATLSSTGTPAFFLHPAEAVHGDLGVIARGDVVLAISNSGKTDE
                     LLRILPSIRKIGAVTIAITGDPDSPLAAAADLVLETRAVNEADRFDVIPTASTTALLV
                     LGDALAVCTLERKGFQRRDYAFLHPAGALGQQLTRLVDDLMRPASKMPRVSEGVPLSA
                     ALKTMSQGDLGLVVVTDADGLLAGILTDGDLRRHLIEKDSIKDFRVEDLMTRKPKTIK
                     AGSLAVNAVRIMEKHEITALIVVDRGNRPVGVIHLHGILGGKRLYGKG"
     gene            complement(2105..2926)
                     /gene="kdsA"
                     /locus_tag="PKN80_04330"
     CDS             complement(2105..2926)
                     /gene="kdsA"
                     /locus_tag="PKN80_04330"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNQ35273.1"
                     /translation="MIVKIGEIGLGDANPFCLIAGPCVIESEALILETARRLKEICFS
                     VRVPLVFKASYDKANRTSIKSFRGPGLEAGLRILRRVKEELSLPVLSDIHQPSDAAPA
                     AEVLDVLQIPAFLCRQTDLLLAAGRTGRPVNVKKGQFLAPEDTRHLVGKIESTGNRRV
                     LLTERGSCFGYHNLVVDFRGLEVMGAFAPVIFDATHSVQQPSAGETSGGCRQFVPLLV
                     RAALAAGCAGLFLEVHPDPERACSDGPNMVKLDDLPGLLAEAKAIDQVVKERWKR"
     gene            complement(2970..3317)
                     /locus_tag="PKN80_04335"
     CDS             complement(2970..3317)
                     /locus_tag="PKN80_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNQ35274.1"
                     /translation="MSSECLFCRLVKGEIPAQKVFEDEEILAFRDINPQAPIHILVIP
                     KRHLSSLADFGEADRELLGRLQLAVRRVAEEQVGPGQDYRLVLNSGESAGQSVFHLHY
                     HLLGGRTFRWPPG"
     gene            complement(3314..4834)
                     /gene="infB"
                     /locus_tag="PKN80_04340"
     CDS             complement(3314..4834)
                     /gene="infB"
                     /locus_tag="PKN80_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNQ35275.1"
                     /translation="MKKKDLKARPPIVTLMGHVDHGKTSLLDAIRKSNIAGGEFGQIT
                     QHIGAYTVSNEGRRITFLDTPGHEAFTNMRARGAQLTDIVILVISVEEGVMPQTVEAI
                     SHCQAAQVPIIVALNKVDRGRENIPRIKGELARHNLLSEDLGGQALFVETSAAKRTGI
                     GELLEAILLQSEMLELKADYACPAEGVVIETRMDRGTGANISLVVLNGILRNRDHFTV
                     GDTWGRVRAIFDDAGKPMKEVTPGLAARVIGLSQLPPVASRFKVVDSEKTAREFCETN
                     LARPEAGTVSTPITLEEFYQRLQAEGAKDLNIILKADVAGSMEAIAESLQRLNTEELK
                     VRILHSGIGPVGEADVLLAKTSGSIVLAFNVNIDPKARETVRQENVQVRQYRIIYDLL
                     DDVRKSLSGLLEPELKEVVTGRVQIKQIFKLSSGQLAIGCSVLEGKVNRGGKARLMRG
                     DKAVAEVIVASLRRFKENVREVTAGYECGLTLDGADEALPGDQLVIYRIEEITRSL"
     gene            complement(4837..5985)
                     /gene="nusA"
                     /locus_tag="PKN80_04345"
     CDS             complement(4837..5985)
                     /gene="nusA"
                     /locus_tag="PKN80_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNQ35276.1"
                     /translation="MQLNRELVAILEYLERERGLDRQTVLSAIEGGLLSVYRKKLKLD
                     NITELHIDPQTTEVYLVDETGKRLPAPDMPLGRIMAQSARQMIMQKIREAEKSQLYDE
                     FKGKEGTIVTGLVDHYEQGSLVVNLGRIEGVFPVSELTRDERHRRRGSAIRGIIIEVR
                     REIQGPKIVLSATHPDLVKDLFMQEVPEIREGLVEIKSVARVAGEACKVAVTSNDNRI
                     DPVGTCIGIRGTRIRAIMKELDGERVDVIRWHADPAELIAATLAPAKVAKVEISAKQK
                     FSRVLVPDDQLAVAIGRKGINVKLASQLTGYTVDVRSESEARAETLPAIATLPGVTEE
                     LARRLKEAGIVSLNDLLSTTPEDLAKIEGLDSRGAEELLENARLSSAA"
     gene            complement(6222..6572)
                     /gene="rplQ"
                     /locus_tag="PKN80_04350"
     CDS             complement(6222..6572)
                     /gene="rplQ"
                     /locus_tag="PKN80_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNQ35277.1"
                     /translation="MRHQRRKRVNLNRGRRIGVFTSLLKGLVDNGRVVASEARIRQVQ
                     RTAEKLITLAKAKDLAGQRRIISVLKDQELTRKLVNEIAPRYAERPGGYTRLLKLPPR
                     EGDGCPLALLVFLP"
     gene            complement(6574..7497)
                     /locus_tag="PKN80_04355"
     CDS             complement(6574..7497)
                     /locus_tag="PKN80_04355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNQ35278.1"
                     /translation="MEQFNYPNKIEWDEKSYRANYGKLIVEPLERGFGVTLGNSLRRV
                     LLSAIPGVAITSFKITGVSHEFSTIKGVTEDVTDIALNLKQVELKSNISELPKTFLVN
                     IAEKTELVAEDLFPGPEIKVLNPRLHIASLAAGTKLQIELTVNRGFGYVSEDRITAES
                     EEKEKKIGTIQLAAAFSPVTRVAYHTETARVGQRVDYERLILEIWTTGAIEPKQTVII
                     ARDILKQHLDQVGGGPARAEAPAAAGVSLEKLGLATRIRNVLKAAGIETDQALAGRTE
                     AELEAVEGLGEKSLEELKKFMAKKKLKLKEA"
     gene            complement(7515..7853)
                     /locus_tag="PKN80_04360"
     CDS             complement(7515..7853)
                     /locus_tag="PKN80_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNQ35279.1"
                     /translation="MDNILVVSGFIDSRRQARQLIYGGNVRVNGKRVSASSYLAKTAD
                     EVEFRPGLKIRPQIKEKVAASSGQPARVVPAWVAVDKEALKIKVLREPARSEVTLPVS
                     EGHIVNLYSR"
BASE COUNT         1408 a         2574 c         2383 g         1534 t
ORIGIN      
        1 gtggccggtg taaccgagcg ccctcagaaa atcccgttcc gataccttca gcgtccagtt
       61 ggcgtatgga tcgctggtgc tgtatatatc cgcgaccgcc tgcaggtaag ggtaataacg
      121 cccccagaca accagactgg aggcggtgca gccgccggaa ctggcacagt aaaacacctg
      181 ggccggggcc gattcgtaag tgagcacctc gttgcgcgtt tcatccaccg cctggcggat
      241 cgaagaccag aggcggccgt tgcccttgta aacctggtcc atcatcgtgt cggtgagatg
      301 gtaattgccg ccggagttga ccatcatctt gttgatcgta taggtgcggg cgcagacggc
      361 catcgtctta agcgcctcca gcggccagtt cggagagacc tcgccggcca gcacgccaat
      421 caggtaatct tcaagcccca gggtcatcac ccgtccgccg cgcataagac gcacctgcgg
      481 ctcctgggcc cggacctggc cggcctggcc ggccgccagg aagagcagca gcagcagaac
      541 cttaaggctt gcccttttcc tcataatgga tgatcccgga agcgttgttt tcgatcagga
      601 tgcggttctc gcgaagcagg tagcggatcc gctccccccg gaattcgttg ttgccctggg
      661 agataaccgg gttgccggtg agcagtatct cctggcggcc ggaatcgtag gttgcctcct
      721 cgccctgggc cacgaactcc acctgttcgg tcttgctgtc ctgccggctg atgcggacgc
      781 ccttgcgcaa ctggatgcgg tcggctctac ccgtagccgg atccaggttc aattcggcgg
      841 tgccgccggc ggcggtctgg atcttgatga tcttgccgcc ggtctccagc cggaccgagg
      901 gtgaccggat ttcggtggcg gaacccgaat cgacgttggc ggcctcgccg gtaaccagca
      961 ggctcaggcc cggttcggaa cgctcgtaat tgaaggcggt cacttcctcc cgggagggcg
     1021 cttcgccgac ggaaagatcc ggcgcctggt tccgcaggcg ccagccggcc cggcccagga
     1081 aaagcatgac tcccgccacc aggacaaccg cggccgcggt ccggaagccc cggcgtcgcc
     1141 cgttaacctt ttccataaag gcgctttcct cccaggatgc cgtgcaggtg aatgacgccc
     1201 accggccggt tcccgcggtc cacgacgatg agggccgtta tctcgtgctt ctccatgatt
     1261 cgaaccgcat tcaccgcgag actgccggcc ttgatggtct tgggcttccg ggtcatcagg
     1321 tcctccaccc ggaagtcctt gatcgaatcc ttttcaatca ggtggcgacg caggtcgccg
     1381 tcggtgagga taccggccag gagaccgtcg gcgtcggtaa ccaccaccag ccccaggtcg
     1441 ccctggctca tggtcttcaa cgccgccgac agcgggaccc cttccgaaac ccggggcatc
     1501 ttcgaagccg gccgcatcag gtcgtcgacc agacgggtca gctgctggcc gagtgcgccg
     1561 gccggatgca ggaaagcgta atcccggcgc tggaatcctt ttcgctcgag ggtgcagacc
     1621 gccagggcgt cgcccagcac cagcaaggcg gtagtgctgg cggtggggat cacgtcaaac
     1681 cggtcggctt cattgaccgc ccgggtctcc agcaccaggt cggcggcggc ggccagcggc
     1741 gaatccgggt cgccggtgat ggcaatcgtc accgcgccga tcttccggat cgagggcagg
     1801 atgcggagca gctcgtcggt cttgccgctg ttggagatgg ccagcacgac atccccgcgc
     1861 gcgatcacgc ccaggtcgcc gtgaaccgcc tcggccgggt gcaggaagaa ggccggggtt
     1921 ccggtgctgc tgagggtggc ggcaatcttg cggccgataa taccgctttt ccccattccg
     1981 gtaatcacga cccggccgga gcaggcggcc agtttctcca ccgccgcctc aaaattccgg
     2041 cccagcctgc gctcggtggc ctcaaccgcc tcgatctcgg agcggaagac ctcccgggcc
     2101 cgtttcagcg tttccatcgc tctttcacca cctggtcgat cgccttcgcc tcggccagca
     2161 ggccgggaag gtcgtcaagt ttaaccatgt tggggccgtc ggagcaggcg cgctccggat
     2221 ccggatgcac ctccaggaag aggccggcgc acccggcggc cagggcggcc cggaccagca
     2281 ggggcacgaa ctggcggcag ccgccgctgg tttcacccgc cgatggctgc tgcaccgagt
     2341 gggtggcgtc aaagatcacc ggcgcaaagg cgcccatcac ttccaggccg cggaaatcaa
     2401 ccaccaggtt atggtaaccg aaacaactgc cccgctcggt cagcaacacc cggcggttgc
     2461 cggtactctc gatcttgccg accaggtggc gcgtgtcttc cggcgccagg aactgcccct
     2521 tcttgacgtt caccggccgg ccggtccgcc cggccgccag cagaaggtcg gtctggcgac
     2581 agaggaaagc cggtatctgg agcacgtcca gcacttcggc ggccggggcc gcgtccgacg
     2641 gctggtgtat atccgacagg accggcaggg agagctcttc cttaacccgc cgcagaatgc
     2701 gcaggccggc ttccaggccc ggcccgcgga aagattttat ggacgtccgg ttggccttgt
     2761 cgtaggaagc tttgaaaacc aggggaaccc gtacagaaaa acaaatctcc ttgagccgcc
     2821 gggccgtttc caggatcagg gcctcggact cgatcacgca gggcccggca atcaagcaga
     2881 acggattggc atcccccagc ccgatttccc cgatttttac gatcatggaa gccgccgaag
     2941 tgtctggtac gtcctgccga cacccccggt taaccgggcg gccaacggaa ggtgcgaccg
     3001 cccagcaggt ggtagtgcag gtgaaaaacc gactggccgg ccgattcccc ggagttaaga
     3061 accagccggt aatcctggcc cgggccgacc tgctcctcgg ccacccggcg aaccgccagc
     3121 tgcaaccgac cgagcaattc ccggtccgct tcgccgaagt cggccagcga agacaggtgc
     3181 cgtttgggaa ttaccaggat gtggatcggt gcctgggggt tgatgtcccg gaaggccaga
     3241 atctcttcgt cctcgaaaac cttctgggcc ggtatttcac ccttaaccag gcggcagaac
     3301 aggcattcgg aactcataat gaacgagtga tctcctcgat ccggtaaatc acgagctgat
     3361 cgccgggcaa cgcctcgtcg gcgccatcaa gggtaaggcc gcattcgtag ccggcggtca
     3421 cttcgcgcac attctcctta aaacgcctca gcgaagcgac aataacctcg gcgaccgcct
     3481 tgtctccccg catcaggcgg gctttgccgc cccggtttac cttgccttcc agaacgctgc
     3541 agccaattgc cagctggccg ctggaaagct tgaaaatctg cttgatctga acccggccgg
     3601 tgaccacttc cttcaactcc ggctccagaa gtccggagag cgacttccga acatcatcca
     3661 gcaggtcgta aatgatccgg tattggcgga cctgaacgtt ctcctggcgg accgtctcgc
     3721 gcgccttggg atcgatattg acgttaaagg ccagcacgat gctgccggag gtcttggcca
     3781 gcaggacgtc ggcctcgccg accgggccga tgcccgagtg cagtatcctg accttcagct
     3841 cttcggtatt aagccgctgg agcgactcgg cgatggcctc catcgagccg gccacgtcgg
     3901 ccttgagtat aatgttgaga tccttggccc cctcggcctg gagacgctgg tagaactcct
     3961 cgagcgtaat cggcgtcgaa accgtcccgg cctcggggcg ggccaggttc gtttcgcaga
     4021 attcacgcgc cgtcttctcc gaatcgacca ccttgaatcg ggaggccacc ggcggcagct
     4081 ggctcaaccc gattacccgg gcggccagtc ccggggtgac ctccttcatc ggcttgccgg
     4141 catcgtcgaa gatcgcccgc acccgacccc aggtatcgcc caccgtgaaa tgatcccggt
     4201 tgcgaaggat gccgttgagc acgaccaggc tgatgttggc gccagtgccc cggtccatcc
     4261 tggtttcgat caccacgccc tcggccggac aggcgtaatc ggccttcaac tccagcatct
     4321 cggactggag caggatggcc tccagcaact caccgatccc ggtgcgcttg gcggccgaag
     4381 tttcgacgaa aagcgcctgg ccgcccaggt cttcggagag cagattatgc cgggccagct
     4441 cgcccttgat cctcgggatg ttctcccggc cccggtccac cttgttcagg gccacgatga
     4501 tcggaacctg ggcggcctga cagtggctga tggcctcaac cgtctggggc atcacgccct
     4561 cttccaccga gatcaccagg atcacgatgt cggtcagctg ggccccccgg gcccgcatgt
     4621 tggtgaaggc ctcgtggccg ggcgtgtcca agaaggtaat ccggcgcccc tcgttgctga
     4681 ccgtgtaggc cccgatatgc tgggtgatct gaccgaattc ccccccggca atattgctct
     4741 tgcggatggc gtccagcaac gaagtcttgc catggtcaac atggcccatc agggtaacga
     4801 tgggcgggcg ggccttcaaa tcttttttct tcatgttcag gcggcgctgg aaagccgggc
     4861 gttctcaagc aattcttcgg cgcccctgga atccaggccc tcgatcttgg ccaggtcctc
     4921 tggagtggtt gaaagcaggt cgttgagcga aacgataccg gcctccttga ggcgccgggc
     4981 cagttcctcg gttacgcccg gaagcgtggc gatggccggc agtgtctcgg cccgggcttc
     5041 ggattcgctc cggacatcca ccgtgtaacc ggtcagctgt gaggccagct ttacgttaat
     5101 gcccttgcgg ccgatggcca ccgccagctg gtcgtccggg accagtacgc ggctgaattt
     5161 ctgcttggcc gaaatttcga ccttggcaac cttggccggg gccagcgtgg cggcaatcag
     5221 ttcggccgga tcggcatgcc agcgaataac atccacgcgc tcgccgtcaa gttccttcat
     5281 gatcgcccgg atccgagtcc cccggatacc gatgcaggtt ccgaccgggt caatccggtt
     5341 gtcattggag gtcaccgcca ccttgcaggc ctcacccgcc acccgggcca ccgacttgat
     5401 ttcaaccagg ccctcgcgga tctccggaac ctcctgcata aagagatcct tgaccagatc
     5461 cggatgggtg gccgaaagca cgatcttcgg cccctggatc tcgcggcgca cctcgataat
     5521 gatcccccgg atcgccgacc cgcgccggcg gtgccgctcg tcccgggtca gttcggagac
     5581 cggaaaaacc ccctcgatgc gtcccaggtt taccaccagg cttccctgct cgtagtgatc
     5641 aaccaggccg gtgacgatcg ttccttcctt gcccttgaat tcgtcgtaaa gctgcgattt
     5701 ctcggcctcc cggatctttt gcatgatcat ctgccgggcg ctctgagcca tgatccggcc
     5761 cagcggcatg tcgggggccg gcaaccgctt gccggtctcg tccaccagat agacttcggt
     5821 ggtctgggga tcgatatgca gctcggtaat gttatccagc ttgagtttct tccggtaaac
     5881 cgaaagcagg ccgccctcga tagcggaaag caccgtctgg cgatccagac cgcgctcccg
     5941 ttccaggtat tcgagtatgg ctaccagctc gcggttcaac tgcatctcgg gttctcctta
     6001 aaaaatcaaa atctccaact ggaccggggc acttgagccc gtctgcaaac aagcggcccg
     6061 gccggagatt ctttgaggat ttaaataata ccattaatcc gtccaaaacg caaccggatt
     6121 gggatcgaac gctccggcgt ccggacggac atcttgcacc cgggaaggac gtcctccgcg
     6181 ccgaaaaatc tctccggcgg ggggccggcg taccgacgcg cttagggaag gaagacgagg
     6241 agggccagcg gacagccgtc gccctcgcgg ggaggcagct tcaggagccg ggtgtatcca
     6301 cccgggcgct cggcgtagcg gggcgcgatt tcgttaacca gtttccgggt caactcctgg
     6361 tctttgagaa cggagatgat ccggcgctgt ccggccagat ccttggcctt ggcgagcgtg
     6421 atcagctttt cggccgtccg ctgcacctgc cggatcctcg cctcggaagc gaccacccgt
     6481 ccattatcca ccaagccctt gagcagggag gtgaaaacac cgatccgccg accgcggttg
     6541 aggttaaccc gttttcgtct ttgatgccgc atgttaggct tccttgagtt tcagcttttt
     6601 tttggccatg aacttcttca actcttcgag gctcttttct cccagccctt ccaccgcttc
     6661 cagctcggcc tcggtgcgtc cggccagggc ctggtcggtt tcgatcccgg cggccttaag
     6721 cacgttgcgg atccgggtcg cgaggcccag cttctcaagg ctgaccccgg cggccgcggg
     6781 cgcttccgcc cgggccggac cgccgccaac ctggtccagg tgctgcttca gaatatcgcg
     6841 ggcaattatc accgtctgtt tcggttcgat ggcgccggtg gtccagattt cgagtatcag
     6901 ccgttcgtaa tcgacccgct ggccgacccg ggcggtttcg gtatggtagg cgacccgggt
     6961 gaccgggctg aaggcggccg ccagctggat cgtgccgatc ttcttctcct tctcctcgct
     7021 ctcggcggta atccggtcct cggaaacata accgaaaccg cggttcaccg tcaattcgat
     7081 ctgcagcttg gtgccggccg cgagcgaagc gatatgcaga cgcgggttta aaaccttgat
     7141 ctccgggccg gggaagaggt cctcggccac cagttcggtc ttttcggcaa tattgacaag
     7201 aaaggtcttg ggcagttccg agatgttcga tttaagttcc acctgcttca ggttcagggc
     7261 gatgtcggtg acgtcctcgg tcacgccctt gatcgtcgag aattcgtggg aaaccccggt
     7321 gatcttgaag ctggtgatgg ctacacccgg aatcgccgaa agcagcaccc gccgcaggct
     7381 gttgccgagc gtcaccccga agccccgctc cagcggctca acaatgagct tgccgtagtt
     7441 ggcccgataa ctcttctcat cccattctat cttgttcgga tagttgaact gctccatggt
     7501 tggcctcgga ccggttaacg tgaatagagg ttgacgatat gcccttccga aaccggaaga
     7561 gttacctcgc tgcgggccgg ctcgcgcagg acctttatct tcaacgcttc cttgtctacc
     7621 gcgacccagg ccggcaccac ccgcgccggc tggccggatg aagcggccac cttctccttg
     7681 atctgggggc gaatctttaa tccgggacgg aactcaactt cgtcggccgt cttggctaaa
     7741 taggaagagg cgctgacgcg cttcccgttc acccggacgt tgccgccgta aatcaactgg
     7801 cgcgcctggc gccgggaatc aatgaagccc gaaacgacca ggatgttgtc caggcgacgt
     7861 ccgggtgaac gggaagcgcg tcagcgcctc ttcctattt
//
LOCUS       DAOQYN010000079         7876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9671_length_7876_cov_5.112645, whole genome shotgun sequence.
ACCESSION   DAOQYN010000079 DAOQYN010000000
VERSION     DAOQYN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7876
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9671_length_7876_cov_5.112645"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PKN80_04365"
     CDS             <1..355
                     /locus_tag="PKN80_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35280.1"
                     /translation="VFPNWTVDRDRIGQDWKLWCERGYLDFVCPMDYTADGLIFEEQV
                     GRQRAWAGRVPCYPGIGLSVWEREGGAVKLVDFIESTRRLGTGGFTIFEYDSEGAGRI
                     APFCGAGITRPLRPR"
     gene            complement(380..1702)
                     /locus_tag="PKN80_04370"
     CDS             complement(380..1702)
                     /locus_tag="PKN80_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35281.1"
                     /translation="MRKSGRLAFFLLFLVLTLGRLAVLPAAAQTADPRAAYLMGLAAS
                     NAGDLANAETYLRQAFRMSPRNSEVISALAEVLLKTDRPQPALQAYRQAIRFNPENRS
                     AYYGAGFAYLRLKQMKLAERQYEEITVRFPDEEQAWLVLADLYTASNQNRKAVDAYHR
                     ILEARPNVPLLHYNLGLIHTRLRDYPKALEATRKTLELSPDFLPAAHLAARIYQETGR
                     NVEAESIYRELLKRFPQDRSLLLSLAQIYSEQERWTAAAVHLNRLVMTGAAEPEILLL
                     QGLIFQKEGQWERALNSYRAVLEKKPDPKIRFQTAVALDKLGRDTEAVAELRKVVAEA
                     PEDHVALNYLGYTLVLRGENLNEAEALIKRAVDLDPRNGAYLDSLGWVYFKKGDLRRA
                     GYYLEKAGRLEPDPEIFGHLAELYQKLGQPQKAAAYRQKILEMPPREE"
     gene            complement(1929..2840)
                     /gene="hisG"
                     /locus_tag="PKN80_04375"
     CDS             complement(1929..2840)
                     /gene="hisG"
                     /locus_tag="PKN80_04375"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNQ35282.1"
                     /translation="MKKSETRKKETRPGRKLVIGLPKGSLEETTVRLFRKAGLNLHGK
                     PRSYYLSADDAELEVMLIRAQEIPLYVADGTLDMGFTGKDWLTERGSRVVEVAELSYA
                     KSGLGQVKLVLAVPENSPIRGVRDLEGKRIATELVRVTQDYLKKNGVRAQVDFSWGAT
                     EIKAGKLCDAIAELTESGSTLRAHNLKIIDTIMVSTTRLVANPAAWKDAWKRRKIEYV
                     SLLLQAALNAEDLVGLKLNVCNRDLKKIMSVLPALKNPTLSPLARPGWHALEVICTRD
                     EVTHLIPRLKEAGAEGIVEYPLNRVVF"
     gene            complement(2849..3697)
                     /locus_tag="PKN80_04380"
     CDS             complement(2849..3697)
                     /locus_tag="PKN80_04380"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNQ35283.1"
                     /translation="MKEELTAWQRVQLARNPNRPHTRDYIDALVTDFLELHGDRYFGD
                     DLAMVGGLGRFDGRTVALFGHQKGRETNENIAVSFGMAHPEGYQKARRLMEMAEKFGL
                     PVLSFIDTPGAHPGMEAEERNQAGAIARNLFVMSRLRAPIVALVIGEGGSGGALGIGL
                     ADRVLMLENSVYSVISPEGCAAILWKKQEAKEAAAESLRLTAPDLLGFGIIDEIIQEP
                     AGGAHLDAKTTIEKVGLGLRKHLQELLEIPLDTLLEKRYEKYRRIGAFLEGEPPAEEP
                     APTAEE"
     gene            complement(3694..3984)
                     /gene="gatC"
                     /locus_tag="PKN80_04385"
     CDS             complement(3694..3984)
                     /gene="gatC"
                     /locus_tag="PKN80_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNQ35284.1"
                     /translation="MEEKEIRYLARLARIRLTEGEVEGLKSDLTQLLEYVDQLKGLPT
                     GAVEPTSTIVPAFNRFRADRARPSFPREAVLANAPERQADFFRVARVIKAEE"
     gene            complement(4153..6054)
                     /gene="dnaK"
                     /locus_tag="PKN80_04390"
     CDS             complement(4153..6054)
                     /gene="dnaK"
                     /locus_tag="PKN80_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNQ35285.1"
                     /translation="MAKEKIIGIDLGTSNSAASVYEGGRPTIIPSAEGQTLSGKAFPS
                     YVAFTKDGQVLVGEPARRQAVANPEGTIFGAKRNMGTDYKYRFQGKEYTPQQISAFIL
                     QKIKRDAEAFLGGEVKKAVITVPAYFNDNQRQATKDAGTIAGLEVVRLINEPTAAAFA
                     YGLDKVEQELKVMVFDIGAGTLDVTIMDLHKGVGKVISTSGDTKLGGLDMDNILIDYI
                     ANEFKKETGLDVRNDRMAIQRIREAAEKAKIELSNTLATDINLPYLTADSSGPKHLTM
                     SIRRATLEELINPILERCRGPIDQALKDAGLSAREIAKIIMVGGPTRMPSVQAFLEEY
                     VGRKAERGVDPMECVAVGAAIQGAILAGDLQDKELLLLDVTPLSLGIETLGGVSTALI
                     ARNTTIPTRKSQVFTTAADGQTAVDVHVLQGERPMATDNTSLGSFQLAGIPPAPRGLP
                     QIEVTFDIDANGILSVSAKDKGTGKEQSIKISAPNKLSKDEIDNLVRKAEEFAEADRQ
                     AKELAETANQADNLVYSIEKNLKEHGDKIGEEDRKAIESKAAALREELKSASKSVVSL
                     KKAMDELEKSSYKLAEILYKQSQAGSGQPGAPGAEAGPEAPAGPQAGAGEPEPGQGPV
                     IDAEFKEDK"
     gene            complement(6181..7239)
                     /gene="thrC"
                     /locus_tag="PKN80_04395"
     CDS             complement(6181..7239)
                     /gene="thrC"
                     /locus_tag="PKN80_04395"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNQ35286.1"
                     /translation="MELSRYSGVINRYRAHLPVTESSPVVTLLEGNTPLIPSVSIGPR
                     LCLELYFKFEGGNPTGSFKDRGMTLAVSKAREEGKQSVICASTGNTSASAAAYAARAG
                     LQALVVIPSGKIALGKLAQALIYGARVVQIEGNFDQALQLVRTLCDRYPIALVNSVNP
                     YRIEGQKTGAFEIVDSLGDAPDYHVLPVGNAGNITAYWKGYREYRRLGKAARLPRMLG
                     FQAAGAAPLVQGRPVEHPETIATAIRIGNPASWSGAVAARDESGGRIEAVSDAEITEA
                     SKTLAAGEGIFAEPASAAPVAGLLKLAAAGFFPAGSRVVAVLTGHGLKDPEFAVRECR
                     LPPPVAADPEAVARACGL"
     gene            complement(7277..>7876)
                     /locus_tag="PKN80_04400"
     CDS             complement(7277..>7876)
                     /locus_tag="PKN80_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNQ35287.1"
                     /translation="AGLALEDIRYAAELGYRVKLLSHLKYDDGHLRLATCPTLIPARH
                     LLAAVRDVYNAVFLTGDLMGESLLLGKGAGMDPTATSVVGDIVSLGREILHGVPAAPL
                     PEGAATLETRGLESRYYFRFTALDQPGVMARIAELLGRHEISISTCLQKEENPAEAVP
                     VIILTHRTRETQVRKALSEIDRLPVIKARTVMMRLAFDN"
BASE COUNT         1087 a         2470 c         2823 g         1496 t
ORIGIN      
        1 ggtcttcccg aactggacgg tggaccgcga ccggatcggc caggactgga aattgtggtg
       61 cgaacgcggc tacctggatt tcgtctgccc gatggactac actgcggacg ggctgatttt
      121 cgaggaacag gtcggccggc agcgggcctg ggcgggccgg gtgccctgtt acccgggcat
      181 cggcctcagc gtctgggagc gggagggcgg cgcggtaaaa ctggttgact ttattgagag
      241 cacgcggcgc ctgggcaccg gcggcttcac gatttttgag tacgattccg agggggccgg
      301 ccggatcgcg cccttctgcg gcgccggcat cacccggccg ctccggcccc gttaagtccg
      361 ggcgtccgcc cgcccctgac tattcctcgc gcggcggcat ctccaggatc ttctgccggt
      421 aggcggccgc cttctgcggc tggccgagct tctggtagag ttcggccagg tggccgaaga
      481 tctccgggtc gggctcgagc cggccggcct tctccaggta gtacccggcc cggcgcaggt
      541 cgcccttctt gaaataaacc cagcccagac tgtcgaggta ggcgccgttc cggggatcca
      601 ggtcgaccgc ccgcttgatc agcgcctccg cctcgttcag gttctcgccg cgcagcacca
      661 gagtgtagcc gaggtaattg agggccacgt gatcctcggg cgcctcggcc accaccttcc
      721 gcagctcggc aaccgcctcg gtgtcccgcc caagcttgtc cagcgctacc gccgtctgga
      781 agcggatttt cgggtcgggc ttcttttcga ggacggcccg gtaactgttg agggcccgct
      841 cccactgccc ctctttctga aagatcaggc cctgcagcag caggatttcc ggttcggcgg
      901 ccccggtcat gacgagccgg ttcaggtgga cggcggccgc ggtccagcgt tcctgctcgc
      961 tataaatctg ggccaggctg aggagcaggg agcggtcctg gggaaatcgt tttaggagtt
     1021 cccggtagat tgattcggcc tcgacgttgc ggccggtctc ctggtagatc cgggccgcca
     1081 ggtgggcggc cggcaggaaa tccggggaca actccagggt tttgcgggtc gcctcgagcg
     1141 ccttcgggta atcgcgcagc cgggtatgga tcaggcccag gttatagtgg agcaggggaa
     1201 cgttggggcg ggcttccagg atgcggtggt aggcgtcgac ggccttccgg ttctggttgc
     1261 tggcggtgta gaggtcggcc aggaccagcc aggcctgctc ctcatccggg aaacggaccg
     1321 tgatctcctc gtactggcgt tcggccagct tcatctgctt gaggcggagg taggcgaagc
     1381 cggccccgta gtaagccgaa cggttttcgg gattgaagcg gatcgcctgg cggtaggcct
     1441 ggagggccgg ttggggccgg tcggtcttca ggagcacctc ggccagggcc gagatgactt
     1501 cgctgttgcg gggcgacatc cggaacgcct ggcgcaggta ggtctcggcg ttggcgaggt
     1561 cgccggcatt gctggcggcc aggcccatca ggtaggcggc tcgcgggtcg gcggtttggg
     1621 cggcggccgg gagaacggcc agccgtccga gcgtcagcac caggaagagg aggaagaatg
     1681 ccaaccggcc tgatttgcgc acgggacgcc tccgactcgt ttgggggaga gtaaaaaggg
     1741 gggatggacc gggggcggaa aatcccgccc ccggtcgcga cagctcgcct cagcggcgct
     1801 cagcgttccc gctttttatg gcggtccctt cgaagccgct tctttctctt gtgcttagcg
     1861 atcttctttc tttttcgttt tcttccgcag ggcattctgc ctccgtgaag tgagcggtgg
     1921 tttccgtttt agaataccac ccggttgagc ggatactcca caatcccttc ggcgccggcc
     1981 tccttcaacc gggggatcag atgggttacc tcgtcgcgcg tgcagatgac ttccagcgcg
     2041 tgccagccgg gccgcgccag gggcgagagg gtggggttct tcagggccgg cagcaccgac
     2101 atgattttct tcaggtcccg gttgcagacg ttaagcttga ggccgaccag gtcctcggcg
     2161 ttgagcgccg cctggagcag caggctgaca tactcgatct tccggcgctt ccaggcgtcc
     2221 ttccaggcgg cggggttggc caccagccgg gtggtcgaga ccatgatggt gtcgatgatc
     2281 ttgaggttgt gggcgcggag cgtggaaccg gattcggtca gttcggcgat ggcgtcgcag
     2341 agtttgccgg ccttgatctc ggtggccccc caggagaaat cgacctgggc ccggacgccg
     2401 ttcttcttca ggtaatcctg ggtgacccgg accagctcgg tggcgatccg cttgccttcc
     2461 aggtcgcgca cgccgcggat cggggaattc tccggaaccg ccaggaccag tttcacctgg
     2521 cccaggccgc tcttggcgta ggagagttcg gccacctcga ccacccggga gccgcgctcg
     2581 gtcagccagt ccttgccggt gaagcccatg tcgagcgtgc cgtcggccac gtaaaggggg
     2641 atctcctggg cccggatgag catgacttcc agctcggcgt cgtcggcgct caggtagtag
     2701 gaacggggct tgccgtgcag gttgaggccg gccttgcgga agaggcggac ggtcgtctcc
     2761 tcgaggctgc ccttgggcag gccgatgacc agcttgcggc cgggccgggt ctctttcttc
     2821 ctggtttcag acttcttcat aagtgttttt actcttctgc agttggcgcc ggttcctccg
     2881 ccggcggctc gccctcgagg aaggcgccga tgcgccggta cttctcatag cgcttttcca
     2941 gcagggtgtc cagcgggatc tccagcagtt cctggagatg cttcctgagt cccaggccga
     3001 ccttctcgat ggtggtcttg gcgtccaggt gcgcgccgcc ggccggttcc tggattatct
     3061 cgtcaatgat gccgaagccg agcaggtcgg gcgcggtcag gcgcagggat tcggcggcgg
     3121 cctccttggc ctcctgcttc ttccagagga tggccgcgca gccctcgggc gagatgaccg
     3181 agtaaacgct gttttcaagc atcagcaccc ggtcggccag gccgatgccg agcgcgccgc
     3241 cgctgccgcc ctcgccgatg accagggcga cgatcggggc ccggagacgg gacatcacga
     3301 aaaggttgcg ggcgatggcc ccggcctggt tgcgttcctc ggcctccatg cccggatggg
     3361 cccccggcgt atcgatgaaa ctgaggaccg gcaggccgaa tttctcggcc atctccatca
     3421 ggcgccgcgc cttctggtag ccctcggggt gggccatgcc gaagctgacc gcgatatttt
     3481 cgttggtctc gcgccccttc tggtggccga agagcgccac cgtccggccg tcaaagcggc
     3541 ccagcccgcc gaccatggcc aagtcgtccc cgaagtagcg gtcgccgtgg agttccagaa
     3601 aatcggttac cagggcgtct atgtaatctc gggtgtgcgg tcggttcggg ttgcgggcca
     3661 gctggacccg ctgccaggcg gtcagttctt ccttcattcc tcggccttga tgacccgggc
     3721 gacccggaag aagtccgcct gccgttccgg cgcgttggcc aggaccgctt ccctgggaaa
     3781 cgaggggcgc gcccggtcgg cgcggaaccg gttgaaggcg ggcacgatgg tgctggtcgg
     3841 ctcgacggcc ccggtgggca ggcccttgag ctggtcaaca tactccagca gctgggtcag
     3901 gtcggacttg agcccctcga cctcgccctc ggtcaaccgg atgcgggcca gccgcgccag
     3961 gtaacggatc tctttttctt ccatcggcaa aaccgccttt aaagggtttt acggcaacgt
     4021 tagattataa gacgggcaaa gcgtttccgt caagaaaacc ggccgcgctt ccggcgccgg
     4081 gagacgaaaa aaggggcgcc ccgaaagaat cggggcgccc ccggtagttc tccgctcttt
     4141 caagggccgg ccttacttgt cctccttgaa ctcggcgtcg atcaccggac cctggcccgg
     4201 ctccggttcg ccggcgccgg cctgggggcc ggccggcgct tccgggccgg cttcggcgcc
     4261 cggcgcgccc ggctggccgg acccggcctg ggactgcttg tagaggatct cggccagctt
     4321 gtaactggac ttttccagtt catccatcgc cttcttgagc gagacgacgc tcttggaggc
     4381 ggatttcagt tcctcgcgca gggcggccgc cttgctctcg atcgccttgc ggtcctcctc
     4441 gccgatcttg tcgccgtgct ccttgaggtt cttctcgatg ctgtaaacca ggttgtcggc
     4501 ctggttggcc gtttcggcca gttccttcgc ctggcggtcg gcctcggcga actcctcggc
     4561 cttccggacc aggttgtcga tctcgtcctt ggagagcttg ttgggggccg agatcttgat
     4621 ggactgctcc ttgccggtgc ccttgtcctt ggccgagacg ctgagaatgc cgttggcgtc
     4681 gatgtcgaag gtcacctcga tctggggcag gccgcgcggg gccggcggga tgccggccag
     4741 ctggaagctg ccgagcgagg tgttgtcggt ggccatcggc cgctcgccct ggagcacgtg
     4801 aacgtcgacc gcggtctggc cgtcggcggc ggtggtgaag acctggctct tgcgggtcgg
     4861 gatggtggtg ttgcgggcga tgagcgccgt ggagacgccg cccagggtct cgatgcccag
     4921 cgagagcggc gtgacgtcca gcagcaggag ttccttgtcc tggaggtcgc cggccaggat
     4981 ggctccctgg atggccgcgc cgacggccac gcattccatc gggtcgacgc cgcgctcggc
     5041 cttgcggccg acgtactcct cgaggaaggc ctggaccgag ggcatccggg tcgggccgcc
     5101 gaccatgatg atcttggcga tctccctggc ggagaggccg gcgtccttga gagcctggtc
     5161 gatcggaccc cggcagcgct ccaggatggg gttgatcagc tcctcgaggg tggcgcgccg
     5221 gatggacatg gtcaggtgct tggggccgct gctgtcggcg gtcaggtacg gcaggttgat
     5281 gtcggtcgcc agggtgttgg agagttcgat cttggccttc tcggccgcct cccggatgcg
     5341 ctggatggcc atccggtcgt tgcgcacgtc caggccggtc tccttcttga actcgttggc
     5401 gatgtagtcg atcaggatgt tgtccatgtc caggccgccc agcttggtgt cgccgctggt
     5461 ggagatgacc ttgccgacgc ccttgtggag gtccatgatg gtgacgtcga gggtgccggc
     5521 gccgatgtcg aagaccatca ccttcagttc ctgctcgacc ttgtccaggc cgtaggcgaa
     5581 ggcggcggcg gtcggctcat tgatcaggcg gaccacctcg aggccggcga tggtgccggc
     5641 gtccttggtg gcctggcgct ggttgtcgtt gaagtaggcc ggcaccgtga tgaccgcctt
     5701 cttcacctcg ccgcccagga aggcctcggc gtcgcgcttg atcttctgga ggatgaaggc
     5761 cgagatctgc tggggcgtgt actccttgcc ctggaagcgg tacttgtagt cggtgcccat
     5821 gttgcgcttg gccccgaaga tggttccctc ggggttggcg accgcctggc gtcgggccgg
     5881 ttcgccgacc agcacctgtc cgtccttggt gaaggccacg taggagggga aggccttgcc
     5941 cgagagggtc tgcccctcgg cgctggggat gatggtcggc cggccgccct cgtagaccga
     6001 ggccgccgag ttgctggtgc ccaggtcgat gccgatgatc ttctcttttg ccatggtagt
     6061 gctccttgat ggtttcgtcc gggaagggcg ccggaatcgg cggcggcagg gccggggaag
     6121 gcggcggact tttccggggg ttttggttac agaagattag atgcgccgcc gccggcctgg
     6181 ttacagcccg caggcccggg ccaccgcctc cgggtcggcc gccaccggcg gcggcagccg
     6241 gcactcgcgc accgcgaact ccgggtcctt gaggccgtgg ccggtgagca cggccaccac
     6301 ccggctgccc gccggaaaga agccggccgc ggccagcttg aggaggccgg ccaccggggc
     6361 ggccgaggcc ggctcggcga agatgccctc gccggcggcc agggtcttgg aggcctcggt
     6421 gatttcggcg tcgctgaccg cctcgatccg gccgccggac tcgtcgcggg cggccacggc
     6481 ccccgaccag ctggccgggt tgccgatgcg gatggcggtg gcgatggtct cgggatgctc
     6541 gaccggccgg ccctggacca gcggcgcggc gccggcggcc tggaagccca gcatccgggg
     6601 cagccgggcg gccttgccga gccggcggta ctcccggtag cccttccagt aggcggtgat
     6661 gttgccggcg ttgccgaccg gcaggacgtg gtagtcgggg gcgtcgccca ggctgtcgac
     6721 gatctcgaag gcgccggtct tctgcccctc gatccggtag gggttgaccg agttgaccag
     6781 ggcgatcggg taccggtcgc agagggtgcg taccagctgc agggcctggt cgaagttgcc
     6841 ctcgatctgg accacccggg cgccgtagat gagggcctgg gccagcttgc cgagggcgat
     6901 cttgccggag gggatgacta ccagcgcctg gagtccggcc cgggcggcgt aggcggccgc
     6961 cgaggccgag gtgttgccgg tcgaggcgca gatgaccgat tgcttgccct cctccctggc
     7021 cttggagacg gccagggtca tgccccggtc cttgaaggag ccggtcgggt tgccgccttc
     7081 aaacttgaag tagagttcca ggcagaggcg ggggccgatg gagaccgagg ggatgagcgg
     7141 ggtgttgccc tccagcaggg tgaccaccgg gctgctctcg gtgaccggca ggtgggcccg
     7201 gtaacggttg atgacaccgc tgtaacggct cagttccatg tttcgatctc tccgggaaag
     7261 cgggcggcgc cggcgttcag ttgtcgaagg ccagccgcat catgaccgtc cgggccttga
     7321 tgaccggcag ccggtcgatt tcgctcagcg ccttgcgtac ctgggtttcg cgggtccggt
     7381 gggtcaggat gatgaccggc accgcctcgg ccgggttctc ctccttctgt aagcaggtgg
     7441 agatggagat ctcgtggcgg ccgagcagct cggcgatccg ggccatcacg cccggctggt
     7501 cgagggcggt gaagcggaag tagtagcggc tttcgaggcc gcgggtctca agggtcgccg
     7561 cgccctccgg caggggggcg gccggcacgc cgtgcaggat ctcccggccc aggctgacga
     7621 tgtcgcccac gacgctggtg gcggtcgggt ccatgccggc ccccttgccc agcagcaggc
     7681 tctcgcccat caggtcgccg gtcaggaaga ccgcgttgta aacgtcgcgg acggcggcca
     7741 gcaggtggcg ggccgggatg agggtcggac aggtggccag ccgcaggtgg ccgtcgtcgt
     7801 acttcaggtg cgagaggagt ttcacccggt agccgagttc ggccgcgtag cggatgtcct
     7861 cgagggccag gccggc
//
LOCUS       DAOQYN010000080         7836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9722_length_7836_cov_4.504177, whole genome shotgun sequence.
ACCESSION   DAOQYN010000080 DAOQYN010000000
VERSION     DAOQYN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9722_length_7836_cov_4.504177"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKN80_04405"
     CDS             <1..212
                     /locus_tag="PKN80_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNQ35288.1"
                     /translation="DERCANMVVAGYVAGAAAGRFDPFPGLAALEKALPAVFPGKKNV
                     IALNEEALRSGYRQAIPRPAGRRSD"
     gene            234..1340
                     /gene="galK"
                     /locus_tag="PKN80_04410"
     CDS             234..1340
                     /gene="galK"
                     /locus_tag="PKN80_04410"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNQ35289.1"
                     /translation="MVTAQSPGRVEILGNHTDYNEGFVLLAAIDLRTTVSGRRASGSR
                     GRIFSARLDQSCEFSLDRVEKDPERAWADYPKGVVRELRRLGVSLPGFEARIESALPF
                     GAGLSSSAALELAFGFFLQGLAGFDLSRLEMVKLCRRAENEFVGVGCGILDQFGCAFG
                     RADSLLWLDCRTLDYEVFRLPARAAVVVCDSGRGRRLAASGYNRRRRECSAALDFFAG
                     RRPGLAALRDLEPEDFRRLAPGLADPALRNRAEHVVNENRRVREAAGVLRRGDLARLG
                     ELMRASHSSSRDLFENSTPELDFLVETAYRQPGIIGAKLSGAGWGGATVNLVAAGAAG
                     DFARRLRAAFRAEFGREPAVYLASIPDGAGLVND"
     gene            1482..2423
                     /locus_tag="PKN80_04415"
     CDS             1482..2423
                     /locus_tag="PKN80_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020617527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ35290.1"
                     /translation="MAEYLVTGGAGFIGSHLVERLVREGGSVRVLDNFSSGRESNLAG
                     VAGPGVEVLRGDICDQAACAEACRGVSYVVHLAALRSVPRSMADPYSYNRVNIDGSLN
                     MMFAARAAGVRQFALASSSSVYGEAERFPQSEAQLPALISPYALSKLAGEHYGYIFTR
                     QFGLPVTCLRYFNVFGERQSLEDEYAVVIPKFITCMLKGERPPVHGDGTQSRDFTPVE
                     NVAEATLLAVRNPAAAGLVFNVGCGREHSILNLVAELNRILGSNLEPLFTPVRPGDVM
                     RTLADIGRARRVLGYEPRVSFADGLARTVEYFKKTLA"
     gene            2462..3643
                     /locus_tag="PKN80_04420"
     CDS             2462..3643
                     /locus_tag="PKN80_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HNQ35291.1"
                     /translation="MGMWIGRGRKARRCYGFDEVALVPGRRTVDPALVDTSWDLAGRK
                     FALPIVAAAMDGVVDVRMSVEMGRTGSLAVLNLAGIQTRYENPGEVIEKIVGASQSEV
                     TALIQSVYREPVKEKLIGRRIKEIKDAGGIGAVSAIPQQAIRWGEIAVKSGVDIFVIQ
                     ATVTSVNHVAERYRPLDLGAFCKAMPAPVIVGNCVNYEVALELMEAGASGLLVGIGPG
                     AACTTREVLGIGVPQITATCDCAAARDFYFKMHGRYVPIITDGGMTGSGDICKAFAAG
                     ADSVMVGSMLARAEEAPGRGYHWGMATSDENLPRGTRVRVGVTGSLKEILLGPARVDD
                     GSQNLVGALRTSMGSLGCLNLREMQLVEICIAPAIKTEGKVFQSARKAAQFMPDAGRD
                     S"
     gene            3751..7194
                     /gene="dnaE"
                     /locus_tag="PKN80_04425"
     CDS             3751..7194
                     /gene="dnaE"
                     /locus_tag="PKN80_04425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNQ35292.1"
                     /translation="MADFVHLHVHTQYSLLDGMCRIEAVTEQARKWGMPALAVTDHGK
                     MFGAVEFYKTAIKNGVKPIIGSEFYLAPRSRFDKSGERSSDAYSHLTLLARDETGYRN
                     LLILSSLSHTEGFYHRPRIDKELLKEYHEGLLLLSGCLKGEINTALLQNNEAEAKRVA
                     DFYREIFGPDFFLEVQYNGLPEQKAVLPRMVELSRQTGIPLVATNDCHYLRREDSLAH
                     DALLCIQTGSLVDEVRRLKFSSDEFYFRSAEEMAAIFKDLPEAVKNTLAVAERCNLKL
                     EFGRARLPHWNPPEGYTLAGYLRHLVEAGARRRYGLDMSAPGAGQEAVVQRINTELSV
                     IEKMGYPGYFLIIQDIVNFARGQGIAVGPGRGSAAGSVVAYLAGITDIDPLQYNLLFE
                     RFLNPDRVSLPDIDIDFEDIRREEVIAYIRSKYGANQVAQIVTFGTLSARSVIRDVGR
                     VLGMPYAEVDRIAKLVPAELHITLDEALSRERELADLASGDETTRRLFDISRSLEGLP
                     RHASTHAAGVVIGAGPLYEDAPLFLGTDDAVTTQYDMNILGEVGLLKVDLLGLKTLTV
                     LKQAGELVKQRYGKDLDNLPLDDPATYRMLSAGNSFGIFQLESSGMRDLLRKISPGVF
                     DDLIAILALYRPGPLGSGMVENFISGKKDDSRITYDHKMLVDILKPTYGVILYQEQVM
                     EIVHRMGKFTLAESDLFRRAMGKKTPEIMEENRTRFLKGARANGVSERTAEKIFNQIV
                     KFAGYGFNKSHSAGYALITYQTAYLKANHPLEFMASLLTSEIGNSEKIVEYIGECERM
                     KIWVLPPCVEESQANFTVFGNDIRFGLAAIKNVGYPAIESIVKARQAGGPFKDLFDFC
                     RRIDLRLVNRKVLESLVKAGALDLFNQPRNRLMAMISPALDQAGSYQEEARLGQGNIF
                     GESAGPANIQLPREIAELPEWSELELLAGEKEVLGLYLSGHPLGHYEQVIGAFATHTV
                     RQLRSLSPGTQVRLGGIIERVKKLVTKKEGKRMASFSFEDLTGKLEALAFPDTFEKSG
                     GYLHNGHIVFLEGKLEIREDRPQLVVSEVVPVSEGLVHYAQKVEVAFFPAGLEDDVLE
                     KFRNLAARYPGKAALVLRLRFTGRRAVRLKLDNGYRVAPEESFIQAVEALFGPDSITV
                     A"
     gene            7304..7480
                     /locus_tag="PKN80_04430"
     CDS             7304..7480
                     /locus_tag="PKN80_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35293.1"
                     /translation="MDKVFLLSGLLALVCLLLSALSGWLRLKLKWHRLLALLALVFAL
                     VHAGLWAYFNFFSG"
     gene            7505..>7836
                     /locus_tag="PKN80_04435"
     CDS             7505..>7836
                     /locus_tag="PKN80_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35294.1"
                     /translation="MKRAIWISLAAAVLVGLSAGPLRAHPPREVKLAFEAGRRQLTVE
                     VSHQVPNEKRHYINRIEVTLDGRETVTQLCRRQTDQRTQTVVYLLPDAEPGSKIKVSV
                     TCNLGGTR"
BASE COUNT         1297 a         2656 c         2547 g         1336 t
ORIGIN      
        1 gcgacgaacg ctgcgccaac atggtggtgg ccggttacgt cgccggcgcc gccgccggcc
       61 ggttcgatcc ctttccgggg ctggccgccc tcgaaaaggc cctgccggcg gtcttccccg
      121 gcaagaagaa cgtcatcgcc ctcaacgaag aggccctgcg gtccggttac cggcaggcga
      181 tcccccggcc ggccggccgc cgttccgact gaaccgccgg cccgccccaa accatggtga
      241 ccgcccagtc gcccggccgg gtcgagattc tcggcaatca caccgattac aacgagggct
      301 ttgtcctgct ggccgccatc gacctcagga ccaccgtttc cggccggcgc gcgtccggtt
      361 cccgcggccg gatcttctcg gcccgcctcg accagtcctg cgaattctcc ctcgaccggg
      421 ttgaaaagga tcccgaacgc gcctgggccg attaccccaa gggagtcgtc cgggaattgc
      481 ggcggctcgg ggtttccctg cccggcttcg aggcgcggat cgaaagcgcg cttcctttcg
      541 gggccggcct gagcagctcg gccgccctgg aactggcctt cggcttcttt ctgcaggggc
      601 tggccggttt cgacctctcc cggctggaga tggtcaagct ctgccggcgg gccgagaacg
      661 agttcgtcgg ggtcggctgc ggcatcctcg accagttcgg ctgcgccttc ggccgggccg
      721 attccctgct ctggctggac tgccgcaccc tcgattacga ggtcttccgc ctgccggccc
      781 gggcggcggt ggtcgtctgc gattccggcc gcggccgccg gctggccgcc tccggctaca
      841 accggcgccg ccgggaatgc tcggccgccc tcgatttctt cgccggtcgc cggcccggcc
      901 tggccgccct gcgcgacctg gaaccggagg atttccggcg gctggctccc ggcctggccg
      961 acccggcgct ccggaaccgg gccgaacacg tggtcaacga gaaccggcgg gtccgggagg
     1021 cggccggggt gctgcgccgc ggggacctgg cccgcctggg cgaactgatg cgggcctccc
     1081 attccagttc gcgcgacctc ttcgaaaact ccacgccgga acttgacttc ctggtggaga
     1141 ccgcctaccg gcagccgggg atcatcggcg ccaagctctc cggggccggc tggggcggcg
     1201 ccaccgtcaa cctggtggcc gccggcgccg ccggggattt cgcccgcaga ttacgggccg
     1261 ccttccgggc cgaattcggc cgcgaaccgg ccgtttacct ggcctccatc ccggatggcg
     1321 ccggtcttgt taacgattga ccggggcggt tttgaagaat tccgccgccg gtttgcgtcg
     1381 gggttggccc ttgtagtaaa attaatttat ggtagcctta ctccccccta aaacaaacct
     1441 ttccggccgt ccttccggct gcctttcccg ggttggcaag catggctgaa tacctggtga
     1501 ccggcggcgc cggcttcatc ggcagccacc tggtggaacg cctggtccgg gaaggcggtt
     1561 cggtgcgcgt cctggacaac ttcagctccg gccgcgagtc caacctggcc ggggtcgccg
     1621 gcccgggagt ggaggtgctc cggggcgaca tctgcgacca ggcggcctgc gccgaggcct
     1681 gccggggcgt ctcttacgtc gttcacctgg ccgccctgcg ctcggtgccc cgttcgatgg
     1741 ccgatcccta cagctacaac cgggtcaaca tcgacggcag tctgaacatg atgttcgcgg
     1801 cccgggcggc cggggtcagg cagttcgccc tggcctcctc cagctccgtt tacggcgagg
     1861 ccgaacgctt cccccagtcc gaggcgcagc tgccggccct gatctctccg tacgccctct
     1921 ccaagctggc cggcgagcat tacggctaca tcttcacccg ccagtttgga ctgccggtca
     1981 cctgtctccg ctacttcaac gtcttcggtg aacggcagag cctcgaggac gagtacgcgg
     2041 tggtcatccc caagttcatc acctgcatgc tcaagggtga acggccgccg gtccacggcg
     2101 acggcaccca gagccgcgat ttcaccccgg tcgagaacgt ggccgaggcc accctgctgg
     2161 cggtgcgcaa cccggccgcg gccgggctgg tcttcaacgt cggctgcggc cgggagcact
     2221 ccatcctgaa cctggtggcc gagctcaacc gcatcctggg ttcgaacctg gaaccgctct
     2281 tcacgccggt tcggcccggc gacgtcatgc ggacgctggc cgacatcggc cgggcgcgcc
     2341 gggtcctggg ctacgagccg cgggtctcct ttgccgacgg cctggcccgg acggtcgaat
     2401 acttcaagaa aacgctcgct taaactaaaa accataatct tcgccttcaa gggagtcgac
     2461 catgggaatg tggattggca gaggcaggaa agcccgccgt tgttacggct ttgacgaagt
     2521 ggccctggtg cccggccgcc gcacggtgga cccggccctg gtcgacacct cctgggatct
     2581 ggccggccgg aagttcgccc tgcccatcgt ggccgcggcc atggacggag tggtggatgt
     2641 ccggatgtcg gtcgagatgg gccggaccgg cagcctggcg gtactcaacc tggccggcat
     2701 ccagacccgg tacgaaaatc ccggcgaggt gatcgagaag atcgtcggcg cctcccagtc
     2761 ggaggtgacc gccctgatcc agtcggtcta ccgggaaccg gtcaaggaga agctcatcgg
     2821 tcgccggatc aaggagatca aggatgccgg cggcatcggc gccgtctcgg ccattcccca
     2881 gcaggcgatc cgctggggcg agatcgcggt gaagtcgggt gtcgacatct tcgtcatcca
     2941 ggccaccgtg accagcgtca accatgtcgc ggagcgttac cggcccctgg acctgggcgc
     3001 cttctgcaag gcgatgccgg cgccggtcat cgtgggcaac tgcgtgaatt acgaggtggc
     3061 cctggaattg atggaggcgg gcgccagcgg actcctggtg ggcatcggcc cgggcgccgc
     3121 ctgcaccacc cgcgaggtgc tgggcatcgg ggtcccccag atcaccgcca cctgcgactg
     3181 cgccgccgcg cgcgattttt acttcaagat gcacggtcgt tacgtcccga tcatcaccga
     3241 cggcggcatg accggcagcg gggacatctg caaggccttc gccgccggcg ccgattcggt
     3301 gatggttggt tcgatgctcg cccgggccga agaggcgccg ggccgcggct accactgggg
     3361 catggccacc tcggacgaga acctgccgcg cggcacccgg gtgcgggtgg gcgtcaccgg
     3421 cagcctgaag gagatcctgc tggggccggc ccgcgtcgat gacggcagcc agaacctggt
     3481 cggcgccctg cgtacctcga tgggcagcct gggctgtctc aacctgcgcg agatgcagct
     3541 ggtcgagatc tgcatcgcgc cggccatcaa gaccgagggc aaggtcttcc agagcgcccg
     3601 caaggccgcc cagttcatgc ccgacgccgg ccgcgattcc tgaccggccg ccggcgccgc
     3661 gccggtttcc cgccgcccct ccgcggcggc gggtcgttcc ttggtaaatt cagccgttcg
     3721 tctcctctcc tgaaaccggg ctcctgaccc atggccgatt tcgtacacct gcacgttcac
     3781 acccagtaca gcctcctgga cggcatgtgc cgcatcgagg cggtgaccga gcaggcccgg
     3841 aagtggggta tgcccgccct ggcggtcacc gaccacggca agatgttcgg ggcggtcgag
     3901 ttctacaaga cggccatcaa gaacggcgtc aaaccgatca tcggcagcga gttctacctg
     3961 gcgccccgca gccggttcga caagtcgggt gaacgcagca gcgacgctta ctcccacctg
     4021 accctgctgg ccagagacga gaccggctac cgcaacctgc tcatcctctc cagcctttcc
     4081 cacaccgagg gattctacca ccggccccgg atcgacaagg aactgctcaa ggagtaccac
     4141 gagggcctgc tgctcctcag cggctgcctc aagggcgaga tcaacaccgc cctgctgcag
     4201 aacaacgagg ccgaggcgaa gcgggtggcc gatttctacc gggagatctt cggtcccgat
     4261 ttcttcctgg aggtgcagta caacggcctg ccggagcaga aagcggtcct gccccggatg
     4321 gttgaattga gccgccagac cggtatcccg ctggtggcca ccaacgactg ccattacctg
     4381 cggcgggaag acagcctggc ccacgacgcc ctgctctgca tccagaccgg cagcctggtg
     4441 gacgaagtcc ggcgcctgaa gttctcctcc gacgagtttt atttccggag cgccgaggag
     4501 atggcggcca tcttcaagga tctgcccgag gccgtcaaaa acaccctggc ggtggccgaa
     4561 cgctgcaacc tgaaactgga attcggccgg gccaggctgc cccactggaa cccgcccgaa
     4621 ggctataccc tggccgggta cctgcgccac ctggtcgagg cgggtgcccg ccgccgctac
     4681 ggcctggaca tgtcggcgcc cggcgccggc caggaagcgg tggtgcagcg catcaacacc
     4741 gagctctcgg tcatcgagaa aatggggtat cccggctact tcctcatcat ccaggatatc
     4801 gtcaacttcg cccgcgggca gggcatcgcg gtcggtccgg gccggggctc ggccgccggc
     4861 agcgtggtgg cctacctggc cgggattacc gatatcgatc ccctccagta caacctgctc
     4921 ttcgaacgct tcctgaaccc ggaccgggtc agcctcccgg atatcgacat cgatttcgag
     4981 gacatccgcc gggaagaggt gatcgcctac atccggagca agtacggcgc caaccaggtg
     5041 gcccagatcg tcaccttcgg gaccctttcg gcccgttcgg tgatccgcga cgtgggacgg
     5101 gtgctgggca tgccgtacgc cgaggttgac cggatcgcca agctggtgcc ggccgaactt
     5161 cacatcaccc tggacgaagc gctcagccgg gaacgggaac tggccgacct ggcctccggc
     5221 gacgaaacga cgcgccgcct cttcgatatt tcccggagcc tggagggatt gccgcgccac
     5281 gcctccaccc acgccgccgg ggtcgtcatc ggcgccggtc cactctacga ggacgcgccg
     5341 cttttcctgg ggaccgatga cgcggtgacc acccagtacg acatgaacat cctgggcgag
     5401 gtcggcctgc tcaaggttga cctgctcggc ctgaagaccc tgaccgtgct gaagcaggcc
     5461 ggggaactgg tcaagcagcg ttacggcaag gacctcgata acctgccgct cgacgacccg
     5521 gccacctacc ggatgctttc ggccgggaat agtttcggca ttttccagct ggagagcagc
     5581 gggatgcgcg acctgctgcg caagatcagc ccgggcgtct tcgacgacct gatcgccatc
     5641 ctggcccttt accggccggg tccgctgggc agcggcatgg tcgagaactt catcagcggc
     5701 aagaaggacg actccaggat cacttacgac cacaagatgc tggtggatat cctgaagccg
     5761 acctacgggg tcattctcta ccaggaacag gtgatggaga tcgtccaccg gatggggaaa
     5821 ttcaccctgg ccgagagcga tctcttccgg cgggcgatgg gcaagaagac ccccgagatc
     5881 atggaggaga accggacccg ttttctgaag ggggcccggg ccaacggggt ctccgagcgg
     5941 acggccgaaa agatcttcaa ccagatcgtc aagttcgccg gttacggttt caacaagagc
     6001 cacagcgccg gttacgcgct catcacctac cagaccgcct atctcaaggc caaccatccg
     6061 ctcgaattca tggcctccct gctcaccagc gaaatcggca acagcgagaa gatcgtcgag
     6121 tacatagggg agtgcgagcg gatgaagatc tgggtgctgc ctccctgcgt cgaggaaagc
     6181 caggccaact tcaccgtttt cggcaacgac atccggttcg gcctggcggc catcaagaac
     6241 gtcggctacc cggccatcga gtcgatcgtc aaggcccggc aggcgggcgg ccccttcaag
     6301 gatctctttg atttctgccg ccggatcgac ctccggctgg tcaaccggaa ggtgctggag
     6361 agcctggtca aggccggtgc cctggatctt ttcaaccagc cgcgcaaccg gctgatggcc
     6421 atgatttccc cggccctgga ccaggccggc tcctaccagg aggaggcgcg cctcggccag
     6481 ggcaacatct tcggggagag cgccggcccg gccaacatcc agctgcccag ggagattgcc
     6541 gaattgccgg aatggtccga actggaactg ctggccggcg aaaaggaggt gctcggcctc
     6601 tacctctccg gccacccgct tggacattac gaacaggtga tcggtgcctt cgccacccat
     6661 accgtccgcc agctgcgcag cctctcgccc gggacccagg tgcggctggg cggcatcatc
     6721 gaacgggtga agaaactggt gaccaagaag gagggcaagc gcatggcctc cttcagcttc
     6781 gaggacctta ccggcaagct cgaggcgctc gcctttcccg ataccttcga aaagagcggc
     6841 ggctacctgc acaacggcca cattgtcttc ctggagggga agctggagat ccgggaggac
     6901 cggccccagc tggtggtcag cgaggtggtg ccggtctccg agggactggt ccactacgcc
     6961 cagaaggtcg aagtggcctt cttcccggcc gggctggagg acgacgtgct ggaaaagttt
     7021 cgcaacctgg cggctcgata ccccggcaag gcggcgctgg tgctgcgcct gcggtttacc
     7081 ggccgccggg ccgtgcgtct caagctggac aacggctacc gggtggcgcc ggaggaatcg
     7141 ttcatccagg cggtcgaagc gctcttcggt ccggattcca tcacggtcgc ctgagcggct
     7201 ggtcccggcc cggtttttac aaagcggcgg ccggccggta taataaaccg gttttccggt
     7261 tctctggatt ctgccggcgg cgcggccggg agaggagctg aaaatggaca aggttttcct
     7321 gctttccggc ctgctggccc tggtctgcct gctgctttcg gccctgagcg gctggttgag
     7381 gctcaagctg aagtggcacc ggctgctggc cctgctggcc ctggtgtttg ccctggttca
     7441 cgccggtctg tgggcctact tcaatttctt ttccggttag attttttgcc aacgggaggt
     7501 ttcgatgaaa agggcgatct ggatatcgct ggccgcggcg gtcctggtcg gactgtcggc
     7561 cggtccgctc cgggcgcacc cgccccggga ggtgaagctg gccttcgaag cgggccgccg
     7621 gcagctgacc gtggaggtca gccaccaggt gccgaacgag aagcggcact acatcaaccg
     7681 gatcgaggtg acgctggacg ggcgcgaaac agtcacccag ctttgccggc gccagaccga
     7741 ccagcggacc cagacggtgg tttacctgct tccggacgcc gagcccggct ccaaaatcaa
     7801 ggtcagcgtc acctgtaacc tcggaggtac ccggag
//
LOCUS       DAOQYN010000081         7711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9882_length_7711_cov_3.959378, whole genome shotgun sequence.
ACCESSION   DAOQYN010000081 DAOQYN010000000
VERSION     DAOQYN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9882_length_7711_cov_3.959378"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..1307
                     /locus_tag="PKN80_04440"
     CDS             273..1307
                     /locus_tag="PKN80_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNQ35295.1"
                     /translation="MGKFFNFRFSTDIGIDLGTANSVVYVRGKGIVLSEPSIVAIDRE
                     THKMIAVGKEAKKMLGRTPTNIIAMRPMRDGVIADFEATEAMLRHFLDQVRSTRHRFF
                     LPPRVVIAIPSGITSVEKRAVIETATKAGARSVYLIEEPKAAAIGIGLPVGEPIGNFI
                     IDIGGGTTEMAVISLGDIVLPRSIRIGGNEMDESITEYLKKKYNLLIGERTAEEIKIQ
                     IGSAWPLEEEMSLDVTGRDLVEGLPRRITVRSEEIRESLQENLTAIINEVRALLENTP
                     PELSSDLAVRGIYMAGGGSLLRGIDRLLSSETKIPVQVADEPLLAVVRGTGRMLEEEK
                     YFTKIQPYQT"
     gene            1394..2062
                     /gene="mreC"
                     /locus_tag="PKN80_04445"
     CDS             1394..2062
                     /gene="mreC"
                     /locus_tag="PKN80_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNQ35296.1"
                     /translation="MLWRFQKEILLVVFLLISFTLTNLKISGGRTGDRTFLKLEEYHL
                     QFEQEVLRENQRLRRLLGMKERRANRILAIGGVVGIDPMGSPSWLLAEVGNPEALRPD
                     LAALDGRGNLVGRVVEIKGRTLRVMTILNPQSRVSVVLQESRNLGVLESGEAGSLKIN
                     YIPREATVLEKEAVMTSQLSHNFPAGIPIGRVQRIAKKGFFTEVTVLPNANFSTLEEV
                     AFAH"
     gene            2052..2537
                     /gene="mreD"
                     /locus_tag="PKN80_04450"
     CDS             2052..2537
                     /gene="mreD"
                     /locus_tag="PKN80_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNQ35297.1"
                     /translation="MPINPRNFIFVILANFGVQYLAGRYLIIFDTIPDLNLLLVLYLS
                     FHFVDATGLMAAFALGIFHDLVFRYQLGSHSLVYVAIAYLANRAVPGRFELTPAKSFI
                     LCFYFAFLFAIGYEMLLPPAWGAGIFWKHSLFAFYSSAWSLPAFFLYELGFQQTREPV
                     F"
     gene            2539..4272
                     /gene="mrdA"
                     /locus_tag="PKN80_04455"
     CDS             2539..4272
                     /gene="mrdA"
                     /locus_tag="PKN80_04455"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNQ35298.1"
                     /translation="MDRLDWLRRAVILVFVPLGIGLIYLQVFSYGRFKNFSEGNSVRT
                     LYLDIPRGKILDRNGEILAEDKLGFNLTFVPFDLSNPKRCADILAPILQQSRSDLLQI
                     FSARYPNPFEIQVLARGLSQEQLHFVEEQLAQLGGIYIQAGLERRYPLGEHAATIVGY
                     LGEVNRDELASLRNEGVRSGDLLGRAGIERIFDALLRGERGGTSVVVDARGHQIRILG
                     QKESKPGNSIVLTIDAGLQRAASEKLGDRPGSVVILAPATGEVLALVSKPSYDPYRPA
                     AYLFSKSKPMLNRALTGLYAPGSVFKIITAIAALEEGAITPDDQIECTGVITLGEREF
                     HCWKEEGHGAISFKSALAQSCNIFFGEAGMKTGESALLDYALRAGLGVPTGIDLPGEK
                     SGSIPEPGESGGALNLSIGQGALLLTPVQMASLIATVANGGNIMRPEVIRELRTPDGT
                     LVEKYGPALRGSFFVSEGTLKLLREALRAVVDIGTGHAAAVPGIPVAGKTGTAQTAQR
                     DLNQPTRGSFVCYAPADAPELAVAVVLDSGASSEAAVIAGQIMRNYFLQAREAGGEDT
                     AVKSSSEPTED"
     gene            4278..5366
                     /gene="rodA"
                     /locus_tag="PKN80_04460"
     CDS             4278..5366
                     /gene="rodA"
                     /locus_tag="PKN80_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNQ35299.1"
                     /translation="MINRERLYLPVLVLTGVLLFIGLVLIFSSSRVIGKDLNDLFLKQ
                     LIWLAIGLAFLILLAGTDFHFWTRNAPFFYLLILLLLAAVLFIGHGRSGTQRWFRLAG
                     FNFQPSEFGKLFFIFTLAAYLEWRKDAVKRFGTLFFAALFILLPPFLLVLLQPNLGTA
                     FIMIPIFLVMFYAAGSPRKHLVTLVLTGCCALPFLWLFLKDYQKQRILVFLNPSADPL
                     GAGYNILQSRIAIGSGGIFGKGFLKGTQTHLAFLPEHHTDFIFSVLAEEWGLLGSTVL
                     LALYLLFLIEIIRIASYTRDRAGTSVAVGIMAMFATQVFINIAMTMGFLPVVGIPLPF
                     LSYGGSSLIASLSAVGILLNIKQQETFI"
     gene            5394..7046
                     /locus_tag="PKN80_04465"
     CDS             5394..7046
                     /locus_tag="PKN80_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNQ35300.1"
                     /translation="MRFLNLIPRPGRYVGAEVNAAGRDWNSARVRLCLCFPDTYEVGM
                     SNLGLRILYGTVNNAGSYLAERVFIPDPEAEAGFRQHHEGLWSLESRHSLRDFDLIGV
                     GLASELSYTNLLTLLSLAGLPRLSKDRTETDPIVLAGGNGALTPEPLAPFVDAFFMGE
                     ADTAILGIMSALERIRGGRRREKLLELARLEGVYVPAFYRPEYDADGDYLGLTGLETS
                     ASPRIRRVTVSDLEAVPFPTDWLVPYLPVIHDRVTLEIMRGCVHRCLFCQARVIYGPC
                     RVRSPEKLVDLADRALLKTGYEEISLLSLSSGDYPRLKKLLAGLRFVCEKHRARFSFP
                     SLRVDSLDLEQVRTEFGRKPPALTFAPESSEACRFRLGKRITDAQILEKAAAASQAGW
                     RHLKLYFMLGLPGEEESDLRAIVVLIRELSRRLAVNVSFNTFIPKPYTPLERADLINR
                     EAFEVRSQILRRELRGVRSLKLDFHAYEQSRLEALLCRGDRRVGRVIEEAWENGCRFD
                     AYREHFHPELWEKAWLDCGLDPDRFLFKQSRRRVLPWNHIVI"
     gene            7148..>7711
                     /locus_tag="PKN80_04470"
     CDS             7148..>7711
                     /locus_tag="PKN80_04470"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNQ35301.1"
                     /translation="MRSSFMRKDYRFAPHRSLLKGLGYTDEELSRPLIGVANSGNELV
                     PGHMYLDRVAEAVKQGIYLAGGTPFEFRTIGICDGIAMGHPGMRYSLCSRELVADSVE
                     VMAQAYPFDGLVLIPNCDKVVPGMLMALLRVNIPGLLVSGGPMLAGNFQGRKVDLISV
                     FEAVGGRAAGRVSDEELFTRLLYYGLAH"
BASE COUNT         1424 a         2235 c         2304 g         1748 t
ORIGIN      
        1 actgagcagg tctccgcggt tggccggtat ttcgagatcg aaaatccagt cgccgtcggc
       61 ttcgaacaga ttttccgaat ggagcccgat tcgggaaagg ttcttgagca ggtcggaaac
      121 cggcagatcg tcgagcggga tcaattcctt caaccaattc agggaatagc gcattgactt
      181 ttatcggttt ttgatagtaa aatatcggtt cggattctgt ttttacccgg ccattccaaa
      241 acagcttaaa ttataacata aaaggagtta ggatgggcaa gttttttaat ttccgttttt
      301 cgaccgacat cggcatcgat ctgggcacgg ccaacagcgt tgtttacgtc cggggcaagg
      361 gcatcgtgct ttccgaacca tcgatcgtgg ccattgaccg cgagacgcac aagatgatcg
      421 ccgttggcaa ggaagccaag aagatgcttg gccggacgcc gaccaacatc atcgccatgc
      481 gcccgatgcg cgacggggtg atcgctgatt ttgaggcgac cgaggcgatg ctgcgccact
      541 ttctggatca ggtccgcagt acccgccacc gctttttttt gccgccccgg gtggtgatcg
      601 ccattccttc cgggattacc agcgttgaaa agcgggcggt catcgagacc gcgaccaagg
      661 ccggtgcccg gtcggtttac ctgattgaag aaccgaaggc ggccgccatc ggcatcggac
      721 tgccggtcgg ggaacccatc ggcaacttca tcatcgacat cggcggcggt accaccgaga
      781 tggcggtcat ctctctgggg gacatcgttt tgccgcgcag cataaggatc ggcggtaacg
      841 agatggatga gtcgataacc gagtacctga agaaaaagta caaccttctt atcggagaaa
      901 ggacggctga ggagatcaaa attcagatcg ggtcggcctg gcccctggaa gaggaaatga
      961 gcctggacgt gaccggccgg gatctggtgg aggggttgcc gcgccgtata acggtgcggt
     1021 cggaggagat ccgggagtca cttcaggaaa accttacggc gattatcaac gaggtgcgcg
     1081 ccctcctgga gaatactccg cccgaactgt ccagcgatct ggcggtgcgc ggtatttaca
     1141 tggccggcgg cgggtcgctg ctgcgcggta tcgaccggct gctttcttcc gaaaccaaga
     1201 ttccggtcca ggttgccgac gaaccgctgc tggcggtggt gcgcggtacc ggaaggatgt
     1261 tggaggaaga gaagtatttt accaagatac agccctacca gacctgaccg gattctccgc
     1321 ccggcagcgg cggccggttt tccagccggt ccgattgacc tcggaccgga taataacttg
     1381 acttcaaccc gatatgctct ggaggtttca aaaagaaatc ctgctggtcg tttttcttct
     1441 gatttcgttc acgctgacca atctcaagat aagcggggga cgaacgggcg accggacttt
     1501 tttgaagctg gaagagtatc acctgcagtt tgaacaggaa gtgcttcggg aaaaccagcg
     1561 cctgcgccgg ctgctgggca tgaaagaacg ccgggcgaac cggatcctgg ccattggcgg
     1621 ggtggtgggc atcgatccga tgggttcgcc cagctggctg ttggcggaag tcggaaaccc
     1681 ggaggcactt cggcccgatc tggccgccct cgacggccgg ggaaacctgg tcggccgggt
     1741 ggtggaaatc aaggggcgca ccctcagggt aatgaccatc ctgaaccccc agagccgggt
     1801 cagcgtcgtc ctccaggaga gccgaaacct gggtgtcctg gaatccgggg aggcgggcag
     1861 tctcaagatc aattacattc cccgcgaggc gacggtcctg gagaaggagg cggtgatgac
     1921 ctcccagctg agccataatt tcccggccgg gatcccgatt ggccgggtcc agcggattgc
     1981 caagaaagga ttttttaccg aggtgaccgt tctgcccaac gccaattttt caacgctgga
     2041 agaggtggcg tttgcccatt aatccgcgca acttcatttt cgtgatcctg gccaatttcg
     2101 gggttcagta cctggccggc cgatatctga ttattttcga taccattccc gacctgaacc
     2161 tgctgctggt actttatctg agctttcact ttgtggacgc caccggtttg atggccgctt
     2221 tcgcgctcgg gatttttcac gaccttgttt ttcgatacca gctgggcagt cacagcctgg
     2281 tttacgtagc catcgcctac ctggccaacc gggccgttcc ggggcgattt gaactgacgc
     2341 cggcgaaaag tttcatcctt tgtttttact ttgcctttct gtttgccatc ggttacgaaa
     2401 tgttgctgcc gcccgcctgg ggggcgggta ttttctggaa gcactctctc tttgctttct
     2461 acagcagcgc ctggtcgctg ccggcttttt tcctttacga gctgggtttt caacaaaccc
     2521 gggaaccggt tttctaggat ggacagactc gactggctgc gacgggcggt gatcctggtc
     2581 ttcgtgccgc tgggaatcgg gcttatctac ctccaggttt tttcctacgg acgcttcaag
     2641 aatttctcgg aagggaattc ggtccggact ctctaccttg acataccgcg cggcaagatc
     2701 ctggaccgca acggagaaat cctggcggaa gacaagcttg gcttcaacct taccttcgtg
     2761 ccgtttgatc tttccaatcc gaaacggtgc gccgatatcc tggccccgat cctccagcaa
     2821 tcccgttccg acctgctcca gattttcagc gcccgttacc ccaacccatt cgagatccag
     2881 gtgctggccc gcggtttatc ccaggaacag ctccacttcg tggaagaaca gcttgcccag
     2941 ctgggaggga tttatatcca ggccggattg gaacgccgct atcccctggg cgagcatgcc
     3001 gccaccattg tcgggtatct gggcgaggtt aaccgggatg aactggcctc gcttcgcaac
     3061 gaaggcgtac gttccggaga cctgctgggg cgggccggga tcgagcggat ttttgacgcg
     3121 ctcctgcgcg gagaacgagg cgggaccagc gtggtggttg atgcccgcgg ccaccagatt
     3181 cggattctcg ggcagaagga gtcgaaaccg ggcaacagca tagtgctcac catcgatgcc
     3241 ggtctgcagc gggccgcttc cgaaaagctg ggcgaccggc ccggttcggt ggtcattctg
     3301 gccccggcca ccggcgaagt cctggctctg gtaagcaagc cgagctatga tccctaccgt
     3361 ccggccgcct atctcttttc caagtccaag ccgatgctga accgggcctt gaccggactt
     3421 tacgccccgg gttcggtctt caagatcata accgcgatcg ccgcccttga ggagggggcg
     3481 atcacgcccg atgaccagat tgaatgcacc ggcgtgatca cgcttggcga acgcgagttt
     3541 cattgctgga aagaagaagg acacggcgcc atctcgttca agtcggccct ggcccaatcc
     3601 tgcaacatat tctttggcga ggccgggatg aagaccggag aatcggctct gcttgattat
     3661 gcgctgcggg ccggacttgg cgttccgacc ggcatcgacc tgccgggtga aaaaagcggt
     3721 tccatccccg agccgggaga atcgggcggg gccctgaacc tctctatcgg ccagggggcc
     3781 ctgctgctta ctcccgtgca gatggccagc ctgatcgcca ccgtggccaa cggcggcaat
     3841 atcatgcgtc cggaagtgat ccgggagttg cgcactccgg atgggacgct ggttgagaag
     3901 tatggaccgg ccttgcgggg ttcctttttt gtttccgaag gaactctcaa gctgcttcgg
     3961 gaggcgctgc gggcggtggt cgatatcggg accggccacg cggccgccgt tccggggata
     4021 ccggtggccg gcaagaccgg caccgcccag acggcccagc gcgatctcaa ccagcccacc
     4081 cgcggcagtt ttgtctgtta cgcgccggcc gacgctccgg aactggcggt ggcggtggtt
     4141 ctcgacagcg gggcctcctc ggaggcggcc gtcatcgccg gccagattat gagaaattat
     4201 tttctgcaag cccgggaggc cggcggcgaa gacaccgcgg ttaagagttc ctccgagcca
     4261 acggaagact gacgctcatg atcaatcgag agagacttta tctgcccgtg ctggtcctga
     4321 ccggagtgct gctttttatc gggttggtgc ttatcttcag ttccagccgg gtgatcggca
     4381 aggacctgaa cgaccttttt cttaagcagc tgatctggct ggccatcggg ctggcctttc
     4441 tgatcctgct ggccgggacc gattttcatt tctggacaag aaacgcgccc ttcttctacc
     4501 tgttgatact gcttctgctg gcggcggttc tttttatcgg gcacggccgg tccggaaccc
     4561 agcgctggtt tcggctggcc ggatttaact tccagccctc ggaattcggc aagctctttt
     4621 tcatattcac gctggccgcc taccttgagt ggcggaagga tgccgtaaag cgtttcggca
     4681 ccctgttttt cgccgccctg tttattttac tgcccccgtt tctgctggtc ctgctgcagc
     4741 caaacctggg gaccgctttc atcatgatac ccatctttct ggtgatgttt tacgcggccg
     4801 gttcgcccag aaagcacctg gtgacgctgg ttttaaccgg ttgctgcgcc cttcccttcc
     4861 tctggctttt cctcaaggac taccagaagc agcgcatcct ggtctttttg aatccgagcg
     4921 ccgatcccct gggcgccggc tataatattc tccagtcaag gatagccatc ggctcgggag
     4981 ggattttcgg caagggattt ttaaagggca cccagacaca cctggccttt ctgccggagc
     5041 accataccga cttcatcttt tcggtgctgg ccgaggaatg gggtctgctc ggcagcaccg
     5101 ttctgctggc cctttacctg ctctttctga tcgagatcat ccggattgcc tcttacaccc
     5161 gggaccgggc cggaacttcc gtagccgtag gcatcatggc catgtttgcc acccaggttt
     5221 tcattaatat cgccatgacc atgggcttcc tgccggtggt cggcatcccc ctgcccttcc
     5281 tcagttacgg cgggtcctcc ttgatcgcct cgctttcggc ggtcgggatc ctgctgaaca
     5341 tcaaacagca ggaaaccttc atctgaatgg cggtttccca ttccgaagct gcgatgcgtt
     5401 ttctcaatct gattccccgc cccgggcgct acgtgggcgc cgaagtcaac gccgccggcc
     5461 gcgactggaa ctcggcccgg gttcgtcttt gtctctgctt tcccgacacc tacgaggtcg
     5521 ggatgtccaa ccttggtttg cgcattctct acgggacggt caacaacgcc ggttcctacc
     5581 tggccgaacg cgtcttcata cccgatccgg aggcggaggc cggattccgg cagcaccatg
     5641 aaggcctctg gagtcttgaa tcacgccatt cgcttcgtga tttcgacctg atcggggtgg
     5701 ggctggccag cgaactttca tacaccaacc tgctgacgct cctgtcgctg gccggactgc
     5761 cccgattgag caaagatcgg accgaaactg acccgatcgt cctggccgga ggtaacggcg
     5821 ccctgacccc ggaaccactc gcccctttcg tggacgcctt ttttatgggc gaagccgaca
     5881 cggccattct ggggatcatg tcggccctgg aacgcattcg cggcggccgc cggcgtgaaa
     5941 aactccttga gctggcccgc ctggaagggg tttatgtgcc ggccttctac cgcccggaat
     6001 acgatgcgga tggagattac ctcggattga ccggcctgga aacgtccgct tcaccccgga
     6061 ttcggcgggt aacggtttcc gatctcgagg cggttccatt tccaacggat tggctggttc
     6121 cctacctgcc ggtgatccac gaccgggtta ccctcgagat aatgcgggga tgtgtgcacc
     6181 ggtgcctctt ctgccaggcc cgggttattt acgggccctg ccgggtgcgc agcccggaaa
     6241 aattggttga cctggccgac cgggccctgc tgaaaaccgg ttacgaggaa atctccctgc
     6301 tctcgctttc ctcgggcgat tatccccggc ttaagaagtt gctggccggc ctccggtttg
     6361 tctgcgaaaa acaccgggcc cgcttctcat ttccttcgct ccgggtcgat tcgcttgatc
     6421 tggaacaggt ccgcacggag ttcggccgca agcccccggc cctgactttc gcccccgaga
     6481 gttccgaggc gtgccgtttc cggctgggta aacggataac cgacgcccag attctggaaa
     6541 aggcggcggc ggcttcgcaa gccgggtggc gccatttgaa actttacttt atgctggggc
     6601 tgcccgggga ggaagagtcc gatttgaggg cgatcgtcgt tcttatccgc gaactttccc
     6661 gccggctggc ggtaaacgtt tcattcaata cctttattcc caagccatac actccactgg
     6721 aacgggccga cttgatcaac cgggaggcct ttgaagtccg gtcacaaatc ctgcgccggg
     6781 aattaagggg tgttcgaagt ctgaaactgg attttcatgc ctacgagcag agccgtctgg
     6841 aggccttgct ttgtcggggc gaccgccggg tcggccgggt tatcgaagaa gcctgggaga
     6901 acggttgccg ttttgacgcc taccgggaac actttcatcc ggaactttgg gagaaggcct
     6961 ggctggattg cggccttgat cccgatcgct ttctgttcaa gcagtcccgt cgccgggttc
     7021 taccctggaa tcatattgtg atataataca aaataaaacg ccgtcaatca taaatccgcc
     7081 gggattaccc ggggtgtcaa gcgtctggtt gtccggttga tcgggagggc gattttcagg
     7141 aggagacatg cgcagcagtt tcatgaggaa ggattatcga tttgcgccgc accgctccct
     7201 tttgaaggga ttgggttata ccgacgagga attgtcccgg ccgctcatcg gcgtggccaa
     7261 ttccggcaat gaactggtgc cgggacacat gtatctggat cgggtggccg aggcggtcaa
     7321 acagggcatc tatctggccg gggggacccc tttcgaattc cggacgatcg gcatctgcga
     7381 cgggatcgcc atgggacacc cgggcatgcg ctactccctt tgctcccgcg agctggtggc
     7441 cgacagcgtt gaagtcatgg cccaggccta tccttttgac gggctggtgt tgattcccaa
     7501 ttgcgacaag gttgttcccg gtatgctcat ggccctgctg cgggttaaca tccccggcct
     7561 tcttgtcagc ggggggccga tgctggccgg gaattttcag gggcgcaagg ttgacctgat
     7621 ctcggtcttc gaggcggtgg gaggccgggc ggccggccgg gtaagcgacg aggagttgtt
     7681 tactcgtctt ttgtattacg gcttggccca t
//
LOCUS       DAOQYN010000082         7674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9936_length_7674_cov_5.042525, whole genome shotgun sequence.
ACCESSION   DAOQYN010000082 DAOQYN010000000
VERSION     DAOQYN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9936_length_7674_cov_5.042525"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKN80_04475"
     CDS             complement(<1..816)
                     /locus_tag="PKN80_04475"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNQ35302.1"
                     /translation="MKTPKQENPFEMACRQLDECAGRLQLSRGAHQLLRTPLREYHFT
                     MPVRMDNGDIEVFHGFRIQYNNARGPTKGGIRFHPEETIDTVRALAAWMTWKCALLDL
                     PLGGAKGGVVCDPRKLSVCELERVSRAYIQAVWPVIGPHRDIPAPDVYTNSQVMSWMM
                     DEYSRITGEALGGVITGKPMSLGGSVGRDDATARGGLYTIREAAREIGLDLKGATVAV
                     QGYGNAGYHIARLLRRHFNARIVAISDSRGAIKNPRGLDPEKVLAYKQKKGSVA"
     gene            complement(880..2634)
                     /gene="aspS"
                     /locus_tag="PKN80_04480"
     CDS             complement(880..2634)
                     /gene="aspS"
                     /locus_tag="PKN80_04480"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNQ35303.1"
                     /translation="MMRSHNCGELSAGDLDREVRLAGWVGARRDHGGIVFINLRDRYG
                     VTQVVFNPERLSRDDFEKSAALHSEDVIQVRGRVTRRPAGTENPRMSTGEIEVLAEGL
                     VLLNRCAPIPFEIDEAAQVSETVRLGYRYLDLRRPVMQRNLILRHRAARTAREFLSEK
                     GFVEVETPLLTRSTPEGARDFLVPSRLDPGKFYALPQSPQLSKQLLMVSGLDRYFQLA
                     RCLRDEDLRADRQPEHTQIDLEASFVEPEDIQDLVEEMVARIFQTVNGRPLERPFPRL
                     TYQQAVDVYGSDKPDRRFGLEITDVTELAAAGGFEVFRKAVAAGSRIKGLALPGVDPS
                     VTQLKGYEEKVRELGARGLVWIKAAGGNFQSPVAKFLGPEILAGLAGKFGLEKGVIFM
                     VADADRKASLLLGAFRLMLASDFNLVPPDRFCFLWVVDFPLFEHDEESKRLTSVHHPF
                     TAPRPEDLDYLESEPLRVRSQAYDLVLNGTEIGGGSIRIHQAELQRRIFKILNLEPAA
                     WEREFGFLLAALERGAPPHGGLALGFDRLLTTFLGLPSIREVIAFPKTQKGACLMTGA
                     PSEVSPEQLKDLDLRRIS"
     gene            complement(2618..3877)
                     /gene="hisS"
                     /locus_tag="PKN80_04485"
     CDS             complement(2618..3877)
                     /gene="hisS"
                     /locus_tag="PKN80_04485"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNQ35304.1"
                     /translation="MEKRYQAVRGTYDLLPAAARKLAAFLKLVTGRLEQAGFQELETP
                     VLEEARLFNRSVGPDSDLVTKEMYTFQDRKGRLLALRPEGTAPLVRAYLEHDFTYHAP
                     QARFYYLGPMFRYDRPQEGRYRQFLQLGGELFGVAGVQAESETLDLLNRVFNLAGLPE
                     FRFEVNSLGCRACQEEYGRRLSEFIEPRAGGLCPDCLRRSKQNPLRVLDCKVDACRKI
                     LGQAPVVGGFLCPACREHHRLFLECLRLLEVPFLENPRLVRGLDYYTRSVFEVTLAGG
                     GNALAAGGRYDNLVEELGGPATPAFGFAIGLERLMSVLPEPPAGRAGLRILYLEAAHL
                     PEALRLVRRFREALPVEWPVTVAPEARSLKSQLRQAARDGCRMVAVLGPEELARGEIL
                     WRDLESGRQVSLPPETMIERLKNDAQP"
     gene            complement(3986..4573)
                     /locus_tag="PKN80_04490"
     CDS             complement(3986..4573)
                     /locus_tag="PKN80_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35305.1"
                     /translation="MNLLKRKLYRILFGTFFALLAYFGGWGWSIACLGFLLVLLVGFE
                     YERRLHPGLYGWIAARSGGILKEQPGFFLGDTYFVLAVFFLFVLMPDLSLPLAGLLFL
                     TFGDAASTLAGSRFGRVQVYRRKTLEGFLACLAVTTILAGLLSRLPRHSLDFGVGFAG
                     AATASLAELFDLPPDDNFSVPVLSALIMLVLGFFV"
     gene            complement(4570..5406)
                     /locus_tag="PKN80_04495"
     CDS             complement(4570..5406)
                     /locus_tag="PKN80_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ35306.1"
                     /translation="MKKGPALRIKGLTAGYRNRPVLGGIDLELETGDFLGIIGPNGSG
                     KTTLLRALTGLVPHGGEIDWWEHPFHPSDSDWLARRVAVVSAGEDMPVAGRVEEYLWL
                     GRLPHRQFFQFWLDPGDRQLVDRISRQLGVESLRDRLLSELSSGERQRVAIARALVQQ
                     PRVLLLDEPTSHLDPGAQIEIMDLLAALNRNGLGIVAVLHDLNLAGAYCNRLLLMDGG
                     TARASGSPESVLTYRNLEAVYHTVVVTLANPVTGSPWVVPVPESYRRSSGPDSNVCKE
                     GE"
     gene            complement(5403..6386)
                     /locus_tag="PKN80_04500"
     CDS             complement(5403..6386)
                     /locus_tag="PKN80_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ35307.1"
                     /translation="MKTGTRAAVCAGLLLLLVAAVLAGLGIGTARIDLSALLRSGGLD
                     DYQRSIFFSLRLPRVAAALLAGGGLAVSGVLLQGLFRNPLVEPYTLGVSGGAALGVAL
                     AVLLPGGAAWRPWLAFGGAVVSVALVLAFSRRRHEFGVATTLLTGVMLSFVSSSLLML
                     IMALVRAEQLQGIIFWMMGSVGLSDPGLLRWSAPVFLAGVLWAWWLAFPLNGLALGES
                     AAHHLGLRVDRVQRSVFLAASLLTGLSVAISGIIGFVGLLVPHLLRPLLGSDHRWLIP
                     ASFMAGAAFLVGADLLSRTIISPLELPVGVITGLLGGSIFISSLYRRERHP"
     gene            complement(6383..7243)
                     /locus_tag="PKN80_04505"
     CDS             complement(6383..7243)
                     /locus_tag="PKN80_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNQ35308.1"
                     /translation="MIRHKSAVGPAAFLAAVCLGFFLSGSAAGSEPPRRIVSLAPSLT
                     ETLVDLGLGERIVGLTEQDNCRPVLKKAVTVGFYIQPNLEKIAALKPDLVLFDVYPGQ
                     AETRRRLAGLGLKVESFEVRRLSDILAVTDRLGGLTGRIREAGRLNRRLKAQLERYRN
                     PAGPGRPRVYLEVGHDPYFSAGRDSFLHELIELAGGRNIAGSLPGAYPRVDAEFIIAA
                     DPEVIILSGMDSGDARAVGRRPGWDRLSAVRSGRIHSGLGTHPLTTPSPRLILENLPV
                     LHRILNSARR"
     gene            complement(7286..>7674)
                     /locus_tag="PKN80_04510"
     CDS             complement(7286..>7674)
                     /locus_tag="PKN80_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35309.1"
                     /translation="SDNLQAADRIPAYQAARSFASAVNEYSLRRYQEKRAGVDIDRLQ
                     RNSSLIPAKIVSGHCLGYDQEFLRENIRLCREARAAVLDCEPILKRLSERPEETLDVT
                     LLLDLMRETILELNNWISNLERRVWW"
BASE COUNT         1576 a         2486 c         2403 g         1209 t
ORIGIN      
        1 ggctaccgac cctttcttct gcttgtaggc cagcaccttt tccggatcca ggccgcgcgg
       61 gtttttgatg gcgccccggc tgtcgctgat cgcgacgatc cgggcgttaa aatggcgccg
      121 gagcaggcgc gcgatgtgat acccggcgtt gccgtatccc tgaacggcca ccgtggcccc
      181 cttcaggtcc aggccgatct cccgggcggc ctcccggatg gtataaaggc cgccgcgggc
      241 ggtggcgtca tcgcggccaa ccgaaccgcc cagagacatc ggctttccgg tgatgacgcc
      301 gcccagcgcc tccccggtta tccggctgta ctcgtccatc atccaggaca tgacctggga
      361 attggtgtag acatcgggag ccggtatgtc ccggtgcgga ccgatcaccg gccagaccgc
      421 ctgaatataa gcccggctga cccgttccag ttcgcaaacc gagagttttc tcgggtcgca
      481 gaccacgcct cccttggcac cgcccagggg cagatcaagc agggcgcatt tccaggtcat
      541 ccaggccgcc agggcgcgca cggtatcgat cgtctcttcg ggatggaacc tgatgcctcc
      601 cttcgtcggc ccccgggcgt tgttatactg aatccggaat ccgtgaaaaa cctcgatatc
      661 gccgttgtcc atccggaccg gcatggtaaa gtgatattcg cgcaggggtg tccgcagcaa
      721 ctggtgcgcc ccccgggaca gctgcaggcg gccggcgcat tcgtccagct ggcggcaggc
      781 catttcaaag ggattctctt gtttcggcgt tttcatattc cactccggtc cggcggtaag
      841 gaaaaggctt gatccgtacg aatccgcgtc ggggtcggct caggaaatcc ggcgcaggtc
      901 gaggtctttc agctgttccg gcgacacttc cgagggcgcc ccggtcatga gacaggcgcc
      961 cttctgggtc ttggggaaag caatcacttc gcggatggaa ggcaatccca gaaaagtggt
     1021 cagcaagcgg tcaaaaccaa gggccagtcc gccgtgggga ggcgccccgc gctccagggc
     1081 ggccaggaga aagccgaact cccgctccca ggcggcgggt tcaagattca ggatcttgaa
     1141 tatccggcgc tgcagttcag cctggtggat ccggatgctg ccgccgccga tttcggttcc
     1201 gttaagcacc aggtcgtagg cctgactgcg gacccggagc ggttcgcttt cgaggtagtc
     1261 caggtcctcg ggccggggtg cggtaaacgg gtggtgtacc gaagtcaggc gtttcgattc
     1321 ctcgtcgtgt tcaaagagcg ggaagtccac cacccagagg aagcagaaac gatccggcgg
     1381 gaccaggttg aaatccgagg ccagcatcag gcggaacgct ccgagcagga ggctcgcctt
     1441 ccggtcggcg tcggctacca tgaaaattac gcccttctca agtccgaatt ttccggccag
     1501 cccggccagg atttccggcc ccaggaactt ggccaccgga gattggaaat tgccgccggc
     1561 ggccttgatc cataccagtc cgcgggcgcc cagttcccga accttctcct cgtatccctt
     1621 aagctgggta accgacggat ccacccccgg cagggccagt cctttgatac ggctgccggc
     1681 ggcaaccgcc ttgcggaata cctcgaaccc gccggcggcc gccagctcgg tgacatcggt
     1741 tatttcaagg ccgaaacgcc ggtcgggctt gtcggaaccg taaacatcca cggcctgctg
     1801 ataggtcaag cgcggaaacg gccgctccag gggacggccg ttaaccgtct gaaaaatccg
     1861 ggccaccatc tcttcgacca ggtcctggat gtcctccggc tccacgaaag aggcctcaag
     1921 atcaatctgg gtgtgttccg gctggcggtc cgcccgcaga tcttcgtcac gcaggcagcg
     1981 ggccagctgg aaataacggt ccagcccgga aaccatcagc agctgcttcg aaagctgggg
     2041 gctctgcggc agggcgtaaa acttgccggg atcaagccgg ctgggcacca ggaagtcccg
     2101 ggctccctcc ggggtgctgc gggtgagcaa gggggtttcc acctccacaa aacctttttc
     2161 actcagaaat tcccgggccg ttcgggccgc ccggtggcgc agaatcaggt tccgctgcat
     2221 taccggccgg cgcaaatcaa ggtagcggta accgagccgg accgtttcag aaacctgggc
     2281 cgcctcgtca atctcgaaag gaatcggggc gcagcgattg agcagaacca gtccctcggc
     2341 cagcacctca atttcaccgg tggacatccg gggattctcg gtcccggccg gacgccgggt
     2401 cacccggccc cgaacctgga tgacatcctc gctgtgcagc gccgccgatt tttcaaaatc
     2461 atcccgggaa agccgctcgg gattaaaaac aacctgggtc accccgtaac ggtcgcgcag
     2521 gttgatgaag acgattccgc cgtggtcccg gcgcgctcca acccagccgg ccaaccggac
     2581 ctcccggtcc agatcgccgg cggataattc cccgcagtta tggctgcgca tcatttttaa
     2641 gcctttcaat catggtttcc gggggcagcg aaacctggcg gcccgattcc aggtcgcgcc
     2701 agaggatctc accccgggcc agttcttccg gaccaagcac ggccaccatc cggcagccgt
     2761 cccgggcggc ctggcgcagt tgggatttca ggctgcgggc ctcgggagca accgtaaccg
     2821 gccattcaac cgggagcgcc tcccgaaaac gacggaccag ccgcagcgcc tccggcaggt
     2881 gggccgcttc caggtaaaga atccgcagtc cggcccggcc ggccgggggc tccggcagga
     2941 cggacatcaa gcgttccagc ccgatcgcga aaccaaaagc cggggtggcc ggaccgccca
     3001 attcctcaac caggttatcg taacggccgc cggcggccag ggcgtttcca cccccggcca
     3061 gggttacctc aaaaacggaa cgggtataat agtcaagacc gcgcaccagg cggggatttt
     3121 ccagaaaagg cacctccaga aggcgcaggc attcgagaaa aagacggtgg tgctcccggc
     3181 aggccggaca gaggaaaccg ccgacaaccg gcgcctggcc caggattttc cggcaggcgt
     3241 ccaccttgca atcgagaacc cgcagcgggt tctgcttcga ccgtctcagg caatccggac
     3301 agagaccccc ggcgcgcggc tcaataaatt ccgaaaggcg ccggccgtac tcctcctggc
     3361 aggcccggca accgagggaa ttgacttcaa accggaactc cggaagccca gccagattga
     3421 agacacggtt caacaagtcc agcgtttccg actcagcctg gaccccggct actccaaaaa
     3481 gttctccccc gagctgcaga aactgtcggt agcggccctc ctggggacga tcgtaacgaa
     3541 acatcggccc caggtaataa aatcgggcct ggggcgcgtg ataggtgaaa tcatgttcga
     3601 ggtaggcacg gaccagggga gcggttccct ccggccgcag ggccagcagc cgtcccttgc
     3661 ggtcctggaa agtatacatc tccttggtaa ccaggtcgct gtcgggcccc acgctgcggt
     3721 tgaaaaggcg ggcctcttca agaacgggtg tctccagttc ctgaaatccg gcctgttcca
     3781 ggcgcccggt gaccagtttc aaaaaggcgg ccagtttccg ggccgccgcc ggcagcaggt
     3841 cataggtgcc gcggactgcc tgatagcgtt tttccataat gccgttttcc cggggccgga
     3901 accggacccg cccggagacc tcaataatga cgccgaaatt tggggaggga aattgtaaaa
     3961 ttatatattt taaagccggc cggtgtcaaa caaaaaagcc cagcaccagc atgatcaacg
     4021 ccgataaaac cggcacgctg aagttatcgt cgggcggcag gtcaaaaagt tccgccaggg
     4081 aggcggtggc ggcgccggcg aatcccacgc cgaaatccag agagtggcgg ggcagccggg
     4141 aaaggagtcc ggccaggatc gtggtgacgg ccaggcaggc caggaatccc tccagcgtct
     4201 tgcgccggta aacctggacc cggccgaagc ggctgccggc cagggtgctc gccgcatcgc
     4261 cgaacgtcag aaaaagcagg cccgccaggg gaaggctcaa atccggcatc aggacaaaaa
     4321 gaaagaagac ggccagaacg aaataagtat cgcccagaaa gaagcccggc tgttccttga
     4381 ggatgccgcc gcttcgggcg gcgatccagc cgtaaaggcc gggatggaga cgccgctcgt
     4441 attcgaaccc gaccagcagg accagaagga aacccaggca ggcgattgac cagccccagc
     4501 cgccgaagta ggccagcagg gcgaagaaag tcccgaaaag aatccggtat agcttgcgtt
     4561 tcaaaaggtt cattccccct ccttgcagac attggaatcc ggaccggacg aacgccggta
     4621 cgactccggg accgggacca cccagggact cccggtcacc ggattggcaa gagtgacgac
     4681 cacggtgtga tagaccgctt ccagattccg gtaggtcagc accgactccg ggcttccgga
     4741 ggcccgagcc gtccccccat ccatcagcag taaccggttg caataagccc cggccagatt
     4801 caagtcgtgc aggaccgcca cgatgccgag cccgttccga ttcagggcgg ccagcaggtc
     4861 catgatctca atctgggcgc ccggatccag gtggctggtg ggttcgtcca gcaggagcac
     4921 ccgcggctgc tggaccaggg cccgggcgat cgcaacccgc tgccgttcac cgctggatag
     4981 ttccgaaagc aggcggtccc ggagggactc cacgccgagc tgccggctta tccgatccac
     5041 cagttgacgg tcgccgggat cgagccagaa ctgaaaaaat tgacggtgcg gcaaccgtcc
     5101 cagccagaga tactcctcga cgcggccggc aaccggcatg tcctccccgg cgctcaccac
     5161 cgccacccgg cgggccagcc aatccgaatc ggacggatga aaaggatgtt cccaccaatc
     5221 tatttcgccc ccgtgcggaa ccaggccggt gagagcccgc aacagggtgg ttttcccgct
     5281 gccgttgggg ccgatgatcc cgagaaaatc gcccgtttcc agttccaggt caatcccgcc
     5341 caacaccggc cggttgcggt agccggccgt caggcccttg attctcaagg ccggtccttt
     5401 cttcatgggt gacgctcccg gcggtaaagc gaactgataa agatgctccc gcccagcagc
     5461 ccggtaatta cacccaccgg caactccagg gggctgatga tcgtccggct taaaagatcg
     5521 gctccgacca ggaaggcggc gcccgccatg aaggaggccg gaatcagcca ccggtgatcc
     5581 gaccccagga gcggacgcag gaggtgggga accagcagcc cgacaaaccc gatgatcccg
     5641 ctgatggcca ccgaaagacc ggtcaaaagc gaggcggcca ggaagaccga ccgctggacg
     5701 cggtcaaccc ttaagccgag atggtgggcg gccgattccc ccagcgccag tccattcagc
     5761 ggaaaggcca gccaccaggc ccagagaact ccggccagga agaccggcgc actccagcgc
     5821 agcagtcccg gatcgctcaa gccaacgctg cccatcatcc agaagatgat tccctgcagc
     5881 tgctcggctc gcaccagggc cataatcagc atcaggagcg aggaggaaac aaaactgagc
     5941 atgaccccgg tcagcagtgt cgtcgccacg ccgaattcgt gccggcggcg gctgaaagca
     6001 agcaccaggg cgacgctgac aaccgcgccg ccgaaggcca gccagggacg ccaggccgca
     6061 ccacccggca ggaggaccgc cagggcgacc cccagggcgg ccccgccgga gacgcccagg
     6121 gtatagggtt ccaccagcgg gttgcgaaat aacccctgga gcagcacgcc gctgaccgcc
     6181 aggccgccgc cggccagcag ggcggccgcc acccggggca gccggagcga aaagaagatg
     6241 gaccgctggt agtcgtccag gccgccggac cgcaacaagg cggacaggtc gatccgggcc
     6301 gtaccgattc ccagcccggc caggaccgcc gccaccagca gcagtaacaa tccggcgcaa
     6361 acggccgctc tggtgccggt cttcatcgcc gggcggaatt caggatgcga tgcaacacgg
     6421 gaaggttttc caggatgagc cggggcgagg gggtggtgag cggatgggtg ccgagcccgg
     6481 aatggattct ccctgaccgg acggcgctca accgatccca accgggccga cgccctaccg
     6541 cccgggcatc cccggaatcc atgccgctca aaatgatcac ctccggatcg gccgcaatga
     6601 tgaactcggc atcaaccctg ggataggcgc cgggcaggct cccggcgatg ttccggccgc
     6661 cggccagctc gatcagttca tgcaggaagg aatcgcggcc ggccgaaaaa taagggtcat
     6721 gaccgacctc caggtaaacg cgggggcgcc cggggccggc cggattccgg taacgttcca
     6781 gttgggcctt cagccgccgg ttaaggcggc cggcctcgcg gatccggccg gtcagtccgc
     6841 ccaggcggtc ggtaacggcc aggatgtccg agaggcggcg gacctcgaag gactccacct
     6901 tgaggccgag tccggccagg cgacgcctcg tttcggcctg gccgggatag acgtcgaaga
     6961 gaaccagatc gggtttgagg gcggcgatct tttccaggtt gggctgaatg taaaaaccga
     7021 cggtcaccgc ttttttcaaa accggccggc agttatcctg ctcggtcagc ccgacaatcc
     7081 gctctcccag gccgagatcc acgagggtct cagtcaggga gggagccagg gaaacaatcc
     7141 ggcgcggcgg ctcggaaccg gcggccgatc cggacagaaa aaaaccgagg cagacggccg
     7201 ccaggaacgc cgccggaccg accgccgatt tatgcctgat catttgaaat gtccgcggga
     7261 cgcgggggtg gccggtagga agatttcacc accagacgcg cctttccaga ttcgagatcc
     7321 agttgttcaa ttccaggatc gtttcgcgca tcaggtccag gagcagcgtt acatccaggg
     7381 tctcctccgg ccgttcggaa agacgcttca aaatcggttc gcagtccaat acggccgccc
     7441 gcgcctcgcg gcaaagccgg atgttctccc ggaggaattc ctggtcgtac ccgaggcagt
     7501 gcccgctgac gatcttggcc ggaatcaggc tggaattgcg ctgcagccgg tcgatgtcga
     7561 ccccggcccg tttctcttgg taacggcgca ggctgtattc gttgaccgct gaggcaaagg
     7621 aacgggcggc ctggtaggcc ggaatgcggt cggcggcctg gaggttgtcg gaat
//
LOCUS       DAOQYN010000083         7652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_9974_length_7652_cov_4.905752, whole genome shotgun sequence.
ACCESSION   DAOQYN010000083 DAOQYN010000000
VERSION     DAOQYN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9974_length_7652_cov_4.905752"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="PKN80_04515"
     CDS             <1..1364
                     /locus_tag="PKN80_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35310.1"
                     /translation="SYLPVFLKTPFYYFDGSGPVATALNEMLLQGWNGVIRVCPALPE
                     DWEAKFTLLTPGGFLISGQAAGGQVKYLSILSQRGETAAVANPFGRPAAVNDGRRVIL
                     RSAAPELKFPTEAGREYRIYPADQNEPRPSMVTGETNQGPKTFPESSRRLGKSSFEIP
                     ELAGRLEKRPAGRDFEPDWTQALKVARTAGPVRIDGRLTEACWRGAPAGTLRLAGGRR
                     PGQRTDVWLAADDQALYVAAEAREDRMKMVQAERGVPGDPLEPRVALDDSLALIMEPM
                     PGAQWRLRVNPLGVQNDSLFVAGVRPPLEERLSLMDPEWESSIRTTEDGWIMEARLEY
                     ARFAPFPPGTGQTWRFGVERNEYPHGERTAWPSETTGGAKELARLELPAREAKAAVIE
                     PQPDLQLHWKLEAVDGPWVLDASGHARHGYFSGARSGRETPDSVLRFAGGTPDEKLKF
                     NAG"
     gene            1377..1799
                     /locus_tag="PKN80_04520"
     CDS             1377..1799
                     /locus_tag="PKN80_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNQ35311.1"
                     /translation="MGSGLAESRELTAAAWINPASSKSMRILEKPGNEFIFDILAGKL
                     RLISTAGTFGGTAAVPAGRWSHVAVTIAPGTLRLFLNGEPCGEARSGRPLTLSAGPLL
                     IGTTPDGRYSFNGSLADLRLYNRALTPAEISTLHAGGR"
     gene            1916..5128
                     /locus_tag="PKN80_04525"
     CDS             1916..5128
                     /locus_tag="PKN80_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35312.1"
                     /translation="MKYGMLVLSWLTLAVLTTSFSSAEAADEGTIGPWRVGDMAYSKT
                     PANDRPNAESQRIVERNYTALVSEKLLTGEKGKLTFRVAPRNERWAGILVMDNDGKPL
                     PYNLKRGAKVSFAEAGTGKTDPAVRWYQYDTKVLYSDASKVFPKTRMTVTQGNEVLDE
                     VTVDFSQWQLPDSRVNMAEMLDGPTSRHGFVTARDGKFWRDGQEIYFFGGHENHVPGK
                     ELSDLYAETYADAGINVQRSIAFGEIVKNFETGEVDPVQLDKYLYLIAKLGERGIYFF
                     LTNAGHNGYGYPRGKTPEPGQAEAGDTYFWINPRYREVWQQAWKTILTTTNPYTGKAL
                     KDDPTLIGLELCNETGLNPRRFDYNRLDNPDQTAEWRRQFNKYLLNKYGSRAALAEAW
                     KTNPLFPHEDPAKGNILIPTNFRGSRSPYGGSGQHNQYTTGRWYRGGLPADKNPRILD
                     AIEFNNTVARKPYPFDFNNLSTPEESEKLRLAFNEFLLKKYGSREALAKAWEEDQLFP
                     WESPDVIMVKTSSARTAGYVPDPSGLARTIVIPSNYRGETEYRYEPDRRQADPRVSDA
                     IEFTYQVQKVWATDMAKFLKEEVGVKCGVGWNGDTFHMVQGPTHKANMDSPLDIAIAA
                     AYLDTDNGDQVTSRIKNLKRFNTYGRIYGRPMFSYEWSMWSNQGPFVYEYGLMAALMG
                     RTYGFDGYAHHKMSAQPYPVSDPGYSLRVHYITPLTDRPRRGSFYVSQWILQRSKIEE
                     NDQRILIGMPDDDMFIGGPERKMSNWAFENWIMYQLGIEDYSFKSVYDGPEDRIVIHD
                     GRGPYGDYRKAKHAILWCHSNSDREGKDPKAKEKWFALHGITFKPGQKYFLNDQFFAT
                     TEDMTDWNVVHRIAEKARQDEIDRNAKDDATKLSDSLRLTTSASNYWAAEPKAQPTEL
                     DRQVYRALKKWGYPLPFSEDELDKVWRSRDRSMVMDTPKQRFTADRGDMQLWFGKAGR
                     NTRISLSRLSAVTSEPQYAAALLPWDTADFATAKTLALWTLWNSEVTVKLAQANQAKV
                     YAVNWLGKRIFEVKPTQVSSNSLSFQTARHDDIFCYEIIR"
     gene            5160..7619
                     /locus_tag="PKN80_04530"
     CDS             5160..7619
                     /locus_tag="PKN80_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35313.1"
                     /translation="MAMRRQRLLLFCLMGMAGPMRIQAAPPPFPVVEAEYRVYEIGNP
                     ANGAGPLWCYGSTCLVRAGREVFASGLEIRPEQKPLNNVRWTLFRLDEKEAQRLQADS
                     EGRQREPCPIGLFKDGRLLLTSNPTLTPPGAYSGPAQPQLLIFDTKRPEAPPLLSHPQ
                     WKDNPRFTEHSYRAFAADSPNREALYLQNVGYDTAHWSFLDRDGQWSKSGSIRMPWGA
                     EFEKPQPIRICYQNLALRNRTACLLGVSDIIEWVREWREYKLILHEGNQWDYDFRRLY
                     FCRTPDITKEPFGEWIKVAECEKCGHITNLDLWLDGEGRAHILWIEQNIWDARVRNKF
                     FPGEPITHALMYGVIEGNRLVRKTCLALGGEQQASREIPGWGRFQATPDGRLFVFYYA
                     GGSDNSGKAVSENRLVEINPDGTAGQPARVNLRRPMKTFFTASERGGSAPSATLDLLG
                     EAEGISGISYARINLLGKIRVEFDWVLTPAPDGWEISLDAGSSGSTGGRIVSCSWRIG
                     EQTAAGSKVRQKIARGGPLEIELTVRDAKGNRGRFTRTFWLPPTPADLGLRQWGLLLR
                     VEAEHFAAGKGGNTQTGTGTDVSGLVLSHRGTAGDWLEWEVEVPAEDRYYLLARYLAP
                     AGALFKLAVDGQPPVEVALPAGNGERGKWDLAALTERGQPASIRLTAGRHRLRLENRT
                     DQRLDLDYLELAAAGTPFPDRKLNGWRPGEENGRRYLAALAGIIPPDRITTENGLCYN
                     YHLGPAYPGDGLESEGVAASTLRLFEDGRELGPAHAPHAEIRRQGKGRFSHWKGLLYF
                     SSSDGNDPRTNGRRYTWRLED"
BASE COUNT         1561 a         2531 c         2425 g         1135 t
ORIGIN      
        1 tgagttacct cccggtcttt ttgaaaacac ccttctacta cttcgacggc agcggcccgg
       61 tcgccacagc cctgaacgag atgctgctcc agggctggaa cggagtaatc cgggtctgtc
      121 ccgccctgcc cgaggattgg gaggcgaagt tcaccctgct gacgcccggc ggctttctga
      181 tcagcggcca ggccgcgggc ggccaggtca aatacctctc aatcctgagc cagcggggcg
      241 aaacggcggc cgtggccaac cccttcggcc ggccggccgc ggtcaacgac ggtcggcggg
      301 tcatcctccg cagcgcggcc ccggaattaa aattcccgac cgaggccggc cgggaatacc
      361 ggatttaccc ggcggatcag aatgaacccc ggccgtccat ggtaacggga gaaaccaacc
      421 aggggcccaa aacattcccg gagagttccc gccggctcgg caaatcctcc ttcgaaatcc
      481 cggaattggc cggccgcctc gaaaaaaggc cggccggccg ggatttcgaa ccggactgga
      541 cccaggcact gaaggtggcc aggacggcgg ggccggtcag gatagacggc cgcctcaccg
      601 aagcctgctg gcgcggagcg ccggccggaa cgctccgcct ggccggcggc cggcggccgg
      661 gacaacgcac cgacgtctgg ctggcggccg acgaccaggc cctctacgtc gccgccgaag
      721 cgcgcgaaga ccgaatgaag atggtccagg ccgaacgcgg cgtgcccggg gacccgctcg
      781 agccccgggt ggcgctggac gattcgctgg cgctgatcat ggaaccaatg ccgggcgccc
      841 aatggcggct gcgcgtaaat cccctgggcg tgcagaatga ctctctcttc gtcgccggag
      901 tacggccgcc cctggaagaa cgcctttccc tgatggaccc ggaatgggaa tcctccatcc
      961 ggaccaccga agacggctgg ataatggagg cccggctgga atacgcccgt ttcgcgccgt
     1021 ttccacccgg taccggccag acctggcgct tcggcgtcga acggaacgag tatccccacg
     1081 gagaacggac cgcctggccg tccgaaacca ccggcggcgc aaaggaattg gcccggctgg
     1141 agctgcccgc cagggaggcg aaggcggccg tcattgaacc ccaaccggat ctccagctgc
     1201 actggaaact ggaggccgtg gacgggccct gggtccttga cgcctccggc cacgccaggc
     1261 acggctactt ctccggggcc cgaagcggcc gggaaacgcc ggacagcgtc ctgcggttcg
     1321 caggggggac cccggatgaa aaactgaaat tcaacgccgg ctgaggtgtc gttcctttgg
     1381 gatccgggct ggccgaaagc cgggaactga ccgccgccgc ctggattaac ccggcgagtt
     1441 caaaaagcat gcgcatcctg gaaaaaccgg gaaatgaatt catctttgac atcctcgccg
     1501 gaaaactccg gctgatctcc acggccggca ccttcggcgg caccgcggcg gtgccggccg
     1561 gccggtggtc gcacgtggcc gtgacgatcg cgcccggcac gctgcgcctc ttcctgaacg
     1621 gcgaaccctg cggggaggcg cgctccggga gaccgctcac cctgagcgcg gggccgctgc
     1681 tgatcggcac caccccggac ggccggtaca gcttcaacgg aagcctggcc gacctccgtc
     1741 tctacaaccg tgccctgacg ccggctgaga tttcgaccct gcacgccggc ggccggtaaa
     1801 acgattttga cgatagcctt aaaaatatgt tttactctgg aaagtccatc cgaccgggat
     1861 ttaacggccg tctttaactt tttcaaccat ttcttaaaaa cccggagagg cgaaaatgaa
     1921 gtacggaatg ctcgttctgt cttggctgac tttagccgtt ctgaccacct ccttcagctc
     1981 cgccgaagcg gccgacgagg gaacgatcgg accgtggcgc gtcggcgaca tggcctacag
     2041 caagacgccc gccaacgacc gtccgaatgc cgagagccag cggatcgtcg aacgcaacta
     2101 taccgccctg gtctccgaga agctcctgac gggcgagaag gggaagctga ccttccgggt
     2161 tgctccccgt aatgaaaggt gggccggcat cctggtgatg gacaacgacg gcaagccgct
     2221 gccctacaat ctgaagcggg gcgcgaaggt aagcttcgcg gaggccggaa ccgggaagac
     2281 cgacccggcc gtgcgctggt accagtacga cacgaaggtg ctctacagcg acgcctcgaa
     2341 ggtctttccg aaaacccgga tgaccgttac ccagggcaac gaggtgctgg atgaggtgac
     2401 cgttgatttt tcgcagtggc agcttccgga ttcccgggta aacatggccg agatgctcga
     2461 cgggccgacc tcccggcacg gcttcgtgac ggcccgcgac gggaagttct ggcgggacgg
     2521 ccaggagatc tatttcttcg gcggccacga aaaccacgtg cccggcaagg aactctccga
     2581 cctctacgcc gagacctacg ccgacgccgg catcaacgtc cagcgcagca tcgccttcgg
     2641 cgagatcgtc aagaacttcg agaccggcga ggttgacccg gtgcaactgg acaagtacct
     2701 ctacctgatc gccaaactgg gcgagcgggg catttacttc ttcctgacca atgccggcca
     2761 taacgggtac ggatatcccc ggggaaagac gccggaaccg ggccaggccg aggcgggtga
     2821 cacctatttc tggatcaacc cgcgctaccg cgaggtctgg cagcaagcct ggaagaccat
     2881 cctgaccacg accaacccct acaccggcaa ggcgctcaag gacgacccga cgctgatcgg
     2941 cctggaactc tgcaacgaaa ccggcctgaa cccgcgccgc ttcgactaca accggctgga
     3001 caatcccgac cagacggccg aatggcgccg gcagttcaac aagtacctgc tcaacaaata
     3061 cggcagccgg gcggcgctgg cggaagcgtg gaaaaccaac ccgctctttc cgcacgagga
     3121 cccggccaaa ggcaacatcc tgatcccgac caacttccgc ggttcgcgca gcccttacgg
     3181 cggcagcgga cagcacaacc agtacacaac cggccgctgg taccggggcg gcctgccggc
     3241 ggataagaac ccgcgcatcc tggatgccat cgagttcaac aatacggtgg ccaggaagcc
     3301 ctacccgttc gatttcaaca acctgagcac cccggaggag agcgagaagc tccgcctggc
     3361 cttcaacgaa ttcctgctca agaagtacgg cagccgcgag gcgctggcca aggcctggga
     3421 ggaggaccag cttttcccgt gggagtcgcc ggacgtgatc atggtcaaaa ccagttcggc
     3481 ccgaacggcc ggatacgtgc ccgacccgag cggtctggcc cggacgatcg tcattcccag
     3541 caactaccgt ggggaaaccg agtaccggta cgagccggac cggcgccagg ccgacccgcg
     3601 ggtcagcgac gccatcgagt tcacctacca ggtgcagaag gtctgggcga ccgacatggc
     3661 gaaattcctc aaggaggagg tcggggtgaa gtgcggggtg ggctggaacg gcgacacctt
     3721 ccacatggtc caggggccca ctcacaaggc gaacatggac tcgccgctgg acatcgccat
     3781 cgccgccgcc tacctggaca ccgacaacgg cgaccaggtc accagccgga tcaagaacct
     3841 caagcgcttc aacacctacg gccggatcta cggtcggccc atgttcagtt acgagtggag
     3901 catgtggtcg aaccaggggc cctttgttta cgagtacggc ctgatggcgg ccctgatggg
     3961 gcggacttac ggcttcgacg gctacgccca ccacaagatg agcgcccagc cctacccggt
     4021 gagcgatccg ggctactccc tgcgggttca ttacatcacc cccctgacgg accggccgcg
     4081 gcgcggctcc ttctacgtca gccagtggat actgcagcgc tctaagatcg aggagaacga
     4141 ccagcggatc ctgatcggca tgcccgacga cgacatgttc atcggcggcc cggaaagaaa
     4201 gatgagcaac tgggccttcg agaactggat catgtaccag ctgggcatcg aggattactc
     4261 attcaagagc gtctatgacg ggccggagga ccggatcgtc atccatgacg gccgcggacc
     4321 ctatggcgac taccggaagg cgaagcacgc catcctctgg tgccacagca acagcgaccg
     4381 cgagggcaag gacccgaagg cgaaggaaaa gtggttcgcc ttgcacggca tcaccttcaa
     4441 gccggggcag aagtacttcc tgaacgacca gttcttcgcc accaccgagg acatgaccga
     4501 ctggaacgtg gtgcaccgga tcgccgagaa ggcgcgccag gacgagattg accggaacgc
     4561 caaggacgac gcgaccaagc tcagcgattc gctccgcctg acgacctcgg ccagcaatta
     4621 ctgggcggcc gagccgaagg cccagccgac cgaactggac cggcaggtgt accgggccct
     4681 gaagaagtgg ggttatcccc tgcccttctc agaggatgag ctggacaagg tctggcgcag
     4741 ccgggaccgg agcatggtca tggacacccc gaagcagcgc ttcacggccg accgcggcga
     4801 catgcagctc tggttcggca aggccggccg gaacacccgg atctccctca gccgcctctc
     4861 ggccgtcacc agcgagccgc aatacgcggc ggccctgctg ccctgggaca cggccgactt
     4921 cgccacggcc aagacgctgg ccctctggac gctgtggaac agcgaggtca cggtgaaact
     4981 ggcccaggcc aaccaggcga aggtttacgc ggtcaactgg ctcggcaagc ggatcttcga
     5041 ggtgaagccg acccaggtga gttccaacag cctcagtttc cagacggcgc gccacgacga
     5101 tatcttctgc tacgagatca tccggtaaaa ttcaccggcg cgagggataa agagacgggg
     5161 tggcgatgcg aaggcagcgg cttcttctat tctgcctgat gggaatggcc gggccgatga
     5221 gaattcaagc cgcaccgccg cccttcccgg tcgtggaggc ggagtaccgg gtctatgaaa
     5281 tcggcaaccc ggccaacggc gccggccccc tctggtgcta cggctcgacc tgcctggtcc
     5341 gagccggccg ggaggtcttc gccagcggcc tggagatccg gccggagcag aaacccctca
     5401 acaacgtgcg ctggacgctc ttccggctgg atgaaaagga agcacagcgg ctccaggccg
     5461 attccgaagg acgccagcgc gaaccctgcc cgatcggact cttcaaagac ggccgcctcc
     5521 tactgaccag caacccgacc ctgaccccgc cgggcgccta ctccggcccg gcccaacccc
     5581 agctgctgat cttcgacacc aagcgccccg aggcgccgcc gcttctctcc cacccgcaat
     5641 ggaaggacaa cccgaggttc accgagcact cctaccgcgc cttcgccgcg gacagcccga
     5701 accgggaggc gctctacctg cagaacgtcg gttacgacac cgcccactgg agtttcctcg
     5761 accgcgacgg ccaatggtca aagtccggct cgatcaggat gccctgggga gccgagttcg
     5821 aaaagcccca gccaatccgc atctgctacc agaacctggc cctgcgcaac cggacggcct
     5881 gcctgctggg cgttagcgac atcatcgaat gggtgcggga gtggcgggag tacaagttaa
     5941 tcctgcacga aggcaatcaa tgggattacg acttccggcg cctctacttc tgccggacgc
     6001 ccgacatcac gaaagaaccg ttcggtgaat ggatcaaggt cgccgaatgc gaaaagtgcg
     6061 gacatatcac caacctcgac ctctggctgg acggagaggg gcgcgcccac atcctctgga
     6121 tcgagcagaa catctgggat gcccgggtcc ggaacaaatt ctttccgggc gagccgatca
     6181 cccacgcgct gatgtacggg gtgatcgaag gcaaccggct ggtgcgaaaa acctgcctgg
     6241 ccctgggggg tgagcagcag gcgagccgcg agatcccggg ctgggggcgc ttccaggcga
     6301 cgccggacgg ccgcctcttc gtcttctatt acgccggcgg ttcggacaat tccgggaaag
     6361 cggtctccga gaaccgcctg gtcgagataa atccggacgg gacggccggc caaccggcgc
     6421 gggtaaacct gcgccggccg atgaagacct tcttcacggc cagcgaacgc ggcggctcgg
     6481 ctccgtcggc caccctggac ctgctcggag aggccgaggg gatcagcggc atcagttacg
     6541 cccggatcaa cctgctgggc aagatccggg tcgaattcga ctgggtgttg acgccggcgc
     6601 cggacggctg ggaaatcagc ctcgacgccg gcagctcggg gtcaaccgga ggaagaatcg
     6661 tctcctgctc ctggcggatc ggagagcaaa cggcggccgg ttcgaaggtc cggcagaaaa
     6721 tcgcgcgcgg cggtccgctg gagatcgaac tgaccgtgcg ggacgcgaag ggcaaccgcg
     6781 gccgtttcac ccggaccttc tggctcccgc ccacgccggc cgacctcggc ctcaggcaat
     6841 ggggactgct cctgcgggtg gaggccgaac atttcgccgc cgggaagggc ggaaacaccc
     6901 agaccggcac cggaaccgac gtttcgggcc tggtcctttc acaccggggc acggcgggcg
     6961 actggctcga atgggaagtc gaggtgccgg cggaagaccg ctactatctc ctggcccgtt
     7021 acctggcgcc ggctggagcg ttgtttaagc tggccgttga cggccaaccg ccggttgagg
     7081 ttgccctgcc ggccggaaac ggggaaagag ggaagtggga cctggccgcc ctgacggaac
     7141 ggggacagcc ggcctcgatc aggctgaccg ccggacgcca ccggctgagg ctggagaacc
     7201 ggacggacca gcgccttgac cttgattacc tcgaactggc ggccgccgga acgccctttc
     7261 cggaccgaaa attaaatggc tggcggcccg gcgaagaaaa tggtcgccgg tacctggccg
     7321 ctctggccgg cataattcca ccagaccgga tcaccaccga aaacggcctc tgctacaatt
     7381 accacctggg cccggcctat cccggcgacg gccttgaaag cgagggtgtg gcggcctcaa
     7441 cactgcgcct cttcgaagac ggccgggaac tgggtccggc ccacgcgcct cacgccgaaa
     7501 tccggcggca gggcaagggc cgcttctccc actggaaagg gctgctttac ttctcctcga
     7561 gcgacgggaa cgatccacgc accaacggcc gccgttatac ctggcgcctc gaggattgac
     7621 gcggcgggat aaaaccgtcc gaaaagagaa tg
//
LOCUS       DAOQYN010000084         7590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_10079_length_7590_cov_4.048706, whole genome shotgun sequence.
ACCESSION   DAOQYN010000084 DAOQYN010000000
VERSION     DAOQYN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10079_length_7590_cov_4.048706"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..2297)
                     /gene="fusA"
                     /locus_tag="PKN80_04535"
     CDS             complement(201..2297)
                     /gene="fusA"
                     /locus_tag="PKN80_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNQ35314.1"
                     /translation="MEDAPKNGEAQVVEQHIQLARLRNIGIIAHIDAGKTTTTERMLY
                     YSGKVYRLGDVDEGTTVTDWMPQERERGITITSAAITFDWLDHQITIIDTPGHVDFTM
                     EVERSLRVLDGAVGIFCAVGGVEPQSETVWHQANRYRVPRIAYINKMDRVGADFFQTV
                     AMIRDRLGADPYPIQLPIGQGEKFSGLIDLLEMEAITYSDEAGTQIVRGPVPEAMKDE
                     AEQRRRELLERLAESDEELMSLYLEESPISVEMIRKVLRQETLSGRILPVLCGSSFRN
                     RGVQFLLDAICHYLPSPLDLPPVKGLDPDRDREEQRVPSPKENFAGLVFKITTDPYVG
                     RISYLRVYSGTLRAGAVTYNSLREKRERINRILRMQGNRSSDLEYTEAGEIVALVGLK
                     NTRSGDTLCSEKHPIVLEKMTIPEPVIAVAVEPKSRADSDKIGIALAKLSEEDPTFKV
                     RVDGETGQTIISGMGELHLDIIRDRLVREFKVEARVSQPQVAYRETITCAAEAQGRFV
                     RQTGGKGQYGDVWLRIEPLARGSGNQFEEKIKGDDVPREFFRAIEQGVMESAETGVLE
                     GYPVIDFKATLFDGSYHPVDSSEIAFKIAASMGFKEAVRKAKPVLLEPIMKLEIMTPE
                     EFLGDVLGDITSRKGRVEEIRERGVLRAIRACAPLANLFGYATSLRSLTQGRASYVME
                     PSHYEPLPEGRRPEAR"
     gene            complement(2317..3507)
                     /locus_tag="PKN80_04540"
     CDS             complement(2317..3507)
                     /locus_tag="PKN80_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNQ35315.1"
                     /translation="MKITVNLEKCTGCRACVKACPFDAISMVEKKAVIDLDKCTYCGS
                     CVEACRFEAIAIEREPAAVSTENLESYRGVWVFAEQRSGHLARVAFELLGEGRRLAGD
                     LGQPLCAAYIGPENGSVESLLKRGADRVYAVTDDSLLHFQDEPYAAILTYLVRTYRPA
                     ILLAGATYIGRSFIPRVAARLRTGLTADCTGLAIDPETKLLAQTRPTFGGNLMATIFC
                     EKNRPQMATVRPRTFDEAPVGAGGGEAVRVPFSEIAAGNRVTLDRIIPGSEEIDLTDA
                     EIIVSGGRGLGKPEGFALIKGLADALGGVVGASRAAVDAGWISYAHQVGQTGKTVKPK
                     LYFACGISGAIQHLAGMGTSDVIVALNKDPEAPIFKVANYGLVGDLYEIIPALVKRLK
                     QGGA"
     gene            complement(3541..4329)
                     /locus_tag="PKN80_04545"
     CDS             complement(3541..4329)
                     /locus_tag="PKN80_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNQ35316.1"
                     /translation="MEKIIVFIKQVPDTMEVRIDPETNRIIREGVPSIINPYDRYAME
                     AALRLKESFGSRAVVACMGPPQAEAALREAMSLGFDQAILLSSRDFAGSDTLATSYTL
                     AALVRKLAPVDVVVCGREAMDGSTGQVGPEMAEHLGWPFLGFVSKVEELKEGRLRVNR
                     MMEDHYEEIEGPLPLVMTVVKEIAEPRLPSLRGLLQAKKAQIQVWGREELEMEPARTG
                     SAGSPTWVKRVWTPEHHTQGMKLTGEPEELVDRLYKELKGQNLL"
     gene            complement(4402..5835)
                     /locus_tag="PKN80_04550"
     CDS             complement(4402..5835)
                     /locus_tag="PKN80_04550"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNQ35317.1"
                     /translation="MAYQLERCDDYRYRLAPTGAMRVPGLVYASPVLLPKILADRSVE
                     QVANVACLPGILRYSLAMPDIHWGYGAPIGGVAAFDLREGVAVPGITGYDINCGVRLL
                     GAKLPAEAVTGRVQEVVEALYRAVPCGVGKAGRFRLKKGQMARVLAEGAAWAVREGYG
                     STADLEHMEDGGCLEGADPEQVSPRALERGSSQLATLGSGNHFLELQQVEQVFEPAAA
                     DAFGLFPGQLTVMIHTGSRGLGYQVADDYLASFGRAASRYGIRLPDRQLACAPVESPE
                     GRDYLGAMAAAANFAFANRQLIAHDAGQALARSLERSDLELVTVYDIAHNIARFESHR
                     VEGREVRVLVHRKGATRAFPAGRSEVPADYRAVGQPVLIPGTMGTASYVLVGTSAALE
                     EAWGSSCHGAGRSLSRSSAVRSMKGRSAVEELAARGVAVRAPSLRGLAEEMPEAYKDV
                     EQVIEAVEGGGLARRVARLVPFAVIKG"
     gene            complement(6307..>7590)
                     /locus_tag="PKN80_04555"
     CDS             complement(6307..>7590)
                     /locus_tag="PKN80_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35318.1"
                     /translation="CTFDIKGTTWVALHAPAPALLKRISLAVPAADLTILDMHLAPRP
                     AAAVFATRSGETQFLMRRGETLFVGSRNPAFLEAARNLKATDEIDCRYAVLAREGKTV
                     CAGFLKNNGAGKVTGRLDLYRNMLYRQGRESFPFALGPGEERIFEVAQEAGAFDGRPL
                     TYYPAAAELTLDGFITAQPGEVEEALKATAEGQYMRGDNYFWLHYALPLSKEAAGLEV
                     KRLEEWEETQSPASVYINWGLNGSYQRTQVRWGAENTDPNMRLDGSMDCDFMSRHYVR
                     YDAQALYLGAIIEDQPSFAGAADPAAWGESVEYRLQPGLDGQLGLHRAPALRAIRVVQ
                     LAPDRLLATLAGPDGKTVALEGVCASRGDRREFLVRVPLELLGGGLKAGATLGLGITC
                     FDSDDPSGKAEVEYDWSGAEFAPDDAFGFGQLILK"
BASE COUNT         1320 a         2639 c         2278 g         1353 t
ORIGIN      
        1 ttgacgtgcg gcttgtcccc ccgctcaaac ttttccttcg ccatgccgtt cctcccggga
       61 aaacacccga tttgctgatc cgtttatttt ttaaattact gcaaattttc gagaaaagaa
      121 ttgtatcaca atccgtttcg gtcggtcaaa tctgccggcc ccttccaacc gggcgccgcg
      181 gccgcccggg ccagcccggt tcagcgcgcc tcggggcggc gtccctccgg cagcggttcg
      241 tagtggctcg gttccatcac gtagctggcc cggccctggg taagggaacg cagggaggtc
      301 gcgtaaccaa agaggttggc cagcggcgcg caagcccgga tcgcccgcag cacgccccgc
      361 tcccggatct cctccacccg ccccttccgg ctggtgatgt cccccagcac gtcgcccagg
      421 aactcctccg gggtcatgat ctccagtttc atgatcggct ccagcaggac cggtttcgcc
      481 ttccgaaccg cctccttgaa gcccatcgag gcggcaatct tgaaggcgat ctcggaggaa
      541 tcgaccgggt ggtaggaacc gtcgaagagg gtggccttga aatcgatcac cgggtaaccc
      601 tccagcacgc cggtctcggc cgactccatc acgccctgtt cgatggcccg gaagaactcc
      661 ctgggcacgt cgtcgccctt gatcttctcc tcgaactggt tgccggagcc cctcgccagg
      721 ggctcgatcc ggagccagac gtcaccgtac tggcccttgc cgccggtctg gcgcacgaac
      781 ctgccctggg cctcggcggc acaggtgatc gtctcccggt aggccacctg gggctggctc
      841 acccgcgcct caaccttgaa ctcccggacc aaccggtcgc ggatgatgtc caggtgcaac
      901 tcacccatcc cggagatgat cgtctggccg gtctcgccat ccacccggac cttgaaggtc
      961 ggatcctcct cggaaagctt ggcgagggcg atgccgatct tgtcgctgtc ggcccggctc
     1021 ttgggttcga ccgccaccgc gatcaccggt tcggggatgg tcatcttctc cagcacgatc
     1081 gggtgcttct cgctgcagag ggtatcgccg gaacgggtat tcttcaggcc caccagggcc
     1141 acgatctcgc cggcctcggt atattccagg tccgaactcc ggttgccctg catgcgcagg
     1201 atccggttga tgcgctcccg tttttcgcgc aggctgttgt aggtgacggc cccggcccgc
     1261 aacgtgccgg agtaaacccg caggtaggat atccgcccca cataggggtc ggtggtaatc
     1321 ttgaagacca ggccggcgaa attctctttc ggggagggaa cccgctgctc ttcgcggtcc
     1381 cggtccgggt ccagaccctt gaccggcggc aggtccagcg gcgaaggcag gtaatggcag
     1441 atggcgtcca gcaggaactg cactccgcgg ttgcggaagg aggaaccgca gagcaccggc
     1501 aggatccggc cggaaagcgt ctcctggcgg agcaccttgc gaatcatctc gaccgaaatc
     1561 ggagactcct ccaggtagag ggacatcagc tcctcgtcgc tttcggccag gcgctccagg
     1621 agttcccggc gccgctgttc ggcctcgtcc ttcatcgcct ccggcaccgg cccccggacg
     1681 atctgggtgc ccgcctcgtc agagtaggta atggcctcca tctccagcag gtcgatcaga
     1741 ccggaaaatt tttcgccctg gccgatcggc agctggatcg gatagggatc ggcgcccagg
     1801 cggtcgcgga tcatggcgac cgtctggaaa aaatcggcgc ccacccggtc catcttgttt
     1861 atgtaggcga tgcgcggcac ccggtaacgg ttggcctggt gccagacggt ctccgactgg
     1921 ggttccaccc cgcccacggc gcagaagatg ccgaccgcgc cgtccaggac ccgcagggaa
     1981 cgctccacct ccatggtgaa atcaacgtgt cccggggtgt cgatgatggt gatctggtgg
     2041 tcgagccagt cgaaggtaat ggcggccgaa gtgatggtga taccccgttc ccgctcctgg
     2101 ggcatccagt cggtcacggt cgtaccctcg tccacgtcgc ccaggcggta aaccttgcca
     2161 ctgtaataga gcatccgctc ggtggtggtg gtcttcccgg cgtcgatgtg ggcgatgatg
     2221 ccgatattgc gcaggcgggc cagctggatg tgctgctcaa ctacctgggc ttctccgttc
     2281 tttggcgcat cttccatggt tttcaaggtc ccgggttcag gcgccgccct gtttcaggcg
     2341 tttgaccaac gccgggatga tttcgtagag gtcccctacc aggccgtaat tggccacctt
     2401 gaagatgggc gcctcggggt ccttgttgag ggccacgatg acgtcggagg ttcccatgcc
     2461 ggccaggtgc tggatggcac cggagatgcc gcaggcaaaa tagagcttcg gcttgaccgt
     2521 cttgccggtc tgcccgacct ggtgggcgta ggaaatccag ccggcgtcaa cggccgcccg
     2581 gctggccccc accacgccgc ccagcgcgtc ggccagcccc ttgataagag cgaatccttc
     2641 cggcttgccc aggccgcggc cgccggagac gatgatttcg gcgtcggtca ggtcgatctc
     2701 ctcggagccg ggtatgatcc ggtcgagggt gacccggttg ccggccgcga tctccgaaaa
     2761 gggcacccgg accgcctcgc cgccgccggc gccgaccggc gcctcgtcga aggtgcgcgg
     2821 ccgcacggtg gccatctggg gccggttctt ctcgcagaag atggtggcca tcaggttgcc
     2881 gccgaaggtc ggccgggtct gggccagcag cttcgtctcc gggtcgatgg ccaggccggt
     2941 gcagtcggcg gttaggccgg tccgcagacg ggccgccacc cggggaatga aggaacggcc
     3001 gatgtaggtc gccccggcca gcaggatcgc cggccggtag gtccgcacca ggtaagtcag
     3061 aatagccgcg tagggctcat cctggaaatg gagcagggaa tcgtcggtca ccgcgtaaac
     3121 ccggtcggcg ccgcgcttca gcaaggactc gacgctgccg ttctcgggac cgatgtaggc
     3181 ggcgcacaga ggctggccga ggtcgccggc cagccgccgc ccctcgccca gcagctcaaa
     3241 ggccacccgg gccaggtggc cggagcgctg ctcggcaaag acccagacgc cgcggtaact
     3301 ctccaggttc tcggtcgaca ccgccgccgg ctcccgctcg atggcaatcg cctcgaaccg
     3361 gcaggcctcg acgcaggaac cgcagtaagt acacttgtcc aggtcgataa ccgccttctt
     3421 ctcgaccatc gagattgcgt cgaagggaca ggctttgaca caggcccggc aaccggtgca
     3481 tttttccagg tttacggtta tcttcatggg attttcagat caaaattggg aaaagggggg
     3541 ttacaggagg ttctggccct tcaattcctt gtagagacgg tcaaccagct cctcgggctc
     3601 gccggtgagt ttcattccct gggtgtggtg ttccggggtc cagacgcgct tgacccaggt
     3661 gggcgaaccg gccgacccgg tccgggccgg ctccatctcc agttcctccc ggccccagac
     3721 ctggatctgc gccttcttgg cctggagcag gccgcggagc gagggcaggc gcggctcggc
     3781 aatctccttg accaccgtca tcaccagcgg cagcggcccc tcgatctcct cgtaatgatc
     3841 ctccatcatc cggttaaccc gcaaccggcc ctccttgagc tcctcgacct tgctcacgaa
     3901 gcccaggaaa ggccagccga ggtgctcggc catctccggc ccgacctggc cggtggaacc
     3961 gtccatcgct tcccggccgc agaccaccac gtccaccggc gccagcttcc ggaccagcgc
     4021 ggccagtgtg taggaggtgg ccagggtgtc gctgccggcg aaatcccggc tcgaaagcag
     4081 gatcgcctgg tcgaagccca ggctcatcgc ctcgcgcagg gcggcctcgg cctgaggcgg
     4141 ccccatgcag gcgaccaccg cccggctccc gaagctctcc ttgagcctca gggccgcctc
     4201 catggcgtac cggtcgtagg ggttgatgat gctgggcacc ccttcgcgga tgatccggtt
     4261 ggtctccgga tcgatcctga cctccatggt gtccggcacc tgcttgataa aaacgataat
     4321 cttttccata agccttgtaa gtaattctct tccgggactt cccgcccgtt aaaattaaac
     4381 acaagattat aattatacca tctacccctt aatcaccgca aagggaacca gccgggccac
     4441 ccgacgagcc aggccgccgc cctcaaccgc ctcgatcacc tgctccacgt ccttgtaggc
     4501 ctcaggcatc tcctcggcca ggccgcgcag gctgggcgcc cggaccgcca cgccccgagc
     4561 cgccagttcc tccaccgccg aacgtccctt catgctgcgc accgccgaac ttcgggacag
     4621 gctgcggccg gcgccgtggc aactcgaacc ccaggcctcc tccagggccg ccgacgtgcc
     4681 aacaagcaca taagaagcag tccccatcgt ccccggaata agcaccggct gtccaactgc
     4741 ccggtagtcg gccggcacct cgctccggcc ggccggaaag gcccgggtcg cccctttgcg
     4801 gtgtaccagt acccgaacct cccggccctc aacccgatga ctctcaaagc gggcgatatt
     4861 atgagcaata tcatagaccg taaccaattc caaatcgctg cgttccaggc ttcgggccag
     4921 cgcctggccg gcgtcatggg cgatcaactg ccggttggca aaggcaaagt tggcagccgc
     4981 ggccatagcg cccaggtaat cccgcccctc aggcgactcc accggcgcgc aggccagttg
     5041 acggtccggc aggcgtattc cgtagcggct ggccgcccgg ccgaaggagg ccaggtaatc
     5101 gtcggccacc tggtaaccca gcccccggct cccggtgtga atcatcaccg tcaactggcc
     5161 gggaaagaga ccaaaagcat cagcggccgc cggctcaaag acctgctcca cttgctgcaa
     5221 ctcgagaaaa tggttgccgc tgcccagggt ggccaactgg ctggagccgc gctccagcgc
     5281 ccgcggactg acctgctccg ggtcagcccc ctccaggcag ccgccatcct ccatatgctc
     5341 caggtccgcc gtcgagccgt acccctcacg caccgcccag gccgcccctt ccgccagtac
     5401 ccgggccatt tgccccttct taagccgaaa ccgacctgcc ttacccaccc cgcagggaac
     5461 agcccgataa agcgcctcaa caacctcttg cacccggccg gtaaccgctt ccgccggcaa
     5521 cttcgcgccc agcaggcgca ccccgcagtt gatatcatac ccggtaatac ccggcaccgc
     5581 cacgccctcc cgaaggtcaa aggccgccac cccgccgatc ggcgcgccgt acccccagtg
     5641 aatatccggc atcgccagcg aataacgcag gatacccggc aggcaggcaa cattagccac
     5701 ctgctcaacc gagcgatccg ccaggatttt aggcagcaac accggcgagg catagaccag
     5761 ccccggcacc cgcatcgcgc ccgtgggcgc cagccggtac cggtaatcgt cgcatcgttc
     5821 cagttggtat gccatcttgt tcccccgtcc ccacggactt gtccgccgta gcttgttgtt
     5881 tagcgtagga ggatagtaag tgggaatcca gcagttgcct tttaaaatat ttgcaagttt
     5941 atcaggcagc ctggataccc gctaaccacc cgcgggtatg acatgcaaaa cattcccgcc
     6001 atgccttttt ttgtcattcc ctcgtgcctg tccccgaaag tagtagtcgg ggatccaaaa
     6061 ccttaaatgc acgtcattcc ctcgtgtggt aagagggaat ccaatcctta aagctcttcg
     6121 ggtttttcaa gtagcctgga tccccgactc aaacattcgg ggatgacgtg caaaaaaaca
     6181 tttttagtgt gtcattcccg tatgtggtaa acgggaatcc agcatttaat gccgttgtag
     6241 ttttccaacc agcctggatc cccgactaaa gcattcgggg atgacaggct tgttataatc
     6301 ctccccctac ttcaaaatca gctggccgaa gccgaaggca tcatcggggg cgaattcggc
     6361 gccggaccag tcatactcga cctcggcctt cccggacgga tcgtcgctgt cgaagcaggt
     6421 aatgcccagt ccgagcgtgg cgcccgcctt caggccgccg cccagcaact ccagcggcac
     6481 gcggaccagg aactcccgcc ggtcgccgcg gctggcgcag accccctcca gcgcaaccgt
     6541 cttcccgtcc ggcccggcca gggtggccag cagccggtcc ggggccagtt gcaccacccg
     6601 gatcgcgcga agtgccggcg cccggtgcaa gccaagctgg ccgtccagac cgggctgcag
     6661 ccggtactcc acgctctccc cccaggccgc cggatcggcc gcgcccgcga aggagggctg
     6721 gtcctcgatg atcgccccga ggtagagcgc ctgcgcgtcg taccggacat agtggcgcga
     6781 catgaaatcg cagtccatcg agccgtcgag gcgcatgttg ggatcggtat tctcggcccc
     6841 ccaccggacc tgcgtccgct ggtaggagcc gttgagaccc cagttgatat agaccgaggc
     6901 cggcgactgc gtctcctccc actcctcgag ccgcttcacc tcgaggccgg ccgcctcctt
     6961 cgaaaggggc agcgcgtagt gcagccagaa ataattgtcg ccccgcatgt actgcccttc
     7021 ggccgtggcc ttcagggcct cctcgacctc gcccggctgg gcggtgatga agccgtccag
     7081 ggtaagctcc gcggcggccg ggtaataggt gagcggccga ccgtcaaagg cccccgcctc
     7141 ctgggccacc tcgaagatcc gctcctcgcc cggaccgagc gcgaacggaa aactctcccg
     7201 cccctgccgg tagagcatgt tccggtacag gtccagccgg ccggtcacct ttccggcgcc
     7261 gttgttcttc aggaagccgg cgcagacggt cttgccctcc cgggccagta ccgcgtatcg
     7321 gcagtcgatc tcgtcggtcg ccttcaggtt ccgcgccgcc tccaggaagg ccgggttccg
     7381 gctgccgacg aagagcgttt ccccgcgccg catgaggaat tgcgtctccc cgctccgcgt
     7441 cgcgaagacg gccgccgccg gccgcggggc caggtgcatg tcgagaatgg tcaggtccgc
     7501 cgccgggacc gccagcgata tccgcttgag cagcgccggc gccggcgcgt ggagcgccac
     7561 ccaggtcgtc cccttgatgt caaaggtgca
//
LOCUS       DAOQYN010000085         7465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_10292_length_7465_cov_5.178003, whole genome shotgun sequence.
ACCESSION   DAOQYN010000085 DAOQYN010000000
VERSION     DAOQYN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10292_length_7465_cov_5.178003"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PKN80_04560"
     CDS             complement(<1..357)
                     /locus_tag="PKN80_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HNQ35319.1"
                     /translation="MKRKKAILLLDDGAWWEGAAAGAEGEVFGELVFYTGVVGYQEIL
                     TDPALAGKLAVMTYPHIGNYGIVPADDLSGRVWPGALLVKELSCTYSNWQARSGLPEF
                     ARANGLFILDGIDTQSV"
     gene            complement(354..1673)
                     /locus_tag="PKN80_04565"
     CDS             complement(354..1673)
                     /locus_tag="PKN80_04565"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF024927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNQ35320.1"
                     /translation="MFSLNENCGLFGIYSDQPCVNELYYGIFKLQHRGQRYCGLATWR
                     RGLRIFTHKGYVRHTFTPAELDELRARSGIGHVSLKERQPMLLDSRLGPFAIAFTGNI
                     INAQRLTDDLKKNGHTFSTQHHVELLGKLIAQGSDFADGLAALARQVRGSYCVLLLTR
                     EGIYAARDPHGFKPLVLGRKNGALAVASESRPFNTLDFELLRDVRPGEILFLGRNGFE
                     TLKVIESEHISHCAFEWGYIASMDSIIDGVPVVRARKNLGASLARHDRVQADKVAAIP
                     FSGIGYALGYHQESGLPYDEVFLIDRFASRSYLPLIKAERDEEARIKISVIAENVKGK
                     SIVLCDDSIVRGTQIRNKVFELKSFGAREVHVRVGCPPLMAPCIYDISTRSYRELAAR
                     KYALEEIRRRVGADSLSYNSLEDLVQAIGLPKHRLCLACWAGKGSKR"
     gene            complement(1773..2858)
                     /locus_tag="PKN80_04570"
     CDS             complement(1773..2858)
                     /locus_tag="PKN80_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNQ35321.1"
                     /translation="MKTRIRFLEFLLLAAALLAGRPAGAATLEEKIGQLIMVGFEGRD
                     FSELDTRLRQQLSRGEIGGLLFLPRNLGEPDHLRRLVAEIKAQPVPRPLLLAIDQEGG
                     PVGRLNAKRGFRDFPSARRAARELTPAAAGGVYLEMARLLKEIGFNLNLAPVVDLNIN
                     PDSPAIGRKERAFAADPATVVRYASAFIRAHRRAGLLTALKHYPGHGSAAEDSHLGLT
                     DVTATWTAAELEPYRRLIRAGLADAVLTAHVIQAGVDPDWPASLSTRHLKENLRGKLG
                     FRGVIITDDLQMGAVSRLYSFEEAVVRALDAGSDILLFANYFTPDREAPARAGRAILA
                     AVRAGRLSPERIDQSYQRVLRFKSRLR"
     gene            3110..6529
                     /locus_tag="PKN80_04575"
     CDS             3110..6529
                     /locus_tag="PKN80_04575"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35322.1"
                     /translation="MLIGAFDNAAFLGDPAGFAVVTTPLPKAYSITNLPNGSYYLGAF
                     LDLNGDNQRQAEEPSGYYAGNPVAVTALAPNPTGRNLTLTVPALQEEYRFHSLLFGPD
                     DENGFQRGRVRLYNNGQAAVEFEGESGLTASWSRDGHGFTTFLSESDGEVDTQLFYGA
                     GDFMITASGGIAQDGPDPASRLYEAQLMVAVRKQAVTYSAVDLAGEYNLVEMECGGAH
                     GNSWVRGVVSVDGDGNLTFDLVDSDGQAHQGGFGPLAISADGIITADGQPDFKGVMSP
                     EKRAVLATMTSRPGVYSLQMLARRSNGLNGASLPGGYTFFEATVSDSHSQVEARLGTL
                     EVEADLDYRVLEWCRMKYDSLLYHQGRFAFVPEQPGVFAPVSNPYLAAFGGLEVGSGV
                     KLLASSRLGPAGGYAVAIGLSGPDYAADRTAIAALTTNFVAQYNSNPANIGPFFDNDF
                     YFRGMNKAAAVADMLPEAGEDPYVVVSGLENIILRDRALTFFHLQMRDGGAAGPVIEE
                     GDEAYDYLNYLRHNGSAWLFFGNQSRSGPPQAAAINWSRSETSNGGRVQFEVESDGFV
                     INDCQVAGPGVTDPVQFNYWSDQEEGYSQASGQLEDFTPPLGAVYTFRVAYDNRVELI
                     QDSVESLFTTAPRVTAPADGVILADTSGNRRPDFAWTMETDGEPQATSYKIWLERTGQ
                     DWQRFFDFYDLPLSRFLVSGSNYRAAYADYQEELPDGVSELSPGNYSFRVNLYDRFNN
                     GAEGISDFGIGQGRLSIRSQYWNCQPAPGDYFYNYNTPVNARADAVVDLGGYTRAACT
                     GWTGSGAVAAQGFENEVNFNLTANSTLEWHWSVQHWLSKSVNGIGCDVLGADTWIEAG
                     SRATVTAVAGAGWFFDHWVIDGVDRTDLDTPVLSLTVDNPVQVHAIFEELWPLDIEIS
                     PAGFGSVSLEPAPAAAPNFYPAGQLVNLTARPNGGYAFQGWNGVDTVSGSTAQVTVGW
                     GGRSVTAFFNNGPWLTVSDTALVFTADPQALSLTLTNTGGENLGWEAAEAIPWLGLSK
                     TAGALAKDGGSEIITLTRTGGAAGSRGVVTFQQPAGSFQSPIEVTVVIPAPGDLNFDL
                     TVDLADAELALRILVDPESYSEAQRQAADLDGSGAVDIEDVVAVYRLLLGI"
     gene            complement(6548..>7465)
                     /locus_tag="PKN80_04580"
     CDS             complement(6548..>7465)
                     /locus_tag="PKN80_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNQ35323.1"
                     /translation="PVYPASYNLPNIIAVAASTAADSLASFSNYGAASVHLAAPGVNI
                     LSTVPMAAAVTAGGVTSEAEGLEYSGYTDGITRPIYYCGIGNPGDFPPQVSGNLALIQ
                     RGTLYFSEKTANAQAAGAAAVVIYNNIPTGDPDGGLILGTLGSPGDWVPAVFITREDG
                     ERIRALGNPLTMVTYSKSSAYSSRDGTSMATPFVAGAVALVAARYPDEPAGRWRERII
                     SGVDKPAGLSGKLISGGRLNLRQALDLFLKGDVNRDGKLDLADALAVLRMSLGLPAEG
                     DRTAADLDEDGQVTARDLAALVGRIGGGY"
BASE COUNT         1227 a         2306 c         2622 g         1310 t
ORIGIN      
        1 gaccgactgg gtgtcaattc catccaggat gaagaggccg ttggcgcggg cgaactccgg
       61 caggccgctt cgggcctgcc agttgctgta ggtgcagctc agttccttga ccaggagcgc
      121 cccgggccag acccggccgg agaggtcgtc ggcgggcacg atgccgtagt tgccgatgtg
      181 gggataggtc atcaccgcca gcttgcccgc cagggccggg tcggtcaaga tctcctggta
      241 gccgaccacg ccggtgtaga agaccagttc gccgaagacc tctccctcgg cgccggccgc
      301 ggcaccctcc caccaggcgc cgtcgtccag gagcaggatg gctttcttcc tcttcatcgt
      361 ttgcttccct ttccggccca gcaggccagg cagagccggt gcttcggcag cccgatggcc
      421 tggaccaggt cctccagcga attgtagctg agactgtcgg cgccgacccg gcggcggatt
      481 tcctccagcg catacttgcg ggcggccagt tcccggtaag agcgggtcga gatatcgtag
      541 atgcagggtg ccatcagggg tgggcagccg acccggacgt gcacctcccg ggcgccgaag
      601 gacttgagtt cgaagacctt gttgcggatc tgggtcccgc gcacgatgga atcgtcgcag
      661 aggacgatgc tcttgccctt gacgttctcg gcgatcaccg agatcttgat gcgggcctcc
      721 tcgtcccgct cggccttgat cagcggcagg tagctgcggc tggcgaagcg gtcgatcagg
      781 aagacctcgt cgtaagggag gcccgattcc tggtggtaac cgagggcgta gccgatgccc
      841 gagaagggga tggcggccac cttgtcggcc tggacccggt cgtggcgggc gaggctggcg
      901 cccaggttct tgcgggcccg gaccaccggc accccgtcga tgatcgagtc catcgaggcg
      961 atgtagcccc actcgaaggc gcagtgggag atgtgttcgg actcgatcac cttgagggtc
     1021 tcgaagccgt tcctgcccag gaagaggatc tcgcccggcc ggacgtcccg gaggagttcg
     1081 aaatcgagcg tgttgaaggg gcgcgattcg gaggcgaccg ccagggcgcc gttcttgcgg
     1141 cccaggacca gcggcttgaa gccgtgcggg tcgcgggccg cgtagatgcc ctcccgggtg
     1201 agcagaagca cgcagtagga gccgcgcacc tgccgggcca gggcggccag cccgtcggcg
     1261 aaatcactgc cctgggcgat cagcttgccg agcagttcca cgtggtgctg ggtggagaag
     1321 gtgtggccgt tcttcttgag gtcgtcggtc aggcgctggg cgttgatgat gttgccggtg
     1381 aaggcgatgg caaaggggcc gaggcgggag tccagcagca tcggctgccg ttccttgagg
     1441 ctgacgtggc cgatgccgct ccgggcccgc agttcatcca gttcggccgg ggtgaaggtg
     1501 tggcggacat agcccttgtg ggtgaagatg cgcaggccgc gccgccaggt ggccaggccg
     1561 cagtagcgct ggccgcggtg ctggagtttg aagatgccgt agtagagttc gttgacgcag
     1621 ggctggtccg aatagatgcc gaagagtccg caattctcgt tcaggctgaa cattaatggc
     1681 tcccgagttt gtccaccgga ctgctgaggg gaaagggagg gggattgagc agaaattata
     1741 ccataaaaga gggtggttcg gcggttcggg attcagcgca gccggctttt aaaccggagg
     1801 acgcgctggt aggactggtc gatccgttcc gggctgaggc gccccgcgcg gacggccgcc
     1861 aggatggccc ggccggcccg ggcgggcgct tcgcggtccg gggtgaagta attggcgaag
     1921 agcagaatgt cgctgccggc atcaagcgcc cgcaccaccg cctcctcgaa ggagtaaaga
     1981 cggctgaccg cacccatctg gagatcatcg gtgatgatga cgccccggaa accgagtttt
     2041 ccccgcaggt tttccttgag atggcgggtg gagagggagg cgggccagtc gggatcgacg
     2101 ccggcctgga tgacatgggc ggtcaggact gcgtcggcca ggccggcccg gatgaggcgg
     2161 cggtagggtt ccagctcggc cgccgtccag gtggcggtga cgtcggtcag ccccagatgg
     2221 ctgtcttcgg cggcgctgcc gtggccggga taatgcttga gggcggtcaa caggccggcg
     2281 cggcggtgcg cccggatgaa agcggacgcg taccggacaa ccgtggccgg gtcggcggcg
     2341 aaggcccgtt ccttgcggcc gatggccggc gagtcgggat tgatattcag atcgactacc
     2401 ggcgccaggt tgaggttgaa cccgatttct ttcaggagac gggccatttc gagatagacg
     2461 ccgccggcgg ccgccggggt cagttcccgg gccgcccgcc gggcggaggg gaagtcccgg
     2521 aagccgcgtt tcgcgttgag acggccgacc ggaccgccct cctgatcaat ggccagcagc
     2581 agcggccggg gcacgggttg ggccttgatt tcggccacca gacggcgcag gtggtccggt
     2641 tcgccgaggt tgcggggaag gaagaggaga ccgccgattt cgccgcggct caactgctgc
     2701 cgaagccggg tgtcaagttc ggaaaagtcg cgtccttcga agccgaccat gatcaactgg
     2761 ccgatctttt cttccagcgt ggcggcgccg gccggccggc cggccagcag agcggcggcc
     2821 agcaacagaa attccaggaa cctgatccgg gttttcatcc attttaccgg ttatgttttt
     2881 gcggcgcggt tcttcaagga cgccggcggc gaaacggaaa tatgatagca taccctggcc
     2941 gtttccattg acttatttaa ccgccggtgt aaaatatctg gatatgaaca tcaaaaaatc
     3001 cgttcaaatg gttttctcga tggccgtctt gtttggcgcg ggtttaaatc cgctctgggc
     3061 cggctcgatt tcaggcacgg tttcctacgg gggcggcggc acggggaatg tgctgatcgg
     3121 cgccttcgac aatgccgcct tcttgggtga cccggccggc ttcgcggtgg tgacgacgcc
     3181 gctgccaaag gcatacagca tcaccaacct tcccaacggc agctattatc tgggcgcctt
     3241 cctggacctc aacggggata accagcggca ggcggaggag ccctccggat actacgccgg
     3301 caacccggtg gcggtgaccg cgctcgcccc caacccgacc ggccggaacc tcacccttac
     3361 cgttccggcg ctccaggagg agtaccggtt ccattcgctc ttattcggcc ctgacgatga
     3421 gaacggcttc cagcgcggcc gggtccggtt gtacaacaac gggcaggccg cggtcgagtt
     3481 cgaaggggag tccggcctga ccgccagctg gagccgggac gggcacggtt ttaccacctt
     3541 tctttccgag tcggacgggg aggtcgatac ccagttattt tacggggcgg gcgatttcat
     3601 gatcaccgcc agtggcggca tcgcccagga cgggccggat ccggccagcc gactctacga
     3661 ggcccaactg atggtcgcgg tgcggaagca ggcggtgact tacagcgcgg tcgacctggc
     3721 cggcgagtac aacctggtgg agatggaatg cggcggcgcg cacggcaaca gctgggtccg
     3781 gggcgtggtt tcggtggatg gcgacggcaa cctgaccttt gacctggttg actccgacgg
     3841 ccaggcgcac cagggcggct tcggaccgct ggccatcagc gccgatggga tcatcaccgc
     3901 ggacggccag cccgatttca agggagtgat gtcgccggag aagagggcgg tgctggccac
     3961 catgaccagc cgcccgggcg tttacagcct ccagatgctg gcccgccggt cgaacggttt
     4021 gaatggcgcc agcctgccgg gcggctacac cttcttcgaa gccaccgtct ccgacagcca
     4081 cagccaggtc gaggcccggc tgggcaccct ggaggtggag gccgatctcg actaccgggt
     4141 cctggagtgg tgccggatga agtacgactc gctcctttac caccaggggc gattcgcctt
     4201 cgtgccggag cagccgggcg tctttgcgcc ggtctccaat ccttacctgg ccgccttcgg
     4261 cggactggag gtcgggtccg gggtcaagct gctggcctcc agccggctgg gaccggccgg
     4321 cggttacgcg gtcgccatcg gtctttcggg gccggattat gcggccgacc ggacggccat
     4381 cgccgccctg accaccaact tcgtcgccca gtacaactcc aacccggcca acatcgggcc
     4441 attcttcgac aacgacttct atttcagggg gatgaacaag gccgccgcgg ttgcggatat
     4501 gctgccggag gcgggcgagg atccctacgt ggtcgtcagc gggttggaaa acatcatcct
     4561 ccgggaccgg gcgctgacct ttttccattt gcagatgcgt gacggcggcg cggccgggcc
     4621 agtgatcgaa gagggtgacg aggcctacga ttaccttaac tacctgaggc acaacggctc
     4681 ggcctggctc ttcttcggaa accagtcccg cagcggtccg ccccaggccg ccgccattaa
     4741 ctggagccgg tcggagacat cgaacggcgg ccgggtccag ttcgaggtgg aatcagacgg
     4801 gtttgtcatc aacgattgcc aggtggccgg gcccggggtg accgacccgg tccagttcaa
     4861 ttactggtcg gaccaggagg agggctacag ccaggcctcc ggtcaactgg aggatttcac
     4921 gccgcccctg ggcgcggtct acaccttccg ggtcgcttac gacaacaggg tcgaactaat
     4981 ccaggacagc gtcgagagcc ttttcacgac cgcgccccgg gtgactgcgc cggccgatgg
     5041 cgtcatcctg gccgatactt ccggaaaccg gcggccggac tttgcctgga cgatggagac
     5101 cgacggcgaa ccgcaggcca ccagctataa aatctggctg gaacggaccg gccaggactg
     5161 gcagcgcttc ttcgatttct acgaccttcc cttaagccgt ttcctggttt ccgggagcaa
     5221 ttaccgggcg gcctatgccg attaccagga ggagctgccg gacggggtaa gcgaactgtc
     5281 gccgggcaac tactccttcc gggtcaatct ttacgaccgg ttcaataacg gggccgaggg
     5341 gatctccgat ttcggaatcg ggcaggggcg cctttcgatc cgttcccaat actggaactg
     5401 ccagccggcg ccgggtgatt atttctataa ttacaacacg ccggtgaatg cccgggccga
     5461 cgccgtggtt gatcttggcg gctacacgcg ggcggcctgc accggctgga ccggctccgg
     5521 ggcggtggcc gcccaggggt tcgagaatga ggtcaatttc aatctgaccg ccaactcgac
     5581 cctggaatgg cactggtccg tccagcactg gctgagcaag agcgttaacg ggatcggctg
     5641 cgacgtgctg ggcgccgaca cctggattga ggccgggagc cgcgcgacgg tcacggcggt
     5701 ggccggagcg ggctggtttt tcgatcactg ggtgattgac ggcgtcgatc ggaccgacct
     5761 tgacacgccg gttttgagcc tgaccgtcga caacccggtc caggtccacg cgatcttcga
     5821 ggaattgtgg ccgctggaca tcgagatttc gccggccggt ttcgggagcg tcagtctgga
     5881 gccggccccg gccgcggcgc cgaattttta cccggccggg caactggtta acctgaccgc
     5941 ccggccgaat ggcgggtatg ccttccaggg ctggaacgga gttgacaccg tctccggcag
     6001 caccgcccag gtgacggttg gctggggcgg ccggtcggtg acggccttct tcaataacgg
     6061 cccctggctg acggtttcgg acacggcgct ggtcttcacc gccgacccgc aggccttgag
     6121 tctgaccctg accaacaccg gcggcgaaaa cctgggctgg gaggccgccg aggctattcc
     6181 ctggctgggc ctttccaaga cggccggcgc cctcgccaag gacggcggtt cggagattat
     6241 cacgctgacc cgcaccggcg gcgcggccgg cagccggggc gtggttactt tccagcagcc
     6301 cgccggttcc ttccagtcgc ccatcgaggt cacggttgtg attcccgcgc cgggcgacct
     6361 caacttcgac ctgacggttg acctggccga cgccgaactg gccttgcgga tactggttga
     6421 cccggagagt tacagcgagg cccagcggca ggccgccgac ctggacggca gcggcgcggt
     6481 ggatatcgag gacgtggtgg cggtataccg gctgctgctg gggatttgag aaggttggcg
     6541 ccgtttttca gtacccgccg ccgatccggc cgaccagggc ggccaggtcc cgggcggtca
     6601 cctggccgtc ctcatccagg tcggcggccg tccggtcacc ttcggcgggc aggccgaggc
     6661 tcatccgcag gaccgccagc gcgtcggcca ggtcgagttt tccgtcccgg ttgacgtcac
     6721 ccttcaggaa gaggtcgagc gcctggcgca ggttcagccg gccgccgctg atcagtttcc
     6781 cgctcaggcc ggccggcttg tccacccccg aaattatcct ttcccgccag cgtccggccg
     6841 gttcgtccgg gtaccgggcg gccaccaggg cgaccgcgcc ggccacgaag ggcgtcgcca
     6901 tcgaggtccc gtcccgggag gagtaggcgg aactcttgct gtaggtgacc atggtgagcg
     6961 ggttgccgag ggcccggatc cgctcgccgt cctcccgggt gatgaagacg gccggcaccc
     7021 agtcgccggg cgaccccagg gtccccagga tcaggccgcc gtccgggtcg ccggtcggga
     7081 tgttgttgta gatgacgacc gcggccgcgc cggcggcttg ggcgttggcg gtcttctcgg
     7141 agaagtagag ggtgccgcgc tggatgaggg cgaggttgcc ggagacctgg ggcgggaaat
     7201 cgcccgggtt gccgatgccg cagtagtaga tgggacgggt gatgccgtcg gtgtatccgg
     7261 agtactcaag cccctcggcc tcgctggtga cgccgccggc ggtgaccgcg gcggccatcg
     7321 gcaccgtcga aaggatgttg acgccgggcg cggccaggtg aaccgaggcc gccccgtaat
     7381 ttgagaagct ggccaggctg tcggcggcgg tggaagcggc caccgcgatg atgttgggca
     7441 gattgtagga ggccgggtag accgg
//
LOCUS       DAOQYN010000086         7417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_10368_length_7417_cov_4.491850, whole genome shotgun sequence.
ACCESSION   DAOQYN010000086 DAOQYN010000000
VERSION     DAOQYN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10368_length_7417_cov_4.491850"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKN80_04585"
     CDS             <1..715
                     /locus_tag="PKN80_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ35324.1"
                     /translation="SASEGGLLVTDSQEYCDRARLVRLFGEGEVGGDKPRDYNALTMG
                     WNYRISFFSAAMGLSLLNRFDQVRSAWQANCGRLSARLAGIPGVEPPFVPADRSHGFF
                     IYPIKFRPERLGMEVPVRPFIRAARRALQAEGVPVRFWQTKPVPGQALFQALEGYGKG
                     CPWKCHHRRPGLRYDPAEYPVTNEVISRSLYLLKMSGFNPPNTPELMDRYADAFQKVL
                     VENRDELAVLVKEEERTEK"
     gene            712..1563
                     /locus_tag="PKN80_04590"
     CDS             712..1563
                     /locus_tag="PKN80_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNQ35325.1"
                     /translation="MRKSRGKYYLPELTRREVQAIADRAILLIPIATIEQHGPHLPMH
                     TDIDNCEGLVRGVVEKLQPRLPVYRSVSFWYAPAGAGAMVGFPGALWMRKEIFKEVLN
                     DHLLSYLRGGFKKIVLISGHGGGTHIWIPEVVRNLRKRVADMNWSGDWQVPADARVIT
                     FNWLAFLAEFAASECARIRKNPAGSDWHGGDVETALQLYLRPDLVDMSQAKRGTIQRP
                     TRFGASDIFNWHRQLVIEGYPSKSDPGEVDRVYGDPTLATRELGRRVYRLAVRKMAEL
                     VEEFASL"
     gene            1560..2345
                     /locus_tag="PKN80_04595"
     CDS             1560..2345
                     /locus_tag="PKN80_04595"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ35326.1"
                     /translation="MKEKKKRPAGPLRPRPGSLEFLDANLMLGRWTEQALLFHDRAGL
                     EAELARHRVSGGLVYHSWARHYDPAEGNRQLWRELAGAAGLYPAAVLLPFATGEFKPE
                     ELAGAVRAKRIRAVRLFPVEHRYTLADWNCASLFDFLAEYRLPTFLELNQADWNEAAR
                     ILGRYPGLPLVIAATGYRHGRILYPLLEKFSNLYLETSAYVTYEGIEDICRRFGSGRL
                     LFGSRAPFMDLGGALARIIYAGIPAADRQAIAAGNLKRLLGVQ"
     gene            2342..3187
                     /locus_tag="PKN80_04600"
     CDS             2342..3187
                     /locus_tag="PKN80_04600"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ35327.1"
                     /translation="MKLHEAARSGEPVEGLEIIDCHAHLGPWFNFPIAGDPSAAGMVR
                     VMDRCGIRAACIVPHLGIGPDFRGSNDLMLEALRKFPGRFFGLAIYNPHFPEESLADL
                     ERSFRAGAIGIKIHPDTHLYPVTGRNYEPAWKFARQHRSFVLSHTWGDQRCHPDFFLE
                     IARSYPSVRFILGHSGGGPGGHEAAIRVAVKAANIFLDTCGSAIPGWWLERLADGAGI
                     DRLLLGTDCPFIDPRPAVGRILMGRFSEAEKRAILGGNFRKHFLAGPAGRKRGRTPGA
                     RSSKE"
     gene            3194..5500
                     /locus_tag="PKN80_04605"
     CDS             3194..5500
                     /locus_tag="PKN80_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35328.1"
                     /translation="MADIALMWHGSLQWGRRQVSEWHFLAEVYQATLEVLEKHPKVHL
                     TLQFGGQDLEWFSHTHPELIGQVRRLMEAGQAEVSVGGYSHNFQGAVPELNLANLVFA
                     QRVCRDLFGVAADGYWPAESMLNRGMPGSLLEAGFKYTMFDSLNVENAAELELGVTGH
                     TYRLRGAFGIEIPGLPTSRFPDAGSRGEGAALQRFSQNPAAYDYDPAVVLDRLGRTPL
                     VVECSDWEHFNIHQEKVQQVQMGTYHIGLENKVDPEKIAAYDRILTLAEAAGHRFVAC
                     REALALHPPAGALELIDGRDYTKNDLTRLVDACAWYKSGKGGLSERKDPHRLSDQESY
                     SYSFGGARAWQFLKELAGAGAPVSEAELASRTKDILISFCSAHRSGGEDFTVKQQLLD
                     RTDRVIESAQRHLETLAGEGELLLPAGLPAGLWPLPVNLPGRPPAGYELRSGRRLAGR
                     AWLEAAATHTAGLDRSHQLWVAGPVPAGRYRLEASARPAGYGTGLKVRESETGLRLEG
                     RSGYLGLDRFNGGGLAGWELRSFAPFARLGRLEFNSRWQEAPLVDEVPARFTWRTLPF
                     GVQVRADFRIGGLDFRRTWLVANDLATCQMRLHCFHRSFSYLAPAVYRLNPAAFGRRG
                     RFLMVAEGGTRVAAEPLARFHQIHHLGSELVGFAAAGGLAGLAVNRLVSSPILRRRPL
                     SGTFILMDSRPIGAADNAYPYQFAREPELELTLRLAVGSEPLPTFRAWQSFQDRPALL
                     RFGRPPLQPSRLEWTKEIAGLTEVNPYF"
     gene            5512..>7417
                     /locus_tag="PKN80_04610"
     CDS             5512..>7417
                     /locus_tag="PKN80_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35329.1"
                     /translation="MKRIWLLGSLAFFLAAPRVSGADLPGGARVLFYLPFENELTAIT
                     PARELEPEAGVDVVDDLSQSVTIGELARGKQIVVGDKQISGGDSRIAYEDGLRGRSVR
                     LRTRLTYAAPWLKEQPEGTITLWYRNPNWRVTTWNVPAMLTTAQPDRAKTSAFQTPIS
                     WWIFNRLTAPPEQGNSRWYHWTIEFDEHPRNISPREKDDDRWHFWALRWNRAAGLLEV
                     IDDGQVRDISRYANRLEPGRDFWLETYRGHTTQVYEDVGHIKFEEVFLGSLHLARFCE
                     VGLDEIFVLDKALSREELAAACRRGLQGQPTWPAEAVPKPAQRLSEGLDLAALDPARP
                     GLPAEVDWSSQGAVAERSATQEKVCLNGYWRFQPVADRFTRPDPQQWSYVRLPGLWGE
                     KSYLLDPAKLRARANWNGKPLAGYELVWLEREVEVPANQAGRRFFLSFDRVDEDLTRF
                     DLYINGRFVRRFRNYGPGRTDITGWIQPGQRNRLLLGVEKKDRFLFDLYLESRQLTKV
                     SVHDPYVVSEYRKKAVTVQFDLENHTDQPQTYTYGVEFCEWPSGRQVHTLKGATLTIG
                     PKRSEVASVSGPWPNPHCWSIYDPFLHTARVLVRDGRGRTLDTGLPVRFGYRELWSEN
                     GWLFLNGVKMQ"
BASE COUNT         1145 a         2585 c         2494 g         1193 t
ORIGIN      
        1 cagcgccagc gagggcggcc tgctggtgac cgattcccag gagtattgcg accgggcccg
       61 gctggtccgt ctcttcggcg agggcgaggt gggcggggat aagccgcgcg attacaacgc
      121 cctgaccatg ggctggaact accggatcag tttcttctcg gccgccatgg gcctgagcct
      181 gctgaaccga ttcgaccagg tgcggtcggc ctggcaggcc aactgcggcc gcctctcggc
      241 ccggctggcc gggatcccgg gcgtcgagcc gcccttcgtt cccgccgacc ggagccacgg
      301 tttcttcatc tacccgatca agttccggcc ggagcggctg gggatggagg tgccggtgcg
      361 ccccttcatc cgggcggccc gccgggcgct ccaggccgag ggggtgccgg tccgcttctg
      421 gcagaccaaa ccggtgccgg gccaggcgct cttccaggcg ctcgagggtt acggaaaggg
      481 ctgtccctgg aagtgccacc accgccggcc gggactccgg tacgacccgg ccgagtaccc
      541 ggtcaccaac gaggtcatct cccggtccct ctacctgctg aagatgagcg gcttcaatcc
      601 gcccaacacc ccggaactga tggaccgtta cgccgacgct ttccagaagg tgctggtcga
      661 gaaccgcgac gaactcgcgg tactggtcaa ggaagaagaa aggacggaaa aatgaggaag
      721 agccggggca agtactacct gccggagctg acccgccgcg aggtccaggc catcgccgac
      781 cgggcgatcc tgctgatccc gatcgccacc atcgagcagc acggcccgca cctgccgatg
      841 cacaccgaca ttgacaactg cgaggggctg gtccggggcg tggtcgagaa actccagccg
      901 cgcctgccgg tctaccgcag cgtctccttc tggtacgcgc cggccggcgc cggggccatg
      961 gtgggatttc cgggggccct ctggatgcgc aaggagatct tcaaggaggt cctgaacgac
     1021 cacctgctca gctacctgcg gggcggcttc aagaagatcg tgctgatcag cggccacggc
     1081 ggcggtaccc acatctggat accggaggtg gtccgcaacc tgcgcaagcg ggtggccgac
     1141 atgaactgga gcggggactg gcaggtgccg gccgacgccc gggtgatcac cttcaactgg
     1201 ctggccttcc tggccgagtt cgccgcctcc gagtgcgccc ggatccggaa gaacccggcc
     1261 ggctccgact ggcacggcgg cgatgtcgag accgccctcc agctttacct gcggccggac
     1321 ctggtggaca tgagccaggc gaaaaggggg accatccagc ggcccacccg tttcggcgcc
     1381 tcggacatct tcaactggca ccgccagctg gtcatcgagg gttatccgtc caagagcgac
     1441 ccgggcgagg tggaccgggt ttacggggac ccgaccctgg ccacccggga actgggccga
     1501 cgcgtctatc gactggccgt gcgcaagatg gccgaactgg ttgaggagtt cgcctcgcta
     1561 tgaaagaaaa gaagaagcgg ccggccggcc cgctccggcc gcggccggga tccctggaat
     1621 ttctcgacgc caacctgatg ctgggccgct ggaccgagca ggcactcctc tttcacgacc
     1681 gggccggcct cgaggcggag ctggcccgac accgggtctc cggtggcctg gtctaccata
     1741 gctgggcccg ccattacgac ccggccgagg ggaaccgcca gctctggcgc gagctggccg
     1801 gcgcggccgg tctttacccg gcggcggtgc tgcttccctt cgccaccggc gaattcaagc
     1861 cggaggaact ggcaggggcg gtccgggcga agcgcatccg ggcggtccgc ctcttcccgg
     1921 tcgagcaccg ctacacgctg gccgactgga actgcgcttc cctcttcgat ttcctggccg
     1981 agtatcggct accgaccttc ctggaactga accaggctga ctggaacgag gcggcccgga
     2041 tcctgggccg ttatcccggc ctgcccctgg tcatcgccgc caccggctac cggcacggcc
     2101 ggattctcta cccgctcctg gagaagtttt ccaacctcta cctggagacc tcggcctatg
     2161 tcacctacga gggcatcgag gatatctgcc gccgcttcgg cagcggccgc ctgctcttcg
     2221 gttcccgggc gcccttcatg gacctgggcg gcgccctggc ccggatcatt tacgccggta
     2281 tcccggcggc cgaccggcag gcgatcgcgg ccgggaacct gaagcgtttg ctgggggtgc
     2341 aatgaagctg cacgaagcgg cccggtccgg cgaaccggtt gagggcctgg agatcatcga
     2401 ctgccacgcc catctcggtc cctggttcaa cttcccgatc gccggggacc cttcggcggc
     2461 ggggatggtt agggtgatgg accgatgcgg gataagggcg gcctgcatcg tgccccacct
     2521 gggcatcggc cccgatttcc ggggcagcaa cgacctgatg ctggaagcgc tccggaagtt
     2581 cccgggccgc ttcttcggcc tggccatcta caatccacat ttcccggagg agtcgctagc
     2641 cgacctcgag cgctccttcc gggccggcgc gatcgggatc aagatccacc cggacacgca
     2701 cctttacccg gttaccggcc ggaattacga gccggcctgg aagttcgccc gccaacaccg
     2761 gagtttcgtg ctcagccata cctggggtga ccagcgctgc cacccggact ttttcctcga
     2821 gatcgcccgc agctacccgt cggtccgttt tatcctcgga cacagcggcg gtgggcccgg
     2881 cggtcacgag gcggccatcc gggtggccgt gaaggccgcc aacatcttcc tggacacctg
     2941 cggcagcgcc atcccgggct ggtggctgga gcggctggcc gacggcgccg gcatcgaccg
     3001 cctgctgctg gggaccgatt gtcccttcat cgacccgcgg ccggccgtcg gccggatcct
     3061 gatgggccgc ttctccgagg cggagaaacg ggccatcctg ggcggcaact tccggaaaca
     3121 ctttctcgcc ggccccgccg gccgcaagcg cggccggacc ccgggcgccc ggtcatccaa
     3181 ggagtaaaag ataatggccg atatcgccct gatgtggcac ggctccctcc agtgggggcg
     3241 ccgccaggtc tccgagtggc acttcctggc cgaggtttat caggccaccc tggaggtgct
     3301 ggagaagcat ccgaaggttc acctgaccct gcagttcggc ggccaggacc tggaatggtt
     3361 ctcccacacc catcccgagc tgatcgggca ggtccggagg ttaatggagg ccggccaggc
     3421 cgaggtttcg gtgggcggct actcgcacaa cttccagggc gcggttccgg aactgaacct
     3481 ggccaacctg gtcttcgccc agcgggtctg ccgggacctc tttggagtgg cggccgacgg
     3541 ctactggccg gcggaatcga tgctcaaccg cggcatgccc ggatccctgc tggaggccgg
     3601 gttcaagtac acgatgttcg acagcctgaa cgtcgaaaac gccgccgagc tggaactggg
     3661 agttaccggc cacacctacc gcctgcgcgg ggccttcggg attgagattc cgggcctgcc
     3721 gaccagccgc ttccctgacg ccggttcccg gggcgagggg gcggcgctcc agcgtttcag
     3781 ccagaacccg gccgcctatg attacgatcc ggccgtggtg ctggaccggc tgggccggac
     3841 gcccctggtg gtcgagtgct ccgactggga gcatttcaac attcaccagg agaaggttca
     3901 gcaggtccag atgggcacct accacatcgg cctggagaat aaagttgatc cggagaagat
     3961 cgccgcctac gaccggattc tgaccctggc cgaggcggcc ggccaccgtt tcgtcgcctg
     4021 ccgggaggcg ctggcgctcc acccgccggc cggcgcgctg gaactgatcg acggccgcga
     4081 ttacaccaag aacgacctga cccggctggt ggacgcctgc gcctggtaca agtcggggaa
     4141 gggcggcctt tcggagcgga aggatccgca ccggctgagc gaccaggaga gttacagcta
     4201 ctccttcggc ggcgcccggg cctggcagtt tttgaaggaa ctggccgggg ccggcgcgcc
     4261 ggtctccgag gcggagctgg caagccggac caaggacatc ctgatcagtt tctgcagcgc
     4321 ccaccggtcc ggcggcgagg atttcaccgt gaagcagcag ctgctggacc ggaccgaccg
     4381 ggtgatcgag agcgcgcagc gccacctgga gaccctggcc ggggagggcg agctcttgct
     4441 gccggccggc ctgccggccg gactctggcc cctgccggtc aatcttccgg gccggccgcc
     4501 ggccggctat gagctccgtt ccggccggcg cctggcgggc cgcgcctggc tggaagcggc
     4561 cgccacccat accgccggcc tggaccggag ccaccagctc tgggtggccg gcccggtccc
     4621 ggccggccgc taccggctgg aagcctcggc ccggcccgcc ggttacggca ccggtctgaa
     4681 ggtgcgcgag tcggagaccg gcctgcgcct ggagggccgg tccggctacc tcggcctgga
     4741 ccgtttcaac ggcggcggcc tggccggctg ggagctgcgt tccttcgccc ccttcgcccg
     4801 gctgggccgg ctggaattca acagccgctg gcaggaggcg cccctggtgg atgaggtgcc
     4861 ggcccgcttc acctggcgga cactcccctt cggcgtccag gtccgggccg atttccggat
     4921 cggcggcctc gacttccggc ggacctggct ggtggccaac gacctggcga cctgccagat
     4981 gcgcctccac tgctttcacc gttccttcag ttacctggcc ccggcggttt accggctcaa
     5041 cccggccgcc ttcggccggc gcggccgctt cctgatggtg gccgagggcg gcacccgggt
     5101 ggctgccgag ccgctggccc ggtttcacca gatccatcac ctcggttcgg aactggtcgg
     5161 cttcgcggcg gccggcggcc tggccggcct ggccgtcaac cggctggtct cctcgccgat
     5221 actgcgccgc cggccgctct ccgggacctt catcctgatg gattcgcggc cgatcggcgc
     5281 cgccgacaac gcttatccct accagtttgc ccgggagccg gagctggaac tgaccctgcg
     5341 cctggcggtc ggttccgagc cgctcccgac cttccgggcc tggcagtcct tccaggaccg
     5401 cccggccctg ctgcggttcg ggcggccgcc cctccagccg agccggctgg agtggacgaa
     5461 agagatcgcc ggcttgaccg aggtcaaccc ttacttctga cggaggcgga aatgaagaga
     5521 atctggctgt tgggttcgct ggccttcttt cttgcggcgc cgcgggtatc cggcgccgac
     5581 ctgcccggcg gcgcccgggt gctcttctat ctccccttcg agaacgagct gaccgccatc
     5641 accccggccc gggagctgga accggaggcc ggcgtcgacg tggtggacga cctgagccag
     5701 agcgtgacca tcggcgaact ggccaggggc aaacagatcg tggtgggcga caagcagatc
     5761 tccggcggcg attcccggat tgcctacgag gacgggctgc gcggccggtc ggtgcgcctg
     5821 cgcacccggc tcacctacgc ggccccctgg ctgaaggagc agcccgaggg gaccatcacc
     5881 ctctggtatc ggaaccccaa ctggcgggtg acgacctgga acgtgccggc catgctgacc
     5941 accgcccagc cggaccgggc caagacctcg gccttccaga cgcccatctc ctggtggatc
     6001 ttcaaccggc tcaccgcgcc gccggagcag ggcaacagcc gctggtacca ctggaccatc
     6061 gagtttgacg agcacccgcg caatatttcc ccgcgcgaaa aggacgacga ccggtggcac
     6121 ttctgggcgc tgcgctggaa ccgggccgcc ggcctgctgg aggtcatcga cgacggccag
     6181 gtccgggata tctccaggta tgccaaccgg ctggagccgg ggcgcgattt ctggctcgag
     6241 acctaccgcg gccacaccac ccaggtctac gaggacgtcg gccacatcaa gttcgaagag
     6301 gtcttcctgg gcagcctcca cctggcccgt ttctgcgagg tggggctgga cgagatcttc
     6361 gtgctcgaca aggccctctc ccgcgaggaa ctggccgccg cctgccggcg cggcctccag
     6421 gggcagccga cctggccggc ggaggcggtt cccaagccgg cccagcgcct ctccgaggga
     6481 cttgatttgg ccgccctcga tccggcccgg cccggccttc cggccgaggt ggattggagc
     6541 agccaggggg cggtcgccga acggagcgcc acccaggaga aggtctgcct gaacggctac
     6601 tggcgcttcc agccggtcgc cgaccgtttc acccggcccg atccgcagca atggtcttat
     6661 gtccggctgc ccggtctctg gggcgaaaag tcctacctgc tcgacccggc caagctgcgc
     6721 gcccgggcca actggaacgg caagccgctg gccggttacg aactggtctg gctggaacgg
     6781 gaggtggaag tcccggccaa ccaggcgggc cgccgtttct tcttaagttt cgaccgggtc
     6841 gatgaggacc tgactcgttt cgacctctac atcaacggcc gcttcgtgcg ccgtttccgc
     6901 aattacgggc cgggccggac cgacatcacc ggctggatcc agccgggcca gcggaaccgg
     6961 ctgctcctgg gcgtcgaaaa gaaggaccgg ttcctcttcg acctttacct ggagtcgcgt
     7021 cagttgacca aggtttcggt ccacgatccc tatgtcgtct ccgagtaccg caagaaggcg
     7081 gtcaccgtcc agttcgacct ggaaaaccac accgaccagc cgcagaccta cacctacggg
     7141 gtcgagttct gcgagtggcc ctcgggccgc caggtccaca ccctgaaggg cgcgaccctg
     7201 accatcgggc cgaagcggtc cgaggtggcc tcggtcagcg gcccctggcc caacccgcac
     7261 tgctggtcca tttacgatcc cttcctccac acggcccggg tcctggtccg ggacggccgg
     7321 gggcggacgc tggataccgg gctgccggtc cgtttcggct accgcgaact ctggagcgag
     7381 aacggctggc tcttcctgaa cggggtcaag atgcaga
//
LOCUS       DAOQYN010000087         7388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_10432_length_7388_cov_3.948589, whole genome shotgun sequence.
ACCESSION   DAOQYN010000087 DAOQYN010000000
VERSION     DAOQYN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10432_length_7388_cov_3.948589"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..371
                     /gene="rpsJ"
                     /locus_tag="PKN80_04615"
     CDS             66..371
                     /gene="rpsJ"
                     /locus_tag="PKN80_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNQ35330.1"
                     /translation="MSQKLRIKLRSYDHRLLDQSVREIIEIGKRTGENLAGPIPLPTK
                     REIFTVQRSTFVHKKSREQFEIRIHRRLIDIINPSARIVDALTKLSLPAGVDVEIKS"
     gene            476..1099
                     /gene="rplC"
                     /locus_tag="PKN80_04620"
     CDS             476..1099
                     /gene="rplC"
                     /locus_tag="PKN80_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNQ35331.1"
                     /translation="MALGIIGRKVGMTRIFNADGRTVPVTVVQAGPCPVVARREPGNG
                     GYASLQLGYGAGRKVSKPLAGHFKKAGLAPTRILKEFRVSNEQMEKFPSGSQVTVEIF
                     EVGERVAVTGRTIGKGFQGVVKRHGFKGGAATHGSMSHRAPGSIGGSNPDRVTKGHRM
                     GGRMGGVNLTVRGLEVVGIDKEKNLLLLKGAVPGAEDGLLQVKKIKG"
     gene            1123..1743
                     /gene="rplD"
                     /locus_tag="PKN80_04625"
     CDS             1123..1743
                     /gene="rplD"
                     /locus_tag="PKN80_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNQ35332.1"
                     /translation="MEIKVYNLAGEVVGREEFDTSGLAPVNQKLLYYAVNAYRANQRQ
                     GTASTKTRGEVSGGGHKPWRQKGTGRARVGSNRSPLWRKGGITFGPRPRDYRVELPAK
                     MRRRAQAEAIKAKIMAGSVGLLENLQLAEPKTKLVGAFLKKSGICGKILLFAPRDAAD
                     FRRAGRNIPGMKFADLRTADFYSILNADYLIAPHSDWGALQERLKA"
     gene            1752..2030
                     /gene="rplW"
                     /locus_tag="PKN80_04630"
     CDS             1752..2030
                     /gene="rplW"
                     /locus_tag="PKN80_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNQ35333.1"
                     /translation="MTANDIIRYPLVTEKDTMYLQPLNKYAFAVQVGATAPEIGKAVE
                     KLYKVKVVAVHVLNYQGKTRRMRNRAGRRPDWKKAIVTLRKGDKIEFA"
     gene            2017..2835
                     /gene="rplB"
                     /locus_tag="PKN80_04635"
     CDS             2017..2835
                     /gene="rplB"
                     /locus_tag="PKN80_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNQ35334.1"
                     /translation="MSLRKKRPVTPASRFSTLPDFAEITRKSPERRLLETRRRRGGRN
                     SQGRVTVRHRGGGHRHHYRIIDFVGGKTLTGRVLGIEYDPNRSARIALVVFPDGEKRY
                     ILATQEMKDGALVSCGAEAQPASGNRLPLRSIPEGSNVCCLELRPGLGGKLVRSAGAT
                     AVLMAKTEKAAQIKLPSGEIRLFDLDCQATIGSISNSEHRYESLGKAGRVRWTGRRPK
                     VRGVVMNPVDHPLGGGEGKSSGGRPPTSPWGKPEGVKTRDRRRHSRKMIVKRRK"
     gene            2866..3150
                     /gene="rpsS"
                     /locus_tag="PKN80_04640"
     CDS             2866..3150
                     /gene="rpsS"
                     /locus_tag="PKN80_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNQ35335.1"
                     /translation="MSRSLKKGPQVDPKLLKKIAAVRASGRKEVIKTWRRSSQISPEF
                     VGMTLGIHNGRTFIQVYITENMVGHRLGEFSPTRVFRHHGAHTERSLSKT"
     gene            3177..3521
                     /gene="rplV"
                     /locus_tag="PKN80_04645"
     CDS             3177..3521
                     /gene="rplV"
                     /locus_tag="PKN80_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNQ35336.1"
                     /translation="MAEVIAKSKYIRISPQKAQEIVLLLRGKTPQEALQVLKNVYKRP
                     ARHVAKTIASALANVRSRKLEESNFRFKTLVVEQGPMLKRSRAASMGRAVSVLHRTSH
                     IKVILTDEWDKK"
     gene            3506..4141
                     /gene="rpsC"
                     /locus_tag="PKN80_04650"
     CDS             3506..4141
                     /gene="rpsC"
                     /locus_tag="PKN80_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNQ35337.1"
                     /translation="MGQKVIPTALRLGYIEGWRSNWFQHRRLGPSILEDYKVRAYLQK
                     RFPESGITRIDIDRLVDMIRIKIRASRPGLIIGRKGAEINNVSKELQALCNKNVTVDV
                     SEVTNSSVDAAFLAQSIAQQISRRGTSYRFVCKNTIENAMQRGANGVKIKISGRLAGA
                     EISRSESYKDGTIPLHTLSAIIDYAQTTAHTTYGAIGVKVWLYRGDAKEKA"
     gene            4159..4566
                     /gene="rplP"
                     /locus_tag="PKN80_04655"
     CDS             4159..4566
                     /gene="rplP"
                     /locus_tag="PKN80_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNQ35338.1"
                     /translation="MPLIPKRVKHRHMHGGRTRGRALTGNRLSFGEYGLLALEPGYFN
                     ERQLEAARVAITHHLKGKGKLWIRVFPDRPITGKPAETRMGKGKGDLKCWTARILPGR
                     VIVELTGVDLELARSALKLAAGKLPLPTRVISR"
     gene            4563..4763
                     /gene="rpmC"
                     /locus_tag="PKN80_04660"
     CDS             4563..4763
                     /gene="rpmC"
                     /locus_tag="PKN80_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017999563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNQ35339.1"
                     /translation="MKRNDFQELQKLGPLELRERVDSLKAEYYRLRFQQKMGQLKETA
                     SLGSVRRDIARVMTLLNQKQKG"
     gene            4787..5032
                     /gene="rpsQ"
                     /locus_tag="PKN80_04665"
     CDS             4787..5032
                     /gene="rpsQ"
                     /locus_tag="PKN80_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNQ35340.1"
                     /translation="MKRQESGTVVSDRMDKTRVVVVERLIRHPLYQREMRRRTRYKVH
                     DPKNESHTGDRVLIEEFRPISRDKSWRLLSIIKKGSE"
     gene            5034..5402
                     /gene="rplN"
                     /locus_tag="PKN80_04670"
     CDS             5034..5402
                     /gene="rplN"
                     /locus_tag="PKN80_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNQ35341.1"
                     /translation="MVQLRTILEVADNTGAKKIMCFGVLGGSRKRVARVGDIIKASVK
                     DALPNGTVKRREVVRAVVVRTKAHIRRPDGSYLSFDHNSAVIIDDDGNPRGTRVFGPV
                     ARELRDKNFTKIISLAPEVL"
     gene            5399..5725
                     /gene="rplX"
                     /locus_tag="PKN80_04675"
     CDS             5399..5725
                     /gene="rplX"
                     /locus_tag="PKN80_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNQ35342.1"
                     /translation="MKLRKGDFVLVAKGKDAGKKGRVLRVYPEANRALVEGINFVKRH
                     LRPGRVERQGGIVQKEMPVAIPNLRYFCLKCNQATRVGFKFLGDSKVRCCRKCGEIIE
                     TKSTTA"
     gene            5739..6284
                     /gene="rplE"
                     /locus_tag="PKN80_04680"
     CDS             5739..6284
                     /gene="rplE"
                     /locus_tag="PKN80_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNQ35343.1"
                     /translation="MARLLDKYKKEIIARLTEQFKYSNQMAVPKLTKIVVSVGLGKIG
                     KEEKVVEEILGGLTAITGQKPVMRRARKSIAGFNLRTNDLVGAFVTLRGQRMYEFLDR
                     LVSFALPRVRDFRGLPKTGFDRAGNYSFGLNEQTIFPEIDFNKVTKVFGMNINLVTSA
                     RNRQEAEPLLTMLGLPLEKEE"
     gene            6291..6473
                     /locus_tag="PKN80_04685"
     CDS             6291..6473
                     /locus_tag="PKN80_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNQ35344.1"
                     /translation="MAKKSMIIKNQHPKYKVRQRNRCCICGRPRGYMRRFSLCRICFR
                     ELASKGFIPGVVKASW"
     gene            6501..6902
                     /gene="rpsH"
                     /locus_tag="PKN80_04690"
     CDS             6501..6902
                     /gene="rpsH"
                     /locus_tag="PKN80_04690"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNQ35345.1"
                     /translation="MVTDPIADLLTRIKNGGLARKPVIRIPYSVFKEDILKVLADQGF
                     IASYAVEADGKKKNLSVWLKYRPNSTRSVIQAVEIKSRASQRIYVTQQDLKRLIRGRQ
                     KIGILSTSQGVISHRDALRKRVGGELLAVIW"
     gene            6933..>7388
                     /gene="rplF"
                     /locus_tag="PKN80_04695"
     CDS             6933..>7388
                     /gene="rplF"
                     /locus_tag="PKN80_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNQ35346.1"
                     /translation="MAKQGKRPVRIPDGVKASLEGTVLKMSGPMGNLGLEVPPVLSVV
                     LEKGSIRVDFRSAAGNGERKNETENESLRSLYRALINSRMKAVKEGITKQLEVIGVGY
                     QAELKGKNLVLKIGFSHPVSVPVPSEIKVKVEKNLITLAGSDPQQVGDFA"
BASE COUNT         1616 a         2192 c         2354 g         1226 t
ORIGIN      
        1 cgcgagggcg gccacaccgt cggcgccggc gcggtcaccg acgtcatcga gtgagcggga
       61 aaaagatgag ccagaaactg cgcatcaagc tgcgttccta cgaccaccgg ctgctggacc
      121 agtcggtccg cgagatcatc gagatcggca agcggaccgg cgaaaatctg gccgggccga
      181 tcccgctgcc gaccaagcgc gagatcttca cggtccagag atcgaccttc gtgcacaaga
      241 aaagccgcga gcagtttgag atcagaatcc accgccgttt gatcgacatc atcaatccct
      301 ccgcccgaat tgtcgacgcg ctcaccaagc tgagcctgcc agccggggtt gacgtagaga
      361 tcaaatcgta gtataataaa aacttttgac gggatagatt ttctcaaaat attcgaatcg
      421 cggtcaaaga ggggagcact cgctggcttc tcctcttttt tttcgggata agcaaatggc
      481 tcttggaata atcggacgca aggtgggcat gacccgcatt ttcaacgcgg acggccggac
      541 ggtcccggtg accgtggtgc aggccggccc ctgcccggtg gtggcccggc gcgaacccgg
      601 caacgggggg tacgcctcgc ttcagctggg atacggcgcc ggccggaagg tcagcaagcc
      661 gctggccggc cacttcaaga aggccggcct tgcgcccacc cgcatcctca aggaattccg
      721 ggtttcgaac gaacagatgg agaagtttcc ctccggcagc caggtgaccg tcgagatctt
      781 cgaggtcgga gagagagtgg cggttaccgg ccggacgatc ggcaagggct tccagggcgt
      841 ggtcaagcgc cacggtttca agggcggcgc ggcaacccac ggttcgatgt cgcaccgcgc
      901 gccgggttcg atcggcggca gcaaccccga ccgggtcacc aagggacacc ggatgggcgg
      961 ccggatgggc ggcgtcaacc tgaccgttcg ggggctggaa gtggtcggca tcgacaaaga
     1021 gaagaacctg ctcctgctca agggggcggt gcccggggcc gaggacggcc tgctccaggt
     1081 taagaaaatc aagggttgat cccgggacgc gctcaaataa aaatggaaat caaggtttac
     1141 aatctggccg gcgaggtggt gggccgcgag gagtttgaca cttccgggct ggcgccggtc
     1201 aaccagaaac tgctctatta cgcggtgaac gcctaccggg ccaaccagcg gcagggtacg
     1261 gcctccacca agacccgcgg tgaagtctcc gggggcggcc ataaaccctg gcgccagaag
     1321 ggtaccggcc gggcccgggt cggttccaac cgttccccgc tctggcgcaa gggcggaatc
     1381 acctttggtc cccggccgcg cgattaccgg gtggaacttc cggccaaaat gcggagacga
     1441 gcccaggccg aggccatcaa ggcgaagatc atggccggca gcgtcggcct gctggagaat
     1501 cttcagctgg ccgaacccaa gacgaagctg gtcggcgcct ttttgaagaa aagcggtatc
     1561 tgtggtaaaa tattactttt tgccccccgc gacgccgccg atttccgcag agcggggcgc
     1621 aacatccccg gcatgaagtt cgccgacctc cggacagccg acttttacag cattcttaac
     1681 gccgactacc tgattgcccc gcattccgac tggggcgcgc tccaggaacg gctcaaagcc
     1741 tgagttccgc catgaccgct aatgacatta tcaggtatcc gctggtgacc gagaaggaca
     1801 ccatgtacct gcagccgttg aacaagtacg cgttcgcggt tcaggtcggg gccaccgcgc
     1861 cggagatcgg caaggcggtc gagaagcttt acaaggtcaa ggtggtggcg gttcacgtct
     1921 taaactacca gggcaagacg agacggatgc gcaaccgggc cggccgccgg ccggactgga
     1981 agaaggccat cgtcaccctg cgcaaaggag ataaaattga gtttgcgtaa gaagcgtccg
     2041 gtaactccgg ccagccgctt ttcgacgctg ccggactttg ccgagatcac ccggaaaagc
     2101 ccggagcggc gtttgttgga aacccgccgc cgccggggcg gccgcaacag ccagggccgg
     2161 gttaccgtgc gccatcgggg cggcggccac cggcaccatt accggatcat tgatttcgtg
     2221 ggcggcaaga ccctgacggg ccgggtgctg ggcatcgagt acgatccgaa ccggagcgcc
     2281 cggatcgccc tggtcgtctt cccggacggc gaaaagcgtt acattctggc cacccaggag
     2341 atgaaggacg gcgccctggt ttcctgcggc gccgaagccc agccggcatc gggtaaccgc
     2401 ctgccgctgc gctcgatccc ggaagggagc aacgtctgct gcctggaatt gcgcccgggc
     2461 ctggggggca agctggtccg ctcggccggc gccaccgcgg ttctgatggc caagaccgaa
     2521 aaggcggccc agatcaagct gccctcgggc gaaatacgac tttttgacct ggattgccag
     2581 gccaccatcg gttccatcag caattccgag catcgttacg agtcgctggg caaggccggc
     2641 cgggtccgct ggactgggcg ccggccgaag gtgcgcggcg tggttatgaa cccggtcgac
     2701 cacccgctgg gcggcggcga gggcaagagt tccggcggcc ggccgccgac ctcgccctgg
     2761 ggtaagccgg aaggggtcaa gacccgcgac cggcgccgcc attcccggaa aatgatcgtc
     2821 aagcgccgca agtaaccggg cgatcttacg gaggcaggag aaaagatgtc tcgttcactc
     2881 aagaaagggc cgcaggtaga cccgaagctg ctcaagaaga tcgcggcggt ccgggccagc
     2941 ggccggaagg aagtcatcaa aacctggcgg cgttccagcc agatcagccc ggagttcgtc
     3001 ggcatgacgc tgggcattca caacggccgg actttcatcc aggtttatat tactgaaaac
     3061 atggtgggcc accggctcgg ggagttttcg ccgacccggg tcttccgcca tcacggcgcc
     3121 cacacggaac ggtcgctttc caagacctaa aaatacatca atccggacag agaacgatgg
     3181 ccgaagtaat cgcaaaatcg aagtacatca ggatcagccc gcagaaggcg caggagatcg
     3241 tcctgctgct tcgcggcaag acgccccagg aggcgctcca ggttctgaag aacgtctaca
     3301 agcggccggc gcgccatgtc gccaagacaa tcgcctcggc cctggccaac gtccggtccc
     3361 ggaagctgga ggaatcgaac ttccggttca agacgctggt ggtggaacaa gggccgatgt
     3421 tgaagcgttc ccgggcggct tccatgggcc gggcggtttc ggtactgcac cggacttcac
     3481 acattaaagt gattctgacg gacgaatggg acaaaaagta attccgacgg cattgcggct
     3541 gggttacatt gagggctggc gctccaactg gttccagcac cggcgcctgg gtccgtccat
     3601 cctggaagac tacaaggtcc gggcttatct ccagaagcgc ttccccgaaa gcggcatcac
     3661 ccggatcgac atcgaccggc tggtcgatat gatccgcatc aagatccggg cctcgcgccc
     3721 cggcctgatc atcggccgca agggggccga gatcaataac gtcagcaagg aactgcaggc
     3781 gctctgcaac aagaatgtca ccgtggacgt cagcgaagtc accaattcct cggtggacgc
     3841 cgctttcctg gcccagagca tcgcccagca gatatcccgg cgcggcacct cctaccggtt
     3901 tgtctgcaag aatacgattg agaatgccat gcagcggggc gccaacgggg tgaagataaa
     3961 gatttccggc cggctggccg gcgccgagat cagccgttcc gaaagctaca aggacggcac
     4021 catccccctg cacacgcttt cggccatcat cgattacgcc cagactacgg cccatacgac
     4081 ctacggcgcc atcggcgtca aggtctggct gtaccggggc gacgccaagg aaaaagcctg
     4141 aattcaagga cctgaaaaat gccgttgatt cccaagcgcg taaagcatcg ccacatgcac
     4201 ggcggccgga cccggggccg ggccctgacc ggcaaccggc tttccttcgg agagtatggc
     4261 ctgctggccc tggagcccgg ctatttcaac gagcgccagc tggaagccgc ccgtgtggcc
     4321 atcacccacc acctcaaggg aaagggcaag ctctggatcc gggtcttccc ggaccggccg
     4381 atcaccggaa agccggccga gacccggatg ggcaagggca agggcgacct gaagtgctgg
     4441 acggcgcgca tcctgcccgg acgggtgatc gtggaactta ccggtgttga tctggaactg
     4501 gcccggtcgg ccttgaaact ggccgcgggc aagcttccgt tgccgacgcg ggtgatctcc
     4561 agatgaaacg aaacgatttc caggagttgc agaaactggg tccgctggag ttgcgcgagc
     4621 gggtggatag cctgaaggcc gaatactacc ggctgagatt ccagcagaag atgggtcagc
     4681 tcaaggagac ggcctccctg ggcagcgtcc gccgggacat tgcgcgggtc atgactcttc
     4741 tgaaccagaa acagaaaggt taaccccagt cggcggggat taaaagatga aaaggcaaga
     4801 atcaggcacg gttgtcagcg atcggatgga caagacccgg gtggtggtgg tggagcggct
     4861 tatccgccat ccgctttacc agcgggagat gcgccggcgc acccgttaca aggtgcacga
     4921 tcccaagaac gaaagtcata ccggcgaccg ggtcctgatc gaagagttcc ggccgatcag
     4981 ccgggacaag agctggcgat tgctgagcat catcaagaaa ggttcggaat aaaatggttc
     5041 aactgaggac aatcctggag gtcgccgaca acaccggggc caagaagatc atgtgcttcg
     5101 gcgtcctcgg cggcagccgg aagcgggtgg cccgggtcgg cgacatcatc aaggcctcgg
     5161 tcaaggacgc cctgcccaac ggcacggtca agcggcggga ggtggttcgg gcggtggtgg
     5221 tccggacgaa ggcgcatatc cgccggccgg acgggtccta tctttcattc gaccacaaca
     5281 gcgccgtgat catcgacgat gacggcaatc cgcgcggcac ccgggttttc ggtccggtgg
     5341 cgcgcgagct gcgcgataag aacttcacca agataatctc actggctccg gaagtcctat
     5401 gaaattaaga aaaggcgatt tcgttctggt tgccaagggc aaggacgccg gcaaaaaagg
     5461 ccgggtgctc cgggtttatc cggaggccaa ccgggccctg gtcgagggaa tcaacttcgt
     5521 caagcgccac ctgcgtcccg gccgggtcga acggcagggc ggcatcgttc agaaggagat
     5581 gccggtggcc attcccaacc tccgctactt ctgcctgaaa tgcaaccagg cgacccgggt
     5641 cggcttcaag ttcctcggcg attcgaaggt gcgctgctgc cggaagtgcg gcgagatcat
     5701 cgaaaccaag tccacgacgg cttgagtaaa aaggcaaaat ggcacgttta ctggataaat
     5761 acaagaaaga gatcatcgcc cggttgaccg agcagttcaa gtattccaac cagatggcgg
     5821 tgccgaagct gaccaagatc gtggtcagtg tcggcctggg caagatcggc aaagaggaga
     5881 aggtggtcga ggagatcctg ggcggcctga cggccatcac cggtcagaag ccggtaatgc
     5941 gccgggcgcg caagtcgatc gccggcttca acctgcgcac caacgatctg gtgggggcct
     6001 tcgtgaccct gcgcggccag cggatgtacg aatttctgga ccggctggtc tcctttgccc
     6061 tgccccgggt gcgcgacttc cgcggcttgc ccaagaccgg gttcgaccgg gccggcaact
     6121 acagtttcgg actcaacgaa cagaccattt tcccggagat cgactttaac aaggtgacca
     6181 aggtcttcgg aatgaacatc aacctggtga ccagcgccag gaaccggcag gaagccgaac
     6241 cgctcctgac gatgctggga ttgcccctgg aaaaagaaga gtaatcgtcc atggccaaga
     6301 agtcgatgat catcaagaac cagcacccga agtacaaggt gcgccagcga aaccgctgtt
     6361 gcatttgcgg ccggccgcgc gggtacatgc gccgtttcag cctctgccgc atctgcttcc
     6421 gagaactggc cagcaaggga ttcatcccgg gcgtggtcaa ggccagctgg taattcccca
     6481 acaagttaaa ggtgtaacgg atggtaaccg atcctatcgc tgatttgctg acgcggatca
     6541 agaacggcgg cctggctcga aagccggtca tcaggatccc gtactcggtc ttcaaggaag
     6601 acatcctcaa ggtgctggcc gaccagggat tcatcgccag ctacgcggtg gaagcggatg
     6661 gcaagaagaa gaacctgtcc gtttggctca agtaccgtcc caacagcacc cgcagcgtca
     6721 tccaggcagt ggaaatcaaa agccgcgcca gccagcggat ttacgttacc cagcaggacc
     6781 tcaagcgcct gatccggggc cgccagaaga tcggtatcct ctccacttcc cagggtgtga
     6841 tttcgcaccg ggacgccctc cggaagcggg tgggcggaga gttgctggcc gtcatctggt
     6901 aaacggcggg gtccacaacc cggaacttta acatggcaaa acaaggtaaa agaccggttc
     6961 gcattccaga cggggtcaag gcctcgctgg aggggacggt ccttaagatg agcggtccga
     7021 tgggaaacct cggcctggaa gtgcccccgg tcctgtcggt agtcctggaa aagggttcga
     7081 tccgggttga cttccggtcg gccgcgggca acggcgagcg caagaatgag acggaaaacg
     7141 aaagcctgcg cagcctctac cgggcgctca tcaacagccg gatgaaggcc gtgaaggaag
     7201 gtattaccaa gcagctggaa gtgatcgggg tcggctacca ggcggagctg aagggcaaaa
     7261 acctggtcct gaagatcggt ttttctcacc cggtctcggt accggtcccg tcggagataa
     7321 aggtcaaggt ggagaagaac ctgatcaccc tggccggttc cgatccgcag caggttggag
     7381 attttgcc
//
LOCUS       DAOQYN010000088         7362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_10474_length_7362_cov_4.853702, whole genome shotgun sequence.
ACCESSION   DAOQYN010000088 DAOQYN010000000
VERSION     DAOQYN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10474_length_7362_cov_4.853702"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PKN80_04700"
     CDS             <1..610
                     /locus_tag="PKN80_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35347.1"
                     /translation="VDQAGSGSAWSRARPLKAGADFSQARDVFNCGRLKSDRPENQSD
                     YFKIRGCYYSSAVYHFSDALMAGTCPIDLALEEIRSVLTVLGPERPLALAGRGWPALA
                     LLLLSALHPGVEWCCLDGLPASWTVQLKAGRMRLGYDYLVPGVLNHTDIPALIEANRR
                     TRYFLTGLIEPELAGVDYRDRGRFPNVVLLDRATPARLAGRL"
     gene            607..1506
                     /locus_tag="PKN80_04705"
     CDS             607..1506
                     /locus_tag="PKN80_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNQ35348.1"
                     /translation="MKFRSVSGDRAAVLAGIIACLSWGTVFVLGRVAAEADIQPVWLV
                     FLRFIFASLFLALFLTVSRTRIRLERRDLPAFLLLGATGVAGMNLFIFFALRHITATA
                     ASILENLNPVFIGLLAPVFLKERLSLRDAAGMAVGLVGCYLVVSQGRVVSFAFLGGLL
                     ALLANLCWAFYTLAARRFGVIARYGAPLATFWSAWLGVGFLGLVLLLAGLPEMPGRNG
                     WLLGAYLGVVPAGIGFTLWFFAVQRLSAVQVGVLQYLVPLTTGLIAVFWLGEVLSGWT
                     LAGGLLILAGASLPYLAGAPGPG"
     gene            complement(1476..3569)
                     /locus_tag="PKN80_04710"
     CDS             complement(1476..3569)
                     /locus_tag="PKN80_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35349.1"
                     /translation="MEETLRRQIEADLRLAGVNGGLRLGNLPEGSFEFSAGPDGSVGL
                     SAGRTRDAIAAAAWFKDRIRSGFRPEECLGRRTRCVPEFIGFTRRWRPVYKQCIDPFP
                     MTDSDIRRLAELGATGLLNGWCEGITFFFNELLPASETFPDLVSEELRRHLRAIWEVR
                     ATAARRYDLDLWRLIRAPMGTGGHVRPRELVNDPVHENFIKKHPELFGYSPVDQVSNP
                     MCLGRPEVRDFYREVYRKTAAEPGLKGFLIWLGDGNLDICRCEKCAGGPEGVLGAWAD
                     FIKFIHAAVREVNPAAAFLLIHWHCLFGQDKFRRLMKLLPPDIGVVIPKTGHRLEMSP
                     AEYEARGLRGGLNQPFLLKRGWSLETSALLEQVEAMKRPYVLFDSYAAGEDTNPAAFF
                     PAPATVAAKVIEAARKKPGPAGLFDFWGLDPDRLEVNEIALQEALWHRAGDGLREILI
                     QTARRRFALSSRRAAEGAAAAWQELDRAFQDDWPVISFGQRLENCFVGWRDATFPPDP
                     PAHEHEGFSAGPVTGELVARIPATARFQGAGRELAYFNRLEPAAFLRRSQGSAARALR
                     AAGRNLKKVLERKTRGLSIHLDFLDNAAVHFTTMARNFEALELAERVRGGRLEAAAAS
                     RRLAKLAEREIKAWEWVREFVDRYGLQNRYWPNELDRRLQLQREWASSARWLKFFREA
                     ARSRAGQPGPGAPAR"
     gene            complement(3575..4894)
                     /locus_tag="PKN80_04715"
     CDS             complement(3575..4894)
                     /locus_tag="PKN80_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNQ35350.1"
                     /translation="MNLKRRNLKETGRPMPIRTLCLVLGILSMTTLKAAADPAPPVLK
                     AGDRLALCGDSMTSVTKHSAIMTRYLYACRPELNIEVFQSAISGEDTARFLRRVPGSG
                     LETFRPTFTVIWEGHNDSRRDTDIVKAVEAYRVNLLRAVDLFQANGGRVGLASCALLG
                     PGWGKNVEENRRFVHVSDLNRRVAREKGAAFAEVTAAMNEALTKLKKTHGPDYDIVRG
                     DGGHGSWHGHMAIAIAFLRSLGLQSELARIEYEPAAGRATASTGHKVIKAGPEELVLE
                     STRYPFCFLYKHPAPDVAEMWDVAEALNFFEEFNRFTLVVKGLEPGDYEVTWGRERRT
                     FSAERLARGINLAYEFPKTPFGEAFLRVANQAYNQLRLDNIITLKRLEQNQASVAGQL
                     GLQNDTGLVQEMFREPMRVHERAVQNARAALVPVTHSIRIRRATETR"
     gene            complement(4869..6209)
                     /locus_tag="PKN80_04720"
     CDS             complement(4869..6209)
                     /locus_tag="PKN80_04720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNQ35351.1"
                     /translation="MNLKKKPGLITLLLALALAAPAAGGPPAGSAGAGRTPLLRKGDR
                     LLMTGDSITFNRKYSPIVETYLLACHPQLEITCFQSAIYGENTDRFLKRMQATALDIY
                     RPTVATTAYGMNDGRYGPYQAETGAAFLANLKAIARTFQQNGCRPILGSPGLVGPKLE
                     LEYNQTLKKFNELTRQAAAEEKCGYADIHGTMREAWEKLRKLKGDDYDLIGRDGVHAD
                     WNGQMAMATAFIRAFNLPGEIGRIEFDLKKRQATANEGHAVLETGDNTVTLESRRYPY
                     CFLWADHPTRILVEMRTVAGVLGFFEDFNRFTLVARNAEPGRYRVTWEGWSETAKRWG
                     GPSLVFDEKQLKAGVNLAREFDRNPFTNKFLELQSAVATRQEVQNLVPLKQPVPNHLL
                     NLCLQEEPGLDTALLVKDPVRVQEILYRRILKIRTPIRHRLRMEKIDEPQAAQP"
     gene            complement(6220..6996)
                     /locus_tag="PKN80_04725"
     CDS             complement(6220..6996)
                     /locus_tag="PKN80_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35352.1"
                     /translation="MKMRKGWSTLIAAALLMTAAAGLYADDTPVGIYFNPVRGDDSSH
                     CYGYEGTRIALEENGFDNVSYVKSLKAEDLKGIKVLIMSCVLGYPRGWDLKQVKEEQR
                     RFVENGGGLVLIQESIGWRGIFKDNPIFPEFGRGVAHDGKSYGPVQLTPLLVIDKKHP
                     ITRDLPDQFPMQYDAAPLKPGEKGRVLIMLSDDAIVRSVKTKIDKMAALIVGEHGKGR
                     VVLIGPLVGVSRLPREMENPPAGGELKMLLNSVRWAAGGN"
     gene            complement(7021..>7362)
                     /locus_tag="PKN80_04730"
     CDS             complement(7021..>7362)
                     /locus_tag="PKN80_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35353.1"
                     /translation="AAAARISRSGLAAVADLGKGKVVLLQVAADRFKEAEDFWGRSKI
                     IRLYATVLNNLGGRSRVEPDLAAIGGWGLVNEWLPGYADRVPKRAPKVQESNLYQQPT
                     LDWDPDAHVAF"
BASE COUNT         1051 a         2444 c         2478 g         1389 t
ORIGIN      
        1 tgttgaccag gccggaagtg gttcggcctg gtcccgcgcc cggccgctca aggccggcgc
       61 cgacttcagc caggcgcggg acgtcttcaa ctgcggccgc ctgaagagcg accggcccga
      121 gaaccagagc gattacttca agatccgcgg ctgctactac tcttcggccg tctatcattt
      181 cagcgacgcc ctgatggccg gcacctgccc gattgacctg gccctggagg agatcagaag
      241 cgtgttgacc gtcctgggac cggagcggcc gctggccctg gcggggcggg gttggccggc
      301 tctggcgctg ctcctgttga gcgcgctcca tcccggcgtg gaatggtgct gcctggacgg
      361 ccttccggcc agctggacgg tccagttgaa ggccggccgg atgcggctcg gatatgacta
      421 cctggtgcct ggagtcctca accataccga cattccggcc ctcatcgagg ccaaccgccg
      481 gactcgttac ttcctgaccg gcctgatcga gccggagctg gcgggagttg attaccggga
      541 ccgcggccgt tttcccaacg tcgtcctgct ggaccgggcg accccggccc gcctggccgg
      601 ccgcctgtga agtttcgatc ggtctccggc gaccgggcgg cggttctggc cgggatcatc
      661 gcctgtctca gctggggcac ggtcttcgtg ctggggcggg tggcggccga ggcggacatc
      721 cagccggtct ggctggtctt cctgcgcttc atctttgcct cgttattcct ggcccttttt
      781 ctgaccgttt cccggacccg gatccggctc gagcggcgcg acctgccggc cttcctgctg
      841 ctcggggcca ccggcgtcgc cggcatgaac ctcttcatct tcttcgccct gcgccacatc
      901 accgccaccg ccgccagcat cctggagaat ctcaacccgg tcttcatcgg cctgctggcc
      961 ccggtctttc tgaaggagcg cctctccctg cgggatgcgg ccggaatggc cgtcggcctg
     1021 gtcggctgct acctggtggt cagccagggt agagtggttt ccttcgcctt cctgggcggc
     1081 ctgctggccc tgctggccaa cctctgctgg gccttttaca cgctggccgc ccggcggttc
     1141 ggggtgatcg cgcgctacgg ggcgccgctg gccaccttct ggtcggcctg gctgggggtg
     1201 ggtttcctgg ggctggtcct gcttctggcg ggcctgccgg aaatgcccgg ccgcaacggc
     1261 tggctgctgg gcgcctacct gggcgtggtt ccggccggca tcggtttcac gctctggttc
     1321 tttgccgtcc agcgcctgag cgcggtccag gtcggtgttc tgcaatacct ggtgccgctg
     1381 accaccggcc tgatcgccgt cttctggctg ggcgaagttc tctccggctg gaccctggcc
     1441 ggagggctgc tgatcctggc cggcgcctcc ctgccttacc tggccggggc tcccggtccg
     1501 ggttgaccgg cccggctccg ggccgcttcc ctgaagaact tcaaccagcg ggccgacgag
     1561 gcccattccc gctgcagctg caggcgccgg tccagctcgt tcggccagta acggttttga
     1621 agcccgtacc ggtcgacgaa ctcccggacc cattcccagg ccttgatttc ccgctcggcc
     1681 agcttcgcca gccgccggct ggccgcggcc gcctccagcc ggccgccgcg cacccgttcc
     1741 gccagttcca gggcctcgaa attccgggcc atggtggtga agtgcacggc ggcattatcc
     1801 aggaagtcga ggtggatgga gaggccgcgg gttttccgtt ccaggacctt cttcaggttc
     1861 cggccggcgg cccggagggc ccgggctgcg cttccctggc ttcggcgcag gaaggcggcc
     1921 ggctccaggc ggttaaagta agccagttcc ctgcccgcgc cctggaaacg ggcggtcgcc
     1981 gggatgcggg ccaccagctc gccggtcacc gggccggctg agaagccctc gtgttcatgg
     2041 gcgggcgggt caggcgggaa ggtggcgtcc cgccagccga cgaagcaatt ctccagccgc
     2101 tggccgaagg agatcaccgg ccagtcatcc tggaaggccc ggtccagttc ctgccaggcc
     2161 gcggcggcgc cttcggccgc ccggcggctg gaaagggcga atcggcgccg ggccgtctga
     2221 atcaggatct cccgcaaacc gtcacccgcc cggtgccaga gcgcctcctg gagggcgatc
     2281 tcgttgacct ccagccggtc cgggtccagt ccccagaaat cgaagaggcc ggccgggccg
     2341 ggtttcttcc gggcggcctc gatgaccttg gcggcgaccg tggccggcgc cgggaagaag
     2401 gcggccgggt tggtgtcctc gccggcggcg taggagtcga agaggacata ggggcgtttc
     2461 atggcctcga cctgctcgag gagggcgctg gtctccagcg accagccgcg tttcagcagg
     2521 aagggctggt tcaggccgcc cctcagtccg cgcgcctcgt attcggccgg gctcatttcc
     2581 agccggtggc cggtcttggg aatgaccacg ccgatatccg gcggcagcag tttcatgagg
     2641 cgccggaact tgtcctggcc gaagaggcag tgccagtgga tgagcaggaa agcggcggcc
     2701 gggttgactt cgcggaccgc ggcgtgaatg aatttgatga agtcggccca ggcccccagg
     2761 acaccctccg gtccgccggc gcacttttcg cagcggcaga tgtccaggtt gccgtcgccc
     2821 agccagatca gaaaaccctt cagcccgggt tcggcggccg tcttccggta gacctcccgg
     2881 taaaaatcgc gcacctccgg ccggcccagg cacatcgggt tggaaacctg gtcgaccggc
     2941 gaataaccga agagttccgg gtgctttttg atgaaatttt cgtggaccgg gtcgttgacc
     3001 agctcgcgcg gccgcacgtg gccgccggtg cccatgggcg cccggatcag ccgccagagg
     3061 tccaggtcgt aacggcgggc ggccgtcgct cgtacctccc agatcgcccg caggtgccgg
     3121 cgcagctctt cggagaccag gtccggaaag gtttcggagg ccggcaggag ttcgttgaag
     3181 aagaaggtga tgccttcgca ccagccgttc aggaggccgg tggcgccgag ttcggccagg
     3241 cgccggatgt cggaatcggt catcgggaag gggtcgatgc actgcttgta aaccggccgc
     3301 cagcgccggg tgaagccgat gaactccggc acgcagcggg tgcggcggcc caggcactcc
     3361 tccggccgga aaccgctgcg gatccggtcc ttgaaccagg cggcggcggc gatcgcgtcg
     3421 cgggtccgtc cggcgctcag gccgacgctt ccgtccggac cggcgctgaa ttcgaagctt
     3481 ccttccggaa gatttccaag ccggagaccc ccgttgacgc cggccagccg caggtcggcc
     3541 tcgatctggc ggcgcagtgt ctcttccatt tttcttatct ggtttcagtc gcccggcgga
     3601 tccgaatcga atgggttacc ggcaccaggg cggcccgggc gttctggacg gcccgttcgt
     3661 gcacccgcat cggctcccgg aacatctcct ggaccagacc ggtgtcgttt tgcaggccca
     3721 gctggccggc cacgctggcc tggttctgct ccaaccgctt gagggtgatg atgttatcca
     3781 gtcgcagctg gttgtaagcc tggttggcga cccgcaggaa ggcctccccg aagggcgtct
     3841 tgggaaactc gtaggcgagg ttgatgcccc gggccaggcg ttcggccgaa aaggtccggc
     3901 gttcccgtcc ccaggtcacc tcgtaatcac ccggctccaa ccccttgacc accagcgtga
     3961 accggttgaa ctcctcgaag aaattcagcg cctcggccac gtcccacatt tcggcgacgt
     4021 ccggcgccgg atgcttgtag aggaagcaga aggggtagcg ggtgctctcg aggaccagtt
     4081 cctcgggacc ggccttgatg accttgtgcc cggtgctggc ggtggcgcgg ccggcggccg
     4141 gttcgtactc gatccgggcc agttccgact ggaggccgag gctgcgcagg aaggcgatgg
     4201 cgatggccat gtgtccatgc caggagccat ggccgccgtc gccccggacg atatcataat
     4261 ccggcccgtg cgtcttttta agctttgtca gcgcctcgtt catggccgcg gtcacctcag
     4321 cgaaggcggc ccccttctcc ctggccaccc gccggttgag gtcggaaaca tggacgaaac
     4381 ggcggttctc ctcgacgttt tttccccagc ccgggcccag tagggcgcag gaggccaggc
     4441 caacccggcc gccgttggcc tggaagaggt ccaccgcccg gagcaggttg acccggtagg
     4501 cctcgacggc cttcacgatg tcggtatcgc gccggctgtc gttatgcccc tcccagatga
     4561 ccgtgaaggt cggccggaag gtttccagcc cgctgcccgg cacgcggcgc aggaagcggg
     4621 cggtatcctc gccggagatg gcgctctgga agacctcgat gttcaattcc ggccgacagg
     4681 cgtagagata gcgcgtcatg atggccgagt gcttggtgac cgaggtcatc gagtcgccgc
     4741 agagggcgag ccggtcgccg gccttcagga ccggcggggc cggatcggcg gcggccttca
     4801 aggttgtcat cgaaaggatt cccaggacga gacagagtgt ccggatcggc atcggccggc
     4861 cggtttcctt aaggttgcgc cgcttgaggt tcatcgatct tctccatccg cagtcggtgc
     4921 cggatcgggg tccggatctt caggatacgc cggtaaagga tctcctggac ccggaccgga
     4981 tccttgacca gcagggcggt gtccaggccc ggctcctcct gcagacagag gttcagcagg
     5041 tggttgggaa ccggctgttt cagcggaacc aggttctgga cctcctgccg ggtcgcgacc
     5101 gccgattgca attccaggaa cttgttggtg aagggattcc ggtcgaattc ccgggccagg
     5161 ttgacgcccg ccttcagttg cttctcgtcg aagaccaggc tgggaccgcc ccagcgtttg
     5221 gccgtttcgc tccagccctc ccaggttacc cggtaacggc cgggttcggc attcctggca
     5281 accagggtga aacggttgaa atcctcgaaa aagccgagga ccccggccac cgtccgcatc
     5341 tccaccagga tccgggtcgg gtggtcggcc cagaggaagc agtaagggta gcgccggctt
     5401 tcgagcgtga ctgtgttatc cccggtttcc agcaccgcgt gtccttcgtt ggccgtcgcc
     5461 tgccgttttt tgaggtcgaa ctcgatccgg ccgatttcgc cgggcaggtt gaaggcccgg
     5521 atgaaggccg tcgccatggc catctggccg ttccagtcgg cgtggacgcc gtcccggccg
     5581 atcaggtcgt aatcgtctcc cttcagtttt cggagctttt cccaggcctc gcgcatcgtg
     5641 ccgtggatgt cggcgtagcc gcacttctcc tcggccgccg cctggcgggt cagttcgttg
     5701 aattttttga gggtctggtt gtactccagt tccagcttgg gcccgaccag cccgggcgag
     5761 cccaggatcg gccggcagcc gttctgctgg aaggttcggg cgatggcctt caggttggcc
     5821 aggaaggcgg cgccggtctc cgcctggtag ggtccgtagc ggccatcgtt catcccgtag
     5881 gcggtggtgg ccaccgtcgg ccggtaaatg tccagggcgg tcgcctgcat ccgcttcagg
     5941 aagcggtcgg tattctcgcc gtagatggcg ctctggaaac aggttatttc cagctgcgga
     6001 tggcaggcca ggagataggt ttcgacgatc ggcgagtact tccggttgaa ggtgatcgaa
     6061 tcgccggtca tcagcagtcg gtcgcccttc ctgagcagcg gcgtccgtcc ggcgccggcg
     6121 ctgccggccg gcggaccgcc ggcggccggc gccgccagag ccagggccag cagcagcgtt
     6181 atcaggcccg gctttttttt aaggttcatc ggccgttcct taattgccgc cggccgccca
     6241 gcggaccgag ttgagcagca tcttcagctc gccgccggcg ggcgggtttt ccatctcgcg
     6301 gggcaggcgg ctcacgccca ccagcggtcc gatcaggacc acccggccct tcccgtgttc
     6361 gccgacgatc agggcggcca tcttgtcgat tttggtcttt acggaacgga cgatggcgtc
     6421 gtccgagagc atgatcaata cccggccctt ctcgcccggt ttcaatgggg cggcatcgta
     6481 ctgcatcggg aactgatccg gcaggtcgcg ggtgatgggg tgctttttgt cgatcacgag
     6541 cagcggcgtc agctggaccg ggccgtaact cttgccgtcg tgggccacgc cccggccgaa
     6601 ctccgggaag atcgggttat ccttgaagat gccgcgccag ccgatcgact cctggatcag
     6661 taccaggccg ccgccgttct ccacgaaccg gcgctgttcc tccttcacct gtttcaggtc
     6721 ccagcccctg gggtagccga gcacgcagga catgatcagc accttgatgc ccttcaggtc
     6781 ctcggccttg agcgatttca cgtaactcac gttgtcgaag ccgttctcct cgagggcgat
     6841 gcgcgtcccc tcgtatccgt agcaatggga ggaatcgtcg ccccggaccg ggttgaagta
     6901 gatgccgacc ggcgtgtcgt cggcgtagag gccggcggcg gccgtcatca gcagggccgc
     6961 cgcgatcagg gtgctccatc ctttccgcat tttcatctcc gcctcctgag gtcggttcgg
     7021 ttaaaaggcc acgtgggcgt ccggatccca gtccagggtc ggttgctggt aaaggttgct
     7081 ctcctggact tttggggccc gcttgggcac ccggtccgcg tacccgggca gccattcgtt
     7141 gaccaggccc cagccgccga tggccgccag gtcgggttca acccggctcc ggccgcccag
     7201 gttgttcagg acggtcgcgt agaggcggat gatcttggag cggccccaga aatcctccgc
     7261 ctccttgaac cggtcggccg ccacctggag caggacgact tttcccttgc ccaggtcggc
     7321 caccgccgcg aggcccgacc tggagatccg ggccgcggcc gc
//
LOCUS       DAOQYN010000089         7308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_10584_length_7308_cov_3.892045, whole genome shotgun sequence.
ACCESSION   DAOQYN010000089 DAOQYN010000000
VERSION     DAOQYN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10584_length_7308_cov_3.892045"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKN80_04735"
     CDS             <1..227
                     /locus_tag="PKN80_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNQ35354.1"
                     /translation="RWPGRTAALVGVKNRVLSARLLATGRPVPVIEQPGAGRLILKGL
                     PARPPDRHDTVIALELDGPPEAVPYDGEPL"
     gene            complement(256..1338)
                     /locus_tag="PKN80_04740"
     CDS             complement(256..1338)
                     /locus_tag="PKN80_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35355.1"
                     /translation="MFKEVRRHAITEYRTESRTSADSWADEAYRREYHAFTSLVWSPK
                     RKKLYCGLTNFGNDLLWEFDPAGGTFKDLGYRQAGFCEPFDIKLHRSLEIDSRGVLYG
                     ATSCLHGIEKRAEGPGGKVFSYDPETGQYRLLAIPKSRDYIQTISLDEKRAMIYGFTY
                     PVFEFFAYSIGENRVRYCEYQESIVHVGAVDDRGRYWGTWSSRHHLFSYDPAENRVTY
                     HQHGFPETFHSLMYPNAGPVDAMINGGDGFIYAATENATLYRLDPESARLEYLGKPYP
                     GNRMPGLQVAADGLIYGCGGSDNQPVVFRYDRRSGRFETLGEIRTSAGGEVCFRTHDL
                     EVVGNTIYVGETDNPSRTNYLWECRI"
     gene            1537..2424
                     /locus_tag="PKN80_04745"
     CDS             1537..2424
                     /locus_tag="PKN80_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNQ35356.1"
                     /translation="MDGNIFRSREDPQRKQEEQNARLLAAAYEGRVDRVQEAIKSGAD
                     VNARNRAGDTALMLAVIKNHDNVVHLLLEIGADINARNGKGLNALGLSEWCKHEVISG
                     MLRAAGATAEGDATVIPDFVEKTLQGGLQDIDWDKFSRKSAPSELPAEAPGRLGSLLG
                     RFGGRRRQLNDDLLAAARSGADMEIRRLVEAGARVDITDSQNGATPLMYAAAGGHRET
                     ARLLIMLGAPLNSRDNAGRTALIWAVTAGHLELVKLLVNYRADSTIKDNLGNTPLKWA
                     HSFGHQEILSFLLNSGVTR"
     gene            2421..3542
                     /locus_tag="PKN80_04750"
     CDS             2421..3542
                     /locus_tag="PKN80_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNQ35357.1"
                     /translation="MNPKPASPGRAAVFTGPGRPLEIRLYPVARPAPGRTLLKLERSG
                     ICGTDLHIRAGRLPVPTGGLIIGHEFVGRVIELGPGRRLDGLGERLKVGDLAIACVAL
                     PCGRCFNCRRGETASCLNFGVTYLADPGQAPHFHGGFAEYLFSPTANLVKVPAGLDPD
                     AVAAFACAGPTVIRAFDFAGGARAGELVVVQGSGPVGLFAAAWAARAGGRVVLIGSSS
                     HPKRLALARELGAVLTIDYRRTSEADRLQKILRLAEKMKRGNGADLVFEASGSPAAVP
                     EGLNLVRTLGRYVIPGQYSASGGVEIQPQLITFKAIRITGSGQYKLADIRAYLDFLRK
                     HPDLQPLLARSVRRYRIETAERALEDVAAGRVIKGVFSS"
     gene            3634..>7308
                     /locus_tag="PKN80_04755"
     CDS             3634..>7308
                     /locus_tag="PKN80_04755"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNQ35358.1"
                     /translation="MSRRLCLSIVLILLAVSSAHGADRRVIEAEEFDGLVRYPYHKED
                     ARGWYARESTCRSYGAVGRGYHAQIHENAVDPVASKRLARPLPAGKYRVFLRVAGNNW
                     QDRDNIVEVGLGEAAARFEWRGRGRFDWRPFREVELSRPVAEITLKAVQFGGKGFRHL
                     YEGNLRTLAIDTIYITSDLSEAAGPSAEGTSLIELGREAPLEVLSGQAKTSRRTVLQA
                     ETPLPLDQPRVTPVKLAALDGRRNLWPNSSFELGGADGWGGVSDNYLSLHIFDERDHR
                     EGNAFHGRYSQRIPAGAHGTSRLVYLAEAGTYTLSLYIRGEANSTATVRLAATRTDRN
                     GREQFVDEKNQPPAGLTIPVRLSGEWQRVNASGPLAAGGFVLRVQTSRECFVDAVQLE
                     AGGEATPYQPRAAIEAGLATAELGNILYDDRPVLVAWATNSGNAAGTAVLAYDIVDVR
                     DRRVAAGTVEIPVTAGATVRREVPLTPVRRGLFSCFYGLQGRPAPEGELVYAVLPKPP
                     AAAPRHALACNMDNLPAALSLMKRMGHRWQLYCKTSRATSPASINPAPDRWVWDPAHE
                     VDRLPTSLGLENMPCLWPAQLPKHLADAALSDTKSVGSGRRDVVRRSETGGLPMMPDL
                     VKWRQYCAEVGRNLPEINWWTIDDETEMYFSPREFARIVDATCQGFADAGSKAGISQS
                     CTPDYTEDMLAELGGQPPFQGFGASSYSFEYWDAVKVRGLQRRYGLPWSCIGVGWDNQ
                     PQMFHSLPGYTPVYASAARTARELVFLTLVQDARVIGHYTGRLWMRTGLFNTDFPLMD
                     WNGVPLPHGFSYSCVGLLLANAVPQEDIYLEPLDTLLFVYRQDGRLGAATWANNTPNL
                     DIHWKTWPRTVEGFTIPAEVDVLDMYGNPLEGAVVGNGKTVVTLTEEPFFFLNRNLSE
                     ADFLAALRSASAPPPPVEMRLAFLPDGRGGVDLGVWTANHTGRPLTGLKLDANFPGDR
                     MVTKTDWMLPDRNGKLPDLPAGQAAFGRIPTAIGLDYPVENATFSVWLSDGQAEYPWY
                     ERCWLTVAPRVTATGGRVTDWSKVQPAWLYHTFSWGRFGRAFPQVVSGAENLKYAFRV
                     DGRAAVRTGYDGRNLYVSIEVEDDNAVFTGAPEKRDRFELKFDRDLAGDFGSASARPD
                     LNLLVLPESSGPCQVKGVEGAAAVLTRRSAGGYTVEVSVPLGAITLRPAAAGGAIGFD
                     LVWTDADPETGGVASSAMRWA"
BASE COUNT         1162 a         2418 c         2448 g         1280 t
ORIGIN      
        1 tccgctggcc gggccggacc gcggcgctgg tcggggtgaa gaaccgggtc ctctcggccc
       61 ggctgctggc caccggccgg ccggtgccgg tgatcgagca gcccggcgcg ggtcggctga
      121 tcctgaaggg tctgccggcg cggccgccgg accggcatga cacggtcatc gccctggaac
      181 tggacggacc gcccgaggcg gtgccctacg atggagaacc gctttgatcc cggggtcttc
      241 agccccggcc gcggttcaga tccggcattc ccagaggtag ttggtccggc tcgggttatc
      301 cgtctcgccg acgtagatgg tattacccac cacttccagg tcgtgggtcc ggaagcagac
      361 ctcgccgccg gccgaagtcc tgatttcgcc cagcgtttcg aagcggccgg accgccggtc
      421 gtaacgaaag acgaccggct gattatcgct tcctccgcag ccgtaaatca ggccgtcggc
      481 ggccacctgc aggccgggca tccgattgcc cgggtagggc ttgcccaggt actccaggcg
      541 ggcgctttcc gggtcgagcc ggtagagggt ggcgttttcg gtggccgcgt agatgaagcc
      601 gtcgccgccg ttgatcatgg cgtcgaccgg gccggcgttc gggtacatca ggctgtgaaa
      661 ggtctccggg aaaccgtgct ggtgataggt tacccggttt tcggccgggt cgtaactgaa
      721 gagatggtga cggctgctcc aggtgcccca gtagcggccg cggtcgtcca ccgccccgac
      781 gtggacgatg ctctcctggt actcgcagta acggacccgg ttctccccga tcgagtaggc
      841 gaagaattcg aaaaccgggt aggtaaagcc gtagatcatg gcccgttttt cgtccaggct
      901 gatggtctgg atgtagtcgc gcgacttggg aatggccagg agccggtact ggccggtctc
      961 gggatcgtaa ctgaagacct tgccgcccgg cccttcggcg cgcttttcga tgccgtgaag
     1021 gcagctggtg gccccgtaga ggacgccccg ggaatcgatc tccagggagc ggtgaagctt
     1081 tatgtcgaag ggttcgcaga agccggcctg ccggtacccg aggtccttga aggtgccgcc
     1141 ggccgggtcg aattcccaga gcagatcatt tccgaagttg gtcagaccgc agtacaattt
     1201 tttgcgcttc gggctccaga ccagcgaggt gaaggcgtga tattcacgcc ggtaggcctc
     1261 gtccgcccag gagtcggccg aagttctcga ttcggtacgg tattcggtga tcgcgtggcg
     1321 cctgacttcc ttgaacattt tacctccttg ccggaaacgg ttgaaaattg ccggtaaaca
     1381 agcgtcaaac aaaattatac cacgaaaagt tttggttcga tcaggccgct tttttgcctt
     1441 gaactacccg tgatcgtatg gtattatcag aatgttttag gtgtttcttc tttaaatttt
     1501 tattattgca aaatccgcct ttgaaaaagg agaagcgtgg acggcaatat ttttcggtcg
     1561 cgggaagacc cccaacggaa gcaggaagag cagaatgccc ggttgctggc cgcggcctac
     1621 gaaggccggg ttgaccgggt ccaggaggcg atcaagtccg gcgccgacgt caatgcccgg
     1681 aaccgggccg gcgataccgc cctgatgctg gcggtcatca agaatcatga caacgtcgtg
     1741 cacctgctgc tggagatcgg ggccgatatc aacgcccgca acggcaaggg gttgaacgcc
     1801 ctcggattgt cggagtggtg caagcacgag gtgatttccg ggatgcttcg ggcggccggc
     1861 gcgacggccg agggagacgc gaccgtcata cccgatttcg tggaaaagac gctccagggc
     1921 ggactgcagg atattgattg ggacaagttc tcccggaagt cggccccgtc cgagttgccg
     1981 gccgaagcgc ccggccggct cggcagcctg ctgggccgtt tcggaggccg gcggcgccag
     2041 ttgaacgatg acctgctggc ggccgcccgt tccggcgcgg acatggagat ccggcggctg
     2101 gtcgaggccg gcgcccgggt ggacataacc gacagccaga acggggctac gcccctgatg
     2161 tatgccgccg ccggcggcca ccgtgaaaca gcgcgtctcc tgatcatgct gggcgccccg
     2221 ctcaacagcc gggataacgc cggccggacc gccttgatct gggccgtgac cgccggccac
     2281 ctggaactgg tcaagctgct ggtgaattac cgggccgatt caaccatcaa ggacaacctg
     2341 ggcaatacgc cgctcaagtg ggctcactcc ttcggccacc aggagatcct ttccttcctg
     2401 ctcaacagcg gggtcacccg gtgaatccga aaccggcgtc tccggggcgg gcggcggtct
     2461 tcaccggccc gggccgcccc cttgagatcc gcctttatcc cgtggcccgg ccggccccgg
     2521 ggcggaccct tttgaagctt gaacgcagcg gcatctgcgg caccgatctg cacatccggg
     2581 ccggccggct tccggtgccg acgggcggcc tgatcatcgg ccacgaattt gtcggccggg
     2641 tgatcgagct ggggccgggc cgccggcttg acggcctggg agaacgcctc aaggtcgggg
     2701 acctggccat cgcctgcgtg gctttgccct gcggccgctg tttcaattgc cgccggggcg
     2761 aaacggccag ctgcctgaat tttggagtga cctacctggc cgatccgggc caggcccccc
     2821 atttccacgg cggtttcgcc gagtaccttt tcagcccgac cgccaacctg gtcaaggttc
     2881 cggccggcct ggacccggat gcggtggccg ccttcgcctg cgccgggccg accgtgatcc
     2941 gggcctttga tttcgccggc ggcgccaggg ccggggaact ggtggtggtc cagggaagcg
     3001 gtccggtcgg actcttcgcg gccgcttggg cggcccgggc cggcggccgg gtggtcctga
     3061 tcgggtcgag ttcccatccg aaacggctgg cgttggcccg tgagctcggg gcggtcctga
     3121 ccatcgatta ccgccggacg agtgaagccg accggttgca aaaaatcctc cggctggccg
     3181 aaaaaatgaa gcgcggcaac ggcgccgacc tggtcttcga ggccagcggt tccccggccg
     3241 ccgttcccga ggggctcaac ctggtccgga cgctcggccg ctacgtcatc cccggccagt
     3301 attcggccag cggcggcgtg gagatccagc ctcagctgat caccttcaag gcgatcagga
     3361 taaccggcag cggtcagtac aagctggctg atatccgcgc ctacctggac tttctgcgga
     3421 agcacccgga cctccagcct ctcctggccc ggagcgtgcg ccggtaccgg atcgagacgg
     3481 ccgagcgggc cctggaggat gttgccgccg gccgggtcat caagggcgtt ttttcctcgt
     3541 gagattttct tctttttaac gccgggcgcc ttgacccatc ggagggcgtc caagcgaagt
     3601 tccgcgtaac ctggttctga caagggggac atgatgtcaa gacgcctgtg cctgtcgatc
     3661 gtcctgattc tgcttgccgt ttcttccgcg cacggcgccg atcgccgggt gatcgaggcc
     3721 gaggaatttg acggcctggt ccgttatccc taccacaagg aggatgcccg cggctggtac
     3781 gcccgcgaga gcacctgtcg ttcctacggc gcggtcggcc gcggttacca cgcccagatt
     3841 cacgagaacg cggtcgatcc ggtcgcttcg aagcggctgg cccggccgct tccggccgga
     3901 aagtaccggg tcttcctgcg cgtcgccggc aacaactggc aggatcggga caacatcgtc
     3961 gaggtcggat tgggagaagc ggcggcccgt ttcgaatggc gggggcgggg ccggttcgac
     4021 tggcgtcctt tccgggaagt tgagctgagc cggccggttg cggagataac cttgaaggcg
     4081 gtccagttcg gcggcaaggg tttccgccat ctctacgagg gcaatctccg cacgctggcg
     4141 attgacacca tctacatcac ctcggatctt tccgaggcgg ccggtccgag cgccgaaggc
     4201 acctcgctga tcgaactggg ccgggaggct ccgctggagg tgctctccgg ccaggcgaag
     4261 acgtcccgcc ggaccgttct ccaggctgag acgccgctgc ctctcgatca gccgcgggtc
     4321 accccggtca agctggccgc cttggacggc cgccggaacc tctggccgaa ctcctccttc
     4381 gagctcggcg gcgccgacgg ctggggcggt gtcagcgaca actatctcag cctccacatc
     4441 tttgacgagc gggaccaccg ggaggggaac gccttccacg gccgttactc ccagcgtatc
     4501 ccggccggcg cgcacggcac cagccggctg gtctacctgg ccgaggccgg tacctacacc
     4561 ctttcccttt acattcgcgg ggaggccaac tccacggcca cggttcggct ggccgccacc
     4621 cggaccgacc gcaacggccg ggagcagttc gtcgacgaaa agaaccagcc gccggccggc
     4681 ctgacgattc cggtccggct gagcggcgaa tggcagcggg tgaacgccag cggccccctg
     4741 gcggcgggcg gtttcgtcct gcgcgtccag acgtcccggg agtgtttcgt tgacgcggtg
     4801 cagctggagg ccggcggcga ggcgactccc taccagccgc gggcggcgat cgaagccggc
     4861 ctggccaccg cggaactggg caacatcctt tacgacgacc ggccggtcct ggtcgcctgg
     4921 gctaccaaca gcggcaacgc ggccgggacg gccgtcctgg cttacgatat tgttgatgtg
     4981 cgcgaccggc gcgtggccgc cggcacggtc gaaatcccgg tgacggccgg cgccaccgtg
     5041 cgccgcgagg tgccgctgac cccggtgcgc cgcggcctct tcagctgctt ctacggcctc
     5101 caggggcggc cggcgccgga aggcgaactg gtctacgcgg ttctaccgaa gccgccggcg
     5161 gcggcgccgc gccacgccct ggcctgcaac atggacaacc tgccggcggc cctgtccctg
     5221 atgaaacgca tgggccaccg ttggcagtta tactgcaaga ccagccgggc tacttctcct
     5281 gcctcgatta atccggcccc ggaccgctgg gtctgggatc cggcgcacga ggtcgaccga
     5341 ttgccgacct cgctgggcct ggagaatatg ccctgcctct ggccggccca actgcccaaa
     5401 cacctggccg acgcggccct ttccgacacg aaatcggtcg gcagcggccg gcgcgacgtg
     5461 gtccggcgct ccgagaccgg cggcctgccc atgatgcccg acctggtcaa gtggcgccag
     5521 tactgcgccg aagtgggccg caacctgccg gagatcaact ggtggaccat cgacgacgag
     5581 acggagatgt acttcagtcc gcgcgaattc gcccggatcg tggatgccac ctgccaggga
     5641 ttcgccgacg ccggttccaa ggccggcatc agccagagct gcaccccgga ttacaccgag
     5701 gatatgctgg ccgaactggg cggtcagccg cccttccagg gattcggcgc ctccagctac
     5761 agcttcgagt actgggacgc ggtcaaggtg cgcggactcc agcgccggta cggcctgccc
     5821 tggagctgca tcggcgtcgg ctgggataac cagccgcaga tgttccactc cctgcccggc
     5881 tacacgccgg tctacgcctc ggcggcccgg accgcccggg agctggtctt cctgaccctg
     5941 gtccaggacg cccgggtcat cggccactac accggccgcc tctggatgcg gaccggcctc
     6001 ttcaataccg attttcccct gatggattgg aacggcgtgc cgctccccca cggcttcagc
     6061 tacagttgcg tcggacttct cctggccaat gccgtcccgc aggaggatat ttacctggag
     6121 ccgctggaca cgctgctctt cgtttaccgc caggacggcc ggctgggcgc ggcgacctgg
     6181 gcgaacaaca cgccgaacct tgatatccac tggaagacct ggccgcggac cgtcgaaggg
     6241 ttcacgattc cggccgaggt tgacgtgctg gacatgtacg gcaatccgct ggaaggtgcg
     6301 gtggtgggca acggcaagac cgtggtgacc ctgaccgagg agcccttctt ctttttgaac
     6361 cgcaaccttt ccgaggccga tttcctggcc gcgctccgct cggccagcgc gccgccgccg
     6421 ccggtcgaga tgcgcctggc cttccttccc gacggccggg gcggggtgga cctgggcgtc
     6481 tggacggcca accataccgg ccggccgctg accggtctga aactcgacgc caacttcccg
     6541 ggcgaccgga tggtgaccaa gaccgactgg atgcttcccg accgcaacgg caagctgccg
     6601 gacctgccgg ccggccaggc ggcctttggc cgcatcccga cggccatcgg cctcgattac
     6661 ccggtggaga acgccacctt cagcgtctgg ctcagcgacg gccaggccga gtatccctgg
     6721 tacgaacgct gctggctgac ggtcgccccg cgggtcacgg cgaccggcgg ccgggtgacc
     6781 gactggtcga aggttcagcc ggcctggctg taccatacct tcagctgggg ccgcttcggc
     6841 cgcgccttcc cgcaggtggt ttcgggcgcc gagaacctta agtatgcctt ccgggtggac
     6901 ggccgggccg cggtccggac cggctacgac ggccgcaacc tctatgtttc gatcgaagtc
     6961 gaggacgata acgccgtttt caccggcgcc ccggagaaac gcgatcgttt cgaactgaag
     7021 tttgaccggg acctggccgg cgatttcgga tccgcttcgg cccgaccgga ccttaacctg
     7081 ctggtgcttc cggagagttc cggtccctgc caggtcaagg gtgtcgaggg ggccgccgcc
     7141 gtcttgaccc ggcggtcggc cggcggatac acggtggagg tttccgtgcc gctgggcgcg
     7201 atcacgcttc ggccggccgc cgccggcggg gcgatcggct tcgacctggt ctggacggac
     7261 gccgacccgg aaaccggcgg agtcgcctcc agcgccatgc gctgggcc
//
LOCUS       DAOQYN010000090         7294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_10608_length_7294_cov_4.711701, whole genome shotgun sequence.
ACCESSION   DAOQYN010000090 DAOQYN010000000
VERSION     DAOQYN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10608_length_7294_cov_4.711701"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PKN80_04760"
     CDS             <1..826
                     /locus_tag="PKN80_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35359.1"
                     /translation="RQAAAGAFGDFVYAEGEYCHDIDAGCNLRQVAAMRAASAAGQEW
                     KQVSRKYQSAGIETGPMHYPTHSTSGPVSVMKAHAVKVTAYGYRNRDGKGCAQADEIT
                     NEVALFRMSNGAAVRIAELREAPGILGQDSETFRIMGTRGSFSENRWFEIERPDPARL
                     DLNNLPKPRVTKLTAAEMFDPLPPEVQEAFKRAMYRNEPIDEKDLQTRDFVPAGHGGS
                     HPYLVHEFVEAVTAGRQPAINIWEAARYMVMGVMAHKSALKEGVTLDVPDWGDAPA"
     gene            complement(857..2398)
                     /locus_tag="PKN80_04765"
     CDS             complement(857..2398)
                     /locus_tag="PKN80_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35360.1"
                     /translation="MNELKIGWAARDISTEKPINIPGQFHMRISEGVMDPLTVSALVI
                     DNGSDLVIFLSADLVVIRSGLLDEIRDKVAESNPEIPVAKILMNATHAHTGASHYADS
                     IWSTSSNSLANMYAETPHDGIEIASSDEYRHFLAGQAAEAVCEAYAKRGAGGIAYGYG
                     YAVVGHSRRVVYFDDTSKRPGAESNSRSGARGHAVMYGNTNDPNFSHYEAGADHFINL
                     LYTFDRTGKLTGAVINVPCPSQNSEQEYRLSADYWHDVRSAIRARHGNIFILPQCAAA
                     GDLSPRILHYKKAQARRFRLKYGPEEKLALAERKDIAERVATSFDEVLGWAKQEIQTA
                     LPLAHRVETVQLSRRLITEEEYEYAKNGLEELKREKFRTDGTPKERLKANSILAASRN
                     RFAQVIERHETQAAEPKLPMELHVLRIGEVAFTSNRFELYMDFQHRIQARSPFEQTFI
                     VQLAGQPGAGGGSYLCTERGAWGKGYSASMFCNLVSPQGGQELVEETVRRLEALHSEP
                     ASEKA"
     gene            complement(2425..3822)
                     /locus_tag="PKN80_04770"
     CDS             complement(2425..3822)
                     /locus_tag="PKN80_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35361.1"
                     /translation="MKVINNGRFRAAIPVFLLTLTVTGGLLFKPAGLRADTAAGNEIE
                     REIQQIRERGEPVTLAELVPEEIPESENGAAVYRQAFQLFQELKIKNPERWKYIPYEG
                     GLEWPQVPAPEKAAVRNLLLAHPDFLRFYQLLEKAAGMRCQFLRRSDYRGIDTELPHL
                     ASLRSCARMLAARADLKADAGDIEGALRDCLTGFRMAGTLKEEPVIISGLVRIAIDNN
                     MLNCLEEVIQRGEATPETGRDLILKIREEREARLFYRGLLGERVLFGLPIFRRLEEKA
                     GQQAALDQLANALGATEPIKAALGADTDLAGLISENLATYLQAMNQLITFYDLPYRER
                     QAKTDALRKELLELPDRKVALVKLAMPALLGLPLNETRLDARLGNAMIALGCRRYREK
                     QGAWPESLKELSPEKAPGFLPELPLDPFTGREYVYRKRGKGFIVYSLGENLKDEGGRR
                     GEGAKRWDYDIVWQE"
     gene            4102..5388
                     /locus_tag="PKN80_04775"
     CDS             4102..5388
                     /locus_tag="PKN80_04775"
                     /inference="COORDINATES: protein motif:HMM:NF023165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HNQ35362.1"
                     /translation="MKNKSFAVRSTAWIAVILCAVFLLGAPAPARAGVENENGFSRAE
                     LAQMLAPIALYPDSLLAQVLMASTYPLEVVEAERWLRRNPGLAGDELYRALLEKDWDT
                     SVKSLCNLPEVLFVLSDDIGRTRRLGDAFLAGEKEVMDVVQELRRRAHDQGSLRTTRE
                     QQVIVEQQIIRIEPAFSHVVYVPVYDPGYVYGSWRYPACPPYAWRYPSGFSVSARYVG
                     FDPPVFISIGWFSWTSFDWPAWSIRIVFSDANRYHRYPARVDYRDDHYWRHDPRHRDG
                     VDYRDRETRDRFRPSPSPVVFRDPPPRDDRFSRGPEPTPPPKDRDNDRDIGRRERPER
                     TEVAAEPSGPSRPEAPAPVISSGRSGGDRSSDRVTDRGRGDEVRVSRGGGSEEVLEGR
                     SGRGGPSFDPGTPASSSRGRDAGPGRGKGRGGDDRR"
     gene            complement(5525..>7294)
                     /locus_tag="PKN80_04780"
     CDS             complement(5525..>7294)
                     /locus_tag="PKN80_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HNQ35363.1"
                     /translation="RPDQGRASITLNVPAERRPEETRLEIRYSPTLAAAMVDALPYLA
                     DYPYGCTEQTLNRFLPAVITQKVLIGMGLDLSEIREKRTNLNAQEIGNDRERAGQWKR
                     FDANPVFEMEELGRMVKAGVTRLTAMQNSDGGWGWFSGWGEQSWPHTTAVVVHGLQVA
                     RANDVAIVPGVLERGVEWLKKYQAEQLILIRNAEKKEKNTRWKERADDLDAFVYMVLT
                     DAGVENAAMRDFLYRDRIQLSVYAKAMFGLALHKAGDREKRDMLIRNIGQYLVQDDEN
                     QTAYLRLPEGNPWWCWYGSETEAEAYYLKLLAATDPKGQAASRLVKYLLNNRKHATYW
                     NSTRDSALVIEAFADYLKASGEDRPDLELEILVDGKRVRQVKIDRGNLFSFENKLVLL
                     GDELKTGRHTVEFRKTGTGPIYFNAYLTNFTLEDPITRAGLEIKVNRKYYRLVREDKS
                     VKAEGMHGQVLDQRVEKYRRVELADLAALKSGDLVEIELKIESKNDYEYIIFEDMKAA
                     GFEPVEVRSGYNRNALGAYMELRDERVCFFTRVLPRGTHSLSYRMRAEIPGRFSALPT
                     RASAMYAPELKANSDEIKLRVTD"
BASE COUNT         1133 a         2315 c         2381 g         1465 t
ORIGIN      
        1 gcgccaggcg gccgcgggcg cttttggtga tttcgtctat gccgagggtg agtactgcca
       61 cgatatagac gccggctgca acctgcgcca ggtggcggcc atgcgcgccg ccagcgccgc
      121 cggccaggaa tggaaacagg tgagccggaa gtaccagtca gccggcatcg agaccgggcc
      181 gatgcattac ccgacccact ccacctccgg gccggtttcg gtgatgaagg cgcacgcggt
      241 caaggtgacg gcctacggtt accggaaccg cgacgggaag ggctgcgccc aggccgacga
      301 gatcaccaac gaggtggccc tcttccggat gagcaacggc gccgcggtcc gcattgccga
      361 gctgcgcgag gcgcccggga ttctcggcca ggattccgag accttccgga tcatgggtac
      421 gcggggcagc ttctccgaga accgctggtt cgagatcgag cggccggatc cggccaggct
      481 cgacctgaat aacctgccga agccccgggt tacaaaactg acggccgccg aaatgttcga
      541 tcccctgccg ccggaggtgc aggaggcctt caagcgcgcc atgtaccgca acgagccgat
      601 tgacgagaag gatctgcaga cccgggattt cgtgccggcc ggccacggcg gctcccaccc
      661 ctacctggtg cacgagttcg tggaggcggt gaccgcgggc cggcagccgg ccatcaatat
      721 ctgggaggcg gcccgttaca tggtcatggg cgtcatggcc cacaagtcgg ccctgaagga
      781 gggcgtcacg ctcgacgtgc ccgactgggg cgacgccccg gcctgatccg gccggcggcc
      841 gccgccccct ggccgatcag gccttttcgg aagccggttc ggagtggagc gcctcgagcc
      901 gcctgaccgt ctcctcgacc agttcctgcc cgccctgcgg cgaaaccagg ttgcagaaca
      961 tgctggcgct gtaccccttg ccccaggcgc cccgttcggt gcacaggtag gagccgccgc
     1021 cggctccggg ctgcccggcc aactggacga tgaaggtctg ttcgaacggg ctgcgcgcct
     1081 ggatccggtg ctggaagtcc atgtagagtt cgaaacggtt ggaggtgaaa gcgacctcgc
     1141 cgatccgaag cacgtgcagt tccatgggca gtttcggctc ggccgcctgg gtttcatggc
     1201 gttcgatgac ctgggcgaac cggttgcggc tggcggccag gatggagttg gccttcaggc
     1261 gttccttcgg ggtgccgtcg gtccggaact tttcccgctt cagttcctcc agaccattct
     1321 tcgcgtactc gtactcctcc tccgtgatca ggcggcggct cagctgaacc gtctcgaccc
     1381 ggtgggcaag gggcagggcg gtctggatct cctgcttcgc ccagccgaga acctcgtcga
     1441 aggaggtcgc cacccgttcc gcgatgtcct tgcgctcggc gagcgccagc ttctcctccg
     1501 ggccgtactt gagccggaac cggcgcgcct gggccttctt gtaatgcagg atgcgcggtg
     1561 agagatcccc ggcggcggcg cactggggca ggatgaagat gtttccgtgc cgggcccgga
     1621 tcgcgctccg gacgtcatgc cagtaatcgg ccgaaaggcg gtattcctgt tcggagttct
     1681 gcgaggggca gggaacgttg atgaccgcgc cggtcagttt ccccgtccgg tcgaaggtgt
     1741 agagcaggtt gatgaaatgg tcggcccccg cctcgtaatg gctgaagttc gggtcgttgg
     1801 tgttgccgta catgaccgcg tgccccctgg cgccgctcct ggagttggat tccgcgccgg
     1861 gacgcttgga ggtatcgtcg aagtaaacga cccggcggct gtggccgacc accgcgtacc
     1921 cgtagccgta ggcgatgccg ccggccccgc gcttcgcgta ggcctcgcag accgcctcgg
     1981 ccgcctgtcc cgcgagaaag tgtcggtact cgtcgctcga ggcgatttcg atgccgtcgt
     2041 ggggcgtttc ggcgtacatg ttcgctaaac tattgctgga agtggaccag atggagtcgg
     2101 cgtagtggct ggcgccggtg tgcgcgtggg tggcgttcat caggatcttg gccaccggga
     2161 tttccgggtt gctctccgcg accttgtccc gtatctcgtc gagcagcccg gagcggatca
     2221 cgaccaggtc cgccgagagg aagatgacca ggtcgctccc gttgtcgatg accagcgcgc
     2281 tgacggtcag cgggtccatc accccttctg aaatccgcat gtggaactgg cccggaatgt
     2341 tgatcggctt ctccgtcgaa atgtcgcgcg ctgcccaccc gattttcagt tcgttcatca
     2401 atcgtctcct ttgaaaagtg atgtctattc ctgccagacg atatcgtaat cccatctctt
     2461 ggcgccttcg ccgcgccggc ccccctcgtc cttcagattt tcgcccaggc tgtaaacgat
     2521 aaaccccttg cctcttttcc ggtaaacata ctcccggccg gtaaagggat cgagcggcag
     2581 ttccggcagg aagcccgggg ccttctccgg ggagagttcc ttaagggatt cgggccaggc
     2641 gccctgcttc tcccggtacc ggcggcagcc gagtgcgatc atggcattgc ccagccgggc
     2701 atccaggcgg gtttcgttga gcgggaggcc caggagggcc ggcatggcca gcttgaccag
     2761 ggccaccttc cggtcgggca gttccagtaa ttccttccgg agggcgtcgg tcttcgcctg
     2821 tctttcccgg taggggagat cgtaaaaggt tattaactgg ttcatggctt gcaggtaggt
     2881 ggccaggttc tccgagatca ggccggccag gtcggtgtcg gcgcccaggg ccgccttgat
     2941 tggttcggtg gctcccaagg cgtttgccag ttgatcaaga gcggcctgct ggccggcctt
     3001 ttcctccagg cgccggaata tcgggaggcc gaacagcacc cgttcgccca gcagaccccg
     3061 gtagaaaaga cgggcctccc gttcctccct gattttcagg atcaagtccc ggccggtttc
     3121 cggcgtcgct tcgcctcgtt ggatcacctc ctccaggcag ttcagcatgt tgttgtcgat
     3181 ggcgatcctg accaggccgg agatgatgac cggttcctcc ttaagggttc cggccattcg
     3241 aaatccggtc aggcaatccc gcagggcgcc ctcgatgtcg ccggcatccg ctttcagatc
     3301 ggcccgggcc gccagcatcc gggcgcagct acgcagggag gccaggtgcg gcagctccgt
     3361 gtcaattcca cggtagtcgc ttctccgcag gaactggcag cgcatcccgg cggccttttc
     3421 cagtaattga taaaagcgga gaaagtccgg gtgggccagc aggaggtttc taacggcggc
     3481 cttttcgggt gccggcactt gcggccattc gagtccgccc tcgtacggaa tatacttcca
     3541 tctctccggg ttcttgatct tcagttcctg gaagagttga aaggcctgcc ggtagaccgc
     3601 cgcgccattt tcgctctccg ggatctcttc cggtaccagt tcggccaggg tgaccggttc
     3661 accccgttcc cttatttgct gaatctcccg ctcgatttcg ttcccggcgg cggtgtcggc
     3721 ccgcagaccg gccggtttga aaagcaggcc gccggtgacg gtaagtgtca gcaggaaaac
     3781 gggaatggcg gctcgaaaac ggccattatt tataactttc atcgtgacct cccggtcggc
     3841 gtcaatgggt tgggcctcat tttttatgct gatgatcggg ccggtgttta ttcaatatca
     3901 ggacttgcct tgataggtta tatcggcctc ctttaattgt caaggtgccc ggttattaaa
     3961 cgggcgggcg tccggcggtc tctaaaagaa taaaaggcaa gggtgaaaag ggtcgcgttc
     4021 ttctggccga aagtttttta tcgggatgtc aactctgtca tcgggggaag tcagcgcagt
     4081 caaaaaagga ggtggcgcgc catgaaaaac aagagttttg ccgtgcggag cacggcctgg
     4141 atcgcggtta ttctttgcgc ggtcttcctg ctgggagctc cggctccggc ccgggccggg
     4201 gtcgagaacg agaatggctt cagccgggcg gaacttgccc agatgctcgc cccgatcgcc
     4261 ctctatccgg actccctgct ggcccaggtg ctcatggcct caacctaccc gctcgaggtg
     4321 gtggaggccg agcgctggtt gaggcgcaac ccggggctgg ccggggacga gctctaccgg
     4381 gccctgctgg agaaggattg ggacaccagc gtcaagtccc tctgcaactt gcccgaggtc
     4441 ctctttgtct tgagcgatga catcggccgg acccggcgtt tgggcgacgc cttcctggcc
     4501 ggggaaaaag aagtgatgga cgtggtccag gaactgcgcc gccgggcgca tgaccagggc
     4561 agcctcagga cgacccggga acagcaggtc atcgttgaac aacagattat caggatcgag
     4621 ccggcctttt cgcatgttgt ctacgttccc gtctatgatc ccggttatgt ttatggttcc
     4681 tggcggtacc cggcctgtcc accttatgcc tggcgttatc cctccggatt ttcggtcagc
     4741 gcccggtatg tcggtttcga cccgccggtc ttcataagta tcggctggtt ttcgtggacc
     4801 tccttcgact ggccggcctg gagcatccgc atcgtcttca gtgacgccaa ccgttatcac
     4861 cgttacccgg cccgggttga ttaccgcgac gaccattact ggcggcacga cccgcgccac
     4921 cgggatgggg tggattaccg ggaccgggag acccgggacc gtttccggcc gtctccttcc
     4981 ccggtcgtct tcagggatcc gccgccccgg gacgaccggt tctccagggg gccggaaccg
     5041 acgccgcctc cgaaggatcg ggataatgac cgggatatcg ggcggcggga acggccggaa
     5101 cggacggaag tggctgccga accttccggg ccgagtcggc cggaagcgcc cgccccggtt
     5161 atttccagcg gccgttcggg cggcgaccgt tcctcggacc gcgtgacgga tcgtgggcgc
     5221 ggcgacgagg tcagggtttc gcgcggcggc gggtcggaag aggtcctgga aggccgctcc
     5281 ggccgtggcg ggccttcttt cgatccggga acgcctgcct cctcgtcaag aggacgggac
     5341 gctgggcccg gccggggcaa gggccgcggc ggagatgatc gcaggtagat ttggtcaggc
     5401 tgaatggaaa ggcccccggt ccgtttgggc cgggggcctt tttaatgggc cattaaagcc
     5461 gggcttcact caatcctctt cttgattctt ctccgcggcg ccaggcggct cgagccggca
     5521 cccgtcagtc cgttacccgg agcttgatct cgtccgagtt ggccttcagt tccggcgcgt
     5581 acatcgcgct ggcccgggtc ggcagggcgc tgaagcggcc ggggatctcg gcgcgcattc
     5641 ggtaactgag gctgtgcgta ccgcgcggca ggacccgggt gaagaagcag acccgctcgt
     5701 cccggagttc catgtaggcg cccagggcgt tccggttgta gccgctccgc acctcgaccg
     5761 gctcgaagcc ggccgccttc atgtcctcga agatgatgta ctcgtagtcg ttcttgctct
     5821 cgatcttcag ctcgatctca accaggtcgc cgctcttgag cgcggccagg tcggccagtt
     5881 ccacccgccg gtacttctcc acccgctggt cgaggacctg cccgtgcatt ccctcggcct
     5941 tgacgctctt atcctcccgc accagccggt agtatttccg gttgactttg atctccaggc
     6001 cggcccgggt gatggggtcc tccagggtga agttggtcag gtaggcgttg aagtagatcg
     6061 gcccggtccc ggtcttgcgg aattccaccg tgtggcggcc ggtcttcagc tcgtcgccga
     6121 gcaggaccag cttgttctcg aaactgaaga ggttgccccg gtcgatcttg acctggcgga
     6181 cccgcttgcc gtccacgaga atctccagct ccaggtccgg tcggtcctcg ccgctggcct
     6241 tcaggtagtc ggcgaaggcc tcgatgacca gcgcgctgtc ccgggtgctg ttccagtagg
     6301 tggcgtgctt gcggttattc agcaggtact tgaccagccg ggaggcggcc tgaccctttg
     6361 ggtcggtcgc cgccagcagt ttcaggtagt aggcttccgc ctcggtttcg ctgccgtacc
     6421 agcaccacca ggggttgcct tccggcagcc gcaggtaggc ggtctggttc tcgtcgtcct
     6481 gtaccaggta ctggccgata ttgcggatga gcatgtcccg cttctcacgg tcgccggcct
     6541 tgtgcagggc caggccgaac atcgccttgg catagaccga gagctggatg cggtcccggt
     6601 agaggaagtc gcgcattgcg gcattttcga ccccggcgtc cgtcagcacc atgtagacga
     6661 aggcgtccag gtcgtcggcc cgctccttcc agcgggtgtt cttctccttc ttctcggcgt
     6721 tccggatcag gatcagctgc tcggcctggt acttcttgag ccactcgacg ccgcgttcca
     6781 gcacgccggg aacgatcgcc acgtcattgg cgcgggccac ctgcaggccg tggacgacca
     6841 ccgcggtcgt gtgcggccag gactgctccc cccagccgct gaaccagccc cagccgccgt
     6901 cggagttctg catggcggtc agccgggtga ccccggcctt caccatccgg ccgagctcct
     6961 ccatctcgaa gaccgggttg gcgtcgaagc gcttccactg cccggcccgt tcccggtcgt
     7021 tgccgatctc ctgggcgttg aggttggtcc gcttctcccg gatttccgac aggtccagtc
     7081 ccatgccgat cagcaccttc tgggtgatga ccgccggcag gaaccggttg agcgtctgct
     7141 cggtgcagcc ataggggtaa tcggccaggt agggcagcgc gtcaaccatg gcggcggcca
     7201 gggtcggcga gtagcggatt tccagccgcg tctcctcggg ccggcgctcg gccggcacgt
     7261 tcagggtgat cgaagcccgc ccctggtccg gccg
//
LOCUS       DAOQYN010000091         7284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_10627_length_7284_cov_4.107484, whole genome shotgun sequence.
ACCESSION   DAOQYN010000091 DAOQYN010000000
VERSION     DAOQYN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10627_length_7284_cov_4.107484"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PKN80_04785"
     CDS             <1..816
                     /locus_tag="PKN80_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ35364.1"
                     /translation="VFDTPLSENGITGVAVGSALAGRRPVLVHQRVDFMLLTMDQLVN
                     HAAAWRRMFGGRLTVPLTIRAIIGRGWGSAAQHSQNLQALFSHVPGLKVVMPATAYDA
                     KGLLLAAIADPDPVVFLEHRWLYDTVDEVPAGAYLVPLGKAVVRRPGRSVTVAAISHM
                     AGEAEKAARLLAERGIEVEIIDLRTLRPLDLETVLASVEKTGRLVVADTGWRTGGVAA
                     EVVAAVTERGFGLLRRAPVRVACPDLPTPAGPELEKAYYPGAAEILRAVEGLR"
     gene            813..1586
                     /locus_tag="PKN80_04790"
     CDS             813..1586
                     /locus_tag="PKN80_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ35365.1"
                     /translation="MNPEISVVMPALNEEANIRAAAAAVLAAFDRFGAAGELVVVDDG
                     SRDRTGELVRELQTFDPRVNLMRHPEPLGIGASFWDGVSVARGELVVMMPGDGENEPA
                     EMLRYLALGREVDLVVPFIYNRQVRSTARNRISSLYRWIINVSFRVNFRYTNGTVLYR
                     RSVLRDLELRSRGFFYQTELLVKLVRRGYLYAEVPCRLGARSTGRSKALTLASLRKVA
                     AGYLALMREVYFRGAGPAPEPVPDSATGRRAREAALRKE"
     gene            1583..2698
                     /locus_tag="PKN80_04795"
     CDS             1583..2698
                     /locus_tag="PKN80_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014666607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ35366.1"
                     /translation="MKKTPPELILDGQKLNWHLERVQAWLRGEPVAPVTIDCALTRRC
                     NYRCVYCYGQLQANDEKRMTRDVIFRFLDDATEIGVRAISFVSDGESTCSPHLYEAIR
                     RGRANGLDLALGTNGYRLKAGELPEMLAALTYLRFNISAADPDRYAQIHGCAAGCLDR
                     VLENIRESVRIKRSAGLPVTIGLQMVLLPEYADQILPLARLGRELGVDYFVIKHCSDD
                     ESGRLGVDYRRYFDLVETLKAAEALSGPGYLVRAKWSKILSGGTRCYRQCYGPPFILQ
                     LSGSGLVAPCGMLFARKYRKYHIGNIAEKPFKEIWRSDRYREVMALLASEAFDARTMC
                     GCLCLQHKVNEFLWEIKRSGRLPSPPAGPPPEHINFI"
     gene            2740..3240
                     /locus_tag="PKN80_04800"
     CDS             2740..3240
                     /locus_tag="PKN80_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35367.1"
                     /translation="MKVLGYGLGLFLAALLAHILIWRLRRPARRIRVLCLLFPVFLAA
                     GLAWLPGRFGLPGLAPSDYLEVILLDLSLAAAYLVTYPALEVESPSMLLVLMVGRAGR
                     AGLDPADPERDLDDRRLVEPRLKDLERSRLVVCRDGCYRLTPAGRRFVGIFVGYRNLF
                     RPEGGG"
     gene            3251..3787
                     /locus_tag="PKN80_04805"
     CDS             3251..3787
                     /locus_tag="PKN80_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35368.1"
                     /translation="MLKLCAPIAGLVLNALTGIWLARRRNGLLRSLVAGFLAGLAVAL
                     FLLLAGDGSASAGSRAAAGLTYLALGYVYFHFVNMGETARRIRLLRELLAAPPGGFTG
                     AELKARYPAREIVRRRLQRLLNNGQVILADGRYRLGPRPVMYWITLVIMAWKRMLFGS
                     GWNGDGTFERLDGEAGNE"
     gene            3780..5747
                     /locus_tag="PKN80_04810"
     CDS             3780..5747
                     /locus_tag="PKN80_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ35369.1"
                     /translation="MNNRGARRGYLAAFLGLWFLCYLVYPFWLLPAPVPFRAVFFCFL
                     LAFFGLGAWGLNRLLAGRELEPPGRAMRILAGPFGHYWRENRFLLVCLLIFILLHLFP
                     NAVYPIRTCGDESTHASAGIQVMTLLSPSLESRLGLSLSWLVRAATVLAGLLYLGRRR
                     FSFGRFLVSARPARLAWLAAAVLAGLAVYFLFLCRLPFRPYLYKPTPLSRFLSLWIVS
                     VFGAREFWVRLPALLFTGLAALVLQELVALYRPRDFAGRCAAVLLFLPNVFHYAANAE
                     LAAGKLVLGLLFLFFLARHLRDDRFEDLLLAGFWAGTAFLWKSSMAVLQPVLWAALVW
                     DGLERKRPGPVWRGRLAACLLSVLPVLLSLGLEMGLVHYEMFTIVSGRHVLHLADFMT
                     YFTRLPEQVTWFGFILFCGGLASVSGRPRDWLLRLLSIYLLTWYLALTVSLAGLGMVH
                     NRWQLPAYPAVAFFIAACLEGFRRRAAATVLLLVFLASCATWLPFPGVVPEYLTYRPG
                     YLAQLAGPDAVLTERPGLLPYPEALRYIRYRLPAGRRILAVDFPDPREFYAYRYGVRV
                     DWFLLDYRLEETWRPRSGQTPAALGEFCRDNRIEYLLLPDGRWLPYLDRGLTDRLLMD
                     FTDNFELVRRFQLYGNEVYLWKTKKTAGRPG"
     gene            5711..6202
                     /gene="nusB"
                     /locus_tag="PKN80_04815"
     CDS             5711..6202
                     /gene="nusB"
                     /locus_tag="PKN80_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNQ35370.1"
                     /translation="MEDEKDGRPAGLSASKRRGGRRLARQMALSVLYQLDFRPELTPA
                     AALDTAAAEAGGDPASKEFALQLVKGVREQVGYFDELIGRYAQNWSLERVATLDRNIL
                     RLTLFEMVVGREVPPRVCIDEAVELAKRFGTEDSYRFVNGILNRIKEDLESGRLTSPR
                     KDP"
     gene            complement(6150..>7284)
                     /locus_tag="PKN80_04820"
     CDS             complement(6150..>7284)
                     /locus_tag="PKN80_04820"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNQ35371.1"
                     /translation="PGSGKKQRMRRGQRMRTAASDALGEIIAELADRAAISAHHVYEV
                     VVAGNSAMEHLFLGLPAGSLATSPYIGVCRGPVTLSARRAGLRVNEDGFVRTAPLISG
                     FVGGDTVAGILAVNLHREERLRLLVDIGTNGEIVAGNREGLFCASAAAGPAFEGANIH
                     NGMRAEPGAIDKVRITDRVEYSTIAEAPVRGICGTGLLETVSELLRVGLLGPEGRFRN
                     DSELAGLELPAAVKERLVDTPGGRAFRLDPETGVAVYQKDVRQLQLAKAAIAAGIEIL
                     LEKMSLETSDLEEVLLAGAFGSYIDPAAALRIGLLPPVRPETIRFVGNTSLAGARLML
                     LSTELKDEAECIPSLCGYVELFHEKDFMDLFVEKLAFPTPGLP"
BASE COUNT          984 a         2490 c         2425 g         1385 t
ORIGIN      
        1 gtcttcgata ccccgctctc cgagaacggg ataaccgggg tggcggtcgg ctcggccctg
       61 gccggccggc ggccggtgct ggtccaccag cgggttgatt tcatgctcct gaccatggac
      121 cagctggtca accacgcggc cgcctggcgc cggatgttcg gcggccgcct gacggtgccc
      181 ctgaccatcc gggcgattat cgggcgcggc tggggatcgg ccgcccagca ttcccagaat
      241 ctgcaggccc tcttcagcca cgtgccgggc ctgaaggtgg tgatgccggc cacggcttac
      301 gatgccaaag gattgctgct ggcggccatc gccgatcccg acccggtcgt ttttctcgaa
      361 caccgctggc tttatgatac ggtggacgaa gtgcccgccg gggcctacct ggttccgctg
      421 ggtaaggcgg tcgtccgccg gccgggccga agcgtcaccg tggcggccat ctcgcacatg
      481 gccggcgagg cggagaaggc ggcccggctc ctggctgaac ggggcatcga ggtcgagatc
      541 atcgatctgc gcacgctgcg gccgctggac ctggagacgg tgctggcctc ggttgaaaaa
      601 accggccgcc tggtggtggc cgataccggc tggcggaccg gcggcgtggc ggccgaggtg
      661 gtcgcggcgg tcaccgagcg cggtttcggc ctgttgcgcc gggcgccggt ccgggtggcc
      721 tgcccggatc tgccgacccc ggccggaccc gaactggaaa aggcctatta tcccggtgcc
      781 gcggagattc tccgggccgt ggagggactc cggtgaatcc cgagatcagc gttgtcatgc
      841 cggccctcaa tgaagaggcc aacatccggg ccgccgccgc ggcggtcctg gcggccttcg
      901 accggttcgg cgcggccggc gaactggtgg tggtcgatga cggcagccgc gaccggaccg
      961 gggaactggt ccgcgaactt cagaccttcg atccgcgggt caacctgatg cgccacccgg
     1021 aaccgctcgg catcggcgcc tccttctggg acggcgtttc ggtggcccgg ggcgagctgg
     1081 tggttatgat gccgggcgac ggggagaatg aaccggccga gatgctgcgt tacctggcgc
     1141 tcggccgcga agtggacctg gtggttccct tcatatacaa ccgccaggtg cggtcgactg
     1201 cgcggaaccg gatctcctcg ctctaccgct ggatcatcaa cgtttcgttc cgggtcaact
     1261 tcaggtacac caacggtacc gtgctttacc gccggtccgt gctccgcgat ctggaactgc
     1321 gcagccgggg tttcttctac cagaccgaac tgctggtcaa actggtccgg cgcggctacc
     1381 tttacgccga ggttccctgc cgtctcggcg cccgttcgac cgggcgctcc aaggcgctta
     1441 ccctggcctc gctccgcaag gtggccgccg gctatctcgc cctgatgcgc gaagtttatt
     1501 tccgcggcgc cggtccggcg ccggaaccgg tccccgattc ggcgaccggc cgccgggcca
     1561 gggaagccgc gctccggaaa gaatgaaaaa gacgccaccg gaactgatcc tggacggcca
     1621 gaagctcaac tggcacctcg aacgggtcca ggcctggctg cgcggcgaac cggtcgcgcc
     1681 cgtcaccatc gactgcgccc tgacccggcg ctgcaactac cgttgcgttt actgctacgg
     1741 acagcttcag gccaacgacg aaaagcggat gacccgcgac gtgatcttcc gcttcctgga
     1801 cgacgcgacc gagatcggag tcagggcgat aagttttgtc agcgacggcg agtcgacctg
     1861 ttcaccgcac ctttacgagg cgatccggcg cggtcgggcc aacggcctgg acctggctct
     1921 cggcaccaac ggctaccggc tcaaggccgg cgaactcccg gagatgctgg ccgcccttac
     1981 ctacctgcgc ttcaacatct cggcggccga tccggaccgc tacgcccaga ttcacggctg
     2041 cgcggccgga tgcctggacc gggtcctgga gaacatccgg gagagcgtcc ggatcaagcg
     2101 gagcgccggc ctgccggtga ccatcgggct ccagatggtg ctgctgcccg agtatgccga
     2161 ccagatcctg ccgctggccc gcctgggccg ggagctgggg gtggactact tcgtgatcaa
     2221 gcactgcagc gacgacgaga gcggccggtt gggcgttgat taccggcgct acttcgacct
     2281 ggtcgaaacc ctcaaggcgg ccgaggcgct ttccggcccc ggctacctgg tccgggccaa
     2341 gtggtcgaag atactcagcg gcggcacgcg ctgttaccgg cagtgctacg ggccgccctt
     2401 cattctgcag ctttccggtt cggggctggt ggcgccctgc ggcatgctct tcgcccgcaa
     2461 gtaccgcaag taccacatcg ggaacatcgc cgaaaagccg ttcaaggaga tctggcggag
     2521 cgaccgctac cgggaagtga tggccctgct ggcctccgag gccttcgacg cccgcaccat
     2581 gtgcggctgt ctctgcctgc agcacaaggt gaacgagttt ctctgggaga tcaagcgctc
     2641 cggccgcctg ccttcgccgc cggccggccc gcccccggaa cacatcaatt tcatctgacg
     2701 gttgaagagg cgcgccgttg gaagcgggga gggcgtgaga tgaaagtgct gggttacgga
     2761 ctgggccttt tcctggcggc cctgctggcg cacatcctga tctggcggct gcgccggccg
     2821 gcccggcgga tccgtgtgct ttgcctgctc tttcccgttt ttctggcggc cggcctggcc
     2881 tggctgcccg gtcggttcgg cctgccgggg ctggcgcctt ccgattacct ggaagttatc
     2941 ctgcttgatc tctccctggc cgcggcttac ctggttacct acccggctct cgaggtggag
     3001 agtccctcga tgctcctggt cctgatggtc ggccgggccg gccgggccgg tctggatccg
     3061 gccgatccgg agcgggacct ggacgaccgg cgcctggtgg agccgcggct gaaggacctg
     3121 gaacggagcc gcctggtggt ctgccgggat ggctgttacc gcctcactcc ggccgggaga
     3181 cgcttcgtcg ggatttttgt cgggtaccgc aatctttttc gtcccgaggg cggcggctga
     3241 gccgcggtac atgttgaaac tctgcgcgcc gatcgccggc ctggttctta acgcgctcac
     3301 cggcatctgg ctggcccgcc gccggaacgg cctgctgcgt tcgctggtgg ccggcttcct
     3361 ggccgggttg gccgttgcgc tgtttctgtt attggccggc gacgggagtg cctcggccgg
     3421 gtcacgggcg gcggccgggc ttacctacct ggccctcggt tatgtttact ttcactttgt
     3481 caacatgggt gaaacggccc ggcggatacg cctgctccgg gaactcctgg ccgcccctcc
     3541 cggtgggttt accggggccg aactcaaggc ccgctacccg gcccgggaga tcgtccggag
     3601 gcgcctgcag cgcctgttaa acaacggcca ggtgatcctg gccgacggcc gctatcggct
     3661 ggggccgcgg ccggtcatgt actggataac gctggtgatc atggcctgga aacgaatgct
     3721 gttcggaagc ggctggaacg gggacgggac ttttgagcgc ctggacgggg aggccggcaa
     3781 tgaataaccg gggcgcccgg cgcggctacc tggccgcctt cctgggactc tggttcctct
     3841 gttacctggt ctaccccttc tggctcctgc cggcgccggt cccgttccgg gcggtcttct
     3901 tctgtttcct gctggccttc ttcggactcg gcgcctgggg attgaaccgg ctgctggccg
     3961 gccgcgaact ggagccgccg ggccgggcca tgcggattct tgccggtccc ttcggacact
     4021 actggcgcga aaaccggttc ctcctggtct gcctgctgat tttcatcctg ctccatcttt
     4081 tcccgaacgc cgtctacccg atccggacct gcggagacga atcgacccat gcctcggccg
     4141 gcatccaggt gatgaccctg ttatccccgt ctctggagtc gcgcctgggc ctgtccctct
     4201 cctggctggt ccgggccgcc accgttctgg ccggcctgct ttacctgggg cgccggcgtt
     4261 tctccttcgg ccgattcctg gtttcggccc ggccggcccg gctggcctgg ctggccgcgg
     4321 ccgtactggc cgggctggcc gtctacttcc tgttcctctg ccggctgccc ttccgtccct
     4381 acctttacaa gccgaccccg ctgagccgtt ttctttccct ctggatcgtc tcggtcttcg
     4441 gggcccggga attctgggtc cgcctgccgg cccttctctt caccggcctg gccgccctgg
     4501 tcctgcagga gctggtcgcc ctctaccgtc cgcgcgattt cgccggccgc tgcgcggccg
     4561 tcctcctgtt ccttcccaac gtttttcact atgccgccaa cgccgagctg gccgccggca
     4621 agctcgtcct gggcctcctc ttcctattct tcctggcccg ccacctgcgg gatgaccgtt
     4681 ttgaagacct cctgctggcc ggtttctggg ccgggaccgc ctttctctgg aagagctcca
     4741 tggcggtgct ccagccggtg ctctgggcgg cgctcgtctg ggacggcctg gagcggaagc
     4801 ggcccggccc ggtctggcgc ggccgtctgg ccgcctgcct gctgtccgtc cttccggtcc
     4861 tgctttcgct gggcctggaa atgggcctgg tccactacga gatgttcacc atcgtcagcg
     4921 gtcggcacgt cctgcacctg gccgatttca tgacctactt cacccgcctt ccggaacagg
     4981 ttacctggtt cggcttcatt ctcttctgcg gcggcctggc ctcggtctcc ggccggccgc
     5041 gggattggct gctgcgcctg ctgtccattt acctgctgac ctggtacctg gccctgaccg
     5101 tttccctggc cggcctgggt atggttcaca accgctggca gctgccggcc tacccggcgg
     5161 tggccttctt catcgccgcc tgcctggagg gcttccggcg ccgcgcggcc gcgaccgtac
     5221 tgctgctggt cttcctggcc tcctgcgcca cctggcttcc cttccccggg gtggtgcccg
     5281 agtacctgac ctaccgtccg ggttacctgg cccagctggc cggtcccgac gcggtcctta
     5341 ccgagcgtcc cgggttgctg ccctacccgg aggcgctccg ttatatccgt taccggctgc
     5401 cggccggccg ccggatactg gcggtggatt ttccggatcc gcgcgagttt tatgcttacc
     5461 gttacggggt ccgggtggat tggttcctgc tcgattaccg actggaggag acctggcggc
     5521 cgcgctccgg gcagacgccg gccgcactcg gcgagttctg ccgggacaac cggatcgagt
     5581 acctgctgct gccggacggc cgctggctgc cctacctgga ccggggactg accgaccggc
     5641 tcctgatgga ttttacggac aacttcgagc tggttcggag gtttcaactc tacggcaacg
     5701 aggtttatct atggaagacg aaaaagacgg ccggccggcc gggctgagcg cttcgaaacg
     5761 gcggggcggc cggcggctgg cgaggcagat ggccctgagc gtactttacc agctggactt
     5821 ccgtcccgag ctgacaccgg cggcggccct ggacacggcg gcggccgagg ccggcggcga
     5881 cccggcgtca aaggagttcg ccctgcagct ggtgaaggga gtccgcgaac aggtcggtta
     5941 ttttgacgag ttgatcggcc ggtacgccca gaactggtcg ctggaacggg tggcgacgct
     6001 ggaccgcaac atcctgcgcc tgacgctttt tgaaatggtg gtgggcaggg aggtgccgcc
     6061 ccgggtttgc atcgacgagg cggtggagct ggccaagcgg ttcggcaccg aggattccta
     6121 ccgcttcgtg aacggaatcc tgaaccgcat caaggaagac ctggagtcgg gaaggctaac
     6181 ttctccacga aaagatccat gaaatccttt tcgtggaaca gctccacgta gccgcagagg
     6241 cttggtatgc actcggcctc gtccttcagc tcggtggaaa gcagcatcag gcgcgccccg
     6301 gccagcgagg tgttgccgac gaaccggatg gtttcgggcc ggaccggcgg cagcagcccg
     6361 atgcgcaggg ccgccgccgg atcgatgtag gagccgaagg cgcccgccag caggacctcc
     6421 tcgaggtcgg aggtttccag cgacatcttc tccagcagta tctcgattcc ggccgcgatg
     6481 gccgccttgg ccagctgcag ctggcggacg tctttctggt agaccgccac cccggtctcc
     6541 gggtcgagtc ggaaggcccg gcccccggga gtgtcgacca ggcgctcctt cacggccgcc
     6601 ggcagttcca gcccggccaa ttccgaatcg ttccggaagc gtccttccgg tcccagcagg
     6661 cccaccctca acagctcgct gaccgtttcc agcaggccgg ttccgcagat gccccggacc
     6721 ggcgcctcgg cgatggtgct gtactcgacc cggtcggtga tccgaacctt gtcgatggcg
     6781 cccggttccg ccctcatgcc gttgtgtatg ttggccccct cgaaggccgg cccggccgcc
     6841 gccgaggcgc agaagaggcc ctcccggttg ccggccacga tttccccgtt ggtgccgatg
     6901 tcaaccagca gccgcagccg ttcctcccgg tggagattga ccgccaggat gccggcgacc
     6961 gtgtcgccgc ccacgaagcc gctgatcagg ggcgcggtgc gcacgaagcc gtcttcgttg
     7021 acgcgcaggc cggcccggcg ggccgagagg gtgaccggcc cccggcagac cccgatgtag
     7081 gggctggttg ccaggctgcc ggccggaagg cccaggaaga gatgttccat ggcgctgttg
     7141 ccggccacca ccacctcgta aacatgatgt gcgctgatgg cggcccggtc ggccagttcg
     7201 gcaatgattt cgcccagcgc gtccgatgcg gccgtccgca tccgctggcc gcgccgcatc
     7261 ctctgcttct tccccgaacc cggc
//
LOCUS       DAOQYN010000092         7255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_10674_length_7255_cov_4.949861, whole genome shotgun sequence.
ACCESSION   DAOQYN010000092 DAOQYN010000000
VERSION     DAOQYN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10674_length_7255_cov_4.949861"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="yidC"
                     /locus_tag="PKN80_04825"
     CDS             complement(<1..1028)
                     /gene="yidC"
                     /locus_tag="PKN80_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNQ35372.1"
                     /translation="MDKRVLLASILSLLVLVTFQYWQTKTAPRPTAPAVMGAAPDQGP
                     ADTAPADAPAVAAAPAVQPLPAAAAAPIGREVIVETDLYRAVFTTRGGKLVSLGLKHY
                     LRRKKPPFQAENETRFETLRPDGQPAADLTVLPFELVGSDTMLYRPSTESLLLSKSNP
                     KGELVLQAESGGQPITKRFRFDNDRYGLELEMSGAAAPGLDLGAFSLAPPTSGQKQPE
                     ELIFAADGKISRLRAGGRNKNLPLEQKSVLYLARQGQYLLTILKPIETLPARIEADAY
                     GNLGARFRAAGGPGRGNRFYFYSGPSDYQILKREGVGIPEIMPSGPMVALSRFIMSIL
                     TFFHRIIP"
     gene            complement(1058..1267)
                     /gene="yidD"
                     /locus_tag="PKN80_04830"
     CDS             complement(1058..1267)
                     /gene="yidD"
                     /locus_tag="PKN80_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNQ35373.1"
                     /translation="MKKVLIRLVELYRLAVSPFLPRSCRYYPSCSEYARAALEKHGAL
                     RGGRLAAGRLLRCHPFHPGGYDPVP"
     gene            complement(1267..1401)
                     /gene="rpmH"
                     /locus_tag="PKN80_04835"
     CDS             complement(1267..1401)
                     /gene="rpmH"
                     /locus_tag="PKN80_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNQ35374.1"
                     /translation="MSITFNPHNRKRKRTHGFLKKMRTPGGRNVLNRRRAKGRKRLAV
                     "
     gene            complement(1495..2178)
                     /locus_tag="PKN80_04840"
     CDS             complement(1495..2178)
                     /locus_tag="PKN80_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNQ35375.1"
                     /translation="MESPSLWLALAAGIGYFFSPCTLPLLPSYLVYLSGVSLPEIGGR
                     KRFAVAAHAALFLAGFLTLFVLLGASATLLGSLLGRQARLIQSLGGVVVVLLGLALVF
                     KPGLLYRLPSGGGRNSAARPAGYFGSFLVGLSFSAAWTGCSSPILGAILVTAATADTV
                     RQGIRLLVAFSLGLALPFLVSALLIDVLLAPLRRFQRHLRKVEVALGILFIAFGLYLA
                     LRSFPFVWG"
     gene            complement(2200..2595)
                     /locus_tag="PKN80_04845"
     CDS             complement(2200..2595)
                     /locus_tag="PKN80_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007466243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35376.1"
                     /translation="MFIALLIVNFLVALLVCTVIVRVFGDSIDRILRRLVQEDIYNAW
                     VRYITFTIYVVGVSGGVRIWMLEKYISPQAAGGTMLELNWDRWTLELYRTVIDTMQSV
                     AWMLLLFFLFALIAYVIVKGLEGRRAAKP"
     gene            complement(2638..3429)
                     /locus_tag="PKN80_04850"
     CDS             complement(2638..3429)
                     /locus_tag="PKN80_04850"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HNQ35377.1"
                     /translation="MKMDKRFLVEVGMKDLPFPMRVQSKADPEGQATVASIDIRARIM
                     REFEARWIDTFIQVLHQHRERIGTRTLRTNILDYLKKLKADMVRVDFRYPFFAAKETP
                     VSREKCLVCYRCTYSVKVAAADAHPKTFFAIEIPVITTYPGSSDRVPGGLFGQLSLVE
                     IEVESEKEVYPEDLVEPTDRQALAPVYSYLTPEDQLAIITRVHSEAKTSVAMADGIRA
                     ELARRKSIHWYSVRVANFGMLHSYSTVIQTEKSMWVPFSGGDEEP"
     gene            3677..5062
                     /locus_tag="PKN80_04855"
     CDS             3677..5062
                     /locus_tag="PKN80_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35378.1"
                     /translation="MKTGRGAHLAAGAAKVDISPELGIQIAGDIGRRRPCTALKEPIY
                     ARALAFESGQERFCVLTLDVLAIDNPWSDEIRRRVQASFGLPAGSLLIHALQNHAAPS
                     IGDHFCREECRLIPDSERWLRGGDQAYNEPAMAGAVTAVGQALANLVPVTLSFGRATD
                     GRVSFNRRFVMRDGRVVCHPPDCSPEILHAEGLTDPEVSLAVFTDEAGQIVSALMHHT
                     CHPCHGFDDNELLAGWPGAWCREMEAYFGPSCIPLVLNGCCGNIHHHNHLAPDWKDDH
                     LWMGRRLAESSFRALARPISLDPFPFAWKRRILPVPHRRIPARLLAEARKLLRKHPEP
                     LWKDAKHIRVEWDWVYAVGIMDIADERRSSPDYPCEIQALRLGNLALLALMGEPFVEA
                     QLRIKRESPFPFTQVAHMCNGDLGYVPTRRAFAGGGYETRIGRVSRLEPRALEMVEAA
                     ALDLLRELRSA"
     gene            5133..6059
                     /locus_tag="PKN80_04860"
     CDS             5133..6059
                     /locus_tag="PKN80_04860"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNQ35379.1"
                     /translation="MTLLNRFRGCLLGLAVGDAVGTALEFKPRGTFQPIAGLAGGGPF
                     NLRPGEWTDDTSMALCLAASLVEKGEFDPRDQMERYCRWEEEGYLSSNGRCFDIGAAT
                     AAALNRYRATGEAFAGSADPNSAGNGSIMRLAPVPLFFFPDPAAIEKYAVASSRTTHG
                     AAECLDACRFLASVISRLLQGKGREEALFGEAPAFRGAPKIEVVARGDYRDKEETAIR
                     GSGYVVESLEAALWCFLRADSFEGAVLRAANLGDDADTTAAVCGQVAGAFYGEARIPA
                     GWLAKLARREEIRGLADRLYEGNPNLTRPAGP"
     gene            complement(6066..7226)
                     /locus_tag="PKN80_04865"
     CDS             complement(6066..7226)
                     /locus_tag="PKN80_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35380.1"
                     /translation="MKTVKKGRGGVGEQTVRIALEKEKARQLLDGAYGVLSDAYNNLG
                     LTHEELGQNKKALAAYKQAVCFNPEFAAAHYNQGNVYGKLKQEKQALAAYKKAIRIQP
                     DFLLAYRNLIIAYMKLGRYGEGIKVAEQAIRLNLDDADFYATLSGLHIYLRQYEKAAA
                     AGRKAVELDPDSASAWHNLGTACMELGRYREAVEAYRHLLRVKPRDAEDQASLSCALT
                     HVGQPEAAIEAAKRALEIREEYWMGYANWAFALLKQGRNDDAIPLYRKALEKKPPEFD
                     EYIICWELGWLYFLVGDYRHALKYTDRAIGLSKEPARVIFFNRGLINLAAGRDKEALK
                     TYRKAVKAARQKGDGQVIATGRQDLEDFIKARGPAAGVKGTAEEILNLMAGK"
BASE COUNT         1200 a         2324 c         2349 g         1382 t
ORIGIN      
        1 ttgggaatga tccggtggaa gaaggtgagg atcgacatga tgaagcggct cagggcgacc
       61 atcggaccgg agggcatgat ttccggtatg cccacgcctt cccgcttcag gatctggtaa
      121 tccgaggggc cggagtagaa gtaaaaacgg ttgccgcggc ccggtccgcc ggcggcgcgg
      181 aagcgggcgc ccaggtttcc ataggcatcc gcttcgatcc gggccggcag ggtttctatc
      241 ggcttcagga tggtcagcag gtactgcccc tgtcgggcca ggtagaggac cgatttctgt
      301 tccagcggga ggtttttgtt gcgcccgccg gcccggagcc ggctgatctt gccatcggcg
      361 gcgaagatca gttcctcggg ctgcttctgg cccgaggtgg gcggcgccag gctgaaggcg
      421 cccaggtcaa ggccgggcgc ggccgcgccc gacatttcca gttccaggcc gtagcggtcg
      481 ttgtcaaagc ggaagcgctt ggtgatgggc tggccgccgg attcggcctg gagaaccagc
      541 tcgcccttcg ggttggattt ggagaggagc agggactcgg tgcttggccg gtagagcatg
      601 gtgtcggaac cgaccagctc gaaggggagc acggtcaggt cggccgccgg ctggccgtcc
      661 ggccgcaggg tctcgaagcg ggtctcattc tccgcctgga agggcggttt cttccggcgc
      721 aggtaatgtt tcagcccgag ggagaccagc ttgccgccgc gggtggtgaa gaccgcccgg
      781 tagagatcgg tttcgacgat tacctcgcgc ccgatcgggg cggccgcggc cgccggcagc
      841 ggctggaccg ccggtgccgc cgccactgcc ggcgcgtcgg ccggggcggt gtcggccggg
      901 ccttgatccg gcgccgcgcc catcacggcc ggcgccgtcg gccggggagc ggtcttcgtc
      961 tgccagtact ggaaagtaac cagcaccagc agggagagta tcgaggccag caggactctt
     1021 ttatccataa agaagcttca gggtgttcgc gtggaattta cgggaccggg tcgtagccgc
     1081 ccggatgaaa aggatggcag cgcagcagcc gcccggccgc cagccggccg cctcggagcg
     1141 cgccgtgctt ttccagggcc gcgcgggcgt attccgagca ggacgggtag tagcggcagg
     1201 aacggggcag gaacggcgaa acggccagcc ggtaaagctc gaccagccgg atcaggacct
     1261 tcttcatcag accgccagcc gtttccttcc cttggcgcgg cggcggttca gaacgttgcg
     1321 cccgccgggc gtgcgcatct tcttcaggaa accgtgcgtt ctctttcgct tccggttgtg
     1381 ggggttaaag gtaatgctca tcggacctcc ggtttttgaa attttaaata atgattatac
     1441 cataaagggc cgccggaccg gaaacccggt cgggttgacc gccggcgccc cggcctaacc
     1501 ccagacgaac gggaaactgc gcagggcgag atagagcccg aaggcgatga agagaattcc
     1561 gagcgccacc tccaccttgc gcagatggcg ctggaagcgg cgcagaggcg ccagcaggac
     1621 gtcgatcagc agcgccgaga ccaggaaggg cagggccagg cccaggctga aggcgaccag
     1681 cagccggatt ccctggcgca ccgtgtcggc cgtcgccgcg gtcaccagga tggcgcccag
     1741 gatcgggctg ctgcagccgg tccaggccgc cgagaaagat agcccgacca ggaaggagcc
     1801 gaaatagccg gccggccggg ccgcgctgtt ccggccgccg ccggagggca gccggtagag
     1861 caggccgggt ttgaagacca gggccagtcc cagcaggacg accaccacgc cgcccaggct
     1921 ctggatgagc cgggcctggc ggcccagcag gcttcccagc agggtcgccg aggcgcccag
     1981 cagcacgaag agggtcagga atccggccag gaagagggcg gcgtgggccg ccaccgcgaa
     2041 acgcttccgg ccgccgatct ccggcagcga aacgccgctc aggtagacca ggtaggaagg
     2101 gagcaggggc agcgtgcagg gtgagaagaa gtagccgatg ccggcggcca gggccagcca
     2161 gagcgagggt gattccatgt ttccggctcc tccgggtgat cagggtttgg cggcccgccg
     2221 cccctccagg cccttgacga tcacgtaggc gatcagggcg aaaaggaaga agagcagcag
     2281 catccaagcc acgctctgca tggtgtcgat taccgtccgg taaagttcaa gcgtccagcg
     2341 gtcccagttc agttccagca tcgtgccgcc ggccgcctgg ggtgagatgt acttctccag
     2401 catccagatg cgcaccccgc cggagacgcc gaccacgtag atggtgaagg tgatgtagcg
     2461 gacccaggcg ttgtagatgt cctcctggac cagccggcgc aggatccggt cgatcgagtc
     2521 gccgaagacc cggacgatga ccgtgcagac cagcagggcc accaggaagt tgactatcag
     2581 cagcgcaatg aacatctccg cctcccgttt tcgtttcctc tccggccgcc ggcgtcttta
     2641 cggctcctcg tcgccgccgc tgaaggggac ccacatactc ttctcggtct ggatgacggt
     2701 gctgtaggaa tgcagcatgc cgaagttggc cacccggacc gagtaccagt ggatcgattt
     2761 ccggcgggcc agctcggccc ggatgccgtc ggccatcgcc acgctggtct tcgcttccga
     2821 gtgcaccctg gtgatgatgg ccagctggtc ctccggggtg aggtaggagt aaaccggcgc
     2881 cagggcctgc cggtcggtcg gctccaccag gtcctcggga tagacctcct tctccgactc
     2941 cacctcgatc tccaccaggc tcaactggcc gaagaggccg ccgggcaccc ggtccgacga
     3001 gcccgggtag gtggtgatga ccgggatctc gatggcgaag aaggtcttcg ggtgggcgtc
     3061 ggccgcggcc accttgaccg aataagtgca ccggtagcag accaggcact tctcccggct
     3121 gaccggcgtc tccttggccg cgaagaacgg gtagcggaaa tcgacccgga ccatgtcggc
     3181 cttcagcttc ttcaggtaat ccaggatatt ggtccgcagc gtccgggtgc cgatgcgttc
     3241 ccggtgctgg tgcagaacct ggatgaaggt gtcgatccag cgcgcctcga actcgcgcat
     3301 gatgcgcgcc cggatgtcga tgctggctac ggtggcttgt ccctcggggt ccgccttcga
     3361 ctgaacccgc atcgggaacg gcaggtcctt catgcccacc tcgaccagga atcgcttgtc
     3421 catcttcatg ggcccctctc ccgaaccgat tttttaatat gttattgagt cgtttttccg
     3481 gttgttgccg gcctcccccc attttcatta taatgcatcc cgggattttc ctccacctta
     3541 taattattcg cttttctttt ggaaaccgac cgggttatac taccacctgg tgtcccggtt
     3601 gaatcccggc tgctggttgg tttgattgac tggaatcttt ccggtatttg ttttgaagcg
     3661 gcaataagga ggcgaagtga aaactggtcg aggtgcgcac ctggccgctg gcgcggcaaa
     3721 agttgatatc tctcccgaac tcggtattca gatcgccggt gatatcggcc gccggcgtcc
     3781 ctgcaccgct ttgaaggaac ccatctacgc ccgggctctg gctttcgagt cgggccagga
     3841 gaggttttgc gtcctcactc tcgatgtgct ggccatcgac aacccctggt ccgacgaaat
     3901 ccgccgccgg gtccaggctt cctttggcct gccggccggc tcgctgctga ttcatgcttt
     3961 gcaaaatcac gccgcgccaa gcatcggcga tcacttctgt cgggaagagt gccgactcat
     4021 tcccgattcc gagcgctggc tgcgcggcgg cgaccaggct tacaacgagc cggcaatggc
     4081 cggcgcagtg acggccgtcg gccaggccct ggcgaatctg gttccagtta cgctctcctt
     4141 tggtcgggcc accgacggcc gagtttcctt caaccgtcgc ttcgtgatgc gcgacggccg
     4201 agtcgtctgc cacccgccgg actgtagccc ggagatcctt cacgccgaag gactgactga
     4261 cccggaagtc agcctggctg tcttcaccga cgaagcagga cagatcgtct cagctctgat
     4321 gcaccatacc tgccatccct gccacggctt tgatgataac gaacttcttg ccggctggcc
     4381 cggcgcctgg tgccgagaga tggaggctta tttcggccct tcctgcattc ccctggtcct
     4441 caacggctgc tgtggcaaca tccatcatca caaccacctg gccccggatt ggaaggacga
     4501 ccatctctgg atgggccgcc ggctggccga atcatccttt cgggccctgg cccggccgat
     4561 ctcccttgat ccgtttccct tcgcctggaa gcgccgtatc ctgccggtgc cccatcgccg
     4621 cattccggcc cggctcctgg ctgaggccag gaaattgttg cggaagcacc cggaaccgct
     4681 ctggaaagat gcaaagcata tccgggtcga atgggactgg gtctatgcgg tcggcattat
     4741 ggacatcgcc gacgagcgcc gctcttctcc cgactatcct tgcgaaatcc aggccctgcg
     4801 ccttggcaac ctggccttgc ttgcgctgat gggcgaaccc tttgtcgagg cccagctgcg
     4861 catcaagcgc gaatcgccct ttccctttac ccaggtcgcc cacatgtgca acggcgacct
     4921 gggttatgtg cccactcgcc gcgccttcgc cggcggtggc tacgagaccc gcatcggccg
     4981 tgtttcgcgc ctggagccgc gggccctcga gatggtcgag gccgccgccc tcgacctgct
     5041 gcgggagctg cggtccgcct gaggcggggt tggattttgg gaccggcggg cgcggagacg
     5101 gccgggcgga tcgtaagtct tgaggattaa agatgacctt attaaaccgt tttcgcggct
     5161 gcctgctcgg gctggccgtt ggcgacgcgg tcggcaccgc cctcgagttc aagccccgcg
     5221 ggacgttcca gccgatcgcc ggcctggccg gcggcggtcc tttcaacctg cggcccggcg
     5281 agtggaccga cgacacctcg atggccctct gcctggcggc cagcctggtc gaaaaaggcg
     5341 aattcgaccc gcgcgaccag atggagcgct actgccggtg ggaagaagag ggatacctct
     5401 ccagcaacgg ccgctgtttc gatatcggcg ccgccaccgc cgcggccctg aaccgctacc
     5461 gcgcaaccgg ggaagcgttc gcggggtccg cggacccgaa ctccgccggc aacggttcga
     5521 taatgcgcct ggcgccggtc ccgctcttct tctttcccga ccccgcggcg attgagaagt
     5581 atgcggtggc gagctcccgc accacccacg gcgcggccga atgtcttgac gcctgccgct
     5641 ttcttgcctc ggtgatcagc cgccttctcc agggaaaggg gcgggaggaa gccctttttg
     5701 gagaggcgcc cgcgttccgg ggcgcgccga agatcgaggt cgtcgcccgc ggcgattacc
     5761 gcgataagga ggaaacggcc atccggggtt cgggttacgt ggtcgagtcc ctcgaggcgg
     5821 ccctctggtg ttttctccgg gccgattcct ttgaaggggc ggtgctccgg gccgccaacc
     5881 tcggcgacga cgccgacacc acggcggccg tctgcggcca ggtcgccggg gccttttacg
     5941 gcgaggcgag gatcccggcc ggctggctcg cgaaactcgc ccggcgcgaa gagatcaggg
     6001 ggctggccga ccgcttgtat gaaggcaacc cgaacttgac gcggccagcc ggaccttgaa
     6061 cggccttatt ttccggccat caggtttaaa atctcttcgg ccgttccctt tacccccgcg
     6121 gccggtcccc tggccttgat gaaatcctcg aggtcctgtc gccccgtcgc gatcacctgg
     6181 ccgtcacctt tttgacgggc cgctttgacc gctttccggt aggttttgag cgcctcttta
     6241 tcgcgccccg cggcgaggtt gatcagcccc cggttgaaga agatgacccg ggccggttcc
     6301 ttgctcaacc cgatcgcccg gtcggtatat ttcagcgcgt gcctgtaatc gccgaccagg
     6361 aaatacaacc agcccagctc ccagcagata atatattcgt cgaattcagg cggtttcttc
     6421 tccagcgcct tccggtacaa ggggatggcg tcgtcgttcc ggccctgctt gagcagggcg
     6481 aaggcccagt tggcgtatcc catccaatac tcctcgcgga tttccagcgc tcgtttggcc
     6541 gcctcgatgg ccgcctccgg ctggccgacg tgggtcaggg cgcaggagag cgacgcctgg
     6601 tcctcggcat ccctcggctt gacccggagc agatgccggt aggcctccac cgcctcccgg
     6661 tatcggccga gctccatgca ggccgtgccc aggttgtgcc aggcgctggc ggagtcgggg
     6721 tcgagctcga ccgctttccg gccggccgcc gccgcctttt cgtactggcg gagatagatg
     6781 tggagtccgc tcagggtggc gtagaaatcg gcgtcatcga gattgaggcg gatcgcctgc
     6841 tcggcgacct tgatcccttc cccgtatcgg ccgagtttca tataggcgat gatcaggttg
     6901 cggtaggcca ggaggaaatc cggttgaatc cggatcgcct ttttgtaggc cgccagggct
     6961 tgcttctcct gcttgagttt gccataaacg ttcccctggt tgtagtgggc tgcggcaaat
     7021 tcggggttga agcacaccgc ttgtttgtag gccgccaggg cttttttatt ctggcccaac
     7081 tcttcatggg tcagccccag gttgttataa gcatccgaga gcacgccgta agcgccatcc
     7141 aggagctggc gcgccttttc tttttccaag gcgatgcgga cggtctgctc gccaacccct
     7201 ccacgccctt ttttcaccgt tttcattttt cagtcccccg gtttttacgc gttcg
//
LOCUS       DAOQYN010000093         7137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_10884_length_7137_cov_4.122564, whole genome shotgun sequence.
ACCESSION   DAOQYN010000093 DAOQYN010000000
VERSION     DAOQYN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10884_length_7137_cov_4.122564"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1177
                     /locus_tag="PKN80_04870"
     CDS             146..1177
                     /locus_tag="PKN80_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNQ35381.1"
                     /translation="MEPRYTRPGPRPNRILIIISGFVALYLVNLWMSGELYTVNLALK
                     NPRLQLFGVIMTAILLEGFPFLLLGSCISGAIEVLVPREVLEKRLFSANPIKAAAVGS
                     LLGMAFPVCSCGNIPVTRRLMSKGVPVAGAVSYLLAAPIINPITLFSTIAAFPQEKTV
                     VIGRLTLAFIIANIAGLCLGRWKPGEILKAEAGHDLCPHEHHHHDHSGNRLGRILSHA
                     ESDFFLTGRYLIMGASVAALFQTFIPRTAVNALAANSLLSVFVMELMGIAFSLCSFAD
                     AFVASTFTALPAAAKTGFMVAGPMTSLALVTLYLGTFQRKFAGRLIMITAAGVLILAS
                     VWALLSIGG"
     gene            1181..1885
                     /locus_tag="PKN80_04875"
     CDS             1181..1885
                     /locus_tag="PKN80_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HNQ35382.1"
                     /translation="MNKQWWLRAGVILTWFTFIVIILAGGYLPMYIRKSYAPLAFVAL
                     VILALMAIQPETSGAGHGHQESISRSSLLIFLLPVLFVAVVRPGTLSSLAVQTRGIST
                     ALAGTDASLAESLAARVATESGYRSMNLKQLLTLVSGSPEKADALPIAVEGMVFKNSS
                     HAPGTFMLVRFLITCCAADATPLGIAVSWDAASGPPPENAWVLVRGKISFSEATPSVI
                     ADEIQVMPNPANPYLY"
     gene            2046..2909
                     /locus_tag="PKN80_04880"
     CDS             2046..2909
                     /locus_tag="PKN80_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ35383.1"
                     /translation="MTIAIASGKGGTGKTTVAVNLARIFETEVQLLDCDVEAPNAHLF
                     LKTGNRKEAAVAIRVPEVDEKICDGCGECGRFCAYNAIAVFGPRPMVFPEMCHGCGGC
                     ALVCPKKAIRETDRRIGVIETSRTGNITLIQGCLDIGVAMAPTLIRAVKARRQTDRPA
                     ILDAPPGTSCPVITTLRKADFALLVTEPTPFGLYDLTMAVEMIRELKIPFGVIVNRVG
                     VGDDRVHAFCRETGIPILLEIPDDRRIAEAYSRGEILVEARPEYRALFTGLLEKIKKA
                     GGNNRLKEGKE"
     gene            2906..3226
                     /locus_tag="PKN80_04885"
     CDS             2906..3226
                     /locus_tag="PKN80_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNQ35384.1"
                     /translation="MKLGIVITRTDPESVFNVLRLALYSLEHGDRVRIFLSGRGVEID
                     RIEDPKFDVKGLAQKVLNAGGSFHACGSCLKLRNSNGSDICPLSTLKDHYEIVRDSDR
                     LVTI"
     gene            3223..4125
                     /locus_tag="PKN80_04890"
     CDS             3223..4125
                     /locus_tag="PKN80_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ35385.1"
                     /translation="MKEGKTKTRELVVVSGKGGTGKTSLTAAFAALAEAPVVADCDVD
                     ATDLHLLLSPRILERHRFRSGHEAVIRQEDCVGCGGCLAYCRYDAVKRTIQPAGDAVF
                     SIDPVACEGCGVCVRFCPENAIDFPERECGEWMISESRCGPMVHAKLGVAAENSGKLV
                     SIVRREARRLADAENRRLIIVDGPPGIGCPVIASMTGATLVLAVTEPTLSGEHDLKRV
                     LSLAAHFRIPAAVCVNKWDLNPDLAGRIETGAARAGARIVGRVRYDRSVTLAQMKKLT
                     VVETEAPCAEDVRSVWQNLNLEKA"
     gene            4122..4982
                     /locus_tag="PKN80_04895"
     CDS             4122..4982
                     /locus_tag="PKN80_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNQ35386.1"
                     /translation="MKNNEFNDQVQLQSRLERIGHKIIILSGKGGVGKSTISVNLAVS
                     LMLEGKRVGLLDVDIHGPSIPTMLGLEKEILRADEDGLFPIELGDLKVMSIGFLLKNQ
                     DEAVIWRGPMKMGAIKQFLQDVAWGDLDYLIIDSPPGTGDEPLSVCQLIGGLDGAVVV
                     TTPQKVAAVNVRKSVNFCRQLKVPVIGIVENMSGFACPKCGQVTPILREGGGRRIAAD
                     MKTPFLGAIPIDPEIALSGDEGRPFIHHYAASPTAGIMREIIKPILALDRTGHPRPNG
                     KTTSPTEEKK"
     gene            4979..5317
                     /locus_tag="PKN80_04900"
     CDS             4979..5317
                     /locus_tag="PKN80_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNQ35387.1"
                     /translation="MKIAIPLAAGRLSMHFGHCEQFALLDVDPEKKSILNRADVTPPP
                     HQPGLLPPWLAEQGVTLVIAGGMGQRAQDLFARQGIKVVIGAPAESPEKLAADFLSGN
                     LKEGENVCDH"
     gene            5600..6433
                     /locus_tag="PKN80_04905"
     CDS             5600..6433
                     /locus_tag="PKN80_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ35388.1"
                     /translation="MAASVRITILVDNQAGEGLAAEHGFALWIETGGKNILFDTGEGP
                     ALLHNAPQLGVDLATADCLVLSHGHHDHTGGVAALLEKTGPLDVYFHPGLTDERYAIR
                     SGKAISIKMPPASAAALANLPEARKHPVPAPAEIFEGVGLTGPIPRRTAYEDTGGPFF
                     LDPAAGRADPIEDELALWIRTSGGLVVCLGCGHAGLVNTLNHVRRLNGEEKIRAAIGG
                     FHLVESEERRFDLTGAALTGLAPELVIPCHCTGDRAVEALKSRLGKRVITGRSGNSFE
                     F"
     gene            6669..>7137
                     /locus_tag="PKN80_04910"
     CDS             6669..>7137
                     /locus_tag="PKN80_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35389.1"
                     /translation="MITPRENMLKIFRHERPDWLPLTGHCDPYNQPSREGMDPALASA
                     LGEVKWGDESIVTFSRYLGLDILDWFGLPSVRSSRRRVTMEEIVEGDVKTHVWHTPRG
                     DLRETIRVLRDPSGARSSNYTEHLVKSPADLPALAEIFEDELIESDPDGFEKTR"
BASE COUNT         1383 a         2363 c         2131 g         1260 t
ORIGIN      
        1 cccgcaatgg ctggcaggac caggtggacg cggaaaccgc ccgccgcctg ggccgccgcg
       61 gctatcgggg cgcgcccaat cgtcgccgcg taccggtcta aaaacactcc cgcccaaaaa
      121 catgcaaacc gaaaatagtg tccggatgga accaagatat acccgacccg gtccgcggcc
      181 taaccgaatc ctgattatca tcagtgggtt tgttgcgctc tacctggtca acctctggat
      241 gagcggagaa ctgtacaccg ttaacctggc gcttaaaaac ccccggctcc aattattcgg
      301 cgtgatcatg accgccatcc tgctggaagg tttcccgttt ctcctgttgg gctcctgcat
      361 ttccggggcg attgaagtcc tggtgccccg cgaagtattg gaaaaacgcc tcttttctgc
      421 caatccgata aaggcggcgg ccgtcggttc gctgctggga atggccttcc cggtatgctc
      481 ctgcggcaac ataccggtaa ccaggcggtt gatgagcaag ggagttccgg tcgcgggcgc
      541 ggtcagttac ctgctggcgg cgccgatcat caatcccatc acgctgtttt caacgatcgc
      601 ggcctttccc caagaaaaga cggtcgttat cggaagactg accctggcct tcatcatcgc
      661 caacatagcg gggttgtgcc tgggcaggtg gaagcccggg gaaattctca aggccgaggc
      721 cggccacgat ctctgcccgc acgaacatca ccaccatgat cattcgggaa atcggctcgg
      781 ccgcatccta tcccacgccg agtccgactt tttcctgacc ggacgctacc tgatcatggg
      841 cgcctccgtc gcggcgcttt tccagacctt cattccccgg accgcggtca acgcgctcgc
      901 cgccaactcc ctgctttcgg tgttcgtaat ggaattaatg ggcatcgcct tttccctctg
      961 ctccttcgcg gacgcgtttg tggccagcac cttcaccgct ctgccggcgg ccgccaagac
     1021 cggattcatg gtcgccggtc cgatgaccag tctggcgctg gtcaccctct acctgggtac
     1081 cttccagcgg aaattcgcgg gacggctcat catgatcacg gccgccggcg tgttaatcct
     1141 ggcctcggtc tgggcgcttc tttcaatcgg aggataaaaa ttgaacaagc aatggtggct
     1201 gcgcgccggg gttatcctga cctggttcac cttcatcgtc atcattctgg cgggcggata
     1261 cctgccgatg tatatccgca agagttatgc tccgctggcg ttcgtcgccc tggtcatcct
     1321 ggccttgatg gcaatccaac cggaaacttc aggcgccggc cacggccatc aagagtcaat
     1381 ttcccggtcc tccctcctca ttttcctgct gcccgttctc ttcgtcgccg tcgtacgccc
     1441 gggcaccctt tcctccctgg cggttcagac ccggggcatc agcaccgcct tggccggaac
     1501 cgacgcctcg ctcgcggaaa gcctggccgc gcgggtcgca accgaaagcg ggtatcggag
     1561 catgaacctg aagcagctgt tgaccctggt gtccggcagc cccgaaaagg ccgacgcgct
     1621 gccgatcgcg gtcgaaggaa tggtatttaa aaattccagt cacgctccgg gaaccttcat
     1681 gctggtccgc tttctcatca cctgctgcgc ggcggacgcc acccctctcg gaatcgcggt
     1741 atcctgggac gcggcctccg gaccgccccc tgaaaacgcc tgggtgctcg tgcgcggcaa
     1801 aatttccttt tcggaagcga ctccaagcgt catcgccgat gagatacagg taatgccgaa
     1861 tcccgccaac ccttatcttt attgaagaac gaaactcgtt tttgcggtca cccggcgtca
     1921 ggggcaatcg caaggaagca tatcattgaa aaatcccggc cgggttgttc gcagccacgg
     1981 ccggccgata tccccaggga tcattcgtcc ggatatggcg ggtggaccgg gaaaaaagga
     2041 gagggatgac catagccatc gccagcggca agggcggcac cggcaagaca accgtagcgg
     2101 tcaacctggc gcggatcttc gaaaccgaag tgcagctgct ggactgcgat gtcgaggcgc
     2161 ccaacgccca cctctttctt aaaaccggga accgtaagga ggcggccgtc gccatccggg
     2221 tgccggaggt tgacgaaaag atctgcgacg gctgcggcga atgcggccgc ttctgcgcct
     2281 acaacgccat cgccgtcttc ggccccaggc cgatggtctt tccggagatg tgccacggct
     2341 gcggcggctg cgccctggtc tgtcccaaaa aggccatccg cgagacggac cggcgcatcg
     2401 gcgtcatcga aacctcacgg accgggaaca tcaccctcat ccagggctgc ctggatatcg
     2461 gcgtggccat ggcgccgacc ctgatccggg ccgtcaaggc gcgccggcag accgaccggc
     2521 ccgccatcct ggacgcgccg ccgggaacct cctgcccggt gattaccacc ctgcggaagg
     2581 ccgatttcgc gctcctggtc accgaaccga ccccatttgg cctctacgat cttacaatgg
     2641 ccgtggagat gatccgggaa ctcaaaatac ccttcggggt gatcgtcaac cgggtcgggg
     2701 tgggcgacga ccgggttcac gccttctgcc gggagaccgg gatcccgatc ctgctcgaga
     2761 ttccggacga ccggcgtatc gccgaggcct actcgcgcgg agaaatcctg gtcgaggccc
     2821 ggccggagta tcgcgccctc ttcaccggcc tgctcgaaaa gataaaaaag gcaggcggaa
     2881 ataacaggct gaaggagggg aaagaatgaa actcggcatc gtcatcaccc ggaccgaccc
     2941 ggagagcgtc ttcaacgtcc tgcgcctggc cctttacagc ctggagcatg gcgaccgggt
     3001 gcgtatcttc ctctcgggcc ggggcgtgga gattgaccgg atcgaggatc cgaagttcga
     3061 cgtcaagggc ctggcgcaga aggtcctgaa cgccggcggc agtttccacg cctgcggatc
     3121 ctgtctgaaa ctgcgcaatt cgaacggctc tgatatctgt cccctttcga ccctcaagga
     3181 ccactacgag atcgtgcgcg actcggaccg gctcgtaacc atatgaaaga gggaaaaacg
     3241 aaaaccaggg aactggtcgt cgtcagcggc aagggcggca ccggcaagac cagcctgacg
     3301 gccgccttcg ccgcgctggc cgaagcgccg gtggtcgccg actgcgacgt ggacgccacc
     3361 gacctgcacc tgctgctttc gccccggatc ctggaacgcc accggttccg cagcggccac
     3421 gaggccgtca tccgccagga ggattgcgtc ggctgcggcg gctgcctggc ctattgccgc
     3481 tatgacgcgg taaagagaac gatccagccc gccggcgacg cagtcttctc catcgacccg
     3541 gtcgcctgcg agggctgcgg ggtctgcgtc cgtttctgcc cggagaacgc catcgatttc
     3601 ccggaacggg agtgcggcga atggatgata tcggagtccc gctgcggccc gatggtgcac
     3661 gccaagctgg gcgtcgcggc cgaaaattcc gggaaactgg tctccatcgt ccgccgggag
     3721 gcccggcgcc tggccgatgc ggaaaaccgg cggctgatca tcgtggacgg cccgccgggc
     3781 atcggctgcc cggtgatcgc ctcgatgacc ggcgccacgc tggtgctggc cgtcaccgaa
     3841 ccgaccctct ccggcgaaca cgacctgaag cgggttctct ccctggccgc ccacttcagg
     3901 ataccggccg ccgtctgcgt caacaagtgg gacctcaatc ccgacctggc cggccggatt
     3961 gaaaccgggg cggcccgggc gggcgcgcgg atcgtcggcc gcgtccggta cgaccgaagc
     4021 gtaaccctgg cccagatgaa aaaactgacg gtggtcgaga ccgaagcccc ctgcgctgaa
     4081 gatgtccgct ccgtctggca aaacctcaac ctggagaagg catgaagaat aacgagttca
     4141 acgaccaggt ccagctgcag tcccggctcg agcgcattgg ccacaagatc atcatcctct
     4201 ccggcaaggg cggcgtcggc aagagcacca tctcggtcaa cctggccgtg tccctgatgc
     4261 tggaaggcaa gcgggtcggc ctgctggacg tcgatatcca cggcccaagc atcccgacca
     4321 tgctcggcct ggaaaaagaa atcctccggg cggacgaaga cggcctgttc ccgatcgaac
     4381 tgggcgacct gaaggtcatg tcaatcggct tcctgctgaa gaaccaggac gaggcggtca
     4441 tctggcgggg gccgatgaag atgggggcca tcaagcagtt cctccaggac gtggcctggg
     4501 gcgacctcga ttacctgatc atagactcgc cgcccggcac cggcgacgaa cctctctcgg
     4561 tctgccagct gatcggcggc ctggacggcg cggtggtggt caccacgccc cagaaggtgg
     4621 ccgcggtcaa cgtgcgcaag tcagtcaact tctgccgcca gctgaaggtg ccggtgatag
     4681 gcatcgtgga gaacatgagc ggcttcgcct gcccgaaatg cggccaggta acgccgatcc
     4741 tgcgcgaagg cggcggccgg cgcatcgccg ccgacatgaa gacgcccttc ctgggcgcca
     4801 tccccatcga tccggagatc gccctttccg gcgacgaagg ccggcccttc atccaccact
     4861 atgccgcctc gcccaccgcg ggcatcatgc gcgaaatcat caagcccatc ctggccctgg
     4921 accggaccgg ccacccccgg ccgaacggaa aaaccacttc gccaaccgag gagaaaaaat
     4981 gaagatcgcc attccgctgg cggccggaag actctcgatg catttcggcc actgcgaaca
     5041 attcgcgctc ctggacgttg accctgagaa gaagagcata ctcaaccggg cggatgtcac
     5101 tccgccgccg caccagcccg gactcctgcc gccctggctg gccgaacagg gcgtcaccct
     5161 ggtcatagcc ggcggcatgg gccagcgcgc ccaggacctc ttcgcccggc agggcatcaa
     5221 ggtcgtaatc ggcgcgccgg ccgagagccc cgagaaactg gccgccgact tcctctccgg
     5281 gaacctgaaa gagggagaaa acgtttgcga tcattgagcg aagcaccgct gacccttagt
     5341 gtgttcttgg tgcgtcgtgc tcgcgagcgg tagatggaga ttcataaaaa caagtcacac
     5401 ccctaataga gaagcctttc gaaaacatga cgaacgttca tttgtttgtc attccctggt
     5461 gcagcagaaa aaaatccagc agttcctgcc gttatttttt ggactttccg gcaggagacc
     5521 ggccttttcc cttttcaccc gggtggggta tactactcgt taaaagcgcc ggggagtcga
     5581 aacgctgaag ggggagaaga tggccgcaag cgtcaggatc accatcctgg tggacaacca
     5641 ggccggggag gggctggccg ccgagcacgg cttcgccctc tggatcgaaa ccggcgggaa
     5701 aaacatcctc ttcgacactg gcgaggggcc ggccctgctg cacaacgccc cgcagctggg
     5761 cgttgacctg gccacggccg attgcctggt actgagccac ggccaccacg accataccgg
     5821 cggcgtcgcc gccctgctgg agaagaccgg ccccctcgac gtttacttcc atcccggcct
     5881 gaccgatgaa cggtacgcca tccgcagcgg caaggccatc tccatcaaga tgccgccggc
     5941 ctcggcggcg gcccttgcaa acctgccgga ggccaggaag cacccggtcc cggccccggc
     6001 cgagattttc gaaggcgtcg gcctgaccgg ccccatcccg cgccggaccg cctacgagga
     6061 taccggcggc cccttcttcc tcgacccggc ggccggccgc gccgacccga tcgaggacga
     6121 actggccctc tggatcagga cctccggcgg actggtggtc tgcctgggct gcggccacgc
     6181 cggcctggtc aataccctca atcatgtccg ccggctgaac ggtgaagaga aaatccgcgc
     6241 cgccatcggt ggctttcacc tggttgaatc cgaggagcgg cgtttcgacc tgaccggggc
     6301 ggccctgacc ggcctggcgc cggaactggt cattccctgc cactgcaccg gcgaccgggc
     6361 cgtcgaggcc ctgaaatcaa ggctcgggaa acgggtcatc accggccgct ccgggaactc
     6421 ctttgaattt tgaagatttc tctttttgct gtcattccca ggtgcccatc cgcctgcgcg
     6481 catccccaaa agtagtagcc agggatccat aaatgcctgt cattccctca tgtagtagga
     6541 gggaatccag cagttgtctt tattcagttg tttttcagtt taaaaccttt taagtactgg
     6601 atgcccgtct acgacacacg ggcatgacga acgttttgga taccaaacca aaagcgtaga
     6661 ggaaaaccat gatcaccccg cgcgagaaca tgctgaagat attccgccac gagcggccgg
     6721 actggctgcc gctcaccggc cactgcgacc cctataacca gcccagccgg gaaggcatgg
     6781 acccggccct ggcttcggcc ctcggcgagg taaaatgggg cgacgaatcc atcgtgacct
     6841 tttcgcgcta cctcggcctg gacatcctcg actggttcgg cctgccgtcg gtgcgcagct
     6901 cccggcgccg ggtaacgatg gaggagatcg tcgagggcga cgtcaagacg catgtctggc
     6961 acactcccag gggcgacctg cgcgaaacca tccgggtcct ccgcgacccc agcggcgccc
     7021 ggtcttcgaa ctacaccgaa cacctggtca agagtccggc cgacctgccg gccctggccg
     7081 aaatattcga ggacgagctc atcgagtccg accccgacgg cttcgagaaa acccgcc
//
LOCUS       DAOQYN010000094         7043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_11068_length_7043_cov_4.891099, whole genome shotgun sequence.
ACCESSION   DAOQYN010000094 DAOQYN010000000
VERSION     DAOQYN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11068_length_7043_cov_4.891099"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..811
                     /locus_tag="PKN80_04915"
     CDS             101..811
                     /locus_tag="PKN80_04915"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ35390.1"
                     /translation="MGRLNVKDDLLILIPAYNEAAFITGVVAAVRPLAARVLVVDDGS
                     GDRTAELAAAAGAEVVRHPVNRGKGRALQTGFRYALDKGYRVVLVMDGDGQHHPEDIP
                     GLLERFQRGGVGVVVGCRPRNLANMPLDRFLTNWFTSALVSLLAGQCIRDSQCGFRVF
                     DSRVFPALSFSTGRFDTESEVLVEASRAGFRIASAPVRTIYGQEKSKINPLVDTLRFF
                     RFCLRMLGRICLGRACPR"
     gene            808..1470
                     /locus_tag="PKN80_04920"
     CDS             808..1470
                     /locus_tag="PKN80_04920"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNQ35391.1"
                     /translation="MKTVDFEAARRLMVERDLAGRGIRSLPLLEAFRSVPREIFVPEN
                     YRDRAYADCPLPIGEGQTISQPYMAALMIEALDLKASDRVLEVGSGSGYVVALLARLV
                     SRVYGIERHPSLASESAVRLERLGVSNAEIRAGDGTRGWPEAAPFQAILVSAATGRPP
                     RALLEELDEGGRLAVPVGTPELQELQLLRRTAGRIRTEHLGGCRFVPLIGENGWEPES
                     NI"
     gene            1446..1949
                     /locus_tag="PKN80_04925"
     CDS             1446..1949
                     /locus_tag="PKN80_04925"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNQ35392.1"
                     /translation="MGAGIKYLTTLGISLLPIFELRGAIPWGILKEQLPWTRVVLVAV
                     IGNFLPVIPLFLGIERLAAWLGRFPALARFFGWFFERTRRRSRAVACSEFIGLAVFVG
                     IPLPMTGAWTGAVAASLLRIPFRRAVSAVGLGILMAAAVVTPITLGIRYGWSRLSPEI
                     IRLFIKY"
     gene            1969..2244
                     /locus_tag="PKN80_04930"
     CDS             1969..2244
                     /locus_tag="PKN80_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNQ35393.1"
                     /translation="MKRFRCYFSGRVQGVGFRFYVERAALGFPGLTGFVRNLADGRVE
                     VVAEAEGPVLDAFLEALRGGDLGRNVRRLEVLEEKATGAFADFRITF"
     gene            2406..2801
                     /locus_tag="PKN80_04935"
     CDS             2406..2801
                     /locus_tag="PKN80_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006922414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNQ35394.1"
                     /translation="MDTIEIVTSKPREFLEVTGRITARLAANGWRDGVLHLFVPHTTA
                     GLFVNEHADPDVARDIESLAGELVSDRFPYRHAEGNSPGHVKSVLFGISLALIVEDGR
                     LVLGTWQGVFFAEFDGPRRRKLFLKFMKS"
     gene            2821..3135
                     /locus_tag="PKN80_04940"
     CDS             2821..3135
                     /locus_tag="PKN80_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006621847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ35395.1"
                     /translation="MASRKKNPDLNELITFTPGGILSRVVLEGEHLEATLFCLAAGSG
                     ISEHTAGREAAITVLRGRGVFVLEGRRISMSPGVFISMKPGAPHSLKATEDLAFLLML
                     GS"
     gene            3174..5183
                     /gene="ligA"
                     /locus_tag="PKN80_04945"
     CDS             3174..5183
                     /gene="ligA"
                     /locus_tag="PKN80_04945"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNQ35396.1"
                     /translation="MDKQSAEKRIAALVETINRHNHRYYVEANPVVSDAEYDGLVREL
                     KELEGRFPELQRTDSPTLRVGGQPLDGFRTVEHLRPMLSLENGYSEAEVREFDRRLAR
                     LLETDEIEYVVELKVDGVALSLVYESGRLLRAVSRGDGWRGDDVTANIRTIRTIPLAI
                     PYAGRLEVRGEVYLTRADFARINRERLESEEPVFANPRNATAGSLKLLDSREVARRRL
                     LFFAWAGFPEPELASHWETLEFLGRNFFPVNPNIKLVRGVGALLDACAFWAERRRQLS
                     YETDGLVFKVNRLTVQAAVGSTSKSPRWALAYKFPAEQATTRILDILSQVGRLGTITP
                     VAVLEPVRLAGTTVSRASLHNQDEIRRLGVRIGDRVFIEKAGEIIPQVVKVITELRTG
                     TEREFEFPKFCPACRGPLSRAEQEVAVRCTNPGCPAQVKERILHYAGREAMDIEGLGV
                     ELVDRLVDLGLVKDAAGLYRLTPGQLVNLERLGEKSAGKLLASIQASRERPLARLIHS
                     LGVRHVGLRAAEVLAGRFASLDELAAAPEADLTAIHEIGPVIAGSLADYFRNPDNRRL
                     LEELKAAGVRFDRRDGLAGPAGTGPLAGRTFVITGRLEKWTRSRAREIIQRLGGRVAD
                     SVSGRTDFLLAGADPGSKLDRARELRVRVLTEAEFSKMIDTRQIP"
     gene            5191..5832
                     /locus_tag="PKN80_04950"
     CDS             5191..5832
                     /locus_tag="PKN80_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ35397.1"
                     /translation="MLKIKTLEVGPLQTNCYLVFDPECQEGLIVDPGADKPKIIKTVE
                     KSGVRFKSVVNTHGHFDHVGADTIKELLGVPLYLHQADLPLLKNARVNQALILGVDNG
                     EKIPTPDGFLEEGQELAVGGGRLTVWHTPGHSPGGICLVAAGFVLTGDTLFNSGVGRT
                     DLEGGSDPDLERSLRRLLKLPPETVVYPGHGPSTTIGAERRLLVDPGDKQATV"
     gene            5855..6736
                     /locus_tag="PKN80_04955"
     CDS             5855..6736
                     /locus_tag="PKN80_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HNQ35398.1"
                     /translation="MAETGLPDQESFFGYLTLERGLSRNTILAYRRDLKRFDVFLSGR
                     GLTLESITYPQFTSFLMECRRTLAAASLARLMAAISTYLKFLLQEGVLKAHPLPDLES
                     PRLEKNLPGVLSPAEVERLLAAPKPGRPGLKDRAILELLYATGMRVSELTGLKREDLD
                     LAEQLVRVRGKGGRERLIPFGAPAAAALRSYLETVSGSASAEVPLFRNRQGRALSRVA
                     VWKLLRRSALSAGITAKVYPHILRHSFATHLLTAGASIRLVQELLGHQSLATTQIYTH
                     LDRSRLKEVHRRFHPRP"
     gene            6860..>7043
                     /locus_tag="PKN80_04960"
     CDS             6860..>7043
                     /locus_tag="PKN80_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35399.1"
                     /translation="MDRIAPAAAGDFEEIQRMLESSYNHYRDFFPQQYPTVWRRAAFD
                     PGNTILMRSGGRIVGLV"
BASE COUNT         1110 a         2317 c         2364 g         1252 t
ORIGIN      
        1 tgcttaacac cgcccgggac gtcgccttcc ggctgctgga cggcgatccg ggcttgaccg
       61 gccagccgca gctccgggaa cggctggcca ggcagggttt atgggaaggc tgaacgtaaa
      121 agatgatttg ctgattctga tcccggccta caacgaggcc gcgttcatta ccggggtggt
      181 ggccgcggtc cggccgctgg cggcccgggt cctggtggtt gacgacggtt ccggcgaccg
      241 gacggcggaa ctggccgccg cggccggcgc cgaagtcgtc cgccacccgg tcaaccgggg
      301 caaggggcgg gctctgcaga ccgggttccg ctacgccctg gataagggct accgggtggt
      361 cctggtcatg gacggcgacg ggcagcacca tccggaagac atccccggcc tcctcgagcg
      421 gttccagcgg ggcggggtcg gggtggtggt tggctgccgc ccccggaatc tcgccaacat
      481 gcccctggac cgctttctga ccaactggtt cacctcggcc ctggtctccc tgctggccgg
      541 ccaatgcatc cgggacagcc agtgcggctt ccgggtcttc gattcccggg tctttccggc
      601 cctctccttt tcgaccggcc gtttcgatac cgagtccgag gtgctggtgg aggcgagccg
      661 ggccggattc cggatcgcct cggcgccggt gcgcaccatc tacggccagg agaagagcaa
      721 gatcaacccg ctggtcgaca ccctgcgctt cttccgtttc tgcctgcgga tgttgggccg
      781 catctgcctc ggccgcgcct gtccgagatg aaaacggtgg atttcgaggc cgcccgccgc
      841 ctgatggtgg aacgggacct ggccggccgg ggtatccggt cgctcccgct gctggaggcc
      901 ttccggagcg tgccgcgcga aattttcgtc ccggaaaatt atcgggaccg ggcctatgcc
      961 gactgcccgc tgccgatcgg cgaaggccag accatcagcc agccctacat ggcggccctg
     1021 atgatcgagg cgctcgattt gaaggcctcg gaccgggtcc tggaggtggg ttccggcagc
     1081 ggttacgtgg tggccctcct ggcccggctg gtgtcccgcg tttatggtat cgaacgccac
     1141 ccttcgttgg cctcggaatc ggccgttcgc ctggagcggc tgggcgtaag caatgccgag
     1201 atccgcgccg gcgacggcac ccgcggctgg ccggaggcgg cccccttcca ggccatcctg
     1261 gtcagcgcgg cgaccggccg gccgccgcgg gcgctgctcg aggaactgga cgagggcggc
     1321 cgcctggcgg ttccggtcgg cactccggag ctccaggaat tgcagctgtt gcgccggacg
     1381 gccggccgga tcaggaccga gcacctgggc ggctgccgct tcgtgccttt gatcggagag
     1441 aacggatggg agccggaatc aaatatctga ccactctcgg aataagcctg ctgccgatat
     1501 tcgagttgcg cggcgccatt ccctggggga ttctcaaaga gcaactgccc tggacccggg
     1561 tggtcctggt ggcggtcatc gggaacttcc tgccggtgat tccccttttc ctgggcatcg
     1621 agcggctggc cgcctggctg ggccgtttcc cggccctggc ccggttcttc ggctggttct
     1681 tcgagcgcac ccggcgccgg agccgggcgg tcgcctgttc cgagttcatc ggactggcgg
     1741 tctttgtcgg cattcccctg ccgatgaccg gcgcctggac cggcgcggtg gccgccagcc
     1801 tgctgcgcat ccccttccgg cgggcggttt cggccgtcgg cctgggcatc ctgatggcgg
     1861 cggcggtcgt gaccccgatc accctgggga tccgctacgg gtggagccgc ctctcgccgg
     1921 aaatcatccg gctcttcatc aagtactgac cgccaccggc cgccgaacat gaaacgcttc
     1981 cgttgctact tctccgggcg ggtccagggc gtcgggttcc gcttttacgt cgagcgggcc
     2041 gccctcggtt tcccgggtct gaccggtttc gtccggaacc tggccgacgg ccgggtggag
     2101 gtggtggccg aggccgaggg tccggtgctc gacgccttcc tcgaagccct gcgcggcggc
     2161 gacctggggc gcaacgtgcg gcgcctggag gtgctggagg agaaggcgac cggcgccttt
     2221 gccgacttcc ggatcacttt ttaaaccggg ttctctccgt cggattgacc ggtccggttc
     2281 gagccctcaa gcccctcctg gagcctcttt cccgtgccgg cgccccttga ttgccaaccg
     2341 gtatgggcct gtgctatatt gaaaataaag ggtttcccgg agttccgccg ggaggccgga
     2401 tcagcatgga tacgatcgaa atcgtcacct cgaaaccgcg ggagttcctg gaggttaccg
     2461 gccggatcac cgcccgcctg gccgccaacg gctggcggga cggggttctg cacctcttcg
     2521 tgccccatac cacggccggc ctcttcgtca acgagcacgc cgatccggac gtggcccggg
     2581 acatcgagtc cctggccggg gaactggttt cggaccggtt tccgtatcgg cacgccgagg
     2641 gcaacagtcc cggccacgtg aagtcggtcc tcttcgggat ttcgctggcg ctgatcgtcg
     2701 aagacggccg cctggtcctg gggacctggc agggcgtctt cttcgccgag ttcgacggcc
     2761 cgcgccgccg gaaactcttt ctcaagttca tgaaatcctg aatcgaaccg gaggtttgcc
     2821 atggccagcc gcaagaagaa tccggacctg aacgaactga taaccttcac tcccggcggc
     2881 atcctgagcc gggtggtcct ggagggggaa cacctggagg caaccctctt ctgcctggcc
     2941 gccggcagcg ggatctccga gcataccgcc ggccgggaag cggccatcac cgtgcttcgg
     3001 ggccggggcg tcttcgttct cgaggggcgc cggataagca tgagtccggg cgtcttcatc
     3061 tccatgaagc cgggcgcccc gcactcgctc aaggcgaccg aggacctggc ctttctcctg
     3121 atgctgggaa gttgaagccc gcccggcttc cgggcccgga ttttttgacc accatggaca
     3181 agcagtcggc cgagaaaaga atcgccgccc tggttgaaac gatcaaccgg cacaaccacc
     3241 gttattacgt cgaggccaac cccgtcgtca gcgacgccga gtacgacggc ctggtgcgcg
     3301 aactcaagga actggaaggc cgctttccgg aactgcaaag aaccgattcg ccgacgctga
     3361 gagtgggcgg ccagcccctg gacggtttcc ggacggtcga acacctgcgg ccgatgctca
     3421 gcctggagaa cggctattcg gaagccgagg tccgggaatt cgaccggcgg ctggcccggc
     3481 tcctggagac ggacgagatc gagtacgtgg tggagttgaa ggttgacggg gtggccctgt
     3541 cgctggtcta cgaatccggc cgcctgttgc gggcggtcag ccggggggac ggctggcgcg
     3601 gggacgacgt gaccgccaac atccggacca tcaggacgat cccgctggcc ataccctatg
     3661 ccggccgcct cgaggtccgg ggcgaggttt acctgacccg tgcggatttc gcccggatca
     3721 accgggagcg cctcgaatcg gaggagccgg ttttcgccaa cccgcgcaac gcgacggccg
     3781 gctcgctcaa gctgcttgat tcccgggagg tggcccggcg ccgccttctc ttcttcgcct
     3841 gggccggttt cccggagccg gaactggcga gccactggga gacgctcgaa tttctcggcc
     3901 ggaatttctt tccggtcaac ccgaacatca agctggtccg gggcgtaggg gcgcttctgg
     3961 atgcctgcgc cttctgggcc gagcgccgcc gccagttgtc ctatgaaacc gacggcctgg
     4021 tcttcaaggt gaaccgcctg accgtccagg cggcggtcgg ttcgacctcc aaaagcccgc
     4081 gctgggcgct ggcctacaag tttccggccg agcaggccac cacgcggatc ctggacatcc
     4141 tgtcccaggt ggggcggctg ggaacgatta ctccggtggc cgtgctggag ccggtccggc
     4201 tggccggcac cacggtcagt cgggcctcgc tgcacaacca ggacgaaatc cggcggctcg
     4261 gcgtccggat cggcgaccgg gtcttcatcg aaaaggccgg cgagatcata ccgcaggtgg
     4321 tcaaggtcat taccgaactc cggacgggaa ccgaacggga gttcgaattt ccgaagttct
     4381 gcccggcctg ccgggggccc ctttcgcggg cggagcagga agtggcggtg cgctgtacca
     4441 acccgggctg tccggcccag gtcaaggagc ggatcctgca ttacgccggc cgggaggcca
     4501 tggacatcga gggactgggc gtggaactgg tcgaccgcct ggtggacctg ggactggtaa
     4561 aggatgccgc cggcctctac cggctgaccc ccggccagct ggttaacctg gaacggctgg
     4621 gtgaaaaatc ggccgggaag ctgctggctt ccatccaggc cagccgggag cggccgctcg
     4681 cccgcctgat tcacagcctt ggtgtccgtc acgtcggact ccgggcggcc gaggtcctgg
     4741 ccggacggtt tgcctctctg gatgagctgg cggccgcccc ggaggcggat ctgaccgcca
     4801 tccacgaaat cgggccggtg atcgccggca gcctggcgga ctacttccgc aatccggaca
     4861 accggcggtt gctggaggaa ttgaaggcgg ccggtgtccg ctttgaccgc cgggacggcc
     4921 tcgccggtcc ggccgggacc ggaccgctgg ccggccggac cttcgtaatt accgggcggc
     4981 tggaaaagtg gacccggtcc cgggcccggg agatcatcca gcgcctgggc ggccgggtcg
     5041 ccgactcggt ctccggccga actgatttcc tgttggccgg ggccgatccc gggtccaaac
     5101 tggaccgggc cagggaactc cgggtccggg tcctcaccga ggccgaattt tcgaagatga
     5161 tcgacaccag gcaaatacct taggggaacg atgctcaaga tcaaaacgct ggaagtggga
     5221 ccgcttcaga ccaactgcta tctggtcttc gacccggaat gccaggaagg tctgatcgtc
     5281 gatccggggg cggataagcc caaaataatc aagacggtcg agaaatcggg tgtccgtttc
     5341 aaatcggtag tcaacaccca cggccatttc gaccatgtcg gcgccgacac catcaaggag
     5401 ctcctgggcg tgccgcttta cctgcaccag gccgaccttc ccctgttgaa gaatgcccgg
     5461 gttaaccagg ctctgatact gggggttgac aacggcgaaa agattccgac gccggacggc
     5521 ttcctggagg agggacagga actggcggtg ggcggcggcc gccttaccgt ctggcatacg
     5581 cccggacact ctcccggcgg catctgcctg gtggcggccg gtttcgtcct gaccggcgac
     5641 accctcttca acagcggcgt cggccggacc gatctggagg gtggttcgga tccggacctg
     5701 gaaaggtcgc tgcgccgact gctgaaactt cccccggaaa cggtcgttta tccgggccac
     5761 ggcccgtcca ccaccatcgg ggccgaaagg cgcctgctgg tcgatccggg cgataaacag
     5821 gcaaccgttt gaatccggac ggaccggagg gaccatggcg gaaacagggc tcccggacca
     5881 ggaaagcttc ttcggctacc tgaccctcga acggggtttg agccggaaca ccatcctggc
     5941 ttaccggcgc gacctgaaaa ggtttgacgt ctttctctcc ggccgcggcc tgaccctgga
     6001 atcgattacc tacccccagt tcacctcctt cctgatggag tgccggcgca ctctggcggc
     6061 cgcctcgctg gcccggctaa tggcggccat ctccacctac ctgaaattcc tgctccagga
     6121 aggggtcctg aaggcccacc cgctgccgga cctggagagt ccccggctgg aaaagaacct
     6181 gccgggcgtc ctttccccgg ctgaggtgga acggctgctg gccgctccga agccgggtcg
     6241 acccggcttg aaggaccggg ccatcctgga gctcctttac gccaccggca tgcgggtgag
     6301 cgaattgacc ggcctgaaac gggaggacct ggacctggcc gagcagctgg tccgggtccg
     6361 gggcaagggc ggccgggaac ggctgattcc cttcggcgcg ccggcggcgg cggcgttgcg
     6421 gtcatacctg gagacggtgt ccggttccgc ctcggccgag gtgccgctct ttcgtaaccg
     6481 gcagggccgg gccctgagcc gggtggcggt ctggaagctg ctgcgccgca gcgccctttc
     6541 cgccgggatt accgccaagg tctatccgca tatactgcgc cactcgtttg ccacccacct
     6601 gctgacggcc ggggccagca tccggctcgt ccaggaactg ctcggccacc agagcctggc
     6661 caccacccag atttacaccc atctggaccg ctcccgtctc aaggaagtgc accggcgttt
     6721 tcatccccgt ccctgagccg ccgccggttg cgaaataatc ggccaggggt tatactaaga
     6781 cataactttt tggcctgttt ttatctgtgg ctgcgccggc cggggcgttc ccggcacgat
     6841 caaatccgaa gctcggcaaa tggacaggat agcgccggcg gccgccggcg attttgaaga
     6901 gatccagcgg atgctggagt cctcctacaa tcattaccgg gattttttcc cccagcagta
     6961 cccgaccgtc tggagacggg ccgcttttga cccggggaat acgatcctga tgcgttccgg
     7021 cggccgcatc gtcggcctgg tgc
//
LOCUS       DAOQYN010000095         6927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_11311_length_6927_cov_4.936409, whole genome shotgun sequence.
ACCESSION   DAOQYN010000095 DAOQYN010000000
VERSION     DAOQYN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11311_length_6927_cov_4.936409"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>6927
                     /locus_tag="PKN80_04965"
     CDS             <1..>6927
                     /locus_tag="PKN80_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNQ35400.1"
                     /translation="YRLAGLNEGLYRIRFDQVNAGYLARWYNDRSDQAAADSIPVTPP
                     DETPGIDAVLRYPRVTAEVAAGNGTVFPASQDIFYGQPAAVTAVAADGFSYQKYTLDG
                     TDHTPGDSTLEINQVMADSHVTVTFIPVADLRVKRAEAPAEIWTGSAFDLTWELENRG
                     LADIGAVWTDYIYLSSDDQVGNDTPLVGFNYNAGLNAGASVTRIQTITIPRNLLKSEG
                     SVYLLVVTDFHNNVYEGQSFEAAENNNRRFTAQPVQVRVSPMPDLEVSMVNPPSSSFS
                     GQNATFSFTIKNTGNGPTDAALWYDAAYLSLDTQFQPNIDTLLNNPRNGSYLAPEDTY
                     TTSFEARLPNGLEGSYYLLVAADAYNWVGESLEDNNLNYQAFPITLTTPPNLKVISVT
                     APPVAASGQPVEVSWQVKNDGNGPINSSWWSDQVFLSEDTSLDTGDHYLGNFYKSGSL
                     ASKESYEASLTVTLPAGVSGPRYFLVWTDRNNYIYEHASEDDNVGATDQPSNITLVAP
                     DLEVLEVAAPGSATASRNLTVRFTVVNNGTAATSNNSWTDAVYLSEDTGLDTGDLCLG
                     EKTHFYALTESDTYLAELTVTLPDAISGTRYLLVWTDNRRQVVELDDLNNVWASGPVN
                     VLSRPADLVISNVTAPAASDSGRAVTVAWKVTNSGTGDTVASNWADRVYLSRDTQLDT
                     ALDIQLGSYVHEGRLDIGETYQRSEPVELPIALDGDYYLFVLADASGSVYEGSSEANN
                     RSEPLPIRVTRVIPDLQALLLNAPAEARSGDAIALDWTVRNNGNGRTDADLWRDAIYL
                     SADASLDTEIDTRLGLVHHTGRIDPGASYNGSGTVSLPPDVSGTCYLFLFANSDAAVT
                     EIDRGNNGRRAEAATIITLTPAPDLAVTAVSPPASILSGQPFTLNWTVANQDASIDTS
                     LSWIDSAWLSRDQVLDNTDLYLGSFSRNALGLAGGTSYTGELRATVPMGLTGPYYIFV
                     RTDISNRVYERGRKANNLGYNTPAVQVDLPAPADFQPLAEMTFPDTGIAGQTVNIGYR
                     VQNQGVNAFQGSWTDALYLSSDDQWSSDDLLLGRISKNTNLAPGQEYAGALNTVLPGV
                     VPGYYHVIASTDIYNNVLESEEANNRTASAGRIYVDAELLELDRPDTGILGYGQSVYY
                     RLNVPVAGETMLLSFDSVSESNPNELYLSFGRMPNRGSFDFAYDRPFSPDQEVVVPLT
                     RDGDYYVLAYNSGGWQNARYTILARLIPFSLRAVDPAEAGNAGPSTLKISGARFTRDT
                     AFRLIDPAGAPLDPTRTLVKDSTIAYATFDLTGRNIGRYRLLALKAGETAEFDSGLVV
                     NPGLGPNLQAEINGPSPIRPSRDYIFYVDYANTGDGDAAAPILLVESTTNTAFGFNIN
                     ELVGGQVLQILGSGSADLGGIIRPGEGFGFPMYFRSPDTIQPVEFRVWIITVDDTRPI
                     DWGLVEQLIRPAGIPEAEWQLVWSRIKDRIGTTWGDYVRAMGEMANLLSGRGEDASDV
                     RLLFAELFNQVSGYPTSRLSGRVINGETGQLMAGATVVARLELTDQAQPDTYISHLAA
                     TNAAGEFLFTNLPVGTYGFHVENYRVDPAASDTMIAVGYDTDALGLVLYAYPIPADTL
                     SPPAPDDDQEARLTLGSDGTPHLVWRRGKALWHAYRDGSGWTGAPLVDAATGDTLTGY
                     ETRLTAAANLFEGTPRTPGLFLTWRRGEGNQAELAYATGRPRAGGGFEWSAQATVTAD
                     SILDQHPALVVTNDGRVLLVFLKSDAAVSDTATRVRDDADLYFERINLDPAGLSWPAL
                     VRVPLGTAADGPGTVYVPLADAQTFSFAMGIQKETADPLPSIIPIIGGKRCKFWVDGQ
                     VTGGATCKAIAAGQLQGEVQLFERVTGALAASVSAEWGSNCDQKAYVFNKASINLTAQ
                     ATGEIPAFYYMVPGDGWFVDKLFELEAGATVTGSISGSAGWEAANFPGLPNTGEVAVS
                     VGLGPYGKAKALDGTLEGTLKGVGSVSIRLLPTCELTGSSLTFAIDLTAGEGRTHWDK
                     TWSWERKPDSPWYRPWSLDRPALSPEMARAAMSLGGRPGLQVIEDDYGILTISTGAPV
                     FSWEEWTGTGASHGDNSVLPAGELAADLYEDGPPALARSEPGEIRLAWTRDVPPGSQD
                     GSQVMVALYRNEDNRFLTAESIPGSLGYNKDLNLVYDNRGLPLALWAQVDSSGLGPAT
                     SAEDFYARLNTGQIKYSVYQGGVWSSPQSGPLALAGNNSRPTLGRTHDNRVIAAWINR
                     AGGGPARLMAAFWDGNAWSSPVQLAEGNLAGTPAAGTLGDRTALFWTQDADPSETASW
                     FRI"
BASE COUNT         1205 a         2658 c         2057 g         1007 t
ORIGIN      
        1 taccggctgg ccggcctcaa cgagggcctc taccggatcc gtttcgacca ggtcaacgcc
       61 ggctacctgg cgcgctggta caacgaccgg agcgaccagg ccgccgccga cagcatcccg
      121 gtcaccccgc cggacgagac gccggggatc gatgccgtcc tgcgttatcc ccgggtgacc
      181 gccgaagtgg ccgccggcaa cggcacggtc ttcccggcca gccaggatat cttctacggc
      241 cagccggccg cggtcaccgc ggtcgccgcc gacggtttct cctatcagaa atacaccctg
      301 gacggcaccg accacacccc gggtgattcc accctcgaga tcaaccaggt catggccgac
      361 agccatgtca cggtaacctt cataccggtg gccgatctcc gggtcaagcg cgccgaagcg
      421 ccggccgaga tctggaccgg cagcgccttc gatctgacct gggagctgga aaaccggggc
      481 ctggccgata tcggcgctgt ctggaccgat tacatctacc tctccagcga cgatcaggtc
      541 ggcaacgaca ccccgctggt cggcttcaac tacaatgccg gcctgaacgc cggcgccagc
      601 gtcacccgca tccagaccat caccatcccg cgcaacctgc tgaagagcga gggcagcgtc
      661 tacctgctgg tcgtgaccga tttccacaac aacgtctacg aggggcagag cttcgaggcg
      721 gccgagaata acaaccgccg cttcaccgcc cagccggtcc aggtccgggt gagcccgatg
      781 cccgacctgg aagtttccat ggtcaacccg ccctccagtt ccttctccgg tcagaacgcc
      841 accttctcct tcaccatcaa gaacaccggt aacggcccca ccgacgccgc gctctggtac
      901 gacgccgcct acctctcgct ggacacccag ttccagccca acatcgacac tctgctcaac
      961 aacccgcgca acggcagtta cctcgcgccg gaggatacct acaccacctc ctttgaggcc
     1021 cggctgccca acggactgga aggcagctat tacctgctgg tggcggccga cgcctataac
     1081 tgggtgggcg aaagcctgga ggataacaac ctgaattacc aggccttccc gatcacgctg
     1141 accactccgc ccaacttgaa ggtgatctcg gtcaccgcgc cgccggtggc cgcctcgggc
     1201 cagccggtcg aggtgagctg gcaggtgaag aacgacggca acggcccgat caattccagc
     1261 tggtggtccg accaggtctt cctctccgag gacaccagcc tggacaccgg cgaccactac
     1321 ctgggcaact tctacaagag cggatcgctc gcctccaagg agagttacga ggcctcgctc
     1381 acggtcaccc tgccggccgg agtcagcggc ccccgctact tcctggtctg gaccgaccgc
     1441 aacaactata tctacgaaca cgcctccgag gacgacaacg tcggggccac cgaccaaccc
     1501 tccaacatca ccctggtcgc ccccgacctg gaggtgctgg aggtcgccgc cccgggcagc
     1561 gccaccgcca gccgcaacct gaccgtccgt ttcacggtgg tcaataacgg caccgccgcc
     1621 acgtccaaca acagctggac cgacgcggtc tatctctccg aggacaccgg cctggatacc
     1681 ggcgacctct gcctgggcga gaagacccac ttctacgccc tgaccgaaag cgacacctac
     1741 ctggccgagc tgaccgtcac cctgcccgac gccatcagcg gcacccgcta cctgctggtc
     1801 tggaccgaca accggcgcca ggtggtggag ctggacgacc tgaacaatgt ctgggccagc
     1861 ggcccggtca acgttctctc ccggccggcc gacctggtca tctccaacgt caccgccccg
     1921 gccgccagcg attccggccg ggcggtcacc gtggcctgga aggtgaccaa cagcggcacc
     1981 ggcgacacgg tggcctccaa ctgggccgac cgagtctacc tctcccggga cacccagctg
     2041 gacaccgcgc tggacatcca gctcggctcc tacgtccacg agggacggct ggatatcggg
     2101 gaaacctacc agcgcagcga gccggtcgaa ctgccgatcg ccctggacgg cgactattac
     2161 ctcttcgtgc tcgccgacgc cagcggctcg gtttacgagg gaagcagcga agccaacaac
     2221 cgctccgaac cgctgcccat ccgcgttacc cgggtcatcc ccgacctgca ggcgctgctg
     2281 ctcaacgcgc cggccgaggc ccgttccggg gacgcgattg cgctcgactg gacggtccgg
     2341 aacaacggca acggccgaac cgacgccgac ctctggcgcg acgcgatcta cctctccgcg
     2401 gacgccagtc tggacaccga aatcgatacc cggctgggcc tggtccacca caccggccgg
     2461 atcgatccgg gcgcctctta caacggcagc ggcacggtct ccctgccgcc cgatgtctcc
     2521 ggcacctgct acctcttcct cttcgccaac tccgacgccg cggtcaccga aatagaccgg
     2581 ggcaacaacg gccgccgggc cgaggcggcc accatcatca ccctgacgcc ggcgcccgac
     2641 ctggccgtga ccgcggtttc gccgccggcc tcgatcttaa gcggccagcc cttcaccctg
     2701 aactggacgg tcgccaacca ggatgcctcg atcgacacct cgctcagctg gatcgacagc
     2761 gcctggcttt cccgcgacca ggtcctggac aacaccgacc tctacctggg ctccttctcc
     2821 cgcaacgccc tcggcctggc tggcggcacc agctacaccg gcgagctgcg ggccaccgtg
     2881 ccgatggggc tgaccggtcc ctactacatc ttcgtccgga ccgacatcag caaccgcgtc
     2941 tacgaacggg ggcgcaaggc caacaacctc ggctacaaca ccccggccgt ccaggttgac
     3001 ctgccggcgc cggccgactt ccaacccctg gccgagatga ccttccccga caccggcatc
     3061 gccggccaga ccgtcaacat cgggtaccgg gtccaaaacc agggcgtcaa cgccttccag
     3121 ggctcctgga ccgacgccct ttacctttcc agcgacgacc agtggagcag cgacgacctg
     3181 ctgctgggcc ggatcagcaa aaacaccaac ctcgctccgg gccaggagta cgccggtgcg
     3241 ctcaacaccg tcctgcccgg cgtcgtcccc ggctactacc acgtcatcgc cagcaccgac
     3301 atctacaaca acgtgctcga aagcgaggag gccaacaacc ggaccgcctc ggccggccgg
     3361 atttacgtgg acgccgaact cctggagctg gaccggcccg acaccggaat cctgggctat
     3421 ggacagtcgg tctactaccg gctcaacgtc cccgtcgccg gcgagaccat gctgcttagc
     3481 tttgacagcg tctcggagag caaccccaac gaactttacc tgagtttcgg ccggatgccg
     3541 aaccggggct ccttcgactt cgcctacgac cgtcccttct ctcccgacca ggaggtcgtg
     3601 gtcccgctca cccgcgacgg cgactactac gtcctggctt acaacagcgg cggctggcag
     3661 aatgcccgct acaccatcct ggcccggctc atccccttca gcctgcgcgc cgtggacccg
     3721 gccgaagccg gcaacgccgg cccctcgacg ctgaagatct cgggggcgcg tttcacccgc
     3781 gacaccgcct tccggctgat cgatccggcc ggcgcgccgc tcgatccgac ccggaccctg
     3841 gtcaaggact cgacgatcgc ctacgccacc ttcgacctga ccggccggaa tatcggccgc
     3901 taccggctcc tggcgctcaa ggccggcgag acggcggaat ttgacagtgg cctggtcgtc
     3961 aacccgggcc tgggtccgaa cctgcaggct gagatcaacg gtccctcgcc catccgaccg
     4021 agccgcgatt atatcttcta cgtggattac gccaacaccg gcgacggcga cgccgcggcg
     4081 ccgatcctcc tggtcgaaag caccaccaac accgccttcg gcttcaacat caacgagctg
     4141 gtcggcggcc aggtcctcca gatcctgggc agcggctccg cggacctggg cggcatcatc
     4201 cgaccgggtg agggcttcgg attcccgatg tacttccggt cgcccgatac catccagccg
     4261 gtcgaattcc gggtctggat catcaccgtc gacgacaccc ggccgatcga ctggggcctg
     4321 gtcgaacagc tcatccggcc ggccggcatc cccgaggccg aatggcagct ggtctggagc
     4381 cggatcaagg accggatcgg caccacctgg ggcgactatg tccgggccat gggcgaaatg
     4441 gccaacctgc tctccggccg gggtgaggac gcctccgatg tccggctgct cttcgccgaa
     4501 ctcttcaacc aggtctccgg ctacccgacc tcccgcctgt ccggccgggt catcaacggc
     4561 gaaaccggcc agctgatggc cggcgccacg gtcgtcgccc gcctggagct gaccgaccag
     4621 gcgcagcccg acacctatat ttcccacctg gccgccacca acgccgccgg cgagttcctc
     4681 ttcaccaacc tgccggtggg cacctacggc ttccacgtcg agaattaccg ggtcgacccg
     4741 gcggccagcg acaccatgat cgcggtcggt tacgacaccg acgcgctcgg gctggtcctc
     4801 tacgcctacc cgatcccggc cgacaccctg agcccgccgg ccccggatga cgaccaggag
     4861 gcccggctga ccctcggctc cgacggcacg ccgcacctgg tctggcgccg cggcaaagcg
     4921 ctctggcacg cctaccggga cggctccggc tggaccggcg cgcccctggt cgacgcggcc
     4981 accggcgaca ccctgaccgg ctacgagacc cggctcaccg cagccgccaa cctcttcgag
     5041 ggcaccccgc gcacgccggg cctcttcctg acctggcgcc ggggcgaagg caaccaggcc
     5101 gagctcgcct acgcgaccgg ccggcccagg gccggcggcg gtttcgaatg gtcggcccag
     5161 gcaacggtca ccgccgacag catactggat cagcacccag ccctggtggt caccaacgac
     5221 gggcgggtgc tgctggtctt cctcaaatcc gacgccgccg tctcggatac cgccacccgg
     5281 gtccgcgacg acgccgacct ctatttcgaa cggatcaacc tggacccggc cggactcagc
     5341 tggccggccc tggtccgggt gccgctgggc accgcggccg acggtccggg cacggtctac
     5401 gtcccgctgg ccgacgccca gaccttctcc ttcgccatgg gcatccagaa ggagaccgcc
     5461 gacccgctgc cctcgatcat cccgatcatc ggcggcaagc gctgcaagtt ctgggtggac
     5521 ggccaggtca ccggcggcgc cacctgcaag gccatcgccg ccggccagct ccagggcgaa
     5581 gtccagctct tcgagcgggt gaccggcgcc ctggccgcct cggtctcggc cgagtggggg
     5641 tccaactgcg accagaaggc ctacgtcttc aacaaggcct ccatcaacct gaccgcccag
     5701 gccaccggcg agatcccggc cttctactac atggtgcccg gcgacggctg gttcgttgac
     5761 aagctcttcg aactggaggc cggcgccacc gtgaccggca gcatctccgg ctcggccggc
     5821 tgggaggcgg ccaacttccc cggcctgccc aacaccggcg aggtggccgt ctcggttggc
     5881 ctcggtccct acggcaaggc caaggccctc gacggcaccc tcgaaggcac cctcaagggc
     5941 gtcggctcgg tctcgatccg gctgctgccc acctgcgaat tgaccggctc ctcactcacc
     6001 ttcgccattg acctgaccgc cggcgaaggg cgcacccact gggacaagac ctggtcctgg
     6061 gaacggaagc ccgactcccc ctggtaccgg ccctggtcgc tggaccggcc ggcgctctcg
     6121 cctgaaatgg cccgggcggc catgtccctg ggcggccggc ccggactcca ggtgatcgag
     6181 gacgattacg gcatcctgac catcagcacc ggcgcgccgg tcttcagctg ggaagagtgg
     6241 accggcaccg gcgccagcca tggcgacaac agcgtgctgc cggccggcga actggcggcc
     6301 gatctctacg aagacggccc gccggccctg gcccggagcg agcctggcga aatccggctc
     6361 gcctggaccc gggacgttcc gcccggcagc caggacggct cgcaggtgat ggtcgccctg
     6421 taccgcaatg aagacaaccg cttcttgacg gccgaaagca ttccgggcag cctcggttac
     6481 aacaaggatc tcaacctcgt ttacgacaac cggggccttc cgctggcgct ctgggcgcag
     6541 gttgattcca gcggcctggg cccggccacc agcgccgagg acttctacgc gcggctcaac
     6601 accggccaga tcaagtattc cgtctaccag ggcggcgtct ggtcgtcgcc ccagagcgga
     6661 ccgctggccc tggccggcaa caacagccga cccaccctgg gccggaccca cgacaaccgg
     6721 gtcatcgccg cctggatcaa ccgggcgggc ggcggcccgg cccggctgat ggccgccttc
     6781 tgggacggca acgcctggag cagcccggtc cagctggccg agggcaacct ggccggcacg
     6841 ccggccgccg gcacgctcgg cgaccggacc gccctcttct ggacccagga cgccgacccc
     6901 tccgagaccg ccagctggtt ccggatc
//
LOCUS       DAOQYN010000096         6910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_11339_length_6910_cov_5.255288, whole genome shotgun sequence.
ACCESSION   DAOQYN010000096 DAOQYN010000000
VERSION     DAOQYN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11339_length_6910_cov_5.255288"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PKN80_04970"
     CDS             <1..1223
                     /locus_tag="PKN80_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35401.1"
                     /translation="SDNLDQVYIYLQNTGQTSLIPRKVWLAGRDVTGQTRFLSRAIPP
                     GEKSLLRVDLPTPLARGKYVSIRLETADGSIGQALVRAFSVFPITSYSNPDTRLELYF
                     DPQPFHVKYQPAVSAAEIKALPPQTAWYLFSCPNCTDSEQNKPWGTSAVEIIKRAREF
                     YRLDPSRPNHSHVCEAYKERAYFIYGEVCDIMFINPYEVVFHDRDPARNGYFAGLGKL
                     ACEPRPLMTIQEAFARGPGAPFISPEQVRLGVYYQVAEGVKGISYYSKNQYTKYPALE
                     QEIGRINRELQAIKAYLKIGEPFAGLAVASRPEVLAKSIVAGDRGLVVILINTGYRTA
                     IRDEKRETTFTPQENFTVEVEVPGWLEIKQAVEIGPETRPVQLQKKNGKIIISVDRIN
                     LTKIFLLTDKGDPR"
     gene            1220..3460
                     /locus_tag="PKN80_04975"
     CDS             1220..3460
                     /locus_tag="PKN80_04975"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNQ35402.1"
                     /translation="MRRLFFLSLFLFLILVPQLEAALNVAIESPLDDSCFRVDTAVLV
                     NVRYTCDQAGYSLIFKVDGIQKSSVNASQGVDIQQVFVWDANAAGVFTLTVELRKTGC
                     YSASASIEATVFSVGLSGKTLLAKGKSTSLTATPTPGGLNGTYSWSVTQGSDKINLEG
                     SGNTISVNGLNVSGLINDVVVKVEFTASGASAAASATHSLTVFDVEISEGGPNPPDPY
                     IPVGGKRTYLAIVAPPSLDQAGPYTWSKSPSETEVISLYPVEYSCEVRADTYQTGSAT
                     LKARFDPGEEAYAEDSLGIFVFRPNIVVGEIGEGVETDTGAYLAKGGTKSCLLQLEGT
                     LPPDETVTLTCDKTGKVSLWDGETPKSFPYQCAVSALPKNLTIKGEATSDTIRDITLT
                     LTTARGGSDIAKITVFEIKLDWYDLSGEAKYDTGLYVPLNVDDDNNDTSLDKNQPNVF
                     QENDLKKLIIRKPSPSSLSGFIALTISSGTGRIKLWENSETDTKKITEVSSENYDIAG
                     DLQADTWLWVEGYDTSSIREVEIKLSYTAPDETIEEDKVKATVKIGTVLLDPGHGGTD
                     SGAVGPTGLLEKEPNLNLASKLKTRLQNAGVEVNMTRSTDETVSLSERNRQTKEEYKP
                     SFFFSIHNNAWENPVVRGTETFTTSVSYYELEDIFANQIQSKIHGVVLENDRGVKRYN
                     YQVLRHSENGKTDGNLSETTFITNAISEDRLREDDFKEAVAEAMKKAILKVLVENISY
                     PKQPNP"
     gene            3479..4432
                     /locus_tag="PKN80_04980"
     CDS             3479..4432
                     /locus_tag="PKN80_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35403.1"
                     /translation="MKSMLAITFGVLFSLTLFPVSLEAQTDGIKSIAAKEIWIIKNVG
                     VAPFFSPDGQWVAWTNAVERETRVYVAKVGTEKIIELGKTEELLGWLPKNSEYLMTAE
                     GFLSIKTKQISFLTAPVIESYFIKKAPSSNPGGNPYPTRIIYPFWMIRQGRIKKTYAT
                     EEPYCFSLLADKTSRKIVVFSKGIPLYEKEKAEMIFEFVPSPSADKFLLYYSPGENEQ
                     KVLEIKENKIHQLPDGTHFSWSPEGQKLIFAKLKDDGHFETSGELFLCNWDGTNITKI
                     VFEKERIRRAPSFGPSDLITYNFYDENYTLCIGVAQIIISP"
     gene            4878..5801
                     /locus_tag="PKN80_04985"
     CDS             4878..5801
                     /locus_tag="PKN80_04985"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ35404.1"
                     /translation="MEKLIIIGGGPAGLTAGLYAGRAGLAPLILERLAPGGQLGWAGA
                     IENYPGFPGGIGGLELAGRLRDHALQCGARIEASEVLGLAAGEGHFRLSGPSEYEAAA
                     VIVAAGSVPRPLGVPGETELTGRGVSYCATCDGPFFRGRRVAVVGGGNLMLQEALFLS
                     GLAAEVKLVVRAAAPAGSADLRRKLEESGRVEVVGESAPVGILGKDRVTGLQLRNLKS
                     GEESQLEVDGVFIFAGVRPGTGFLPPEVKRDAQGYLETDERMGTALPGLFACGDCRRR
                     PLRQVVSACAEGALAAVSAQHYLEELSGRAG"
     gene            5814..6443
                     /locus_tag="PKN80_04990"
     CDS             5814..6443
                     /locus_tag="PKN80_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNQ35405.1"
                     /translation="MHPLLEGLLDLVYPPACVGCRRRTGSSIFCPDCAAKIERLPGTP
                     GGRDFAALLYREPVVSAIHRLKYQGATFVLPVLGGFLNEAFTRLELFRVVDCLVPVPL
                     HPVRFRDRGFNQAELLAREILRHYDFPLLTRVLVRTRHTPSQTRLPEAARRRNVAGAF
                     RVRSASELAGRTCLLVDDVTTTGATLAAARDTLLAAGAAEVLTLALARS"
     gene            6591..>6910
                     /locus_tag="PKN80_04995"
     CDS             6591..>6910
                     /locus_tag="PKN80_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012765455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HNQ35406.1"
                     /translation="MVKIGINGFGRIGRLVLKAALSKKLDGVEFVAINDLTDSKTLAH
                     LFKYDSTFGVYPGSVKAGDGLLNIDGREIKVTAEKDPAQLPWKALGADIVIESTGRFT
                     EAEKA"
BASE COUNT         1580 a         1908 c         1953 g         1469 t
ORIGIN      
        1 tttccgataa cctcgaccag gtctacatct acctgcagaa caccggccag accagcctca
       61 tcccccggaa ggtctggctg gccggccggg atgtcaccgg ccagacgcgg tttctttcgc
      121 gggcgatccc gccgggtgaa aaaagcctgc tgcgggtgga tttgcctaca ccgctggccc
      181 ggggcaaata cgtttcaatc cgtctcgaaa ccgccgacgg atcaatcggc caggcgctgg
      241 tacgggcctt ctcggttttt cccattacct cctactccaa cccggacacc cgcctggagc
      301 tctatttcga cccgcagccg ttccacgtca aataccagcc ggcggtgagc gcggcggaga
      361 taaaggccct gccacctcaa accgcctggt accttttcag ctgccccaac tgcaccgaca
      421 gcgagcagaa caagccctgg ggcacctcgg cggtggagat catcaagcgg gcgcgggagt
      481 tttaccggct cgatccgagc cggccgaacc actcccacgt ctgtgaggcg tacaaggagc
      541 gggcctactt catctacggc gaggtctgcg acatcatgtt catcaacccc tacgaggtgg
      601 tctttcacga ccgggatccg gcccgcaacg gctactttgc cgggctgggc aagctggcct
      661 gtgagccgcg gccgttgatg accattcaag aggcctttgc gcgcggcccg ggcgcgccct
      721 tcatctcccc ggagcaggtt cggctggggg tgtactacca ggtggccgag ggcgtgaagg
      781 gcatctccta ctacagcaag aaccagtaca ccaaataccc ggccctggag caggagatcg
      841 gtcggatcaa ccgggaactc caggcaatca aagcctacct gaagatcggc gagccctttg
      901 cgggcctggc ggttgcctcc cggccggagg tgctggcgaa aagcatcgtt gccggcgacc
      961 gggggttggt ggtgattctg atcaacaccg ggtaccggac ggccatccgg gatgagaagc
     1021 gggagacgac cttcacaccc caggagaact tcacggtgga ggtggaggtg cccgggtggc
     1081 tggagatcaa gcaggcggtg gagatcggcc cggagacgag gccggttcaa ttgcagaaaa
     1141 agaatgggaa gatcatcatc tcggttgata gaatcaacct gaccaaaatc tttctgctga
     1201 ccgataaggg ggacccgaga tgagacgatt atttttcctg tctttgtttc tattcttaat
     1261 cctggtcccg caactcgagg cggccttgaa cgtcgccatc gagagcccgc ttgacgatag
     1321 ttgtttccgg gtggacacgg cggttttggt caacgtaaga tacacctgtg atcaggccgg
     1381 ctactccctg attttcaagg ttgatggcat acaaaaatct tccgtcaacg ccagccaggg
     1441 cgtggatatc cagcaggttt tcgtttggga cgcaaatgct gcgggtgttt tcacccttac
     1501 ggtggaactg cgcaagaccg gctgttacag cgcctccgcc agcatcgaag cgaccgtgtt
     1561 ttccgtcggc ctttccggaa agaccctgct ggcgaaaggg aagtcaactt ccctcaccgc
     1621 cacgcccacg cccggcggat tgaatgggac ttacagctgg tcggttaccc agggtagtga
     1681 taaaataaac cttgagggca gcggaaacac gatcagcgtg aacgggctta acgtgagcgg
     1741 cctgataaac gacgtggtgg tcaaggtgga attcaccgcc tcgggggcga gcgcggccgc
     1801 ttcggcgacg cattcgctga cggttttcga cgtggagatt tccgagggag gtccgaatcc
     1861 gcctgatccg tatatcccgg tcgggggcaa gagaacctac ctggccattg tggccccgcc
     1921 ctccctggac caggccggcc cttatacctg gagcaaatcg ccgtcggaga cggaggtgat
     1981 cagtctttat ccggtggaat acagttgcga ggtccgagcc gacacctacc agaccggttc
     2041 cgccaccttg aaggcccggt tcgatcccgg ggaggaggca tacgcggaag acagcctggg
     2101 aatctttgtc tttcgaccca acatagtggt gggtgaaata ggcgagggtg tggagacgga
     2161 caccggagct tatctggcca aaggcgggac caaatcctgc cttctgcaac tggaggggac
     2221 tcttccgccg gatgagaccg ttaccttgac ctgcgacaaa accggcaagg tctccctctg
     2281 ggacggcgaa acgccgaaat cttttcctta ccaatgcgcg gtttccgccc tgcccaaaaa
     2341 cctgacgatt aagggcgagg cgacttcgga caccatcaga gatatcaccc tgacgctgac
     2401 gacggccagg ggcgggagcg atattgcgaa aataacggta tttgaaataa aattggattg
     2461 gtatgattta tcaggcgagg caaaatatga tacgggttta tatgttccgt tgaatgtaga
     2521 tgatgataat aatgatacct ctctcgataa aaaccaaccc aatgttttcc aagagaacga
     2581 ccttaagaaa ctcattattc gtaaaccatc tccttcctct ttgtcaggtt tcattgcttt
     2641 aacaatttct tccgggaccg gccgaatcaa gctatgggaa aattctgaaa cggataccaa
     2701 aaaaatcacg gaagtttctt cggaaaatta cgatattgct ggtgatcttc aagctgatac
     2761 ttggttatgg gtagagggat acgataccag ttctatcaga gaggttgaga taaaactgtc
     2821 atacacagca ccagatgaaa cgattgaaga agataaggta aaagcaacgg tgaaaatcgg
     2881 gactgtttta ctggatcccg gacatggagg aacggatagc ggagctgttg ggcctacggg
     2941 ccttctcgaa aaagagccca atctgaacct tgcctctaag ctgaaaactc gattacagaa
     3001 tgccggtgta gaagtaaata tgacgagaag tacagatgaa acggtgagcc tttctgaaag
     3061 aaaccgtcaa acaaaagagg aatataaacc ttcttttttc ttttcaatac acaacaacgc
     3121 ctgggaaaac cccgtggtta gaggcaccga aacgttcact accagcgtat cctattatga
     3181 gctagaagat attttcgcaa atcagattca aagcaaaatt catggcgtag tattagaaaa
     3241 tgatagaggt gttaaaagat acaattatca agttttaagg cattctgaaa acgggaaaac
     3301 ggatggcaat ttgagtgaga cgacttttat tacaaatgcc atcagtgaag ataggttgag
     3361 agaggatgat tttaaagaag ccgtggcgga agctatgaaa aaagcgatat taaaggtttt
     3421 ggttgaaaat attagttatc cgaaacaacc caacccttag gaggtacctg tgaacaaaat
     3481 gaaatccatg ctggcaataa cgtttggggt actgttttct ttaacccttt tccccgtttc
     3541 tttagaagct caaacagatg ggataaaatc catcgcggcg aaggaaatat ggataataaa
     3601 aaatgtgggg gtcgccccct tcttttcccc ggacgggcaa tgggtcgcct ggactaatgc
     3661 cgttgagcga gagacaaggg tgtatgtggc aaaagtcgga acagaaaaaa ttatcgaatt
     3721 gggcaaaacc gaagaattgt tggggtggct tccgaagaac agcgaatacc ttatgaccgc
     3781 cgaggggttc ctgtcaataa aaacgaaaca aatttcattt ttaaccgccc cggtaatcga
     3841 gagctatttt ataaagaaag caccttcatc caatccaggg gggaatcctt atcctacccg
     3901 cataatttat cctttttgga tgattcggca ggggagaata aaaaaaacct atgcaacaga
     3961 agaaccctat tgcttttcat tgttagccga taaaacaagc agaaaaattg ttgtgttttc
     4021 caaggggata cctctttacg aaaaggaaaa agctgaaatg atttttgaat ttgttccttc
     4081 gccttcagcg gataaatttc ttctgtatta ttcccccgga gaaaacgagc agaaagtact
     4141 cgagataaaa gaaaataaaa tccaccagct tcccgatggc acccatttct cgtggtcacc
     4201 ggaaggtcag aaattaatat tcgcaaaatt aaaagatgat ggacattttg agacctctgg
     4261 cgaactgttt ttatgcaact gggatggaac taatataacg aaaattgttt ttgaaaaaga
     4321 gagaatccgg cgagcaccat catttggtcc atcggatttg ataacctata atttctacga
     4381 cgagaattac accctttgca ttggtgtagc ccaaattata atttcccctt aataagccct
     4441 tggtgatttt tggactgcac ccctaaaact tgacacttat gtttagaaca cctggcagag
     4501 cctaaccgtt cccccactgt ggacagaaag gaaccgttgt catgaactcc gtcaaatatt
     4561 tcggggagtc taggcaggga tcgcatcttt acatctgaca tccgggataa acgatttttg
     4621 attaattccc tccgactgac gcgccaccac ccggtttatg aaggcctcca accctgtcaa
     4681 ttgtcattag ttgattcctc caacacccgg aatttattag gatattcaca aattcccata
     4741 ttcatatttg attcccgatt ttcgattttc cgaacatctc cgtcccggtt tgacaggccg
     4801 ggagtttctg gtattaacta tgtttcggaa tcaggcggcg tcaattactt tttaacgagg
     4861 ttttggggag aagaagcgtg gaaaaactca tcatcatcgg cggcggcccg gccggcctga
     4921 ccgccggcct ctatgccggc cgcgccggcc tggcgccgct gatcctggaa cggctggccc
     4981 cgggcggcca actgggctgg gccggcgcga tcgagaatta ccccggcttc ccgggcggca
     5041 tcggcggcct ggagctggcc ggccgcctgc gcgaccacgc cctccagtgc ggggcccgga
     5101 tcgaggcgtc cgaggtgctc ggactggcgg ccggggaggg gcatttccgg ctctccggtc
     5161 cgtccgaata cgaggcggcc gcggtgatcg tggccgccgg ttcggtgccc cggccgctgg
     5221 gcgtgccggg cgagaccgaa ctgaccggcc gcggcgtctc ctactgcgcc acctgcgacg
     5281 gtcctttctt ccgcggccgg cgggtggcgg tggtcggtgg cggcaacctg atgctccagg
     5341 aggcgctctt cctctccggc ctggcggccg aagttaagct ggtggtgcgc gccgcggcgc
     5401 cggccggctc ggccgacctg cgccggaagc tggaggagag cggccgggtg gaggtagtcg
     5461 gcgaatcggc gccggtcggg atcctcggca aggaccgggt gaccgggctg cagttgcgca
     5521 acctcaagag cggcgaagag agccagctgg aggttgacgg ggtcttcatc ttcgccggcg
     5581 tgcgccccgg taccggtttc ctgccgccgg aggtcaagcg cgacgctcag ggttacctcg
     5641 agaccgacga gcggatgggt acggcgctgc ccggcctctt cgcctgcggc gactgccgcc
     5701 gccggccgct gcgccaggtg gtgagcgcct gcgccgaggg ggccctggcg gcggtctccg
     5761 cccagcacta cctcgaagaa ttatccggcc gggccggctg accggtaccg gcgatgcacc
     5821 cgctcctgga aggcctcctg gacctggtct atccgcccgc ctgcgtcggt tgccgccgcc
     5881 ggaccgggtc ttccatcttc tgcccggact gcgcggcgaa gatcgaacgc ctgcccggga
     5941 ctcccggcgg ccgggacttc gccgcgctcc tttaccggga gccggtcgtt tcggccatcc
     6001 accgcttgaa ataccagggt gcgacctttg tcctgcccgt cctgggcggc ttcctgaacg
     6061 aggccttcac ccggctggag ctcttccggg tggtggactg cctggtcccg gttccgctgc
     6121 acccggtccg cttccgggac cggggtttca accaggcgga actgctggcc cgggaaattc
     6181 tccgccatta cgattttccc ctgcttaccc gcgtcctggt ccggacgcgc cataccccat
     6241 cccagacccg tctccctgaa gcggcgcgcc gccgcaacgt ggccggggcc tttcgggtcc
     6301 ggtccgcttc cgaactggcc ggccggacct gcctgttggt tgacgatgtc accaccaccg
     6361 gcgccaccct ggccgccgcc cgggatactc tgctggccgc cggcgccgcc gaggtcctga
     6421 ccctggccct ggcccgaagt tgacaccggg cggccgtttt tgataaaatt taccccgttg
     6481 ggcccccgtt tttttgaact gttttattaa taaaaaatcc aaaaaaagag gcagcgaaaa
     6541 accttgtttt tatttcaacg ctatttccca caacccacgg gaggcatgaa atggtgaaga
     6601 tcggaatcaa cggatttgga cggatcggcc ggctggtgct caaggccgct ctgagcaaga
     6661 agctggacgg cgtggaattc gtggccatca acgacctgac cgacagcaag accctggccc
     6721 acctcttcaa gtacgactcg accttcggcg tctatcccgg ttcggtgaag gccggcgacg
     6781 gcctgttgaa catcgacggc cgcgagatca aggttaccgc cgagaaggac ccggcccagc
     6841 tgccctggaa ggccctgggc gcggacatcg tcatcgagtc caccggccgc ttcaccgagg
     6901 ccgagaaggc
//
LOCUS       DAOQYN010000097         6891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_11395_length_6891_cov_3.839087, whole genome shotgun sequence.
ACCESSION   DAOQYN010000097 DAOQYN010000000
VERSION     DAOQYN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11395_length_6891_cov_3.839087"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1338)
                     /gene="ribD"
                     /locus_tag="PKN80_05000"
     CDS             complement(220..1338)
                     /gene="ribD"
                     /locus_tag="PKN80_05000"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNQ35407.1"
                     /translation="MAEPNERDRYLKQALRLARRGSGLVSPNPMVGAVLVRGGRVVGA
                     GWHRFYGGAHAEVEALRQAGARAAGATLYVNLEPCDHTGKTPPCTEAIIAAGVKRVTA
                     AMADPNPLVSGRGFRRLRGAGLEVEAAGGAAAEAAAALNRAYLVRIAEGRPFLALKIA
                     QSLDGRIADAAGRSKWITGPEARAYTRRLRFEYDAILVGINTVLEDDPGLDYQPPARP
                     PAAEKTYWKIILDSRARLPLTARCLKRGRVAVAVGRTADPDRVKRLEAAGALVLAGAG
                     ERPDLPEVMKKLYKLNIGSVLVEGGAEVFGACLDAGLTDRVYLCLALILLGGRTARPA
                     VLGSGRRLANPARFRLTGTRRLAEDRLLVLDRADGPGT"
     gene            complement(1331..2185)
                     /gene="panC"
                     /locus_tag="PKN80_05005"
     CDS             complement(1331..2185)
                     /gene="panC"
                     /locus_tag="PKN80_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNQ35408.1"
                     /translation="MKIASDVAGLRAALKRYRAEGKSVGLVPTMGAFHAGHLALMRTS
                     AQEMDRTVTSLFVNPIQFGPAEDLARYPRCPESDARQAAEAGVDLLFTPGAAEMYPPG
                     FRSRVEVAGLSDRLCGIYRPGHFSGVATVVLKLLNLVQPDRAYFGWKDAQQLLIIKRL
                     VRDLNLPVEIVGMATVREKDGLALSSRNRYLEPAERRAAPRLYQVLREIADRLAGHPE
                     TAIEPVLAGARRRLEKSALIRLQYLEAVDPETLDPAGDESREVLVALAAWIGSTRLID
                     NIMVSRHG"
     gene            complement(2169..2633)
                     /gene="folK"
                     /locus_tag="PKN80_05010"
     CDS             complement(2169..2633)
                     /gene="folK"
                     /locus_tag="PKN80_05010"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNQ35409.1"
                     /translation="MARIILGLGSNLGRREGNLKRAVRELTAAGIRVLKVSRPTASAP
                     AEGVGGGEFLNAALLAETDLEPEALLDLLQRTEARLGRPASHRPGEARTIDLDIIYYR
                     DRRLETERLKLPHPRRLQRRFVLEPAAEVAPDFVDPELNLTLEEIARHENRQ"
     gene            complement(2647..3855)
                     /locus_tag="PKN80_05015"
     CDS             complement(2647..3855)
                     /locus_tag="PKN80_05015"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF003242.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HNQ35410.1"
                     /translation="MKYLLIIGDGMADYPLPELENRTPLEVAPTPNLDRLASESGRLG
                     MVRTIPRGTMAASDTAFLSIMGLDPRQNHPGRGPLEAINLGLRLTDEEVAFRLNLVTQ
                     DEKKMVDSSAGHISDGEAGRLMAVLQEHFQGRRMRFVPGKSYRNLLIMERPPFDWRKL
                     VTTPPHDILGRPLTPYLPKGPGGEFLIGMMEEAARIFAEHPINRTRIDLGENPANLVW
                     PWGQGGPAQLPAFQPRYGLTGFTVSAVDIVKGIGLAVGLEAVSVPGATGYLDTNYAGK
                     VEAAIEGLKRHDFGVLHVEAPDEASHSGQWRLKVKAITDFDQRVVGPAVSALRTEFPE
                     SRVMVMADHITSLRTRTHTPDPIPFLISGTGVVGRGAATFSEKTAGAGRRYLREGWRL
                     MEFFLRPEIT"
     gene            complement(3888..4787)
                     /gene="nadA"
                     /locus_tag="PKN80_05020"
     CDS             complement(3888..4787)
                     /gene="nadA"
                     /locus_tag="PKN80_05020"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNQ35411.1"
                     /translation="MNDIVSEIQAWKEKRRAVILAHNYQPPEIQALADFLGDSLDLSR
                     LAAGTEHETVVFCGVKFMAETAKILAPEKTVLLPDLQAGCPMADMVRPEDIRERRAAY
                     PGAPVVTYINSSAEVKAESDYICTSANAVRLVSALAEETVLFFPDQNLGDFVQERTAK
                     KMILWPGYCPTHQVFIPEELAALKAANPGAVLIAHPECRREVRRMADEVLSTGGMVKW
                     ARESRAKQAIVATEIGMLTRLRRENPAIEYLPGSSRAVCPNMKKISLEKVLWSLKELA
                     PAIEVPEATARRSRLALERMLKF"
     gene            complement(4795..>5073)
                     /locus_tag="PKN80_05025"
     CDS             complement(4795..>5073)
                     /locus_tag="PKN80_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ35412.1"
                     /translation="RQGDPVAARVWQETGFYVGVMLANLVNIFNPEAIIIGGGVAQAG
                     EPLFAAIEASIKKHSFPILTTRLKLLPASLGAESGLKAAAALVFYPPE"
     assembly_gap    5074..5173
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5174..5604)
                     /locus_tag="PKN80_05030"
     CDS             complement(<5174..5604)
                     /locus_tag="PKN80_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ35413.1"
                     /translation="MKTAIGLDLGGSFIKYGRLDQAGRIISQGQVDSGGEKGRDEVLK
                     RMAGAVAGLLEGTAPGEVAGIGIGTPGLVDNRGSVFLAPNLPEWDNLPLAEIFKKKFR
                     LPVVVENDVNSFTWGEFKFGAGKPFRDIIAITLGTGLGGGVV"
     gene            complement(5601..6815)
                     /locus_tag="PKN80_05035"
     CDS             complement(5601..6815)
                     /locus_tag="PKN80_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNQ35414.1"
                     /translation="MAGVPIGKDIYWVGAVDWNLRDFHGYRTGRGSSYNAHLILDEKK
                     CLVDTVKSPFTGRLLENISALVKPGEIDYIISNHLELDHSGAILEILRHCPKARLLAT
                     GHWIEGAEKYFGPGLPVQPVKTGDTLSLGRRTLQFIEAPMLHWPDSLFTYLPEDGILL
                     PNDAFGQHYASGSRFDDEVPEAVLMDEAARYYANIILPYSNLVTRMLGRIGEMKLDIR
                     LIGPSHGIIWRRPEKILAAYRDWAAGRTRPKAVVAYDTMWGSTAMLAESIAEGLQSAG
                     LETVVMPLKASNRSDVAAEILEAAAFALGTPVLNNLPLPSVADLYTYLKGLRPRKKQW
                     LAFGSYGWNREIIPRFAQEIKEAGFEVELPAVAARFRPDGGELAAARAAGVRLAGLAA
                     GAVETEPKEKPE"
BASE COUNT          957 a         2376 c         2291 g         1167 t
ORIGIN      
        1 ccttctcgtt gcctaccgtg atgatcatgc gcgcctcctt ttttgggttg accgcggccc
       61 ccgccccgct ccgctccggg gtccggccgg gccgcgttcc ttcaggattt cccgccccgc
      121 cggcccgggg cgcctttcaa taacaattat aattattata atgataacta aaagtcaaag
      181 accgccgccc gtcttgatgc gcttgttttt taaaaaggtt caggttcccg ggccgtcggc
      241 ccggtccagc accagcagcc ggtcttcggc caggcgcctg gttccggtca gccggaagcg
      301 cgccgggttg gccagccggc gtccggagcc gagcaccgcc ggccgggcgg tccggccgcc
      361 cagcaggatc agggccaggc agaggtaaac ccggtcggtc aggccggcgt ccaggcaggc
      421 accgaagacc tcggccccgc cctcgaccag caccgagccg atatttaatt tatacaactt
      481 tttcatcact tccggcaagt ccggccgttc cccggcgccg gccagcacca gcgccccggc
      541 cgcctccagc cgcttcaccc ggtccgggtc ggccgtccgc cccaccgcca ccgccacccg
      601 tccccgcttc aggcaccggg ccgtcagcgg cagccgggcc cggctgtcca ggattatctt
      661 ccagtaggtc ttctccgccg ccggggggcg ggccggcggc tggtagtcga ggccgggatc
      721 gtcctcgagc acggtattga ttccgaccag gatggcgtcg tactcgaagc gcagccggcg
      781 cgtgtaggcg cgggcctccg ggccggtgat ccacttggat cggccggccg cgtcggcgat
      841 ccggccgtcc aggctctggg cgatcttgag ggccaggaag gggcgccctt cggcgatccg
      901 gaccaggtag gcgcggttca gggccgcggc cgcctcggcc gccgcgccgc cggccgcctc
      961 cacctccagc ccggccccgc gcagccgccg gaagccccgg cccgatacca gcgggttggg
     1021 atcggccatg gccgccgtca cccgcttcac gccggcggcg atgatggctt cggtgcaggg
     1081 cggggtcttg ccggtgtggt cgcagggctc caggttgacg tagagcgtgg cgccggccgc
     1141 ccgggcgccg gcctgccgga gcgcctccac ctcggcgtgc gccccgccgt agaaacggtg
     1201 ccagccggcg cccaccaccc ggccgccacg caccaggacc gcgcccacca tcgggttggg
     1261 gctgaccagt ccggagcccc gccgggccag ccgcagggcc tgtttcaggt agcggtcgcg
     1321 ctcgttcggt tcagccatgg cgggagacca tgatgttgtc gatcagccgg gtggaaccga
     1381 tccaggcggc cagcgccacc aggacctccc ggctttcatc cccggctggg tccagcgtct
     1441 ccgggtcgac cgcctccagg tactggagcc ggatcagggc ggatttttcc agccgccgcc
     1501 gcgccccggc taggaccggc tcaatcgccg tttccgggtg gccggccagc cggtcggcga
     1561 tttcccgcag gacctggtag agccgcgggg ccgcccggcg ttcggccggc tccaggtaac
     1621 ggttgcgcga actcagggcc agcccgtcct tttcccgcac cgtcgccatt ccgacgatct
     1681 ccaccggcag gttgaggtcc cggaccaggc gcttgatgat cagcagctgc tgggcgtcct
     1741 tccagccgaa gtaggcccgg tcgggctgga cgaggttcag cagcttgagg accacggtgg
     1801 ccaccccgct gaaatggccg ggacggtaaa tgccgcagag gcggtccgag agtccggcca
     1861 cctcgacccg gctgcggaag cccggcgggt acatttcggc cgcgccgggc gtgaagagca
     1921 ggtcaactcc ggcttcggcc gcctgacgcg cgtccgactc cgggcagcgc gggtaccggg
     1981 ccaggtcttc ggccgggccg aactggatcg ggttgacgaa gaggctcgtc accgtgcggt
     2041 ccatttcctg cgccgaggtc cgcatcaggg ccaggtgccc ggcgtggaag gcacccatgg
     2101 tcggcaccag cccgacgctc ttcccttcgg cccggtaacg cttgagcgcc gccctcaggc
     2161 cggcgacgtc actggcgatt ttcatggcgc gcgatctcct ccagggtcag gttcagttcc
     2221 gggtcgacga aatccggcgc gacctcggcg gccggttcca ggacgaaccg ccgttgaagc
     2281 cggcgcggat ggggcagctt cagcctttcg gtctccagcc ggcggtcccg gtagtagatg
     2341 atgtccaggt cgatcgtccg ggcctcgccc ggccggtggg aagcgggccg gcccagccgg
     2401 gcctcggtcc gttgcagcag gtccagcagg gcctccggtt ccaggtccgt ttcggccagc
     2461 agggcggcat tcaggaactc gccgccgccg acgccctcgg ccggcgcgct ggccgtcggc
     2521 cgcgatactt tcagcactct gatcccggcc gccgtcagtt cccggacggc ccttttcagg
     2581 ttgccttcac ggcggcccag gttggaaccc aggccgagga ttatcctggc catccgcctg
     2641 gtatgttcag gtgatttcgg ggcgcaggaa gaactccatc agccgccacc cctcgcgcag
     2701 gtaacggcgg ccggccccgg ccgtcttctc gctgaaggtg gccgcgccgc ggccgaccac
     2761 cccggttccg ctgatcaaaa atgggatcgg gtccggggtg tgggtccggg tgcgcagcga
     2821 ggtgatgtgg tcggccatga ccatgacccg cgactcgggg aattcggtcc ggagcgcgct
     2881 gaccgccggc cccaccaccc gctggtcgaa atcggtgatt gccttgacct tgaggcgcca
     2941 ctggccgctg tggcttgcct cgtccggcgc ctcgacgtgg aggacgccga aatcgtgacg
     3001 tttcaatccc tcgatggccg cttccacctt gccggcgtag ttggtgtcca ggtagccggt
     3061 cgcccccggc acgctgaccg cctccaggcc gaccgccagg ccgatgccct tgacgatgtc
     3121 caccgccgag acggtgaatc cggtcagccc atagcgcggc tggaaggccg gcagctgcgc
     3181 cgggccgccc tgcccccagg gccagaccag gttggccggg ttttcgccca ggtcgatccg
     3241 ggtccggttg atcgggtgtt cggcgaagat ccgggccgcc tcctccatca tcccgatcag
     3301 aaactcgccg cccggccctt tgggcaggta aggagtcagc ggccgtccca ggatgtcgtg
     3361 gggcggggtg gtgaccagct tccgccagtc gaagggcggc cgttccatga tcagcaggtt
     3421 gcggtagctc ttgccgggca cgaagcgcat ccggcgtccc tggaagtgct cctggagaac
     3481 cgccatcagc cggcccgcct cgccgtcgct gatgtggccg gccgaggagt ccaccatctt
     3541 cttttcatcc tgggtcacca ggttgaggcg gaaggccacc tcctcgtcgg tcagccgcag
     3601 gccgaggttg atcgcctcca gcgggccgcg gcccgggtgg ttctggcggg gatccaggcc
     3661 catgattgag aggaaggcgg tgtcgctggc ggccatcgtt ccccgcggga tggtccggac
     3721 catccccagc cgcccggatt cggaggccag ccggtccagg ttcggggtgg gcgcgacctc
     3781 cagcggggtc cggttttcca gttccggcag cgggtagtcg gccatgccgt cgcctatgat
     3841 aagcaggtat ttcatctccg gaatcccgtc ctttcctctg gttgatttta aaatttcagc
     3901 attcgttcca gggccagccg gctgcgccgg gccgtcgcct ccgggacctc gatggccggc
     3961 gccagctcct tcaggctcca gagcaccttc tccaggctga tcttcttcat gttcgggcag
     4021 acggcccggc tcgagccggg caggtactcg atggccgggt tctccctgcg gagccgggtg
     4081 agcatgccga tctcggtcgc cacgatggcc tgtttcgccc ggctctcccg ggcccatttc
     4141 accatgccgc cggtcgagag gacctcgtcg gccatccggc gcacctcgcg ccggcattcc
     4201 gggtgggcga tcaggaccgc gcccgggttg gccgccttca gggccgcgag ttcctcggga
     4261 atgaagacct ggtgggtcgg gcagtagccc ggccagagga tcattttttt ggcggtccgt
     4321 tcctggacga agtcgcccag gttctggtcc gggaagaaga gcaccgtctc ctcggccagg
     4381 gccgaaacca ggcgcaccgc gttggccgag gtgcagatgt aatcgctctc ggccttgacc
     4441 tcggccgagg aattgatgta ggtgaccacc ggcgcgcccg ggtaagcggc gcgccgctcc
     4501 cggatgtcct ccggccgcac catgtcggcc atcgggcagc cggcctggag atccggcagc
     4561 aggacggtct tttccggggc cagtatcttg gccgtttcgg ccatgaactt caccccgcag
     4621 aagaccaccg tttcatgctc ggtgccggcg gccagtcgcg aaaggtcgag cgagtcgccg
     4681 aggaaatcgg ccagcgcctg gatctccggc ggctggtaat tgtgggccag gatgaccgcc
     4741 cgccgttttt ccttccaggc ctggatctcg gagacgatgt cgttcatctg gagcctattc
     4801 cggcgggtag aagaccagcg ccgcggccgc cttcaggccg ctttccgcgc ccagggaggc
     4861 cggcagcagc ttgaggcggg tggtcaggat cggaaaggag tgctttttta tcgaggcctc
     4921 gatggccgcg aagagcggtt cgcccgcctg ggcgaccccg ccgccgatga tgatcgcctc
     4981 ggggttgaag atgttgacca ggttggccag catcaccccg acgtagaagc cggtttcctg
     5041 ccagacccgc gccgcgaccg ggtcgccctg ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnaccaccc cgccgcccag cccggttccc agcgtgatgg cgatgatgtc
     5221 ccggaagggc ttcccggccc cgaacttgaa ctcgccccag gtgaaggagt tgacgtcatt
     5281 ctccaccacc accggcaggc ggaacttctt cttgaagatc tcggccagcg gcaggttgtc
     5341 ccattccggc aggttgggcg ccaggaagac cgagccccgg ttgtcgacca ggccgggcgt
     5401 gccgatgccg atcccggcca cttcgcccgg ggccgtgccc tccagcaggc cggcgaccgc
     5461 cccggccatg cgcttgagaa cttcgtcccg tcccttctcg ccgccggagt ccacctgccc
     5521 ctggctgatg atgcggccgg cctggtccag ccgcccgtac ttgatgaagg aaccgcccag
     5581 gtccagtccg atggccgtct tcattccggc ttctccttcg gttcggtctc gaccgcaccg
     5641 gccgccagtc cggccagccg gacccccgcc gcgcgggcgg ccgccagctc gccgccatcg
     5701 ggccggaacc gggccgccac cgccggcagc tccacttcga agccggcctc ctttatctcc
     5761 tgggcgaagc gcggaatgat ctcccggttc cagccatagg aaccgaaggc cagccactgc
     5821 ttcttgcggg ggcgcagccc cttgaggtaa gtgtagagat cggccaccga gggcagcggc
     5881 aggttgttga gcaccggggt gcccagggcg aaggccgcgg cctccaggat ctcggcggcc
     5941 acgtcgctgc ggttactggc cttcagcggc atgaccacgg tctccaggcc ggccgactgc
     6001 agcccctcgg cgatcgactc ggccagcatg gccgtgctgc cccacatggt atcgtaggcc
     6061 accaccgcct tcggccgggt ccggcccgcg gcccagtccc ggtaagccgc caggatcttc
     6121 tcgggccgcc gccagatgat gccgtggctc gggccgatga ggcggatgtc cagcttcatt
     6181 tcgccgatcc ggccgagcat cctggtcacc aggttggagt aaggcaggat gatgttggcg
     6241 tagtaccggg ccgcctcgtc catcaggacc gcttcgggca cctcgtcatc gaagcgggag
     6301 ccggaggcgt aatgctggcc gaaggcgtcg ttgggaagca ggatgccgtc ctccggcagg
     6361 taggtgaaga ggctgtccgg ccagtggagc atcggcgcct cgatgaactg gagggtgcgc
     6421 cggcccagcg aaagcgtgtc gccggtcttg accggctgga ccggcaggcc cggcccgaag
     6481 tacttttcgg ccccttcgat ccagtggccg gtggccagga ggcgggcctt cgggcagtgg
     6541 cgcaggatct ccaggatggc gcctgaatgg tccagttcca ggtggttgga gatgatgtaa
     6601 tcgatctcgc cgggcttgac cagggccgag atattctcca ggagccggcc ggtgaacggg
     6661 ctcttgacgg tgtccaccag gcacttcttc tcgtccagga tcaggtgggc gttgtaactg
     6721 ctgccccggc cggtgcggta gccgtggaag tcgcgcaggt tccagtccac tgccccgacc
     6781 cagtagatat ccttgccgat cggtacgccg gccatcaggc tcccccttct tccgttttat
     6841 ctttaagctt tttttccccg acgggcgcgc cgggaagttc gcgcagcgag g
//
LOCUS       DAOQYN010000098         6874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_11429_length_6874_cov_4.067459, whole genome shotgun sequence.
ACCESSION   DAOQYN010000098 DAOQYN010000000
VERSION     DAOQYN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11429_length_6874_cov_4.067459"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /gene="amrB"
                     /locus_tag="PKN80_05040"
     CDS             <1..238
                     /gene="amrB"
                     /locus_tag="PKN80_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNQ35415.1"
                     /translation="REKDRYALEAVLSLDPAGLLDRVVEREISMCGAGPAAVMLETAR
                     RLGATEARLAAYATSGEVSGDYDSVVGYAGVTVV"
     gene            333..1082
                     /gene="larB"
                     /locus_tag="PKN80_05045"
     CDS             333..1082
                     /gene="larB"
                     /locus_tag="PKN80_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNQ35416.1"
                     /translation="MNEKDLSELLRDYRSGRKSLQRTLDELKALPYRDLGFARLDTHR
                     ELRFGFPEVIYAPGKTDAQLAGIVKAIAAGRRPDRFLITRLAAERAPDLKLIHPALQY
                     FPEARIATGPLPRPSGRGYVMVVSAGTADLPVAAEAELTARVLGSRVKRLHDVGVAGL
                     HRLLDSVPLIQRATCLVVVAGMEGALPSVVGGLCGRPVIGVPTSVGYGASLSGLTPLF
                     TMLNSCSPNVSVVNIDNGFGAGVLAHLISRR"
     gene            1109..1768
                     /locus_tag="PKN80_05050"
     CDS             1109..1768
                     /locus_tag="PKN80_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35417.1"
                     /translation="MEKALQQLETEIKKELDRKDRAREAVLPACRELVRMASEVIKEV
                     HRHKWPEARSHLVKAGLSLREIQERVAKHPDLRFAGFVTMAEKEYAEAAITLAVLNEK
                     RLPTLDEIGVHAPEYLNGMAESIGEMRRHLLDQLRKDRLAEAERILQVMDSFYHFLFS
                     LDYPDAILRGLRRMVDSDRGILERTRGDLTTTLVQEKLRRELRQAAGRNGGKRPARAA
                     R"
     gene            1818..2450
                     /gene="hisH"
                     /locus_tag="PKN80_05055"
     CDS             1818..2450
                     /gene="hisH"
                     /locus_tag="PKN80_05055"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNQ35418.1"
                     /translation="MNGYIWVVDYGLGNLRSVSKALERVGARVEVSPDPNRLDGAGAV
                     VLPGVGAFDAAVANLRHGGLLAPLAGYLASNRPFLGICLGFQLLFSGSAEGREPGFGI
                     IPGRVQRFPNRVKVPQIGWNRVAFQTDSPLFRGLGEEAYFYFVHSYYGVTERAIGLSE
                     YGLSFTAALQSGAIFATQFHPEKSGETGLAVLANFVAWVREYPGRKPPES"
     gene            2454..3800
                     /locus_tag="PKN80_05060"
     CDS             2454..3800
                     /locus_tag="PKN80_05060"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ35419.1"
                     /translation="MKTIEALREGLDRLPVLDTHEHLVPERQRLEGGFDVLSLLLEIY
                     IGGDLLSSGMSRNDLNLVVGRGGRPVPLEEKWKRLAPYWPQVSQTGYGRMIRLALKRF
                     FGLDRLDRPGLMKAQAIFESYNRAGIYRKILEEAGIRKVITQRTEPLEPGESELFELV
                     IRPVGLGSLDRPGIENLEKRLGRSLPTLDAFLEGQRAFLKSARATGCLGLKTAAWCVE
                     EAELETAFSDLVENGSVGRPRLGPARAAYQKLAAGNGLRPAEQWTLNSSLMHHLADCA
                     RELDLVMVIHTGAVWNAWIDFRVYNPLHIVPLLRLHPETKFDVYHAGIPWCREMAMIA
                     KVFPNAWVNLTWAHIISPAMTISFLDELLDMVPPGKILGFGGDYVHSVENVIGHLEIA
                     RENLAAVLARRIEKGLMDRSEAVAAARGWLWDNPARLYGIADPQPKTTARRTKEKT"
     gene            3802..5193
                     /locus_tag="PKN80_05065"
     CDS             3802..5193
                     /locus_tag="PKN80_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35420.1"
                     /translation="MDPKPLKGNLHCHADESGPHKSGFPVEQVLKLFREEAYDLLGLT
                     EHRLEAARTTYRKARQLKDGPPILIRGYELSHRRPRFHVASLYFPGRRNLKVCAHPVR
                     THGDKWLEEFTKLEGKLRIGAVELDNYLAFQPEWLESYRPVQARYPLVCDSDFHNDHF
                     MMRNACTLYFAPERSPLGVWQAIQERGLLCIWPDLATWRLRFLAQNETARRWLDSAPE
                     AAGLKMRPAVRPEAMSAGDIGGYFHQELHDLNNPPGAATLTPGFGGRLMSFTWHGQHL
                     LSPLNSTWLDGPNGYAGSPTFESQFQAWQIESSAPDQATLSYRVRDGHWRGLVMKRQV
                     KLDRTGLTVTGWQENPTGRAIETDLTLGLNLHKKFGVQSRAVIKTAAGEQEFNTPMRA
                     IVKEPGPIFVPGLAEGIGLRIAIGGRRSHELHLWDNRHEMRVFVSFYFEPHTIPAGGR
                     SEPSVLRLEPVTG"
     gene            5264..6793
                     /gene="guaA"
                     /locus_tag="PKN80_05070"
     CDS             5264..6793
                     /gene="guaA"
                     /locus_tag="PKN80_05070"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNQ35421.1"
                     /translation="MVIILDFGSQYTQLIARRVREARVYCDILPYNTPLADLRKRKPE
                     AVIISGSPASVSEVGHPTAADGIFNLEIPILGLCYGMHLMTDTLGGEVKSAGKREYGR
                     TMFQVLKRDDLFHGLPARFIAWMSHGDYSARIPAGFEVIGATRSCPAAAFRHRTRPLY
                     GLQFHPEVSHTPKGAEIFHNFLFRIAGCKPNWTMASFIREETTRIRERVGKDQVVSAL
                     SGGVDSSVLSVFLHRALGNRLHCIFVDTGLLRAGERESVETTFRKHFHINLTVIDARE
                     RFLKKLAGVADPERKRKIIGEEFIRIFEEEARRLGGIKYLAQGTLYPDLIESQSVFGG
                     PTSRIKSHHNVGGLPEKMDLKLIEPFKYLFKDEVRELGAELGLPAEITGRHPFPGPGL
                     AVRILGEITRERLEILRQADRIYIAEIKEAGWYDRIWQALAVLLPIKTVGVMGDARTY
                     ENVIALRAVCSKDGMTADFTPIPHPLLGKIANRIINEVKGVNRVVYDVSSKPPATIEW
                     E"
BASE COUNT         1283 a         2308 c         2161 g         1122 t
ORIGIN      
        1 ccgggaaaag gaccggtacg ccctcgaagc ggtcctgtcg cttgacccgg ccggactgct
       61 ggaccgggtc gtggaaaggg agatctcgat gtgcggcgcc gggccggcgg cggtaatgct
      121 ggaaacggcg cggcgcctcg gcgccaccga ggccaggctg gccgcctacg ccaccagcgg
      181 cgaggtctcg ggagactacg attcggtggt cggctacgcc ggcgtgaccg tcgtttagcc
      241 gtcctttcca cccggccctt cttttgacac cccccgcgtg aaaaaagtaa actatctgca
      301 agtcagacgc tgtttcccac gcacccgtga ccatgaacga aaaggacctt tccgaattgc
      361 tgcgcgacta ccgttccggc cgaaagagcc tgcagcggac cctggacgaa cttaaagccc
      421 tcccataccg ggacctgggc tttgcgcggc tcgacacgca ccgggaactc cgtttcggat
      481 tcccggaagt catctacgcc ccgggcaaga ccgacgccca gctggccggt atcgtcaagg
      541 cgatcgccgc cggccggcgg cccgaccgtt tcctgatcac ccgactggcg gccgaacggg
      601 cgccggatct gaaactgatt catcccgccc tccagtactt tccggaagcc cggatcgcca
      661 ccggaccgtt gcccaggccc tccggccggg gctatgtaat ggtggtcagc gccggcaccg
      721 ccgacctccc ggtggccgcc gaggcggaac tgaccgcccg ggtcctgggc agtcgggtca
      781 agcgcctcca cgacgtcgga gtggccggcc tgcaccggct gctggactcg gtgccgctca
      841 tccagcgggc cacctgcctg gtggtggtgg ccggcatgga gggggccctg ccttcggtgg
      901 tcggcggcct ctgcggacgg ccggtgatcg gggtcccgac ctcggtcggc tacggggcct
      961 ccctgagcgg attgacgccg cttttcacca tgcttaactc ctgcagtccc aacgtttcgg
     1021 tggtcaatat cgacaatggt ttcggcgccg gggttctggc gcacctgatc agccggcgct
     1081 gaagattcgt ccccccggaa aggcaaacat ggaaaaggcg ctccagcagc tggaaacgga
     1141 gataaagaag gaactggacc gcaaggaccg ggcccgggag gccgtgctgc cggcctgccg
     1201 ggagttagtg cgcatggcct cggaggtaat caaggaggtc catcggcaca agtggccgga
     1261 ggcccggagt cacctggtaa aggccgggct cagcctccgg gaaatccagg agcgggttgc
     1321 gaaacacccg gatctccgtt tcgcgggatt cgtgaccatg gccgaaaagg agtacgctga
     1381 ggcggccatc accctggcgg tcctgaatga aaaacggctg cccaccctcg atgaaatcgg
     1441 cgtgcacgcg cccgaatacc tgaacgggat ggccgaatca atcggggaga tgcggcgcca
     1501 cctgctggac cagctgcgca aggaccggct ggctgaggcc gaacggatac tccaggtgat
     1561 ggatagtttt taccactttc tcttctcgct cgactacccg gacgccatcc tgcgcggact
     1621 tcgccgcatg gttgacagcg accggggcat cctcgagcgg acccggggcg acctgaccac
     1681 gaccctggtc caggaaaagc tgcgccggga gctccggcag gcggccggac gaaacggcgg
     1741 aaagaggccg gcccgggctg cacgctaacc cgggccgccg gccgcccgca ttccctgtct
     1801 tttcacgctc cgtcaaaatg aacggctaca tctgggtggt ggattacggg ctgggcaacc
     1861 tgcgcagcgt cagcaaggcg ctcgaaaggg tgggcgcccg ggtggaagtc agcccggacc
     1921 cgaaccggct ggacggggca ggcgcggtcg ttctgccggg cgtcggggcc ttcgacgcgg
     1981 cggtcgccaa cctgcggcac ggcggcctgc tggccccgct ggccggatac ctcgcctcca
     2041 accggccctt cctgggcatc tgcctgggct tccagctgct cttctccggc agcgccgagg
     2101 ggcgcgaacc gggcttcggt atcatcccgg gccgggtcca gcgtttcccg aaccgggtca
     2161 aggtgcccca gatcggctgg aaccgggtcg ccttccagac tgactcgccg ctcttccggg
     2221 gcttgggcga agaagcctac ttttatttcg tgcactccta ttacggggtg accgaacggg
     2281 cgatcggcct ctccgagtac gggctttcct tcacggcggc cctgcagtcc ggagcgatct
     2341 tcgccaccca gttccacccc gaaaaaagcg gtgaaaccgg cctggcggtc ctggctaatt
     2401 tcgtggcctg ggtgcgcgaa taccccggcc ggaaaccacc ggagtcctga aaaatgaaga
     2461 ccatcgaagc gctgagggaa ggcttggacc ggctgccggt cctggatacc cacgagcacc
     2521 tcgttccgga acggcagcgc ctcgaaggcg gcttcgacgt tctttcgctg ctcctggaaa
     2581 tatacatcgg cggcgacctg ctctcgtccg gaatgagccg gaacgacctg aacctggtag
     2641 tcggccgcgg cggccggccg gttccgctcg aagaaaaatg gaaacgcctg gcaccctact
     2701 ggccccaggt cagccagacc ggctacggcc ggatgatccg cctggccctg aaacgctttt
     2761 tcggccttga ccggctcgac cggccgggcc tgatgaaggc ccaggccatc ttcgagtcgt
     2821 acaaccgggc cggcatctac cggaaaatcc tggaagaggc cggcatccgg aaggtaatca
     2881 cccagcgcac cgaacccctg gagccgggcg aatccgaact cttcgagctg gtgatcaggc
     2941 cggtcggact gggcagcctg gaccggcccg ggattgaaaa cctggaaaag cggctgggcc
     3001 gttccctgcc tacgctcgac gcctttctgg aaggccagcg cgccttcctg aagtcggccc
     3061 gggcaaccgg atgcctgggt ttgaagacgg ccgcctggtg cgtggaagag gcggaattgg
     3121 aaacggcctt cagcgacctg gttgaaaacg ggtcggtcgg ccggccccgg ctgggtccgg
     3181 cccgggccgc ctaccagaaa ctggcggccg gaaacggcct gcgcccggcc gagcaatgga
     3241 cgctcaactc cagcctgatg caccacctgg ccgactgcgc ccgggaactg gacctggtga
     3301 tggtcatcca caccggggcg gtctggaatg cctggatcga tttccgggtt tacaacccgc
     3361 tgcacatcgt gccgctgctg cgcctgcacc cggagacgaa gtttgacgtc taccacgccg
     3421 gcatcccctg gtgccgggag atggccatga tcgccaaggt cttcccgaac gcctgggtca
     3481 acctgacctg ggcgcatatc atcagcccgg ccatgaccat ctccttcctc gacgagctgc
     3541 tggatatggt tccgcccggc aagatcctgg gcttcggtgg cgattatgtc cacagcgtcg
     3601 aaaacgtgat cggccacctc gagattgccc gggaaaacct ggccgccgtc ctggcccggc
     3661 ggattgaaaa gggcttgatg gaccgatccg aggccgtggc ggccgcccgg ggctggcttt
     3721 gggataaccc ggcccgcctt tacggaattg ccgatcctca acccaaaacc accgcccggc
     3781 gaaccaagga gaagacctga gatggatccc aagccgctca aaggaaacct gcactgccac
     3841 gccgacgaat ccggacccca caaatcagga ttccccgtcg aacaggtctt gaaattattc
     3901 cgtgaagaag catacgacct gctcggcctg accgagcatc gcctggaggc ggcccgcacc
     3961 acctaccgga aggcgcgcca gctgaaagac gggcccccga ttctcatccg gggttacgag
     4021 ttgagtcacc gccgcccccg tttccacgtg gccagcctct acttcccggg ccggcgcaac
     4081 ctcaaggtct gcgcccaccc ggtccgcacc cacggcgaca agtggctgga agaattcacg
     4141 aaactggaag gaaaactccg gatcggcgcc gtcgaactgg acaattacct cgccttccag
     4201 ccggagtggc tcgaaagcta ccgcccggtc caggcgcgct acccgctggt ctgcgactcc
     4261 gattttcaca acgaccattt catgatgagg aatgcctgca ccctctattt cgccccggaa
     4321 agaagtccgc tgggcgtctg gcaggcgatc caggagcgcg ggttgctgtg catctggccg
     4381 gacctggcca cctggcggct gcgcttcctg gcccagaacg aaaccgcccg gcgctggctg
     4441 gattccgccc cggaggcggc cgggctcaag atgcggccgg ccgtccggcc cgaggcgatg
     4501 tcggccgggg atatcggcgg ctactttcac caggaactcc acgacctgaa caacccgccc
     4561 ggcgcggcca ccctgacccc cggcttcggc ggccggctga tgagcttcac ctggcacggc
     4621 cagcacctgc tttcgccgtt gaattcaacc tggctggacg gaccgaacgg ttatgccggc
     4681 agcccgactt tcgaaagcca attccaggcc tggcagatcg aatcgtccgc cccggaccag
     4741 gcgaccctga gttaccgggt gcgggacggc cactggcgcg gcctggtcat gaaacggcag
     4801 gtcaagctcg accggaccgg tctgaccgta accggctggc aggagaaccc gaccggccgg
     4861 gcaatcgaaa ccgacctcac gctcgggtta aacctgcaca aaaagttcgg tgtccagagc
     4921 cgggccgtga taaaaaccgc cgccggagag caggaattca acaccccgat gcgggccatc
     4981 gtgaaggaac ccggcccgat cttcgtcccc ggcctcgcgg agggcatcgg ccttcgaatc
     5041 gccattggcg gccgacgctc acacgaactg catctctggg acaaccggca cgaaatgcgg
     5101 gtcttcgtct ccttctactt cgagccgcac accattccgg ccggcggccg atccgaaccg
     5161 tccgtactcc ggctggaacc ggtaaccggc tgacctcatg ataaaataat caaaacccgc
     5221 ccttcggcgg aacccggcgc caacctttcc cgcggagccc aacatggtca taattcttga
     5281 tttcggatcc cagtacaccc agttgatcgc ccgtcgggtt cgggaagccc gggtttactg
     5341 cgacattctg ccttacaaca ctccgctggc cgacctccgg aaacggaaac cggaggcggt
     5401 catcatctcc ggcagcccgg ccagtgtctc ggaggtcggg cacccgaccg ccgccgatgg
     5461 tatttttaac ctggaaatac ccatactggg actctgttac ggcatgcacc tgatgaccga
     5521 caccctgggg ggcgaagtca aatcggccgg caagcgggag tacggccgga ccatgttcca
     5581 ggtgttgaag agagatgacc tcttccatgg cctgccggcc cgtttcatcg cctggatgag
     5641 ccacggcgac tactcggccc ggatcccggc cggtttcgag gtcatcggcg ccacccgcag
     5701 ttgcccggcc gccgccttcc gccaccgcac caggccgctc tacggactcc agttccaccc
     5761 cgaagtcagc catacgccga aaggcgcgga gatctttcac aacttcctct tccggatcgc
     5821 cggctgcaag ccgaactgga caatggcctc gttcatccgc gaggagacga cccggatccg
     5881 ggaacgggtg ggcaaagacc aggtggtcag cgccctctcc ggcggggttg attcctcggt
     5941 cctgtccgtc ttcctgcacc gggcgctggg aaaccggctc cactgcatct tcgtggacac
     6001 cggcctgctg cgcgccggcg aacgggagag cgtggagacc accttccgca agcactttca
     6061 catcaacctg accgtgatcg acgcccggga aaggttcctc aaaaaactgg ccggggtggc
     6121 cgacccggaa cggaaacgca agattatcgg ggaggagttc atccggatct tcgaagagga
     6181 ggcccggcgc ctgggcggaa tcaagtacct ggcccagggg accctttacc cggacctgat
     6241 cgaaagccaa tcggtcttcg gcggccccac ctcgcgcatc aagagccacc acaacgtggg
     6301 cggtctgccg gagaagatgg acctgaagtt gatcgaaccc ttcaagtatc tcttcaagga
     6361 cgaggtgcgg gaactggggg ccgagctcgg cctgccggcc gaaatcaccg gacgccatcc
     6421 ctttccgggg cccggcctgg cggtaagaat cctgggcgag attacccggg agcggctcga
     6481 aattctgcgc caggccgacc gcatctacat cgcggaaatc aaggaggccg gctggtacga
     6541 ccgcatctgg caggccctgg cggtgctgct gccgatcaag accgtgggcg tgatgggcga
     6601 cgcccgaacc tatgaaaacg tgatcgccct gcgggccgtc tgcagcaagg acggcatgac
     6661 cgccgacttc accccgattc cgcaccccct gctgggcaag atcgccaacc gcatcatcaa
     6721 cgaggtgaag ggcgttaacc gggtggtcta cgacgtcagc tccaagccgc cggcgacgat
     6781 tgaatgggag taagcggcac ggcggccttc gcttcgcgtc cactctgcgt ccggggcaaa
     6841 agctacgcga ctcgggaacg agggctctcc gtgt
//
LOCUS       DAOQYN010000099         6818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_11537_length_6818_cov_5.670856, whole genome shotgun sequence.
ACCESSION   DAOQYN010000099 DAOQYN010000000
VERSION     DAOQYN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11537_length_6818_cov_5.670856"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..1102
                     /locus_tag="PKN80_05075"
     CDS             338..1102
                     /locus_tag="PKN80_05075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019505458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ35422.1"
                     /translation="MKQWYELLFENYGRKYDQEPFTQGTLGECDFIEQEISRDKSLKI
                     IDIGCGTGRHSIELAKRGYRVTGVDLSEAQLRRAREKTKKLDLNIDFQKQDARNLPFE
                     GEFDLAIMLCEGGFPLMETDEMNFEILKNATKALKEKGKLMFTTLNGLFPLFHSVKEF
                     YESAANKEGNAAYENCSFDLMTFRDHSTVVFEDDAGTKKELTCNERYYVPSEIAWLLK
                     TLGYRKIEILGAKLGAYSRQDKLTTEDFEMLVVAEK"
     gene            1320..2030
                     /locus_tag="PKN80_05080"
     CDS             1320..2030
                     /locus_tag="PKN80_05080"
                     /inference="COORDINATES: protein motif:HMM:NF020785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1961 family protein"
                     /protein_id="HNQ35423.1"
                     /translation="MPLEYADREELFSDGLTDLANWHHEGVGTMELLPGGGMRLHCLG
                     SRQGEEACLAFFRPDLPDRVAVSYDIIIHSHGGLVINYIALRGLKGEDVIRDSDRLES
                     RTGVMKNYYAAKWGLQSYHLSFSRFNDRGMHTNTSNWRRNPGARLVGHGDDPVRELGR
                     RYRIRLTKDAGFLQFFVDGAFAHAAVDRDTGRRPIPDYGKFAFRLVGSDIKADIFGFR
                     VHRIDRHPEVDKNAEDYV"
     gene            2023..3228
                     /locus_tag="PKN80_05085"
     CDS             2023..3228
                     /locus_tag="PKN80_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HNQ35424.1"
                     /translation="MSEASFGREHYHLVERAREKLPASRWHEMLVYLCGLAERSIMPS
                     SGPFRRPWLTVGPGYIAHPAFGHWDLVHACLDLVRFSPELVRDQLRNLFETAGPDGML
                     PGLSYGPGKRRPDFWANHKLSHPPLWPQAAEEYFRVARDLEFLKECFEFGRRNLAWWE
                     AHRRVAGGGFYYRDILDRTWESGIDDGLRFKTPAERPFACVDATSHLYGYYEMMEKWA
                     GRLELDAAAYREKRVGLAEFIQSEFWDERAGFFHDVGRPGRPKTFEGFLPMAFGAASE
                     AQSIRLHEHLMNESEFYAFHPIPTVALNEPDWSFDMWRGPAWNSRTFWILRGSHRYGH
                     RKEVGEIGLRTLDRTLEHFRASGTIHEFYNSRSIDQSVLVRKGNPAGPCREYLGHNPL
                     NAFFYLVTE"
     gene            3246..3722
                     /locus_tag="PKN80_05090"
     CDS             3246..3722
                     /locus_tag="PKN80_05090"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNQ35425.1"
                     /translation="MIPETRFMRRAIEEARKNLVEDPAGGPFGACLVRSGRVVAVARN
                     TVLAGDATCHAEVNAIRMASRELGSYDLAGLVIYSTTEPCPMCFSAIHWARISAIFYG
                     TGIRSAAARGFNELRLSNRQLKRLGGSPVAVAGGILRSECLELFEAWDRLAGRPSY"
     gene            3770..4984
                     /locus_tag="PKN80_05095"
     CDS             3770..4984
                     /locus_tag="PKN80_05095"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNQ35426.1"
                     /translation="MKTKERSEFLKYQRLYRSALLDDALPFWMRHSPDRERGGYYHCL
                     DRDGSVICTDKYMWLENRQIWTFAWFYNRLEKRPEWLGLAKLGADFLKAHGRDGNGDW
                     YFALTETGQPLVQPYNIFSDCFAVIAFAEYARASGDQEALELAIRTYRRIQERQANPK
                     GRYNKQVPGVRPLENLAFPMINLNISRILNEVRPNPVYSRVIEQSLDRIMNRFLDPEC
                     RVVHENLTPEGRRRDDIYEGRHLIPGHGLEAMWFVMEAAAGAKDWSTVRRAAEGVKWC
                     LEKGWDREYGGIYYFLDRLDRPHFELQWDMKLWWPHLEALVATLLGYRLTGDPELLDW
                     FRKVHEYAWRVFPDPEYGEWFGYANRRGEVNNRCKGGRFKGCFHLPRALYLVTELCRE
                     LADTSAGTGQNG"
     gene            complement(4926..5633)
                     /gene="truB"
                     /locus_tag="PKN80_05100"
     CDS             complement(4926..5633)
                     /gene="truB"
                     /locus_tag="PKN80_05100"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNQ35427.1"
                     /translation="MKNTDLDLILPVRKPAGSSTYDLIRAFKRAAGFQGKIGHGGTLD
                     PFASGVVLLLLERATGRFEEIRTWEKVYQAGLRLGAVSSTGDPQGTISAVETAGSFRG
                     GRKKILPILEKFTGRFEQKIPAYSAAQSGGERLYRLARAGQPVEKSKPVEIRRLEMVA
                     WRWPLLTLRVTCLGGVYIRQLAEDIGNEMGCGAYLYFLERERVGQYTLKECLEPEEFS
                     RFDRFRPKYPPAPGTTR"
     gene            5726..>6818
                     /locus_tag="PKN80_05105"
     CDS             5726..>6818
                     /locus_tag="PKN80_05105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ35428.1"
                     /translation="MLMKGGWWSGVSRNVRVLGVVSLFNDIASEMIYPVVPIFLTAVL
                     GAPVAVVGLIEGFVEATASLLKLASGWFSDRFRRRKPFVVGGYAFSTFSKLLLGVAYV
                     WPMVLAARFIDRLGKGIRTSARDALIVESTANGSRGLAFGFHRAMDSTGAVIGPLLAI
                     LLLKIFRDNLRTVFLISVLPAAVGVLLLIFLLRESGVRLEGRPGRFRLSWRELSPEFK
                     VFLLVSVLFGLGNSSDVFLILRAKDLGLTTVLAIFAYVLYNLGYVLFSIPAGAVADRI
                     GPRKVLTAGFLVYALVYLGFGLTGDARAVWILFPVYGVYIAFTDGVGKAYISNLVAGE
                     KIGTAFGIYQMATGLCTFLASFIAGLLWTR"
BASE COUNT         1239 a         1958 c         2200 g         1421 t
ORIGIN      
        1 gcgtgcttcg ctctgcttca tggcgattat tgtttctgaa tcttattcag acaaatcggc
       61 gggttccgcc tgtgcttgaa aggcaggttt cggcggataa aggccgattt tacctgccat
      121 agctttagcg aatggccgac ccgagccgca gtgggaggcg agggcgccaa atccctccct
      181 ctccgccatt tttcgcaggg caaaaaatgg cgagatctcg cctgtccgcc ataaaatctg
      241 gcgagacgag gcggatccgc aaaaccccac tcttacgcag ggatttggtg ggagcgaagt
      301 cagatgacgc ggttttaccg gataaaaggg ggagaccgtg aaacaatggt acgagctgtt
      361 atttgagaat tacgggcgta agtatgatca agagcctttt acccagggga cgctcggcga
      421 atgcgatttt atcgagcagg aaataagccg ggataaatca ctgaagatca tcgacatcgg
      481 ttgcggaacc ggccgccatt cgatcgaatt ggcaaaaaga ggataccgcg taaccggggt
      541 tgatttgtcc gaggcacaac ttagaagggc aagggaaaaa acaaaaaaac ttgatctgaa
      601 cattgatttt cagaagcagg acgccagaaa ccttcccttt gaaggcgaat ttgatctggc
      661 gatcatgctc tgtgagggcg gttttccttt aatggaaacc gacgagatga attttgaaat
      721 attaaagaat gcgaccaagg cgttaaaaga gaagggcaag ttaatgttca cgaccttgaa
      781 cgggttgttc ccgctttttc attccgtgaa ggaattttat gagtccgcgg ccaacaaaga
      841 aggcaatgcg gcgtatgaaa attgttcttt cgatctgatg acgttccggg accacagcac
      901 cgtcgtcttc gaggacgatg cgggaactaa aaaggaatta acctgtaacg agcgttatta
      961 cgtgcccagc gagatcgcct ggcttttgaa gacgctcggc taccggaaga tagaaatttt
     1021 aggagcgaag ttgggcgcct actcccggca ggataaactg accaccgagg atttcgagat
     1081 gctggtggtc gcggagaagt aaacccgcct tccggtttcc ttttttcaga ccgttttccc
     1141 caccttcaga cgcgttgagg cctccggcaa cccttcggtc cggacggtcc ggcgccgctc
     1201 ccccttttcc cgtcccgccg gcgccgcttg ccaggcaagc ggcggaaagt gtataaagat
     1261 gtaggggggc ttcgacgccg ggtttggcgt cggggggccc gcgaaggagg agatgaccaa
     1321 tgccgctgga atacgccgac cgggaagagc tcttcagcga cggcctgact gacctggcca
     1381 actggcacca cgagggggtc ggcaccatgg agctgctgcc ggggggcggt atgcgccttc
     1441 attgcctggg cagccggcag ggggaggagg cctgcctggc cttctttcgg cccgacctgc
     1501 ccgaccgggt ggcggtttcg tacgatatca tcatccacag ccacgggggc ctggtcatca
     1561 attacatcgc cctgcgtggt ttgaagggcg aagatgtcat ccgggactcc gatcggctgg
     1621 aatcgaggac cggcgtgatg aagaattatt acgccgccaa gtgggggctg cagagctacc
     1681 acctctcatt cagccggttc aacgaccggg ggatgcacac caacacctcg aactggcgcc
     1741 gcaatccggg cgcccggctg gttggccacg gggacgaccc ggtgcgtgaa ctgggacggc
     1801 gttaccggat ccggcttacc aaggacgccg ggttcctgca gtttttcgtg gacggtgctt
     1861 ttgcccacgc cgcggtggat cgtgataccg gccggcggcc gatacccgat tacggaaaat
     1921 tcgcttttcg gctggtcggt tccgacatca aggccgacat tttcggattt cgggtgcacc
     1981 ggattgatcg gcatccggag gtggacaaga acgcggagga ttatgtctga ggcatcgttc
     2041 ggccgggaac attaccacct ggtggagcgg gcccgggaaa agttgccggc ctcgcgctgg
     2101 catgagatgc tggtctacct gtgcgggctg gccgaacgtt cgataatgcc gtcgtccggg
     2161 cctttccggc ggccctggct gacggtggga ccgggataca tcgcccaccc ggccttcggc
     2221 cactgggacc tggttcatgc ctgcctggac ctggtccgct tcagcccgga attggtgcgc
     2281 gaccagctcc ggaatctctt cgagacggcc ggaccggacg ggatgctgcc gggactttct
     2341 tacggtccgg gcaaacgccg gccggatttc tgggccaatc acaaattgag ccatccgccg
     2401 ctctggcccc aggcggccga ggagtatttc cgggtggccc gcgacctgga gtttttgaag
     2461 gagtgtttcg aattcggccg acggaacctg gcctggtggg aggcgcaccg ccgggtggcc
     2521 ggcggcggtt tttattaccg ggacatcctc gaccgcacct gggagagcgg aattgacgac
     2581 gggttgcgct ttaaaactcc ggcggaaagg ccgtttgcct gcgtggacgc cacctctcac
     2641 ctgtacggct attacgaaat gatggagaag tgggccggcc gactggagct ggacgccgcc
     2701 gcctaccggg agaagcgggt tggcctggcc gagttcatcc agtccgaatt ctgggatgag
     2761 cgggccggtt tttttcatga cgtcggccgg ccgggtcggc ccaagacctt tgagggattc
     2821 ctgccgatgg ccttcggggc cgcttccgag gcgcagtcga tccggttgca cgagcacctg
     2881 atgaacgaat ccgaatttta tgccttccac ccgattccga ccgtggcact caacgaaccg
     2941 gactggagtt tcgacatgtg gcgcggtccg gcctggaatt cgaggacctt ctggatcctc
     3001 cggggcagcc atcgttacgg ccaccggaag gaggtcggag agatcggtct tcggacgctg
     3061 gaccgcaccc tggaacactt ccgggccagc gggaccatcc acgaatttta taattcccga
     3121 tcgattgacc agtcagtcct ggtccgcaag gggaacccgg ccggtccctg ccgggagtac
     3181 ctgggccaca accctttgaa tgcgttcttt tatctggtga ccgaataggc atgcggcggc
     3241 agggattgat tccggaaacg cgcttcatgc gccgggcgat cgaagaggct cggaagaacc
     3301 tggttgaaga cccggccggc ggccccttcg gcgcctgcct ggtcaggtcc ggccgggtgg
     3361 tggccgtggc ccgcaacacg gtcctggccg gcgatgccac ctgccatgcc gaggtgaatg
     3421 ccatccggat ggcctcgcgg gaattgggct cctatgacct ggccggattg gtgatttatt
     3481 cgaccaccga accctgcccg atgtgcttca gcgccatcca ctgggcgcgg atttcggcta
     3541 ttttctacgg caccgggatt cggtcggcgg ccgcccgcgg cttcaatgag ctgcgccttt
     3601 ccaaccgcca gctgaagcgg ctgggcggca gtccggtggc ggtggccggc gggatcctgc
     3661 gttccgaatg cctggaactg ttcgaggcct gggatcgcct ggcgggacgg cccagttact
     3721 gaccgtccgc cggcggccgg gtttcgatgt ccgggcctgg gaggaaaaga tgaaaacgaa
     3781 ggaacggtcg gagttcctga agtatcaacg cctttatcgt tccgcgctcc tggatgatgc
     3841 gctgcccttc tggatgagac attcgccgga ccgggagcgg ggtgggtatt accactgcct
     3901 ggatcgcgac ggttcggtga tctgcaccga caagtacatg tggctggaga accggcagat
     3961 ctggaccttc gcctggtttt acaaccggct ggaaaagagg ccggaatggc tcgggctggc
     4021 caaactgggc gccgattttc tcaaggcgca cggccgggac ggaaatggag actggtactt
     4081 cgccctgacc gaaaccggcc agcccctggt tcagccctac aacatcttct ccgactgctt
     4141 cgcggtgatc gccttcgccg agtatgcccg ggcctccggt gaccaggagg ccctggaact
     4201 ggcgatccgc acctaccgtc gcatccagga gcgtcaggcc aacccaaagg gtcgttacaa
     4261 caagcaggtg ccgggcgtcc ggcccctgga aaacctggcc tttccgatga tcaacctcaa
     4321 catcagccgg attttgaacg aggtccggcc gaatccggtt tacagccggg tgatcgaaca
     4381 gagccttgac cggatcatga accggtttct cgacccggag tgccgggtgg tgcacgagaa
     4441 cctgaccccg gaaggccgcc gccgggatga catctacgaa gggcgccatc tgattcccgg
     4501 ccacggtctg gaggcgatgt ggttcgtgat ggaggcggcg gccggcgcga aggattggtc
     4561 cacggtgcgc cgggcggccg agggagtgaa gtggtgcctc gagaagggct gggaccggga
     4621 atacggtgga atctattatt tcctggatcg gctggaccgg cctcatttcg aactgcagtg
     4681 ggacatgaag ctctggtggc cgcacctgga ggcgctggtg gcgacgctgc tcgggtaccg
     4741 cttgaccggg gatcccgaac tgcttgattg gttccggaag gttcatgagt acgcctggcg
     4801 ggttttcccg gatccggaat acggcgagtg gtttggttat gccaaccggc ggggcgaggt
     4861 taacaaccgg tgcaaggggg ggcgtttcaa gggctgcttt cacctgcccc gggccctcta
     4921 cctggtcacc gagttgtgcc gggagctggc ggatacttcg gccggaaccg gtcaaaacgg
     4981 ctgaactcct ccggttccag gcattctttc agggtgtatt ggcccacccg ctcccgttcc
     5041 agaaagtaca ggtaggcccc gcagcccatt tcatttccga tatcttcggc cagctgccgg
     5101 atgtataccc cgcccaggca ggttactcga agggtcagca ggggccagcg ccaggccacc
     5161 atttccagcc ggcgaatttc gaccggcttg cttttttcca ccggctggcc ggcccgggcc
     5221 agacggtaga ggcgctctcc gcccgattga gcggccgaat aggccggaat tttctgctca
     5281 aaacggccgg tgaatttttc caggatcggg agaatttttt tacggccgcc ccggaaactc
     5341 ccggccgttt caacggcgct gattgttccc tgggggtcgc cggtcgagga gaccgccccc
     5401 agccgcagac cggcctggta gaccttttcc caggtccgga tctcctcaaa gcggccggtg
     5461 gcccgttcca ggaggagaag gaccacgccg gaggcgaacg ggtccagcgt accgccgtgg
     5521 ccgatcttgc cctggaagcc ggcggcccgt ttgaaggccc ggatcaggtc gtaggtgctg
     5581 ctgcccgccg gtttgcgcac cggcaggatg aggtcgaggt ccgtgttttt catggtcgat
     5641 tagattatat ccccggcggg ccggtccggc aagcccgcgg ctgtgctata ataaacgaaa
     5701 aaaacacttt tttgaaaggg gtccgatgct gatgaaggga ggctggtggt cgggggtgtc
     5761 ccggaacgtc cgggtgctcg gggtggtgag tctcttcaat gatattgcct cggagatgat
     5821 ttacccggtg gtgccgatct tcctgaccgc ggtcctgggt gcgccggtgg cggtggtggg
     5881 cctgatcgag gggtttgtcg aggcgaccgc cagccttctc aaactggcct cgggctggtt
     5941 ttccgaccgc ttccgccgcc gcaaaccgtt cgtggtgggc gggtacgctt tttccacgtt
     6001 ctccaagctg ctgctggggg ttgcctatgt ctggccgatg gtgctggcgg ccaggttcat
     6061 cgaccggctt ggaaagggca ttcgcacctc ggcccgagac gcgctcatcg tggagagtac
     6121 cgccaacgga agccggggcc tggccttcgg ctttcaccgg gcaatggatt cgaccggtgc
     6181 ggtgatcggg ccgttgctgg ccatcctgct cctgaaaata ttccgggata acctgcggac
     6241 ggtcttcctg atctcggtcc tgccggcggc cgtgggcgtg ctgcttctta ttttcctgct
     6301 ccgtgaatcg ggggtgcggc tggagggccg gcccggccgg ttccggttaa gctggcggga
     6361 actctctccg gaattcaagg tttttttgct ggtgagcgtc ctcttcggcc tcggcaacag
     6421 ttccgatgtc ttcctgatcc tgcgggccaa ggatctgggt ttgaccaccg tcctggccat
     6481 ctttgcctac gttctctata acctgggcta tgtgcttttt tcgataccgg ccggcgcggt
     6541 ggccgaccgg atcggccccc gaaaggtgtt aacggccgga ttcctggtct acgcgctggt
     6601 ttatctggga ttcggattga ccggggatgc ccgggccgtc tggattctct tcccggttta
     6661 cggggtttac atcgccttca ccgacggagt cggcaaggcc tatatttcca acctggtcgc
     6721 cggcgaaaag atcggtaccg ccttcggcat ttaccagatg gcgaccggcc tctgcacttt
     6781 cctggcctcc ttcatcgccg gcctgctctg gacccgcc
//
LOCUS       DAOQYN010000100         6722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_11745_length_6722_cov_4.161692, whole genome shotgun sequence.
ACCESSION   DAOQYN010000100 DAOQYN010000000
VERSION     DAOQYN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11745_length_6722_cov_4.161692"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKN80_05110"
     CDS             <1..204
                     /locus_tag="PKN80_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNQ35429.1"
                     /translation="ERAGRFTVEEIIAGLTAKLKRRHPHVFGTERAGSARAALFSYRR
                     AKRLEKKTADSGAASRRGRRPGI"
     gene            complement(201..1895)
                     /locus_tag="PKN80_05115"
     CDS             complement(201..1895)
                     /locus_tag="PKN80_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ35430.1"
                     /translation="MIRRGWTWLILGLTLAFFVLFFFGPLFQALKGGFLHGGKFTPEF
                     FLEIFRNPIYREGVSNSFRLALATTLLVAALGLPMAFLAARCDFPAKRLLGGLVLVPM
                     VLPPFVGAIGISQMLGRFGALNALLVQLGFLDWSRPVDWLGQGRFWAVAATQALHLYP
                     IFYLNASAALANIDPALEEAAENLGASGGRRFWRITLPLAAPGLFAGGSLVFIWAFTE
                     LGTPLMFDYTRVTAVQIYDGIKDIGDNPLPYALVTFMMITSILIYAAGKFLFGRQSAA
                     MLGKSAGAAETRRLTGGPAWLASGLFLLVFLAAALPHLGVILTSFSRGWYQSALPSAW
                     TLGHYREALGHALTLPSIKNSLYYATLALTLDMVLGVAIAYLVVRGNFRGRGLLDLLA
                     MLPLAVPGIVLAFGYIAISQPGRLFAAFNPANNPTLLLIIAYAVRRLPYVVRSVTAGL
                     QQTAEALEESAQNLGASTWHTLRRITLPLISANLIAGGLFAFSFAMLEVSDSLMLAQK
                     QAFFPITKALYELFQILGTGRYVAAALGVWAMLFLTVTILAANSLLGRKLGALFRI"
     gene            complement(1892..3391)
                     /locus_tag="PKN80_05120"
     CDS             complement(1892..3391)
                     /locus_tag="PKN80_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ35431.1"
                     /translation="MMKRLYGWLAAFLLILALPFLLKPPAESNPAGARRLVAVSPHNE
                     ATRYEFENAFRGWYRQRYGETADIEWRNLGGTSEIVRFIDSEFEAAGRVGREGIGIDV
                     FFGGGSFDHARQARKGHLQPCAIRTRHPEWLNPEIMPQQFSGETLHDSEDLWYGCCLS
                     GFGICYNEDLVRSLGTAAPERWEDLARPEFFGRIALADPSKSGSINKAFETLIQEQMA
                     ASAARAGLDPGRAGAEELAEGWRTALNLLKRIGANCRYFTDSASKVPLDVAQGNAAAG
                     MCIDFYGRFQSETVEREEGSKRMRYFTPTGGSSFSADPVGILRGAPDAELARRFVEFL
                     LSPEGQRLWNYRIGTPGGPVKYSLRRLPIRKDAYGEAGRPYRSDPDVNPYLQAGSFVY
                     RPGWTGPMFGLIRLLVRAMCIDTHPELVAAGRAIQAAGGPSACPEAMARLEQLPANAE
                     YPAALRETTAALGEKKNEIAITREWTVFFRENYRQAETLARAAADRRRP"
     gene            complement(3396..4478)
                     /locus_tag="PKN80_05125"
     CDS             complement(3396..4478)
                     /locus_tag="PKN80_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ35432.1"
                     /translation="MKITIEKINKNFGRTAALRDVSFQVEDGEFFFILGPSGCGKTTL
                     LRAVAGFVKTDSGRILFDNRLVNDLPPYRRNTGMVFQNYALWPHLTVAQNVAFGLTVP
                     GRNLPPKERGERVTAALAMVHLENYAGRRPGQLSGGQQQRVALARALVIKPDCLLLDE
                     PLSNLDAKLRREMRTELKRIVRETGLTALYVTHDQKEALSMADRCAVLNLGRVEQIGS
                     PQELYRKPASLFTADFIGATNLVPGRVTETGGGNLTVETAAGTWTGPNPAGRFQAGDR
                     VTLSIRPEAINFNAPPAGSVNRKEARLVESVYLGESIEYLLEPAAGIRLRACRLNPEN
                     SETAAGSRLDFWVDPAEVVVLAGVAA"
     gene            complement(4563..4964)
                     /locus_tag="PKN80_05130"
     CDS             complement(4563..4964)
                     /locus_tag="PKN80_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35433.1"
                     /translation="MPTKKLNWYQKALALILAFGALYLLGALTQRESIIEQLQNAAGD
                     HLGVILLVKSQTERDRPFVKALREARPKLGGKAITLTTTGPAMPGITAETKLPLIRVL
                     DAHGTVMDELQEVADLARLPELARELTEHHH"
     gene            complement(5042..6223)
                     /locus_tag="PKN80_05135"
     CDS             complement(5042..6223)
                     /locus_tag="PKN80_05135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ35434.1"
                     /translation="MIKFGNGRGRWIYLFPALMDFSLAALSLFGVLKAAEMGASAFLL
                     GALGAVWGLVYFLVSLLLGRLINASNRYASMIAGCLLMVLVTVGLMWADRLALLFFFF
                     ALYGVAASLFYIGFQLFMAERVRLPIHQACGFYTLSWSLGIGAGSLAQGYLVTRGPVA
                     ALMPAVLSSLLVILVLEIDRHWPIRPGPTGPDPAEPPGPPVPASRRALFVRLGWLLFL
                     NASFIGIAIRILVPKLILDLPGSGPAAAGTLVCLVFSIQAVSGFLLSGRHTLNYGFRP
                     ILATSLAGGAGLLAAWLWPGWPGMLTLAAGFGLYCGYAYSAGVFYALSQTEQSGRNIG
                     INEAIVGVAGVLGPLAGGAALDRGPDVFHAAMLSFFLLLLAGQGLLFARSRRRPDETR
                     G"
     gene            6425..>6722
                     /locus_tag="PKN80_05140"
     CDS             6425..>6722
                     /locus_tag="PKN80_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35435.1"
                     /translation="MKGLTVLFLLLLLFATGCGRKSDQASSDRLQGPASTLKEYGGVM
                     GNALKKARSIDVLVPLRQTIETFKVQEGRYPASLEELVEKKYLPEMVKPPAGSRL"
BASE COUNT         1202 a         2051 c         2397 g         1072 t
ORIGIN      
        1 gaacgggcgg gccgcttcac ggtcgaggag attatcgccg ggttgaccgc caagctgaag
       61 cggcgccacc cgcacgtctt cgggacggag cgggccggtt cggcccgggc cgccctgttc
      121 agttaccgcc gggccaagcg gcttgaaaaa aagacggccg actccggcgc ggcaagcagg
      181 cggggccggc ggcccgggat ttagatccgg aagagggcgc ccagtttgcg gccgagcagg
      241 gagttggcgg ccaggatggt tacggtcagg aagagcatcg cccagacgcc gagggcggcg
      301 gcgacgtagc ggccggtccc caggatctgg aagagttcgt agagggcctt ggtaatcggg
      361 aagaaggcct gtttctgggc cagcatcaag gaatcggaga cctccagcat cgcgaagctg
      421 aaggcgaaga ggccgccggc gatcaggttg gcgctgatca ggggcagggt gatccggcgc
      481 agggtgtgcc aggtcgacgc gcccaggttt tgggccgatt cttccagcgc ttccgccgtc
      541 tgctggaggc cggcggttac cgagcgcacc acgtagggca ggcgtcggac cgcgtaagcg
      601 atgatcagca ggagcgtcgg gttgttggcc ggattgaagg cggcgaagag gcggcccggc
      661 tggctgatgg cgatgtagcc gaaggccagc acgatacccg gcaccgccag cggcagcatg
      721 gccagcaggt caagcagacc ccgtccccgg aaatttcccc gcaccaccag gtaagcgatc
      781 gccactccca gtaccatgtc gagtgtcagc gccagcgtcg cgtaatagag gctgttcttg
      841 atggacggca gggtgagagc gtggccgagc gcctcccggt agtggcccag cgtccaggcc
      901 gagggaaggg ccgactggta ccagccccgg ctgaagctgg tcagtatgac gcccaggtgg
      961 gggagggcgg cggccaggaa taccagcagg aaaaggccgg aggccagcca ggccggtccg
     1021 ccggtcagcc gccgggtttc ggcggccccg gccgacttgc ccagcatggc cgccgattgg
     1081 cgtccaaaga gaaacttgcc ggccgcgtag ataaggatcg aggtgatcat catgaaggtt
     1141 accagcgcgt agggcagcgg gttgtcgccg atgtccttga tgccgtcata gatctggacc
     1201 gcggtcaccc gggtgtaatc gaacatcagc ggagtgccca gctcggtgaa ggcccagatg
     1261 aataccagcg agccgccggc gaagaggccc ggcgccgcca acggcagggt gatgcgccag
     1321 aagcgtcgcc cgccggacgc ccccaggttt tcggccgcct cctcgagggc ggggtcgata
     1381 ttggccaggg cggcgctggc gttcaggtag aagatgggat agaggtggag cgcctgggtg
     1441 gccgccaccg cccagaagcg tccctggccc agccagtcga ccggccgcga ccagtccagg
     1501 aatccgagct ggaccagcag ggcgttcaag gcgccgaatc ggccgagcat ctggctgatg
     1561 ccgatggccc cgacgaaggg cggcagtacc atcgggacca gcaccaggcc gcccaggagc
     1621 cgcttggcgg ggaagtcgca gcgggccgcc aggaaggcca tcggcagccc gagcgcggcc
     1681 accagcaggg tggtggccag ggccagccgg aagctgttgc ttacgccttc gcggtaaatg
     1741 gggttgcgga atatttccag gaagaattcc ggggtgaatt tccccccgtg caggaagccg
     1801 cccttgagcg cctggaagag cggtccgaag aagaaaagaa cgaagaaggc cagcgtgagg
     1861 ccgaggataa gccaggtcca gccgcgccgg atcacggccg cctccggtcg gcggccgccc
     1921 gggccagcgt ttcggcctgc cggtagtttt cccggaagaa gacggtccac tcgcgggtga
     1981 tggcgatctc gtttttcttc tcgcccaggg cggcggtcgt ctcccggagc gcggccgggt
     2041 attcggcgtt ggccggcagt tgttcgagcc gggccatcgc ctccgggcag gccgacggtc
     2101 cgccggccgc ctggatggcc cggccggccg cgaccagttc cgggtgggtg tcaatgcaca
     2161 tcgcccggac cagcagccgg atcaggccga acatcggacc ggtccagccg ggccggtaga
     2221 cgaaactgcc cgcctgcagg taaggattga cgtccgggtc ggagcggtat ggccggccgg
     2281 cctctccgta agcgtccttg cggatgggca gccggcgcag tgagtacttg accggtccgc
     2341 ccggggttcc gatccggtaa ttccagaggc gctgtccctc cgggctgagc aggaattcga
     2401 cgaagcgccg ggccagttcc gcgtccgggg cgcccctgag gatgccgacc gggtcggctg
     2461 aaaaggagga tccgccggtc ggagtaaagt agcgcatccg tttcgaaccc tcctcccgct
     2521 cgacggtttc gctctggaaa cgtccgtaga aatcgatgca catgccggcg gcggcatttc
     2581 cctgggcgac gtcgagcggc accttgctgg ccgaatcggt gaagtagcgg cagttggccc
     2641 cgattcgctt gagcaggtta agcgccgtcc gccagccctc ggccagttct tcggctccgg
     2701 cccggccggg atcgaggccg gcccgggccg ccgaggccgc catctgctcc tggatcagcg
     2761 tttcaaaggc cttgttgatc gaaccgctct tggagggatc ggccagggcg atcctgccga
     2821 agaactcggg gcgggccagg tcttcccacc gctccggcgc ggccgtgccg agcgacctga
     2881 ccaggtcctc gttgtagcag attccgaagc cggagaggca gcagccgtac cagaggtctt
     2941 cggaatcgtg aagggtttcg cccgagaact gctggggcat gatctccggg ttcagccact
     3001 ccgggtgccg ggtccggatg gcgcagggct ggaggtgccc cttgcgggcc tggcgggcat
     3061 ggtcgaagga gccgcctccg aagaagacgt caatgccgat gccttcccgg ccgacccggc
     3121 cggccgcttc aaactcgctg tcgatgaacc gcacgatctc gctggtaccg cccaggttgc
     3181 gccattcgat gtccgctgtt tcgccgtacc gctgccgata ccagccgcgg aaggcgtttt
     3241 cgaattcgta gcgggtggcc tcgttatgcg ggctgacggc aaccaggcgc cgggctccgg
     3301 ccggattgct ttcggccggc ggcttgagca ggaaaggcag ggccaggatc aggaggaaag
     3361 ccgccagcca gccgtagaga cgtttcatca tccggtcagg ccgccactcc ggccagtacc
     3421 accacctcgg ccgggtccac ccagaaatcg agccggcttc cggcggcggt ctcggaattt
     3481 tccgggttga ggcggcaggc ccgcagccgg atcccggcgg cgggttccag caggtattcg
     3541 atcgattcgc ccaggtagac gctttccacc agacgggcct ctttccggtt gacgcttccg
     3601 gccgggggcg cgttgaaatt aatggcttcg ggccggatgg agagggtaac ccggtcgccg
     3661 gcctggaagc ggccggccgg gttcggtccg gtccaggttc cggccgcggt ttccacggta
     3721 agatttccgc cgccggtttc ggtcacccgg ccgggcacca ggttggtggc gccgatgaag
     3781 tcggccgtga agagactggc cggctttcgg tacaactcct ggggggaacc gatctgctcg
     3841 acccggccga ggttgagtac cgcgcagcgg tcggccatgc tgagcgcctc cttctggtcg
     3901 tgggtgacgt agagagcggt caggccggtc tcgcgcacga tccgcttcag ttccgtccgc
     3961 atctcgcggc gcagcttggc gtccaggttg gaaaggggtt cgtccagcag gaggcagtcg
     4021 ggcttgatca ccagcgcgcg cgccagcgcc acccgctgct gctggccgcc ggagagctgg
     4081 ccgggccgcc ggccggcgta attttcaagg tggaccatgg ccagggcggc ggtcacccgt
     4141 tctccgcgtt ctttcggagg caggttgcgg ccgggtacgg taagaccgaa ggccacgttc
     4201 tgggccaccg taaggtgcgg ccagagcgcg taattctgga agaccatgcc ggtgttgcgg
     4261 cggtaaggcg gcagatcatt caccagccgg ttgtcaaaga ggatgcggcc ggaatcggtc
     4321 ttcacgaaac cggccaccgc ccgcagcagg gtggtcttgc cgcagccgga ggggccgaga
     4381 atgaagaaga actcgccatc ctcaacctgg aaggagacgt cccgcagggc ggcggtcctt
     4441 ccgaaattct tgttgatttt ttcaatcgtg atcttcatgg gcgggccatc cgggcggtcc
     4501 ggttcggact gcgtccgggc ggttcggcgg accggtaaag ggtgaagcga cggctccgac
     4561 tgtcaatggt ggtgttcggt cagttcccgg gccagctccg gaagccgggc aaggtccgcg
     4621 acctcctgga gctcgtccat cacggtgccg tgagcgtcca ggacccggat gagcgggagc
     4681 ttggtttcgg ccgtgatccc gggcatcgcc ggaccggtgg ttgtcagggt aatcgccttg
     4741 ccgcccagtt tcggccgggc ttctcgaagt gccttgacga aggggcggtc ccgctcggtt
     4801 tggctcttga ccagcaggat tacgcccagg tggtcgcccg ccgcattctg gagttgttca
     4861 atgatggatt cccgctgggt gagggcgccg agcaggtaaa gggcgccgaa ggcgaggata
     4921 agcgcgaggg ccttctggta ccagttaagt tttttggtcg gcattctttt tctcccggaa
     4981 agttttttat ccttatattt taaaaaccgc tcgcctggtt gtcaaaccag cggtcttcat
     5041 tttatcctcg ggtttcgtcc gggcggcgcc gggagcgggc aaaaaggagc ccctggccgg
     5101 ccagcagaag aagaaagaag gaaagcatcg ccgcgtgaaa gacgtccggg ccccggtcca
     5161 gggccgcgcc gccggccagg ggacccagga cgccggcgac gcccacgatc gcctcgttga
     5221 tgccgatgtt gcggccgctc tgctcggtct ggctcaaggc gtagaagacc ccggccgagt
     5281 aagcgtagcc gcagtagagt ccgaaaccgg cggccagggt cagcattccc ggccagccgg
     5341 gccagagcca ggcggccagc aggccggcgc cgccggccag gctggtggcc aggatggggc
     5401 ggaacccgta attcaaggtg tgccggccgg agagcaggaa accggagacg gcctggatgg
     5461 agaagaccag gcagacaagg gttccggccg ccgccggccc ggaaccgggc aggtccagga
     5521 tcagcttggg taccagtatc cggatggcga tgccgataaa ggaggcgttg agaaagagca
     5581 gccagccaag ccggacgaag agggcacgcc gggaagccgg aacgggcggc ccgggtggct
     5641 ccgccgggtc gggaccggtc ggaccgggcc ggatcggcca gtgccggtct atctccagga
     5701 ccaggatgac cagcagggac gagaggaccg ccggcatcag cgccgccacc gggccgcgcg
     5761 tcaccaggta gccctgggcc agcgaaccgg cgccgatgcc caggctccag ctcagggtgt
     5821 agaagccgca ggcctggtgg atcggcagcc tgacccgttc ggccataaag agctggaaac
     5881 cgatgtagaa caatgaggcc gccaccccgt agagggcgaa gaagaagaat aacagggcga
     5941 ggcggtcggc ccacatcagg ccgacggtca ccaggaccat gagcaggcag ccggcgatca
     6001 tcgaagcgta gcggttggaa gcgttgatca gccggccgag cagcagcgag accaggaagt
     6061 agaccagtcc ccagacggcg cccagggcgc ccagcaggaa ggcgctggcc cccatttcgg
     6121 ccgccttgag cacgccgaag agactgaggg cggccagcga gaagtccatc agggccggga
     6181 agaggtaaat ccatcggccc cggccgtttc cgaattttat catcagcggt ttccgcgcgc
     6241 gtttatcgtt gaaattccgg tcgatttcaa ataaacgttt agtatagcat ttagttccgg
     6301 ggccgggaaa cgaagccgct tgcccggccc gatcgaatcc ggcggcgttt gtatcccggc
     6361 gatcttatga tataaaatta accagccagg agcggcgggg tgttttgaaa agacgggagg
     6421 gggtatgaaa gggttgacgg ttctctttct gctgctgttg ctcttcgcaa ccgggtgcgg
     6481 ccgcaagtcg gaccaggcgt cttccgaccg gctccagggt ccggccagca ccttgaaaga
     6541 gtacggcgga gtcatgggca atgccttgaa gaaggcccgg tcaattgacg tgctggttcc
     6601 gctcaggcag acgatcgaga ccttcaaggt ccaggagggg cgttacccgg ccagcctcga
     6661 ggagctggtc gaaaagaagt acctgcccga aatggtcaag ccgccggccg gcagccgcct
     6721 gg
//
LOCUS       DAOQYN010000101         6701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_11790_length_6701_cov_5.015949, whole genome shotgun sequence.
ACCESSION   DAOQYN010000101 DAOQYN010000000
VERSION     DAOQYN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11790_length_6701_cov_5.015949"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2041
                     /locus_tag="PKN80_05145"
     CDS             <1..2041
                     /locus_tag="PKN80_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35436.1"
                     /translation="KLTCLRPELARVTINFNWNDHAPDLTVSLEVASRAAAAAELGIR
                     AFCLDLACETIRARLLPGLYSGWSCEGRSLVVFPVYEHGRPRQLGLVCDSQPLAAGDI
                     RTRRQGLALNLPTGLFNGVALMDPGETWSGGLVIRFGADDIWDPALARPAAAFLKTAV
                     GIQPPRHSYQTHITRWERLLKQPELWLSLGPGLGMYHRGFYGMIRFGETRVSGPLLYR
                     GYQGQGTSQEHLVELAWGGSSNALLAYTMAHYPADWSRQRARQIAAAAVRFKGNGFQA
                     GEGPCRGAWYNGYLWEMDRFSDRYGRESIYTPDQGITNYFLGKLLLEKTLPVPGLAEK
                     IRTNCFEYLRSLRNRQGGLENARKFDGSPGVDRYELPYDGHKADLPMASAMGALSFLI
                     CYQLTGEAAAREEGQALLDHLREKIAANEWRFHEYDTWGWDNMAMCWILVTLCEWQAA
                     FPEMDLAAPIEKTLAALLSCQHRFDFELDRYSQLERCWGGLMVNRGGWAVGTTVGSLQ
                     AGKGLHNRFDTALALWTHFRLTGDTRSYTALLEFLNYLTYHQWTRNDLPLGPGAVTEA
                     MHLNEGHIQDTVQTLHSNCLHHILLNRGLFLESASLEPDRVKLPAPNGTGSQLRFSFR
                     PGKAGPVRFSIDGAGSDNIELKLDGQVVYRGPGRNLNWFLPEAGRTWEFTAESVK"
     gene            2050..3303
                     /locus_tag="PKN80_05150"
     CDS             2050..3303
                     /locus_tag="PKN80_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNQ35437.1"
                     /translation="MPSLARCPGCGTEFRDASGSPGLTCPNCRLEFPRPLARSRERIR
                     LPRYPFAYQDLATPAVADFRRRYRLDRVIAGARDEFEALTAIRDWTFRRFPRGTPVHQ
                     QLDAEAILAAGRKGGTFHCTYYAFVSVQACTALGHVARRLNIDRPQPAPGRFAHMVYD
                     VWSNQLDQWVVMDSKANIHFELDDKPLSALELRRAWHEGRGGRIRAVFGHGREKRLLP
                     KDRSELSAWGNAYYSKLWKRKETVWGPQLIEMTDPANYGFIGVYGAADFFSRPAAPNF
                     VKHFPVLLWYDRWNGGERWLCDKKNPASPEAYRLEGLLKPVRRRQDLEWPVNRTEIQL
                     LPATPAGWPVTLVTCTPNFSRFEFRLDGGSWRQARLSNGPANLKEIPVLHLPAEAGRV
                     LEVSSVNLGGVRGSADRVRLPVARD"
     gene            3313..5277
                     /locus_tag="PKN80_05155"
     CDS             3313..5277
                     /locus_tag="PKN80_05155"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNQ35438.1"
                     /translation="MLKIELDNLIDWRQIPDLPPGANRPQRLATLFGHLASRKKPAYL
                     FEAADRKRLIEELLPASGHPVEFELTKARILMRPHLQEHPHRRYFQTNGEIDWELELP
                     YELRRHIYLQPLASHYLLTGEAAALEKGAALVRAWWQNAPVNRQERQAGALKPSLERS
                     RSLEAFLRFFAASRDWPGWQRELSWFLPMMYALAEVTRRSERLVFHNIFYTSLRMRHA
                     FALYFPEFKASKAWEAETLVDMVASLKEAVRTDGGQIEQSPGYHAGVMDGTADLVILR
                     RRNRRPVPAAITDRLRRMAEFIEGLADPDGGLPCLSDTGYGRQAARPLLFKAAAALGS
                     TRRFAGSSRYAHLFSLPPELVLKTAVRPERCRPTFAFFPESGYYAMRSGPEPEALYLV
                     FDAGPHGYFHGHLDLFNIELRAGGQQLVPDPGTYHYSISPARLGQISTPVHNTINLNI
                     FSHRPYEDGEPPLARILQARHYPDNSVAITALHRAFDHLWGEPVVTRQIFYDGRNRWV
                     FVDRLECRKPNPIKRQHAFYVAFNLPDSRRYRFQPEAGEVRTTFEDRPNLRLRVYAPE
                     KLSHLILHPGSYLSPDGQRDTWRWSAVINADQACLVHLLEVEPGTGKNESRFEVEEDG
                     EKISVRFGPGSSPRRVIFTPKHVRVEKRSG"
     gene            5298..6626
                     /locus_tag="PKN80_05160"
     CDS             5298..6626
                     /locus_tag="PKN80_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35439.1"
                     /translation="MKPKLFTNGRFCTPQGIRPNLSLEVRGGRIAGLFPVGRGNRKPV
                     ETIDLQGRYLLPGFLEIHTHGAGGFELTAGRYRPERGAFESSGRIYRQELPLYCRRRA
                     STGITGLYLGTLAAPVKRLQFVFRQLADYMESAGNGRDGCLVHGGLLEGTFMLPEFCG
                     AHNTDYIYPPDIELFKRINESEIIRLTNVVPDSGTPALKLIRHLARRGISVGAGHTNA
                     SHRQFQLARRAGLKYIIHLLNGPTGHSYKVFEGGGALEAALAEPFHVEIIADGIHVNP
                     LYVRDILARKGPGKVMLVTDSMFPSQAGLINHFNVSGIAGGINHKGRYAYVIGKKPIT
                     LFSTLITMDQAFGNLLSWLTVEGPGIWHEKHPARPFNEALAIVSRCCSANIAAMLKEG
                     GGDDLETGELAAGRWADLVAAEITGRPGRYRLRVREVYVRGRRVWPEPEA"
BASE COUNT         1199 a         2352 c         2048 g         1102 t
ORIGIN      
        1 aaaactaacc tgcttacggc cggaactggc ccgggtcacg atcaacttca actggaacga
       61 ccacgccccg gacctgaccg tcagccttga agtcgcctcc cgcgccgcgg ccgccgcgga
      121 actgggcatc cgcgccttct gcctggacct ggcctgcgaa accatccggg cccggctgct
      181 gcccggcctg tattccggct ggtcatgcga aggccgttcc ctggtcgtct ttcccgttta
      241 cgaacacggc cggccccgcc agctgggcct ggtctgcgac agccagcccc tggccgccgg
      301 cgacatccgg acccgccggc agggactggc cctgaacctt ccgaccggcc tcttcaacgg
      361 ggtggcgctg atggacccgg gcgaaacctg gtcgggcggc ctcgtaatcc gtttcggcgc
      421 cgacgacatc tgggacccgg ccctggcccg gccggccgcc gccttcctga aaacggcggt
      481 cggcatccag ccgccccgcc attcctacca gacccacatc acgcgctggg agcggctcct
      541 gaaacagccg gagttatggc tttccctggg gccggggctg ggcatgtacc accggggctt
      601 ttacggcatg atccgtttcg gcgagacgcg ggtaagcgga ccgcttcttt accggggcta
      661 ccagggccag ggaacctccc aggaacacct ggtcgaactg gcctggggcg gcagcagcaa
      721 cgctctgctg gcctacacga tggcccacta cccggccgac tggtcccggc agcgggcccg
      781 ccagatcgcc gccgcggccg tccgcttcaa gggtaacggt ttccaggccg gggagggacc
      841 ctgccggggc gcctggtaca acgggtacct ctgggaaatg gaccggttca gcgaccgcta
      901 cgggcgggag agcatctaca cgcccgacca gggcatcacc aactacttcc tgggcaagct
      961 gctgctggag aaaaccctgc cggttccggg cctggccgaa aagataagga ccaactgctt
     1021 tgaatacctt cgctcgctgc gcaaccggca gggcggcctg gagaatgccc ggaaatttga
     1081 cggcagtccg ggcgtcgacc gctacgaact tccctacgac gggcacaagg cggaccttcc
     1141 gatggccagc gccatggggg cgctctcctt cctgatctgc tatcagttga ccggggaggc
     1201 ggccgcccgg gaggaggggc aggccctgct ggaccacctg cgggagaaga tcgcggccaa
     1261 cgagtggcgc ttccacgagt acgacacctg gggctgggat aacatggcca tgtgctggat
     1321 cctggtcact ctctgcgaat ggcaggccgc cttccccgaa atggatctgg cggccccgat
     1381 cgaaaagacc ctggccgcgc tcctctcctg ccagcaccgg ttcgacttcg aactcgaccg
     1441 ttactcccag ctggaacgct gctggggcgg cctgatggtc aaccggggcg gctgggcggt
     1501 gggcaccacg gtcggcagtc tccaggccgg caagggcctg cacaaccggt tcgacacggc
     1561 cctggctctc tggacccact tccggctcac cggcgatacc agatcctaca ccgccctgct
     1621 ggagtttctc aattatctca cctaccacca gtggacccgg aacgacctgc cgctcgggcc
     1681 gggcgcggtc accgaggcaa tgcacctgaa cgagggccat atccaggaca ccgtccagac
     1741 gctccattcc aactgcctgc accatatatt gctcaaccgc ggcctcttcc tcgaatcggc
     1801 ctcgctcgaa ccggaccggg tgaaactgcc ggctcccaac gggacgggga gtcagctccg
     1861 cttctcgttc cggcccggaa aggcgggccc ggtccgcttc agcatcgacg gcgccggttc
     1921 cgacaacatc gaattgaagc tggacggaca ggtcgtctac cgcggcccgg ggcggaatct
     1981 taactggttc ctgcccgaag ccggccggac ctgggaattc acggcggaat cggtaaagta
     2041 ggaatgaaca tgccgtcctt ggcgcgctgc ccgggctgcg gaacggaatt ccgggatgcc
     2101 tccggaagtc ccggccttac ctgtccgaac tgccggctgg aattcccgcg gccgctggcc
     2161 cggagccggg aacggatccg gctgccccgc tacccgttcg cttaccagga cctggccacc
     2221 ccggccgtgg ccgatttccg gcggcgctac cggctcgacc gggtgattgc cggcgcccgg
     2281 gatgaattcg aggcgctgac ggccataagg gactggacct tccgccgctt tccccgcggg
     2341 acacccgtcc accagcagct tgacgccgag gccatcctgg ccgccggccg gaagggcggc
     2401 acctttcact gcacttacta cgccttcgtc tctgtccagg cctgcaccgc cctgggccac
     2461 gtcgcccggc gccttaatat agaccgtccc cagccggcgc cgggccgctt cgcccacatg
     2521 gtctacgacg tctggtcaaa ccagctggac caatgggtgg tgatggactc caaggccaac
     2581 atccatttcg aactggacga taaaccgctc tcggccctgg aactgcgccg ggcttggcac
     2641 gaagggcgcg gcggccggat ccgggccgtc ttcggccacg gccgagaaaa acgcctcctg
     2701 ccgaaggacc gctcggaact gtccgcctgg ggaaacgcct actactcgaa actctggaag
     2761 cggaaggaga ccgtctgggg tccccagctg attgaaatga ccgacccggc caattacggg
     2821 ttcatcgggg tttacggcgc cgccgacttc ttctcccggc cggccgcgcc gaatttcgtc
     2881 aagcactttc cggtcctgct ctggtacgac cgctggaatg gcggggaacg ctggctctgc
     2941 gacaagaaga accccgcctc gccggaggcc taccgccttg agggcctcct caagccggtg
     3001 cggcgccgcc aggacctgga atggccggtc aaccggaccg agatccagct cctgcccgcc
     3061 accccggccg gctggccggt caccctggtc acctgcacgc ccaacttcag ccgcttcgag
     3121 ttccgcctcg atggcggatc ctggcgccag gcgcggctgt caaacggacc ggcgaacctg
     3181 aaggaaatcc cggttctcca tctcccggcc gaagccggcc gcgtcctgga ggtcagcagc
     3241 gtcaacctcg gcggagtccg cggatcggcg gaccgggtgc gcctgcccgt agcaagagat
     3301 tgaggagccc gaatgctgaa aatagaactg gataacctga tcgactggcg gcaaataccc
     3361 gatctccccc ccggggcaaa ccggccgcag cggctggcaa ccctcttcgg acacctggcc
     3421 agccgaaaaa aacccgccta tctctttgaa gccgccgacc ggaaacgatt aatcgaagaa
     3481 ctgctgccgg ccagcggcca cccggtggag ttcgagctca ccaaggcccg gatcctgatg
     3541 cgcccccatc tccaggagca tccccaccgg cgctacttcc agacgaacgg ggagattgac
     3601 tgggaactgg aacttcccta cgaattgagg cgccatatct acctgcagcc gctggccagc
     3661 cattacctgc tgaccggcga ggcggccgcc ctcgaaaagg gggccgccct ggtccgcgcc
     3721 tggtggcaga acgccccggt caaccggcag gaacgccagg ccggggccct gaaaccctcg
     3781 ctggagcgga gccggtcgct ggaggccttc ctgcgcttct tcgccgccag ccgggactgg
     3841 ccgggctggc aacgggaact gagctggttc ctgccgatga tgtacgccct ggccgaggtg
     3901 acgcgccgga gcgaacgcct ggtctttcac aatatcttct acaccagtct gcggatgcgc
     3961 cacgccttcg ccctttactt cccggaattc aaggcctcga aggcctggga ggccgagacc
     4021 ctggttgaca tggttgcctc gctcaaggag gccgtccgga ccgacggcgg ccagattgaa
     4081 cagagtcccg gctaccacgc cggggtaatg gacggcaccg ccgacctggt catcctgagg
     4141 cggcgcaacc gtcggccggt cccggcggcc atcaccgatc ggctccggag aatggccgaa
     4201 ttcatcgagg gcctggccga cccggacggc ggcctgccct gcctgagcga caccgggtac
     4261 ggccgccagg cggcccggcc gctgctcttc aaggcggcgg ccgccctcgg atcgacccgg
     4321 cgttttgccg gatccagccg ctacgcccac ctcttctcac tgccgcccga actggtgctg
     4381 aagaccgccg tccgtccgga gcgctgccgg ccgaccttcg ccttctttcc cgagagcggc
     4441 tactacgcca tgcgttccgg tccggaaccg gaggcccttt acctggtctt cgacgccggg
     4501 ccgcacggtt actttcacgg ccacctggac ctcttcaaca ttgagctgcg cgccggaggt
     4561 caacaactgg tacccgaccc ggggacctac cattattcca taagcccggc ccgcctgggc
     4621 cagatctcga ccccggttca caacaccatc aacctgaaca ttttcagcca ccgtccttac
     4681 gaggacggcg aaccgccact ggcgcggatc ctgcaggccc gccattaccc ggacaactcg
     4741 gtggccatca ccgccctcca ccgggccttc gatcacctct ggggcgagcc ggtcgtcacc
     4801 cggcagatct tttatgacgg ccggaaccgt tgggtcttcg tcgaccggct cgaatgccgg
     4861 aaaccaaatc ccatcaagcg ccagcatgcc ttttatgtcg ccttcaacct gcccgacagc
     4921 cgccgctacc gcttccagcc ggaggccgga gaggtacgga cgaccttcga agaccggccc
     4981 aacctgcggc tcagggttta cgcaccggaa aaactctccc acctgattct tcatcccggt
     5041 tcctacctga gcccggacgg acagcgggat acctggcgct ggagcgcggt gatcaatgcc
     5101 gaccaggcct gcctggtcca ccttctcgag gttgaacccg gcaccgggaa aaatgaatcg
     5161 cgtttcgagg tggaggagga cggggagaaa atctcggtcc gcttcggtcc cggatcatct
     5221 ccgcggcggg ttattttcac cccgaaacac gtccgggtcg aaaaacggtc cggatgaagg
     5281 ccgaaacaag agcccccgtg aaaccaaaac tttttaccaa cggccgattc tgcaccccgc
     5341 aaggcatccg gccgaatctg tccctggagg tacgtggcgg ccggatcgcc ggacttttcc
     5401 cggtcggccg cggcaaccga aaaccggtcg aaacgattga cctgcaaggc cgctacctgc
     5461 tgcccggatt cctggagatt cacacccacg gcgccggcgg gtttgaatta accgccggcc
     5521 ggtatcggcc ggaacgggga gccttcgaat cctcagggag aatttatcgg caggaactcc
     5581 cgctttactg ccggcgccgg gcctccaccg gcatcaccgg cctctacctg ggaaccctgg
     5641 cggcgccggt caagcggctc cagttcgtct tccgccagct ggccgattac atggaatccg
     5701 ccggcaacgg ccgggacggc tgcctggtac acggcggact gctggaagga actttcatgc
     5761 ttccggaatt ctgcggcgcc cacaacaccg actacatcta tccgcccgac atcgagctct
     5821 tcaagcgcat caacgaatca gaaatcatcc ggctgaccaa cgtcgtcccc gattccggga
     5881 cgccggccct gaagctgatc cgtcacctgg cccgccgcgg catctcggtc ggcgccggcc
     5941 ataccaatgc cagccaccgg cagttccagc tggcccgccg ggcgggattg aagtacatca
     6001 tccacctgct caacggaccc accggccact cctacaaggt cttcgagggc ggcggcgccc
     6061 tggaggcggc cctggccgaa cctttccacg tcgagatcat cgccgacggg atccacgtca
     6121 atcccctgta cgtgcgcgac atcctggccc ggaagggtcc cgggaaagtg atgctggtga
     6181 ccgattcgat gttcccttcc caagccggcc tgattaatca cttcaatgta agcgggatag
     6241 cgggcggcat caatcacaag ggccgctatg cctatgtaat cggtaaaaaa cccatcacgc
     6301 ttttttccac cctgatcacc atggaccagg ccttcggcaa tcttctctcc tggctgaccg
     6361 tcgaagggcc ggggatctgg catgaaaaac atccggcccg ccccttcaac gaggcgctgg
     6421 ccatcgtcag ccgctgctgt tcggccaaca tcgcggcgat gttaaaggaa ggcggcgggg
     6481 atgacctgga gaccggggaa ctggccgccg gccgctgggc cgacctggtg gccgccgaga
     6541 tcaccggccg cccgggccga taccggctcc gggtccggga ggtctacgtc cggggaaggc
     6601 gggtctggcc ggagccggag gcttgatggc cgaaaaccgg gtttgcacgg acctgaacgg
     6661 caagtggtgg attttctttg accgggagga cctgggcatc a
//
LOCUS       DAOQYN010000102         6671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_11853_length_6671_cov_5.950574, whole genome shotgun sequence.
ACCESSION   DAOQYN010000102 DAOQYN010000000
VERSION     DAOQYN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11853_length_6671_cov_5.950574"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PKN80_05165"
     CDS             complement(<1..808)
                     /locus_tag="PKN80_05165"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35440.1"
                     /translation="MADFETSVRYLKGVGEQRAHLLEKIGVRILFDLVYYFPRRYEDR
                     KKITPVAETVPGQPATICATVLTSGSKSGWRGKQIFTAALSDDTGLIYATFFNQGYLK
                     DVLVPGCRVLLSGRPSIYQGEKSMIHPVFEVLKEGTGRPTEAGRIVALYRPPEGLSQR
                     AFHLIMLSALRELGSEVKEVVPFAERQRLNLGNLRHALKNIHFPVSETDLRKAREYLI
                     FEEFFLLAAALRKRRLDSRGVAGGPGPEGPAGGDPAAFISGFQALLSFPLP"
     gene            complement(842..1615)
                     /locus_tag="PKN80_05170"
     CDS             complement(842..1615)
                     /locus_tag="PKN80_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNQ35441.1"
                     /translation="MGFLVFLFGAVFGSFLNVCIYRMPLSLSIVKPNSFCPGCKKPIK
                     WFDNIPMLSYLMLSGRCRHCRKPISPRYFLVELLTAALFLFLYWQFGLDVRFFVFAPF
                     VMALILVSFIDWDHYLIPDCIVLPGIPIGLLLNFFFPVLAGLNAWLPALRDAFIGVLV
                     GGGFLLLLGWLGTLAFKKDAMGGGDVKLLAMIGAFLGWKSVLMTLFFGSLFGSVVSLA
                     LIAAGVKKRDEYIPFGPYLSLGAVLALFFRGGTFLGFPV"
     gene            complement(1702..1773)
                     /locus_tag="PKN80_05175"
     tRNA            complement(1702..1773)
                     /locus_tag="PKN80_05175"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1738..1740),aa:Glu,seq:ttc)
     gene            complement(1777..2217)
                     /locus_tag="PKN80_05180"
     CDS             complement(1777..2217)
                     /locus_tag="PKN80_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35442.1"
                     /translation="MTLFSRVRVAINLLLLIAAVAGLAAARVYRERYLREKAARVQSA
                     RSSADYFLSGAQEQLLLAAMSVKRDNFAAAAHQVREARRGLEAFRSAAGEAGPPDLLG
                     PLAAIEAGIAGVRPAVQDLILDLAGEISEFRSDLSGPEPARPGS"
     gene            3523..4785
                     /locus_tag="PKN80_05185"
     CDS             3523..4785
                     /locus_tag="PKN80_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35443.1"
                     /translation="MFNNILRALGADKTREEKASEVNNKALEYAKKGDRETAYRLYLK
                     ALSLNPDDQTIRRNISITITELGRKAYVDKNYRLAAQKYEEALLYDPDNQTLRKYLQS
                     ARELLADETRLAGVKPKLEKMLDQFAAEAGGKSPVEIGAAVSGGTRSGTKTGFGDASV
                     VDLTFLDPNKPISVDPHVVKGQPRVFLVQPGTCTPKGEDYDKGFEELKKHNPAAALEF
                     FKRAETENPDNPLVRNAMDLTQHMLKTREAAARTREEEERWVNGRMAFLNATEVLRKG
                     DVANADALLYEASKTNPGDSEIREISRMMGMYLYWAKKGQPAAAEPDERKKLHAAVAE
                     RSGAAMVYILRDQYEAAVAELDAADALFAKQVDLEMLAEGLSLKNPPSETEKEGHKND
                     AMMRSGLADARRELIRLLEKKSREKPAE"
     gene            4832..6667
                     /locus_tag="PKN80_05190"
     CDS             4832..6667
                     /locus_tag="PKN80_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35444.1"
                     /translation="MKFFKIILAGLFCLCLLPAPAAGAESRDPSIAAGQAALKQGMAA
                     AGKKNWPLAVKHYTEAQRRLHLSPQVMYNLALAHAQAGNELLADVWFRAYLGAVPQAA
                     NADQVRTEISRLEQAADEKVQRLFKQAEDLAEALPLEANNQGRGRRAEAFKSIANIRI
                     AVGDFKRAEEDLLKMNAYFSGKNVPDRYYPDANTRYADFAGTLAEAGDVEGARRLRGQ
                     LKNGDGLAMPILMALVEAGDLKEAKEFLEDEKETRFGVRLEPYETALRAFAEKGDLAT
                     AEKIIGAARNYSQDYPEYLHLELAHLLRDQGDLTGAQRYARLTDNYRTAIAIKSDIEE
                     SLRGLENSGVPVFQPYYAVETIADIVKTCLWLGETRKAEKALKIGDAWIAKNRRQLGG
                     LGGDGKSVAEGYLRIGRAYLEVENGRGAEAIKLVQSISFPWEISEAAGEFIQQAYQIE
                     LKRDLANFAISRGKPAAAERIADSFAESRERIEFLRRVLALYDSKADRANTERLTKKL
                     AGLLAEVQAGGWDPENPGREFVVNAWTDAAYALVWGVHPRTFRTSSMKILEWRGAPAI
                     YNLPAYIAEIKKTSSPDAMPASFAESAKTIGKGLLRIRALDRIYR"
BASE COUNT         1535 a         2016 c         2064 g         1056 t
ORIGIN      
        1 cggggagcgg aaaggagagc agggcctgga aaccggagat aaaagcggcc gggtcgcccc
       61 cggccggacc ctccgggccc ggtccacccg ccactccccg gctgtccagc cgccgcttgc
      121 gcagggcggc cgccagcagg aagaactcct cgaagatcag gtactcccgg gccttccgga
      181 ggtccgtctc cgaaaccgga aagtggatat tcttgagggc gtggcggagg ttgcccaggt
      241 tgagccgctg ccgctcggcg aaggggacca cctctttcac ctccgacccc agttcccgca
      301 gcgccgacag catgatcagg tgaaaggcgc gctggctcaa cccctccggc ggccggtaaa
      361 gggccacgat ccggcccgcc tcggtagggc ggcccgtccc ctccttcagc acctcgaaga
      421 ccgggtgaat catgctcttc tcgccctggt agatggaggg acggcccgag aggagcaccc
      481 ggcagcccgg gaccagcacg tccttgaggt aaccctggtt gaagaaggtg gcgtaaatga
      541 ggccggtgtc gtcggagagg gcggcggtga aaatctgctt gccgcgccag ccgctcttgc
      601 tgccgctggt aagcaccgtg gcgcagatgg tggccggctg gcccggcacc gtttcggcca
      661 ccggggtgat cttcttgcgg tcctcgtaac ggcgaggaaa gtagtaaacc aggtcgaaaa
      721 ggatgcggac gccgatcttc tccagcaggt gggcccgctg ctcacccacc cccttcaggt
      781 aacgaaccga ggtttcaaaa tcggccatcg ccgcttctcc cggccgcggg cccgcccggg
      841 atcagaccgg gaaccccagg aaggtcccgc ccctgaaaaa gagcgcgagc accgcgccca
      901 gcgaaaggta gggaccgaag ggaatgtact cgtcgcgctt cttcaccccg gcggcgataa
      961 gcgccaggct gaccacgctg ccgaaaagtg aaccgaagaa gagcgtcatc agcaccgact
     1021 tccagcccag gaaggcgccg atcatggcca gcagcttcac gtcgccgccg cccatggcgt
     1081 ccttcttgaa ggccagcgtg cccagccagc ccaggagcag caggaagccg ccgccgacca
     1141 gaacgccgat aaaggcgtcc cggagggccg gaagccaggc gttcaaaccg gccaggaccg
     1201 ggaagaaaaa attcagcagc aggccgatcg ggatccccgg cagcacgatg caatcgggaa
     1261 tcaggtagtg gtcccagtcg atgaaggaga ccaggatcag ggccatcacg aagggcgcga
     1321 aaacgaagaa gcggacgtcc agcccgaact gccagtaaag aaagagaaag agggcggccg
     1381 taagcagttc gaccaggaag tagcgtgggc tgatcggctt gcggcagtgg cggcagcgcc
     1441 cggaaagcat caggtagctg agcatcggta tattgtcgaa ccacttgatc ggcttcttgc
     1501 agcccgggca gaaggagttg ggcttgacga tggagagact caagggcatc cggtagatgc
     1561 agacgttcag gaaactgccg aagaccgcgc cgaaaaggaa gaccagaaat cccattcagt
     1621 ttctccgtcc tgttcgcaaa agacaacggc ggggaaggcg ggcaaaaaac cggtccccgc
     1681 cggagcaagg cgggggagaa gcggggcgta cgggaattga acccgtgtta acggcttgaa
     1741 agaccgttgt ctttaccact ggaccaacgc cccaatttaa ctgcccggcc gggccggctc
     1801 cgggccggaa aggtcggatc gaaactctga aatttcgccg gccagatcca gaatcaggtc
     1861 ctggacggcc ggcctgaccc cggcgatccc ggcctcgatg gccgccaggg gccccaggag
     1921 atccggcggc ccggcctcac cggccgcaga ccggaaggcc tccaggccgc gccgggcctc
     1981 cctgacctgg tgcgcggctg ccgcgaagtt atcgcgcttg accgacattg ccgccaggag
     2041 caactgctcc tgggcgccgg agaggaagta atcggccgaa ctccgggccg actgaacccg
     2101 ggccgccttc tcgcggaggt agcgctcccg gtaaacccgg gccgccgcca gcccggccac
     2161 ggccgcgatc agcagcagca ggttaatcgc gacccttacc cggctgaata atgtcatatt
     2221 atttaaaaaa gaacccggga aattataacc caagccgggg cttgaaggca agaaagcgcg
     2281 gcggtcttcg gttcggggcg ccgagggtcc ggactcgact cgggcgctcc gccgcggtgg
     2341 aaaggtacgc ttaaatccgg cgcgcggcta actcgccgtc cgcttggggc cggaaggcgg
     2401 ccgtcgcggc ctggctcaga caggagccgc gggaggatat cgatgccgga ccgctgtcgc
     2461 ttttaagctt ctttcccgag gccgcggctt cacgactcgc ctcgttccgg gccctcggct
     2521 tcaggggaac agacgaaaag caaaaacgag gcaaaggcaa aactacgccg ctcgacaacg
     2581 agcctcgccg tgttcccggg cctggtaagg cggatccggg ccccggctac tcattgtctc
     2641 gcccggcttg ttttgtagcc atcgctacgc aagcggcgct caccgaaaaa cgccgcttaa
     2701 ggggaacaga cgaaaggcaa cggcgaggat ggcggaggtg gtgattaacg gccagtgttt
     2761 ccccgccgaa gccgtttgtt tgggcgccgg cggattcgtc tgaagagaga ttcagaaaca
     2821 accgggccgt gaagcagagc gaagcacgct tcacggctta aaggaaacgg acgaaaggca
     2881 agcctaaaga aaagccccgc atgcaaaacc cgccttgtaa acttgacaaa acccttttgc
     2941 agtggtataa ataaaaaacg aaagaaaaac aatgtttagg tcgttattga aaaggccttg
     3001 aatggccggc aaattacaat taaaagaagg tttttaccgg acgatcccgc gccccgactc
     3061 gcacccgctt cttcttcccc gcgccaacct gcttccagcg atgcttctcc tgacctgaca
     3121 cccggttgtg ggaccttgcc ccaactcgat aagtttgtcc ctttttcgaa gtggcgtttt
     3181 tctcaaaagg ccgtttctta tggaaagggg gaaagaatga aaatccgatc ttcagacgcc
     3241 cgaatcccgt cttcgttgat caacctttct cgtgcccttc tcttcgccgg ctgcctttta
     3301 ttgggaatca cattatttcc cgcgctcttg aaagccgagt ggtatattga agttcccagg
     3361 gccggccaaa gcaccgtcgg cacctcccgc ctcggcccct actcgacccg ggaggaggcg
     3421 gaagcggtca atcgaaatta ttttgacggt aagagatcca ttacgggctc cggaagcggc
     3481 gagtcatcct ccggcgattg ggaggattgg agcttcacga atttgttcaa caatattctc
     3541 cgggctttgg gcgccgacaa aaccagggag gagaaggcca gcgaggtcaa taacaaggcg
     3601 ctggaatatg ccaagaaagg cgaccgggaa accgcctacc ggctttatct gaaggccctg
     3661 agcctgaatc ccgacgacca gacgattcgc aggaacatct ccataacgat caccgaactg
     3721 ggacgcaagg cctatgtaga taaaaattac cgcctggcgg cccagaaata tgaagaagcg
     3781 ctgctctatg acccggataa ccagaccctc cggaaatatc tccaatccgc ccgggaactc
     3841 ctggccgacg aaacccggct cgccggcgtg aaaccgaaac tggaaaagat gctcgaccag
     3901 ttcgccgccg aagccggcgg caagagtccg gtcgaaatcg gggcggccgt cagcggcgga
     3961 acgaggtccg gaacgaaaac cggcttcggc gacgcatcgg tagtggacct cacctttctc
     4021 gaccccaaca aacccatctc ggttgatccg catgtggtca agggccagcc gcgggtcttt
     4081 ctggttcaac cggggacctg cacgccgaag ggcgaggact acgacaaggg tttcgaggaa
     4141 ttgaagaagc acaacccggc cgccgccctc gagttcttca agcgggctga aacggagaat
     4201 cccgacaacc cgctggtgcg caatgccatg gacctcaccc agcacatgct caagacgcgc
     4261 gaggcggccg cccggacccg agaagaagag gaacggtggg tcaacggccg gatggccttc
     4321 ctcaacgcga ccgaggtgct ccgcaagggg gacgtcgcaa atgcggacgc gctcctttac
     4381 gaggcgtcca aaacgaatcc gggcgacagc gaaatccggg aaatatctcg gatgatgggg
     4441 atgtaccttt actgggccaa gaaaggccaa ccggccgccg ccgaaccgga tgaacgaaag
     4501 aagctccacg ccgccgtggc cgaacgcagc ggcgcggcaa tggtctacat tttgagagat
     4561 cagtacgagg cggcggtagc cgaacttgac gccgccgacg ctctctttgc caaacaggtt
     4621 gatcttgaaa tgctggccga gggactctcg ctgaagaacc cgccgagcga aaccgaaaag
     4681 gaaggtcaca aaaacgacgc catgatgcga agcggccttg ccgatgccag aagggaactg
     4741 atccggctcc tggagaagaa gtcccgggag aaaccggccg agtaaaaaca gggaaagcgg
     4801 tgtcttcttt caaaatcggg aggagcccgg gatgaaattc ttcaaaatca tactggcggg
     4861 attgttttgt ctctgcctgt tgccggcgcc ggccgccggc gcggaatcgc gcgatccgtc
     4921 catcgccgcc ggccaggcgg ccctgaaaca ggggatggcc gcggccggca aaaagaactg
     4981 gccgctggcc gtcaagcact acaccgaggc ccagcggcgc ctccacctct cgccccaggt
     5041 catgtacaac ctggccctgg cccacgccca ggccggcaac gaattgctgg ccgacgtctg
     5101 gttccgggcc tacctgggag cggtgccgca ggcggccaac gccgaccagg tccgaaccga
     5161 gatctcccgg ctggaacagg cggccgatga aaaagtccag cggctcttca agcaagccga
     5221 ggatctggcc gaagcgctcc cgctggaggc caacaatcag ggccgcggcc ggcgggcgga
     5281 ggccttcaaa tcgatcgcca atatcaggat cgccgtggga gacttcaagc gagccgaaga
     5341 agacctgctc aaaatgaatg cctacttcag cggaaagaac gtccccgata ggtactaccc
     5401 cgatgcaaac accaggtatg ccgacttcgc cggaaccctg gccgaggcgg gcgacgtcga
     5461 aggagcccgg cggctgcgcg gccagctgaa aaacggcgac ggtctggcga tgccgattct
     5521 gatggccctg gtcgaggcgg gggatctgaa ggaagcgaaa gaatttcttg aagatgaaaa
     5581 agaaacccgg tttggtgtcc ggcttgaacc ctatgaaacg gcgctgcgag ctttcgccga
     5641 aaaaggggat cttgcgaccg ctgaaaaaat catcggagcc gcccgaaatt attctcaaga
     5701 ttatcccgaa tacctccacc tggaattagc ccacctgctg cgcgaccagg gcgacctgac
     5761 cggggctcaa cggtatgccc gcctaaccga taattatcgt acggccatcg ccataaaaag
     5821 cgacatcgaa gaatcgctcc ggggcctgga aaattccggg gtgccggttt ttcagcctta
     5881 ctatgccgtg gagaccatcg ccgacatcgt caaaacctgc ctctggctgg gggaaacgcg
     5941 gaaggccgaa aaggcgctca aaatcggcga cgcctggata gcaaaaaaca ggaggcaact
     6001 cggaggactc ggaggagatg ggaaatccgt cgccgaaggg tatctccgaa tcggccgcgc
     6061 ctacctggag gtggaaaacg gccggggcgc cgaggcgata aaactcgttc agtccatctc
     6121 ctttccctgg gagatttcgg aagcggccgg agagtttata cagcaggcct atcagattga
     6181 attgaaacgc gacctggcca atttcgcgat ttcccgcggg aaaccggcgg cggccgaaag
     6241 gatcgccgat tccttcgccg agagccggga gcggatcgag ttcttaaggc gggtgctggc
     6301 gctttacgat tcgaaggccg accgcgccaa tacggagcgc ctgaccaaaa aactggccgg
     6361 cctgctggcc gaggtgcagg ccggcggctg ggatccggaa aaccccggcc gcgagttcgt
     6421 ggtcaacgcc tggacggacg cggcctatgc gctggtctgg ggagttcatc cccgaacatt
     6481 tcggacctcc tcaatgaaaa tactcgagtg gagaggggct ccggcgatct ataacctgcc
     6541 ggcctatatc gctgaaatca aaaaaacgag ttccccggat gcgatgcccg cctcatttgc
     6601 cgaaagcgcg aaaaccatcg gcaagggact tctgcggatc cgggcgctgg accggatcta
     6661 ccggtaagac g
//
LOCUS       DAOQYN010000103         6645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_11911_length_6645_cov_3.932777, whole genome shotgun sequence.
ACCESSION   DAOQYN010000103 DAOQYN010000000
VERSION     DAOQYN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11911_length_6645_cov_3.932777"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PKN80_05195"
     CDS             <1..438
                     /locus_tag="PKN80_05195"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282986.1"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNQ35445.1"
                     /translation="TAGEPSGRLVVRGGHPLRGVTISGPEIPNLIDELPLAAVLGLNA
                     RGRTEVRDAAELRVKESDRIAAIVGELRRLGAEITERPDGFIIEGPQRLRGGRADSRG
                     DHRIAMCLAVAGLAAAGETVIEDSGCVAVSFPGFEAALGSIIA"
     gene            496..1665
                     /locus_tag="PKN80_05200"
     CDS             496..1665
                     /locus_tag="PKN80_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ35446.1"
                     /translation="MKPTPSFSENRKEETGRRVALAGCADYRPETVAAALERLAAALD
                     LPERFRSGERVLVKPNFLFPAGDDRPLTTHSEVILVCCRMLLDLGCRVALGDSPGWGS
                     LAAVLERKGLVGPLKQLGVSVAEFSAVGEWPLPEGRIVKRLPLAAVLDDFDAVISLAK
                     LKTHGQTYFTGSVKNLYGCLPGTIKPEYHLRFRESGQFARLLLDVHDAVRPRLGIMDG
                     VIGMEGEGPSSGRPKAAGFLAASADCAALDIAAMREVGLAPERVEHLRERLAGPVFEV
                     VRLGKIEAPSFEPPPGLKPLPAVIPLPGFLRRALRRLVVPFPVFAPPPVCRLCGECVR
                     ICPVRPRAVELAGGRPVLHRARCIQCYCCHEMCPARAISLSRLPLRRIWLGLKKG"
     gene            complement(1682..2416)
                     /locus_tag="PKN80_05205"
     CDS             complement(1682..2416)
                     /locus_tag="PKN80_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNQ35447.1"
                     /translation="MIEISAAGWITAGLAALMFGISKTGLPGLGMLAIPLMADILGAK
                     TSTGIVLPMLIFADIFAVAYYHRQAVWKHLLRLAPWAAAGVVLGYRLMGRIDDRQLKP
                     LIGLIVLAMLAVHLWRSRRPDAPIPTHWLFSAVIGILAGVTTMLANAASSILAVYFLS
                     LRLPKNEFVGTGAWYYMILNWFKVPFSVSLGLINPDSLRFNLMLFPLVGAGALAGILL
                     LRVIPERPFNRIVQLLTAAAAVKLLF"
     gene            2539..3273
                     /locus_tag="PKN80_05210"
     CDS             2539..3273
                     /locus_tag="PKN80_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNQ35448.1"
                     /translation="MDRSLVLLADIHANLEALHAVLRATEAEAADYFCLGDAVGYGAN
                     PAECLKLLRDSGVPLVAGNHDRAVRHPGLTSIFTPEARQAVFWTIEHLFPADRDQLDG
                     LPGTVARDGLRFVHAGPLEPETWPYLVEEAELTANLRALAPGEICFFGHTHRPGLYEW
                     RAGRFAARSLEGTVRLDPGASYLVNPGSVGQPRDGDPRAAYLVFDPAARTVRLERVSY
                     DVAAAQEKILRAGLPEALARRLAFGY"
     gene            3295..4008
                     /locus_tag="PKN80_05215"
     CDS             3295..4008
                     /locus_tag="PKN80_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35449.1"
                     /translation="MGGGFFQGIILAGLLLVMGIQGWYLVSLKLEGEIGWWRFAAVLV
                     SFAPGILVAALSLPGGGGETISSLPWPVNLAALFLPLICLGIFRSLENRYGLRREAEW
                     CLREIGVWKATLLKDPGHVGALVMLGDLYLRLGEREKAAEYYHQALELKPQDPAIQKK
                     IEFLEKKMELVPAFTREDLGVVGKELKRLPLYLLGATGAVLLIILFVFLLSVLPPAAV
                     FVLVVLVPIILLGVWLLRS"
     gene            complement(4019..5287)
                     /locus_tag="PKN80_05220"
     CDS             complement(4019..5287)
                     /locus_tag="PKN80_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HNQ35450.1"
                     /translation="MRTIIFEDDGFQALYPLTYLRPVFGLRCGATTLYEKIRRHFGAG
                     LPAAALRPGLAALWRREHPGAGTIETDAPFQAGPEPMLLVNGRWLAEPPGIPATGPEE
                     AAFAGGDLAYLRLRPETAAALPAQTWNGFLAAAVEKLPRREVELKLIRHPWDLINHNP
                     DAIRTDFQLAGKSGLEGEFHPQSVIFGDPGRVYLAAGARVHPGVVLDTEHGPVTIDAG
                     AEIFPFSRLEGPCYIGPGTLITGSAKIREGTSIGPVCRVGGEVEESIIQGYSNKYHDG
                     FLGHAFVGEWVNLGAFTTNSDLKNDYGSVTVYLQGKPVDTGSTKVGSFIGDHTKTGIG
                     TLLNTGTVLGVMCNVVGSGSLPPKYVPSFCWFIEGKFLKYSLNAQLETARLAMSRRKV
                     ELSPEMETLIRQTHELTREERLKAVRRTAG"
     gene            complement(5284..6528)
                     /locus_tag="PKN80_05225"
     CDS             complement(5284..6528)
                     /locus_tag="PKN80_05225"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020005715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNQ35451.1"
                     /translation="MKVLVINSGSSSIKYQLFEMPRSEVIARGLLEKIGEEISPFRQK
                     SGRGEVEIKTRVPDHEAGLNLIIRSITDPETGVVASRNEIAAVGHRVVHGGEEFTGSV
                     LITPRVMAKIRKYADLAPLHNPPNLMGIRAARKFLPGAVQTASFDTAFHTTIPDKAAL
                     YAIPHHFYHRYRIKRYGFHGTSHRYVARRLAEILKRDKTELNLITCHLGNGCSITAIE
                     QGRSVDTSMGLTPLEGLVMGTRSGDLDPAIIFYLADKGYNPKEINNILNKQSGLLGLS
                     GQSNDMRNLVALREGGDPRAALAIDIFCYRLRKYLGAYLAVLGRTDAIIFTGGIGENN
                     PLIRTLTLQGLEGLGIRLDEKRNRLTRAVEGEISAPGAPIRVYIIPTNEELRIAGDAY
                     EIASRRPGNAIGPAASKKRRRK"
     gene            complement(6525..>6645)
                     /locus_tag="PKN80_05230"
     CDS             complement(6525..>6645)
                     /locus_tag="PKN80_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNQ35452.1"
                     /translation="GPLLQGCARPVSDLSRGATSADIVGIAAIVAVQAQEPAG"
BASE COUNT          957 a         2153 c         2357 g         1178 t
ORIGIN      
        1 accgccggcg aaccctccgg ccgcctggtg gtcaggggcg gccatcccct gcgcggggtg
       61 acgatttccg gcccggagat tccgaacctg atcgacgaac tgccgctggc ggcggttctc
      121 gggcttaacg cccggggccg caccgaggtc cgggacgccg ccgaactgcg ggtcaaggag
      181 agcgatcgga tcgcggccat cgtgggcgag ttgcgccgcc tgggggccga gataaccgag
      241 cgaccggacg gcttcatcat cgagggtccc caacgtctca ggggtggccg ggccgattcc
      301 cggggcgacc accggatcgc gatgtgcctg gcggtggccg gcctggcggc cgccggcgaa
      361 accgtcatag aagactccgg gtgcgtggcc gtctccttcc ccggctttga agcggcgctc
      421 ggttcgatca tcgcctgagg ccgggcggct ccggccggcc gcgccgctcc cttgccttgc
      481 ccctggatgc gaatcatgaa acccacgcct tccttttccg aaaaccggaa agaagagacc
      541 ggccgccggg tggcgctggc cggctgcgcc gattaccggc cggagacggt ggccgcggcc
      601 ctggagcggc tggccgcggc cctcgacctg cccgagcgat tccggtccgg cgagcgggtc
      661 ctggtcaagc ccaatttcct ctttcccgcc ggggacgacc ggccgctgac cacccactcg
      721 gaagtcatcc tggtctgctg ccgaatgctg ctggatctgg gctgtcgggt cgccctgggc
      781 gacagcccgg gctggggcag cctggccgcc gtcctggagc gcaagggcct cgtcgggccc
      841 ctgaagcagc tgggcgtgtc ggtggccgag ttctcggcgg tcggcgagtg gccgctgccg
      901 gaaggccgga tcgtcaaacg gctgccgctg gccgcggtcc tggatgattt cgacgccgtc
      961 atctcgctgg ccaagctgaa gacccacggc cagacctact tcaccggcag cgtcaagaac
     1021 ctttacgggt gcctgcccgg gaccatcaag cccgagtacc accttcgttt ccgggagtcc
     1081 ggccagttcg cccggctgct gctggacgtg cacgatgcgg tccggccgcg cctgggcatc
     1141 atggacgggg tgatcggcat ggaaggggag gggccgtcca gcggccggcc gaaggcggcc
     1201 ggtttcctgg ccgcctcggc cgactgcgcc gccctggata tcgcggcgat gcgggaggtc
     1261 ggcctggccc cggaacgggt cgagcacctg cgggaacggc tggccggtcc ggttttcgag
     1321 gtggtacgcc tggggaaaat cgaggcgccg tctttcgagc cgccgccggg tttgaagccg
     1381 ctgcccgcgg ttatcccgct gcccggtttc ctgcggcggg ccttgcggcg gctggtggtc
     1441 ccctttccgg tcttcgcccc gcccccggtc tgccgtctct gcggggagtg cgtccggatc
     1501 tgcccggtcc ggcccagggc ggtcgagctc gccggcggcc gcccggtcct gcaccgcgcc
     1561 cgctgcatcc agtgttactg ctgccacgaa atgtgcccgg cccgggccat cagcctgtcg
     1621 cgcctgccgc tgcgccggat ctggctcggg ctcaagaaag gttgagaggc cggggaaaag
     1681 gtcagaagag aagcttgacg gccgcggcgg ccgtgagcag ctggacgatt cggttgaagg
     1741 gccgctccgg gatgacccgc agcagcagga ttcccgccag cgcgcccgcg ccgaccaggg
     1801 gaaagagcat caggttgaaa cgcagcgaat ccgggttgat cagccccagg ctgacgctga
     1861 acgggacctt gaaccagttg aggatcatgt agtaccaggc gccggttccc acgaactcgt
     1921 tcttgggcag gcgcagggag aggaagtaga cggccaggat cgagctggcg gcgttggcca
     1981 gcatggtggt gaccccggcc aggatgccga tgaccgcgct gaagagccag tgggtcggta
     2041 tcggggcgtc gggccgccgg ctgcgccaga gatggacggc cagcatggcc agtacgatca
     2101 ggccgatcag cggcttgagc tggcggtcat cgattcgccc catcagccgg tagccgagca
     2161 ccacgccggc cgccgcccag ggggccaggc ggagcaggtg cttccagacc gcctggcggt
     2221 ggtagtaggc gaccgcgaag atgtcggcga agatgagcat cggcagcacg atgccggtgg
     2281 aggtcttggc cccgaggatg tcggccatca gcgggatggc cagcatgccc aggccgggca
     2341 ggccggtctt ggagattccg aacatcaggg cggccagccc ggccgttatc cagccggcgg
     2401 cggagatctc gatcatgttt gtctcccggt gcgggatgcc atataatgga agcaataatt
     2461 ctaacaccgt tgtcccggcc ggtcaaaccc cggattttga ttattttaat aacgattata
     2521 attataattt aagaaaccgt ggaccgttcc ctggtgctcc tggccgacat ccacgccaac
     2581 ctcgaggcgc tgcacgcggt gttgcgcgcc accgaagcgg aggcggccga ttatttctgc
     2641 ctgggcgacg cggtcgggta cggggccaat ccggccgagt gcctgaaatt gctgcgcgat
     2701 tcgggcgtgc cgctggtggc cggcaaccat gaccgggcgg tacggcaccc gggcctgact
     2761 tcgatcttca ccccggaggc gcgccaggcc gtcttctgga cgatcgagca cctctttccg
     2821 gccgaccggg atcagctgga cgggcttccc ggcaccgtcg cccgggatgg cctccgtttc
     2881 gttcacgccg ggccgctgga gccggagacc tggccctacc tggttgagga agcggagctg
     2941 accgcgaacc tgcgggcgct ggcgccgggc gaaatctgct tctttggcca tacccaccgg
     3001 cccggcctct acgagtggcg ggccggccgg tttgccgccc gttccctgga gggaacggtc
     3061 cggctggacc cgggggccag ctacctggtc aatccgggca gcgtcggtca gccgcgcgac
     3121 ggcgatcccc gggccgctta cctggtcttc gatccggcgg cgcgcacggt gcgcctcgag
     3181 cgggtcagtt atgacgtggc cgccgcccag gagaagatcc tccgggccgg gctgccggaa
     3241 gcgctggccc ggcggctggc gtttggttat tagcgggcgg ggaggggagg gcaaatgggc
     3301 ggcggtttct tccagggcat cattctggcg ggcctgctgc tggtcatggg gatccagggc
     3361 tggtacctgg tcagcctgaa actcgagggc gagatcggct ggtggcgttt cgcggccgta
     3421 ctcgtctcct tcgcgccggg catactggtg gcggctctca gcctgccggg cggcggcggg
     3481 gagaccattt cttccctgcc ctggccggtc aacctggccg cgctcttcct gccgctgatc
     3541 tgcctgggaa tattccggtc gctggagaat cgttacggtc tgcggcgcga ggcggaatgg
     3601 tgcctgaggg agatcggggt ctggaaggcc accctgctca aggatcccgg ccacgtcggc
     3661 gccctggtga tgctgggcga cctctatctc cgtctcggcg agcgggagaa ggcggccgag
     3721 tattaccacc aggccctcga attaaaaccg caggacccgg ccattcagaa gaagatcgaa
     3781 ttcctggaga agaagatgga gctggtgccc gccttcaccc gggaagacct gggcgtggtc
     3841 gggaaggaac tgaagcgcct gccgctttac ctgctgggcg caaccggggc cgtcctgctg
     3901 atcattcttt tcgtcttcct gctcagcgtc ctgccgccgg cagccgtctt cgtcctggtc
     3961 gtcctggttc cgatcatcct gctcggcgtc tggctgctcc gctcctgacc cggccgattc
     4021 atccggccgt ccgccgcacc gccttcagcc tctcctcgcg ggtcagttcg tgcgtctgcc
     4081 ggatgagcgt ctccatctcc gggctcaatt cgaccttccg gcgcgacatg gccaggcgcg
     4141 cggtctccag ctgggcgttc aggctgtact tcaggaactt gccctcgatg aaccagcaga
     4201 aggaggggac atatttgggc ggcaggctgc cgctgccgac cacgttgcac attaccccca
     4261 gcacggtgcc ggtgttgagc agggtgccga tgccggtctt ggtgtggtcg ccgatgaagg
     4321 agccgacctt ggtgctgccg gtgtcgaccg gcttgccctg caggtaaacg gttacgctgc
     4381 cgtagtcgtt cttcaggtca ctgttggtcg tgaaggcgcc caggttgacc cattccccca
     4441 cgaaggcgtg gccgaggaag ccgtcatgat acttgttgga gtatccctgg atgatgctct
     4501 cctcgacctc gccgcccacc cggcagaccg ggccgatcga ggtgccttcc cggatcttgg
     4561 ccgacccggt gatcagggtg ccgggcccga tgtagcaggg accctccagg cgcgaaaacg
     4621 gaaagatttc ggccccggcg tcgatggtga ccgggccgtg ttcggtatcc aggaccacgc
     4681 ccgggtgcac ccgggccccg gcggccaggt agacccggcc gggatccccg aagatgaccg
     4741 actgggggtg gaactcgcct tcgaggccgc tctttcccgc gagctggaaa tcggtccgga
     4801 tggcgtcggg attgtggttg atcaggtccc aggggtggcg gatcagcttg agctcgacct
     4861 cccggcgcgg gagtttttcg acggccgccg ccaggaagcc gttccaggtc tgggccggaa
     4921 gcgcggcggc cgtctccggc cgcagccgga gataagccag gtcgccgccg gcgaaggcgg
     4981 cctcctccgg gccggttgcc gggatgccgg gcggttccgc cagccagcgg ccgttgacca
     5041 ggagcatcgg ctcggggccg gcctggaagg gagcgtcggt ttcgatggtc ccggcgccgg
     5101 gatgctcccg gcgccagagg gcggccagcc cgggccggag cgccgcggcc gggagcccgg
     5161 ctccgaagtg gcgcctgatc ttctcgtaga gcgtggtcgc cccgcagcgc aggccgaaga
     5221 ccggccgcag gtaggtcagc gggtaaaggg cctggaaacc gtcatcctcg aagatgatgg
     5281 tcctcatttc cgtctccttt tctttgaggc ggccggtccg atggcgtttc ccggccgccg
     5341 gctggcgatt tcgtaggcgt ctccggcgat gcgcagctct tcgttggtcg ggatgatgta
     5401 gacccggatc ggcgcgcccg gcgcgctgat ctccccctcg accgcccggg tcagccggtt
     5461 gcgcttttcg tccagccgga tgcccagacc ctcgagtccc tgcagggtga gggtccggat
     5521 gagcgggttg ttttcgccga tgccgccggt gaagatgatc gcgtcggtcc ggcccagcac
     5581 ggccaggtag gcgcccaggt acttgcgcag ccggtagcag aagatgtcga tggccagggc
     5641 ggcccgcgga tcgccccctt cccgcagggc gaccaggttg cgcatgtcgt tgctctggcc
     5701 cgacagcccg agcaggccgc tctgcttgtt caggatgtta ttgatttcct tggggttgta
     5761 acccttgtcg gccaggtaaa agatgatggc cgggtccagg tcgccggaac gggttcccat
     5821 gaccagcccc tccaggggcg tcaggcccat gctggtgtcg accgaccggc cctgttcgat
     5881 ggccgtgatg ctgcagccgt tgcccaggtg gcaggtgatc aggttcagtt ccgttttgtc
     5941 ccttttcagg atttcggcca ggcgcctggc cacgtagcgg tgcgaggtgc cgtggaagcc
     6001 gtagcgtttg atccggtacc ggtggtagaa atggtggggg atggcgtaaa gggccgcctt
     6061 gtcgggtatg gtggtgtgaa aggcggtgtc gaagctggcg gtctgcacgg ccccgggcag
     6121 gaacttgcgg gcggcccgga tgcccatcag gttgggcggg ttgtgcaggg gggccaggtc
     6181 ggcgtacttg cggatcttgg ccattacccg cggggtgatc aggaccgagc cggtgaactc
     6241 ctcgcctccg tgtaccaccc ggtggccgac ggcggctatt tcgttccggg aggccaccac
     6301 cccggtctcc ggatcggtga tgcttcggat gatcaggttg aggccggctt cgtggtccgg
     6361 gacccgcgtt ttgatctcaa cttcgccgcg gccgctcttc tgccggaagg gagaaatctc
     6421 ctccccgatc ttctcgagca ggccccgggc gatcacctcg gaccggggca tctcgaagag
     6481 ctggtacttg atcgaggaac tcccggagtt gataaccagt accttcatcc ggccggctcc
     6541 tgcgcctgga ccgccacgat ggcggcaatg cccacgatgt cggcgctggt cgctccccgg
     6601 gagaggtcgc tcaccggccg cgcgcagccc tgcaggagcg gcccg
//
LOCUS       DAOQYN010000104         6559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_12111_length_6559_cov_4.585025, whole genome shotgun sequence.
ACCESSION   DAOQYN010000104 DAOQYN010000000
VERSION     DAOQYN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12111_length_6559_cov_4.585025"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2499)
                     /locus_tag="PKN80_05235"
     CDS             complement(<1..2499)
                     /locus_tag="PKN80_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35453.1"
                     /translation="MKKKLASKKNRTRKVLPALVAGILLTGSGVMGKTAEAQTAPTTA
                     RIRAWQEYPFVKPPAAGVHRQLWEDFTDLPEWCQRGRIVYGAEQTSIEAAKRIKENGA
                     GLLHVGPIRALSAEQVKTYEEIGLPLVLRLDGQMFWGDRHNELMETLRPGGWAVYHST
                     VPNNDWFRKFPASLSAAKFMRDGQPMWEYYGSNWVMRRDINYIHPAAIQMRDAFLREC
                     LLGEQVFDRPIGLAAAGHASGVWWDNPGNIVESYDAYTQGFVANEVKRLFEKRFRQRF
                     EAEFNQQPALQKRYPTPEAKENLFYQRYQAWYVDPLYFWMRDGDEEVLEWWNRFWAEA
                     YAGYYAWQYQYIQNEIAPKLGKKHLFVGGNSKLCWSSASWDYYAFSWGTYDLLGPNET
                     TVVYSDKHAPGFKLALAASNGKPAALWNGGNNDLVCAEALACLGVVGNQPEHLTAFQK
                     ANFDLYQNARPGGRVACLYHLEDGLHHSEIANLCRVTDQVGRSGVPLEIITEKHLGPE
                     VLDTFDLVILPGFRFTDAEAAALRAYAERGGRLLLLGDNRDRNGRPLAEGLAGLAAWT
                     DGEKALGKGQVRVFGSQLIDQAAMDGTLAALKADAFRITSPAGANLLLNILTQPKQNL
                     VTAHLVNFTGQPVENVRVRFPDDLNLTRIVYISPYGSSQTLKAVGKTVTIPRLSIYGI
                     ICLCPNERIARDLLERNRGFNRTFENDRLPRVTVRGGIPEKEWKLSELAPGEKLCQLR
                     QFTKTGFRRLDADVVTAGRGRVGTAQPVKLRIHALGVWNPEAVFLDRMEFVFVHEASG
                     HREIVPLKMEPVSGQEKESGADERQKSQVQMPAGT"
     gene            complement(2518..3522)
                     /locus_tag="PKN80_05240"
     CDS             complement(2518..3522)
                     /locus_tag="PKN80_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35454.1"
                     /translation="MLAKQPPLARWTWLRNPANPILKPDPESLYDCRSCMNPFVIRVE
                     DQYRVYYSGGDAGGRQRICLATAPVDRPTEFNRRGVVLDLGRKGDFDGKWCVTPLVRR
                     FGDRWHLYYTGNDGGGLGLQSFCGIGLAVSQDGLGFRRLSTDPVITGDRVREFPKNRG
                     VAGGGTITEERGPDGAPAYRLYYTLTVGTKNPDVRLDQEKHCAVAHSRDGLRWTDHRL
                     IMSPRPEVANEDIAVAGPVVWREENLYRMLYCGIGSRWGFYSISEAASFDGYAWHRGR
                     GDENLSLAPGPADSWENQMVEYPAVIEEGNLLRIYYCGNGYGKTGIGTATSVRTGKRG
                     "
     gene            complement(3516..4328)
                     /locus_tag="PKN80_05245"
     CDS             complement(3516..4328)
                     /locus_tag="PKN80_05245"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ35455.1"
                     /translation="MRRKNKLRGQTNPIPVTGLPPGVVRLDYRSRFDGQPDWALAWTP
                     PRGNDWLVCLHGHGSGGSQLYVRPDLREHWLPAFRKLGLGILTPNLRGNNWMGPAAAD
                     DLADLLERLRSEFGAGRFFFFSGSMGGTGNLIYAIRRPGDAAALVALAPAADLTSYHA
                     WCRRGNRAILREIADAIEKAYGGSPDGRPAAYRRHSALEHHEKLVMPTFIAHGREDAV
                     IPVEQSRRLAEKRGGDAGFVYREIEGGHDAPLLLPEAVEWLADLVSRKEKRC"
     gene            complement(4365..5666)
                     /locus_tag="PKN80_05250"
     CDS             complement(4365..5666)
                     /locus_tag="PKN80_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNQ35456.1"
                     /translation="MNEIAAGILGLLLALLLFFTGIELGFAMALVGFLGFCYLVSVPA
                     GLNLLAKDIFDVFASYSFTVIPLFVLMGQIAFNAGIAKRLYRTAYRFIGHIPGGLAMA
                     TVAGATAFKAICGSSPATAATFASVAVPEMDRYHYDRKLSTGIVATVGTLGILIPPSV
                     TLIVFGVITEQSIGRLFLAGIIPGLLIALFFMLIIYGWCRINPTLGPRGERTAWKERL
                     ASLPEFLSVGAIFLLVIAGLMKGFFTPTEAGSVGTFAVLLLAILRRDLSWPNLVKSVS
                     ESLRTAGMVLMLIAGSTILGHFLAVTRIPFVAADWVAGLPLHRYTVMVIIALIYLVGG
                     SFIDDLAFMVLATPIFYPVIVKLGFDPIWFGMIIGITVMIGVVIPPVAINVFVVRNIT
                     KVPFNVIYRGIYPFLISLVFCALLLFLFPALATFLPNLLMK"
     gene            complement(5659..6144)
                     /locus_tag="PKN80_05255"
     CDS             complement(5659..6144)
                     /locus_tag="PKN80_05255"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNQ35457.1"
                     /translation="MFTTFLKRIRGTCSLLNLIAGISLVFLMLLTMADVILRAFKHPI
                     PGTYELVGLAGAVAIGFSMPITSWFRGHIYVDFFTSRFPRRVRNGFNIVTRLLAIGLF
                     LLVGWNLFRFGLDLKNAGEVTLTLQLPFYPVVYGVGIAFFVQCLVLIGDLVKIFRGEY
                     E"
     gene            complement(6215..>6559)
                     /locus_tag="PKN80_05260"
     CDS             complement(6215..>6559)
                     /locus_tag="PKN80_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNQ35458.1"
                     /translation="TTESYGAAYTTGFFVVMNLNRWNSLPPDVRKIIEKVNDEWIDKT
                     GRGWDEIDRAGRDFTLQRKNQFIRLSETENRRWAAAVQPILKEYAESMKSKGLPGELA
                     LKFCLEQLKKSR"
BASE COUNT         1123 a         2044 c         2160 g         1232 t
ORIGIN      
        1 ggtgccggcc ggcatctgga cctggctctt ctgccgttcg tcggcgccgc tctctttctc
       61 ctgcccgctt accggttcca ttttcaacgg tacaatttcc cggtggccgc tcgcctcgtg
      121 gacgaagacg aattccatcc ggtccaggaa gaccgcctcc gggttccaga cgcccagggc
      181 gtggattctc aatttgaccg gctgggcggt gccgacccgg ccccggccgg cggtcaccac
      241 gtcggcatcc aggcgccgga agccggtctt ggtgaattgg cgcagctggc agagcttttc
      301 acccggcgcc agttccgaga gtttccattc cttctccggg ataccgccgc gcaccgtcac
      361 gcggggcagc cggtcatttt cgaaggtccg gttgaagccg cgattccgtt ccagcagatc
      421 ccgggcgatc cgttcgttcg ggcagaggca gatgatgccg tagatcgaga ggcgcggtat
      481 ggtgacggtc ttcccgacgg ccttgagtgt ctggctcgat ccgtagggcg aaatgtaaac
      541 gatccgcgtt aaattcagat cgtccgggaa gcggacccgg acgttttcga ccggctggcc
      601 ggtgaagttg accaggtggg cggtcaccag gttttgcttc ggctgggtca ggatgttgag
      661 caggaggttg gccccggcgg gactggtgat ccggaaggcg tccgctttaa gcgcggccag
      721 ggtgccgtcc atggccgcct ggtcgatcag ctggcttcca aagacgcgga cctgtccttt
      781 gcccagcgcc ttctcgccgt cggtccaggc cgccaggccg gccaggccct cggccagcgg
      841 ccggccgttc cggtcgcggt tgtcacccag gagcagcagg cggccgcccc tctcggcgta
      901 ggcgcgcaag gcggcagcct ccgcgtcggt gaacctgaag cccggcagaa tcaccaggtc
      961 gaaggtgtcc aggacttccg gtcccaggtg cttttcggtg atgatttcca gcggcacgcc
     1021 gctccgcccg acctggtcgg tcacccggca gaggttggcg atctcgctgt gatgcaggcc
     1081 gtcttccagg tggtagaggc aggccacccg gccgccgggc cgagcattct ggtacaggtc
     1141 gaagttggcc ttctgaaagg cggtcaggtg ctccggctgg tttccgacca cgcccaggca
     1201 ggcgagggcc tcggcgcaga ccaggtcgtt gttgccgccg ttccagaggg cggccggttt
     1261 gccgttggag gcggccaggg ccagtttgaa accgggcgcg tgcttgtcgg aatagaccac
     1321 cgtggtttcg ttcggcccga gcaggtcgta ggtgccccag gagaaggcgt agtagtccca
     1381 ggaggccgaa gaccagcaga gcttgctgtt gccgcccacg aagaggtgct tcttgcccag
     1441 cttgggcgcg atttcgttct ggatgtactg gtactgccag gcatagtagc cggcgtaggc
     1501 ctcggcccag aagcggttcc accactccag cacctcctcg tctccgtccc gcatccagaa
     1561 atagagcgga tccacgtacc aggcctggta acgctggtag aagagattct ccttcgcctc
     1621 cggggtcggg taccgtttct gaagggccgg ctgctggttg aactcggctt caaacctttg
     1681 gcggaagcgt ttttcgaaga ggcgcttgac ttcgttcgcc acgaaaccct gggtgtaggc
     1741 gtcgtaactc tcgacgatgt tgcccgggtt gtcccaccag acgccggagg cgtggccggc
     1801 ggcggccagt ccgatcggcc ggtcgaagac ctgttcgccc agcaggcatt cgcgcaggaa
     1861 ggcgtcgcgc atctggatgg ccgccgggtg tatgtagttg atgtcgcgcc gcatcaccca
     1921 gttgctgccg tagtattccc acatcggctg tccgtcgcgc atgaacttgg cggcgctgag
     1981 actggccggg aacttccgga accagtcgtt gttgggcacc gtgctgtggt aaacggccca
     2041 gccgccgggc cggagggtct ccatcaattc gttatggcgg tcaccccaga acatctgccc
     2101 gtccagccgc agcaccaggg gcaggccgat ctcctcgtag gtcttgacct gctccgcgct
     2161 gagcgcccgg atcggcccga cgtggagcag gccggcgccg ttctccttga tccgtttcgc
     2221 cgcttcgatg ctggtctgtt cggcgccata gacgatccgg ccgcgctggc accattccgg
     2281 caggtcggtg aagtcctccc agagctgacg gtgcaccccg gccgccggcg gtttgacaaa
     2341 cggatactcc tgccaggcgc ggatccgggc cgtcgttggc gcggtctgcg cctcggccgt
     2401 cttccccatc accccgcttc cggtcagcag tatcccggcc accagagccg gcagcacctt
     2461 ccgggtccgg ttcttcttcg aggccagctt ttttttcatg cctgtctccc tgtcggttca
     2521 accgcgtttc ccggttcgga cggacgttgc ggtaccgatg ccggtctttc catacccgtt
     2581 gccgcaatag tagatcctta gcagattccc ctcttcgatc accgccgggt actcaaccat
     2641 ctgattctcc cagctgtcgg ccggccccgg cgccagggac aggttttcgt ccccccgtcc
     2701 ccggtgccag gcgtagccat cgaaactggc ggcttcggag atggaataaa atccccagcg
     2761 gcttccgatg ccgcagtaaa gcatccggta aagattttct tcgcgccaga cgaccggtcc
     2821 ggccaccgcg atgtcctcgt tggcaacctc cggccgcgga ctcatgatca gccggtggtc
     2881 ggtccagcgg agtccgtccc gactgtgggc caccgcgcag tgtttttcct ggtcaaggcg
     2941 cacgtccgga tttttggttc cgaccgtcag ggtgtagtaa agccgataag ccggcgcgcc
     3001 gtccgggccg cgttcctcgg tgatcgttcc gccgccggcc acgccccggt tcttcgggaa
     3061 ctcccggact cggtcgccgg tgatcaccgg gtcggttgag agtcggcgga aaccgagccc
     3121 gtcctggctt acggccagcc cgattccgca gaaagactgg agtcccaggc cgccgccgtc
     3181 gttaccggtg taatacaggt gccagcggtc gccgaaacgg cgtaccaggg gagtgacgca
     3241 ccacttgccg tcgaaatcac ccttccggcc aaggtccagc accacgcccc gccggttgaa
     3301 ctcggttgga cggtcaaccg gggcggttgc cagacagatg cgctggcggc caccggcgtc
     3361 accgccggaa tagtataccc ggtattgatc ctccacccgg attacgaaag gattcataca
     3421 gcttcggcag tcgtaaaggc tttccggatc cggtttcaga atcgggttgg cgggatttct
     3481 cagccaggtc cagcgggcca gcggcggctg ttttgctaac atcttttttc ctttcgggag
     3541 acaaggtctg cgagccattc gacggcctcc gggagcagga gcggcgcatc gtggccgcct
     3601 tcgatttcgc ggtagacaaa cccggcgtcg ccgccgcgtt tttcggccag gcggcgtgac
     3661 tgttccaccg gtatcaccgc atcttcccgg ccgtgggcga tgaaagtcgg catgaccagc
     3721 ttttcatggt gttccagcgc cgagtggcgg cggtaggccg ccggccggcc gtcgggactt
     3781 ccaccgtacg ccttttcaat ggcgtcggcg atctccctga gaatggcgcg gtttccccgg
     3841 cggcaccagg catgatagga ggtcaggtcg gccgccgggg ccagggcgac gagggcggcc
     3901 gcgtcgcccg gccgccggat ggcgtagatc aggttgccgg tgcctcccat cgatccgctg
     3961 aagaagaaga aacggccggc cccgaactcc gagcgcagcc gttcgagcag gtcggccaga
     4021 tcgtcggccg ccgccggtcc catccagttg tttccgcgca ggttgggcgt caggatgccc
     4081 aggcccagct tgcggaaggc cggcaaccag tgctcgcgca ggtccggtct gacatagagc
     4141 tggctgcccc cggagccgtg tccgtgcagg cagaccagcc agtcgtttcc ccggggcggc
     4201 gtccaggcca gggcccagtc gggttggccg tcaaagcggc tccggtaatc gagtcgcacg
     4261 acgccgggcg gcaggccggt caccggaatg ggatttgttt gcccgcggag tttgttcttt
     4321 ctgcgcaact gttttacttt cgccgacccg gccggtggcg gttcttattt catcagcagg
     4381 ttgggtaaaa aggtcgccag ggcggggaag agaaagagca gcagggcgca gaagaccagg
     4441 ctgatcagga atggatagat tccccggtaa atcacgttga aggggacctt ggtgatgttg
     4501 cggactacga agacgttgat ggccaccggc gggatcacca ccccgatcat gaccgtgatc
     4561 ccgatgatca tcccgaacca gatcgggtcg aagcccagct tgacgatgac cgggtagaat
     4621 atcggcgtgg ccagcaccat gaaggccagg tcgtcgatga aggagccccc caccaggtag
     4681 atcagggcga tgatgaccat caccgtgtac cggtgcagcg gcaggccggc cacccagtcg
     4741 gcggccacga acggaatccg ggtcaccgcc aggaagtggc ccaggatggt tgagccggcg
     4801 atcagcatca gcaccatgcc ggcggttcgc agcgattcgc tgaccgattt caccaggttc
     4861 ggccaggaga ggtcgcgtcg gagaatcgcc aggagcagca ccgcgaaggt tcccacgctg
     4921 ccggcctcgg tgggcgtgaa gaagcccttc atcaggccgg cgatgaccag caggaagatg
     4981 gctccgaccg acaggaattc cggcagcgag gccagccgtt ccttccaggc ggtccgttct
     5041 ccgcgtggtc ccagcgtcgg gttgatccgg caccagccgt agatgatcag catgaagaag
     5101 agggcgatga gcaggcccgg gatgatgccg gccaggaaga gacgtccgat ggactgctcg
     5161 gtgatgaccc cgaagacgat cagggtgacg ctgggtggaa taagtatccc cagggtgccg
     5221 accgtggcca cgatcccggt ggagagtttc cggtcgtaat ggtagcggtc catctcgggt
     5281 acggccacgc tggcgaaggt ggcggcggtg gccggcgagg agccgcagat ggccttgaag
     5341 gcggtcgcgc cggccacggt ggccatggcc agcccgcccg ggatgtggcc gatgaaccgg
     5401 taggcggtcc ggtagaggcg cttggcgatt ccggcgttga aggcgatctg tcccatcagg
     5461 acgaagagcg ggattaccgt gaagctgtag gaggcgaaga cgtcgaagat gtccttggcc
     5521 agcaggttca ggccggccgg caccgagacc aggtagcaga agcccaggaa tccgaccagg
     5581 gccatggcga agcccagctc gattccggtg aagaagagga gcagggccag caggagcccc
     5641 aggatgccgg cggcgatctc attcatattc accccggaag atcttgacca ggtcgccgat
     5701 cagcaccagg cactggacga aaaaggcgat tcccactccg tagactaccg ggtaaaaggg
     5761 aagctgaagg gtcagggtga cctcaccggc gtttttaagg tcgagaccga accggaagag
     5821 gttccagccg acgagcagga agagtccgat ggccagcagg cgggtgacga tattgaagcc
     5881 gttcctgacc cggcgcggga aacgcgaggt gaagaagtcg acatagatat gtccgcggaa
     5941 ccaggaggtt atcggcattg agaatccgat cgccaccgca ccggccagtc caaccagctc
     6001 gtaagttccc gggatcgggt gcttgaaggc ccgcaggatc acgtcggcca tcgtcagcag
     6061 catcaggaag accaggctga tcccggcgat caggttcagc agggagcagg ttccccggat
     6121 tctcttcagg aaggtggtga acattcctaa tcttatttgg cgtctctcag ggaagaagcg
     6181 ggggaggcgc gtccgcgcct cccccgtcgg tcggttaccg gctctttttg agttgttcca
     6241 ggcagaactt caaggccagt tcgcccggca gtcccttgga tttcatgctc tccgcgtatt
     6301 ctttcaggat cggctggacc gccgccgccc agcgccggtt ctcagtctcg ctcaggcgga
     6361 tgaactggtt ttttcgctgg agggtgaagt cccgtccggc ccggtcgatc tcatcccagc
     6421 cccggccggt cttgtcgatc cactcgtcgt tgaccttttc aatgatcttc cgcacgtcag
     6481 gcggcaggga gttccatctg ttgaggttca tgaccacgaa gaacccggtg gtgtaggcgg
     6541 cgccgtaact ctcggtggt
//
LOCUS       DAOQYN010000105         6534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_12175_length_6534_cov_5.457478, whole genome shotgun sequence.
ACCESSION   DAOQYN010000105 DAOQYN010000000
VERSION     DAOQYN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12175_length_6534_cov_5.457478"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2490)
                     /locus_tag="PKN80_05265"
     CDS             complement(<1..2490)
                     /locus_tag="PKN80_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35459.1"
                     /translation="MKRMAALLCLFSLALIAMPLASAAEPAGLSQFSPVEAQVNLVEN
                     GAYSRAVGGLPTGWTLEGSGRPYIFNRGAYHAAGIEVTDRRSPGAMLLSQPIRIERPE
                     AAYVLTGLLAGGAAETDEVVPTDSEKKGGGVLELRCYDASGKRLANAAEFSFQGARRG
                     MHLYRELNDGRIFQRCQLPAGTATVRVAWGLRQAIGRVNTDDLAFYRETGTGRPDQLA
                     VAAYDANLRSAWLKAGDKGAILVDGVRDPGPKADINPVLAGVWKYPFNNWLLQYAPKG
                     VIFDLGGPCAVDGIEVSMATSTSDVVDRQAGRFDPEIIRVAFSDDGINFGPEMELLYR
                     PELMQAEALMRVYYQGFRRAGRFVRLAGAESMAEVRFFGRAEPVSIGYRPLMVIRNGQ
                     VAYGVEVELDGLDDQSFMARVPKGNIHRIDRRRWVDVTLITPAGRLSESLVFDNFTPE
                     KIMRRQYFTPNPAALAGGADLELAYHDRVQVWDSPTTHSLQTDQAAPALVIKSALPAQ
                     PQLAAVNQELAALKKQLDRSRGEEKNRVAALAAAQLAPKLKNLGTEAKSIEEFFSQAG
                     SPRTLGPELLPSFEIKTPDGKFFYRVAVKAPDIFLISKYDSVTGRYISNAGHSGNYVT
                     AGEVAMANPKYLLQRRYLKDGESFEAAAAAADGWENVSVSMVLERGFRALFRISFELP
                     EAGDWTMCCKTLWQFPESKMILNGQAVGTPFRWGQQTALLRNCRAGANELLIQADASQ
                     ARINLETAPYQASYFHLYWLKTSQPVAAFGESQVFLPGLSGKATVVYAGGAPVVVDFP
                     RETRFQPAAARFADLLKPTSETGPITTRPSDG"
     gene            complement(2621..6229)
                     /locus_tag="PKN80_05270"
     CDS             complement(2621..6229)
                     /locus_tag="PKN80_05270"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNQ35460.1"
                     /translation="MRRWLGFLVAIGCLAAAAGPLTAQEKSISRMWTGAMLAGKSGWG
                     SYSGGGDSVPGAMSGMATDPAAGAKAVYRLPINPPLPAGTYKILIKEFHGGRVSFGLA
                     GTTSEPLTYARHVSAGWSGLVLTITAPAPELALTFQPTLKPEQSPTQNIILQGIYITT
                     DLNEIIGRDDIIRSGAGPVLAPGQVPPPPLRTDNYLENSSFELGIGHGWGKVWINQWD
                     DSTAADGRYSLRLPVIQMWYSTLGYYYRLNNQSGFESKFYRLPPGRYTLSAYLKADKA
                     CSVNLRLFGHPEKATQDGPGRTAGGGRADLTTEWKRFAFTFDVPEGPGNFWAVGLNME
                     WRTINLGFKVDSSLEEIRKADLPIPQNVWVDALQLERAETASPYRPRGGVEVGILCRQ
                     PGHILYENEPANLELLVYNPGKNTGTLEATWRIEDYFDRPVREETFRIPLRGETHLSR
                     KPAVPLDRRGIFRMLAWTREDAAGPDELSFSILPPNRHLNILYEPGRMGTDTGSDPNA
                     LAILKRANCNWVISKGIGRMEGVMPEPGKYRWSDEALQNVLDAKIMMLGQVFVNNAPA
                     YLKPYCGTNQGWEPEKKKRYLEIWGNYVYDLVSHYREKIRHWEVTNEPYFWCTPEDYA
                     DILRVASERIRQVDPGIKVIGFCSGNMPAYFPRAVRAADPKWYDGVSGHFYDTDPVKL
                     RYFASVLKKYDKSGWQTEAGPTFPSFYKTLPTLQQLYGEEHHANPSSQGVRDSNIRTL
                     QGELRCFAIGAIDHYFHYFSRFGNTAPTETTRRPGGGKEDVEYDGSLRPYAVGRSIAS
                     HLLEDARHYAEWRGDPRVTLGLFEKGGQTVGVVWSVDGRLLEIRPETAGGWQVYDWCG
                     NKLALGKDGSFQVDRFPRYFTVAAPPAQVAGMLDRGTVRGWFNVDARLVPAPTGTGFA
                     LNLLLHNEKESLQRVSIILGGRLGGFFANPAELQNLEIQGKENRRLILLLTGTPGGRV
                     LDLAVMARGTDFSIDFPFTFSLAAAQAAAGLDEVAGRGPDFSTVTIRTGPGNRMSPAD
                     YTPRFEAGYDAVNLYLAADITDDLLSPVPGNIPGERHDQVHFLIAAETAERRSSNNPR
                     ETLGRVASRSDCFVVLAPKAGGGAAAELRRGDGTVEKIESASFTATAGGYRLAVAVPW
                     EKLGMAAPKPGSSLVFNAVAFDADGVNPDWKTEWPWAGGPEFEYANPAGWGELLFR"
     gene            complement(6340..>6534)
                     /locus_tag="PKN80_05275"
     CDS             complement(6340..>6534)
                     /locus_tag="PKN80_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35461.1"
                     /translation="FFWVTEAKVFKAVPPAGFNFYDLMGNPIEPAGLRLTESPVYFTG
                     PADPAAAAAALKTMRLEAAN"
BASE COUNT         1033 a         2120 c         2187 g         1194 t
ORIGIN      
        1 gccgtcggaa ggccgggtgg tgatgggacc ggtctccgaa gtcggtttca gcaggtcggc
       61 gaagcgggcc gcggccggct gaaaacgggt ctccctggga aaatcaacca ctactggtgc
      121 gccgccggca tatacgacgg tggcctttcc ggagaggccg ggcaggaata cctggctttc
      181 accgaaggcc gccaccggct ggctggtttt gagccagtag agatggaagt agctggcctg
      241 gtatggggcg gtttccaggt tgatccgggc ctggctggca tcggcctgga taagcagttc
      301 attggcgccg gcccggcagt tccggagcag cgcggtctgc tgcccccacc ggaagggtgt
      361 ccccaccgcc tgaccgttca gaatcatctt cgattcgggg aactgccaga gggtcttgca
      421 gcacatcgtc cagtccccgg cctccgggag ctcgaaactg atccggaaga gggcccggaa
      481 gccccgctcc agcaccatgc ttacgctcac gttctcccag ccgtcggcgg cggccgccgc
      541 cgcttcaaaa ctctcgccat ccttcaggta ccgccgctgc aggaggtact tgggattggc
      601 catcgccact tcgccggcgg tcacgtaatt gccgctgtgg ccggcgttgg agatatagcg
      661 gccggtgacc gagtcgtact tggagatcag gaagatgtcc ggcgccttga ccgcgacccg
      721 gtagaagaac ttgccgtccg gagtcttgat ttcaaagctg ggtaggagtt ccggacccag
      781 cgtccggggg ctgcccgcct ggctgaagaa ctcttcgatg ctctttgcct cggtcccgag
      841 attcttcagc ttgggggcca gctgggcggc ggccagggcc gcgacccggt tcttctcctc
      901 gccccggctg cggtccagct gtttcttcag ggcggccagc tcctggttga cggcggccag
      961 ctgcggctgg gccggcaggg ccgatttaat aaccagcgcc ggggccgcct ggtcggtctg
     1021 cagggaatgg gtggtcggcg aatcccagac ctggacccgg tcgtggtagg ccagttccag
     1081 atcggcgccg ccggccaggg cggccgggtt gggcgtgaag tactggcgcc gcatgatctt
     1141 ttccggcgtg aagttatcga agaccaggct ctcggaaagg cggccggccg gggtgatcag
     1201 ggtgacgtcc acccagcggc gccggtcaat ccggtgaatg ttgcccttgg gaaccctggc
     1261 catgaaggac tggtcgtcaa ggccgtccag ttccacctcg actccgtaag cgacctggcc
     1321 gttgcggatg accatcagcg gccggtaacc gatggagacc ggttcggccc ggccgaagaa
     1381 gcggacctcg gccatgctct ccgccccggc cagccggacg aagcggccgg cccttcgaaa
     1441 accttgataa taaaccctca tcaacgcttc ggcctgcatc agttccgggc ggtaaagcag
     1501 ttccatttcc ggaccgaaat tgatgccgtc gtcgctgaag gcaacccgga tgatctccgg
     1561 atcgaaacgg cccgcctgcc ggtcgaccac gtcgctggtg gaggtggcca tcgaaacctc
     1621 gatgccatcc acggcgcagg ggccgcccag gtcgaagatc actcccttgg gtgcgtattg
     1681 cagcagccag ttgttgaagg ggtacttcca gaccccggcc aggaccgggt tgatgtcggc
     1741 cttcgggccg ggatcgcgga caccgtcgac cagaatggcc cccttgtcgc ccgccttcag
     1801 ccaggcactg cgcaggttgg catcgtaggc ggccacggcc agctggtccg ggcggccggt
     1861 cccggtctcc cggtagaagg ccaggtcatc ggtgttgacc cgcccgatcg cctggcggag
     1921 tccccaggcc acccggaccg tggcggtgcc ggccggcagt tgacagcgct ggaagatccg
     1981 gccgtcgttg agctcccggt aaagatgcat gccgcggcgc gccccctgga agctgaactc
     2041 ggcggcgttg gccagccgtt tcccggacgc gtcgtaacag cggagttcca ggacgccgcc
     2101 gcccttcttt tcggaatcgg tgggcaccac ctcgtcggtc tcggccgccc cgccggccag
     2161 gagcccggtc agcacgtaag cggcctccgg acgctcgatg cggatcggct gggaaagaag
     2221 catggcgccg ggtgaccggc ggtcggtcac ctcgatgccg gcggcatggt aggccccccg
     2281 gttgaagatg tatgggcggc cgctgccttc cagcgtccag ccggtcggca gtccgcccac
     2341 cgcccggctg taggcgccgt tctccaccag gttgacctgc gcctcgaccg gactgaactg
     2401 gctcaggccg gccggttccg cggctgacgc caggggcatc gcgatcaagg ccaggctgaa
     2461 caggcataac aaggctgcca ttctcttcat ggttcttcct ccctccattc tttggccggg
     2521 atctcccggc gcttgcccga gatacgggat ggttggtaac tccagctgaa ggcccggccg
     2581 ggcttccggc cgggcctgtt cttacaggta cggtttccgg ctaccggaag agcagctccc
     2641 cccagccggc cggattggca tattcgaact ccggcccgcc cgcccagggc cactcggtct
     2701 tccaatccgg gttgacgccg tcggcgtcga aggcgaccgc gttgaagacc aggctgctgc
     2761 ccggcttcgg cgccgccatc cccaattttt cccaggggac cgccaccgcc aggcggtagc
     2821 cgccggccgt ggcggtgaag gaggcggact cgatcttttc gaccgtgccg tcacctcggc
     2881 gcagttccgc cgccgccccg ccgccggcct tcggcgccag taccacgaaa cagtcggagc
     2941 gcgaggccac gcgccccagc gtctccctcg ggttgttcga gctccgccgt tcggccgttt
     3001 cggccgcgat cagaaagtgg acctggtcgt ggcgttcgcc cggaatgttt ccgggaaccg
     3061 gcgaaagcag gtcatcggtg atatcagccg ccaggtagag attgaccgcg tcgtagccgg
     3121 cctcgaagcg cggcgtgtag tcggccgggc tcatccggtt gccgggaccg gtccggatgg
     3181 ttacagtcga aaagtccggc ccgcggccgg ccacctcgtc aagaccggcg gcggcctggg
     3241 cggccgccag gctgaaggtg aaggggaagt caatgctgaa atcggtgccc cgggccatca
     3301 cggccaggtc aaggacacgg ccgcccgggg tgccggtcag gagcagaatc agccgccggt
     3361 tttcctttcc ctgaatctcc aggttctgca gttcggccgg gttggcgaag aagccgccca
     3421 ggcggccgcc caggataatc gagacccgct gcaggctctc cttttcgtta tgaagcagca
     3481 ggttgagggc aaacccggtt ccggtcggtg ccggcaccag gcgggcgtca acgttgaacc
     3541 agccgcgcac cgtgccccgg tcaagcatcc cggcgacctg cgccggcggc gcggcgaccg
     3601 tgaagtacct cgggaaacga tccacctgga aactgccgtc cttccccagg gccagcttat
     3661 tcccgcacca gtcgtagacc tgccagccgc cggccgtttc gggccggatt tccaggagac
     3721 gtccgtccac gctccagacc accccaaccg tctggccgcc cttttcgaag aggccgagcg
     3781 tcacccgcgg gtcgccccgc cactcggcgt aatggcgggc atcctccagc aggtggctgg
     3841 cgatggagcg gccgaccgcg taagggcgca ggctgccgtc gtactcgacg tcctccttgc
     3901 cgccgccggg ccgccgggtc gtttcggtgg gggcggtgtt gccaaaacgg ctgaagtaat
     3961 ggaagtagtg gtcaatggcc ccgatggcga aacagcgcaa ttctccctgg agggtccgga
     4021 tgttgctgtc gcggacgccc tgactgctgg gattggcgtg gtgctcctcc ccgtaaagct
     4081 gctgaagggt gggcagcgtc ttgtaaaagg aaggaaaggt cggcccggcc tcggtctgcc
     4141 agccgctctt gtcgtatttt ttgaggaccg aggcgaagta gcggagcttg accgggtcgg
     4201 tgtcgtagaa gtggccgctg acgccgtcgt accacttcgg atcggccgcc cggaccgccc
     4261 gcggaaagta ggccggcatg ttgccggagc agaaaccgat cacctttatg ccgggatcga
     4321 cctgccggat ccgctcgctg gccacccgca ggatgtcggc gtaatcctcc ggcgtgcacc
     4381 agaagtaggg ttcgttggtc acctcccagt ggcggatctt ctcccggtaa tggctgacca
     4441 ggtcgtaaac gtaattcccc cagatttcga ggtacctttt cttcttctcc ggctcccagc
     4501 cctggttggt gccgcaatac ggcttcaggt aggccggcgc attgttgacg aagacctggc
     4561 ccagcatcat gattttggcg tccaggacat tctggagcgc ctcgtccgac cagcggtatt
     4621 tgcccggttc aggcatcacg ccttccatcc ggccgatgcc cttgctgatc acccagttgc
     4681 agttggcccg cttcaggatt gccagcgcgt ttggatcgga tccggtgtcg gttcccatcc
     4741 ggccgggttc gtagaggata ttcaggtggc ggttgggcgg cagtatggaa aaactgagtt
     4801 cgtccggtcc ggccgcgtcc tcccgggtcc aggccagcat ccggaagatg ccccggcggt
     4861 cgagcggcac cgccggtttc cggctcagat gggtttcgcc ccggagcggt atccggaagg
     4921 tctcctccct gaccggccgg tcgaagtaat cctcgatccg ccaggttgcc tccagcgtgc
     4981 cggtattctt gccggggttg taaaccagca gttccaggtt ggccggctcg ttctcgtaaa
     5041 ggatgtggcc gggctgccgg cagaggatgc cgacctcgac gccgccccgg ggtcggtaag
     5101 ggctggccgt ttcggcccgt tccagctgga gggcgtccac ccagacgttt tggggtatcg
     5161 gaaggtcggc cttccggatc tcctccaggc tcgaatcaac cttgaatccc aggttgatgg
     5221 tgcgccactc catgttcagg ccgacggccc agaagttgcc cggtccctcg ggaacgtcga
     5281 aggtaaaggc aaaacgcttc cactcggtgg tgagatcggc ccggccgccg ccggccgtcc
     5341 gccccggacc gtcctgggtg gccttctccg gatggccgaa gaggcgcagg ttgacgctgc
     5401 aggctttgtc ggccttcagg taggcagaga gggtgtagcg gcccggcggc agccggtaga
     5461 acttgctctc gaagccgctc tggttgttga gacggtagta gtagcccagg gtcgagtacc
     5521 acatctggat gaccggcagc cgcagcgagt aacggccgtc ggcggcggtg ctgtcgtccc
     5581 actggttgat ccagaccttg ccccagccgt ggccgatgcc cagttcgaat gaggagttct
     5641 ccaggtagtt gtcggtgcgc agcgggggcg gcggaacctg ccccggggcc aggaccggcc
     5701 ccgccccgga ccggatgatg tcatcccggc cgattatttc gttgaggtcg gtggtgatat
     5761 agattccctg cagtatgata ttctgagtcg ggctctgttc cggcttgaga gtaggctgga
     5821 aggtgagtgc caattccggg gccggcgcgg tgatggttag taccaggccc gaccagccgg
     5881 cgctcacgtg gcgggcatag gtgagcggtt cgctggtcgt tccggccagg ccgaagctga
     5941 cccggccgcc gtggaactcc ttgatgagaa ttttgtaggt tccggccggc aggggcgggt
     6001 tgatgggcag acgataaacg gccttcgccc cggccgccgg gtcggtggcc atgccactca
     6061 tggcgcccgg caccgaatct ccgcccccgc tgtaactgcc ccagcccgac ttcccggcca
     6121 gcatggcgcc ggtccacatc cggctgatac tcttctcctg ggccgttagc ggcccggcgg
     6181 cggccgccag gcagccgatc gccacgagga agccaagcca tcgtctcatc ttcttccctc
     6241 cggttggggt aaaatccgtt cggaaaaggg tccgattacg attgactgaa acatcggctg
     6301 gaagtgaatg ccgccccctt tcctactcgc cgaaacgggt cagttggcgg cttccagtcg
     6361 catcgtcttg agtgcggcgg cggccgcggc cgggtcggcc ggcccggtga agtagaccgg
     6421 gctctcggtc agtcgcaggc cggccggctc gatcggattg cccatcaggt cgtagaagtt
     6481 gaagccggcc ggcgggaccg ccttgaagac cttcgcctcg gtcacccaga agaa
//
LOCUS       DAOQYN010000106         6515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_12220_length_6515_cov_4.106037, whole genome shotgun sequence.
ACCESSION   DAOQYN010000106 DAOQYN010000000
VERSION     DAOQYN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12220_length_6515_cov_4.106037"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PKN80_05280"
     CDS             complement(<1..1020)
                     /locus_tag="PKN80_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNQ35462.1"
                     /translation="MNTADGGAVSRSERLAWFNQARFGLFIHWGLYSLLKRGEWVMYV
                     ERIPAAGYAKLARRFQPKHFDADAWLQRARAAGMRYAVLTTRHHDGFCLFDSRASDFT
                     SVRTAAGRDFVAEYVEACRRAGLKVGLYYSLLDWRFPGYFNREKYPESFEAMVEQAHA
                     QVRELLTNYGPIDILWYDGGWIPGVERTEMARLWRAAELNAAARRLQPGIIINNRSGL
                     DEDIDTPEQHVTASAAGRAWETCMTMGDFCGWGYIRNNPNFKTATQLIQHLVQAAAGG
                     GNFLLNIGPRPDGTLRNKEIRRLEEIGAWMQANGASIYGSELLPKGWGGAWGGGMAGT
                     MTAVGR"
     gene            1156..1611
                     /locus_tag="PKN80_05285"
     CDS             1156..1611
                     /locus_tag="PKN80_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HNQ35463.1"
                     /translation="MIEGVKVKRLKVVPDERGRLMEILRSDDELFSRFGQVYLTTVLP
                     GVVKAWHCHKKQTDNLAVVRGMMKVVLYDNRPNSATRGQLNEFFAGEHNPILIQVPPE
                     VYHGFKCVGAEEALAINIPTEVYNYHEPDEHRLDPHSGEIPYSWERVDR"
     gene            1608..2333
                     /locus_tag="PKN80_05290"
     CDS             1608..2333
                     /locus_tag="PKN80_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ35464.1"
                     /translation="MKGVVLAGGLGSRLRPLTKITNKHLLPVYDQPMIYYPLRRLVEA
                     GITEILLVTGGVYAGEFLRLLGNGAQFGLKHLHYTYQEGEGGIAAALALAEDFADGDR
                     IAVILGDNIFQEPIKPYVKNYRRQAQGARILLAEVANPERFGCPELKKGKLVRIVEKP
                     AAPASRYAVTGLYFYDREVFDIIRQLKPSARGELEITDVNNHYLKKGRLEYDRLAGWW
                     TDAGAFDSLLHASNLVAADRRRK"
     gene            2330..3292
                     /gene="rfbB"
                     /locus_tag="PKN80_05295"
     CDS             2330..3292
                     /gene="rfbB"
                     /locus_tag="PKN80_05295"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNQ35465.1"
                     /translation="MKLLVTGGCGFIGSNFILHVLRSHPGDRVVNLDKLTYAGNPANL
                     AEIKGDPGYEFVRGDICDARLAAEVLAGCDAVVNFAAESHVDRSIREPDPFLLTNFIG
                     TGVLLEAARRLKIPRFLHISTDEVYGPMDSGQADESAPLHPSSPYSASKAAADHLCLA
                     YHATYRLPVLVTRSSNNYGPRQYPEKLVPLCILRALAGGELPLYGDGQNRRDWLYVED
                     NCRAIDLLLRQGREGEIYNIAGGRELSNLEVAGRILELAGRPPARVTFVADRPGHDRR
                     YGINWEKLKGMGWRPEVSFEEGLARTLDWFRKNPGREARRPNGK"
     gene            3289..4140
                     /gene="rfbD"
                     /locus_tag="PKN80_05300"
     CDS             3289..4140
                     /gene="rfbD"
                     /locus_tag="PKN80_05300"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNQ35466.1"
                     /translation="MKRLKLLVTGHRGLLGSDLAAGLAKEYEVTGIGRAECDLTRRPA
                     VLEALKRLRPDLAVHAAAWTDVDGCEREPETAYRVNVLATGHLAEACQRLKIPLLYIS
                     TDFVFDGRKKSPYRESDPTNPIQVYGRSKLAGETAVQNLCERFFIVRTAWLFGRHGRT
                     FPARILEAARRGETAPVIRNQTGSPTYTVDLTRAVGEILRGGGYGLYHAVNLGAASRL
                     ELAREVLKQAGLKGRLKPVTAAELKLPARRPARSALRTLVLEAETGLKMAEWPEALHR
                     YLAEETD"
     gene            complement(4144..4878)
                     /locus_tag="PKN80_05305"
     CDS             complement(4144..4878)
                     /locus_tag="PKN80_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ35467.1"
                     /translation="MWRDRMVSVILPTYNEKDSIRRVVDEFFATGVVDEVIVVNNNAR
                     EGTSAEVARTRAREVLEPRQGYGAAIQRGFREARGELLVLSEPDGTFAAHDIFKLLAY
                     SDDFEAVFGTRTTSVLIWAGANMGPLLKWGNWAVAKMLEFLFNTTTFTDVGCTMRLIK
                     RPALEKIRSQFTVQREHFGPEMMILAVINRLRYIEVPVNYLPRVGVSSVTGHKWRTVQ
                     LALRMVALILYYRVAVWLGRKPRPVS"
     gene            complement(4868..6127)
                     /locus_tag="PKN80_05310"
     CDS             complement(4868..6127)
                     /locus_tag="PKN80_05310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ35468.1"
                     /translation="MKRMLLIIFLLGLALRVLWIASLDNRVDDWGEEGVKEAAWSLIE
                     GRGYSMPRSVSCYPGDTPLYSWREPGFSLILVPVFRLFGENYLAVKILLAILSSLTAV
                     FTYLLGRDLFDSEPVARLAAFGMAVLPESIHWTGYLTPESVTIFVLILPVFFLVRGLR
                     RPAPVNPAAAGLLLGVAALTRAQAIVLMPFLLAAYFIGRRDPRRAGREVFLAGGLALL
                     VFSPWVIRNYRIHKQLVVMPTVAGEVLYIANNPGAVRMMKTPAGFFHGEEPERRFIGR
                     SELEISRWYRRAALGFILSRPGDYARLAADRFVRFWRFYPHRGVGVSGRYYNILHLLV
                     SLLTSGVAITLFLPGVFLSRRHWRRTLIPLVLVVVFSTLTVMARATIRYRLPLMPYVM
                     IFAAFAFVRLVEWRRRGPVPREVGHVA"
     gene            6299..>6515
                     /locus_tag="PKN80_05315"
     CDS             6299..>6515
                     /locus_tag="PKN80_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35469.1"
                     /translation="MARRMVSAIMVLLLLALWCSAGEAGMREAEELYRQKKFVEAEAE
                     IRKVLQEKPDAGGGTLQLLLGHTLNAQK"
BASE COUNT         1279 a         2152 c         2098 g          986 t
ORIGIN      
        1 gcggccgacc gcggtcatgg tgccggccat gccgcccccc caggcgccgc cccagccctt
       61 gggcaaaagc tccgagccgt agatggaagc gccgttggcc tgcatccagg cgccgatctc
      121 ctccagccgc cggatctcct tgttccgaag cgtaccatcg ggccggggac cgatgttgag
      181 caggaaattg ccgccgccgg cggccgcctg gaccaggtgc tgaatcagct gggtagcggt
      241 cttgaagttc ggattgttcc ggatgtagcc ccagccgcag aaatcgccca tcgtcatgca
      301 ggtctcccag gcccggccgg cggccgaggc ggtgacgtgc tgttccggag tgtcgatatc
      361 ctcgtccaga ccagagcggt tgttgatgat gatccccggc tggagacgcc gggcggccgc
      421 gttcagctcg gcggcgcgcc agagccgagc catctcggtg cgttccacac ccggtatcca
      481 gccgccgtcg taccagagaa tgtcgatcgg gccgtaattg gtcagtagtt cccgcacctg
      541 ggcgtgggcc tgctcgacca tcgcctcgaa gctctccggg tacttttccc gattgaagta
      601 accggggaaa cgccagtcca gcagtgaata gtagaggccg actttcaacc cggcccggcg
      661 gcaggcctcg acgtactcgg ccacgaaatc ccggccggcg gccgtccgga ccgaggtgaa
      721 atcgctggcc cggctgtcga agaggcagaa cccgtcatga tggcgagtgg tcaggaccgc
      781 gtagcgcatc ccggccgccc gggcccgctg cagccaggcg tcggcgtcga aatgcttcgg
      841 ttggaagcgc cgggccagct tcgcgtaccc ggcggccgga atccgctcga cgtacatcac
      901 ccactcgccc cgcttcagaa gcgagtaaag tccccagtgg ataaagaggc cgaaacgcgc
      961 ctggttgaac caggccagcc gttccgacct ggagaccgcc ccgccgtccg ctgtattcat
     1021 tcccgtcccc cgtttggcaa gcgactacag aaagacgatg aagttcaagc ggatatttta
     1081 cccggatcat cccggttcgg caaacgccga tttcatcttt acagggtcaa aaggacgggt
     1141 tataattgaa ccaccatgat tgaaggcgta aaggtcaagc ggctcaaggt cgtcccggac
     1201 gaacggggac ggctgatgga aatcctccgg tccgacgacg aactcttcag ccgtttcggc
     1261 caggtctacc tgaccaccgt cctgcccggg gtggtcaagg cctggcattg ccacaagaag
     1321 cagaccgaca acctggcggt ggtccgggga atgatgaagg tcgtcctcta cgacaaccgg
     1381 ccgaactcgg ccacccgcgg ccagctgaac gaattcttcg ccggggagca caacccgatc
     1441 ctgatccagg tgccgccgga ggtctatcac ggcttcaagt gcgtcggcgc cgaagaggca
     1501 ctggcgatca acattcccac cgaagtttac aattaccacg aaccggacga gcatcggctc
     1561 gacccgcact ccggcgaaat cccctactcc tgggaaagag tcgaccgatg aagggcgtgg
     1621 tcctggccgg gggattgggc agccgcctcc ggccgctcac caagatcacc aacaagcacc
     1681 tcctgccggt ctacgaccag ccgatgattt attacccgct ccggcggctg gtggaggccg
     1741 gcatcaccga gatcctgctg gtgaccggcg gcgtctatgc cggcgagttc ctgcggctgc
     1801 tgggcaacgg cgcccagttc ggcctgaagc accttcatta cacctaccag gagggcgaag
     1861 gcggcatcgc ggccgcgctc gccctggccg aagacttcgc cgacggggac cggatcgcgg
     1921 tcatcctggg cgataatatc tttcaggaac cgattaaacc gtacgtaaaa aattaccgcc
     1981 gccaggccca gggggcccgg attctcctgg ccgaggtcgc caacccggaa cgcttcggct
     2041 gcccggagct gaagaaggga aagctggtcc ggatcgtcga gaagccggcg gcgccggcca
     2101 gccgctacgc ggtcaccggc ctctactttt acgaccggga ggtcttcgac atcatccgcc
     2161 agctgaagcc ctcggcccgg ggtgagctcg agatcaccga cgtcaacaac cattacctga
     2221 agaaaggccg gttggagtac gaccggctgg ctggatggtg gaccgacgcc ggcgccttcg
     2281 attcgctcct gcacgccagc aacctggtgg ccgccgaccg gcggcgaaaa tgaagctcct
     2341 ggtcaccggc ggctgcggct tcatcggctc caacttcatc ctgcacgtct tgcgcagcca
     2401 tccaggcgac cgggtggtca acctcgacaa gctcacctat gccggcaacc cggccaacct
     2461 ggccgaaatc aagggtgatc ccggctacga gttcgtccgg ggcgacatct gcgacgcccg
     2521 gctggcggcc gaggtcctgg ccggctgcga cgcggtggtc aacttcgccg ccgaaagcca
     2581 tgtggaccgc tcgatccggg aaccggatcc cttcctgctg accaatttta tcgggaccgg
     2641 ggtgctgctg gaggcggccc ggcgcttgaa aatcccccgc ttccttcaca tttcgaccga
     2701 cgaggtttac ggcccgatgg attcgggcca ggccgacgag tccgccccgc tccacccctc
     2761 ttcaccctac tcggccagca aagccgccgc cgatcacctc tgcctggcct atcacgccac
     2821 ctaccggctg ccggtgctgg taacccgcag cagcaacaat tacgggccgc gccagtaccc
     2881 ggagaaactg gtgccgctct gtatcctccg ggcgctggct ggcggcgagc tgccgcttta
     2941 cggcgacggc caaaaccgcc gcgactggct ctacgtggag gacaactgcc gggccattga
     3001 cctgctcctg cgccagggcc gggaaggaga gatttacaac atcgccggcg gccgggaatt
     3061 gagcaacctg gaggtggccg gccgcatcct ggaactggcc ggccggccgc cggcccgggt
     3121 aaccttcgtg gccgaccgcc ccggccatga ccggcgttac ggaatcaact gggagaaact
     3181 gaagggaatg ggctggcggc cggaggtctc cttcgaggaa ggcctggccc gcaccctgga
     3241 ctggttccga aaaaatccgg gacgggaagc gcggcggccg aacgggaaat gaagcggctg
     3301 aaattgctgg taaccggcca ccgcggcctg ctgggaagcg acctggccgc cggcctggcg
     3361 aaggagtacg aggtaaccgg aatcggccgg gccgaatgcg acctgacccg ccggccggcg
     3421 gtgctggagg ccctgaagcg tctccggccc gacctggcgg tccacgccgc cgcctggacc
     3481 gacgtggacg gctgcgaacg ggagccggag accgcctacc gggtcaacgt cctggccacc
     3541 ggccacctgg ccgaggcctg ccagcggctg aaaatccccc tgctttacat ctcgaccgat
     3601 ttcgtcttcg acggccgaaa aaaatccccc taccgggaat ccgatccgac caaccccatc
     3661 caggtttacg gccgctccaa gctggccggc gaaacggccg ttcagaatct ctgcgaacgg
     3721 ttcttcatcg tgcgcaccgc ctggctcttc ggccggcacg gccgaacctt cccggcccgg
     3781 atcctggaag cggcccgccg gggcgaaacc gccccggtca tcaggaacca gaccggttcg
     3841 cccacctaca cggtggacct aacccgggcg gtcggcgaaa tcctgcgcgg gggcggttac
     3901 ggactctacc acgcggtcaa tctcggcgcg gcctcgcgcc tggaactggc ccgggaggtt
     3961 ctgaaacagg cggggctgaa aggacggttg aagccggtga cggccgcgga gctgaaactg
     4021 ccggcgcgac ggccggcccg gtcggccctg cggaccctgg tcctggaggc cgaaaccggc
     4081 ctgaagatgg ccgagtggcc ggaggcgctc caccgttacc tggccgaaga aacggattga
     4141 atctcaagaa accggccgcg gcttgcgacc caaccagact gccacccggt agtagagaat
     4201 cagggccacc atccgcaggg ccagctgcac cgtccgccac ttgtggccgg taaccgagga
     4261 gacgcccacc cggggcaggt agttgaccgg cacctcgatg taacgaagcc ggttgatgac
     4321 cgccaggatc atcatctccg gtccgaaatg ctcccgctgg accgtgaact gggagcggat
     4381 cttctccagg gccggccgct tgatcaggcg catcgtgcag ccgacgtcgg tgaaggtggt
     4441 ggtgttgaag agaaactcca gcattttggc caccgcccag tttccccact tgagcagcgg
     4501 ccccatgttg gcgccggccc agatcaggac cgaggtggtc cgggtgccga agaccgcctc
     4561 gaaatcgtcg gagtaggcca gcagcttgaa gatatcgtgg gcggcgaagg tcccgtcggg
     4621 ctcggaaaga accagcagtt cgccccgggc ctcgcggaaa ccgcgctgga tggccgcccc
     4681 gtaaccctgg cgcggctcga ggacctcgcg cgcccgcgtc cgggccacct cggcgctggt
     4741 gccttcccgg gcgttgttgt tgaccacgat cacctcgtca accacgccgg tggcgaagaa
     4801 ctcgtccacc acccgccgga tggaatcctt ctcgttgtag gtcggcagga tcaccgacac
     4861 catccggtca cgccacatgg ccgacctccc gcggaaccgg accccggcgg cgccactcaa
     4921 ccagccggac aaaagcgaag gcggcgaaga tcatcacgta aggcatcagc ggcaaccggt
     4981 agcggatggt cgcccgggcc atgacggtaa gggttgagaa gacaaccacc aggaccagcg
     5041 gaatcagcgt ccgacgccag tggcgccggg agaggaagac gccgggcagg aagagcgtga
     5101 tggccacccc ggaggtcagc aggctgacca gcaggtgcag gatgttgtaa tagcggccac
     5161 tcacgcccac gccccggtgg ggatagaaac gccagaaacg cacgaaccgg tcggccgcca
     5221 gccgggcgta gtcacccggc cgggacagga tgaagccgag ggccgcgcgg cgataccaac
     5281 ggctgatctc cagttcggag cggccgataa accggcgttc gggctcctcg ccgtggaaga
     5341 acccggccgg cgtcttcatc atccggaccg cgcccggatt gttggcgatg tagagaacct
     5401 cgccggccac cgtcggcata accaccagtt gcttatggat gcggtaattc cggatgaccc
     5461 agggtgaaaa gaccagcaag gccaggccgc cggccaggaa gacttcccgg ccggcccgcc
     5521 gcgggtcccg gcggccgatg aaataggcgg ccagcagaaa gggcatcaac acgatcgcct
     5581 gggcccgggt cagggcggcc acgccgagca gcaggccggc ggccgccggg ttgaccggcg
     5641 ccggccggcg gagtccacgc accaggaaga agaccggcag gatgagcacg aagatggtga
     5701 cgctctccgg cgtcaggtag ccggtccagt gaatcgactc aggcaggacg gccattccga
     5761 aggcggccag ccgggccacc ggttcagagt cgaagagatc ccgccccagc aggtaggtga
     5821 agaccgccgt caagctggag agtatggcca gcaagatctt gaccgccagg tagttctcgc
     5881 cgaagagccg gaaaaccggg accagaatca gggaaaaccc gggctcccgc caggagtaga
     5941 gcggggtatc gcccggatag caggagaccg agcggggcat gctgtagccg cgcccctcga
     6001 tcagggacca ggccgcctcc ttgaccccct cctcgcccca gtcgtccacg cggttgtcaa
     6061 gcgaggcgat ccagaggacc cgcagggcca gcccaagcag gaagataatc aggagcattc
     6121 gtttcatcgg gtttcctaat aaggatagct gcccggtctg acattcaaaa accggacacg
     6181 gatattttaa ccgttgcccg gcttttaggc aaaaaacact tgacttcccc ggaaggccgg
     6241 tgttatcgtt aaaaccggcc ggttgataaa tcattttcaa acgaaaaggg aggcggagat
     6301 ggccaggaga atggtatcgg caataatggt gctgctgctg ctcgcgctct ggtgctcggc
     6361 cggggaagcc ggcatgaggg aggccgaaga gctttaccgg cagaagaagt tcgtcgaggc
     6421 cgaggccgag atccgcaagg ttctccagga gaagccggat gccggcggcg gaaccctaca
     6481 gctcctgctc ggccataccc tgaacgccca gaaaa
//
LOCUS       DAOQYN010000107         6501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_12261_length_6501_cov_3.672355, whole genome shotgun sequence.
ACCESSION   DAOQYN010000107 DAOQYN010000000
VERSION     DAOQYN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12261_length_6501_cov_3.672355"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKN80_05320"
     CDS             <1..190
                     /locus_tag="PKN80_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNQ35470.1"
                     /translation="KIILVALGGGRAMAIARALEGPLTSMVTASVFQRHPDCTVVLDR
                     EATIHLKKKNAYEEINAT"
     gene            208..534
                     /gene="hisI"
                     /locus_tag="PKN80_05325"
     CDS             208..534
                     /gene="hisI"
                     /locus_tag="PKN80_05325"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006055644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNQ35471.1"
                     /translation="MEGVKLGQVKFNDQGLVPVVAQDRDGRVLMLAYANLEALRLTLA
                     TGRAHYYSRSRQQLWRKGESSGNEQVIEAVLVDCDQDTILYRVRQSGPACHTGNPTCF
                     FRRLET"
     gene            complement(553..1524)
                     /locus_tag="PKN80_05330"
     CDS             complement(553..1524)
                     /locus_tag="PKN80_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNQ35472.1"
                     /translation="MSTRPAAGRWLLLFLLVTAGLSGCGRPPVRHYLSPELEAGLTRY
                     SDLPARLVELVDSAREEVLVATYEAQLPSVLTALDQARRRGVSVRMLQDDATLGDTGF
                     FGPVTTDRNRYLMHAKFMVVDRRTVWIGSANLTFSSVYRNDNDYLIIRSSGLADYLAG
                     AFQLMLDGRVPGRPFQDAAGLRPDCRVYLNPWCRAALLEELTAARRHIFFSLYALTDP
                     EVIRLLGARSAAGVRVEGILERDWPMNRAAYEKLRRSGVDVRWDGNPNLNHYKLFVID
                     RARVVTGSYNPTRTAPKNQEVMAVFPDSGLAGFYLRNLAFGWGEPRP"
     gene            complement(1532..2899)
                     /gene="mgtE"
                     /locus_tag="PKN80_05335"
     CDS             complement(1532..2899)
                     /gene="mgtE"
                     /locus_tag="PKN80_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNQ35473.1"
                     /translation="MEREKVSGMRLILPEIRELLSHKEVPENLDEILEEFNPVDVAEA
                     MPELEPIERVRLLSRLRPDFAGEIFSYLGPDDQLAILEGLEPQRTRALLSEVEPDDRA
                     DLFEELPPELKARFLSIMENEAARETRELLQYPSQSAGGLMTPRFLEVKEGDTVSDAL
                     DRIRRTGQEIEQAHHIFVTDSDGRLVGHVSLRGLVMAAPQLRVSEIMNENPVAVTVSQ
                     DQEEVARQMRKYDLVVMPVVGADRRLKGVVAIDDAMDVLTEEQTEDMYKFGAVAGGFV
                     DSYSGQNPFSIVRHRIPWLVMLVLLGFISGFVIDHYSLLMQQVIALAVFIPLLMGAAG
                     NAGTQASTVMVRGLATGEVRLRNLWRVVEKELLVGLMAGLILGLLAVLRVMVLREGLM
                     IALVVAVSMTAVVALATTIGAALPLICKRFGLDPAVVSSPLLATVMDISTLFIYFEIG
                     RRLLF"
     gene            3176..4627
                     /locus_tag="PKN80_05340"
     CDS             3176..4627
                     /locus_tag="PKN80_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ35474.1"
                     /translation="MNRRDFIKFSSVSLPGALLLSALKPHPGMAADASPERTEIAFGA
                     PGKPTASYIMEPRRRLPVAAETDVLVIGGGPAGFPAAIAAARGGARTMLVERHPCLGG
                     NLTAGSVINIRTYNDQAGRLIIQGIPLEFARRLKEMGGTASDPVKEPYVRQEPEASKY
                     LIQEMALEAGVELRLYSLAVDVIKKGNQLRGVVLESKSGRQAVLAKAVIDATGDGDIA
                     ASAGAPYEKGWPGNEQLLQPLTLAFVIANISQYLRDHWAEEYPKLNAIMKRAYQNGAY
                     PVKRQPGGLFPMAGRRELYANATRMAGDTTDVQDLTRAEIEGRRQAWALMKFYRDNLP
                     GFEETYLRYTASQAGPRESRRILGDYVLNREDVLEGRDFPDGVARGAYTIDIHNPKDG
                     TTQYLRLPPGTSYAIPYRCLVPQRVENLLVAGRCISVNHEALGSIRVMATCMATGQAA
                     GTAAALAVQAGTTPRRLPVVRIQKRLQADGAVI"
     gene            4660..5238
                     /locus_tag="PKN80_05345"
     CDS             4660..5238
                     /locus_tag="PKN80_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNQ35475.1"
                     /translation="MKRFDSAGILAFGREETLTVRLLLSLTLAGVTGLLAGIRVPLPF
                     TPIPLTGQVLGVYLAGLLLDAPFAGLSLLFYLVLGLAGLPWFAGWQGLNYAGFLAYPS
                     AGYLLGFIPAAFLIGRLLEDPARRSLARQVLAYSAGTAVFYFCGAAWLAALTGAGFIR
                     TLAMSVYPFVIFDLLKALMVASFASALIGLRR"
     gene            5240..5659
                     /locus_tag="PKN80_05350"
     CDS             5240..5659
                     /locus_tag="PKN80_05350"
                     /inference="COORDINATES: protein motif:HMM:NF018621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HNQ35476.1"
                     /translation="MRLRGHHLLCLLGFRGLGYSPEFVARMQEVRSALNSKRRFFIQV
                     VTESDDICRACPYHSNNACRKGSLSVRRTAEMDRRTLQYLGLQPGRRISADRLLKIIP
                     QRLGDFDRLVKICGRCGWREVCNYYLELRSRWRRSAL"
     gene            5708..6289
                     /locus_tag="PKN80_05355"
     CDS             5708..6289
                     /locus_tag="PKN80_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ35477.1"
                     /translation="MGDYETILKRRTIRRFRPDPVPPELLEKLAEAGRLAPSGGNLQP
                     CEFIIVDQPDRVAELFPALRWAAYVAPAGNPPEGHRPTAYVVVLVNQSIKREGPVDSA
                     AAVMSMILTAQEAGVSSCWLGSIEREKLAEMLGIPGNYHIDSVLALGYPDQKSCVEEY
                     RDSVKYWLDDRGDFHVPKRGLKEILHRNAFGRR"
     gene            6344..6428
                     /locus_tag="PKN80_05360"
     tRNA            6344..6428
                     /locus_tag="PKN80_05360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6378..6380,aa:Ser,seq:gga)
BASE COUNT         1260 a         2178 c         1947 g         1116 t
ORIGIN      
        1 gaaaataatc ctggtggcgc tcggcggcgg ccgggcgatg gccatcgccc gggcgctgga
       61 ggggccgctt accagcatgg tcaccgcctc ggtcttccag cgtcatccgg actgcaccgt
      121 ggttctggac cgggaggcga ccattcactt gaagaaaaag aacgcctacg aagagatcaa
      181 cgcgacatga aaaaagaaaa cgggatcatg gaaggagtga aactcgggca ggtcaagttc
      241 aatgaccagg gcctggttcc ggtcgtggcc caggatcggg acgggcgggt gttgatgctg
      301 gcctacgcca acctggaagc gctccggctg acgctcgcca ccggccgggc ccattactac
      361 agccgttccc gccagcaact ctggcgcaag ggcgagtcga gcggaaacga acaggtaatc
      421 gaagcggtcc tggtcgactg cgaccaggat accatcctct accgggtccg gcaaagcggg
      481 ccggcctgtc ataccggcaa cccgacctgt ttcttccgcc gcctcgaaac atgaccagcc
      541 gccggccgta aattacggcc ttggctctcc ccaaccgaag gcaaggtttc gaaggtaaaa
      601 cccggccagc ccggaatcgg gaaagaccgc catgacctcc tggttcttcg gggcggtccg
      661 ggtcggattg taggaaccgg tcaccaccct ggcccggtca atcacgaata acttgtagtg
      721 gttgaggttc gggttcccat cccacctgac gtccacgccc gaccggcgca acttctcgta
      781 agccgcccgg ttcataggcc aatcccgctc caggatcccc tcaactctta ccccggcggc
      841 cgacctggct cccagcagcc tgatcacctc cgggtcggtc agggcgtaaa gggaaaagaa
      901 gatatgacgg cgggcggccg tcagttcctc cagcagggcg gcccggcacc aggggttcag
      961 gtaaacccgg caatccggcc ggaggccggc cgcgtcctga aaagggcgcc ccggaacccg
     1021 cccatccagc atcagttgaa acgctccggc caggtaatcg gccaggccgg aggaacgaat
     1081 gatcaggtag tcgttgtcgt tccggtaaac cgaggaaaag gtaaggttgg ccgaaccgat
     1141 ccagacggtc cgccggtcca ccaccatgaa tttcgcgtgc atcaggtagc ggttgcggtc
     1201 ggtcgttacc ggtccgaaga aaccggtgtc gccgagggtg gcgtcatcct ggagcatccg
     1261 gaccgaaacg ccccgccgcc gggcctgatc cagggccgtc agaaccgagg gcaactgcgc
     1321 ctcgtaggtg gccaccagaa cctcttcccg ggcggaatcg accagttcca ccagccgggc
     1381 gggcaggtcg gagtaccggg tcagaccggc ttccagttcg ggggagagat aatggcgcac
     1441 cggcggccgg ccgcacccgg acaatccggc cgttaccagc aggaaaagga gcagccagcg
     1501 gccggcggcc ggtctcgttg acatgccggg ttcagaagag caggcggcgg ccgatctcga
     1561 agtaaatgaa gagcgtggag atatccatga cggtggccag cagcgggctg gagacgaccg
     1621 ccgggtccag gccaaaccgt ttacagataa gcggcagggc cgccccgatc gtggtcgcca
     1681 gggccaccac cgcggtcatc gaaacggcca cgaccagggc gatcatcaag ccttcccgca
     1741 aaaccatgac ccggagcacg gccagcagac ccaggatcaa gccggccatc aggccgacca
     1801 gcagctcctt ttcgaccacc cgccagagat tgcgcaaccg gacttcgccg gtggccaggc
     1861 cgcgcaccat caccgtgctg gcctgggtgc cggcgttacc agccgccccc atcaggagcg
     1921 ggatgaaaac ggccagggcg atcacctgct gcatcaggag tgaataatgg tcgatcacga
     1981 aaccggagat gaagccaagc aggacaagca tgaccagcca cggaatgcgg tggcggacga
     2041 tggagaaagg attctggccg ctgtagctgt ccacgaaacc gccggcaacc gccccgaatt
     2101 tatacatatc ctcggtttgc tcttcggtca gcacatccat cgcgtcatcg atcgccacca
     2161 ctcccttgag ccgacggtcc gcgcccacca ccggcatcac caccaggtcg tacttgcgca
     2221 tctggcgcgc cacttcctcc tgatcctggc ttacggtcac ggccaccggg ttctcattca
     2281 tgatctccga aacccggagc tgcggcgcgg ccatcaccag tccgcgcaac gaaacgtgac
     2341 cgaccagcct gccatccgaa tcggtgacga aaatatggtg ggcctgctcg atctcctggc
     2401 cagtccggcg aatccggtcc agggcatcgc tgaccgtgtc cccctccttg acctccagaa
     2461 aacgcggggt catcaggccg ccggcgctct gagacgggta ttgcagaagc tccctggttt
     2521 cccgggccgc ctcattctcc atgatggaga gaaagcgggc cttcaattcc ggcggcagtt
     2581 cctcgaacaa atcggcccgg tcatccggct ccacctccga aagcaacgcc cgggtgcgct
     2641 ggggctccaa accctccagg atggccaatt gatcatcggg gccgagatag gaaaatattt
     2701 cgccggcaaa atcgggccgg agcctcgaca ggagccggac ccgctcgatc ggttccagtt
     2761 ccggcatcgc ctcggccacg tcaaccgggt tgaattcttc caggatctcg tccaggttct
     2821 ccggaacctc tttgtggctc aggagttccc ggatctccgg caggatcagc cgcatcccgg
     2881 atactttttc ccgttccatg ctggcctctt tttatggtcg ctgaaacagc tgtcgtcggt
     2941 ttttattata ccacctggat cggcaaccgc acccgggcgg ggaaccaccg gccgcaaaac
     3001 gttctttcca cccccttgtc tatgatataa taaatcaacc cccggccgat tttcccgaag
     3061 gaatctgttg gccgccggag ggccggaccg gttgccgttc agccatcccg gccggtggtt
     3121 tcaagataag cgtttttgct ttttcctgcc atttcatgcc gaaagggaga acgggatgaa
     3181 ccgtcgcgat ttcattaaat tctcatcggt ctcgctgccc ggcgcccttc tgctgagcgc
     3241 cctcaaaccg catccgggga tggcggccga cgcttctccc gaaaggaccg aaattgcctt
     3301 cggcgcgccc ggaaaaccaa cggccagcta cataatggaa cccaggcggc gcctgccggt
     3361 ggccgccgaa accgacgtgc tggtgatcgg cggcggcccg gccggatttc cggccgccat
     3421 cgccgcggcc cgcggcggcg cccgaaccat gctggtcgaa cgccacccct gcctgggcgg
     3481 caaccttacc gccggaagcg tcatcaacat ccgcacctac aacgaccagg ccggccgcct
     3541 catcatccag gggattccgt tggaattcgc ccgccggctg aaagaaatgg gcggcaccgc
     3601 ctccgacccg gtcaaggaac cttacgtccg ccaggaaccg gaggccagca agtacctgat
     3661 ccaggagatg gccctcgaag ccggcgtgga actccgcctg tactccctgg cggtcgatgt
     3721 catcaaaaaa gggaaccaac tccggggggt cgtcctggag agcaagtccg gccggcaggc
     3781 ggtactggcc aaagcggtaa tcgacgccac cggcgacgga gatattgcgg cctcggccgg
     3841 cgccccttac gaaaaaggct ggcccggaaa cgaacagctg cttcaaccgc tcaccctggc
     3901 cttcgtgatc gccaatattt cccagtacct gcgcgaccac tgggccgagg aatacccgaa
     3961 attgaacgcg atcatgaagc gcgcctacca gaacggcgcc tacccggtca agcgccaacc
     4021 cggcggactc ttcccgatgg ccggccgccg ggaactctat gccaatgcca cccgcatggc
     4081 cggcgatact accgatgtcc aggacctgac ccgggcggaa atcgaaggac ggcgccaggc
     4141 ctgggcgctg atgaaattct accgcgacaa cctgcccggt ttcgaagaga cctacctgcg
     4201 atataccgcc agccaggccg gtccgcgcga atcgcgccgg atcctgggcg attacgtcct
     4261 gaaccgggaa gacgttctgg aagggcgcga tttcccggac ggcgtagccc ggggcgctta
     4321 taccatcgac atccacaacc ccaaggacgg caccactcaa tacctgcgtc tgccgcccgg
     4381 caccagttac gccattccct atcgctgcct cgtcccccag cgggttgaaa atctgctggt
     4441 cgccgggcgc tgcatctcgg taaaccacga agcgctcggt tccatccgag tcatggccac
     4501 ctgcatggcc accggccagg cggccggcac cgccgccgcc ctggcggttc aggcgggaac
     4561 cactccgcgc cggctgccgg tcgtccggat tcaaaaacga ctgcaagccg acggcgcggt
     4621 gatctgaccg gaagcggtca ttagaaaaca gggggtaaaa tgaaaagatt cgactccgcc
     4681 ggaatcctgg ccttcggccg ggaagaaacc ctgaccgtcc gcctgttgct ctccctgacc
     4741 ctggccggag tcaccggtct gctggccggc atccgggtcc ccctcccctt caccccgatt
     4801 ccgcttaccg ggcaggtcct gggcgtttac ctggccggcc tgctcctgga cgcccctttc
     4861 gccggccttt ccctcctttt ctacctggtc ctgggcctgg ccggcctgcc ctggttcgcc
     4921 ggctggcagg gattgaacta tgccggattc ctggcctatc ccagcgccgg gtacctgctc
     4981 ggcttcatac cggccgcctt cctgatcggg cgcctcctgg aagacccggc ccgccgttcc
     5041 ctggcccggc aggtcctggc ctactcggcc ggcaccgccg tcttctactt ctgcggcgcg
     5101 gcctggctgg cggccctgac cggggccgga ttcatccgga ccctcgccat gagcgtctat
     5161 cccttcgtca tctttgacct gctcaaggcc ctgatggtgg cctcattcgc ctcggcgctg
     5221 atcggacttc gccgctgaaa tgcggttacg cggccaccac ctgctctgct tgctgggttt
     5281 ccggggactc ggttacagtc cggaattcgt ggcccggatg caggaagtcc gttcggcttt
     5341 aaattcaaaa cgccggttct tcatccaggt cgtcaccgaa tctgacgaca tctgccgtgc
     5401 ctgcccgtac cactccaaca acgcctgccg caaaggaagc ctgtcggtcc gccgcaccgc
     5461 cgaaatggac cggcggacac tccaatacct gggactccag cccggccgcc gcatcagcgc
     5521 cgatcggttg ctgaaaataa ttccgcagcg gcttggcgat ttcgaccgac tggtgaaaat
     5581 ctgcggccgg tgcggctggc gtgaggtgtg caattattac ctcgaactgc ggtcccggtg
     5641 gcgccgttcg gccttgtgag ccggttcccg gtatggtaat atagataacc ttaattccgg
     5701 agaaaacatg ggcgattacg aaacgattct caagcggcgc accatccggc gcttccgccc
     5761 cgacccggtg ccgccggaac tgctggagaa actggccgag gccggccggc tggccccctc
     5821 gggcggcaat cttcagccct gcgaattcat tatcgtcgac cagccggacc gggtggccga
     5881 gctcttcccg gccctgcgct gggcggccta cgtggctccg gccgggaatc cgcccgaagg
     5941 ccaccggccc accgcctacg tggtggtact ggtcaaccag agcatcaagc gcgagggacc
     6001 ggtcgactcg gccgcggcgg tgatgtcgat gatcctgacc gcccaggagg ccggtgtctc
     6061 ctcctgctgg cttggttcca tcgaacggga aaaactggcc gaaatgctcg gcatcccggg
     6121 gaactaccac atcgactcgg tcctggcgct cggttacccg gaccagaaga gctgcgtcga
     6181 ggagtaccgg gactcggtaa agtactggct cgatgaccgg ggcgattttc acgttccgaa
     6241 gcgcggcctg aaagaaatac tgcaccgcaa cgctttcgga cgccgctgaa gcccggttgc
     6301 ttacgggcca ggcaggcccc ggcctaaagt ttcagattga aaaggagagg tgcgagagcg
     6361 gttgaatcgg cacgattgga aatcgtgtag gccgcaaggc ctcgagggtt cgaatccctc
     6421 cctctccgct aaaccccact cttaagggga ttcgatggga gcgaagtcag gcgacgcgac
     6481 tgattaagga gcgttgccag c
//
LOCUS       DAOQYN010000108         6456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_12365_length_6456_cov_4.306515, whole genome shotgun sequence.
ACCESSION   DAOQYN010000108 DAOQYN010000000
VERSION     DAOQYN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12365_length_6456_cov_4.306515"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PKN80_05365"
     CDS             complement(<1..532)
                     /locus_tag="PKN80_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ35478.1"
                     /translation="MKLNAYHLLRVVRFGWKNLYLHKLRSLLTVLGIVFGVASVIAML
                     AIGEGASFEAREKIKQLGSNNIIIRAIKLAGEPTSQQETRLGVYGLNPEDFRRIKTIP
                     TVEKLVPCRETDQDIWYYEKNVSGRVVGTVPDYAAVMNLNIVNGRFFNEFELEGGKPL
                     AVIGASVKKTLFLAENP"
     gene            complement(522..1202)
                     /locus_tag="PKN80_05370"
     CDS             complement(522..1202)
                     /locus_tag="PKN80_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ35479.1"
                     /translation="MAAEVIRLDGISKIYRIADVEVAALKNLSLTVREGEHISIVGPS
                     GSGKTTLLNLLGCLDRPSSGNYFLDGQEISKLDDDALSHIRSQKLGFVFQSYNLIPQL
                     TVLENIGLPLFYQYADESAIQAKALELARIVGLGNRLYHRPFELSGGQQQRVAIARAL
                     INDSVIVLADEPTGNLDSSTGREVMDLLLDLNHKGTTLLLITHDPKVAAFGQRTIHML
                     DGEIVDET"
     gene            complement(1211..3046)
                     /locus_tag="PKN80_05375"
     CDS             complement(1211..3046)
                     /locus_tag="PKN80_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNQ35480.1"
                     /translation="MKKLLWKAILKILKNRFVLAALLLIVAGGLAIHFTNSQKKIRQL
                     ITQEVKRQDLLISVIEGGNLTALRSQKVINNVPGQRNILEVVDEGTRITEEDVRNGKV
                     LVRLDSEDLKKSAEQRRIEVESSWAQYLDAQENLEIQKKQNESDIKQAELKVKFAQMD
                     LAKYLGDSLADQVIKDTRLDYGQLISSPQLGGESLNKKKEVENRIDLANEELSRARDK
                     VEWSNRLSQKGFITKTEFQADELALKQKNVALEQANLESRLFFNYDFPKQVEKLLSDY
                     QESLKSLERTRANCRSRLIQGEASLKSRRSTYLLSKDRLEENEIQLKECTIVATQPGF
                     VTYATSSRPWGNQSPIQPGTQVRQRQDLLTLPDFDSMGVEVKIHESAVEKVRVDQPAV
                     IKVDAFPDRAFTGKVIKISLMPDPTLKWLNPDINVYVAQISFDQSYDFLKPGMSAQVE
                     ILVEKLEKVIAVPLVALTFHGETPYCTVLKNRMLETRRVTLGKSNDEMVEIKSGLAAG
                     ETLVISPGQMTPQIKKQELSEKGRMETTAPAIQTGNQPGSGRSESGAGPASPAAGNQP
                     ETGASSAGREGGGEGASSGRRGRGSRSGASSESTPAQPIVRPQQP"
     gene            complement(3015..4580)
                     /locus_tag="PKN80_05380"
     CDS             complement(3015..4580)
                     /locus_tag="PKN80_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNQ35481.1"
                     /translation="MACLPLLLLLAGCSAGFYERSADREVEKILAEKKPVVEQTALPE
                     FKTPREQVETDTIILSLRDCLTQAASRNREYQSRKEDLYLGILDLTYQRYLYRLRYGA
                     SGDVVWNRDGRENFTSSLNLNLVKWLATGAELTLNLGQSFLKYLTGDRTKDFQTFASL
                     NILQPLLRGAGRTAALENLTQAERNAVYDIRDFLTYQRDFSVDVAERFFNLLLWKNNL
                     ENYQTNYRNLSNILARIEMLSEAGRIDPFQVDQARQNEYSAYQRWLSAYNDYQSSLDS
                     FRIFLGYAPGTRVVLAETELERLLEKGIPRESRGAEIFLDSALEGRLDLMTTFDKVAD
                     SGRQLKIARNNLRGKLDLSLEAGQSTETESNPTVDFRNPSYQAGLAFELPLNRISERN
                     AYRRAQINLARSNRSLQAKVDAIRLEVLDSYRNQEEAYQSYLIQKRSLELAEKRIEST
                     DLLLQAGRATTRDLLDAQESYLSSRNALAETVVRNLISQLQLLKNAEQIELDDNGVWQ
                     GELYEKITLESNP"
     gene            4863..4936
                     /locus_tag="PKN80_05385"
     tRNA            4863..4936
                     /locus_tag="PKN80_05385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4897..4899,aa:Arg,seq:gcg)
     gene            5038..5865
                     /locus_tag="PKN80_05390"
     CDS             5038..5865
                     /locus_tag="PKN80_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35482.1"
                     /translation="MLAGTASRLRAEVPASLVSDLNGRWSSLKSLQADLSVGFQMFGN
                     TLNTGGRFWQQERLFRCELTVPQELSPNRQLTGDASRIQILFDGRTLWQSMPIWNLAY
                     KFDLTQLDTRLAEEMTPASFQLPAISYSTSEKVRNRKNYYLLETRDVAGFSRGNQLAA
                     SLYPVLGQLPFKRIQVWVGRESLLPEIVEFYGSQSVPIFFFRLQNIKTGQALAPELFT
                     FRMPEGSKVIDMTSSLAQLLKGGAATDSGAKPSAGRSGENGAGIDKAKPDSGIENKN"
     gene            6187..6276
                     /locus_tag="PKN80_05395"
     tRNA            6187..6276
                     /locus_tag="PKN80_05395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6221..6223,aa:Ser,seq:cga)
     gene            complement(6255..>6456)
                     /locus_tag="PKN80_05400"
     CDS             complement(6255..>6456)
                     /locus_tag="PKN80_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35483.1"
                     /translation="MKQSEARFMAVKCALSSVKALYFTALFKSLRKSGVLRIRLVSPV
                     FMADRRDLAIFCPAKNGGEGGI"
BASE COUNT         1123 a         1798 c         2100 g         1435 t
ORIGIN      
        1 ccgggttctc ggccaggaag agcgtctttt tgaccgaggc gccgatgacc gcaagcggct
       61 tgccgccttc cagttcgaat tcgttgaaaa aacggccatt gacaatgttg agattcatca
      121 ccgccgcgta gtcgggcacg gtgccgacca cccggccgga cacgtttttt tcgtagtacc
      181 agatatcctg gtcggtctcc cggcagggga cgagtttttc gacggtcgga atggttttga
      241 tccgcctgaa gtcctccggg ttgagcccgt aaacgcccag ccgggtttcc tgctgggagg
      301 taggttcgcc ggcgagcttg atggcccgga tgatgatatt gttgctgccc agctgcttta
      361 tcttctcgcg ggcctcgaaa ctggctcctt cgccgatggc cagcatggcg atcaccgagg
      421 cgaccccgaa gacgatgccc agtacggtca gcagcgaacg cagtttgtgg aggtagaggt
      481 tcttccaccc gaagcggacg acccggagaa gatggtaggc gttaagtttc atcgacgatc
      541 tccccgtcca gcatgtggat ggtccgctgg ccgaaggccg cgaccttggg gtcgtgggtg
      601 atgagtagta gggtggtgcc cttgtggttc aggtccagca ggaggtccat gacttcccgg
      661 ccggtgctgc tgtccaggtt gccggtcggt tcgtcggcca gcacgatgac cgaatcgttg
      721 atcagggccc gggcgatggc cacccgctgc tgctgtccgc cggagagttc gaagggacgg
      781 tggtagagcc ggttgcccag cccgacgatc cgggccagtt ccagggcctt ggcctggatg
      841 gccgattcgt cggcgtactg gtagaagagg ggcaggccga tgttctccag cacggttaac
      901 tggggaatca ggttgtagga ttggaagacg aagcccagtt tctggcttcg gatatgggag
      961 agggcgtcat cgtcgagttt cgagatttct tggccgtcca ggaagtagtt gcccgaagag
     1021 ggccggtcca ggcagcccag cagattgagc agggtggttt tgccgctgcc cgaggggccg
     1081 acgatggaaa tatgctcccc ctcgcgtacc gtgagagaga ggtttttgag tgcggccacc
     1141 tccacgtcgg cgatccggta aatcttgctt attccgtcca ggcggatgac ttcggctgcc
     1201 atggcgtgct tcagggttgt tgaggcctta cgatcggttg cgcgggcgtt gattcggaag
     1261 acgcgcccga acgggagccg cgtccccgac ggccgctgga ggcgccttct ccgccgcctt
     1321 cccggccggc ggacgaggct ccggtttccg gctggttgcc ggcggccggt gaagccgggc
     1381 cggccccgga ttccgaacgg ccggatcccg gctgattgcc ggtctgaatg gccggggcgg
     1441 tggtctccat ccgccctttt tcagagagtt cctgcttctt gatctgcggg gtcatctgtc
     1501 ccggcgagat taccagcgtc tcgcccgcgg ccaggccgga ctttatctct accatctcat
     1561 cgttactttt gcccagcgtt acccggcgcg tttcgagcat gcggttcttc aggacggtgc
     1621 agtaaggggt ttcgccgtgg aaggtcaggg ccaccagcgg caccgcgatg accttctcaa
     1681 gtttttcaac cagaatctcc acctgggccg acatgcccgg cttgagaaaa tcataactct
     1741 ggtcgaagga gatctgggcg acgtaaacgt tgatgtccgg gttgagccac ttgagcgtcg
     1801 ggtcgggcat cagcgagatc ttgatgactt tgccggtgaa ggcccggtcc gggaaggcat
     1861 ccaccttgat gaccgccggc tggtcgaccc gcaccttctc cacggccgat tcgtggatct
     1921 tgacctccac gcccatgctg tcgaaatcgg gcagggtcaa caggtcctgg cgctggcgca
     1981 cctgggtgcc gggctggatc gggctctggt ttccccaggg gcgcgaactg gtggcgtagg
     2041 taacgaaacc gggctgggtg gccacgatcg tacactcctt cagttggatt tcattctcct
     2101 ccagccggtc cttgctgagc aggtaggtgg accggcggct tttgaggctg gcctcgccct
     2161 ggatcaggcg ggatcggcag ttggccctgg tccgctccag ggatttgagc gactcctggt
     2221 aatcggaaag gagcttctcg acttgcttgg ggaaatcgta attgaagaag agccttgatt
     2281 ccagattggc ctgttccagg gcgacgttct tttgtttcag ggccagttca tcggcctgga
     2341 attcggtctt ggtgatgaag cccttctgag agagccggtt gctccactcc accttgtccc
     2401 gggcccggga gagctcctcg ttggccaggt cgatccggtt ttcgacctcc ttcttcttgt
     2461 tcagtgactc gccgcccagc tggggactgg aaatgagctg tccataatcg agccgggtgt
     2521 cttttatcac ctggtcggcc agtgaatcac ccaggtactt ggccaggtcc atctgggcga
     2581 atttcacctt gagttcggcc tgcttgatgt cgctttcatt ctgcttcttc tgtatctcca
     2641 ggttctcctg ggcgtccagg tactgggccc agctgctttc gacttcgatc cggcgctgct
     2701 cggcgctctt cttcaggtct tcggagtcga gccggacgag caccttgccg ttgcggacgt
     2761 cctcctcggt gatccgggtg ccttcatcca ccacctccag gatgttgcgc tggccaggca
     2821 cgttgttgat cactttctgg gagcgcaggg cggtcaggtt gccgccctca atgaccgaga
     2881 tgagcaggtc ctgccgcttc acctcctggg tgatcagctg gcggatcttc ttctgactgt
     2941 tggtgaaatg aatggccagc ccgcccgcga caatcaaaag cagagcggcc agcacgaacc
     3001 ggtttttcag gattttaagg attgctttcc agagtaattt tttcatagag ttctccttgc
     3061 cagactccgt tgtcgtcgag ctcgatctgt tcggcattct tcaggagctg gagctgggaa
     3121 ataaggttgc ggaccaccgt ttcggccagc gcgttgcgcg agctgagata ggattcctgg
     3181 gcgtcgagca ggtcgcgggt ggtcgcccgg ccggcctgca ggagcaggtc ggtgctttcg
     3241 atgcgtttct cggccagctc caggcttctt ttctggatca ggtaactctg gtaggcctcc
     3301 tcctggttcc ggtaagagtc gaggacttcg agccggatgg cgtccacctt cgcctgcagg
     3361 ctgcggttgc tgcgggccag gttgatctgg gcgcgccggt aggcgttgcg ttccgagatc
     3421 cggttcagcg gcagttcgaa ggccaggccg gcctggtagg atgggttccg gaagtccacg
     3481 gtcgggttgg attccgtttc ggtgctctgg ccggcctcca gtgaaaggtc gagcttgccg
     3541 cgcaggttgt tccgggcgat cttcagctgg cggccggaat cggccacctt gtcgaaggtg
     3601 gtcataaggt cgagccgccc ttccagggcc gagtccagga aaatttccgc gccgcgcgac
     3661 tcgcggggga tgcccttctc caggagcctt tccagttcgg tctcggccag caccacccgg
     3721 gtgccgggcg cgtagcccag gaagattcgg aaactgtcca gggacgactg gtaatcgttg
     3781 taggcgctca accagcgctg gtaggcgctg tactcgttct ggcgggcctg gtccacctgg
     3841 aagggatcaa tgcggccggc ctccgagagc atctcgatgc gggccaggat gttgctcagg
     3901 ttgcggtagt tggtctggta gttttccagg ttattcttcc acagcagcag gttgaagaag
     3961 cgttcggcca cgtccaccga aaagtcgcgc tggtaggtga gaaaatcgcg gatatcgtaa
     4021 acggcgttcc gctcggcctg ggtgaggttt tccagggcgg cggtccggcc ggcccccctg
     4081 agaagcggct ggaggatgtt cagcgaggcg aaggtctgga aatccttggt ccgatcgccg
     4141 gtcaggtact tcaggaagct ctggccgaga ttgagggtca attcggcgcc ggtggccagc
     4201 cacttgacca ggttgagatt gagcgaactt gtgaagtttt cccggccgtc ccggttccag
     4261 acgacgtcac cgctcgcccc gtagcggagg cggtaaaggt agcgctggta ggtgagatcc
     4321 aggatgccca ggtaaaggtc ttccttccgg gactggtatt cgcggttgcg gctggcggcc
     4381 tgggtcaggc aatcacgcag ggagaggata atcgtgtcgg tttcgacctg ctcccggggc
     4441 gttttgaact ccggcagggc ggtctgctcg accacgggtt ttttttcagc caggatcttc
     4501 tcgacctccc ggtcggccga tcgctcgtag aaaccggcac tgcagccggc cagcaggaga
     4561 agcagcggta aacaagccaa aaatcctttt cgcattttgg gtttgggagt ttcggaaaaa
     4621 cgggcggcgt caacaggttt taacgatgtc tttccaatgg attttagtgg gaaaattccc
     4681 gggattcgtt tatggcgccg gtttcctgtc ggtcaagttt tgtctaataa taatagcaca
     4741 gaaagcccct cctggaaaag ggcgcccgaa gccgggactg gtaaccgccg ggaggcgacg
     4801 ccggactccg ggccgcccgg cggctgaaaa ggagtggcct tttatggtat cataatataa
     4861 ttgcgcccat agctcaattg gatagagcgt cagattgcgg atctgaaggc tggtggttcg
     4921 agtccacttg ggcgcgcttt ttttgctggg ggtttttaat ggcggtccgg accgcgggag
     4981 acaacaatgc gattgcttac gttaaaacgg attgggattt cgctgacggc gctgttattg
     5041 ctggcgggaa cggccagccg gcttcgggcc gaggtgcccg cgtccctggt ttcggacctc
     5101 aacggccgat ggagctcgct taaaagcctc caggccgacc tcagcgtcgg cttccagatg
     5161 ttcgggaaca cccttaatac cgggggacgt ttctggcagc aggagagatt attccggtgc
     5221 gagttgacgg tgccccagga actgtcgccg aaccgccagt tgaccgggga cgcctcccgg
     5281 atacagatac tttttgacgg gcggaccctc tggcagtcga tgccgatctg gaacctggcc
     5341 tacaagttcg acctgaccca gctggatacc cggctggcgg aggagatgac cccggccagt
     5401 ttccagttgc cggcgatttc ctactcaacc agcgaaaagg ttcggaatcg gaagaattat
     5461 tacctcctgg agacgcgcga tgtggccgga ttctcccggg gcaaccagct ggcggccagt
     5521 ctctacccgg tcctgggcca gttgccgttc aagcggattc aggtctgggt gggccgggaa
     5581 tcgctgctgc cggagatcgt cgagttttac ggttcccagt cggtgccgat cttcttcttc
     5641 cgccttcaga acatcaagac cggccaggcc ctcgctccgg aactcttcac cttccggatg
     5701 ccggagggca gcaaggtcat tgacatgact tcttcgctgg cccagctgct gaagggtggc
     5761 gcggctaccg attccggggc gaaaccgtcc gccgggcgat ccggggagaa cggcgccgga
     5821 attgataaag cgaagccgga ttccgggatc gaaaacaaga attgagcctg gttcagagaa
     5881 ttgagattta gttgatggaa ctggagaggt gcctgagcgg ctgaaagggc cggtctcgaa
     5941 aaccggtagg cggcttgccg cctcgggggt tcaagtcccc cccggtgcgg cttcctgaca
     6001 aggggggggg acttgatggg agcgaagtca ggcaaggcga ccgaataggg agcgttgcca
     6061 gcgggtggcc gacccgaggc gcagtgggag ccgagggcgc caaatccctc cctccttcag
     6121 gttttcagac ggaatttgga tctgttttga gcttggtttc gagagttgga tttagttgat
     6181 ggaattggag aggtgcctga gcggctgaaa gggccggtct cgaaaaccgg taggcggctt
     6241 gccgcctcgg gggttcaaat ccctccctct ccgccatttt tcgcagggca aaaaatggcg
     6301 agatctcgcc tgtccgccat aaaaactggc gagacgaggc ggatccgcaa aaccccactc
     6361 ttacgcaggg atttgaagag agccgtgaag tagagcgctt tgaccgagct cagcgcacac
     6421 ttcacggcca tgaagcgtgc ttcgctctgc ttcatg
//
LOCUS       DAOQYN010000109         6241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_12872_length_6241_cov_4.208050, whole genome shotgun sequence.
ACCESSION   DAOQYN010000109 DAOQYN010000000
VERSION     DAOQYN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12872_length_6241_cov_4.208050"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKN80_05405"
     CDS             <1..261
                     /locus_tag="PKN80_05405"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNQ35484.1"
                     /translation="GKKPEEIAFIVPHQANLRIIQAVVKSLGVSMDKVYLNIERYGNM
                     SAASVAVGLDEVITRGLVHSGDLVLMFTFGAGLTWAATVLRV"
     gene            392..1342
                     /locus_tag="PKN80_05410"
     CDS             392..1342
                     /locus_tag="PKN80_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNQ35485.1"
                     /translation="MKNSVAKTLLAACFLILIATVSQAQQEYIVQPGENLYRIASRFE
                     TSVDTLKRLNGLASNLIHPGDRLIIDRVENPETEDSLSSEVGYVYQTQKTWHRVRSRE
                     TLAAIARRYGLTVTRLKSLNHLSGNLIHPGDRLLVEEKQVRVPAVSEPVLEVARNVPA
                     ADLAPEKPSGWLDDLNLNPTVSCLLENALEFLGAPYRLGGLSRLGVDCSGLVKKAFDS
                     VGIEVPRTARTQYNIGKPVSLEEILPGDLLFFATRAARYPTHVAIYLGNGLVLHASSG
                     SRRVVVDNFENSRYLQSRFIGARRILSGGEEVEAELVPRE"
     gene            1406..2398
                     /gene="obgE"
                     /locus_tag="PKN80_05415"
     CDS             1406..2398
                     /gene="obgE"
                     /locus_tag="PKN80_05415"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNQ35486.1"
                     /translation="MFVDYVEILAASGKGGDGLVAFRREKYIPKGGPSGGDGGHGGSV
                     IIRSSPGVQTLIDFRHRHHFRAENGQPGGANRRTGRSAEDLFILVPVGTVVREKGGAV
                     LADLNRPGAEFPVARGGQGGWGNARFATSVRQAPRFARPGKPGEERQLVLELKLIADV
                     GLVGYPNSGKSTLLSVISNSRPRIAPYPFTTLEPCLGLVKVPDGSSFVAADLPGLIEG
                     AHQGRGLGDRFLRHIERTRFLLHLVDLSEAEPLERYRKIRRELVAYGCGLETKPELVV
                     GTKLDLPEARRRAPAFKRELAREGRESLVICAVSGQGLESLLRSVAGRLRELGR"
     gene            complement(2411..3655)
                     /locus_tag="PKN80_05420"
     CDS             complement(2411..3655)
                     /locus_tag="PKN80_05420"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNQ35487.1"
                     /translation="MNPVVVQKYGGSSVATTEKIARIAEKIKRRVDQGARLAVVVSAV
                     GDTTDYLLELAEQIGSQERGPELDLLLSTGEIVSASLMAMALNRIGVPARAFTGREAG
                     IITDTTHTKARIRRLQTGRIKAVLREGSVAVITGFQGVAAGDRVTTLGRGGSDLSAVA
                     LAKGLKAGCCEIYTDVPGIFTADPRVEPESRLIPRLSYEEALEMAAAGAAVMQARAVE
                     FGQKYRVPILVKSTFEEGNGTMITRVNHRRRSGSNLEEPLVSAVTGSGQEAKVSIFGV
                     PDRPGLAARIFSSVAAANINVDMIIQNVSKDGRTDLSFTIPADELKRAREIASQVAAE
                     IGAAGTAFDSDIGKVAIIGIGMQSHPGVAARMFSALGREGINIEMISTSEIKISCVVR
                     KSDLVRAVRVLHAEFGLSRKPA"
     gene            complement(3652..4410)
                     /gene="lgt"
                     /locus_tag="PKN80_05425"
     CDS             complement(3652..4410)
                     /gene="lgt"
                     /locus_tag="PKN80_05425"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNQ35488.1"
                     /translation="MHPILLKLGAFPIYTYGVMAALGVGLSAWLGIRRAVRSGLSEDL
                     VYNFVFITIISGLLGARLVHVAAEWVYYRNHLLEIFLIRRGGLAVQGALLAGMATANF
                     YLARLRLPARTVLDHFFVYLPLGQAIGRLGCFFNGCCYGMDTRLPWGLHFPGLSETVH
                     PTQLYYLLADLLLLAFLVRLGRNRTPGGTITAAYLVGFGLIRCLLDPLRGDLLPAFLG
                     LYATQWYGILFILIGLVVYLTRSRESEPGSGEAE"
     gene            complement(4418..4882)
                     /locus_tag="PKN80_05430"
     CDS             complement(4418..4882)
                     /locus_tag="PKN80_05430"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNQ35489.1"
                     /translation="MPRRVFLAPLTLAASAYLLDQGSKYFLSKHLHRPLFLIPRYVGL
                     VPHHNQGIIFGLCRIPFLALFSTLFALLVAWLTLTFLKEYPGSRSLRIGIGLIWGGVA
                     GNLTDRLLSGAVFDFLLLFPIPLFNLADLFLTAGALLILFPVFFVERQRIRV"
     gene            complement(4886..5248)
                     /locus_tag="PKN80_05435"
     CDS             complement(4886..5248)
                     /locus_tag="PKN80_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNQ35490.1"
                     /translation="MEKKTLERFRKALEKERDRLLKSLEGLEKAGSLNTERDTEKSGI
                     TTHPADQGTDNFERELQLGLVTEENTSLQEVEEALARIESKTYGRCESCGCAIESKRL
                     MVLPHARYCLGCRTKMES"
     gene            complement(5286..5714)
                     /locus_tag="PKN80_05440"
     CDS             complement(5286..5714)
                     /locus_tag="PKN80_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNQ35491.1"
                     /translation="MKPEFREFEHTADRGLELAATSLAGLFRAALAGLFFLYTGRPLP
                     ESPGEATPFRIRLAAGNAEELLVKTLNEAIFRTERAGHPFRPVELRLRKRPAGGLSLT
                     LGLTNVIIFPIIAEIKSATHHQLKIEKERGVWRCRVIFDV"
     gene            complement(5750..>6241)
                     /locus_tag="PKN80_05445"
     CDS             complement(5750..>6241)
                     /locus_tag="PKN80_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35492.1"
                     /translation="QVLDTVSIDAFAFWEDMAYRHGPLISPVLARRYMLPRYRRVVDF
                     LRGRGVEFIGLDSDGRIDGLIPVWLEAGLNFLFPFEVQSGMDVNAVRREYGRELLIWG
                     GVDKRALAADESRIEAELTRIRPLIEAGGYIPFTDHSLPPDVPFENYRYYLERLSEIC
                     GRG"
BASE COUNT         1213 a         1968 c         1952 g         1108 t
ORIGIN      
        1 ggcaaaaaac cggaggagat cgcctttatc gttccgcacc aggccaacct gcgcatcatc
       61 caggcggtgg tgaaatcact cggcgtttcg atggacaagg tctaccttaa catcgaacgc
      121 tacggaaaca tgtcggccgc ctcggtggcg gtcggcctgg acgaggtgat cacccgggga
      181 ctggttcaca gcggcgacct ggtcctgatg ttcaccttcg gcgccggatt gacctgggcg
      241 gccacggtcc tgcgcgtttg aagaaacggc cgggcttgac aattatccgg ttcctcaagt
      301 ataataagac ctaaaatcaa cttggcgtat ccaacacacc aagagcaggt cgaaagctgg
      361 ttctcggcct cggttacttg aagaggtgcc attgaagaac tcagtcgcaa agaccctcct
      421 ggcggcgtgt ttcctcatcc tgattgccac cgtttcccaa gcccagcagg aatacatcgt
      481 ccaacccgga gagaatcttt accgcatcgc cagccgtttt gagaccagcg tcgataccct
      541 gaagcgtctt aacgggctgg cgagcaatct tattcacccg ggtgaccgcc tgatcatcga
      601 ccgggtcgag aatccggaga ccgaggatag cctttcctcg gaggttggtt atgtctatca
      661 gacccagaag acctggcacc gggtgcgttc cagagaaacc ctggccgcca tcgcccgccg
      721 ctacggcctt accgtcacca ggctgaaaag cctcaaccac ctttcgggca acctcatcca
      781 tccgggagac cggctcttgg tcgaggagaa gcaggtgcgg gtgccggcgg taagcgaacc
      841 ggtcctggag gtggcccgca atgtcccggc cgccgacctg gcgccggaaa aaccatccgg
      901 gtggctggac gacctcaacc tcaatccaac cgtttcctgc ctgctggaaa acgccctgga
      961 atttctcggc gcgccttatc gcctcggggg cttgagccgc ctgggggtcg attgttcggg
     1021 cctggtcaag aaggcctttg acagcgtggg tatcgaagtg ccccgtaccg ctcgcaccca
     1081 gtacaacata ggcaaaccgg tctccctgga agaaatcctg cccggcgacc tgctcttctt
     1141 cgccacccgg gcggcccgtt acccgaccca tgtcgccatt tacctgggca atggcctggt
     1201 cctgcacgcc agcagcggat cccggcgggt ggtggtggac aacttcgaaa attccagata
     1261 cctgcagagc cgtttcatcg gcgcccgccg catcctttcc ggaggcgaag aggtggaggc
     1321 ggaactggtg ccgagggaat gagcgcggcc gtattctccc ccatcccgga ttctctcccg
     1381 aaaacgcact accttacact ctgccatgtt tgttgactac gttgaaatat tggccgcttc
     1441 gggcaagggc ggcgacggcc tggttgcctt ccggcgcgaa aagtatatcc ctaagggggg
     1501 gccgtccggc ggagacggcg gccacggcgg atcggtcatc attcgctcca gcccgggcgt
     1561 tcagaccctg attgatttcc gtcaccgcca tcacttccgg gccgaaaacg gccagcccgg
     1621 cggagccaac cggcggaccg gccggtccgc ggaggacctc ttcatcctgg ttccggtggg
     1681 tacggtggtg cgcgaaaagg gcggggcggt cctggccgat ttgaaccggc ccggcgccga
     1741 gtttccggtc gcccggggcg ggcagggggg gtggggaaac gcccgcttcg ccacttcggt
     1801 gcgccaggcg ccgcgcttcg cccgccccgg caagccgggc gaagaacgcc agctggtgct
     1861 ggaactcaag ctgattgccg acgtggggct ggtcggctat cccaacagcg gtaaatcaac
     1921 cctgctgagc gtcatctcca acagccggcc gcggattgcc ccctatccgt ttaccaccct
     1981 ggaaccctgc ctgggcctgg tcaaggtgcc ggacgggagc agtttcgtgg ccgccgacct
     2041 gcccggcctg atcgagggcg cccaccaggg acgcggcctg ggcgaccgtt ttctgcgcca
     2101 tatcgaacgg acccggttcc tgctccatct ggttgacctt tccgaggccg aaccgctgga
     2161 gcgttaccgg aagatcaggc gggaactcgt tgcctacggg tgcggcctgg agacaaagcc
     2221 agagctggtg gtgggaacca agctggacct gccggaggcg cgccggcgcg ccccggcctt
     2281 caagcgggaa ctggcccggg aggggcggga aagtctcgtc atctgcgcgg tctccggcca
     2341 ggggctggaa tccctgctgc ggagcgtcgc cggccgcctc cgggaactcg gccgctgaaa
     2401 ccggctggtt tcaggcgggc ttccggctca gcccgaactc ggcgtgcagg acccggaccg
     2461 cccggaccag gtcggatttg cggaccacgc aggaaatctt gatctccgag gtgctgatca
     2521 tctcgatatt gatcccttcc cttcccaggg cgctgaacat ccgggcggcc accccgggat
     2581 gactctgcat gccgataccg ataatcgcga ccttgccgat gtcggagtcg aaggcggttc
     2641 cggccgcccc gatttcagcg gcgacctggc tggcaatctc acgggctcgt ttcaattcgt
     2701 cggccgggat ggtgaaggaa aggtcggtcc ggccatcctt gctgacgttc tggatgatca
     2761 tgtccacgtt gatgttggcg gcggccaccg aggagaagat acgggcggcc aaccccggcc
     2821 ggtccggcac tccgaagatt gagaccttgg cctcctggcc gcttccggtc accgccgaga
     2881 ccaggggctc ctcgagattg ctcccgctgc gccgccgatg gttgacacgg gtaatcatgg
     2941 ttccgttccc ctcctcaaag gttgacttga ccagtatcgg cacccggtac ttctggccga
     3001 attcgaccgc gcgcgcctgc atcaccgccg ccccggccgc ggccatctcg agcgcctcct
     3061 cgtaggaaag cctcggtatg agacggctct ccggttcaac acgggggtcg gcggtgaaga
     3121 tgccgggcac atcggtgtaa atctcgcagc agcccgcctt cagtcctttg gccagggcca
     3181 cggccgaaag gtcgctgccg ccccggccca gggtggttac ccgatccccg gcggccacgc
     3241 cctgaaatcc ggtgatgacc gccacgcttc cttcccgcag aaccgccttg atccgcccgg
     3301 tctggagccg gcggatgcgg gccttggtat gggtggtgtc ggttatgatg ccggcctccc
     3361 gcccggtaaa ggcccgggcc ggaaccccga tccggttcaa ggccatggcc atcaggctcg
     3421 ccgaaacgat ctccccggtg gaaagaagca ggtccagctc cggaccccgc tcctggctgc
     3481 cgatctgttc ggccagttcc agcaggtaat cggtggtatc gcccacggcc gagaccacca
     3541 ccgccagccg ggccccctgg tcaacccggc gcttgatttt ttcggcgatc cgggcaatct
     3601 tttcggtggt tgccaccgag gatcccccgt acttctggac cacgaccgga ttcattccgc
     3661 ctccccggac cctggttcgg attcccggga ccgggtcagg taaacaacaa gtccgataag
     3721 aatgaagagt atcccgtacc actgggtggc atagagtccg agaaaagcgg gcagcaggtc
     3781 gccgcgcaaa gggtcgagca gacaccggat gagcccgaag ccgaccaggt aggcggcggt
     3841 gatggtgccg cccggagtcc ggttgcggcc gagccggacc agaaaggcca gcaggagaag
     3901 gtcggccagc aggtaataga gctgggtcgg atgcaccgtt tcggacaggc cggggaaatg
     3961 gagtccccag ggaagccggg tgtccattcc atagcagcag ccgttgaaga agcagcccaa
     4021 ccggccgatg gcctgaccca gcggcaggta aacgaagaag tggtccagca ccgtccgggc
     4081 cggcaggcgc agccgggcca ggtaaaagtt agccgtggcc atcccggcca gcagcgcccc
     4141 ctgcacggcc aggccgcctc tccggatcag gaagatctcc agcaggtggt tgcggtagta
     4201 aacccattcg gcggcgacat gaaccagccg ggcgccgagc agtccgctga tgatggttat
     4261 aaaaacaaaa ttatagacca ggtcttcgga gagtccggat cggacggcgc gcctgatgcc
     4321 aagccaggcg ctcagaccca cgcccagggc ggccatcacc ccgtaggtgt agatcgggaa
     4381 agcgcccagt ttcagcagga tcggatgcat cgaaatatta taccctgatc cgctgccgtt
     4441 ccacaaagaa gaccgggaag agaatcagga gagcgccggc ggtcaaaaag agatcggcca
     4501 ggttgaaaag cggaatcgga aagagcagca ggaaatcaaa gacggctccg gaaagcagcc
     4561 gatcggtcag gttgccggcc accccgcccc agataaggcc gatgccgatt ctcagcgaac
     4621 gggaacccgg gtactccttc aggaaggtaa gcgttaacca ggcgaccagg agggcgaaga
     4681 gcgttgaaaa aagggccaga aacggtatcc ggcagaggcc gaagatgatg ccctggttat
     4741 ggtggggaac caggccgacg tacctgggaa tcaggaagag gggccgatgg aggtgtttgg
     4801 aaagaaaata cttgctgccc tgatcgagaa ggtaggctga ggccgccagc gtcaggggtg
     4861 ccagaaaaac acggcgaggc acaagtcagg actccatctt ggtccggcag ccaaggcagt
     4921 agcgggcgtg aggcaggacc atcaatcgct tcgattcgat cgcgcagccg catgactcgc
     4981 aacggccgta agttttgctt tcaatccggg ccagcgcctc ttccacctcc tgaagcgagg
     5041 tgttctcctc ggtcaccagc cccagctgga gctcccgttc gaaattgtcg gtaccctggt
     5101 cggccggatg ggtggtaata ccactctttt cagtgtcgcg ctcggtattc agggaacccg
     5161 ccttttccag gccttccagg cttttcaaaa gccggtcccg ttctttttca agcgctttcc
     5221 ggaatctttc aagcgttttc ttttccattc gagctcccgc gcgttgtttc tcgagatgaa
     5281 aaccactaca cgtcgaaaat cacccggcac cgccagacac cacgttcttt ctctattttc
     5341 aactgatgat gcgtggcact tttgatctcg gcaattatgg ggaaaattat aacattcgta
     5401 aggcctaatg tcaaagacag gccgccggcc ggacgcttgc gcaggcgcag ttcgaccggg
     5461 cggaaaggat gtccggcccg ttcggttcgg aaaatcgcct cgttgagcgt tttgaccagc
     5521 agttcctcgg cattcccggc ggccagccgg atacggaacg gcgtcgcttc ccccgggctt
     5581 tccggaagcg gccggccggt gtaaagaaaa aagagtccgg ccagggcggc ccggaagagg
     5641 cccgccaggc tggtggcggc caattccagc ccgcggtcgg cggtatgctc gaactcccgg
     5701 aattccggtt tcatttctgg cggcctccgg tcgggcggtt ccgcttccgc taaccccgcc
     5761 cgcagatttc gctcaaacgt tccaggtaat aacggtaatt ttcaaaagga acgtcgggcg
     5821 gcaggctgtg atcggtgaac gggatgtagc cgccggcctc gatcagcggc ctgatccgcg
     5881 tcaactcggc ctcgatccgg gattcgtccg cggccagggc gcgtttgtcc accccgcccc
     5941 agataagcag ttcccggccg tattcgcggc gcaccgcgtt gacgtccatc ccggactgca
     6001 cctcaaaggg aaagaggaag ttaagtccgg cctcgagcca gaccgggatg agtccgtcaa
     6061 tccggccgtc gctgtcgagg ccgatgaact caaccccgcg cccccgcagg aaatcaacca
     6121 cccgccggta gcggggcagc atgtatcggc gggccaggac gggggagatc agcggcccgt
     6181 ggcggtaggc catatcctcc cagaaggcga aggcgtcgat cgaaacggtg tccaggacct
     6241 g
//
LOCUS       DAOQYN010000110         6192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_12987_length_6192_cov_4.758514, whole genome shotgun sequence.
ACCESSION   DAOQYN010000110 DAOQYN010000000
VERSION     DAOQYN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12987_length_6192_cov_4.758514"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PKN80_05450"
     CDS             complement(<1..494)
                     /locus_tag="PKN80_05450"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35493.1"
                     /translation="METKDTLDASRSKDLDACRLAVKNNPRDYRAHLDFGTACFNLGH
                     YNRAVRAFRCAIILAPDNYETQAALGQAYFYLSRYREALPFFKRVVAIKPDFAAAHAS
                     LGRIYTFLDRPAEAVESLKLAIRYDSTDTHTHTLLGNVYIRFDMNREAVAAFQEALDA
                     PPRA"
     gene            complement(657..1313)
                     /locus_tag="PKN80_05455"
     CDS             complement(657..1313)
                     /locus_tag="PKN80_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNQ35494.1"
                     /translation="MNRKNNLSLDTWRVFHIMAEFVDGFEAFAGVGKAISIFGSARVK
                     AHDPYYQKAEKLARLLAEKGYAIITGGGPGIMEAANKGASDAGGQSFGLNIELPMEQK
                     PNPFIKTMLSFRYFFVRKYMFIKYAAGFVFFPGGYGTTDELTEVLCLIQTRRSPRVPL
                     VLVGRDYWKGLLEWIEKTLVAGNYITAQDNDMLVLVEEPEEALEFILNFQAKPNGDQG
                     "
     gene            1433..3793
                     /locus_tag="PKN80_05460"
     CDS             1433..3793
                     /locus_tag="PKN80_05460"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HNQ35495.1"
                     /translation="MSSLIWAAGPAGDWQEGYPIGNGRLAGLVLGGVGSERVLLNHEW
                     LWRGRNRFKDNEPKEPARLAEIRRLFFEGKVLEAGDRANDYLGGAGGTARKTRGLNRV
                     DPYQPLGDLRLDFDGGEAAEYRRQLDLAAGLVTVCGRTGAGEVRREFLAHACHPVIAV
                     RISGAPAVGFRLRLERSADPDCRLEAWTEPAACGFRGRFSEGLSFAVEARLHLPGGGR
                     CRPLPEGGLEVEAAPEAVILLAAAVSPEGGDPAPECRKGFEGVPAGWPELLRTHREEH
                     GRRYGRVGLELAGRDFSERPTPERLRFLANGHEDNDLYALFFNYGRYLLIASSWGAEL
                     PANLQGKWNPLLAPPWECDYHFDINIQMNYWLAEAGNLSECTAPLFDLVDRFRPHGRQ
                     AARQLYGCGGVWLPIQTDCWGRATPESYGHDVWVGAAAWIAQHFWWHYQYTLDRKFLR
                     ERAYPFLKEAAAFFEDYLAADPQGRLVPVPSQSPENQFEGGSWPVSLGAAATMDLQLI
                     TELLNECLAASRILGVDGAKRARWRAILKRLPPPKIGRWGQLQEWLEDYNELQPGHRH
                     ISHLFGLYPAALITPEDTPELAAAARRTLERRLASGGGYCGWSSALVAACFSRLGEGE
                     RALQALTVLLSKFVTPSLLGLHPPHYFQIDANLGGAAAVAEMLLQSHGGRLRVLPACP
                     TAWWTGRVRGLVGRGGFTVDIEWREGRLEGLRVHSRFGGTCRLRLPRPARIEVRCGKR
                     LVRRFRKAALEAAWKTAAGRDYELKITEAEIEEGSQFGRDLRYHHG"
     gene            3744..4871
                     /locus_tag="PKN80_05465"
     CDS             3744..4871
                     /locus_tag="PKN80_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35496.1"
                     /translation="MKKAANSGGIFDTTTVDSFPGKPGWAAARRRWLAYWEGSEVDRP
                     LLDVRAPVDSGRPPLEAPANLEDRYFDPDYVGRKWLRTVQGTYWAGESVPTDSILMGC
                     YALGCGPKVVFAPNTVWHPVIESGPEAPLAWSPGEGDPWRRKLDRVILHLLELGRNRF
                     LVGEHSQVPPNDLLMLLRGTEPFLMELVEAPERWQRRLDEVMPVWLELLNHFRGLIAG
                     YQKGCFWGWPGLWHPDYVLLTQSDMSALISPALFEKWVLREMDWMGERFNTLWYHLDG
                     PRAVVHLPALLSRPYLKVIQFVPGAGVAPNGPAYLELYRRVQAAGRGLDLHCPPAEAE
                     FLVRRLRPERLLLRVVTESPEAADEFVEKARRWCGSHLGRD"
     gene            4926..>6192
                     /gene="serS"
                     /locus_tag="PKN80_05470"
     CDS             4926..>6192
                     /gene="serS"
                     /locus_tag="PKN80_05470"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNQ35497.1"
                     /translation="MLDLKFIRENRDLVERAARQKNARVDLDAILSLDEERRRLLGEV
                     DALRHDRNRASEEVNRLKKAGQDASELINRVRGDAERVKELEGRLREVEAKLFELASY
                     LPNIPAPDVPEGNPPDGNVVVRSWGEAPRFDFKPLPHYELAERLGLIDFKAAGRMTGS
                     FFALYTGMGARLERALINFMLDRHRAEGYREIAPPLMVNRRSMFNTGQLPKLEADMYR
                     VESEDFFLIPTAEVPVTNLHQDAILSEEQLPLCYTAFTPCFRLEAGAYGKDTRGLMRL
                     HQFDKVEMVKFCRPEDSGAEHEKLTADAEAVLQALELPYRVVLLCTGDLSFAGSKCYD
                     LEAWAPGVDRFLEVSSCSNFEDFQARRCNTRYRTRTGEVRLVHTLNGSGVALARTVAC
                     LLETHQRQDGSVILPAALRPYLDNLEAITP"
BASE COUNT          950 a         2018 c         2172 g         1052 t
ORIGIN      
        1 ttcgcccgcg gcggggcgtc gagcgcctcc tggaaggccg cgaccgcctc ccggttcata
       61 tcgaaacgga tgtagacgtt gccgagcagg gtatgggtgt gggtgtctgt cgaatcgtat
      121 ctgatcgcca gtttcaaaga ttcgactgcc tccgctggac gatcgagaaa ggtataaatt
      181 ctaccgagag aagcgtgggc cgcggcgaaa tcgggcttga tggcaacgac ccgtttgaag
      241 aatggaagcg cctcccggta acgggagaga taaaagtatg cctggccgag ggcggcctgc
      301 gtttcgtaat tatccggggc gaggataatc gcacaccgga aggccctgac cgcccggtta
      361 taatgaccaa ggttgaaaca ggcggtaccg aaatcaagat gcgcgcggta gtccctcggg
      421 ttgtttttga ccgccagtcg gcaggcgtcg agatcttttg accgggaagc gtcgagggta
      481 tctttcgttt ccattttgac ggtttttccc gttcgctatt atattcctgg gcaggtgtga
      541 tctgtcaatt aaggcggaat atttgtgggc aggcatgcgg gagtaagggc gggcgataac
      601 gccagaatac ggaaagggtg ggaagagaat tgaatgaata gccggcaggg tcagcttcag
      661 ccctggtcgc cgttgggttt ggcctggaag ttcaggatga actccagggc ctcctccggc
      721 tcctcgacca ggacgagcat gtcgttgtcc tgggcggtga tgtagttgcc ggccaccagg
      781 gtcttctcga tccactccag caggcccttc cagtagtcgc ggccgaccag gaccagcggc
      841 acccgggggc tgcgccgggt ctggatcagg cagaggacct cggtcagctc gtcggtggtg
      901 ccgtagccgc ccgggaagaa gacgaagccg gcggcgtact tgatgaacat gtacttccgg
      961 acgaagaagt agcggaagga gagcatggtc ttgatgaagg ggttcggctt ctgttccatc
     1021 ggcagctcga tgttgaggcc gaaggattgg ccgccggcgt cgctggcccc cttgttggcc
     1081 gcctccatga tgccggggcc gccgccggtg atgatggcgt agcccttctc ggcgagcagc
     1141 cgggccagct tctcggcctt ctggtagtaa gggtcgtgcg ccttgacccg ggccgagccg
     1201 aagatgctga tggcctttcc caccccggcg aaggcctcga agccgtcgac gaactcggcc
     1261 atgatgtgga agacccgcca ggtatcgagc gagaggttgt ttttccggtt catttttctc
     1321 ctggcttccg ccggttccgc atgttaaaat cttgccaaga ccgcatcaat tatggtctat
     1381 atatgtaata ttataacttg aaaggaccct ttttcacaag gagaaaaggc tgatgagttc
     1441 tttaatctgg gcggccgggc cggccggtga ttggcaggag ggctacccga tcggcaacgg
     1501 ccggctggcc ggcctggtgc tgggcggcgt cgggtcggag cgggtgcttc tcaaccacga
     1561 atggctctgg cgggggcgga accgcttcaa ggataacgag ccgaaggagc cggcccggct
     1621 ggccgaaatc cgccgcctct tcttcgaggg taaggtcctg gaggcgggcg accgggccaa
     1681 cgattacctg ggcggggccg gcggcaccgc ccgcaagacc cggggtttga accgggtcga
     1741 tccctaccag ccgctgggtg acctccggct cgatttcgac ggcggggagg cggccgagta
     1801 ccgccgccag cttgacctgg ccgccggcct ggtcacggtc tgcggccgga ccggcgccgg
     1861 ggaggtgcgg cgcgaattcc tggcccacgc ctgtcacccg gtcatcgccg tccggatcag
     1921 cggcgcgccg gccgttggtt tccggctccg gctggagcgg agcgccgatc ccgactgccg
     1981 gctggaggcc tggacggagc cggcggcctg tggcttccgg gggcgattca gcgaggggct
     2041 cagcttcgcg gtcgaggccc ggctccacct gcccggcggc ggccgctgcc ggccgctgcc
     2101 cgaaggcggc ctggaggtgg aggcggcgcc ggaggcggta atcctgctgg ccgcggccgt
     2161 ttcgcccgag ggcggcgacc cggcgccgga gtgccggaag ggcttcgaag gggtgccggc
     2221 cggctggccg gaacttttac ggacgcaccg ggaggagcat ggccggcgtt acggccgggt
     2281 cgggctggag ctggccggcc gcgacttttc ggagcggccg accccggaac ggctccgttt
     2341 cctggccaat ggccacgagg acaacgacct ctacgccctc tttttcaatt acggccgcta
     2401 cctgcttatc gccagctcct ggggcgccga gctgccggcc aacctgcagg gcaagtggaa
     2461 ccctctgctg gccccgccct gggagtgcga ctaccatttc gacatcaata tccagatgaa
     2521 ttactggctg gccgaggccg gcaacctctc cgagtgcacg gcgccgctct ttgacttggt
     2581 cgaccggttc cggccccacg gccgccaggc cgcccgacag ctctacggct gcggcggggt
     2641 ctggctgccg atccagaccg actgctgggg acgcgccacc ccggagtcct acggccacga
     2701 cgtctgggtg ggcgcggccg cctggatcgc ccagcatttc tggtggcatt accagtacac
     2761 gctggaccgg aagttcctgc gcgagcgcgc ctacccgttc ctcaaggagg cggccgcctt
     2821 cttcgaggat tacctggcgg ccgatccgca ggggcggctg gtgccggtgc cctcccagtc
     2881 gccggagaac cagtttgagg gcggcagctg gccggtttcg ctcggcgcgg cggccaccat
     2941 ggacctgcag ctgatcaccg agctgctgaa cgagtgcctg gccgccagcc gcatcctggg
     3001 cgtggacggc gcgaagcggg cccgctggcg cgcgatcctg aagcggctgc cgccgccgaa
     3061 gatcggccgc tgggggcagc tgcaggagtg gctggaggat tacaacgagc tccagccggg
     3121 ccaccggcac atctcgcacc tctttggcct ttacccggcc gcgctgatca ccccggagga
     3181 taccccggag ctggccgcgg ccgcccggcg cacgctggag cggcgcctgg cctccggcgg
     3241 cggctactgc ggctggtcga gcgccctggt ggccgcctgc ttcagccggc tgggcgaggg
     3301 ggagcgggcc ctgcaggcgt tgacggtcct gctttcgaag ttcgtcacgc cctccctgct
     3361 gggcctgcac ccgccccatt acttccagat tgacgccaac ctgggcggcg ccgcggccgt
     3421 cgccgagatg ctcctccagt cgcacggcgg ccggctgcgg gtgctgccgg cctgcccgac
     3481 ggcctggtgg acgggccggg tgcgcggcct ggtcggccgg ggcggcttca cggtggacat
     3541 cgagtggcgc gaggggcggc tggaagggct ccgggtccat tcccggttcg gcgggacctg
     3601 ccggctgcgc ctgccccggc cggcccggat cgaggtgcgt tgcgggaagc ggctggtgcg
     3661 gcgcttccgg aaggcggcgc tcgaggcggc ctggaagacg gcggccggcc gcgattacga
     3721 attgaagata acggaggcgg agattgaaga aggcagccaa ttcgggcggg atcttcgata
     3781 ccaccacggt tgattccttc cccggcaagc ccggctgggc ggcggcccgc cggcgctggt
     3841 tggcctactg ggaggggagc gaggttgacc ggcccctgct ggacgtccgg gccccggtgg
     3901 attccggccg gccgccgctg gaagcgccgg ccaacctcga ggaccgctac ttcgatcccg
     3961 attatgtcgg tcggaaatgg ctccggacgg tccagggcac ctactgggcc ggcgagtcgg
     4021 tcccgaccga cagcatcctg atgggctgtt acgcgctggg ctgcgggccg aaggtggtct
     4081 tcgccccgaa cacggtctgg cacccggtca tcgagtccgg tccggaggca cccctggcct
     4141 ggtcgcccgg cgagggcgac ccctggcgcc ggaagctgga ccgggtgatc ctccatctcc
     4201 tggagctggg gcgcaaccgc ttcctggtcg gcgagcacag ccaggtgccg cccaatgacc
     4261 tgctgatgct cctgcgcggg accgagccgt tcctgatgga gctggtcgag gcgccggagc
     4321 gctggcagcg gcgcctggac gaggtgatgc cggtctggct ggaactgctc aaccatttcc
     4381 ggggcctgat cgccggttac cagaaggggt gcttctgggg ctggccgggc ctctggcacc
     4441 cggactacgt gctgctcacc cagtcggaca tgtcggccct catctcgccg gccctcttcg
     4501 agaagtgggt cctgcgcgag atggactgga tgggcgagcg cttcaatacc ctctggtacc
     4561 acctggacgg cccccgcgcc gtcgtccacc tgccggccct gctctcccgc ccctacctca
     4621 aggtgatcca gttcgtgccc ggagccggcg tggcgcctaa cgggccggcc tacctggagc
     4681 tttaccgtcg ggtgcaggcg gccggccggg gcctggacct gcactgcccg ccggccgagg
     4741 cggaattcct ggtgcgccgg ctgcggccgg agcggctgct tttgagggtg gtgacggaga
     4801 gcccggaggc ggccgacgag ttcgtcgaga aggcccggcg ctggtgcgga agccacctcg
     4861 gccgggattg aatcaccgcc gcgcgcttga ccttattttc gcccatatcg taaaattgaa
     4921 gcaccatgct ggaccttaaa ttcatccgcg agaaccgcga cctggttgag cgggccgccc
     4981 gccagaagaa tgcccgggtc gacctggacg ccatcctgtc cctggacgag gagcggcgcc
     5041 gcctcctggg cgaggtggac gccctgcgcc acgaccgcaa ccgggccagc gaagaggtga
     5101 accggctcaa gaaggccggc caggacgcct cggagctgat caaccgggtc cggggcgacg
     5161 ccgagcgggt caaggagctc gagggccggt tgcgcgaggt ggaggcaaag ctcttcgagc
     5221 tggccagcta cctgccgaac atcccggccc cggacgtgcc ggaggggaac ccgccggacg
     5281 gcaacgtggt ggtgcgcagc tggggcgaag cgccccgttt cgatttcaag ccgctgcccc
     5341 attacgagct ggccgaacgg ctcggattga tcgacttcaa ggcggccggc cggatgaccg
     5401 gcagtttctt cgccctttac accgggatgg gcgcccggct cgagcgggcc ctgatcaact
     5461 tcatgcttga ccggcaccgg gccgaggggt accgggagat cgccccgccc ctgatggtca
     5521 accggcggtc catgttcaac accggccagc tgcccaagct ggaggccgac atgtaccggg
     5581 tcgagtcgga ggacttcttc ctcatcccga ccgccgaggt gccggtgacc aacctgcacc
     5641 aggatgccat cctctccgag gagcagctgc ccctctgtta caccgccttc acgccctgct
     5701 tccgcctgga ggcgggcgcc tacggcaagg acacccgcgg actgatgcgc ctgcaccagt
     5761 tcgacaaggt ggaaatggtc aagttctgcc gccccgagga ttccggcgcc gagcacgaga
     5821 agctgaccgc cgacgccgag gcggtgctcc aggcgctgga actgccctac cgggtggtgc
     5881 tgctctgcac cggcgacctc tccttcgccg ggtcaaagtg ttacgacctg gaggcctggg
     5941 cgcccggcgt cgaccggttc ctggaggtct cctcctgcag caacttcgag gacttccagg
     6001 cccgccgctg caatacccgt taccggaccc ggaccggcga ggtgcgcctg gtccacaccc
     6061 tcaacggttc cggcgtggcc ctggcccgga ccgtggcctg cctgctggag acccaccagc
     6121 gccaggacgg ctcggtgatc ctgccggcgg ccctccgccc ctacctggat aacctggaag
     6181 ccatcactcc ct
//
LOCUS       DAOQYN010000111         6109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_13199_length_6109_cov_4.380410, whole genome shotgun sequence.
ACCESSION   DAOQYN010000111 DAOQYN010000000
VERSION     DAOQYN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13199_length_6109_cov_4.380410"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKN80_05475"
     CDS             <1..199
                     /locus_tag="PKN80_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNQ35498.1"
                     /translation="LTPTSYQHREMSLFGKEAAPAGPAPRPTIEAPRPAESEAPRVGR
                     NDPCPCGSGKKYKKCCGRNAA"
     gene            307..1689
                     /gene="dnaA"
                     /locus_tag="PKN80_05480"
     CDS             307..1689
                     /gene="dnaA"
                     /locus_tag="PKN80_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNQ35499.1"
                     /translation="MGKPLMHNDLDQVNTVWNAALKLVKNKVLPSAFSLWIEPLKPVP
                     GRESELVVEAPDGFFADWVQKHYGEVLLAACQVINPDLKQVLVRARTETGPAEPGRPA
                     EVAPAAAPVRRPPGNGNINPLYTFDNFVIGDSNRLAHAAALSVAQSPGTAYNPLFIYG
                     PVGVGKTHLMQAIGNHARHQAGLQVAFLSSEKFLNEFIESLQNKSTAAFRNRFREVDL
                     LLIDDVQFLGGKEETQREFFHTFNTLYDFHKQVIVSSDRPPGEMKDIEKRLVSRFEWG
                     LVVDIQPPDLETRVAIIKNKARLKNLDLPQEVAFCIAEKVEGNVRLLEGALNKLLACA
                     SLYEKSVVDLEFSQSILNTLFEASAVRPRMEVGRVQEFIGRHYNLKVSDLKSGRRQQS
                     LVMPRQLAIYLARKLTGASLPQLGEAFGGKNHTTILYTCRKVEDKLGKDPDFKEEVAR
                     LEKKLKQETN"
     gene            1827..2930
                     /gene="dnaN"
                     /locus_tag="PKN80_05485"
     CDS             1827..2930
                     /gene="dnaN"
                     /locus_tag="PKN80_05485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNQ35500.1"
                     /translation="MELKAQKKELTAILGKVIGVLNPKPTLPKLSYVLMDAAREMLTV
                     TGTDLEISIRIVRPLNSTKEGKVFVPGRKLLDILREFPEAEIKLETEKETLLRLSQGR
                     INLSLVGLKAEEFPQLAKIKNPSQFKVGSEMLKRALASTKPIVVVDENRPHLSGVLLT
                     TEDGWLTAVATDTRRLARFKTKTNLASNIKFILPTKAAEIIRSVWEEETELDVNLGTN
                     QAIFSGRDITLTTQIIDGNFPNYASIIPAEADLRPATVPVEGFLAALRRLRQLVSAGK
                     NMVRLDVQPKIMRLSGEAAGVGEGSEDLEIDYQGEPITAYFNPDYLMDGLRPLEKETD
                     FFLGLNPNVEKPSLLRPTKSGDYLYLIMPMRPV"
     gene            2927..3244
                     /locus_tag="PKN80_05490"
     CDS             2927..3244
                     /locus_tag="PKN80_05490"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNQ35501.1"
                     /translation="MKPRAMEPIRAIVRRVLKSAGGSPREEKDYLAGWEEVVAPEIRR
                     HCRAWFRSGRLEVEADNPTTSHYLQLQKEKILAALRKRRLPLRDLVVRPRRSPRPPAA
                     GRS"
     gene            3256..4122
                     /gene="dapA"
                     /locus_tag="PKN80_05495"
     CDS             3256..4122
                     /gene="dapA"
                     /locus_tag="PKN80_05495"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNQ35502.1"
                     /translation="MFQGSTVALVTPFRNGRIDEKKFQDLVEWQIGAGTDALLPCGST
                     GESATLSMEEHQRLIELTLEVSAGRRKVIAGTGSNNTAEALHLTQHAKKVGADAALII
                     TPYYNKPTPAGLLLHFSTIARAVDIPIILYNVPSRTGVSLTPGTVAELARIPNIVAIK
                     EASGSLDQVSSILDRSDITVLSGDDSLTLPIMSVGGRGVISVAANVIPAGVAALTRAG
                     LAGDLAGAQAEHRRWFPLFQALFFETNPLPVKTLLAWMGRIEPEWRPPLCPMTADNQA
                     RLRAVAEKLTLI"
     gene            4133..4978
                     /gene="accD"
                     /locus_tag="PKN80_05500"
     CDS             4133..4978
                     /gene="accD"
                     /locus_tag="PKN80_05500"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNQ35503.1"
                     /translation="MLWKRKKVAVISPRKKVGVRGEDWVKCSACQANLYEKRFLENLW
                     VCPRCQHHHRLTAWQRISITVDAGSFQEADAALRASDPLGFTAVKAYADKVAEAQEET
                     GLTEAALAGRGTIGGFPAELVVTDSNFMMGSLGSVVGEKIVRAVERAVVSNTPLIIIS
                     ASGGGARMYEGMFSLLQMAKTSAALGRLASAGVPYLSVLTDPTMAGVAASFAFLGDIM
                     VAEPGALIGFTGPRVIEQTIRAKLPEGFQRSEFLFRKGALDQVLARREIKPFLVRALS
                     LFHKD"
     gene            5023..5877
                     /gene="lipA"
                     /locus_tag="PKN80_05505"
     CDS             5023..5877
                     /gene="lipA"
                     /locus_tag="PKN80_05505"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNQ35504.1"
                     /translation="MEKRPAWLTKRLVFDPAVQETEAVVARHRLHTVCAAAGCPNRSE
                     CFARRVATFMILGDVCTRGCAFCGVRRGRPEPPAADEPDRVAAAAAALGLEYVVVTSV
                     TRDDLPDGGAGLFARTVTAIRAARPPVRVEVLTPDFQGDRAALETVLSAGPAVFNHNL
                     ETVARLYPRVRPQAGYRRSLEVLRRARRFGSGLVKSGLMLGLGETDAEVESALADLRE
                     AGVEIVTLGQYLRPNPGCLPVTRHPRPEEFDAWAETAKRLGFRAAAAGPFVRSSYYAA
                     EVFRSTGT"
BASE COUNT         1200 a         1925 c         1954 g         1030 t
ORIGIN      
        1 cctgacaccc accagctacc agcaccggga gatgtccctc ttcggaaaag aggcggcccc
       61 ggccggcccg gcgccgcggc caacgatcga ggcgccccgg ccggccgaga gcgaagcgcc
      121 ccgggtggga cggaatgatc cctgcccctg cggaagcggc aaaaagtaca aaaaatgctg
      181 cggtcggaac gcggcttgac aatttaaaat ctgaatttta taatcttaac tcgcctcgcg
      241 agcaaacccc gagcgttctg ctgtgcaaaa cctgttgaca gagacctttt tttctttggg
      301 gcggttatgg gaaaaccctt gatgcacaac gatcttgacc aggtaaatac ggtttggaac
      361 gcggcgctga aacttgttaa gaataaggtg ctcccttcgg ccttttccct ctggatcgaa
      421 ccgctgaaac cggttccggg ccgggagtcg gagctggtcg tggaggcccc ggacggattc
      481 ttcgccgact gggtccaaaa acactacggc gaggtgctgc tggccgcctg ccaggtcatc
      541 aatccggacc tgaagcaggt cctggtccgg gcccggaccg aaaccgggcc ggccgaaccg
      601 ggccggccgg ccgaggtggc cccggccgcc gcgccggtcc gccggcctcc gggaaacggc
      661 aacatcaacc cgctttacac cttcgacaac ttcgtgatcg gggacagcaa ccggctggcg
      721 cacgcggccg ccctttcggt ggcgcagtca cccgggaccg cctacaaccc gctcttcatc
      781 tacggaccgg tgggcgtggg aaagacacac ctgatgcagg ccatcggcaa ccacgcgcgc
      841 catcaggccg gactccaggt ggccttcctc tccagcgaaa agtttctgaa cgagttcatc
      901 gaaagccttc aaaacaagag cacggcggcc ttccggaacc ggttccggga ggtggacctg
      961 ctgttgatcg acgatgtcca gttcctgggc ggcaaggagg agacacagcg ggagttcttc
     1021 cacaccttca acacgctcta cgattttcac aagcaggtca tcgtttccag cgaccggcca
     1081 cccggcgaga tgaaggacat cgaaaaaagg ctggtctccc gttttgagtg gggactggtg
     1141 gtggacatcc agccgcccga cctcgaaacg cgggtggcca tcatcaaaaa caaggcccga
     1201 ctgaagaacc tcgacctgcc tcaggaggtc gccttctgca ttgcggaaaa ggttgaagga
     1261 aatgtccggc tgctggaggg ggcgctcaac aagctgctgg cctgcgcgag cctttacgaa
     1321 aagagcgtgg tcgatcttga gttttcccag agcatcctga acacgctctt tgaggcctcc
     1381 gcggtccggc cgcggatgga ggtgggccgg gtccaggagt ttatcggccg ccactacaac
     1441 ctgaaggttt ccgacctcaa aagcggacga cgccagcagt cgctggtaat gccgcggcag
     1501 ctggccattt acctggcccg gaaacttacc ggagcctcgc ttccacagtt gggcgaggcc
     1561 ttcggcggca aaaaccacac caccatcctt tacacctgtc gaaaagttga ggataagctc
     1621 ggcaaagatc ccgacttcaa ggaagaggta gcccggctgg agaagaagct taaacaggaa
     1681 accaactgaa cggtccgggt tatcaacaga atgttttttc ctggaagcct tgaaaaacat
     1741 aagaaaaccg tctttttcaa cagaaaagac gttctcctgt atttataact actggtttaa
     1801 ttttaaaatt taaaaaggag aagtcgatgg aactgaaagc ccagaagaag gaactaaccg
     1861 ccatcctggg taaggtcatc ggggttttga accccaagcc gaccctgccg aaactcagct
     1921 atgttctgat ggatgccgcc agagagatgc tgaccgtcac cggcaccgac ctcgaaataa
     1981 gcattcggat cgtccgcccc ttaaactcaa ccaaggaagg caaagttttt gtgccgggac
     2041 gaaaactact ggacatcctg cgggagtttc cggaggccga gataaaactg gagaccgaga
     2101 aggagacctt gctgcgtctg agccagggcc gcattaatct ttccctggtc gggttgaagg
     2161 cggaagagtt tccgcagctg gcgaagatca aaaacccgtc ccagttcaag gtgggcagcg
     2221 agatgctcaa gcgggcgctg gccagcacga aaccgatcgt ggtggtggat gagaaccggc
     2281 cccatctttc cggggttttg ctgaccaccg aggatggctg gctgaccgcg gtcgccaccg
     2341 acacccgccg gctggccagg ttcaagacca agacaaacct cgcctcaaac atcaaattca
     2401 tcctgcccac caaggcggcg gaaatcatcc gaagcgtctg ggaggaggag acggagttgg
     2461 acgtgaacct gggaaccaat caggcgatct tctccgggcg cgacatcacc ctgaccaccc
     2521 agatcattga cggaaacttt ccaaattacg cctccatcat tccagccgaa gcggacctgc
     2581 ggccggcgac ggttccggta gagggttttc tggccgcgct gcggcggcta cggcagctgg
     2641 tttcggccgg caagaatatg gttcggctgg acgtgcagcc caagatcatg cgtctttcgg
     2701 gcgaggcggc cggcgtggga gagggaagcg aggacctgga aatcgactac cagggtgagc
     2761 cgatcacagc ctatttcaac ccggactacc tgatggacgg gctgcggccg ctcgagaagg
     2821 aaaccgactt cttcctgggc ctcaacccca acgttgaaaa acccagcctg ctcaggccga
     2881 ccaagagcgg cgactacctg tacctgatca tgccgatgcg gccggtttga agccgcgcgc
     2941 gatggagccg atccgggcca ttgtccgccg ggtccttaag tcggccggcg ggtctccgcg
     3001 tgaggagaag gattacctgg ccggctggga agaggtggtg gctccggaga tccgccgcca
     3061 ttgccgggcc tggtttagga gcgggcggct ggaagtggag gcggacaacc cgaccaccag
     3121 ccattacctg caactgcaga aagagaagat tctggccgcg cttcgaaaac ggcgcctgcc
     3181 gctccgggac ctggtcgtcc ggccccggcg cagcccgcga ccgccggcgg ccgggcgttc
     3241 ctgaaggaga aaacaatgtt tcaaggttcc acggttgcgc tggtcacccc gttccggaac
     3301 ggccggattg acgagaagaa atttcaggac ctggtcgagt ggcagatcgg ggccggaacc
     3361 gacgccctgc tgccctgcgg cagcaccggg gaatcagcca cgctctcgat ggaggagcac
     3421 cagcggctga tcgagttgac gctggaggtt tcggccggcc gtcggaaggt aatcgccgga
     3481 accggctcca acaacaccgc cgaagccctg cacctgaccc agcacgccaa aaaggtcggc
     3541 gccgacgcgg cgctgatcat caccccctac tacaacaagc cgacgccggc cgggctgctg
     3601 ctgcacttct caaccatcgc ccgggcggtg gacatcccga tcatccttta caacgtgccc
     3661 tcccgaaccg gggtttccct gacgccgggg accgtggccg agctggcccg gattcccaac
     3721 atcgtggcca tcaaggaggc ctcgggaagc ctggatcagg tcagttcgat cctcgaccgc
     3781 agcgacatca cggtcctttc gggcgacgac tcgctgacgc tgccgatcat gtcggtaggc
     3841 ggccggggcg tgatctcggt ggcggccaac gtcatcccgg ccggggtggc cgcgctcacc
     3901 cgcgccgggc tggccgggga tctggcgggc gcccaggccg agcatcggcg ctggttcccg
     3961 ctttttcagg cccttttctt cgagaccaac ccgttgccgg taaagaccct gttggcctgg
     4021 atgggacgga tagagccgga atggcgaccg ccgctctgcc ccatgaccgc cgacaaccag
     4081 gcccggctgc gggcggtggc cgaaaaactg acgctgatct gaggcgcgca agatgctctg
     4141 gaaaagaaaa aaagtcgcgg tgatttcacc ccgcaaaaag gttggcgtcc ggggcgagga
     4201 ctgggtcaag tgctcggcct gccaggcgaa tctctacgaa aaaaggtttc tggaaaacct
     4261 ctgggtctgc ccgcgctgcc agcaccacca ccgcctgacc gcctggcagc ggatctccat
     4321 caccgtcgac gccggctcct tccaggaggc ggacgccgcc ctgcgcgcca gcgacccgct
     4381 cgggttcacc gccgtcaagg cctacgccga caaggtggcc gaggcccagg aggagaccgg
     4441 gttgaccgaa gcggccctgg ccggccgcgg gaccatcggg ggcttcccgg ccgaactggt
     4501 ggtaaccgac agcaacttca tgatgggcag cctgggctcg gtggtcggag agaagatcgt
     4561 ccgggccgtt gagcgggcgg tggtttcgaa cacgcccctg atcatcatct cggccagcgg
     4621 cggcggcgcc cgtatgtacg aagggatgtt ttcgctgctt cagatggcca agacctcggc
     4681 ggccctcggc cggctggcct cggccggtgt tccctacctc tcggttctca ccgatccgac
     4741 gatggccggg gtggccgcca gcttcgcctt cctgggcgac atcatggtgg ccgaacccgg
     4801 cgccctgatc ggtttcaccg gaccgcgggt gatcgaacag accatccgcg ccaagctgcc
     4861 ggaaggtttt cagcgttcgg agttcctctt ccgcaagggc gcgctggacc aggtgctggc
     4921 ccggcgggag atcaagccct tcctggtccg ggccctctcc cttttccata aagattgacg
     4981 ccggcgcgga tgccgctttc cggccgccgg ttccagccga agatggaaaa acgccccgcc
     5041 tggcttacga agcgcctggt gtttgatccg gccgttcagg agaccgaagc ggtggtggcc
     5101 cgccaccggc tgcacaccgt ctgcgccgcg gccggctgcc cgaaccggag cgagtgcttc
     5161 gcccgccggg tggccacctt catgatcctg ggcgacgtct gcacccgcgg ctgcgccttc
     5221 tgcggcgtgc gccggggccg accggaaccg ccggcggcgg acgaaccgga tcgggtggcc
     5281 gcggcggccg ccgccctcgg cctggagtac gtggtggtca cctcggtgac ccgggatgat
     5341 ctgcccgacg gcggggccgg gctctttgcc cggaccgtaa ccgcgatccg ggcggcgcgg
     5401 cccccggtcc gcgtggaggt gctcacgcct gatttccagg gggaccgggc cgcgctggaa
     5461 acggtccttt cggcggggcc ggcggtcttc aaccacaacc tggagaccgt ggcccgcctt
     5521 tatccccggg ttcgacccca ggccgggtac cggcggagcc tggaggtgct acggagggcg
     5581 cggcggttcg ggtccggcct ggtgaagagc ggcctgatgc tggggctggg ggagacggac
     5641 gcggaggtcg aatcggccct ggccgacctg cgggaggccg gcgtcgaaat cgtgaccctc
     5701 ggccagtacc tgcgccccaa cccgggctgt cttccggtga ctcgccatcc gcggccggag
     5761 gagttcgacg cctgggcgga gacggcgaaa cggctcgggt tccgggcggc cgccgccggc
     5821 cccttcgtgc gcagctccta ttacgcggcg gaagtttttc gttccaccgg cacctgaggc
     5881 gcccctttgc gccggctgcc gggcgtcggc tataattata ggcggtcaac ccaaaacgaa
     5941 agcgccggcc gggccggcac cctttttccg gaggaagaac atggaagtaa aattgccccc
     6001 gctgggaaac gacatcaagg aggccagcgt ctccttctgg tacaaaaagg tgggcgagag
     6061 cgtcgctgaa ggagaggacc tggtggaact ttccaccgag aaggccagc
//
LOCUS       DAOQYN010000112         6105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_13213_length_6105_cov_4.462479, whole genome shotgun sequence.
ACCESSION   DAOQYN010000112 DAOQYN010000000
VERSION     DAOQYN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13213_length_6105_cov_4.462479"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PKN80_05510"
     CDS             <1..740
                     /locus_tag="PKN80_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNQ35505.1"
                     /translation="VRIFEDYLAAGVRRLHLVFLSAARDGSQSPDEEALLGRLLAVRP
                     AGVELEVGGGIRRWETAERLLSLGVDYLILGTAAVLDIIRLEEERQKAAYEGGLAGFV
                     RQLDFKVTSILQDILERRSAGRFIISLDVRSGAAAVSGWEMTLPPAVDRLAGWFSQLG
                     FTRLIHTAVERDGTLAGPDLEALKSLSDAVPGVSLVAAGGIGSEPDIAALAALKLPNL
                     GGVIVGKALYEGRVDLGRLIRTYQSGS"
     gene            779..1273
                     /gene="ispF"
                     /locus_tag="PKN80_05515"
     CDS             779..1273
                     /gene="ispF"
                     /locus_tag="PKN80_05515"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNQ35506.1"
                     /translation="MVRVGLGFDIHPLVPGRPFRLAGIRIEYERGPLGHSDGDALSHA
                     VGDAILGAGGCGDLGDWFPDNDPRWRGLPGPDLLGLIRDLVAEKGFRPIQVDSILVME
                     KPRLAPFREAVRGGLAGALGLETAAVNVKFRTAEGFGPVGQGEALAAYAVVLVTDNDK
                     EKTG"
     gene            1270..2754
                     /gene="gltX"
                     /locus_tag="PKN80_05520"
     CDS             1270..2754
                     /gene="gltX"
                     /locus_tag="PKN80_05520"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNQ35507.1"
                     /translation="MRVRFAPSPTGYLHLGNARTALFNYLLARQQKGAFVLRIEDTDR
                     ERSRPEFERMILEDLQWLGLDWDEGPDRGGPHAPYRQSERIQAYRERVDWLISEGKAY
                     HCYCSFEELEGMRREQTSRKESPRYDNRCRELTAAQKAAYQREGRRPTVRFRIPDRSL
                     TFFDLIRGDITFDTSLMGDLIIMKSDGLPSFHLAVVLDDIDMEISHVVRGEDHISNTP
                     RHILLFEAFNRKPPSFAHMAMTLGPDGKRLSKRHGATSVRELRNQGYLPEAVFNYLAL
                     LGWGINDDQEVFSRQELIDRFSLDRCKEGAAIFDQAKLTWLNGLYMQKKGPDRITELA
                     LPYLQEAGLAASGAGPEERTRLARIVGLIGDRVKKLNEIPEAVAIFVRETVDYDPAAV
                     EKVLRKPGVAEILAGMLPLFEAADDFSAGRLEALVREFCQARALKTGQVFHPVRVAAT
                     GRTVGPGLFELLEVLGREKVLRRLRRAVEFLASGEPPAAGTGVS"
     gene            complement(2747..3331)
                     /gene="metW"
                     /locus_tag="PKN80_05525"
     CDS             complement(2747..3331)
                     /gene="metW"
                     /locus_tag="PKN80_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNQ35508.1"
                     /translation="MENLSPEYRVIVDLVRPNSTVLDLGCGNGRLLCLLKQEKEVKGQ
                     GIEISLEKISECVHQGVNVLHGDLDNGLAEYPDKSFDYVILNNSIPELRSPRIAIKEG
                     LRVGRELIVGFPNFAHWRARCQLGLFGRVPVTDSLPHQWFDTPNRHFLSINDFVAFCR
                     SERIRVNSSIHLCGRRRVRFAPNLLADYALFLLN"
     gene            complement(3371..4501)
                     /locus_tag="PKN80_05530"
     CDS             complement(3371..4501)
                     /locus_tag="PKN80_05530"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNQ35509.1"
                     /translation="MKTAAVPDLISPKKFTFAVPPDRLKLESGQYLGPIDIQYETYGK
                     LNPDRSNAILLLHALSGDSHVAGRYRADDRKPGWWDEMVGPGRAFDTNRYFVICSNVL
                     GGCRGSTGPASLNPETGRPYGLQFPVITIGDIVNCQRRLVDHLGIEKLYCLAGGSMGG
                     MQVLQWLAAYPERIAMAIPIATTLRHSPQQIAFDAVGRQSIMADPAWNGGDYYGREVP
                     ARGLAIARMVGHITYMSEKSMEEKFGRQLKDKKYAFSFSTEFEVEGYLDYRGDSFVKR
                     FDANSYLYLTKAMDYFDLEADFGRNLFRGLAARILVLSFSSDWLYPPGQSKEIVRVMK
                     RQGGEVTYCEIPSSYGHDSFLVEIAEETRLISDFLAAGSKLL"
     gene            complement(4498..5433)
                     /gene="cysK"
                     /locus_tag="PKN80_05535"
     CDS             complement(4498..5433)
                     /gene="cysK"
                     /locus_tag="PKN80_05535"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNQ35510.1"
                     /translation="MNIYPDISRAVGNTPLVQLNRIGRETGARLVLKLEFFNPLSSVK
                     DRIGAAMIDAAERDGRLRPGGTIIEPTSGNTGIALAFVSAARGYRLILTMPETMSLER
                     RALLKILGAEVVLTPGPEGMSGAIRKAGELLLERPGAFMPQQFENPANPEAHRRTTAE
                     EIWRDTGGQVDLLVAGVGTGGTITGVTEVIKARRPGFRAVAVEPAESPVLSGGKPGAH
                     RIQGIGAGFVPAVYRPDLIDEVIQIGSDEAGEYARRLAREEGILAGISSGAATAAALK
                     VAGRPENRGKLILVIIPSCGERYLSTWLFADYMKQ"
     gene            complement(5492..>6105)
                     /locus_tag="PKN80_05540"
     CDS             complement(5492..>6105)
                     /locus_tag="PKN80_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNQ35511.1"
                     /translation="RYPEISGPDASYHGLSYWKSFGRFPAAGNRSIAYTVKARVQLLR
                     DLGCCLSPFNSFLILQGVETLPLRVRQHSTNALEIARWLEKHPAVAWVNYPGLESHPD
                     HQRAVRYFREGFGAIIGFGIKGGLKASQKLIDSVRLVSHLANIGDAKTLVIHPASTTH
                     QQLTGAERARAGVTDDYIRLSVGLENCADIKADLDQALRASQS"
BASE COUNT         1036 a         1884 c         2082 g         1103 t
ORIGIN      
        1 cggttcggat ctttgaagac tacctggccg ccggggtgcg gcgcctgcac ctggtttttc
       61 tctcggccgc ccgggatgga agccagagcc cggacgagga agcgctgctc ggccggctgc
      121 tggcggtccg gccggccggc gtcgaactgg aagtcggcgg cggcatccga aggtgggaaa
      181 cggccgaacg gcttctctcc ctgggcgttg attacctgat cctgggtacg gcggccgttc
      241 ttgacataat acgcctggag gaggaacggc agaaggccgc ttacgagggc gggctggccg
      301 gttttgtccg gcagctggat ttcaaggtaa cctcgattct ccaggacatc ctggaacggc
      361 ggtccgccgg gagattcatc atcagcctcg atgtccggag cggtgcggcg gcggtaagcg
      421 gctgggaaat gacccttccc ccggcggtcg accggctggc cggctggttt tcccagctgg
      481 gctttacccg tcttattcac accgcggtgg aacgggacgg gaccctggcc ggaccggacc
      541 tggaggcgct caagtcgctc tcggacgccg ttcccggggt ttccctggtc gccgccggcg
      601 gaatcggttc agagccggat atcgccgccc tggccgcttt gaaactgccc aacctgggcg
      661 gggtaatcgt cggcaaggcc ctttacgagg ggcgggtcga cctgggccgg ctgatcagga
      721 cataccagtc cgggagctga accgcgcccg ggttcgatcg tttcagggag ggagaaaaat
      781 ggtacgagtc ggcctcggtt ttgatattca cccgctggtg ccggggcgac ccttccggct
      841 ggccggcatc cggatcgagt acgagcgggg tccgctcgga cacagcgacg gcgatgccct
      901 ttcccatgcc gtcggcgacg ccatcctggg ggccggcggc tgcggggacc tcggggactg
      961 gtttcccgat aacgacccgc gctggcgcgg cctgcccggc ccggacctgc tcggcctgat
     1021 ccgggacctg gtcgccgaaa agggattccg gccgatccag gtggattcca tcctggtcat
     1081 ggagaagccc cgcctggccc ccttccggga agcggtccgc ggcggcctgg ctggagcgct
     1141 cggcctggag acggcggcgg taaacgtcaa gttccggacg gccgagggct ttggcccggt
     1201 cggccagggg gaggccctgg ccgcttacgc ggtcgtgctg gtgaccgaca acgacaagga
     1261 gaaaacagga tgagagtgag atttgcaccc agtcccaccg gttacctgca cctgggtaat
     1321 gcccggaccg cccttttcaa ctacctgctg gcgcgccagc agaagggggc gtttgtcctg
     1381 cggatcgaag ataccgaccg ggagcggagc cgtcccgagt ttgaacgaat gatcctggaa
     1441 gacctccagt ggcttggcct ggactgggac gagggtcccg accggggcgg cccccacgct
     1501 ccctaccgcc agtcggaaag gatccaggcc taccgggaac gggtggactg gctgatcagc
     1561 gaggggaagg cttaccactg ctactgcagc ttcgaggagc tggaggggat gcgccgggag
     1621 cagaccagcc ggaaggagag tccccgttat gacaaccgct gccgggagtt gacggccgcc
     1681 cagaaggccg cctaccagcg ggagggaagg cgtcccacgg tccgtttccg gataccggac
     1741 cggagcctga ctttttttga cctgatcagg ggcgatatca ccttcgacac cagcctgatg
     1801 ggtgacctga tcatcatgaa atcggacggg ctcccttctt ttcacctggc ggtggtcctc
     1861 gacgacatcg acatggaaat cagccacgtg gtccggggcg aggaccacat ttccaacacg
     1921 ccgcgccaca tcctgctctt cgaggccttc aaccggaaac cgcccagttt cgcccacatg
     1981 gccatgacgc tcggcccgga cgggaagcgc ctctccaagc gtcacggggc cacctcggtg
     2041 cgggaattga ggaaccaggg ctacctgccc gaggcggtct tcaattacct ggccctgctg
     2101 ggctggggga tcaacgacga ccaggaggtc ttcagccgcc aggaactgat cgatcggttc
     2161 agcctggatc ggtgcaagga gggggcggcc atcttcgatc aggccaagct cacctggctg
     2221 aatggtttat acatgcagaa gaagggaccg gatcgaatca cggaactggc cctgccctac
     2281 ctgcaggagg ccggcctggc cgcttccggg gccggcccgg aggagcggac ccggctcgcc
     2341 cggatcgtcg gcctgatcgg cgaccgggtg aagaagctga acgagatccc ggaagcggtc
     2401 gcgatctttg tcagggagac ggtcgattac gatccggccg ccgttgaaaa ggtgctccgg
     2461 aaaccgggcg tcgcggaaat actggcgggg atgctgccgc tcttcgaggc ggccgatgat
     2521 ttttcggcgg gacggctgga ggcgctggtg cgtgaattct gccaggcgcg cgccctgaag
     2581 accggccagg tttttcatcc ggtccgggtg gccgccaccg ggcggacggt ggggccgggc
     2641 ttatttgaac tgctggaagt cctgggtcgg gagaaggtcc tgcggcggct ccggcgggca
     2701 gtcgagtttc ttgcctcggg ggagccgccg gcggcgggga ccggggtcag ttgagcagga
     2761 aaagcgcgta gtcggccagc aggttggggg cgaagcggac gcgccggcgg ccgcagaggt
     2821 ggatgctgga gttcacccgg atcctctcgc tgcggcaaaa agccacgaaa tcgttgatgc
     2881 tcagaaagtg gcggttcggg gtgtcaaacc actggtgcgg gagcgaatcg gtaaccggca
     2941 cccggccgaa cagcccgagc tggcagcgcg ccctccagtg ggcgaagttg ggaaaaccga
     3001 caatcagttc ccgtcccacc ctcaggccct ccttgatggc gatccgcgga ctgcgcagtt
     3061 cgggaatgct gttgttgaga atcacgtaat cgaaactctt gtccgggtat tcggccagcc
     3121 cgttgtccag gtcgccgtgg agcacgttga ccccctggtg gacgcactcg gagatcttct
     3181 ccaggctgat ttcgatcccc tgtcccttca cctctttttc ctgcttgagc aggcagagca
     3241 gccgtccgtt tccgcagccc aggtccagga cggtgctgtt cggccggacc aggtccacga
     3301 tcacccggta ttcagggctc aagttttcca tggttgatta aaaaccgctc cttttccgcg
     3361 gcggatccgg tcagagcaat ttcgagccgg cggccaggaa gtccgaaatc aatcgggttt
     3421 cctcggcgat ctcgaccagg aaggagtcat gtccgtagct ggacgggatt tcgcaatagg
     3481 tcacctcgcc tccctgccgc ttcataaccc ggacgatctc cttcgattgg ccgggcgggt
     3541 aaagccagtc ggaactgaaa ctcaggacca ggatccgggc cgccagtccc cggaagaggt
     3601 tgcggccgaa atcggcctcg agatcgaaat aatccatggc cttggtcagg taaagatagg
     3661 aattggcgtc gaaacgcttg acgaaggaat caccccggta atccaggtat ccctccactt
     3721 cgaattcggt ggagaaggag aaggcgtact tcttgtcctt cagctggcgg ccgaacttct
     3781 cctccatcga cttttcgctc atgtaggtga tatgccccac cattcgggcg atggccagtc
     3841 cccgggccgg gacttcccgt ccataataat cgcccccgtt ccaggccggg tcggccatga
     3901 tcgattgacg gcctaccgcg tcaaaggcga tctgctgggg cgaatggcgc agggtggtgg
     3961 cgatcgggat ggccatcgcg atccgttcgg gataggcggc cagccactgg agaacctgca
     4021 tgcctcccat ggaaccgccg gccaggcaat aaagcttttc gatgcccagg tggtcaacca
     4081 ggcgccgctg gcagttcacg atatccccga tggtgatgac cgggaactgg agtccgtaag
     4141 gccggccggt ttccgggttg aggctggccg gcccggtgct gccgcggcag ccgcccagga
     4201 cgttggagca gatcacgaaa tagcggttgg tgtcgaaagc ccgcccggga ccgaccatct
     4261 cgtcccacca gccgggcttc cggtcatctg cgcggtagcg tccggccacg tggctgtcac
     4321 cggagagcgc gtgcaggagg aggatcgcgt tgctccggtc cgggttcaac ttcccgtagg
     4381 tctcgtactg gatgtcgatc gggcccaggt attggccgct ttccagcttc agccggtccg
     4441 gcgggacggc gaaggtgaat tttttagggc tgatcaggtc ggggacggcg gcggttttca
     4501 ttgtttcatg taatcggcga agagccaggt ggacaggtag cgttcgccgc agctggggat
     4561 gatgaccagg atcagtttgc cccggttttc cgggcggccg gccaccttga gggcggccgc
     4621 ggtggccgcg ccgctcgaga tcccggccag gatgccctcc tcgcgggcga gacgccgggc
     4681 atattcgccg gcctcgtcgc tgccgatctg gattacctcg tcgatcaggt cgggccggta
     4741 gacggccggc acgaagccgg ccccgatgcc ctggatgcgg tgggcgcccg gtttaccgcc
     4801 cgacagcacc ggtgattcgg ccggttccac cgccaccgcc cggaagcccg gccgccgcgc
     4861 cttgatcacc tcggtcaccc cggtgatcgt ccccccggtt cccacgcccg ccaccagcag
     4921 gtcgacctgg ccgccggtgt cacgccagat ctcctcggcg gtcgtccggc ggtgcgcctc
     4981 ggggttggcc gggttttcaa actgctgcgg catgaaagcg cccggtcttt ccaggaggag
     5041 ttccccggct ttcctgatgg ccccggacat cccttccgga cccggggtga gcaccacttc
     5101 cgcgcccagt atcttcagca gggcccggcg ttccaggctc atcgtttcag gcatggtcag
     5161 gatgaggcgg taaccccggg ccgccgagac gaaagccagg gcgatgccgg tattgccgct
     5221 ggtcggctcg atgatggtgc cgccggggcg gagccggccg tccctctccg cggcgtcgat
     5281 catggccgcc ccgatccggt ccttgacgct ggagagcggg ttgaagaact ccagcttcag
     5341 gaccaggcgg gcgccggttt cacgcccgat gcgattgagc tggaccagcg gggtatttcc
     5401 caccgcccgg ctgatgtccg gataaatatt catgacggct cccgcgattg tgattggaaa
     5461 cttcggatat tatagcacgg ccgccggtcc gtcaggactg gctggcgcgc agcgcctggt
     5521 cgaggtcggc cttgatgtcg gcgcagtttt ccaggccgac cgaaagccgg atatagtcgt
     5581 cggtcacccc ggcccgggcc cgctcggcgc cggtcagctg ctggtgcgtg gtggaggctg
     5641 ggtggatgac cagcgtcttg gcgtcgccga tgttggccag gtgtgatacc aggcgcaccg
     5701 agtcgatcag cttctgactg gccttcaagc cgcccttgat gccgaaaccg atgatcgccc
     5761 cgaagccttc cctgaagtag cggaccgccc gttgatgatc cggatggctc tcaagtccgg
     5821 gatagttgac ccaggccacg gccgggtgct tttccagcca gcgggcgatt tcaagggcgt
     5881 tggtcgaatg ctggcgaacc ctgagcggga gcgtctcgac gccctgcagg atcaggaagg
     5941 aattgaaggg agagaggcag cagccgaggt cacgcaggag ctgcacccgg gccttgaccg
     6001 tgtaggcgat ggaacggttc ccggccgccg ggaaacggcc gaatgacttc cagtaggaga
     6061 ggccgtggta ggaggcgtcc ggtccggaaa tttcgggata acgtc
//
LOCUS       DAOQYN010000113         6101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_13226_length_6101_cov_3.857757, whole genome shotgun sequence.
ACCESSION   DAOQYN010000113 DAOQYN010000000
VERSION     DAOQYN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13226_length_6101_cov_3.857757"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKN80_05545"
     CDS             complement(<1..318)
                     /locus_tag="PKN80_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNQ35512.1"
                     /translation="MREQACGDRREGIPSYYLRGQAGGCTARLKEIVTFLDDTLETNS
                     IRDRSWNGLQVEGRPEVGRIIFAVDAGLATFQKAAGLRADLVVVHHGLFWKNNNPVCV
                     GTMK"
     gene            583..1557
                     /locus_tag="PKN80_05550"
     CDS             583..1557
                     /locus_tag="PKN80_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35513.1"
                     /translation="MRKLGVGCYGTSGHQVLGQLVSHPRAELVAAAGVPADRIRDLFG
                     ERAGEVTLYPDLDSLLSDKRVELVSLCSPVRFDQPAEVLAVLESGRHALAEKPAAFSR
                     ADLDNLVSTAGRRGVHFREMGGVEFTPPLPEIKELVISGALGEIVQAWAMKSYPYHDR
                     RSQDPRRDGGIMQAGVHAVRFICATAGLRVRKAAGFVTGKANPVSGGRLQMAASVALE
                     FDTGAVGGLVINYLNPAGLGAWGNDQLRLHGFRAMAETTDGFTRGRLVDAGGERALAT
                     IGRKADRTYLDYYADFLLDGTPMPTPFEKELGFMEDSIKIMEALYLPL"
     gene            1554..2759
                     /locus_tag="PKN80_05555"
     CDS             1554..2759
                     /locus_tag="PKN80_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35514.1"
                     /translation="MNTSRERVLAALSHREPDRVPVDLGAMDSTGITAIAYNRLKKHL
                     GLAAGHTRIFDPYQQVVKVEESVYRHIPGDVRPLMPEPDRWKPVLLADGSAAEVPERW
                     NPETQPDGSQVIRDAAGAIRSRMPAGGYYFEFVNPPLQHLEEPAGLDEHRALIENFDW
                     PGFCDGGFEAMAGRAEALERQGQYAVMGNFAVHFFAAGQLLRGFEQFLVDLAINPEMV
                     HGLLGRLADAYLERFERFHAALAGRVQVVNVNDDLGSQEGLLLSPALYRKLVRPYQER
                     LYRRIRECGYHLFLHTDGSVYDLIPDFIEMGVDILNPVQFTCRNMELEKLKREFGRDL
                     TFWGGGIDTQNVLPRATPAQVREAVKRSLALLAPGGGFVFNQVHNIQPDVPPENILAM
                     YEAVREFGG"
     gene            2789..3982
                     /locus_tag="PKN80_05560"
     CDS             2789..3982
                     /locus_tag="PKN80_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35515.1"
                     /translation="MSSEIDRDLLEALSSYDPSVRSAAISEAARRLESGRLAPAAEAR
                     PWLNLHLHTFHSFHYRDWSPARVILECRRLGLLGAGTVDFDTLAGLEETFAAGRAFGL
                     PVLTGFESRVFVPEYRDRVLNSPGEPGIYYFCGLGFAARPDPASAAGRFLDGLRATAQ
                     ARNRKVIERLNHFLGRVTLDYQADLLPLTPSANPTERHIILAYLRKAEALLAGGADSF
                     WAGVLKIGPEAAAAYRGSPVEFQELLRARLVKRGGPGYIEPEARDFPVLDEVVAATRA
                     AGGRPVATWLDGTSPGESDPGQWLDFFIGRGLEAVAIIPERNWNLRDPEEKRRKTANL
                     GQFLAACRRRDFPVLCGTEMNRSGQPLVDDFSQPELAGHLDYFLASAGRFLPGLARRQ
                     PEMKT"
     gene            3979..5049
                     /locus_tag="PKN80_05565"
     CDS             3979..5049
                     /locus_tag="PKN80_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNQ35516.1"
                     /translation="MKAAVYHGIQDVRFEEVPAPACGPAEILVGVNYCAVCGTDVRIY
                     LHGHKNVTPPAVTGHEITGRVLEIGPEVKEPGLAVGDRVTLVTSIGCGRCKMCRRGLY
                     NLCPDTRAIGYRYPGGFAERLAVPAEAVRQRAVLKLPDNLSLLEGALIEPLSCAINGQ
                     NYLQVRPDDTVVIYGGGPIGFMHAALALAVPAARVIMIDPAFDRLKHFGREFPGLILW
                     DPKEVRVADEVNKLTGGFGADVVITACPSPQAQVEGLGLLGSRGRISLFGGLPKEAGR
                     IEIDANLVHYKELSVYGAFASNRADYLEAARLLSERKIEAEKFISSVRPLSEFPVAVE
                     LIRSGAVLKVVLEVKKDGDANA"
     gene            5030..5824
                     /gene="srlD"
                     /locus_tag="PKN80_05570"
     CDS             5030..5824
                     /gene="srlD"
                     /locus_tag="PKN80_05570"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HNQ35517.1"
                     /translation="METQTLKGRVAIVTGAARGLGEAIAHGLAAEGAIVCPWDINIEG
                     VRQVAGKIKAAGFQAGRPAAVDVTEADAVRSEVESIARELGRLDILVSNAGILKVHEV
                     TEFPEAEWRRVIEVNLTGYFICAKYAALVMKRQRSGSIIQVNSKSGKKGSYRNSAYAA
                     SKFGGIGLTQSLALDLAPHQVRVNAVCPGNLLDSPLWQESLFDEYARKWNISREEVRL
                     KYTGQVPLGRPCTYQDVINVVVFLAGDASSYMTGQAINVTGGQEMK"
     gene            complement(5773..>6101)
                     /locus_tag="PKN80_05575"
     CDS             complement(5773..>6101)
                     /locus_tag="PKN80_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35518.1"
                     /translation="GRFKSCLVDSERYLLIVHRYIELNPIRAAMATSPEHYRWSSAAA
                     NSSSMEAKSSERFRTTLSFFAIALLQKGPTRPGRMIPRRPARRPRPGPYFISWPPVTL
                     MAWPVM"
BASE COUNT          956 a         2238 c         1912 g          995 t
ORIGIN      
        1 cttcatcgtg ccgacgcaga ccgggttgtt attcttccag aagaggccgt ggtggaccac
       61 caccaggtcg gcccgcagtc cggccgcctt ctggaaggtc gccaggccgg cgtccaccgc
      121 gaagattatc cgcccgacct ccggccgccc ttcgacctgg aggccgttcc aggatcggtc
      181 ccggatggaa ttcgtttcca gggtatcatc caggaaggtt acaatctctt tcagccgtgc
      241 cgtacaccct ccagcctgtc ccctcaagta gtaggagggg atcccctcac gcctgtcccc
      301 gcatgcctgt tcccgcatgc agtaggcggg catgtccgac gaagcctttt gggcgtagtc
      361 ggaaatctaa gttcaatgta ttttagtaat ccccagtagg cggggatcca ggttacatgt
      421 cttgcattaa cctcttaggt ggggacccag ttgttggttt ttagtccgtc tttttctaaa
      481 cccgccgctc aacgagattt taccacacgg ccatttttgt tttaaaataa aggaatcaag
      541 gcggactgcc gccattttta aaaccttcct caagggcagc ccatgcgcaa actcggagtc
      601 ggctgttacg gcaccagcgg ccaccaggtc ctgggccagc tggtctccca tccgcgagcg
      661 gagctggtgg ccgcggccgg cgtgccggcc gaccggatcc gcgacctctt cggcgaacgg
      721 gccggcgagg tcaccctcta cccggacctc gattccctgc tttccgacaa gcgggtggag
      781 ctggtctccc tctgttcgcc ggtccgcttc gaccagccgg ccgaggtcct ggccgtcctg
      841 gaatcgggtc gccacgccct ggccgagaag ccggccgctt tcagccgggc cgatctcgac
      901 aatctcgtct cgaccgccgg ccgccgcggg gtccacttcc gcgagatggg cggtgttgaa
      961 ttcaccccgc ccctgccgga gataaaggag ctggtgatct ccggcgccct gggcgaaatc
     1021 gtccaggcct gggccatgaa gtcctacccc taccacgacc gccgcagcca ggacccgcgg
     1081 cgcgacggcg gcatcatgca ggccggcgtc cacgccgtgc gtttcatctg cgccaccgcc
     1141 ggcctgcgcg tccgcaaggc ggccggcttc gtgaccggca aggccaaccc ggtctccggc
     1201 ggccgcctcc agatggccgc cagcgtcgcc ctcgagttcg acaccggcgc ggtgggcggc
     1261 ctcgtcatca actacctcaa cccggccggc ctgggcgcct ggggcaacga ccagctgcgc
     1321 ctgcacggct tccgcgccat ggccgagacc accgacggtt tcacccgggg ccgcctggta
     1381 gacgccggcg gggaaagggc cctggccacc atcggccgga aggcggaccg gacctacctc
     1441 gattactacg ccgatttttt gcttgacggg accccgatgc cgacgccatt cgaaaaggag
     1501 ctcggtttta tggaagattc gatcaagatc atggaagccc tctacctgcc cctatgaata
     1561 cttcacgcga acgggtcctg gccgccctct cccaccgcga accggaccgg gtcccggtgg
     1621 acctgggcgc catggattcc accggcatca ccgccatcgc ttacaaccgc ctgaagaagc
     1681 acctcggcct ggccgccggc catacccgga tcttcgatcc ctaccagcag gtggtcaagg
     1741 ttgaggagag cgtttaccgc cacattcccg gcgatgtccg gccgctcatg cccgagccgg
     1801 accgctggaa gccggtcctc ctggccgacg gctcggccgc cgaggtgccg gagcgctgga
     1861 acccggaaac ccagcccgac ggttcccagg tcatccgcga tgcggccggg gccatccgtt
     1921 cccggatgcc ggccggcggc tactacttcg agttcgtcaa cccgcccctc cagcacctgg
     1981 aggagccggc cgggctcgat gaacaccgcg ccctcatcga gaacttcgac tggcccggct
     2041 tctgcgacgg cggcttcgag gccatggccg gccgcgccga ggccctggag cggcaggggc
     2101 agtacgcggt catgggcaac ttcgccgtcc acttcttcgc cgccggccag ctgctgcgcg
     2161 gcttcgagca gttcctggtc gacctggcca tcaaccccga gatggtccac ggactgctgg
     2221 gccgcctggc cgacgcctac ctggagcgct tcgagcgctt ccacgccgcc ctggccggcc
     2281 gcgtccaggt ggtcaacgtc aacgacgacc tcggcagcca ggaggggctc ctcctctccc
     2341 cggccctcta ccggaaactc gtccgtccct accaggaacg cctctaccgc cgcatccgcg
     2401 agtgcggcta ccatctcttc ctgcacaccg acggctcggt ctacgacctc atccccgact
     2461 tcatcgagat gggcgtcgac atcctcaacc cggtccagtt cacctgccgc aacatggagc
     2521 tggagaagct gaagcgcgag ttcggccgcg acctcacctt ctggggcggc ggcatcgaca
     2581 cccagaacgt cctgccccgg gccaccccgg cccaggtcag ggaggccgtc aagcgcagcc
     2641 tggccctgct ggcccccggc ggcggctttg tcttcaacca ggtccataac atccagcccg
     2701 acgtgccgcc cgaaaacatc ctggccatgt acgaggccgt ccgggagttc ggcggctgag
     2761 gccgccggcc ggaaagccgg aaagagccat gagcagcgaa atcgaccgcg atctcctcga
     2821 agcgctttcc agctatgacc cctccgtccg ctcggcggcc atctccgagg cggcccgccg
     2881 gctggagtcc ggccggctcg ccccggcggc cgaagcgcgc ccctggctca acctgcacct
     2941 gcacaccttc cactccttcc actaccgcga ctggtccccg gcccgggtca tcctcgagtg
     3001 ccggcgcctc ggcctgctcg gcgccggtac ggttgacttc gacaccctgg ccggcctcga
     3061 ggagaccttc gccgccggcc gggccttcgg cctgccggtg ctgaccggtt tcgagagccg
     3121 ggtcttcgtg cccgagtacc gcgaccgcgt cctcaactcg cccggcgagc cgggcatcta
     3181 ttacttctgc ggcctgggct ttgcggcccg gcccgacccg gcctcggcgg ccggccgctt
     3241 cctggacggc ctgcgggcca ccgcccaggc ccgcaaccgc aaggtcatcg agcggctcaa
     3301 ccatttcctg ggccgggtca ctctcgatta ccaggcggac ctgctccccc ttaccccctc
     3361 ggccaacccg accgagcggc atatcatcct ggcctacctg cgcaaggccg aggcgctcct
     3421 ggccggcggg gccgattcct tctgggccgg ggtcctgaag atcggccccg aggcggcggc
     3481 cgcttaccgc ggctctccgg tcgaattcca ggagctcctc cgggcccggc tggtcaagcg
     3541 cggcggcccc ggctacatcg agcccgaggc gcgcgatttc ccggtgctgg acgaggtggt
     3601 ggccgccacc cgggccgccg gcggccggcc ggtggccacc tggctcgacg gcaccagccc
     3661 cggcgagtcc gatcccggcc agtggctcga cttcttcatc ggccggggcc tggaggcggt
     3721 ggccatcatc ccggaacgca actggaacct cagggacccg gaggagaagc ggcgcaagac
     3781 cgccaacctc gggcaattcc tggccgcctg ccgccgccgc gatttcccgg tcctctgcgg
     3841 caccgagatg aaccgttccg gccagccgct ggtcgatgac ttcagccagc cggagctggc
     3901 cggccacctg gactacttcc tggcctccgc cggccgtttc ctgccggggc tggcccgccg
     3961 tcaaccggag atgaaaacat gaaagccgcc gtttaccatg gcatccagga cgtgcgtttc
     4021 gaggaggtcc cggcgccggc ctgcggcccg gccgaaatcc tggtcggggt caactactgc
     4081 gccgtctgcg gcaccgacgt ccgcatctac ctgcacggcc acaagaacgt caccccgccg
     4141 gcggtcaccg gccacgagat taccggccgg gtgctggaga tcggccccga agtcaaggag
     4201 cccggcctgg ccgtcggcga ccgggtcacc ctggtcacct cgatcggctg cggccggtgc
     4261 aagatgtgcc gccgcggcct ctacaacctc tgtcccgaca ccagggccat cggctaccgc
     4321 taccccggcg gcttcgccga gcggctggcc gtgccggccg aggccgtccg ccagcgggcg
     4381 gtcctcaaac tgcccgacaa cctttcgctg ctcgagggcg ccctcatcga gcccctctcc
     4441 tgcgccatca acggtcagaa ctacctccag gtccgtcccg acgacaccgt cgtcatctac
     4501 ggcggcggtc cgatcggctt catgcacgcc gcgcttgccc tggccgtgcc ggccgcccgg
     4561 gtcatcatga tcgacccggc cttcgaccgg ctgaagcact tcggccggga gttccccggc
     4621 ctcatcctct gggacccgaa ggaggtccgg gtggccgacg aggtgaacaa gctcaccggc
     4681 ggcttcggcg ccgacgtggt catcaccgcc tgcccctcgc cccaggccca ggtcgagggc
     4741 ctcggcctgc tcggctcccg cggccgcatc agcctcttcg gcggcctgcc gaaggaggcc
     4801 ggccggatcg agatagacgc caacctggtc cactacaagg agctctcggt ttacggcgcc
     4861 ttcgcctcca accgggccga ctacctggag gcggcccgcc tcctctccga gcggaagatc
     4921 gaggccgaaa agttcatcag ctccgtccgg ccgctctccg aatttcccgt cgcggtggaa
     4981 ctgatccgtt ccggcgcggt tctgaaggtc gtcctggagg tcaagaagga tggagacgca
     5041 aacgcttaag ggaagggttg ccatcgtcac cggcgccgcc cgcggcctgg gcgaggccat
     5101 cgcgcacggc ctggccgccg agggggccat cgtctgcccc tgggacatca atatcgaggg
     5161 cgtgcgccag gtggccggca agatcaaggc ggcgggcttc caggccggcc ggccggccgc
     5221 ggttgacgtc accgaggcgg acgcggtcag aagcgaggtt gagagcatcg cccgggagct
     5281 gggccgcctc gacatcctgg tctccaacgc cggcatcctc aaggtccacg aggtgaccga
     5341 gtttcccgag gcggaatggc gccgggtcat cgaggtcaac ctgaccggct acttcatctg
     5401 cgccaagtac gcggccctgg tcatgaagcg gcagcgttcc ggctccatca tccaggtcaa
     5461 ctccaagtcc ggcaagaagg gcagctaccg gaacagcgcc tacgccgcca gcaagttcgg
     5521 cggtatcggc ctcacccaga gcctggctct cgacctggcc ccccaccagg tccgggtcaa
     5581 cgccgtctgc cccggcaacc tgctcgacag ccccctctgg caggagtccc tcttcgacga
     5641 gtatgcccgc aagtggaaca tctcccgcga ggaggtgcgc ctcaagtaca ccggccaggt
     5701 gcccctgggc cgcccctgca cctaccagga cgtgatcaac gtggtcgtct tcctggccgg
     5761 cgacgcctcc tcctacatga ccggccaggc catcaacgtc accggcggcc aggagatgaa
     5821 atagggtccg ggccttggac gccgcgcggg ccggcgtggt atcatccggc ccggccgggt
     5881 tgggcctttt tggagcaggg caatggcgaa aaaggacaag gtggttctga accgttcgga
     5941 ggatttcgcc tccatcgagg acgagttggc ggccgcgctg gaccagcggt agtgctccgg
     6001 ggaggtggcc atcgccgcgc ggatcgggtt gagttcgata tagcggtgca cgataagcag
     6061 gtagcgttcg gaatccacca ggcaggactt gaagcgccct t
//
LOCUS       DAOQYN010000114         6095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_13241_length_6095_cov_4.913907, whole genome shotgun sequence.
ACCESSION   DAOQYN010000114 DAOQYN010000000
VERSION     DAOQYN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13241_length_6095_cov_4.913907"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PKN80_05580"
     CDS             complement(<1..666)
                     /locus_tag="PKN80_05580"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNQ35519.1"
                     /translation="MAEDRRLLDPAVLARLSNLYLVARTVVEGSLTGLHRSLHKGSSV
                     EFAEHREYAPGDDPRYLDWRVFGRTDRLYVKTFREETNLKSLILLDASGSMAYRGRGL
                     SKLEYAVYLAAGLSYLMVNQKDAVGLMVFDSAVRRMVKPASTSRHLHHLLEQLQVTAA
                     AETTNLGEVLHTIAARARRRSLIILISDLLDDPERVLKGLVHFRHKYHEVLVFQVLDP
                     VEQS"
     gene            complement(670..1656)
                     /locus_tag="PKN80_05585"
     CDS             complement(670..1656)
                     /locus_tag="PKN80_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ35520.1"
                     /translation="MDEKLRLLEDLKKTREGLIREMHRVIIGQDEIIEKIMAGLFAGG
                     HCLLIGVPGLAKTLMVRTLAKILDLSFKRIQFTPDLMPSDITGTELIQEDPVSRVRNF
                     RFRPGPVFANLVLADEINRATPKTQAALLEAMQEYQVTAAGNAYRLPLPFLVLATQNP
                     IELEGTYPLPEAQLDRFMFSVHLDYPAAEEEKRIAEQTVSELNPEVKNLLECGRILEF
                     QSLVREIPVSDYVLGYAVKLTRQSRPNNGEAPEFIRNFVSWGAGPRAAQYLVLGAKAA
                     AALRGDVNVSVADIRSVAMNVLRHRIFTNFNADAEGLDSSWIVTRLLETFPD"
     gene            complement(1679..3094)
                     /locus_tag="PKN80_05590"
     CDS             complement(1679..3094)
                     /locus_tag="PKN80_05590"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNQ35521.1"
                     /translation="MLLLVFAATPAGAWECGKAPPVQTPQTRKAGEAVPPLPLPATPL
                     RRSEKKNPPSPPVLVGKVVCGPDDRWTRAENDVDNLLRLASQQLSVPYRAVRIDLARF
                     SFDPEEVPILYLTSVEPFTPGDNLLPRFKNYLEKGGFIWANASSGSPEFVRSLADWLG
                     KIYPDRNLYPLYPNHPLKHAFHNLEKVGIMENGVKASGSPDLRVLNLGCRAAVIVSGL
                     DLGCGWALHTHDFGRRYLPEDAVRIGVNLVSYCLAWIEFGKLYGQMPVYAEKTRKKSG
                     KIYLGQVMHAGDWDPHPAALGRLLYRVAEEAKVPVYLERINVDLKKDPLDDIPILYLT
                     GHFDPRLSRPEIEKLRAFLRRGGTLIADSCCGSQEFGAAFRKLMAELLPGIEPARWET
                     GHPVYRAPYRLESFLYTDATPGNQGPLEVYLLDGLPAAVFSAHGLGGGWEGIARPYTS
                     GLSASQSGQLGVNLIAYLLTH"
     gene            complement(3187..>6095)
                     /locus_tag="PKN80_05595"
     CDS             complement(3187..>6095)
                     /locus_tag="PKN80_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35522.1"
                     /translation="LVITPRSDRWAAVTLFQGEQPVGMAGHPQVFSAAGEALLKFPET
                     TASWRRLDPDEKPEQLSAGPSGWEVVKPTDSIPLGPNQRIAESFYFKLEPGRWWLMLS
                     GNGLAFNPRIFVNGREAAARDYNQVDLAGLTSADTNTVTILYDTTAPARQNIKETRVA
                     LSLKLIRTEAVGLLGYVGHPQYAMLEGLRIFPRTTFFILDRNGRELLDRAVVDLSKLK
                     IEPARIQYASILNRSTAENGFCRPAGSRYRFDNGQPGWFWGGHENHPPTHATTDRYVE
                     SYRSMGVNVLRLISMRHELVRDYRDNSLDPVQLDKLHYLIYRLGQAGIYFHFTLSSYG
                     NYEGDTDPGGTEPGIWATAWDRGSMEKTGRSVTGEGQMTSPRIRELDKQLFRNLLTSK
                     NPYNGLRICDDPTVMTVELANEIGLGGRRFDWSIQYDPEVYKYQMHLWNQYLLKKYGP
                     REVYYQDYALGKLWKAEDYYKGEDPAADAVSWPQNFKGSRTRPVSYYRYYLDGVNRIA
                     NPRISDAMECLSRIQEDWFRDLYAYLKNECGVKVGIGASSDGFNIYQVGARLANARSL
                     DVTDGQAYGGAGQITRGFGGWGYLTDGFYVYNRPSFAREWGSWWTSSAQPWEFIVLAA
                     VLARYNGYDGFTHHKLGGLLYPVRDPRRYTINPCGDIRRSAFSLAAWIIQRSRIEEIE
                     PKIIIGFPEDDVFYGGANIPFSNHLGTGFLTGQLSTEEYYFKKEYDGPADRIVFHAGR
                     GPTGDYTKARHAILWCHGNSDRFAQDYNYKEKWFASHGIKFKEGESWYLDDHYLATTE
                     DLRGPNQEVFLGKLYEALKRWGHPLPFSPDEILKGWWSPDRKAAIEFEKMRMTVDRED
                     LQLYLGRFKGVGSHRLSRLSVASDEEQLSAACVPFDTADFRTSRQLLVWCLQNAAVTV
                     KLPFVGPAEVYAVNHLGSRIYRVRSTRPAADEIGFKTARSFDISYYEIVPAAGR"
BASE COUNT         1049 a         1925 c         2024 g         1097 t
ORIGIN      
        1 ggactgctcg accgggtcga gcacctggaa gactagcacc tcgtggtact tgtgacggaa
       61 atgaaccagc cctttcagga cgcgctccgg atcatccagg aggtcggaga tgagaatgat
      121 caggctgcgg cgccgggccc gggccgcgat ggtatgaagc acctcgccca ggttcgttgt
      181 ctcggcggcg gcggtcacct ggagctgctc caggaggtgg tgcaggtggc ggctggtgga
      241 ggccggcttg accatccggc ggaccgccga atcaaagacc atcaggccga ccgcgtcctt
      301 ctggttgacc atcaggtaac tgagcccggc ggccaggtag accgcgtact ccagcttgct
      361 caatccccgg ccgcggtagg ccatcgaacc gctggcgtcg agcaggatga ggctcttgag
      421 gttggtctcc tcccggaagg tcttcacgta gaggcggtcg gtccggccga agacgcgcca
      481 gtcgaggtaa cgcgggtcgt cgcccggcgc gtactcccgg tgctcggcaa actctacgct
      541 gctgcccttg tgcaggcttc ggtgcagtcc ggtaagcgac ccttccacca cggtccgggc
      601 caccaggtaa agattggaaa gccgggccag caccgccggg tcgagcaggc gacggtcttc
      661 ggccatgact cagtcgggga aggtttccag caggcgggtc acgatccagc tgctgtccag
      721 gccttcggcg tcggcgttga agttggtgaa gatccggtgg cgcagaacgt tcatggccac
      781 cgatcggata tcggcgacgc tcacgttgac gtccccgcgc agggcggcgg ccgccttggc
      841 gcccagcacc aggtactggg cggcccgcgg gccggcgccc cagctcacga aattgcggat
      901 gaactcgggc gcttcgccgt tgttgggccg gctctggcgg gtcagtttca ccgcgtaacc
      961 cagcacgtaa tccgagaccg gtatctcgcg gaccagcgac tgaaattcca ggatgcgccc
     1021 gcactccagc aggttcttca cctcggggtt caactcggaa acggtctgtt cggcgatgcg
     1081 cttctcctcc tcggcggccg ggtaatcgag atggacgctg aacataaagc ggtccagctg
     1141 cgcctcgggc agagggtagg tgccctcgag ttcgatcggg ttctgggtgg ccagcaccag
     1201 gaagggcagc ggcagccggt aagcgtttcc ggccgcggtc acctggtact cctgcatcgc
     1261 ctccagcagg gccgcctggg tcttgggcgt ggcccggtta atctcatcgg ccagcaccag
     1321 gttggcaaag accggcccgg gccggaagcg gaagttgcgg acccgcgaaa ccgggtcctc
     1381 ctggatcagc tcggtaccgg tgatgtccga gggcatcagg tccggagtga actggatccg
     1441 cttgaaactg aggtcaagga tcttggccag ggtccggacc atcagggtct tggccagtcc
     1501 cggcaccccg atcagcaggc agtggccccc ggcaaagagg ccggccatga tcttttcgat
     1561 gatctcgtcc tggccgatga tcacccggtg catctcccgt atcagccctt cgcgggtctt
     1621 cttcaagtct tcaagcaggc ggagtttctc gtccatgctc tcctttggcg gaagatgttc
     1681 aatgggtcaa gaggtaggcg atcaggttga cgcccagctg gccggactgc gaggccgaca
     1741 ggccggaggt gtaagggcgg gcgattccct cccagccgcc gcccagcccg tgggctgaaa
     1801 agaccgccgc cggcagcccg tcgagcaggt aaacctcgag cggcccctgg ttgcccgggg
     1861 tggcgtcggt gtaaagaaag gattccagcc ggtagggcgc ccggtaaacc gggtggcccg
     1921 tctcccagcg cgccggttca atgccgggca gcagctcggc catcagtttc cggaaggcgg
     1981 cgccgaattc ctggctgccg cagcagctgt ccgcgatcag ggtgccgccc cggcgcagga
     2041 aggcgcgcag tttttcgatc tccggccggc tcaggcgcgg atcgaagtgg ccggtaaggt
     2101 aaaggatcgg gatgtcgtcc agcggatcct tcttgagatc gacgttgatc cgttccaggt
     2161 agaccggcac cttggcctcc tcggccaccc ggtaaagaag ccggcccagg gcggccgggt
     2221 gcggatccca gtcgccggcg tgcatcacct ggccaaggta gatcttgccg ctcttcttgc
     2281 gggtcttctc ggcgtagacc ggcatctggc cgtacagttt cccgaactcg atccaggcca
     2341 ggcagtaact gaccaggttg acgccgatcc gaaccgcgtc ctcgggcagg tagcgccggc
     2401 cgaagtcgtg ggtgtgcagg gcccagccgc aacccaggtc gaggccggaa acaatcaccg
     2461 ccgcccggca gccgaggttg agcacccgca ggtccgggct gcccgaggcc ttgacgccgt
     2521 tctccatgat cccgaccttc tccaggttgt ggaaggcatg cttgagcggg tggttcgggt
     2581 aaagcgggta gaggttgcgg tcgggataaa tcttccccag ccagtcggcc agggaccgga
     2641 cgaactccgg gctgcccgag gaggcattgg cccagatgaa gccgcccttt tccaggtagt
     2701 tcttgaaccg cggcagcagg ttgtcgccgg gcgtgaaggg ttcaacgctg gtcaggtaga
     2761 ggatcggcac ttcctcgggg tcaaagctga aacgggccag gtcaatccgc accgcccggt
     2821 agggaacgct caactgctga gaggcgagcc ggagcaggtt gtccacgtca ttctcggccc
     2881 gggtccagcg gtcgtccggg ccgcagacca ccttgccgac caggaccggc ggcgagggcg
     2941 ggttcttctt ctcgctgcgc cgcagcgggg tggccggcag cggcagcggc ggcaccgcct
     3001 cgcccgcctt gcgcgtctgg ggcgtctgga ccggcggcgc cttgccgcat tcccaggccc
     3061 cggccggggt cgccgcgaaa accaaaagta gcaatgccgc cggcgcccaa cacttgattt
     3121 tacgcatctt acccagctcc tttccgcctt gcaatttaga gcggccggac ccggactccg
     3181 tttaccttac cggccggccg ccggaacgat ctcgtaatag gagatatcga agctccgggc
     3241 cgtcttgaaa ccgatctcgt cggccgccgg ccgggtggac ctgacccggt agatccggct
     3301 gcccaggtgg ttcaccgcgt agacctcggc cgggccgacg aagggaagct tgacggtcac
     3361 cgcggcattc tggaggcacc ataccagcag ctgccgggaa gtccggaaat cggcggtatc
     3421 gaagggcacg caggcggccg agagctgctc ttcgtcgctg gcaaccgaca gccgggaaag
     3481 ccggtggctc ccgacgccct tgaaacgccc caggtaaagc tggaggtctt cgcggtccac
     3541 ggtcatccgc atcttttcaa actcgatggc cgccttccgg tccgggctcc accaaccctt
     3601 gagtatctca tccgggctga aaggcagcgg gtggccccag cgtttcagcg cctcgtagag
     3661 cttcccgaga aagacttcct ggttcggccc gcgcaggtcc tcggtggtgg ccaggtaatg
     3721 gtcgtccagg taccagcttt ccccctcctt gaacttgatg ccgtggctgg cgaaccactt
     3781 ctccttgtaa ttgtaatcct gggcgaagcg gtcggaattc ccgtggcacc agaggatggc
     3841 gtggcgggcc ttcgtgtagt ccccggtcgg cccgcggccg gcgtgaaaga cgatccggtc
     3901 ggccgggccg tcatactcct tcttgaaata atactcctcg gtcgaaagct gcccggtcag
     3961 gaaaccggtg cccaggtgat tgctgaaggg aatgttggcg ccgccgtaaa aaacgtcatc
     4021 ctccgggaac ccgatgatga tcttgggctc gatctcctca atccgggaac gctggattat
     4081 ccaggcggcc agtgaaaagg cgctccggcg gatgtcgccg cagggattga tggtatagcg
     4141 ccggggatcc cggaccgggt agagcaggcc gccgagcttg tggtgggtga aaccgtcgta
     4201 gccgttgtag cgggccagga ccgcggccag cactatgaac tcccagggct gggcgctgct
     4261 ggtccaccag gaaccccact cccgggcgaa ggaaggacgg ttatagacgt agaaaccgtc
     4321 ggtaaggtat ccccagcccc cgaagccgcg cgtgatctgg ccggccccgc cgtaggcctg
     4381 gccgtcggtg acgtccaggg accgggcatt ggccagccgg gcacccacct ggtagatgtt
     4441 gaacccgtcc gaactggcgc cgatgccgac cttgaccccg cactcattct tgagataggc
     4501 gtacaggtcg cggaaccagt cctcctggat ccggctcagg cactccatgg cgtcggagat
     4561 gcgcgggttg gcgatccggt tcaccccgtc caggtagtag cgatagtagg agaccggccg
     4621 ggtgcggctg cccttgaaat tctggggcca gctgaccgcg tcggcggccg ggtcctcgcc
     4681 cttgtagtaa tcctcggcct tccagagttt gcccagcgcg taatcctggt agtagacctc
     4741 gcgcggaccg tacttcttga gcaggtactg gttccagagg tgcatctggt acttgtagac
     4801 ctccgggtcg tactggatcg accagtcgaa acgacgcccc cccagtccaa tctcgttggc
     4861 cagttccacc gtcatcaccg tcgggtcatc gcagatccgg agcccgttgt aagggttctt
     4921 cgaagtcagc aggttcctga agagctgctt gtccagctcc cggatccggg ggctggtcat
     4981 ctgcccctcg cccgtcacgc tgcggccggt cttctccatg ctgccccggt cccaggcggt
     5041 cgcccagata cccggttcgg tgccgcccgg atcggtatcc ccctcgtagt tgccgtagga
     5101 agagagggtg aaatggaagt agatgcccgc ctggccgagc cggtaaatca ggtagtggag
     5161 cttatccagc tggaccgggt cgagcgagtt gtcccggtaa tcccgcacca gttcgtggcg
     5221 catggagata aggcgcagga cgttgacacc catcgaacgg tagctttcaa cgtagcggtc
     5281 ggtcgtcgcg tgagtgggcg gatggttctc gtggccgccc cagaaccagc ccggctggcc
     5341 attgtcaaac cggtaccggc tgccggccgg ccggcagaag ccgttctcgg cggtgctccg
     5401 attgaggatc gaggcgtact gaatccgggc cggctcgatc ttcagtttcg acaggtcaac
     5461 cactgcccgg tcaagaagct cccggccgtt ccggtccagg atgaagaagg tggtgcgggg
     5521 aaagatccga aggccttcca gcatcgcgta ctgggggtgg ccgacatatc caaggaggcc
     5581 caccgcctcg gtccggatca gcttcaggga aagcgccacc cgggtctctt tgatattctg
     5641 gcgcgccggc gcggtggtgt cataaagaat ggtgacggtg ttggtgtcgg cgcttgtcag
     5701 gccggccagg tcaacctggt tgtaatcgcg cgccgccgcc tcccgaccgt tgacgaagat
     5761 ccgggggttg aaggcgaggc cattcccgga gagcatcaac caccaccggc ccggctccaa
     5821 cttaaagtaa aaactttccg ctatccgctg gttcggaccc agtggtatcg aatcggttgg
     5881 tttgacaacc tcccagccgg acggcccggc ggagagttgt tccggtttct cgtccggatc
     5941 aaggcggcgc caggaggcgg tcgtttccgg gaacttcagg agcgcctccc cggccgccga
     6001 aaagacctgc ggatgaccgg ccattccgac cggctgttcg ccctggaaga gggtgaccgc
     6061 cgcccagcgg tcggagcgcg gggtgatcac cagcc
//
LOCUS       DAOQYN010000115         5963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_13599_length_5963_cov_3.966994, whole genome shotgun sequence.
ACCESSION   DAOQYN010000115 DAOQYN010000000
VERSION     DAOQYN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13599_length_5963_cov_3.966994"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="PKN80_05600"
     CDS             complement(<1..93)
                     /locus_tag="PKN80_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNQ35523.1"
                     /translation="MLIIPAIDILDGRLVRLSQGDYDRVSDYRLD"
     gene            complement(71..1060)
                     /gene="lpxK"
                     /locus_tag="PKN80_05605"
     CDS             complement(71..1060)
                     /gene="lpxK"
                     /locus_tag="PKN80_05605"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNQ35524.1"
                     /translation="MLSLLVPLYYVLSESRRLWYRLGWRRPVRLPAYVVSVGNLTVGG
                     TGKTSVAGYLASFYARAGRRVAVVSHGYRGDRGGLVVSDGRGILARVERSGDDALLLA
                     RQLPGIPVLSGRRREELARRAVREFGAEVVILDDAFHYFRLARDLDILVFNTLAGLGN
                     GRLLPAGPLREPLRNIRRADLLWFNQADLVPESELKRFQSSLAVWAPAARRVTSSAVF
                     TCFSRLDGSDLPVSELSGMRVWVLAGIGAPDGFLQRLERLGLNQAGRSLYPDHHAYRK
                     EELLALPWNDFDCLVTTAKDLVRLPPGPWPRPVVYPKLELKVKGFPDADNSGH"
     gene            complement(1066..2289)
                     /locus_tag="PKN80_05610"
     CDS             complement(1066..2289)
                     /locus_tag="PKN80_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HNQ35525.1"
                     /translation="MFIADLHIHSKYSRATSRDMDLEHLAQSARQKGINLLGTGDFTH
                     PLWLAELKQKLQPAEPGLFLYAGLRFILTAEICNIFESRGSIKRIHNLVFLPALESAD
                     RLARKLGSYGSVTVDGRPMLQLSADDLAGIVRDIDPDGFLVPAHIWTPHFSLFGSNSG
                     FDRLADCFPKRHGEIFAVETGLSSDPEMNWRLSALDGLALGSNSDAHSPAKIGREVNV
                     FSQPLDYHQLRQVLAEKDDRRFLYTVEYFPEEGKYHYDGHRLCKTALAPEASRRLNNL
                     CPVCGRKVTVGVLHRVLDLADRPEGGRPERAIPFRRMIPLDQIIAFALGKTVDSPAVR
                     DSYQKIIRSLGTEFEILLTIPEADIRAQAEPRLADAVISVRAGRVKVEPGYDGEFGRI
                     EIFPEAAAPQQSTLF"
     gene            complement(2276..2794)
                     /locus_tag="PKN80_05615"
     CDS             complement(2276..2794)
                     /locus_tag="PKN80_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNQ35526.1"
                     /translation="MSRKPDPNEVIWSPWRKGYVIKPDKEAVCVFCRAAREVKHDRRN
                     LVLTRSGAGFVIINRFPYNNGHLMVVPNRHLSDLTELAADEAADLFELARRAVLVLKE
                     RLRPEGFNLGLNLGRAAGAGIASHLHIHIVPRWNGDTNFMPVVGGVKTVPDSLDALYR
                     ELRKDFQADVHR"
     gene            complement(2799..3623)
                     /locus_tag="PKN80_05620"
     CDS             complement(2799..3623)
                     /locus_tag="PKN80_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNQ35527.1"
                     /translation="MKYLRVRGRRTGPEGAFGRLDGKRVQLLDRPPFDGTAQPTGDWL
                     EWEEIEAYLPPAAPPNIFALGVNYARHGHEFKESLPTAPLIFLKATTSLAAHGDPILL
                     PGPAPDEVDYEAELVVVIGRRARNVPPEKALEHVFGYTCGNDVTARDCQMKYDRQWTR
                     AKSFDTFAPVGPVIETGLDPARLRIASRLNGQVMQEASTADLIFKVPELVSYLSRQFT
                     LLPQTLIMTGTPSGVGFARRPPVFLRPGDRIEIEIEGIGVLVNPVAAAASGAGETE"
     gene            3793..3863
                     /locus_tag="PKN80_05625"
     tRNA            3793..3863
                     /locus_tag="PKN80_05625"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3825..3827,aa:Gly,seq:tcc)
     gene            3929..4942
                     /locus_tag="PKN80_05630"
     CDS             3929..4942
                     /locus_tag="PKN80_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNQ35528.1"
                     /translation="MFRSRNLTFICALIALAALAGSIGWFRGRVSRKPRLYRASGLVM
                     GSFYTLEFSRPAGIPPEAAARHVRERLAELERRWSPYNPESEISRLNRTGRLAGVSPE
                     TRRLLELSAEISRRTGGAFDITVGPLIEAWRNAEKTGRPPDLKKLLPLVDYRRVRLTG
                     TGEVRLERKGMRIDPGAIGKGLAVDEAVRILRRDGVPSGLVNLGGNLYAFGPGPDRGL
                     WTIGIQDPFGPGEKGRLRIRDQGVSTSGTYQQWFQVGQKRFSHIVNPGNGELKSPPRS
                     VTVLAPGAGLADGLSTALEVLGPEAGLALLESIPGTSALIIMDDGRVIRSRGLDWPGA
                     GLP"
     gene            complement(4966..>5963)
                     /locus_tag="PKN80_05635"
     CDS             complement(4966..>5963)
                     /locus_tag="PKN80_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNQ35529.1"
                     /translation="IDDVLRNVVSGRIKPLAGRNGPSDAVVLKPLAASGRRGVAVANG
                     INLYGRLDPYRAAANAIDEALRNLTAVGGDPDRASLLDNFCWGDVGDPEVLGALVRAA
                     RACRDLSLAYRVPFISGKDSLNNYFVTDDGRHLSVPPILLVSSLSTVPNLERAVTLDL
                     KRAGSRLYLLGETFPELGGSHYLRRLGLEGGTVPEVRPVPARERMLGLHRAIMDGLVL
                     SCHDLSEGGLAVAAAEMAFSGGLGLELDLRRGGFKEMERDDQVLFSESASRFLVEVEP
                     DRAGDFEARLSGTHLAAFGRVVAEPHLSVIGLGGGEIIRSSLAELGRAWRDALPW"
BASE COUNT         1088 a         1971 c         1958 g          946 t
ORIGIN      
        1 gtccagccgg taatcggaga cgcggtcata atctccctgg gagaggcgga ccaggcgtcc
       61 gtccaggatg tcaatggccg gaattatcag catcggggaa tcctttcact ttcagctcca
      121 acttcggata aacgaccggc ctcggccagg ggcccggcgg caggcgcacc aggtccttgg
      181 cggtggtcac caggcaatcg aaatcattcc agggaagggc cagcagctcc tccttccggt
      241 aggcgtgatg atcagggtag aggctccggc ccgcctggtt cagcccgagc cgttccagcc
      301 gctggaggaa accgtccggg gccccgattc cggccaggac ccagacccgc attccggaga
      361 gttccgaaac cgggagatcg cttccatcca accggctgaa acaggtgaag acggccgaac
      421 tggtgacgcg gcgcgcggcg ggggcccaaa cggccaggga ggactggaac cgtttcagtt
      481 ccgactccgg aaccaggtcg gcctggttga accagagcag gtcggcccgc cggatgttcc
      541 gcagcggctc ccgaaggggg ccggccggca gcaaacggcc gttgccaaga ccggccagcg
      601 tgttgaaaac cagtatgtcg agatcccggg ccagccggaa atagtgaaaa gcgtcgtcca
      661 ggatcaccac ctcggcgccg aattcccgga ccgcccggcg ggccagctcc tcccggcggc
      721 ggccggagag caccgggatc cccggtaatt gcctggccag gagcagggcg tcgtcgccgg
      781 accgttcaac ccgggccaga attccccgcc cgtccgaaac gaccagcccg ccccggtcac
      841 cacgatagcc gtggctgacc accgccaccc ggcggcccgc ccgcgcataa aaagaggcca
      901 ggtatccggc cacgctggtc ttgccggtcc cccccaccgt gaggttgccc acgctgacca
      961 cgtaggccgg cagccggacc ggccgtcgcc agccaagccg gtaccagagg cggcgtgact
     1021 ccgatagaac atagtaaagg ggaacgagga gggacagcat ccggttcaaa agagagtcga
     1081 ctgctgcggc gcggccgctt ccgggaaaat ttcgatccgg ccgaactccc cgtcatagcc
     1141 gggctccacc ttaacccggc cggcccggac cgagatgacg gcgtccgcca gccggggttc
     1201 agcctgggcc cggatatcgg cctccggaat ggtcaggaga atttcaaact cggttcccag
     1261 ggaccggatg atcttctggt agctgtcccg caccgccggg ctgtccaccg tttttccaag
     1321 cgcaaaggcg ataatctggt ccagggggat catccggcgg aagggtatgg cacgttccgg
     1381 acggccgcct tccggccggt cggccaggtc cagcacccgg tgcagaaccc cgaccgtaac
     1441 cttccgtccg caaaccgggc agagattgtt tagccggcgc gaagcctcgg gcgcgagcgc
     1501 cgtcttgcac aggcggtgac cgtcatagtg gtactttccc tcctccggaa aatactcgac
     1561 ggtataaagg aaacggcggt catccttttc ggcgagaacc tgccggagct ggtggtaatc
     1621 gagcggctgg ctgaaaacat tgacctcccg gccgatcttg gccggagagt gggcatcgga
     1681 attggaaccc agggccaggc catcgagggc cgacaggcgc cagttcattt ccgggtcgct
     1741 cgaaagcccg gtctccaccg cgaagatttc gccgtgccgc ttcggaaagc agtcggccag
     1801 ccggtcaaag ccgctgttgg aaccgaagag cgaaaaatga ggtgtccaga tgtgggccgg
     1861 caccaggaaa ccatccggat cgatatccct gacgatcccg gccaggtcgt cggcggaaag
     1921 ctggagcatc ggccggccgt cgacggtgac gctcccgtag gaaccaagct tgcgggcaag
     1981 ccggtcggcc gattccagcg cgggcaggaa gaccaggttg tggatccgct tgatgctgcc
     2041 ccggctttcg aagatgttgc atatttcagc ggtcaggatg aacctcaggc cggcgtaaag
     2101 aaagaggccc ggctcggccg gctggagctt ctgcttcaat tcggccagcc ataacgggtg
     2161 ggtgaaatca ccggtgccga gcaggttgat acccttctgg cgggccgatt gggccaggtg
     2221 ttccaggtcc atgtcgcggc tggtggcacg cgaatacttt gaatggatgt gtaaatcagc
     2281 gatgaacatc ggcctggaaa tcctttctga gttcccggta gagggcatcc agcgaatcgg
     2341 ggaccgtctt caccccgccc acgacgggca tgaaattggt gtctccattc cagcggggaa
     2401 cgatgtggat gtgcaggtgc gaggcgatgc ccgccccggc ggcccgcccc aggttcagcc
     2461 cgagattgaa cccttccggc cggagccgtt ccttgaggac cagcacggcg cggcgggcca
     2521 gttcgaaaag atcggccgct tcgtcggccg ccagttcggt caggtcgctc aggtgccggt
     2581 tgggaactac catcaggtgt ccgttgttgt aggggaagcg gttgatgatc acaaaacccg
     2641 cgccgctcct ggtcaggacc aggttccggc ggtcatgttt aacctcccgc gcggcccggc
     2701 agaaaacgca gaccgcctcc ttgtccggct tgataacgta tcccttgcgc cagggggacc
     2761 agattacctc gttgggatcg ggtttccggc tcatgggctc attcggtttc accggcgccg
     2821 gaggccgcgg cggccaccgg gttgaccagg acgccgatgc cttcgatctc gatctcgatc
     2881 cggtcacccg gacgcaggaa aaccggcggc cggcgcgcga aaccgacccc ggaaggcgtg
     2941 ccggtcatga tcaaagtctg gggaagcagg gtaaactggc gggaaaggta actgaccagc
     3001 tccggaacct tgaagatcag gtcggcggtg ctggcctcct gcatgacctg tccgttgagc
     3061 cgggaggcta tccggaggcg ggccggatcc agcccggttt cgatgaccgg tccaaccggc
     3121 gcgaaggtat cgaaagactt ggcccgggtc cactgacggt cgtacttcat ctggcagtcc
     3181 ctggccgtga catcgtttcc gcaggtgtaa ccgaaaacat gctcaagggc cttttccggc
     3241 ggcacgttgc gggccctccg gcctatcacc accaccagct cggcctcgta atcaacctcg
     3301 tccggcgccg ggcccggcag cagaatcggg tccccgtggg cggccaggct ggtggtcgcc
     3361 ttaaggaaga tcagcggcgc cgttggcagg gactccttga attcatgccc gtgccgggcg
     3421 taattcaccc ccagggcgaa gatgttgggg ggggccgccg gcggcaggta ggcctcgatc
     3481 tcctcccatt caagccagtc gccggtcggc tgggccgttc cgtcgaatgg cggccggtca
     3541 agcagctgca ccctttttcc atcaagccgt ccgaaggcgc cttccggccc ggtccggcgg
     3601 cctcgaaccc gcaggtattt catgctttga ttttacaata tttggaggcc ggcatcaaga
     3661 ccggaaaggg ctcccggcct tgacagcggc gctcaaaaag ccgccggggc ggcgcccgcc
     3721 gccgcccccc ggaaccttga caggccgcgg ccggtcggag tatcttaaat aatccgaggt
     3781 tttcaaatcc acgcggggtt agtttagtgg taaaaccaca gccttccaag ctgttgatgg
     3841 gggttcgatt cccctacccc gctcgaggcg gcccggtccg gcggcggccg gcgccgccgg
     3901 cctttcaacc ccacccgtta aattcgcaat gttccggtcc cgaaacctga ctttcatttg
     3961 cgccctgatc gccctggcgg ccctggccgg atcgatcggc tggttccggg ggcgcgtctc
     4021 ccgaaagccc cgtctttacc gggcctccgg cctggtgatg ggttcctttt acaccctgga
     4081 attcagccgg ccggccggga tacctcccga ggcggcggcc cgccatgtcc gggaacgcct
     4141 tgcggaactg gaacgccgct ggtctcccta caacccggag agtgaaatca gccgcttgaa
     4201 tcggaccgga cggctggccg gcgtcagccc ggagacccgg cgtctcctgg aattgtccgc
     4261 cgagatttcc cggcgtaccg gcggcgcctt cgacatcacg gtcggccccc tgatcgaggc
     4321 ctggcgcaac gccgaaaaga ccgggcggcc gcccgatttg aaaaaactgc tgcccctggt
     4381 ggactaccgg cgggtgcggt taaccgggac cggggaagtg cggctggaaa gaaagggcat
     4441 gcgcatcgat cccggcgcca tcggcaaggg gctggcggtg gacgaggcgg tccgaatcct
     4501 gcgaagggac ggcgtgccgt cgggcctggt aaacctgggc ggcaatcttt acgccttcgg
     4561 ccccggaccc gaccggggcc tctggacgat cggcatccag gatcccttcg gccccggaga
     4621 aaaaggaagg ctgcggatcc gggaccaggg agtttccacc agcggcacct accagcagtg
     4681 gttccaggtg gggcagaagc gtttcagcca catcgtcaac ccggggaacg gcgagttgaa
     4741 atcgcccccg cgcagcgtca cggtcctggc ccccggagcc ggactggccg acggactctc
     4801 caccgcgctg gaggtgctcg gacccgaggc cggactcgcg ctgctggaaa gcatcccggg
     4861 gaccagcgcc ctgatcatca tggatgacgg ccgggtgatc cgctccaggg ggctggactg
     4921 gcccggggcc ggattgccct gaaggcggct gatccgggcg tcggctcacc agggcagggc
     4981 gtcccgccag gcccggccca gctccgccag gctcgagcgg atgatttccc cgccgcccag
     5041 tccaatcacc gacaggtgcg gttcagcgac aacccgcccg aaggcggcca ggtgagtacc
     5101 ggagaggcgg gcttcgaaat cacccgcccg gtccggttcc acttcaacca ggaaacggct
     5161 ggccgactcg gaaaagagca cctggtcatc gcgctccatc tccttgaagc cgccccgccg
     5221 cagatcaagc tccaatccca gcccgccgct gaaggccatc tcggccgccg ccaccgccag
     5281 gccgccctcg gaaagatcgt ggcaggagag caccaggccg tccatgatgg cccggtgcag
     5341 ccccagcatc cgctcccggg ccgggaccgg ccggacctcc ggaaccgtgc cgccctcgag
     5401 accgagccgg cgcaggtaat gggaaccgcc cagctccggg aacgtctcgc ccagcaggta
     5461 gaggcgcgaa ccggcccgct tgaggtccag ggtgaccgcc cgttccaggt tgggaaccgt
     5521 cgaaagcgag gagaccagca ggatcggcgg caccgagagg tggcggccgt catcggtgac
     5581 aaagtaattg ttcaggctgt ccttgccgga gatgaaggga acccggtagg ccaggctcag
     5641 gtcgcggcag gcccgggccg cgcgcaccag ggcgcccagg acctccgggt cgccgacatc
     5701 cccccagcag aagttgtcca gcaggctggc ccggtccgga tcgccgccca ccgccgtgag
     5761 attacgcagg gcttcatcga tggcgttggc cgccgcccgg tagggatcca ggcgtccgta
     5821 aagattgatt ccgttggcaa ccgcgacgcc ccgccggccg gaagcggcca gcggcttgag
     5881 aacgaccgca tccgaaggac cattccggcc ggccagcggc ttgatccgcc ccgacaccac
     5941 gttgcgcagc acgtcgtcga tcg
//
LOCUS       DAOQYN010000116         5917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_13756_length_5917_cov_4.856022, whole genome shotgun sequence.
ACCESSION   DAOQYN010000116 DAOQYN010000000
VERSION     DAOQYN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13756_length_5917_cov_4.856022"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PKN80_05640"
     CDS             complement(<1..799)
                     /locus_tag="PKN80_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNQ35530.1"
                     /translation="MKSFRKNVFRLNRYIRIPWRMMTATVILMIVNSALAGISITAIG
                     PILDVVFNHGTLHLPASLPAGLRPGLEQLAGVVNRMPPLLLLSRILAILTAAFLVKAV
                     IGYFQQYLNGKFALKIVAQLRREVFGRYLNSPLELNQRKRLGERLSHFTYDIYLLQTA
                     FGTNLPKLVFNFILTAAYFFVILFIHWRLTLLSLLILPAFAWPTLRFGQRIRKLSRDS
                     QESLANLNSLIQESLTNLPVVKSFNAQPRVEERFEAENFRFYRVSLKS"
     gene            complement(796..1905)
                     /gene="lpxB"
                     /locus_tag="PKN80_05645"
     CDS             complement(796..1905)
                     /gene="lpxB"
                     /locus_tag="PKN80_05645"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNQ35531.1"
                     /translation="MKIFISTADHSGDFHASRLAAALKEAAPGLNLCGVGGPLMEAAG
                     VKLAARPAEKAVVGFFEVVRSLPAWRDFLDRAAAAGRREKPDLTVLVDSPSFNIRLAR
                     RLAGNGGRFVYYISPQVWAWGAGRVREIARLCREVLVIFPFETEIYRRAGVPVRFVGH
                     PFLDEVRADRPAAETRRRLGLDPDRPLLLLLPGSRESEVRRLLPVLAATAAAAREKID
                     LQTVLYPARPGLLDGWRPPAGIRVLPEGDKYSVFAAADLALAASGSVTLELTLLGVPF
                     ILLYRLAAPTYWMLRPMLRVRQAGITNILAGRTVAPEFLQGRCRPELILPETLALLGD
                     PARREEMKRQFLKVRESLGEPGAARRAARRILELA"
     gene            complement(1958..2467)
                     /locus_tag="PKN80_05650"
     CDS             complement(1958..2467)
                     /locus_tag="PKN80_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35532.1"
                     /translation="MAKIDRRVPVLGMVLAYILAVGLVAAPNWREARRLERERAAAQH
                     SLDNLLEINARYQRSSSGLKGALRPDGATGLLTVAQQTARQLNISEKLKSAVPVYREL
                     TGGLQAEGFDLRLEGLNTEEAVRLAEALQREDGFYLDRLALERSRDGLSLSARLRFLA
                     LKKEAGPAR"
     gene            complement(2478..3719)
                     /locus_tag="PKN80_05655"
     CDS             complement(2478..3719)
                     /locus_tag="PKN80_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35533.1"
                     /translation="MKKVAGFFTGSGFSRRTTLYLEETRAWLWAGGGPFQKAGDAVSF
                     EADRLERLLEELKNGFQARPGERVTVILAPELVLEKAVFLPFRQARQVALVLPEELAA
                     VLPDSLEEYRYGFLAPPDDPFPVTAFLAREADLSRIAAALQPGRPRFVTLEPVLASLK
                     AAGRDQVILLIRGGRSHRLALFQKGRFAMGRSFRSGPAEFEERLREERELLLRSARLP
                     ETVESVEFNPARELDPDRAGRTRAFPLLPGGGGQNRDRRLAGGLAMTAALLVLAALTI
                     FSTQARRRAEADYRRVDNELRRQKTLAAGADANFSQLMADETAARLVRSDLSPLESFN
                     RFTVAVPENLPLTLEYLEIDRTRVLVNAALPEAGQLDRLVKALTRSGDFVNPVLGELK
                     VDKGLVRFPLTLGLKEAGPGR"
     gene            complement(3655..4659)
                     /gene="gspK"
                     /locus_tag="PKN80_05660"
     CDS             complement(3655..4659)
                     /gene="gspK"
                     /locus_tag="PKN80_05660"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HNQ35534.1"
                     /translation="MTLILIAILALAAVELFLFLRPKLSVSRELLQRTLLVNAASTGI
                     DYARSLLLEDLENSEFDSLEEPWNEPVEYALDRVEVKVRIEDEERRFPLAFLIVEPAA
                     AAGGQPSPAAGTPAAKPAVGTAPAESGRTDTGIKLSEVGLEAFETLLKRLGLTPNWAK
                     EAVDSLADWIDGDDDPRSFGAEKDYYAVNGYQARNGLPDNLGELALVKGFRPDITEKV
                     FPYLTVFSKRLNLNTAPAEVLESMFGKDESLPIDFLVNGRPYESTADALKLAQIDPKF
                     YNQLQKMLTVSSSVFSVRSTARDESGRQESVYAIIERNEKGGRILYWQRLQPPDDPLP
                     "
     gene            complement(4616..5224)
                     /locus_tag="PKN80_05665"
     CDS             complement(4616..5224)
                     /locus_tag="PKN80_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ35535.1"
                     /translation="MRKRDRAGFTLIEILITLSLVVIIIGVLFASYFMPLRAIQQLRL
                     RLGREEAVAVFLNRLNGELESVFPREESFRGDETALSFLTAASGDLALERISYQQVES
                     GEKSVLVYRKADPSFRLETGADTGVWPPPVEGEPVFQAERIRFSYYDGEEWQESWEKK
                     RPPRLVRVELERRGKEYRLLAPLRASLDSDTDLDSDTGLGGG"
     gene            complement(5221..5628)
                     /locus_tag="PKN80_05670"
     CDS             complement(5221..5628)
                     /locus_tag="PKN80_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ35536.1"
                     /translation="MPTSRTGKKTNKRGFTLIELVVSVLIMATGLLLLLVSIRQGLQT
                     GQTALRDLEDSIQAGNAAGAALAGLETTEEESDTEAWEASVEEPRPDLKFTRIAPARR
                     ETGPVVWISTLEPVEKETGAGEGTGTRTESPKP"
     gene            complement(5594..>5917)
                     /gene="gspG"
                     /locus_tag="PKN80_05675"
     CDS             complement(5594..>5917)
                     /gene="gspG"
                     /locus_tag="PKN80_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNQ35537.1"
                     /translation="EAKITTSRVQIQNLETALRLFYLDNGFYPTTEQGLQALVEAPGD
                     AKHWREGGYLEKKQVPKDGWGNPYLYRAPGEGGREYDILSLGRDGKEGGTGIDADLKN
                     WEENE"
BASE COUNT          971 a         1913 c         2008 g         1025 t
ORIGIN      
        1 cgctcttgag cgagacgcgg tagaagcgga agttttccgc ctcgaagcgc tcctcaaccc
       61 gcggctgggc attgaaggac ttgacgaccg gcaggttggt caggctctcc tggatgaggc
      121 tgttgaggtt ggccagcgac tcctggctgt cccggctcaa cttgcggatc cgctgtccga
      181 agcgcagagt cggccaggcg aaggcgggca ggatcagtag cgagagaagc gtcaggcgcc
      241 agtggatgaa gagaatgaca aagaagtagg cggcggtcag gatgaagttg aagaccagtt
      301 tcggcaggtt ggtgccgaag gcggtctgga gcaggtagat gtcataggtg aagtgggaga
      361 gacgctcgcc cagccgcttg cgctggttga gttccagggg ggagttgaga tagcggccga
      421 agacctcccg gcgcagctgg gccactattt tcaaggcgaa tttcccgttc aggtactgct
      481 ggaagtagcc gatgaccgcc ttgaccagga aggcggcggt caggatggcc aggatgcggc
      541 tgagaagcag cagcggcggc atccggttga cgaccccggc cagttgttcc aggccggggc
      601 gcaggccggc cggcaggctg gcgggaaggt ggagagtacc gtggttgaag acgacatcca
      661 ggatggggcc gatggcggtg atcgagatgc cggccagggc cgagttgacg atcatcagta
      721 tcaccgtggc cgtcatcatc cgccagggaa tccggatgta gcgattgaga cggaagacgt
      781 tctttcggaa acttttcatg ccagttccag tatgcgcctg gccgcccgcc gggccgctcc
      841 cggttcgccg agggattcgc gcaccttgag aaattgacgt ttcatctcct cccggcgcgc
      901 cgggtccccg agcagggcca gcgtctccgg caggataagt tcgggccggc agcggccctg
      961 gagaaactcc ggcgccaccg tccggccggc caagatgttg gtgatgccgg cctggcgcac
     1021 ccgtagcatc ggccgcagca tccagtaggt cggcgccgcc agccggtaaa gaaggatgaa
     1081 gggtacgccc agcagggtca gttccagggt cacgcttccg gaggcggcca gcgccaggtc
     1141 ggcggcggcg aagaccgaat atttgtcgcc ttcggggagc acccggattc cggccggcgg
     1201 ccgccagccg tccagcaggc cgggccgggc cgggtagagc accgtttgca ggtcaatctt
     1261 ttcccgggcg gcggccgccg tcgccgccag caccggcagc aggcgccgca cctccgattc
     1321 ccggctgccg ggcaggagca ggagcagcgg ccggtccggg tccagcccga ggcggcggcg
     1381 ggtctccgcc gccggccggt cagcccggac ctcgtccagg aaggggtggc cgacgaagcg
     1441 gaccggcacg ccggcccgcc ggtaaatctc ggtttcgaag ggaaaaatga ccagcacttc
     1501 gcggcagagc cgggcgattt cccgcacccg gcccgccccc caggcccaga cctgcgggct
     1561 gatgtaataa acgaaccggc cgccgttccc ggctaggcgc cgggccagcc ggatgttaaa
     1621 gctgggcgaa tcgaccagca ccgtaaggtc gggcttttcc cgccgtccgg cggccgcggc
     1681 ccgatccaga aaatcgcgcc aggccggcag gctccggacg acctcaaaga atccgaccac
     1741 cgccttttcg gccggccggg ccgccaattt gacgccggcc gcctccatca gcggcccgcc
     1801 cactccgcag agattgaggc cgggcgcggc ctccttgagg gcggcggcca gccggctggc
     1861 gtgaaagtcg ccggagtggt cggcggttga aatgaatatc ttcatcggcc tcgcaggggc
     1921 ggtcttcttc cgtcgcccgg tcttttcccg gaagcggtca gcgcgcgggg ccggcctctt
     1981 tcttgagggc caggaagcgg agccgggctg agagggagag gccgtcccgg cttcgttcga
     2041 gcgccagccg gtccaggtag aaaccgtcct cccgctggag cgcctcggcc aggcgcacgg
     2101 cctcctcggt gttgagtcct tccaggcgaa ggtcgaatcc ctcggcctgc aggccgccgg
     2161 tgagttcgcg gtagaccggt accgccgatt tgagtttttc ggagatgttg agctgccggg
     2221 cggtctgctg ggctacggtg agcaggccgg tggccccgtc cgggcgcagt gcccctttca
     2281 gaccggagct ggaccgctgg taacgggcat tgatctcgag caggttgtcc agggagtgct
     2341 gggcggcggc ccgttcccgc tccagccgcc gggcctcgcg ccagttgggc gcggccacca
     2401 ggccgacggc cagaatgtag gccagcacca tgcccaggac cggcacccgc ctgtctattt
     2461 tggccatgat cttaccgtta ccggcccggg ccggcctcct tgagtcccag ggtgagcgga
     2521 aaacgaacca gtcccttatc caccttcagt tcgcccagga ccgggttgac gaaatcgccg
     2581 gaacgggtca gcgccttgac cagccggtcc agctggccgg cctccggcaa ggcggcgttg
     2641 acgagtaccc gggtccggtc tatctccagg tattcgagcg tcaggggcag gttttccggg
     2701 accgcgacgg taaagcggtt gaaactttca agcggcgaca ggtccgagcg caccagccgg
     2761 gcggccgttt cgtcggccat cagttgcgag aagttcgcgt cggcgccggc ggccagcgtc
     2821 ttctggcggc gcagctcatt gtccaccctg cggtagtcgg cctcggcccg gcggcgggcc
     2881 tgggtggaga agatggtcag cgcggccagc accagcaggg ccgctgtcat ggccaggccg
     2941 ccggccagac gccggtcccg gttctggccg ccaccgccgg gcaggagcgg gaaggcccgc
     3001 gtccggccgg cccggtccgg atcaagctcc cgggccgggt tgaattcgac gctctccacc
     3061 gtttccggga ggcgggccga tcggagcagg agttcccgtt cttcccgtag gcgctcttca
     3121 aattcggccg ggccggaccg gaagctccgg cccatcgcga agcgtccctt ctggaagagg
     3181 gccagccggt gggagcggcc gccccggatc aggagaatga cctggtcgcg gccggcggcc
     3241 ttcagggaag cgagcaccgg ttccagcgta acgaagcggg gccgcccggg ttgaagtgcc
     3301 gcggctattc tgctcaagtc cgcctcgcgc gccaggaagg cggtcaccgg gaacgggtcg
     3361 tccggcggcg ccaggaagcc gtagcgatac tcctccaggg aatcgggcag gaccgcggcc
     3421 agttcctcgg gcaggaccag ggccacctgg cgcgcctgcc ggaagggaag gaagacggcc
     3481 ttttccagga ccagttccgg ggcgagaatg accgtaaccc gctcccccgg ccgcgcctgg
     3541 aatccgttct tcagctcttc cagcagccgc tccaggcggt cggcctcgaa ggagacggcg
     3601 tcgccggcct tttgaaaggg tccgccgccg gcccagagcc aggcccgggt ctcctcaagg
     3661 tagagggtcg tccggcggct gaagccgctg ccagtaaaga atccggccac ctttttcatt
     3721 gcgttcgatt atggcgtaaa cgctctcctg ccgcccggat tcgtcccggg cggtcgatcg
     3781 caccgagaag accgagctgc tgacggtgag cattttctgt aattgattat agaattttgg
     3841 atcaatctgg gcaagtttca gggcgtcggc cgtcgattcg tagggtcggc cgttgaccag
     3901 aaaatcgatc ggcagggatt catccttgcc gaacatggac tcaagcactt ccgccggcgc
     3961 ggtgttgagg ttcagccgtt tcgagaagac ggtcaggtac ggaaagacct tttcggtgat
     4021 atccggacgg aatcccttga ccagggccag ttcgcccagg ttgtccggca gcccgttgcg
     4081 cgcctggtag ccgttgacgg cgtaataatc cttttcggcg ccgaaggaac gcggatcgtc
     4141 atcgccgtca atccagtcgg ccagcgagtc gacggcctcc ttggcccagt tgggtgtcag
     4201 tcccagccgc ttcagaagcg tttcgaaggc ctccaggccg acctcgctca gcttgattcc
     4261 ggtatcggtc cggccgcttt cggccggcgc cgtaccgacg gccggcttgg cggcgggcgt
     4321 cccggccgcc ggcgacggct ggccgccggc cgccgcggcc ggttcaacaa tcaggaaggc
     4381 cagcgggaag cgccgttcct cgtcctcgat ccggaccttc acctcgaccc ggtcaagggc
     4441 gtactcgacc ggttcattcc agggctcctc gagcgaatcg aattcgctgt tttcgaggtc
     4501 ttcgagcagc aggctgcggg cgtaatcgat gccggtcgag gcggcattga ccaggagcgt
     4561 ccgctgcagc aattcgcgcg agacggaaag tttcggccgg agaaagagga agagttcaac
     4621 cgccgccaag gccagtatcg ctatcaagat cagtgtcact atcaagactt gcccggagcg
     4681 gcgccagcag gcggtattcc ttgccccgcc gttccagctc gaccctgacc agccggggcg
     4741 gtcttttctt ctcccaggat tcctgccatt cttcgccatc atagtagctg aaccgtatcc
     4801 tttcggcctg gaagaccggt tctccctcga ccggcggcgg ccagacaccg gtatcggcgc
     4861 cggtctccag tcggaacgaa gggtcggcct tccggtaaac cagcacgctc ttttctcccg
     4921 attcgacctg ctggtaactg attctctcca gggccaggtc gccggaggcg gccgtcagga
     4981 aagaaagcgc ggtctcgtcg ccccggaagg attcctcgcg cggaaagacc gactccagct
     5041 ccccgttcag acggttgagg aagaccgcga ccgcctcctc ccggccgagc cgcaggcgca
     5101 gctgctggat ggcccgcaac ggcatgaagt aactggcgaa aagcaccccg atgatgatca
     5161 ccaccaggga aagggtgatc aggatttcga tcagggtgaa gcccgcccgg tcccgctttc
     5221 tcatggcttc gggctttcgg tccgggttcc ggttccctcc ccggccccgg tctctttctc
     5281 gacgggttcc agggtgctta tccatacgac cggtccggtt tccctgcgcg ccggcgcgat
     5341 ccgggtgaac ttgaggtccg gccggggctc ttcgacgctg gcctcccagg cttccgtatc
     5401 cgattcctct tcggttgtct ccaggccggc cagggcggcc ccggcggcat tgccggcctg
     5461 aatggagtcc tccaggtcgc ggagggccgt ctggcccgtc tggagcccct ggcggatcga
     5521 aaccagcagg aggagcaggc cggtggccat gattaacacg ctcaccacca gttcgatcag
     5581 ggtgaagccg cgcttattcg ttttcttccc agttcttgag gtcggcatcg attccggttc
     5641 ccccctcctt gccgtcccgg ccgagggaga gtatgtcata ctcgcgcccg ccttcgcccg
     5701 gcgcccggta gaggtaggga ttgccccagc cgtccttggg aacctgcttc ttctcgaggt
     5761 agccgccctc gcgccagtgc ttggcgtcac cgggcgcctc aaccagcgcc tggagcccct
     5821 gctcggtggt gggatagaag ccgttgtcca ggtaaaagag gcgcaacgcg gtttccaggt
     5881 tctggatctg gacccgcgag gtggttatct tggcctc
//
LOCUS       DAOQYN010000117         5899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_13797_length_5899_cov_5.030287, whole genome shotgun sequence.
ACCESSION   DAOQYN010000117 DAOQYN010000000
VERSION     DAOQYN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13797_length_5899_cov_5.030287"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /gene="lnt"
                     /locus_tag="PKN80_05680"
     CDS             complement(<1..1285)
                     /gene="lnt"
                     /locus_tag="PKN80_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNQ35538.1"
                     /translation="MKRLSILVAAFIAGVFFLLAYPPFNQGYLAWFGLLPILFLLRRG
                     RSFWSGYVWGMTVFGLGLLWLVRLSGLGWFAATLYLAIYPGLFFYLASRARGRLRDLW
                     AAALWVVLEFILGHLFTGLPWLNLAITQHWNPLIQPLLSFGGGPLVAFLVVLANFSLF
                     SFFSRPSGRRSAGWPGLLVVLAAFLAGQALSFRPPVPPPDAPILRFAVVQGNVPSSLR
                     RQDPPLEPYLYVTRQLSSPVDLIIWPETTLVSGSIERPPLAVRRLLREKRAHLLCGVL
                     EVDSAGRAYNSAALYAPGGRLLAVYRKRHLVPFGEYTPGRNLPPVRWLVERQAGFLPG
                     LSAGDLPAGIRLPKGSGLVPLTLYICFEDIFAEAAADTQRRHPGLMITLTNDSWLGRL
                     GAYQHLAAAALRAAENRTPLLRAANTGVSAWFDARG"
     gene            complement(1282..2700)
                     /locus_tag="PKN80_05685"
     CDS             complement(1282..2700)
                     /locus_tag="PKN80_05685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ35539.1"
                     /translation="MRLGQQAREKATGYAATRTVKAILALMPRISNTTLIRLTHLAEL
                     LVADTAQKERVRVIREKFERNHPSLEAAKRIARRLSVPAREKLIHNLVVNGMLLGTRR
                     RNSIRESEGWRPPFFIVISPTMRCNLNCIGCYAGSYERQDVLASADLDRILNQARELG
                     IYFVTISGGEPFMREDLLDLMQRHNDIYFQVYTNGTLITDRVADRLAALGNVAPAISV
                     EGYQAETDHRRGTGVYRKLEQVWKKLVEKGVMFGFSATPTRHNLELLFSDGFIDSMIE
                     AGALFGWYFHYIPIGSRPDVNLMPTPEQRAYARERVNDLRNRKPIILADFWNDGHLTG
                     GCIAGGRTYLHINANGDVEPCVFVHFAIDNIRERSLREAINSPLMRAIQKRQPYSPNL
                     MTPCMIIDQPWVLREVVRETGAHDTDHGGRCLLHEINGYLDNYSRSIHGIFDPIWDRE
                     YGRGRNLRIKYDHQPPSSGAPA"
     gene            complement(2715..3071)
                     /locus_tag="PKN80_05690"
     CDS             complement(2715..3071)
                     /locus_tag="PKN80_05690"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNQ35540.1"
                     /translation="MLRQFCKSKIQGLVVTGTDLNYPGSITIDSALAEKADLLPFEMV
                     QVLNLANGARIETYYIPGEPGRGQVCLNGAAARWFEAGDRVIVLSTAFYSAEELRGFQ
                     PRILLTDQENRIKEIR"
     gene            3293..5050
                     /locus_tag="PKN80_05695"
     CDS             3293..5050
                     /locus_tag="PKN80_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNQ35541.1"
                     /translation="MNLWLVILLTALLFHLAHRLYSRGLARVLGEEPERPTPAVTRND
                     GYDYVPSKTLVVFAHHFASIAGAGPIIGPTLALLYGFVPVYLWIIIGGIFIGAAHDFS
                     AVFISLRNQGRSIAEIARGQLGNTGYFLFVLFAIMVLTLVNAAFLNLSVTALTSQAPL
                     AAFGLGPGQHLLRVIQDSGGQIYGFIGGIASTSVIFITLLAPLVGWLLYRRGLNPVAG
                     SFLALAICLAAVAFGLAYPVSVPPAWWRVILTIYVFAAAGIPVWLLLQPRDFTNVQIL
                     YLGIGLMLMGILAAGFQGAPMAVPAFNVQAAQAKPALGQLWPFLFITVACGAISGFHA
                     LVAGGTTAKQLSREGAARTVGFDAMLLESIMAVAVIITLSCGLDFSHYQAVVFPAPES
                     GLQSNPILAFALALGGLLNRGFGMPTAVGTIFGILMLEGFIITTLDTSIRLQRYLFEE
                     VWGIIFQKPAGWLKSYYFNAGLAALVMFLLARGNAYTIIWPVFGAANQLLAALTLITV
                     SVWLTRRGRGRALFLVPAIFMLVTTIAALWLTLVRKFLPGRNYPLIAAALFLMLLAVC
                     VAGLAAGRLIRKEPADSRT"
     gene            complement(5069..5320)
                     /locus_tag="PKN80_05700"
     CDS             complement(5069..5320)
                     /locus_tag="PKN80_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNQ35542.1"
                     /translation="MREKIEKALDEVREFLRMEGGDVELVEISDDGRVKVRLMGACGH
                     CPFAALTLKMRVEKLVKERVPEVKSVESVDTDEPSGPAA"
     gene            complement(5304..5705)
                     /locus_tag="PKN80_05705"
     CDS             complement(5304..5705)
                     /locus_tag="PKN80_05705"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNQ35543.1"
                     /translation="MVEKGVVVEKSGRRVSIRMTAPGDCDRCGICAGGRRRLLELERS
                     DLEVGDRVRVEIPDSALVRFSILFYLLPALVFALGFGLGAALWPGWWGGLAGGAAGLA
                     AVFAWGRFSRSGGRPEIEVHKEEGDEDARED"
BASE COUNT         1038 a         1916 c         1935 g         1010 t
ORIGIN      
        1 ggccccgggc gtcgaaccag gccgacacgc cggtgttggc ggcgcggagc agcggggtcc
       61 ggttctcggc cgcccggagc gcggcggcgg ccaggtgctg gtaagcgccc aggcgtccca
      121 gccaggagtc gttggtcagg gtgatcatga gaccgggatg acggcgctgg gtatcggccg
      181 ccgcctcggc gaagatgtcc tcgaagcaga tgtaaagcgt caggggaacc aaaccgctcc
      241 ctttgggcag ccggatgccg gccggaaggt caccggcgct caaaccgggc aggaaaccag
      301 cctggcgttc gaccagccag cggaccgggg gcaggttgcg gcccggggtg tattcgccga
      361 acgggaccag gtggcgtttg cggtaaaccg ccagcagccg gccgcccggg gcgtagaggg
      421 cggcgctgtt gtaagcccgg ccggcggaat cgacctccag cacgccgcat aaaaggtggg
      481 cgcgcttttc gcgcagcagg cgccggacgg ccagcggcgg ccgctcaatg gaaccgctga
      541 ccagggttgt ctccggccag atgatcaggt cgaccgggct ggaaagctgg cgggtgacat
      601 agaggtaggg ttccaacggc ggatcctgcc ggcgcaggct ggagggaacg ttgccctgga
      661 cgacggcgaa gcggaggatc ggtgcgtccg gaggcggaac cggcggccgg aaggagaggg
      721 cctggccggc caggaaggcg gccaggacca ccagcaggcc aggccagccg gccgaacgac
      781 ggccggacgg acggctgaag aaggagaaga ggctgaagtt ggccagaacg accaggaagg
      841 cgaccagcgg tccgccgccg aaggaaagta gcggctggat gagcggattc cagtgctggg
      901 taatcgccag gttgagccag ggcaggccgg tgaagaggtg gccgagaatg aactccagga
      961 ccacccagag ggcggcggcc cagaggtcgc gcaggcgacc gcgcgcccgg ctggccaggt
     1021 aaaagaagag accgggatag atggccaggt agagggtggc ggcaaaccaa cccaacccgg
     1081 agaggcgcac cagccagagg aggccgaggc cgaagacggt catcccccag acgtagcccg
     1141 accagaaaga ccggccgcgc cggagcagga agaggatggg cagcaggccg aaccaggcca
     1201 ggtagccctg gttgaagggc gggtaggcca ggaggaagaa taccccggcg ataaaggccg
     1261 ccaccagtat gctgaggcgc ttcaagccgg ggctcccgat gaggggggct gatgatcgta
     1321 ctttatccgc aggttccggc cacggccgta ttcccggtcc cagatcggat cgaagatccc
     1381 gtgaatggac cgggagtaat tgtcgaggta tccgtttatc tcgtgcagaa ggcaccggcc
     1441 gccgtggtcg gtgtcgtgag cgcccgtctc ccggacgacc tcgcgcagga cccagggctg
     1501 gtcgatgatc atgcagggcg tcatgaggtt gggcgagtaa ggctgccgct tctggatggc
     1561 ccgcatcagg gggctgttga tggcctcgcg caggctgcgc tcccggatgt tgtcgatcgc
     1621 gaaatggacg aagacgcagg gttcgacgtc gccgttggca ttgatgtgca ggtaggtgcg
     1681 gccgccggcg atgcagccgc cggtaaggtg gccgtcattc cagaaatcgg ccaggatgat
     1741 cggcttgcgg ttgcgcaggt cgttcacccg ttcccgggca taggcgcgct gttcgggagt
     1801 cggcatcagg ttgacatcgg gacgcgaacc gatcggaatg tagtgaaaat accagccgaa
     1861 gagggcgccg gcctcgatca tcgaatcaat gaacccgtcg ctgaagagca gttccaggtt
     1921 gtggcgggtc ggcgtggcgg aaaagccgaa cataaccccc ttctccacca gctttttcca
     1981 cacctgctcc agtttccggt agacgccggt gccgcgccgg tggtcggtct cggcctggta
     2041 accctcgacg ctgatggccg gcgccacgtt gcccagggcg gccagccggt cggccacccg
     2101 gtcggtgatc agggtaccgt tggtgtaaac ctggaagtag atgtcgttgt ggcgctgcat
     2161 gagatcgagc aggtcttcgc gcatgaaagg ttcgccgccg gagatggtaa caaagtaaat
     2221 gcccagttcc ctggcctggt tcaaaatacg gtcaagatcg gcggaggcca gcacgtcctg
     2281 gcgttcgtaa ctgccggcgt aacagccgat acaattcagg ttgcagcgca tggtggggct
     2341 gatcacgatg aagaagggcg gccgccagcc ctctgactcc cggatggagt tgcggcggcg
     2401 ggtacccagc agcataccgt tcacgaccag gttgtggatc aacttctcgc gggccgggac
     2461 cgaaaggcgg cgggcaatcc gcttggcggc ctccaggctg gggtgattcc gttcgaactt
     2521 ctccctgatg acgcgcaccc gttccttctg ggcggtatcg gccaccagga gttcggccag
     2581 gtgggtaaga cggatcaggg tggtgttgga aattcgtggc atcaaagcca ggatggcttt
     2641 taccgtccgg gtcgcggcat aaccggtggc tttttcacgg gcttgctgac ctaaacgcat
     2701 ggacgcctcc ttcactatct gatttctttt atgcggttct cctggtcggt gagcagaatc
     2761 ctgggttgga agccgcggag ttcttccgcg ctataaaaag cggtcgaaag gacgattacc
     2821 cggtcgcccg cctcgaacca gcgggcggcg gcgccgttca ggcagacctg gccgcggccg
     2881 ggctcgcccg ggatatagta ggtctcgatc cgggcgccgt tggcaaggtt caggacctgg
     2941 accatctcga acggcagcag atcggccttt tcggccaggg cggagtcaat ggtgatgcta
     3001 ccgggatagt tgaggtccgt cccggtcacc accaatccct ggatcttgga cttgcagaat
     3061 tgtctgagca tcttcttctc tcggtacttt aaaaaacgcc cctttttccc cgtcccaacc
     3121 agcccggaga ggtgccagca gcaacgcact ctttgcaact gcaattattt tagcccgtcc
     3181 ggcgactccg gtcaagtaat gacggcggtc ggccggtttg aaaattcggc cggccggtga
     3241 taagatttcc ggataagacc ggtccgaatc caatccagaa tcgggaggat aaatgaatct
     3301 ctggttggtc atcctgctta ccgccctgct cttccacctg gcgcaccgcc tttacagccg
     3361 ggggctggcc cgggtgctgg gcgaggagcc ggaacggccc accccggccg tcacgcgcaa
     3421 cgacggctat gattacgtcc cgtccaagac actggtggtc ttcgcccacc atttcgcctc
     3481 gatcgccggc gccgggccga tcatcgggcc gaccctggcc ctcctctacg gcttcgtacc
     3541 cgtctacctg tggataatca tcggcggcat cttcatcgga gccgcccacg atttcagcgc
     3601 cgtcttcatc agcctccgga accagggccg ctcgatcgcc gagatcgccc gcggccagct
     3661 gggaaacacc ggttacttcc tcttcgtgct cttcgccatc atggtgctca ccctggtcaa
     3721 cgccgccttt ctcaacctct cggtgaccgc gctgacctcg caggcgccgc tggcggcctt
     3781 cggcctcggg ccgggacagc acctgctgcg cgtcatccag gactccggcg gccagatata
     3841 cggcttcatc ggcggcatcg cctccacctc ggtgatcttc atcaccctgc tggcgcccct
     3901 ggtcggctgg ctgctctacc ggcggggcct caacccggtg gccgggagtt tcctggccct
     3961 ggccatctgc ctggccgcgg ttgccttcgg cctggcctac ccggtcagcg tgccgccggc
     4021 ctggtggcgg gtgatcctga ccatctatgt cttcgcggcc gccggcatcc cggtttggct
     4081 gctgctgcag ccgcgggact tcaccaacgt ccagatcctt tacctgggca tcggcctgat
     4141 gctgatgggc atcctggccg ccggcttcca gggcgcgccg atggccgtgc cggccttcaa
     4201 tgtccaggcc gcccaggcga aacccgccct gggccagctc tggcccttcc tcttcatcac
     4261 ggtcgcctgc ggggccatct cgggcttcca cgccctggtg gccggcggca ccaccgccaa
     4321 gcaactgtcg cgggaagggg ccgcccggac ggtcggcttc gacgccatgc tcctggagtc
     4381 gatcatggcg gtcgcggtca tcatcaccct gagctgcggg ctcgacttca gccactacca
     4441 ggcggtggtc tttccggcgc cggagagcgg actgcaatcc aacccgatcc tggccttcgc
     4501 cctggccctg ggaggcttgc tcaaccgcgg tttcggcatg ccgacagccg tcggcaccat
     4561 cttcgggata ctgatgctgg aggggtttat catcacgacg ctggatacca gcatccggct
     4621 ccaacgttac ctcttcgagg aggtctgggg gataatcttc cagaagccgg ccggctggct
     4681 gaaatcatat tatttcaatg ccggcctggc ggcgctggtc atgttcctgc tggcccgggg
     4741 caatgcctac acgatcatct ggccggtctt cggagccgcc aaccagctgc tggccgccct
     4801 gaccctgatc accgtttcgg tctggctgac ccggcgggga cggggacgcg ccctcttcct
     4861 ggtcccggcc attttcatgc tggtcacgac catcgccgcc ctctggctga ccctggtccg
     4921 gaagttcctg cccggccgca actacccgct gatcgccgcc gccctcttcc tgatgctgct
     4981 ggccgtctgc gtggccggcc tggccgccgg ccggctgatc aggaaggagc cggccgattc
     5041 gcggacctga cctgaaaaag agaagggatc aggcggccgg accggacggc tcgtcggtat
     5101 cgaccgactc taccgacttg acttccggca cccgctcctt gaccagcttt tcgacccgca
     5161 tcttgagcgt caaagcggca aacgggcagt ggccgcaggc gcccatcagc cgaaccttga
     5221 cccggccgtc atcgctgatc tcgaccagtt cgacgtcacc gccctccatc cgcaggaact
     5281 cccgtacctc gtccagggcc ttctcaatct tctcgcgcat cttcgtctcc ttcttccttg
     5341 tgaacttcga tttccggccg gccgccggac cgggaaaaac gcccccaggc aaagaccgcg
     5401 gccaggccgg ccgcgccgcc ggccaggccg ccccaccagc ccggccagag cgcggcgccc
     5461 aggccgaacc cgagggcaaa aaccagggcc ggcaacaggt aaaaaagaat tgaaaaacga
     5521 accagggccg agtcgggaat ctccacccgg acccggtcac ccacctcgag atccgatctt
     5581 tccagttcca gcaaacgccg gcggccgccg gcgcaaatcc cgcagcggtc acagtcgccg
     5641 ggagccgtca tccggatgga gacccggcgg ccggattttt caacaaccac gcccttttcc
     5701 accatggcat caaggccggg ttcagatgaa cagcccggcc acctcggaac ggtccagcgc
     5761 cgaagcggtg accgtttcga ccggatcgga gatccggacc ggcaccggtc cggccgccga
     5821 ctcgtcagtg taggccgcca cgatggcggc cgccttcaaa agatccgccc ggccgggccg
     5881 gccgagcacg accgccgcc
//
LOCUS       DAOQYN010000118         5877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_13864_length_5877_cov_5.495534, whole genome shotgun sequence.
ACCESSION   DAOQYN010000118 DAOQYN010000000
VERSION     DAOQYN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13864_length_5877_cov_5.495534"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKN80_05710"
     CDS             <1..279
                     /locus_tag="PKN80_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35544.1"
                     /translation="EIEFLGGYLDYIVWHLDGPEMIKHLDILLDLPQIKAIQWVHGSG
                     HPSVSHWLPLLKRIQAKGKSLVLSCTYGAEEKDILVRELDPVGLYISY"
     gene            302..2104
                     /gene="mnmG"
                     /locus_tag="PKN80_05715"
     CDS             302..2104
                     /gene="mnmG"
                     /locus_tag="PKN80_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNQ35545.1"
                     /translation="MTPDFDVIVVGAGHAGAEAALAAAGSGLTVLVLTLNLETVGQMS
                     CNPAIGGVAKGQLVREIDALGGWMGLWADQAGIQFRLLNSAKGPAARSPRAQCDRKIY
                     RSLVRKTLEDNERITLRPEMVTEVLTAGGTVRGVKTRSGRTWMSRAVVVTAGTFLAAV
                     LHQGESIQPGGRMGEEAAEELSRSLAGLGLEKRRFKTGTPPRVSGNSINLGATTIQPG
                     DIDPKPFSYRTRDFQPLQVPCYLTRTNAETIRVVRENLHQAPLYTGQILGTGPRYCPS
                     LEVKVRQFPDKTRHPIFLEPEGRDTDEVYVNGFATSLPVDVQEKALRTVAGLEEVRVL
                     CHGYAVEYDYFPPWQLKPTLETRRVAGLYLAGQVNGTSGYEEAAGQGLLAGINLALKL
                     KERPPLLLSRSQAYLGVMISDLTTMDLQEPYRLFTSRAEYRLLLRADNADFRLMETGW
                     QLGLIDEPARRRMLEQREAVATELRRLRNRYLPGLKEAVSLEQYLRRPGKTYRDLDEL
                     GQGRSLGARIRETVEIEAKYAGYLDQDRTLAERMAAEMEMKIPEKFNYASITGLSAEG
                     REKLEKFRPATLEAARRLDGLRASDLALIFLRLR"
     gene            2101..2583
                     /gene="tsaE"
                     /locus_tag="PKN80_05720"
     CDS             2101..2583
                     /gene="tsaE"
                     /locus_tag="PKN80_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNQ35546.1"
                     /translation="MKIKKTTGSAGETRALAAELARGLKGGEVISLSGPLGAGKTVFV
                     QGLARGLGISEKEVRSPSFVILNEYPGRLPLFHFDFYRLRSAAELATVPFRERLAQKG
                     VVVMEWPERAAGLLPAGLIRIEIRVLDRTRREIGIERPARTQKRRLSTAARKSGALKR
                     "
     gene            complement(2529..3173)
                     /gene="rpe"
                     /locus_tag="PKN80_05725"
     CDS             complement(2529..3173)
                     /gene="rpe"
                     /locus_tag="PKN80_05725"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNQ35547.1"
                     /translation="MVKISASILNSDFSRLAEEIRRAESAGVDFLHLDIMDGHFVPNL
                     TVGPQVVESLRPHTRLPFEVHLMITDPAQYAPAFIKAGSDLILFHLEVVARPEKLLAE
                     IQASGARAGLVVNPETPAEPVLPFIHLVDQVLVMTVRPGFGGQKFIEASLEKVAVLDG
                     ERRRRGLKFAIEVDGGVNPETGRRCAAAGVDILVAGTYLFKAPDFRAAVESLRF"
     gene            3251..3706
                     /gene="smpB"
                     /locus_tag="PKN80_05730"
     CDS             3251..3706
                     /gene="smpB"
                     /locus_tag="PKN80_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNQ35548.1"
                     /translation="MARSEIKKVSILNRRAHHDYEIMETVEAGLVLSGPEVKSVRTGS
                     ASLAEAFGRVDRGEVHLYNMYIAPYAPSREEGDPRRPRKLLLHRAEIRKLENGLQHGL
                     ALIPLRLYFRKNWAKVELALGRGRRKYDKRERIKTREAEREIKRSISRR"
     gene            3722..4064
                     /gene="ssrA"
                     /locus_tag="PKN80_05735"
     tmRNA           3722..4064
                     /gene="ssrA"
                     /locus_tag="PKN80_05735"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4148..5137)
                     /locus_tag="PKN80_05740"
     CDS             complement(4148..5137)
                     /locus_tag="PKN80_05740"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNQ35549.1"
                     /translation="MDPLKFINHYRSFIITGHLSPDGDAIGSEIAFYFFLRDLGKETI
                     IVNDEPAPESFSFLPGFEHIYTLDEFHDHYGPRHDFEAAVFMECSSRQRAGRTERLTR
                     GLPALNIDHHFDNRNYGTVNLVVPQAAACGEVIYELIEGAGARISPEMAAALYTAILT
                     DTASFRFQTTPRTLRSAADLIELGADPEKVAAGAYEQVPFKTFKLLARSLETLKQACN
                     GLVTYCRVSRRMYEETGTTDSNSESFIDYLRVIARSRVNIVFKELPDHRTRVNLRSKG
                     DLDVQRVASHFGGGGHRNAAGCSLDAGFEEARNLVLAEVKKQLPHHGEKERSA"
     gene            complement(5125..>5877)
                     /gene="hflX"
                     /locus_tag="PKN80_05745"
     CDS             complement(5125..>5877)
                     /gene="hflX"
                     /locus_tag="PKN80_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNQ35550.1"
                     /translation="HANSAEGKLQVDLARENYRLSRLTGRGVALSRLGGGIGTRGPGE
                     MKLEEERRHIRESIRRLEERIVKLKKQRQRTRELRRSRGLPEVALVGYTNAGKSSILN
                     ILCGRPAARVADQLFATVDPTSRRLHLGQNRFAVITDTVGFVHNLPHTLVAAFHATLE
                     GLGEAHLLLLVLDGGSPLLFEHYRTTCQVMSEVRADRQPVLLIVNKIDQLDTPDRERL
                     KRKLPEARLVSARTGEGISELREDIYKRLWTP"
BASE COUNT         1121 a         1878 c         1872 g         1006 t
ORIGIN      
        1 gaaatcgaat tcctgggcgg ttacctcgat tacatcgtct ggcacctgga cggtccggag
       61 atgatcaagc accttgatat cctgctcgac ctcccccaga tcaaggccat ccagtgggtc
      121 catggcagcg gccacccgag cgtctcgcac tggctgcccc tgctgaagcg aatccaggcg
      181 aagggcaaaa gcctggtact cagctgcact tacggcgccg aggagaagga catcctggtc
      241 cgggaactgg acccggtcgg cctctacata agttactgag cccgccgcgg cctgaataaa
      301 catgaccccg gatttcgatg tcatcgtggt gggggccggc cacgccgggg ccgaggcggc
      361 cctggccgcc gccggctccg gtctgacggt cctggtactc accctgaacc tggaaacggt
      421 gggacagatg tcctgcaacc cggccatcgg gggagtggcc aaggggcaac tggtccgtga
      481 aatcgatgcg ctgggcggct ggatgggcct ctgggccgac caggccggca tccagtttcg
      541 cctcctcaac tccgccaagg gaccggcggc ccgcagtccc cgcgcccagt gcgaccgtaa
      601 aatctaccgg tctctggtac gcaaaaccct ggaagacaac gaacggatca cgctccgacc
      661 ggagatggtc accgaggtcc tgacagccgg cggcacggtc cggggagtca agacccggag
      721 cggacggacc tggatgagcc gggcggtggt ggtcaccgcc ggcacctttc tcgcggccgt
      781 gctgcaccag ggggaatcaa ttcagccggg cggccggatg ggtgaggagg cggccgaaga
      841 gttgagccgg tccctggccg ggctggggct ggaaaaaagg cgtttcaaga ccggcacccc
      901 gccccgggtc tccggcaatt cgatcaacct gggcgcaacc acgatccaac cgggtgatat
      961 cgatccaaaa cccttttcct accggaccag ggactttcaa cccctacagg ttccctgtta
     1021 cctgacccga accaacgccg aaaccatccg ggtggtccgg gaaaacctgc accaggcgcc
     1081 gctttatacc gggcagatac tgggcaccgg gccgcgttac tgtccttccc tggaagtaaa
     1141 ggtccgccag ttcccggata agacccggca ccccatcttc ctggaaccgg aggggcgcga
     1201 taccgacgag gtctatgtca acgggttcgc caccagcctc ccggtggacg tgcaggaaaa
     1261 ggccctgcgc acggtggccg gcctggaaga agtccgggtc ctgtgccacg ggtacgcggt
     1321 cgagtatgat tattttccgc cctggcagct gaaaccgacc ctggagaccc gccgggtggc
     1381 cggcctctac ctggccggcc aggtgaacgg aaccagcggt tacgaagagg cggccgggca
     1441 gggcctcctg gccgggatca atctcgccct gaaactgaaa gaaaggccgc ccctgctcct
     1501 ctcccgcagc caggcctacc tgggcgtgat gatcagcgac ctgaccacca tggatctcca
     1561 ggaaccttac cggctcttca cctcccgggc tgaataccgg ctcctgctcc gggccgacaa
     1621 tgccgacttc cggctcatgg agaccggctg gcagctcggc ctgatcgacg aaccggcccg
     1681 gcgccggatg ctcgaacagc gggaagcggt ggcaaccgaa ctccggcggc tccgtaaccg
     1741 ctacctgccc ggccttaaag aagcggtctc cctggagcag tatctccgcc ggcccggcaa
     1801 aacctaccgg gacctggacg agctcggcca ggggcgctcc ctcggggcca ggatccggga
     1861 aaccgtcgaa atcgaggcca agtacgccgg ttatctcgac caggaccgga ccctggccga
     1921 gcggatggcc gccgaaatgg aaatgaagat accggagaag ttcaattacg cttcgataac
     1981 cggtctttcg gccgaagggc gtgaaaagct ggagaagttc cggccggcga ccctggaagc
     2041 ggcccgccgc ctcgatgggc tgcgggccag cgacctggcc cttatcttcc tgaggctccg
     2101 ttgaagataa aaaaaacgac cggttccgcg ggggaaaccc gggcgctggc ggccgaactg
     2161 gcccgggggt tgaaaggcgg tgaggtcatc tcgctttccg gcccgctggg cgccggcaag
     2221 accgtttttg tccaggggct ggcccggggc ctgggtattt ctgaaaagga ggtgcgcagc
     2281 ccatccttcg tcatcctgaa cgagtatccc ggccggctgc ccctcttcca cttcgacttc
     2341 taccggctcc ggagcgctgc cgaattagcc acggtgccct tccgggaacg gttggcccaa
     2401 aaaggcgtgg tggtaatgga gtggccggag cgggccgccg gtctcctgcc ggccggcctg
     2461 atcaggatcg aaatccgggt cctggaccgc acccggcgcg aaatcggcat cgaaaggccg
     2521 gcccggactc agaaacgcag gctttccacc gccgcccgga agtcgggcgc cttgaaaaga
     2581 taggtgccgg ccaccaggat atcaaccccg gccgccgcgc agcgccgccc ggtctcgggg
     2641 ttgactccgc catccacctc tatcgcgaat ttcagacccc gccggcggcg ttcaccgtcc
     2701 aggaccgcca ccttttccag cgaggcctcg atgaacttct gtccgccgaa gccgggccgg
     2761 accgtcatga ccaggacctg gtccaccagg tggatgaagg gcagcaccgg ctcggccggg
     2821 gtctccgggt tgaccaccag cccggccctg gccccggaag cctggatctc ggccagcagc
     2881 ttctccggcc gggccaccac ctccaggtga aagagaatca agtcgctccc ggccttgatg
     2941 aaggccgggg cgtattgggc cggatcggtt atcatcaggt gcacttcgaa gggaagccgg
     3001 gtatgcggcc tgagactctc caccacctgg gggccgacgg tcaggttggg cacgaagtgg
     3061 ccgtccatga tgtccaggtg caggaaatcg acgccggccg actcggcccg ccggatctct
     3121 tcggccagcc gggagaaatc ggagttgaga atggaagccg aaattttaac cattttgctc
     3181 ctcacaacgg ccgcccgccc gggtccggcc gacacggaat tgactgattt gcgctataat
     3241 atattatcat atggcccgat cagagattaa gaaggtctcc atcctcaacc ggcgcgcgca
     3301 tcatgactat gaaatcatgg aaacggtcga ggccggcctg gtcctgtctg gaccggaggt
     3361 caaatcggtg cggaccggct ccgcttcgtt ggccgaggcc ttcggccgcg ttgacagggg
     3421 ggaggtccat ctctataata tgtatatcgc cccttacgct cccagccggg aggagggcga
     3481 tccgcggcgg ccgagaaaac tgctgctgca ccgggcggag atccggaaac tggaaaacgg
     3541 ccttcagcac ggcctggcgt tgattccgct gcgcctctat ttccgtaaga actgggccaa
     3601 ggtggagctg gccctgggcc ggggccgccg caagtatgac aagcgggagc ggatcaagac
     3661 ccgcgaggcg gaacgggaga ttaaacgcag tatctcccgc cgctgactaa atattcagaa
     3721 agggggcgaa tcgattcgac tggaagtcaa gctgcggcct ggagcatgcc gagtttgggg
     3781 aagggctcgt caaaatcccc aaagaaacaa ctgccgaaac ggcaaactac ctggcggcct
     3841 aaccgtcggg ctcccctctg aaggcctgcg ttcaggggat ggagaggcag cgggctggtt
     3901 ccttcaatct gtctcgggtt gaaggaacga gaaaaatccg gggctaagga acggctatcc
     3961 ggtgccgggg agcgcctgac ccgaaaaaca aaactggcaa caagcatgta gtgccgaccg
     4021 cacttatttc caggacgggg gctcgctacc ccccgcctcc accgcttccc ttccggggag
     4081 caaaaaaagc gccggggaca atcttttccc cggcgctttt ttgtttgagc ccgtcaaaaa
     4141 aaagcggtca ggccgaccgc tccttttcgc cgtggtgcgg cagctgcttc ttgacctcgg
     4201 ccaggaccag gttccgggcc tcctcgaaac cggcgtccag gctgcagccg gccgcattgc
     4261 ggtgtccgcc gccgccgaag tgggaggcaa cccgctgcac atccaggtcc cccttggaac
     4321 gcaggttgac ccgggtccgg tggtccggca gctccttgaa aactatgttc acccggctcc
     4381 gggcaatcac ccggaggtaa tctatgaaac tctcgctgtt gctgtcggtg gtcccggtct
     4441 cctcgtacat gcggcggctg acccggcaat aggtgaccag gccgttgcag gcctgcttga
     4501 gggtctccag gctgcgggcc agcagcttga aggtcttgaa gggcacctgt tcgtaagccc
     4561 cggccgcgac cttctccgga tcggccccca gttcgatcag gtcggccgcg ctgcggaggg
     4621 tgcgcggcgt ggtctggaac cggaaagagg ccgtatcggt caggatggcg gtatacaggg
     4681 cggcggccat ctccggcgaa atccgggcgc ccgcgccctc gatcagctcg tagatgacct
     4741 ctccgcaggc ggccgcctgg ggcacgacca ggttgaccgt tccgtagttg cggttgtcaa
     4801 agtggtggtc gatattgagg gcgggcagcc cccgggtcag gcgctcggtc cggccggccc
     4861 gctggcggct gctgcattcc atgaacacgg ccgcttcaaa atcatggcgg ggaccgtagt
     4921 ggtcgtgaaa ttcgtccagg gtgtaaatat gctcgaaacc gggcaggaag gagaaggact
     4981 ccggggccgg ctcgtcattg acgataatcg tctccttccc caggtcacgg agaaagaaat
     5041 aaaaggcgat ctcgctgccg atggcgtccc catcgggcga aagatggccg gtgatgatga
     5101 aggaacggta atggttgata aatttcaggg ggtccataat cttttgtaga tgtcttccct
     5161 caactcggat atgccttccc cggtccgggc cgaaaccagc cgggcctccg gcagtttccg
     5221 tttcaaccgc tcccggtccg gagtatccag ctggtcgatc ttgttcacga tcagcaggac
     5281 cggctggcgg tcggcccgga cttcgctcat gacctggcag gtagtccggt aatgttcaaa
     5341 taaaaggggt gaaccgccgt ccagaaccag caggagcagg tgcgcctcgc ccaggccttc
     5401 cagcgtggcg tgaaaagccg ccaccagcgt gtggggaagg ttgtgaacga agcccaccgt
     5461 gtcggtaata accgcgaacc ggttctggcc caggtgcagc cggcggctgg tcgggtcaac
     5521 ggtggcgaaa agctggtcgg ccacccgggc ggccggacgc ccgcagagaa tgttcaggat
     5581 cgaactttta ccggcattgg tgtatcccac cagcgccacc tccggcaacc cccggctgcg
     5641 ccggagctcc cgggtccgct gccgctgctt cttcagcttt acaatccgtt cttccaggcg
     5701 gcggatgctc tcccggatat gccggcgctc ctcctccagc ttcatctcac ccgggccccg
     5761 ggtaccgatg ccgccgccca gccgggagag agccaccccc cggccggtca aacgtgaaag
     5821 ccggtaattc tcacgcgcca ggtcgacctg gagttttccc tcagcgctgt tggcgtg
//
LOCUS       DAOQYN010000119         5874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_13876_length_5874_cov_4.013920, whole genome shotgun sequence.
ACCESSION   DAOQYN010000119 DAOQYN010000000
VERSION     DAOQYN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13876_length_5874_cov_4.013920"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PKN80_05750"
     CDS             complement(<1..779)
                     /locus_tag="PKN80_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35551.1"
                     /translation="MSKSRPPAEGAANTGKLRKEAEKLLAEWPERLALVPEIKPLSAA
                     WLPRTSPFSGRRGPARRYGAWWEWNRKEPDRLRDLTTGRPLPDAFPYRNVAAMYRYQA
                     SIGRMWSAQTLAEAYRATGDARFARAVLDLIRRLGEVMPGYPVTRETDANPERLITYG
                     PEARGTFYNIKVSVGWVDTLTLQILMRAYRLVRDCPQLKAADHRAVAGLAREIIEFQS
                     LPTFLYLHDKFHNSLTNHLEAFILAAQLLGPDFQVRDTQSGS"
     gene            complement(877..1617)
                     /locus_tag="PKN80_05755"
     CDS             complement(877..1617)
                     /locus_tag="PKN80_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNQ35552.1"
                     /translation="MEKIVRVTVWNEFRHEKKSEAVRAIYPEGMHAVIAGHLNRQSGL
                     RAAAATLDEPEHGLTEAVLAETDVLTWWGHTAHHLVADEIVERVHRRVLDGMGLVCLH
                     SAHLSKIFRRVLGTSGALRWRDIGEKQRFWNLEPGHPVTEGLGEYFELPAEEMYGERF
                     DIPTPDKLVFISWYAGGDVFRSGCCWERGHGRIFYFQPGHELYPSFHDPNVLRVITNA
                     VRWAAPRIIQTEKCRQAKEPLEEVPPKA"
     gene            complement(1672..2736)
                     /gene="mraY"
                     /locus_tag="PKN80_05760"
     CDS             complement(1672..2736)
                     /gene="mraY"
                     /locus_tag="PKN80_05760"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNQ35553.1"
                     /translation="MLYQLLYLLKDFWFGFNVFRYLTVRMVLGAFTSLFAVLYFGPGF
                     IRSLRHLKHPDLLEFHASKYGTPTMGGILIIGSILLSVLLWADLSNKYLLLSLLVMLW
                     LGATGFYDDYTKFIGNQAKGVRPLVKIVSQVALGLTVGLILFFGPFEVATTIYPPFFK
                     EIIWELGAWYLALVILVITAASNAVNITDGLDGLAIGSVLMVAITFTAISYIVGNYVF
                     ANYLGITFIRGTEELAVLCASLVGASLGFLWFNAYPAEIFMGDTGSLALGGVLGIVAI
                     LIKQEFALLFAGGIFVLEALSVIIQVVSFKTRGKRVFKMTPIHHHFELLGWPESKLVI
                     RFWILAIIFALFSLVTLKIR"
     gene            complement(3075..4877)
                     /locus_tag="PKN80_05765"
     CDS             complement(3075..4877)
                     /locus_tag="PKN80_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35554.1"
                     /translation="MERKRRWGFTYVEIAMVTSLVTSVSMTAYQAVKKGKEANCLNNL
                     RQVYIALNICAMDYGALPEARFFPSSASDPRGIHNVLKKYVSNPAIFYCPSIPEELNK
                     SGTNYLWNDLVNRNDLSSLPQGTWIMTEATAVRSDIPPPHTGGYGTLAPDGSATIRRR
                     PPIPEVRSTPAAPAAPETPAAPGIPAAPATPPAPGTPAGPETPSAPAAPGTPAAPAAP
                     GAGLRFRFDAFGPSLEAGAQQVKISVVDESGAPYPLDAPVWVLDLAESLRPETILIQK
                     GTWQGPINFTRPAAENRLVLLGEKNSGASPVFSVTAGPAEKVRLTPAGPARAGEPVKL
                     EIQVTDRFGNPAAFTGKASLASDDPEAKLPAELSLSGPAGPELTFFKAGKRKLRVKIS
                     GAAAGELEGETEVAVRPGPLDRFRIDPIRSPQVAGQPFTVFIRSEDRWGNRVPGLELN
                     DPSHTLKPVRESIIAFSRVEEVTITRAGREAAVEVADGLGHTGRSNAFEVLPARPEII
                     RLEGLPAVLFRGREYRVSASLGDAFGNRIEAAPEKLEITSVTPGFKSVPVPGKTEFLL
                     TFDTVGRQVLTVNERDGQLKLELELFVLEKPESP"
     gene            complement(4904..5323)
                     /locus_tag="PKN80_05770"
     CDS             complement(4904..5323)
                     /locus_tag="PKN80_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015419253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ35555.1"
                     /translation="MPIKPAEARELRFVVHEHFARRHHFDFRLELDGVLKSWAVPKAP
                     PEAAGEKRLAVEVPDHPLDYGDFEGLIPEGEYGAGRVEIWDRGPYRLIERRPEKIVFE
                     LRGEKLRGVYILLFFKADRQRNWLFFRPAEGAEKPGR"
     gene            complement(5290..>5874)
                     /gene="nth"
                     /locus_tag="PKN80_05775"
     CDS             complement(5290..>5874)
                     /gene="nth"
                     /locus_tag="PKN80_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNQ35556.1"
                     /translation="TRTALKFQTPFQLLVATILSAQSTDEQVNRATPNLFARMAGPAA
                     FNRASQAEIEKLIGSINFYRNKAGYLKRMSGILINRHGGKVPRNMEELVRLPGVARKT
                     ANVVLSQVFRKNEGVVVDTHVARVSQRLGLTRETDPVKIETDLMAILPQRDWGNFAFR
                     LILHGRWVCQARKPACAGCCLEDLCPSSLLKQES"
BASE COUNT          930 a         1807 c         2113 g         1024 t
ORIGIN      
        1 gagccggact gggtgtcgcg gacctggaaa tccggcccca gcagctgggc ggccaggatg
       61 aaggcctcca ggtggttggt cagcgagttg tggaacttgt catgcaggta gaggaaggtg
      121 ggcaggctct ggaattcgat gatctcccgc gccaggccgg cgaccgcccg gtgatcggcg
      181 gccttcagct gcgggcagtc ccggaccagc cggtaggccc gcatcaggat ctggagggtg
      241 agggtgtcca cccagccgac gctcaccttg atgttgtaga aagttccccg cgcctccggt
      301 ccgtaggtga tcaggcgttc cgggttggcg tcggtctccc gggtgaccgg gtaaccgggc
      361 atgacctcgc cgaggcgccg gatgaggtcg agaacggccc gggcgaatct ggcgtccccg
      421 gtcgcccggt aggcctcggc cagggtctgg gcggaccaca tccggccgat cgaggcctgg
      481 tagcggtaca tggccgccac gttgcggtag gggaaggcgt ccgggagcgg ccggccggtg
      541 gtcaggtcgc gcaggcggtc gggctccttc cggttccact cccaccaggc gccgtaccgg
      601 cgggccggtc cgcgccggcc gctgaagggg gaggtcctgg gcagccaggc ggccgaaagc
      661 ggcttgattt caggcaccag ggccagtcgc tccggccatt cggccagcag tttctcggcc
      721 tccttccgaa gtttgcctgt gttcgccgcg ccttcggccg gcggccggga cttgctcatg
      781 cttcttcctc cctgtctgga ttgccgttgg aagcgaccgc gcccggaagc ggggcggccg
      841 tcggtcccgg acgcgaaggt ccgggaccgg gtccggtcag gccttgggcg ggacctcttc
      901 caggggctcc ttggcctggc ggcacttttc ggtctggatg atccggggcg ccgcccagcg
      961 caccgcgttg gtgatgaccc tcaggacatt cggatcgtgg aagctcgggt agagttcgtg
     1021 gccgggctgg aagtagaaga tccgtccgtg gccccgttcc cagcagcagc cgcttcggaa
     1081 gacgtccccg ccggcgtacc aggagatgaa gaccagcttg tcgggggtgg ggatgtcgaa
     1141 gcgttcgccg tacatctcct cggcgggcag ctcgaagtac tcgcccaggc cttcggtgac
     1201 cgggtggccg ggctccaggt tccagaagcg ctgcttctcg ccgatgtcgc gccagcgcag
     1261 ggcgcccgag gtccccagga cgcgccggaa gatcttcgag aggtgggccg agtgcaggca
     1321 gaccaggccc atgccgtcca gcacccgccg gtgcacccgc tccacgatct cgtcggcgac
     1381 caggtggtgg gcggtgtggc cccaccaggt cagcacgtcg gtctcggcca ggaccgcctc
     1441 ggtcaggccg tgctcgggct cgtccagggt ggcggcggcg gcccggaggc cggactgccg
     1501 gttcaggtgg ccggcgatga ccgcgtgcat gccctccggg taaatggccc gcaccgcctc
     1561 gctcttcttc tcgtggcgga attcgttcca gaccgtgacc ctgacgatct tttccattgg
     1621 tttctccttg tttgtctttt tccggaaggc gttttttccc gtcccggcgg ctcagcgtat
     1681 cttgagcgtc accagcgaga agagggcgaa gatgatggcc agtatccaga agcggatgac
     1741 cagtttcgat tccggccagc cgagcagttc aaagtggtgg tggatggggg tcatcttgaa
     1801 gacccgcttg ccccgggtct tgaaggagac cacctggatg atgaccgaga gcgcctccag
     1861 gacgaagatg ccgccggcga agagcagcgc gaattcctgc ttgatgagga tggccacgat
     1921 gcccagcacg ccgcccaggg ccaggctgcc ggtgtcgccc atgaagatct cggccgggta
     1981 ggcgttgaac cagaggaagc ccaggctggc gccgaccagg ctggcgcaga ggacggccag
     2041 ctcctcggta ccgcggatga aggtgatgcc caggtagttg gcgaagacat agttgccgac
     2101 gatgtagctg atggcggtga aggtgatggc gaccatcagc acgctgccga tggccaggcc
     2161 gtcgaggccg tcggtgatgt tgaccgcgtt ggaggcggcg gtgatgacca ggatgaccag
     2221 cgccaggtac caggccccca gctcccagat gatctccttg aagaagggcg ggtaaatggt
     2281 ggtggccacc tcgaaggggc cgaagaagag gatgagcccg accgtgagtc ccagggccac
     2341 ctggctgacg atcttgacca gcggccggac gcccttggcc tggttgccga tgaacttggt
     2401 gtaatcgtcg tagaagccgg tggcgcccag ccagagcatg accagcagcg agaggagcag
     2461 gtacttgttg gagaggtcgg cccagaggag caccgagaga aggatcgagc cgatgatgag
     2521 gatgccgccc atggtgggcg ttccatactt ggaggcgtgg aactcgagta ggtcggggtg
     2581 cttcaggtgg cgcagggagc ggatgaagcc cggtccgaag tagaggacgg cgaagaggct
     2641 ggtgaaggcg cccaggacca tccggacggt caggtagcgg aagacgttga agccgaacca
     2701 gaaatccttg agcaggtaaa gaagctggta aagcatcttt tcaaaaacgc cggcctgggc
     2761 cggcgcgcgg ttcgacgggg ttgaaggaag tgagtttgac ccggcccacc ggccgggagt
     2821 cctgccttga ccctattata ctacaaccgg ccgccggcgt gcgaatacgg gaagcaggcc
     2881 gttggatttc cggcggcgtt caagcaaaaa gtttacccgc cgccgttcga aagcgaagga
     2941 tggctaaggc ggacaagccc ccgggagcgg caagccgggc gattgtccac ggaaacgttt
     3001 ttgtccgtca tacccgcgga tagctggcgg atatccagac tgcccgacaa gcccgcataa
     3061 gaagttcaag ctcttcaggg tgattccggt ttctccagga caaagagctc caactccagt
     3121 ttcaactggc cgtcccgttc gttcaccgtc aggacctggc ggccgacggt gtcgaaggtg
     3181 agcaggaact cggtcttgcc cgggaccggg accgatttga agccgggcgt tacggaggtg
     3241 atttcgagct tctccggggc ggcctcgatc cggttgccga aggcgtcgcc gaggctggcc
     3301 gagacccggt attcgcggcc ccggaagagg accgccggca gaccttccag ccggattatc
     3361 tccggcctgg ccggcagcac ctcgaaggca ttcgaacggc cggtgtggcc gagaccgtcg
     3421 gccacttcga cggccgcctc ccggccggcc cgggtgatgg tgacctcctc gacccggctg
     3481 aaggcgatga tgctctcccg gaccggtttc aaggtatggc tggggtcgtt cagttccagg
     3541 cccgggaccc ggttgcccca ccggtcctcg ctccggatga agaccgtgaa gggctggccg
     3601 gcgacctggg gcgagcggat cgggtcgatc cggaaccggt ccaacggccc ggggcgcacc
     3661 gccacctcgg tttcgccctc gagttcaccc gcggcggcgc cggagatttt cacccggagt
     3721 ttccgcttgc cggccttgaa gaaggtcagt tccgggccgg ccggtccgga gagggagagc
     3781 tccgccggca gtttggcctc cgggtcgtcg gaggcgaggg aggctttccc ggtgaaggcg
     3841 gccgggtttc cgaaccggtc ggtcacctgg atctccagct tgaccggctc gccggcccgg
     3901 gccgggccgg ccggggtaag gcggaccttt tcggccgggc cggccgtgac cgagaagacg
     3961 ggcgaggcgc cgctgttctt ttcccccagc aggaccagcc ggttttcggc ggccggccgg
     4021 gtgaagttga tcggtccctg ccaggtcccc ttctggataa ggatggtctc gggccggagg
     4081 ctctccgcga ggtccaggac ccagaccggg gcgtcgagcg ggtagggagc gcccgattcg
     4141 tcaacgacgc tgattttaac ctgctgggcc ccggcctcca ggctcgggcc gaaagcatcg
     4201 aagcggaagc gcagcccggc gcccggtgcg gccggcgcgg ccggagtccc gggtgcggcc
     4261 ggcgccgatg gcgtttccgg tccggccggg gttccgggcg ccggcggagt ggccggcgcg
     4321 gccgggattc ccggtgcggc cggcgtttcc ggggcggccg gtgcggccgg agtgctccgg
     4381 acctccggta tgggcggccg ccgccttatc gtggccgaac cgtccggggc gagagtgccg
     4441 tagccgccgg tgtggggcgg cgggatgtcg gaacggaccg cggtcgcctc ggtcatgatc
     4501 caggtcccct gcggcaggga gctcaggtcg ttccggttga ccaggtcatt ccagagatag
     4561 ttggtgccgc tcttgttgag ctcctccggg atggagggac agtagaagat ggccgggttg
     4621 cttacgtact tcttgagcac gttgtggatg ccgcggggat cgctggccga cgacgggaag
     4681 aaacgggcct ccggcagggc gccgtaatcc atggcgcaga tgttcagggc tatgtatacc
     4741 tggcgcaggt tgttgaggca gttggcctcc ttgcccttct ttaccgcctg gtaggcggtc
     4801 atcgagaccg aggtgaccag gctggtcacc atggcgatct cgacgtaggt gaaaccccac
     4861 cgtcttttcc gttccatccg gtctccctgg ttcgggctgt ccttcaacgg ccgggcttct
     4921 ccgcgccttc ggccgggcgg aagaagagcc agttgcgctg gcggtccgcc ttgaagaaga
     4981 gcaggatata gacgccgcgg agtttttcac cccgcagttc gaagacgatc ttttcgggcc
     5041 ggcgctcgat cagccggtag gggccgcggt cccagatttc gacccggccg gccccgtact
     5101 cgccttccgg tatcaggccc tcgaaatcgc cgtaatcgag cggatggtcc ggcacctcga
     5161 ccgccagccg cttctcaccc gccgcttccg gcggggcctt gggcaccgcc caggacttga
     5221 gcacgccgtc cagctcgagc cggaagtcga agtggtggcg gcgggcgaag tgctcgtgca
     5281 ccacgaatct caactctctt gcttcagcag gcttgatggg cataggtctt ccaggcagca
     5341 gccggcgcag gcgggtttcc gggcctggca gacccaccgg ccgtgcagga tgagccggaa
     5401 ggcgaaattc ccccagtcgc gctgaggcag gatggccatc aggtcggtct cgatcttgac
     5461 cgggtcggtc tcccgggtca ggcccagccg ctggctcacc cgggcgacat gggtgtcgac
     5521 caccacgccc tcgttctttc tgaagacctg ggagaggacg acattggccg tcttgcgcgc
     5581 caccccgggc agccggacca gctcctccat gttccggggc accttgccgc cgtggcggtt
     5641 gatcaggatg ccggacatgc gcttgagata accggccttg ttccggtaga agttgatcga
     5701 gccgatcagt ttttcgattt cggcctggga ggcgcggttg aaggcggccg gcccggccat
     5761 ccgggcgaag aggttggggg tggcccggtt gacctgctcg tcggtgctct gggccgagag
     5821 gatggtggcg accagcagtt gaaagggcgt ctggaacttg agggcggtcc gggt
//
LOCUS       DAOQYN010000120         5847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_13958_length_5847_cov_4.517956, whole genome shotgun sequence.
ACCESSION   DAOQYN010000120 DAOQYN010000000
VERSION     DAOQYN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13958_length_5847_cov_4.517956"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /locus_tag="PKN80_05780"
     CDS             complement(<1..1867)
                     /locus_tag="PKN80_05780"
                     /inference="COORDINATES: protein motif:HMM:NF039200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HNQ35557.1"
                     /translation="MKRRPILALLALAALAFLPGPGRADETGPGKSGSLPVDYQNFLS
                     RNDIVLLSPDRHAWDGLPLGNGRIGARTWQPDELMFQLNARGGALTLVRLAVGAEPGL
                     RSGLEEYSQRLSLYDATVTTRSRHADGQLEIRAWMAATTDALVVQVNDNRAGAATHYV
                     DVEAWRPGVEVITGPDYVLLREKAGEIAWALRISIPGGRVTSRSESPRVVRIATNTRQ
                     TEITALYVEAEPEADASARAEQAAESFTKRGISGFRREHCQWWSDFWSKSVLNLHSAD
                     GLAVYLENLWYIHLYCLGSGMRGELPMNFENGLWHSQRDIRGWGPGYWHWNEQVAVWP
                     LFAANHLELHESYRNLFRRLLPGYQAWTQSYFGTQDPEAVNFLEVVRSSAPGPYRGPK
                     EASDHPTLHVPKNRGNLILSSSLETAMQFWWAYLYTGDEAYLRNQAYPMMKATALFFL
                     EYLYRDGTGRYVMDPSDAHETFERVRNPVNDLAGIRYVFPAVIETAELLGVDTGLQAR
                     CREVLDNLAPYPIDPGTGALLPYELRPGQKLMTEMGHTGHCPSQHVYGVFPLMTLGST
                     DHGLAVRSFHEHLKKHTNQYGWVVDSLCAARLGLAEELKNLLTDHVRRFQHYRCGL"
     gene            complement(2071..4368)
                     /locus_tag="PKN80_05785"
     CDS             complement(2071..4368)
                     /locus_tag="PKN80_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNQ35558.1"
                     /translation="MHKKLRAFALAALLLLAASGARAANLIDDSSFEAGAGNGWGVAM
                     GAEPSRVSWNSYYDSTTAVDGRYSLKIPTTNRQLTMQVARSVFTIETKYYRLTPGRKY
                     TLSLYMKADKPCAVEFGLLGHGPSERDAKGRAIQKPDDRLLWQKAQLETGWKRYSVSG
                     EPAASPGGLAHLSIDYRTEDLTPINVWIDAVQLEEGELSAYAPRLPVEIGFRCPVPGN
                     IYYETEPARLKLLVYNAAGRSGTARINYSITDLFDREVEKGTLQADLAKGGKNLEIEM
                     APYIKRRGIFRLLAWVDEGNGRNGQPVEMTYSVLPPNKHLNEPFEGGLLGTDVSALRY
                     DQLQVLKRANFNWILSKKVVLWDRAEPTKGDFRFYDEEIEAARKAGVVLVGQLLWGDG
                     VAWSMKNLPAGDLRKPKVATWDETARAQFLKDVAEYTTAVVSHYKGKIKYYELTNEPY
                     FQFTPEQVGEVYRTMYQAAKKADPNCVCAINTDYRLTTDEKGEVVRGEHYYLPQTVEK
                     YGLNICDVVSAHFYINDLRWFLPFGNLLKKYGKPGWNTETGITCASFYKTLPLLAAIE
                     QGDAFWKNYLRTQVIPHADSMQKNVLLTISAGQMDKYFYYFSRFGNCSPSQPTRRAGS
                     GKENVEFDGSLRAGGVTQSIASHFFEGCQYDSRWIKDPRIEMYLFRDGTGTRGFMYAA
                     GTQPKPLLLTLAGPSRQMEFYDLMSNPVGLDKESRLPLTFLVKFFKSSLPPQQAMKAM
                     EEMKIEEGKIPAEGDVWSYDDFEMH"
     gene            4711..5334
                     /locus_tag="PKN80_05790"
     CDS             4711..5334
                     /locus_tag="PKN80_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNQ35559.1"
                     /translation="MGYISIFLVSFAIAFSGALAPGPLLAAVISGSLKSGFRAGPLLI
                     AGHALLEIVMVALLVLGLGRFINTPPVIKAISLAGAAILVYFGIGLLRRLPSISLDFS
                     AGSAGRDAGFFLQGITLSLANPYWAVWWLTIGLGLLLSAGRLGLPGLLFFFTGHILAD
                     LAWYSFVSFSLSRGRRFVSLKVYRSILAACAAVVISFGLYFGFKAFL"
     gene            5409..>5847
                     /locus_tag="PKN80_05795"
     CDS             5409..>5847
                     /locus_tag="PKN80_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35560.1"
                     /translation="MGISIGLVGLGSFGSAFAPLFKSHPRVDRIGLCDREPERVKKFA
                     DDPFFKDKFNVRDAYASLDEICRSDLDALVIITQPWLHAPQCLQAMESGKHVYSAVPI
                     SSVPDGDEILGYCDRLIETCRRTGMRYMLGETTYYRSQTMFCRR"
BASE COUNT          927 a         1764 c         1919 g         1237 t
ORIGIN      
        1 tcaggccgca gcgatagtgc tggaaacggc ggacgtgatc ggtcagcagg ttcttcaact
       61 cttcggccag ccccagccgg gcggcgcaca agctgtccac cacccagccg tactggttgg
      121 tatgtttttt gagatgttcg tgaaagctgc ggacggccag cccgtggtcg gtgctgccca
      181 gggtcatcaa ggggaaaacg ccatagacgt gttggctcgg gcaatggccg gtgtggccca
      241 tctccgtcat gagtttttgg cccggccgca gttcgtaagg cagcagcgcc ccggttccgg
      301 ggtcgatcgg gtagggggcc aggttatcca gcacttcgcg gcagcgagcc tggagtccgg
      361 tatccacgcc gagaagctcg gcggtctcga tcacggccgg gaagacgtaa cggatgccgg
      421 ccaggtcgtt gaccgggttg cgtacccgtt cgaaagtctc gtgggcgtcg gaagggtcca
      481 tgacatagcg cccggttccg tcccggtaca gatactccag gaagaagagc gcggtggcct
      541 tcatcatcgg gtaggcctgg ttccggagat aggcctcgtc cccggtatat agataggccc
      601 accagaactg catggcggtt tccaaactcg aggagaggat gaggttgccg cggtttttgg
      661 gtacgtgcag cgtcgggtgg tcactggcct ctttgggtcc ccggtagggt ccgggcgcgc
      721 tggagcggac gacctccagg aaattgaccg cttccgggtc ctgggtgccg aagtagcttt
      781 gcgtccaggc ctggtacccg ggcagcagcc gccggaaaag gttacggtac gactcgtgca
      841 gttccaggtg gttggccgcg aagagcggcc agacggccac ctgttcgttc cagtgccagt
      901 agcccggccc ccagccccgg atgtcacgct ggctgtgcca gagaccgttt tcgaaattca
      961 tgggcagctc tccgcgcatc ccgctgccca ggcagtagag gtgaatgtac cagaggtttt
     1021 ccaggtaaac ggccaggccg tcggccgagt gcaggttcag gaccgattta ctccagaagt
     1081 cgctccacca ttggcaatgt tcccggcgga accctgaaat gccgcgtttc gtaaaagatt
     1141 ccgccgcttg ttcggcccgg gcggacgcgt cggcctccgg ttcggcctcg acgtaaaggg
     1201 cggttatctc agtctggcgg gtgttggtgg cgatccggac gacccgggga gattccgacc
     1261 ggctggttac tcttccgccc gggatcgaga tgcgcagggc ccaggcgatc tcgccggcct
     1321 tttcccggag aagaacgtaa tccgggccgg taatgacctc cacgcctggc cgccaggcct
     1381 cgacatccac gtagtgtgtt gcggccccgg cccggttgtc gtttacctgg acgaccaggg
     1441 cgtcggtcgt ggccgccatc caggctcgga tctcgagttg accgtcggcg tggcggctcc
     1501 gggtggttac agtcgcgtca tacagggaga ggcgctggga gtattcttcc aggccggacc
     1561 gaaggcccgg ttcggcgccg acggccaggc ggaccagggt cagggcgccg ccgcgggcgt
     1621 tcagctgaaa catcagctcg tccggctgcc aggtgcgggc gccgatccgg ccgttgccca
     1681 gcggcaggcc gtcccaggcg tggcggtcgg gtgaaagcag gacgatgtcg ttgcgggaga
     1741 gaaagttctg gtagtcaacc ggcagtgaac cggattttcc cgggccggtc tcgtccgccc
     1801 ggccggggcc gggcaggaag gccagggcgg ccagggccag gagagccagg atcggccggc
     1861 gcttcatgtt ttttttcgac tcaggtttgt ttaagaagaa gctggtcatg aaatacgacc
     1921 agcggcggat ggccggtccg gcttaatccg acggcccggt tttttccggt tacccggagg
     1981 ttttggctgc cgtggcgtca atcttaaaag ataacccgga ccggagaccg gccggccgcc
     2041 actgcgtgcg gccggccgga cgcacgatct ttaatgcatt tcgaaatcgt cgtaggacca
     2101 gacgtcccct tcggccggta tcttgccttc ctcgatcttc atctcttcca tggccttcat
     2161 cgcctgctgg ggcggcaggc tgctcttgaa gaacttgacc aggaaggtga gcggcagccg
     2221 ggattccttg tccagcccga cgggattgct catcaggtcg taaaactcca tctgccggga
     2281 cggcccggcc agcgtaagca ggagcggctt cggctgggtc ccggccgcat acatgaagcc
     2341 ccgggttccg gtaccgtcgc ggaagaggta catctcgatc cgcgggtcct tgatccagcg
     2401 ggaatcgtac tggcatccct cgaagaagtg ggaggcgatc gactgggtga cgccgccggc
     2461 ccgcaggctg ccgtcgaact ccacgttctc cttgccgctg ccggcccgcc gggtcggctg
     2521 gctgggcgag cagtttccga accggctgaa gtagtagaaa tacttatcca tctgcccggc
     2581 cgagatggtc agcaggacgt tcttctgcat gctgtcggcg tggggaatga cctgggtgcg
     2641 caggtaattt ttccagaagg cgtctccctg ttcgatggcc gccagcaggg gcagcgtctt
     2701 gtagaaactg gcgcaggtga tgccggtctc ggtgttccag cccggcttgc cgtatttctt
     2761 gaggaggttt ccgaagggca ggaaccagcg gaggtcgttg atgtaaaagt gggccgagac
     2821 cacgtcgcag atgttcaggc cgtacttctc gaccgtctgg ggcaggtaat agtgctcgcc
     2881 ccggacgacc tcgcccttct cgtcggtcgt aagccggtag tcggtgttga tggcgcagac
     2941 gcagttcggg tcggccttct tggccgcctg gtacatggtc cggtagacct cgccgacctg
     3001 ttccggggtg aactgaaagt agggctcgtt ggtcagctcg tagtacttga tcttcccctt
     3061 gtaatggctg acgaccgccg tggtgtactc ggccacgtcc tttaggaatt gggcccgggc
     3121 cgtctcgtcc caggtggcga ccttgggctt gcggaggtcg ccggccggca ggttcttcat
     3181 ggaccaggcg acgccgtcgc cccagaggag ctggccgacc aggaccacgc ccgccttgcg
     3241 ggccgcctcg atctcctcgt cgtagaaacg gaagtcgcct ttggtcggct cggcccggtc
     3301 ccagagaacg accttcttgc tcaggatcca gttgaagttg gcccgcttga gtacctggag
     3361 ctggtcgtaa cggagcgcgg agacgtcggt gcccagcagg ccgccctcga agggctcgtt
     3421 caggtgcttg ttgggcggca ggaccgagta ggtcatttcg accggctgtc cgtttcggcc
     3481 gtttccttca tcaacccagg ccagcaggcg gaagatgccg cgtctcttga tgtagggggc
     3541 catctcgatt tcgaggttct tccccccctt ggcgagatcc gcctggagcg tccccttctc
     3601 gacctcgcgg tcaaagaggt cggtgatact gtaattgatc cgggcggtgc cgctccggcc
     3661 ggcggcgttg taaaccagca gcttgagccg ggccggttcc gtctcgtagt agatgttgcc
     3721 gggcaccggg cagcggaaac cgatctcgac cggtaagcgc ggcgcgtagg cgctcagctc
     3781 gccttcctcc agctgcacgg cgtcgatcca gacattgatg ggagtgaggt cctcggtgcg
     3841 gtaatcaatg ctcaggtggg ccagtccgcc cggggaggcg gccggttcgc cgctgacgct
     3901 gtagcgcttc cagccggtct ccagctgggc cttctgccag agcaggcggt cgtccggctt
     3961 ctggatggcg cgccccttgg cgtcccgttc cgaaggaccg tggccgagca ggccgaactc
     4021 gacggcgcag ggcttgtcgg ccttcatgta cagcgagagg gtgtacttgc ggccgggggt
     4081 caggcggtag tacttggttt cgatcgtgaa gaccgaccgg gcgacctgca tggtcagctg
     4141 ccggttggtg gttggaatct tcaggctgta cctgccgtcg acggccgtgg tggagtcgta
     4201 gtaggagttc cagctgacgc gcgacggttc ggcccccatg gccacgcccc agccgttgcc
     4261 ggcgccggct tcgaagctgg agtcgtcgat caggttggcc gcccgggcgc cggaagcggc
     4321 caggagcagc agggcggcaa gtgcgaaggc tctcaatttt ttgtgcatcg tctttctcct
     4381 ttgactgcgg tttgaccggt cttgttaata aggaaaggca acctttttct gcggcgccgg
     4441 gccggtcgag gaaaggtccc ggtcgccgcc tgggagcatt aatatggttg aatcagaata
     4501 tattattgtt tacttgcctg tcaagcaaaa ttccggggaa acggtttgca atcaagccgt
     4561 tgccaaaggt ccctgtttct aaccggtccc gatctcaccg tcttccattt tctcggccga
     4621 gagtgtaaac taaaaatggc cggctggttt gatcattgcc gagcccgagg ttggtcctgg
     4681 ctcgaacgct tccggttttc aaaataaacc atgggctata tttccatttt ccttgtctcc
     4741 ttcgcgatcg ccttttccgg cgccctggcg cccggtccgc tgctggcggc ggtcatctcc
     4801 gggagcctga aaagcggctt ccgggctggg ccgctgctca ttgccggcca cgccctgctg
     4861 gagatcgtga tggtggccct cctggtcctg ggcctgggcc gtttcatcaa taccccgccg
     4921 gtcatcaagg ccatctccct ggccggggcg gccattctcg tctatttcgg catcggactg
     4981 ctgcgccggc tcccttcgat ttccctcgat ttttccgccg gttcggccgg gcgggacgcc
     5041 ggtttcttcc tgcaggggat cactctcagc ctggccaatc cttactgggc ggtctggtgg
     5101 ctcacgatcg gcctgggtct gcttctttcg gccggccggc tcggccttcc cggcctcctt
     5161 ttctttttta ccggccacat cctggccgac ctggcctggt acagcttcgt gtccttcagc
     5221 ctgagtcggg gtcgccggtt tgtctctctc aaggtttacc gctcgatcct ggccgcctgc
     5281 gccgcggtcg tgatctcttt cgggctctat ttcgggttca aagccttttt gtaagccggc
     5341 ccgagattcg gagccggctt cgggaaatct gaacgcaggg gtttaaacaa cctgaaaagg
     5401 agtgaagaat gggaatcagc atcggtctgg tgggtttggg ttccttcggt tccgccttcg
     5461 cgcccctctt caagagccat ccgcgggttg accggatcgg cctctgcgac cgggagccgg
     5521 aacgggtgaa aaagtttgcc gatgacccgt ttttcaagga caagttcaac gtccgcgacg
     5581 cctacgcctc gctggatgag atctgccgga gtgatcttga cgctctggtg atcatcaccc
     5641 agccctggtt gcacgccccc cagtgcctcc aggctatgga gtccggcaag cacgtttaca
     5701 gcgcggtgcc gatcagttcc gtccccgacg gcgacgagat cctcggctat tgcgaccggc
     5761 tgatcgagac ctgccggcgg accggcatgc gctacatgct gggcgagacg acctactacc
     5821 gttcgcagac gatgttctgc cggcgcc
//
LOCUS       DAOQYN010000121         5830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_14021_length_5830_cov_5.027706, whole genome shotgun sequence.
ACCESSION   DAOQYN010000121 DAOQYN010000000
VERSION     DAOQYN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14021_length_5830_cov_5.027706"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2716)
                     /gene="ileS"
                     /locus_tag="PKN80_05800"
     CDS             complement(<1..2716)
                     /gene="ileS"
                     /locus_tag="PKN80_05800"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNQ35561.1"
                     /translation="MDYRKTVNLPRTDFPMRANLVGREPEILRFWSERRTFELMAERR
                     SRRRFVLHDGPPYANGHIHLGTAFNKILKDMVVRSQHKSGAEVPFRPGWDCHGMPIEH
                     QILGRSGRRKGEVDQKVFRREAADYARRFLDIQRKEFQRLGVYGDWSHPYLTLAPEYE
                     AREVRIFARLALDGYIYRGKRPIYWCCNCETALAEAEIEYQDLPSDSIYLLFPVASDP
                     DRVFKNPANVYFLVWTTTPWTLPANVALIVHPGLDYRLVEFEGKELLLAENLVETVFG
                     RLGKKAVRLETRLKGDRLTRLTARHPFLDRPSVVVSADFVSAEDGSGVVHSAPGHGEE
                     DFLVGREHNLPILSPVDETGRFTAEVADWAGQSVWEANPKIIERLRERGRLLLAETVT
                     HSYPTCWRCKKPVIFRATEQWFLNIDHQDLRRRLKAAAADISFLPSESRNRLEGMLET
                     RPDWCLSRQRHWGVPVPILFCRACRKPLMEQAVFDRVAAVFEAEGSDAWFEKGPDYFV
                     PEGVACGCGGRDFVQEKDILDVWFDSGTSWAGVLEPDGLHPADLYLEGSDQHRGWFQT
                     SLITSVATRGVPPFRAVLTHGFLVDEAGRKMSKSLGNVITPADVLKEFGADILRLWAA
                     GQDYRNDAKASLSLLTQVSGQYRTLRNTVRFILGNLADFDPAADALERDKLRPVDRWI
                     LAELAELSAGVVDQYRQFSLHRASAQLYDFCNLTLSAFYLDILKDRLYTCAPDSDARR
                     SAQTALYLVVRQLINLLAPVLVFTCEEAWRYLPHRPGDPESVHLLDFEDLDGYRDQPL
                     RDDFGILLEARRQVLREVEPVRAQGLIGSSLQARVEIAAAADILPVLRRFEAALPELL
                     IVSEVILVESPDPALTRISVRPTAWSKCPRCWVHSPDLGAAAAESEVCP"
     gene            complement(2802..3647)
                     /locus_tag="PKN80_05805"
     CDS             complement(2802..3647)
                     /locus_tag="PKN80_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNQ35562.1"
                     /translation="MGSQTKTIFLLGGLTALLVLIGSFFGRGWTYGALAFALLLNFFT
                     YWFSDRIVLKMYRAQPVTAADSPVLYDTVRQLSQVAGLPMPKVYVYPAANPNAFATGR
                     DPAHAAVAVSTGLMEILSPEELKGVLGHELAHIKDRDILIATCAATLAAAITFLARLL
                     QWGAGFFAGGDNRERGSNPLAMLAMVILAPLAALLIQMAVSRGREYLADDEGARFAGN
                     PAFLANALRKLDSVGRRNPMVGANPATSHLFIVKPFSARGLMALFSTHPPIEARIARL
                     EGRIT"
     gene            3765..4181
                     /locus_tag="PKN80_05810"
     CDS             3765..4181
                     /locus_tag="PKN80_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35563.1"
                     /translation="MPKTDFPGFLRKVKPEITLVEGLLRQPLSEEPEGLIADLLKIEV
                     WYARIQTILAFAEGYLDLAEREKLPTKETRTELDREKELASRVAAERLLRDRASGLVE
                     ALKTRIMLGQTLLNYYRDVHVSDRPRTRKPSGPDGF"
     gene            4274..5131
                     /locus_tag="PKN80_05815"
     CDS             4274..5131
                     /locus_tag="PKN80_05815"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ35564.1"
                     /translation="MYQGPIINIHGHLRHGQDFDLRLAAWRKWGVRKFVCLCLHPRWS
                     LKGYYTNQDFLQLADRYREVIVGFGAVNLVAGQVDRPEAVDRLKEQGFAGIKCEDNSF
                     PYNHEIYWPLYARMEALGMPALFHTGNVAPLELNDGVNHEGRDGIDAENMRPYRLDRV
                     ARAFPKLRIIGAHLGLPHPFEAINLAQTRPNVFFDFSGGGGSRSHVNQVLAALSPRLP
                     GARLDDPADNFALPLFEKKLLFGTDNPEPEVWVPASERIMDALAIPESSRRRFYHDTA
                     AEILGPAAE"
BASE COUNT         1077 a         1863 c         1965 g          925 t
ORIGIN      
        1 tcggacagac ctcggactcg gccgcggcgg cgcccaggtc cgggctgtga acccagcagc
       61 gcgggcactt ggaccaggcg gtcgggcgaa ccgagatccg ggtcaaggcc ggatccggcg
      121 actcgaccag gatgacctcg gagacgatga gaagctccgg cagggcggcc tcgaaacggc
      181 gcagcaccgg cagaatgtcg gccgcggcgg cgatctccac ccgggcctgc aggctgctgc
      241 cgatcaggcc ctgggcccgg accggctcga cctcgcgcag gacctggcgc cgcgcttcca
      301 gcaggattcc gaagtcgtcc cgaagcggct ggtcccggta cccgtcaagg tcttcaaaat
      361 caagcaggtg gacgctctcc ggatcgcccg gccggtgcgg caggtaacgc caggcctcct
      421 cgcaggtaaa gaccaggacc ggggccagca ggttgatcag ctggcgcacg accaggtaaa
      481 gggcggtctg ggcgctgcgg cgagcgtccg aatccggagc gcaggtatag aggcggtcct
      541 taaggatgtc gaggtagaag gcgctgagcg tcaggttgca gaaatcgtag agctgggccg
      601 aggcccggtg cagcgaaaac tggcggtact ggtcgacgac tccggcggac agctcggcca
      661 gttcggccag gatccagcgg tcgaccggcc ggagcttgtc ccgctcgagg gcgtcggcgg
      721 ccggatcgaa atcggccagg ttgcccagga tgaagcgcac ggtgttgcgc agggtccggt
      781 actggccgga gacctgggtc agcagcgaca gggaggcctt ggcatcgttg cggtaatcct
      841 gcccggccgc ccagaggcgc aggatgtcgg ccccgaactc cttgagcacg tcggccgggg
      901 tgatcacgtt gcccagcgac ttgctcatct tgcggccggc ctcgtcaacc aggaagccgt
      961 gggtgaggac cgcccggaag ggcggcacgc cccgggtggc gaccgaggtg atcaggctgg
     1021 tctggaacca gccgcggtgc tggtcgctcc cttccaggta gaggtcggcc gggtggaggc
     1081 cgtccggttc cagcaccccg gcccaggagg tcccggagtc gaaccagacg tccaggatgt
     1141 ccttctcctg gacaaagtcc cggccgccgc agccgcaggc aactccctcg ggcacgaagt
     1201 aatcgggccc cttttcaaac caggcatccg agccctcggc ttcgaagacc gcggcgaccc
     1261 ggtcgaagac cgcctgctcc atcagcggtt tccggcaggc gcggcagaag aggatcggca
     1321 ccggcacccc ccagtggcgc tggcgggaga ggcaccagtc gggccgggtc tccagcatgc
     1381 cttccagccg gttccggctt tcggagggca ggaaggaaat gtcggcggcg gccgccttga
     1441 gccgccggcg caggtcctgg tggtcaatgt tcaggaacca ctgctcggtg gcccggaaga
     1501 tgaccggctt cttgcagcgc cagcaggtcg gataactgtg ggtcaccgtc tcggccagca
     1561 ggagccggcc gcgttcgcgg aggcgttcga tgatcttcgg gttggcctcc cagacggact
     1621 ggccggccca gtcggccacc tcggcggtaa agcggcccgt ctcgtccacc ggcgaaagga
     1681 tcggcaggtt atgctcgcgc ccgaccagga agtcctcctc gccgtggccg ggggcgctgt
     1741 gaactacgcc gctgccgtcc tcggcggaga cgaagtcggc cgaaaccacg acggacggcc
     1801 ggtccagaaa gggatggcgg gcagtcagcc gagtcagccg gtcgcccttg agccgggttt
     1861 cgagccggac cgccttcttg cccagccggc cgaagaccgt ctccaccagg ttctcggcca
     1921 ggagcagctc cttcccctcg aactcaacca ggcggtagtc aaggcccggg tgcacgatca
     1981 gggccacgtt ggccggcagg gtccagggcg tggtcgtcca gaccaggaag tagacgttgg
     2041 ccgggttctt gaagacccgg tccgggtcgg atgccaccgg gaagagcagg tagatggagt
     2101 cggacgggag gtcctggtac tcgatctcgg cctcggccag ggcggtctcg cagttgcagc
     2161 accagtagat gggccgcttg ccgcggtaga tgtagccgtc cagggccagc cgggcaaaaa
     2221 tccggacctc gcgggcctcg tactcggggg ccagggtcag gtaggggtgg gaccagtcgc
     2281 cgtaaacgcc cagccgctgg aactccttgc gctgaatatc caggaagcgg cgcgcgtaat
     2341 cggccgcctc gcgccggaag accttctggt caacctcgcc cttgcgccgg ccggaacggc
     2401 ccaggatctg gtgctcgatc ggcataccgt ggcagtccca gccgggccgg aagggaacct
     2461 cggcgccgct cttgtgctgg ctgcgcacca ccatgtcctt gaggatcttg ttgaaggcgg
     2521 tccccaggtg gatgtgaccg ttggcgtaag gcgggccgtc gtgaagcacg aagcggcgcc
     2581 tggagcggcg ttcggccatc agctcgaagg tccggcgctc cgaccagaag cggaggatct
     2641 ccggctcccg gccgaccagg ttggcgcgca tcggaaagtc ggtccggggc aggttaaccg
     2701 ttttgcggta atccatggtc tctttatccc ctaatagtaa attccgctga gtaagggccg
     2761 cgtcgccttt tcggcggacg cgaaaaggca aggcggcgga atcaggtaat tctgccttcc
     2821 agccgggcga tacgcgcttc gatcggaggg tgggtggaga agagcgccat caatccgcgg
     2881 gcggaaaagg gtttgacgat aaagaggtgg ctggtggccg ggttggcgcc gaccatcgga
     2941 ttccggcggc cgacgctgtc cagcttacgc agggcgttgg ccaggaaggc ggggttgccg
     3001 gcaaagcggg ccccctcgtc gtcggccagg tactcgcggc cccggctgac ggccatctgg
     3061 ataagcaggg cggccagcgg cgccaggatg accatggcca gcatggccag cgggttgctg
     3121 ccgcgttccc ggttgtcgcc gccggcaaag aaacccgccc cccactggag caggcgcgcc
     3181 aggaaggtaa tggcggccgc cagggtggcg gcgcaggtgg cgatcaggat gtcccggtcc
     3241 ttgatgtggg ccagttcgtg gccgagcacg cccttgagct cctcgggact gaggatctcc
     3301 atcaggccgg tggagaccgc gacggccgcg tgggcgggat cgcggccggt ggcaaaggcg
     3361 ttcgggttgg ccgccgggta gacgtaaacc ttcggcatcg gcagcccggc cacctgggag
     3421 agctggcgga cggtgtcgta aagaaccggc gaatcggcgg cggtgaccgg ctgggcccgg
     3481 tacatcttca gcacgatccg gtcgctgaac cagtaggtga agaagttgag cagcagggcg
     3541 aaggccaggg cgccgtaagt ccagccccgg ccgaagaagc tgccgataag gaccagcagg
     3601 gcggtcaacc cgcccagcag gaagatggtc ttggtctggc tgcccatttt ctttgcctct
     3661 tggccgcttc tgtgttatta ttcttaacaa aaaacggccg gtttctttaa caggatgaac
     3721 cttaccggcc gcccggttct gtccgccaaa tattataaca taagatgccc aagaccgact
     3781 tccccggctt tttgcgcaag gtcaagcccg aaataaccct ggtggaaggg ctgctgcgcc
     3841 agcccctctc cgaggagccg gaggggctga tcgccgacct gctgaagatc gaggtctggt
     3901 acgcccggat ccagaccatc ctggccttcg ccgagggata cctggatctg gccgagcggg
     3961 agaaactgcc caccaaggag acccggaccg agctcgaccg ggagaaggag ctggcttcgc
     4021 gggtggcggc cgaacggctg ctgcgcgacc gggccagcgg cctggtggag gcactcaaga
     4081 cccgcatcat gctcggccag accctgctga actactaccg cgacgtccac gtttccgacc
     4141 ggccccggac cagaaaaccg tcgggaccgg acggtttctg ataaacggcc gcggttgaaa
     4201 agaaacccgg ccccgccacc gcaccgcaaa aacccggggc gccgacgccc accgaaaaga
     4261 aggaaggtga aaaatgtacc agggcccgat catcaacatt cacggccacc tgcgccacgg
     4321 acaggacttc gacctccggc tggccgcctg gcgcaagtgg ggcgtgcgca agttcgtctg
     4381 cctctgcctg cacccccgct ggagcctcaa gggatactac accaaccagg acttccttca
     4441 gctggccgac cggtaccggg aagtgatcgt cggcttcggc gcggtcaacc tggtcgccgg
     4501 ccaggtggac cggccggagg cggttgaccg gctcaaggag cagggctttg ccggaatcaa
     4561 gtgcgaggat aactcctttc cctacaacca cgagatttac tggcccctct acgcccggat
     4621 ggaggcgctt ggaatgccgg ccctctttca caccggcaac gtggcgccgc tggaattgaa
     4681 cgacggcgta aaccacgaag gtcgcgacgg gatcgacgcc gagaacatgc gcccctaccg
     4741 actggaccgg gtcgcccggg ccttcccgaa attgaggatt atcggcgccc acctgggcct
     4801 gccgcacccc tttgaggcga tcaacctcgc ccagacccgg cccaatgttt tcttcgactt
     4861 ctccggcggc ggcggcagcc gcagccatgt caaccaggtg ctggcggcgc tcagcccgag
     4921 gctgcccgga gcgcgactgg acgatccggc cgacaacttc gccctgcccc tcttcgaaaa
     4981 aaaactgctc ttcggcaccg acaacccgga gccggaggtc tgggtgccgg ccagcgaacg
     5041 gattatggac gcgctggcca tacctgaaag cagccgccgt cgcttctatc acgataccgc
     5101 cgctgaaatt ctcggcccgg ccgcagagta gtttcgtcgc accggcgcta aaagacaaac
     5161 tctggcggac tgcccgcgaa aacagccggc aaaaagatcg ccccggtttt tcccgcttaa
     5221 caaatccggt ttcccccggt acaataagtt tggaggaagg agggagaggc aggtgaacga
     5281 aacagagacc gatcccgaga aagttgaggt ggctcctttc gggcggcttc catcttcctg
     5341 aaggccttct tccagagagg gttgggcgta gtacaggttt aagcgtgacc cgaccctctc
     5401 tttttttgtg ggcggtgggc ggggatccgg gcgatcttgg tgacggcgtc gcaagctacg
     5461 ccccgaaaga tgcccggggc tcgctttcag gggaagaagc ttccctcccc tgattccccc
     5521 tccccggaca cgactacgag aaggggttgc caccccctct ctcaccccca cgccaccggt
     5581 aaaaacgtcc ggcggcggag cgcccgagcc aagtccggac cctcggcgcc ccgaaacaaa
     5641 aaacacacaa acctggattc tcgcctacta tccgcgggaa tgacaggcaa aaaacgtggc
     5701 tgttaatcac aacctccgcc atccttgtct ttgcttttcg tctgttcccc ttaagcggcg
     5761 gtcttcgggg agcgccgctt gcgtagcgat ggctacaaaa caagccgggc gaggcaatga
     5821 gtagccgggg
//
LOCUS       DAOQYN010000122         5816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_14079_length_5816_cov_4.646068, whole genome shotgun sequence.
ACCESSION   DAOQYN010000122 DAOQYN010000000
VERSION     DAOQYN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14079_length_5816_cov_4.646068"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKN80_05820"
     CDS             <1..552
                     /locus_tag="PKN80_05820"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNQ35565.1"
                     /translation="VLLRAQRNARQVACLNNLRHLGAAFQKYLADWDGRPPCGDWDAA
                     GHRRGAWAVQLAPYLDIQLNGDPAASAERINSCGHFFCPTSTASHRHYGLNHPGWRDL
                     AGRNLFRSRRKLDSLVYLGEVAGPPWSIGGRTTENQVDYNRHHGRASFLFADGHVATG
                     TPEEFGFHYKSVGPERVYWTGLE"
     gene            682..1641
                     /locus_tag="PKN80_05825"
     CDS             682..1641
                     /locus_tag="PKN80_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35566.1"
                     /translation="MKKKPEISMYYLDGDPFVWQPKQLRADFEAMRRIGADSVCISVL
                     ESHTSDYPGKVALARSAGLKVYAIPSRVGGFFAGWPKVASLFAARHPEYLVLTRDGRF
                     QDATAGSVCCPNQPAFRAWFLEWLEKLLDESGVDGVVYDEPKEAHVPCWCPACRKLCP
                     DQDEAARVRFREASVAGLLGEACRLVKRSRPDRTNILFVMPDATGHFKTRILEQPELD
                     YFGNDGPVCHQGEPEQTHQKTSLFDSVPAIFRLADQAGKKRFTLVETFSIHSGAMAEL
                     ARNLPRVAEWQADLYAFYFYGTGNQEPERVMELSAEAIARIKG"
     gene            1655..4699
                     /locus_tag="PKN80_05830"
     CDS             1655..4699
                     /locus_tag="PKN80_05830"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNQ35567.1"
                     /translation="MSQHHDITPAEFSFDRFRRPETRFRGLPFWSLNDRLEPEELRRQ
                     VREFRRGGLGGFFLHARAGLETPYLGRAWFDGLTAAVDEARKEGLTAWLYDEDRWPSG
                     FAGGRVLEGAPELRVKTLICSPSPPVEARQRLVKKAETAGGPLYFYQDYDPADHPRFN
                     YSGYVDLLNPRTLDRFIRTTHRAYRRRLGGEFGRSVPGLFTDEPHWRGMLYGPGIPWT
                     ENFPGYFLRKRGYDIREHLPALFRPLAGAGRVRLDFWSAAAELFAENFSRRLYRWCER
                     NRLLFTGHYNGEEPLLVQFLNQAFMFHYQYQHYPGVDHLGWNIKPLVTMKQAASVANQ
                     LGKPRVLSELYAGGGWDFGPAGLKWVGDWEHALGVNLRCQHLSHYSLRGHRKLDYPPS
                     LFFQQPWWPHHHRLEDYFARLEYLLTRGRYHAEILLLHPSTSAFASFQPAASYAGLFR
                     ANRRLVGIDRSFNALSRRLCELHYGHDYGDETLLAEHGRVEGGRLRVGQAAYRLVILP
                     PLFNLMEGTLKLLEAFRESGGRVLALEPRPRLLSGRPSKRLAAFFGRPGVEMLDKIEL
                     LTDLLNGLAAPDIIINDGRGGGTPSIYAHRRDCGRSQVYFLVNLDRFRGGRYRVGLAG
                     RKKPLEWDAAEGTARRLPARREGKGWQLDLEFAPAQSRLLVINGPEPARVKAAETGTG
                     PKPVRRPLKNEWEFRRLDPNALVLDYCRYRVNSGAWSPRRPVWQAEAELRQALKAGAW
                     DANSGAQPWLNRRRLGRLKILATVELAFDFQVKQPPKPKTEIFLVMETPERFQIRFNG
                     RPLRRPAHPGWWVDPAFRKLPAAGRLRPGRNEIILRTGFRSDTELQNLFITGDFAVAG
                     PGRRRFRLTAAEPERLRSGAWTAQGYPFFAGRLAAAQEFELKENLGRVWLELENPAAA
                     VYQVRINGRSAGTLLWPPYRLEITGLARPGRNRLELEAVNSLRNLLGPHHYRIPAGRR
                     FDQERELVGPGTFRNEKKWTETYQLVSDGLPLASLLFPRRET"
     gene            4696..>5816
                     /locus_tag="PKN80_05835"
     CDS             4696..>5816
                     /locus_tag="PKN80_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35568.1"
                     /translation="MRKFRITIGLAALIAAALPGIVRAQVAPGVNWLKNPGFEEVGTG
                     GSLPGGYTVGGNPKVEWVKEAHSGNYALALAPMQASGNWFRFPINVPGGPPRKFVFSF
                     WTRTEFDPATGDSPSFYLHMSTPGFVGAGGSFPRTRFRYLGEWTHIGGLVTSDINTIG
                     WFRFILYTTKPGLRIFLDDLFFAEVTGLPEAEIQKLLAGTTLTPKMELASAVKEETDY
                     QPGNKFANSSFELETGCGWGIFGGGKLVPVAGGLEGGRAMPAERLWYQRLKLRPYRRH
                     TFSGYFKAAAGSSANLRVEFLNGYSDGYRTKQAAISQDFKVTGDWQRYNFSFVPPANP
                     SGNTYTLRLSGRVLADNLALEEGDLRPYQPARTLEHGIST"
BASE COUNT          976 a         2119 c         1814 g          907 t
ORIGIN      
        1 gtcctgctcc gggcgcagcg caatgcccgc caggtcgcct gtctcaacaa cctgcgtcac
       61 ctgggcgccg ccttccagaa gtacctggcc gactgggatg gccggcctcc ctgcggcgac
      121 tgggatgccg ccggccaccg gcgcggcgcc tgggccgtcc agctggcccc ctatctcgac
      181 atccagctga atggagatcc cgcggcgtcg gccgaacgga tcaattcctg cggccacttc
      241 ttctgcccga catcaaccgc ttcccaccgc cactacggcc tcaaccaccc gggctggcgg
      301 gacctggccg gccgcaacct ctttcgaagc cgccgcaagc tcgattccct ggtctacctg
      361 ggcgaagtgg ccggcccgcc ctggagcatc ggcggccgga cgaccgaaaa ccaggtcgac
      421 tacaaccgcc atcacggacg ggccagtttt ctcttcgccg acggacacgt ggcgaccggc
      481 accccggaag agtttggctt ccactacaag tcggtcggac cggaacgggt ttactggacc
      541 ggcctcgaat aaccggcgta ttcacgcccc cttactttac gtccccggat tgtcccgcgt
      601 tgacccgcgg tgctataatt tccacgaaaa gaagttttta ccgaacccgg ccgcgctccc
      661 gtcaaccaaa aaggaataaa aatgaaaaag aagcccgaaa tttcaatgta ctaccttgac
      721 ggcgacccct tcgtctggca gccgaagcag ctccgggccg acttcgaagc gatgcgccgg
      781 atcggcgccg acagcgtctg catcagcgtc ctggagagcc acacctccga ttacccgggc
      841 aaggtggcgc tggcccggtc ggccgggttg aaggtttacg cgatccccag ccgggtcggc
      901 gggttcttcg ccggctggcc caaggtggcc agcctcttcg ccgcccgcca cccggaatac
      961 ctggtcctca cccgggacgg ccgcttccag gacgccaccg ccggcagcgt ctgctgccca
     1021 aaccagccgg ccttccgggc ctggttcctg gagtggctgg agaaactgct ggacgagtcc
     1081 ggggtggatg gagtggtcta cgacgaaccc aaggaggccc atgtcccctg ctggtgcccg
     1141 gcctgccgca aactatgccc ggaccaggac gaggcggccc gggtccggtt ccgggaggcc
     1201 tcggtggccg gcctgctggg cgaggcctgc cggctggtga agcggtcccg gccggaccgg
     1261 accaacattc tcttcgtgat gcccgacgcc accggccact tcaagacccg gatcctcgag
     1321 cagccggaac tcgactattt cggcaacgac ggcccggtct gccaccaggg cgagccggag
     1381 cagacgcatc agaagaccag cctcttcgat tcagtcccgg ccatcttccg gctggccgac
     1441 caggccggca agaaacgctt caccctggtc gaaacctttt cgatccactc cggggcgatg
     1501 gcggaactgg cccggaacct gccccgggtg gccgaatggc aggccgatct ctatgccttc
     1561 tacttctacg gcaccggcaa ccaggagccg gaaagggtga tggaactgag cgccgaagcc
     1621 atcgcccgga tcaagggctg acgcccgcca agtaatgtca caacatcacg acatcacacc
     1681 agccgaattt agttttgacc ggttccggcg gcccgaaacg cgcttccgtg gcctgccctt
     1741 ctggtcgctc aacgaccggc tggagccgga agagctgcgg cgccaggtcc gggaattccg
     1801 gcgcgggggc ctgggcggct tcttcctgca cgcccgggcc ggcctggaga cgccctacct
     1861 gggccgggcc tggttcgacg gcctgacggc cgcggtcgac gaggcccgga aagagggact
     1921 gaccgcctgg ctctacgacg aggaccggtg gccgagcggc ttcgccggcg gccgggtgct
     1981 ggaaggggct ccggaattgc gggtcaagac cctgatctgc tctccaagcc ccccggtcga
     2041 agcgcgccag cggctggtaa aaaaagcgga gacggccggc ggaccgctct acttctacca
     2101 ggattacgac ccggccgacc atccccgttt caactactcc gggtatgtcg accttctcaa
     2161 cccccggacc ctggaccgct tcatccggac cacccaccgg gcctaccggc gccgcctcgg
     2221 cggggaattc ggccggagcg tacccggcct cttcaccgac gagccgcact ggcgcggcat
     2281 gctctacggg ccggggatcc cctggaccga aaacttcccg gggtattttc tccggaagcg
     2341 gggttacgac atccgggagc acctgccggc cctcttccgc ccgctggccg gcgccggccg
     2401 ggtccgcctc gacttctggt cggccgccgc cgaactcttc gccgaaaact tctcccgccg
     2461 tctttaccgc tggtgcgaac ggaaccgcct gctcttcacc ggccattaca acggcgagga
     2521 gccgctcctg gtccagttcc tcaaccaggc cttcatgttc cactaccagt accagcacta
     2581 tcccggcgtc gaccacctgg gctggaacat caagccgctg gtgaccatga agcaggcggc
     2641 cagcgtggcc aaccagctgg gcaaaccgcg ggtcctctcc gagctctacg ccggcggcgg
     2701 ctgggacttc ggaccggccg gcctgaaatg ggtgggcgac tgggagcacg cgctgggcgt
     2761 caacctccgc tgccagcacc tcagtcacta ctccctgcgc ggccaccgca agctggatta
     2821 cccgccctcc ctcttcttcc agcagccctg gtggccccat caccatcgcc tggaggatta
     2881 cttcgcccgg ctggaatacc tgctgacccg gggccggtac catgccgaga tcctgctgct
     2941 ccatccctcg accagcgcct tcgcctcctt ccagccggcc gccagctacg ccggactctt
     3001 ccgggccaac cgccgcctgg tcgggatcga ccgctccttc aacgcactct cccgccgcct
     3061 ctgcgaactc cattacggcc atgattacgg ggacgagacg ctcctggccg aacacggccg
     3121 ggtggaaggc ggccggctgc gggtgggcca agccgcctac cggctggtca tcctgccgcc
     3181 gctcttcaac ctgatggaag ggactctgaa actcctggag gccttccggg aatccggcgg
     3241 ccgggtgctg gccctggagc cccggccccg gctcctctcc ggccgcccat cgaaacggct
     3301 ggccgccttc ttcggccgcc ccggcgtcga gatgctggac aaaatcgaac ttctgacgga
     3361 tctcttaaac ggcctggccg cgccggacat cataattaac gacggccggg gcggcgggac
     3421 tccgtccatt tacgcgcacc ggcgggactg cgggcgaagc caggtttact tcctggtcaa
     3481 cctggaccgc ttccgcggcg gccgctaccg ggtcgggctg gccggccgga agaagcccct
     3541 ggaatgggac gcggccgaag gcacggcccg ccggttgccg gcgcggcggg aagggaaagg
     3601 ctggcagctg gaccttgaat tcgccccggc ccagagccgc ctcctggtca tcaacggacc
     3661 ggaaccggcc cgggtcaagg cggccgaaac cgggaccggc ccaaagccgg tccgccggcc
     3721 gctgaaaaat gaatgggaat tccggcgcct cgatcccaac gccctggtgc tcgactactg
     3781 ccgctaccgg gtgaacagcg gcgcctggag cccgcggcgg ccggtctggc aggccgaggc
     3841 ggaactgcgc caggcgctga aggccggggc ctgggacgcc aacagcgggg cccagccctg
     3901 gctgaaccgg cgccggctcg gccgcctgaa gatattggcc acggtggaac tggccttcga
     3961 tttccaggtc aagcaaccgc cgaaaccgaa gacggaaatt ttcctggtca tggaaacccc
     4021 ggaacgattt cagatccgct tcaacggccg gccgctgcgg cgcccggccc atcccggctg
     4081 gtgggttgac ccggccttcc gcaaacttcc ggccgccggc cggctgcggc cgggccggaa
     4141 cgaaatcatc ctgcgcaccg gcttccggtc cgacaccgaa ctccagaacc tttttataac
     4201 cggggacttc gccgtcgccg gccccggccg gcgccgtttc cggctgaccg ccgccgaacc
     4261 ggagcgcctc cgctccggcg cctggaccgc tcagggctat cccttcttcg ccggccggct
     4321 ggcggcggcg caggagttcg agctgaagga gaatctcggc cgggtctggc tggaactgga
     4381 aaacccggcc gcggccgttt accaggtgcg gatcaacggc cggagcgccg gcacgctgct
     4441 ctggccgccc taccggctgg agataaccgg gctggcccgg ccgggccgca accggctgga
     4501 actggaggcg gtcaactccc tgcgcaacct gctcgggccg caccattacc ggataccggc
     4561 cggccgccgc ttcgaccagg agcgcgaact ggtcggcccc ggcacctttc gaaacgaaaa
     4621 gaagtggacc gaaacctacc agctggtctc cgatggactg ccgctggcca gcctgctatt
     4681 cccaaggagg gaaacgtgag gaaattccgg attacgatcg gtctggccgc gctgatcgcc
     4741 gccgcgctgc cggggattgt ccgggcccag gtcgccccgg gcgtcaactg gctcaagaac
     4801 cccggctttg aagaggtcgg caccggcggc agcctacccg gcggctacac cgtcggcggc
     4861 aatccgaagg tggagtgggt caaggaggcc cacagcggga actacgcgct cgccctggcc
     4921 ccgatgcagg ccagcggcaa ctggttccgc ttcccgatca acgtcccggg cggcccgccc
     4981 cgcaagttcg tcttctcctt ctggacccgg accgaattcg acccggcgac cggcgactca
     5041 ccctccttct atctccacat gtccacgccc ggcttcgtcg gggccggcgg cagctttccg
     5101 cgcacccgtt tccgctacct cggcgagtgg acccacatcg gcggcctggt cacctccgac
     5161 atcaacacca tcggctggtt ccgcttcatc ctttatacca ccaagcccgg cctgcgcatc
     5221 ttcctggacg acctcttctt cgccgaagtg accggcctgc cggaggccga gatccagaaa
     5281 ctcctggccg ggaccaccct gaccccgaag atggagctgg ccagcgccgt caaggaggag
     5341 accgactacc agcccggcaa caagtttgcc aactcctcct tcgagctgga gaccggctgc
     5401 ggctggggga tcttcggcgg cgggaagctg gtcccggtcg ccggcggcct ggagggcggc
     5461 cgggccatgc cggccgagcg tctctggtac cagcggctga aactgcgccc ctaccgccgc
     5521 cacaccttct ccggctactt caaggcggcc gccggcagca gtgccaacct ccgggtggaa
     5581 tttctcaacg gctacagcga tggataccgg accaagcagg cggccatcag ccaggacttc
     5641 aaggtcaccg gcgactggca gcgttacaat ttctccttcg tgccgccggc caatccgagc
     5701 ggcaacacct ataccctgcg cctgagcggc cgggtcctgg ccgacaacct ggcgctcgag
     5761 gagggcgacc tgcgccccta tcagccggcc cgcaccctgg aacacggaat ctccac
//
LOCUS       DAOQYN010000123         5746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_14287_length_5746_cov_5.037428, whole genome shotgun sequence.
ACCESSION   DAOQYN010000123 DAOQYN010000000
VERSION     DAOQYN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14287_length_5746_cov_5.037428"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1217
                     /locus_tag="PKN80_05840"
     CDS             207..1217
                     /locus_tag="PKN80_05840"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNQ35569.1"
                     /translation="MLHRKAELYNVAEMIDLEAAGDRVLSRLPDALRRSLNPHAVKSA
                     PRSAGCEIRFNLKGEQARVVIEHGSEKSLVEVYRGPFLSTWHPLTPGRPCEISVGREA
                     LAPQLKDLAGRADLSYDPDLVRIILPHLGWTRLLELEGDLAPPRPEQTPARRCLMYGS
                     SITHGYHGIRPSGSYAMLTARRLGVDLINLGFGGGAWMEPQLADYIAGRTDWDFAILE
                     MGINVIREMEAADFEARVDYFIARIAAAHPEKWLFCIDVFSFRDDFDPALAKPELFRQ
                     AVRRTVRRVNSKRVVHLDGRRLLTDFGGLTTDLVHPSPDGMAEMADRLAGAVSRRMEK
                     PV"
     gene            complement(1230..2273)
                     /locus_tag="PKN80_05845"
     CDS             complement(1230..2273)
                     /locus_tag="PKN80_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35570.1"
                     /translation="MSAENALSNIHLEAAGRWGHTEYSLGYHREYLARLTGLASDDPG
                     FHRAAWDRLALDFLWLTNDGLVDWKKTGRTTDLGHAVYAVDGSDRRPPSVSPFHGEAE
                     VWAFDAGAEYGLPDFDAQIRAYERLLEDSRRAFPNQLIPGGYYKTIISGAIEAFGWEP
                     LLLAAAEPDRMEKVLDTFFRRTRFFMEAWARTSARVIIQHDDFTWTAGPFLSPGFCRR
                     VLIPRYAELWKPLHAAGQKVLFCSDGRYQEFIPDLAAAGADGFIFEPVNDFAWLAANF
                     GEGYCLVGSDVDCRDLAAGHWEKAEAGLRRTFRALERCRGAILAVGNHLPPGLPEAML
                     NRYFDFLLPRLAR"
     gene            complement(2320..3210)
                     /locus_tag="PKN80_05850"
     CDS             complement(2320..3210)
                     /locus_tag="PKN80_05850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ35571.1"
                     /translation="MKRSGLIILSGFFWLTLGLTGRLAASEIRIRPERPVGGQGILVE
                     LAASDSEIVRVRHQERLYHAYRASSGGREIFLPLRVGAAGSLPVLVERISADSAVEKT
                     DLALEVSARPAESVKISAGGVRMRRRQPSRAEERDLVLDSLRRCSETRRWKEVFIRPL
                     QGKPGTGFGVTRWRGDYQARHWGVDFIAQKGTPVAAVNSGLVVLAEHGYNIYGNLVVV
                     DHGQGVVSAYFHLDRILKKQGDVVERGETIGTVGSTGWSSGPHLHLALYLQGAPVDPL
                     WFIDFTRTLSGAAPGGAKSP"
     gene            3371..4663
                     /locus_tag="PKN80_05855"
     CDS             3371..4663
                     /locus_tag="PKN80_05855"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ35572.1"
                     /translation="MNEKKRPVLAINGGERSIPEGFIKNWPPIDPVDEEYVLASLRGE
                     NHTFGPNCTAFEKEFAAWNGNQYAITTNSGTAALHMAVYSCDCGSGDEVIVPAYSWSS
                     SATCILHHNAIPVFVDIDYATMNLDPDKIEAAITEKTKAIIVVHLHGLMADMEKITRL
                     ARKYGLKVIEDACQGHGARFQEKKSGHWGDCATFSFNQNKCLCSGEGGMFVTDNREMW
                     EKARTLWSFGETRSPVESRDYHAYALGWMYRNNDLTAAFGRAQLTKLDRYLAVQAENA
                     AVLLEELKGQPGLILPFVPEGHQPNWYNFTLRIDPEAFGCPSPTFRFRDAVLKAVRAE
                     GVPTGIWQSYILPRMTVFQSRNGYGQGCPWICPNARNVIYNPEAYPAAQKHCETHFGL
                     TTPLRAPNGTGVARKVAAAIRKVLENAGELNLEPEPKK"
     gene            4660..5487
                     /locus_tag="PKN80_05860"
     CDS             4660..5487
                     /locus_tag="PKN80_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ35573.1"
                     /translation="MKISMMSYTLARGEWGKNPDIERLCRFTRELGLDGVDWVTLYGR
                     EAGEVRRIMDDHGLKTVCYTFFVDLNFPEAELRRPGLEELKRGVETAVQLGTDKVMLP
                     IRSKPEFSRDESRRHVIEGLKEGVRFAAEAGVRLSVEHFPDPRSPFVVSADVNQALRE
                     IPELGVTFDSGNVLTGGEDPVAGFLKSKDRVIHAHFKDWVRTEGAPGRAGLDGKFYQP
                     ALVGEGIVDHRSVLEAMHQAGYRGYLNLEYEGNLYPPEAAMEKGLRFLNGLLESLPD"
     gene            complement(5542..>5746)
                     /locus_tag="PKN80_05865"
     CDS             complement(5542..>5746)
                     /locus_tag="PKN80_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35574.1"
                     /translation="DGQAMTWEKRLALAGPDTDTLTGANRDQGGQGIHFSPAGLRNHG
                     RLWVEKVAAYLDSLADDGPASTV"
BASE COUNT         1171 a         1842 c         1813 g          920 t
ORIGIN      
        1 taccaggtga aactccggta ccggtacgtg gacgaagcct tcaacggccg gccggaccag
       61 cggacgaccg ggaattatcc gatgagcgac tacttctaca gccggccgtt cctcaaggtg
      121 gtttacgagg accggctgcc cgggctggtg gtcgaagtaa caaataaata aatcgcccgg
      181 caacttaaaa aagaggggga ggaccgatgc tgcatcggaa ggccgaactg tacaacgtgg
      241 ccgaaatgat tgacctcgag gcggccggcg accgggtatt aagccggctg cccgacgcct
      301 tgcgcagaag cctcaacccg cacgcggtga aatcggcccc gcgctcggcc ggctgtgaaa
      361 tccggttcaa tctcaagggc gaacaggcgc gggtggtcat cgaacacggc agcgagaagt
      421 ctctggttga ggtctaccgc ggccccttcc tgtcaacctg gcacccgcta acgccgggcc
      481 gtccgtgcga aataagcgtg ggccgcgagg ccctggcgcc ccagttgaaa gacctcgccg
      541 gacgggccga cctctcctac gacccggacc tggtccggat cattcttcct cacctgggtt
      601 ggacgcggct gctggagctg gaaggcgacc ttgccccgcc gcggccggag cagaccccgg
      661 cccggcgctg cctgatgtac ggctcctcca tcacccacgg ctaccacgga ataaggccga
      721 gcggcagtta tgccatgctg accgcccggc ggctgggcgt ggacctgatc aacctcggct
      781 tcggcggcgg cgcctggatg gagccccagc tggccgatta tatcgccgga cgaaccgact
      841 gggacttcgc catcctggag atgggcatca acgtgatccg ggagatggag gcggcggatt
      901 tcgaggcccg ggtcgattat ttcatcgccc ggatcgccgc cgcccacccg gaaaaatggc
      961 tcttctgcat cgacgtcttc agcttccgcg acgattttga ccccgccctg gccaagccgg
     1021 aactcttccg gcaggcggtg cggcggacgg tgcgcagagt gaattcaaaa cgggtggtcc
     1081 atctggacgg gcggcggctg ctgacggatt tcggcggcct gaccaccgac ctggtccatc
     1141 cttcgcccga cggcatggcg gagatggcgg accggctggc cggggccgtc tccaggcgaa
     1201 tggaaaaacc ggtctgatac cgggccggct caacgcgcca gccgcggaag caggaaatcg
     1261 aaataacggt tgagcatcgc ctccggaagg ccgggcggca ggtggttgcc gaccgccagg
     1321 atggctccgc ggcacctttc aagtgcccgg aaggtacgcc tcaacccggc ctcggccttc
     1381 tcccaatggc cggccgccag gtcccggcaa tccacgtcgc tgcccaccag acaataacct
     1441 tccccgaaat ttgcggccag ccaggcgaaa tcgttgaccg gttcgaagat gaaaccatcg
     1501 gcgccggcgg cggctaggtc gggaatgaac tcctggtaac ggccgtcgga acagaagagc
     1561 accttctggc cggcggcgtg aaggggtttc cagagttcgg cgtaacgcgg aatcagaacc
     1621 cggcggcaga agccggggga gagaaaggga ccggcggtcc aggtgaaatc gtcgtgctgg
     1681 atgatgaccc gggcgctggt ccgggcccag gcctccatga agaaacgggt ccgccggaag
     1741 aaggtgtcaa gtaccttttc catccgatcc ggttcggcgg cggccaggag cagcggctcc
     1801 cagccgaagg cctcgatggc cccggaaata atggttttat aatagccgcc cgggataagc
     1861 tggttgggaa aagcccggcg cgaatcttca agaagccgct cgtaagccct gatctgggca
     1921 tcgaaatcgg gcaggccgta ttcggcgccg gcgtcgaagg cccagacctc cgcctcgcca
     1981 tggaagggtg aaacagaggg cggccggcgg tcgctgccgt caacggcata gacggcgtgg
     2041 cccaaatcgg tggtccggcc ggtctttttc caatcgacca ggccgtcgtt ggtcagccag
     2101 agaaaatcaa gggcaaggcg atcccaggcc gcccgatgaa aaccggggtc gtccgaagcc
     2161 aggccggtca accgggccag gtattcccgg tgataaccca ggctgtactc ggtatgaccc
     2221 cagcgtccgg ccgcctccag gtggatgttg gacaatgcgt tttccgcgct catcctgaag
     2281 cgccccttcg aaacagaccg gccgcaagag gagacggcct cacggcgatt tggcgccgcc
     2341 gggggcggca ccggaaagag tccgggtaaa atcgatgaac cagagcgggt caaccggggc
     2401 gccctggaga tagagagcca ggtggagatg ggggccgctc gaccagccgg tgctgccgac
     2461 cgtgcctatt gtctcgcccc gttccaccac atcaccttgc ttcttcagaa tccgatccag
     2521 gtggaaatag gcgctgacca ccccctgccc gtggtcgacc acgaccaggt tcccgtaaat
     2581 gttgtaaccg tgttcagcca gaaccaccag cccggagttg accgcggcaa ccggggttcc
     2641 cttctgggcg ataaaatcca cgccccaatg gcgggcctgg taatcgcccc gccaccttgt
     2701 cacgccgaaa ccggtccccg gttttccctg gagcggccgg atgaaaactt ctttccatcg
     2761 gcgcgtctcc gaacaccggc gcagggaatc gaggaccagg tcgcgctcct cggcccggga
     2821 gggctggcgg cggcgcatcc ggaccccgcc ggcgcttatt ttcaccgatt cagccggccg
     2881 ggccgacact tcaagagcca ggtcggtctt ctccacggcg ctgtcggccg aaattctctc
     2941 taccaggacc gggagactcc cggcggcgcc aaccctcagc ggcagaaaaa tttcacggcc
     3001 gccggaggaa gcccggtagg catgatataa ccgctcctgg tggcgtaccc ggacaatctc
     3061 gctgtccgaa gcggccagct cgaccaatat cccctggccg ccgaccggcc gctccgggcg
     3121 tatccggatt tcggaggcgg cgagccgacc ggtcaagccg agcgtcagcc agaaaaaacc
     3181 ggaaaggatt atcagccctg atcttttcat gtcgtctcga tcctctcgtt cggtatcaat
     3241 ccccccgggg atttttatct tatctcaaaa tatcctccaa aggcaaaaat ccgccggaag
     3301 agcgttgaca attaaaccgc aaagttgtaa actaaatctt ttaaaacgga accgaccaga
     3361 aggaggcagg gtgaacgaga agaaaagacc cgtcctggcc atcaacggcg gggaacgaag
     3421 catcccggaa ggattcatca agaactggcc gcccatcgat ccggtggacg aggagtacgt
     3481 gctggcctcg ctgcggggtg aaaatcacac cttcggcccc aactgcaccg cctttgaaaa
     3541 ggaattcgcc gcctggaacg gcaaccagta cgccatcacc accaacagcg gtaccgccgc
     3601 cctgcacatg gcggtctaca gctgcgattg cggctccggc gacgaggtga tcgtaccggc
     3661 ctactcctgg tcctccagcg ccacctgcat actgcaccac aacgccatcc cggtcttcgt
     3721 ggacattgat tatgcgacca tgaacctgga tcccgacaaa atcgaagcgg ccatcaccga
     3781 aaagacgaaa gcgatcatcg tggtccacct gcacggtctg atggccgaca tggaaaaaat
     3841 aacgcggctg gccaggaagt acggactgaa ggtgatcgag gacgcctgcc agggccacgg
     3901 cgcccgtttc caggagaaga aatccggaca ctggggcgac tgcgccacct tcagcttcaa
     3961 tcagaacaag tgcctctgct ccggcgaagg cggcatgttc gtcaccgaca accgggagat
     4021 gtgggagaag gcgcgcaccc tctggtcctt cggcgaaacc cggagcccgg tcgaaagccg
     4081 cgattaccac gcctacgccc tgggctggat gtaccgcaac aacgacctga cggccgcctt
     4141 cggacgggcc cagctgacca agctggatcg ctacctggcc gtccaggcgg agaacgccgc
     4201 ggtactcctg gaggaactga aggggcagcc gggactgatc ctgcccttcg ttccggaagg
     4261 gcaccagcct aactggtaca atttcacgtt gcggatcgac ccggaggcct tcggctgccc
     4321 gagcccgacc ttccgtttcc gggacgcggt actgaaggcc gtacgggccg agggtgtccc
     4381 aaccggcatc tggcagagct acatcctgcc ccggatgacc gtctttcagt cccgaaacgg
     4441 ttacgggcag ggctgcccct ggatctgtcc caacgcccgg aacgtcattt acaacccgga
     4501 agcctacccg gccgcccaga agcattgcga gacccacttc ggcctgacca cacccctgcg
     4561 cgccccgaac ggaaccgggg tggcccgaaa agtggccgcg gccatcagga aggtcctgga
     4621 gaacgccggc gagttgaacc tggaaccgga gccgaaaaaa tgaaaatctc gatgatgtcc
     4681 tacacgctgg cccggggcga atggggaaag aacccggata tcgagcgcct ctgccgattc
     4741 acccgggaac tcggcctgga cggggtggac tgggttacgc tttacggccg cgaagccggc
     4801 gaggtccggc gcatcatgga tgaccacggt ctgaagaccg tctgctacac cttcttcgtc
     4861 gatctcaact tcccggaagc cgaactccgg cggcccggcc tggaggaatt gaagcggggc
     4921 gtcgaaacgg ccgtccaact ggggaccgac aaggtgatgc tgcccatccg gagcaaaccg
     4981 gagttttcac gcgacgaaag ccggcgccac gtcatcgaag ggctgaagga gggggtccgt
     5041 ttcgcggccg aggccggggt gcgcctgagc gtcgaacact tcccggatcc gcgcagtccc
     5101 ttcgtggtct cggcggatgt caaccaggcg ctgcgggaga tcccggaact gggggtcacc
     5161 tttgacagcg gcaacgtctt gaccggcggt gaggatccgg tggccggatt cctgaaatca
     5221 aaggaccggg tcatccacgc ccacttcaag gactgggtac ggaccgaggg cgcgccggga
     5281 cgggccggcc tggacggaaa attctaccag ccggccctgg tgggcgaggg catcgtcgat
     5341 catcggagcg tgctggaggc gatgcaccag gccgggtacc ggggatacct gaacctggaa
     5401 tacgagggaa acctctaccc gcccgaagcg gccatggaaa aggggctgcg gtttctcaac
     5461 ggactgctcg agtcactgcc ggattaaggc ctttttgaaa ccggccggag ggggccggaa
     5521 cggcccccct gccccgccca ttcaaacggt tgaagccggc ccgtcgtcgg caagcgaatc
     5581 caggtaagcg gccacctttt ccacccagag gcggccgtgg ttgcgcaacc cggccggact
     5641 gaaatggatc ccctggccgc cctggtcccg gttggcgccg gtcagggtgt cggtatccgg
     5701 cccggccagg gccagccgtt tctcccaggt catcgcctgc ccatcc
//
LOCUS       DAOQYN010000124         5714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_14366_length_5714_cov_3.994522, whole genome shotgun sequence.
ACCESSION   DAOQYN010000124 DAOQYN010000000
VERSION     DAOQYN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14366_length_5714_cov_3.994522"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /gene="aroC"
                     /locus_tag="PKN80_05870"
     CDS             complement(<1..903)
                     /gene="aroC"
                     /locus_tag="PKN80_05870"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNQ35575.1"
                     /translation="MSRLRFLTAGESHGPALAVIIEGLPAGLDLTGADINRDLARRQI
                     GYGRGGRMAIEQDRAEILSGIRKGLTLGSPIALVIRNRDWENWRENMAAEPGPPAEPV
                     TCPRPGHADLAGLVKYGFSDIRNVLERASARETAARVAAGAAARKLLAALSINIYSRV
                     VRIGSVTDRSRFNPPDDYERIEASRLRCRSVSAENRMAELIDRVKARGESLGGIFEIT
                     VTGLPVGLGSYVHWDRRLDGRLAQALASIPAIKGVEIGAGFQAAARSGSRVQDEIFHD
                     PGRGYFRKSNRAGGLEGGLTNGEPL"
     gene            complement(938..2149)
                     /gene="glgC"
                     /locus_tag="PKN80_05875"
     CDS             complement(938..2149)
                     /gene="glgC"
                     /locus_tag="PKN80_05875"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNQ35576.1"
                     /translation="MNELIVFLLAGGKGERLYPLTKNRAKPAVPFGGNYRIVDFPLSN
                     CINSNLRKIFVLTQYKTISLDRHIRMGWSILSPELGEHVTIIPAQQRISEDWYRGTAD
                     AVFQNIYTLDRERSRLVLILAGDHIYKMDYRPMIEFHNQSGAEITVGVMDIPIRQASR
                     FGVLEINGDDRIVAFKEKPADPQPIPGFPDRALISMGIYLFKTESLVREVIRDAKVEE
                     STHDFGRDILPRVIQEKKSVFAYPFQDWRKNEKRYWRDVGTIQAYYEASMDLVSVSPE
                     LDLYDPTWPIRSYLPPLPPAKTVFQENGRTGQILDSLICNGSIVSGSTVIHSIVAPRV
                     FLHSYSRIEDSVILEGVEIGRHVRIRRAIIDKFVRVPDGVEIGYNLEADRHRFTVNDE
                     GIVVIPERASI"
     gene            complement(2146..2508)
                     /locus_tag="PKN80_05880"
     CDS             complement(2146..2508)
                     /locus_tag="PKN80_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ35577.1"
                     /translation="MKTILVVDDDNAQRTILQKELEPEGYRVRLAESGSEALDLIRKE
                     MIDLVVLDIRMPEMDGLELLGKMLAFKPGLPVVFYTAFDSYQDSFFSWAADAYVLKDP
                     DVGNLKAKLKELLGGDAA"
     gene            complement(2505..3917)
                     /locus_tag="PKN80_05885"
     CDS             complement(2505..3917)
                     /locus_tag="PKN80_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ35578.1"
                     /translation="MVVPIRKEVFWTDDEVGLLYRLDEALHSTRDIEHLFFIILTAAT
                     AGSAFSFPRAALLLLDESRSRLRGVLGIGPNSQAEASRIWSELDGAKPGLHNLLANYE
                     HFDRNGEFNRAVLALDFNLSDCPVCGQVVRSAEVFMLTRPLETGALHPELAALFSGPQ
                     VVLTPIPDQTAPLGMLAADNAFSGRPVSERQVQLLAFFSERAGMILEMVRINQELNQK
                     LKELNQAYHELYETKNNLTRSAHLASIGRSISYVSHEIREPLAVIGGLARIISRASSD
                     PESREQGLQIYQKCLELSRFVDNSLVYARLGRSEITACRVNDLITEVSEDLFRKLAEE
                     TGRKTAPVTISLKPGLPEIMADPEQLRHVFLNLIENGLFSIRDLSEGRVRVTTDFTRE
                     SIIVAVDDNGHGIAAGDLDKIFEPFYTTRLTGSGLGLAFAREAVENNGGTIRVESRGP
                     EEGARFVVTLPRKRGRGDQP"
     gene            complement(3921..4610)
                     /locus_tag="PKN80_05890"
     CDS             complement(3921..4610)
                     /locus_tag="PKN80_05890"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNQ35579.1"
                     /translation="MIRFLGKKKVSKRIFWALGIIIIPSFMIWGVGMEVSGSRKNTAL
                     RINRTRISVDQYNKLLAAALQQYSNYYGGYPAQDSPQMKSIKDQVVEQSIQETLIDQE
                     AARRRLRVSDREVLAMVRSNPSFKDEKGNFNEQLFRDAVNRISDEEMIRIEKEIRRSI
                     LISKLQSSVVNEEALKVTPEELASFRARNPGVQLPDEQLQNVLIQQKSAEAFESWYKG
                     VRARARINRQI"
     gene            complement(4576..5307)
                     /gene="rsmI"
                     /locus_tag="PKN80_05895"
     CDS             complement(4576..5307)
                     /gene="rsmI"
                     /locus_tag="PKN80_05895"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNQ35580.1"
                     /translation="MNVPGTLYVVATPIGNLDDITIRALKILQTADLVAAEDTRETRK
                     LLSHFQCPAANRLISFYRDNEKGRIERIMGALNRGENVAIVSSRGTPGISDPAYLLVR
                     AAHQAGLPVVPIPGASSLAAALSASGVPTDRFCFVGFLPRRPSKRRRIIEELKTRDET
                     AVIYESPHRFLKTMSELHSALGDRELTICRELTKKFEEIRLGGFADLIEHFSSRPVRG
                     EFIIILRASSSKEKHDQIPGQEEGI"
BASE COUNT         1020 a         1658 c         1823 g         1213 t
ORIGIN      
        1 aagcggttca ccgttggtga ggccgccctc cagtccgccg gcccggttgc tcttcctgaa
       61 gtaaccgcgg ccagggtcat ggaagatttc gtcctggacc cgggagccgc ttcgggcggc
      121 cgcctggaac ccggcgccga tttccacgcc cttgatggcc ggtattgaag ccagggcctg
      181 ggccagccgc ccatccagcc ggcggtccca gtggacgtaa ctgcccaggc cgaccggcag
      241 tccggttacc gtgatttcaa agatgccgcc cagggattct ccccgggcct ttacccggtc
      301 gatcaattcg gccatccggt tctcggcgct taccgatcgg cagcgcagcc ggctggcctc
      361 gatccgttcg taatcgtcgg gaggattgaa acggctccgg tcggtgacgc tcccgattcg
      421 aaccacccga ctgtagatgt tgattgataa agcggccagg agttttcggg ccgcggcccc
      481 ggcggccacc cgggcggcgg tctcgcgggc gctggccctt tccagcacgt tccggatgtc
      541 gctgaaaccg tatttgacca gaccggccag gtcggcatgt cccggccggg ggcaggtgac
      601 cggctcggcc ggcggacccg gctccgcggc catgttttcc cgccagtttt cccaatcccg
      661 gttccggatg accagggcaa tcggactgcc cagggtcagg cccttgcgaa tcccggacag
      721 gatttcggcc cggtcctgtt cgatggccat gcggccgccc cggccgtaac cgatctggcg
      781 gcgggccagg tcgcggttga tgtcggcccc ggtcaggtcc agcccggccg ggaggccttc
      841 gatgattacc gccagggcgg gaccatggct ttcgccggcg gtcaggaatc gtaatcggct
      901 catatggata taattataac cgtttttgag acggcggtca tattgaggcg cgttccggga
      961 tgaccacgat accctcgtcg ttgaccgtga accgatggcg gtccgcttcc aggttgtagc
     1021 caatctcgac cccgtccggg acccggacga acttgtcgat gatggcccgg cggatgcgga
     1081 catggcggcc gatctccacc ccttccagga ttacggaatc ctcgatccgg ctgtaactgt
     1141 gcaggaagac ccggggggcg acgatggagt ggatgaccgt ggaaccgctg acgatggagc
     1201 cgttgcagat gagcgaatcg agaatctgcc cggtgcgtcc gttttcctga aagacggtct
     1261 tggccggagg caggggcggc aggtaggaac ggatcggcca ggtcggatcg tagaggtcaa
     1321 gttcgggact gaccgagacc aggtccatgc tggcctcgta ataagcctgg atggttccca
     1381 cgtcccgcca gtagcgtttt tcgtttttgc gccaatcctg gaacgggtag gcgaagaccg
     1441 atttcttctc ctggatgacc cgcggcagga tgtcgcggcc gaaatcgtgg gtgctctctt
     1501 cgaccttggc gtcccggatg acttcccgaa ccagcgactc ggtcttgaag agatagatgc
     1561 ccatcgaaat gagcgctcgg tccgggaagc ccgggatggg ctgcggatcg gccggttttt
     1621 ccttgaaggc cacgatgcgg tcgtcgccgt ttatctccag caccccgaag cggctggcct
     1681 gccggatcgg tatgtccatc acccccacgg tgatctcggc cccgctttga ttgtggaatt
     1741 cgatcatcgg ccggtagtcc atcttgtaaa tgtggtcgcc ggccaggatc aggaccagcc
     1801 ggcttctttc ccggtcaagc gtgtagatgt tctggaaaac ggcatcggcg gtcccccggt
     1861 accaatcctc gctgatgcgc tgctgggccg ggatgatcgt tacgtgttcc cccagttccg
     1921 ggctgagaat gctccagccc atccggatgt gccggtcaag ggagatggtt ttgtactggg
     1981 tcagcacgaa tattttgcgc aggttgctgt tgatgcagtt gctgagcggg aaatcaacga
     2041 tgcggtagtt gcctccgaaa ggcaccgccg gcttggcccg gttcttggtc agcgggtaga
     2101 ggcgctcccc cttgccgccg gccagcagga agacaatcag ttcgttcatg cggcgtctcc
     2161 gcccaggagt tccttcagct tggccttcag gtttccgacg tccgggtcct ttaaaacgta
     2221 ggcgtcggcc gcccaggaga agaaactgtc ttggtagctg tcgaaggcgg tgtagaagac
     2281 caccggcagg cccggtttga aggccagcat cttccccagc agttccaggc cgtccatctc
     2341 gggcatccgg atgtccagca cgaccaggtc gatcatttcc ttcctgatca ggtccagggc
     2401 ttccgagccg ctttcagcca ggcggacccg atatccttcc ggttccagct ctttctgcag
     2461 gatcgtccgc tgagcgttgt cgtcgtcaac caccagaatg gttttcatgg ttggtccccc
     2521 cttccccttt ttcgcggcag cgttacgacg aaccgggcac cctcttccgg tccccggctt
     2581 tccaccctga tggtgccgcc gttattttcc accgcctccc gggcgaaggc caggccgagg
     2641 ccgctgccgg taaggcgcgt ggtgtaaaac ggttcgaata ttttgtccag gtcgccggcc
     2701 gcgattccat gtccgttgtc atcgacggcg acgataatcg actcccgggt gaagtcggtc
     2761 gtcacccgga cccggccttc ggaaagatcc cggattgaaa atagtccgtt ttcaatcagg
     2821 ttgagaaaga catggcgcag ctgttccgga tcggccatta tctccgggag gcccggtttg
     2881 agggagatgg ttaccggcgc cgttttccgt ccggtctcct cggccagttt cctgaagagg
     2941 tcttcgctca cctcggtgat gagatcgttg acgcggcagg cggtaatctc ggaacgtccg
     3001 aggcgcgcgt ataccaggct gttgtcgacg aaacgggaaa gctcaaggca tttttgataa
     3061 atctggaggc cctgctcccg gctttccgga tcggaagagg cgcgggaaat gatccgggcc
     3121 aggccgccga ttaccgcgag tggctcccgg atctcgtggc tcacgtaaga gatggagcgt
     3181 ccgatcgagg ccaggtgggc gctgcgggtc aggttgtttt tggtttcgta aagctcgtgg
     3241 taggcctggt tgagttcctt cagtttctga ttgagttcct ggttgatccg gaccatctcc
     3301 aggatcatgc cggctcgttc ggagaagaag gccagcagct ggacctggcg ttcgctgacc
     3361 ggccggccgg agaaggcatt atccgccgcc agcatgccca gcggagccgt ctggtcggga
     3421 atgggggtca gcaccacctg gggcccggag aagagggccg ccagttccgg gtgcagggcc
     3481 ccggtttcca gcggccgggt cagcatgaat acctcggcgg atcggactac ctgtccgcag
     3541 accgggcagt cggagaggtt gaagtcgagt gcgagcaccg cccggttgaa ctccccgttt
     3601 cggtcgaagt gttcgtagtt ggcaagaaga ttgtgaaggc cgggcttggc gccgtccagc
     3661 tcgctccaga ttcggctggc ttcggcctgc gagttggggc cgatgcccag gacaccccgg
     3721 agccggctcc gggattcgtc caggagcagg agggcggccc gggggaaact gaaggcactg
     3781 ccggccgtgg ccgcggtaag aatgatgaag aaaaggtgtt cgatatcccg ggtggagtga
     3841 agggcctcgt caagccggta gagaagccct acctcgtcgt cggtccagaa gacttctttt
     3901 ctgatcggta cgaccatata tcagatctgg cggtttatgc gggcccgggc tcgcactccc
     3961 ttgtaccagg attcgaaagc ctccgcggat ttttgctgga tgaggacgtt ttgaagctgt
     4021 tcgtcgggaa gctggacacc cggattcctg gcccggaatg aggccagttc ttcgggcgtg
     4081 accttgagag cctcctcgtt aacgacgctc gactggagtt tgctgatgag aatggaacgg
     4141 cggatctcct tctcgatgcg gatcatctct tcgtcggaga tccggtttac cgcgtcgcgg
     4201 aagagctgct cattgaaatt acccttttca tccttgaaag aggggttgga acggaccatg
     4261 gccagcactt cccggtccga aacccgcagt cttcgccgcg ccgcctcctg gtcgatcagg
     4321 gtctcctgga tactctgctc gaccacctgg tccttgatgc tcttcatctg gggggaatcc
     4381 tgggccgggt agccgccgta gtagttgctg tactgctgaa gagcggcggc cagcagcttg
     4441 ttgtactggt cgacgctgat ccgggtccgg ttgatgcgca gcgcggtatt tttgcggctg
     4501 ccgctcacct ccatgcccac cccccagatc atgaaggagg ggatgatgat gatgcccaaa
     4561 gcccagaata tacgcttaga taccttcttc ttgcccagga atctgatcat gtttctcctt
     4621 tgaagatgag gctctcagaa taatgatgaa ttctccccgg accggccggc ttgagaagtg
     4681 ttcgatcagg tcggcaaagc cgccgagccg gatttcctcg aatttcttgg tcaattcgcg
     4741 gcagatggtt aattcccggt ctccgagcgc cgaatggagt tcggacatgg ttttaagaaa
     4801 acgatgcggc gattcgtaga tcaccgcggt ttcatcgcgc gtcttcaact cctcgataat
     4861 ccggcgccgc ttggacggcc ggcgcggcag aaagccgacg aaacagaagc ggtcggtcgg
     4921 tacgccgctg gccgaaagcg ccgccgccag ggaagaggcg ccgggtatcg ggaccaccgg
     4981 gaggccggcc tggtgggccg cccggaccag caggtaggcc gggtcggaga tcccgggcgt
     5041 gccccggctg gagactatgg caacattctc cccccggttc agggctccca tgatccgttc
     5101 gatccgccct ttttcgttgt ctcggtaaaa gctgatgagg cggttggcgg ccgggcactg
     5161 aaaatgactc agcagtttcc tggtttcccg ggtatcctcg gcggccacca ggtcggccgt
     5221 ttgaaggatc tttaaggccc ggatggtgat gtcgtcaagg ttcccgattg gagtggccac
     5281 gacgtaaaga gtgccaggga cgttcatgtc tttattatac cccaaaaaga cggcgccgaa
     5341 aaaggtgcgc ggcggttgtc gaagagggcc gagcctgggg aaaagaccgt gaacaaggat
     5401 aagaccggcc ggcgtaatga ttgggatgcc tggatttcaa ctgccagggc cgtgtttttt
     5461 ggcctttttt tattgacggg ggccgacgga aaggccggtt atctcaagat ttcagccccg
     5521 gccggccggg tgtgataaaa tatgaagcgt gtttttctac gcgatccgga aaggccgggt
     5581 ttggaatcaa ctgccggagg acaaatgcgc gaggatattc ttaaatccat aattaccgaa
     5641 aacgacgaga agatagtgct ggtgattgcc gacggtctgg gcggcttacc ggattcggcc
     5701 accggccgca ccga
//
LOCUS       DAOQYN010000125         5692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_14447_length_5692_cov_5.654604, whole genome shotgun sequence.
ACCESSION   DAOQYN010000125 DAOQYN010000000
VERSION     DAOQYN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14447_length_5692_cov_5.654604"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1602
                     /locus_tag="PKN80_05900"
     CDS             148..1602
                     /locus_tag="PKN80_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35581.1"
                     /translation="MTDIMLKFDVEDIYRPESEGGDDRIGWLAELLSEHGLRAQFLLI
                     GDKYRRLIRNRRSDIARALEAHGVGTHTNSQFHPLLPERLESAGWERGVSLVLADERK
                     AVEACRRFTGRDPSALSRHANLTTGAFHFEAARRLGLPYFYADIPGLSATASLCFCNE
                     ALLTAFGGSSLTYASVHDAVYDDEAHFAPRLEQVREFIDRAAADKIPFINIFLGHPAT
                     MRSHSGHMITYVHGGGRDRDPKRHLFGEDAPAIFGPEHEPVLKERFRQTCRMLAERKD
                     VNYLTADQLAGRYNKKSDYIWDRNFDRYCRLVLAENDIPVFGEPYSPAELAANLARLL
                     LVRKRTGRFPQRLEPVRGVLGPLEEPTMRPDPRFIDGKLFWRKVAELDRYIKERGRLP
                     GNLEFGGGDRVGMGSFLEGMCKAYFGLRDAGAVGRVALSLTFPKYPSRPAAGMDEFFC
                     RFHQDPIYRPDLSVENLARFNRLATWTLRLAYRN"
     gene            complement(1689..2738)
                     /locus_tag="PKN80_05905"
     CDS             complement(1689..2738)
                     /locus_tag="PKN80_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35582.1"
                     /translation="MAKQYRVGVIGTGGIARVHLGGWGRLTEARVVAAADINGARLKD
                     FAAEFGIPKTYEDYGEMLKSEELDIVDICAPNSVHCPAALAAFKQGRHVYCEKPMALN
                     AGQVRRMIAASEAAGRKLMAGQQQRFTTQARLVKEKIERGELGEIYYVRAQALRRRYL
                     PARPGFIKKSLSGGGPLVDIGVHILDLTLWLMGFPAVASVSGVAQKRLAGREDIRGEW
                     GEWDRKAYDVEDFAVGQVRFKNGGVLTLECSFLLNMKEREIFGASLFGTEAGVTFPPV
                     EMYREEKGEHLSIQQIPFPDRSGPPHYQAIAAFLEAVKKDRPVPVRPAESLAVMEILD
                     AIYQSSKTGREVRFK"
     gene            complement(2761..4143)
                     /gene="mnmE"
                     /locus_tag="PKN80_05910"
     CDS             complement(2761..4143)
                     /gene="mnmE"
                     /locus_tag="PKN80_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNQ35583.1"
                     /translation="MSNETICAISSPPGLGGIGMIRVSGPEALRLVEQVFRDKRGRPV
                     RLSTLPSHTVRYGFVVSGGRCRDEVLVTVLKGPRTYTREDMVEIGCHGGSVPLRSVLG
                     ALLDAGGRLAGPGEFTRRAFLAGRIDLAQAEAVLAAVNSRTEAGLEAALAQIKGAFSG
                     KLSGLERDLLDLLAGIEAGIEFPDELENPAEPRGRWQAELRRLAERAGRLRRASAEGR
                     IMAEGIRVVIAGRPNVGKSSLLNALLEQERAIVTDVPGTTRDEISELITLAGLPVRLT
                     DTAGIRPPRDRIEKAGVRRSRARVKSADLLLLVLDRSRPLEAADRRLVAGTKVGKTLL
                     VLNKIDRPDRLGALPGRAAGLERVEVSARTGAGLEVLKERVAGLIREGRVSPDSETPV
                     FLGVYQERALRHACEALSEAAAALTDGIPLDFVLASVRRAARHLSEISGRRVDKEVLD
                     QIFSRFCIGK"
     gene            4407..4628
                     /locus_tag="PKN80_05915"
     CDS             4407..4628
                     /locus_tag="PKN80_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35584.1"
                     /translation="MAEQKKSSTAVIVILIVIIVIAIVFAVAKAKKSAAPVTTEPTME
                     GTVPPVAPEGTAPAAPEAPAAPAAPAETK"
     gene            4748..>5692
                     /locus_tag="PKN80_05920"
     rRNA            4748..>5692
                     /locus_tag="PKN80_05920"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1077 a         1806 c         1785 g         1024 t
ORIGIN      
        1 cattccctcg tttcgcttgt tttgcacccg gactccgaga agttctctca tcgaaaacca
       61 gtgttttgaa agagaaagaa aaaaacaact atagggcagg gcggatgatt cagcaaccgt
      121 tctcttcggc tggaaaaagg aaaaaagatg accgatatca tgcttaagtt cgacgttgag
      181 gatatttacc ggccggagtc cgagggtggc gacgaccgga tcggctggct ggcggagctg
      241 ctgagcgagc acgggctgcg ggcgcagttc ctgctgatcg gcgacaagta ccggcggctg
      301 atccgcaacc ggcgctccga catcgcccgg gccctggagg cgcacggggt gggcacccac
      361 accaacagcc agttccaccc gctgttgccc gagcggctgg agtcggccgg ctgggagcgg
      421 ggcgtctcgc tggtgctggc cgacgagcgg aaggcggttg aggcctgccg ccggttcacc
      481 ggccgggatc cctcggccct ctcgcgccac gccaacctca ccaccggcgc cttccatttc
      541 gaggccgccc gccggctggg cctgccctac ttttacgccg acatcccggg cctgtcggcc
      601 accgcctcgc tctgcttctg caacgaagcc ctcctgaccg ccttcggcgg ttcgagcctt
      661 acctacgcct cggtccacga cgccgtttac gacgacgagg cgcactttgc gccgcggctg
      721 gagcaggtgc gggagttcat cgaccgggcg gcggccgata agatcccctt catcaatatc
      781 ttcctgggcc acccggccac gatgcgcagc cacagcggcc acatgatcac ctacgtgcac
      841 gggggcggcc gggaccggga tccgaagcgc cacctcttcg gggaggacgc tccggcgatc
      901 ttcgggccgg agcacgagcc ggtgctcaag gagcgcttcc ggcagacctg ccggatgctg
      961 gccgaacgga aggacgtcaa ctacctgacc gccgaccagc tggccggccg ctacaacaag
     1021 aagtccgact acatctggga ccggaacttc gaccgctact gtcggctggt gctggccgag
     1081 aacgacatcc cggtcttcgg cgagccctac agcccggccg agctggcggc caacctggcg
     1141 cggctgctgc tggtgcggaa acggaccggc cgcttcccgc agcggctgga gccggtgcgg
     1201 ggggtattgg gcccgctgga ggagccgacc atgcggccgg atccgcgctt catcgatggg
     1261 aagctcttct ggaggaaggt ggccgagctg gaccggtaca tcaaggaacg gggacggctg
     1321 ccggggaacc tggagttcgg gggcggggac cgggtcggga tgggctcctt cctggagggg
     1381 atgtgcaagg cctacttcgg cctgcgggac gccggcgcgg tcggccgggt ggcgctctcg
     1441 ctgacctttc ccaagtaccc gtcccggccg gcggccggga tggacgagtt cttctgccgc
     1501 ttccaccagg acccgatcta ccggccggac ctctcggtgg aaaacctggc ccgcttcaac
     1561 cgcctggcca cctggaccct ccgccttgcc tatcgcaact aacggaaaag gggcaccggt
     1621 cttccggtgc ccctttcagc ttcggtggac aacctttcct gacaattccg tctccggccg
     1681 tcaccggttt acttgaatcg cacctcccgg ccggtcttcg aggattggta gatggcatcc
     1741 agtatttcca taaccgccag cgattccgcg ggccggaccg gtaccggccg atccttcttc
     1801 accgcctcca ggaaggcggc gatggcctgg tagtgtggcg ggccgctccg gtccgggaag
     1861 gggatctgct gaatggaaag atgctctccc ttctcctcgc ggtacatctc cacgggcggg
     1921 aaggtgacgc cggcttcggt cccgaagagg ctggccccga agatctcacg ctccttcatg
     1981 ttcagcagga agctgcactc cagggtaagc acgccgccgt ttttgaagcg tacctggccg
     2041 acggcgaagt cctccacgtc ataagccttg cggtcccatt caccccactc gccccggata
     2101 tcctcccggc cggccagccg cttctgggcg acgccgctca ccgaggcaac cgccgggaag
     2161 cccatcagcc agagggttaa atccaggatg tggacgccga tatcgaccag cgggccaccc
     2221 ccggagagcg actttttgat gaaaccgggc cgggccggca ggtagcggcg ccggagcgcc
     2281 tgggcccgga catagtagat ctcgcccagt tcgccccgtt cgatcttctc cttgaccagg
     2341 cgggcttggg tggtaaaacg ctgctgctga ccggccatca gctttcgtcc ggccgcttcg
     2401 gaggcggcga tcatccggcg cacctgaccg gcgttgagcg ccatcggctt ttcgcaatag
     2461 acgtgccggc cctgcttgaa ggcggccagg gcggccggac agtggaccga gttgggagcg
     2521 cagatgtcca cgatatccag ctcctcggac ttcagcatct ccccgtaatc ttcgtaggtc
     2581 ttcggaatcc cgaactcggc cgcgaaatcc ttcaggcgcg ccccgttgat gtcggccgcg
     2641 gccaccactc tggcctccgt caaccggccc cagccgccca ggtgcacccg ggcgatcccg
     2701 ccggtaccga tcacacccac ccgatactgc tttgccatca cgcctccttt attttcggaa
     2761 ttacttcccg atgcagaatc ttgagaatat ctggtccagg acctctttgt caacccgacg
     2821 gcccgaaatt tcagacaggt gccgggcggc ccgccggacc gaagccagca cgaaatcaag
     2881 cgggatcccg tcggtcaggg cggcggccgc ctcggaaagc gcttcacagg cgtggcgaag
     2941 ggcccgttcc tgataaacgc ccagaaagac gggagtctcg gaatcggggg aaacccggcc
     3001 ttcccggatc aagccggcca cccgctcttt caggacctcg aggccggccc cggttcgggc
     3061 cgaaacctca acccgttcca gcccggccgc ccggcccggc aaggccccca ggcggtccgg
     3121 ccggtcgatc ttattcagaa ccagcagggt cttcccgacc ttcgtgccgg ccaccaggcg
     3181 ccggtcggcc gcctccagcg gccggctacg gtccaggacc agcaggagca ggtcggccga
     3241 tttgacccgg gcccggctgc gccgaacccc ggccttttcg atccggtcgc gcggcggccg
     3301 gatgccggcg gtatcggtca atcgtaccgg aaggccggcc agggttatca actcggaaat
     3361 ctcgtcccgg gtggttccgg gtacgtcggt cacgatcgcc cgttcctgtt ccagcagcgc
     3421 gttcaacagg ctgcttttcc cgacgttcgg ccggcccgcg ataaccaccc gtattccttc
     3481 cgccatgatc cgtccttcgg cggatgcccg gcgcagccgg ccggcccgct cggccaggcg
     3541 ccgcaattcc gcctgccagc ggccccgggg ttcagccgga ttctccagtt catccgggaa
     3601 ttcgatcccg gcctcgatcc cggccagcag gtcaaggaga tcccgctcca ggccggacag
     3661 cttcccgctg aaggcaccct tgatctgggc cagggccgcc tccaggccgg cctcggtccg
     3721 gctgtttacc gcggccagga ccgcctcggc ctgggccagg tcgatgcggc cggccaggaa
     3781 ggcccggcgg gtgaattcgc ccggaccggc caggcggccg ccggcgtcca gcagggcgcc
     3841 cagcaccgac ctcagcggaa ccgacccgcc gtggcaacca atttcaacca tgtcctcccg
     3901 ggtgtaggtc cgcggcccct tcaggacggt taccagtacc tcgtcccggc aacggccgcc
     3961 gctcaccacg aatccgtacc gcacagtgtg ggagggcagg gtggacaacc gcaccggccg
     4021 gccccgcttg tcgcggaaga cctgttccac caggcgcagg gcttccggcc cggagacccg
     4081 gatcattcca atcccgccca ggcccggcgg gctggaaatg gcgcagatgg tttcgtttga
     4141 catcggctct tgacaaaatc tgcaaatctg gtataatcta tttcaataaa atttcaaggc
     4201 tttcaagccg gctcgatccg aaaacttgag aaaaatactt tgaggttgta taatattata
     4261 taacacatca ggcaggaagc gattcctcac gcaaggttgc agtcaacctt ctgattcttc
     4321 cgaacccaaa ctttcacaac tggtaacgac cggcttgaga ttttcaaaaa ggatttttcc
     4381 ttaaaggaag ggaggtgaat taaagaatgg ccgagcagaa gaagagcagc acggcggtaa
     4441 tcgtgatcct tatcgtgatc atcgttatcg cgatcgtctt tgcggttgcg aaggcgaaga
     4501 aaagcgccgc cccggtgacc accgaaccca cgatggaggg tacggttccc ccggtggctc
     4561 ctgaaggaac cgccccggcg gctcctgagg cgcccgcagc tcccgcggct cccgcggaga
     4621 ccaagtaaga ccgttgattt aacccggttt cgggtgaaaa ttgacaaact atctccggta
     4681 aggtataatt gtaatttgct ggagattgct ctttgaaaat atgggtgtat gccagttttg
     4741 cgtttgtact tctgagggtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaaga
     4801 catgcaagtc gagcgacgaa accgactttt ttgccgcaag gtggaaaagt tggctgaaag
     4861 cggcacacgg gtgagtaata cttggataat ctacctcccg aagggggata actctacgaa
     4921 agtggggcta ataccgcatg acaccttttc tcgcaagggg aagggttaaa ggctgccggg
     4981 taaccggtgg ttttcgggag atgagtccaa gtcctatcag cttgttggcg gggtaacggc
     5041 ccaccaaggc tatgacgggt agctggtctg agaggacggt cagccacact gggattgaga
     5101 tacggcccag actcctacgg gaggcagcag tcgggaatat tgggcaatgc ccgaaagggt
     5161 gacccagcga cgccgcgtgg aggatgaagg ctttcgagtc gtaaactcct gttcggggag
     5221 acgaagtccc gcaagggatt gacggtatcc ccggagtaag tcccggctaa ctccgtgcca
     5281 gcagccgcgg taagacggag ggggcaagcg ttgtccggat tgactgggtg taaagggtgc
     5341 gtaggttgtt tggcgggtcc ggcattaaat cccccggccc aaccggggaa atgtgttgga
     5401 aactaccaag ctggagtctg aaagaggaga gcggaactcc tggtgtaacg gtgaaatgtg
     5461 tggatatcag gaggaacacc ggtggcgaag gcggctctct agttcaagac tgacactgag
     5521 gcacgaaagc cgggggagca aacaggatta gataccctgg tagtcccggc cctaaacgat
     5581 gggtgttagg tatcggtctt tcgggatcgg tgccgaagct aacgcgttaa acaccccacc
     5641 tggggagtac ggtcgcaagg ctgaaactca aaggaattga cgggggcccg ca
//
LOCUS       DAOQYN010000126         5658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_14567_length_5658_cov_3.818133, whole genome shotgun sequence.
ACCESSION   DAOQYN010000126 DAOQYN010000000
VERSION     DAOQYN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14567_length_5658_cov_3.818133"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /gene="rfaE2"
                     /locus_tag="PKN80_05925"
     CDS             <1..498
                     /gene="rfaE2"
                     /locus_tag="PKN80_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNQ35585.1"
                     /translation="RKAEPPRRRTREKVVTLARLMSFLKKHRGRQKVVFTNGCFDIIH
                     DGHRHLLEAARAEGDLLVVGLNSDDSVRRLKGPDRPLNNQGERARMLASLEAVDRVVI
                     FEEDTPMKLIRTIRPDVLVKGGDYRLDQVVGRETVESYGGRVALVSLVPDRSTTRIIE
                     KVRRG"
     gene            516..1556
                     /gene="thiE"
                     /locus_tag="PKN80_05930"
     CDS             516..1556
                     /gene="thiE"
                     /locus_tag="PKN80_05930"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNQ35586.1"
                     /translation="MEKFYRVLDADFNRAREGLRVLEDCARFYLEEAKLTAALRELRH
                     RLSRLTLDSFPRAALLAGRESERDLGRKFQSPGRSGWTELVQANSLRVSEALRSLEEV
                     SKVLAPAATPGYQELRFRFYQLEKGLARFYRPGLPDFPVYAIVESAGGIEADTAFARR
                     LLAAGVRLLQLRLKCGDDRDFLALAVSLAVECRKAGALFLVNDRPDIALLAGAGGVHL
                     GQRDIPPAEARRLCPHLVLGASVNSEVSARRALEAGADYLAVGAIFPTATKDDANPVG
                     LKMLERVKRLAGERPVVAIGGINPGNAGAVYEAGADYLAVISCLRSGRLEKTIAALAA
                     ARDESSKKLLKP"
     gene            1574..2845
                     /gene="thiC"
                     /locus_tag="PKN80_05935"
     CDS             1574..2845
                     /gene="thiC"
                     /locus_tag="PKN80_05935"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNQ35587.1"
                     /translation="MRKNNLLNTREIEALARREELSPAEFRAGLESGRITVVRNRGGR
                     PLAIGAGCYVKVNANIGTSPSRPEVKEELEKLRAVEAAGADAVMDLSTGGDLARVRRE
                     IMAASRIPVGSVPVYGAVVNAVRKYKAVQKMSAEALWEQIEAEAAGGVAFMTVHTGVS
                     ERIVRGLAEGDRLINIVSRGGSLLACWIVANRRENPLLEDFGRLVKIAARYGTALSLG
                     DGLRPGAVADSTDRFQLEELMNLGRQAAEARRAGVPVLIEGPGHIPLHQVEMNVKLAK
                     QVCHGAPLYLLGPLVTDFAPGYDHIVSAIGGALAAMAGADFLCYVTPSEHLGLPGVKE
                     VREGVYTARIAAHAADLARGKPGALQRDLRISTARRDRDWAGQKRWALNPEKVAEAVG
                     TESDFCSMCGEFCSMRFSEKLSPAKRKRKNG"
     gene            2838..4730
                     /gene="ftsH"
                     /locus_tag="PKN80_05940"
     CDS             2838..4730
                     /gene="ftsH"
                     /locus_tag="PKN80_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNQ35588.1"
                     /translation="MDKKKEPKGRPPQAPLPIQGPNFWKGLLPWVVLFIFIFYLAYSS
                     KMIGGHKVEKLTYSQFLEAIDKGEVTGKVTFQEGNLSGKLSNGKEFRSFSNDPDLYKI
                     MKERKVELDVQPEAGRSIWLQLAAGLLPVFLIVGFFWFMFYRRANQGGGDQILSFGRI
                     RPQPLSESRPKTTFKDVAGYEEAKEELQEVIDFLRDPQRFQKLGGRIPKGVLIIGPPG
                     TGKTLFAKAVAGEAEVSFLSISGSEFVEMFVGVGASRVRDLFRQARKMAPSIIFIDEI
                     DAVGRQRFAGLGGGHDEREQTLNQLLVEMDGFNTDEGIILMAATNRPDVLDPALVRPG
                     RFDRQVMLNLPDLREREAILKVHTRNTKLAADIDLSVIAKSTPGMSGADLSNLCNEAA
                     LLASRKNHTQVEMGDLEEALDKVMMGAQRKSMVLAKDEKEVIAYHEAGHALVQLSLPE
                     SQPIHKMTIIPRGRSLGATHILPEKDIHVESEVYFRNSLVSLLGGRAAEKIVYKRVYT
                     GAESDLQVATELARQMVCEWGMSSRMGPVSYHNRSLGEVFLGRDIARPREYSETTARE
                     IDEEVKSLLESSEREALKILQSRKKTLEKLARALLEKETLSGKEIEELVAPEEPRPDE
                     PKLPLA"
     gene            4738..5310
                     /gene="folE"
                     /locus_tag="PKN80_05945"
     CDS             4738..5310
                     /gene="folE"
                     /locus_tag="PKN80_05945"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNQ35589.1"
                     /translation="MIDKKKLEKATRLFLEGIGEDPDRPELKETPRRVAEMAGELYSG
                     LGRNPAELLKVVFSEEYDELVFVRDIPFYSTCEHHLLPFLGKAHIAYIPEGHRITGLS
                     KLARVVETLSRRPQVQERLTTQVADTIMKTLRPRGVMVVIEAEHLCMTMRGVQKPGCQ
                     AVTSVMRGIFLEDARTRNEALNLLHRDRVG"
     gene            5317..>5658
                     /locus_tag="PKN80_05950"
     CDS             5317..>5658
                     /locus_tag="PKN80_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNQ35590.1"
                     /translation="MERQAEFRLKLERLRALAAERGRAGVLINIQPNFAWATAGGENR
                     VGSATETGAADLLAVDGRAYLLASNIEAGRVMAEELAGLGVEPLAYPWHEPAARRQRL
                     AEVTDPDRLLSD"
BASE COUNT          939 a         1845 c         2010 g          864 t
ORIGIN      
        1 cggaaggcgg agccgccccg gcggcggacc cgggagaagg tggtcacgct ggcccggttg
       61 atgtccttcc tgaaaaagca tcgcggccgc cagaaggtgg tcttcaccaa cggctgcttc
      121 gatatcatcc acgacggaca ccgtcacctg ctggaggcgg cccgggccga gggggatctg
      181 ctggtggtcg gcctcaacag cgacgattcg gtgcgccggc tgaagggtcc ggaccgaccg
      241 ctcaataacc agggcgaacg ggcccgcatg ctggccagcc tggaggcggt ggaccgggtg
      301 gttatcttcg aggaagacac cccgatgaag cttatccgca ccatccggcc cgacgtgctg
      361 gtcaagggcg gcgattaccg gctcgaccag gtggtgggcc gcgagacggt ggagagttac
      421 ggcggccggg tggcgctggt ttccctggtg ccggaccgct ccacgacccg gatcatcgaa
      481 aaggtccggc gcggttgagg gccggccgcc cgccgatgga aaaattttac cgcgtcctgg
      541 atgccgattt taaccgggcc cgcgaggggc tgcgggtgct ggaggattgc gcccgcttct
      601 acctggagga ggcgaagctc accgcggcgc tccgggagct gcgccaccga ctttcgcgcc
      661 tgaccctgga ctcctttccg cgggcggccc tgctggccgg ccgggagagc gaacgggacc
      721 tgggccgcaa gtttcagtcg cccgggcgga gcggctggac ggaactggtc caggccaaca
      781 gcctgcgggt ctccgaggcg ctccgctccc tggaagaggt gagcaaggtc ctggcgccgg
      841 ccgcgacgcc cggctaccag gaactccgtt tccgttttta ccagctggag aaggggctgg
      901 cccgctttta ccggcccggg cttccggact ttccggttta cgcgatcgtc gagtcggccg
      961 gtggaatcga ggccgatacc gcttttgccc ggcgcctgct ggccgccggg gtccggctgc
     1021 tccagctgcg cctgaagtgc ggcgacgacc gggacttcct ggccctggcc gtcagcctgg
     1081 ctgtcgaatg ccggaaggcc ggcgccctct tcctggtcaa cgaccggccg gacatcgccc
     1141 tgctggccgg ggccggcggt gtccacctcg gccagcggga cataccgccg gcggaggcgc
     1201 ggcgcctctg tccgcacctg gtgctggggg cctcggtcaa ctcggaggtt tcggcgcggc
     1261 gggccctgga ggccggggcc gattacctgg cggtcggcgc gatctttccg accgccacca
     1321 aggacgacgc gaatccggtc gggctgaaga tgctggagcg ggtgaagcgg ctggccggcg
     1381 agcggccggt ggttgccatc gggggcatca accccggcaa cgccggcgcg gtttacgagg
     1441 ccggggccga ctacctggcg gtcatctcct gcctgcgctc cggccggctt gagaagacta
     1501 tcgccgcgct ggcggcggcc cgggacgagt ccagtaaaaa gttgttgaag ccatagacca
     1561 attcgaggca acgatgcgca agaataatct tttgaatacc cgcgagatag aggcgctggc
     1621 ccgccgggag gagctcagcc cggccgaatt ccgggccggc ctggaatcgg gccggatcac
     1681 ggtggtccgg aaccgcggcg gccgtccgct ggccatcggc gccggctgct acgtgaaggt
     1741 gaacgccaac atcggcacct cgccttcccg gccggaggtc aaggaagaac tcgagaagct
     1801 gcgggcggtc gaggcggccg gggccgacgc ggtgatggac ctttcgaccg gcggcgacct
     1861 ggcccgggtg cggcgggaga tcatggccgc cagccggata ccggtgggca gcgtgccggt
     1921 ttacggggcg gtggtcaacg ccgtccggaa gtacaaggcc gtccagaaga tgagcgccga
     1981 ggccctctgg gagcagatcg aggccgaggc ggccggcggg gtggccttca tgaccgtcca
     2041 caccggggtc agcgagcgca tcgtgcgcgg cctggccgag ggtgaccgct tgatcaacat
     2101 cgtcagccgg ggcggctctc tgctggcctg ctggatcgtg gccaaccggc gcgaaaaccc
     2161 gctcctggag gatttcggcc gcctggtcaa gattgccgcc cgttacggga ccgccctcag
     2221 cctgggcgac ggacttcgtc ccggcgcggt ggccgacagc accgaccgtt ttcagctcga
     2281 ggaattgatg aacctgggcc gccaggccgc cgaggcgcgc cgggccgggg tgccggtcct
     2341 gatcgagggg ccgggccaca tcccgctgca ccaggtggag atgaacgtga agctggccaa
     2401 gcaggtctgc cacggcgccc cgctttacct gctggggccg ctggtgaccg atttcgcgcc
     2461 cggttatgac cacatcgttt cggccatcgg cggcgcgctg gcggccatgg ccggcgccga
     2521 tttcctctgt tacgtgacgc cctcggagca cctggggctg ccgggcgtga aggaggtccg
     2581 ggaaggggtt tacaccgccc ggatcgccgc ccatgccgcc gacctggccc ggggcaagcc
     2641 gggcgccctg cagcgggacc tccggatttc gaccgcccgc cgcgaccgcg actgggccgg
     2701 ccagaagcgc tgggcgctca acccggagaa ggtggccgag gcggtcggga ccgagtcgga
     2761 tttctgttcc atgtgcggtg agttctgctc gatgcgtttc tctgaaaagc tgtcgccagc
     2821 caaacggaag cggaaaaatg gataagaaga aggaacccaa ggggcggccg ccccaggcgc
     2881 ccttgccgat ccaggggccc aatttctgga agggactgct gccctgggtg gtgctcttca
     2941 tcttcatctt ctacctggcc tactcctcga agatgatcgg cggccacaag gtggagaagc
     3001 tcacctacag ccagttcctg gaggccatcg acaagggcga ggtcacgggc aaggtgacct
     3061 tccaggaggg gaacctctcc gggaagctga gcaacggcaa ggagttccgg agcttcagca
     3121 acgaccccga tctttacaag atcatgaagg aacgcaaggt ggagctggat gtgcagccgg
     3181 aggccggccg ttccatctgg ctgcagctgg cggccggtct gctgccggtc ttcctgatcg
     3241 tgggcttctt ctggttcatg ttctaccggc gcgccaacca gggcggcggc gaccagattc
     3301 tctccttcgg ccgcatccgg ccccagccgc tctccgagtc gcggcccaag acgaccttca
     3361 aagacgtggc cgggtacgag gaggccaagg aggagctgca ggaggtgatc gatttcctgc
     3421 gcgatccgca gcggtttcag aagctgggcg gccggattcc caagggcgtc ctgatcatcg
     3481 ggccgccggg caccggcaag accctctttg ccaaggcggt ggccggcgag gccgaggttt
     3541 ccttcctttc catcagtggc tcggagttcg tggagatgtt cgtcggggtc ggcgcctcgc
     3601 gggtccgcga cctcttccgg caggcgcgca agatggcccc ctcgatcatc ttcatcgacg
     3661 agatcgacgc ggtcggccgc cagcgtttcg ccgggctggg cggcggccac gacgagcggg
     3721 agcagaccct gaaccagctg ctggtcgaga tggacggttt caacaccgac gaagggatca
     3781 tcctgatggc cgccaccaac cggcccgacg tgctggaccc ggccttggtg cggcccggcc
     3841 gcttcgaccg gcaggtgatg ctcaacctgc ccgacctgcg cgagcgggag gcgatcctca
     3901 aggtgcacac ccggaacacc aagctggcgg ccgacatcga cctttcggtg atcgccaaga
     3961 gcaccccggg catgtccggg gcggacctgt ctaatctctg caacgaggcg gccctgctgg
     4021 cctcgcgcaa gaaccacacc caggtcgaga tgggtgacct cgaggaggcg ctggacaagg
     4081 tgatgatggg ggcccagcgc aagagcatgg tcctggccaa ggacgagaag gaggtcatcg
     4141 cctaccacga ggccggccac gccctggtcc agttgagcct gccggagtcg cagcccatcc
     4201 acaagatgac catcatcccg cgcggccgtt ccctgggcgc cacccacatc ctgcccgaga
     4261 aggacatcca cgtcgagagc gaggtttact tccggaactc gctggtcagc ctgctgggcg
     4321 gccgggcggc cgagaagatc gtttacaagc gcgtctacac cggcgccgag agcgacctgc
     4381 aggtggccac cgaactggcc cgccagatgg tctgcgaatg gggcatgagt tcccgcatgg
     4441 ggccggtcag ctaccataac cgctcgctgg gcgaggtctt cctggggcgc gatatcgccc
     4501 ggccgcgcga gtacagcgag acgaccgccc gcgagatcga cgaggaggtc aagagcctcc
     4561 tggagagttc cgagcgcgag gcgctgaaga tactccagtc ccgcaagaag acgctggaga
     4621 aactggcccg cgccctgctg gagaaggaga cgctttccgg caaggagatc gaagaactgg
     4681 ttgcgcccga ggagccccgg ccggatgagc cgaagctccc gctggcttag gaggcgcatg
     4741 atcgacaaga agaagcttga aaaggcgacg cgccttttcc tcgagggcat cggcgaggac
     4801 ccggaccggc cggagctgaa ggagaccccg cggcgggtgg ccgagatggc cggcgagctt
     4861 tactccggcc tgggccggaa cccggccgag ctgctcaagg tggtcttcag cgaggagtac
     4921 gatgagctgg tcttcgtgcg tgacatcccc ttctactcga cctgcgagca ccacctgctg
     4981 cccttcctgg gcaaggccca catcgcctac ataccggagg ggcaccggat caccggcctg
     5041 tcgaagctgg cccgggtggt ggagaccctg agccgccggc cgcaggtcca ggagcggttg
     5101 accacccagg tggccgatac catcatgaag accctgcggc cgcgcggggt gatggtggtc
     5161 atcgaggccg agcacctctg catgacgatg cgcggcgtgc agaagcccgg ctgccaggcg
     5221 gtcacctcgg tcatgcgcgg catcttcctc gaggacgccc ggacccggaa cgaagccctc
     5281 aacctgctgc accgcgaccg ggtcggctga ggtacgatgg aacgacaggc ggaattccgg
     5341 ctgaagctcg aacgcctccg ggcgctggcc gccgaacggg gccgggccgg ggtgctgatc
     5401 aatatccagc ccaacttcgc ctgggcgacc gccggcggcg agaaccgggt cgggtcggcc
     5461 accgagaccg gcgccgccga cctgctggcg gttgacggcc gggcctacct gctggccagc
     5521 aacatcgagg ccggtcgggt gatggccgag gagctggccg gcctcggggt ggagccgctc
     5581 gcctatccct ggcacgaacc ggcggcccgg cgccagcggc tggccgaagt gaccgacccg
     5641 gaccggctgc tttccgac
//
LOCUS       DAOQYN010000127         5576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_14843_length_5576_cov_4.268430, whole genome shotgun sequence.
ACCESSION   DAOQYN010000127 DAOQYN010000000
VERSION     DAOQYN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14843_length_5576_cov_4.268430"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1806
                     /gene="ilvB"
                     /locus_tag="PKN80_05955"
     CDS             124..1806
                     /gene="ilvB"
                     /locus_tag="PKN80_05955"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNQ35591.1"
                     /translation="MKKKLTGAQIVLEALKKEGIQHIFGIPGGVLIPLMDALYDEPEI
                     KFMLTRHEQAAAHMADGFARATGRPAVCLATSGPGATNLVTGIATAHMDSIPMVAITG
                     QVATSLIGNDAFQEVDITGITRPITKNNYLVKDVRELAQVFREAFYVAASGRPGPVLI
                     DIPVDVQKASAEFDYPKEIELRSYKPKYEGHPIQIKKALELIAHSSRPIIYAGGGVVR
                     SRASAELTEFARMTGIPVILTLMGLGGFPAGDELFCGMPGMHGTVYANHSLMETDLII
                     AVGARFDDRVTGKVDTFAPHARIIHIDIDSSSIGKNVRVDVPIVGDIKHVMRQMLLEA
                     PKASCAPWRERIAEWKRKYPLEYKRDDKLHPQFIIEEINRLTGGEAIIATEVGQHQMW
                     VAQFYRFKEPDLLITSGGLGTMGFGFPAAIGAQIGRPDKVVFDIAGDGSFQMNIQELT
                     TAVENRIPVKVAVLNNCHLGMVRQWQELFYNKRYSGTILSPCTDFAAIAGAFGARGCR
                     VSRPEEVAPALEASLKEKEVPFVIDFRIEPDEKVFPMVPAGASLDEMLSGIS"
     gene            1823..2299
                     /gene="ilvN"
                     /locus_tag="PKN80_05960"
     CDS             1823..2299
                     /gene="ilvN"
                     /locus_tag="PKN80_05960"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNQ35592.1"
                     /translation="MEERVISLTVENRFGLLAKVAGLFSARGYNINSLVVAPTEDPEI
                     SRMVLTTSGDQRIIEQIIKQLNKVIGIIKVTDLCENDYLERELLLVKVNVANQKRTEI
                     IELAELFKARIIDVARKFLTIELVSETRKVDGFLELLKPYGLKEVLRTGTVAVSRE"
     gene            2409..3401
                     /gene="ilvC"
                     /locus_tag="PKN80_05965"
     CDS             2409..3401
                     /gene="ilvC"
                     /locus_tag="PKN80_05965"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNQ35593.1"
                     /translation="MAKIYYDKDANLKVLKDKTVAILGYGTQGRAHSLNLRDSGISVV
                     VAELPGTANYAKAVADKFKPVSAAEATRKADVIMMLVQDNLQQMVYQKDIQPNLKAGK
                     ALGFAHGFAIVYHQVVPPANIDVFMVAPKGPGGLVRDMFVQSKGVPCLVAVEQDHTGQ
                     ALKIALAYTKALGGSKAGIIETTFQEETETDLFGEQAVLCGGATALIKAGFETLVEAG
                     YQPEIAYYETCHELKLIVDLINERGLTGMRKGVSDTAEYGDLTRGPRVINDLVKEEMR
                     QILTEIQNGEFAREWILENQVGRPVYHALSKQDEKHPLEIVGQKLRGMMAWLKK"
     gene            3441..3992
                     /gene="thpR"
                     /locus_tag="PKN80_05970"
     CDS             3441..3992
                     /gene="thpR"
                     /locus_tag="PKN80_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNQ35594.1"
                     /translation="MRLFFAVTVDPAARAKIITGRPLENISSLRWVKPEQQHFTLCFL
                     GEESPDRLPKIIAAGKSAAGRHQAFRIRLRETGFFPERGDPRIFWVGVDQGASEIERL
                     AADLRAELRLRSLGFDARPFIPHLTLARINGRLTGPDRERLRGFSPGTALLPVPGFRL
                     LESRLAPDGASYFELASFELSEE"
     gene            4026..5015
                     /gene="recA"
                     /locus_tag="PKN80_05975"
     CDS             4026..5015
                     /gene="recA"
                     /locus_tag="PKN80_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNQ35595.1"
                     /translation="MEAKNKSGLNEAIKEIQKEFGKGSIMRLGERVAERIACIPTGAL
                     SLDVALGIGGIPRGRVTEIFGPESSGKTTLALHIIAEAQKSGGQAAFVDAEHALDPEY
                     AKKLGVDINNLLLSQPDSGEQALEIVDRLIKSNALDVVVVDSVAALVPRAELEGAMGD
                     SVMGLQARLMSQALRKLTSVINRTNTAALFLNQIREKIGVFFGNPETTPGGRALKFYT
                     SIRLDVRRIGSIESEKNIVGNHVRVKIVKNKVAPPFKEALFDIYFNQGISQEGCLLDL
                     GLEQKLLEKKGNSIYYQEQALGAGREQARLFLKENRPLREKLETELRGRLLTA"
     gene            5089..>5576
                     /locus_tag="PKN80_05980"
     CDS             5089..>5576
                     /locus_tag="PKN80_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNQ35596.1"
                     /translation="MTRRQWLSVLLTTVLVAGLASLLTVFLALKFLPSFPRRAAPPAY
                     SSGTDLAGLGNDITRVAEAVKPAVANISTTQRVRSSSPFDNFMQDPFFRKFFGDLFPA
                     PREHLTQSLGSGVLVSADGYILTTNHVIAEAAAVKVKLLDGREFNARVAGQDAKTDLA
                     GVV"
BASE COUNT         1162 a         1789 c         1680 g          945 t
ORIGIN      
        1 caatccgaag gccgagattt atctcgcctc gccggccacg gtggccgcaa cggccgtcgc
       61 cggcgaaata accgttccgg gatcggcttg atccagccaa tcccctgaag aatcacgacc
      121 aagatgaaaa agaaactgac cggcgcccag attgttctgg aggcgctcaa gaaagagggt
      181 atccagcata tcttcggcat tccgggcgga gttctgatac cgctgatgga tgccctttac
      241 gacgaaccgg aaataaagtt catgctcacc cggcacgaac aggcggccgc ccacatggcc
      301 gatggtttcg cccgggcgac cggccggccg gcggtctgcc tggccaccag cggccccgga
      361 gccaccaacc tggtcaccgg catcgccacc gcccacatgg attccatccc gatggtcgcg
      421 atcaccggcc aggtggccac ctcgctcatc ggcaacgatg cttttcaaga agtcgacatc
      481 accggcatca cccgcccgat caccaagaac aattacctgg tcaaggatgt ccgggagctg
      541 gcccaggtct tccgggaggc cttctacgtg gccgccagcg gccggcccgg cccggttctg
      601 atcgatatcc cggtcgatgt tcaaaaggcc tcagccgagt ttgactatcc aaaagagatc
      661 gaattgcgca gctataagcc caaatacgag gggcacccga tccagatcaa gaaggccctg
      721 gaactcatcg cccactcaag ccggccgatc atttacgccg gtggcggagt agtccgcagt
      781 cgggccagcg ccgaactgac cgaattcgcc cggatgaccg ggatcccggt tatcctgacc
      841 ctgatgggac tgggcggctt cccggccggc gacgaactct tctgcggcat gccgggcatg
      901 cacgggaccg tgtacgccaa ccattccctg atggagaccg acctgatcat agctgtcggg
      961 gcccgtttcg atgaccgggt caccggcaag gtggacacct tcgcgccgca cgcccggatt
     1021 atccacatcg atatcgattc ctccagcatc ggcaagaatg tccgggtgga cgtcccgatc
     1081 gtcggcgaca tcaaacatgt catgcgacag atgctgctcg aggctccgaa ggccagctgc
     1141 gccccgtggc gcgagcgaat cgccgagtgg aaacggaaat atccgctcga atataagcgt
     1201 gacgataagc ttcaccccca gttcatcatc gaggagatca accggcttac cggcggtgag
     1261 gcgatcatcg ccaccgaagt cggccagcac cagatgtggg tggcccagtt ttaccgtttc
     1321 aaagaaccgg atctgctgat tacctccggc ggcctgggca ccatgggttt tggtttcccg
     1381 gcggccatcg gcgcccagat cggccggccg gacaaggtcg ttttcgatat cgccggtgac
     1441 ggcagtttcc agatgaacat ccaggagctg accacggcgg tggaaaaccg gatcccggtc
     1501 aaagtggccg tcttgaacaa ctgccacctg ggcatggtgc gtcagtggca ggagcttttt
     1561 tataacaagc gctattccgg caccatcctg agcccctgca ccgatttcgc cgccatcgcc
     1621 ggcgccttcg gtgcccgggg ctgccgggtc agccgccccg aggaggtcgc cccggccctg
     1681 gaggcgtccc tcaaggaaaa agaagtgcct ttcgtgatcg atttccgcat cgagccggat
     1741 gaaaaggttt tcccgatggt tccggccggc gcttcgttgg acgagatgct gagcggcatc
     1801 tcctgaaaat ccgggagaca agatggaaga acgcgttatc agcctgaccg ttgagaaccg
     1861 cttcggcctc ctggccaagg tggccggact cttctcggcc cggggctata atatcaacag
     1921 cctggtggtg gcgccgaccg aggacccgga gatcagccgg atggttctca ccaccagcgg
     1981 cgaccagcgc atcatcgaac agatcatcaa gcagctgaac aaggtcatcg gcatcatcaa
     2041 ggtgaccgat ctctgcgaga atgattacct ggaaagggag ctgctcctgg tcaaggtcaa
     2101 cgtcgccaac cagaaacgga ccgagatcat cgagctggcc gaactcttca aggcccgcat
     2161 cattgacgtc gccagaaaat tcctgaccat cgagctggtc tcggagaccc ggaaggtgga
     2221 cggtttcctg gaattgctga agccctacgg cttgaaggaa gtcctgcgca ccgggacggt
     2281 ggccgtcagc cgggaatgaa aaatccggga tggccgccgg ccggccggcc cggcattaac
     2341 aggaaaattc ctgcaaaaaa gagcggtaga aaaccgtgag ggttttcctc acacaaaagg
     2401 aggcgcaagt ggctaagatt tattatgaca aggacgccaa cctgaaggtt ttgaaagaca
     2461 agacggtggc catcctcggt tacggaaccc agggacgggc ccacagcctg aacctccggg
     2521 actccggcat ctcagtcgtg gtggccgagc tgcccggcac cgccaattac gcgaaggccg
     2581 tggccgacaa gttcaagccg gtcagcgccg ccgaagccac ccgcaaggcc gacgtgatca
     2641 tgatgctggt tcaggacaac ctccagcaga tggtctacca gaaggacatc cagcccaacc
     2701 tcaaggccgg caaggccctg ggcttcgccc acgggttcgc gatcgtctac caccaggtgg
     2761 tcccgcccgc gaacatcgac gtcttcatgg tggcccccaa gggcccgggc ggcctggtgc
     2821 gcgacatgtt cgtccagtcc aagggtgttc cctgcctggt ggcggtggaa caggaccata
     2881 ccggccaggc gctcaagatc gccctggctt acaccaaggc cctgggcggc agcaaggccg
     2941 gcatcatcga gaccaccttc caggaggaga ccgaaaccga cctcttcggt gaacaggccg
     3001 ttctctgcgg cggcgccacc gcgctgatca aggccggctt cgaaaccctg gtggaggccg
     3061 ggtaccagcc tgaaatcgcc tactacgaga cctgccacga gttgaagctg atcgttgacc
     3121 tgatcaacga acgcggcctg accggcatgc gcaagggagt cagcgacacc gccgagtacg
     3181 gcgacctgac ccgcggtccc cgggtcatca acgacctggt caaggaagag atgcgccaga
     3241 tcctgaccga aatccagaac ggggaattcg cccgggagtg gatcctggaa aaccaggtgg
     3301 gacggccggt ttaccacgcg ctttccaagc aggatgaaaa gcatcccctg gaaatcgtcg
     3361 gccagaagct ccggggcatg atggcctggc ttaaaaaata aggcccttgc cgggccgccg
     3421 aacgggcggc tgatttttcc atgcgcctct tcttcgcggt gaccgttgat ccggccgccc
     3481 gggcaaagat tattaccggg cggccccttg aaaatatctc cagcctgcgc tgggtgaaac
     3541 cggagcagca gcacttcacc ctgtgtttcc tcggggaaga atctccggac cggctcccga
     3601 agatcattgc cgcgggaaaa tcggccgccg ggcgccacca ggccttccgg atccggctcc
     3661 gggagaccgg gttctttccc gagcgggggg accccaggat cttctgggtc ggcgtggatc
     3721 agggcgcgtc cgagatcgaa cggctggccg ccgacctgcg ggccgagctc cggctgcgtt
     3781 cgctgggctt cgacgcccgg cccttcatac cccatcttac gctggccagg atcaacggcc
     3841 ggctcaccgg gccggaccgg gaaagacttc gcgggttctc tccggggacc gcccttttgc
     3901 cggttcccgg cttccgtttg ctggaatcac gcctggcccc ggacggcgcc agctactttg
     3961 aattggccag cttcgaactg tccgaagagt agggggggcc tgttttctaa aaaaggaggc
     4021 gggtggtgga agccaagaac aaaagcggtc tcaacgaggc catcaaggag attcagaagg
     4081 agttcggcaa aggctcgatc atgcgcctgg gcgaacgggt ggccgagcgg atcgcctgca
     4141 tccccaccgg cgccctttcg ctcgacgtgg cccttggtat cgggggcatc ccgcgcggcc
     4201 gggttaccga gatcttcggc cccgaatcct ccggcaagac cacgctcgcc ctgcacatca
     4261 tcgccgaagc ccagaaatcg ggaggacagg cggccttcgt ggatgccgaa catgccctgg
     4321 accccgagta cgccaagaaa ctcggggtcg acatcaacaa cctgctgctt tcccagcccg
     4381 attccgggga gcaggccctc gagatcgtcg accgcctgat caagagcaac gccctggacg
     4441 tggtggtggt cgactcggtg gccgccctgg tcccgagggc cgaactggaa ggggccatgg
     4501 gcgacagcgt catgggcctc caggcgcggc tcatgagcca ggcgctccgc aagctgacca
     4561 gcgtcatcaa ccggaccaac accgccgccc tcttcctgaa ccagatccgc gaaaagatcg
     4621 gggtcttctt cgggaacccg gaaaccaccc caggcggccg ggcgctcaaa ttctacacca
     4681 gcatccgcct tgacgtccgc cgcatcggct cgatcgaatc cgaaaagaac atcgtgggca
     4741 accatgtccg ggtcaagata gtcaagaaca aggtggcccc tcccttcaag gaggccctct
     4801 tcgacattta tttcaaccag gggatctccc aggagggctg cctgctggac ctcggtctgg
     4861 aacagaagct gctcgaaaag aaaggaaact cgatttatta ccaggagcag gcgctcggcg
     4921 ccggacgtga acaggcacgc ctctttctca aggaaaaccg ccccctgcgg gagaagctgg
     4981 aaacggagct gcgcggccgc ctgttgaccg cctgaaaaga tggctgacgg ggaacccata
     5041 actcctggta acggcaaaga caaccttcct tccccaaaag gaggaacgat gactcgcaga
     5101 cagtggttat cggtcttgct gacgacggtc ctggtggccg gactggcctc cctgcttacc
     5161 gttttcctgg cccttaagtt cctgccctcc ttcccgcgcc gcgcggcgcc gccggcatat
     5221 tcttccggca ccgacctggc cgggctcgga aacgacatca cccgggtggc cgaagcggta
     5281 aagccggcgg tggccaacat cagcaccacc cagcgggtcc gttccagcag ccccttcgac
     5341 aacttcatgc aggacccgtt cttcaggaaa ttcttcgggg accttttccc ggcgccgcgc
     5401 gaacacctga cccaatccct cggaagcggc gtgctggtct cggccgacgg gtacatcctg
     5461 accactaacc acgtgatcgc ggaggccgcc gcggtgaagg tcaagctgct ggacggccgc
     5521 gaattcaacg cccgggtggc cggccaggac gccaagaccg acctggccgg cgtggt
//
LOCUS       DAOQYN010000128         5574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_14851_length_5574_cov_5.181011, whole genome shotgun sequence.
ACCESSION   DAOQYN010000128 DAOQYN010000000
VERSION     DAOQYN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14851_length_5574_cov_5.181011"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKN80_05985"
     CDS             complement(<1..392)
                     /locus_tag="PKN80_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNQ35597.1"
                     /translation="MKFSEEEYERLLVENQERIYRLALRIIGNREEALDVTQETLAAA
                     YQQREKFRGEAKISTYLYRIGLNYAFASLKKRKRLHLPIDSIGERPGNLDSPAQALAK
                     KERARRMELLLDGLPARQKAAVHLRIYD"
     gene            complement(440..1693)
                     /locus_tag="PKN80_05990"
     CDS             complement(440..1693)
                     /locus_tag="PKN80_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNQ35598.1"
                     /translation="MARDDEFIERVRERAAQREVRQVQESATAEDQTAASLLIKIIGD
                     AVHAKATDIHLEVEETGPKLRYRVNGLLYDFPAPAREIYARLLARIKVLSDLDVTERR
                     LPQSGYCSFNAGDRRVDLRISTFPSVFGESAAIRVLDRNNIVLGFDRLGFQPEVLAQY
                     LKILEFPYGMILVTGPTGGGKTTTLYTSLNKIRSGRKKIITLEDPVEYHIPGVTQGQI
                     NIKAGFTFAEGLRSILRQDPNVVMIGEIRDGETANTAMQISLTGQLVFATLHTNTAPG
                     AVTRLLEMGLEPYLVSSSLLGVINQTLVPSVCQQCKAEYRPEAEVLAEAADDLKRFPG
                     LKFFRGQGCPRCNQTGVQGRLGLFELMVINRRLRELILSKPSVEAVTSAALEAGMLTL
                     REDGLRKAAAGLAKLEDVVRITRRE"
     gene            1822..2433
                     /gene="purN"
                     /locus_tag="PKN80_05995"
     CDS             1822..2433
                     /gene="purN"
                     /locus_tag="PKN80_05995"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNQ35599.1"
                     /translation="MLKIGVLISGNGSNLQALIDEVKAGRLPAEIALVLSDNPEAYGL
                     KRAAAAAIPARFLPPGPKRTVLSPEAEAAYADAFKQAGVELVCLAGFMRVIKAGLLAA
                     FPGRILNIHPSLLPSFPGLAAWKQALDYGVRYTGCTVHLVEAGVDTGPIILQAVVPVE
                     ANDTPETLLNRIHREEHRIYPEAVRLFAESRVRLDGRRAVVTD"
     gene            2463..4364
                     /locus_tag="PKN80_06000"
     CDS             2463..4364
                     /locus_tag="PKN80_06000"
                     /inference="COORDINATES: protein motif:HMM:NF037720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNQ35600.1"
                     /translation="MSPKICFVNIEKAPAELRAGLKALGTEYPVALKPAAGAFRVDFR
                     PANSESTGPDLEVRPGREGLTVTYRRPIHAFRALGRLLAAPAGKKQPGFSETAACRTA
                     GVMWECSRNGVLTLEAAKALLRRMALMGLNLAMLYTEDTYEVPGEPFFGYLRGRYTRR
                     ELKEIDDYAAALGIEMIPCIQALGHLAQILQWSEAYRSVTDTSRILLVGADQTYRLLE
                     KMIAAASAPFRSRRIHVGMDEAHGLGTGYYKTLNGERRTFDIMTEHLKRVVEICRKQG
                     LQPMMWSDMFFRIGSARNSYYDLEARIPDEVAGEIPAEMDLVYWDYYNTDPEIYRRMI
                     TRHRSMGKDPIVAPGAWNWNRFWPALPFAFKTLDVCLGVCREEKIKEIFITTWGDNGM
                     ENDTFSTLPALQYFAEHIYQAKFDRRLFERNFRAAGRGEISDYLLASRLDYLPGHPGP
                     ETLPGNHSKRILWDDPLLGLCEPLLGRKSLRPHYRDLAEKLATAARQPGQERLDFPAQ
                     LARTLALKCDCRKNLAAAYRAGDRRRLKRLLKEEVQPLRREMEKLWKTHRRIWLDTYK
                     PFGLEALEIRYGGQLVRLQTLLDRLQDYLAGRTGSIPELETRLLAFTASPQPTLGYRR
                     IATPTADPG"
     gene            4453..4938
                     /locus_tag="PKN80_06005"
     CDS             4453..4938
                     /locus_tag="PKN80_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNQ35601.1"
                     /translation="MAATKKRKLKIRLRKARLADAKNIHALINLAAREGAVIPRSLSE
                     IYESIRDFQIAEADRRLVGCCALHIDWEDLAEIRSLVIDPEHQRIGLGSRLLKSALKE
                     ARELLVPRVFALTAHPAFFEKNGFERIRRGRLPHKIWGDCLKCPKFPKCDEVALWKKL
                     S"
     gene            4920..>5574
                     /locus_tag="PKN80_06010"
     CDS             4920..>5574
                     /locus_tag="PKN80_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNQ35602.1"
                     /translation="MEKAVLAYSGGLDTSVIIPYLREKYRLQVIAYTANLGQKIDPDF
                     LRKKALASGAAGFYFDDLRETFVRDYIFPALAANALYQGRYPLATALSRPLIARRVVE
                     VARREKAKFLSHGCTGKGNDQVRFELTWKALGPDFEIIAPLREWQFKTRDEEIEFARQ
                     RKIPVPVTRKSPYSLDENLWGVSIECGILEDPAVVPPEDAYQITTAPVPGFDEARFTI
                     "
BASE COUNT         1039 a         1844 c         1716 g          975 t
ORIGIN      
        1 tcatcgtaaa ttctcaggtg aaccgccgct ttctggcggg ccggcaaccc gtccaggagc
       61 agttccatgc gccgggcccg ctccttcttg gccagcgcct gggccgggct gtcgagattc
      121 ccgggccgct cgccgatgct gtcgatcggc aggtgcaacc gcttcctctt tttcagcgag
      181 gcgaaggcgt aattaagacc gatccggtag agataggttg aaatcttcgc ctcgccccgg
      241 aacttctcac gctgctggta ggcggccgcc agggtctcct gggtaacgtc cagggcctct
      301 tcgcggttgc ctatgattct cagggccaac cggtaaatgc gctcctggtt ttcaaccagg
      361 agccgctcgt attcttcttc ggaaaacttc atcttattag atgggctgac cgcggccagc
      421 gtttaaaaat cgattacctt tattcccggc gggtgatcct gacgacatcc tccagcttgg
      481 ccaggccggc cgccgccttg cgcaggccgt cctcgcgcag ggtgagcatg ccggcctcca
      541 gggccgcgga agtcaccgct tcgaccgaag gcttggaaag tatgagctcc cgcagccggc
      601 ggttgatgac catcagctcg aaaaggccca ggcgcccctg gactccggtc tggttgcacc
      661 gggggcaacc ttggccccgg aagaatttca acccgggaaa acgtttcagg tcgtcggccg
      721 cctcggccag aacctcggcc tccggccggt actcggcctt gcattgctgg caaacgctgg
      781 gaaccagcgt ctggttgatc acgcccagga gcgaggagct caccaggtag ggttccagtc
      841 ccatttcgag cagccgggtc accgccccgg gcgcggtgtt ggtatgcagg gtggcgaaga
      901 ccagctgccc ggtaagcgat atctgcatgg ccgtgttggc cgtttcgccg tcccggattt
      961 cgccgatcat caccacgttc gggtcctggc ggagtatcga acggagtccc tcggcaaagg
     1021 tgaagccggc cttgatgttg atctgcccct gggtcacacc cgggatgtgg tattcgaccg
     1081 ggtcctcgag cgtgatgatt ttcttgcggc cggagcgaat cttgttgagc gaggtgtaga
     1141 gggtggtcgt cttgccgccg ccggtcggac cggtgaccag gatcatcccg tacgggaatt
     1201 cgagaatctt gaggtactgg gccagcacct cgggctgaaa accgagccgg tcgaatccca
     1261 gcacgatgtt gttccgatcc aggacccgga tggcggcgct ttcgccgaaa acggacggaa
     1321 aggtcgagat gcgcaggtcg acccgccggt caccggcgtt aaaggagcag taaccactct
     1381 ggggcagacg cctttcggtg acgtccaggt cggaaagcac cttgatgcgg gccagcaggc
     1441 gggcgtagat ttcgcgggcc ggcgccggaa aatcgtagag cagcccgttc acccggtagc
     1501 gcaatttcgg accggtctcc tcaacctcga gatggatatc ggtcgccttg gcgtggaccg
     1561 catcaccgat gatcttgatc agcagggagg cggcggtctg atcctcggcc gtggccgact
     1621 cctggacctg ccggacctcc cgctgggcgg cccgttccct tacccgttct atgaactcgt
     1681 catccctggc catatctttt accccggtcg gttgaaggag acggaattcc agagcagtta
     1741 tattttagca taacggagag gccgaaacct ccggaagcgc ttttttattt tgaataaaga
     1801 tatgtgttaa tatagattat catgttgaaa atcggtgttt taatctccgg aaacggctcc
     1861 aacctgcagg ccctcatcga cgaggtgaag gccggccggc tgccggccga aatcgccctg
     1921 gtgctgtcgg acaacccgga agcctacgga ttgaagcggg cggccgcggc cgccatcccg
     1981 gcccgttttc tgccgccggg tccgaagcgg acggtcctgt cgccggaggc cgaagcggct
     2041 tacgccgacg cttttaaaca ggccggcgtc gaactggttt gcctggccgg cttcatgcgg
     2101 gtgatcaagg ccgggctcct ggccgccttc ccggggcgca tcctgaacat ccatccctcg
     2161 ctgctaccct ccttcccggg actggccgcc tggaaacagg ccctggatta cggggtccgc
     2221 tacaccggct gcaccgtcca tctggtcgag gccggggtcg acaccggacc gatcattctg
     2281 caggcggtgg tcccggtcga ggcaaacgat accccggaga ccctgttgaa ccgcatccac
     2341 cgcgaggaac accgcatcta tcccgaagcc gtccgtctct tcgccgaaag ccgggtccgg
     2401 ctcgacggcc gccgggccgt cgtcaccgac tgaatcatcc gaaaccagtt aaaggagcaa
     2461 ggatgagccc aaaaatctgt ttcgtcaata ttgaaaaggc cccggccgaa ttgcgggccg
     2521 gactcaaggc cctgggcacc gaatacccgg tcgccctgaa accggccgcc ggcgccttcc
     2581 gggtcgactt ccggccggcc aactccgagt ccaccggccc cgatctggag gtccggcccg
     2641 gccgggaggg tctcacggtc acctaccggc gcccgattca cgctttccgg gccctgggcc
     2701 gactgctggc cgccccggcc ggaaaaaagc agccgggctt cagcgaaacc gccgcctgcc
     2761 ggaccgccgg cgtgatgtgg gaatgctccc gcaacggcgt gctgaccttg gaggccgcca
     2821 aggccctcct gcgccggatg gcactgatgg gcctgaatct ggccatgctc tacaccgagg
     2881 atacctacga ggtgccgggc gagccgttct tcggttacct gcgcggccgc tacacgcgcc
     2941 gggagctcaa ggagatcgac gattacgcgg cggccctggg catcgagatg atcccctgca
     3001 tccaggcgct gggccacctg gcccagatcc tccagtggag cgaggcctac cggtcggtga
     3061 cggacacctc ccggatcctg ctggtcggcg ccgaccagac ctatcgcctg ctggagaaga
     3121 tgatcgcggc cgccagcgcg cctttccggt cgcgccggat ccacgtcggc atggacgagg
     3181 cgcacggcct cggcaccggc tattacaaga ccctcaacgg ggaacgccgc accttcgaca
     3241 tcatgaccga acacctgaag cgggtcgtgg agatctgccg gaaacagggg ctgcagccga
     3301 tgatgtggag cgacatgttc ttccggatcg ggtcggcccg caactcctat tacgacctcg
     3361 aggcccgaat cccggacgag gtggccgggg agatcccggc cgagatggac ctggtctact
     3421 gggactatta caacaccgac ccggaaattt accgccggat gatcacccgc caccgctcga
     3481 tgggcaagga cccgatcgtg gccccgggcg cctggaactg gaaccgtttc tggccggcgc
     3541 tgcccttcgc cttcaaaacc ctggacgtct gcctgggcgt ctgccgtgag gagaagatca
     3601 aagagatatt catcaccacc tggggtgaca acggcatgga gaacgatacc ttctcgaccc
     3661 tgccggccct gcagtatttc gccgagcaca tctaccaggc taaattcgac cggcgcctct
     3721 tcgaacggaa cttccgggcg gccggccgcg gggaaatttc cgactacctg ctggccagcc
     3781 ggctcgatta cctgcccggc caccccgggc cggaaaccct gccgggcaac cattccaagc
     3841 ggattctctg ggatgatccg ctcctcggcc tctgcgaacc gctcctgggc aggaagagcc
     3901 tgcgtccgca ctaccgggac ctggctgaaa aactggcgac cgcggcccgc cagccgggcc
     3961 aggaacgcct ggacttcccg gcccagctgg cccggacgct ggccttgaaa tgcgactgtc
     4021 ggaagaacct ggccgcggct taccgggccg gcgaccgacg gcgactaaag cgcctgttga
     4081 aggaggaagt ccagccgctc cggcgcgaaa tggaaaaact ctggaagacg caccgccgga
     4141 tctggctgga cacctacaag cccttcgggc tggaggccct tgaaatccgc tacggcgggc
     4201 agctggtccg gcttcagacc ctcctggacc gactccagga ttacctggcc ggccggaccg
     4261 ggtcgatccc ggagctggag acccgcctgc tggccttcac cgccagcccc cagccgacgc
     4321 tgggctaccg gcgcatcgcc acgccgaccg ccgatccggg ttaagggcgg cggcgcggca
     4381 tcagacaagc aaacgggacc gagcggtccc ggcggagttg cagtaaccag ggttaagttc
     4441 ccgggagaag agatggcggc taccaagaaa aggaaactga aaatacggtt gcggaaagcg
     4501 cggctggccg acgccaagaa cattcatgcg ctgatcaacc tggccgcccg cgagggtgcg
     4561 gtcattcccc gctccctgtc ggaaatctac gaatcgatcc gggatttcca gatcgccgag
     4621 gccgaccgcc ggctggtcgg ctgctgcgcc ctgcacatcg actgggagga cctggccgaa
     4681 atccgctcgc tggtaatcga ccccgaacac cagcgaatcg gactgggcag ccgactcctc
     4741 aagtcggccc tgaaagaggc ccgggaactc ctggtgccca gggtcttcgc cctgaccgcc
     4801 cacccggcct tcttcgagaa gaacggtttt gaacgcatcc gcaggggccg gctgccccac
     4861 aagatctggg gcgactgcct caagtgtccc aaattcccca aatgcgacga ggtggcttta
     4921 tggaaaaagc tgtcttagct tactccggcg gactcgacac atcggtcatc atcccttacc
     4981 tccgggaaaa ataccggctc caggtgattg cctacacggc caacctgggc cagaagatcg
     5041 acccggactt tctccgcaag aaagcactgg ccagcggagc ggccggcttc tatttcgacg
     5101 acctgcgcga gactttcgtg cgcgactaca tcttccccgc cctggcggcc aacgccctct
     5161 accagggacg ctacccgctg gccaccgccc tctcccgccc gctgatcgcc aggcgggtgg
     5221 tcgaagtggc ccgccgggaa aaagccaaat tcctgagcca cggctgcacc ggaaagggca
     5281 acgaccaggt ccgctttgaa ctgacctgga aggcgctcgg cccggacttc gagatcatcg
     5341 cgcccctgcg cgaatggcag ttcaagaccc gcgatgagga gattgaattc gcccggcagc
     5401 gcaagatacc ggtcccggtg accaggaaga gtccctactc cctggacgaa aacctctggg
     5461 gcgtctccat cgaatgcggc atcctggagg acccggcggt cgttccgccc gaggacgcct
     5521 accagatcac caccgccccc gtgcccggtt ttgacgaggc ccgcttcacc atcg
//
LOCUS       DAOQYN010000129         5522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_15021_length_5522_cov_3.821108, whole genome shotgun sequence.
ACCESSION   DAOQYN010000129 DAOQYN010000000
VERSION     DAOQYN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15021_length_5522_cov_3.821108"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1074)
                     /locus_tag="PKN80_06015"
     CDS             complement(61..1074)
                     /locus_tag="PKN80_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35603.1"
                     /translation="MAIKVAVVGCGYWGPNLARNFSQLAGTELAALCDRDRTRLAGLG
                     RIYPAARLFTDYRKLLAWPEIQAVALATPVATHYRLGRLALEQGRDLLVEKPLTASGR
                     EAESLIRLAREKGRLLMVDHTFEYAPAINRMREIIAAGELGDLYYIRAEWLNLGLLQP
                     DVNVIWDLATHIVSILNYVTGLEPSSLKATAAAYLRREIPEVAHIQLNYAGQVRAFMT
                     VSWLEPRKTRSITIVGKRRMLVYDLTNTEEQIKIFDKGVDLDASHSEANQWRINYRYG
                     DIYSPNLKNVEPLSALCGHFADCLKDRRRPRSDGESGARVVRVLEAAQRSLRRNGREI
                     KLS"
     gene            complement(1097..2155)
                     /locus_tag="PKN80_06020"
     CDS             complement(1097..2155)
                     /locus_tag="PKN80_06020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ35604.1"
                     /translation="MRLFNRPLPRSLYPAFLAAVYALLGKGFLAVRIIQAVLGAVTCG
                     LVFLLARRIFDSPRAGILAALAAVVSPALVAAASYVLTETLATFLLIGAVFLLLSGLD
                     KQCRLDWWLAGILFGLASLVRPSILLLPFGLAVLLPAALEKARRPALAAGLMLGTLLA
                     VAPWTARNYKVFHRFIPIGLGGGFNLWAGSYAPWDGDYNWRDLGAAEDLVAGLSQVEA
                     DRKYSSEAIKNIRANPGFYARLAFRKIGRFWLQAPGSQQVLAGWPAARGLVYLAHYLT
                     LLLAAAGGAAAILRRRREAWLPFLVILYFTGIHVLMLALPRYHIPALPMVAVLAGGAA
                     AGGGSSLARPGPETRPDA"
     gene            complement(2334..2951)
                     /locus_tag="PKN80_06025"
     CDS             complement(2334..2951)
                     /locus_tag="PKN80_06025"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HNQ35605.1"
                     /translation="MRKCLTGLFLLVLAAGCATVNVYITFPQEKLEKAAEQIELDLQK
                     DLMLTPPPAAPRSGQSRVNPLKWLGPAPAYAQTISSEVKTRSPLIGEIMKQRRERLSD
                     IQVFKDKGTLGEDREGLLDVRSSTGLDRESRLKQEQLVKEENQDRLAMYREIVKINSM
                     PSAELKNVQAAFAKIQRKLAQVGEWIQEEDGTWTQKKEVPDKKNK"
     gene            complement(2965..4482)
                     /locus_tag="PKN80_06030"
     CDS             complement(2965..4482)
                     /locus_tag="PKN80_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35606.1"
                     /translation="MRSEKKRRIRLAALVLVAVGALWTGFHLPELAVRYVGWRVWFGS
                     RGKVLLKETVLLPGPGLAIGEVVLTDWQKPPAAVSIRDLTIQRLPTAGSGAPLVIALN
                     GKGSLGAARPHPLKITGRITGDPAAGNYQFEKIRIRIDRLGEVTLSGRLAVRGDSSQF
                     SAHLADVSLAELSRAIDLPQLPTGGVVRGDLDFYLRKEKEVWQLQKVGGDLSFEGLQL
                     SQAGLPPLSGSVAGSYDMSGRSGEITRASLKTPAGGQLSVNGRVGERGVDLSFTSEGL
                     YLEEIIQALPEPVRQKLGLKLENLAGRVNYEPEQRLFTAHVDGNFHQARLNGDLNGNF
                     PAGWPPDFLGRLDLEGLDLLAISRLYGEGAEISGLADLNLNFKAEQNQLTFLKARLET
                     IPNKSFRQYLNVGALRALLALSSGAVIRHFAASDYGYRKLATAVTYQDGYLTIEGLAR
                     AGRDRDYIMLGGWLGNTVNIAVDHRFNTVSLEELKRRFQQLAEPGGVQVGQQGIS"
     gene            4817..>5522
                     /locus_tag="PKN80_06035"
     CDS             4817..>5522
                     /locus_tag="PKN80_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ35607.1"
                     /translation="MEIDPVCQSVLQDLEEAALVCDLEFKIIYVNPPFEKMTGLEAAA
                     VLNLPGPDILTSGEDLLALLAGQRDDSRETAVRQPVFIKTARGRREALLTVITHRSGP
                     EQNGFILFLHEVRPETDPLTASSRTLRKVRKEFHHQADFQRQVLENEARYRSFVENFR
                     GIVFQGDKNCRPIFFHGAVEQLTGYTEADFRSGRPSWDKIVHPEDQPAFYEDAEKLFR
                     PGGAVEREYRIQQRDGG"
BASE COUNT         1042 a         1855 c         1672 g          953 t
ORIGIN      
        1 ggttgaccag gtcggggtga aaaatcaggg tgccccggcc gaccaccacg tcttccctga
       61 tcatgaaagt tttatctccc ggccgttccg gcgcagcgaa cgctgggcgg cctccagcac
      121 ccggaccacc cgggcgcccg actcgccgtc gctccgcggc cggcgccgat ccttcagaca
      181 atcggcgaag tggccgcaga gggcgctgag cggctccacg ttcttgaggt tgggggaata
      241 gatgtccccg taacggtaat tgatgcgcca ctggttggcc tcggaatggc tggcgtcaag
      301 atccacgccc ttgtcgaaga tcttgatctg ttcctcggtg ttggtcaggt cgtaaaccag
      361 catccgccgc tttcccacga tggtgatgct gcgcgtcttg cgcggttcca gccagctgac
      421 cgtcatgaag gcccggacct ggccggcgta attcaactgg atgtgggcca cctccggtat
      481 ctcccgacgc aggtaggcgg ccgcggtcgc cttcaggctc gagggttcga ggccggtcac
      541 gtaattcagg atggagacga tgtgcgtggc caggtcccag atcacgttca cgtcgggttg
      601 cagcaggccc aggttgagcc attcggcccg gatgtaataa agatcgccca gttccccggc
      661 ggcgattatc tcgcgcatcc ggttgatggc cggggcatac tcgaaggtgt ggtccaccat
      721 cagcagccgt cccttctccc gggccaggcg gatcagggac tcggcctcgc ggccggaggc
      781 ggtcaggggc ttctccacca gcagatcccg accctgttcg agggccaacc ggcccagccg
      841 gtaatgggtg gccaccggcg tggccagcgc caccgcctgg atctccggcc aggccagcag
      901 cttccgatag tcagtgaaga ggcgcgccgc cgggtagatc ctgcccagtc cggccagccg
      961 ggtgcggtca cggtcgcaga gggcggccag ctcggtaccg gccagctggc tgaagttgcg
     1021 ggccaggttc ggaccccagt atccgcagcc aaccaccgcc acttttatcg ccatcgtttc
     1081 tcccccgctc ttcgcgctaa gcatccggcc tggtttccgg ccccggcctg gcaaggctcg
     1141 atccgccgcc ggccgcggcc ccgccggcca gcaccgccac catcggcagc gccggtatgt
     1201 ggtagcgggg cagcgccagc atcagcacgt ggatgccggt gaaatagagt atcaccagga
     1261 atggcagcca ggcttcgcgg cggcgccgaa ggatcgcggc ggctccgccg gccgcggcca
     1321 gcagcagggt caggtaatgg gccaggtaaa ccagcccccg ggcggccggc cagccggcca
     1381 gcacctgctg gctgccgggc gcctgaagcc agaaccggcc gatcttcctg aaggccagcc
     1441 gggcataaaa gccggggttg gcccggatat tcttaatcgc ttcggaactg tacttccgat
     1501 ccgcctcgac ctgggaaagg cccgccacca gatcctcggc ggcgcccagg tcgcgccagt
     1561 tgtaatcgcc atcccagggc gcgtagctgc cggcccagag gttgaacccg ccgcccagcc
     1621 cgatcggaat gaaacggtgg aaaaccttgt aattgcgggc cgtccagggc gccaccgcca
     1681 ggagcgtgcc cagcatcaag ccggccgcga gggccggccg ccgcgctttt tcgagcgccg
     1741 ccggcagaag aaccgccagg ccgaagggca gcagcaggat cgacggccgg acgagactgg
     1801 ccaggccgaa gagtatcccg gccagccacc agtccaggcg gcactgctta tccagccctg
     1861 aaagcagcag gaagaccgct ccgatcaaca ggaaggtggc cagggtctcg gtcaggacgt
     1921 aggaagcggc ggccaccagc gccggcgaaa cgaccgccgc gagggcggcc aggatgccgg
     1981 cccgcgggga atcgaaaatg cggcgcgcca ggagaaagac cagaccgcag gtaaccgcgc
     2041 ccagcaccgc ctggataatc cggaccgcca aaaacccctt gcccagcagc gcgtaaaccg
     2101 ccgccaggaa agccgggtaa agggagcgcg gtagaggtcg gttgaaaagc cggatccgtt
     2161 cagaaggccc agggcgtaag cctggtaatc ggccgtgtcc ggcgcctcca gcggaagcag
     2221 cagcacgaag ccgatcctga tcaacagggc ggccaggaaa accgcccaga agattcttcc
     2281 ccctgaaatt ttcataacgc cggcggccgg cggccccccg ggagcccggc gcctcactta
     2341 ttcttcttgt cgggaacttc cttcttctga gtccaggtac cgtcctcctc ctgaatccat
     2401 tcgccgacct gggccagctt cctctggatc ttggcgaaag cggcctgaac gttcttcaac
     2461 tccgcggagg gcatgctgtt gatcttgacg atttcccggt acatcgccag ccggtcctgg
     2521 ttctcctcct tgaccagctg ctcctgtttc aaacggctct cccggtcaag gccggtgctg
     2581 gaccggacgt ccaacaaccc ctcccggtcc tcgccgagcg tacccttatc cttgaagacc
     2641 tggatatcgg acaggcgttc ccggcgctgt ttcatgatct ccccgatcag gggcgaccgg
     2701 gtcttgacct cggaacttat ggtctgggcg taggccggcg ccgggcccag ccatttcagc
     2761 gggttgaccc ggctctggcc gctgcgaggc gcggccggcg gcggggtcag catcagatcc
     2821 ttctgaaggt cgagctcgat ctgttcggcc gccttctcaa gcttctcctg cgggaaggtt
     2881 atgtaaacgt tcacggtggc gcacccggcc gccagcacca gcaaaaacaa gcctgtcaaa
     2941 catttgcgca tggtatcctc ctcttcagga aatcccctgt tgtccaacct ggacgccgcc
     3001 gggttcggcc agctgctgaa agcgccgctt caattcctcc aggctcaccg tgttgaaccg
     3061 gtggtccacc gcgatattga ccgtgttgcc cagccagccg cccagcatga tgtaatcccg
     3121 gtcccggcct gcccgggcca ggccttcgat ggtcaggtag ccgtcctggt aagtcaccgc
     3181 cgtcgccagc ttccggtacc cgtaatcgga ggcggcaaag tggcggatga ccgccccgct
     3241 gctcagggcc agcagggcgc gcagggcgcc cacgttgaga tactgccgga aacttttgtt
     3301 cggaatcgtc tccagcctcg ccttcaggaa agttagttga ttctgctcgg ccttaaagtt
     3361 tagattcagg tcggccagcc cggagatctc ggccccctcg ccgtaaaggc gggaaatcgc
     3421 aagcaggtcg agtccttcga ggtcaagccg gcccaggaaa tcgggcggcc agccggccgg
     3481 aaaatttcca ttcaggtcgc cgttaagccg ggcctggtga aaattcccgt cgacatgggc
     3541 ggtgaagagc cgctgctccg gttcgtagtt gacccgacca gccaggtttt ccagtttcag
     3601 acccagtttc tgccttaccg gctccggcag cgcctggatg atctcctcca ggtagagccc
     3661 ctccgaggtg aaggaaaggt caacgccccg ctcgccaacc cggccgttaa ccgaaagctg
     3721 ccctccggcc ggcgtcttca aactggcccg ggtaatctcc ccggatcgac ccgacatgtc
     3781 gtaggaaccg gccaccgatc cgctcagggg cggcagaccg gcctggctca gctgcaggcc
     3841 ttcaaaacta aggtcgccac ccaccttctg caattgccag acctccttct ccttcctcag
     3901 gtaaaaatcc aggtcgcccc gcaccacgcc cccggtcggc agctggggca ggtcgatagc
     3961 ccggctcaac tcggccagcg agacatcggc caggtgggcc gaaaactggc tgctatcccc
     4021 gcggacggcc agccggccgg aaagggtgac ctcaccgagc cggtcaatcc gtatccgaat
     4081 cttctcgaac tggtaattgc cggcggccgg atcaccggtg atgcggccgg taatcttcaa
     4141 agggtgcggg cgagccgccc ccagcgaacc cttgccgttc agggcgatca ccagcggcgc
     4201 cccggatccg gccgttggaa gccgctggat ggtcaggtcc cggatggaga cggccgccgg
     4261 cggtttctgc cagtcggtca ggaccacctc gccaatcgcc aggccgggcc cgggcagaag
     4321 caccgtctcc ttcaacagca ccttgccccg gctgccgaac cagacccgcc agcccacgta
     4381 gcggaccgcc agttccggca ggtggaagcc ggtccagagg gctccaaccg caacgagcac
     4441 gagagcggcc agccggatcc gtcttttctt ttcggacctc acaattaccc tcccccggta
     4501 gtatttatca gggtttatgg tattaaataa tacctttttc ataataacac attgaactcg
     4561 ggacaggcaa aaggaatcgg gcgcggacaa ttttcgtcaa ccccggccgg aaattgtacg
     4621 ccggtcccgc gccgatcctc cgaacctgaa gccccgcaaa tcccgtcggg cctgacctcc
     4681 ggcctttttc aaaccgatcg attcccgctg gcaaataaat tgcatcactc gtttcaggta
     4741 tacttgacct tatggagtat aatgtaatcg gcaagccatt atctcaattt cacgacaacc
     4801 ttcgcgggga atcagcatgg aaatcgatcc cgtctgccaa agcgttctcc aggacctgga
     4861 agaagccgcc ctggtctgcg acctcgagtt taaaatcatc tacgtcaacc ccccttttga
     4921 aaagatgacc ggcctggagg cggccgcggt cctcaacctc cccggcccgg acattctcac
     4981 ttccggtgaa gacctgctgg cacttctggc cggccagaga gacgattcca gggaaacggc
     5041 cgtccgccag ccggtcttca tcaagaccgc caggggccgg cgggaggctc tgctgaccgt
     5101 gatcacgcac cgctccggtc cggaacaaaa cgggtttatt ctcttcctgc acgaagtccg
     5161 gccggagacc gaccccctca cggccagttc ccggaccctg cggaaagtaa ggaaggagtt
     5221 ccaccaccag gccgattttc aaaggcaggt gctggaaaac gaggcgcgtt accggtcctt
     5281 cgtggaaaac ttccggggca ttgttttcca gggtgacaag aattgccggc ccatcttctt
     5341 tcacggcgcg gtcgaacaac tgaccggcta taccgaggcc gacttccgga gcggccggcc
     5401 cagctgggat aagatagtcc accccgagga ccagcccgcc ttctacgagg atgcggaaaa
     5461 actcttccgg cccggcggcg ccgtcgaacg ggagtaccgc atccagcagc gcgacggcgg
     5521 gg
//
LOCUS       DAOQYN010000130         5434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_15338_length_5434_cov_4.087377, whole genome shotgun sequence.
ACCESSION   DAOQYN010000130 DAOQYN010000000
VERSION     DAOQYN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15338_length_5434_cov_4.087377"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..2421
                     /locus_tag="PKN80_06040"
     CDS             160..2421
                     /locus_tag="PKN80_06040"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNQ35608.1"
                     /translation="MAKEFEWHKAGHDRRLKEIGSRIWFGTQYYRPPQPLPRFWESDF
                     NLMKKAGLEVARAWIYWRWHEREPGRFQFEELDRLFELGRRHGVFIHPLLFLESAPEW
                     FIRQFPETWFVDHQGVSYYPLNRKSTQIGGMAVCGNHPLTKEWSGRFLTALVNRYREH
                     PALLCWDVWNELQTKSVEGICACHHCRADYRSHLQQRYASLGELNAALNTGYGTWEDV
                     LPPNGNNDYAAWFAWRRWEASSLSRQALWRARTIWAADPRQVVMIHMPTKLSVPGHGC
                     GDWNDCAPYLDFAGNSSHIQRSHRGYSLPERLSARNLLAAEHDIAVQQSPNFAWAGEV
                     SSDTASYDAPPPLHPDELAYWTWKPISLGMRGVFYWQFRPEAYGPESPDLGLLELDGR
                     ASNRWERVGEIVKLVRENEPLFRSARPPVPQVGILTCPEQHMACATLYSKNSGGQQLF
                     QKSVLNLYDVFRSAGIPAAFVDPDRIGSAIKLLLVPRSPAGRPELKAGLLKFLEGGGT
                     AVVEGGFMSADGASFILEETAPGLGLDRVFGLREGRPESTRWHGLPTGSEESRLSKQE
                     LDDAWLDAGVDRETERSREKLAPELRISLAWGGREYPVTAGFDRRPLRAAGAEIIGRF
                     ASGEAALATRRVGKGRLIYIGTAASYVDRIFGGGYGRFLRALAGELAIRPPLPIPPAA
                     GATWRVLEAGARRLLFCFNHLETPVGFDLSRCGPARLIYGPGVELKGGRLSVGPLATA
                     VLELKNPTKEKSR"
     gene            2418..4643
                     /locus_tag="PKN80_06045"
     CDS             2418..4643
                     /locus_tag="PKN80_06045"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HNQ35609.1"
                     /translation="MKTTYPDWAHGMRLVTWSISAADLDLITPAEADRRAKRLRREGI
                     NAVVSFGAHFRWDWIKDFPRIREVTRISVEACHRQGIKYIEHHSACFVSRIPERTPEA
                     FEWFNRHLPNHTVPEPLALDAVVEYRGKRLNDWYEMNLKAGGPEYWQGYKAYCLCPNH
                     PEYRQAYFDYLEDFLKYTDADGIMSDDVTMGLNPYACVCGECRRLFKSETGYDLPERA
                     DDPFWSEFNRPDYRAWLDFRRNSVLRHYEKIDRMIQGWSKPLLHTSCATTISSPVSAE
                     TYRRQDVFNTTFVEVLSANPYYYSWMMISPELRIYRARARGGANPSFAIFYPQAKGGE
                     QLFGSAYLRQEEHSVWLSPNIGRHSPQTVGRDSEWALWEEKHDDLFRTAGTLTDLAVF
                     FSDASRRRYRGHHHEHYTYEFKGWCELLHEAGLDYDVVLEEDLAAGRLGQYRTLLAPN
                     AACLSPELSRAIEEYVRSGGRLLASHEASLFNFEGDPLENFQLAECLGLDYERTVFDE
                     HYPIQGWNGDDAFLAGVSGLIAHRAPQVRVRPRPGAEVLLTSFIDAGGRKGEIIPALV
                     SNRFGRGLARYFAGKPGILCYHPGIRARNWDGEEPYRFVNYRDPLLARVFLNAVRLTA
                     GSFRLELPNAKPGVFCRGYRLRDGRLAVHILNAAGSIIPHRTLVQPGYRVKYPALTVD
                     RTPLKLVGRFDRPVRSARLFSIDGAEGLPLKVAAAGNGTEVVIETGELKRHGIVILEP
                     A"
     gene            4685..>5434
                     /locus_tag="PKN80_06050"
     CDS             4685..>5434
                     /locus_tag="PKN80_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ35610.1"
                     /translation="MAKLAINGGPRAVPEGMLQPWPYITEADKQAVLKAMDDAMATLK
                     FTTNHPAVRGLEDGVARYVGSRFCLSANSGTAALHMAVAAAGLGPGDEVITSAYTFLA
                     SASCVLHHNAVPVFADIQPDTYNIDPASIEAHLTDRTRALLPVHIHGMAADMDEIMAI
                     ARRRGLAVIEDAAQAFGVDYRGRKAGTIGDLGAYSMESSKLLTSASEGGLLVTKIRQQ
                     PYAIVLFDEIEKAHPSVFDLFLQILDEGKLHD"
BASE COUNT          881 a         1900 c         1793 g          860 t
ORIGIN      
        1 ggcacgatgc actgggccgg cgggagccgg accggcggcc gcctgatcct ggccaggtaa
       61 gccggcggta aaggcccccg gcctgatatg gccgtgccga ctggatcgag cggagacgaa
      121 tgaaattaca cccccaatct ttttaccgga gaggacggca tggccaaaga gtttgagtgg
      181 cacaaggccg ggcatgaccg gcggctgaag gaaatcggct cccggatctg gttcgggacg
      241 caatattacc ggccgcccca gccgctgccc cgtttctggg agtcggattt caatctgatg
      301 aagaaggccg gcctcgaggt ggcccgggcc tggatttact ggcgctggca cgagcgggag
      361 ccggggcgct tccagttcga ggagctggac cgcctcttcg agctgggccg ccgccacggg
      421 gtcttcatcc acccgctcct ctttctggaa tcggccccgg agtggttcat ccgccagttc
      481 ccggagacct ggttcgttga ccaccagggg gtttcctact acccactcaa ccgcaagtcc
      541 acccagatcg gcggcatggc cgtctgcggc aaccacccgc tcacgaagga atggtcgggc
      601 cgcttcctga cggccctggt gaatcgctac cgggagcatc ccgccctgct ctgctgggat
      661 gtctggaacg agttgcagac caagtcggtc gaggggatct gcgcctgtca tcactgccgg
      721 gccgattacc ggtcccacct gcagcagcgg tacgcctccc tgggcgaact gaacgccgcc
      781 ctgaataccg gctacgggac ctgggaggat gtgctgccgc ctaacggcaa caacgattac
      841 gccgcctggt ttgcctggcg gcgctgggag gcgtccagcc tgagccgcca ggcgctctgg
      901 cgggcccgga ccatctgggc ggccgacccg cgccaggtgg tgatgatcca catgccgacc
      961 aagctttcgg tgcccggcca cggctgcggg gactggaatg actgcgcccc ttacctggac
     1021 ttcgccggca acagttccca catccagcgt tcgcaccggg gctactcgct gccggagcgc
     1081 ctttcggccc gcaacctgct ggcggccgag catgacatcg ccgtccagca gtcgcccaac
     1141 ttcgcctggg ccggcgaggt ttccagcgac accgccagtt acgatgcgcc gccgccgctc
     1201 cacccggacg aactggccta ctggacctgg aagccgatca gcctggggat gcggggggtc
     1261 ttctactggc agttccggcc cgaggcctac ggcccggagt cgcccgacct gggcctgctg
     1321 gaactggacg gccgggccag caaccgctgg gagcgggtgg gcgaaatcgt gaagctggtc
     1381 cgggagaatg aacccctctt ccgatcggcc cggccgccgg tgccgcaggt gggcatcctc
     1441 acctgcccgg agcagcatat ggcctgcgcc acgctttact ccaagaacag cggcggccag
     1501 caactcttcc agaagtcggt cctcaacctt tacgacgtct tccggagcgc cgggataccg
     1561 gccgccttcg tcgatccgga tcggatcggg tccgccatca agctgctgct ggtcccccgg
     1621 agtccggccg gccggccgga gttgaaggcc ggcctgctga agtttctcga agggggcggc
     1681 acggcggtgg tcgagggcgg tttcatgagc gccgacggcg ccagtttcat cctcgaggag
     1741 acggccccgg gcctgggcct ggaccgggtc ttcggcctgc gcgaggggcg gcccgagtcc
     1801 acccgctggc acggcctgcc gaccggcagc gaggagagcc gcctcagcaa gcaggaactg
     1861 gacgacgcct ggctggatgc cggcgtggac agggagacgg agcgttcccg tgagaagctg
     1921 gcgcccgagc tgcggatcag cctggcctgg ggcggccggg agtacccggt tacggccggc
     1981 ttcgaccggc ggccgctgcg ggcggccggg gccgagatca tcggccgttt cgccagcggc
     2041 gaagcggccc ttgccacgcg gcgggttggc aaggggcggc tgatttacat cgggaccgcc
     2101 gcttcctatg tcgaccggat cttcggcggc ggctacggcc gtttcctgcg ggcgctggcc
     2161 ggcgaactgg ccatccggcc gcccctgccg atcccgccgg cggccggggc cacctggcgg
     2221 gtgctcgagg ccggggcacg ccgccttctc ttctgtttca accacctgga aacgccggtc
     2281 gggttcgacc tgagccgctg cggcccggcc cggctcatct acggcccggg cgttgaactt
     2341 aaaggcggcc ggctgagcgt cgggccgctg gccaccgcgg tcctcgaact taaaaatcca
     2401 accaaggaga aatcaagatg aagacgacct atccggactg ggcccacggc atgcgcctgg
     2461 tcacctggag catttcggcg gccgacctgg acctgattac cccggccgag gccgaccggc
     2521 gggcgaagcg cctgcgccgg gagggcatca acgccgtggt ctccttcggc gcccatttcc
     2581 gctgggactg gatcaaggac ttcccgcgca tccgggaggt gacccggatc tcggtcgagg
     2641 cctgccaccg gcagggcatc aagtacatcg agcaccacag cgcctgcttc gtctcccgga
     2701 ttccggagcg gacgccggag gccttcgagt ggttcaaccg tcacctgccc aatcacaccg
     2761 tgcccgagcc gctggccctg gacgcggtgg tcgagtatcg gggcaagcgg ctcaacgact
     2821 ggtacgagat gaacctgaag gcgggcggcc cggagtactg gcagggttac aaggcctact
     2881 gcctctgccc gaaccatccc gaataccggc aggcctactt tgattacctg gaggatttcc
     2941 tgaagtacac cgacgccgac ggcatcatgt ccgacgacgt caccatgggc ctcaaccctt
     3001 acgcctgcgt ctgcggggag tgccgccgcc tcttcaagtc cgagaccggc tacgacctgc
     3061 cggagcgggc cgacgatccc ttctggtcgg agttcaaccg gcccgattac cgggcctggc
     3121 tcgatttccg gcggaactcg gtcctgcgcc actacgaaaa gatcgaccgg atgattcagg
     3181 gctggtccaa gccgctgctg cacacctcct gcgccaccac catcagttcg ccggtctcgg
     3241 ccgagaccta ccgccggcag gacgtcttca acaccacctt cgtcgaagtc ctcagcgcca
     3301 acccctacta ctacagctgg atgatgatct caccggaact gcgcatctac cgggcccggg
     3361 cccggggcgg cgccaacccc tccttcgcca tcttctaccc gcaggccaag gggggcgaac
     3421 agctcttcgg ctcggcctac ctgcgccagg aggagcactc ggtctggctc tcccccaaca
     3481 tcggccgcca cagtccgcag acggtcgggc gcgattcgga gtgggccctc tgggaggaga
     3541 agcacgatga tctcttccgg accgccggga cgctgaccga cctggccgtc ttcttctccg
     3601 acgccagccg gcgccgctac cggggccacc accacgagca ttacacctac gaattcaagg
     3661 gctggtgcga actcctgcac gaggcgggcc tcgattacga cgtggtgctg gaggaggact
     3721 tggccgccgg ccgcctcggc cagtaccgca ccctgctggc gcccaacgcc gcctgcctga
     3781 gcccggaact cagccgggcc atcgaagagt acgtgcgctc gggcggccgg ctgctcgcct
     3841 cgcacgaggc ctccctcttc aacttcgagg gggacccgct ggagaacttc cagctggccg
     3901 aatgcctggg tctggattac gaacggaccg tcttcgacga gcattacccg atccagggct
     3961 ggaacggcga cgacgccttc ctggccggcg tctccggact gattgcccac cgggcccccc
     4021 aggtccgggt ccggccgcgg ccgggcgccg aggtcctgct gacctccttc atcgacgccg
     4081 gcggccggaa gggagagatc atcccggccc tggtttccaa ccggttcggc cgggggctgg
     4141 cccgctactt cgccggcaag ccgggcatcc tctgctacca cccgggcatc cgcgcccgca
     4201 actgggacgg cgaggagccc taccggttcg tcaattatcg ggatccgctg ctggcccggg
     4261 tcttcctgaa cgcggtgcgg ctgacggccg ggagtttcag gctcgaactg ccgaacgcga
     4321 agccgggcgt cttctgccgg gggtaccggc tccgggacgg ccggctggcg gtccatatcc
     4381 tgaacgcggc cggcagcatc attccgcacc ggaccctggt ccagcccggc taccgggtca
     4441 agtacccggc cctgaccgtc gaccggaccc cgctgaagct ggtcggccgg ttcgaccggc
     4501 cggtccgctc cgcccgcctc ttctcgatcg acggggccga ggggctgccc cttaaggtgg
     4561 ccgcggccgg caacgggacc gaggtggtca tcgagaccgg cgagctgaaa cgccacggga
     4621 tcgtgatcct ggaaccggcc tgaggcgatc cgggaaagtt taagcatcaa gggaggcgta
     4681 acgcatggcg aaactggcga tcaacggagg tccccgggcc gtccccgagg ggatgctcca
     4741 gccctggccc tacatcaccg aggccgacaa gcaggcggtc ctcaaggcga tggacgatgc
     4801 catggccact ctcaagttca ccaccaacca cccggccgtc cggggcctgg aggatggagt
     4861 ggcccggtac gtgggcagcc gtttctgcct ttcggccaac agcggcaccg ccgccctgca
     4921 catggcggtg gccgccgccg gcctggggcc gggcgacgaa gtgatcacct ccgcctatac
     4981 cttcctggcc tcggcctcct gcgtcctgca ccacaacgcg gttccggtct ttgccgacat
     5041 ccagccggat acctacaaca ttgacccggc cagcatcgag gcgcacctca ccgaccggac
     5101 ccgggccctc ctgccggtcc acatccacgg gatggcggcc gacatggacg aaatcatggc
     5161 catcgcccgg cgccgcggct tggcggtcat cgaggatgcc gcccaggcct tcggggtgga
     5221 ttaccggggg cggaaggccg gcaccatcgg cgacctgggg gcctacagta tggagagttc
     5281 caagctgctg accagcgcca gcgagggcgg cctgctggtg accaagatcc gccagcagcc
     5341 ctatgcaatc gtgctgttcg acgagatcga gaaagcgcac ccctcggtgt tcgacctctt
     5401 cctgcagatc ctggacgagg gcaaactgca cgac
//
LOCUS       DAOQYN010000131         5426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_15367_length_5426_cov_3.905046, whole genome shotgun sequence.
ACCESSION   DAOQYN010000131 DAOQYN010000000
VERSION     DAOQYN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15367_length_5426_cov_3.905046"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PKN80_06055"
     CDS             complement(<1..961)
                     /locus_tag="PKN80_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35611.1"
                     /translation="MRLIGVDENGYGPLLGPLLVTAARARTDRHRPAPDLWEALEIGR
                     RPGPGPFICDSKLVFGAGTSRAERGEEMVLALLLARTGELADSADRFLELVLANAELD
                     LEFPCRAGGLRPVCWEENFKLELAGDRRRRIEQLARRFEASGRAAGLELEIPRAALFC
                     PRLFNERCGPAAKSNKSELVIRTGLSLSGRGRETELAAVLGKVGGRHYYARHLSENFP
                     GCRVETLQEEPGISAYQLRDGERLHRFTYLADGESRDFLVAAASLFGKYSREIFWRKV
                     LRFFAARSPEPLPPASGYRDGLTRRFVAATAGTRKRLGIPDDCF"
     gene            complement(966..2246)
                     /locus_tag="PKN80_06060"
     CDS             complement(966..2246)
                     /locus_tag="PKN80_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HNQ35612.1"
                     /translation="MLTVWGSVILLATVFVVVAMGKIEAAVISLLAAVVVLATGVLGQ
                     AEAFAAIDWNTIFLLVGMMMVVAVAKKTGLFQYLAIRSAKAARGNPLLILLFFPAITA
                     LISAFLDNVTTVLLIAPVTILIADSLELDPVPFLIAEAIASNIGGTATLIGDPPNIMI
                     GSAAGLGFNAFILNLTPVILVIQLAFSLLLYLLFRRRFHVNPELKAGIMEMDENRAIT
                     DPHLTRVSLTILGLILIGFVCHQQLGLEPATIALAGATLLLFLSQVPSLEIFQEVEWT
                     TIFFFIGLFIVVAAVDKQGVFAFLGHSILGLTEGHLLLTALLVLWISAFAAAFVGAVP
                     FVASMIPLLKAVTAQMPSHAIILWWALSLGACLGGNGTLTGAAANMVVADIARKNDRP
                     ISYLHFFRWGFPVMLLSLLISSLYIFLRCLYCPR"
     gene            complement(2288..2755)
                     /locus_tag="PKN80_06065"
     CDS             complement(2288..2755)
                     /locus_tag="PKN80_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ35613.1"
                     /translation="MQVKNIMDAGCVSVSEQASIKELIDCFRRVPRSTLPVVDSQGRL
                     VGIVSLHDLIRHFLPRYMDLLPSLQFLGESKTLEKKMLGQILDPAFALLFLVHDVMIK
                     KPVSVLETDSTFKAMALMIQHNYHHLPVTDRQGGFLGMVDQNRIILSFLNQAA"
     gene            2821..4227
                     /gene="cysS"
                     /locus_tag="PKN80_06070"
     CDS             2821..4227
                     /gene="cysS"
                     /locus_tag="PKN80_06070"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNQ35614.1"
                     /translation="MSLRFWNTMTRRLDEFQPLEPGRVGLYTCGPTVYDFAHLGNFRA
                     YIFEDLLRRYLEYSGFQVTQVMNITDIDDKTIRRSREEGLSLTDYTRRYEKAFYEDRD
                     ALRIKPAAFYPRATDHIPEMVELVRRLEAGGFAYRKDGSIYFSIEKFPGYGRLAGIEA
                     GALKAGVRVDSDEYEKEDVRDFVLWKESKPGEPVYETTLGPGRPGWHLECSAMSMKYL
                     GETFDIHTGGVDNIFPHHENEIAQSEAATGRPFARYFLHCAHLMVEGEKMSKSKGNFY
                     TLRDLLARGHDPVAVRALLLAVHYRSPLNFTFDGLAAAGRSVERIQGFYRRLDSGPAG
                     PGAGAAGRLEAAGKEFRAALDDDLNISAALAAVHNLVSALNPDLEAGRLSADERAAVR
                     RGLEDFDRVLALLPGDSGPGLTEAESALVAERQAARGRRDFARADQLRDELLRLGIVI
                     EDTPAGPRAVRRERPSGS"
     gene            4263..>5426
                     /gene="folP"
                     /locus_tag="PKN80_06075"
     CDS             4263..>5426
                     /gene="folP"
                     /locus_tag="PKN80_06075"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNQ35615.1"
                     /translation="MRIRPLIPRGAGLARELKRIGVDPAGVRIMAPKSDLLVLEVDDL
                     DYRAALILKQELLSRGAEAAISRRAYRLGGGSGPAILIGRRAEFEQLTVRLARQPFGL
                     KELAGRLRSFLESVAPLSWQLAGRRLYLDRPLVMGILNITPDSFSDGGRYQDPAAALE
                     QALRLEAEGADILDVGAESTRPGSRPISAEEERRRLDPVLKLLLPRLRLPVSVDTSKP
                     EVARAALDAGAAVINDVTAGAAAGMFKLAARSGAGLVLMHMQGRPETMQVNPSYRDAP
                     AEIYAFLAERLEAAAAAGVGQEQLVVDPGIGFGKSLEHNLLLLRRLGEFTGLGRPVLV
                     GPSRKGFIGALTGLPMEARVEGTLAAVLAAFQAGASIFRVHDVQPVKRALAVAG"
BASE COUNT          892 a         1876 c         1840 g          818 t
ORIGIN      
        1 agaaacaatc atccgggata cccagccgtt tgcgagtccc ggcggtcgcg gccacgaagc
       61 ggcgggtcag gccgtcccgg tatccgctgg ccggcggcag cggctccggg ctgcgggcgg
      121 cgaagaaccg gaggaccttc ctccagaaga tttcccggct gtacttgccg aagagcgagg
      181 cggccgcgac caggaagtcc cggctttcgc cgtcggccag gtaggtgaac cggtggagcc
      241 gttccccgtc ccggagctgg taggccgaga tgcccggttc ctcctggagc gtctccaccc
      301 ggcagcccgg aaagttttcg gagaggtggc gggcatagta atggcggccg cccaccttgc
      361 ccagcaccgc ggccagctcc gtctcccggc cgcggccgga gagggacagg ccggtccgga
      421 tcaccagttc cgatttgttc gatttggccg ccggcccgca gcgttcattg aagaggcgcg
      481 ggcagaagag ggccgcccgg ggtatctcca gctccaggcc cgccgcccgg ccggacgcct
      541 cgaagcggcg ggccagctgc tcgatccgcc gccgccggtc cccggccagt tccagcttga
      601 aattctcttc ccagcagacc ggccgcagtc cgccggcccg gcagggaaat tccaggtcca
      661 gttcggcgtt ggccagcacc agctccagga agcggtcggc cgagtccgcc agttcgccgg
      721 tccgggccag caggagggcc agcaccatct cctcgcccct ctccgcccgg cttgtcccgg
      781 ccccgaagac cagcttgctg tcgcagatga agggccccgg tccgggccgg cgcccgatct
      841 ccagcgcctc ccagaggtcg ggcgccgggc ggtgccggtc cgtccgggcc cgggcggcgg
      901 tcaccagcag cggccccagc agcggtccgt aaccgttttc gtcaacgccg atcagccgca
      961 tcgggtcagc gcgggcagta aaggcagcgc aggaagatgt agagggagga gatcagcagc
     1021 gagaggagca tgaccgggaa gccccaccgg aagaagtgca ggtaactgat cggccggtcg
     1081 ttcttgcggg cgatgtcggc caccaccatg ttggcggccg cgccggtcag cgtcccgttg
     1141 ccgcccaggc aggcgcccag cgaaagggcc caccagagga tgatcgcgtg ggacggcatc
     1201 tgggcggtga ccgccttcag cagcgggatc atcgaagcca cgaagggtac cgcgcccacg
     1261 aaggcggccg cgaaggccga gatccagagg accagcaggg cggtaagcag gaggtgcccc
     1321 tcggtcagtc ccagtatcga gtggcccagg aaggcgaaga ccccctgctt gtccaccgcg
     1381 gccaccacga tgaagaggcc gatgaagaag aagatggtcg tccactccac ctcctggaat
     1441 atctccaggc tcggcacctg gctgaggaag agcagcaggg tggcgccggc cagggcgatg
     1501 gtggccggtt ccaggcccag ctgctggtgg cagacgaagc cgatcaggat caggcccagg
     1561 atggtcaggg agaccctggt caggtgcgga tcggtgatgg cccggttctc atccatctcc
     1621 atgatgccgg ccttcaattc cgggttgacg tggaagcgcc gccggaagag caggtaaagc
     1681 aacagggaaa aggccagctg gatgaccagg atgaccgggg tcaggttgag gatgaaggcg
     1741 ttgaagccca ggccggcggc gctgccgatc atgatgttgg gcgggtcgcc gatcagggtg
     1801 gcggtgccgc cgatgttgga ggcgatcgcc tcggcgatca ggaacgggac cgggtccagt
     1861 tccagcgagt cggcgatcag gatggtcacc ggcgcgatca gcagcaccgt ggtgacgttg
     1921 tccaggaagg ccgagatcag ggcggtgatg gccgggaaga agagcaggat cagcagcggg
     1981 ttgccccggg ccgccttggc cgacctgatg gccaggtact ggaagaggcc ggtcttcttg
     2041 gccaccgcca ccaccatcat catccccacc agcaggaaga tggtgttcca gtcgatcgcg
     2101 gcgaaggcct cggcctggcc gagcaccccg gtcgccagga ccaccaccgc cgccagcagc
     2161 gagatgacgg ccgcctcgat cttgcccatg gcgaccacca cgaagacggt ggccagcaaa
     2221 atgacgcttc cccagacggt caacattttt tggcgcggcc gccccccggc ggaaacctcc
     2281 gcggggatca ggccgcctgg tttaaaaaag aaaggatgat gcggttctgg tccaccatgc
     2341 ccaggaagcc gccctgccgg tcggtcaccg ggaggtggtg gtaattgtgc tgtatcatca
     2401 gggccatcgc cttgaaggtc gagtcggtct ccagcaccga gaccggcttc ttgatcatca
     2461 cgtcgtgcac caggaagagg agcgcgaagg ccgggtccag tatctggccc agcatcttct
     2521 tctcgagcgt cttcgattcg cccaggaact ggagcgaggg cagcaggtcc atgtaccggg
     2581 gcaggaaatg ccggataagg tcgtgcaggg agacgatgcc gaccaggcgt ccctggctgt
     2641 cgaccaccgg cagggtggaa cgcgggacgc gccggaagca gtcgatcagc tccttgatgc
     2701 tcgcctgttc ggagaccgag acgcagccgg catccattat gttttttacc tgcatttggt
     2761 gttcccgtcg ctattatacc acaaaataat ccggggccgc ggctgtgcta taatatccgc
     2821 atgtcactgc gattctggaa caccatgacc aggcggctgg acgagttcca gccgctcgag
     2881 ccgggccggg tcggtctcta cacctgcggc ccgaccgtct acgatttcgc ccacctgggc
     2941 aacttccggg cctacatctt cgaggacctc ctgcgccgct acctggagta ctcgggcttc
     3001 caggtcaccc aggtgatgaa catcaccgac atcgacgaca agaccatccg ccgcagccgc
     3061 gaggagggtc tttcgcttac cgactacacc cgccgctacg aaaaggcctt ctacgaggac
     3121 cgcgacgccc tccgcatcaa gccggccgcc ttttatcccc gggccaccga ccacatcccg
     3181 gagatggtcg agctggtccg gcgcctggag gccggcggct tcgcctaccg gaaggacggc
     3241 tccatctatt tttccatcga gaagttcccc ggttacggcc ggctggccgg gatcgaggcc
     3301 ggcgccctca aggccggggt gcgcgtcgac agcgacgagt acgagaagga ggacgtgcgc
     3361 gatttcgtgc tctggaagga gagcaagccg ggtgagccgg tctacgaaac gacgctgggt
     3421 ccgggccggc cgggctggca cctggaatgc tcggccatga gcatgaagta cctgggcgag
     3481 accttcgaca tccataccgg cggcgtcgac aacatcttcc cccaccacga gaacgagatc
     3541 gcccagagcg aggcggccac cggccgtccc ttcgcccgtt acttcctcca ctgcgcccac
     3601 ctgatggtcg agggcgagaa gatgtccaag agcaagggca acttctacac cctgcgcgac
     3661 ctgctggccc gcggccacga cccggtcgcg gtgcgggccc tgctgttggc ggtccactac
     3721 cgcagcccgc tcaacttcac cttcgacggc ctggccgccg ctggccgttc cgtggagcgc
     3781 atccagggct tctaccgccg gctcgattcc ggtccggccg gcccgggtgc cggcgccgcc
     3841 ggccgcctgg aggccgccgg gaaggagttc cgggccgccc tcgacgatga cctgaacatc
     3901 tcggccgccc tggccgccgt ccacaacctg gtcagcgccc tcaatccgga cctggaagcc
     3961 ggccggctga gcgccgatga acgggccgcg gtccggcgcg gcctggagga tttcgaccgg
     4021 gtgctggcct tgctgccggg tgattccggc ccgggcctca ccgaggctga gtcggccctg
     4081 gtggccgagc ggcaggcggc ccgcggccgc cgggacttcg cccgcgccga ccagctccgc
     4141 gatgaattgc tccgcctggg catcgtcatc gaggacactc cggccggtcc ccgggcggtc
     4201 cgccgggagc ggccgtccgg ttcgtgaagc cggccgggtc cggccatgaa aaccgatacc
     4261 caatgcgaat cagaccgttg atcccccggg gcgccggcct ggcccgggaa ctgaagcgga
     4321 tcggggttga tccggccggg gtccgcatca tggcccccaa gtccgacctc ctggtgctgg
     4381 aggtggacga cctcgactac cgggccgcgc tcatcctcaa gcaggaactc ctttcccggg
     4441 gcgccgaggc ggccatcagc cgccgcgcct accggctggg cggcggttcc ggcccagcca
     4501 tcctcatcgg ccgccgcgcc gaattcgaac agctgaccgt ccggctggcc cgccagccct
     4561 tcggcctcaa ggaactggcc ggccgcctcc gctcctttct ggaatcggtg gcgccccttt
     4621 cctggcagct ggccggccgc cgcctctacc tcgaccggcc gctggtcatg ggcatcctta
     4681 acatcacgcc cgattccttc tccgacggcg gccgctacca ggacccggcg gccgcgctcg
     4741 agcaggcgct ccgcctcgag gcggaagggg ccgatattct cgacgtcggc gccgagtcca
     4801 cccggcccgg cagcaggccg atcagcgccg aggaggagcg gcggcgcctg gacccggtcc
     4861 tcaagctgct gcttccgcgc ctgcgcctgc cggtctcggt cgacacctcc aagcccgagg
     4921 tcgcccgggc cgccctcgac gccggcgcgg ccgtcatcaa cgacgtcacc gccggagccg
     4981 cggccggcat gttcaaactg gccgcccgga gcggcgccgg cctggtcctg atgcacatgc
     5041 aggggcggcc ggagaccatg caggtcaacc cttcctaccg ggatgccccg gccgagatct
     5101 acgccttcct ggccgaacgc ctcgaggcgg ccgccgccgc cggcgtcggc caggaacagc
     5161 tggtggtcga tcccggcatc ggcttcggca agagcctgga gcacaacctg ctcctgctcc
     5221 ggcggctcgg cgagttcacc ggcctgggcc ggccggtcct ggtcggcccc tcgcgcaagg
     5281 gcttcatcgg cgccctgacc ggcctgccga tggaggcgcg ggtcgagggg accctggcgg
     5341 cggtgctggc cgcctttcag gccggcgcct ccatctttcg ggtgcatgat gttcagccgg
     5401 tcaagcgggc cctggccgtg gccggg
//
LOCUS       DAOQYN010000132         5306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_15823_length_5306_cov_3.893925, whole genome shotgun sequence.
ACCESSION   DAOQYN010000132 DAOQYN010000000
VERSION     DAOQYN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15823_length_5306_cov_3.893925"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..417)
                     /locus_tag="PKN80_06080"
     CDS             complement(52..417)
                     /locus_tag="PKN80_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNQ35616.1"
                     /translation="MEDRIVNELVIMRLQPGENLMERLVEAGRKYGVRLGVVVSGLGT
                     TRKVRLGYFRSPGNYDTRTFEGVHELLALSGGLRLEGNELKPHLHATVSGPDLQAWGG
                     HLLELEAAVMVEVVLRKIE"
     gene            complement(417..737)
                     /locus_tag="PKN80_06085"
     CDS             complement(417..737)
                     /locus_tag="PKN80_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35617.1"
                     /translation="MKRSLTTAARRRVEGIHRLSRRRARREGRKHLACLLEMLSEHAV
                     EIRDLQGRGDRHYLVETGDLALLCLEIMLEGRRDPEAILEECFGRFERKLVSPASGGA
                     PRSG"
     gene            complement(734..1192)
                     /locus_tag="PKN80_06090"
     CDS             complement(734..1192)
                     /locus_tag="PKN80_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNQ35618.1"
                     /translation="MKYGAGEIGRVFVIRLEDGDRLPESIEAFAREKGVRHGFCLLVG
                     GLKAGNLVVGPADPSARPVDPMLLPFNAVHEVSGVGTLFPDETGRPRLHLHAAIGRGD
                     RSAVGCLRPGVEVWQLGEVILVEILRSRARRLKDPETGFEMLEPGGEKVP"
     gene            complement(1204..2478)
                     /locus_tag="PKN80_06095"
     CDS             complement(1204..2478)
                     /locus_tag="PKN80_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35619.1"
                     /translation="MTSRERVIKAVNHQEADRVPLDLGASPVTGMHVSTVYRLRQALE
                     LDPPGTPVKVVEPLQMLGEIGDDLREALGVDTVGLQVPVTNFGFRLEGWKEWRLFDAT
                     PVLVPAGFNTEPEPNGDILLYPHDDRSVPASGRMPKDGWYFDAIVRQPPLDENNLKVE
                     DNLEEFGPASGENLAWLKAEAERLYTRTDRAILANFGGTSFGDIARVPGTALKHPRGI
                     RDMAEWYMSLASRPEHLYRIFEKQCEIGLANLEKYYQAVGDRVTAVMISGTDFGAQHG
                     PFISPATYRKLFQPFHRQVNDWVHRHTPWKIFIHTCGSVRDLLPDLCDAGFDILNPVQ
                     TSAARMDPAELKREFGSRLVFWGGGLDTQRVLPFGSPREVTDQVRERLRTFGAGGGFV
                     FNTVHNVQARVPVENVLALYQAVRRHGVYPLD"
     gene            complement(2503..2919)
                     /locus_tag="PKN80_06100"
     CDS             complement(2503..2919)
                     /locus_tag="PKN80_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNQ35620.1"
                     /translation="MKSVTEHLSFCTKQRREFVNITERIQTLVVRSGIREGLCLVNSM
                     HITSSVFINDEEPGLKRDFQQWLDRLVPEDPSLYYHSRTGEDNGAAHLRRTIMGREVV
                     IGVTGGQLDLGPWEQIFYGEFDGQRPKRVLVKIIGA"
     gene            3033..3743
                     /locus_tag="PKN80_06105"
     CDS             3033..3743
                     /locus_tag="PKN80_06105"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNQ35621.1"
                     /translation="MKLNLYLARAGVASRRRSAELVKSGRAAVDGKTVLQPSFEVEPG
                     QAVTLDGRPLQPETRRYLALNKPAGYTCTLRDPFASSKVTALVPAELGRLYPVGRLDR
                     ESTGLVILTNDGDLAERLTHPRYGVEKEYRVVVAPPVEPGDLARLRSGVFDEGERLRA
                     AGARLTGPAAGGKSELSLILKEGRKREIRRLFAQLGRRVIELERVRIGTVRLGRLPAG
                     RWRSLTPAELAGLGAGRE"
     gene            complement(3699..4628)
                     /locus_tag="PKN80_06110"
     CDS             complement(3699..4628)
                     /locus_tag="PKN80_06110"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNQ35622.1"
                     /translation="MISRFPDFTPLNLELQAELKAYLKANPPLGSEYTFTNLFGWQRS
                     ADYQAARFGDGFLLRRSHRSGRETLLEPLVTRGGLEALEAGFDYLAGRGRMAEAERVT
                     ESFLERFPAVAEKFRVVEDRDNHDYLYLVRELIDLAGEKFHDKQNLYRQFVRKYRFSY
                     RPMTGADIQAGLDFVDHWCRKRECDKYEGLNQEKRAVRRMLKNFETLGLRGGLLEIEG
                     NPVALTLGEPLNPETYVIHVEKADSAYTGVYQAINREFLKEAAAGFRFVNREQDMGLL
                     GLRQAKLSYNPVRLVRKYRLFPAGAQAGQLGGS"
     gene            complement(4710..5249)
                     /gene="lepB"
                     /locus_tag="PKN80_06115"
     CDS             complement(4710..5249)
                     /gene="lepB"
                     /locus_tag="PKN80_06115"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNQ35623.1"
                     /translation="MSRRFVIIDAVVSVLIIFAAYFFFSVKQGGFFYIDSESMEPTLQ
                     PGDRLVLVKAGSLERGQIVVFRHESRKVEFYVKRLVGLPGDQVAVRGGRLYLNGQAQE
                     EPYLKERRIVYNYGPIQVPEAEYFVLGDNRNNSEDSSVFGCVPAGDIRGRVLGIYWPW
                     HRRLRLALSGSAAVIAASA"
BASE COUNT          929 a         1778 c         1671 g          928 t
ORIGIN      
        1 ccgccagtcg aacttcttct cgtccatcgt ttccccctgt cgtcttaacg gttactcgat
       61 cttccggaga acgacctcga ccatgaccgc cgcctccagt tccagcaggt ggccgcccca
      121 ggcctggagg tcggggccgg agaccgtggc gtgcaggtgc ggcttgagtt cgttcccctc
      181 aagacgcagg ccgccggaaa gggccagcag ttcgtggacg ccctcgaagg tgcgggtgtc
      241 gtaattgccg gggctgcgga agtagccgag ccgcaccttt cgggtggtcc ccagcccgga
      301 gactaccacg cccagcctga ccccgtactt gcggcccgct tcgaccagcc gctccatcag
      361 gttctcgccc ggctgaagcc gcatgatgac cagctcgttg acgattcgat cctccattat
      421 ccactccttg gcgccccgcc ggaggccggc gaaaccagct ttctttcaaa acggccgaaa
      481 cactcttcca ggatggcctc gggatcccgg cgcccctcca gcatgatttc caggcagagc
      541 aacgccaggt cgccggtctc caccaggtag tggcggtcgc cccgcccctg gagatcccgg
      601 atctcgaccg cgtgctcgga aagcatctcc agcaggcagg ccaggtgctt ccgcccttcc
      661 cggcgggcgc ggcggcgtga caggcggtgg atgccctcca cccgccgccg ggccgccgtg
      721 gtcagggagc gtttcacgga actttttctc cgcccggttc cagcatctcg aacccggttt
      781 cggggtcctt caaacgccgc gcccggctcc tcaggatctc gaccaggatc acctcgccca
      841 gctgccagac ctcgacgccg ggacggaggc agccgaccgc gctccggtcg ccccgtccga
      901 tggccgcgtg caggtgcagg cggggccggc cggtttcgtc cgggaagagg gtgccgactc
      961 cggagacctc gtgaaccgcg ttgaacggca ggagcatcgg gtcgaccggc cgggcggacg
     1021 ggtcggccgg gccgaccacc aggtttcccg ccttcaagcc tccaacgagc aagcaaaatc
     1081 catggcggac gcccttctcc cgagcaaagg cttcgattga ttccgggagg cggtcgccat
     1141 cctccaggcg gatgacgaag acccgtccga tttcaccggc gccgtatttc atgccaaccc
     1201 cattcaatca agcggataaa cgccgtggcg gcggaccgcc tggtaaaggg ccaggacatt
     1261 ctcgaccggc acccgggcct ggacgttgtg gacggtgttg aagacgaaac cgccgccggc
     1321 gccgaaggtg cgcagacgtt ccctgacctg gtcggtcacc tcccgcgggg aaccgaacgg
     1381 aagcacccgc tgggtgtcga ggccgccgcc ccagaagacc agccggcttc cgaattcccg
     1441 tttcaactcg gccggatcca tgcgggccgc cgaggtctgg accgggttca ggatatcgaa
     1501 acccgcgtcg cagaggtcgg gcagcaggtc gcgcaccgaa ccgcaggtat gaatgaatat
     1561 cttccagggt gtgtggcgat gaacccagtc gttgacctgg cggtgaaagg gctggaagag
     1621 cttccggtag gtggccgggg agatgaaggg tccgtgctgg gcgccgaaat cggtgccgct
     1681 gatcatgacc gccgtcaccc ggtcgccgac cgcctggtaa tacttttcaa ggttagccag
     1741 tcctatttcg cactgtttct cgaagatccg gtaaaggtgt tccggccggc tggccaggct
     1801 catgtaccac tcggccatgt cgcggatgcc ccggggatgc ttcaaggcgg tcccgggtac
     1861 ccgggcgatg tcgccgaagg aggtgccgcc gaagttggcc aggatggccc ggtcggtccg
     1921 ggtgtaaaga cgttcggcct cggccttcag ccaggccagg ttctccccgg aggccggacc
     1981 gaattcctcg aggttgtctt cgaccttcag gttgttctcg tccagcggcg gctggcggac
     2041 gatggcgtcg aagtaccagc cgtccttggg catccggccg ctggccggca ccgagcggtc
     2101 gtcgtgcgga taaagcagga tgtcgccgtt gggctccggc tcggtgttga agccggccgg
     2161 caccagcacc ggggtggcgt cgaagaggcg ccattccttc cagccctcga gacgaaaacc
     2221 gaagttggtc accggaacct ggaggccgac cgtgtccact cccagggcct cccgcaggtc
     2281 gtccccgatc tcgcccagca tctggagcgg ttcgaccacc ttgaccggcg tgccgggcgg
     2341 atccagttcc agggcctggc ggagccggta gacggtgctc acgtgcatcc cggttaccgg
     2401 gctggcgccc aggtccagcg gcacccggtc cgcttcctgg tggttgacgg ccttgatcac
     2461 cctttcgcgc gaagtcactt cgattccccc tccgatttct tctcaggcgc cgatgatctt
     2521 gaccagcacc cgcttgggcc gctggccgtc gaactccccg taaaaaatct gctcccaggg
     2581 ccccaggtcc agctggcccc cggtgacccc gataacgacc tcgcgcccca tgatggtccg
     2641 cctcaggtgg gccgcgccgt tatcctcgcc ggtccggctg tggtaataga gcgacgggtc
     2701 ctcgggaacc agccgatcca gccactgctg gaaatcgcgc ttcagcccgg gttcctcgtc
     2761 attgatgaaa acgctggagg tgatgtgcat ggagtttacc aggcagagcc cctccctgat
     2821 gccgcttctt accaccagcg tctggatgcg ctcggttata ttcacgaact cccggcgctg
     2881 cttggtgcag aaggaaagat gttcggtcac cgatttcact ttgtctcccg tcctggcccg
     2941 gcttccaaaa ggatccgccc ccgggttata attatacaat agaatatcgc ctggaccctt
     3001 attcagtcaa ggaaaaaacg actcaaccgc ccatgaaatt gaatctttac ctggcccgcg
     3061 ccggggtcgc ctcgcggcgc cggagcgccg agctggtcaa atccggccgg gccgccgtcg
     3121 acgggaaaac ggtccttcaa ccctcgttcg aagtcgaacc ggggcaggcg gtaacactcg
     3181 acgggcggcc gctgcagccg gaaacccgcc gttacctcgc cctcaacaaa ccggctggat
     3241 acacctgcac cctccgcgac cccttcgcca gctcaaaggt gaccgccctg gtgccggccg
     3301 agctgggccg cctctacccg gtcgggcggc tggaccggga gtcgaccggc ctggtcattc
     3361 ttaccaacga cggcgacctg gccgaacgcc tcacccatcc acgctacggg gtcgaaaaag
     3421 agtaccgggt ggtggtggcg ccgccggtcg aaccgggaga tctggcccgg ttgcgttccg
     3481 gcgtcttcga cgagggggaa cggctgcggg ccgccggcgc ccggctgacc gggcccgccg
     3541 ccggcggcaa gtcggagctg agcctgatcc tcaaggaggg acggaaacgg gaaatccggc
     3601 gcctgttcgc ccagctcggc cggcgggtca tcgagctgga acgcgtccgc atcgggacag
     3661 tccgcctggg ccggctgccg gccggccgct ggcgcagttt aactcccgcc gagctggccg
     3721 gcctgggcgc cggccgggaa tagccggtac tttctgacca ggcggaccgg gttgtaggaa
     3781 agcttggcct ggcgcagtcc gagcaggccc atatcctgct cccggttcac gaaccggaaa
     3841 ccggccgcgg cctcctttag aaactcacgg ttgatggcct ggtagacacc ggtataggcg
     3901 ctgtcggcct tctcgacgtg aatgacgtag gtctccggat tgagcggctc gcccagggtc
     3961 agggcgaccg ggtttccctc gatctcgagc aggccgccgc gcaatccgag cgtctcgaaa
     4021 ttcttcagca tccttcgcac cgcccgcttc tcctggttca aaccctcgta cttgtcgcac
     4081 tcccgtttcc ggcaccagtg gtcaacgaaa tcaaggccgg cctgaatgtc ggcgccggtc
     4141 atggggcggt aggaaaaccg gtacttgcgg acaaactggc ggtaaaggtt ctgcttgtcg
     4201 tgaaacttct ccccggccag gtcgatcaac tcccggacca ggtaaagata atcatggtta
     4261 tcccggtcct cgaccacccg gaacttttcc gcgaccgcgg ggaagcgttc caggaaagat
     4321 tcggtgactc tttccgcttc ggccatccgg ccgcggccgg ccaggtaatc gaaaccggcc
     4381 tcgagggcct cgaggccgcc cctggtgacc agcggctcca gcagagtctc ccggccggaa
     4441 cgatgactgc gccggagcag gaaaccgtct ccaaaacggg ccgcctggta atcggccgac
     4501 cgctgccagc cgaagagatt ggtaaaggtg tactccgatc cgagcggcgg attcgccttc
     4561 aggtaggcct tcaattcagc ctgcaattca agattgagcg gagtgaaatc cgggaacctg
     4621 gagatcatgt tggcggtctt tctcccgtgt gaaacgtgaa aattgggggg cgttgaaaag
     4681 agggaccggc ttcccgcagc cggtcccggt caagccgaag ccgcaatgac ggcggccgat
     4741 ccggacaggg ccagccgcag ccgccggtgc cagggccagt agatacccag cacccggccc
     4801 cggatatccc cggccggcac gcagccgaag acgctgctgt cctcgctgtt gttccggttg
     4861 tcgcccaaca cgaagtactc tgcttccggg acctggatcg ggccgtagtt gtaaacgatc
     4921 cggcgttctt taagatacgg ctcttcctgg gcctggccgt tgagatagag gcggccgccc
     4981 cggacggcga cctggtcacc cgggagaccg acgagccgct tcacgtaaaa ttcgaccttc
     5041 cgggattcgt gtctgaagac cacgatctgc ccccgttcca ggctgccggc cttgaccagc
     5101 accaaccggt cgccgggctg gagggtgggc tccatcgatt cggagtcaat gtaaaagaag
     5161 cccccctgct tgacgctgaa gaaaaaatag gcggcaaaga tgatcaggac cgaaacgacg
     5221 gcgtcaatga tgacgaacct ccggctcatc cacccccctc gggcgccagg ctgaccgaac
     5281 ccttccgggc gtcgtagacc aggcgg
//
LOCUS       DAOQYN010000133         5286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_15892_length_5286_cov_5.415599, whole genome shotgun sequence.
ACCESSION   DAOQYN010000133 DAOQYN010000000
VERSION     DAOQYN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15892_length_5286_cov_5.415599"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PKN80_06120"
     CDS             complement(<1..332)
                     /locus_tag="PKN80_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35624.1"
                     /translation="MDNPRDIRVTGVRTWLEHYDFRTPLKFGGVVMYKTTILNAEIQV
                     ETRDGRRGAGLGSMILGNTWAWPSRVYGFQETMSLMEELGGRLASLIGASRDFGHPVE
                     LAAGLEES"
     gene            complement(361..3078)
                     /locus_tag="PKN80_06125"
     CDS             complement(361..3078)
                     /locus_tag="PKN80_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35625.1"
                     /translation="MSLRLFLPIALILFSLACLRPRPAAAHWEVQADDLPAWGQRGRL
                     TWAYHDSNPTTPEMARWPLVTHLHGPRPVTGAIVNERVRQGIPINLRLEAIMFFGTDS
                     IVRRQLDVYKGNRYRVDHDWYWQFNWFSRDPKFREAATVLRDGRLMVEYLGNNLTERE
                     MGNPLSPMLLRMREEQSRAILGRHDPASPANPVYPQFPYTPENDDVSFGKKGAPYAYY
                     PSFGYISSVWYDNPQLWADASEFSQRVWKGHFKEKFGLEIADPASHPSELVRREWRRF
                     WADAYGRYFEQYRNFHQENIRNSAPEIIPLTTALNGRRVCPVMLNASKLSHIWGANQL
                     YIFNRYRASDYPGVQCEFEERFTGGRHALPIKFGMASMHGRPTGKHGTNARAEGEALA
                     LNGNHSYVGLKAGTRAFEYADFQYNNGFLLENAQPANRIAILYNIRSGLVASNLIDAF
                     NLGRQLDELGLPYDVLIEDDLSDARTALVAGYAAVLVPGGEFNAGEVAGFQRYLAGGG
                     RLVLIGDAILEPPQYRLISPEESGADPWRPDQPLAGALGAAGFGEARVKSGRGELAVF
                     DDQVVSNAKLNAALAPCLASTFRLPESRGGLVMANVLRQPRQGDALVIGLVNYGGQVQ
                     KEVKIALPSDLRAADCAVISPDGYAGRLKVSRGVITVPELYQYSAVVLGSRAVVEKAL
                     ASVAPKLEGLMKLREPLKPAAGPKDSPSEAQLRPEAVDPGRRLARLRHGSNESNLAVC
                     LDAVAPRRVKAGAPVPVNLKMILPGTHGTAGTTLEYWRLQAVNIETGRPVLSQPLGAP
                     APIEGFPVYLVRDGKREAMPYRAAELAGQELAAEVFFSEPGRYQLFVDFLYNVGGPDF
                     AFTQGDAGPRVEPAFPGDRPAAGWGYPGRPLKKHYVNLKLPRMVVEVSR"
     gene            3244..4848
                     /gene="cimA"
                     /locus_tag="PKN80_06130"
     CDS             3244..4848
                     /gene="cimA"
                     /locus_tag="PKN80_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNQ35626.1"
                     /translation="MPKQDKEAGALQKIEVYDTTLRDGSQGEQVSFSLEDKLKLTRKL
                     DEFGFDFIEGGWPGSNAKDEEFFREVRKLDLARARVAAFGSTRRNGVAPAQDENLNKL
                     LSAETEIITLFGKSWDLHVRNVLRISLEENLDLISDSIDFLRRRGRRVFFDAEHFFDG
                     YRENPAYALKCLAAADQGGAERLVLADTNGGSLPDQVRAAMAEVKKITRRPLGIHTHN
                     DSGLAVANSITAVAEGAVQVQGTLNGLGERCGNADLLVIVPILQVKMNRPCLDPEKLA
                     RLTTLAHYTYEVANLTPPNNQPFVGASAFAHKGGIHVDAIRKEPRSYEHLDPALVGNE
                     RRILVSELSGGATILFKAERYGINKKGPETRRILAELARLEKAGYQFEAAEGSFQLLV
                     AEVLGKRPVFFQVQEFRVIVERKNGLIVSEASVKVKVGDRLEHTVAEGDGPVNALDAS
                     LRKALLSFYPSLADLRLTDYKVRVLNPEAATAAKVRVLIESADREETWGTVGLSENII
                     EASLQALADSIDYKLFKESAVSRHRP"
     gene            4962..>5286
                     /locus_tag="PKN80_06135"
     CDS             4962..>5286
                     /locus_tag="PKN80_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35627.1"
                     /translation="MKKWMLWLVLVAVAAFMAGCACKVSPVGPRAIVIDQPAAVVEKP
                     VVVEKTVERIIEKPVEKVVTVEKIVEKPVEKIVYVDKPVTVETKAYPGIRDVFFPYDS
                     AELTPL"
BASE COUNT          947 a         1768 c         1706 g          865 t
ORIGIN      
        1 caggactcct ccaggccggc ggccagttcc accgggtggc cgaaatcccg gctggccccg
       61 atgagggagg ccaggcgccc gcccagctcc tccatcaggg acatggtctc ctggaagccg
      121 taaacccggc tcggccaggc ccaggtgttg cccaggatca tcgagcccag gccggcgccg
      181 cggcggccgt cccgggtctc aacctggatc tcggcgttga gtatcgtggt cttatacatc
      241 accaccccgc cgaacttgag cggcgtccga aaatcgtagt gctccagcca ggtgcggacg
      301 ccggtcaccc tgatgtcacg cggattatcc ataagtcgcc tcttctccag tcttgtgggg
      361 ttatctggaa acctcgacca ccatccgggg cagtttcagg tttacgtagt gcttcttgag
      421 cggccgcccc gggtagcccc agccggcggc cggccggtcg cccgggaagg ccggctccac
      481 ccgcggcccg gcatcgcctt gggtgaaggc gaaatccgga ccgccgacgt tgtagagaaa
      541 atccacgaag agctggtagc ggcccggttc cgaaaagaag acctcggccg ccagctcctg
      601 gccggccagc tccgccgccc ggtagggcat cgcctcccgc ttcccgtccc ggaccaggta
      661 gaccgggaaa ccctcgatcg gcgccggggc ccccagcggc tggctgagga ccggccggcc
      721 ggtctcgatg ttcaccgcct ggaggcgcca gtattccaga gtggtgccgg ccgtcccgtg
      781 ggtgccgggc aggatcatct tcaagttcac cggcaccggc gccccggcct tcacccggcg
      841 cggggccacc gcgtccaggc agacggccag gttgctctcg ttgctgccgt ggcgcaggcg
      901 ggccaggcgc cgcccggggt ccaccgcctc cggccgcaac tgggcctcgc tgggcgaatc
      961 cttgggtccg gccgccggct tgagcggctc gcgcagtttc atcagccctt ccagcttcgg
     1021 ggccacgctc gccagggcct tctccaccac cgcccgcgaa ccgagcacca ccgcgctgta
     1081 ctggtagagt tccggcaccg tgatcacccc cctcgaaacc ttgaggcggc cggcgtaccc
     1141 gtcgggcgaa atcacggcgc agtcggccgc ccgcaggtcc gaaggcaggg cgattttaac
     1201 ctccttctga acctggccgc cgtaattgac cagaccgatg accagggcgt ccccctggcg
     1261 cggctggcgc agaacgttgg ccatcaccag cccgccgcgg ctctccggaa ggcggaaggt
     1321 actggccagg cagggcgcca gcgccgcgtt caatttcgcg ttggacacca cctggtcgtc
     1381 gaagaccgcc agctcgcccc ggccggactt gacccgggcc tctccgaacc cggccgcgcc
     1441 cagtgcgccg gccagcggct ggtccggccg ccaggggtcg gccccggatt cctccggact
     1501 tattaaccgg tattgtggcg gctccaggat cgcgtcaccg atcagcacca gccggccgcc
     1561 gccggccagg taccgctgga aaccggccac ctctccggca ttgaactcgc cgccgggcac
     1621 cagcaccgcc gcgtaaccgg ccaccagggc ggtccgcgcg tcggaaaggt catcctcgat
     1681 caggacgtcg tagggcaggc ccagttcgtc cagctgccgc cccaggttaa aggcgtcgat
     1741 caggttgctg gccaccaggc cgctgcggat gttgtaaagg atcgcgatcc ggttggccgg
     1801 ctgggcattc tcgagcagga acccgttgtt gtactggaaa tcggcgtact cgaaggcccg
     1861 ggtcccggcc ttcaggccga cgtaggagtg gttcccgttg agggccagcg cctcgccctc
     1921 ggcccgggcg ttggtgccgt gcttgccggt cggccggccg tgcatcgagg ccatgccgaa
     1981 cttgatcgga agcgcgtggc ggccgccggt gaagcgctcc tcgaattcgc actgcacccc
     2041 gggatagtcc gaggcccggt aacggttgaa gatgtaaagc tggttggccc cccagatgtg
     2101 ggacagtttg ctggcgttga gcatcaccgg gcagacgcgc cggccgttga gggcggtggt
     2161 caggggaata atctcaggcg ccgagttcct gatgttctcc tggtggaaat ttcgatactg
     2221 ctcaaagtag cggccgtagg cgtcggccca gaagcggcgc cactcgcgcc gcaccaactc
     2281 gctcgggtgg ctggccggat cggcgatctc gaggccgaac ttctccttga agtggccctt
     2341 ccagacccgc tgggaaaact cggaagcatc ggcccagagt tgcgggttgt cgtaccagac
     2401 cgagcttatg tagccgaagg acgggtagta cgcgtagggg gcccccttct tgccgaagct
     2461 gacgtcgtca ttctccggcg tgtaggggaa ctgcggatag acgggatttg ccgggctggc
     2521 cgggtcgtgg cggcccagga tcgcccgcga ctgctcttcc cgcatcctca gcagcatcgg
     2581 gctcaaggga ttgcccatct cgcgctcggt caggttgttg ccgaggtact cgaccatcag
     2641 ccggccgtcg cgcaggaccg tggccgcctc ccggaacttc gggtcgcggc tgaaccagtt
     2701 gaattgccag taccagtcat gatccacccg gtaacggttg cccttgtaga catcaagctg
     2761 gcgcctgacg atcgagtcgg tgccgaagaa catgatcgct tccaggcgca ggttgatcgg
     2821 gatcccctgc cgcacccgct cgttgacgat ggcgccggta accggccggg gaccgtgcag
     2881 gtgggtcacc agcggccagc gggccatctc cggcgtggtc ggattgctgt cgtggtaggc
     2941 ccaggtgagc cggccccgct gcccccaggc cggcaggtcg tcggcctgca cctcccagtg
     3001 ggcggccgcc ggccgcggcc gcaggcaggc aagggaaaac aggatcagcg cgatcggcaa
     3061 aaataacctc aggctcattt tttatcctct tcctcgagat cccggcgtga aaaagcggga
     3121 aggactttat taatgttagc atagcaacaa ggccgtccgg ttgtcaaaaa aagccgcgcc
     3181 ggcgcccgga aacacgcgtc cggctttgac atgacgcctc tgatcgggat aaactatccc
     3241 atcatgccaa aacaggataa agaggccggg gccctgcaaa aaatcgaggt ttacgacacc
     3301 accctgcggg acggcagcca gggggagcag gtctccttct ccctcgagga caagctcaag
     3361 ctcacccgca agctggacga gttcggcttc gatttcatcg agggcggctg gcccggcagc
     3421 aacgccaagg acgaggaatt cttccgggag gtccggaaac tcgacctcgc ccgggccagg
     3481 gtggccgcct tcggcagcac ccgccggaac ggcgtggcgc cggcccagga tgaaaacctc
     3541 aacaagctgc tttcggccga gaccgaaatc atcaccctct tcggcaagtc ctgggacctg
     3601 catgtccgga acgtgctccg gatctccctg gaggagaacc tcgacctgat cagcgactcg
     3661 atcgatttcc tcaggcgccg gggacggcgg gtcttcttcg acgccgaaca cttcttcgac
     3721 ggctaccggg aaaatcccgc ctatgccctg aaatgcctgg ccgcggccga ccagggcggc
     3781 gccgaacgcc tggtgctggc cgacaccaac ggcggctccc tgcccgacca ggtccgcgcc
     3841 gccatggccg aggtgaagaa aatcacccgc cggccgctgg gcatccacac ccacaacgac
     3901 agcggcctgg cggtggccaa ctcgataacc gcggtggccg agggggccgt ccaggtccag
     3961 ggcaccctga acggcctggg cgaacggtgc ggcaacgccg acctgctggt aatcgtcccc
     4021 atcctccagg tcaagatgaa ccggccctgc ctggatccgg aaaaactggc ccggctgacc
     4081 acgctcgccc attataccta cgaagtggcc aacctgacgc cgcccaacaa ccagcccttt
     4141 gtgggggcct cggccttcgc ccacaagggc ggcatccacg tggacgccat ccgcaaggag
     4201 ccgcgcagtt acgaacacct cgacccggcc ctggtcggca acgaacgccg gatcctggtc
     4261 tccgaacttt cgggcggcgc gaccatcctc ttcaaggccg aacgctacgg catcaacaaa
     4321 aaggggccgg agacccgccg gatcctggcc gaactggccc gcctggagaa ggccggctac
     4381 cagttcgagg ccgccgaggg ctccttccag ctgctggtgg ccgaggtgct cggcaagcgg
     4441 cccgtcttct tccaggtcca ggagttccgg gtcatcgtcg agcggaagaa cggcctgatc
     4501 gtctcggagg cctcggtcaa ggtcaaggtg ggcgaccgcc tcgagcacac cgtcgccgag
     4561 ggagacggcc cggtcaacgc gctcgacgcc agtctgagaa aggcgctgct ctccttctac
     4621 ccctcgctgg ccgacctgcg cctcaccgat tataaggtgc gggtgctcaa cccggaggcg
     4681 gccacggccg ccaaggtccg ggtcctgatc gaatcggccg accgggagga gacctggggc
     4741 acggtcggcc tttcggagaa catcatcgag gccagcctcc aggctttggc cgacagcatc
     4801 gactacaagc tcttcaagga atcggccgtt tcgcgccacc ggccatagag gttattgaca
     4861 aaaccggtta taaaaggtaa aaattatcta tacaggcgct gtcaacccaa acggaaacat
     4921 ccagccggtc tggtgcaatt ttaatcaaaa gggggaggcg tatgaagaaa tggatgcttt
     4981 ggctggttct ggtggcagta gcggctttca tggccggctg tgcctgcaag gtgtcgccgg
     5041 taggcccgcg ggcgatcgtc atcgaccagc cggcggcggt cgtcgagaaa ccggtggtcg
     5101 tcgagaagac ggttgagcgg attatcgaga agccggtgga gaaggtggtc acggtcgaaa
     5161 agatcgtcga gaagccggtg gagaaaatcg tttacgtgga taagccggtg acggtggaga
     5221 cgaaggccta tccaggcatc cgcgatgtct tcttccccta cgacagcgcc gaattgaccc
     5281 cgctga
//
LOCUS       DAOQYN010000134         5281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_15916_length_5281_cov_4.647532, whole genome shotgun sequence.
ACCESSION   DAOQYN010000134 DAOQYN010000000
VERSION     DAOQYN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15916_length_5281_cov_4.647532"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /locus_tag="PKN80_06140"
     CDS             complement(<1..2157)
                     /locus_tag="PKN80_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35628.1"
                     /translation="MKGILLTAVFGILCLTGLLAAPAAGAVRLDIEAEDFDRVPLYPD
                     WHTSSGQGWYAMEQRYFSGYAGLICDAQSVGAETTCTFKEPLPAGRYRFFLYLLKMRS
                     GGKNSLEVAVGDQKWTVTWDENIARVRDYAVVPHDLVLDRPASRITIKSLQIERHNIG
                     DVPSYPIPTIMLDRMVITDDLELQVIESGGRQGVGRPEAESGKPAAAGPALTGNRVAN
                     GSFEVGTTTTWFNFGDPLQPANLVEGAAHGRRCLELPQLSSAFQTAGICSGVFRGAAG
                     EKVPVSLYLKSTAPISVSVAVQNTARREAGVLKAAVTDQWQRFNGEAVLPPDPEGAYS
                     LIIRPEVPGKPVRVFVDAVSVGLAAGADYQARSRYEAGLAGTALANIYHDRERAPAVL
                     TLANHDRKAWPADLEILVRDHRDEVVARQPVKAACPAGSGLERTVNLAAGRWGVFSAV
                     VRDRQGGTDLAELCYINLPPVEGRNNFLGFYGSAWNDRNMAIFKELGIDFVATLADGS
                     ARHIRTDGFDDLDRVLDTPARRGIGVFYATEILYHKPQKDFQWSPSEGVTAFPNPPNL
                     KSLFDDLYAFAGRFAPKIDSWCLQDELYEGKCPLNAWLLYHKTAVEAIRKADPDAVIT
                     VSSEPAFQERVLKLLGPEYLSVVCDPAFSPMTSRRYNHLPFTRLAREYGIPLWFSGFG
                     FPSVSFYRTRAADMRYKNPNYSELVDRTAMVLAANRL"
     gene            complement(2262..3833)
                     /locus_tag="PKN80_06145"
     CDS             complement(2262..3833)
                     /locus_tag="PKN80_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNQ35629.1"
                     /translation="MLRDKILGFQFDLKRAMWREAYFDRMAEILAGWGFNQVLYEFED
                     KLRFRKHPCLAHPDAWSPEATAALVRRLEGRGLEVVPLFQSLGHAESVLRLPEYAHLR
                     QSPEHLNLYDPVSEAARDFLKEIFAETIEVFQPRRFFHVGGDETRNLGAAGRAAGREP
                     GGLYLGHLRPLWEYLLGRGLRPVVWADMLLTYLDVLEQTPAGVVLMDWDYSTGGERWP
                     RLRRWGVGELDWEAYRSREDPEFKSRFEKYFVDERTPADGLFRGFGYVDALVDRGFNV
                     IAASSSRCWGDSAGLPFNQLHLANVFYSARKGRAVGLGTLVTSWAARRHHPELGLPAA
                     FAAAYAGRREGPFDLAAFSREFTAEFYGAAAPEFGRAMELAGVRPSLSESRHLELAAG
                     LLAEGRDPLEVFFPGEKHSELAGRAATARTSLREAREIISGLRDRARRNADNLDFWLE
                     GIDLVAFYADFAAAVLSGRLAAESGRLREDLNRLRLVTRDLFGRTYEAAGLEAELDLR
                     YGVHEALLGKLPAPR"
     gene            complement(3882..>5281)
                     /locus_tag="PKN80_06150"
     CDS             complement(3882..>5281)
                     /locus_tag="PKN80_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35630.1"
                     /translation="RLNRPRDDWAGQAAAIYRQMVRCFFLQRADTVAPYIFRYNSFSA
                     TRSPDYSFFDFDGSFKAQAIGFLMAGNIFSALTPDPEPVYYPFLEYDSCVVYGFNKAG
                     RRGYLLTGNGQRLKLDLPKSGLEILDWFENPVEPAALEGGPQEVILTLSGQPVYLYDR
                     GAGPQRLDEAVRGAQLLPPVDGLLTGPLFLVRDGQLVRAIVTANLDSQPRSGLAAGAG
                     RLDSSAPQANPFLTRPLENEKGAWLLNCLKTARPVRLDGNLPEWSDRSSAWMYVDWGE
                     NSPPTRRQLHILSGGHFINHRPWHDFKVGFSAGYDAENLYFAFQINDDDIAPPEQAGR
                     GLYDGLRLRLDTRPVEDLGRAAEDEDDYLVEVKLPAPDRVNAELVSQGRRQPVTAAVG
                     RHAHGWTVELAVPLKTLGISGSPVGRLLGLNLVASDADYNRKYQGPDQYRPEFSELIW
                     AADNPFRLGSLYFAE"
BASE COUNT          952 a         1749 c         1747 g          833 t
ORIGIN      
        1 caggcggttg gcggccagta ccatggccgt ccggtcgacc agctcgctgt aattgggatt
       61 cttgtaccgc atgtcggccg cccgggtgcg gtagaagctg acgctcggga aaccgaaacc
      121 gctgaaccag aggggtatcc cgtactcccg ggccagccgg gtgaagggca ggtggttgta
      181 acgccggctg gtcatcgggc tgaaggcggg atcgcagacc accgaaagat actccggacc
      241 cagcaatttc aaaacccgct cctggaaggc cggctcggaa ctgaccgtga tgaccgcatc
      301 cgggtcggcc ttgcggatcg cctccaccgc cgtcttgtgg tatagcagcc aggcgttcag
      361 gggacacttc ccctcgtaaa gttcatcctg gaggcaccag gagtcgatct tcggagcgaa
      421 ccggccggca aaggcgtaga gatcatcaaa gagcgatttc aggttgggcg gattcggaaa
      481 ggcggtcact ccctcactgg gcgaccattg gaaatccttc tgcggcttgt ggtaaaggat
      541 ctcggtggcg taaaagacgc cgataccccg gcgggccggg gtgtcgagca cccggtccag
      601 atcatcgaaa ccgtcggtgc ggatgtggcg ggccgatccg tcggcgaggg tggccacgaa
      661 atcgatgccc agttccttga agatggccat gttgcggtcg ttccaggccg agccgtagaa
      721 gcccaggaaa ttgttccggc cttcaaccgg cggcaggttg atgtagcaga gttcggccag
      781 gtcggtcccg ccctggcgat cccgcaccac cgcgctgaaa acgccccagc ggccggcggc
      841 caggttgacc gtccgctcca ggccggaacc ggccgggcag gccgccttga ccggctgccg
      901 ggccacaacc tcgtcccggt ggtcgcgtac caggatctcc aggtcggccg gccaggcctt
      961 ccggtcatgg ttggccaggg tcagaacggc cggcgccctc tcccggtcgt ggtagatgtt
     1021 ggccagggcg gtgccggcca ggccggcctc gtagcgggac cgggcctggt aatcggcacc
     1081 ggccgccagc ccgaccgaaa ccgcgtccac gaagacgcgg accggcttcc cgggaacctc
     1141 cggccggatg atcagcgagt aggccccctc cggatccggg ggcagcacgg cctcgccatt
     1201 gaaacgctgc cactgatcag tcaccgccgc cttgaggacg ccggcctcgc gccgggcggt
     1261 attctggacc gcgaccgaga cgctgatcgg cgccgtgctc ttcaggtaaa gcgaaaccgg
     1321 caccttctcg ccggcggcgc cccggaagac gccgctgcag atgccggcgg tctggaaggc
     1381 gctcgacagc tggggcagtt ccaggcagcg gcggccgtgg gccgcccctt cgaccaggtt
     1441 tgcgggctgg agcggatcgc cgaagttaaa ccaggtcgtg gtggtcccga cctcgaagga
     1501 accgttggcc acccggttgc cggtcaaggc cgggccggcg gccgccggtt tcccggactc
     1561 tgcctccggc cggccgactc cctgccggcc gccgctttct atcacctgca gttccaggtc
     1621 gtcggtaatc accattctgt ccagcatgat ggtcggaatc gggtagctgg gcacgtcgcc
     1681 gatgttatga cgttcaatct gcaggctctt gatggtaatc cggctggccg gccggtcgag
     1741 gaccaggtca tgcgggacga ccgcgtaatc gcggacccgg gcgatgttct catcccaggt
     1801 gaccgtccac ttctggtcgc ctacggccac ctccaggctg ttcttgccac cggaccgcat
     1861 cttgagcagg tagagaaaga agcggtaccg gccggccggc aacggctctt tgaaggtgca
     1921 ggtggtctcg gcgccgaccg actgggcgtc gcagatgagg ccggcgtaac cactgaagta
     1981 acgctgctcc atggcgtacc aaccctggcc gctgctggtg tgccagtccg ggtagagcgg
     2041 cacccggtcg aaatcctcgg cctcgatgtc cagccgcacc gcgccggcgg ccggagcagc
     2101 cagcagaccc gtcaggcata aaatgccaaa gaccgccgtc aacaaaattc ccttcatcct
     2161 tcaccccctg ttattaagat tgaaaatcca agaaatcccg tttgatctta acattgtaaa
     2221 aatttatttt aaccgccggc cgaccggaaa tcaaaaaatt ttcagcgcgg cgcgggtaat
     2281 ttcccgagca gcgcctcgtg caccccgtaa cggaggtcca gttccgcctc caggccggcc
     2341 gcctcgtaag tgcggccgaa cagatcccgg gtgaccaacc gcaatcggtt caaatcctcc
     2401 cgcaaccgac cagactcggc cgccagccga cccgagagca cggcggcggc gaagtccgcg
     2461 tagaaggcga ccaggtcgat cccttccagc cagaaatcga ggttgtcagc gttgcgccgg
     2521 gcccggtcgc gcaggccgga aatgatctcc cgcgcctccc gcagcgaagt ccgggccgtc
     2581 gccgcccggc cggccagttc ggaatgcttc tccccgggga agaaaacctc cagagggtcc
     2641 cgaccctcgg ccagcaatcc ggcggccaac tccaggtggc gggattcgct cagggacgga
     2701 cgaaccccgg ccagttccat ggcccggccg aattcgggcg cggccgcgcc gtaaaattcg
     2761 gcggtgaact cgcggctgaa ggccgccagg tcgaaggggc cttcgcggcg cccggcataa
     2821 gcggcggcga aggcggccgg cagtcccagt tccgggtgat ggcgacgggc cgcccaggag
     2881 gtgaccaggg tgcccagccc gacggcgcgc cccttacggg ccgagtagaa gacattggcc
     2941 aggtggagct ggttgaaggg caaaccggcc gagtcacccc agcaccggct ggacgaggcg
     3001 gcgatgacat tgaacccccg gtcaaccagg gcgtcaacgt agccgaaacc ccggaagagc
     3061 ccgtcggccg gggtccgttc atccacgaag tacttttcga aacgggactt gaactccggg
     3121 tcctcccggc tccggtatgc ctcccaatcg agttccccga ccccccagcg gcgcaggcgc
     3181 ggccaacgct cgccgccggt cgaataatcc cagtccatca ggaccacccc ggccggggtc
     3241 tgctccagca catccaggta ggtcaggagc atgtcggccc agacgaccgg ccgcagtccc
     3301 cggcccagca ggtactccca gagcggccgc aggtggccca ggtaaaggcc gcccggctcc
     3361 cggccggcgg cccggccggc cgcgcccaga ttccgggtct cgtcgccgcc gacatggaag
     3421 aaacgccggg gctgaaagac ctcgatcgtc tccgcgaaaa tctccttcag gaagtcccgg
     3481 gccgcctctg aaaccgggtc gtagaggttc aggtgctccg ggctctggcg caggtgggca
     3541 tactcgggca gccgtaatac ggattcggcg tggcccaggg actggaagag agggaccacc
     3601 tcgaggccgc gcccctccag gcgccggacc agggccgcgg tcgcctccgg gctccaggcg
     3661 tccgggtggg ccaggcacgg gtgcttgcgg aagcgcagct tgtcttcaaa ctcgtaaaga
     3721 acctggttga aaccccagcc ggccaggatt tcggccatcc ggtcgaaata ggcctcccgc
     3781 cacatcgccc gcttcaggtc gaattggaag ccgagtatct tgtctcgaag catcgaaaaa
     3841 ccttcgattc cttgccggaa acaaccggaa aaccgcccgg tttactccgc gaagtaaagg
     3901 ctgcccagac ggaaggggtt gtcggccgcc cagatcagtt ccgaaaattc cggccggtac
     3961 tggtccggcc cctggtactt ccggttgtaa tcggcatccg aggcgaccag gttaagaccc
     4021 agcaaccggc cgaccgggct gccgctgatg cccaaagttt tcaaaggcac ggccaactcg
     4081 accgtccagc cgtgggcgtg gcggccgacg gcggccgtaa ccggctggcg ccgtccctgg
     4141 ctgaccaatt cggcgttcac ccggtcgggc gccggcagct tcacttcgac caggtaatca
     4201 tcctcgtcct cggccgcccg gcccaggtcc tccaccggcc gggtatccag ccgcagccgc
     4261 aggccgtcgt aaagaccgcg gccggcctgc tccggcggcg cgatatcatc gtcattgatc
     4321 tggaaggcga agtagaggtt ttcggcatcg taaccggccg agaagccgac cttgaaatca
     4381 tgccagggcc ggtgattgat gaagtggccg ccggagagaa tgtgcagctg gcgccgggtg
     4441 ggcgggctgt tctcgcccca gtcaacatac atccaggcgg aggaacggtc cgaccactcc
     4501 ggcaggtttc cgtccagccg taccggccgg gccgtcttca ggcagttgag cagccaggcc
     4561 cccttttcgt tctccagcgg ccgggtaagg aacgggttcg cctggggtgc gctgctatcc
     4621 agccggccgg cgccggccgc cagccccgac cggggctggc tgtccagatt ggcggtcacg
     4681 atcgcccgaa ccagctggcc gtcccggacc aggaagagcg gtccggtgag caggccgtcg
     4741 accggcggca ggagttgcgc gccccggacc gcttcgtcca aacgctgcgg tccggcgccc
     4801 cggtcgtaga gatagaccgg ctgcccggag agggtcagga tcacttcctg ggggccgcct
     4861 tccagggcgg ccggctcgac cgggttctcg aaccagtcca gtatctccag gccgcttttg
     4921 ggcaggtcca gcttcagccg ctggccgtta ccggtcagga gatagccccg gcggccggct
     4981 ttgttgaagc cgtaaaccac gcagctgtca tactccagga aggggtaata gaccggctcc
     5041 gggtccggcg tcagggccga gaagatgttg ccggccatca ggaaaccgat ggcctgggcc
     5101 ttgaaggagc cgtcgaaatc gaagaatgag tagtccgggg aacgggtggc cgaaaaggag
     5161 ttgtagcgga agatataggg ggccacggtg tcggcccgct ggaggaagaa acagcggacc
     5221 atctgccggt agatggcggc ggcctggccg gcccagtcgt cccggggccg gttgaggcgg
     5281 t
//
LOCUS       DAOQYN010000135         5281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_15917_length_5281_cov_4.617298, whole genome shotgun sequence.
ACCESSION   DAOQYN010000135 DAOQYN010000000
VERSION     DAOQYN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15917_length_5281_cov_4.617298"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PKN80_06155"
     CDS             complement(<1..845)
                     /locus_tag="PKN80_06155"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNQ35631.1"
                     /translation="MAKKGINKIAVLAGGDSPEREVSLRSGAAVARALESRGFETVTL
                     DPAAPGFAGALLAGGPPRAAFVALHGGSGENGTIQGFLETLGIPYTGSGVLASALAMD
                     KLLSKQIFLASGVPVPPPCGPGELPLVVKPACGGSTLGISIVRTPGQLAAARRLALRY
                     DRRLLMERFIPGVEITVSILGNRRPRILPSIEIVPVGSDFYDYRAKYQSGGSRHIIPP
                     RLPEAFRRRAERAALRAHQVLGCRGLSRSEVIVDREGRPYLLDVNTIPGFTETSLFPD
                     AAAAAG"
     gene            complement(870..1721)
                     /gene="murB"
                     /locus_tag="PKN80_06160"
     CDS             complement(870..1721)
                     /gene="murB"
                     /locus_tag="PKN80_06160"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNQ35632.1"
                     /translation="MNILRGEPLARHTTFRIGGPAEIFAEPADLSEARELLRSARGAG
                     QPVLILGAGSNLLVRDEGFPGLVLSTRRLDWLAAGEGGLSAGCGVRIPVLMGYVVKYG
                     LGGLEFLAGIPGTLGGALVMNAGVVGREIGSAVRTVNVLDESLAERELAAGAAGFGYR
                     SSSLAGYPLVAGVRLALTPRPPAAIRADIAGQVHRRRLTQPYGIPSAGSVFKNPPGDY
                     AGRLIEAAGCKGWREGDALVSDRHANFIVNTGAASARQVLGLIERVRAAVRSRFGIEL
                     ELEIKTV"
     gene            complement(1718..2509)
                     /gene="truA"
                     /locus_tag="PKN80_06165"
     CDS             complement(1718..2509)
                     /gene="truA"
                     /locus_tag="PKN80_06165"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNQ35633.1"
                     /translation="MAAIKLTLAYLGTEFCGWQQQPRRDRLSCQERLESALGTICGRP
                     VRVKAAGRTDAGVHSLGQVAVIDAPVKRSPAEILKGVNALLPPAIRVVRVQRVADEFH
                     PRYQAVSKTYRYLIQNQPVASPFWNACAWFFPDRLDLAVMKRAAGRLEGRHDFSAFQD
                     SGRRVADPVRTLYRCRVARAADPFFGGRRLISIEMTADGFLYKMARNIVGTLVEIGRG
                     KLDPEAVREILVSGDRRRAGPTAPARGLCLVRVDYAKPGAGTDRP"
     gene            complement(2496..3533)
                     /locus_tag="PKN80_06170"
     CDS             complement(2496..3533)
                     /locus_tag="PKN80_06170"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNQ35634.1"
                     /translation="MKGFQVGVVGASGAVGRRMVSILESRRFPVGRLRLFASERSAGQ
                     RLEFAGEPVEIERLTPESFTPDIDLLLFSAGASVSREYAPRAAALGIFVVDNSSAWRM
                     EPGIPLVVPEVNPEALSPESLIIANPNCSTIQMVCALYPLHRAARLRRVVVSTYQAVS
                     GAGQKAIREMEDQLESLRGGREVKPAVFPHQIAFNLIPQIDLFLDNGYTKEEMKMVNE
                     TRKIMGEPSLPVTATCVRVPVLVAHSEAVTVDLEKPLEAGAAREILARAPGVEVIDAP
                     EAGRYPMPLMAAGRDAVYVGRIRRDLSSPNGLSFWVVSDNLRKGAALNAVQIAECLFQ
                     KGFLSSRRGGH"
     gene            complement(3530..3766)
                     /locus_tag="PKN80_06175"
     CDS             complement(3530..3766)
                     /locus_tag="PKN80_06175"
                     /inference="COORDINATES: protein motif:HMM:NF022229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNQ35635.1"
                     /translation="MITKIGFAAGDIWQLLDKQNGPVTFKKICEAIDAPRDFLLMSLG
                     WLAREGHVVMEEIKLPRRGQRDYKVTLRSRKGGE"
     gene            complement(3801..4025)
                     /locus_tag="PKN80_06180"
     CDS             complement(3801..4025)
                     /locus_tag="PKN80_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35636.1"
                     /translation="MIPIDLSLAIGLYLFLALGVLVVFWVTTDSRRRTRDFHLEESHH
                     WQCGLCFHRYVDSVAERFSRCPRCGNLNHR"
     gene            4144..4551
                     /gene="queF"
                     /locus_tag="PKN80_06185"
     CDS             4144..4551
                     /gene="queF"
                     /locus_tag="PKN80_06185"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNQ35637.1"
                     /translation="MAEYTAKEAKRGIRERLPEIEVWPNQFPGYEITVTYPEFTSVCP
                     KTGLPDFGTITIRYQPDRSCLELKSLKFYFLAYRNLGIFYENAVNRILRDIVKAAAPL
                     RAKVTGEFHTRGGMHCLVEATYEAESNLPPRKR"
     gene            4514..>5281
                     /locus_tag="PKN80_06190"
     CDS             4514..>5281
                     /locus_tag="PKN80_06190"
                     /inference="COORDINATES: protein motif:HMM:NF036766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNQ35638.1"
                     /translation="MKLKVTFRPENVEIAALPGLTILELARRANLAISAVCGGRGTCG
                     KCLVRINPAPPAGPLELKRIPAARLAEGYRMACQARVTDRDLEVEVPDESRLFNLKVL
                     VSGLSREVAVHSTLRKYYLELAPPVIEDQRSDWERLRDAAAGRAGLRPAELEVGIYLL
                     RRLPVLLRENNFKVTAVLANRRLISVEGGDTTEHCYGVAFDLGTTSIVGALLDMRTAR
                     ELAVAHRTNPQTSYGADVISRINLTLQDPDGGQRMRTA"
BASE COUNT          932 a         1839 c         1717 g          793 t
ORIGIN      
        1 ccggcggccg cggccgcgtc gggaaagagg ctggtctcgg tgaagccggg aatggtgttg
       61 acgtccagca ggtagggccg cccctcccgg tcaacgatca cctcggaacg ggaaaggccg
      121 cggcagccga ggacctggtg cgcccggagc gccgcccgct cggcccggcg gcggaacgcc
      181 tccggcaggc ggggcggaat gatgtgccgg ctgccgccgg actgatactt ggcccggtaa
      241 tcgtaaaaat cgctgccgac cggcacgatc tcgatgctgg gcaggatgcg cggccgccgg
      301 ttgcccagga tgctgaccgt aatctccacg ccgggaatga agcgttccat caggaggcgc
      361 cggtcgtagc ggagggccag gcgccgggcg gccgccagct ggccgggcgt ccgcacgatg
      421 gagatgccca gggtggagcc gccgcaggcg ggcttgacca ccagcggcag ttcaccgggg
      481 ccgcacggag gcggcaccgg gacgccgctg gccaggaaga tctgcttgga gaggagcttg
      541 tccatcgcca gggcgctggc cagaacgccg ctgccggtgt agggaatccc cagcgtctcc
      601 aggaagccct ggatggtgcc gttttcgccg gagccgccgt ggagggcgac gaaggccgcc
      661 cggggcggcc cgccggccag cagcgcgccg gcgaaacccg gcgcggccgg gtccagcgtc
      721 accgtctcaa aaccgcgcga ttccagcgcc ctggccacgg ccgcgccgct gcgcagcgaa
      781 acctcccgtt ccggggagtc gccgccggcc aggacggcaa tcttattgat cccttttttc
      841 gccatgactc ttggcggcaa accggccgct cagactgttt ttatctcgag ttccagctcg
      901 attccgaacc gggaccggac cgcggcccgg acccgttcga tcagacccag cacctgccgg
      961 gcggaggcgg cgccggtatt gacgatgaag ttggcgtgcc ggtcggagac cagcgcgtcc
     1021 ccctcgcgcc agcccttgca gccggccgcc tcgatgagcc gaccggcgta atccccgggg
     1081 gggttcttga agaccgaacc ggccgacgga atcccgtacg gctgggtcag gcggcgccga
     1141 tggacctgcc cggcgatgtc cgcccggatc gccgccggcg gccgcggtgt caacgccagc
     1201 cggaccccgg ccaccagcgg gtatccggcc agggaagacg accggtagcc gaaaccggcc
     1261 gccccggccg ccagttcccg ctcggccagg ctttcgtcaa gcacgttgac cgtgcgcacc
     1321 gccgagccga tctcccggcc gacgacgccg gcgttcatga ccagggcgcc gcccagcgtg
     1381 cccgggatgc cggccaggaa ttccaggccg cccagcccat attttacaac ataacccatc
     1441 aagaccggga tccgcacccc gcagccggcc gacaggcccc cctccccggc cgccagccag
     1501 tccaggcggc gggtggaaag aaccaggccc gggaacccct cgtcgcggac cagcagattg
     1561 gatccggccc ccaggatcag gaccggttgc ccggccccgc gggccgaccg gagcagctcc
     1621 cgcgcctcgg acaggtcggc cggctcggca aagatttcgg ccgggccgcc gatccggaag
     1681 gtggtgtgac gggcgagcgg ttcgccgcgg agaatgttca tggccgatcc gtcccggcac
     1741 ccggcttcgc gtaatcgacc cggaccaggc agagcccgcg ggcgggcgcg gtcggaccgg
     1801 cccggcgccg gtccccggaa accaggatct cccttaccgc ttccgggtcc agcttgcccc
     1861 gaccgatctc caccagggtg ccgacgatat tgcgggccat cttgtacagg aaaccgtccg
     1921 cggtcatttc aatcgaaatc aggcggcggc cgccgaagaa cggatcggcc gcccgggcta
     1981 cccggcagcg gtaaagcgtc cgcaccggat cggcgacccg gcggccgctg tcctggaagg
     2041 cggaaaaatc gtggcgcccc tccagccggc cggcggcccg cttcattacg gccaggtcga
     2101 gccggtcggg aaaaaaccag gcgcaggcgt tccagaacgg actggccacc ggctggttct
     2161 gaatcaggta gcggtaggtc ttggaaacgg cctggtaacg gggatgaaac tcgtccgcca
     2221 cccgctgcac tcttacaact ctgatagccg gcggcagcaa ggcgttgact cccttgagta
     2281 tctcggcggg actccgcttg accggagcgt caattaccgc cacctggccg aggctgtgca
     2341 cgccggcatc ggtccgtccc gcggccttca cgcgcaccgg ccggccgcag atcgtaccca
     2401 gggccgattc cagccgttcc tggcaggaaa ggcggtcgcg gcgcggctgc tgctgccagc
     2461 cgcagaattc ggttcccagg taggcaagcg taagcttaat ggccgccacg gcgggaagac
     2521 aggaagccct tctggaatag gcattcggcg atctggaccg cgttcaatgc cgcacccttc
     2581 ctcagattgt cgctgaccac ccagaaagaa agcccgttcg ggctggaaag atcgcggcgg
     2641 attcgtccca cgtaaaccgc gtcgcgtccg gccgccatca ggggcatcgg gtaacgcccc
     2701 gcctccggag cgtcgatcac ctcgacgccg ggcgcccggg cgagtatctc gcgggcggcc
     2761 ccggcttcca gcggcttctc caggtccacc gtcaccgcct cgctgtgggc gacgaggacc
     2821 gggacccgga cgcaggtggc ggtaaccggc agggagggct cccccatgat cttgcgggtc
     2881 tcgttgacca tcttcatctc ttccttggtg tacccattat ccaggaagag atctatctgc
     2941 ggtatgaggt taaaggcgat ctggtgggga aagaccgccg gcttgacctc ccggccgccg
     3001 cgcaggcttt ccagctgatc ttccatttcg cggatggcct tctggccggc cccggagacc
     3061 gcctggtagg tggaaacgac cacgcgccgg agccgggcgg cccggtgcag cgggtaaagg
     3121 gcgcagacca tctggatggt ggagcagttc ggattggcga tgatcaggct ttcagggctc
     3181 aaggcctccg ggttgacttc cgggaccacc agcggaatcc cgggctccat ccgccaggcc
     3241 gacgaattgt cgaccacgaa gatgcccagc gccgccgccc ggggggcata ttcccggctg
     3301 acggaggccc cggccgaaaa gagcagcagg tcgatatccg gcgtgaatga ttcgggcgtc
     3361 agccgttcga tttcgaccgg ttccccggcg aactccagcc gctgtccggc cgagcgttcc
     3421 gaggcaaaca ggcgcaggcg ccctaccggg aaacggcgcg actcgaggat ggaaaccatc
     3481 cgccggccga ccgcgcccga ggcgccgacc acgccgacct ggaacccctt cattccccgc
     3541 ctttccggct gcgcagggta accttgtaat cgcgctgccc gcggcgcggc agcttgatct
     3601 cctccatcac cacgtgcccc tcgcgggcca gccagcccag gctcatcagc agaaaatcgc
     3661 ggggcgcgtc gatcgcctcg cagatcttct tgaaggtcac cgggccgttc tgcttgtcca
     3721 gcagctgcca gatatcacca gccgcaaaac cgatcttggt aatcatctcc gcctcctttc
     3781 cctgaatgaa actctcgggc tcaacgatgg ttgagattgc cgcagcgcgg acaacgggag
     3841 aaccgctccg caacgctgtc cacgtaacgg tgaaaacaga gaccgcactg ccagtggtgc
     3901 gattcctcga gatgaaaatc gcgtgtccga cggcggctgt cggtggtcac ccagaagacc
     3961 accagcaccc ccagggccag gaaaagatag aggccgatcg ccagactcag gtcaattgga
     4021 atcattaatc gggtaacata tattttacct taaaaattgc ggacctgtcc accgccggta
     4081 ccggccgcgg cggccgtttt tcggggtttt accggccggc ggaaaaagga tataatatct
     4141 ttcatggctg aatacaccgc gaaagaagcg aagcggggaa tccgcgagcg gctgccggaa
     4201 atcgaggtct ggccgaacca gttcccgggt tacgaaatca ccgtgaccta cccggagttc
     4261 acctcggtct gcccgaagac cggcctgccg gacttcggca ccatcaccat ccgctaccag
     4321 cccgaccgtt cctgcctgga gctgaaatcg ctcaagttct acttcctggc ctaccggaac
     4381 ctgggcatct tttacgaaaa cgcggtgaac cgcatcctcc gggacatcgt gaaggcggcc
     4441 gcgccgctgc gggcgaaggt taccggcgag ttccacaccc ggggcggcat gcactgcctg
     4501 gtggaagcca cctatgaagc tgaaagtaac cttccgcccc gaaaacgttg aaatcgccgc
     4561 cctgcccggg ctgaccatac tggagctggc ccgtcgggcc aacctggcca tttcggccgt
     4621 ctgcggcggc cgcggcacct gcggcaagtg cctggtacgg attaacccgg cgccgccggc
     4681 cggaccgctg gaactcaagc gcatcccggc cgcccggctg gccgagggat accggatggc
     4741 ctgccaggcc cgcgtcaccg accgcgacct ggaggtcgag gtcccggatg aaagccgcct
     4801 gttcaatctc aaggtgctgg tctccggcct gagccgggaa gtggccgtcc attccaccct
     4861 ccgcaaatac tacctcgaac tcgcgccgcc cgttatcgaa gaccagcggt ccgactggga
     4921 acgcctccgg gacgcggccg ccggacgggc cgggctgcgg cccgcggagc tggaagtcgg
     4981 gatctatctc ctgcgccggc tgcccgtcct cctgcgcgaa aacaacttca aggtgaccgc
     5041 ggttctggcc aaccgccgcc tcatttccgt tgaaggcggc gacaccaccg agcactgcta
     5101 cggagtggcc tttgacctcg ggaccacctc catcgtcggc gccctgctgg acatgcgcac
     5161 cgcccgggaa ctggccgtag cccaccggac caatccgcag acgtcttacg gagccgacgt
     5221 gatcagccgg atcaacctga ccctccagga cccggacggc ggccagcgga tgcggacggc
     5281 c
//
LOCUS       DAOQYN010000136         5249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_16045_length_5249_cov_4.275703, whole genome shotgun sequence.
ACCESSION   DAOQYN010000136 DAOQYN010000000
VERSION     DAOQYN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16045_length_5249_cov_4.275703"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="PKN80_06195"
     CDS             <1..1474
                     /locus_tag="PKN80_06195"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HNQ35639.1"
                     /translation="FGYPGFETFRAPNFYLRFTGPWDLDRVECPLMQEWILFRCWQVT
                     DYFRDLAGYARSLNPAIGVDSNGQCIRGVNHAFTHGVDHGAQAAYADAVWDENPDYRS
                     EEHPAAIYPSTVKFRGANFLRRLDKEVLGGYKDEETLAANLTFTGHPGINHAWGYAEP
                     GRRPLNPAQPGVAELLAHYRRHTALYTPVRSAARIGLWRSKLSLAFISTGTYLSACVL
                     EQTLFNHRIPFSIISDGHVNGEKLKEFDLLILPDSQYVSQAQAAALIEFVKKGGSLLI
                     TENSGLYNDEGRRRTSPVFGPLFAGDWKAGSSRSSETWSFDPHQQFITLGEAGQPARA
                     LFGRGRAAYLPKLEYVYRPRTFKSRYNVHYDGIDSRYWKEPYNAAEVLETIQWLAPDS
                     RPVTVYGNPELRLDYLAWPDGRLGAALLRCGPLAGTAEIALGVMSASKPAEGECLIPE
                     RKEPRPLTWVGRSDGYWETRLPEIGRHAVIRWRIVQKKKG"
     gene            1482..3434
                     /locus_tag="PKN80_06200"
     CDS             1482..3434
                     /locus_tag="PKN80_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35640.1"
                     /translation="MEMKRGNGFSRRAFLAGCLFALGVAITEPYSLMMIQPYGMSADF
                     IAGAAVVGLFLLALLINPILSLINRRLAFNSSELLLIYMMMLIASAIPSWGLMSILIP
                     LISGVQYFATPENRWGELVLPHLKPWLTPDLNTATAFFEGTSAGAAAPWFGYLRPLAA
                     WLLLLLLIYFVSICITVILRKQWVENEKLVFPLTILPLEMVRPSEGGGRVPPFFKNRL
                     MWLGFAISFLILSLNGLHYYFPEVPAPVLLNSVRIFRNTMSLSFFLSFPVLGLAYLLN
                     LDIAFSLWLFYLLGVLETGWLNIVGFSLPGPLDPFAEGSTMASFRAMGSMITLVFFGL
                     YLARQHLANVLRKAFRNDPQVDDSDEILSYRTAILGLFIGSALILVWLTLHGLPLLPA
                     LVFLFAALVVLTGLSRIICQAGIGFARSINIPPVFTTYSLPSQAIGPAGYSLLGMQFS
                     WSSDIRTTVMASTANSLKINETGRIRPRLLFLALLAALVLAYAGAAWMMLKVGFEHGA
                     INSQQSWFFRGSMIRYNIGYITDKILHPPTRDIIMPRLGFTGVGMAAMGVLMFLRYRF
                     LWWPIHYIGFPIANSYMMSHAWLSIFLAWLLKSIILRYAGPKVYQRSIPFFLGLVLGQ
                     LTAGGVWLIIDFITKTTGNKLPIGIG"
     gene            3486..4298
                     /locus_tag="PKN80_06205"
     CDS             3486..4298
                     /locus_tag="PKN80_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNQ35641.1"
                     /translation="MKKCVEVPYLRPAEILAAREKTSLIFLPVAPLEWHGPHLPLGTD
                     PLNAYQNALFLARELDGLVMPPLFIGTERERRPEMLKSIGFKPSDYVVGMDFPKNSLP
                     SQYFKEEVFALVLRNQLKLLADDWRFRRIVIANGHGAENQIAVIQRLKKEFEGTRPVR
                     VLLMMPLVNFPGDNKWSHATLEETNATLFHYPESVDLKALPAGKKPLKNLDWAIIDDL
                     TFRGRPAPGFTVRPEEDPRRATPAQAKKQVLRTRANCLRQVRDWLAREAAKA"
     gene            4577..>5249
                     /locus_tag="PKN80_06210"
     CDS             4577..>5249
                     /locus_tag="PKN80_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ35642.1"
                     /translation="MKLRMRSGLGFTLIELLVVIAIIAILAAMLLPALEKARTRAQVS
                     NCTSNLKQIGLAIFMYSNDWEGRMPTLASNEIYNNGVAGGVYYLDVWEKHWMYTLLSE
                     NYLRDPKVFQCLGGAGLENHGSIKWQRYNLTDFDAAGKPWAYPFSYTYNKLILPTTLI
                     QKVPDPQDKIICGDMRNHDSSGWNAMQGETGDFQDLADSHNGVINCLMFDGHVEAIAA
                     TNNVGT"
BASE COUNT          969 a         1805 c         1503 g          972 t
ORIGIN      
        1 cttcggctac cccggcttcg agaccttccg ggcgcccaac ttctacctgc gcttcaccgg
       61 cccctgggac ctggaccggg tcgaatgccc cctgatgcag gagtggatcc tcttccgctg
      121 ctggcaggtc accgactact tccgcgacct ggccgggtac gcccgctccc tcaatcccgc
      181 catcggcgtc gattccaacg gccagtgcat ccggggcgtc aaccacgcct tcacccacgg
      241 cgtcgaccac ggcgcccagg ccgcatacgc cgacgcggtc tgggacgaaa accccgacta
      301 ccggtccgag gaacacccgg ccgccatcta tccctcgacc gtcaagttcc ggggggccaa
      361 cttcctgcgc cgcctcgaca aggaggtgct gggcggctac aaggacgagg agacgctggc
      421 cgccaacctg accttcaccg gccacccggg catcaaccac gcctggggct acgccgaacc
      481 gggccggcgg ccgctgaacc cggcccagcc cggcgtggcc gaactgctgg cccactaccg
      541 gcgccatacc gccctttaca cgccggtgcg cagcgccgcc cggatcggcc tctggcgcag
      601 caagctgtcg ctggcattca tttcgaccgg cacctacctt tcggcctgcg tgctcgaaca
      661 gacgctcttc aaccaccgga ttccgttcag catcatttcc gatggacatg tcaacggtga
      721 gaaactgaag gagttcgacc tgctcatcct gccggacagc cagtacgtga gccaggccca
      781 ggccgccgcc ctgatcgagt ttgtcaagaa gggcggctcg cttctgatca ccgagaactc
      841 cggactttac aatgacgaag gccggcgccg gaccagcccg gtctttggcc cgctcttcgc
      901 cggggattgg aaggccggct cctcccggtc gtccgaaacc tggtccttcg atccgcacca
      961 gcaattcatc accctgggcg aagccggcca gccggcgcgc gctctcttcg gccgggggcg
     1021 ggccgcctac ctgccgaagc tggagtacgt gtaccggccg cgcaccttca agagccgcta
     1081 taacgtccat tacgacggca tcgacagccg ctactggaag gagccataca atgcggccga
     1141 ggtgctggag accatccagt ggctggcccc ggattcaaga ccggtgacgg tctatggaaa
     1201 cccggaactg cgcctcgatt acctggcctg gccggacggc cggctgggcg ccgccctgct
     1261 gcgctgcgga ccgctggccg gaacggcgga aatcgctctg ggagtgatgt cggcctccaa
     1321 accggccgaa ggcgaatgcc tgatcccgga acggaaagaa ccgcggccgt tgacctgggt
     1381 cggccgttcc gacggctact gggagacccg cctgccggag atcggccgcc acgcggttat
     1441 ccgctggcgg atcgtccaga agaaaaaagg gtgagtcaaa tatggaaatg aaacgcggca
     1501 acggtttttc caggcgggcc ttcctggccg gctgcctctt tgccctcggg gtggcgatca
     1561 ccgaacctta cagcctgatg atgatccagc cctacgggat gagcgccgat ttcatcgccg
     1621 gggcggcggt ggtcggcctc ttcctgctgg cgctgctgat caacccgatc ctctccctga
     1681 tcaatcgccg gctggccttc aattcaagcg aactgctgct catctacatg atgatgctga
     1741 tcgcctcggc catccccagc tggggactga tgagcatcct gatcccgctc atctccgggg
     1801 ttcaatactt cgccaccccg gaaaaccgct ggggagaact ggtactgccc caccttaaac
     1861 cctggctgac tcccgacctc aacaccgcca ccgccttctt cgagggaacc agcgccggcg
     1921 cggccgcccc ctggttcggc tacctgaggc cgctggccgc ctggctgctc ctgctgctgc
     1981 tgatttactt cgtcagcatc tgcatcacgg tcatcctccg aaagcagtgg gtggagaacg
     2041 aaaagctggt ctttccgctc accatcctgc ccctggagat ggtccggccg tccgaaggcg
     2101 ggggacgggt accgcccttc ttcaagaacc ggctgatgtg gcttggcttc gccatctcct
     2161 tcctgatcct ctccctcaac ggccttcatt attacttccc cgaagtgccg gcgcccgtgc
     2221 tgctgaactc ggtccggatc ttccgaaaca ccatgagcct gagcttcttc ctgagcttcc
     2281 cggtgctcgg actggcctac ctgctcaacc tcgacatcgc cttttcactc tggctcttct
     2341 acctgctggg cgtgctggag accggctggc tgaacatcgt cggcttcagc ctgcccggcc
     2401 cgctcgatcc cttcgccgag ggttcgacca tggcctcctt ccgggcgatg ggttccatga
     2461 tcaccctggt cttcttcggt ctctatctcg cccgccagca cctggccaat gtcctccgga
     2521 aggccttccg gaacgatccg caggtggacg attcggacga gatcctctct taccgcaccg
     2581 ccatcctggg cctcttcatc ggctcggcac tgatcctggt ctggctgacc ctgcatggcc
     2641 tgccgctgct gccggccctg gtctttcttt tcgccgccct ggtggtcctg accggcctga
     2701 gccggatcat ctgccaggcg ggcatcggct tcgcccgcag catcaacatt ccaccggtct
     2761 tcaccaccta ctccctgccc agccaggcca tcggtccggc cggctacagc ctgctgggca
     2821 tgcagttctc ctggtcttcg gacatccgga ccacggtcat ggcttcgacg gccaactcgc
     2881 tcaagatcaa cgaaaccggc cgcatccggc cccggctcct cttcctggcc ctgctggccg
     2941 ccctggtgct ggcctacgcg ggcgccgcct ggatgatgct gaaggtcggc ttcgaacacg
     3001 gcgccatcaa cagccagcaa tcctggttct tccgcggcag catgatccgc tacaacatcg
     3061 gctacataac cgacaagatc ctccatccgc ccacccggga catcatcatg ccgcggctgg
     3121 gcttcaccgg ggtcgggatg gcggccatgg gcgtcctgat gttcctgcgc taccgcttcc
     3181 tctggtggcc gatccactac atcggtttcc cgatcgccaa cagttacatg atgagccacg
     3241 cctggctctc catcttcctg gcctggctgc tcaagagcat catcctgcgc tacgccggtc
     3301 cgaaggtcta ccagcgtagc atccccttct tcctgggact ggtgctcggc cagctgaccg
     3361 ccggcggcgt ctggctgatc atcgatttca tcaccaagac caccggcaac aagctgccga
     3421 tcggcatcgg ttaaaggccc gggcgcgccg gtattttccg gcgcgtcctc aaaatgaggc
     3481 ggaacatgaa aaaatgcgtc gaggtaccgt acctgcgtcc ggccgaaatc ctggccgccc
     3541 gggagaaaac ttcgctcatc ttcctcccgg tggcgcccct ggagtggcac ggaccccacc
     3601 tgcccctggg caccgacccg ctcaacgcct accagaacgc cctcttcctc gcccgcgaac
     3661 tggacggcct ggtaatgccg cccctcttca tcgggaccga aagggaacgg cggccggaga
     3721 tgttgaaaag catcggcttc aaaccctccg attacgtggt gggcatggac ttcccgaaga
     3781 acagcctgcc cagccagtac ttcaaggagg aggtcttcgc cctggtcctg cgcaaccagc
     3841 tgaaactcct ggccgacgac tggcggttcc gccggatcgt gatcgccaac ggccacggcg
     3901 ccgaaaacca gatcgccgtt atccaacggc tgaagaagga gttcgagggc acccggccgg
     3961 tccgggtgct tctgatgatg ccgctggtga attttcccgg cgacaacaag tggtcgcacg
     4021 ccacgctcga ggagaccaac gccaccctct tccactaccc ggaatcggtc gacctgaagg
     4081 cgctgccggc cggcaaaaag ccgctgaaga accttgattg ggcaatcatc gacgacctga
     4141 ccttccgggg ccggccggcc ccgggtttca cggtccgccc cgaggaggat ccccgccggg
     4201 ccacgccggc ccaggccaaa aaacaggtgc tgcggacccg ggccaactgc ctgcgccagg
     4261 tccgggactg gctcgcccgg gaggcggcga aggcgtaaaa aaacgaaacc gcttccaccg
     4321 gagacaacct taagtaaaac cgcccgtttt gatgctaccc tgctaccccg cccgcctgtt
     4381 tttttaacct gagcattgta taacttgaca aatccgtatt cctgcgacat tatattatct
     4441 ggaagaagta tgagaaccgt caccccggtc gaaggttgaa aggaggggag tcaaacggac
     4501 cggaaagggg aaggaagaat ccagaagaag gcccggaagc ccgggcgtgt ccggagtaat
     4561 ttctgaggga gggtttatga aattacggat gcggagtggt cttggcttca ccctgatcga
     4621 acttctggtg gtcatcgcca tcatcgccat tctggcagcc atgctgctgc cggccctgga
     4681 gaaggcccgg acccgggccc aggtgtccaa ttgcaccagc aacctgaagc agatcggcct
     4741 ggccatcttc atgtacagca acgactggga aggacggatg ccgaccctgg cctccaacga
     4801 aatatacaac aacggtgtcg ccggcggtgt ttactacctt gacgtttggg agaagcactg
     4861 gatgtacact cttctctccg agaactacct ccgggacccg aaggtctttc agtgcctggg
     4921 gggcgccggc ctggaaaatc atggcagcat caagtggcag cgttacaacc tgaccgattt
     4981 cgacgcagcc ggaaagccct gggcctaccc gttcagttat acttataaca agcttatcct
     5041 gcccaccacc ctgatccaga aggtgcccga cccgcaggac aagatcatct gcggggacat
     5101 gcgtaaccac gacagcagcg gctggaatgc gatgcaggga gaaaccggtg atttccagga
     5161 tctggccgac agccacaacg gggtgatcaa ctgcctgatg tttgacggcc acgtggaggc
     5221 gatcgcggcg accaacaacg tcggcacgg
//
LOCUS       DAOQYN010000137         5237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_16088_length_5237_cov_4.099382, whole genome shotgun sequence.
ACCESSION   DAOQYN010000137 DAOQYN010000000
VERSION     DAOQYN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16088_length_5237_cov_4.099382"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKN80_06215"
     CDS             complement(<1..190)
                     /locus_tag="PKN80_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35643.1"
                     /translation="MEETWMEAPLILHSPLNWQVFQRRRRLAGPVLLSGRAGAGCDRL
                     RFRAAGVSVAGSLPERWQE"
     gene            complement(198..935)
                     /locus_tag="PKN80_06220"
     CDS             complement(198..935)
                     /locus_tag="PKN80_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ35644.1"
                     /translation="MAFDYLAHVTFLFYSPGGKVLLTDPFFAGGFEWEGHYETHLTRP
                     AVPVDAIRRCDAVFITHLHGDHCDPAAVRTVWSRTGAPVIAPAEVLERLAAAGIGSRS
                     LVRAEPGRTFEFGDLSLTTLTGYDHFLDHRGRPNKFAACLASGGARVFYSGDCHRVPP
                     DLRGRELDAVFLWPHHQPERLREFRRFVRFEELVLMHGDRFKPGRFLCNKDYRRERRR
                     IERLLPGVRVTIPERIASLLDFKRQLG"
     gene            complement(975..2198)
                     /locus_tag="PKN80_06225"
     CDS             complement(975..2198)
                     /locus_tag="PKN80_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018976497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35645.1"
                     /translation="MNREIRIGLIGYQFMGRAHANAYQQAARFFDPKARSVMKAVCGR
                     HQDGVEAFARRWGFESCETDWRRLVERKDIDLVDITTPNNLHAEAAVAAAEAGKAVFC
                     EKPLALNLEQAETMLRAVSGNRVFNAVCFNYRRAPALALMKKLIAEGRLGRIYHLRGI
                     YLQDWLLDPEFPLAWRMKKEVAGSGSLGDLASHQIDAARWLLGEFSEVCGRLQTFVKE
                     RPVATSEGNLAAVGGSKRGRVTVDDAVLFLARFRNGALGSFEATRFAAGHKNQNRLEV
                     NGEKGSLIFNLERLNELEYYNTDDPEEARGFRLIQVTEPVHPYLSAWWPPGHIIGYEH
                     TFIHTVLDVINAWAAGAPFQPDFKDGLACQRVLAAVSASAASGAWVKLAAPAGTGRRP
                     APKAEKEEGPKLFNA"
     gene            complement(2238..3293)
                     /locus_tag="PKN80_06230"
     CDS             complement(2238..3293)
                     /locus_tag="PKN80_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HNQ35646.1"
                     /translation="MKIRDKTLPALGKIPPEFFDRVIYPRLGAPRPDVIIGPRHGVDY
                     GVIRLADGTCLALSSDPFFIVPAYGFDRAAWFAFHIIVSDVAVSGLEPVYLAVDLNLP
                     PEITALELETMWETVHREAVRYGVNIVTGHTARYAGCNYPMVGGATALAAGPESDLRG
                     PHLVRSGDRLVITKGPAVETTGLLAISFPDRLKAAFGADFQRRAEAIFYQMSVLEDCA
                     VARRFPGVRVMHDATECGIWGGLYEMARAGGFGLLVERERIVTQSVIMETCRHFEIDP
                     FVSISEGTLLVIVAPDQAGALVEAFQARGIPASEAGLVTSPEDGVRVKEAGRVRPLEH
                     PRTDPYWLTVERLLSEG"
     gene            complement(3277..4146)
                     /locus_tag="PKN80_06235"
     CDS             complement(3277..4146)
                     /locus_tag="PKN80_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNQ35647.1"
                     /translation="MKKPNPLWEAFLRNRRLDDCPIYDLHGHMGPFAGIFLSQSELPV
                     MVETLERDGVEQLVFCHHDTLFSPDAGGNRLNIEAVRRFPERLAAYCGLNPNYPEQVA
                     ADLDAFDRYRDVFVGFKLLADYHQVPITDACLRPAWEKADRERLPVLLHTWQNSVYDG
                     PVPVRKIAERYHRVPILLGHSFHRAWDEAVKIAREFDNIYLELTAVPDERGALELFVG
                     ELGSERVIYGTDFPWFSHHYYIGAVLGAGLGEGDCRNIFYRNARRLLDSFQAGRRPGR
                     GQKKTRGKNEDPG"
     gene            complement(4143..4907)
                     /locus_tag="PKN80_06240"
     CDS             complement(4143..4907)
                     /locus_tag="PKN80_06240"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ35648.1"
                     /translation="MKLDWFDFNLYLGRPFRPVFGAVPDAAALQAELRRRGIGRALVW
                     HIAQHDNFPGTGNRLLADELKGKNNLFGCWVVLPPQTGEIIGPDFFPAMRRRRIRALR
                     LFPESHHFLLNRTVFGRFFDEVSERKIPVLLSTMKRGKGVGWPDIYSFLAEYPKLTCV
                     LCDIGIWGVNRYTWPLLERYPKLYLETSCLALEAGGLDAGLARFGPERFLFGSGFPER
                     YPEASQLPLVHAAVSESGRRLVAAGNAEKLLAEVEL"
     gene            complement(4904..>5237)
                     /locus_tag="PKN80_06245"
     CDS             complement(4904..>5237)
                     /locus_tag="PKN80_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35649.1"
                     /translation="VAFQFDEEKRARAFAAAEGVGGWLPFDTGKHVYINWDPVLQHRA
                     GHHPAVNPYNRPENRGLRLDYSMDQLPRTLDILKRTVFVSLHPDWSEAETRTRIAACR
                     RAAAAGEK"
BASE COUNT          946 a         1728 c         1720 g          843 t
ORIGIN      
        1 tctcctgcca gcgttccggg agcgacccgg caaccgagac gccggcggcc cggaaacgca
       61 gccggtcgca gccggccccg gcgcggccgg agagcaggac cggaccggcg aggcgccgcc
      121 ggcgctgaaa aacctgccag ttcaaggggg agtgcaggat cagcggtgct tccatccagg
      181 tttcttccat tattccctca cccgagttgg cgtttgaaat cgagcaggct ggcgatccgt
      241 tccggaatgg ttacccggac gcccggcagg agccgctcga tccgtctccg ctcccgacgg
      301 taatccttgt tgcagagaaa acgccccggc ttgaaacggt ctccatgcat caagaccagt
      361 tcttcaaacc ggacgaaacg gcggaattcc cgcaggcgct ccggctggtg gtgcggccag
      421 aggaaaaccg cgtcaagctc ccggcccctc aagtccgggg gcacccggtg gcagtcgccg
      481 ctgtagaaga cccgggcgcc gccgctggcc aggcaggcgg cgaatttgtt gggacgcccc
      541 cggtggtcca gaaaatggtc gtacccggtc agggtggtca gcgacaggtc tccaaattcg
      601 aaggtccggc ccggctcggc ccggacgaga gaccgggaac ctatgcccgc cgccgccagc
      661 cgttccagaa cttccgccgg ggcgataacc ggcgcgccgg tccggctcca gaccgttcgt
      721 accgccgccg ggtcgcaatg gtcgccgtgg agatgtgtga tgaagaccgc atcgcagcgg
      781 cggatggcgt caaccggaac cgccggccgg gtcaggtgcg tctcgtaatg gccttcccac
      841 tcgaaaccgc ctgcgaagaa ggggtcggtc aaaagaacct tcccgcccgg cgaataaaag
      901 agaaaggtaa cgtgggcgag ataatcgaat gccagcccct tgatgcgcag aggttgcctc
      961 cgtccagact tttttcaggc gttgaagagt ttcggacctt cctctttttc ggccttcgga
     1021 gcgggccgcc ggccggtccc ggccggggcc gccagtttca cccaggcgcc ggaagccgcc
     1081 gaagcggata cggccgccag tacgcgctgg caggccaggc cgtccttgaa atcgggctgg
     1141 aaaggagcgc cagccgccca ggcgttgatg acatcgagca cggtatggat aaaggtatgc
     1201 tcgtagccga tgatgtggcc gggcggccac caggccgaaa ggtagggatg gaccggttcg
     1261 gtcacctgga tgagccggaa accgcgcgcc tcctccgggt catcggtatt gtaatactcc
     1321 agttcattga ggcgttccag gttgaagatc agcgatccct tttcgccgtt gacctccagg
     1381 cggttctggt tcttgtgacc ggcggcaaag cgggtcgcct cgaaggagcc gagcgcgccg
     1441 ttgcggaaac gggccaggaa gagaaccgcg tcgtccacgg tcacccggcc gcgcttgctg
     1501 ccgccgaccg cggccaggtt cccctcactg gtggccaccg gccgttcctt cacgaaggtc
     1561 tggagccggc cgcagacctc gctgaattca cccagcagcc aacgggcggc gtcaatctgg
     1621 tggctggcca ggtcacccag cgaaccgctg ccggccacct ccttcttcat gcgccaggcg
     1681 agcgggaact ccgggtccag aagccaatcc tgcaggtaaa tcccgcgcag gtgatagatc
     1741 cggcccagcc gcccctcggc aatcaacttc ttcatcaggg ccagggccgg ggcgcgccgg
     1801 taattgaagc agacggcgtt gaagacccgg ttcccggaga ccgcccggag catcgtctcg
     1861 gcctgctcga ggttcagggc cagcggcttt tcgcagaaga ccgccttgcc ggcctcggcg
     1921 gcggccacgg ccgcctcggc gtgcaggttg ttgggcgtgg taatgtccac caggtcgatg
     1981 tccttccgtt ccaccagacg gcgccagtcg gtctcacagc tttcaaaacc ccagcgccgg
     2041 gcgaaggcct ccaccccgtc ctggtggcgg ccgcagacgg ccttcatgac cgaccgggcc
     2101 ttgggatcga agaagcgggc cgcctgctgg taagcgttgg cgtgggcccg ccccatgaac
     2161 tggtacccga tcaagccgat ccgaatctca cgattcatca tcgcctccct tttccgcttc
     2221 caaaaagaaa tcaggtatca accttccgac agcaatcgtt ccaccgtcaa ccagtaaggg
     2281 tcggtgcgcg gatgctccag cgggcggacc cggccggctt ccttgacccg gaccccgtcc
     2341 tccggggacg tgaccagccc ggcctccgag gcgggaatcc cgcgcgcctg gaaggcctcc
     2401 accagcgccc cggcctggtc gggggccacg atcaccagca gggtgccctc gctgatggaa
     2461 acaaaggggt cgatctcgaa gtggcggcag gtctccatga tgaccgactg ggtgacgatc
     2521 cgctcccgtt cgaccagaag tccgaaaccg ccggcccggg ccatctcgta aagtccgccc
     2581 cagatgccgc attcggtggc atcgtgcatc acccgcaccc ccggaaatct ccgggcgacg
     2641 gcacaatcct cgagcaccga catctggtag aagattgcct cggcccgccg ctggaaatcg
     2701 gctccgaagg ccgccttcaa acgatccgga aaggagatgg ccagcaaccc ggtggtctcc
     2761 accgccggcc ccttggtgat gaccaggcgg tcccccgacc tgaccaggtg cggtccgcgc
     2821 aggtcggatt ccgggccggc ggccagggcg gtggcgccgc cgaccatcgg gtagttgcac
     2881 ccggcgtaac gggccgtgtg gccggtgacg atgttaacgc cgtaccgaac cgcctcccgg
     2941 tgcaccgtct cccacatggt ctccagctcc aaggccgtga tctcgggcgg caggttgagg
     3001 tcaaccgcca ggtaaaccgg ttccaggccg gaaacggcca cgtcggaaac gatgatgtga
     3061 aaggcgaacc aggcggcccg gtcgaaaccg taagcgggca cgataaagaa gggatcgctg
     3121 gaaagggcca ggcaggtccc gtccgccagc cggataaccc cgtaatcgac cccgtggcgg
     3181 gggccgatga tgacgtccgg acgaggggcg cccaggcggg ggtagatcac ccggtcgaag
     3241 aactcgggcg gtatcttgcc cagcgccggc aaggtcttat cccggatctt catttttccc
     3301 ccgggtcttc ttttggcccc gcccgggccg ccggccggcc tggaaggagt ccagcaggcg
     3361 ccgtgcgttc cggtagaaga tgttccggca gtctccctcg cccaggccgg cgcccagcac
     3421 ggcgccgatg tagtagtggt ggctgaacca gggaaagtcg gtgccgtaga tcacccgttc
     3481 cgaaccgagt tccccgacga agagttccag ggccccccgc tcgtcgggaa ccgcggtcaa
     3541 ttccaggtag atgttgtcaa attcgcgggc gattttgacc gcttcgtccc aggcccggtg
     3601 gaaggaatgg cccagcagga tcggcacccg gtgataccgc tcggctatct tccggaccgg
     3661 gaccgggccg tcgtagacgc tgttctgcca ggtgtgcagc agaaccggca gccgctcgcg
     3721 gtccgccttc tcccaggccg gccgcaggca ggcgtcggta atcgggacct ggtggtaatc
     3781 ggccagcagc ttgaaaccga cgaagacatc ccggtagcgg tcaaaggcgt cgaggtcggc
     3841 ggccacctgc tccggatagt tcgggttgag gccgcagtag gcggccagcc gttccggaaa
     3901 acgccggacc gcttcgatgt tgaggcggtt tccaccggca tcgggcgaaa agagggtgtc
     3961 gtggtggcag aagaccagct gctccacccc atcccgttcc agcgtctcga ccatgaccgg
     4021 cagttcgctc tggctgagga agatgcccgc gaagggtccc atgtgcccgt gcagatcgta
     4081 aatcgggcag tcgtccagcc ggcggttgcg gaggaaggcc tcccagagcg gattcggctt
     4141 cttcataatt ccacctcggc cagaagtttt tcagcgttgc cggcggccac caggcggcgg
     4201 ccggattccg aaacggccgc gtggaccagc ggaagctgtg aggcctccgg gtaccgttcc
     4261 gggaaaccgc ttccgaagag gaagcgctcc gggccgaacc gggccaggcc ggcgtcgagc
     4321 ccgccggcct ccagcgccag gcagctggtt tccaggtaaa gtttggggta acgctccagc
     4381 aggggccagg tataacggtt gacgccccag attccgatat cgcagaggac gcaggtaagc
     4441 ttcgggtact cggccaggaa ggagtaaata tccggccagc caactccctt ccccctcttc
     4501 atcgttgaaa gcaggaccgg gatcttccgc tccgaaacct cgtcgaagaa acggccgaag
     4561 acggtccggt tgagaaggaa atggtgcgat tccgggaaga ggcgcagggc ccgtatccgg
     4621 cggcgccgca tcgccgggaa gaaatccggg ccgatgatct ccccggtctg gggcggcagt
     4681 accacccagc agccgaaaag gttattcttt cccttcaatt catccgccag caggcggttg
     4741 ccggtaccgg gaaagttgtc gtgctgggcg atatgccaga ccagggcccg gccgattccc
     4801 cgccggcgca actcggcctg gagagcggcc gcgtccggga cggcgccgaa gaccggccgg
     4861 aacggccggc ccaggtaaag gttgaagtcg aaccagtcca gtttcatttt tcccccgccg
     4921 cggccgcccg gcgacaggcg gcgatccggg tccgggtctc cgcctcgctc caatccgggt
     4981 gcagggaaac gaagaccgtt ctcttcagga tgtccagggt ccgcggcagc tggtccatcg
     5041 agtaatcgag tcgcagcccc cggttctccg gccggttgta gggattcacc gccgggtggt
     5101 ggccggcccg gtgctggagg accggatccc agttgatgta aacgtgcttg ccggtgtcga
     5161 agggcagcca gccgcccacg ccctcggcgg ccgcgaaagc gcgggcccgc ttctcttcgt
     5221 cgaactggaa ggcgacc
//
LOCUS       DAOQYN010000138         5231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_16108_length_5231_cov_3.849691, whole genome shotgun sequence.
ACCESSION   DAOQYN010000138 DAOQYN010000000
VERSION     DAOQYN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16108_length_5231_cov_3.849691"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..669)
                     /gene="phoU"
                     /locus_tag="PKN80_06250"
     CDS             complement(4..669)
                     /gene="phoU"
                     /locus_tag="PKN80_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNQ35650.1"
                     /translation="MKRQMDLEMQELKRQLILMDNRAEESITKASRALEQLSPELAQE
                     VISSDTEIDRMENELEEKAVVFIARHQPLASDLRFIVMVSKIASELERIADLAVDIAQ
                     RALELVGQPHIKPLIDTPKLTRLAEQMVHKSIEAFVNLDGELARFVRSQDDQADFLRD
                     SIYRELTEIISRNGSLAPRAIPLILVARHLERICDHATNIAEDVVFLAEAKNIKHSGA
                     GAA"
     gene            complement(698..1456)
                     /gene="pstB"
                     /locus_tag="PKN80_06255"
     CDS             complement(698..1456)
                     /gene="pstB"
                     /locus_tag="PKN80_06255"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNQ35651.1"
                     /translation="MDEIKIEIERLNLHYGNFQALREINAAFPAGRITALIGPSGCGK
                     SSLLRCLNRMNDLVPGARIQGRISLDGEEITGPEIDLISLRKRVGMVFQRPNPFPLSV
                     RDNVTYGLRIHNLAPEADWDGIVEESLRRAWLWRELKDRLRDNALSLPLDQQQRLCVA
                     RLLAVKPEVLLLDEPASALDPIATEKLEELLRVLKQDYTVVIVTHNMDQASRISDFCG
                     FFLLGEMVEFGETEAMFRKPARPETESYITGRFG"
     gene            complement(1464..2222)
                     /locus_tag="PKN80_06260"
     CDS             complement(1464..2222)
                     /locus_tag="PKN80_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNQ35652.1"
                     /translation="MADPIIRTEGLGLTGNGDRLLGSISIELERNTILGIIGPTGSGK
                     TSFLRVINRLIEFDHGYRVSGRVYFEGRDIYQPEVDLAWLRRQIGMVFALPMPLPMSI
                     YENLTFGPRLNGIRNRAALEELVENSLRSAYLWEEVKDRLELPALKLSGGQQQRLCLA
                     RILALKPRVVLLDDPCSGLDPISTSRIEEAIIRLKKHFSFILVTNNTKQVSRVADRTA
                     FFLYGELIETGPTAKIFTAPADRRTNDYITGRFG"
     gene            complement(2215..3063)
                     /gene="pstA"
                     /locus_tag="PKN80_06265"
     CDS             complement(2215..3063)
                     /gene="pstA"
                     /locus_tag="PKN80_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNQ35653.1"
                     /translation="MRLLPHNPDRDQRIMESGCRLMAALVLSILLFIIGFILARGLPQ
                     LKPAFIFGQPEMMGERGGIFPTLLGTFLLPLVALLIATPLGVGTAVYLTEYTRPNRLT
                     AVIRFGTECLAGIPSIIFGLFGFVLFVVTLKLGWSILSGGLTLAVMVLPTIIRTSEEA
                     IGTVPRHYREVALSLGATRWQAVTRVVLPSAFPGILTGIMLAFGRSIGETAAVIFTAG
                     ISLKRPLSALDSVRTMAVHFYILVREGISLPNAYGTAALLILLILAVNVATYWLMHRF
                     LRRIHG"
     gene            complement(3060..3914)
                     /gene="pstC"
                     /locus_tag="PKN80_06270"
     CDS             complement(3060..3914)
                     /gene="pstC"
                     /locus_tag="PKN80_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNQ35654.1"
                     /translation="MRREVAGRILLIIACSSVFMLAAITFFIFREGLPLIIKTGPAAF
                     LAGRDWAPSRGLFGIRPMILSSVWITLGALLLGIPTGVACAIYLAELAGPRLRRILKP
                     AIELLAGIPSVVYGFMGVAILVPLIRNLLGGPGFSLLAGCVILAVMILPTIISISTDS
                     IRAVPLDFREASISMGANRWQTTFHVVLPAARNGILASIILGMGRAIGETMAVIMVAG
                     NALTIPRSPLDPVRPLTSNIALEMGYATGDHQKALFATGIVLFIFILVLNILATVTAR
                     LGAGELKR"
     gene            complement(3911..4735)
                     /locus_tag="PKN80_06275"
     CDS             complement(3911..4735)
                     /locus_tag="PKN80_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ35655.1"
                     /translation="MRYQFRTPYIALLCLAAMLNGCAAPRKVILAGSTSIQPFAELWS
                     DEYAKERPEVRVIVQGGGSSAGVEAARSGTADIGMSSRQLKPEEEFGNDGAPRLKKII
                     VAYDAIAVIVNARSPLTGISSAELRDIFSGRTYSFRGRRLTVVTREEGSGTRASFEEA
                     VMKADDGRTTLPISNSALVQDSNGAVREIVAGDPNAIGYISLGLVDRRVRALSLDGVV
                     PGEAAVRAGTYRLVRPFLFLVRPETKSPDVREFINFCLSPEGQEIVARQGLLKVFR"
     gene            4882..>5231
                     /locus_tag="PKN80_06280"
     CDS             4882..>5231
                     /locus_tag="PKN80_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNQ35656.1"
                     /translation="MCRIILGGTGGQGVITLGKLLAIAGMNEGFNVSCYPIYGAEMRG
                     GYAFSTVILAREEVPSPIISRAELGIFLDPLAFDYLAGMVGRRGMLVINSEQVAVACR
                     RDRRIVPAEALSAAR"
BASE COUNT          901 a         1639 c         1808 g          883 t
ORIGIN      
        1 agctcaggcg gcgccggcgc cggagtgctt gatattcttg gcctcggcca ggaagaccac
       61 gtcttcggcg atgttggtgg cgtggtcgca gatgcgttcg aggtggcggg cgaccaggat
      121 gagcgggatg gcccggggcg ccaggctgcc gttccggctg atgatctcgg tcagctcgcg
      181 gtagatcgaa tcgcgcagga agtccgcctg gtcatcctgg ctgcggacga accgggccag
      241 ctccccgtca agattgacaa aagcctcgat cgacttgtgg accatctgtt cggccagccg
      301 ggtcagcttg ggggtatcga tcagcggctt gatgtggggc tggccgacca gctccagggc
      361 gcgctgggcg atatccaccg ccaggtcggc gatccgttcc agctcgctgg cgatcttgga
      421 aaccatcacg ataaagcgca ggtcgctggc cagcggctgg tggcgcgcga tgaagacaac
      481 cgccttttcc tccagctcgt tctccatccg gtcgatttcc gtgtcgctgc tgatgacctc
      541 ctgggccagc tcgggcgaga gctgctccag cgcccgggaa gccttggtga tcgactcctc
      601 ggcccggttg tccatgagta tcagctgacg cttgagttcc tgcatctcca ggtccatctg
      661 cctcttcatt tggcaacctc cccttgattg gcccgcctca tccgaaccgg ccggtgatat
      721 agctttcggt ctcgggccgg gccggcttcc ggaacatcgc ctcggtctcg ccgaattcga
      781 ccatttctcc gagcaggaag aaaccgcaga aatcggatat gcgcgaggcc tggtccatgt
      841 tgtgggtgac gatgaccacc gtgtaatcct gcttgagcac ccgcaggagc tcctcgagtt
      901 tctcggtcgc gatcgggtcg agggcgctgg ccggttcgtc cagcagcagc acctccggtt
      961 tcacggccag cagccgggcg acgcaaaggc gctgctgctg gtccagcggc agggagaggg
     1021 cgttgtcccg gagccggtcc ttcaattccc gccagagcca ggcccggcgc aggctctcct
     1081 ccacgatccc gtcccagtcc gcctccgggg ccaggttatg tatgcgcagg ccgtaggtga
     1141 cattgtcccg gaccgataaa gggaaggggt tcggcctctg aaagaccatg cccacccgct
     1201 tgcgcaggct gatcaggtcg atttccggcc cggtgatctc ctccccgtcc aggctgatcc
     1261 gcccctggat ccgggccccc ggcaccaggt cgttcatccg gttcaggcag cgcagcaggc
     1321 tcgatttacc gcagccggag gggccgatga gggcggtgat ccggccggcc gggaaggcgg
     1381 cgttgatctc ccggagcgcc tggaaattac cgtaatggag gttcagccgt tcgatctcga
     1441 tcttgatctc gtccatgggc ggctcagccg aagcggccgg tgatgtaatc gttggtgcgc
     1501 cggtcggccg gcgcggtgaa gattttcgcg gtggggccgg tctcgatcag ctccccgtaa
     1561 aggaagaaag cggtccggtc ggccacccgg ctcacctgct tggtgttgtt ggttaccagg
     1621 atgaaggaga agtgtttctt cagccggatg atggcctcct cgatccggct ggtggagatg
     1681 ggatccaggc cgctgcaggg atcgtccagc aggaccaccc tgggcttcag ggccaggatg
     1741 cgggccaggc agaggcgctg ctgctgcccg ccggagagtt tcagggccgg gagttccagc
     1801 cggtccttga cctcctccca gagataggcc gagcgcaggc tgttctcgac cagttcctcc
     1861 agcgcggccc gattccggat gccgttcagg cgtggtccga aggtcaggtt ttcgtaaatg
     1921 gacatcggca gcggcatcgg cagggcgaag accatcccga tctggcggcg cagccaggcc
     1981 aggtcgacct ccggctggta gatgtcgcgt ccctcgaaat agacccggcc gcttacccgg
     2041 tagccgtgat cgaactcgat caggcggttg atcacgcgca ggaaggaggt tttgccgctg
     2101 ccggtcggac cgatgatgcc caggatggtg ttgcgctcca gctcgatcga gatggagccg
     2161 agcagccggt cgccgttgcc ggtcaggccg aggccctcgg tccggatgat cgggtcagcc
     2221 atggatccgc ctcaggaaac ggtgcatcag ccagtaggtg gccacgttga ccgccaggat
     2281 gagcaggatc aggagcgctg ccgtgccgta agcgttcggc aggctgatgc cctcccggac
     2341 caggatgtag aagtggacgg ccatggtgcg gaccgagtcc agggccgaga gcggccgctt
     2401 gagcgagatg ccggcggtga agatgacggc ggccgtctcc ccgatcgagc ggccgaaggc
     2461 cagcatgatg ccggtcagga tgcccgggaa ggcgctcggc agcaccaccc gggtgacggc
     2521 ctgccagcgg gtggcgccca aagagagggc cacctcccgg taatggcgcg gtaccgtccc
     2581 gatcgcctcc tcgctggtcc ggatgatggt cggcagcacc atcaccgcca gggtcaggcc
     2641 gccggagagg atcgaccagc caagttttag ggtgaccacg aagagcacga acccgaagag
     2701 gccgaagatg atcgacggga tgccggccag gcactcggtc ccgaagcgga tgaccgcggt
     2761 gaggcggttc ggccgggtgt actcggtcag gtaaacggcg gtgccgaccc cgagcggggt
     2821 ggcgatcagc agggcgacca ggggcagcag gaaggtgccc agcagcgtcg ggaagatgcc
     2881 gccgcgctcg cccatcatct ccggctggcc gaagatgaag gccggtttca actgcggaag
     2941 gccgcgggcg aggatgaacc cgatgatgaa gagcaggatc gagagcacca gggccgccat
     3001 cagccggcag ccggactcca tgatccgctg gtcccggtcc gggttgtggg gcaggagtct
     3061 catcgtttca gctccccggc gcccagccgg gcggtgacgg tggccaggat gttgagcacc
     3121 aggatgaaga tgaagagaac gatgccggtg gcgaagagcg ccttctggtg atccccggtg
     3181 gcatagccca tctccagggc gatgttgctg gtcagcggcc gcaccgggtc cagcggcgaa
     3241 cggggaatgg tcagggcgtt gccggccacc atgatcaccg ccatcgtctc accgatcgcc
     3301 cgccccatgc ccaggatgat cgaggccagg atcccgttgc gggcggccgg caggaccacg
     3361 tggaaggtgg tctgccagcg gttggccccc atcgagatcg aggcctcccg gaagtcgagc
     3421 ggcaccgccc ggatggagtc ggtggagatg ctgatgatgg tcggcaggat catcaccgcc
     3481 aggatgacgc agccggccag cagggagaag ccggggccgc ccaggaggtt gcggatcaac
     3541 gggaccagga tggccacgcc catgaagccg taaaccaccg aggggatgcc ggccagcagc
     3601 tcgattgccg gcttcagtat ccgccgcagg cgggggccgg ccagttcggc taggtagatg
     3661 gcgcaggcca ccccggttgg aatgcccagc agcagcgctc ccagcgtgat ccagaccgag
     3721 gagaggatca tcggccggat gccgaagagg ccgcgggagg gggcccagtc gcggccggcc
     3781 aggaaggcgg ccggcccggt cttgatgatc agcggcagcc cctcccggaa gatgaagaag
     3841 gtgatcgccg ccagcatgaa gaccgaggag caggcgatga tcagcaggat ccggccggct
     3901 acttcgcgcc tcatcggaag accttcagca gtccctggcg ggccacgatc tcctgccctt
     3961 cgggcgagag gcagaagttt atgaattccc ggacgtccgg ggatttcgtt tccggccgga
     4021 ccaggaagag gaaggggcgg accagccggt aggtcccggc gcggaccgca gcctcgccgg
     4081 gcaccacccc atccagggag agggcccgga cccgccggtc caccaggccc agcgagatgt
     4141 acccgatggc attgggatcg ccggccacga tctcccggac cgcgccgttg gagtcctgga
     4201 ccagggcgga attggagatg ggaagcgtcg tccggccatc gtcagccttc atcaccgcct
     4261 cttcaaaact ggcccgggtt cccgaaccct cctcgcgggt gaccacggtc agccgccggc
     4321 cccgaaacga gtaggtccgg ccggagaaga tgtcccggag ttcggcgctg gagatgccgg
     4381 tcagcgggga gcgggcgttg acgatgaccg cgatggcgtc gtaagcgact atgatctttt
     4441 tcagccgggg cgcgccgtcg ttcccgaact cctcctcggg tttcagctgg cgcgaagaca
     4501 tcccgatgtc ggcggtgccg gaccgggccg cctcgacgcc ggcgctggaa ccgccgccct
     4561 ggacgatcac ccggacctcc ggccgttctt tggcgtattc gtccgaccag agttcggcga
     4621 agggctggat cgaggtggac ccggccagaa tgaccttgcg gggcgcggca cagccgttaa
     4681 gcattgccgc caggcagagg agggcgatgt aaggtgttcg gaattggtag cgcatcggtg
     4741 gaaatccgcg cttagattat acaccatggg aaccgggcgg aggaaatcgc gctccttcgg
     4801 atcgccgttc cggccttgtg ctataatatc agcagttttc acgaaccacg attctttttc
     4861 cggtccacga ctggaacctc tatgtgccga atcatacttg gcggtaccgg cggccagggc
     4921 gtcatcaccc tgggcaaact cctggccatc gccggcatga atgaggggtt caacgtcagc
     4981 tgctacccga tctacggcgc cgagatgcgc ggcggctatg ccttctccac cgtcatcctg
     5041 gcccgcgagg aggtcccctc gccgatcatt tcgcgggcgg aactgggcat cttcctggat
     5101 ccgctggcct tcgactacct ggccggaatg gtcggccgcc gcgggatgct tgtcatcaac
     5161 tccgaacagg ttgccgtggc ctgccgccgg gaccggcgga tcgtgccggc ggaggctctt
     5221 tccgccgccc g
//
LOCUS       DAOQYN010000139         5197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_16245_length_5197_cov_5.231233, whole genome shotgun sequence.
ACCESSION   DAOQYN010000139 DAOQYN010000000
VERSION     DAOQYN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16245_length_5197_cov_5.231233"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKN80_06285"
     CDS             <1..431
                     /locus_tag="PKN80_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35657.1"
                     /translation="YYSKNQYTKYPALEQEIGRVNQELQAIKSYLKIGEPFAGLAVAS
                     RPEVLAKSIVAGDRGLVLILINTGYRTLIRDEKRETTFTPRENFTVEVEMPGWLEIKQ
                     AVEIGPETKSVRFQRRNGKIIIPVDRLELTRIFLLTAGGS"
     gene            433..2640
                     /locus_tag="PKN80_06290"
     CDS             433..2640
                     /locus_tag="PKN80_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35658.1"
                     /translation="MRRLLYLAWFLLLMLAPQLEAALNVSIESPLDDSCFRVDTAILV
                     KARYTCDQPGCSLIFKVDGIQKSSVSASQGVDIQQVFVWDANSPGVFTLTVELRKTGC
                     YSASASIEATIFSVGISGKSLLAKGKSTSLTAAPTPGGLNGTYSWSVIQGSDKINLAG
                     SGNTISVNGLNVSGMINDVTVKVEFTAAGASAAAAATHSLTVFDLEISEGGPNPPDPY
                     IPAGGKRTYLAIVAPTALDEAGPYTWSKSPSETAVISLYPVGYSCEVRTDTYQTGAAA
                     LKARFDPGEGAYAEDSLEILVFRPNIVVGEIGEEAETDTGAYLAKGGTRSCLLQLEGT
                     LPVDETVTLTCDKTGKVSLWEGETQKSFPYQCAVSALPKNLALKGEAVSDTIRDITLT
                     LTTSSGGEDKVKATVVRAEITVFRPQTFPFNEYEIPEDKEENPGAGIRINGDDDNEND
                     VADREDVSVDSENDLIKVKLEVIGSMSAPTGFEYVLKRSNGNIKVWENGTKGTAVLNT
                     NDEQVLTFSGSTKDVWVESPNGGTADLYFQVRRISDGVISCEDKVHFYGFSSCIIVLG
                     GEGQTPSDDSEYGTFILGKILYLDGYDVHMFDEDKTYTEVEPEVRSAIEKRGVITMIV
                     FGYSHGGGSTYIIAKYLVDEELATENTIVYTAYTDAVTNTLPWFAPETRRPPGSLYLT
                     NRYELNTFLIHGDSVPNTEDEVNLTDLDLTHYNIDDYEEVINNIRSVISGFSP"
     gene            2676..3608
                     /locus_tag="PKN80_06295"
     CDS             2676..3608
                     /locus_tag="PKN80_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35659.1"
                     /translation="MKNRFWRIVLIVFLALFLKSTWVSGNKMIAVDTKIQDQINHVVA
                     VEHIASRKEEILLEIKKLKEMGTAEHLLQQLIYFRANMRKVKDAGQITSDRMESGMNT
                     TLYIIGTFLTPTDQSFQKISSLSAQFLIKAVVPYLETEDPNLKKELYRILKIVDLDVA
                     GKQVHPKYSEYPVDYTEYKLFMEVNRDNLSEPLVQYMYQKSPGDALLTLAQVYLKDST
                     EKDSVIQAVAIIREDFWKRYHGPAESRPRIISVKAMQALDQLSARYPQWWIQMYVVEI
                     ILREPRFINPQILERLKNAKHPFILEKLEKTSGK"
     gene            complement(3855..4889)
                     /locus_tag="PKN80_06300"
     CDS             complement(3855..4889)
                     /locus_tag="PKN80_06300"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNQ35660.1"
                     /translation="MKLVSRYLLKEFQTTGLPALGFVLFLLSLGQLFRLVELLVKKIL
                     PAFQVLEYWFWQLLALLPYAFSLAVLIAVTLIFSRLAQDREYLALKGAGISVGRIAAP
                     LLMIGLLLSAGHLGLTGVIEPNALYQGKLLLERSRFNPQTSLIKPGSVVTEFPDIVIF
                     IPRKDSQARVTIYQRDRDQVRTFSARRLRPVWRWGKLNLMLEDGVIQTYQPEKPETFQ
                     KLSFSTYLFPLPRNLPGFKAPKRKLIEETLAGLARLGSPAARVEIMRRTAFAFTPLLF
                     ILIGIPLGIVVHKNLWGVAAACGLVLGYYFAMSGLQALAGLKTGLAALFFLPDLCLLG
                     AGLYFLGRLD"
     gene            5012..>5197
                     /locus_tag="PKN80_06305"
     CDS             5012..>5197
                     /locus_tag="PKN80_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNQ35661.1"
                     /translation="MEKIIRFGVSLPAELLKHFDRESGRRRYANRSEAIRDLMRDWLV
                     RRAWSDSRSEVVGPGTLV"
BASE COUNT         1302 a         1267 c         1507 g         1121 t
ORIGIN      
        1 cctactacag caagaaccag tacaccaaat acccggccct ggagcaggag atcggccggg
       61 tcaaccagga actccaggcc atcaaatcct acctgaagat cggcgagccg ttcgcgggac
      121 tggcggttgc ctcccggccg gaggttttgg cgaaaagcat cgtcgccggc gaccggggcc
      181 tggtgctgat tctgatcaat accgggtacc ggaccctcat ccgggacgag aaacgggaga
      241 cgaccttcac gccccgggag aacttcacgg tggaggttga gatgcccggg tggctggaga
      301 tcaaacaggc ggtggagatc ggcccggaga ctaaatcggt ccggttccag cgccggaatg
      361 ggaagataat catcccggtt gacaggctcg agctgaccag gatctttctg cttaccgctg
      421 gaggttcctg agatgaggcg attactttac ctcgcttggt ttctcctcct gatgctggcc
      481 ccgcaactgg aggcggccct gaacgtttcc atcgagagcc cgcttgacga tagttgtttc
      541 cgggtggaca cggcgatttt ggtgaaggcg cgatacacct gcgaccaacc aggctgctcg
      601 ctgatcttca aggttgatgg aatacaaaag tcttccgtca gcgccagcca gggtgtggat
      661 atccagcagg ttttcgtctg ggacgccaat tctccgggcg tttttaccct tacggtggaa
      721 ttgcgcaaga ccggctgcta cagcgcctcc gccagtatcg aagcgaccat attttccgtc
      781 ggcatctccg ggaagagcct gctggcgaaa gggaagtcaa cttcccttac cgccgcgccc
      841 acgcccggcg gattgaatgg gacttacagc tggtcggtca tccaggggag tgataaaata
      901 aaccttgcgg gcagcgggaa cacgatcagc gtgaacgggc tcaacgtaag cggcatgata
      961 aacgacgtga cggtcaaggt ggaattcacc gccgccggcg cgagcgcggc cgccgcggcg
     1021 acgcattccc tgacggtctt cgacctggag atttccgagg gagggccgaa cccgcctgat
     1081 ccgtacatcc cggccggggg caagcgaacc tacctggcga tcgtggcccc gaccgccctg
     1141 gacgaggccg gcccttatac ctggagcaaa tcgccctcgg aaacggcggt gatcagtctt
     1201 tatccggtgg ggtacagttg cgaggttcgg accgacacct accagaccgg ggccgccgcc
     1261 ttgaaggccc ggttcgatcc cggggagggc gcgtacgcgg aagacagcct ggaaatcctc
     1321 gtatttcggc cgaacatagt ggtgggtgaa atcggcgagg aggcggagac ggacaccgga
     1381 gcctatctgg ccaaaggcgg gaccaggtcc tgccttctgc aactggaggg gactcttccg
     1441 gtggatgaga ccgttactct aacctgcgac aaaaccggca aggtctccct ctgggagggc
     1501 gagacgcaga aatccttccc gtaccagtgc gcggtttccg ccctgcccaa gaacctggcg
     1561 cttaagggcg aggcggtttc ggataccatc agagatatca ccctgacgct gactacctcc
     1621 agcggcgggg aagataaagt caaggcaacg gtggtcagag cagagataac tgtttttcgt
     1681 ccacaaacct ttccttttaa cgagtatgaa attccggaag acaaagaaga aaaccctggt
     1741 gcaggtattc ggataaatgg ggacgatgat aacgaaaacg atgttgcgga tcgggaagac
     1801 gtctcggttg acagtgagaa tgacttaatt aaagttaaat tggaagtcat aggatccatg
     1861 tccgcaccta caggatttga gtatgtttta aaacgtagca atggtaatat aaaagtgtgg
     1921 gagaatggga ctaagggaac ggcagttctt aatacaaatg atgaacaggt gctgactttc
     1981 agtggaagta caaaagacgt ctgggtggaa agtcctaacg gtgggaccgc agatttatat
     2041 tttcaggtaa gaagaatttc agatggagtc ataagttgcg aagataaagt ccatttttat
     2101 ggtttttcga gttgtattat tgttttagga ggagaagggc aaactcccag cgatgattcg
     2161 gagtatggga cctttatttt aggcaaaatc ctatatttgg atggttacga tgttcatatg
     2221 tttgatgaag ataaaactta tactgaagtg gaacccgaag tccgatcggc aattgaaaaa
     2281 cgcggtgtta tcaccatgat tgtttttggt tatagtcatg gtggaggttc gacttatatt
     2341 atagcaaaat atttagtcga tgaagaattg gcaacagaga ataccattgt ctatacggca
     2401 tatactgatg ccgtaaccaa tactcttcca tggtttgcac cggaaacgag aagaccgcca
     2461 ggtagtctgt atttaaccaa tcggtatgag ctcaacacgt ttctaattca cggtgattca
     2521 gttccgaaca cggaagatga agtgaatttg accgatctgg atttaactca ttacaacata
     2581 gacgattatg aggaagtaat taataacatt cgatcggtta tttcgggttt ttctccctga
     2641 caagttctcg ggtaaaaaca aacaaagagg attatatgaa aaacagattt tggcgaatag
     2701 tattaattgt ttttttagcg ttgtttttaa aatctacttg ggtaagtgga aacaagatga
     2761 ttgcggtgga cacgaagata caagaccaga ttaatcatgt tgttgcggtg gaacatatcg
     2821 cgagtcgaaa agaagaaata ttgttagaga taaagaaatt aaaagagatg ggaaccgcgg
     2881 aacatcttct gcaacagctt atttactttc gtgcaaatat gaggaaagta aaagatgcag
     2941 gtcaaatcac ctctgacaga atggaaagcg gtatgaacac aaccctttac ataatcggca
     3001 catttttaac gccgacggac caaagtttcc agaagatctc ctcgttatcg gctcaattcc
     3061 ttatcaaggc ggttgtccct tatctggaaa ccgaagaccc gaacctgaag aaagaattgt
     3121 accgcatact taaaatagtg gacctggatg tcgcgggcaa gcaagtgcac cctaaataca
     3181 gtgagtatcc agtagattat accgagtaca agttgtttat ggaagttaat agagataatc
     3241 tatcggaacc gctggttcaa tatatgtatc aaaaatctcc cggagatgct ctgttgactc
     3301 tggcccaggt gtatctgaag gattccacag aaaaagattc cgttattcaa gcagtagcca
     3361 tcatcaggga ggatttttgg aagcgttatc acggcccggc ggaaagcaga ccgcgcataa
     3421 ttagcgttaa ggccatgcaa gctcttgatc aactttcggc gagatatccg cagtggtgga
     3481 tacagatgta cgttgtcgaa atcattcttc gtgaaccccg attcattaat ccgcaaattc
     3541 tcgagaggct caaaaatgct aagcaccctt ttatccttga aaagcttgaa aaaacatccg
     3601 gaaaataatc tttactgctt agagtgataa atttcgaatt aatttaaaat taatttaagg
     3661 gtaatttaag aggtcaaatc tttacatttg acaaaccggc aatgcggccg gttaaatctc
     3721 tggcagggca caatcttaaa agcgtttttg acgcaggtcg attgaggtcg tagcttttct
     3781 tccccgaggt tctatccctt cccttcaaaa gcacccgccc ggtttaatct ggatgggtat
     3841 tgttttttta ttggtcagtc caaccggccc aggaagtaaa gaccggcccc cagcaggcag
     3901 aggtcgggca ggaagaagag ggcggccagg ccggtcttca ggccggccag ggcctggagg
     3961 ccggacatgg cgaagtaata gccgagcacc aggccgcagg cggccgccac cccccagagg
     4021 tttttatgca cgacgatgcc cagcgggatg ccgatcaaaa taaagagcag cggggtgaag
     4081 gcgaaggcgg tccggcgcat gatttcaacc cgggcggccg gggagccgag ccgggccagc
     4141 ccggccagtg tctcctcgat gagcttgcgt ttcggcgcct tgaacccggg caggttccgg
     4201 ggcagcggga agagataggt gctgaacgag agtttctgga aggtttccgg cttctcgggc
     4261 tggtaggtct ggatgacgcc gtcctccagc atcaggttga gctttcccca gcgccagacc
     4321 ggccgcagcc ggcgggccga gaaggtgcgg acctggtccc ggtcgcgctg gtaaatggtc
     4381 acccgggcct ggctgtcctt gcggggaatg aagatgacga tgtccgggaa ctcggtcacc
     4441 accgagcccg gcttgatcag cgaggtctgg ggattgaagc ggctccgttc cagcagcagc
     4501 ttgccctggt agagggcgtt gggttcgatg acgccggtca ggccgagatg gccggccgag
     4561 aggagcaggc cgatcatcag cagcggcgcg gcaatccggc ccaccgaaat gccggcgccc
     4621 ttcagggcca ggtactcccg gtcctgggcc agccggctga agatcagcgt caccgcgatc
     4681 agcaccgcca gcgagaaggc gtagggcagc agggccagca gctgccagaa ccagtattcc
     4741 aggacctgga aggccggcag gatcttcttg accagcagtt cgaccagccg gaacaactgg
     4801 ccgagcgaga gcaggaagag cacgaagccc agcgccggca gtccggtggt ctggaattcc
     4861 ttcagcaggt agcggctgac gagtttcatg gtcgggccgg gattacgccc gggcgaaaga
     4921 tattgtagca catggaagcg gccgggcttg accggctcgg tctcagatgt tatcattctt
     4981 aaaaacgtaa cactgagacg gaggcgcgct cgtggagaag ataatccggt tcggagtttc
     5041 actgccggcg gagctgctga agcatttcga ccgggaatcc ggccgccgcc gctacgccaa
     5101 ccgatcggag gccatccggg acctgatgcg cgactggctg gtgcggcggg cctggtccga
     5161 ttcccggagc gaggtggtgg gcccgggcac cctggtt
//
LOCUS       DAOQYN010000140         5174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_16331_length_5174_cov_5.030084, whole genome shotgun sequence.
ACCESSION   DAOQYN010000140 DAOQYN010000000
VERSION     DAOQYN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16331_length_5174_cov_5.030084"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2156)
                     /gene="carB"
                     /locus_tag="PKN80_06310"
     CDS             complement(<1..2156)
                     /gene="carB"
                     /locus_tag="PKN80_06310"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNQ35662.1"
                     /translation="MGKRTDIRTLLIIGSGPIIIGQACEFDYSGSQACKALKEEGYRV
                     VLVNSNPATIMTDPGLADATYVEPLSIEFLTRVIEKERPDALLPTLGGQTGLNLAVGL
                     AEQGVLKAYGVECIGATPEAIARAEDRRLFKEAMEKAGLDLPRSGWAHDRTEAEAIAG
                     RLGFPLVIRPSFTLGGTGGGIAYNRDEFENLVGQGLQLSLIKEVLIEESVAGWKEFEL
                     EVMRDRRDNVVIICSIENFDPMGVHTGDSLTVAPAQTLTDREYQVMRDAAITCIRAIG
                     VDTGGSNIQFALDPATSRMVIIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     ISNDITRQTPACFEPAIDYCVVKIPRFTFEKFPGAEPLLTTAMKSVGEVMAIGRTFKE
                     ALGKGLRSMEIGAAGLGGFGPGLPEKPGLLERLRQPSPDRLFYLYRGLAAGIISMEEA
                     YQASGVDPWFLANLLEVAEAEAKLQSVAALDPEGMRAAKRLGFSDRRLAALTGRTEEQ
                     VRELRHRQGLRPAYKLVDTCAAEFAAATPYFYSTYEPGDEVAGRPASGRGKVIILGAG
                     PNRIGQGIEFDYCCVHASFALREAGYESVMVNCNPETVSTDYDTSDRLYFEPLTLEDV
                     LEVVAEERPLGVIVQFGGQTPLKLALDLERNRVPILGTRPSDIDRAEDRELFKAMLEK
                     LGLNQPPSGLARSLEQARAVAREIGYPILVRPSYVLG"
     gene            complement(2227..3549)
                     /gene="lpxC"
                     /locus_tag="PKN80_06315"
     CDS             complement(2227..3549)
                     /gene="lpxC"
                     /locus_tag="PKN80_06315"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNQ35663.1"
                     /translation="MANQATIKKPVSFAGEGLHTAHRSRVTFKPAQPDTGIVFKRIDL
                     PGQPEVRAVIANLLPEDRRSRRTSVGRDGVEIHTIEHFLATVLGMGVDNLLVEIDNDE
                     FPGEDGSSTRFAGLLLEAGLEEQSKARRAFVLRDPVFVNSNGSQLVALPSPTFRISYT
                     LDYPDPLGSQFGSFGIDRETFLSEIAPARTFCLQSEVDALLKTGLGKGSNYNNTLVID
                     QAGVVNNKLRFTNEMVRHKVLDLLGDLGLLGSVPQAHIIAIRSGHVLNVKLVSRMVEV
                     GRRETAAAVIPPDEPVDVHKQVLEAEDILKIIPHRPPFFFVDRILSIEEDRGIVGLKV
                     VSINEPYFAGHFPGHPVMPGVILIETMAQVAGVLMLRKPENRHKVAYFAAIDNARFRQ
                     AVLPGDQLRLEIEVVKLRSKVGQVHARALVNNGKLAAEADLMFALVER"
     gene            complement(3527..4570)
                     /gene="lpxD"
                     /locus_tag="PKN80_06320"
     CDS             complement(3527..4570)
                     /gene="lpxD"
                     /locus_tag="PKN80_06320"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNQ35664.1"
                     /translation="MQITVKELAKLVGGRIIGDPDQVITGVSGIKEAEPGEITFVANR
                     RYYCLMEGARASAILVPPEITSGSQTLIQVENPSLAFARIVALFGPEPVTFPPGIHPT
                     AVIGERVRLGRDVSIQPYVVIEDNVSIGDRTVLCAGVYVGHYSRLGDDCLVYPHVSIR
                     ERVAIGNRVIIHGGAVIGGDGFGFATVRGIHEKIPQIGTVILEDDVEIGSCVTIDRAR
                     FDDTLIKRGVKIDNLVQIAHNVTIGENSIVVAQVGISGSTTIGRNVIIAGQAGVVGHI
                     EVGDNAVIAAKAGVTKDIAPGAKVSGFPAREHSKDMKLQAYIHKQPELWKKILELEKR
                     IEELEKNGQSGDH"
     gene            complement(4602..5144)
                     /locus_tag="PKN80_06325"
     CDS             complement(4602..5144)
                     /locus_tag="PKN80_06325"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNQ35665.1"
                     /translation="MRKTALTVLGAAIVLVLGAGCAAAQEARLGVVDFEKVFADYGKV
                     AESQKEFDVFRTSRQAEVEKKAQEAEKEIKPLQEKLDKQGKALSKEASDKIKADIDEK
                     RGAVLQLQRDLIAELQKKNRELVEMRVNEIKEAIGKLAREKGIGLVVIKEVAIYNAEA
                     LDITAQVLDALNKPAAAEKK"
BASE COUNT          861 a         1720 c         1641 g          952 t
ORIGIN      
        1 ccgaggacgt aggaggggcg gaccaggatc gggtagccga tttcccgggc gacggcccgg
       61 gcctgctcca gcgagcgggc caggccgctg ggcggctggt tgaggcccag cttctccagc
      121 atcgccttaa agagctcccg gtcttcggcc cggtcgatgt cggacggacg ggttcccagt
      181 atgggtaccc ggttccgctc caggtcgagc gccagcttca gcggggtctg gccgccgaac
      241 tggacgatta cccccagcgg ccgttcctcg gcgacgacct ccagcacgtc ttccagagtg
      301 agcggctcga agtagaggcg gtcggaggta tcgtaatcgg tggaaacggt ctccgggttg
      361 cagttaacca tgacgctctc atagccggcc tcgcgcaggg cgaaggaggc gtgcacgcag
      421 caatagtcga actcgatgcc ctgaccaatc cggttggggc cggcacccag gatgatgacc
      481 ttcccgcgtc cactggccgg ccggccggcc acctcgtcgc cgggctcgta ggtggagtaa
      541 aagtaaggcg tggccgcggc gaattcggcc gcgcaggtat caaccagctt gtaagccggg
      601 cgcaatccct gacggtgacg cagctcgcgc acctgctcct cggtccggcc ggtcagggcc
      661 gccagccggc ggtccgaaaa tccgagccgc ttggcggccc gcattccctc gggatcgagg
      721 gcggccaccg actgaagctt ggcctccgcc tcggctacct cgagcaggtt ggccaggaac
      781 cagggatcga cgccgctggc ctggtaggct tcctccatag aaataatgcc ggcggccagc
      841 cctcggtaaa ggtagaagag acggtccgga gacggctgcc gcaaccgctc cagcagcccc
      901 ggcttctccg gcaggccggg tccgaagccg cccagcccgg ccgcaccgat ctccatcgaa
      961 cgcaatcctt tcccgagggc ctccttgaag gtgcggccga tggccatgac ctcgcccacc
     1021 gatttcatcg cggtggttag caagggttcg gcgcccggga acttttcgaa ggtaaagcgg
     1081 gggatcttga ccacgcagta atcgatggcc ggctcaaagc aggccggcgt ctggcgggtg
     1141 atgtcgttgc tgatctcgtc cagggtgtag cccaccgcca gcttggcggc gatcttggcg
     1201 atcgggaaac cggtcgcctt ggaagccagg gccgagctcc ggctcacccg cggattcatc
     1261 tcgataataa ccatccggct ggtggccggg tcgagcgcaa actgtatgtt ggagccgccg
     1321 gtgtccaccc cgatggcccg gatgcaggtg atggccgcgt cgcgcatcac ctggtactcc
     1381 cggtcggtca gcgtctgggc cggcgccacg gtcaggctgt caccggtatg gacgcccatc
     1441 gggtcgaagt tctcgatcga gcagatgatg accacgttgt cgcgccggtc ccgcatcact
     1501 tccagctcga actccttcca gccggccacc gactcctcga tcaacacctc cttgatcagc
     1561 gaaagctgga ggccctggcc gaccaggttc tcgaactcgt cccggttgta agcgatgccg
     1621 ccgccggtcc cgccgagcgt aaagctggga cggatgacca gcggaaaacc aagccggccg
     1681 gcaatggcct cggcctcggt ccggtcgtga gcccagccgc tcctgggcag gtcgagcccg
     1741 gccttctcca tcgcctcctt gaagaggcgg cggtcctcgg cccgggcgat cgcttcgggc
     1801 gtggccccga tgcactcgac cccgtaagcc ttaagaaccc cctgctcggc cagcccgacg
     1861 gccaggttga ggccggtctg gccgcccagg gtgggcagca gggcgtccgg ccgctccttc
     1921 tcaatgaccc gggtcaggaa ctcgatcgaa aggggctcaa cataggtggc gtcggccagg
     1981 ccggggtcgg tcatgatggt ggccgggttc gaattgacca gcaccacccg gtagccctcc
     2041 tccttgagcg ccttgcaggc ctgggaaccc gaatagtcaa actcgcaggc ctggccgatg
     2101 atgatcggcc ccgacccgat gatcagcaag gtccggatat cggttctttt gcccacgtaa
     2161 agacctcaaa gggggaaaac attgcgccgg cggacgcccg gcggagcagg agggggggta
     2221 actgcctcag cgctcgacca gggcgaacat caggtccgcc tcggcagcca gcttcccgtt
     2281 gttgaccagg gcacgggcgt gcacctggcc caccttgctg cgcagcttga caacctcgat
     2341 ttccagccgg agctggtcac cgggcagtac cgcctgccgg aagcgcgcgt tgtcgatcgc
     2401 ggcaaaataa gcgaccttgt ggcggttctc cggcttgcgc agcatcagga ctccggccac
     2461 ctgggccatc gtctcgatca ggatcactcc gggcatcacc gggtggcccg ggaaatgacc
     2521 ggcaaaataa ggttcgttga tggagaccac cttgaggccg acgatgcccc ggtcctcttc
     2581 gatggaaaga atccggtcaa cgaagaagaa gggcggccgg tgtggaatga tcttaagaat
     2641 atcctcggcc tccagcacct gtttgtgaac atccaccggc tcgtcgggcg gaatgaccgc
     2701 ggccgccgtc tcccggcggc cgacctccac catccgggag accagcttga cgttcaggac
     2761 gtgtccgctc cggatggcga tgatgtgcgc ttggggaacc gagcccagca ggccgaggtc
     2821 accgagcagg tcaagcacct tgtggcggac catttcattg gtgaaacgca gcttgttgtt
     2881 gaccacgccg gcctggtcga tgaccagggt gttgttgtaa ttcgacccct tgcccagtcc
     2941 ggtcttcagc agggcatcca cctcgctctg caggcagaaa gtgcgggccg gggcgatttc
     3001 ggaaagaaag gtttcccggt cgatgccgaa ggagccgaac tggctgccca gcggatccgg
     3061 atagtccagg gtgtaggaga tccggaaggt cggcgacggc agcgcgacca gctggctgcc
     3121 gttgctgttg acgaagaccg gatcccggag cacgaaggcc cggcgggcct tggactgctc
     3181 ctccaggccg gcctccagca gcagaccggc aaaccgggta ctgctgccgt cttcacccgg
     3241 aaattcgtcg ttgtctatct cgaccagcag gttatcaact cccatcccca gcacggtggc
     3301 caggaagtgc tcgatggtgt ggatttcgac cccgtcgcgc cccaccgagg tccggcggct
     3361 gcgccggtcc tcgggcagga ggttggcgat gaccgccctg acctccggct gccccggcag
     3421 gtcaatccgc ttgaaaacga tcccggtatc cggctgggcc ggcttgaagg tcacccgcga
     3481 acggtgggcg gtatgcaacc cttctcccgc gaaggaaacc ggctttttaa tggtcgcctg
     3541 attggccatt tttctccagc tcttcaattc tcttttccag ctccaggatc ttcttccaga
     3601 gttccggctg cttgtgtatg taggcctgca gcttcatgtc cttgctgtgc tcccgggccg
     3661 ggaaaccgga gaccttggcc ccgggcgcga tatccttggt caccccggcc ttggcggcga
     3721 tcaccgcgtt gtcgccgacc tcgatgtggc cgaccacgcc ggcctggccg gcgatgatca
     3781 cgttgcggcc gatggtggtg ctgccggaga tgcccacctg ggccaccacg atgctgttct
     3841 cgccgatggt cacgttgtgg gcgatctgga ccaggttgtc gatcttgacc ccgcgcttga
     3901 tgagggtgtc gtcgaagcgg gcccggtcga tggtcacgca ggaaccgatc tcgacgtcat
     3961 cctcgaggat gaccgtcccg atctggggta tcttttcgtg gatgccgcgc acggtggcga
     4021 aaccgaagcc gtcgccgccg atcaccgcgc cgccgtggat gatcacccgg ttgccgatcg
     4081 cgacccgctc ccggattgac acgtgcgggt acaccaggca gtcgtcgccc agccgggaat
     4141 agtggccgac atagaccccg gcgcagagaa ccgtccggtc gccgatgctg acgttatcct
     4201 cgatcaccac gtagggctgg atggaaacgt cccggcccag ccggacccgt tcgccgatga
     4261 ccgcggtcgg gtgaatcccg ggcggaaagg tgaccggttc cgggccgaag agggccacga
     4321 tccgggcaaa ggccagcgac gggttctcca cctggatcag ggtctgactg ccgctggtta
     4381 tctccggcgg caccaggatg gccgaggccc gggccccttc catcaggcag tagtatctgc
     4441 ggttggccac aaaggtgatc tcgcccggct cggcctcctt gattccggag acgccggtta
     4501 tcacctggtc ggggtcgccg atgatccggc ccccgaccag cttggccaat tcctttacgg
     4561 taatctgcat gtgcccgtcc ttcctggccg caaccggccg tttacttctt ttcggcggcg
     4621 gcgggcttgt tcagggcgtc aagcacctgg gcggtgatgt cgagcgcctc ggcgttatag
     4681 atggcgactt ccttgatgac caccaggccg atccccttct cccgggccag cttgccgatg
     4741 gcctccttga tctcgttcac ccgcatctcg accagctcgc ggttcttctt ctggagctcg
     4801 gcgatcaggt ctcgctgaag ctggagcacc gcgccgcgct tctcgtcgat gtcggccttg
     4861 atcttgtccg aggcctcctt gctgagggcc ttgccctgct tgtccagctt ctcctggagc
     4921 ggcttgatct ccttctcagc ctcctgggcc ttcttctcca cctcggcctg gcggctggtc
     4981 cggaaaacgt caaactcctt ctgactctcg gccaccttgc cgtaatccgc aaagaccttt
     5041 tcgaaatcca ccacgcccag ccgagcctcc tgggccgccg cgcaacccgc gcccaggacc
     5101 agcacgattg ccgcccccag gaccgtcaac gccgtctttc tcattggtcc gcctccttgg
     5161 aaaaaaccgc acct
//
LOCUS       DAOQYN010000141         5017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_16984_length_5017_cov_4.316606, whole genome shotgun sequence.
ACCESSION   DAOQYN010000141 DAOQYN010000000
VERSION     DAOQYN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16984_length_5017_cov_4.316606"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PKN80_06330"
     CDS             complement(<1..693)
                     /locus_tag="PKN80_06330"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HNQ35666.1"
                     /translation="MKPVIQVALDFIDLARALQVAREAVQGGADWLEAGTPLIKSVGL
                     DSVRKLRELFPGKTIVADLKTFDAGRLEVEMAAKAGAGIVCVLGNAPDATLKECVLAG
                     RNYGARIMADLLGVESVEARAVELVGFGIDYLNVHLGIDEQMQGRQSFDLIRRLARRV
                     EAPLAAAGGLNTETAVQALKAGASIFIVGGAITKSPDAAAATRLFKKALKEKRPGNTE
                     LYRRVGPEGLLEV"
     gene            930..2195
                     /locus_tag="PKN80_06335"
     CDS             930..2195
                     /locus_tag="PKN80_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNQ35667.1"
                     /translation="MEIRERIESYLVERLVPFWLDRVADPDGGFLTYFDRNGRPTGQT
                     DKTLIQQTRTLFMLSLLERSGRGAGRAGKLAAAGLDFLLEKFWDKEQGGWFWITDRGG
                     RPLQSDKIVYGQSFAIYALSEYALAVGDRRAADYAVRTFDLLQKYAADTAFGGYREMF
                     RSDWSPRPGGDLGGDRKSFDVHMHLMEAFTSLCELTGREIHRRKLREVVRVLWERMLD
                     PASGTGIAQFELDFRPRPAISFRTVWGSDRDHGGRPRPLDNTSYGHNIEFFWLLLRSL
                     EALGEPAAPYRARLRRLVEHTRRYGLDERYGGLYVEGRTAGPARDRQKEFWQQAEALV
                     GFLDAYLLFGERPYWEAFRNIFDFVWTKVINHQAGEWYALLDRRGRVRWDYLGHAWKN
                     NYHTVRSMTESLKRLERIQGRPELAAGLE"
     gene            2205..3260
                     /locus_tag="PKN80_06340"
     CDS             2205..3260
                     /locus_tag="PKN80_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNQ35668.1"
                     /translation="MNPKLKTGPYLDPVRLGELLKRIGRVRVAVIGDLCLDIYWLADL
                     TRSELSRETPHFPLPVIEERFSPGGGANVAANLAALGAARVTVLGLVGRDWRAGRLRA
                     ELVRRAISTAGLCAGAGRVTPAYVKPVRRGSSGVEYEDPRLDFDNYAPPTAAEEARLI
                     RRLERTWERVDALCVSDQLRFGSITPGVRARLIELARSGRKPVVIDSRDRIAGFTGPA
                     ILKPNELEAFRALSGAGRKPSLDPADRAGLALGFSALQKARVFMTAGAEGAFFAADGR
                     VEHLPAVPAAPPLDIVGAGDAFLAALTLALAAGASGAEAAQLGNLAAAVVIRKLKTTG
                     TASPAELLRKLTSMEKP"
     gene            3257..4090
                     /locus_tag="PKN80_06345"
     CDS             3257..4090
                     /locus_tag="PKN80_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35669.1"
                     /translation="MNQPDLAPLEILQELPPDRPALKFAVLDFDGTISTLRQGWEGIM
                     RPLMLEMIAGPTPVDPDLEREVDEFIDHSTGIQTIFQMQWLAEAVKRHGRNPDYHDDP
                     WWYKAEYNRRLMELVDRRIADIQSGRKAPSDYQIKGGLDFLAALQARRISIYFFSGTD
                     HGDVRRESTLIGAAAFAALIVGAPEGRAECSKEAVLRQLIQERGFKGPEVLVVGDGRV
                     EIALGREVGALTLGMATDEVRREGVNPRKRARLEKAGAHAIAGDFTCLPKLLAWLGLS
                     S"
     gene            4115..>5017
                     /locus_tag="PKN80_06350"
     CDS             4115..>5017
                     /locus_tag="PKN80_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35670.1"
                     /translation="MTFFERKIWSTLRQVQELLLEPGPEIKSGLMRRAFYLAPGRYRY
                     LDRTGFTGSFPFFARAGETLFFRAGLTIPRPPRNRRLLLQAGFYNAEGLLSLDGKPWV
                     GLDRNHKLVDLPAGWRPGSRHELEIEFQFISRPDWTYNPDQARTFLGLRLVFVSPEAL
                     DYHENLKAVNAAWEAAESGEKERLAAVLEESFRSLEIDPESPDFLVGANRILRRFLEG
                     GRAEPGFEVGLLGHSHLDTAYLWPMRETIRKCGRTFSTALRLMESDSDFIFGHSQPVQ
                     YRFCRDHYPELYRQVKARVAEGRWE"
BASE COUNT          780 a         1675 c         1679 g          883 t
ORIGIN      
        1 gacctcgagc agcccttccg ggccgacccg gcggtagagt tcggtgttgc ccggccgttt
       61 ctccttgagg gcctttttga aaaggcgggt ggcggcggcc gcgtcgggac tcttggtgat
      121 ggccccgccg acgatgaaga tggaggcgcc cgccttcagc gcctggaccg cggtctcggt
      181 gttgaggccg ccggcggcgg ccagcggcgc ctcgacccgg cgcgcgagtc ttcggatcag
      241 gtcgaaggat tggcgcccct gcatctgctc gtcgatgccc aggtgaacat tgagatagtc
      301 gattccgaaa ccgaccagtt cgacggcccg cgcctcgacc gattcaacgc cgagcaggtc
      361 ggccatgatg cgggccccgt agttgcgtcc ggccaggacg cactccttca aggtggcgtc
      421 cggcgcgttg ccgagcacgc agacgatgcc ggccccggcc ttggccgcca tctccacctc
      481 cagccggccg gcgtcgaagg tcttcaggtc ggccacgatg gtcttgcccg gaaagagttc
      541 gcgcaacttg cggaccgagt ccaggccgac gctcttgatg agcggcgtgc cggcctccag
      601 ccagtcggcg ccgccctgga ccgcctcccg ggcgacctgg agagcccggg ccaggtcgat
      661 gaaatcaagt gccacctgaa tgaccggttt catctgtttt tgcctccgga attccttgaa
      721 gaattattga atgccgcggg ttcctttaat ttgaaatatg ataccacaac cggccggcgc
      781 ctgtctttgt ccggcccggt tccgcctcgc ctcgggacgg gacggaatcg gttgcttttt
      841 tgaacccttt tcggttactt tatatagatc gtggaaaggg tggaaccacc ggcctggcga
      901 tacctggaac ccccatccgg agcggtgcga tggaaattcg ggaaaggatt gaatcttacc
      961 tggtcgagcg cctggttccc ttctggctgg accgggtggc cgatccggac ggcggctttc
     1021 tcacctattt tgatcgaaac ggccggccga ccggccagac cgacaagacc ctgatccagc
     1081 agacccgcac cctctttatg ctgtccctgc tggagcggag cggccggggc gccggccggg
     1141 ccggaaaact ggcggccgcc gggctggatt tcctgctgga aaagttctgg gacaaggagc
     1201 agggcggttg gttctggata accgaccggg gcggccggcc cctccagtcc gacaagatcg
     1261 tttacgggca gagtttcgcc atctacgccc tgagcgagta tgctctggcc gtcggcgacc
     1321 ggcgcgcggc cgattatgcg gtccggacct tcgacctgct ccagaagtac gcggccgaca
     1381 ccgccttcgg cggctaccgg gaaatgttcc ggtccgattg gagcccgcgg cccggcggcg
     1441 acctgggcgg ggaccggaag tccttcgacg tccacatgca cctgatggag gcctttacca
     1501 gcctctgcga gctgaccggc cgggagattc atcgccgcaa gctccgcgag gtggtccggg
     1561 tgctctggga gcgcatgctc gatccggcca gcggtaccgg gatcgcccag ttcgagctcg
     1621 atttccggcc ccggccggcc atcagcttcc gcaccgtctg gggttccgac cgggaccacg
     1681 gcggccggcc ccggcccctg gacaacacca gctacggcca caacattgag ttcttctggc
     1741 ttctgctgcg cagcctggaa gcgctcgggg aaccggcggc cccctaccgg gcccggctgc
     1801 gccgcctggt ggagcacacc cggcgttacg ggctggacga gcggtacggc ggactctacg
     1861 tggaggggcg gacggccggg ccggcgcggg accggcagaa ggagttctgg cagcaggccg
     1921 aggcgctggt cggtttcctg gacgcctacc ttctcttcgg ggaacggcct tactgggagg
     1981 ccttccggaa cattttcgat tttgtctgga cgaaggtcat caaccaccag gccggcgagt
     2041 ggtacgccct gctggaccgc cgaggccggg tccgctggga ttacctcggc cacgcctgga
     2101 agaataatta ccacacggtg cgttccatga ccgagagctt gaagaggctg gagcgcatac
     2161 agggccggcc ggaactggcg gccgggctgg agtgaggaat ccgcatgaat ccgaaactta
     2221 aaaccggtcc ttacctggac ccggtccggc tcggggaatt gctgaagcgg attggccggg
     2281 tacgggtggc ggtgatcggc gacctctgcc tcgacattta ctggctggcc gatcttaccc
     2341 ggagcgaact ctcgcgggag acgccccatt tcccgctgcc ggtgattgag gagcgttttt
     2401 cgcccggagg cggcgccaac gtggccgcca acctggccgc gctcggcgcg gcccgggtga
     2461 ccgtcctggg cctggtcggc cgcgattggc gggccggtcg gctgcgggcg gaactggttc
     2521 gccgcgccat ttcgaccgcc ggcctctgcg ccggagcggg tcgggttacg cccgcctacg
     2581 tcaagccggt ccgccggggc agctcggggg tcgagtatga ggatccccgg ctcgatttcg
     2641 acaattacgc gccgccaacc gccgccgagg aggccaggct gatccggcgg ctcgaacgca
     2701 cctgggaacg ggtggacgcc ctctgcgtct ccgaccagtt gcgtttcggt tcgatcacgc
     2761 ccggagtgcg ggcccggctg atcgagctgg cccgttccgg ccgcaagccg gtggtgattg
     2821 acagccgcga ccggatcgcc gggttcaccg gtccggccat tctgaagccg aatgaactgg
     2881 aggccttccg ggccctctcc ggcgccggcc ggaaaccgag cctcgatccg gctgaccggg
     2941 ccggcctggc tctcggattt tcggcccttc agaaggctcg ggtcttcatg accgccggtg
     3001 ccgagggcgc cttttttgcc gccgacggcc gggtggagca cctgccggcg gttccggcgg
     3061 cgccgcccct ggacatcgtg ggagccgggg atgccttcct ggcggcgctg accctggcgc
     3121 tggccgccgg cgcctccgga gcggaggcgg cccaattggg gaacctggcc gccgcggtgg
     3181 taatcaggaa actcaagacg accggcaccg cctccccggc ggagctgctc cggaaactaa
     3241 cctcaatgga gaagccatga accaacccga cctggcgccg cttgaaatac tgcaggaact
     3301 gccgccggac cggccggccc tgaagttcgc cgtcctggat tttgacggca ccatctccac
     3361 ccttcgccag ggctgggagg gcatcatgcg tccgctgatg ctggagatga tcgccggccc
     3421 gacgccggtc gatccggacc tggaacggga ggtggacgag ttcatcgacc attcgaccgg
     3481 tatccagacc atcttccaga tgcagtggct ggccgaggcg gtgaaacgcc acggccggaa
     3541 ccccgattac cacgacgacc cctggtggta caaggccgag tacaaccgcc ggctgatgga
     3601 actggtcgac cgccggatcg ccgatatcca gtccggccgc aaggctccct ccgattacca
     3661 gatcaagggc ggcctcgatt tccttgcggc cctgcaggcg cgccggatct cgatctattt
     3721 cttcagcggc accgatcacg gcgatgtccg gcgcgagtcg accctgatcg gcgcggccgc
     3781 cttcgcggcc ttgatcgtcg gcgccccgga gggacgggcc gagtgctcca aggaggcggt
     3841 tctgcgccag ctgatccagg agcgcggttt caaggggccg gaggtgctgg tggtcggcga
     3901 cggccgggtt gagatcgccc tgggtcgcga ggtgggcgcc ctgaccctgg gtatggccac
     3961 cgacgaggtc cgccgggagg gcgtcaaccc gcgcaagcgg gcgcggctgg agaaggccgg
     4021 ggcccacgcc atcgccggcg atttcacctg tcttccgaaa ctcctggcct ggctgggcct
     4081 ttcatcttga atccctctta acaaaggcgg cgcgatgact ttcttcgaac gaaaaatctg
     4141 gtcgacgctc cggcaggtcc aggaattgct cctggaaccg ggtcccgaaa tcaagtccgg
     4201 cctcatgcgc cgggccttct acctggctcc cggccgttac cgttacctgg accggaccgg
     4261 attcaccggg agttttccct tcttcgcccg cgccggcgaa acgctcttct tccgggccgg
     4321 gctgacgatt ccccgtccgc cccggaaccg gcgtctgctc ctgcaggccg gattttataa
     4381 cgccgagggg ctgctctccc tggacgggaa accctgggtc ggccttgacc gcaaccacaa
     4441 gctggtggac cttcccgccg gctggcggcc cgggtcgcgc cacgaactgg agatcgaatt
     4501 ccagttcatc tcccggcccg attggaccta caacccggac caggcgcgca cctttctcgg
     4561 gttacggctg gtcttcgttt cgcccgaggc gcttgattac cacgaaaacc tgaaggccgt
     4621 caacgccgcc tgggaggcgg ccgaatccgg cgagaaggaa cggctggcgg cggtgctgga
     4681 ggagtccttt cgctccctgg aaatcgaccc ggaatcgccc gactttctcg tcggcgccaa
     4741 ccggatcctg cgccgtttcc tggagggcgg ccgggccgag cccggtttcg aggtcggtct
     4801 gctgggccac agccacctgg ataccgccta cctctggccg atgcgcgaaa cgatccgcaa
     4861 gtgcggccgg accttctcca ccgccctgcg gctgatggag tcggattccg atttcatctt
     4921 cggccactcc cagccggtcc agtaccgctt ctgccgcgat cattacccgg aactctaccg
     4981 gcaggtcaag gcccgggtcg ccgaggggcg ctgggag
//
LOCUS       DAOQYN010000142         5008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_17025_length_5008_cov_4.284474, whole genome shotgun sequence.
ACCESSION   DAOQYN010000142 DAOQYN010000000
VERSION     DAOQYN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17025_length_5008_cov_4.284474"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PKN80_06355"
     CDS             complement(<1..957)
                     /locus_tag="PKN80_06355"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNQ35671.1"
                     /translation="MAKYIFVTGGVLSSLGKGVASASIGTLLEARRYRINMQKFDPYL
                     NVDPGTLTPFQHGEVFVTRDGAETDLDLGHYERFTSLTLNRNSNTTSGQVYYEVIERE
                     RRGDFLGKTVQVVPHITEEIKRRFRRLARGNAEIVIAEIGGTVGDIESLPFLESIRQF
                     RQEVGAENICCIHLTYIPYIQAAGELKSKPTQHSVERLREIGIQPDIILCRTEKPLNQ
                     DLREKISLYCNVPFQAVIEARDTDCIYEIPLTFHQEKLDQVILRKLRLRPRVSHLKEW
                     GKLIDQLRNPEHEVEIAMVGKYVKLQDSYKSLDEALRHGGLAS"
     gene            complement(1082..1870)
                     /locus_tag="PKN80_06360"
     CDS             complement(1082..1870)
                     /locus_tag="PKN80_06360"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNQ35672.1"
                     /translation="MTGLERATQMIGSKAVRLPLLLFCLLLLVRPAAATPRIFWEKTG
                     EKRIFEGGAQKIRHLFRFRNEGDSQLRIERVRRTCGSCSVFNLSRKTLAPGAEAQLEI
                     EVAFGPGETVKKTYVYIESNDPANPFFQIALESEAPGINYPAQVFFENVPAHTPAARS
                     IEIFAVGERGFGIREIRTGPLVESVRADTIKENRRYRLEIRLKPRPPVKFGQDYVKIR
                     TDYPGLEEIEIRLYASMVSSAGGVSGEGRPRDPEAPDPKRDRRR"
     gene            complement(1886..2653)
                     /locus_tag="PKN80_06365"
     CDS             complement(1886..2653)
                     /locus_tag="PKN80_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNQ35673.1"
                     /translation="MKLGIVTYQIAAEWDLDRIISACGSLGYAGVELRTTHRHGVEPS
                     LSKAARAEVKKKFEDSPVVLVGLGSAFEYHSPDPAELKKNLDGTREFVLLARDTGAEG
                     VKVRPNGFPEGVPKEKTLEQIGLSLRESASFAADHGVKIRLEVHGSGTSHPPCIRRIV
                     DIAGHPNLYVCWNSNQADRDETGSIEKHFDLLKDRLEICHINELCNDYPWRTLFSLLR
                     KNGFDGYCLAEVAASPEPERFLKYYKSLFEALTRPLN"
     gene            complement(2698..3414)
                     /gene="lptB"
                     /locus_tag="PKN80_06370"
     CDS             complement(2698..3414)
                     /gene="lptB"
                     /locus_tag="PKN80_06370"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNQ35674.1"
                     /translation="MLAAEKLYRNYRGKPAVADLSLQVERGEIVGLLGPNGAGKTTTF
                     YLLMGWLRPDGGRVCLNGTDITGLPMYRRARAGLGYLPQEPSVFGKLTVAENLLAVLE
                     FRRLPGAERRERCAAALEKLNLTGLTGRVAGTLSGGERRRVEVARSLVMDPAFLLLDE
                     PFTGIDPLNIEELRGIIRGLARDGIGVLITDHNVRETLAITDRSYLIFQGRVLISGSG
                     RELVDSPEARRLYLGESFSL"
     gene            complement(3421..4458)
                     /gene="ruvB"
                     /locus_tag="PKN80_06375"
     CDS             complement(3421..4458)
                     /gene="ruvB"
                     /locus_tag="PKN80_06375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNQ35675.1"
                     /translation="MAKEREELIRPETLPDDLRYDIALRPERFDDFIGQEKVKENLGV
                     FITATLKRRDPLDHTLFSGPPGLGKTTLAHIISQELGVKIKATTGPAIERPGDLAGLL
                     TNLGRSDVLFIDEIHRLNRTVEEYLYGAMEDFHIDIVTGDGPRANSIRLQIEPFTLIG
                     ATTRSGLLSAPLRSRFGITLRLDYYQAAELARIVQRSARILRVEVSNEAAAEIARRSR
                     GTPRIANRLLRRVRDFAQVDGAPSIQADLAMQALDRLDVDRHGLDEMDKRILEVIISK
                     FSGGPVGIKTLAVAVGEEPDTLEEVYEPFLVREGYLERTLKGRRSTLRAYQHLGLEPP
                     PAAGDGQNRLL"
     gene            complement(4511..>5008)
                     /locus_tag="PKN80_06380"
     CDS             complement(4511..>5008)
                     /locus_tag="PKN80_06380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HNQ35676.1"
                     /translation="PLSTYDRLPETGEEIFLYVQAAYREDSVTLYGFAGREEKTLFNL
                     LLGVSGVGPKVALAILSGTTVPGFCNAIIAQDDKVLSAISGVGKKLSQRLITELKDRV
                     ASLGDLVAARPGSTINPEAVNALVALGYSRVQAVRAVQAVQGEAKTGDLETLIRRVLK
                     RISSG"
BASE COUNT          822 a         1577 c         1692 g          917 t
ORIGIN      
        1 gctggccagg ccgccgtggc gcagggcctc gtcgagggat ttataggaat cctggagctt
       61 gacgtacttg cccaccatgg cgatctccac ctcgtgctcc gggttgcgca gctggtcgat
      121 cagcttcccc cactctttaa ggtgggagac ccgcgggcga agccgcagct tgcgcaggat
      181 cacctgatcg agcttctcct ggtggaaggt gagcgggatc tcgtagatgc agtcggtatc
      241 gcgggcctcg atcaccgcct ggaagggcac gttgcagtaa aggctgatct tctcgcgcag
      301 gtcctggttc aggggcttct cggtccggca gaggatgatg tccggctgga tgccgatttc
      361 gcgcaggcgc tccaccgagt gctgggtagg cttgctcttc agttcgccgg cggcctggat
      421 gtaggggatg taggtgaggt ggatgcagca gatgttctca gcccccacct cctggcggaa
      481 ctggcggatg gattccagaa aaggcaggct ctcgatgtcg cccaccgtgc cgccgatttc
      541 ggcgatcacg atttcggcgt tgccgcgggc caggcggcgg aaacgccgct tgatctcctc
      601 ggtgatgtgc ggaaccacct ggacggtctt gcccaaaaaa tcgccgcgcc gctcccgctc
      661 gatcacctcg tagtaaacct gaccggaggt ggtgttggaa ttccggttca gggtcaggct
      721 ggtgaagcgc tcgtagtggc ccaggtccag gtcggtctcg gccccgtcgc gggtgacgaa
      781 gacctcgccg tgctggaagg gcgtgagagt gccgggatcg acgttgaggt agggatcgaa
      841 cttctgcatg ttgatgcggt agcggcgggc ttccaggagg gtgccgatcg aagcggaggc
      901 gacgcctttt cccagcgagg aaagtacgcc gccggtcaca aagatatact tggccatgag
      961 ggatcccgga taagggcgaa aaactgcttg aaaaaatgga ggttgttata aatttaccac
     1021 accggccggc ttccgtcaaa agagggattg cccggcgccg ccggccgttg aaaacggaac
     1081 ttcaccggcg ccgatcccgt ttcgggtccg gggcctccgg gtcccgcggc cggccctccc
     1141 cggagacacc gccggccgaa gagaccatgc tcgcgtagag gcggatttca atctcctcga
     1201 ggccgggata atcagtgcgg atctttacgt aatcctggcc gaatttcacc ggcggccgcg
     1261 gtttcaagcg gatctccaac cggtacctcc ggttctcctt gatcgtgtcg gcccggacgc
     1321 tctcgaccag ggggccggtc cggatttccc ggatcccgaa accccgttcc ccaacggcga
     1381 agatctcgat gctcctggcc gcgggggtat gcgcggggac gttttcgaaa aagacctggg
     1441 cgggataatt gatgcccggc gcctcacttt cgagggcgat ctggaaaaac gggttggccg
     1501 ggtcgttgct ctcgatataa acgtaggtct tcttgacggt ctcaccgggc ccgaaggcca
     1561 cctcgatctc cagctgcgcc tcggcgcccg gcgcgagcgt tttccgggaa aggttgaaaa
     1621 cggaacagct tccgcaggtc cggcggaccc gttcgattct caactgggag tccccctcgt
     1681 tgcggaaccg gaaaagatgc ctgatcttct gcgccccgcc ctcgaagatc cgcttctccc
     1741 cggtcttctc ccagaagatc cggggcgtgg cggccgccgg ccggaccagg agaagcaggc
     1801 aaaagagcag cagcggcagc cggacagcct tcgaaccaat catctgggtt gccctttcca
     1861 ggccggtcat catctccggc cggcttcagt tcaacggccg ggtcaaggcc tcgaagagac
     1921 ttttgtagta tttcaggaag cgctccggct ccgggctggc ggccacttcg gccaggcagt
     1981 atccgtcgaa gccattcttc cgcaggaggg agaagagcgt ccgccagggg taatcgttgc
     2041 agagttcgtt gatgtggcag atctcaaggc gatccttcag gaggtcgaag tgcttttcaa
     2101 tcgagccggt ttcgtcccgg tcggcctggt tggaattcca gcagacgtaa agattcgggt
     2161 ggccggcgat gtccactatc cgcctgatac agggcggatg agaagtgccg gaaccgtgga
     2221 cttccagtcg gattttcacc ccgtggtcgg cggcaaagga ggcggactcc cgcaaagaaa
     2281 gaccgatctg ctccagcgtc ttttccttcg gcacgccctc gggaaaaccg ttgggcctga
     2341 cttttactcc ttccgcgccg gtgtcccggg ccagcagaac gaactcccgg gtcccgtcca
     2401 ggttcttctt cagttccgcc ggatccgggc tgtgatactc gaaggcgctg cccagcccga
     2461 ccagcaccac cggcgaatcc tcgaactttt ttttcacctc ggcccgggcc gccttcgaga
     2521 gcgagggttc cacgccgtgc cggtgcgtcg tgcgcagttc caccccggcg tagcccagcg
     2581 aaccgcaggc cgagatgatc cggtccaggt cccactcggc cgcgatctgg taggtgacga
     2641 taccaagttt catgaaaggc tcccggttga aaaaatcgga tccggccggg ccgcgtctca
     2701 gaggctgaag ctctcgccca ggtacaggcg ccgcgcctcc gggctgtcga ccagttcccg
     2761 cccggaaccg gagatcagca cccgcccctg gaagatcagg taggagcggt cggtgatggc
     2821 cagtgtttcg cgcacgttgt ggtcggtgat cagcaccccg atcccgtcgc gggccaggcc
     2881 gcggatgatg ccgcgcagct cctcgatgtt cagggggtcg atcccggtga agggctcgtc
     2941 gagcagcagg aaggccgggt ccatcaccag gctccgggcc acctcgaccc ggcgccgctc
     3001 gccgccggag agtgtcccgg ccacccggcc ggtcaggccg gtcaggttga gcttctccag
     3061 ggcggccgcg cagcgctccc gccgctcggc ccccgggagg cggcggaact cgagcaccgc
     3121 cagcaggttc tcggcgaccg tcagcttgcc gaagaccgac ggctcctggg gcaggtagcc
     3181 caggccggcc cgggcccgcc ggtacatcgg caggccggtg atgtccgtcc cgttgaggca
     3241 aacccggccg ccgtcgggac gcagccagcc catcagcagg taaaaggtgg tcgtcttgcc
     3301 ggcgccgttg ggtccgagca ggccgacgat ctcgccccgc tccacctgga gcgagagatc
     3361 ggcgaccgcg ggtttgccgc ggtagttccg gtagagtttt tcggcggcca gcatggggat
     3421 tcaaagcagc cggttctgtc cgtctccggc ggccggcggc ggttcgagac cgagatgctg
     3481 ataggcgcgc agcgtgctcc ggcgcccctt gagcgtccgt tccaggtatc cctcgcgcac
     3541 caggaagggt tcgtagacct cctccagggt gtccggctcc tcgcccacgg ccaccgccag
     3601 ggtcttgatg ccgaccgggc cgcccgaaaa cttgctgatg atgacctcca ggatgcgctt
     3661 gtccatttcg tccagcccgt ggcggtcgac gtcgagccgg tccagggcct gcatggccag
     3721 gtcggcctgg atcgagggcg ccccgtccac ctgggcgaaa tcgcgcaccc ggcgcaggag
     3781 gcggttggcg atgcgcggcg tgccgcggct gcggcgggcg atttcggcgg cggcttcgtt
     3841 ggacacttcg acccggagga tccgggccga gcgctggacg atccgggcca gttcggcggc
     3901 ctggtagtag tcgaggcgga gggtgatgcc gaagcggctg cgcagcggcg ccgagagcaa
     3961 gccggagcgg gtggtggcgc cgatcagggt aaagggttcg atctggaggc ggatggagtt
     4021 ggcgcggggg ccgtcgccgg tgacgatgtc gatgtggaaa tcctccatgg ccccgtagag
     4081 gtactcctcg acggtccggt tgagccggtg gatctcatcg ataaagagca cgtcggaccg
     4141 gcccaggttg gtcagcaggc cggccaggtc gcccggccgc tcgatggccg ggccggtggt
     4201 ggccttgatc ttgacgccca gttcctggga gatgatgtgg gccagggtcg tcttgcccag
     4261 gccgggcggc ccggagaaga gcgtgtggtc gagcgggtcc cggcgtttca gggtggccgt
     4321 gatgaagacc ccgagattct ccttgacctt ctcctggccg atgaagtcgt caaagcgctc
     4381 cggccggagg gcgatgtcgt agcgcaggtc gtcgggcagc gtctcgggtc ggatcagttc
     4441 ttcccgttct ttggccatct tatcggcacc tctgcctgga tctcgttatc ggccgccgcg
     4501 gccgtggttt tcaaccgctg gaaatgcgct tcaaaacccg cctgatcagg gtctccaggt
     4561 ccccggtctt cgcctcgccc tggacggcct ggacggcccg gaccgcctgc acccggctgt
     4621 agccgagcgc caccagggca ttgaccgcct ccgggttgat ggtcgacccg ggccgggcgg
     4681 cgaccaggtc gccgagggag gcgacccggt ccttcaactc ggttatcagg cgctggctga
     4741 gcttcttgcc gaccccggag atggcgctca gcaccttgtc gtcctgggcg atgatcgcgt
     4801 tgcagaagcc gggcaccgtg gtcccggaca ggatggccag cgccaccttc ggcccgaccc
     4861 cgctaacgcc cagcaggagg ttgaaaaggg tcttctcctc gcggccggcg aaaccgtaaa
     4921 gggttacact atcctcgcga taggcggcct ggacgtaaag aaaaatctct tcgccggtct
     4981 ccggcaggcg atcgtaagtc gagagggg
//
LOCUS       DAOQYN010000143         4970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_17216_length_4970_cov_4.583723, whole genome shotgun sequence.
ACCESSION   DAOQYN010000143 DAOQYN010000000
VERSION     DAOQYN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17216_length_4970_cov_4.583723"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PKN80_06385"
     CDS             <1..392
                     /locus_tag="PKN80_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ35677.1"
                     /translation="SLMAFLFVLKIEGGGLVRARSMAFFVLACSQLFHAYNCRHPLKS
                     IFQLGIFSNRNLVLAAGFSFLLLLAVIYLPPLAAIFKSEPLPALDLAVAVAFSSFPLW
                     GMELVKVLKRRKSGRSGKLSGEAVFQA"
     gene            complement(383..985)
                     /locus_tag="PKN80_06390"
     CDS             complement(383..985)
                     /locus_tag="PKN80_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNQ35678.1"
                     /translation="MYILAGRFKGRRLSVPANRILRPTTGLLRKALFDLLAPGLAGAD
                     FLDLYAGVGSVGLEALSRGAGRVTLVEGDGRVGESLRENIRILLEASGRDGGEPITPD
                     RVVFWPRPVEKSLARLGETGSRYRFIFADPPYRTDPKELAGLLLSIKKARLLEEGGRL
                     VLQHDQHLLKDPRFQKTLAAEANPPAIRRYGSSALTIFYA"
     gene            complement(1036..1938)
                     /gene="secF"
                     /locus_tag="PKN80_06395"
     CDS             complement(1036..1938)
                     /gene="secF"
                     /locus_tag="PKN80_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNQ35679.1"
                     /translation="MNFFPKTTTFDFMGKRGIFFIISLAITIIGLALTFIKGNRVLSM
                     DFTGGYLTQLEFSQEVDISQVRGILGERGYSDIILQKVGETGRRFIIKTKQGGHEIIQ
                     TLRENFPAVSISVLSEEQVSPTMGQTIRKQTLLACLLAICGILIYIAFRFEFRFAVAA
                     TIATIHDLLVTLAFVVVSGREIDSLVIAAFLTILGYSVNDTVVIFDRIRENLRGLTRV
                     ADPVEVFNRSLNETLSRTMITGVTSLFTLGCLYFLGGPVLNTFAFTLIIGIITGTYSS
                     IFIASAIVIAWEKRAPHRFRTSSS"
     gene            complement(1954..3276)
                     /gene="secD"
                     /locus_tag="PKN80_06400"
     CDS             complement(1954..3276)
                     /gene="secD"
                     /locus_tag="PKN80_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNQ35680.1"
                     /translation="MKGIYIRLFVILAVIGLSIWGFWPPQQKIRLGLDLRGGMQLVLQ
                     VRQPLVESKIPTTELTDRTLEVVRNRVDSLGLAEPVLQKIGQDRIMVQLPGVDDPDRA
                     MEILGKTAFLEFKMVEDNPELLKSAIAGTVPEGYELLYQYEKDAHGISKPSVPLLLKS
                     QAAVTGQDLSNAQLGYSQGALPVVDLEFNSEGARKFAEVTAANVNKRLAIVLDGIVQS
                     APVIREAIPSGKAQISGRFTTQEANDLALVLKAGALPARIEIIDRTVVGPSLGRDSIN
                     AGVKASLLGAILVGAFMIVYYSAGGLAAVFALGVNILILMAVMAYFRQTLTLPGIAGI
                     ALTIGMAVDANVLIFERIREELKAGRTIGAALDRGYDRALVAIIDSNLTTVIAAAILL
                     ALGRGSVKGFGLTLTIGVLANIFTAVFVTKVLFNLAISHFHIKKLHIG"
     gene            complement(3351..4883)
                     /gene="xylB"
                     /locus_tag="PKN80_06405"
     CDS             complement(3351..4883)
                     /gene="xylB"
                     /locus_tag="PKN80_06405"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HNQ35681.1"
                     /translation="MAADLFLGIDVGTSGVKVLLVDSNGRVVDHQDESYPLLEPRPLW
                     AEQDPEAWWRATARALRKLSERQPLKRRLAAVGLAGQMHGSVFLDGRDRVVRPALIWC
                     DQRTGPEAAEIERAVGSEKLRRITGNPLFTGFTAPKVRWLQKHEPANYRRTARLFLPK
                     DYIRYRLTGTAASEVTDNSGTGFFDVNRRNWSPEIMAAAGVPDRWLPPVLESPALAGQ
                     VSPEAARRTGLPAGCPVAAGAGDQAASGVGNGVVRPGVVSATLGTSGVVFAYSERIRR
                     DPAGRIHTMCHAVPGAWHLMGVTLAAGGALRWYRDAFCPAEKAEAQRRRIDPYEIMTA
                     AAGRIPAGADGLLFLPYLNGERTPHANPMARAVFFGATSRHTRAHFTRAVLEGVTFAL
                     RDSLEIMKDLKLPLREVRLSGGGARSALWCQMQADNFQALCRRTVNPDTTALGAAILA
                     ACGAGAFASVAEGCAGMVRTGRTFRPEPAAGRRYREAYRIFKALYPALAPYFDRAAGL
                     EV"
BASE COUNT          838 a         1558 c         1712 g          862 t
ORIGIN      
        1 gcagcctgat ggccttcctc tttgtcctca agatcgaagg cggcggactg gtgcgggcaa
       61 ggagcatggc cttcttcgtc ctggcctgct cccagctgtt ccatgcctac aattgccgcc
      121 acccgctcaa gtcgatcttc cagctgggga ttttctcgaa ccgcaacctg gtcctggcgg
      181 ccggcttctc cttcctgctg ctgctggccg tcatctacct gccgccgctg gccgccatct
      241 tcaagagcga accgctgccg gccctggacc tggcggtggc ggtcgccttc tcatccttcc
      301 cgctctgggg gatggaactg gtcaaggtct tgaagcgccg gaagtccggc cggtccggga
      361 agttgtccgg ggaagcggtt tttcaggcgt aaaagatggt cagggccgag gagccgtagc
      421 ggcggatcgc gggcggattt gcctccgcgg ccagggtctt ctggaagcgg gggtccttga
      481 gcaggtgctg gtcatgctgg aggacgaggc ggccgccttc ttccagcagc cgcgcttttt
      541 tgatggagag aagcaggccg gccagctcct tcggatcggt ccggtaaggc ggatcggcga
      601 agatgaaacg gtagcggctg cccgtttcgc cgaggcgggc caggcttttt tccaccggcc
      661 ggggccagaa aacgacccgg tccggcgtga tcggttcgcc gccgtcgcgg ccggaagctt
      721 ccagcaggat ccggatgttc tcccgcagcg actcgcccac ccggccatcc ccctccacca
      781 gcgtgaccct tccggcaccc cggctcaggg cctccaggcc gaccgagccc accccggcat
      841 agagatcgag gaagtcggcg ccggccagtc cgggcgccag gaggtcaaag agggccttgc
      901 gaagcagccc ggtggtcggc cgcaggatcc ggttggccgg caccgagagg cggcgtccct
      961 tgaaacggcc ggccagtatg tacatcggag tggctccgct ggtaccggcg ccccaatcgg
     1021 ctgggcggcg cttttttagg aggaggaggt ccggaaacgg tggggggccc ttttctccca
     1081 ggcgatgacg atggccgagg cgatgaagat cgaggagtag gtgccggtga tgatgccgat
     1141 gatcagagtg aaggcgaagg tgttgagcac cgggccgccc aggaagtaga ggcagccgag
     1201 ggtgaagagc gaggtgaccc cggtgatcat tgtcctggac agggtttcat taagcgaccg
     1261 gttgaagacc tcgaccgggt cggcgacccg ggtaaggccg cgcaggttct cccggatccg
     1321 gtcgaagatg accaccgtgt cgttgaccga gtaaccgagg atggtcagga aggcggcgat
     1381 caccaggctg tctatttccc ggccgctgac gaccacgaag gccagcgtga ccagcaggtc
     1441 gtggatggtg gcgatggtcg cggccaccgc gaagcgaaat tcgaaacgga aggcgatgta
     1501 gatcaggatg ccgcagatgg ccagcagaca ggccagcagg gtctgcttgc ggatcgtctg
     1561 gcccatggtc ggactgacct gctcctcgct caagacgctg atcgagaccg ccgggaagtt
     1621 ctcccggagc gtctggatga tttcgtggcc gccctgcttg gtcttgatga tgaagcggcg
     1681 gccggtttca cccaccttct ggaggatgat gtcgctgtag ccgcgttcgc ccaggatgcc
     1741 ccgtacctgg ctgatatcaa cttcctggct gaattccagc tgggtcaggt aaccgccggt
     1801 gaagtccatc gagagaaccc ggttgccctt gataaaggtc agggccaggc cgataatcgt
     1861 tatcgccagg gagatgatga agaatatccc ccgcttgccc atgaaatcga aagtggtcgt
     1921 tttcggaaaa aagttcatgg ccggattcct ttattacccg atatgaagtt tcttgatatg
     1981 gaagtgggag atggccaggt tgaagagcac cttggtcacg aagacggcgg tgaagatgtt
     2041 ggccagcacc ccgatggtca gcgtcagtcc gaagcccttg accgatccgc ggcccagggc
     2101 cagcaggatg gcggccgcga tcaccgtggt caggttggag tcgatgatcg ccaccagggc
     2161 ccggtcgtag ccgcggtcga gggcggcgcc gatggtccgg ccggccttca gttcctcccg
     2221 gatgcgttca aagatgagca cgttggcgtc gaccgccatg ccgatggtca gggcgatgcc
     2281 ggcgatgccc ggcagggtga gggtctggcg gaagtaggcc atcaccgcca tcaggatgag
     2341 aatgttgact ccgagggcga agacggccgc caggccgccg gccgagtaat agacgatcat
     2401 gaaggcaccc accaggatgg cgcccagcag gctggccttg acgccggcgt tgatggaatc
     2461 gcgccccagg ctggggccga ccaccgtccg gtcgatgatc tcgatccggg ccgggagggc
     2521 gccggccttg agcaccaggg ccaggtcgtt ggcctcctgg gtggtgaacc ggcctgaaat
     2581 ctgggccttg ccgctcggga tggcctcccg gatcaccggc gccgactgga cgatgccgtc
     2641 caggacgatg gccagccgct tgttcacgtt ggcggcggtc acctcggcga acttccgggc
     2701 cccctcggag ttgaactcca ggtcgaccac cggcaacgcg ccctggctgt atccaagctg
     2761 ggcgttgctg aggtcctggc cggtcacggc cgcctggctc ttcagaagca gcggtaccga
     2821 gggcttggaa atgccgtggg catccttctc gtactggtag agaagctcgt atccttccgg
     2881 aacggtgccg gcgatggccg acttgagcag ttccgggttg tcttcaacca tcttaaattc
     2941 gaggaaggcg gtcttgccga ggatctccat cgcccggtcc gggtcgtcga ccccgggcag
     3001 ctggaccatg atccggtcct ggccgatctt ctggagcacc ggctcggcca gcccgagcga
     3061 gtccacccgg ttccggacca cctccagcgt ccggtcggtc agctccgtgg tggggatctt
     3121 cgattccacc aacggctggc gcacctggag gaccagctgc atgccgcccc gcaggtcaag
     3181 gcccaggcgg atcttctgct ggggcggcca gaagccccag atcgagaggc cgatcacggc
     3241 caggatgacg aacaaccgga tatagatacc tttcattttt cttctttaat attgtatttt
     3301 agagaaaaag aaagaacgcg gagtcggcca tgaaaaggcc gatttgatga ttatacttca
     3361 agcccggccg ccctgtcaaa ataaggcgcc agggccgggt agagggcttt gaagatccgg
     3421 taggcctccc ggtagcgtct tccggccgcc ggttcgggtc ggaaggtccg gccggtccgg
     3481 accatcccgg cgcagccctc ggctaccgag gcgaaggcgc cggcgccgca ggcggccagg
     3541 atggccgcgc ccagggcggt ggtgtcgggg ttgacggtgc gccggcagag ggcctggaag
     3601 ttgtccgcct gcatctggca ccagagggcc gagcgggcgc cgccgccgga aaggcgcacc
     3661 tcccggagtg ggagtttcag gtccttcatt atctccaggc tgtcccgcag ggcgaaggtg
     3721 actccctcca gcaccgcccg ggtgaagtgg gcccgggtgt ggcggctggt ggccccgaag
     3781 aagacggccc gggccattgg attggcgtgg ggcgtccgct ccccgttcag gtagggcagg
     3841 aagagcaggc cgtcggcgcc ggccgggatc cggccggcgg ccgcggtcat gatctcgtaa
     3901 ggatcgatcc gccggcgctg cgcctcggcc ttttcggccg ggcagaaagc gtcgcggtac
     3961 cagcgcaggg cgccgccggc cgccagcgtc acgcccatca ggtgccaggc gcccggcacc
     4021 gcgtggcaca tggtgtggat ccgtccggcc gggtcgcgcc ggatgcgttc cgagtaggca
     4081 aagaccaccc cggaggttcc cagcgtggcc gagaccacgc ccggcctgac cacgccgttg
     4141 cccaccccgc tggccgcctg gtcgcccgcc ccggccgcca ccggacagcc ggcgggcaga
     4201 ccggtccggc gggccgcctc cgggcttacc tggccggcca gcgccggcga ttccagcacc
     4261 ggcggcagcc agcggtccgg gaccccggcc gccgccatga tttcgggcga ccagttccgc
     4321 cggttgacgt cgaaaaagcc ggtgccggag ttgtcggtca cctccgaggc ggctgtgccg
     4381 gtgaggcggt agcggatgta atccttgggg agaaagaggc gggccgtccg gcggtagttg
     4441 gccggctcgt gcttctggag ccagcgcacc ttgggggcgg taaagccggt aaagagcggg
     4501 ttgccggtga tccggcggag tttttcggag ccgacggccc gttcgatttc ggccgcctca
     4561 gggccggtgc gctggtcgca ccagatcagg gccggccgca ccacccggtc ccggccgtcc
     4621 aggaataccg agccgtgcat ctggccggcc aggcccacgg ccgccagccg ccttttcagc
     4681 ggctgccgtt ccgagagctt ccgcagggcg cgcgcggtag cccgccacca ggcctccggg
     4741 tcctgttcgg cccagagggg ccgcggctcc agcagggggt atgactcatc ctgatggtca
     4801 accacccggc cgttggagtc gaccagcagg actttcaccc cgctggtgcc aacgtcaatc
     4861 cccagaaaca ggtccgcggc cattgataaa aacccgaaaa tattatatca taatgggagt
     4921 aacgggccgg tgaaagatcg cattggttcc cgcgtccgtc cgtttctttt
//
LOCUS       DAOQYN010000144         4953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_17296_length_4953_cov_3.747244, whole genome shotgun sequence.
ACCESSION   DAOQYN010000144 DAOQYN010000000
VERSION     DAOQYN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17296_length_4953_cov_3.747244"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PKN80_06410"
     CDS             complement(<1..887)
                     /locus_tag="PKN80_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNQ35682.1"
                     /translation="MGQTIAEKILSAKSGRRVTAGELCLARVDFAFAQDGTAPLAIEA
                     FRKLGGVRLAAPDRTAFFIDHSAPSPNLGVSNLHQMMRRFAAETGLRLFEAGEGVCHI
                     LVSEAGLVKCGDLLIGADSHTPTSGALNVFATGVGSTDLAATIMTGQLWLKVPETIRI
                     ELNGRLKSGVCAKDVSLFLIKTLTASGANYRALEPVGPVVKELSVDGRFTIANLAMES
                     GAKTCLFEADEKTFQWLAGHGVDPKELQPVQADPDADCRRLAFDLSELVPQLAVPHQV
                     DQVVPVSERAGLKIDQAFLG"
     gene            complement(911..2140)
                     /locus_tag="PKN80_06415"
     CDS             complement(911..2140)
                     /locus_tag="PKN80_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HNQ35683.1"
                     /translation="MAKIYLIDVTNRDGVQTSRLGLAKLQKTMINILLNQLGVFQSEM
                     GFPVTHHEINYLKANLQLAGKGALKPIRLSGWIRAIRQDVEEALKNVPIEHLNISIST
                     SDQMIDNKFMGKITRSDVIKNMVEALKACLAGGVKSVGVNAEDASRTDLDYLIEFARA
                     AKENGAHRVRYCDTLGYDTPFTIYERIQRLAGEVKLPVELHCHNDLGMAVATSVMGAK
                     AAIDAGQDAYINTTINGLGERCGNADLVAVILAVKKGAGLADAYRLDESIDLRMAWRL
                     AKYASYAFGVPIPINQIGVGANAFAHESGIHVDGALKDHRNYELYHYEELGRGEPEVI
                     ETGRMITMGEYSGIKGFRNVYGKLEIEFKYENEARKILELARYANVHTQKPLVEDELR
                     FIAQHPEEAQLLMTMTP"
     gene            complement(2187..2585)
                     /gene="rplM"
                     /locus_tag="PKN80_06420"
     CDS             complement(2187..2585)
                     /gene="rplM"
                     /locus_tag="PKN80_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNQ35684.1"
                     /translation="MGINWYLINADNQVLGRLATRVATLLMGKERLDFSPNVNCGDHV
                     VVVNAARIRVTGNKAQDKKYRRHSGYLGGLKEISYEKLLAKKPEKVIEFAVRGMLPQN
                     RLSRQVFKNLKVYAGTEHPHAAQNPVEIKL"
     gene            2743..3348
                     /locus_tag="PKN80_06425"
     CDS             2743..3348
                     /locus_tag="PKN80_06425"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNQ35685.1"
                     /translation="MSPAESRIIQIDTPGALDSLTAVLRGGGLAVFPTDTVYGLGGRI
                     SSVAALDRILALKGRPSGKPLPVLVAGLAAAERLAIFSRAARRAALEAWPGAVTLILP
                     ARRELPAAICRDGRVAVRVPGYPPLLGVLETLQEGIAGTSANLSGEPAPASLAEVPPE
                     LSDRVELVLDGGILPGRPSRIIDFTGATPMVVRDSQAGGEK"
     gene            3345..3794
                     /gene="rpiB"
                     /locus_tag="PKN80_06430"
     CDS             3345..3794
                     /gene="rpiB"
                     /locus_tag="PKN80_06430"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNQ35686.1"
                     /translation="MKIAVASDHAGLDLKEIIKEKLLKEGYDVLDCGTRTPKSVDYPV
                     FGLAGARSVAEGKADFALLFCGTGLGMTIMANKVPGIRAVNSQCLYTAGLARSHNDAN
                     VLCLGGRVVGPDLAWEIVRTFLTTPFSGGRHQRRVNLIRKQDGCLGS"
     gene            3775..>4953
                     /locus_tag="PKN80_06435"
     CDS             3775..>4953
                     /locus_tag="PKN80_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNQ35687.1"
                     /translation="MAALVREIRIDGLKRKALEELSRKYLLALGPEEMEAIQAHYRGL
                     GRNPTDIELETIAQTWSEHCIHKTFSSRIEYREDGRTELIENLLKETIFRATRELDRP
                     YCLSVFQDNAGVVELDDRYALAFKVETHNHPSALEPYGGAGTGIGGVIRDVLGVGLGA
                     RPILNTDVFCFGPLDYPHDRLPAGVLHPRRIYKGVVSGVRDYGNRMGIPTANGAILFD
                     PGFLANPLVFCGTLGLMPRDSIEKEVRPGDLILVIGGRTGRDGIHGATFSSLSLKKGI
                     SASVVQIGAPITEKRLADVLLVARDRRLYSSLTDCGAGGLSSAVGELGKTTGAEVHLE
                     KVPLKYAGLEPWEIWVSEAQERMVLAVPPEHREEIAALCAAENVEATFIGRFTGTGEL
                     R"
BASE COUNT          842 a         1522 c         1612 g          977 t
ORIGIN      
        1 cccaggaagg cctggtctat tttcaggccg gcccgttcgg aaaccgggac cacctggtca
       61 acctggtggg gaaccgccag ttgggggacc agctcggaaa gatcgaaggc caggcgccgg
      121 cagtcggcgt ccgggtcggc ctggaccggc tggagttcct tcggatcgac gccgtgcccg
      181 gcgagccact ggaaggtctt ttcatcggcc tcgaaaagac aggtcttggc ccctgattcc
      241 atggccaggt tggcgatggt gaatcttccg tccacgctca attccttcac caccgggccg
      301 accggttcca gggcccggta attggcgccg ctggcggtga gcgtcttgat caggaaaagc
      361 gagacgtcct tggcgcagac gccgctcttc agccggccgt tgagctcgat ccggatggtc
      421 tccggcacct tgagccagag ctggccggtc atgatggtgg cggccaggtc ggtcgagccg
      481 accccggtcg cgaagacgtt aagcgctccg ctggtcgggg tatggctgtc ggccccgatg
      541 agcaggtcgc cgcattttac caggccggcc tccgagacca ggatatggca gacaccctcc
      601 ccggcttcga agagcctcag tccggtttcc gccgcgaacc ggcgcatcat ctggtgcagg
      661 ttggagacgc ccaggttggg gctcggggcg ctgtggtcga tgaagaaggc ggttcggtcc
      721 ggggcggcca gcctaacccc gcccagcttc ctgaaggcct cgatggccag gggcgcggtt
      781 ccatcctggg cgaaggcgaa atcaactctg gccaggcata attccccggc cgtcacccgg
      841 cgcccgctct tggcgctcag gatcttttcg gcaattgtct gtcccatggg tttctttagc
      901 ttttcgaggt ttacggggtc atggtcatca gcaactgggc ctcttccggg tgctgggcga
      961 tgaagcgcag ctcatcctcg accaggggct tctgggtgtg gacgttggca tagcgggcca
     1021 gctccagtat cttccgcgcc tcgttttcgt acttgaattc aatctccagc ttgccgtaaa
     1081 cgttgcggaa gcccttgatg ccgctgtatt cacccatggt gatcatccgg ccggtttcga
     1141 ttacctccgg ttcgccccgt cccagctcct cgtagtggta gagctcgtaa ttccggtggt
     1201 ccttgagggc gccgtcgacg tgaatgccgg actcgtgggc gaaggcattg gcacccacgc
     1261 cgatctggtt gatcgggatc ggcaccccga aggcgtagga ggcgtacttg gccaggcgcc
     1321 aggccatgcg cagatcgatc gactcgtcga gccggtaggc gtcggccagg ccggcgccct
     1381 tcttgacggc caggatcacc gcgaccaggt cggcgttgcc gcaccgttcg cccaggccgt
     1441 tgatggtcgt gttgatgtag gcgtcctggc cggcgtcgat ggcggccttg gcccccatga
     1501 cgctggtggc gacggccatg cccaggtcgt tgtggcagtg aagctcaacc gggagcttga
     1561 cctcgccggc cagccgctgg atgcgctcat agatggtgaa cggggtgtca tagccgagcg
     1621 tatcgcagta acgcacccgg tgggcgccgt tttccttggc cgcccgggcg aactcgatca
     1681 ggtaatcgag gtcggtccgc gaagcatcct cggcattgac ccccacgctc ttgacgccgc
     1741 cggccagaca ggccttcagc gcctcgacca tgttcttgat gacatcggac cgggtgatct
     1801 tgcccatgaa cttgttgtcg atcatctggt cggaagtgga gatcgagatg ttgagatgtt
     1861 cgatcggcac gttcttgagg gcctcctcca cgtcctggcg gatggcccgg atccagccgg
     1921 acaggcggat gggtttcagc gcgcccttgc cggccagctg aaggttggcc ttcaggtaat
     1981 tgatctcgtg atgggtgacc gggaagccca tttcactctg aaagacgccc agctgattca
     2041 gcaggatgtt gatcatcgtc ttctggagct tcgccaggcc caggcgcgaa gtctgaacac
     2101 cgtcccggtt ggtgacgtcg ataaggtata tcttggccat gttttttctc ccgaaaaaag
     2161 tcggacgcgg tccgttttca aaaaaattac aacttgatct cgaccgggtt ctgggcggca
     2221 tgcggatgct cagtcccggc gtataccttc aggtttttga agacctgtct gctcagccgg
     2281 ttctggggaa gcatgcctct taccgcgaac tcgatcacct tttcgggttt cttggccagc
     2341 agtttttcgt aactgatttc cttgagaccg cccaggtaac cgctgtgccg gcggtatttc
     2401 ttgtcctggg ccttgttccc ggtgaccctg atccgggcgg cgttgacgac gacgacatgg
     2461 tcgccgcagt tgacgttggg gctgaagtca agccgttcct tgcccatcag cagggtggcc
     2521 acccgggtgg ccagccggcc cagaacctgg ttgtcagcat tgatcagata ccagttgatg
     2581 cccatttttt ctcctttggc ggtgcgggtt gaaaaccggc ggaaaaggcc gttatgactt
     2641 taacatcgaa tatttattct atccgaaata acggtaattt gtcaaaaccg gagagcaggt
     2701 attataattt gttaaaatcc acaaaaatcc ggatggaata aattgtcccc agcagagtct
     2761 cgcataatcc agatcgacac acccggcgcc cttgattcgc tgacggcggt cctgcggggc
     2821 ggcggcctgg cggtttttcc aaccgacacc gtttacggtc tgggcggccg gatcagttcg
     2881 gtggccgccc tggaccggat cctggcgctc aagggccggc cgtcggggaa acccctgccg
     2941 gtcctggtgg ccggcctggc ggcggccgag cggctggcga tcttcagccg ggccgcccgg
     3001 cgggccgccc tcgaggcctg gccgggtgcg gtcaccttga tcctgccggc ccggcgggaa
     3061 ctgcccgcgg cgatctgccg cgacggccgg gtggcggtcc gggtgccggg ctaccctccc
     3121 ctgctggggg tgctggagac actgcaggag gggatcgccg gaaccagcgc caacctttcc
     3181 ggggagccgg ccccggcgag cctggcggaa gtcccgccgg agctttccga ccgggtggag
     3241 ctggtactgg acggcgggat cctgccgggc cggccctccc ggattatcga ttttaccggg
     3301 gcgacgccga tggtcgtccg tgattctcaa gctggagggg aaaaatgaag attgcagtgg
     3361 ccagcgacca cgccggcctt gacctgaaag agattatcaa ggagaaactt ctcaaggaag
     3421 gttacgatgt cctggattgc ggtaccagga cccccaagtc ggtggattac ccggtcttcg
     3481 ggctggccgg cgcccgctcg gtggccgagg gaaaggccga tttcgccctg ctcttctgcg
     3541 ggaccggcct gggcatgacc atcatggcca acaaggtgcc gggtatccgg gcggtcaaca
     3601 gccagtgcct ttacaccgcc ggcctggccc gcagccacaa tgacgccaat gtcctctgcc
     3661 tgggcggccg ggtggtcgga ccggacctgg cctgggagat cgtgcggact tttctgacca
     3721 cgccgttcag cggcggccgc caccagcggc gggtcaacct gatccggaaa caggatggct
     3781 gccttggttc gtgaaatcag gatcgacggc ctgaaacgga aggcgctgga agagctcagc
     3841 cggaaatacc tgctggccct ggggccggag gagatggagg cgatccaggc ccactaccgc
     3901 gggctgggac gcaatccgac cgacatcgag ctcgagacca tcgcccagac ctggtcggag
     3961 cactgcatcc acaagacctt cagcagtcgg atcgaatacc gggaggacgg ccggacggaa
     4021 ttgatcgaaa acctgctcaa ggagaccatc ttccgggcca cccgggaact cgaccggccc
     4081 tattgtctct cggtcttcca ggataatgcc ggcgtggtcg agcttgacga ccggtacgcc
     4141 ctggccttca aggtggagac ccacaatcat ccttcggccc tcgaacctta cggcggagcc
     4201 ggtaccggca tcggcggggt gatccgcgac gtgctcggag tgggcctggg cgccaggccg
     4261 atcctgaata ccgatgtttt ctgcttcggc cccctcgatt atccgcacga ccggctgccg
     4321 gccggcgtgc tccatccccg gcgcatctac aagggtgtgg tcagcggggt gcgcgattac
     4381 ggaaaccgga tgggcattcc gaccgccaac ggcgccattc tcttcgatcc gggcttcctg
     4441 gccaatccgc tggtcttctg cggtacgctc ggattgatgc ccagggacag cattgaaaaa
     4501 gaggtgcggc cgggtgacct gatcctggtt atcggcggcc ggaccggccg ggacgggata
     4561 cacggcgcca ccttttcctc gctcagcctg aagaagggga tttccgcgag cgtggtccag
     4621 atcggggccc cgatcaccga aaaacgcctc gccgacgtac tgctggttgc ccgggaccgc
     4681 cgcctttaca gcagcctgac cgattgcggc gccggcggcc tttcttcggc ggtcggagag
     4741 ctcggcaaga cgaccggggc cgaggtgcac ctggagaagg tcccgctcaa gtacgccggc
     4801 ctggaaccat gggaaatctg ggtttccgag gcccaggaac ggatggtcct ggcggtgccg
     4861 cctgagcacc gagaggagat cgccgccctc tgcgccgccg agaacgtgga ggccaccttt
     4921 atcgggcgtt ttaccgggac cggcgaactt cgc
//
LOCUS       DAOQYN010000145         4922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_17442_length_4922_cov_14.459421, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000145 DAOQYN010000000
VERSION     DAOQYN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17442_length_4922_cov_14.459421"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..2011)
                     /locus_tag="PKN80_06440"
     CDS             complement(218..2011)
                     /locus_tag="PKN80_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ35688.1"
                     /translation="MKLGNGWFRRSGGFGRGLLVGFLFLLLLAGRPLAADEKPICTIA
                     VISNPYITALPAAEIKETTGRSRDFIKGRSTAGLTGAVELVNQLKPDAFVILGNLTWT
                     GSEADFKELQGYLDRIDKAVPVYLTAGPRDLPAEAPDRCRNFFKERNVTGGAVRVKEV
                     CLKFAPAAPTAAAQLDQLEKELGAAAREARGVLLFGGPWAVGTTFPEAARLQELAAKN
                     RVAAQVGFGHTHQVTLNGSLPVWTVFSTGWGSGWKLGLIRVYPKTVELSLVRDAGYPW
                     QTLTVPNPVNVPALAPADQDPHRLPFYTEDRALKPEVTFIQFSDSQLDDKSVARYKGR
                     FASDQKMSRAAVEEANRLKADLAFMTGDLTNKNTVQEWQIFNEIYSKLSMPLYTLPGN
                     HDLLRGDEYRADPDKLGDLKDATLANWAYAEQLKAEGYTGRAALYEKFTNNKGRLNYT
                     IEKNGTVFICLNNAAQEITADQLKWLKGELEKSKSARHVFVLGHYPIDPSFGNMVPEE
                     KGAAAARQLLVEYRVAGYLFGHRHGYGYRVIDGIPHIMSQDLAWGESVGYLVYHVFPD
                     RFVVGWKPLVREAFAVPVYERVVFPEPRFKK"
     gene            complement(2102..2695)
                     /locus_tag="PKN80_06445"
     CDS             complement(2102..2695)
                     /locus_tag="PKN80_06445"
                     /inference="COORDINATES: protein motif:HMM:NF022751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNQ35689.1"
                     /translation="MLLSYFQDNHFMLLISDILVTSEVLEAFFRCEPERCRGACCATG
                     EGPVPLLPEETEILAAAQVRLEGCLDEAGRALLARAGTSILYGNRLSAPLLDNGACAY
                     AVTRSGIVSCGIQAAAIPGFPKPVSCHLYPIRERFRAPYRVLFFDRWNICRPALESGR
                     RTGTRLVDFLEAALIRRFGCDFYRRLRDRLAGKESGC"
     gene            2837..4888
                     /gene="glyS"
                     /locus_tag="PKN80_06450"
     CDS             2837..4888
                     /gene="glyS"
                     /locus_tag="PKN80_06450"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNQ35690.1"
                     /translation="MEFLLEIGVEDLPADIIAGLPEQLSVRFKTRLDSHRIAFSALET
                     YATIRRMVIRASGLPEKQPDLEKEVAGPPAAVAFLPEGGLSPAGAGYCRAQGITADRL
                     LVREAGKKRTTYYIKQTEGRPVREVLSEFLPEFLAALSFPLTMRWGTGEAPAFIRPVT
                     SLLCLLNDQPIPIAFAGIKSAPFTTGHPILKPTRRRIATPAEYFEGLEETGITLDLEE
                     RCRVLADRVKKILPDDAVYQPDQLRQLAATVEYPEAGLGGTKIAGIPYPLEIVSQVIQ
                     NLKCLPLYNGRGELRPEFVIVVDGRITREIRDGFHWVLESRMEDAGFFWKEDTLLPVE
                     ARLKTLEKVVFQESVGTMSDYGRALAELTGKLAGRLGFDPAAAETLAEAGRLAKLDLT
                     TSSVREFPAVAGIMAGRLLELESVPAEVCGLVREHLYPKFPGDRLPKDRPACLLACAD
                     KTLHLCGLFLAGIEASGSADPYGLRRLAQGILELAWQSDFRLEWPELLAAAVRAWGRP
                     EGEAARIHAFFEGRLANGLENQGFRPDVAAAALGGEGRSVTSAPKRAAALTALLAETA
                     GTARLTTFSRITNIIGQAREKELEAGQVREADFTEAAERSLFEAWRKVRPEVEARLDS
                     EDYPAALAQLARLDAEINLFFDQVLVMCPDPEVRRNRLGLLAELDATLRRLGNLRRLQ
                     F"
BASE COUNT          932 a         1643 c         1534 g          813 t
ORIGIN      
        1 accgcgatgg tacagatcgg cttctcgtcg gcggccagcg gacgcccggc cagcaaaaac
       61 aggaagaaaa gtccgaccag cagcccccgg ccaaagccac ttcttcgcct gaaccagcca
      121 tttccaaatt tcatgactcc tcccaggttg gcaaaattaa aaacgcctta caatcacccg
      181 cagaaaactc gttcatttcg ttttaccccg taccggttca ctttttgaag cgcggctcgg
      241 gaaagaccac ccgctcgtag accggcacgg cgaaggcctc gcgcaccagc ggcttccagc
      301 cgaccacgaa acggtccggg aagacgtggt aaacaagata accgactgat tccccccagg
      361 caaggtcctg gctcatgatg tgcggaatac cgtcgatcac ccggtagccg taaccgtggc
      421 ggtggccgaa gagatagccg gccaccctgt attcaaccag gagctggcgc gccgcggcgg
      481 cccccttttc ctccgggacc atgttgccga aggaggggtc gatcgggtaa tggcccagca
      541 cgaagacgtg cctggccgac tttgacttct ccaattcgcc cttcagccac ttcagctggt
      601 cggcggtgat ctcctgggcg gcgttgttaa ggcagatgaa aacggtgccg ttcttctcga
      661 tcgtgtaatt gagccgcccc ttgttgttgg tgaacttctc gtagagggcg gcccggccgg
      721 tgtacccctc ggccttgagc tgctcggcat aggcccagtt ggccagggtc gcgtccttca
      781 aatcccctaa cttgtccgga tcggcccggt actcatcccc gcgcagcagg tcgtgattgc
      841 ccggcagggt gtagagcggc atgctcagtt tcgagtagat ctcgttgaag atctgccact
      901 cctggacggt attcttgttg gtcaggtcgc cggtcatgaa ggccaggtcg gccttcagcc
      961 ggttggcctc ctccaccgcc gcccggctca tcttctgatc cgaggcgaag cgtcccttgt
     1021 aacgggcgac gctcttatcg tcaagctggc tgtcggagaa ctggatgaag gtaacctcgg
     1081 gcttcagcgc ccggtcctcg gtgtagaatg gcagccggtg cggatcctgg tcggccggcg
     1141 ccagggccgg cacgttgacc gggttgggca cggtcagcgt ctgccaggga tacccggcgt
     1201 cgcgaaccag gctcaattcg accgtcttcg gatagacccg gatcaggccc agcttccacc
     1261 cggagcccca gccggtggag aagaccgtcc agaccggcag gctcccgttc agggtcacct
     1321 ggtgggtgtg tccaaaacca acctgggcgg ccacccggtt cttcgcggcc agctcctgga
     1381 ggcgggccgc ctccgggaag gtcgtgccca ccgcccaggg gccgccgaag agcaggacgc
     1441 cccgggcctc ccgggccgcc gcccccagct ccttctccag ctggtccagc tgggcggccg
     1501 ccgtcggcgc cgccggggcg aacttcagac agacctcctt gacccggacc gcaccgccgg
     1561 tgacattccg ctccttgaaa aagttccggc atcgatccgg ggcctcggcc ggcaggtcgc
     1621 gcggaccggc ggtcaggtaa accgggaccg ccttatcaat acggtcgagg tatccctgga
     1681 gttccttgaa gtcggcctcc gagccggtcc aggtcagatt gcccaggatg acgaaggcat
     1741 ccggcttgag ctggttgacc agctccaccg cgccggtcaa gccggccgtg gagcgcccct
     1801 tgataaagtc acggctgcgg ccggtggtct ccttgatctc ggccgccggc agggcagtta
     1861 tgtaggggtt ggaaataacc gcgatggtac agatcggctt ctcgtcggcg gccagcggac
     1921 gcccggccag cagcagcagg aagagaaatc cgaccagcag accccggcca aaaccgccgc
     1981 ttcgcctgaa ccagccattt ccaagtttca tgtcccctct ccggttggca aaataaaaaa
     2041 cgctcttttc tctgagagaa accttgtcta tactctttga taccaggacc agtcaaatcg
     2101 cttaacaacc ggactctttt ccagccagac gatcccggag acgccggtaa aaatcgcaac
     2161 cgaagcggcg aatcagggcc gcttccagga aatcgaccag gcgcgtgccg gtgcgccggc
     2221 ccgactcgag ggccggccgg cagatgttcc agcggtcaaa gaacagcacc cggtagggag
     2281 cgcgaaaacg ctcccggatg ggatacaggt ggcagctgac cggtttggga aagcccggga
     2341 tggccgccgc ctggataccg caggaaacaa tcccgctccg ggtgaccgcg taggcgcagg
     2401 cgccgttatc cagcagcggc gccgagaggc ggttcccgta aaggatcgag gtgccggccc
     2461 gggccagaag cgcccggccg gcctcatcca ggcagccctc caaccggacc tgggccgccg
     2521 ccaggatctc cgtctcttcc ggcagcagcg gcaccggccc ctcgccggta gcacaacagg
     2581 cgccacggca tcgttcgggc tcgcatcgaa agaaggcctc caggacctcg gaggtgacca
     2641 ggatgtcgga gatcagcagc atgaaatggt tatcttgaaa gtaggatagt aacatcccgg
     2701 ataaaagggc gtcaaccagc gccggcgggc ctgcctttgc ctgcccgccg gccttggagt
     2761 ataatttaat gattataccg gcaacgatca accggggccg gacaaaaaaa tcaagaagcg
     2821 aaaaaaataa ctgaatatgg aattcttgct cgaaatcggc gtcgaagacc tgccggccga
     2881 catcatcgcc ggcctgccgg aacaattgtc cgtccggttc aagacccggc tcgactccca
     2941 ccggattgcc ttctccgcgc tggaaaccta cgccaccatc cgccggatgg tgatccgggc
     3001 ttcgggcctg ccggagaagc agcccgacct ggaaaaagaa gtggccggac cgccggcggc
     3061 ggtggccttt cttccggagg gcggactcag cccggccggg gccggctact gccgggccca
     3121 gggcatcacc gccgaccgcc tgctggtaag ggaggcgggc aagaaacgga cgacctacta
     3181 tatcaagcag accgaaggcc ggccggtccg ggaggtgctt tccgagttcc tgcccgaatt
     3241 tctggccgcg ctcagcttcc cgctgaccat gcgctgggga acgggcgagg cgcccgcctt
     3301 catccggccg gtcacctccc tgctctgcct gctgaacgac cagccgatcc cgatcgcctt
     3361 cgccggtatc aagtccgctc ccttcaccac cggccacccg attctgaaac cgacccggcg
     3421 gcgcatcgcc accccggccg aatactttga aggactcgaa gaaacaggca ttacgctcga
     3481 cctcgaggaa cgctgccggg tactggccga tcgggtcaaa aaaatcctgc ccgatgacgc
     3541 cgtttaccag ccggaccagc tgcgccaact ggccgccacc gtcgagtacc cggaggccgg
     3601 gctcggcggc acgaagatcg ccgggatacc ctacccgctg gagatcgtct cccaggtcat
     3661 ccagaacctc aagtgcctgc ccctttacaa cggacggggc gaactccgcc cggaattcgt
     3721 gatcgtcgtc gacggccgca tcaccaggga aatccgggac ggtttccact gggtcctgga
     3781 gagccggatg gaggatgccg gattcttctg gaaggaggac acgctactcc cggtcgaagc
     3841 cagattgaag acgctggaga aggtcgtctt ccaggagagc gtcggcacca tgtccgatta
     3901 tggccgggca ctggccgaac tgaccggaaa actggccggc cggctcggct tcgacccggc
     3961 ggcggccgaa accctggccg aagcgggccg gctggccaag ctggacctca ccaccagttc
     4021 ggtccgggag ttcccagcgg tggccggcat catggccggc cgcctgctgg aattggaatc
     4081 ggtcccggcc gaggtctgcg gcctggtaag agaacacctt tatccgaagt tccccggcga
     4141 tcggctgccg aaggaccggc ccgcctgcct gctggcctgc gccgacaaga cgctccacct
     4201 ctgcggcctc ttcctggccg gcatcgaggc ctccggatcg gccgatccct atggattgcg
     4261 ccggctggcc cagggaatcc ttgaactggc ctggcagtcc gatttccgcc tcgagtggcc
     4321 ggaactgctg gccgcggccg tgcgggcctg gggacggccg gaaggcgagg cggcccggat
     4381 ccacgccttc ttcgagggac ggctggccaa cgggctggag aaccagggct tccggcccga
     4441 cgtggccgcc gcggccctgg gcggcgaagg ccggagcgtg acctcggcgc cgaaacgggc
     4501 ggccgcgctg accgccctgc tggccgaaac cgccggcacc gcccgcctga ccaccttcag
     4561 ccggatcacc aacatcatcg gccaggcgcg ggagaaggag ctggaagccg gccaggtccg
     4621 tgaggcggat ttcaccgagg cggccgagcg ttcgctcttc gaggcctggc gaaaagtccg
     4681 cccggaagtc gaagcacgcc tggattccga ggattacccg gccgccctgg cgcaactcgc
     4741 ccggctggac gccgaaatca atcttttctt cgaccaggtg ctggtgatgt gcccggaccc
     4801 ggaagtacgc cgcaaccggc tgggcctcct ggccgaactg gacgcgaccc tccggcggct
     4861 cggcaacctc aggcgcctcc aattttaaaa cctgtcgggt gaacatggac ttgaaaccga
     4921 tc
//
LOCUS       DAOQYN010000146         4904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_17530_length_4904_cov_4.697876, whole genome shotgun sequence.
ACCESSION   DAOQYN010000146 DAOQYN010000000
VERSION     DAOQYN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17530_length_4904_cov_4.697876"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="PKN80_06455"
     CDS             <1..1704
                     /locus_tag="PKN80_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ35691.1"
                     /translation="LYPATLLYIWKAALPYKVPYDDELRHRVAYNSQGDHADYRGSNQ
                     TEFNHLFHNYNDELHLRNMVKIAAGMDVAPPVALLKPLEVKGGTVVYDTLKTALLIRQ
                     GEDEEIRIRLSAADSYDLQGWPLTFRWKVLYGHRDVQIRREGETAEYTLVIPFDKQLP
                     RGRTSILLIANNGRYDSNPAVVNIYRTAGKDNLRPTLEGLRDLTILPGERAIFRLSGR
                     DPEGFPVVFSQWDGEVGRIEGDTFTWQCPPFHPAGVEPVTVIVSDGSAGNGYNSGRAF
                     ITVSPTAACLRPDPPQGPAPLRVSFSSEGSRDLKNGPLRYEWNFDDGSVSGEPNPVHT
                     FTPPGFYEVSLTVTGPSGRHTARSVVRVEPQWPLAIENGWGKDGIDRAVWQLTDPDTA
                     IVHVSGRDGGFVKIYDPKLEKPGPHGLTSLKKLAPPLYLEASFFSVLDGIRPGTGFSI
                     LGTQLGYIEAPGESGRNISIGRPLAAGGWASRFIASKVRFPWQKSNLRLFLDADPAHP
                     GRFRYTGFLESDTGENFFRLDNQELPSEGPISIVSGSARSRFELYSIRIWAGKTPSPA
                     R"
     gene            1837..3261
                     /locus_tag="PKN80_06460"
     CDS             1837..3261
                     /locus_tag="PKN80_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35692.1"
                     /translation="MDRKIRCLHIRGVSGAVRLFFSCLLLAGLAARFVPAARAAEWVT
                     STLDDAGDYSSLALDSNGKAHASYFDGGDLKYATNASGAWVVSTLDSPGTGGGLTYAS
                     SIAVDGNGKVHIAYDGTQQLKYATNASGAWVITTLDNPGYDVGDDLSLALDSNNKVHI
                     SYLDRNGGSLKYATDLTGSWTLSNLGSGGYVTSITVDGNDKVHIVHFRAGNQLTYTTN
                     ASGSWVSTVIGVNGGYFASVKTDSANKVHVSYYASGAVNYMTNASGAWVNTVIDNAVG
                     IAGNSSLVLDSLGGVHISYCNYNDNDLQYATNASGAWLNSIADQEGNVGYFSSLAIDG
                     QDLLYVTYTDRTNGKVKFAYTLAGEEEEESDNLCFIATAAFGSPLAGQVALLRRFRDH
                     CLLTNRPGAAFVSWYYRKGPLAARYIAPRPLARAAVRLVLYPLIAFSFLALNGWLLPG
                     LILLAAVGAWLFWVQERRRTPAVL"
     gene            complement(3462..>4904)
                     /locus_tag="PKN80_06465"
     CDS             complement(3462..>4904)
                     /locus_tag="PKN80_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNQ35693.1"
                     /translation="PNSQWVTFKKSLSNDYPIFIININTRQVYQLTEGYYDGRMWWSP
                     DGLKILFKEKEQSSNSRDGGLYNYDLLVINLSESFAWNAVQILSVLGGGGGCFIATAA
                     YGSYQEPHVKVLREFRDRYLLTNAAGRAFVRFYYRHSPAIANLIAGNEALRAATRTAL
                     LPVYGLAWFLLKGWLLPAAMLAAGLLLGWLAMRRRPLAAAGLTLALLLAASPVLALDA
                     NHFSPALGEEVFTAVPSADTLEVGTKDFGLFVSYAEGPLKFKAGGGGDEQSLSSHQAF
                     AVLGFAAGLTDRFQLGARLPFLFSQDSALKGAAEPDETGLGDLRIEGKYRLAGGDAAG
                     VAIVPYLSLDTGSDDAFFSKGANALGVLLVVDRNWNRQTWFTAHVGLQTQGDEDFQDV
                     EIENSLLFGTGLGWKLASSGSALSLEIFGRADGSDLFDREEATPIEALFSYTKEIRER
                     ARLKLGLGKGLTKGYGAPDYRLFIGMERAF"
BASE COUNT          876 a         1609 c         1509 g          910 t
ORIGIN      
        1 ctttacccgg ccacgctcct ctacatctgg aaggccgccc tgccgtacaa ggttccctat
       61 gacgacgaat tgaggcaccg ggtggcctac aattcccagg gggatcacgc cgattaccgg
      121 ggaagcaacc agaccgagtt caaccacctc tttcataatt acaacgacga acttcacctc
      181 cggaacatgg tgaaaatagc cgccggcatg gatgtcgcgc cgccggtcgc gctcctgaag
      241 ccgctggagg tcaagggcgg caccgtggtt tacgacacgc tgaagacggc cctgctgatc
      301 cggcaggggg aggacgaaga gatcaggatc cgcctttcgg ccgccgattc ctacgacctg
      361 cagggctggc cgctcacctt ccgctggaag gtcctttacg gccaccgtga cgtgcagatc
      421 cggcgcgagg gcgaaacggc cgagtacacc cttgtcatcc cgttcgacaa gcagcttccc
      481 agggggcgca cttccattct gctgatcgcc aacaacggcc gttacgacag caacccggcg
      541 gtcgtgaaca tctaccggac cgccggcaag gataacctgc gcccgaccct ggagggtctg
      601 cgcgacctga ccattcttcc gggcgagcgc gccattttcc gcctttccgg ccgggatccg
      661 gagggcttcc cggtggtctt ctcccagtgg gacggggagg tcggccgcat cgagggcgac
      721 accttcacct ggcagtgtcc gcctttccac ccggccggag tcgagccggt gacggtcatc
      781 gtctccgacg gaagcgccgg caacggatac aacagcggac gggccttcat aacggtcagt
      841 ccgacggccg cctgcctccg gcccgatcct ccgcagggtc cggcgcccct gcgggtgtcc
      901 ttctcttcgg agggatcacg cgacctgaag aatgggccgc tccggtatga atggaacttc
      961 gacgacggaa gcgtttccgg ggagccgaac ccggtccaca ccttcacgcc cccgggtttt
     1021 tacgaggtgt cccttaccgt caccgggcca tccggccgcc acaccgcccg gagcgtggtc
     1081 cgggtcgagc cgcaatggcc gctggccatt gaaaacggct ggggcaaaga cgggattgac
     1141 cgggccgtct ggcagctgac cgatccggac accgccatcg tgcacgtctc cggccgggat
     1201 ggcggcttcg tcaagatcta cgaccccaag ctggaaaagc ccggccccca cggtttgacc
     1261 agcctgaaga aattagcgcc gcctctctac ctggaggcct ccttcttctc cgttctcgac
     1321 ggcatccgtc cggggaccgg tttttcgatc ctggggaccc agctcggtta tatcgaggcg
     1381 cccggggagt ccggccgcaa catctcgatc ggccgtccgc tcgcggccgg aggctgggcg
     1441 agccggttca ttgcctccaa ggtgcgtttc ccctggcaga aatcgaacct gcgtctcttc
     1501 ctggatgcgg atccggccca tccgggccgc ttccgttaca ccggcttcct ggaatcggac
     1561 accggcgaaa acttcttccg gcttgacaac caggagttgc cgtcggaggg gccgatttcc
     1621 attgtcagcg gcagcgcccg gtcgcgtttc gagctttatt cgatccggat ctgggcggga
     1681 aaaacgccgt ccccggcgcg atagccacgg cgtcgtctgc tccggtattg actggcgaaa
     1741 aaggcggcgg tataaactag agatggccgc gcgggaaata ttccatcgag atccggtcgt
     1801 taggcccggc tgaaaatcgc aaaagggagg agaggcatgg atagaaagat ccgttgtttg
     1861 catatccggg gagtcagtgg cgccgtccgg ctcttttttt cctgcctgct gctggccggt
     1921 ctggccgccc ggttcgttcc ggccgcccgg gccgccgaat gggttacctc gaccctggac
     1981 gacgccgggg attacagttc gctggcgctc gattcgaatg gcaaggccca cgccagttac
     2041 tttgacggcg gcgacctcaa gtatgccacc aacgccagcg gggcctgggt cgtctccacc
     2101 ctggacagcc ccgggaccgg cggcgggctt acctacgcat cctccatcgc ggttgacggc
     2161 aacggcaagg ttcacatcgc ctatgacggc acccagcagc tcaagtacgc caccaacgcc
     2221 agcggggcct gggtcatcac caccctcgac aacccgggtt acgatgtcgg ggatgatctt
     2281 tccctcgccc tggattcgaa caacaaggtc cacataagtt acctggaccg gaacggtgga
     2341 tccttgaagt acgcgaccga cctgaccggc agctggacgc tttccaacct gggctcgggc
     2401 gggtacgtga cttccatcac ggttgacggc aacgacaagg tccacatcgt ccatttccga
     2461 gccggtaacc agctgactta caccaccaac gcttccggtt cctgggtcag cacggtcatc
     2521 ggcgtcaacg gcggctattt tgcctccgtg aaaacggaca gcgccaacaa ggtccatgtc
     2581 agttattacg cctccggggc ggtgaattac atgaccaacg cctcgggcgc ctgggtcaat
     2641 accgtcattg ataacgccgt cggcatcgcc ggtaactcct cgttggttct cgattccctt
     2701 ggcggcgtcc atatcagtta ctgcaactac aacgacaatg acctccagta cgccaccaac
     2761 gccagcgggg cctggctgaa ttccatcgcc gatcaggagg gtaatgtcgg ctatttttct
     2821 tccctggcga ttgacggcca ggacctgctt tacgtcactt atacggatcg gaccaacggc
     2881 aaggtaaaat tcgcctacac gctggccgga gaggaggaag aggaatccga caacctctgt
     2941 tttatcgcca cggccgcctt tggttccccg ctggccggcc aggttgctct tctgcgccgg
     3001 tttcgcgacc attgtctgct taccaaccgt ccgggtgcgg ccttcgtctc ctggtattac
     3061 cgcaagggtc cgctggccgc ccggtatatt gcgccccggc cgctggcccg ggccgcggtc
     3121 cggctggtcc tctacccgtt gatcgccttc tctttcctgg cgcttaacgg ttggttgctg
     3181 cccggattga tacttttggc cgccgtcggc gcctggctgt tctgggtgca ggagagaaga
     3241 aggactccgg ccgttttgta aaagagaggc ggcgaccccg ggcgatgttt tcagccccga
     3301 gacccgatcc gggtctcggg gctgattttt aggcgcggct ggccgaaatc gatcgaggcg
     3361 gcttcttatc cggccggtat ccgcccggga agataaaaaa agcagggcgg ggaatttttc
     3421 ttccccgccc tgccgaagaa ctttccttac ctggatcggc cttagaaggc tctttccatt
     3481 ccgatgaaga gccggtaatc gggggccccg tatcccttgg tgagaccctt gcccaggccc
     3541 agcttgaggc gggccctctc gcggatctcc ttggtgtagg agaagagcgc ctcgatcggg
     3601 gtcgcctcct cccggtcgaa gagatccgac ccgtcggccc ggccgaagat ctccaggctc
     3661 agcgcgcttc cggaggaggc gagcttccag cccaggccgg ttccgaagag gagagagttc
     3721 tcgatttcga catcctggaa atcctcgtcg ccctgggtct ggagaccgac gtgggcggta
     3781 aaccaggtct gccggttcca gttgcgatcc accaccagca gcacgcccag ggcgttggcc
     3841 cccttgctga agaaggcgtc gtcgctgccg gtatccaggc tcaggtaggg cacgatggcc
     3901 accccggccg cgtcaccgcc ggccaggcgg tacttgccct cgatcctcag gtcacccagg
     3961 ccggtttcgt ccggctcggc ggcccccttg agggccgagt cctggctgaa gaggaagggc
     4021 agcctggccc cgagctggaa ccggtcggtc aatccggccg cgaatccgag cacggcaaag
     4081 gcctggtggc tggagaggga ctgctcgtca ccgccgccgc cggccttgaa cttgagcggc
     4141 ccctcggcgt agctgacaaa gaggccgaaa tccttcgttc ctacctcaag ggtgtccgcg
     4201 ctcggcaccg ccgtgaagac ctcctcgccc agcgccggcg agaagtggtt ggcgtccagg
     4261 gccagcaccg gcgaggcggc cagcagcagg gccagggtca gcccggccgc ggccagcggc
     4321 cgccgccgca tcgccagcca gcccagcagg agtccagccg ccagcattgc ggccggcagc
     4381 agccagccct tgagcaggaa ccaggccagc ccgtaaaccg gcagcagggc cgtccgggtc
     4441 gccgcccgca gcgcctcatt gccggcgatc aggttggcga tggccggcga gtggcggtag
     4501 tagaagcgga cgaaggcccg acccgccgcg ttggtcagca ggtagcggtc ccggaattcc
     4561 cgcaggacct tgacgtgcgg ctcctggtag gaaccgtaag cggccgtcgc gatgaagcac
     4621 ccgccgccgc cccccagcac cgagagaatc tgcaccgcgt tccacgcgaa cgactcggac
     4681 aggttgatga ccagcaggtc gtaattgtag aggccgccgt cccgggaatt ggaactctgc
     4741 tccttctcct tgaagagtat cttcaaaccg tccggactcc accacatccg gccgtcgtaa
     4801 tacccttcgg tcagttgata aacttgccgg gtgttgatgt tgatgatgaa gatcgggtag
     4861 tcattagaca gcgatttctt gaaggttacc cactgggagt tggg
//
LOCUS       DAOQYN010000147         4869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_17684_length_4869_cov_3.861238, whole genome shotgun sequence.
ACCESSION   DAOQYN010000147 DAOQYN010000000
VERSION     DAOQYN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17684_length_4869_cov_3.861238"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1986)
                     /locus_tag="PKN80_06470"
     CDS             complement(<1..1986)
                     /locus_tag="PKN80_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNQ35694.1"
                     /translation="MSDWKNPEVLDRPGPFWSWNDKLSEAELLRQIREMAAKGWGSYF
                     MHSRVGLVTGYLSEEWLVLVAACAREAERTGTRAWLYDEDKWPSGFAGGLVPLADPAY
                     RGRALLLVRAGKAGVADRVLASFQWNGADYQIVNRVMELGDPWFNGACYVDLMNPAAV
                     RAFLEATHERYREACGEHFGRAIPGVFTDEPCYSFFGQYREPAVPWSESLPDFFRKRH
                     GYDLLERLPELFFPTGDYRQTRLDFYTAASALFRESFTRQYHNWCRENKLIFTGHFMA
                     EDTLVSQTQWSGDVMAHYEFMDWPGIDKLGRHIDQNVTVKQVSSAAEQLGKARAFCEV
                     YGCMGGQASFFHRKWIGDWQAVLGIGFVNHHLSLYSMRGERKRDYPANLFYQQPWWND
                     EKEFADYQGRLCRAVTEGKRLVDVLVIQPLTSVWCEYSPVDRGNGWASASVYDRPFAD
                     LTLRLLQEKIDFHYGNESLLAEHGRVEGEKLRLKEHAYSTVVVPPALNLNGATLDLLT
                     RFAAAGGFVLFMGRRPELVDGRPGPVEIPSAQLAGSLDETVELVAARCPDRVSVLDRR
                     TGRSAPAVYVQTRRVGDGLRHLLVNVDEKRAILVSARLPGLAAGAAVLDLSDGRSYRV
                     PSGSDSLEFELAPAGSLLVLAGPEADSAEPVWPDLLGS"
     gene            complement(2017..4362)
                     /locus_tag="PKN80_06475"
     CDS             complement(2017..4362)
                     /locus_tag="PKN80_06475"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNQ35695.1"
                     /translation="MDLYGRLNRELDEKARAGLLKAERVLGSPQGSEIMVEGIREPVL
                     NFCSNNYLGLANHPAVLAAVRRALEVYGFGLASVRFICGTQTIHKQLEERLADFLGLE
                     DCLLYTSCFDANGGLFAGTLAETDTIVSARLNHASIIDGVRLTRCRRFTYREDDPADL
                     ERTLAEVGGEGVKLVMTDGVFSMEGTLAPLDGLAPVCRKHGALLAVDDSHATGFVGRT
                     GRGTPEHFGLPVDIVTGTLGKALGGATGGFTAGRRVLIDWLRNTSRPYLFSNSLPPAL
                     AAGAIAALDVAASPEGDQLRVRLAENTARFRSALREAGFSVPEGGHPIVPVMVGDASL
                     AGRMAGRLLEQGIYVISFSYPVVPEGKARIRVQVSALHTPEQLDRAAAAFARAGREFG
                     LVRKAPVPVRGSVRPELPEKMQAWVYYNQAGADGGHLRLEEVAVPRPHPGELLLKVLG
                     VTVCGTDEDLFHGKFQEVHDCVIPGHEIFGSVAALGSNVRGFEIGQKLCVESHYQIPG
                     YLEEGIIGLWGPKIARGGYLRPLNGGYAEYVKIPTYCAHPIPEVLDHPGFLPSILEGV
                     GNDFLIAKYLLDQGLLGTVGVVGCGPHGLFTQMFLRHFGVARLAAFEVNAARLKLAAE
                     FGVDLGLDARVADLDRRVADFTGGAGFDVMVDIAGGQRGVLETCLKYLRDRGTLVLFG
                     LYGDPAIQLAGQPINDIIFNQRELQLEHAGKRIRVRGITGREGIWDSLIDTVALSRPL
                     QDKIMKLVTVMGTLEKLGGDTLALDRRRIMKRGYRPFGAGF"
     gene            complement(4399..>4869)
                     /locus_tag="PKN80_06480"
     CDS             complement(4399..>4869)
                     /locus_tag="PKN80_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNQ35696.1"
                     /translation="IIEDRPETALVVAPIGGGGLAAGLLVALKSAAPRLRLIGVQAAG
                     APSARLSLRAGRIVCLESIDTYANGIAVKRPGEICFEIISTLIDDIVLVTDEQMRTAQ
                     KRLYRSAGIVAEGAGAASLAAVLAGLAGPERPVVCLVSGGNASAGEVAALTAAD"
BASE COUNT          832 a         1604 c         1689 g          744 t
ORIGIN      
        1 gctgcccagc agatcgggcc agaccggttc ggccgaatcg gcttcggggc cggcaagcac
       61 cagcaggctc ccggccggcg ccagctcgaa ttccagggag tccgaaccgg aaggaacccg
      121 gtaactgcgt ccatcggaca ggtctagcac cgccgcgccg gcggccaggc cgggcagccg
      181 ggcggaaacc aggatcgctc tcttttcatc gacgttgacc agcaggtggc ggagtccgtc
      241 acccacccgg cgtgtctgga catagaccgc cggagcgctg cggccggtcc ggcggtccag
      301 caccgagacc cggtccgggc agcgggcggc caccagctcg accgtttcat ccagagaacc
      361 ggccagctgg gcggaaggta tctccaccgg cccgggccgg ccatccacca gttcgggccg
      421 ccggcccatg aagagcacga aaccgccggc ggcggcgaag cgggtcagca ggtccagagt
      481 ggccccgttg aggttcaagg ccggcggcac caccaccgtg gaataggcat gctctttcag
      541 gcgcagcttc tccccctcca cccggccgtg ttcggccagc agggattcgt tgccgtaatg
      601 aaaatcgatt ttttcctgga gcaggcggag cgtgagatcg gcgaaggggc ggtcgtaaac
      661 ggaggcggac gcccagccgt tgccccggtc gaccggagaa tattcgcacc agacgctggt
      721 caggggctgg atgaccagca cgtcgaccag ccgttttccc tcggtgaccg cccggcagag
      781 gcgcccctgg taatcggcga attccttttc gtcgttccac cagggctgct ggtagaagag
      841 gttggccggg taatcgcgct tccgttcccc gcgcatcgag tagagcgaca ggtggtggtt
      901 gacgaaaccg atgcccagga ccgcctgcca gtcgccgatc cacttgcggt ggaagaagct
      961 ggcctggccg cccatgcagc cgtaaacctc gcagaaggcc cgcgccttgc ccagctgttc
     1021 ggcggccgag ctgacctgct tgacggtgac gttctggtcg atgtggcggc ccagcttgtc
     1081 gatgcccggc cagtccatga attcgtagtg ggccatcacg tcgcccgacc actgggtctg
     1141 ggagaccagg gtgtcctcgg ccatgaagtg gccggtaaag atgagtttgt tctcccggca
     1201 ccagttatga tactggcggg tgaagctctc ccggaagagg gccgaagcgg ccgtgtagaa
     1261 atcgaggcgg gtctgccggt aatcgccggt cggaaagaag agctccggga ggcgttccag
     1321 caggtcgtaa ccgtgacgct tcctgaagaa gtccggcagg ctctccgacc aggggacggc
     1381 cggttcccgg tactggccga agaaggaata gcatggctcg tcggtgaaga ccccggggat
     1441 ggcgcggccg aagtgctctc cgcaggcttc ccggtaacgc tcatgggtgg cctcgaggaa
     1501 ggcgcggacc gccgccgggt tcatcaggtc cacgtagcag gcgccgttga accagggatc
     1561 gcccagttcc atgacccggt taacgatctg gtaatcggcg ccgttccact ggaaggaggc
     1621 cagcacccgg tcggcgacgc cggcctttcc ggcccgcacc agcagcaggg cccggccgcg
     1681 gtaggccgga tcggccaggg gaaccaggcc gccggcgaag ccggagggcc acttgtcctc
     1741 gtcgtagagc caggcacggg tgccggtgcg ctccgcctcg cgcgcgcagg cggcgaccag
     1801 gaccagccac tcctccgaaa ggtagccggt caccagaccg acccgggagt gcatgaagta
     1861 cgacccccag cccttggccg ccatctccct gatctggcgg aggagctccg cctcggagag
     1921 cttgtcgttc cagctccaga acgggccggg ccggtccagg acctccgggt tcttccagtc
     1981 ggacatttta cactcctttt tgattaacga agcgcttcag aaaccggcgc cgaagggccg
     2041 atagccgcgt ttcatgatcc ggcggcggtc cagtgccagg gtgtcgccgc cgagtttctc
     2101 gagcgtcccc atgaccgtca ccagcttcat gatcttatcc tgcagcggcc gggagagggc
     2161 gaccgtatcg atcagtgaat cccagatccc ctcgcggccg gtgatgcccc gaacccggat
     2221 ccgcttgccc gcgtgctcga gttggagttc acgctggttg aagatgatgt cgttgatcgg
     2281 ctggccggcc agctgaatgg cgggatcgcc gtagaggccg aagaggacca gggtcccgcg
     2341 gtcacgcaag tacttcaggc aggtttccag gacgccgcgc tggccgccgg cgatgtcgac
     2401 catcacgtcg aaaccggcgc cgccggtgaa gtcggccacc cgccggtcga gatcggcgac
     2461 gcgggcgtcc aggccgaggt cgacgccgaa ttcggccgcc agcttcagcc gggcggcgtt
     2521 gacctcgaag gcggccaggc gcgccacgcc gaagtggcgg aggaacatct gggtgaagag
     2581 gccgtgcgga ccgcagccga ccacgccgac ggtccccagc aggccctggt cgagcaggta
     2641 cttggcgatc aggaaatcgt tgccgacgcc ctccaggatc gagggcagaa agcctgggtg
     2701 gtcgaggacc tccggaatcg ggtgggcgca gtaggtcggg atcttgacgt actcggcgta
     2761 accgccgttg agcggccgca ggtagccgcc ccgggcgatc ttcggacccc agaggccgat
     2821 gatgccttcc tcgaggtagc cgggaatctg gtagtgggat tcgacgcaga gcttctggcc
     2881 gatctcgaaa ccgcgcacgt tggagccgag ggcggccacc gagccgaaga tttcgtggcc
     2941 ggggatgacg cagtcgtgaa cctcctggaa cttgccgtgg aagaggtcct cgtcggtacc
     3001 gcagacggtc accccaagca ccttcaaaag caattccccc gggtggggcc ggggaaccgc
     3061 cacctcttcc aggcgcaggt ggccaccgtc ggccccggcc tggttgtagt aaacccaggc
     3121 ctgcatcttc tccggcagct cgggccggac cgaaccacgg acgggcaccg gggccttccg
     3181 aaccaggccg aactcccggc cggcccgggc gaaggcggcc gcggcccggt ccagctgctc
     3241 cggggtgtgc agggccgaaa cctggacccg gatgcgggcc ttgccctcgg gcaccaccgg
     3301 gtagctgaag ctgatgacat agatgccctg ctccagcagg cggccggcca tccggccggc
     3361 cagggacgcg tcgccgacca tgaccggcac gatcgggtga ccaccttccg gaaccgagaa
     3421 gccggcctcg cgcagggcgg accggaaacg ggcggtgttt tcggccaaac ggacccgcag
     3481 ctggtcaccc tccggcgaag cggccacgtc gagggcggcg atggcgccgg cggccaaggc
     3541 cggcggcagg gagttggaaa agagataggg acgcgaggtg ttgcgcagcc agtcgatcag
     3601 gacgcggcgg ccggcggtga agccgccggt ggccccgccc agcgccttgc ccagggtgcc
     3661 ggtgacgatg tcgaccggga ggccgaagtg ttccggggtg cctcggccgg tgcggcccac
     3721 gaagccggtg gcgtgcgaat cgtcgaccgc cagcagggcg ccgtgcttcc ggcagacggg
     3781 cgccagccca tccagggggg ccagcgttcc ctccatgctg aagacgccgt cggtcatcac
     3841 cagcttaaca ccctcgccgc cgacctcggc cagcgtccgt tccaggtcgg ccgggtcgtc
     3901 ttcgcggtag gtgaagcggc ggcagcgggt caggcggacg ccgtcgatga tgctggcgtg
     3961 gttgagccgg gcgctgacga tggtgtccgt ctcggcgagg gtgccggcga agaggccgcc
     4021 gttggcgtca aaacaggagg tgtagagaag gcagtcctcc aggcccagga agtcggccag
     4081 gcgctcctcc agctgcttgt ggatggtctg ggtgccgcag atgaaccgaa ccgaggccag
     4141 cccgaatccg taaacctcca gggcccggcg caccgcggcc agcaccgccg ggtggttggc
     4201 caggcccagg tagttgttgg agcagaagtt gagaaccggc tcccgaatgc cttcgaccat
     4261 gatctcggag ccctggggag aaccgagcac tcgttccgcc ttcaataatc cggccctggc
     4321 cttttcgtcc agttcccggt ttaaccgtcc gtagaggtcc attttcgttc gactccttga
     4381 ttattgatga aaagtcattc aatccgcggc ggtcagcgcg gccacttccc cggccgaggc
     4441 gttcccgccg ctgaccaggc agacaaccgg tcgctccggt ccggccaggc cggccagcac
     4501 cgcggccagg gaggccgcgc cggccccttc ggccacgatc ccggccgacc ggtaaaggcg
     4561 tttctgggcg gtccgcatct gttcgtcggt aaccagcacg atatcgtcta tcagggtgga
     4621 gatgatctca aagcagattt cgccggggcg cttgaccgcg atgccgttgg cgtaggtatc
     4681 gatgctctcc aggcagacga tccgaccggc ccggagcgaa aggcgggccg aaggggctcc
     4741 cgccgcctgg acgccgatca gccggagccg gggtgcggcc gatttcaggg ccaccagcag
     4801 cccggcggcc aggccgccgc cgccgatggg cgcaaccacc agggcggtct ccggccgatc
     4861 ctcgatgat
//
LOCUS       DAOQYN010000148         4833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_17843_length_4833_cov_4.808497, whole genome shotgun sequence.
ACCESSION   DAOQYN010000148 DAOQYN010000000
VERSION     DAOQYN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17843_length_4833_cov_4.808497"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PKN80_06485"
     CDS             complement(<1..568)
                     /locus_tag="PKN80_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNQ35697.1"
                     /translation="MSKGISRTEFQERWRRLQAGLAKLDLDAALVHSNEADFANVRYL
                     SDYWPIFESAGVFIPRQGEPVLLIGPESETFAASHSRIPGFRMLLEYRESAEPEYPTI
                     PVSSFREVFREGMAGRKLKRLGIIGHSIFPAFIQDKLRKEFPGLELVKADRQLGNESG
                     IAVWAKAAADGVAILINGFQVLFRIIKID"
     gene            complement(571..1596)
                     /locus_tag="PKN80_06490"
     CDS             complement(571..1596)
                     /locus_tag="PKN80_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ35698.1"
                     /translation="MASRRVRLKDIAARAGVSAMTVSRVLNQHAHVSEVVRARVLQLV
                     KEYDYQPNFIARSLVSRRSRTVGFLVPAINLSYFFEIIRGIETVFGRNGYHLILGHTG
                     EDAARERAELGVLRSRQVDGLIIAPALNVNDIEPYLALQKLELPAVFVDRCFPGLECS
                     YVYSNDQAGGRLVGELLLRLGHRRIAFLAAALRDPEYCRMKSCRDFLEAAGVKTDRRL
                     VRLAGVTRADGYREAGHLLGLAAPPTAVFAHNDEVAIGVYQRAREMKIRIPGDLTVIG
                     YGNLHWSGYVDPPLTTVDQKPFGIGSEAARVLLERIEKRGTRPTRILVEPELITRGSH
                     AACQPRR"
     gene            1756..2505
                     /locus_tag="PKN80_06495"
     CDS             1756..2505
                     /locus_tag="PKN80_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNQ35699.1"
                     /translation="MRYEMMFPHQLRQALKQNRPAVLPVGVLEYHAEHCVLGVDTLLV
                     IRALELLEKELDLVLFPPFYYGAASYVVEPPENNGTVQVGAPALLEFARPLFASLLRV
                     GFRNIHLFIHHQSENFADGMPTDLAFKLAARQATFEFLEKQRGENWWGDPAMRDYYQR
                     NEAGANPFNWIRVHPFMSAEAQARYPIDHAGRQETSLMLAFCPEGVDDTRRRAAKKWY
                     SASAGQADLKYGRAARRLILDGMRRALQGAA"
     gene            2614..>4833
                     /locus_tag="PKN80_06500"
     CDS             2614..>4833
                     /locus_tag="PKN80_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNQ35700.1"
                     /translation="MLRILTAPFSCREGTRRLFELALAGENGPDFSGLWYIAPTPVKT
                     REARRLFNQSTGRAAFIPPRFYTPKQLANDLFENFGTANRFPDDLKPLLLAHLRTPLD
                     QPAENTNRFGFTRFLAGSIKEIKQYLPDQSPAALKERLPTLFDRSPDQLQKMLDNLDL
                     MAEYDRFLKRHDFLDSEDIQRAAPKLTDRLEPGPKTLLLDGFYDLTPVEANLLKALLR
                     RSERTIALAYHDPSEPEVYHIPNLFLEFLKDCAPFEICHLKPDPGPGRAAEYCELPDR
                     ETELELIAGLIKKAVLTEGVAPEKILVAFPDPAPYQKLAERTFARFGIPLRLEAEIPL
                     DQLAAAEPILSLLACLENDFPRRYLAALIGAPALPRLAPRLRDAFPRLSSRAGLNRGR
                     EAWRSLAWRLSRDPVAGDRLQEEGWSDQDLTAFQAELDQLLKVAAGLTRPRQDQLAGF
                     LSRLRALLTKFGWDEFLAGEPETVRESFYSLFNRLEDFGQRLLEDRKIDFVCFARMLR
                     HLLGQAAAGAESEGVAALSFLDTRGLSPERCFVAGLAEASLPRRPAFDPILPEFVKKA
                     LGLPERSGHLERERLHFRRLERLPAAGFFSFPRTSGDTLFLPSPFLLDRTRRFPDRPA
                     GLFSRAEVQAAAGRGKPVGRPDEPDLSADPVALEIIKARFGPNQRIRITWLDDYLRCP
                     ISFFFERVLRLERTSEPAYSVEPKVWGQLAHEVLEKLHQPGPAEPTGIPDRLVAVLTE
                     "
BASE COUNT          830 a         1763 c         1454 g          786 t
ORIGIN      
        1 catcgatctt gatgattcga aacagcacct gaaagccatt gatcaggatg gccacaccat
       61 cggcagcggc cttggcccac acagcaatac cgctttcatt gcccagctgg cgatcggcct
      121 tgaccagctc caggccggga aactccttcc gcagcttatc ctggatgaag gccggaaaga
      181 tgctgtggcc gatgatgccc agccgcttca gtttccttcc cgccataccc tcccggaaga
      241 cctcccggaa actggagacg ggaatggtcg ggtactccgg ctcggccgat tcccggtatt
      301 ccagcagcat cctgaacccg ggaatccggc tgtggctggc ggcaaaggtt tcgctctcgg
      361 ggccgatcag caggaccggc tctccctgcc ggggaatgaa gacgccggcg ctctcgaaga
      421 tcggccagta gtcggacagg tagcgcacgt tggcaaaatc ggcctcgttg gaatggacca
      481 gcgccgcgtc caggtccagt ttcgccaggc cggcctgcag ccgccgccag cgctcctgga
      541 actccgtccg gctgattccc tttgacatgc tcacctccgt ggttggcaag cggcgtggga
      601 accgcgtgtt atcaattccg gctcgaccag gatacgggtg ggccgggtcc cgcgcttctc
      661 gatccgctcc agcagcaccc gggccgcctc ggagccgatc ccgaagggct tctggtcgac
      721 ggtggtcagg ggcgggtcaa cgtagccgct ccagtgcagg ttgccgtacc cgatcacggt
      781 caggtcgccc ggaatgcgga tcttcatctc ccgggcccgc tggtagaccc cgatggccac
      841 ctcgtcgttg tgggcgaaga ccgcggtcgg cggcgcggcc agccccagca aatggccggc
      901 ctcccggtac ccgtcggccc gggtcacccc ggccagccga accagccgcc gatccgtctt
      961 gacgcccgcc gcctcgagga agtcccggca gcttttcatc cggcagtact cgggatctct
     1021 tagcgccgcg gccaggaagg caatccggcg gtggcccagc cgcagcagga gttcgccgac
     1081 cagccggccg cccgcctggt cgttggagta aacgtaggag cactccaggc cggggaagca
     1141 gcggtcgacg aagaccgccg gcagctccaa cttctgcagt gccaggtagg gctcgatgtc
     1201 gttgacgttg agggccgggg cgatgatcag tccgtccacc tgccgggaac gcaggacgcc
     1261 cagttcggcc cgctcccggg cggcatcctc gccggtgtgg cccaggatca ggtggtagcc
     1321 gttccggccg aaaacggtct cgatgccccg gatgatctcg aaaaagtagg agaggttgat
     1381 ggccgggacc agaaaaccga ccgtccggct ccgccggctg accaggctcc gggcgatgaa
     1441 attcggctga tagtcgtact ccttgaccag ctgcaggacc cgggcccgga ccacctccga
     1501 aacgtgggca tgctggttga ggacgcgcga aacggtcatg gccgacacgc ccgcccttgc
     1561 ggcgatatcc ttcaacctga ctcttctgct ggccatggcg gcctccggaa acgaccggcc
     1621 ggcggcgtct tgacactcgc tccggcctga tgctatgctt cagataacga taacacgacc
     1681 ggcgaaaacc tgtcaaaagg cggccagcgg ccgcggtttg cccctcccgg ccggccccgg
     1741 ccgcaaggag actcgatgcg ctacgaaatg atgtttcccc accagctgcg ccaggccctg
     1801 aagcagaacc gtccggccgt cctgccggtc ggcgtgctgg agtaccacgc cgagcactgc
     1861 gtcctgggcg tggacacgct cctggtaatc cgggccctgg agctcctgga aaaggagctg
     1921 gacctggtcc tcttcccgcc cttctactac ggcgcggcca gctacgtggt ggagccgccc
     1981 gaaaataacg gcaccgtcca ggtgggcgcc ccggccctgc tggaattcgc ccggccgctc
     2041 ttcgcctcgc tgctgcgggt cggcttccgg aacatccatc tcttcatcca ccaccagagc
     2101 gagaacttcg ccgacggcat gccgaccgac ctggccttca agctggccgc ccgccaggcc
     2161 accttcgagt tcctggagaa gcagcggggc gaaaactggt ggggcgaccc ggccatgcgc
     2221 gactactacc agcggaacga ggccggcgcc aatcccttca actggatccg ggtccacccg
     2281 ttcatgtcgg ccgaggccca ggcccgctac ccgatcgacc acgccggccg ccaggaaacc
     2341 tcgctgatgc tggccttctg cccggaggga gtcgacgaca cccggcgccg ggcggccaaa
     2401 aagtggtaca gtgcctcggc cggccaggcc gatctcaagt atggccgggc cgcccgtcgc
     2461 ctcatcctgg acgggatgag gcgggccctc cagggcgccg cctgaccgcg gaccccggct
     2521 tccttaacgc cgacgccgcc ggttccggac cggagatccg ccccggccgc cgcccgtttt
     2581 ttctggccgg gtttttacga taaaataata ctgatgctcc ggatactaac cgcgcccttc
     2641 tcctgccggg agggaacccg ccgcctcttc gagctcgccc tggccggcga aaacggcccc
     2701 gacttctccg gcctttggta catcgcgccc accccggtca aaacgcgcga agcccgacgc
     2761 ctcttcaacc aatcaaccgg ccgggccgcc ttcatcccgc cccggttcta cacccccaag
     2821 cagctggcca acgacctctt cgaaaacttc ggtacggcca accggttccc ggacgacctg
     2881 aaaccgctgc tcctggccca cctgcggact ccgctcgacc agcccgccga aaacaccaac
     2941 cgctttggct ttacccgatt cctggccgga agcatcaagg aaatcaagca gtacctgccc
     3001 gaccagtcgc cggccgcgct aaaggaaagg ctccccaccc tgttcgaccg gtcgccggac
     3061 cagttgcaaa aaatgctgga caacctcgac ctgatggccg aatacgaccg ttttctcaag
     3121 cggcacgact tcctggacag cgaggacatc cagcgggcgg ccccgaaact gaccgatcgc
     3181 ctcgaacccg gcccaaagac gctcctgctg gacggcttct atgacctgac gccggtagag
     3241 gccaacctgc tgaaagccct gctccgccgc tccgagcgga ccatcgccct ggcctatcac
     3301 gacccgtccg aaccggaggt ctaccacatc ccgaacctct tcctcgaatt cctgaaagat
     3361 tgcgccccct tcgaaatctg tcacctgaaa ccggatccgg gaccgggccg ggcggccgaa
     3421 tactgcgagt tgcccgaccg ggaaacggaa ctggaactca tcgccggtct gatcaaaaag
     3481 gcggtgctga ccgagggcgt ggccccggaa aagatcctgg tcgccttccc tgatccggcc
     3541 ccctatcaaa agctggcgga acgcaccttc gcccgtttcg gaatcccgct gcgcctggag
     3601 gctgagattc ccctcgatca actggcggcc gccgagccga tcctctccct gctggcctgt
     3661 ctggaaaacg acttcccgcg ccgttacctg gccgccctga tcggcgcgcc ggccctgccc
     3721 cgcctcgctc cccggctgcg cgacgccttt ccgcgtctct ccagccgggc cggcctcaac
     3781 cggggccgcg aggcctggcg cagcctggcc tggcgccttt ccagggaccc ggtggccggc
     3841 gaccgcttgc aggaagaggg ctggtcggac caggacctga ccgccttcca ggctgaactc
     3901 gaccagctgc tcaaagtcgc cgccggcctg acccggccgc gccaggacca actggccgga
     3961 tttctcagcc ggctccgtgc tctcctaacc aaattcggct gggacgaatt cctggccggg
     4021 gaaccggaaa cggtccgcga aagtttttac tccctcttca accggctgga ggacttcggc
     4081 caacgcctgc tcgaagaccg caagatagac ttcgtctgct tcgcccggat gctccgccat
     4141 ttgctgggac aagcagccgc cggcgccgag agcgagggcg tggcggcgct gagcttcctg
     4201 gacacccgcg gcctcagccc ggagcgctgc ttcgtggccg gcctggccga ggcctccctg
     4261 ccccggcggc cggccttcga cccgatcctg cccgaattcg tgaagaaggc gctcggcctg
     4321 ccggaacgct ccggccacct ggaacgggaa cgccttcact tccgccgcct ggaacggctt
     4381 ccggccgccg gcttcttctc cttcccccga accagcggcg ataccctctt cctcccctca
     4441 cccttcctgc tggaccggac ccgccgcttt ccggaccggc cggccggcct cttcagccgg
     4501 gccgaagtcc aggcggccgc cggccgcggt aaaccggtcg gacggccgga cgaacccgac
     4561 ctctcggctg acccggttgc cctggaaatc atcaaggcgc gtttcggacc gaaccagcgg
     4621 atccggatca cctggctcga cgattacctg cgctgcccga tcagcttctt ctttgagcgg
     4681 gtcctgagac tggaacgaac ctccgagcca gcgtattcgg tggagccgaa ggtctggggc
     4741 cagctggccc acgaagtcct ggagaaactc caccagcccg ggccggccga accgaccggc
     4801 attccggacc gcctggtcgc cgttctcacc gag
//
LOCUS       DAOQYN010000149         4810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_17964_length_4810_cov_4.351420, whole genome shotgun sequence.
ACCESSION   DAOQYN010000149 DAOQYN010000000
VERSION     DAOQYN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17964_length_4810_cov_4.351420"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKN80_06505"
     CDS             complement(<1..194)
                     /locus_tag="PKN80_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35701.1"
                     /translation="MKKKSLITVFSIGLVLLSGCVAMQEDVTNLDGRLISIQERLAQV
                     ETRLETVDRENRENRKIFEES"
     gene            complement(201..815)
                     /gene="pal"
                     /locus_tag="PKN80_06510"
     CDS             complement(201..815)
                     /gene="pal"
                     /locus_tag="PKN80_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNQ35702.1"
                     /translation="MRKWMMVLGMVVLAGILAGCACKVPSQKQITSVPPSTGTEVKPA
                     APGTPSTPVVPEVGRATPQEERIAMAGGDIPLKSPTVPENARPPRTEIEKQVFQIIHF
                     DFDKSDIRPDAQKILEGVADYLKKNPSVKIVVEGHCDEAGTEEYNLALGERRALAARR
                     YLVTLGIAPDRVTTLSFGESWPFDPEHNEKAWALNRRGEFKIVE"
     gene            complement(855..2066)
                     /locus_tag="PKN80_06515"
     CDS             complement(855..2066)
                     /locus_tag="PKN80_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35703.1"
                     /translation="MKNMKRAASLLILPLFFLLSAAGVGRGQVSLDITRQAPPRLPVG
                     LILPESLAEFNRVLEQDLRWSGFFETRRLPAGSDWPAGLSVLRVAAAESGLRVMLVAG
                     TPPVARLDRTYRLAASDERALAHRVAREVIEELTGQSAVTESRIAAVRQEGKARELVI
                     MDYDGANRKFLTSNGTLNLSPRFSPDGRSLIYTSFADYWPAIRRLTLAGGRVEKLAAY
                     PGLNSAAAFSPDGRRLVLALSKDGNPDLYLLDLAGGGLKRLTNHPANDTSPVFFPDGK
                     RLAFVSDRAGGPQIYRLELDSGRTERLTFEGGYNTSPDISPDGRFLAYTSRQGGQFEI
                     RILVLESGRHHTVTSGAGSKEDPAFASNSRHLVFVHTAKYRSELRLLDIFTGEDYKIT
                     EDGGYRTPDWR"
     gene            2533..3930
                     /locus_tag="PKN80_06520"
     CDS             2533..3930
                     /locus_tag="PKN80_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNQ35704.1"
                     /translation="MAKRLLTLTLAVVLGVAFVAPAMAEVQNIKVSGDMIMRGIYRDN
                     LDFVKDSGDETEAWISTGVRVGVGVEFTDNVMAMVRVINERDWGKSVIQTAAGAGTVV
                     GLPLDTSDDIKLDLAYAKINDLFIPGLNVTLGRQELLLGKGFVVGNVNSNLTAAANTA
                     IRAWDLTSRKSFDAIKAEYELGIAPLSFTVMGAKIIEESNNAVTALIPIPAGDSDLWG
                     LNVAYQMDNAVIEGYLLNLKRQAIPVLVTDKYDIWTLGARVDHDVAALPGFNYNLELG
                     YQFGDGADVNGDGTSDDKKAWAGTADVSYTFNNPYSPKIGAAYTYYSGPDFSATVADD
                     EGWDPIFPDGQAGRIGNVTNAVLSGTPAAFLAGIGNATNLSVPKIYASFKPGEKHALS
                     LDWYIDTQSTEVPAGTGDKLGWEVDLGYAYQLNSDVTMGLSIGYMEADDDFVGTGAGV
                     DDTAMEVIGSVSLAF"
     gene            complement(4091..>4810)
                     /locus_tag="PKN80_06525"
     CDS             complement(4091..>4810)
                     /locus_tag="PKN80_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HNQ35705.1"
                     /translation="AGEYPALCLAGVFSEAALYDLLEFRGASIIAGAAGRDLGTMLAA
                     AADAGRVSGIIEGLADVYPANLNSPRQTVLSGRADSLRLAAGRLKEQGIRSVPIPVSC
                     AFHSPFVQPARERLAARLAQVAFQAPRLPVYANALAAVYPSEPARVADILSGHLVSPV
                     RFVEEVEAMYRDGCTIFVESGPRSVLTGLVDQILEGRPHLAVAGNTGKGSDWFDLLNC
                     LARLAAAGIRIRLVALAERMA"
BASE COUNT          921 a         1495 c         1547 g          847 t
ORIGIN      
        1 gactcctcga agatcttccg attttcgcgg ttctcccggt caaccgtttc cagacgggtc
       61 tcaacctggg ccaatcgttc ctggatggat atcagccggc cgtccaggtt ggtgacatcc
      121 tcctgcatgg ccacacatcc ggagagaagg accaggccga tcgaaaaaac cgttatcagt
      181 gactttttct tcatcgccgg ttattcgaca atcttgaact cgccccggcg gttcaatgcc
      241 cacgcctttt cgttgtgctc cggatcgaag ggccagcttt cgccgaagct cagcgtggtt
      301 acccggtcgg gggcgatgcc cagggtgacc aggtaacgcc gggccgccag ggcgcggcgt
      361 tcgccgaggg ccaggttgta ctcctcggtg ccggcctcgt cgcaatgccc ctccaccacg
      421 atcttgaccg agggattctt cttcaggtaa tcggccacgc cctcaagtat cttctgggcg
      481 tcggggcgga tgtcgctctt atcgaaatca aagtggatga tctggaaaac ctgcttctcg
      541 atctccgtgc ggggcggccg ggcgttttcc ggaacggtcg ggctcttcag cgggatgtcg
      601 ccgccggcca tggcgatacg ctcctcctgc ggggtggccc ggccgacctc cggaaccacc
      661 ggggtcgagg gcgtgcccgg cgcggccggc ttgacctcgg tgccggttga gggcgggact
      721 gaagtgatct gcttctggct ggggaccttg caggcgcaac cggccagaat gccggccagc
      781 acaaccattc ccaaaaccat catccatttt ctcatcttgc tctcctcggt ttcgcgccgt
      841 tccccacgga aaaatcagcg ccagtcgggc gtccggtaac caccgtcctc ggtaatttta
      901 taatcttccc cggtaaagat gtcaagcagc cgcaactcac tccggtactt cgcggtatgc
      961 acgaaaacaa ggtggcggct gttcgaagca aaggccgggt cctccttgct gccggccccg
     1021 gaagtcacgg tgtggtgccg gccactttcc agaaccagga tcctgatctc gaactgcccg
     1081 ccctggcgac tggtataggc caggaagcgg ccgtcgggcg aaatgtcggg agaggtgttg
     1141 tacccgccct cgaaagtgag ccgctcggtc cggccgctgt ccagttcgag ccggtagatc
     1201 tgggggccgc cggcccgatc ggatacgaag gccaggcgct tcccgtccgg aaagaagacc
     1261 ggcgaagtgt cgttggccgg gtggttggtc agccgcttca agccgccgcc ggccaggtcc
     1321 agcaggtaaa ggtcgggatt gccgtccttg ctcagggcca ggaccaggcg gcgaccatcc
     1381 gggctgaagg cggcggcgct gttgaggccg ggataggcgg ccagcttttc cacgcggccg
     1441 ccggcgagag tcaggcgccg gatcgccggc cagtagtcgg cgaaggaggt atagataaga
     1501 gagcggccgt cgggcgagaa gcggggcgac aggttgagtg ttccgttgct ggtcagaaac
     1561 ttgcggttgg cgccgtcgta atccatgatg accagctccc gcgccttgcc ttcctggcgc
     1621 acggcggcaa tccggctctc ggtcacggcc gactggccgg taagttcctc gatgacttcc
     1681 cgggccaccc gatgggccag ggcacgctca tccgaagcgg ccaggcggta ggtccggtcc
     1741 agccgggcca ccggcggcgt cccggccacc agcataaccc ggagtccaga ctcggcggcc
     1801 gccactcgga gcaccgaaag gccggccggc cagtcgctgc cggccggcag gcgccgggtt
     1861 tcaaagaagc cgctccagcg caggtcctgc tccaggaccc ggttgaactc cgccagcgat
     1921 tccggcagga tcaaaccgac cggaaggcgg ggcggcgcct ggcgggtgat gtcgagcgac
     1981 acctggccgc ggccgacacc ggcggccgat aaaagaaaaa aaagaggaag aatcaaaaga
     2041 gaagccgcgc gtttcatgtt tttcattata gcccataatc agaagggcgt caatccttga
     2101 taaaacaagg cacaccccct tttttacaag cattgacggg cgttccggcg cccaagcgcg
     2161 gtcaaccttc tcctggtatt tatcctaatt atcaggcttt tactttggat atgtcccggg
     2221 gaaccggtcc cctcttgaca gaagtcgttg ccccgttata taatgatggg caaaatgtga
     2281 attccgcctt ggtctcgacg gaaggatcag gaaaataaaa gccggaaagg aggtgattct
     2341 tcaagccgct ttcgtgggcg gcgcaggtta ttcccccttc gcctgggaga cgcaaggaat
     2401 gccgacaact ccccgggggt accggaaagt acaaccggtc aaataggaag tcaagcgagt
     2461 tgaatggctg tgctgtaaac tgtttgacag ccgcagagaa agttatcggg aagtaaaacg
     2521 gaggaaaacg aaatggcaaa acgcttactc accctgaccc tggccgttgt gctgggcgtg
     2581 gcttttgtcg cgccggccat ggccgaggtt cagaacatca aggtctccgg tgacatgatc
     2641 atgcgcggca tctaccggga caaccttgat ttcgtgaagg acagcggcga cgagaccgag
     2701 gcctggattt ccacgggcgt ccgggtcggc gtcggtgtcg agttcaccga caacgtgatg
     2761 gcgatggtgc gggtcatcaa cgagcgcgac tggggcaaga gcgtcatcca gaccgcggct
     2821 ggcgcgggca cggtggtcgg cctgccgctg gacacttcgg atgacatcaa gctcgacctg
     2881 gcctacgcga agatcaatga cctgttcatc cctggcctga acgtgaccct gggccgccag
     2941 gaactgctcc tgggcaaggg tttcgtggtc ggcaatgtca acagcaacct gacggcggct
     3001 gccaacacgg ccatccgggc gtgggacctg accagccgga agtcgtttga cgcgatcaag
     3061 gccgagtatg agctcggaat tgctccgctc agcttcaccg tcatgggcgc gaagatcatc
     3121 gaagagtcga acaacgcggt tacggcgctc atcccgatcc cggccggtga ttccgacctg
     3181 tggggcctga acgtggccta ccagatggac aacgccgtga tcgagggtta cctgctgaac
     3241 ctcaagcgcc aggcgattcc ggtgcttgtg actgacaagt acgacatctg gacgctgggc
     3301 gcccgggtgg atcacgatgt ggccgctctg ccgggtttca actacaacct ggagcttggt
     3361 tatcagttcg gtgacggtgc ggatgtcaac ggtgacggca cctccgacga taagaaagcc
     3421 tgggccggca cggccgatgt cagctacacc ttcaacaacc cgtacagccc gaagatcggc
     3481 gccgcctata cctattacag cggcccggac ttcagcgcga cggtcgccga cgatgagggt
     3541 tgggatccca tcttcccgga tgggcaggcc ggccgcatcg gtaacgtgac caacgcggtg
     3601 ttgagcggaa ctccggccgc cttcctggcg ggaatcggaa acgccaccaa cctgtcggtc
     3661 ccgaagatct acgcgtcctt caagcccggc gagaagcacg ccctcagcct ggactggtac
     3721 attgacactc agtccaccga agttccggcc ggcactggcg acaagctcgg ctgggaagtc
     3781 gacctgggtt atgcgtacca gctcaactcc gatgtgacca tgggcctttc catcggttac
     3841 atggaggctg atgatgattt cgtcggtacc ggtgccggcg ttgacgacac cgcgatggaa
     3901 gtcatcggct cggtttcgct ggccttctaa acaggtccgc gaaaggcatt aagaagcccc
     3961 ggctgccgaa aagcagccgg ggctttcttt tttgcgtgag accacccgcc ggacccgcaa
     4021 gttaccaacc taaagccaac ctctcaggct cccgccggtt ccagccggcc gccaccagca
     4081 gtcccccgga tcaggccatc ctttccgcca gggcgaccag cctgatccgg atcccggcgg
     4141 cggccaggcg ggccaggcag ttaagcaggt caaaccagtc gctccccttg ccggtgttgc
     4201 cggccacggc caggtgggga cgcccctcca gtatctggtc gaccaggccg gtcaggacgc
     4261 tgcggggacc ggactccacg aagatggtgc agccgtcccg gtacatggcc tcgacctcct
     4321 cgacaaaacg gaccgggctg accaggtggc cggagagaat gtcggccacc cgggccggct
     4381 cggacgggta gacggccgcc agcgcgttgg cgtaaaccgg caggcgcggc gcctgaaaag
     4441 cgacctgggc cagccttgcc gccaggcgct cccgggccgg ctggacgaag ggagaatgga
     4501 aggcgcagct caccggaatg gggaccgagc ggatgccctg ctccttgagg cggccggcgg
     4561 ccagccggag cgaatcggcg cggccggaaa ggaccgtctg gcggggggag ttcaggttgg
     4621 ccgggtagac gtcagccagt ccctcgatga tgccggagac ccgcccggcg tcggcggcgg
     4681 ccgccagcat cgtgcccaga tcgcggccgg cggcgccggc gatgatgctg gcgccccgga
     4741 actccagcag gtcataaagc gccgcctccg aaaagacccc ggccaggcag agggccgggt
     4801 attcgccggc
//
LOCUS       DAOQYN010000150         4799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_18026_length_4799_cov_4.563238, whole genome shotgun sequence.
ACCESSION   DAOQYN010000150 DAOQYN010000000
VERSION     DAOQYN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18026_length_4799_cov_4.563238"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="PKN80_06530"
     CDS             <1..1855
                     /locus_tag="PKN80_06530"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNQ35706.1"
                     /translation="RVDLLLRYRHPASKLEQLQYRAIHAAEQYAMEYEARIESLGGIG
                     FFLGGIGPDGHIGFNIRGSDHYSTTRLTPINYETAAAAATDLGGLENARDRVVMTVGL
                     RSITRNRTAAVLVTAAGESKAAVVRNAVENPPSVLYPATALQGLPGARFYLTQGAARD
                     LVERRCQALVQEKPLSDRSVERILIDLSSRRRKRLLDLTPADLEGDRIGRVLGGRFEG
                     AGALKEQAVRVEALLREKVARGLEEKEGLTFLHTAPHHDDIMLGYLPYIIRLVRSPKN
                     THYFATLTSGFTSVTNDYTLSALSNLRRFLERRTFAPLLAAGYFAPGRTPARNEDVYY
                     YLDGVAADSPEMRREAEARRALRVLLEMTGAADQAAAAAALEGLEQELRGSYPGKKDI
                     PAIQQIKGTIREWEEELLWGHLGFNSDNIFHLRLGFYTGDIFTPQPELERDVRPVLKL
                     LRELKPDVITLALDPEGSGPDTHYKVLQAVAEAVRVYRAETPQKDLKIWGYRNVWYRF
                     HPSEANIFAPVSMNSLAIMKEAFHTCFGSQRSASFPSYEYDGPFCDLARRIMVEQYTM
                     VMHCLGRDFFYKNPVPRLRASRGLNFIREMDCEEFSQEALSLRRLTEAQPF"
     gene            1928..3058
                     /locus_tag="PKN80_06535"
     CDS             1928..3058
                     /locus_tag="PKN80_06535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNQ35707.1"
                     /translation="MSSRFYWYLPTEVFFGVSALDVLSEKAAGLGRKALLVTGRSFAA
                     KSGLRTRIEERLKAAGVAWAVFDGAIPDPEIGTVAAGAEVCRRENCNFVIGAGGGSAM
                     DVAKGIAAAAPDPARPIEELFGYEQLRVRPLPVAAVPTTSGSGSEVTRYAVIVDPAAR
                     TKKTISSASINPRLALVQPELTLTLPADLTAETGLDAFCHAVEGSLAAARTPVSLALA
                     REAVRLITRHLPELVRRPDDLAGREGMSLAALTAGMVINQTGTIIGHGMGYPLTINYG
                     IQHGLASVMAMPAILDELIVTDEYGPVIREVTGWTEPGRQLRDFCGRLGRPASLKAAG
                     IGRSDLPKLAAEAVVGCQRAMQNMGRNFTEADFLRFYESVYD"
     gene            3088..4005
                     /gene="ribF"
                     /locus_tag="PKN80_06540"
     CDS             3088..4005
                     /gene="ribF"
                     /locus_tag="PKN80_06540"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNQ35708.1"
                     /translation="MEVIPLSGLDGLKDTVATIGTFDGIHLGHRAILEQVTGDARRLP
                     GRSLLLTFMDHPRILLKREPVFLINSPEIRREILAGLGLDYTVIIDQESVFDFSAEEF
                     VERILVGRLGVREVVIGFNFRFGRKRTGDAGLLAEWGGRCGFRVKVVKPVSLDGAVVS
                     SSRVRDRLAAGRVEEADRLLGRPYQVRGRVVPGRGRGREIGYPTANLEPDVPILLGTG
                     IYAVEVQALDAAAGLSGRVRALASYGHNPTFGRLEKPVLEIHIPGFSGELAGRRLAAD
                     FRRRLRSEAAFAGPEELAAAIRRDLESLN"
     gene            4047..4769
                     /locus_tag="PKN80_06545"
                     /pseudo
     CDS             4047..4769
                     /locus_tag="PKN80_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
BASE COUNT          763 a         1611 c         1621 g          804 t
ORIGIN      
        1 ccgggtcgat cttttgctgc ggtaccgcca tccggcctcc aagctggagc agctgcagta
       61 ccgggccatc cacgccgccg agcagtacgc catggagtac gaggcccgga tcgagtcgct
      121 gggcggcatc ggcttctttc tgggcggcat cggccccgat ggccacatcg gcttcaacat
      181 ccggggttcg gaccattaca gcaccacccg gctgaccccg atcaactacg agacggccgc
      241 ggccgccgcg accgacctgg gcggcctgga gaatgcccgg gaccgggtgg tgatgaccgt
      301 cggcctgcgg agcatcaccc ggaaccggac ggccgccgtc ctggtcaccg ccgccggcga
      361 gagcaaggcg gccgtggtcc ggaacgcggt tgaaaatccg ccctcggtgc tctatccggc
      421 caccgcgctc cagggactgc ccggcgcccg tttctacctt acgcaggggg cggcccggga
      481 cctggtcgag cgccgctgcc aggccctggt tcaggagaag ccgttgtcgg accggtccgt
      541 cgagcgcatc ctgatcgatc tcagctcccg ccggcgcaag cgcctgctcg acttgacgcc
      601 ggccgatctc gaaggcgacc ggatcgggcg ggtgctcggg gggcggttcg agggagccgg
      661 cgccctgaag gagcaggccg tccgggtcga ggcgctgctc agggagaagg tggcccgcgg
      721 cctggaggag aaggagggac tgaccttcct gcacaccgcg ccccaccacg acgatatcat
      781 gctgggctac ctgccctaca tcatccggct ggtgcgcagt ccgaagaaca cccactattt
      841 tgccacgctg accagcggct tcacctcggt gaccaacgac tacacccttt cggccctgtc
      901 taatctccgg cgcttcctgg aacgccggac cttcgcccct ctgctggccg ccggctactt
      961 cgcgcccggc cggaccccgg cccgcaacga ggacgtctac tattacctgg acggcgtggc
     1021 cgccgacagc ccggagatgc gccgggaggc cgaggcgcgc cgcgccctgc gtgttctgct
     1081 ggagatgacc ggcgccgcgg atcaggcggc ggccgccgcg gccctggagg gcctggagca
     1141 ggagttgcgc ggttcctatc ccggcaagaa ggacatcccg gccatccagc agatcaaggg
     1201 gacgatccgg gagtgggagg aggagctgct ctggggtcac ctgggcttta acagcgacaa
     1261 catcttccac ctgcgcctcg gtttttacac cggcgatatc ttcaccccgc agccggagct
     1321 ggaacgggac gtccggccgg tcctgaagct gctgcgcgag cttaagccgg acgtgatcac
     1381 cctggcgctc gacccggagg gcagcggtcc ggacacccat tacaaggtgc tccaggcggt
     1441 cgccgaagcg gtccgggtct accgggccga aacgccgcaa aaggatttga agatctgggg
     1501 ttaccgtaac gtctggtacc gttttcatcc ctccgaggcc aatatcttcg cgccggtctc
     1561 catgaattcg ctggccatca tgaaggaggc cttccacacc tgcttcggct cccagcgcag
     1621 cgcctccttc cccagctacg agtatgacgg acccttctgc gatctggccc gacggatcat
     1681 ggtcgagcag tacaccatgg tcatgcactg cctcggccgg gattttttct acaagaaccc
     1741 ggtgccgcgc ctgcgcgcca gccgcggatt aaacttcatc cgggagatgg attgcgagga
     1801 gttttcccag gaggccctct ccctgcgccg cctcaccgag gcccagccct tctgatccct
     1861 gcccgcccgg tggcgcggcc ggggcggcgc cttccaggtt ccgccgtttc tgtagtataa
     1921 tataaagatg tcttcccgtt tttactggta cctgccgacc gaggttttct tcggcgtctc
     1981 cgccctcgac gtcctttccg aaaaggccgc cgggctgggc cgcaaagccc tgctggtcac
     2041 cggccggagt tttgccgcaa agagcggcct gcggacccgg attgaagagc ggctcaaggc
     2101 ggccggggtc gcctgggcgg tcttcgacgg cgcgattccc gatccggaga tcggcaccgt
     2161 ggccgccggg gccgaggtct gccggcgcga aaactgcaat ttcgtgatcg gcgccggcgg
     2221 cggcagcgcc atggacgtgg ccaagggcat cgcggcggcg gcccccgacc cagcccggcc
     2281 gatcgaagaa cttttcggct acgaacagct gagggttcgg cctctgccgg tggcggccgt
     2341 tccgaccacc tccggcagcg gcagcgaggt cacccgctac gcggtcatcg tcgacccggc
     2401 ggcccggacc aagaagacca tcagctccgc ctcgatcaat ccccggctgg ccctggtcca
     2461 gccggagtta accctgaccc tgccggccga tttgaccgcc gagaccggcc tggacgcctt
     2521 ctgccacgcg gtggagggct cgctggccgc cgcccgcacc ccggtcagcc tggcgctggc
     2581 ccgggaggcg gtccggttga tcacgcgcca cctgccggaa ctggtgcgcc ggcctgatga
     2641 cctggccggc cgggagggaa tgagcctggc ggccctgacc gccgggatgg tcatcaacca
     2701 gaccggcacc atcatcggcc acggcatggg ttaccccctg accatcaatt acggcatcca
     2761 gcacggcctg gccagtgtca tggcgatgcc ggccatcctg gacgagctga ttgtcaccga
     2821 cgagtacggc ccggtgatcc gcgaggtcac cggctggacg gaacccggcc ggcagctgcg
     2881 cgatttctgc ggccgcctcg gccggccggc cagcctgaag gccgccggta tcggccggtc
     2941 cgatcttccg aaactggccg ccgaggcggt ggtgggctgc cagcgagcca tgcagaacat
     3001 ggggcggaac ttcaccgagg ccgatttcct gcgtttttat gagtccgttt acgattgagg
     3061 agcggccggc cgtccggcgt cccggctatg gaagtcatac cactgagcgg attggatggg
     3121 ctgaaggata cggtggccac catcggcacc ttcgacggca tccatctcgg ccaccgcgcc
     3181 atcctggagc aggtgaccgg ggatgcccgg cgcctgccgg gtcggagcct gctgctcacc
     3241 ttcatggatc acccgcgcat cctgctcaag cgcgaaccgg tttttttgat caactccccg
     3301 gagatccgcc gggagatcct ggccggcctg gggcttgatt atacggtcat catcgaccag
     3361 gagtcggtct ttgatttttc ggccgaggag ttcgtggagc ggatcctggt tggccgcctg
     3421 ggcgtaaggg aagtggtgat cggtttcaac ttccgcttcg gacggaaacg aaccggcgac
     3481 gccggtctgc tggcggagtg gggcggccgg tgcggttttc gggtcaaggt cgtcaagccg
     3541 gtcagcctgg acggcgcggt ggtcagcagt agtcgcgtgc gcgaccgcct ggccgccggc
     3601 cgggtcgagg aggccgaccg gctcctggga cgcccctacc aggttcgggg acgggtggtt
     3661 cccggccggg ggcgcggccg cgaaatcggc tacccgaccg ccaacctgga gcccgacgtt
     3721 ccgattctgc tcgggaccgg catttacgcg gtcgaggttc aagccctgga cgcggccgcc
     3781 ggcctttccg gccgggttcg ggccctggcc agttacggcc acaacccgac tttcggccgc
     3841 ctcgagaagc cggtgctgga gattcacatc cccgggtttt ccggggaact ggccgggcgg
     3901 cggctggcag ccgatttccg gcgccgcctg cggtcggagg cggcgtttgc cgggccggag
     3961 gagctggccg cggccatccg gcgcgatctg gagagcttaa actgatttac tgatcaggtt
     4021 ttcttaaccc agaggaggaa ggacttatgg cgaagcagaa attcgtttac tatttcggcg
     4081 acggcaaggc cgacggccgg agcgacatga aggagctgct gggcggcaag ggggccaatc
     4141 tggccgagat ggtcaacctg ggcatcccgg ttccggccgg tttcaccatc accaccgagg
     4201 tctgcaccca ttattaccgg aaccgccgcc agcttcccga cggagtggaa gcggatatcg
     4261 accgggccct ggcccgggtc gaaaagtcga tgggctaccg cttcggcgac ccggtcaacc
     4321 cgctgctggt ttcggtccgc agcggcgcgc gccgttccat gccgggcatg atggagacgg
     4381 tactcaacgt cggcctctgc gcccggacca ttcccggcct ggtcaagaag accggcaacc
     4441 cgcgtttcgt ttacgacgcc taccggcgcc tgatcatgat gtactcggac gtggtcatgg
     4501 agaaggcggc cggcgtcgag cccgaagagg ggcgggggat ccgggttcag ctcgaggggc
     4561 tgctcaagga gctcaaggcg gcccgcggct acaaggcgga taccgacctg accgctgagg
     4621 agctgaagga cctctgcgaa cgcttcaagg aacgggtcaa gaaggttttg ggccgggcct
     4681 tccccgacga tccgcgcgag cagctctacg gcggcatcaa ggcggtcttc gcctcctgga
     4741 acggccgccg ggccgtctcg atccggcggt aggagacggc ccggcggccg ttccaggag
//
LOCUS       DAOQYN010000151         4789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_18060_length_4789_cov_4.307562, whole genome shotgun sequence.
ACCESSION   DAOQYN010000151 DAOQYN010000000
VERSION     DAOQYN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18060_length_4789_cov_4.307562"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PKN80_06550"
     CDS             <1..532
                     /locus_tag="PKN80_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35709.1"
                     /translation="IRRMKAIRQWRWARNPAAADRGRFQRPGYFFADDAIALISARQY
                     REFVFPYHKRLVEAFSDGGPVGIHLCGDATRHFKFLKENLNVRTFDTGFPVDFGGLRR
                     ELGPEVQINGGPTVMLLKDGSPEEVRDEVRRICRSGIMEGGRFVLREANNLAPATPVE
                     NVAAMYEAGREFGRYG"
     gene            572..1219
                     /locus_tag="PKN80_06555"
     CDS             572..1219
                     /locus_tag="PKN80_06555"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNQ35710.1"
                     /translation="MKKEPGFTLIELLVVMAIIAILAALLLPALSAAREKGRQASCLN
                     NLKQLYLALEFYAEDYNELYPRACGTVRWDELDPDDGTYGWMQQLFPYARNKRIYKCP
                     SDRDSDYSYFLGARAAYLAAGGRASVQRKRILYPSAYVLAGDTRDFSHDECDKDDYSQ
                     NCVGGAEHASPYVAKWRSHSGGQNILFADGHCRWYRGYDPAEMTFRYDIMHSWAE"
     gene            1326..3110
                     /locus_tag="PKN80_06560"
     CDS             1326..3110
                     /locus_tag="PKN80_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35711.1"
                     /translation="MKRWIFLCLSVGVGWFFGAGTAWTLDRPAWMDEPGIVMAGNWEE
                     PTFRARRMGRLDFTLPPERLEGYEREHSPEMVAELKELGVNFLMIHCYKGAGLETERQ
                     GMEDAKKLAALARQAGMRVGTYIGGTMLYERLFKEEPNAPQWQAFGPAGEPLYYSSSQ
                     KFRYAAVRNHPDFIEYLKKPVRFAIEEVKADLIHFDNFGLGSASYDPVSKQQFRAFLK
                     ARGMAEAEPPASIANPNDPLVRAWLDFKCQALADHYAAMSKYIRSLNPRCGVESNPGS
                     VLAAGRSRTGVDHARLMPHGNASWDEARSTAWDPKSRMARTRIRSLKVGRLFNNSTFL
                     YTETPLDAAESMAFNLNCLGSVAWFEWGRVRSAHLNDQPIHPELKTYIRFFLDRPDLF
                     RCSKPVADVAVLRTFAETNFGSSAENAKRNNIEQGLIQGSTAWRIIFDGQLDALDDYR
                     VVIVPGKTWLTAAQQKALDDFATRGGLVMPAMSVANPAETAVLLRDKLRLVVEAPSSV
                     AVELCEQASPKRVLIHFVNYNAEETLENVQVKIRFKSGRPSRVRLLSPDPAGDKSLKI
                     RGKDGQYSFTLPKLKIYAVAVIEGASVQ"
     gene            3160..4059
                     /locus_tag="PKN80_06565"
     CDS             3160..4059
                     /locus_tag="PKN80_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ35712.1"
                     /translation="MRKGKQILWLAVYGVGGSLVVVFQRYLTFHFDNLTQNAYRFLAG
                     SAALFLLAHHFFRAELVRLLKDRRQILRIFGSALLLTAPTFLIVEGIARTTAALAGLL
                     HTMAIPLTLLLALALFPDERNLGQPGFFLAGTLAALAGTVGLTLSGGSLAFQRSIGNL
                     YVLAATLVGVACGLIIKRLVRDTQPVCLGAFTCGFVGLLFLAVLAVQGRMTAFLGAGF
                     LPVTVLFFSGVYGMLIGVGLAYTLIRKAGLITVRLTDLAMPVFTALFGFLLFGERLAR
                     TQMLYGGLVLGGCLMVLLNRPHR"
     gene            complement(4067..>4789)
                     /locus_tag="PKN80_06570"
     CDS             complement(4067..>4789)
                     /locus_tag="PKN80_06570"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNQ35713.1"
                     /translation="VHEVAQNIAAHPLDPIIIQGYIYDITDRRAAEGALTRAKTELTA
                     SLDQLQSLFSGIVEALASTVELRDPYTSGHEKRVTLLACALAREIHFDENRIAGLRIA
                     GLLHDIGKLMVPAEILNKPTILSETEYDFIRIHPQAGHDILRKIAFPWPVADIVLQHH
                     ERLDGSGYPQGLKAPEIILEARILAVADVMEAMVSHRPYRPAKNLDRALKEIREEKAG
                     LFDQRVVKSCAGLFEEKQFRFD"
BASE COUNT          821 a         1488 c         1556 g          924 t
ORIGIN      
        1 catccgtcgg atgaaggcca tcaggcagtg gcgctgggcg cggaatccgg ccgccgccga
       61 ccggggccgc tttcaacggc ccggatactt cttcgccgac gacgcgatcg ccctgatctc
      121 cgcccggcag taccgggagt tcgtctttcc ataccacaag cggctggtgg aggccttctc
      181 cgacggcggt ccggtcggca tccacctctg cggagacgcc acccgccact tcaagtttct
      241 caaggagaac ctgaacgtcc gcaccttcga caccggtttc ccggtcgatt ttgggggcct
      301 gcggcgggaa ctggggccgg aggtccagat aaacggcgga ccgaccgtca tgctcttgaa
      361 ggacggcagc ccggaggagg tccgggacga ggtgcggcgg atctgccgct ccggcatcat
      421 ggagggcggc cggtttgtgc tgcgggaggc caacaacctg gctcccgcca cgccggtcga
      481 gaatgtcgcg gccatgtacg aggccggtcg ggagttcggc cggtacggtt gaaaaggttt
      541 cggaagggag agccggggag ttgaaaacga catgaaaaag gagccgggct ttactctgat
      601 cgagctgctg gtggtgatgg ccatcatcgc catcctggcc gccctgctgc tgccggccct
      661 ctcggcggcc cgggagaagg ggcgccaggc ctcctgcctg aacaacctga aacagcttta
      721 cctggccctg gagttctacg cggaggatta caacgaactc tacccgcgcg cctgcgggac
      781 cgtccggtgg gatgaactgg atcccgatga cggaacctac ggctggatgc agcagctctt
      841 tccctacgcc aggaacaagc ggatctacaa gtgtccctcg gaccgcgact cggattacag
      901 ctacttcctc ggtgcccggg ccgcctacct cgccgccggc ggccgggcca gcgtccagcg
      961 taagcggatt ctctacccga gcgcctacgt cctggccggg gatacccgcg atttttccca
     1021 cgatgagtgc gacaaggacg attactccca gaactgcgtg ggcggggcgg aacacgccag
     1081 tccctacgtg gctaagtggc ggtcgcactc gggcggtcag aacatcctct ttgccgacgg
     1141 ccactgccgc tggtaccggg gttacgatcc ggccgagatg acctttcgct acgacatcat
     1201 gcacagctgg gccgagtgag aaaggcggtt gggtagagag agaaagagat caacttgaga
     1261 aaataacccg cccaagtcga aattcgatac caggcggccg ttccaatctc aaaaggaagt
     1321 ctgaaatgaa acgatggatt tttttgtgcc tttcagttgg agtggggtgg tttttcggcg
     1381 cgggcacggc ctggacgctt gatcgtccgg cctggatgga tgagccgggt atcgtgatgg
     1441 cgggcaactg ggaggagccg acgtttcgcg cccggcggat gggacggctg gatttcacgc
     1501 tgccgccgga gcggcttgaa ggatacgagc gggaacacag cccggagatg gtcgcggagt
     1561 tgaaggaact cggcgtcaac ttcctgatga tccactgtta caagggcgcg ggcctggaga
     1621 ccgagcgcca ggggatggag gacgcgaaga aattggcggc gctggctcgt caggccggta
     1681 tgcgcgtcgg cacctatatc ggcggcacga tgctttacga acgccttttc aaggaggagc
     1741 cgaacgctcc ccaatggcag gcattcgggc ccgcgggcga accgctttac tacagttcct
     1801 cccagaagtt ccgctacgcc gcggtgcgta accacccgga ttttatcgaa tacctgaaga
     1861 aaccggtccg cttcgccatc gaagaggtca aggccgacct gattcacttc gacaacttcg
     1921 gactcggcag cgcctcgtac gacccggtct ccaagcagca gttccgggcc tttctcaagg
     1981 cgcgcgggat ggccgaggcc gagccgccgg caagcatcgc caatccgaac gatcccctgg
     2041 tgcgcgcctg gctagatttc aaatgccagg cgctggccga ccattacgcg gcaatgtcga
     2101 aatacatccg ttccctcaat ccccggtgcg gcgtggaatc gaaccccggt tccgtcctcg
     2161 cggccggccg ctcgaggacc ggcgtcgatc acgcccggtt gatgccgcac ggaaacgcct
     2221 cctgggacga ggcgaggagc acggcctggg atccgaagag ccggatggcg cgcacccgaa
     2281 ttcgttcact caaggtcgga cggcttttca acaactccac cttcctctat accgagacgc
     2341 cgctggatgc cgccgagagc atggccttca atctcaattg cctcggttcg gtggcctggt
     2401 tcgagtgggg ccgggtccgg agcgcgcacc tgaacgacca gccgatccat ccggagttga
     2461 agacctacat ccgattcttt ctcgaccggc cggacctgtt ccggtgctca aaaccggtgg
     2521 ccgacgtggc ggtgctgcgg accttcgccg agaccaactt cggatcctct gccgaaaacg
     2581 cgaagcgcaa caatatcgaa cagggtttga tccagggttc gaccgcctgg cgcatcatct
     2641 tcgacggaca gctggacgcc ctcgacgact accgggtcgt catcgttccc gggaagacgt
     2701 ggttgaccgc ggctcagcag aaggcgcttg atgatttcgc gacccggggc ggcctggtga
     2761 tgcccgcgat gagtgtcgcg aatcccgccg aaacggcggt cctgctgcgt gacaagctcc
     2821 ggctcgtcgt cgaggcgccg tcgtcggtgg ccgttgaact ttgcgagcag gcgtctccga
     2881 agcgggttct gatccatttc gtcaactaca atgcggagga gacgctcgaa aacgtccagg
     2941 tcaaaatccg gttcaagtcg ggccggccct cccgggtccg actactgtca ccggatcccg
     3001 ccggcgataa gagcctcaag atccggggaa aagacggtca atactctttt acgctgccga
     3061 aactcaagat ctatgccgtg gccgtcatcg agggcgcctc ggtgcaatga gcaatcggcc
     3121 ggtcgggacc gccggcgtgt tacactatcg cctattaaca tgcgcaaggg caagcagata
     3181 ctctggctgg cggtgtacgg ggtgggcggg tcgctggtgg tggtcttcca gcgctacctg
     3241 actttccact tcgataacct gacccagaac gcctaccgtt tcctggccgg ctcggcggcc
     3301 ctcttcctgc tggcgcacca tttcttccga gccgaactgg tcaggctcct gaaggaccgg
     3361 cggcagatcc tgagaatctt cggctccgcc ctgctgctca cggcgccgac ctttctgatc
     3421 gtggagggca tcgcccggac caccgcggct ctggccgggc tgcttcacac gatggccatt
     3481 ccgctcaccc tgctgctggc tctggccctc ttcccggacg aacggaacct gggccagccc
     3541 ggtttctttc tcgccggcac gctggcggcc ctggccggca cggtcggcct gaccctctcc
     3601 ggcgggtcgt tggcctttca acgttcgatc ggcaacctct acgtcctggc cgccaccctg
     3661 gtcggggttg cctgcgggct gatcatcaag cggctggtgc gcgataccca gccggtctgc
     3721 ctgggcgcct tcacctgcgg gttcgtcggt cttctgtttc tggcggtgct ggccgtccag
     3781 ggacggatga cggcttttct gggggcgggt tttctcccgg tgacggttct cttcttctcc
     3841 ggggtttacg gcatgctgat cggggtcgga ctggcctaca ccctgatccg gaaggctggt
     3901 ctgatcacgg tgcggctgac cgacctggcc atgccggtct tcacggccct cttcggcttc
     3961 ctgctcttcg gcgaacggtt ggcccgaacg cagatgctct atggcggact ggtcctgggc
     4021 ggttgtttaa tggtgctgct gaaccggccg caccgctgaa gccgggtcaa tcaaaccgga
     4081 actgcttctc ctcgaagagg ccggcgcacg acttgaccac gcgctggtcg aagagcccgg
     4141 ctttttcttc ccttatctcc ttcagggccc ggtcgagatt cttggccggc cggtagggtc
     4201 ggtgcgagac catggcctcc atcacgtcgg ccacggccag gatccgcgcc tcgagaatga
     4261 tctccggtgc cttgagtccc tgtgggtagc cggagccgtc cagccgctca tggtgctgca
     4321 ggacgatgtc ggccaccggc cagggaaagg cgatcttgcg caggatgtcg tggccggcct
     4381 gggggtggat gcgtatgaaa tcgtactcgg tctccgaaag gatggtcggc ttgttcagga
     4441 tctcggccgg gaccatcagc ttgccgatgt cgtgcaggag gccggcgatc cggaggccgg
     4501 cgatccggtt ttcgtcgaag tgtatttcac gggccagggc gcaggccagg agggtcactc
     4561 gtttttcatg gccggaggtg tagggatcgc gcagttcaac ggtcgaggcc agcgcctcca
     4621 cgattccgct gaagaggctc tggagctggt ccaggctggc ggtgagttcg gtcttggccc
     4681 gggtcagtgc cccctcggcg gcccgccggt cggtgatatc gtagatgtat ccctggatga
     4741 tgatcgggtc caggggatgg gcggcgatgt tctgggcgac ctcgtgcac
//
LOCUS       DAOQYN010000152         4754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_18231_length_4754_cov_3.714407, whole genome shotgun sequence.
ACCESSION   DAOQYN010000152 DAOQYN010000000
VERSION     DAOQYN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18231_length_4754_cov_3.714407"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..730
                     /locus_tag="PKN80_06575"
     CDS             74..730
                     /locus_tag="PKN80_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35714.1"
                     /translation="MADLERVLETFKSKLLEIWGDDLQSLLLYGSYLGPDFQPGHSDL
                     NLMAVVRRDDPEKLFRSRRLVASFHRRRRLVPLFFTAEFLKESLDTFPVEFEAIRRRY
                     RVLHGSDPVAGLDLSKADLRLQLEREIKENIIRFRQGILFGWEIPSLLGSSVKSMRIL
                     LEAARLAFPGRFEDLETGHFEPAAAAAGRRRRSGLEALAGRHLKFMADFGRRLEEEEK
                     "
     gene            727..1293
                     /locus_tag="PKN80_06580"
     CDS             727..1293
                     /locus_tag="PKN80_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNQ35715.1"
                     /translation="MKKVFAAILVAVGLLLLIFLVIQAQYNRLVSLDQKVAESWSQVQ
                     NVLQRRADLIPNLVSTVKGYARHEKELFIQVTEARSRLMSAGGVAETSAADRELSSAL
                     SRLLVVVENYPQLKANENFLRLQDELAGTENRISVERRNYNLAVRDYNRTVKSIPIVF
                     FIRMLPFDAEKEYFEAAPGAESVPKVEF"
     gene            complement(1368..2519)
                     /locus_tag="PKN80_06585"
     CDS             complement(1368..2519)
                     /locus_tag="PKN80_06585"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNQ35716.1"
                     /translation="MNGISEIARLKTGRRTKRISSYDVSGRNADYWKIPAGQARVLAD
                     IKGPACINHLWMTQANHYRECLIRITFEESRNPSILVPLGDFFCLGHGIVNSFQSLYF
                     TASTRNNNRFNIGCALNCYLPMFFRKRAVVELVNESEEEHLQYFYVDYEELEEVPETA
                     GYLHAEFRRTNPFGGWGHEVRVNSPEADIVNKERMAWENNYLILETKGRGHFIGCNLS
                     VSNFQGTWWGEGDDMIWVDGYKWPPDLHGTGSEDFLNQAWGMQENAFLRNGSSIHELK
                     TGGYQTSYIFYPENPVRFEKEIRVSIEHGHGNHLANEMSSVAYWYAEKPAAAARPPAV
                     ARRLPVGREQFTNRWVQRPGGTITPFVVKLNGEMKRMKEAWRKKMSPKK"
     gene            complement(2771..4258)
                     /locus_tag="PKN80_06590"
     CDS             complement(2771..4258)
                     /locus_tag="PKN80_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35717.1"
                     /translation="MAEVPLYEISRDPAGSPPRRFFFFDEELPEAAYGVRRVYGPVTR
                     KPARLAGNPAGVNTSAFAGSIVKLAGGGYRLYATSYLSGERTMEITAWESERGLDWKP
                     VKLPGGQASRLGKNILRIRNLEDRLLSYQPQVFQLGPKNWVMYSWNRFQNNHLSYLRA
                     VSTDGLDWRADTATEPVLYHPSRFGLGEWVRGLPLREIKELAISESEALRCLQRASND
                     SVYIYHHPESGGYECYSVWLHPAIPDRRVEVDNASSVLRLIQRRWSRDGRRWSDPELV
                     VMPDGRDPWDLQFYYLAVQWHRNWRIGSLGRYRVEDGQQTMDLELCFSRDGRKWERPL
                     RAGFIPRPPENSGEIDTLMVYGPNTWLDDGKDWLCLYTGHPTRHNVRVEKRTGLIGAR
                     FRKDRLAGLAADRTGGGFLTEPFILAGPELKVDAAVRGWIRAELCDAFGRKLPGFHLM
                     DCRPLTGDREAHRLAWKGGRLSDHLYRALRLRFELQDAEIYNFYF"
     gene            complement(4290..>4754)
                     /locus_tag="PKN80_06595"
     CDS             complement(4290..>4754)
                     /locus_tag="PKN80_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNQ35718.1"
                     /translation="LPLDRHRSLGNNAQLLELAGARITGEFGEYEGQTISWRGEFPQA
                     ADRKLLATRLAKALKTSCRLLSFGPERIKSIAVCSGGGGYGLFEAALAAGVDLYLTGE
                     SIEVYHAARDAGLNVIFAGHHATETLGPRALAREVGRKFKLPVSFLDLPTGL"
BASE COUNT          783 a         1569 c         1479 g          923 t
ORIGIN      
        1 cggtttcggc ggcggttcct cgggcggcgg cggagccagc ggcggctggt gacggcggat
       61 caaaggagaa aacatggctg acctggaaag agttctggaa accttcaaat cgaaactgct
      121 cgaaatctgg ggggacgatc tgcagagcct gctcctctac ggcagctacc tggggccgga
      181 cttccagccc ggccattccg acctcaacct gatggcggtg gtgcgccgcg acgatccgga
      241 gaaactcttc cgctcgaggc ggctggtcgc ctcctttcac cggcgccgcc ggctggtgcc
      301 gctcttcttt acggccgaat ttctcaagga gtccctggat actttcccgg tcgagtttga
      361 ggccatccgg cgccgttacc gggtgctcca cggctccgac ccggtggccg gcctggacct
      421 gtcgaaggcc gacctccgct tgcagctgga acgggaaata aaggagaata tcatcaggtt
      481 ccgccagggg atcctctttg gctgggagat cccgtccctg ctggggagct cggtgaaatc
      541 aatgcgcatc ctgctggagg cggcccggct cgcctttccc ggccgtttcg aagacctgga
      601 gaccggccac ttcgagccgg ccgcggcggc ggccggacgc cggcgccgct ccggcctcga
      661 agccctggcc ggccgccacc tgaaattcat ggccgatttc ggccgacgcc tggaagagga
      721 ggagaaatga agaaggtatt tgctgccata ctcgtggccg tcgggttact gctgctgatt
      781 ttcctggtca tccaggccca gtacaaccgc ctggtcagcc tcgaccagaa ggtggccgag
      841 agctggtccc aggtgcagaa cgtcctccag cggcgggccg acctcattcc caacctggtc
      901 tcgaccgtca agggatacgc ccgccacgag aaggaactct tcatccaggt caccgaggcc
      961 cggagccgcc tcatgtccgc cggcggcgtg gccgagacct cggccgccga ccgggagctc
     1021 tccagcgcgc tcagccgcct cctggtggtg gtcgagaact acccgcagct caaggccaac
     1081 gagaacttcc tgcgcctcca ggacgagctg gccggcaccg aaaaccgcat ctcggttgag
     1141 cggcgcaatt acaacctggc ggtccgcgac tacaaccgca ccgtcaagag catcccgatc
     1201 gtcttcttca tccggatgct tcccttcgac gccgaaaagg agtacttcga ggccgcgccc
     1261 ggcgccgaga gcgtgccgaa agtcgaattc tgagcttaag ctgatatcaa taaaaaaagg
     1321 gggacgcctt ccgggcgccc cccttctttt ttgccgggtc agccggccta ctttttcgga
     1381 gacatcttct tccgccaggc ctctttcatc cgcttcatct cgccgttcag cttgaccacg
     1441 aagggcgtga ttgtgccgcc cggccgctgg acccagcggt tggtgaactg ctcccggccg
     1501 accggcaggc gcctggccac cgccggcggc ctggccgccg cggccggttt ctccgcgtac
     1561 cagtaggcca cgctgctcat ctcgttggcc aggtggttgc cgtggccgtg ctcgatgctc
     1621 acccggatct ccttctcgaa gcggaccggg ttctcgggat agaagatgta gctggtctgg
     1681 tagccgccgg tcttcagctc gtggatcgag gagccgttgc gcaggaaggc attctcctgc
     1741 atgccccagg cctggttcag gaagtcctcg ctcccggtcc cgtgcaggtc gggcggccac
     1801 ttgtagccgt ccacccagat catgtcgtcg ccctcgcccc accaggtccc ctggaagttg
     1861 ctcacgctca ggttgcagcc gatgaagtgg ccgcgcccct tcgtctccag gatcaggtaa
     1921 ttattctccc aggccatccg ctccttgttg acgatgtcgg cctccgggct gttcacccgg
     1981 acctcgtggc cccagccgcc gaaggggttg gtccgccgga actcggcatg caggtagccg
     2041 gccgtctccg ggacctcctc cagctcctcg taatcgacgt agaagtactg caggtgctcc
     2101 tcttcgctct cgtttaccag ctccaccacc gcccgcttcc ggaagaacat cggcaggtag
     2161 cagttcaggg cgcagccgat gttgaagcgg ttgttgttgc gggtcgaggc ggtgaagtaa
     2221 agcgactgga aggagttgac gatcccatgg cccaggcaga agaagtcgcc cagcgggacc
     2281 aggatgctgg ggttcctgga ctcctcgaag gtgatccgga tcaggcactc ccggtaatgg
     2341 ttggcctggg tcatccagag atggttgatg caggccggtc ccttgatatc ggccaggacc
     2401 cgcgcctggc cggccggaat tttccagtaa tccgcgttcc ggccgctcac gtcgtaggat
     2461 gaaatccgct tcgttcttcg cccggttttc aaccgggcaa tctcactgat tccgttcata
     2521 ttctctccct gtcccctcat gtagtaggag ggaatccagc ctttattttt cttaaatgct
     2581 tttgatagtt taccaggtag cgtggatacc cgtctaaaac ttacgggtat gacgggcttt
     2641 tgagtgtctt gtttcattac ttttcgtctg tcatttccgc ggcttgttgg cggaaatcca
     2701 gggctacctg taataccctc aactgctttt aaagtggata cccgttaact atccacgggt
     2761 atgccgtgcc tcaaaaataa aaattataaa tctccgcgtc ctgcaattca aagcgcaggc
     2821 gcagggcccg gtaaagatgg tcgctcagcc ggccgccctt ccaggccagc cggtgcgcct
     2881 cccggtcccc ggtcagcggc cggcagtcca tcaggtggaa gccgggcagc ttccggccga
     2941 aggcgtcgca caactcggcc ctgatccagc cgcgcaccgc cgcgtccacc ttcagctccg
     3001 gcccggccag gatgaagggc tcagtcagga agccgccgcc cgtccggtcg gcggccagtc
     3061 cggccagccg gtccttccgg aagcgggcgc cgatcaggcc ggtgcgtttc tcgacccgca
     3121 cgttgtggcg ggtcggatgg ccggtgtaga ggcagagcca atcctttccg tcatccagcc
     3181 aggtattcgg cccgtagacc attagcgtgt ctatctcacc gctgttctcc ggcggccggg
     3241 gtatgaagcc ggcccgcagc ggccgctccc atttccggcc gtcgcgcgag aagcagagtt
     3301 ccaggtccat cgtctgctgg ccgtcctcca cccggtagcg gcccaggctg ccgatccgcc
     3361 agttccggtg ccactggacc gccaggtagt agaactgcag gtcccaggga tcgcggccgt
     3421 cgggcataac gaccagctcc ggatcgctcc agcggcggcc gtcccggctc cagcgccgct
     3481 ggatgagccg cagcaccgac gaggcattgt caacctccac ccgccggtcc ggtatcgccg
     3541 ggtggagcca gaccgagtag cattcgtagc cgccgctttc cgggtggtga tagatataga
     3601 ccgaatcgtt gctggcccgc tgcaggcatc gcagagcctc gctttcactt atggccagct
     3661 ccttgatttc gcgcagcggc aggccccgga cccattcgcc gagtccgaac ctggacggat
     3721 ggtagaggac cggttccgtt gccgtgtccg cccgccagtc caggccgtcg gtgctcaccg
     3781 cccgcaggta ggagagatgg ttgttctgga agcggttcca ggagtacatc acccagttct
     3841 tcggtcccag ctggaagacc tggggctggt aggagagcag ccggtcctcc aggttcctga
     3901 ttcggagaat gttctttccc aggcggcttg cctggccgcc cggcagtttc accggcttcc
     3961 aatccaggcc ccgctccgac tcccaggccg ttatctccat ggtccgttcg cccgaaaggt
     4021 agctggtggc gtagagccgg tagccgccgc cggccagctt cacgatgctg ccggcgaagg
     4081 cggaggtatt caccccggcc gggttgccgg ccaggcgggc cggtttcctg gtcaccggtc
     4141 cgtaaacgcg gcgcacgccg taggcggcct ccggcagctc ttcatcgaag aagaagaaac
     4201 gccgcggcgg acttccggcc gggtcgcgtg aaatctcgta gagcggtacc tctgccattt
     4261 ttttactcca ctttcgggtt ggaaagtgat cagagcccgg tcggcaggtc caggaaggag
     4321 accggcagct tgaacttccg gccgacctcc cgggccaggg ccctggggcc gagcgtttcg
     4381 gtggcgtggt ggccggcgaa gatgacgttt aggccggcgt cgcgggcggc gtggtaaacc
     4441 tctatcgatt ccccggtcag gtagagatcg accccggccg ccagggccgc ctcaaagagg
     4501 ccgtagccgc cgcctccgct gcagacggcg atgctcttga tccgctccgg accgaaggag
     4561 agcagccggc agctggtctt cagcgccttc gcgagccggg tcgccaggag cttccggtcg
     4621 gcggcctggg gaaactcgcc ccgccagctg atcgtctgac cctcgtactc tccgaactcc
     4681 ccggttatcc gggcgccggc cagttcgagc agctgggcgt tgttcccgag gctccggtgc
     4741 cggtccaggg gcag
//
LOCUS       DAOQYN010000153         4732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_18359_length_4732_cov_4.428907, whole genome shotgun sequence.
ACCESSION   DAOQYN010000153 DAOQYN010000000
VERSION     DAOQYN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18359_length_4732_cov_4.428907"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PKN80_06600"
     CDS             <1..1416
                     /locus_tag="PKN80_06600"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HNQ35719.1"
                     /translation="FCSENRNGLVVESVGPRPEHPEYWRKLLELVRVYHAPGRFLTLP
                     GYEWHGDRARYGDHNVIYFESGGRLDAAWRLEDLYRNLAGGRALVIPHHTGYAPAYRG
                     KDWSVHDDSLSPVMEIYSIHGSSEGCETPFPMERNTNLGPRVSAGTFQAGLARGCRLG
                     VIASNDGPGLPGRWGRGRAAAWVKEFSREGLLEAFRARRTYAVTGDRIELRFRVNGAE
                     MGASVPGGAAVEAEAEVIGAAALDRLELVQNGRVAATYNHSGRWETKAARERRFKVAL
                     EVGWGPHAKYGFKLTEKFWRWQGSLAVAGGRLAGLENRFTLPGQRAVLAAPGRSHFEL
                     ASRVKYDSSPTNIQQGLVFEIEGGAGTGLDFDIEGLRFRAEIGELLAGTRFVPLIRES
                     EARIRKEFGLDGESIGNPDPYILNCRKIKLHRAVPESGYRVGRRFRNLRLDKGLNYFY
                     LRASQLNGQYAWSSPVWVTRA"
     gene            1436..2503
                     /locus_tag="PKN80_06605"
     CDS             1436..2503
                     /locus_tag="PKN80_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35720.1"
                     /translation="MKKLTSHERFARIFGHREADRIPIVDAPWSTTIQRWRREGMPEN
                     VDFTEFFDLDRVAGFSIDNSPRYPVTVLEETDEYTIATSVWGVTVKRWKQATSTPQFL
                     DFRVVSPATWAEARRLIEPAEDRIPWERLKENYPRWKAAGLWISAGLWFGFDITHSHM
                     VGTERLLVAMIEEPEWCRDMFNFLLDVQLVMLEKVWAAGYRFDEVHWYDDMGYKHNQF
                     FSVRTYRDLLKPAHKKAIDWAHGHDVRAHLHSCGDIRPFIPELAGIGLDALNPLEVKA
                     GVDPLALKREFGSRMTFKGGINATLWDKPDLIEAEIRRLVPRMKESGGYIFASDHSVP
                     DAVSLEDFRRITDLAKELGRY"
     gene            2675..3253
                     /locus_tag="PKN80_06610"
     CDS             2675..3253
                     /locus_tag="PKN80_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNQ35721.1"
                     /translation="MGKSVKGTQTEKNLLAAFAGESQARNRYTYFASVAAKAGYVQIK
                     NIFLETAENEKEHAKLFFKALEGGEVTITAGYPAGTIEDTRANLQAAADGEKMEWSTI
                     YADFAKAARDEGFPEIARTFEQVAKVEKFHESRYRKLAANLDADQVFKKPAPVKWHCT
                     NCGYVFEGGEAPRECPACRHPQAYFELLAENY"
     gene            complement(3250..>4732)
                     /locus_tag="PKN80_06615"
     CDS             complement(3250..>4732)
                     /locus_tag="PKN80_06615"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HNQ35722.1"
                     /translation="WCPRCLELFRKETGVARPPRSKEEPHWREWLAFHRRAFENHVRL
                     YTEAVHRRKPGCLVCSNWLYSVRQPEEVSLPVDYLSGDFTPSFGCERAAIEARFLASR
                     GLTWDLMAWGFYGPAEKYPWEFKTAEHLQQEAAEVIANGGGLFVYDVPIRSGRLTGWH
                     QDILAEVARFCRDRQPYCQGTESLPQAALLHNSLDYYNNNDPLYKLARATEHVEGALQ
                     SLLENHCHVDILNEKALAERIEEYPLVVLPEVEELPEEFLAACGRYVRKGGHLLVSGE
                     RSAARLGKVLGVEPEGEASERTAGLPVERQVVMATGRWQPVKTAGGRVLAYEQELDGR
                     RVEPARPLATIHRLGRGKAVGIYGPVFRNFFNTRSPRLRAFLGRVLAEMDNPHLIRVS
                     APPRIEVILRRDEKRILVHLLNRSAGHPLSNRNHAVEEVPPAGPVGLSLPLAERPKRV
                     RLAPGRGRCGARWRNGRLEVTVAAVGVHQVVVVELGRKKKGRT"
BASE COUNT          793 a         1585 c         1574 g          780 t
ORIGIN      
        1 ttctgcagcg agaaccgcaa cgggctggtg gtggagagcg tcggtccccg gccggagcac
       61 ccggaatact ggcgaaaact gctggaactg gtccgggtct atcacgcccc gggccgcttc
      121 ctgaccctgc ccggctacga gtggcacggc gaccgcgccc gttacggcga ccacaacgtc
      181 atctactttg aatccggcgg ccggctggac gcggcctggc ggctcgagga cctctaccgg
      241 aacctggccg gcggccgggc cctggtcatt ccccaccaca ccggctacgc gcccgcctac
      301 cggggcaagg actggagtgt ccacgatgat tcgctctccc cggttatgga gatctactcg
      361 atccacggct cctcggaggg gtgcgaaacg cccttcccga tggagcgcaa caccaacctc
      421 ggaccgcgcg tttcggccgg caccttccag gccggtctgg cccggggctg tcggctcgga
      481 gtgatcgcct ccaacgacgg gcccggcctg ccgggtcgct gggggcgggg ccgggcggcc
      541 gcctgggtga aggagttcag ccgggaaggc ctgctggagg ccttccgggc gcgccggacc
      601 tacgcggtca ccggcgaccg gatcgaactg cggttccggg tcaacggcgc cgaaatgggc
      661 gcctcggttc ccggcggcgc cgcggtcgag gccgaggctg aggtgatcgg cgccgccgcc
      721 ttggaccggc tggagctggt ccagaacggc cgggtggccg ccacttacaa ccacagcggc
      781 cgctgggaga cgaaggcggc gcgcgaaagg cgcttcaagg tggctctcga agtcggctgg
      841 gggccgcacg ccaagtacgg cttcaaactt accgaaaaat tctggcgctg gcagggcagc
      901 ctggcggtgg ccggcggccg cctggccggc ctggagaatc gctttaccct gcccgggcag
      961 cgggcggtgc tggccgcacc cggccgcagc cacttcgaac tggccagccg ggtcaagtac
     1021 gattccagcc cgaccaacat ccagcagggc ctggtattcg aaatcgaggg cggggccggg
     1081 acgggactgg atttcgatat cgaggggctg cgctttcggg ccgaaattgg cgaactgctg
     1141 gccgggactc ggttcgtccc actcatccgg gaatcggagg cgcgcatccg gaaggaattc
     1201 ggcctggacg gagagagcat cggcaatccc gatccataca tcctcaactg ccgcaagatc
     1261 aagctccacc gggcggtgcc ggagtccggc taccgggtcg gacgccggtt ccggaacctg
     1321 cgcctggata agggtcttaa ctacttctac ctgagggcct cccagctgaa cggccagtat
     1381 gcctggtcgt cgccggtctg ggtgacccgg gcctgacctc caccaaggag tccgaatgaa
     1441 gaaactgact tcgcacgaac gcttcgcccg gatcttcggc caccgggagg ccgaccggat
     1501 cccgatcgtc gatgccccct ggtcgaccac catccagcgc tggcggcgcg aaggcatgcc
     1561 ggaaaacgta gatttcaccg aatttttcga cctcgaccgg gtggccggct tttcgatcga
     1621 caacagcccc cgctacccgg tcacggtgct cgaagagacc gatgagtata ccatcgccac
     1681 ctcggtctgg ggggtgacgg tcaagcgctg gaaacaggcc acttccacac cccagttcct
     1741 cgacttccgg gtcgtttcgc ccgccacctg ggccgaggcg cgccgactca tcgagccggc
     1801 cgaagaccgc atcccctggg agcgcctcaa ggaaaattac ccgcgctgga aggccgccgg
     1861 actctggatc tcggccggcc tctggtttgg tttcgatatc acccactcgc acatggtcgg
     1921 caccgagcgg ctgctggtgg ccatgatcga ggaaccggag tggtgccggg acatgttcaa
     1981 ttttctgctc gacgtccagc tggtgatgct ggagaaggtt tgggcggccg gctaccggtt
     2041 cgacgaggtt cactggtacg acgacatggg ctacaagcac aaccagttct tctcggtccg
     2101 gacctaccgc gacctgctca agccggcgca caagaaggcg atcgactggg cccatggcca
     2161 cgacgtccgg gcccatctcc actcctgcgg ggatatccgc cccttcattc cggaactggc
     2221 cgggatcggc ttggacgccc tcaacccgct ggaggtcaag gccggcgtgg acccgctggc
     2281 cctgaaacgg gaattcggct cgcggatgac cttcaagggc gggatcaatg ccacgctctg
     2341 ggacaagccg gacctgatcg aggccgagat ccggcggctg gtgccccgga tgaaggaatc
     2401 gggcggctac atctttgcct ccgaccactc ggtccccgac gccgtcagcc tggaggattt
     2461 ccggcggatt acggatctgg ccaaggagct gggccgctac tgatcccggc ctgtcttcgg
     2521 cccggccagc cttgttggcc gcgtcggcgc cgtcgtcggc gtacctccgg taggcctccc
     2581 tggtttctcc ttgccggggc gggtgccggt cagttataat atttaatact aactcgtttt
     2641 aaatcctgga attttattaa acaaggaggg gagcatgggc aagtcagtca agggcacgca
     2701 gaccgagaaa aacctgctgg ccgccttcgc cggcgagtcg caggcccgca accgctacac
     2761 ctacttcgcc agcgtggccg ccaaggccgg ttacgtgcag atcaagaaca tcttcctgga
     2821 gacggccgag aacgagaagg agcatgccaa gctcttcttc aaggccctgg aaggcggcga
     2881 ggttaccatc accgccggct acccggccgg gacgatcgag gacacccggg ccaacctgca
     2941 ggcggccgcc gacggcgaaa agatggaatg gagtactatc tacgccgact ttgccaaagc
     3001 cgcccgcgac gaggggttcc cggagatcgc ccggaccttc gagcaggtgg ccaaggttga
     3061 gaagttccac gagtcccgct accggaaact ggccgccaac cttgatgcgg accaagtctt
     3121 caagaagccg gcgccggtga agtggcactg caccaactgc ggttacgtct tcgaaggcgg
     3181 cgaggcgccc agggagtgcc cggcctgccg ccatccgcag gcctattttg aattgctggc
     3241 cgaaaattat taagtccggc ctttcttctt ccggcccagt tccaccacca cgacctggtg
     3301 gacgccgacg gcggccaccg ttacctccag gcgtccgttc cgccagcggg cgccgcagcg
     3361 tccacggccg ggcgccaggc ggacccgctt cggccgttcc gccagcggaa gactgagtcc
     3421 gaccgggccg gccggcggca cctcttcgac cgcgtggttc cggttggaaa gcgggtggcc
     3481 ggcgctccgg ttcagcaggt gcaccaggat ccgcttctca tcccggcgca ggatcacctc
     3541 gatccgcggc ggggccgaca ccctgatcag gtgcgggttg tccatttcgg ccagcacccg
     3601 gccgaggaag gcgcgcaggc ggggggaacg ggtgttgaag aagttgcgga agaccggccc
     3661 gtagatgccg accgctttgc cccggccgag acggtggatc gtcgccagcg gccgggccgg
     3721 ctcgacccgc cgtccgtcca gttcctgctc ataagcgaga acgcggccgc cggcggtctt
     3781 aaccggctgc cagcggccgg tcgccatgac cacctgccgc tcgaccggca ggccggcggt
     3841 ccgttccgac gcctctccct ccggttcgac gcccagcacc tttcccagcc gggccgccga
     3901 ccgttcgccg cttaccagca ggtggccgcc cttccggaca taacggccgc aggcggccag
     3961 gaattcctcc ggcagctctt ccacctccgg cagcaccacc agcgggtact cttctatccg
     4021 ttccgccagg gccttctcgt tgagaatgtc cacgtggcag tggttttcca gcagcgactg
     4081 cagggcgccc tccacgtgct cggtggcccg ggccagcttg taaagcgggt cgttgttgtt
     4141 gtagtagtcg agagagttgt ggagcagggc ggcctggggg agcgattcgg ttccctggca
     4201 gtagggctgg cggtcccggc agaagcgggc cacctcggcc aggatgtcct ggtgccatcc
     4261 ggtcagccgg ccggagcgga tcggcacgtc gtaaacgaag aggccgccgc cgttggcgat
     4321 cacctcggcc gcctcctgct ggaggtgttc ggccgtcttg aattcccagg ggtacttttc
     4381 ggccgggccg tagaagcccc aggccatcag gtcccaggtc aggccccggc ttgccaggaa
     4441 gcgggcctcg atggccgccc gctcgcagcc gaaggagggg gtgaagtcgc cgctcaggta
     4501 atccaccggc aggctgacct cctccggctg gcggaccgag tagagccagt tgctgcagac
     4561 caggcagccg ggtttgcggc ggtggaccgc ctcggtgtag aggcgcacgt gattttcgaa
     4621 ggcgcggcgg tgaaaggcca gccactccct ccagtgcggc tcctcctttg accggggcgg
     4681 ccgggcgacc ccggtctcct tcctgaagag ttccaggcag cgcgggcacc ag
//
LOCUS       DAOQYN010000154         4702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_18504_length_4702_cov_4.430816, whole genome shotgun sequence.
ACCESSION   DAOQYN010000154 DAOQYN010000000
VERSION     DAOQYN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18504_length_4702_cov_4.430816"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKN80_06620"
     CDS             complement(<1..266)
                     /locus_tag="PKN80_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35723.1"
                     /translation="MKKAVASGSYEVNGKRLERFSRRESGRAILFRTVQPEGIEITVT
                     VRRRRGRSGGLEISHRIANRSKRELVLGACHHFSIEFSSSEPAEV"
     gene            725..1360
                     /locus_tag="PKN80_06625"
     CDS             725..1360
                     /locus_tag="PKN80_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNQ35724.1"
                     /translation="MKKRGFTLIELLVVIGIIAILAAMLLPALARARERARVVNCINN
                     LKQIGLAARMYAEDFNGIMPGESGGNTNNFFVKMVNAGYIKSQNVGKCPSEKTYANVN
                     IPHYGINYRSLGGSSADPRINFTDRPDRLAYLTDRNSSGTNFLVGAASDEAAFGCNWW
                     RHSFNPTGPKDAAGINEDCRMNFLFLDGHVENLSRNDAPNNQNVYPWTATY"
     gene            1404..2195
                     /locus_tag="PKN80_06630"
     CDS             1404..2195
                     /locus_tag="PKN80_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ35725.1"
                     /translation="MKRKGFTLIELLVVIAIIAILAAMLLPALARARERSLIASCQNN
                     LKQLHLALELYANDYDGWYPHDKELSLLLPGADTQFAATAKGSLALLISGNYVKNTKV
                     FLCPGDNVNQVPAASYSDFVAVGNKNIRCSYAFAVGYRNTDFVSTKVEQPERIVLLLD
                     RGQQELYTDPYENQPGSESRCATYHNGLRYYARGGARNNHGYVGGNFLYALGNVEFKP
                     CDSFSGTGTGRYGTYANYWPAVNAYNNAGWRNGGRLKKWYGPRAW"
     gene            2505..3281
                     /locus_tag="PKN80_06635"
     CDS             2505..3281
                     /locus_tag="PKN80_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ35726.1"
                     /translation="MKKRGFTLIELLVVIAIIAILAALLLPALANAREKARQANCISN
                     LKNLYTCLYMYAQDFDEWFPYDGLTDANGQPVYTFDSMDTWHPRAWATCCLRLLWVNG
                     YVKDAGIFICPSGSYKKAAMSSAGDLKAANIAYAYTCGVKLRDRYPNELVLMCDKHYS
                     YTAPAAGSGPDDIPYPTSAQRTSGIYWNGDYTYKAQAQCNHWMGMNVMYWKGNVEWLP
                     VKVVSGNQGYFLGVVGGNQKAVINGFRGDTTNKYPAYMAF"
     gene            3299..3940
                     /locus_tag="PKN80_06640"
     CDS             3299..3940
                     /locus_tag="PKN80_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ35727.1"
                     /translation="MGRKKGFTLIELLVVIAIIGILAAMLLPALSRARGRAQQATCMA
                     NLKQLGLAFHQYINDWDGYPPCNQGAFGGGGMNGWLGQLSPYLGINQDGTATDIWNRM
                     KVHKLVICPSPRTAYRIYGINYRGWAGGLVTTNKLENLGLSLDKFIYVGEVRTSSTYS
                     LSNSERPVDYERHYGQANFLFADGHVESGNTDQFGPYYISTAGSPFAVYWTGR"
     gene            complement(4028..>4702)
                     /locus_tag="PKN80_06645"
     CDS             complement(4028..>4702)
                     /locus_tag="PKN80_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35728.1"
                     /translation="QEQREHESGGRLLITEKSGRFTDEPRRRARPAFDRLFSGSNLRT
                     AGSRQEETGLIESHRQFTEREFGGERARAEYGRGRAAYLPRLDYLYRPRAFQSRYHVF
                     YDGIDSRYWKEPLNRDEILAELEWLYPDCRPLRVAEAPELRLDYLAWPDGRLGAALLR
                     CGTLAGPADLRLGVRAAAAPLDGELWRPGDERPLKLAWRREKDYFETGLAGVGRHGVV
                     RWRLKE"
BASE COUNT          924 a         1470 c         1439 g          869 t
ORIGIN      
        1 acttcggccg gttcggaact ggaaaattcg attgagaagt ggtgacaggc gcccagtacg
       61 agttcccgct tcgagcggtt ggcgattcgg tgggatatct ccaggccgcc gctccttccc
      121 cggcgccggc ggacggtcac cgttatttca atcccctccg gctggacggt ccggaaaagg
      181 atcgcccggc cgctctcccg ccgggagaaa cgctccagcc gctttccgtt gacttcgtat
      241 gaaccgctcg ctaccgcttt tttcatgcca gtccgccggt ttggtcgggc cgatggagcc
      301 gggccggctc atggtttaag agggaaccgc gggagggaag ttgcgctttc ctgtccttat
      361 atattactat actaatggcc gattgaacaa atgcctcttc cggaaaccgg ccggcgtctt
      421 ccccggcgga agatccgcgg ttcgaccggc cggggcgtga cggcgggaac cgccataaca
      481 cttaaacaaa catattgaga aaacaggcaa tagaaagagc gtgagaattt gagacggcaa
      541 ccttttcagc cgctacttga caaatgggtc ccggtgtgaa aaaatagagt agatttaagg
      601 cggataacgc ctccggaatt tcgggggttt gattattgag aaaggaggtg atggagagag
      661 atcgacggcc gattctgctc tttttgagtt cggataacat cattatcatc attggaggca
      721 gtccatgaag aaacgaggtt tcaccctgat tgaattgctg gtggtcatag ggatcatcgc
      781 tatattggcg gcgatgctgc tgccggccct ggcccgggcc cgggaacggg cgcgggtggt
      841 caattgcatt aataacctga agcagatcgg gctggcggcc cggatgtacg ccgaggactt
      901 caacggcatc atgcccggcg agtccggcgg caacaccaac aacttcttcg tgaagatggt
      961 caatgccggt tacatcaaga gccagaatgt cggcaagtgc ccgtccgaga agacctacgc
     1021 caacgtcaac atcccccact acgggattaa ctaccgttcc ttggggggca gctcggccga
     1081 cccgcggatc aacttcaccg accggccgga ccggctggcc tacctgaccg accggaactc
     1141 cagcggcacc aacttcctgg tgggcgcggc ctccgacgag gcggccttcg ggtgcaactg
     1201 gtggcgccac agcttcaacc cgaccggtcc caaggacgcg gccggcatca acgaggactg
     1261 caggatgaac ttcctcttcc tggacggcca cgttgagaac ctgtcgcgca atgacgcccc
     1321 gaataaccag aacgtttatc cctggacggc cacttattaa gccggatgaa gcacactgat
     1381 cattctatca ggagagatta aagatgaaac gaaaaggatt cacgctcata gaattgctgg
     1441 tggtgatcgc catcatcgcg attctggcgg cgatgctgct gccggccctg gcccgggccc
     1501 gggaacggtc gctgatcgcc tcctgccaga acaacctgaa gcagcttcac ctggccctgg
     1561 aactctacgc caacgactac gatggctggt atccccacga caaggagttg agcctgttgc
     1621 tgccgggcgc ggacacccag ttcgcggcca cggccaaggg ctccctggcg ctgctcattt
     1681 cgggcaacta cgtcaagaac accaaggtct tcctctgccc gggcgacaac gtcaaccagg
     1741 taccggcggc cagttacagc gattttgtcg ccgtcggcaa caagaatatc cgttgcagtt
     1801 acgccttcgc agtcggctac cggaacaccg attttgtctc caccaaggtc gaacagccgg
     1861 agcgcatcgt cctcctgctg gatcgggggc aacaggagtt atacaccgat ccttacgaga
     1921 accagccggg atccgaaagc cgctgcgcca cttaccacaa cgggcttcgc tactacgccc
     1981 ggggcggcgc ccgaaataac cacggctacg tgggcggaaa ctttctttac gccttaggca
     2041 acgtggaatt caagccctgc gactccttct ccggcaccgg caccggccgg tatggaacct
     2101 acgccaacta ctggccggcg gttaacgcct ataacaacgc cggctggcgc aacggcggcc
     2161 ggctcaaaaa gtggtacgga ccgcgggctt ggtaagggat agttaagcaa gctaaatcgg
     2221 gatatcggcg ccgggaggcc tcttttcaac ctcccggcgc tttttttctc cccctcccgt
     2281 cccgccactt ccttcggccg cttttctgga aagggggcgc ccgccccggt cgccccttta
     2341 catttcggat tggtaagggt ataagaaaag tatcattaac aatctcgagt ttttaacttg
     2401 acaatacgca tattgattgt cgaaaggggg tgagcggccg ggcggagaaa ttttcggaac
     2461 ggtgaacgga tcgtttcagt atcgaggtca tccggaggtc tgcgatgaag aagcgaggtt
     2521 ttaccctgat cgaactgctg gtggtgatcg ccatcatcgc catcctggcg gcgctcctgc
     2581 tgccggccct ggccaacgcg cgggagaaag cccggcaggc caattgcatc agcaacctga
     2641 agaacctcta cacctgcctt tacatgtatg cccaggattt cgacgagtgg ttcccctacg
     2701 acggcctgac cgacgccaac ggccagcccg tctacacctt cgacagcatg gatacctggc
     2761 acccgcgggc ctgggcgacc tgctgcctgc gcctgctctg ggtgaacggc tacgtcaagg
     2821 acgccggcat cttcatctgt ccgtccggct cctacaagaa ggcggccatg agcagcgccg
     2881 gcgaccttaa ggcggccaac atcgcctacg cctacacctg cggggtcaag ctgcgcgacc
     2941 gctacccgaa cgaactggtg ctgatgtgcg acaagcatta cagctacaca gcgccggccg
     3001 ccggctccgg cccggacgac atcccctacc cgacctcggc ccagcggacc tccggcatct
     3061 actggaacgg tgattacacc tacaaggcgc aggcccagtg caaccactgg atgggcatga
     3121 acgtgatgta ctggaagggt aacgtggagt ggctgccggt caaggtggtc tccggcaacc
     3181 agggctactt cctgggcgtg gtcggcggca accagaaggc ggttatcaac gggttccggg
     3241 gcgacaccac caacaagtac ccggcctaca tggccttttg agcgggaggg attcgacgat
     3301 gggacgaaag aaagggttta ccctgataga actgctggtg gtgattgcga tcatcggcat
     3361 cctggcggcg atgctgctgc cggccctctc ccgggcccgg ggccgggccc agcaggccac
     3421 ctgcatggcc aacctgaagc agctgggcct ggccttccac cagtacatca acgactggga
     3481 cggctacccg ccctgcaacc agggggcttt cggcggcggc ggcatgaacg gctggctggg
     3541 gcagctctcg ccctacctgg gcatcaacca ggacggcacc gccaccgata tctggaaccg
     3601 gatgaaggtt cacaaactgg tgatttgccc ctccccccgg acagcttatc ggatttacgg
     3661 catcaactac cggggctggg cgggcggcct ggtgacgacc aacaagttgg agaacttggg
     3721 cctgagcctg gacaagttca tctacgtggg ggaggtcagg acctccagca cctattcact
     3781 cagcaactcc gagcggccgg tcgattatga acgccactac ggccaggcca acttcctctt
     3841 cgccgacggc catgtcgaga gcggcaacac cgatcagttc ggaccctact acatcagcac
     3901 ggccggctcg cccttcgcgg tttactggac cggccggtaa cggcggcggt cggccgggat
     3961 aaaaggagga gccgggcctt cagcggtttg aagacccggc tcctcctttt ttttatccgg
     4021 ttgccggcta ctctttcagc cgccagcgga ccaccccgtg gcggccgact ccggccaggc
     4081 cggtctcgaa gtaatccttc tcccgccgcc acgccagttt gaggggcctt tcatcgcccg
     4141 ggcgccataa ttcgccatcc agcggcgccg cggcggcccg gacgcccagg cgcaggtcgg
     4201 ccgggccggc cagcgttccg cagcgcagca gggcggcgcc cagccggccg tccggccagg
     4261 ccaggtaatc gaggcgcagt tccggggcct cggccacccg gagcggccgg cagtccgggt
     4321 agagccactc gagttcggcc aggatttcgt cccggttgag cggctccttc cagtagcggc
     4381 tgtcgatgcc gtcgtagaag acgtggtagc ggctctggaa ggcgcggggc cggtagaggt
     4441 agtcgagccg gggcaggtag gcggcccggc cgcggccgta ctcggcccgg gctctttcgc
     4501 ccccgaactc ccgctcggtg aactggcggt gtgactcgat gaggccggtc tcctcctggc
     4561 ggctgcccgc ggtccggagg tttgagccgc tgaagaggcg gtcgaaggcc ggccgggccc
     4621 gccggcgcgg ctcgtcggtg aaacggccgg acttctcggt gatcaggagg cggccgccgg
     4681 actcgtgctc gcgctgttcc tg
//
LOCUS       DAOQYN010000155         4692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_18560_length_4692_cov_4.432607, whole genome shotgun sequence.
ACCESSION   DAOQYN010000155 DAOQYN010000000
VERSION     DAOQYN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18560_length_4692_cov_4.432607"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1797
                     /locus_tag="PKN80_06650"
     CDS             <1..1797
                     /locus_tag="PKN80_06650"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNQ35729.1"
                     /translation="DLLSLRGLAREISALLRLPSPALLVHPDRGREASVQPFSIEINA
                     PQACPFYAGALMDGVQVKDFAPARRARLVNLNQRSISNVVDITNYVLLELGQPLHAFD
                     ADRISGGIVVRFARTGEVLTTLDGVERTLNESVLVIADHEKPLALAGIMGGQQSEIGL
                     HTKRILLESAFFNPQVIRMSRKSLGLETEASIRFERGVDPDLVVVSLERAKQLLAEAA
                     GGTVRAAGRAGELKRPSSLIPLHFKHVEQVLGIDIAAEEVESILQGIGCKIEIQKERL
                     GVVPPAYRRDLQEEIDLIEEVARFHDYERLPSRLPRVQILASPDNQLQANRERLRDLM
                     VSAGFDETVSHSLMPVYGREPGLKLINPQSEGQQMLRKSLSASLLRVGLDNLNRGNLK
                     LHLFEIGSVYETGADRSGESQRLAVLLKSGPHDLLQVKALVSEVARRFALEPPEIGGP
                     GCLDDFPGIWLNPPERKNFALVGWHETPFGTAAFAEINLDQDLAKAVPPTRYRTWPKF
                     PAVVRDFSFICPDRVPWAEIQKTVKALSDLIAGVEFFDLYRAPELPENSHSLAFSVRF
                     QSSERTLTRSEVDQLQAQILERLATLGLSLRQ"
     gene            1816..2106
                     /gene="zapA"
                     /locus_tag="PKN80_06655"
     CDS             1816..2106
                     /gene="zapA"
                     /locus_tag="PKN80_06655"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNQ35730.1"
                     /translation="MKKTTVKVRLLGETYLLSGRPEIIREAVRSFTRALETIRRENGE
                     PEVSTHRLGVMAGLALAVEITALRLKKERRLAVLEKARRRLKNAARLLQPPE"
     gene            2181..2252
                     /locus_tag="PKN80_06660"
     tRNA            2181..2252
                     /locus_tag="PKN80_06660"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2213..2215,aa:Met,seq:cat)
     gene            2389..3930
                     /gene="pilM"
                     /locus_tag="PKN80_06665"
     CDS             2389..3930
                     /gene="pilM"
                     /locus_tag="PKN80_06665"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNQ35731.1"
                     /translation="MAKNSILCLDIGTTALKYVSLEETEQGIMLQGIKIIDLPEELEP
                     RRLVEILAGILSETKSKEFALSFSGRYALIRSVAIPAVIGAKVESTLKIEVQHRIPFP
                     LDQVIWSSHRLEESEGMLNFIVGAVKKELVEKLITPFKKFTQEIVFLDADPLVLLNLI
                     KLKHDFNPEKTYALLEMGAESSNLIIFQRSSLLVRALTISGNTFTDNLKEERNIDRSA
                     AEEEKLALTGTDLPLSINTTIDNLLAEIQNSLDYWRFTQKGPEVDLFYLTGGATSMSG
                     FDQVLQNRLRIPCQVLDPLESVRIPEGVSIPTVTKNRLAVAVGLALRTMPSITVPYRL
                     DFLSPDYLRLKESRRNQVFVYLSSLLAIVLAFTPSYFLFMETKVKQSVIEQLNTDLQT
                     YEQYLPRKEDLEREIVDLKGRYESLNNTLNARYLWLSRLIAIGRVLPSEEIYLTRFMP
                     NGDIIELDGDIVSRELSTGFKNIRQFILNLNALPNFQNATVVSLERNQQGILVFKVTV
                     QLKAG"
     gene            3932..>4692
                     /locus_tag="PKN80_06670"
     CDS             3932..>4692
                     /locus_tag="PKN80_06670"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HNQ35732.1"
                     /translation="MEFLRRFRILIIGVLTLMLVGAGVFLDKSLNSQTEALTQEIKGK
                     REKLDKYYADKENAPSPYLIAKLTREKLFLEGNYESLRSRYTLIPVMSLPQDEIFPSL
                     FYKERIYLEIDSLRGRAAKEGINIPANLGLSETGLPPADQIPTLLILLDTIKRVCEEV
                     FKSKLYSLNSLQLSEPVTNPFYVEIPIAISVTANSSRVAYFLENLGVSQNIFVLENMS
                     MNRNGDNVDAQLSLKRILWGKELVDQIKPVGLASQP"
BASE COUNT         1067 a         1454 c         1236 g          935 t
ORIGIN      
        1 gacctgctca gtttaagagg actggcccgg gaaatctccg ccctgctgag gctgccttca
       61 ccggccctgc tggttcatcc ggaccggggc cgggaggcat ccgtccagcc tttcagcatt
      121 gagatcaacg ccccccaggc ctgccccttt tacgccggtg ccttgatgga tggggtccag
      181 gtaaaagatt tcgccccggc ccgccgggcc cggctggtaa atctcaacca gcgttccata
      241 tccaacgtgg tcgacatcac caactatgtt ctgctggaac ttggccagcc tctgcacgcc
      301 ttcgacgccg accggatctc cggcggcatc gtggtcaggt ttgcccgcac cggcgaggta
      361 ctgacgaccc ttgacggcgt cgaacgcact ctcaatgaat cagtcctggt catcgccgac
      421 cacgaaaaac cgctggccct ggccggaatc atgggcggac agcagagcga aatcggattg
      481 cataccaaac ggattctcct ggaaagcgcc ttcttcaacc cccaggtcat ccgcatgagc
      541 cgcaaatcgc tgggtttgga aaccgaagct tcgatccgtt tcgaacgcgg ggtggacccg
      601 gatctggtcg tggtctcgct ggaaagggca aaacaacttc tggcggaagc ggccggcggc
      661 acggtccggg ccgccggtcg ggccggagaa ctgaaaaggc catccagctt gattccgttg
      721 catttcaaac atgtcgaaca ggtgctgggt attgacatcg ccgccgaaga agtggaatcc
      781 attctccagg gaatcggctg caagatagaa atacaaaagg aaagactggg ggtcgtcccg
      841 ccggcctacc ggcgcgatct ccaggaggag atcgacctga tcgaggaggt ggcccgtttc
      901 cacgactacg aacggctgcc cagccggctg ccgcgggtgc aaatcctcgc ctctccggac
      961 aaccagcttc aagccaaccg tgaacgtctg cgcgatctga tggtctcggc cggttttgac
     1021 gaaaccgtca gtcactcttt gatgcccgtc tacggaaggg aaccggggct gaaactgatc
     1081 aacccccaga gcgaggggca gcaaatgctc cggaaaagcc tctccgcttc actgctgcgg
     1141 gtcggattgg acaacctcaa ccggggcaat ctgaaactgc acctcttcga aatcggaagc
     1201 gtttacgaaa ccggcgccga tcgaagcggc gaatctcagc ggctggccgt cttgcttaaa
     1261 tccgggccgc acgatctttt gcaggtcaag gcgctggtaa gcgaagtcgc ccgccgcttc
     1321 gcccttgagc cgccggagat cggcggcccg ggatgccttg acgatttccc cgggatctgg
     1381 ctcaatccgc ccgaacgcaa aaatttcgcc ctggtcgggt ggcatgaaac cccattcgga
     1441 acggcggcct tcgccgaaat caacctggac caggacctgg caaaagcggt tccaccgaca
     1501 cgttaccgaa cctggccaaa atttccggcc gtggtgcgcg atttctcatt catctgcccg
     1561 gaccgggtgc cctgggcgga aatccagaaa acggtcaagg ctctttccga cttgatcgcc
     1621 ggcgtagaat tcttcgatct ctaccgcgcc ccggaactgc ccgaaaattc tcacagcctg
     1681 gccttttcgg tccgtttcca atcttcagaa cgaaccctga cccgcagcga agttgaccag
     1741 cttcaggcgc agatactgga aaggctcgcg accctgggcc tttccctgcg ccaataaaaa
     1801 ggccggccgg cgccgatgaa gaagaccacg gtcaaggtgc gtctcctggg tgaaacctac
     1861 ctgctctccg gacggccgga aataatccgg gaggcggtcc ggtcgtttac cagggcgctg
     1921 gaaacgattc gccgcgagaa cggagagccc gaagtttcca cccaccggct cggcgtcatg
     1981 gccggcctgg ccctggccgt cgaaatcacc gcgctccgcc tgaaaaaaga gcggcggctg
     2041 gccgttcttg aaaaagcccg gcgccgattg aagaacgccg cccgtcttct ccagccgccg
     2101 gaataaccgg cgtcaatcgc agccaaactt gacatccggt cggtaaaaat gctaatttta
     2161 aggacaaaat attgataaac tgcggggtgg agcagtggaa gctcgtcagg cccataacct
     2221 gaaggtcgga ggttcgaatc ctccccccgc tactaaagcg ctactaaaag aaccactccg
     2281 gaaacgtcgg attccggaat cccggcgtaa aagtaaaggt tatcagaaaa cttttacgta
     2341 aagggatgtt ttgtgttttc cggcgcgtgt ccgtcaagga agctttttat ggccaaaaac
     2401 agcatccttt gccttgatat cgggaccacc gccctcaagt atgtcagcct tgaggagacc
     2461 gaacagggca ttatgcttca aggcatcaag atcatcgacc tcccggaaga actcgaaccc
     2521 cgccgcctgg ttgaaatact ggccgggatc ctctccgaaa ccaagtcgaa ggaattcgct
     2581 ctttccttct ccggccgtta cgccctgata cgttccgtcg ccataccggc ggtgatcgga
     2641 gccaaggttg agagcaccct taagattgag gtccagcatc gcattccctt cccgctggat
     2701 caggtcatct ggtcctcgca ccggctcgag gaatcggagg gaatgctcaa cttcattgtc
     2761 ggcgccgtca agaaggaact ggtcgagaaa ttaatcacgc ccttcaagaa gttcacccag
     2821 gagatcgtct tcctggatgc cgatccgctg gttctgctca acctgatcaa gctgaaacac
     2881 gatttcaatc ccgaaaaaac ctacgctctt ctggaaatgg gagccgagag cagtaatctt
     2941 attatatttc aacgttcctc tcttctggtc cgggcgctca ccatctcggg caacaccttt
     3001 accgacaatc tcaaagagga aaggaacatc gaccggtccg ccgccgaaga ggaaaaactg
     3061 gcgctgaccg gaaccgacct tcccctgagc atcaacacga ccatagacaa cctgctggcc
     3121 gaaattcaga actcgcttga ttactggcgc ttcacccaga aaggaccgga ggtcgatctt
     3181 ttttacctga ccggcggcgc cacctccatg tccggtttcg accaggttct gcaaaatcgt
     3241 ctacgcattc cctgccaggt gctggatccg ctcgaaagcg tgcgaatccc ggaaggggtc
     3301 agcataccga ccgtcaccaa gaaccggctg gcggtggcgg tgggcctggc cctgcgcacc
     3361 atgccgtcga tcaccgttcc ctaccgcctc gatttcctct cgccggatta tctgcggctc
     3421 aaggaatccc ggcgcaacca ggtctttgtc tatctttcct ccctgctggc catcgtcctc
     3481 gccttcactc cgagttactt cctcttcatg gaaaccaagg tcaagcagtc ggtaatcgag
     3541 cagttgaata ccgatcttca gacctacgaa cagtacctgc cgcgcaagga agacctggaa
     3601 agggagattg tcgatctcaa gggacgctac gaaagcctga acaatacgtt gaacgcccgt
     3661 tacctctggc tctcccgtct gatcgccatc ggccgggtcc tgcccagcga agaaatctac
     3721 ctgacccggt tcatgcccaa cggtgacatc atcgagctcg acggcgacat cgtttcgcgc
     3781 gagctctcca cggggttcaa aaacatcagg cagttcatcc tgaacctcaa tgcgctgccc
     3841 aatttccaaa atgccacggt tgtttccctg gaacggaacc agcagggaat actggtattc
     3901 aaggtaaccg tacaattaaa agcaggctga catggaattc ctgagacgat tccgcattct
     3961 tatcatcggc gtgctgaccc tgatgctggt cggagccggc gtctttctgg acaagtcgct
     4021 gaacagccag acggaggcct tgacccagga aatcaagggc aaacgcgaaa aactcgacaa
     4081 gtactatgcc gacaaggaaa acgccccctc gccctacctg atcgccaagc tcacccgtga
     4141 aaaactcttt ctcgaaggga attacgagtc gctgcgaagc cgctacaccc tgatcccggt
     4201 gatgtccctg ccccaggatg aaatcttccc ctctttgttc tacaaggaac gcatttacct
     4261 tgagatcgac agcctgcgcg gccgggccgc caaggaaggt atcaacatac ccgcaaacct
     4321 gggcctttcc gaaaccgggc tgccgccggc cgaccagatt cccaccctgc tgatcctgct
     4381 tgatacgatc aagcgcgtct gtgaggaggt tttcaagagc aaactttact cgctgaattc
     4441 actccagcta tccgaaccgg ttaccaaccc cttctacgtc gagataccga ttgccatttc
     4501 cgttacggcc aacagcagcc gggtcgccta cttccttgaa aacctgggcg tctcgcagaa
     4561 tatcttcgtg cttgaaaaca tgtccatgaa ccgtaatggc gacaacgtcg acgcccaact
     4621 cagcctgaaa cgcattctct ggggcaaaga gctggttgac cagatcaagc cggtcggact
     4681 cgcctcccag cc
//
LOCUS       DAOQYN010000156         4664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_18726_length_4664_cov_4.304621, whole genome shotgun sequence.
ACCESSION   DAOQYN010000156 DAOQYN010000000
VERSION     DAOQYN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18726_length_4664_cov_4.304621"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..689)
                     /locus_tag="PKN80_06675"
     CDS             complement(63..689)
                     /locus_tag="PKN80_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNQ35733.1"
                     /translation="MPPVIIVGLITILFFSVIIHEVSHGLAALRNGDDTARVMGRLTL
                     NPLPHIDPFGSLIFPILLVLMRSPVMIGWAKPVPINPFRFRDFQRGMLEVGASGPVSN
                     LVLGTLFALAFRLSGQTAGNGFGLLLLYGVFINYLLALFNLIPVPPLDGSRILGTLLP
                     FRMEAAYYRLERYGMLIVFALVAFGLFDFLVPAVNFLVEIFAGASISG"
     gene            complement(733..2250)
                     /gene="murJ"
                     /locus_tag="PKN80_06680"
     CDS             complement(733..2250)
                     /gene="murJ"
                     /locus_tag="PKN80_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNQ35734.1"
                     /translation="MLKKGFIRAFGVVTLATILVRVAGLAREMTSANYFGTTLVYDAF
                     LLAFMIPNFLRGVLAEGGLNSAFIPVFSEYLGPERRPEAQKVVSDTIGASLLATLALS
                     VLVMLAAGAARFFPISHKAFLILSFLRFTIFYLVFISLAALVMGVLNSLGHFTAPALA
                     PLGLDFFWILSLVLAAGRFNLGLTGRTYCLIGGLLLGGAAQLAIALPAFHRRGFRLRP
                     SLDFRNPALVRMGRLLLPVLVGVAVTPINLMVDYFLASLISPGMVSSLWYANRLVQLP
                     IGVFGVAIATVALPSLSRLAAEGDLPKLKQTLNFSMQLNFLLVIPAALGLVFFREPII
                     RVFFERGLFTSRSTAATGFALLFYSMGLFAYTGGILLTRAFYALRDTVTPVRVGLLSI
                     LVNVGLDLLLMGPLRQGGIALATALVGTLNFFLLYHLLRRRIGPLDGKLVLRKALGVL
                     LISGLAVLAAWRCLRAARHLGPLLSLLAAFAGGLAAYAAAYWLLLHRRYRERPSA"
     gene            2480..3241
                     /gene="hisF"
                     /locus_tag="PKN80_06685"
     CDS             2480..3241
                     /gene="hisF"
                     /locus_tag="PKN80_06685"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNQ35735.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGINFVNLRDAGDPVEIGSAYSNAGA
                     DELVFLDITASHEKRNTILDVVERTAGSVFIPLTVGGGIRSLNDIKETLRAGADKISI
                     NTSAVENPDLIQSGAASYGNQCIVVAIDAKQTGPGRWEVYTYGGRRATGLEAVAWARQ
                     AVELGAGELLVTSMDRDGTRNGYDVPLTRAISEAVPVPVIASGGAGRLEHLAEVLKEG
                     GADAVLAASIFHYGEFTVRQAKKFLATRGIPVRLA"
     gene            3241..4545
                     /locus_tag="PKN80_06690"
     CDS             3241..4545
                     /locus_tag="PKN80_06690"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNQ35736.1"
                     /translation="MSRYLVVLLYNLALLAALPFFLPVSAGRHLRRNGGLRGLRARLG
                     LAAPARRPRISPGAVWIHLVSYGEVVTAAPFVETLLRESGRPAAIWTTTPTGYRAARK
                     AFPELPVNYLPIDLPLNWPFLFGRYRPSVFISLEALFWPNLLIFLDLFRVPAVLINGR
                     LSPRSFRRYLKARPLLAPLLKRVSALGMRSQPEADWIVRLGAPAGRVRATGSLKYDLV
                     IERAARIDPESVRREYGLPGNCLVFGSIHPEEEALALSVSRRLLEEFPGLKIILAPRH
                     LERSRLVELLAESGQAFQAVSSFQTPLAAPFLLLDRMGCLNRAYAAGRAAFVGGSLTI
                     FGDGGHNLVEPAAFGRPVAFGPHAWNFREESGLLLDCGGGVLVRSETELHQFFRRTLL
                     DPAWADAAGNRALEAVRKRTGGVERSVAIVRDLLTNGINEAA"
BASE COUNT          923 a         1489 c         1554 g          698 t
ORIGIN      
        1 agccggcggc ctgaaagatc atcaccaggt caatgaattc catgcttgct ccagcggttg
       61 aatcagccgg aaatggatgc cccggcaaag atttcgacca ggaaattcac ggccgggacc
      121 agaaaatcga agaggccgaa ggcgaccagg gcgaagacga tcagcatccc ataacgttcc
      181 aggcggtagt aggcggcctc catccggaag ggcaacaggg tgcccaggat ccgagaacca
      241 tccagcggcg ggaccgggat gaggttgaag agggccagca ggtagttgat gaagaccccg
      301 tacaggagca aaagaccgaa gccgttcccg gcggtctgac cgctcagccg gaaggccagc
      361 gcgaagaggg ttcccaggac cagattggag acgggcccgg aggcgccgac ctccagcatg
      421 ccccgctgaa aatcccggaa gcggaagggg ttgatcggga ccggcttggc ccacccgatc
      481 atgaccgggc tccgcatcag gacaagcagg atcggaaaga tcaggctgcc gaacggatcg
      541 atgtggggca gcgggttgag ggtcagtcgg cccatgaccc gggcggtgtc gtcaccattg
      601 cggagagcgg ccaggccgtg gctcacctcg tggatgatga ccgagaagaa gaggatcgtg
      661 atcaggccga cgatgattac cggaggcatg caattattat aacctccggc gccggccggg
      721 acaaatggcg ggtcaggcgg acggccgctc ccggtacctt cggtgcagca gcagccagta
      781 agcggcggcg taagcggcca ggccgccggc gaaggcggcc agcagcgaaa gaagcggccc
      841 caggtgccgg gccgcccgca ggcatcgcca ggccgccagg acggccaggc cggagatcag
      901 gagcactccg agcgccttcc gcagcaccag cttcccgtcc agagggccga tccgccgccg
      961 cagcaggtgg taaagcagga agaaattaag ggtgcccacc agggcggtgg ccagggcgat
     1021 ccctccctgg cgcagcggcc ccatcaggag caggtccagc ccgacgttga ccaggatgct
     1081 cagcagcccg acccggaccg gggtgacggt gtcccggagg gcgtaaaagg cccgggtcag
     1141 gagaatgccg ccggtatagg cgaagagtcc catcgagtaa aagagcaggg cgaacccggt
     1201 ggcggcggtg gaacggctgg tgaagaggcc gcgctcaaag aagacccgga taatgggctc
     1261 ccggaagaag accaggccca gggcggccgg gattaccaga agaaagttga gctgcatgga
     1321 aaagttgagg gtctgcttca atttcggcag gtcgccctcg gcggccagcc gggaaagcga
     1381 gggcagcgcc acggtcgcga tggccacccc gaagaccccg atgggcagct gcaccagccg
     1441 gttggcgtac cagagcgaac tgaccatgcc ggggctgatc aggctggcca ggaagtaatc
     1501 gaccatcagg ttgatgggcg taaccgccac gccgaccagg accggcagca gcaggcggcc
     1561 catgcgaacc agggcggggt tccggaagtc cagggagggg cgcaggcgga aaccgcggcg
     1621 gtgaaaagcc ggcagggcta tggccagctg ggcggcgccg cccagcagca gacctccgat
     1681 caggcagtag gtgcgcccgg tcaggcccag gttaaaccgg ccggccgcca gcaccaggct
     1741 taaaatccag aagaaatcca gtccgagggg ggccagggcc ggcgcggtaa aatggccgag
     1801 agagttgagc acgcccatca ccagagccgc cagggagatg aagaccaggt agaagatcgt
     1861 gaagcgcagg aaggagagga tcagaaaggc cttgtggctg atcgggaaga accgggcggc
     1921 gccggcggcc aacatgacca ggaccgagag ggccagcgtg gcaagcaggc tggcgccgat
     1981 cgtgtcgctg acaaccttct gcgcctccgg gcggcgttcg ggccccaggt actcggagaa
     2041 gaccggtatg aaggcgctgt tcaggccgcc ttcggccaac accccgcgca ggaagttggg
     2101 aatcatgaag gccagcagga aggcatcgta aaccagcgtg gtaccgaagt aattggcgct
     2161 ggtcatttcg cgggccagcc cggcgaccct taccaggatg gtagccaggg taaccactcc
     2221 aaaagccctg atgaatcctt tcttaagcat ctggatgccc ggccggcggc gtgaagagat
     2281 gaaaaagcgg ggtccctttt cgggaggtgg tgtctcgatg cgggaaccgg cttgcggtgg
     2341 aaacgtgccc caccttcata ttataaggct tttcaaccgg tttgacaatc accctccttt
     2401 ctggcaaaat aggttttcaa gctgtcccgc ggttcaaccg tgaacttttc ccggaaccca
     2461 accgaagaaa gaggctcgca tgcttaccaa gcgcatcata ccctgcctgg acgtcaagga
     2521 cggccgggtg gtcaagggca tcaacttcgt caacctgcgg gacgccggtg acccggtgga
     2581 aatcggctcg gcttacagta acgccggcgc cgacgagctg gtcttccttg acatcaccgc
     2641 ctcgcacgag aaaaggaata cgatcctcga cgtggtggaa cggacggccg gctcggtctt
     2701 catacccctg accgtgggcg gcggcatccg cagcctgaat gatatcaagg agaccctgcg
     2761 ggcgggcgcc gacaaaatct ccatcaatac cagcgccgtg gaaaaccccg acctgatcca
     2821 gtccggggcg gccagttacg ggaaccagtg catcgtggtg gccatcgatg ccaagcagac
     2881 cggtcccggc cgctgggagg tctacactta cggcggccgc cgggcaaccg gtctcgaggc
     2941 cgtggcctgg gcccggcagg cggtcgagct gggcgccggg gaattgctgg tgaccagcat
     3001 ggaccgggac ggcacccgga acggctacga tgtcccgctg acccgggcga tctccgaagc
     3061 ggtcccggtg ccggtgatcg cctccggcgg cgccggcagg ctcgaacacc tggccgaggt
     3121 gctcaaggaa ggcggcgccg acgcggtcct ggccgccagt atcttccact acggggagtt
     3181 caccgtccgg caggccaaga agttcctggc cacccgcggt atcccggtac gccttgcctg
     3241 atgtcaaggt acctggttgt tctcctctat aacctggctc tgctcgcggc gctgcccttc
     3301 ttcctgccgg tctcggccgg ccgccacctg cgacggaacg gaggcctccg gggcctccgg
     3361 gcgcgactgg gcctggccgc tcccgcccgc cggccgcgga tctcgccggg cgcggtctgg
     3421 atccacctgg tctcatatgg ggaagtggtc accgccgcac ccttcgtcga aaccctgctc
     3481 cgggagagcg gccggccggc ggcgatctgg acgaccactc caaccggata ccgggcggcc
     3541 cgcaaggcct ttccggagct gccggtcaat tacctgccga ttgatcttcc cctcaactgg
     3601 ccgttcctat tcggccgcta ccgcccttcc gttttcatca gcctcgaggc cctcttctgg
     3661 ccaaacctgc ttatcttcct cgacctcttc cgggtaccgg cggtcctcat caacggccgg
     3721 ttgtcgcccc gatccttccg gcgctacctg aaagccaggc cgctcctggc ccccctgcta
     3781 aaacgggtaa gcgccctggg catgcgcagc cagccggagg ccgactggat cgtacgcctg
     3841 ggggcgccgg ccggccgcgt tcgcgccacc ggcagcctca agtatgacct ggtgatcgag
     3901 cgggccgccc gcatcgatcc ggaaagcgtc cggcgggaat acggcctgcc cggaaattgc
     3961 ctggtcttcg gttccatcca ccccgaagag gaggcgctgg ccctctcggt atcccggcgc
     4021 cttctcgaag aattccccgg cctcaaaatc atcctggccc cgcgccacct ggaacgcagc
     4081 cggctggttg aactgctggc ggaatccggc caggccttcc aggcggtcag ttccttccag
     4141 accccgctgg ccgccccctt tctcctgctg gaccggatgg gctgcctgaa ccgggcttac
     4201 gcggccggcc gggccgcctt cgtgggcggc agcctgacca tcttcggaga cggcggccac
     4261 aacctggttg aaccggccgc ctttggccgg ccggtcgcct tcggaccgca cgcctggaac
     4321 ttccgggaag agtccgggct ccttctggat tgcggcggcg gcgtcctggt ccgatcggaa
     4381 acggaactcc atcaattctt tcgccggacg ctcctggatc cggcctgggc cgacgcggcc
     4441 ggaaaccggg ccctggaagc ggtgcgaaag cgtaccggcg gggtggaaag atcggtggcg
     4501 atcgtccggg atttattgac caacggcatc aatgaggcgg cttgaagatt attgacatgc
     4561 caactcaaga atccggcggc gaaatgacgg agcccaaacc caacctcggc gattttaccc
     4621 tggaagaact gaccgcccgc ctctccgaac tgggcgagcc gacc
//
LOCUS       DAOQYN010000157         4660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_18755_length_4660_cov_4.573507, whole genome shotgun sequence.
ACCESSION   DAOQYN010000157 DAOQYN010000000
VERSION     DAOQYN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18755_length_4660_cov_4.573507"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2099)
                     /locus_tag="PKN80_06695"
     CDS             complement(<1..2099)
                     /locus_tag="PKN80_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNQ35737.1"
                     /translation="MANLLVPLLLVAAAASAQAGEPPPIPGAGYRLIDDFAYPDTAAL
                     NEAWQPMRGSPPAAVVNAGGGRALNLSCRFRATEIERASWDRRVRLDLTAVRGLRFLF
                     FSPDTAPVSHFSFYLRSGNGWYAARFAPKVKNGWTEVRIDKAETLIEGQPAGWSAIDA
                     LRISAWRGEVADTGFYIANLGFWEEASRIVVIRGESVAGSAAGETGSVERYAKSLAGR
                     LDELGLPYTLISDLDVTGERLRGTKLVILPHNPRLPESTAEILADFISGGGKLLSFYT
                     PLTGRLAEAAGMRPGPHIGETYRGQFASIRPLGPELPGAPAEVKQRSWNIRRYEPVPG
                     RGRVAAAWFNDRGEPTGEPAVLVSERAAHLTHVLLADDPDNQRMLLLAMLGRLVPEFW
                     ETAARSGLDAVGRFGPFGGWEEAAAALRTRSAGNRSARGELERAAGLRAEARRLAAGQ
                     NFAGAVVRAREAQAAAVAAYGAVQRPVPGEHRAWWCHSAFGPAGMDWDEAVRVLAENG
                     FTAILPNLAWGGLAYYESRVLPVAAEVKERGDQLVQCLAACRKYGVACHVWKVNWNLG
                     GRAPREFAERLKREGRTQIGYDGKALDGWLCPSHPANRQLEIEAMAELAERYEVAGIH
                     FDYIRYPDQQSCFCAGCRERFEAAIGAKVDNWPRDTREREDIRKKWLDFRRDNITSVV
                     AGVSERVRPRKPGVKISA"
     gene            complement(2209..3573)
                     /locus_tag="PKN80_06700"
     CDS             complement(2209..3573)
                     /locus_tag="PKN80_06700"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35738.1"
                     /translation="MVNVCFLGSGLAWTPRLLIDLVSVFKDEKLDIRLVDINPENADL
                     IARYGAVVNRQHGRRDRYRPLADRRAGLKGADAVIIALSTGGLDAMEQDIAIPEKYAI
                     FATVGDTAGPSGWSRAVRNIPVFAAFAEDLEAICPKAFVTNYTNPMSALSATLQLGCS
                     NPSVGLCHAYFETKDVIKEIFGLPDWSRISISVAGMNHFTWVVDFKIGREDGYRLLRE
                     RIGRRGSLRDVLPKESTDEIGFTSRSALCCDLYDAFGYLPYPADRHTSEFVSFALCGR
                     PERTTHRDKDGNQFEAIRYCAIRRTAVAARKKGMANRPRNVEAYISGRTPLPTRTRET
                     GIDMIAAYLYNRPLFDVANLLNHGQVPGLPADACVETLAMIDGLGVRPLLVDRVPEPL
                     LEIMRPQAVNQKWTVEGVLKKDRDLLFQALYNDPMCRHLKPGEIRRMGEELLKANRRW
                     FKLP"
     gene            complement(3611..>4660)
                     /locus_tag="PKN80_06705"
     CDS             complement(3611..>4660)
                     /locus_tag="PKN80_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNQ35739.1"
                     /translation="YTYTSTLHYVMEAAAELGREKRLAEPIPKLAVLVAASPFDAALH
                     DAYGKVHGLNVYNAYGPEFMNRDLAAYLTGEFAGEYLDRYTLRQPKEKMPLYHLIGAL
                     DPLAEADIKARLNDGYPETLPEWINRDGLTHLKIKLNGSDLDWDVNRVLAIDRTCREV
                     QAARGVEQWYYSADFNERCPNVRYLLDFLARVKAGSAGAFERIQYIEQPTSRHLKEDR
                     ANKMHEAARVKPVVIDEALVDFEHLQLAMEMGYSGVALKTCKGHTQALLMAAAALKYG
                     LFLCVQDLTLVGESYLHSIGLGARVPKIAAVEGNGRQYCPVANTEGLKKWPEAFTVKQ
                     GSIVTANLVRPGLGH"
BASE COUNT          718 a         1639 c         1567 g          736 t
ORIGIN      
        1 gccgagattt tgacgccggg cttccgcggc cgcacccgct cgctcacccc ggctacgacg
       61 ctggtgatgt tgtcgcggcg gaaatccagc cacttcttcc ggatatcctc ccgctcccgg
      121 gtatcccggg gccagttgtc aaccttcgcc ccgatcgccg cctcgaaacg ctcccggcag
      181 ccggcgcaga agcagctctg ctgatccggg tagcggatgt aatcgaagtg gatgccggca
      241 acctcgtaac gctcggccag ctccgccatc gcctcgatct ccagctgccg gttggccggg
      301 tgcgacgggc agagccaccc gtccagcgcc ttcccgtcat acccgatctg ggtccggcct
      361 tcccgcttca ggcgttcggc gaactcccgg ggcgcccggc cgcccaggtt ccaattcacc
      421 ttccagacat ggcaggcgac cccgtacttc cggcaggccg ccaggcattg cacaagctgg
      481 tcgccgcgct ccttcacctc ggccgccacc ggcagcaccc ggctctcgta ataggccagc
      541 ccgccccagg ccagattggg caggatggcg gtaaacccgt tttcggccag gacccggacc
      601 gcctcgtccc aatccattcc cgccggacca aaggcgctgt ggcaccacca ggcccggtgc
      661 tcgccgggaa ccggccgctg cacggcgccg taagcggcca ccgccgccgc ctgggcctcc
      721 cgcgcccgga cgaccgcccc ggcaaaattt tgcccggccg ccagccgtcg ggcctcggcg
      781 cgcaagccgg ccgcgcgctc cagttcgccg cgggcggacc ggttgccggc cgaccgcgtc
      841 cgcagtgccg cggccgcctc ctcccaaccg ccgaacggac cgaagcggcc gaccgcgtcc
      901 aggccggacc gggccgccgt ctcccagaat tccggaacca gccgccccag catggccagc
      961 agcagcatcc gctggttatc cggatcgtcc gccagcagaa cgtgcgtcag gtgggcggcg
     1021 cgctcagaga ccaggacggc cggctcgccg gtcggctcgc cccggtcatt gaaccaggcg
     1081 gcggccacgc ggccgcgccc cggcaccggc tcgtagcgcc tgatgttcca ggaccgctgc
     1141 ttcacctcgg ccggcgcgcc cggcagttcc gggccgagcg gacggatcga agcgaactgg
     1201 ccgcggtagg tctcgccgat atggggaccc ggccgcatcc cggcggcctc ggccagccgg
     1261 ccggtcaacg gggtgtaaaa ggaaagcagc ttgccgccgc ccgagatgaa atcggccagg
     1321 atttcagcgg ttgactccgg caggcgcggg ttatgcggca ggatgaccag cttcgttccc
     1381 cgcagccgtt cgccggtgac atccaggtcg ctgatcagcg tgtaaggcag gcccagttca
     1441 tcgagccggc cggccaggct cttcgcgtac cgctcgacgc tcccggtctc cccggccgcg
     1501 ctcccggcga ccgactcgcc ccggatgacc acgatccggc tcgcttcctc ccagaaaccg
     1561 aggttggcga tgtaaaaccc ggtgtcggcc acctccccgc gccaggccga gatgcgcagg
     1621 gcgtcaatgg ccgaccagcc ggccggctgc ccctcgatca gggtttcggc cttgtcgatc
     1681 cgaacctcgg tccagccgtt tttgaccttc ggcgcgaacc gggcggcata ccagccgttg
     1741 ccgctgcgca ggtaaaagga aaagtgggag accggcgcgg tatcgggaga gaagaacagg
     1801 aagcgcaaac cccggacggc ggtgaggtcc agccgcaccc gccggtccca ggaggcgcgc
     1861 tcgatctccg tcgcccggaa acggcaggac aggttgagcg cccggccgcc gccggcgttg
     1921 acgacggctg ccggcgggct gccccgcatc ggctgccagg cctcgttcag ggccgccgtg
     1981 tccgggtagg cgaaatcgtc gatcagccga taaccggccc cgggaatggg cggcggttcc
     2041 ccggcctggg ccgacgcggc ggccgccacc aggagaagcg ggaccaggag gttcgccaac
     2101 ccgcctcgaa accgggaccg gaaaaacccc cttttcattg gcatcttgat ccccctcacg
     2161 acccgcgcct ggccgccgag gcaaggaaac cagggaagcg gccgcacctt acggcagttt
     2221 aaaccagcgc cggttggcct tcagcagctc ctcgcccatc cggcggattt cgcccggctt
     2281 gaggtggcgg cacatcgggt cattgtaaag ggcctggaag agcaggtccc gatccttctt
     2341 cagcaccccc tcaaccgtcc acttctggtt gaccgcctgg gggcgcatga tctccagcag
     2401 cggctccggc acccggtcca ccagcagcgg ccgcacgccc aggccgtcga tcatggccag
     2461 cgtttcgacg caggcgtcgg ccggcaagcc ggggacctgg ccgtggttga gcaggttggc
     2521 cacgtcgaag agcggccggt tgtaaaggta ggcggcgatc atgtcgatgc cggtctcccg
     2581 ggtccgggtc ggcagcggcg tccggccgga aatgtaggcc tcgacattgc ggggccggtt
     2641 ggccatgccc ttcttgcgcg cggcgaccgc ggtgcgccgg atggcgcagt acctgatggc
     2701 ctcgaactgg ttgccgtcct tatcccggtg cgtcgtccgc tcgggccggc cgcagagggc
     2761 gaaggagacg aactcgctgg tgtgccggtc ggccgggtaa ggcaggtagc cgaaggcgtc
     2821 gtagagatcg cagcagaggg cgctgcggga ggtgaagccg atctcgtcgg tgctctcctt
     2881 cgggagcacg tcccgcaggg agccgcgccg gccgatgcgt tcacgcagca ggcggtagcc
     2941 gtcctcccgg ccgatcttga aatcgaccac ccaggtgaag tgattcatgc cggccaccga
     3001 gatcgagatc cggctccagt cgggcaggcc gaagatctcc ttgatcacgt ccttggtctc
     3061 gaagtaggcg tggcagaggc cgacgctcgg gttggagcag cccagctgca gggtggccga
     3121 gagggccgac atcgggttgg tgtagttggt gacgaaggcc ttcgggcaga tcgcctccag
     3181 gtcctcggcg aaggcggcga aaaccgggat gttccggacc gcgcgcgacc agcccgaggg
     3241 gccggcggtg tcgccgacgg tggcgaagat ggcatacttc tccgggatgg cgatatcctg
     3301 ctccatggcg tccaggccgc cggtgctcag ggcgataatc accgcatcgg cccccttcag
     3361 gccggcccga cggtcggcca gcggccggta gcggtcccgg cggccgtgct gccggttgac
     3421 caccgcaccg tagcgggcga tcaggtcggc attctcaggg ttgatgtcaa ccagccggat
     3481 gtccagcttc tcatccttga agacgctgac caggtcgatc agcagccgcg gggtccaggc
     3541 cagcccggaa cccaggaaac agacgttgac catttcgtta ccctcccttg atgagcgcgg
     3601 acgcttttct tcaatggccc agtccgggcc ggaccaggtt ggcggtgacg atgctgccct
     3661 gcttgaccgt gaaggcctcc ggccacttct tcagcccctc ggtgttggcg accgggcaat
     3721 actggcggcc gttgccctcg accgcggcga tcttcggcac ccgggccccc agcccgatcg
     3781 agtgcaggta cgactcgccc accagcgtca ggtcctggac gcagaggaag agtccgtact
     3841 tcagggcggc cgccgccatc aggagcgcct gggtgtgccc cttgcaggtc ttcagggcca
     3901 cgccgctgta ccccatctcc atggccagct ggagatgctc gaaatcaacc agggcctcgt
     3961 cgatgaccac cggcttcacc cgggcggcct catgcatctt gttggcccgg tcctccttca
     4021 ggtggcgcga ggtgggctgc tcgatgtact ggatgcgctc gaaggcgccg gccgaccccg
     4081 ccttgacccg ggccaggaaa tccagcaggt accggacgtt cggacagcgc tcgttgaaat
     4141 cggcgctgta gtaccactgc tcgaccccgc gggccgcctg gacctcccgg caggtccggt
     4201 cgatcgccag gacccggttc acgtcccagt ccaggtcgct gccgttgagc ttgatcttca
     4261 ggtgggtcag gccgtcccgg ttgatccatt cgggcagggt ctccgggtag ccgtcgttaa
     4321 ggcgcgcctt gatgtcggcc tcggccagcg ggtcgagggc gccgatcagg tggtagagcg
     4381 gcatcttctc cttgggctgg cgcagggtgt agcggtccag gtattcgccg gcgaactccc
     4441 cggtcaggta ggcggccagg tcccggttca tgaactccgg cccataggcg ttgtagacgt
     4501 tcaggccgtg aaccttgccg taggcgtcgt ggagggcggc gtcgaagggc gaggcggcca
     4561 ccagcacggc cagcttggga atcggctcgg ccagtctctt ctcccggccc agctcggcgg
     4621 cggcctccat cacgtagtgc aaggtgctgg tgtaggtgta
//
LOCUS       DAOQYN010000158         4633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_18918_length_4633_cov_4.576016, whole genome shotgun sequence.
ACCESSION   DAOQYN010000158 DAOQYN010000000
VERSION     DAOQYN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18918_length_4633_cov_4.576016"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PKN80_06710"
     CDS             <1..518
                     /locus_tag="PKN80_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNQ35740.1"
                     /translation="SARVLLPEREATAVEIALNSLTREIKEREPFHREVARTELEKVL
                     WLAHRFGRRPAGRPPAHPRLRKVIEYLDDNFADDLGLEELADRFALSAGHLSRTFKRY
                     TGLGLKQYILQRRIVAAKRLLEEAPGLKVAAVSNRVGFHDFAVFNRDFKLITGLTPSA
                     YRKNSYPAVKK"
     gene            606..1928
                     /locus_tag="PKN80_06715"
     CDS             606..1928
                     /locus_tag="PKN80_06715"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ35741.1"
                     /translation="MSKLALFGGPRAVSSDYREIFAWPIVNAEMESAVLEVLRNGNMS
                     GSNLTRQFERAFADWHGRTYGLGCNNGTASLHCAMFGLKIGPGDEIICPSITYWASCL
                     PVYSLGGTVVFADIDPKTLCLDPDDIEARISKHTKAILVVHYCGRPADMDRITAVARK
                     HKLAVIEDVSHAHGALYKGRLVGTFGAAAGFSLMSGKSFAIGEAGIMLTDSQEVYERA
                     AAFGFYERSGELTLPELKAGAGLPWGGYKYRMHQMSSAVGLVQLKKYPAEMAEIDRAM
                     NYFFDRLEGTPGIRSFRPKGEPGSTMGGWYNPMVGYVPEELGGLSVTRFCQSLLAEGF
                     KTSPGCNSALHLHPLFNTVDVYGHGRPTRIANSDRDLRQPPGTLPVSEGLQSRVFKAP
                     WFKRFDTKAIDEIVTAIKKSAENYRELLADDPGNPEIMGGAGLTQLVR"
     gene            1988..3898
                     /locus_tag="PKN80_06720"
     CDS             1988..3898
                     /locus_tag="PKN80_06720"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNQ35742.1"
                     /translation="MVRTRKKLPGLCRSAALFLLLLAAFPAGAGGQALPYNTVLCGRT
                     EGVITVDGRLDEPAWAQAPATPVFTDLATGAAAAFPTFAKFLRDRQNLYVAYEVADTR
                     IWSECAKRDVNLYCSTDRGLYNEGFIELFIDPDDDGRNQIEIHVNPQNAVTDQYKTYC
                     TSWYTRTKLGLPHFRAPANQATWDLAGMRTAVRLEGTLNKHDDTDRGWTVEIAIPFAS
                     LKEFAGGQSLPPREGDSWRINVCRRYRLERGAPVEYWSWPVLNSNNCHRADRWGYLLF
                     EDRPAGRAARLENLPPARFDWKALWAKPPRSRAQAQQLARLADDLGFNVIILQATGYN
                     QAWYRSSILKPAKGLTIDPLAEVIRAAHPRGIKVYAWVIYLADSGSDAMRAKAPEFYQ
                     KLKPGTAADTGPRRNPDRENLMAGDWLCPDRGLTDHEKKISLEIVTRYPVNGLAIDYL
                     GYRNYRDCHCEYSVRRRAEFERRLIAKEGFYKPWSATMVADEFSEQSLIDWTEELVAA
                     VKAVRPGLKTAIHLYPDFDPNPLYGHRLPVDYLGQTVAWFYQPYWSPEKIYYRTRLHQ
                     SLSSRYQRKSRFVPFVGVAKGKLLKSPEQVRQELRIAGNFGNGAVMFAFSDVLLENPA
                     LAEAVKKELKKR"
     gene            3952..>4633
                     /locus_tag="PKN80_06725"
     CDS             3952..>4633
                     /locus_tag="PKN80_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNQ35743.1"
                     /translation="MALLHINYWSAPLGKQAALYFILPQGKETEPCRVVYLLHGRSDD
                     YTIWLRRTSLERYAEATGLAVALLDGARSFYTDSFDGVFKYEQHILETVNYLDKTFHT
                     VRSARGRGIGGLSMGGYGAVKLGFKHPDLFGSITAHSSVLDLAGYIKKHPSPELKAIF
                     GPRSKAGEDPFRLAARVKQIPALYFDCGLDDPLLEDNRRFASRLKELGLPHTYREYPG
                     AHTWEYWDR"
BASE COUNT          895 a         1588 c         1380 g          770 t
ORIGIN      
        1 tctccgcccg ggtgctcctg ccggaacggg aagcgactgc ggtggagatc gccctcaaca
       61 gcctgacccg ggaaatcaag gaacgggaac cttttcaccg ggaggtggcc cggaccgagc
      121 tggagaaggt gctctggctg gcccaccgct tcggccggcg gccggccggc cgaccgccgg
      181 cccacccccg gcttcgaaaa gtaatcgaat acctggacga caatttcgcc gacgacctcg
      241 gcctggagga gttggccgac cgcttcgctc tttcggccgg ccacctttcg cggaccttca
      301 aacgctacac gggcctggga ctgaaacagt acattctcca gcgccggatc gtggccgcca
      361 agcgccttct ggaagaggcg cccgggttga aggtggcggc cgtctccaac cgggtcggtt
      421 ttcacgactt cgccgtcttc aaccgggatt tcaagctcat caccggactc accccatcag
      481 cctaccgcaa gaattcatac ccggccgtca aaaaatagta ttgtattgca ttcccctttc
      541 ggctatcatt aataagaagc gagcggccgc cgctcgttac cgaaaaaatt caaggaggca
      601 tggccatgtc aaaactggcg ctcttcggcg ggccccgggc tgtaagttcc gactaccggg
      661 aaatcttcgc ctggccgatc gtcaacgccg agatggaatc ggcggttctg gaggtcctgc
      721 gcaacggcaa catgtcgggc agcaatctca cccggcagtt cgaacgggcc ttcgccgact
      781 ggcatggtcg gacctacggg ctgggctgca acaacggcac cgcctccctg cactgcgcca
      841 tgttcggcct gaagatcggc ccgggcgacg aaatcatctg cccgagcata acctactggg
      901 cctcctgcct gccggtctat tcgctcgggg gaacggtggt tttcgccgac atcgacccca
      961 aaacgctctg tctcgacccc gatgacatcg aggcgcgcat cagcaagcat accaaggcca
     1021 tcctggtggt ccattactgc ggccggccgg ccgacatgga ccgcatcacg gccgtcgccc
     1081 gcaagcacaa gctggccgtc atcgaggacg tttcccacgc ccacggcgcc ctttacaagg
     1141 ggcggctggt cggaaccttc ggcgcggcgg ccggcttctc gctgatgtcg ggcaaatcct
     1201 ttgccatcgg cgaagccggc atcatgctca ccgacagcca ggaggtctac gaacgggcgg
     1261 ccgccttcgg tttctacgag cgcagcggcg aactcactct cccggaactc aaggccggcg
     1321 ccggcttgcc ctggggcggc tacaagtacc gcatgcacca gatgtcctcg gcggtcggcc
     1381 tggtccagct gaagaaatac ccggccgaga tggccgagat cgaccgggcc atgaattact
     1441 tcttcgaccg gctggaaggc acgccgggca tccggtcctt ccgccccaag ggtgaaccgg
     1501 gcagcaccat gggcggctgg tacaacccga tggtcggata cgtgcccgag gagctgggcg
     1561 gcctttcggt gacccgtttc tgccagtcgc tgctggccga aggattcaaa accagcccgg
     1621 gctgcaactc cgccttgcac ctgcatcccc ttttcaacac cgttgatgtt tacggccacg
     1681 gccggccgac ccggatcgcc aattccgacc gcgacctgcg ccagccgccg ggaacgctgc
     1741 cggtctccga agggctccag agccgggtct tcaaggcccc ctggttcaaa cgcttcgaca
     1801 ccaaggccat tgatgaaatc gtgacggcca tcaaaaagag cgccgagaat taccgggaac
     1861 tgctggccga cgatccgggc aacccggaga tcatgggcgg cgcgggactc acccagctgg
     1921 tacggtagcc gcggccaggc cgccagggca ggcgcctctt tcaacccctg ccaataagga
     1981 gaaatatatg gtccggacca ggaagaaact gccgggactc tgcagaagcg ccgcgctgtt
     2041 cctgctgctg ctggcggcct ttccggccgg cgccggcggc caggcgctgc cttacaacac
     2101 cgtcctctgc ggccggaccg aaggcgtgat caccgtggac ggccgcctgg acgagccggc
     2161 ctgggcgcag gccccggcca cgccggtctt cacggacctg gccaccggcg cggcggcggc
     2221 cttcccgacc ttcgccaaat tcttgcgcga ccggcagaat ctctacgtgg cctacgaggt
     2281 ggccgacacc cggatctggt ctgaatgcgc gaaacgggat gtaaacctct actgctctac
     2341 cgaccggggc ctttataatg aaggattcat agaactgttt atcgatccgg acgacgacgg
     2401 ccgcaaccag atcgagatcc acgtcaaccc ccagaacgcg gtcaccgacc agtacaagac
     2461 ctactgtacc agctggtaca cccggaccaa actcggcctg ccacacttcc gggccccggc
     2521 caaccaggcg acctgggacc tggccggaat gcggaccgcg gtgcgcctgg agggcacgct
     2581 caacaaacac gatgacaccg accggggctg gacggtggag atcgccattc cattcgcctc
     2641 gcttaaggag ttcgccggcg gccagtccct gccgccccgc gagggcgaca gttggcggat
     2701 aaacgtctgc cgacgttacc ggctggaacg gggcgcgccg gtcgaatact ggagctggcc
     2761 ggttctgaat tccaataatt gtcaccgggc ggacaggtgg ggttacctgc tcttcgagga
     2821 ccggccggcc ggccgggcgg cccggctgga aaacctgccg ccggcccggt ttgactggaa
     2881 ggctctctgg gccaagccgc cccggagccg ggcccaagca cagcagctgg cccggctggc
     2941 cgacgacctg ggcttcaacg tcatcatcct tcaggcgacc ggctacaacc aggcctggta
     3001 ccgcagcagc attctcaagc cggccaaagg attgaccatc gacccgctgg ccgaggtgat
     3061 ccgggccgcc cacccgcgcg gcatcaaggt ctacgcctgg gtcatctacc tggccgacag
     3121 cggctcggac gccatgcgcg caaaggcgcc ggaattctac cagaaactga agcccggcac
     3181 cgcggccgac accgggccgc gccgcaaccc ggaccgtgag aacctgatgg ccggcgactg
     3241 gctctgcccg gaccggggcc tgaccgacca cgaaaagaag atcagcctgg aaatcgtcac
     3301 ccgctacccg gtcaacggcc tggccatcga ctacctcggc taccggaatt accgggactg
     3361 ccactgcgag tactccgtcc gacgtcgggc cgagttcgaa cgccggctga ttgccaaaga
     3421 gggtttttac aagccctggt cggcaaccat ggtcgccgat gaattctccg agcagtcgct
     3481 gatcgactgg accgaggagc tggtggcggc ggtcaaggcg gtccggcccg gcctcaagac
     3541 cgccatccac ctttacccgg actttgaccc caacccgctc tacggccacc ggctgccggt
     3601 cgattatctc ggccagacgg tcgcctggtt ctaccagccc tactggagcc cggagaaaat
     3661 ctactaccgg acgcgcctgc accaatccct ctcgagccgc taccagcgca agagccgctt
     3721 cgtgcccttc gtcggcgtgg ccaagggaaa gctgctcaag tccccggagc aggtccgaca
     3781 ggagctgcgg atcgccggca acttcggcaa cggcgcggtc atgttcgcct tctccgacgt
     3841 cctgctcgaa aacccggccc tggccgaggc cgtcaaaaag gagttgaaaa aacggtagta
     3901 cccggaccgg ccggcgaaaa caggccggca tcacgctaac cccaaaaaac catggctttg
     3961 ctacacatca actactggtc agccccgctc gggaaacagg ccgcccttta tttcatcctg
     4021 ccccagggca aggagacgga accctgccga gtcgtttacc ttctccacgg acgcagcgac
     4081 gattacacca tctggctgcg ccgcaccagc ctggaaaggt atgccgaagc gaccggcctg
     4141 gcggtcgccc tgctcgatgg tgcccgttca ttttacaccg acagttttga cggcgtcttc
     4201 aaatacgagc agcacatcct ggagaccgtc aattatctcg ataagacctt ccataccgtt
     4261 cgctccgccc ggggccgggg catcggcggc ctctcgatgg gcggctatgg cgcggtgaag
     4321 ctcggtttta agcatccgga cctcttcgga tcgatcaccg cccattcctc ggttcttgac
     4381 ctggccgggt acatcaaaaa acacccttcc ccggaattga aggccatctt cggtccccga
     4441 tcaaaggccg gtgaagaccc tttccggctg gcggcccgag tcaagcagat accggccctt
     4501 tacttcgact gcggcctgga tgatccactg cttgaggata accggcgctt cgcctcccgg
     4561 ctcaaggagc tggggctgcc ccacacctac cgggaatacc cgggcgccca cacctgggaa
     4621 tactgggacc ggt
//
LOCUS       DAOQYN010000159         4582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_19199_length_4582_cov_4.870775, whole genome shotgun sequence.
ACCESSION   DAOQYN010000159 DAOQYN010000000
VERSION     DAOQYN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19199_length_4582_cov_4.870775"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..804
                     /locus_tag="PKN80_06730"
     CDS             37..804
                     /locus_tag="PKN80_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNQ35744.1"
                     /translation="MKINFGDNNYEELQAAIEKEAMVLVPVGQVEEHGPHLAVKTDAL
                     IAERLCAAAARELAPEFPVLVTPTVWSGYSTREVLRWPGCLTLRLTTLIEMMYDISAG
                     LVRMGFKKAALVNCHGNHPGALEVVVRRIADDFGVYLALTNPMLLARPEIMAILEKGA
                     AGSCHAGEFETSVMLYLDPDSVRMEKASDVDILRIKEGLSGPGAFVSTWGVQKSLTGV
                     FGAPRTATAAKGEALFRALVEGQKKFLRSYARLEPAA"
     gene            complement(847..4059)
                     /gene="carB"
                     /locus_tag="PKN80_06735"
     CDS             complement(847..4059)
                     /gene="carB"
                     /locus_tag="PKN80_06735"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNQ35745.1"
                     /translation="MPKRTDLKKILLIGSGPIIIGQACEFDYSGSQACKALKEEGYRV
                     VLVNSNPATIMTDPGLADATYIEPLSIEFLTRVIEKERPDALLPTLGGQVGLNLATFL
                     AREGVLEKYGVEMIGADAEAIARAETRDLFKKTMQAIGVEVPASGLAYSVEDGIAIAE
                     KIGFPLILRPAYTLGGTGGSTVYNLEELKAALAKGIEASPIGEVLVEESVLGWKEIEF
                     EVMRDCADNTIIVTSMENVDPMGVHTGDSIVVAPAQTLTSGEYGRFAELCRRIIRAIG
                     INGGGANIQFGLNPENGRIVIIEVNPRLSRSSALASKATGFPIARIATKLAVGFTLDE
                     VRNEITGRTSAFYEPTVDYCVFKIARFTFEKFAGADPTLNTSMKSVGEVMSIGRTFKE
                     SLQKGLRALETGRFGLGADGKDGPEPDRETVAERLRVPNGERLFYLRHAIRQGFSTNE
                     IYRLSRIDPWFIGQIRELVEAEAGFKRLDRRSLRRAKEYGFSDRQLACLSGLTEVQVR
                     SRRKKLGIEPVFKLVDTCASEIEVARPYFYSSYDRGDECRADRKRKVAILGGGPNRIG
                     QGIEFDYCCVHASFALREAGYQSIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLNIVE
                     RERPLGAIVQFGGQTPLNLAIPLQRSGLKILGTSPDSIDRAEDRKRFSRLLNSLRLQQ
                     APNGTASSFEAARKIAESIGYPVLVRPSYVLGGRAMEIVYDTGSLTEFMAKAVEVAPE
                     HPVLIDKFLEDAIEVDVDAICDRRQVVVAGVMEHIEEAGIHSGDSAMVLPTYSLSRRL
                     VEEIKEATGKLALALKVCGLINIQFAVKENRLYVLEVNPRASRTVPFVSKVIGVPLAN
                     LATKLMLGSSLEELGFTREVPVSHFAVKESVFPFNRFPGVDTVLGPEMRATGEVMGLD
                     SSFERAYAKSQLAAGQKLPRSGRVFLSVRDRDKSALVPVARGLHELGFELLATAGTGR
                     RLAEAGVPVRIIPKVSEGRPNIVDHIKDGEIALVINTPSGQKPRRDITTIRAIAVSRG
                     VPLITTMPGAKATFLAIKELKQGELSVRSLQEYHAAIRRA"
     gene            complement(4052..>4582)
                     /locus_tag="PKN80_06740"
     CDS             complement(4052..>4582)
                     /locus_tag="PKN80_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HNQ35746.1"
                     /translation="FVAYDFGARADSLRQLAALGGRVTLVPFDTPAEAVLELKPDAFF
                     LSSGPGDPAGLSESVAAVRTLSEKLPVLAFGLGFQVLCLALGAAAFRMKTGHYGLNQP
                     VQDLAGRRCLSTSQCHSLAIDPASLGPGLQVTHLNLNDRTVEGVRHRERPLAGLQYYP
                     ANDDPIFETFAGFLNA"
BASE COUNT          712 a         1430 c         1674 g          766 t
ORIGIN      
        1 tatttccaaa aaaaggctta aaccgaggag gtcgggatga agataaattt cggcgacaac
       61 aattacgagg agttgcaggc ggccatcgag aaggaggcga tggtgctggt gccggtgggg
      121 caggtcgagg agcacgggcc gcacctggcg gtgaagaccg acgccctgat cgccgagcgg
      181 ctctgcgccg cggcggcccg ggaactggcc ccggagttcc cggtcctggt gaccccgacg
      241 gtctggagcg gctattccac ccgcgaggtg ctgcgctggc ccggctgcct gacgctgcgg
      301 ctgaccaccc tgatcgagat gatgtacgac atcagcgccg gcctggtgcg gatgggcttc
      361 aagaaggcgg cgctggtcaa ctgccacggc aatcacccgg gcgcgctgga ggtggtggtg
      421 cgccggatcg ccgacgattt cggggtttac ctggccctga ccaacccgat gctcctggcc
      481 cggccggaga tcatggcgat cctggagaag ggggcggcgg ggagctgcca cgccggcgaa
      541 ttcgagacct cggtgatgct ttacctcgac ccggactcgg tccggatgga gaaggccagc
      601 gacgtcgaca tcctgcggat caaggaggga ttgtccggcc cgggggcctt cgtttcaacc
      661 tggggagtgc agaagagcct gaccggcgtc ttcggggcgc cccggaccgc caccgccgcc
      721 aagggcgagg ccctcttccg ggcgctggtc gaggggcaga agaagttcct gcgcagttac
      781 gcccgcctgg aaccggcggc ctgagccgcc gccctacccg tttttttcgc ggccggccgg
      841 cgccggtcag gcgcggcgga tggccgcgtg gtactcctgg aggctccgga ccgaaagctc
      901 gccctgcttc agctccttga tggccaggaa ggtcgccttg gcgccgggca tggtggtgat
      961 gagcggcacg ccgcggctca ccgcgatggc ccggatggtg gtgatgtcgc gccggggctt
     1021 ctggccggac ggggtgttga tgaccagggc gatctcgccg tccttgatgt ggtcgacgat
     1081 gttcggccgc ccctcggaga ccttggggat gatccggacc ggaacgcccg cctcggccag
     1141 ccgccggccg gtgccggcgg tggccagcag ctcgaagccg agttcgtgca ggccgcgggc
     1201 caccggtacc agcgcggact tgtcccggtc ccggacgctc aggaagaccc ggccggagcg
     1261 gggcagcttc tggccggcgg ccagctggct cttggcgtag gcgcgctcga aggaggagtc
     1321 gaggcccatc acttctccgg tggcacgcat ctccggaccc agcaccgtgt cgacgcccgg
     1381 gaagcggttg aaggggaaga ccgattcctt gaccgcgaag tgggagaccg ggacctcgcg
     1441 ggtgaagccg agttcctcga ggctgcttcc gagcatcagc ttggtggcca ggttggccag
     1501 cggcaccccg attaccttgc tgacgaaggg gacggtccga ctggcgcgcg ggttgacttc
     1561 caggacgtag aggcggttct ccttgacggc gaactggatg ttgatcaggc cgcagacctt
     1621 cagggccagg gccagcttgc cggtggcctc cttgatctcc tcgaccagcc ggcgggagag
     1681 ggagtaggtc ggcaggacca tggcgctgtc gccggagtgg atgccggcct cctcgatgtg
     1741 ctccatcacc ccggccacca ccacctggcg ccggtcgcag atggcgtcga cgtcgacctc
     1801 gatcgcgtcc tccaagaact tgtcgatcag gaccgggtgc tccggggcca cttcgaccgc
     1861 cttggccatg aactcggtca gggagccggt atcgtaaact atctccatgg cccggccgcc
     1921 caggacgtag gaggggcgaa ccaggaccgg gtagccgatg gattcggcga tcttccgggc
     1981 cgcctcgaag gaggaggcgg tcccgttggg cgcctgctgg aggcgcagtg aattgagcag
     2041 ccggctgaag cgcttccggt cctcggcccg gtcgatgctg tccggcgagg tgcccaggat
     2101 cttgaggccg gaccgctgga gcgggatggc caggttgagc ggggtctggc cgccgaactg
     2161 gacgatggcc cccagcggcc gctcgcgttc gacgatgttg agcacgtcct cgagggtgag
     2221 cggctcgaag tagaggcggt cggaggtgtc gtagtcggtg gagacggtct ccgggttgca
     2281 gttgaccatg atgctctggt agccggcctc gcgcagggcg aaggaggcgt gcacgcagca
     2341 gtagtcgaac tcgatgccct ggccgatccg gttggggccg ccgcccagga tggccacctt
     2401 ccgttttcgg tcggcccggc actcgtcgcc gcggtcgtaa gaggagtaga aataaggacg
     2461 ggccacctcg atttcgctgg cgcaggtgtc gaccagtttg aagaccggct cgatgcccag
     2521 tttcttgcgc cgggagcgga cctgaacctc ggtcaggccg ctcaggcagg ccagctggcg
     2581 gtccgagaag ccgtactcct tggcccggcg gagggatcgg cggtcgagcc gcttgaagcc
     2641 ggcctccgcc tcgaccagtt cgcggatctg cccgatgaac caggggtcga tccgggacag
     2701 gcggtagatt tcattggtgc tgaagccctg ccggatggcg tggcgcaggt agaagaggcg
     2761 ctcgccgttt ggcacccgca gccgctcggc caccgtttcc cggtccggct cgggaccgtc
     2821 cttgccgtcg gcgcccaggc cgaaacggcc ggtctccagg gcccgcaacc ccttctggag
     2881 cgactccttg aaggtgcggc cgatcgacat gacttcgccc accgatttca tcgaggtgtt
     2941 gagggtcggg tcggcgccgg cgaacttttc gaaggtgaag cgggcgatct tgaagacgca
     3001 gtagtcgacg gtcggctcgt agaaggccga ggtccggccg gtgatctcgt tgcggacctc
     3061 gtccagggtg aagccgacgg ccagcttggt ggcgatgcgg gcgatcggga agccggtcgc
     3121 cttggaggcc agggccgagc tgcgcgagag gcgcgggttg acctcgatga tcacgatccg
     3181 gccgttttcc gggttgaggc cgaactggat gttggcgccg ccgccgttga tgccgatcgc
     3241 ccggatgatg cgccggcaga gttcggcgaa gcggccgtac tcgccgctgg tcagggtctg
     3301 agccggggcc accacgatgc tgtcgccggt atggacaccc atcgggtcga cgttctccat
     3361 cgaggtgacg atgatggtgt tgtcggcgca gtcgcgcatc acctcgaact cgatctcctt
     3421 ccagcccagg accgactcct ccaccagcac ctcgccgatc gggctggcct cgatgccctt
     3481 ggccagggcg gccttgagtt cttccaggtt gtagacggtg gaaccgccgg tgccgcccag
     3541 ggtgtaggcg ggacggagaa tgagcgggaa gccgatcttc tcggcgatgg cgatcccgtc
     3601 ctcgaccgag taggccaacc cgctggccgg gacctcgacg ccgatcgcct gcatcgtctt
     3661 cttgaagagg tcgcgggttt cggcccgggc gatcgcctcg gcgtcggcgc cgatcatctc
     3721 gactccgtac ttttccagca cgccctcccg ggccaggaag gtggccaggt tgaggccgac
     3781 ctggccgccc agggtgggca gcagggcatc cggcctctcc ttctcgatga cccgggtcag
     3841 gaactcgatc gaaagcggct cgatgtaggt ggcgtcggcc aggccggggt cggtcatgat
     3901 ggtggccggg ttggagttca ccagcaccac ccggtagccc tcctccttga gcgccttgca
     3961 ggcctgggaa cccgaatagt caaactcgca ggcctggccg atgatgatcg gccccgagcc
     4021 gatcaggaga atcttcttca ggtcggttcg tttaggcatt gaggaacccc gcgaaggttt
     4081 cgaagatcgg gtcgtcgttg gccgggtagt actggaggcc ggccagcggc cgttcccggt
     4141 ggcgcacgcc ttccaccgtc cggtcgttca ggttgaggtg ggttacctgg agcccgggtc
     4201 cgagcgaagc cgggtcgatg gccagggagt ggcactggct ggtggagagg caccggcggc
     4261 cggccaggtc ctggaccggc tggttcaggc cgtagtggcc ggtcttcatc cggaaggcgg
     4321 cggcgcccag ggccaggcat aggacctgga agccgaggcc gaaggccagg acgggcagtt
     4381 tttcggagag ggtccggacc gcggccacgc tttcggacag cccggccggg tcgcccgggc
     4441 cgctggagag gaagaacgcg tccggcttca gttccaggac cgcctcggcc ggggtgtcaa
     4501 aggggaccag ggtcacccgg ccgcctaagg cggccagctg gcggagggaa tcggcccggg
     4561 ccccgaaatc gtaagccacg aa
//
LOCUS       DAOQYN010000160         4569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_19269_length_4569_cov_4.859770, whole genome shotgun sequence.
ACCESSION   DAOQYN010000160 DAOQYN010000000
VERSION     DAOQYN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19269_length_4569_cov_4.859770"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PKN80_06745"
     CDS             complement(<1..1441)
                     /locus_tag="PKN80_06745"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HNQ35747.1"
                     /translation="MNWKRNWNLAKLACLLLPLFPGLARAESGLGDWETVGPGAALAV
                     SAPDPSGAGSAGVEVRLDSGREAWIRQVRTLDWGSFGRLSFTVSVPAKAPGPVTFICY
                     LKDRDWNWFQSSLFSLAAGQSRRITLDLSSESVDWQGYAHLKSWDAILRRQLQEIGFK
                     FYWPAAGTGRVILSGIEPETAAAPAELCLYNFRENTRRLELYARFETAFEIPLFFDNP
                     FDPAEVDIQGVFSAPSGRQVSVPAFIYQDFVRHLDQEEGERLAPYGKTEWRLRFTPQE
                     TGRYTYRITLKTAGRSQEFPGGEFEVTPSNRPGFLRWDKQDPRWLALDNGEFFYPIGH
                     TLRSPDDQREAYRYEFKQTKGWGTFLYDDYFREMARHDENYVRLWMSAWWAGLEWTPA
                     YAPHYDGLGRYSLENAWRQDLVLERAEETGIYAILTLINHGQYSAGSDREWWDNPHNV
                     VNGGFLKSPEEFFTDPLAREYFKRRLRYLV"
     gene            complement(1410..3797)
                     /locus_tag="PKN80_06750"
     CDS             complement(1410..3797)
                     /locus_tag="PKN80_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35748.1"
                     /translation="MFKSQAFHFRIAGLFCLALFLSASILKAQEKPPLLTEAQALIQK
                     KDYAGAVERLERLVKLVPDDPETRYYLATALLAQKQAETARPHLEFYLEKGPVAPFPR
                     FNETYRNLVSFYRDKKLPEEVIRVSHLFRNRVPPGDRNIQYSLDFAAGNESSARRELG
                     EKAFSTKLYQVALEHFLAAEQLGGNKRFLWDRIARCYRALARLPEARKAYLLAIRLAD
                     KDWSYRFNTIVGLAGILVSTGDWEAAAKELADDPDGSEALKIARLAISGRAAAAFEEA
                     VGVDSRFNQKGELVGYLVRSLVSDDLVPDTGKEGMLYAYLKRYPTGNLASWSVNNLQR
                     FYRQAPGRENAGREAVTRLINDILAAHPGNPEVQKFARRVVDELWWEWGRRPGGQRDD
                     RRLRADLYARIRQACPDGPVVADLLRREARMRLDDGDYRTAGDLYRELVERYNDRNSI
                     PQLVEAVALAGDPARAAGLLKDFLDKQPAPEERYQVKLGQYYLMAGLVDQGLAWLTRV
                     RERTSRDEIRNQVYNLVKDYREVIPGQEPLPDTGLIVLFLESASRRSYFTNSVSLEKG
                     SPLIFQQVEELQVYPFAAGTGECQFGLDFTASKRPVLAEPYAIIRTEGENFTGHWPGQ
                     AAAGPHRWREPAAVYRLLFPWQEVPVPAVEARRSYSRVGTDTMLVEIEVRVPGPGWRV
                     TLEWNRRVGRLQNITPQPDVSEGDRLVYESVPNVFRLKLEGRRAGNPDFYYPRLLVGR
                     YESETRTPGTRGRDLDFRLPSGTGYRLESGRDIVFRRGLIHSETVYELEEKLEPR"
     gene            complement(3832..4392)
                     /locus_tag="PKN80_06755"
     CDS             complement(3832..4392)
                     /locus_tag="PKN80_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNQ35749.1"
                     /translation="MKSKRLPGRFFERVFHPGLLFLREYYHLWTVSPGLVYRACQYRR
                     RRLAEVIRALGLRSVLNLRGANPRARWWRDEVEICARLGVGHYDLNLSARQVPSPKQL
                     EELVRILASAPKPLLFHCEGGGSRTGLASAIYKYRFEGLSCRRARRQFSVIYGHFPLF
                     HPSTRAMERAFLDFCRISGSGRPLPE"
BASE COUNT          870 a         1449 c         1475 g          775 t
ORIGIN      
        1 ccacgagata ccggagccgg cgcttgaagt actcccgggc gagcggatcg gtgaagaatt
       61 cctcgggtga tttcagaaaa ccaccgttga ccacgttgtg cgggttgtcc caccactccc
      121 ggtcggaacc ggcgctgtac tggccgtggt tgatcagggt caggatggcg taaatgccgg
      181 tctcctcggc ccgctcaagc accaggtcct ggcgccaggc gttctccagg ctgtagcgcc
      241 ccagcccgtc gtaatgcggg gcgtaggcgg gcgtccattc caggccggcc caccaggccg
      301 acatccagag gcggacgtaa ttttcgtcgt ggcgggccat ctcccggaag taatcgtcat
      361 agaggaaggt gccccagccc ttggtctgct tgaactcgta ccggtaggcc tcacgctggt
      421 cgtccggaga gcgcagggtg tggcctatcg ggtagaagaa ctcgccgtta tccagggcca
      481 gccagcgcgg gtcctgcttg tcccagcgca ggaagccggg ccggttcgac ggggtgacct
      541 cgaactcccc gcccgggaac tcctggctgc ggccggcggt tttaagggtg atccgatagg
      601 tgtagcggcc ggtctcctgc ggggtgaaac ggaggcgcca ctcggtcttt ccgtaagggg
      661 ccagccgttc gccctcctcc tggtcgaggt ggcgcacgaa atcctggtag atgaaagccg
      721 gcacgcttac ctggcgtccg gagggcgcgc tgaaaacgcc ctggatgtca acctcggccg
      781 gatcaaacgg gttgtcgaag aagagcggga tctcgaaggc cgtctcgaag cgggcgtaaa
      841 gttccaggcg cctggtgttt tcccggaagt tatagaggca gagctccgcc ggcgcggcgg
      901 cggtttccgg ctcaatcccg gaaaggatga cccggcccgt cccggcggcc ggccagtaga
      961 acttgaagcc gatctcctgg agctggcgtc gcaggatggc atcccagctc ttgaggtggg
     1021 cataaccctg ccagtcaacg ctttcgctgg aaagatcgag ggtgatccgc cgcgactggc
     1081 cggcggccag actgaaaagc gaggactgga accagttcca gtcgcggtcc ttcaggtaac
     1141 agataaaggt gaccggaccg ggggcctttg ccggcacgct cacggtgaag gagagacggc
     1201 cgaagctgcc ccaatcgaga gtgcgcacct ggcggatcca ggcctcccgg ccggaatcaa
     1261 gccttacctc aacgccggcc gacccggcgc cggaaggatc gggcgcggaa accgccagcg
     1321 cggcgccggg gccaacggtc tcccagtcgc cgaggccgga ttcggcccgg gccagccccg
     1381 ggaacaatgg cagcaacaga caggctaact tagcgaggtt ccagtttctc ttccagttca
     1441 tagacggttt cggaatgaat caggccgcgc cggaaaacga tatcccggcc gctttccagg
     1501 cggtacccgg taccggaggg aagccggaaa tccaggtccc ggccgcgcgt ccccggagtc
     1561 cgggtctctg actcgtaacg ccccaccagc aggcggggat aataaaaatc ggggttgccg
     1621 gcgcgacgcc cctccagttt taaccgaaag acgttcggga ctgactcgta gaccagccgg
     1681 tcgccctcgc tgacatcggg ttggggcgtg atgttctgaa gccggccgac ccggcggttc
     1741 cattccagcg taacccgcca gcccggaccg ggaacccgga cctcgatctc gaccagcatg
     1801 gtgtcggttc ccacccggct gtaagaacgc ctggcctcca ccgccggcac cgggacctcc
     1861 tgccagggaa agagcaggcg gtagaccgcc gccggttcgc gccagcggtg cggaccggcc
     1921 gcggcctggc ccggccagtg gccggtgaaa ttttctccct cggtgcggat gatggcgtac
     1981 ggttcggcca ggaccggacg tttcgaagcc gtgaagtcga gaccgaactg gcactcgccg
     2041 gtcccggcgg cgaagggata tacctgcaat tcctccacct gctgaaagat cagcggactt
     2101 cccttttcga gcgaaacgga gttggtgaag tacgagcggc ggctggccga ctccaggaag
     2161 agaacgatca gtccggtgtc gggcaacggt tcctggccgg ggatcacctc ccggtaatcc
     2221 ttgacaaggt tatagacctg atttcgaatc tcgtcccggc tggtccgctc cctgaccctg
     2281 gtaagccagg ccagcccctg atcaaccaac ccggccatca ggtaatactg ccccagtttc
     2341 acctggtacc gttcttcggg cgccggctgc ttgtccagga agtccttcag caatccagcg
     2401 gcccgggccg gatcgccggc cagggccacg gcctcaacca gctggggaat cgagttccgg
     2461 tcgttgtagc gttcgaccag ttcgcggtag aggtcgccgg ccgtccggta gtcgccgtcg
     2521 tcaaggcgca tccgggcctc ccggcgaagc aggtcggcca ccaccggccc gtccgggcaa
     2581 gcctggcgta tccgggcgta aagatcggcc cggaggcgac ggtcatcccg ctgaccgccc
     2641 ggccgccgcc cccattccca ccagagctcg tccaccaccc gccgggcgaa cttctgaacc
     2701 tccgggttcc cgggatgggc cgcaagtata tcgttgatca agcgagtgac cgcttcccgg
     2761 ccggcattct cccgaccggg cgcctgccga taaaaacgct gcaggttgtt gaccgaccag
     2821 gaggccaggt ttccggtcgg gtaacgcttc aggtaggcat aaagcatccc ttccttaccg
     2881 gtatccggga ccaggtcgtc ggagaccagg ctccggacca ggtatccgac cagttcgccc
     2941 ttctggttaa accggctgtc aaccccgacc gcctcttcga aggccgcggc ggcccggccg
     3001 ctgatcgcca ggcgcgctat cttcaaggcc tccgatccgt ccggatcgtc cgccagctcc
     3061 ttcgcggcgg cctcccagtc gccggtcgaa accaggatcc cggccagccc gacaatcgtg
     3121 ttgaaacggt acgaccagtc cttatcggcc agccggatgg ccagcaggta ggccttacgg
     3181 gcttcgggta aacgggccag tgcccggtaa cagcgagcga tccggtccca gagaaaacgc
     3241 ttgttgcccc ccagctgttc agccgccagg aagtgttcca gggcaacctg gtaaagtttc
     3301 gtcgagaagg ccttctcgcc caattcccgc cgggcgctgg attcgttgcc ggcggcgaaa
     3361 tcgagtgagt actggatgtt gcggtctccc ggcggcaccc ggttgcggaa gagatggctg
     3421 acccggatca cctcctcggg cagcttcttg tcgcggtaga acgagacgag gttgcgatag
     3481 gtttcattga accggggaaa cggggccacc ggtccctttt caaggtagaa ttcaaggtgg
     3541 ggccgggccg tttcggcctg cttctgggcc agcagcgccg tggccaggta ataacgggtc
     3601 tcgggatcgt cgggaacaag tttgaccagg cgctcgagcc gttcgaccgc cccggcgtaa
     3661 tcctttttct ggataagcgc ctgggcctcg gtaagcaggg gcggcttctc ctgggccttg
     3721 aggatcgaag cgctcaaaaa cagggccagg cagaacaaac ccgcgatccg gaaatgaaag
     3781 gcctgcgatt taaacatatc tactttagtt gccggcgggg gcggaacccg tttactcagg
     3841 gagcggccgg ccggacccgg atatccggca gaaatcgaga aaagcccttt ccatcgccct
     3901 ggtggagggg tggaaaagcg gaaagtgccc gtatatcacc gaaaactgcc ggcgggcgcg
     3961 ccggcaggaa agaccctcga aacggtactt gtaaatcgcg gaggccaacc cggtacggct
     4021 gccgccgccc tcacagtgaa acaggagggg cttgggcgcg ctggccagta tccggaccaa
     4081 ctcttccagc tgcttcgggc tgggcacctg ccgggccgac aggttaaggt cataatggcc
     4141 gacgcccagc cgggcgcaga tttcaacttc atcccgccac cagcgcgccc gcggattggc
     4201 gccgcgcagg ttcagaacgc tcctcaaacc gagggcccgg ataacttcgg ccaggcgccg
     4261 ccttcggtac tggcaggcgc ggtaaaccaa cccgggactg acggtccaga gatggtaata
     4321 ttcacgcagg aaaagcaagc cgggatgaaa gacacgctca aaaaaacggc ctgggagtcg
     4381 ttttgatttc attccaaacc cggcgccggc gtaaagggtg ggggaaggca gtttatccta
     4441 taaaatttaa agagcaaaaa cgatcaagag cgggaaacgg gattcggcgg cctcgggtcc
     4501 agcttcggct cgggcgctgc gccgccgggg aaaggtacgc ttaaatccgg cgcgcggcta
     4561 actcgccgt
//
LOCUS       DAOQYN010000161         4568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_19275_length_4568_cov_3.650565, whole genome shotgun sequence.
ACCESSION   DAOQYN010000161 DAOQYN010000000
VERSION     DAOQYN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19275_length_4568_cov_3.650565"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="PKN80_06760"
     CDS             complement(<1..1529)
                     /locus_tag="PKN80_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNQ35750.1"
                     /translation="MDKKYILVKDTRRAGADQTEHFAGLLAAKIQAGPAADSVQVVRA
                     ADIGFYDQGLVLKFLPAGPVYLNVSEADLDRVVSETVLGGRVLEDLAAAGTARQLRVV
                     LRNCGVINPESLEEYIRRDGYRALSQALFEYTPEKVIEEFKKSGLRGRGGAGYPTWLK
                     WHLTRSVPGDEKYVICNADEGDPGAYMDRSILEGDPHSVLEGMIIAGYAIGAGRGYFY
                     IRAEYPLAIERIGKAIGQARERGLLGAGILGSNFNFEVDIRLGAGAFVCGEETALIAS
                     IEGKRGTPRPRPPYPSVSGLWGKPTSINNVETFANVPVVMTRGGDWFAGIGTEKSRGT
                     KVFAVTGKVKNSGLVEVPMGVTLRQIVFDIGGGVRSGKKIKAVQTGGPSGGVIPESRL
                     DTPVDYENLQKLGSIMGSGGMIVMDEDDCMVDIAKFYLGFCVEESCGKCSPCRIGTYQ
                     LLKILERISEGEGRNEDLEKMRLIAQAMQKASLCGLGQTASNPVLSTLNYFETEYREH
                     I"
     gene            complement(1545..1895)
                     /locus_tag="PKN80_06765"
     CDS             complement(1545..1895)
                     /locus_tag="PKN80_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNQ35751.1"
                     /translation="MKKWNKSDLEKIRASQEPDRDWIRVGMSTCGIAAGAEAVFEYLR
                     EEVARRGLPIEVKKCGCLGMCYAEPLVEVRAAGLPRVIYGQVNRELARLIVESHLVEK
                     KLVSDHIYSFNREE"
     gene            complement(1902..2387)
                     /locus_tag="PKN80_06770"
     CDS             complement(1902..2387)
                     /locus_tag="PKN80_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNQ35752.1"
                     /translation="MSEGNDYHQELSGLLAKWKDKPGNLIMILHEIQNHYGFVPRQLS
                     LQLSALLDVPLARIYEVITFYNYFKLEAPGKHKISVCMGTACYLNGAPEILRELKSIL
                     GVEEGHTTKNGLFQLEVVRCLGCCGLAPVIMIDGKVYDKVKKNDIIDIISQFTRSKEP
                     E"
     gene            complement(2553..3158)
                     /locus_tag="PKN80_06775"
     CDS             complement(2553..3158)
                     /locus_tag="PKN80_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNQ35753.1"
                     /translation="MSDPTVVYILLLLGILGLLAEFSTPGIGFPGIVGSICLFLAFYG
                     ISVLPINIAGLALIFVAAGFFILEAYTPSFGVLGIGGIVALIFGSLLLVKPFSYVRIY
                     YPAYLAAGLVFTGLVLLIATFAFRVYRKQVTTGKEGLVDEIGEARTDLDPKGTIYVHG
                     EYWTARSESGPVRKGEAVRVVGMEGHILRVVKREEPPSDPA"
     gene            complement(3162..3860)
                     /locus_tag="PKN80_06780"
     CDS             complement(3162..3860)
                     /locus_tag="PKN80_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35754.1"
                     /translation="MRRLVNFRGILLFAFLLLVSARSAPAAEVVVLPLKGAVNPAMVE
                     LVQDVLAAAERDQAGLVLLEIDTPGGLLSSTRDIVEAIFQSRVPVAAYVAPSGAQCAS
                     AGTFIALACPVLAMAPGTNLGAAHPVMPFGKMDETMEKKAVNDAAAWIGSLAGRWGRN
                     RIWAESAVRESAALDENGALSEGVIDLVAADRNDLLVKIDGRRVTLEDKAVVIRTTGA
                     PVREIKPNLRQRVL"
     gene            complement(3868..>4568)
                     /locus_tag="PKN80_06785"
     CDS             complement(3868..>4568)
                     /locus_tag="PKN80_06785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNQ35755.1"
                     /translation="LTASELDRYRGPGAEVALALTRTARAVEPGWTETAAAARMAGEL
                     MAGGIFPTVLLVAADERIGLYRHPLPTGRRVGKCLMLVACGRRQGLIVSITRLLHFGP
                     LPAELARRHAAVARVDGALIAATRPGRPLGEVFRAGRTAYERSGFPEEWRLHHQGGPT
                     GYLGRYFRVTEAETRPAAERAAFSWNPSIAGTKSEDTIIIDGEKLPEVVSADPAWPVL
                     EVETGPARPDILIR"
BASE COUNT          769 a         1578 c         1442 g          779 t
ORIGIN      
        1 tggatgtgtt cgcggtactc ggtctcgaaa taattgaggg tggagagcac cgggttggac
       61 gccgtttggc ccaggccgca gagcgaggcc ttctgcatcg cctgcgcgat gagccgcatc
      121 ttttccaggt cctcgttgcg gccctcgccc tcggagatcc gctccagtat cttgagcagc
      181 tggtaggttc cgatccggca gggcgagcac ttgccgcagg actcctcgac gcagaagccg
      241 aggtagaact tggcgatgtc caccatgcag tcgtcctcgt ccatcacgat catgccgccc
      301 gagcccatga tcgaaccgag tttctgcagg ttctcgtaat cgaccggcgt atccagccgg
      361 ctctccggga tgacgccgcc agagggtccg ccggtctgga ccgccttgat cttcttgccg
      421 gagcgcacgc cgccgccgat gtcgaagacg atctggcgca gggtgacgcc catcggcacc
      481 tcgaccaggc cggaattctt gaccttgccg gtgacggcga aaaccttggt cccccgcgac
      541 ttctcggtgc cgatgccggc aaaccagtct ccgccccggg tcatgacgac cggcacgttg
      601 gcgaaggtct cgacattgtt gatcgaggtc ggcttgcccc agagacccga gaccgagggg
      661 tagggcggcc gcggccgcgg ggtaccccgc ttgccctcga tggaggcgat cagggccgtc
      721 tcctcgccgc agacaaaggc cccggcgccc agccgtatgt cgacctcaaa attgaaattg
      781 ctgcccagga tgccggcgcc cagcagtccc cgttcgcggg cctgaccgat cgccttgccg
      841 atgcgctcga tggccagcgg gtactcggcc cggatgtaaa aataaccgcg gccggccccg
      901 atggcgtagc cggcgatgat catgccctcc agcaccgagt ggggatctcc ctccaggata
      961 ctgcggtcca tgtaggcgcc cgggtcgccc tcgtcggcgt tgcagatgac gtacttctcg
     1021 tcgccgggga cgctgcgggt gaggtgccac ttcagccagg tcgggtagcc ggcgccgccc
     1081 cggccgcgca ggccgctctt cttgaactcc tcgatcacct tctccggggt gtactcgaag
     1141 agggcctggc tcagggcccg gtacccgtcc ctccggatgt actcctcgag gctctccggg
     1201 ttgatcaccc cgcagttgcg caggaccacc cgcagctgcc gggccgtccc ggccgccgcc
     1261 aggtcctcca ggacccggcc gccgaggacc gtctcgctga ccacccggtc caggtcggcc
     1321 tccgaaacgt taaggtagac cgggccggcc ggcaggaact tgagcaccag cccctggtcg
     1381 taaaaaccga tatcggccgc ccgcaccacc tggaccgagt cggccgccgg gccggcctgg
     1441 atcttcgcgg ccagcagccc ggcgaaatgc tcggtctggt cggccccggc gcgccgggtg
     1501 tccttgacga gtatgtattt cttgtccata aaagtagtcc ggtcttactc ttcgcggtta
     1561 aacgagtaga tatgatcgct caccagcttc ttctccacca ggtggctctc cacgataagc
     1621 cgggccagtt cccggttgac ctgcccgtag atcacccggg gcagcccggc cgcccgcacc
     1681 tcgaccagcg gctcggcgta gcacatcccc aggcagccgc acttcttgac ctcgatcggc
     1741 aggccgcgcc tggcgacctc ctcgcgcagg tactcgaaga ccgcctcggc gccggcggcg
     1801 atgccgcagg tgctcatgcc gacccgtatc caatcccggt ccggttcctg cgaggcgcgg
     1861 atcttttcca ggtccgactt gttccacttc ttcatgtttc cttactccgg ctccttgctc
     1921 cgggtgaact gtgaaatgat gtcgatgatg tcgttcttct tcaccttgtc gtaaaccttg
     1981 ccgtcgatca tgatcaccgg ggccagcccg caacagccca ggcagcggac cacctccagc
     2041 tggaagaggc cgttcttggt cgtgtgcccc tcctcgacgc ccaggatact cttcagctcc
     2101 cgcaggatct ccggggcccc gttcagatag caggcggtgc ccatgcagac cgagatcttg
     2161 tgcttgccgg gggcctccag cttgaaataa ttataaaagg tgatcacctc gtagatcctg
     2221 gccagcggca cgtccagcaa ggccgaaagc tgcagggaaa gctgccgggg cacgaacccg
     2281 taatggttct ggatctcgtg gaggatcatg atcaggttgc ccggcttgtc cttccacttg
     2341 gccagcaggc cgctcaattc ctggtgataa tcgttccctt cgctcatgcc ggactcccgc
     2401 gctgtttggg gtggaatggt caaaagattg tgtttatttt cacaaagctt tcggaaaaga
     2461 ttataacatt atcctgtcgc acttggcaag cttaagccat tatagcacaa atgccgtttt
     2521 ttgccaatag ttaatgcctg agttcacaat ggtcaggcgg ggtcggacgg gggctcctcc
     2581 cgcttcacca ccctcaggat gtgcccctcc atgccgacca cccgcaccgc ctcccccttg
     2641 cgcaccgggc cgctctccga gcgggccgtc cagtactcgc cgtgaacgta aatggtgccc
     2701 ttcgggtcca ggtcggtccg cgcctcgccg atctcgtcca ccagcccttc cttgccggtg
     2761 gtcacctgct tccggtagac ccggaaggcg aaggtggcga tcagcaggac caggccggtg
     2821 aagaccaggc cggcggccag gtaggccggg tagtaaatcc gcacgtacga gaagggcttc
     2881 accagcagca gcgaaccgaa aatcagggcc acgattcctc cgattcccag caccccgaac
     2941 gacggggtgt aggcttccag gatgaagaag ccggccgcca cgaagatcag ggccaggccg
     3001 gcgatgttga tcggcagcac cgagattccg taaaaagcca ggaagaggca gatcgacccc
     3061 acgatccccg ggaaaccgat gccgggggtg gagaactcgg ccagcagtcc caggatgccc
     3121 agcaggagca ggatgtaaac cacggtgggg tcgctcaccg actagagcac ccgctggcgc
     3181 aggttgggct tgatctcgcg caccggggcg ccggtggtgc ggatgacgac ggccttatcc
     3241 tccagggtca cccggcgccc gtcgatcttg accagcaggt cgttgcgatc ggcggccacc
     3301 aggtcgatca cgccctcgct cagcgcgccg ttctcgtcca gcgccgcgct ctcgcgcacc
     3361 gccgactcgg cccagatccg gttgcgtccc cagcgtccgg ccagcgaccc gatccaggcg
     3421 gcggcgtcgt tgacggcctt cttctccatc gtctcgtcca tctttccaaa aggcatgacc
     3481 gggtgcgcgg cgcccaggtt ggtgccgggc gccatggcca gcaccgggca ggccagggcg
     3541 atgaaagtcc cggccgaggc gcactgggcg ccggacgggg ccacgtaggc ggccaccggc
     3601 acccggctct ggaagatggc ctcgacgata tcgcgcgtcg agctgagcag gccgcccggg
     3661 gtgtctatct ccaggagcac caggccggcc tggtcccttt cggccgcggc caggacatcc
     3721 tggaccagct cgaccatggc cgggttgacc gcgcccttca ggggcaggac caccacctcg
     3781 gccgccgggg cagaacgggc tgaaaccaac agcagaaagg cgaacagcag gattccacga
     3841 aagttaacca atctgcgcat gggccaccta cctgatcagg atgtccggcc gggccggccc
     3901 cgtctcgacc tccagaacgg gccaggccgg atcggcggaa acgacttccg gtaacttctc
     3961 cccatctatg ataatggtat cctcgctctt ggtgccggca atcgacgggt tccaggagaa
     4021 ggcggcccgt tcggccgccg gccgcgtctc ggcctcggtc acccggaaat accggcccag
     4081 gtacccggtc gggccgccct ggtgatgcag gcgccattcc tccgggaagc cgctccgctc
     4141 gtaagcggtc cggccggccc ggaagacctc gcccagcggc cggcccggcc tggtggcggc
     4201 gatcagggcc ccgtccaccc gggccaccgc cgcgtggcgg cgcgccagct cggcgggcag
     4261 gggcccgaag tgcaggagcc gggtgattga aacgatcagc ccctggcgcc ggccgcaggc
     4321 gaccagcatc aggcacttcc cgacccggcg gccggtggga agcgggtggc ggtaaaggcc
     4381 gatgcgctcg tcggcggcca ccagcaggac ggtcggaaag atgccgccgg ccatcagctc
     4441 gccggccatc cgggccgcgg ccgccgtctc ggtccagccg ggctccaccg cccgggcggt
     4501 ccgggtcagc gccagggcca cctcggcgcc ggggccccgg taacggtcca gctcggaagc
     4561 agtcaggg
//
LOCUS       DAOQYN010000162         4483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_19759_length_4483_cov_4.254291, whole genome shotgun sequence.
ACCESSION   DAOQYN010000162 DAOQYN010000000
VERSION     DAOQYN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19759_length_4483_cov_4.254291"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..693
                     /locus_tag="PKN80_06790"
     CDS             142..693
                     /locus_tag="PKN80_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNQ35756.1"
                     /translation="MENGKDELRLTFEELVNQEGKKVYNLAYRLCGNREEARDLAQET
                     FARACENFGRFRGRSRVFTYLYRITYNLWCNRIRHRSRHPSFSLTPDDPERRPWEPAD
                     PAPAPDRALEAEERLELARRCLEQLEPADRLIIVLREVEDRSYEEIAAVLNCRLGTVK
                     SRLARARESLREKASPYLEAWLK"
     gene            690..1937
                     /locus_tag="PKN80_06795"
     CDS             690..1937
                     /locus_tag="PKN80_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNQ35757.1"
                     /translation="MKKNERHATPADFSAYLDGRLAGAGREGFESHLAGCGECGRRLK
                     ELELLRGLMRELPAADPGPGFERAVLERARDRGRRYAFAAWYRRTAYGLAGLGLILIV
                     AIYLQRPAPLMETGPERLLEVQPSEAPAPLPEAGPPVRAAKPAGEEKKSAGRFRNESG
                     AESLRAQGGGSAVKLDDQARSYEAAPESVERKAPAVGSASTLSKGELAARPPAPMMSA
                     PAAAMNEESGRGTADRPAPGLEAPAPAVSVARESAGPVAGSALDVAGRRSSISTAGAL
                     EKEAEVRGLEGASRSDTLTAATGSDSGPRLARLPAAPANLLIRNSRDWSRLWQTQNTV
                     QNLSLPLPPVDFRKQMVVAVPTRQENRAYQVLRSEEKADRVIIYYRDVTTEKCDKDEA
                     PPEPPYQVQVVNVRPRVEFQKAP"
     gene            2026..3603
                     /locus_tag="PKN80_06800"
     CDS             2026..3603
                     /locus_tag="PKN80_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNQ35758.1"
                     /translation="MRAELRDSLEFLFPDSPVGEKPVSRLKLDLARGATASVHLLLNG
                     LKTGAGLRCRVRSGGRPVAGAGWFRLVEVPVEVNTGLEAFVEKEGEPNPHVIRRAPFK
                     VYDAMEPVAGSLRVAGPAMALRLQIPVDRQEKPGCRRYEIEVEVAGERQSFILETVVH
                     RPAIPPSGKDSFPYTNWFWCEPIARNHRLEPWSPAHWRILGRYAKLMARARQNTFLLS
                     LGSVFEFQKDRPVLNRPRLRRLVKLFTEAGLYYIEGGHLATRHKGEWAATYFDLVLKP
                     VAATSPEGNALLASIGRQLWSEIEGQGWGNRWIQHVADEPTAGLAADYRILCGMARKY
                     LPGLPLLDATMDPALAGSVDIWCPLNRSYQKHRERFVEYQLLGDLVWFYTCCAPGGPW
                     LNRLLDMELLRPALLGWGAAHFGLDGFLHWGLNYLQPGRDPFRTSVMAPITPKSWPAG
                     DTHVVYPGPDGPWSSLRLEAQREGFEDYELLRRLRDRNPRRLAAVIRPVFRAFDDYTR
                     DTAVFRRGRRALLAALE"
     gene            3702..>4483
                     /locus_tag="PKN80_06805"
     CDS             3702..>4483
                     /locus_tag="PKN80_06805"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNQ35759.1"
                     /translation="MDNPRKRGHRSGFSLTGRGGLRLAPVILLALAALAAAVPALADG
                     LIVIPRPIPPGPFPPPIPRPIPPEPPFPLEVRYHRVEVTISDQAAVTRIDQEFFNPGS
                     SRLEGIYLFPLPEGAVIRDFALEIDGKPAAAELLDAEKARRIYEDIVRRSRDPALLEY
                     SGQGAFRVRVFPIEPRSPKRIKLSYAQPLRSESGLVGYTYPLNTEKFSARPLEEVVVR
                     VSIKAAGRIKSVYSPSHPVEVRRRGDHQAEAVFEARQVRPDK"
BASE COUNT          684 a         1557 c         1550 g          692 t
ORIGIN      
        1 atcctgccgg cggccctccg cccctacctg gataacctgg aagccatcac tccctcggaa
       61 taagggcggc cggagaccgg ccgacccggc gttgccgcgg aacctttttc ccttttcggt
      121 gtccaattat ctgccagcag gatggagaac ggaaaagatg agcttcggtt gaccttcgag
      181 gaactggtca accaggaggg gaaaaaggtg tacaacctgg cctaccggct ctgcggaaat
      241 cgcgaggagg cgcgggacct ggcccaggag acctttgccc gggcctgcga gaacttcggc
      301 cgcttccgcg gccgcagccg ggtcttcacc tacctctacc ggatcaccta taacctctgg
      361 tgcaaccgga tccgccatcg aagccgtcat ccttccttct ccctgacgcc ggacgacccg
      421 gaacggcggc cctgggaacc ggccgacccg gccccggcgc cggaccgggc cctggaagcc
      481 gaggagcggc tggagctggc ccggcgctgc ctggagcagc tggagccggc cgaccgcctg
      541 atcatcgtgc tgcgcgaggt ggaggaccgc tcctacgagg agatcgccgc ggtgctcaac
      601 tgccggctgg gcacggtcaa atcgcgcctg gcccgcgccc gcgagagttt acgggagaag
      661 gcctccccat atcttgaggc ctggctgaaa tgaagaagaa cgaacgacac gcgaccccgg
      721 cggatttctc cgcctacctg gacggccggc tggccggcgc cggacgggag ggcttcgagt
      781 cccacctggc cggctgcggg gagtgcggcc gccgcctgaa ggagctggaa ctgctccggg
      841 ggctgatgcg ggagctgccg gcggctgacc cggggccggg ctttgaacgg gccgtcctgg
      901 aacgggcgcg ggaccggggg cggcggtatg ccttcgccgc ctggtaccgg cggacggctt
      961 acggcctggc cggactcggc ctgatactca tcgtggccat ctacctgcag cggccggcgc
     1021 cgctgatgga gacgggcccg gagcggttgc tcgaggtcca gccgtccgaa gcgccggcgc
     1081 cgcttccgga ggccgggccg ccggttcggg ccgcgaagcc ggccggggag gagaagaaat
     1141 cggccgggag attccggaat gaatccggcg cggagtctct ccgggcccaa ggcggcggtt
     1201 ccgccgttaa gctggatgat caggcccggt cttacgaggc ggcgccggaa tcggtcgaaa
     1261 ggaaagcgcc cgcggtcgga tccgcttcca ccctgtcgaa gggcgaactg gccgcccggc
     1321 cgccggcacc gatgatgtcg gcgccggccg ccgccatgaa tgaagaatcc ggacggggga
     1381 cggccgatcg gccggctccc ggcctcgaag ccccggcgcc ggcggtctcg gttgcccggg
     1441 agtcggccgg tccggtggcc gggagtgcgc ttgacgtggc cggccgccgt tcttccattt
     1501 cgactgccgg cgccctggaa aaggaggcgg aggtccgcgg cctggagggc gccagccgga
     1561 gcgataccct gacggccgcc accgggtccg acagcgggcc gcgcctggcc cggctgccgg
     1621 ccgcgcccgc caacctgctc atccgcaaca gccgcgactg gtcccgcctc tggcagaccc
     1681 agaatacggt ccagaacctc tccctgcccc tgccgccggt cgacttccgg aagcagatgg
     1741 tggtggccgt gcccacccgg caggagaacc gcgcctacca ggtcctgagg tccgaggaga
     1801 aggcggaccg ggtgatcatc tactaccggg acgtgacgac cgaaaagtgt gacaaggacg
     1861 aggcgccgcc ggagccgccc taccaggtcc aggtggtcaa cgtccgcccc cgggtcgagt
     1921 tccagaaggc gccctgaaaa agcgtcgccc tgcctttctt ccccgccgcc cgggtgctat
     1981 aatctccctg gaaacgcctg ccggttcacc caccaggagg tttttatgcg cgcggaactg
     2041 cgggacagcc tcgaattcct cttccccgat tcgccggtcg gcgaaaaacc ggtctccagg
     2101 ttgaagctcg acctggcccg gggcgccacg gccagcgtcc acctgctcct caacggcctg
     2161 aagaccggcg ccggcctccg ttgccgggtc cggagcggcg gccggccggt ggccggggcc
     2221 ggctggttcc ggctggtgga ggtgccggtg gaggtgaaca ccggcctgga ggccttcgtc
     2281 gagaaggagg gggagccgaa tccgcacgtc atccgccggg cgcccttcaa ggtttatgac
     2341 gccatggagc cggtggccgg gtcgctccgg gttgcggggc cggcgatggc cctgcggctg
     2401 cagataccgg ttgaccggca ggagaagccg gggtgccgcc gctacgagat cgaggttgaa
     2461 gtggccgggg agaggcagag ttttattttg gagaccgtgg tgcaccggcc ggccattcca
     2521 ccctccggca aagatagttt cccctatacc aactggttct ggtgcgagcc gatcgccaga
     2581 aaccaccggt tggaaccctg gagcccggcc cactggcgga tactcggccg ctacgcgaag
     2641 ctgatggcca gggcccgcca gaataccttc ctgctctcgc tgggttcggt cttcgaattc
     2701 cagaaggacc ggccggtcct gaaccggccg cggctgaggc gcctggtcaa gctcttcacc
     2761 gaggccggcc tctactacat cgagggcggc cacctggcca cccgccacaa gggggagtgg
     2821 gcggccacct attttgacct ggttctgaaa ccggtggcgg cgaccagccc ggagggcaac
     2881 gccctgctgg cctcgatcgg ccgccagctc tggtcggaga tcgaagggca ggggtggggg
     2941 aaccgctgga ttcagcatgt ggccgatgag ccgaccgctg gcctggcggc cgattaccgc
     3001 atcctctgcg gcatggcgcg caagtacctg cccggcctgc cgctgctgga tgccaccatg
     3061 gacccggccc tggccggctc ggtggacatc tggtgcccgc tcaaccgctc ctaccagaag
     3121 caccgggaac gcttcgtcga gtaccagctt ttgggtgacc tggtctggtt ctacacctgc
     3181 tgcgcgcccg gcggcccctg gctgaaccgg ctgctggaca tggagctgct gcggccggcc
     3241 ctgctgggct ggggcgcggc ccatttcgga ctcgacggtt tcctgcactg gggcctgaat
     3301 tatctccagc ccggccggga ccccttccgg accagcgtca tggcgcccat tacccccaag
     3361 tcctggccgg ccggcgacac ccacgtggtt tacccggggc cggacggacc ctggtccagc
     3421 ctgcgcctgg aggcccagcg cgagggcttc gaggattacg agttgctgcg ccgcctccgt
     3481 gaccgcaacc cgcggcggct ggccgccgtg atcaggccgg tcttccgggc cttcgacgac
     3541 tacacccgcg acacggccgt cttccgccgg gggcgccggg ccctgctggc cgccctggaa
     3601 tagacctcca gccctccgcc ggaacttttt cccggttccg gtgtctaatt ttccgcaacg
     3661 gccggaaacg gccgatcccg gttggttaga ggaggcggag catggataat ccacggaaac
     3721 gcggtcatcg aagcggtttc agtttgaccg gccggggcgg cttgaggctg gcgccggtaa
     3781 tcctgctggc gctggcggcg ctggcggcgg ccgtcccggc cctggccgac ggcctgatcg
     3841 tgataccccg gccgattccg ccggggccct tcccgccccc gataccccgg ccgatcccgc
     3901 cggagccgcc cttcccgctc gaggtccggt accaccgggt cgaggtgacc atctccgacc
     3961 aggcggcggt gacccggatt gaccaggagt tcttcaaccc gggctcaagc cggctggaag
     4021 gcatctatct ctttccgctg ccggagggcg cggtcatccg ggacttcgcc ctggagatcg
     4081 acggcaagcc ggcggcggcc gagctgcttg acgccgagaa ggcccgccgg atttacgagg
     4141 acatcgtccg ccgctcgcgc gacccggccc tgctggagta ctccggccag ggcgccttcc
     4201 gggtgcgcgt tttcccgatc gagccccgct cgcccaagcg catcaagctt tcctacgccc
     4261 agccgctccg gtccgagtcg ggcctggtcg gttacaccta cccgctgaat accgagaagt
     4321 tttcggcccg ccccctggaa gaggtggtgg taagggtaag catcaaggcg gccggccgca
     4381 tcaagagcgt ttactcgccc agccacccgg tcgaggtgcg ccgccgcggc gaccaccagg
     4441 ccgaggccgt cttcgaggcc cggcaggtcc ggccggacaa gga
//
LOCUS       DAOQYN010000163         4480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_19781_length_4480_cov_3.746441, whole genome shotgun sequence.
ACCESSION   DAOQYN010000163 DAOQYN010000000
VERSION     DAOQYN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19781_length_4480_cov_3.746441"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PKN80_06810"
     CDS             <1..461
                     /locus_tag="PKN80_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNQ35760.1"
                     /translation="IPQPDGSVHYFGAARMGAPMNAFVRISDQPIRLRSQIYRPDYVI
                     VADPTLCRSVDVLAGVKEKAVVVINAEKPLNLTPPSGGVKICYVPANEIARRHIGRPL
                     ANTALLGAFAAASGAVKLESLEEVIRRRFPEDLANKNIASLKEGWEVACR"
     gene            452..721
                     /locus_tag="PKN80_06815"
     CDS             452..721
                     /locus_tag="PKN80_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ35761.1"
                     /translation="MSININSRPGSARNNRTGSWRVDRRPKFLRQNCIACRLCLLICP
                     EGIITGKAKNSFETDYVYCKGCGLCAAVCPKKDIEMIPEDTETEG"
     gene            718..1872
                     /locus_tag="PKN80_06820"
     CDS             718..1872
                     /locus_tag="PKN80_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ35762.1"
                     /translation="MKDFIEGSDAVARAVALCRPGVISAYPITPQTHIVEFLASMVAD
                     GKLKSQFINVESEHSAASLVLGASAAGERVFSASSSQGLILMSEVLFNIAGMRMPVVL
                     ACVNRALSAPINIWNDQQDSMAVRDSGWIQLYAETGQECGDLIFQAYRLAESPEVRVP
                     VMVCLDGYLLSHGLEVVDVPEAAEVDKFLPPYRPAEKLDPAAPMTFGPLAGPDYYLEI
                     RHDLHAAMRGALKLIPSIAADFSRQFGRNSGGLIEGYRLEDAELAIVAAGSVCGTIKE
                     AIDALRQEGVKAGLLRLITYRPFPEEAVSAALAGVGRVAVLDKAISLGATGPIYADLA
                     TALVHGKNQARVNGYIAGLGGRDITIETIREIFTRLSGEEVSAGFVGLKG"
     gene            1899..2783
                     /locus_tag="PKN80_06825"
     CDS             1899..2783
                     /locus_tag="PKN80_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ35763.1"
                     /translation="MPEHLLSPGHTACAGCGQALAARLVIDTAGPNTIIVNNTGCLEV
                     FTTAYPESAWEVPFIHSLFENAGAVASGVESALRQTGRLDQIRVIAQAGDGGTADIGL
                     QALSGMWERGHDILSICYDNEAYMNTGIQRSGLTPTFTSTTTSPAGKASAGNPRPKKN
                     VMEIALAHGIPYVASASAGFPMDLQKKVKKALAIRGPKYLHIHVPCPLGWRHEPADTY
                     RVARLAVETGLFPMVEFEGGQQTGSYTLSAPKPVAEYLKLQGRFRHLFKPGAEAALQE
                     IQAIADRNIARYGLAKPA"
     gene            2834..3322
                     /locus_tag="PKN80_06830"
     CDS             2834..3322
                     /locus_tag="PKN80_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNQ35764.1"
                     /translation="MALIYSAEEIVRIAVEIENNGNRFYREAARLARSQPAVDLFKML
                     AEDEIKHRDYFESLGRQVPKSPLAEAYPGELELYLKALAEAAIFNQQKGFKAITAAAF
                     NEAQALELAVGAEKDSILYYHELLAFVPEDQKGHVRKILDEERRHLTSLRAFAQTLSG
                     QA"
     gene            complement(3331..4236)
                     /locus_tag="PKN80_06835"
     CDS             complement(3331..4236)
                     /locus_tag="PKN80_06835"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ35765.1"
                     /translation="MAQQKKKGRSPVIDAHNHPDWHGHNLEKFLANMDSFGIDQTWLL
                     SWECPADEYSNGSYCPPPMGPDGPIPFARCLSYAERAPDRFVLGYAPDPRRPEAIDRL
                     AAAIEIYGVRVYGELKLRMMYDNPDALRLFRFCGEKKVPVIVHLDYEIGENRNRYPRP
                     NWWYGGGIEAFERAIRKCPDTIFLGHGPGFWAHISKDGQHLVSYYPSGPVVREGEVTR
                     LMRRYPNLYADLSAGSGRKALNRDPGHAVEFLLEFQERILYARDCFDNGHQELLKSLK
                     LPAKTLAMILGGNARKLVPPGPALR"
BASE COUNT          783 a         1465 c         1450 g          782 t
ORIGIN      
        1 agatcccgca gcccgacgga tcggtgcatt atttcggcgc cgcccggatg ggggcgccca
       61 tgaacgcctt cgtccggatc tccgaccagc ccatccggct gcgttcgcag atttaccgac
      121 ccgattacgt gatcgtggcc gatccgaccc tctgccggag cgtcgacgtg ctggccggcg
      181 taaaggagaa ggccgtggtg gtaatcaacg ccgagaaacc tctgaacctg accccgccct
      241 ccggcggggt taaaatttgc tacgtgccgg ccaacgagat cgcgcggcgc cacatcggcc
      301 gcccgctggc caacaccgcc ctgcttgggg ccttcgccgc cgcttccggc gccgtgaagc
      361 tcgagagcct ggaggaggtc atccgccggc gttttccgga agacctggcc aacaagaaca
      421 tcgcttccct caaggaggga tgggaggtcg catgtcgata aacatcaact cccggcccgg
      481 cagcgcccgg aataaccgga ccggttcgtg gcgggtcgac cggcggccca agtttctccg
      541 ccagaactgc atcgcctgcc ggctctgcct gctgatctgc ccggaaggca tcattaccgg
      601 caaggccaag aatagtttcg agaccgatta cgtctactgc aaggggtgcg gcctctgtgc
      661 ggcggtctgt cccaagaagg acattgagat gattcccgaa gacacggaga cagaaggatg
      721 aaagatttca tcgaaggttc cgatgcggta gcccgcgccg tcgccctttg ccggcccggg
      781 gtgatctccg cctacccgat cacgccccag acccacatcg tggagttcct ggcttcgatg
      841 gtggccgacg gcaaactgaa gagccagttc atcaatgtcg agagcgagca ttcggccgcc
      901 tcgctggtcc tgggggcttc ggccgccggc gagcgggtct tctcggccag ctcctcgcag
      961 gggttgatcc tgatgagcga ggtcctcttc aacatcgccg gcatgaggat gccggtggtg
     1021 ctggcctgcg tcaaccgggc cctttcggcc ccgatcaaca tctggaacga ccagcaggat
     1081 tcgatggccg tccgtgacag cggctggatc cagctttacg ccgagaccgg ccaggagtgc
     1141 ggcgacctga tcttccaggc ctaccggctg gccgagtcgc ccgaggtccg ggtgccggtc
     1201 atggtctgtc tcgacggcta cctgctcagc cacgggctgg aggtggtgga cgttccggaa
     1261 gcggccgagg tggacaagtt tctgccgcct taccggccgg ccgaaaaact ggacccggcc
     1321 gcgccgatga ccttcggccc gctggccggc cccgattatt acctggagat acgtcacgac
     1381 ctccacgcgg ccatgcgcgg cgccctgaag ctcataccct ccatcgcggc cgatttttcc
     1441 aggcagttcg gacggaactc gggcggcctg atcgagggat accggctcga ggatgctgag
     1501 ctggccatcg tggccgccgg gtcggtctgc ggcaccatca aggaggcgat cgatgccctg
     1561 cgccaggaag gtgtcaaggc cggcctgctg cgcctcatca cctaccggcc cttcccggag
     1621 gaggcggtga gcgccgccct ggccggggtc ggccgggtgg cggtgctgga caaggccatt
     1681 tcgctgggcg ccaccggacc catctatgcc gacctggcca cggccctggt ccacggcaag
     1741 aaccaggccc gggtcaacgg ctacatcgcc ggcctgggcg gccgggacat caccatcgag
     1801 accatccgtg agatattcac ccgcctgtcc ggcgaggagg tcagcgccgg tttcgtggga
     1861 ctcaaaggct gaacgcggaa aaactgagga gaagagagat gcctgaacac ctgctttccc
     1921 ccggccatac cgcctgtgcc ggctgcggcc aggccctggc ggcccggttg gtgatcgaca
     1981 ccgccggtcc gaacaccatc atcgtgaaca acaccggttg cctggaggtc ttcacgaccg
     2041 cctatccgga atcggcctgg gaggtgccct ttatccactc cctcttcgag aatgccggcg
     2101 cggtggcctc gggcgtggag tcggccctgc gccagaccgg ccggctcgac cagatccggg
     2161 tgatcgccca ggccggcgac ggcggcaccg ccgacatcgg tctccaggcg ctttccggca
     2221 tgtgggaacg gggtcacgac atcctgtcga tctgctacga caacgaagcc tacatgaaca
     2281 ccgggatcca gcgcagcgga ttgacgccga ccttcacctc gaccaccacc agcccggccg
     2341 gcaaggcctc cgccggcaac ccgcggccca agaaaaacgt gatggagatc gccctggccc
     2401 acgggattcc ctacgtggcc agcgcctcgg ccggcttccc gatggacctg cagaagaagg
     2461 tcaagaaggc gctggccatc cgcggcccga agtacctcca catccacgtg ccctgtccgc
     2521 tcggctggcg ccacgagccg gccgacacct accgggtcgc ccggctggcc gttgagacgg
     2581 ggctctttcc aatggtcgaa ttcgagggcg ggcagcaaac cggcagctac accctatcgg
     2641 ctcccaagcc ggtcgccgag tacctgaagc tgcagggacg tttccgccat ctcttcaagc
     2701 ccggcgccga agccgcgctc caggagatcc aggcgatcgc cgaccgcaac atcgctcgct
     2761 acggcctggc caagccggcc tgatcgatcg gaaaaaccag ggctcgggaa gggtcaactt
     2821 cgggaggtat gggatggcgt tgatatattc cgcggaagag atcgtccgga tcgcggtgga
     2881 gatcgagaac aacggcaacc gtttttaccg ggaagccgcc cggttggctc gttcgcagcc
     2941 ggccgtcgac ctctttaaaa tgctggcgga ggatgagatc aagcaccggg attacttcga
     3001 atcgctcggc cggcaggttc cgaagtcgcc cctggccgag gcctacccgg gcgagctgga
     3061 actctacctg aaggcgctgg ccgaagcggc catctttaac cagcagaaag gtttcaaggc
     3121 catcaccgcg gccgctttca acgaggccca ggccctggaa ctggcggttg gggccgaaaa
     3181 ggactcaatc ctttattacc acgaattgct ggccttcgtt cccgaggatc agaaggggca
     3241 tgtccggaag atcctggacg aagaacggcg ccatctcacc agcctccgcg ctttcgccca
     3301 gacactctcc gggcaggcat aaggggaggt ttaccgtaag gccgggcccg gcggcaccag
     3361 tttccgggcg tttccgccca ggatcatggc cagggtcttc gccggcagtt tcagcgattt
     3421 cagcagttcc tggtggccgt tgtcgaagca gtcccgggcg tacagtatgc gctcctggaa
     3481 ctccagcagg aattcgaccg cgtggcccgg gtcccggttg agggccttgc ggccggagcc
     3541 ggccgagaga tcggcgtaaa gattcggata ccgccgcatc aaccgcgtca cctcaccctc
     3601 cctgaccacc ggtcccgacg ggtagtaaga gaccaggtgc tggccgtctt tcgagatgtg
     3661 ggcccagaag ccggggccgt gaccgagaaa gatggtatcc gggcatttcc ggatggcccg
     3721 ctcgaaggct tcgatgccgc ctccgtacca ccagttgggc ctcggatagc ggttccggtt
     3781 ttcaccgatt tcataatcca ggtggacgat caccggtacc ttcttctctc cgcagaagcg
     3841 gaagaggcgg agggcgtcgg ggttgtcgta catcatccgc agtttcagct ccccgtaaac
     3901 cctcaccccg tatatttcaa tcgcggccgc cagccggtcg atcgcctcgg gccgcctcgg
     3961 atccggcgcg tagcccagga cgaaccggtc gggcgcccgt tccgcgtagg aaaggcagcg
     4021 ggcgaagggt atcgggccgt ccgggcccat cggcggcggg cagtaactac cgttgctgta
     4081 ttcgtcggcc gggcactccc agctgagcag ccaggtctgg tcgatgccga aagagtccat
     4141 gttggccagg aacttttcca ggttgtggcc atgccagtcc gggtgattgt gggcatcgat
     4201 gaccgggctc cgcccctttt ttttctgctg cgccatcgtt gggctccttt cgtggtttgg
     4261 atgggggtgg ccggtttccg gggcccgggc tggactccgg gtgttgcgtt tctcaggcct
     4321 tgaagacggt ccggccgccg cagacggtgc ggacgagccg tcccttgagg cggcggccga
     4381 ggaagggcgt gttgcggctg cggctcagga tcagctccgg ggtcaggacc cagcttgatt
     4441 ccgggtcgta gatggccagg tcggcccggt aaccgggctg
//
LOCUS       DAOQYN010000164         4466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_19878_length_4466_cov_3.951938, whole genome shotgun sequence.
ACCESSION   DAOQYN010000164 DAOQYN010000000
VERSION     DAOQYN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19878_length_4466_cov_3.951938"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2709)
                     /locus_tag="PKN80_06840"
     CDS             complement(<1..2709)
                     /locus_tag="PKN80_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35766.1"
                     /translation="MHFKRERSGRRFRSLLASLLGAAAVAGWLAGPAAADPLFVNKLK
                     AEPAVPVELVFEAEEFPGLPVYPDFHRGAGQGFYVKEHTHASGRAMVVCDSESERATI
                     VKTFDRRLPAGDYLLGLCIVLTRWTRDVVTQYETPSGNIIRVELGLADGEGFRPVAIM
                     RLQGLFGSGYCFLPRVTSVRTQYGENPRTGMDTARTTAVFEDPVLKVPEEWNAIRITA
                     EKVISGGIGDVPEFALPYIILDRVKITNRPGYEWNFNARGRYGLLLPSELKEAALKAQ
                     SAPPVPGAPAGQGAPAPAAAPAPAGNLVCDGSFEAGGRPWWSPAMDGTDDFTFDPQDI
                     IAGASPHGDRFARLALVQAQLYADDDARPKAYRAAIRSAEFTLPAGGGVVSFFARASV
                     TPATLRLAWMDYTGAGKARQRRVLGRTEFTLDREWKQYSYRIESAAPAPVSLILAGES
                     AANGALVDIDAVQVLAPGVDSRDYREGEGVRLLIANPAPYGTFHDNGPVRFQAVLVNG
                     TSAGQTRRLRYRVTDLAGGLYGEGTVEVKGGKGITRRDFVLADFKGYGNFVLVAGPEA
                     GPGPDRQLIPFTRVPEPARVEKDSRNPVYLGTLGRNIRGRSGRMMRHYGYEFTTTLND
                     VILNAGSNTLGYQRHAVYRNEVEQTRSTGLQWIPWIFPAQPAPYDITVLSSGPAATTR
                     PWMSPEFGAWYTQWILERYQGLYGDMFIVTDELTNNTVPGSALPYVHSAYRAIKQKDP
                     RLQVMNSIEFPPSEAFARELGGVKGVWTDALGGSRYGTDKWWYAREARFVREKQLPFY
                     VTAVGWYFNYADLEYLTPDGRPLDPAKLFHTMNAQAWDLAAITAIYRPMRYSVYTGKY
                     GSMATDPFNQFALYDGRPTAMAAQWIVLEQFMREAAPGELLRLKQAS"
     gene            complement(3615..>4466)
                     /locus_tag="PKN80_06845"
     CDS             complement(3615..>4466)
                     /locus_tag="PKN80_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35767.1"
                     /translation="RRPVTLAVGGGRETYREVAGFPLAGASSPEAVEAYPGWPSADWF
                     DYGAIEAQCEAVRRQGRVAVFMGDRLNRVAQLKPAMYLRGVEQILLDLSLEPELARAV
                     LGRIRKFYLEYARRIFEAARGKLDILLMGDDFGCQQGPLLSPKMWLDFLGEGFAAYIG
                     LAHAYGLRVMHHTCGSVRPIVPLMLERGLDVLQSLQPEAAGMDHRALKAEFGGRLAFH
                     GGISIQQTLPFGRPDQVRREVRDRVEALGPGGGYILGTSHNIQADAPVENVLALLEAY
                     REYAPYR"
BASE COUNT          806 a         1500 c         1418 g          742 t
ORIGIN      
        1 gctggcctgt ttcagccgca gcagctcgcc cggggccgcc tcccgcatga actgttccag
       61 gacgatccac tgggcggcca tggccgtcgg ccggccgtcg taaagggcga actggttgaa
      121 ggggtcggtc gccatgctgc cgtacttgcc ggtatagacc gagtagcgca tcggacggta
      181 gatcgcggtg atggcggcca ggtcccaggc ctgggcgttc atggtatgga agagcttcgc
      241 cgggtccagc ggccggccgt cgggagtcag gtactccagg tccgcgtagt tgaagtacca
      301 gcccaccgcg gtcacgtaaa acggcagctg cttttcgcgc acaaagcggg cctcccgggc
      361 gtaccaccac ttgtcggtcc cgtaccggct gccgcccagc gcgtcggtcc agaccccctt
      421 gaccccgccc agttcccggg caaaggcctc ggagggcgga aactcgatcg agttcatgac
      481 ctgcaggcgg gggtccttct gcttgatcgc ccggtaagcc gaatgcacgt aaggcagggc
      541 gctgccgggg accgtgttgt tggtcaactc gtcggtcacg atgaacatgt ccccgtacag
      601 cccctggtac cgctccagta tccactgcgt gtaccaggcg ccgaactccg gcgacatcca
      661 gggccgggtc gtggccgccg gcccggaact caggacggta atatcgtagg gcgccggctg
      721 ggccgggaag atccagggta tccactggag gccggtggac cgggtctgct ccacctcgtt
      781 ccggtaaacg gcatgccgct ggtagccgag cgtgttggag ccggcattca ggatgacgtc
      841 gttgagcgtg gtggtgaact cgtagccgta gtggcgcatc atccgccccg agcgcccccg
      901 gatgttccgg ccgagggtgc ccaggtagac cgggttccgc gagtcctttt cgacccgggc
      961 cggctccggc acccgcgtaa agggaatcag ctggcggtcc gggccggggc cggcctcggg
     1021 accggccacc aggacgaaat tgccgtatcc cttgaaatcg gccaggacga aatcgcgccg
     1081 ggtgattcct ttgccgccct tgacctccac cgtcccctcc ccgtagaggc cgccggccag
     1141 gtcggtgacc cggtagcgga ggcggcgcgt ctggccggcc gaagtgccgt tcaccaggac
     1201 cgcctggaag cgcaccggcc cgttgtcgtg gaaggtgccg tagggcgccg ggttggcgat
     1261 gagcagccgg accccctccc cctcccggta atcccgggaa tcaactcccg gcgccagaac
     1321 ctggaccgcg tctatgtcca ccagcgcgcc gttggcggcc gactcgccgg ccaggatcag
     1381 gctgaccggg gccggggcgg ccgattcgat ccggtaggag tactgcttcc attcccggtc
     1441 aagcgtgaat tccgtccggc ccaggacgcg gcgctggcgc gccttgcccg ccccggtata
     1501 atccatccag gccaggcgga gcgtcgccgg cgtaacggaa gcccgggcga agaaactgac
     1561 gacgcccccg ccggccggca gggtgaactc cgcgctgcgg atcgcggccc ggtaggcctt
     1621 cggacgcgcg tcgtcgtccg cgtacagctg cgcctggacc agggccagcc gggcaaaacg
     1681 gtcgccgtgc ggagaggcgc cggcgatgat atcctgggga tcgaaggtga aatcgtccgt
     1741 cccgtccatg gccgggctcc accaggggcg gccgcccgcc tcgaaactgc cgtcgcaaac
     1801 caggttaccg gccggagccg gggccgcggc cggagccggg gcgccctggc cggccggggc
     1861 gccgggcacc ggcggcgccg actgcgcctt gagcgccgcc tccttgagct cggagggcag
     1921 cagcagcccg taacggccgc gcgcgttgaa gttccactcg taaccgggcc ggttcgtgat
     1981 cttgacgcgg tcaagaatga tatagggcag ggcgaactcc ggcacatccc cgataccgcc
     2041 ggagatcacc ttctcggcgg tgatgcggat ggcgttccac tcctcgggca ccttgagcac
     2101 cgggtcctcg aagacggccg tggtgcgggc ggtatccatc cccgtccgcg gattctcccc
     2161 gtactgcgtc cgcaccgagg tcacccgcgg caggaagcag taccccgaac cgaagaggcc
     2221 ctgcaggcgc atgatcgcca ccggcctgaa cccctcgccg tcggccaggc cgagttcgac
     2281 gcggatgatg tttcccgacg gcgtctcgta ctgggtgacc acgtcccggg tccagcgggt
     2341 cagtacgatg cagaggccca gcaggtaatc cccggccggc agtcgccggt cgaaagtctt
     2401 aactatggtt gcccgttcgc tctccgaatc gcagacgacc atcgcccggc cggaggcgtg
     2461 cgtgtgctcc ttgacgtaga agccctggcc ggccccgcgg tggaaatccg ggtaaaccgg
     2521 caggccgggg aactcctcgg cctcgaaaac gagctccacc ggaacggccg gctccgcctt
     2581 gagcttgttg acgaagagcg gatcggccgc ggccggaccc gccagccagc cggccaccgc
     2641 agccgcgcca agcaaagatg ccaggagact ccgaaaccgg cggccgcttc tctcgcgctt
     2701 aaaatgcatt ctgctcctcc tcgttttgtt ttgtcaggct ggaaatcgga atttaatgcc
     2761 tggaatcatg cccggaatgc gtcaattgca tacccggtaa taatatacca ccttttcgtg
     2821 tgccgttcac acgcccccgt ccccacggat agttggtggg gacccaaatc cataaaatgt
     2881 ccgtcattcc ctcgtgtggt aagagggaat ccagcattta atgcctttca ggttttccag
     2941 gtagcctgga tacccgctaa ccacctgcgg gtatgacaaa cggaaaaagc cgtcattccc
     3001 gtatgtggta aacgggaatc caagtccaat gtattcaatt aaccctcagc agtaagcggg
     3061 cctgcctacg ctaaagctac ggcaggtaaa ccatgtccgc cgaagccctc ttgcactata
     3121 aaaccgctcc ggaaggtgaa ctcgtgaagt aggcggcctg cctacactaa agtttatccg
     3181 ccgtagccct cctgcacttt aaaacagttc cggcgggtaa acacgtaacg caggcgggct
     3241 tgcccgccga agctcgcttt gcgagcgtag gcggaaatcc aatccttaaa cgtttgtcat
     3301 tccctcgtgt agcaagaggg aatccagcat ttaatgcctt tcgggttttc caggtagcct
     3361 ggatacccgc taaccacctg cgggtatgac aaacggaaaa agccgtcatt cccgtatgtg
     3421 gtaaacggga atccaagttc aatttattta ataatcccca agttggcgga acgccaacct
     3481 ttaaagcttt tcgggttttt caagtagcct ggatccccga ctaaagcatt cggggatgac
     3541 gtgcaaaaac aggcgggtga tcaaaccgtg aatacgcggc ttacttgccg gcgcccggac
     3601 agtaattcaa ataatcaccg gtacggcgca tactcccggt aggcctccag cagggccagg
     3661 acattctcca ccggcgcgtc agcctggata ttgtgtgagg tacccaggat gtagccgccg
     3721 cccggcccga gcgcctccac ccggtcgcgg acctcccggc gcacctggtc cggcctgccg
     3781 aagggcagcg tctgctggat ggagatgccg ccgtgaaagg cgaggcggcc gccaaactcc
     3841 gccttgaggg cccggtggtc catgccggcc gcctccggct ggagcgactg gagcacgtcc
     3901 aggccgcgct ccagcatcag cggcacgatc ggccggaccg aaccgcaggt atggtgcatc
     3961 acccgcaggc cgtaagcgtg ggccaggccg atgtaggcgg caaaaccctc gcccaggaag
     4021 tccagccaca tcttcggcga gagcaagggc ccctgctgac agccgaaatc gtcgcccatc
     4081 agcaggatat ccagcttccc ccgggcggcc tcgaagatgc gccgggcgta ctccaggtaa
     4141 aacttccgga tgcggcccag gaccgcccgc gccagctccg gctccaggct gagatcgagc
     4201 aggatctgct ccaccccgcg caggtacatg gccggcttca gctgcgccac ccggttgagc
     4261 cggtcgccca tgaagaccgc caccctgccc tgccgccgca ctgcctcgca ctgggcctcg
     4321 atggcgccgt aatcgaacca gtcggccgag ggccagccgg gataggcctc gaccgcctcc
     4381 gggctggagg caccggccag cggaaagccg gccacctccc ggtaggtctc ccggccgccg
     4441 ccgaccgcca gggtaaccgg ccgccg
//
LOCUS       DAOQYN010000165         4434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_20089_length_4434_cov_4.477963, whole genome shotgun sequence.
ACCESSION   DAOQYN010000165 DAOQYN010000000
VERSION     DAOQYN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20089_length_4434_cov_4.477963"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..1612
                     /locus_tag="PKN80_06850"
     CDS             284..1612
                     /locus_tag="PKN80_06850"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNQ35768.1"
                     /translation="MKEVEKVLKRAVDEKVKFVNLWFVDILGQLKSVTIRDRELEKAL
                     SEGIGFDGSSVEGFARIYESDLLLKPEAETFRILNWCSTPQERVAAFFCDILTPEGEG
                     YAGDGRLALKRVMEQARRQGFNFCVGPELEYFYFKDNGSVPGLTDQGGYFDFAPIDLG
                     SDLRQETVSLAEDMGIEIDTGHHEVAPSQHELDLRYGNALELADDIITVKMLTKEVAR
                     RHNLYASFMPKPVFGENGSGMHVHMSLFRGRENAFFDDRDPDKLSAVARGFAAGVLAH
                     SREVTAICNQWVNSYKRLVPGYEAPVYIAWARSNRSSLVRIPAFKHGKPTAARIEFRS
                     PDPACNPYLAFAVVLAAGLAGIEKGLQLPAPVEADIFSMDEKTRKRHHINHLPGSLIE
                     AILETGRSRLVRAALGDHIFEKFIENKQIEWDRYRTQVTDYELKAYLPIL"
     gene            1646..2128
                     /locus_tag="PKN80_06855"
     CDS             1646..2128
                     /locus_tag="PKN80_06855"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNQ35769.1"
                     /translation="MHYLIDGYNLIRQVPELQAAEAESLEAGREALLSRLDIFRRRHP
                     ATRITVVFDGPAGVFPPAGGPAGVRVLYSRGRKADDWIKELIDASDDPGELVVVSADR
                     EVVDYARLARCRIQAPLPFSRRFFEVPKAADRKPLIEPKGLHRIEEELRRRWIRKDGR
                     "
     gene            complement(2646..2846)
                     /locus_tag="PKN80_06860"
                     /pseudo
     CDS             complement(2646..2846)
                     /locus_tag="PKN80_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200278.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
     gene            complement(2876..3565)
                     /locus_tag="PKN80_06865"
     CDS             complement(2876..3565)
                     /locus_tag="PKN80_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HNQ35770.1"
                     /translation="MLLEITDLKKEYPGPGGAGRQTVLAGVDLGLERGEVVGVLGESG
                     SGKSTLARMLLRLEAPTAGRLRYDGQDLLALKPAQLLAWRKKVQIVLQNPFRSLPPRK
                     RVGDILREVVRFHRVRPREEEDAYIGELLEQVGLPTGAAGRFPFQFSGGERQRVAIAR
                     SLAVEPEFLLLDEPTSNLDLSIQAQVINLLADLRRRRNLTYLLITHDRPLAAYFCDRR
                     LGLEEGRLSPM"
     gene            complement(4033..4233)
                     /locus_tag="PKN80_06870"
                     /pseudo
     CDS             complement(4033..4233)
                     /locus_tag="PKN80_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
BASE COUNT          728 a         1420 c         1478 g          808 t
ORIGIN      
        1 cgagtccgag gtctgtccga agtgcgccca ggccctggcc cggttgggcc gggacggcca
       61 ggccagcccg gccgcctgag gtccttgttt caggagaagg catgaaagag atcaacttga
      121 agttggacgc ctggttcggg tcgtttcggc gccgggactg cgcctcttta ccggccgcgc
      181 acccggcccc ggaggatagc cccagagata gccaggcctg ggcccggccg ggccgcggcg
      241 gccaagccag acaattttcc tgaaaccggt ttctggagga acgatgaaag aagtggaaaa
      301 ggttttaaag cgggcggtcg atgagaaggt caagttcgtc aacctctggt tcgtggatat
      361 cctgggccag ctcaagtcgg tgaccatccg ggaccgggag ctcgagaagg ccctcagcga
      421 gggcatcggc ttcgacggct cctcggtcga gggcttcgcc cggatttacg aaagcgacct
      481 gctgctcaag ccggaagcgg agaccttccg gattttgaac tggtgttcga cgccccagga
      541 gcgggtggcc gccttcttct gcgacatcct gacgcccgag ggcgagggct acgccggcga
      601 cggccggctg gccctgaagc gggtgatgga gcaggcccgc cgccagggct tcaacttctg
      661 cgtcggcccg gagctcgaat acttctactt caaggacaac ggctcggttc ccggcctgac
      721 cgaccagggc ggctactttg atttcgcgcc catcgacctg ggttccgacc tgcgccagga
      781 gaccgtcagc ctggccgagg acatgggcat cgaaattgat accggccacc acgaagtcgc
      841 ccccagccag cacgaactgg acctgcgcta cgggaatgcg ctggaactgg ccgacgacat
      901 catcaccgtc aagatgctca ccaaggaggt ggcgcgccgt cacaacctgt acgcctcctt
      961 tatgcccaag ccggtcttcg gcgagaacgg cagcggcatg cacgtgcaca tgtcgctctt
     1021 ccggggccgg gagaacgcct tcttcgacga ccgcgacccc gacaagctct ccgcggtggc
     1081 ccgcggcttc gcggccgggg tgctggccca cagtcgggaa gtcaccgcca tctgcaacca
     1141 gtgggtcaac tcctacaagc ggctggtgcc cgggtacgag gctccggtct atatcgcctg
     1201 ggcgcgcagc aaccgcagtt ccctggtgcg catcccggcc ttcaagcacg gcaagccgac
     1261 cgcggcccgg atcgagttcc gcagcccgga cccggcctgc aacccctacc tggcctttgc
     1321 cgtcgtcctg gcggccggcc tggccgggat tgagaaaggg ctccagctgc cggcgccggt
     1381 cgaggccgac atcttcagca tggacgagaa gacccgcaag cgccaccaca tcaaccacct
     1441 gcccggcagc ctgatcgagg cgatcctgga gaccggccgg agccggctgg tccgcgcggc
     1501 gctcggcgac cacatcttcg aaaagttcat cgagaacaag cagatcgagt gggatcgcta
     1561 ccgcacccag gtgaccgact acgaactcaa ggcctacctg cccattcttt aagggtgaga
     1621 gtgcggcctt gattccggcg gccccgtgca ctacctcatt gacggctaca acctgatccg
     1681 gcaggtgccg gaactgcagg ccgccgaggc cgagagcctg gaggccggcc gggaggcgct
     1741 cctgtctcgc ctggacatct tccggcgccg ccacccggcg acgcggatca ccgtcgtctt
     1801 cgacggcccg gccggggtgt ttccgccggc cggcggcccg gccggggtcc gggtgcttta
     1861 ctcgcgcggc cgcaaggccg acgactggat taaggagttg atcgacgcca gcgacgatcc
     1921 gggcgagctg gtggtggtga gcgccgaccg ggaggtggtt gattacgccc ggctggctcg
     1981 ctgccgcatc caggcgccgc tgcctttcag ccggcgtttc ttcgaggtgc cgaaggccgc
     2041 cgaccgcaag ccgctcatcg agccgaaggg gctgcaccgg atcgaggagg aacttcgtcg
     2101 gcgctggatc aggaaggacg gacgttaagg cgtggccgac cggggccggc gggttctccg
     2161 ccggcccctt ttttttgtgc tcccgttttc atccggggcg aagggagggt ccgttccggc
     2221 cctggcggcc gcggggaatt cccggccggg aatcaaggct gctggccggg gttgcgcagg
     2281 gtgccggtgt accggtcggc gatctgaccg agcggtatcc agttgacatg gctgcccacg
     2341 aagagcatgt tgaccccttc ggcgccatgg ttggcccgca cgttcaaggc cgggttccag
     2401 tcggtgttct tgtgcaggtt aaggatggtc agggagacgt cggccatcag cggcgagtca
     2461 ttcttcgact gttcggagag gcgcccctgg taggcgtagg agaggttgac cggatctccg
     2521 tcgtccagct ggtcccaggt ttcggccgcg gtgtcccact tggcggccgg gcagaccagt
     2581 agcttggtgg ccttggcgta cggttccagc aggaccagta tggacttgac ctgggtgctg
     2641 ccggcgatgt gcgggaagcg ttctcggtaa tcctgggcgt acatgtgcat ggccagtccc
     2701 agctgcttga ggttgttcag gcagctggtc cggcgggcct gttcgcgggc ccgggccagg
     2761 gccggcagca gcatcccggc caggatggcg atgatggcga tgaccaccag cagctcaatc
     2821 agcgtaaacc ccttcctctt cgacatttcc gcctcctttt ttgacctcgg gcctttcaca
     2881 tcggggaaag ccgtccctct tccaggccca gccgccggtc gcagaagtag gcggccaggg
     2941 gccggtcgtg ggtgatcagc aggtaagtca ggttgcgccg gcggcgcagg tcggccagca
     3001 ggttgattac ctgggcctgg atcgagaggt ccaggttgct ggtcggctcg tccagcagca
     3061 ggaactccgg ttcgaccgcc aggctccggg cgatggccac ccgctgccgt tcgccgccgg
     3121 agaactggaa ggggaaacgg ccggccgcgc cggttggcag gccgacctgt tccaggagtt
     3181 cgccgatgta ggcatcctcc tcttcccgcg gccgcacccg gtgaaaacgg accacctccc
     3241 gcaggatgtc gccgacccgc ttgcgcggcg gcagggagcg gaatgggttc tggagcacga
     3301 tctggacctt tttccgccag gccagcagct gggccggctt gagcgccagc aggtcctggc
     3361 cgtcgtagcg gaggcggcca gccgttggcg cttcaaggcg cagcagcatc cgggccaggg
     3421 tgcttttgcc cgagccgctt tcgcccagta cgccgaccac ctcgccccgc tccagcccga
     3481 ggtcgacccc ggccagcacc gtctggcggc cggcgccgcc cggtccggga tactccttct
     3541 tcaggtccgt gatttccagc agcattttct gattatagca cggccgccgg ccggtgccga
     3601 aaccgccgga ggccagaaag gcgggggttg aacatttcaa cccccgccag atggacttgc
     3661 cctggccgtc ccttacgggt tagtcagagt ggcgccgccg atgttgtttg acaactggcc
     3721 ggacggcgac caggatacgt gcccgtcaat gtacaggatg ttgatcccgt cggtgctgtg
     3781 gatgttggca ctggtcagcg cagtggccga ggtgatggtc ccggagttgt ccgtcttgtc
     3841 gcacatcaac ggggaatccg gcgccgtctg ctcgttggcg ttgagctgat aggcgtagga
     3901 gatctgcgtc tccgtcagcg tcgcaaccgt ggcaccgaac gcaccggcct ggtcactcgg
     3961 gcaccagaag agcttcggcg ccgaggcgta gttcgccagt agctgcatgt cgttgaccac
     4021 gttgtttatg ggcgccttcg ggaagatctc ctcgtactcc tgcgagtaca tatgcatggc
     4081 caggccaagc tgtttcaggt tgctgagaca gttggagcgc cgagcctgct ctctcgccct
     4141 ggcaagtgcc ggcagcagca tcgccgccag gatagcaatg atggcaatta ccaccaagag
     4201 ttcgatcagg gtgaacccct ttctcgtctt cactttactt cacccccttt cggtcctttt
     4261 gttcgcgcgg acgcttccgc gccggcgcgc tagtcctttt cccccaacga tacaatagag
     4321 ttattgtatc acctgtatta taccgaaggg gggggagttt gtcaagcgcg gcggttttgg
     4381 gttcgttccc actctgcgtc cggggcaaaa gctacgcgac tcgggaacga gagc
//
LOCUS       DAOQYN010000166         4428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_20122_length_4428_cov_4.193689, whole genome shotgun sequence.
ACCESSION   DAOQYN010000166 DAOQYN010000000
VERSION     DAOQYN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20122_length_4428_cov_4.193689"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1682
                     /locus_tag="PKN80_06875"
     CDS             <1..1682
                     /locus_tag="PKN80_06875"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNQ35771.1"
                     /translation="LYRALVKTGLAYSTGAYAYTPRDPGLFIITAVCQPDNLDAVQAA
                     IRREAAGLVRLSEAEIKRARTRLETRSIFGLESLEGRSQDLASSLHLTGSPLFLDAYL
                     AGLARIDRRRLARAAADYLKEENSTRSILVPEEKTGIDTPPADPSRPGTVRVFRLENG
                     TVLLAKRNNNLPLVSISLLMKGGRLFETEKSGGSFDLLAEMLKAGTRKLDARAVADRV
                     ADWGGSLNPYAGNNSFGLTLEAPSRYWRDVLSLMSELLIRPGFPDQELDRLREESIGA
                     VTARDENDPARALNALKKSAFGEHPYGLPGGGSITSLKAIDRTGLLELYRNFVRSDNL
                     VITVLGDIDPDEIAREAGRRFRSLPSGSRLAPPPLPEAAPGGRLELETRKRESVVMIG
                     FPGLAVDSPERGCLELLAEILNGQEGLLFQRLREREPLVYSSSFSYFLGLQPGLLFFQ
                     AQCRPERVGRVTEILNGIIEELRTRDFPGDQLAAARRRVVGTRLIGRQTLSSQALDYG
                     LNQLYGLDWDYSARLESGLPGIGTGELAGFCRRFLDPNRAIVVTTVPSKTE"
     gene            1679..2050
                     /locus_tag="PKN80_06880"
     CDS             1679..2050
                     /locus_tag="PKN80_06880"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNQ35772.1"
                     /translation="MKKRPKLDPGVILALTAQVPPGRLTTYGTLARIAGRPGGARAVG
                     QVLKRNPDPERVPCYRVVRSDGRLGGYHGTSAAGLKRKAARLQADGIELIPGPEPRVR
                     NLERLLFKAFRTPGRGQIKRL"
     gene            2095..3576
                     /locus_tag="PKN80_06885"
     CDS             2095..3576
                     /locus_tag="PKN80_06885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HNQ35773.1"
                     /translation="MRYLACDLGAESGRIVAGSLGKGRLNLELVHRFSNQPVWLPGGL
                     RWDTLGIFREIVSGLVRYRQAHRTELAGISCDSWGVDFGLLDKRSQLVGNPNHYRSRR
                     FEGAMEKVLAILPAGEIYRRTGIQFLPFNTIYQLYTLASECDPALEISRKLLMTADLV
                     NFFLSGEAAQEFTLATTSQLYDPNRRGWAAGLFRKLDLPLKLMPAVTPPATVIGKLRW
                     RIGSETGLAGTPVIAGASHDTAAAVAACPARPGERFAYLSSGTWSLLGVELERPLINE
                     TVRAEGFTNEGGLGGTYRFLHNITGLWILQECRRAWQHEGRLYEYPELTALAAGARPF
                     QALINPDHPDFLAPEKMPEAVAGFCRKTGQRPPPDRGGMVRVILESLALRYGQFVRTL
                     NRLGALAGPLEVLYVVGGGSQNELLNQFTANATGIPVVTGPVEATAAGNILAQALAAG
                     EIASIWEGRATVRAAHDLKTYRPDGRERWTEMLNQMEKVCSMA"
     gene            3561..>4428
                     /locus_tag="PKN80_06890"
     CDS             3561..>4428
                     /locus_tag="PKN80_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HNQ35774.1"
                     /translation="MFHGLTTRTFPGGVHPPESKYTSTSASLEAPRPEKAVLMLSQHT
                     GAPARSLVAAGDRVATGQKVGEKTGFISASVHSSLTGVVKAVEMRTGPAGNRTEAVVV
                     EASGPDEFRFLEPLDWTTATPEALRERVGEAGIVGLGGAAFPTEVKLAPPRPVDTLLV
                     NGCECEPYLTADHRLMLEKPVPVLTGALFSARILKVDSVVVGVEDNKPDALEQLSRAA
                     RELAGNGIKIRVEAVRSKYPQGAEKNLIQVLLKRLVPPGKLPMEVGAVVSNVGTALAV
                     CEAVMLGKPLYER"
BASE COUNT          801 a         1462 c         1423 g          742 t
ORIGIN      
        1 gcctttaccg cgccctggta aagaccggcc tggcctattc gaccggcgcc tatgcctaca
       61 cgccgaggga ccctggtctc ttcatcatca ccgccgtctg tcagccggat aacctggacg
      121 ccgtccaggc cgccatccgg cgcgaagcgg ccggcctggt ccgcctctcg gaagcggaaa
      181 taaaacgcgc ccggacccgc ctggaaaccc gttccatctt tggactggaa agcctggagg
      241 ggcgttctca ggacctggcc tccagcctcc acctgaccgg cagcccattg ttcctggacg
      301 cctacctggc cgggctggcc cggatcgacc ggcgacggct ggcccgggcg gcggctgatt
      361 acctgaaaga ggaaaacagt acccgctcca tcctggtccc cgaggaaaaa acgggtatcg
      421 acacaccgcc ggccgacccg tcacgccccg ggacggtccg cgtcttccgc cttgaaaacg
      481 ggaccgttct gctggcaaaa cgaaacaata acctgccgct ggtttcgata tccctgttga
      541 tgaaaggcgg ccgcctcttt gaaaccgaaa aaagcggggg cagcttcgac ctgctggctg
      601 aaatgctgaa agccggcacc cggaaactgg atgcccgggc cgtcgccgac cgggtggccg
      661 actggggcgg ttctctgaac ccttacgccg ggaacaacag tttcggcctg accctggaag
      721 caccctcccg ttactggcgc gacgtattgt ccctgatgtc tgagctgctc atccggcccg
      781 gctttcctga tcaggaactc gaccggctgc gggaagaatc catcggcgcc gtcaccgccc
      841 gggatgaaaa cgacccggcc cgggccctga atgccttgaa aaaatccgcc tttggagagc
      901 atccctacgg tctgcccggc ggcgggagca tcaccagcct gaaggccatc gatcgaaccg
      961 gcctcttgga actataccgg aacttcgtcc ggtccgacaa cctggtgatc accgtgctgg
     1021 gcgacatcga tccggacgaa atcgcccggg aagccgggcg gcgcttccgg tctctgccgt
     1081 ccggaagccg gctggcgccg cccccgttgc cggaggcggc tccgggtggc cggctggagc
     1141 tggagacccg gaaacgggaa tcggtggtca tgatcggctt ccccgggctg gccgtcgaca
     1201 gccccgaacg cggctgcctg gaactgctgg ccgaaattct caacggccag gaaggcctgc
     1261 tcttccagcg gctccgggaa agggaaccgc tggtgtacag cagcagtttc agctactttc
     1321 tcggcctgca gcccgggctt ctattcttcc aggcccagtg ccgacccgag cgggtagggc
     1381 gggtaaccga aatcctgaac gggatcatcg aggaattgcg gacccgggat ttcccgggcg
     1441 atcaactggc ggccgcccgg cgccgggtgg tcggaacccg gctcatcgga cgccagaccc
     1501 tttccagcca agccctcgat tacggtttga accagctgta cggactggac tgggattact
     1561 cggcgcgcct ggaatcgggc ctgcccggga tcgggaccgg tgaactggcc ggcttctgcc
     1621 gccgcttcct ggatccgaac cgcgccatcg tggtgaccac cgtgccgtca aaaacggaat
     1681 gaagaaacga ccgaaacttg atccgggggt catcctggcc ctgacggctc aggtgccgcc
     1741 gggccggttg acaacctacg gaacgctggc caggatcgcg ggccgcccgg gtggcgcccg
     1801 ggccgtcggc caggtactaa agcgcaaccc ggatcccgaa agggtcccct gttaccgggt
     1861 ggtccgctcc gacgggcggc tcggcggcta ccacgggacc tcggccgccg gtctcaagcg
     1921 gaaagcggcc cggcttcaag ccgatggaat cgagctgatt ccagggccgg aaccgcgcgt
     1981 ccggaacctg gaacgcttac ttttcaaggc cttccggacg cccggccggg gtcaaatcaa
     2041 gcggctttaa tttccggctg agttgtgcta taatttgctt ttaattttgc ccagatgcgc
     2101 tacctggcct gtgatcttgg tgcggaaagc ggccgaatcg tggccggaag cctgggaaaa
     2161 ggccggctga acctggaact cgtacatcga ttttccaacc agccggtctg gctgccgggc
     2221 ggattgcgct gggacacgct cggcatcttc cgggagatcg tctccggcct ggtccgttac
     2281 cggcaggccc accggaccga actggccggc ataagctgcg acagctgggg ggttgacttc
     2341 ggcctgctgg ataagcgcag ccagctggtc ggtaatccga accactaccg ctcccgccgt
     2401 ttcgagggag cgatggaaaa agtcctggca attctaccgg ccggtgaaat ttaccggcgg
     2461 accggcatcc agtttctccc ttttaatacc atttaccagc tttacacgct ggcgtccgag
     2521 tgcgatccgg cgctggagat cagtcgaaaa ctcctgatga ccgccgacct ggtgaacttc
     2581 tttctctccg gggaggccgc ccaggagttc acgctggcca ccaccagcca gctttacgat
     2641 ccgaaccgcc gcggctgggc ggccggactt ttcaggaaac ttgacctgcc gctcaagttg
     2701 atgccggcag tcaccccgcc ggccaccgtg atcgggaaac tgcgttggcg gatcggctct
     2761 gaaaccgggc tggcgggaac accggtaatc gcgggggcca gtcacgatac cgcggcggcc
     2821 gtggccgcct gtccggcccg gccgggagaa cggtttgcct acctttcctc cgggacctgg
     2881 tcgctgctgg gcgtggaact ggaacggccc ttgataaacg agacggtccg cgccgaaggt
     2941 ttcaccaacg aaggcggcct cggcggcact taccggttcc tgcacaacat caccggcctc
     3001 tggatcctgc aggaatgccg gcgggcctgg cagcatgaag gacgccttta cgagtacccg
     3061 gaattgaccg cgctggccgc cggggccagg ccgttccagg cgctcatcaa tcccgaccac
     3121 ccggatttcc tggccccgga gaagatgccg gaggccgtgg ccggcttctg ccggaaaacc
     3181 ggccagcgcc ccccgccgga ccggggcgga atggtccggg tcatcctgga gagcctggcc
     3241 ctgcgttacg gtcaattcgt caggaccctg aaccggctcg gggccctcgc cggaccgctg
     3301 gaggtgcttt acgtggtcgg cggcggcagt cagaacgagc ttctgaacca gttcaccgcc
     3361 aacgccaccg ggataccggt ggtgaccgga ccggtcgaag ctacggcggc cggcaatatc
     3421 ctggcccagg ccctggccgc cggcgaaata gcttcgatct gggaaggccg ggcgaccgtc
     3481 cgggccgcgc atgacctgaa aacttaccgg cccgacggac gggaacggtg gacggagatg
     3541 ctaaaccaga tggagaaggt atgttccatg gcttaacgac cagaactttt ccgggcgggg
     3601 tgcacccgcc ggaatcaaag tacacttcga cttcagccag cctcgaagcg ccgcggccgg
     3661 aaaaggccgt tctgatgctt tcccagcata ccggggcgcc ggcccgttcc ctggtcgccg
     3721 ccggggaccg ggtcgccacc ggccagaagg tgggcgaaaa aaccggcttc atctcggctt
     3781 cggtccattc ttcccttacc ggcgtggtca aggccgttga aatgcgcacc ggtccggccg
     3841 gaaaccggac cgaggccgtc gtggtggagg cttccggacc ggatgaattc cggtttctcg
     3901 aaccgcttga ctggaccacg gccacgcccg aagcgctccg ggaaagggta ggggaggccg
     3961 gcatcgtcgg actgggcggg gccgccttcc cgaccgaagt caagctcgcc ccgccccggc
     4021 cggtcgacac gctcctggtc aacggctgcg aatgcgaacc gtatcttacc gccgatcacc
     4081 ggctgatgct ggaaaaaccg gtcccggtgc tgaccggggc cctgttcagc gcccgcattc
     4141 tcaaggtgga ttcggtggtg gtcggcgtcg aagacaacaa gcctgacgcc ctcgagcagc
     4201 tctcccgggc cgcccgggaa ctggccggga acggaatcaa aatccgggtc gaggcggtcc
     4261 gcagcaagta cccccagggg gccgagaaga acctgatcca ggtcctgctc aagcgcctgg
     4321 taccacccgg caagctgccg atggaggtgg gcgcggtcgt ctccaacgtg gggaccgccc
     4381 tggccgtctg cgaggcggtg atgctgggca agccccttta cgaaagga
//
LOCUS       DAOQYN010000167         4378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_20431_length_4378_cov_4.228082, whole genome shotgun sequence.
ACCESSION   DAOQYN010000167 DAOQYN010000000
VERSION     DAOQYN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20431_length_4378_cov_4.228082"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PKN80_06895"
     CDS             <1..877
                     /locus_tag="PKN80_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35775.1"
                     /translation="ESYSTPPIPLSPAADSRSLFGVERRGIGYGQPLSHPLAEASRSD
                     LARYPWPDPDWLDTSRLHSQAAPYRETFAILGGAWSPFWHDLIDLLGLETLCLKMYED
                     PGFVDAVLDRITGFYLEVNRRIFEAAADRLDIFFIGNDFGSQTGPLISPNLFRRFLLP
                     RLEKFCVLGHRYGLKVMLHCCGGIYPLMPLLAESGLDAVHAIQTSCRGMELPRLKSEF
                     GNRILFNGCIDSHQVLIKGTPESIREDVRRVLEIMKPGGGFVAGASHDTILEETPLEN
                     TLAMFDAIREFGAYG"
     gene            complement(890..1660)
                     /locus_tag="PKN80_06900"
     CDS             complement(890..1660)
                     /locus_tag="PKN80_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35776.1"
                     /translation="MTGRLKRGVRSGGWFLLLAGLFWSAAVPVGAGPAGELEAIRLFD
                     EERLAAARPAGPEAAEAAFREVEKRYLDFLERYPRSADGRNYLGGLYYEHGWPELAAR
                     QWRLGLLANPRDPFLYNNLAEYFAHDRGQPARAIFLQEKAVRLKADEPVFHYNLATYY
                     DVFRFTARRRFGSLERVYESMLRSYRRALAFDPENYDYALVYAATLTLSPAAWKIADP
                     VAREEKLAAWRNCRRLAPDEPTRTMIDRQLRRIELQTP"
     gene            1818..>4378
                     /locus_tag="PKN80_06905"
     CDS             1818..>4378
                     /locus_tag="PKN80_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35777.1"
                     /translation="MKKNFFKKGIPGLAQIILLLAILGLAGAAPLRADEFFRRIWTDQ
                     QLPLPAGWFRHKEGHAFSMAAGTYVFPIGQTLPPGKYVIWLLPYYKDRQPLSIKLDDQ
                     FQPMQKRYFRGGWYGGNVILAGQPFSTITLDFSGVKNGINGIALSNNPDDGFLRGVIL
                     RAELAAQQSSAGVRNLFRGSSFETGAGYGYASNVKPADLTDGAWHGQVCLKTSLVSTR
                     WFGLSPGLPHILSFYVKTGAPASLEATVSGPRETEGASKSYGKRWEVSEAGKWQRYSL
                     EFTPEETLGNLYRVVIRTRDAQPFYVDAIQLEQGAVLSPYQPRSDLELGFRSRTLGNV
                     YRAGQSKGLDIYAFNNTAADTEAAFQLLTYDFYGRPAGRKSLTWKVPPGTHRFDYTEP
                     AAATGLFRAELRSAAGQLLDEIVYSVLPARPFAADVRYPEDGTLGTDSNPFLAEYLLA
                     ANRHWMINKHLGQLVFSTSYSFKFGVTPPDWEKPRPWLEITRESPVRVLATMGNYGAF
                     KVSDYGSMEGFLQAYGEHLLQTARFFKPGRVEHFEIWNEPYYTFKAEEYLELLKTGYR
                     AVKAGNPEAKVVGICGQTSWTGRLLELGAADYCDIISNHFYDNPRESMKEYGELVKKY
                     NKEAWNTETSGRCFSSYRSIPTLESVFADDPAAHFEEINRRENIPAALDRLKNYLYSI
                     TLARCRRYFTYVSRFNGDLADLHNTSSFGNTEWDGGWKATGVVQNIASFFLDGHTFRQ
                     EIPFAPDIESLHLVSRRNENLLVAWVRGPESYRLRLPSELKAGLHIHGMLGNEFPDPD
                     SMLLDQYPVYLRSNLEPAAFARLVGQIKVERIASPGPETNRKSCLVELESSAGAF"
BASE COUNT          869 a         1492 c         1216 g          801 t
ORIGIN      
        1 cgaatcctat tcgaccccgc cgattcccct ctccccggcc gccgactccc ggagcctctt
       61 cggcgtggaa cgccggggca tcggttacgg acagccgctc agccacccgc tcgcggaggc
      121 ctcccggtcc gacctcgcac gttacccctg gccggacccg gactggctgg acacttccag
      181 gcttcattcc caggcggcgc cctaccggga aacgttcgcc atcctgggcg gcgcctggtc
      241 ccctttctgg cacgacctca tcgacctgct gggcctggaa acgctctgtc tgaaaatgta
      301 cgaagacccc ggtttcgtcg acgcggtcct ggaccggatc accggtttct acctcgaggt
      361 gaaccgccgc atttttgaag ccgcggccga ccggcttgac atcttcttta tcggcaacga
      421 tttcggcagc cagaccgggc cgctgatcag cccgaacctc tttcgccgtt tcctgctgcc
      481 ccggctcgaa aaattctgcg tcctcggcca ccgctacggg ctgaaagtca tgctccattg
      541 ctgcggcggc atctatccgc tgatgccgtt gctggccgaa tccggtctcg acgccgtcca
      601 cgccattcaa acctcctgcc gcggcatgga gctccctcgt ttgaaatcgg agttcggaaa
      661 ccggatcctc ttcaacggat gcatcgattc ccaccaggta cttatcaaag gcacaccgga
      721 gagcatccgg gaggacgtgc gccgggtgtt ggaaatcatg aagccgggcg ggggattcgt
      781 cgccggggcc agccacgata ccattctgga agagaccccg ctggaaaaca ccctggccat
      841 gttcgacgcc atccgggagt ttggggccta cggttaattg cgaactctct cagggcgttt
      901 gcaattcgat ccgtcgcaac tggcggtcga tcatcgttcg cgtcggttcg tcaggggcca
      961 gacgccggca attcctccag gcggccagct tctcttcgcg cgcaaccgga tcggcaatct
     1021 tccaggcggc cggactcaag gtgagcgtgg cggcgtaaac cagggcataa tcgtaatttt
     1081 ccggatcgaa ggccagcgcc cgccggtaag agcgcagcat tgattcgtag accctctcca
     1141 gcgaaccgaa acggcgccgg gccgtaaaac gaaaaacatc gtaataggtg gccaggttgt
     1201 aatggaagac cggctcgtct gccttaagtc gaaccgcctt ctcctgcagg aaaatggccc
     1261 gggccggctg gccgcgatcg tgggcgaaat attcggccag gttgttgtag agaaacgggt
     1321 cgcgcggatt cgccagcagt ccgagccgcc actggcgagc cgccagctcc ggccagccgt
     1381 gttcgtaata aagcccgccc agataattcc gtccgtcggc actgcgcggg taacgttcga
     1441 gaaaatcaag atagcgtttc tcaacctccc ggaaggcggc ctcggccgcc tccggcccgg
     1501 ccggccgggc cgcagccagc cgttcctcgt caaacaggcg gatcgcctcc aattccccgg
     1561 ccggcccggc cccgaccgga accgccgccg accagaacaa cccggccaac agcaaaaacc
     1621 agccgccgga gcgcaccccc cgttttaatc ttccggtcat tccgttcccc caatttgaat
     1681 tttcatccag agtatactcc gcctccgatt ccccgggcaa acccggccgg cggcggccga
     1741 cttatctgat ttaagtgaaa catgataaaa taccctgtaa catttctgat ggtaacctga
     1801 acaaaaaagg cggccgcatg aagaagaact ttttcaaaaa aggcatcccg ggtctggcgc
     1861 agataatcct gcttctggct atcctcggcc tggccggggc cgccccgctg cgggccgatg
     1921 agtttttccg ccgaatctgg accgaccaac agctgccttt accggccggc tggttccgcc
     1981 ataaggaggg gcacgccttc tcgatggctg cgggaaccta cgtctttccg atcggccaga
     2041 cccttccgcc cggcaaatac gtaatctggc tgctgcctta ttacaaggac cgccagccgc
     2101 tttccatcaa gctggacgac cagtttcaac cgatgcaaaa acggtacttc cggggcggct
     2161 ggtacggcgg caacgtcatt ctggccggcc agcccttttc caccataacc cttgattttt
     2221 caggggtgaa gaacggcatc aatggcatcg ccctcagcaa caaccccgac gacggtttcc
     2281 tgcgcggagt gattctccgg gcggaactgg ccgcgcagca gagttcggcc ggcgtccgaa
     2341 acctcttccg cggcagcagt ttcgagaccg gcgccggtta cggatacgcc agcaacgtca
     2401 agcccgccga cctgaccgac ggcgcctggc acggacaggt ctgccttaaa accagtctcg
     2461 tatccacccg ctggttcggt ttgagcccgg gacttcctca catcctgagt ttttacgtaa
     2521 aaaccggggc tccggccagc ctggaggcaa ccgtctccgg cccccgcgaa acggagggtg
     2581 cgtccaagtc ctacggaaag cgctgggagg tatccgaagc cggcaaatgg cagcgttaca
     2641 gcctggagtt cacgcccgaa gaaaccctgg gcaacctcta ccgggttgtc atccggaccc
     2701 gggatgcgca acctttttac gtggacgcca ttcagctcga acagggcgcc gtcctgagcc
     2761 cctaccagcc ccgttccgac ctggaactcg gcttccggag ccggacgctt ggaaacgtct
     2821 accgggccgg ccagtccaag ggactggaca tttacgcctt caacaacacc gcggctgaca
     2881 ccgaagccgc cttccagcta ctgacctacg atttttacgg ccggccggcc ggccgtaaaa
     2941 gtctaacctg gaaggtgccg cccggcaccc accgcttcga ttacacggag ccggccgccg
     3001 ccaccggcct cttccgggcc gaactgcgct ccgccgccgg tcaactcctg gacgaaatcg
     3061 tctattcggt cctgccggcc cggcccttcg ccgccgacgt ccgctacccg gaagacggca
     3121 ccctgggtac cgactccaac cccttcctgg ccgagtacct gctggcggcc aatcgccact
     3181 ggatgatcaa caagcatctt ggccagctgg tcttctccac ctcctacagc ttcaagttcg
     3241 gcgttacgcc tccggattgg gagaaacccc ggccctggct ggagatcacc cgtgaaagcc
     3301 cggtccgggt gctggccacc atgggcaatt acggggcctt caaggtaagt gactatggtt
     3361 ccatggaagg ttttctccag gcctacggcg aacacctgct ccagacggcc cggttcttca
     3421 agcccggccg ggtcgagcac ttcgagatct ggaacgaacc ttactacacc ttcaaagccg
     3481 aggagtacct ggaactcctc aagaccgggt accgggccgt caaggctggc aacccggagg
     3541 cgaaggtggt cggtatctgc ggccagacct cgtggaccgg acgcctcctg gaactgggcg
     3601 cggccgatta ctgcgacatc atttccaacc acttctacga caacccgcgc gaaagcatga
     3661 aggaatacgg cgagctggta aaaaaataca acaaagaagc ctggaacacc gagaccagcg
     3721 gccgctgctt ctcgagctac cggtccatac ccacgctcga atcggtcttt gccgacgacc
     3781 cggccgccca tttcgaggag atcaaccgga gagaaaacat cccggcggcc ctggatcggc
     3841 tgaaaaacta cctgtattcg ataacgctgg cccgctgccg ccgctacttc acctatgtct
     3901 cgcgcttcaa cggggacctg gccgaccttc acaacaccag ttccttcgga aataccgagt
     3961 gggacggcgg ctggaaggca accggggtgg tccagaacat cgcctccttc ttcctcgacg
     4021 gccacacctt ccgccaggaa attcccttcg cccccgatat cgaatccctc cacctggtct
     4081 cgcgccgcaa cgaaaacctg ctggtggcct gggtgcgcgg accggaaagc taccgactcc
     4141 gactgccctc cgaactgaaa gccggactcc acattcatgg catgctcggc aatgaatttc
     4201 cggatcccga ttcgatgctc ctggaccagt atccggtcta cctccgttcc aacctggaac
     4261 cggccgcctt cgcccggctg gtcggccaga tcaaggtcga acggatcgcg tcgcccggac
     4321 cggaaacgaa ccggaagagc tgcctggtcg aactggaaag ctccgccggc gccttcga
//
LOCUS       DAOQYN010000168         4375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_20460_length_4375_cov_4.982870, whole genome shotgun sequence.
ACCESSION   DAOQYN010000168 DAOQYN010000000
VERSION     DAOQYN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20460_length_4375_cov_4.982870"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="PKN80_06910"
     CDS             <1..1126
                     /locus_tag="PKN80_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HNQ35778.1"
                     /translation="AVIRASTRVEDYAHLPLPVLFYARDSERITLRGPGRVDGQATGQ
                     VPWPRPKFRRRTLYFDSCRSVRVEEAAFENSDSWTLHFFRCERVEVRKVEILNDPEHI
                     NSDGIDPDSSRDVIISDCHIRAGDDCVVLKSNSPEHPCENIVVTGCRLETTCSALKLG
                     TESRGAIRQVSFNNCVITNTPLALACFMKDGGCYEDVLFEDIRIETADRLGHPEWPIF
                     VDLNLRTETSAPGRIRNLRFRNLEIVSSGTCLIQGLRRRPVEALRLQDILFRAAGPVD
                     FSRRLKMNGGRQEAHSQLEDYARTPAYFILANVDDFGLENLRLESVGPELPAGLHDLA
                     LLNCRCGRVAGLENRIRFTGPRLDPVLTEETPAGSVEFPG"
     gene            1279..2052
                     /gene="budA"
                     /locus_tag="PKN80_06915"
     CDS             1279..2052
                     /gene="budA"
                     /locus_tag="PKN80_06915"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNQ35779.1"
                     /translation="MKIRLAATILMVLFFGAGCAGLAPNRELLYQTSTLDALLEGDYQ
                     GRLTIGQLKRHGDFGIGTLADLDGELAGLDGRFYQVRADGKVFLVPDEGSTPFAAVTF
                     FEADRRLDLTGPLDYEGLKIRLDREIKRDLFGAVKISGRFRRVKTRSVPKQARPYPPL
                     AAVTDRQPVFEFQDLEGVLVGFYCPEFVGTVNAAGYHLHFLAADLSGGGHLLDCELVE
                     GTLEIDYTPDLYLALPGETAAGRGLNPAEKKRELERVEK"
     gene            2105..3082
                     /locus_tag="PKN80_06920"
     CDS             2105..3082
                     /locus_tag="PKN80_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNQ35780.1"
                     /translation="MEKRRLGWTELELTSVGLGTWAIGGPGLKFSWGPQDDRESVRTI
                     RHALELGINWIDTAAVYGLGHSETVVGQALRGLDEKPIVATKCCRVSDGAGGATFRFD
                     RASVRAEAEASLKRLGVERIDLYQIHHPGEGDSFLEAWETIADLVREGKVRYGGVSNF
                     SREQVERALKIHPVASLQPPYSLLRRDIESGLLEFCGANRIGVVAYSPMQKGILTDKF
                     TRDWVAGLPADDHRRNDPRFQGLALEVNLDFVARLREIAAAGNLTIGQLAIAWVLRRT
                     EVTAAIVGARHPYQIEETVQASGRALTPREIEAVGRLLSEFESDERLKV"
     gene            3092..3559
                     /locus_tag="PKN80_06925"
     CDS             3092..3559
                     /locus_tag="PKN80_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ35781.1"
                     /translation="MEIRGALPSDVPAILEMTAAAWAGVTIAGRLEERYGKIEGRPWH
                     EYKRGDLERFCVENLDRVLVALEGDRLVGYATYSLDPERRFGTVQNNAVHPDFRGRGI
                     GSALHRRVLDALRAAGMTRAFVTTLEVDEPAQRMYTRHGFRELARSIHYSLEL"
     gene            3584..>4375
                     /locus_tag="PKN80_06930"
     CDS             3584..>4375
                     /locus_tag="PKN80_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35782.1"
                     /translation="MFQTKSSRPSWKMAALLFLLIGMAVSLEAGGEPASFSRLYKSGD
                     LPLQPGWSAYAGLENVWPAIRCVFKAGAESPRFLDYRLPFDPPLVPGKYRVYLRNLNQ
                     GKVEVILGGRTELSHPLRNTWSAPVVFQLEQPASELIIRYHASKMTARNYFLQGVFIT
                     NRAEMVAPDDQVYPLAEWYGPPAPGWLRASEQLAIPLEWWAPSAELRKLFSVETHVPE
                     SDLAEVRAAGRKVVPYLDNPAHFGIDFHELERWAADPKDTRRGRWG"
BASE COUNT          731 a         1405 c         1448 g          791 t
ORIGIN      
        1 cgcggtcatc cgggccagta cccgggtcga ggattatgcc cacctgccgc ttccggtcct
       61 cttctacgcc cgcgattccg agcggattac cctccggggg ccgggccggg tcgacggcca
      121 ggcgaccggc caagtcccct ggccgcggcc gaagttccgc cgccgcaccc tctacttcga
      181 ttcctgccgg tccgtccggg tcgaagaagc cgccttcgaa aactctgatt cctggacgct
      241 gcactttttc cgctgcgagc gggttgaagt ccggaaagtc gaaattctga acgatcccga
      301 gcatatcaac tccgacggca tcgacccgga cagctcccgc gacgtcatca tttccgactg
      361 tcatatccgg gccggcgacg actgcgtcgt ccttaaatcc aacagcccgg agcatccctg
      421 tgaaaacatc gtggtgaccg gctgccggct ggagaccacc tgttcggcgc tgaaactggg
      481 caccgagagc cggggggcca tccggcaggt ctccttcaac aactgcgtga ttaccaacac
      541 gccgctggcc ctggcctgct tcatgaagga cggcggctgt tacgaggacg tgctcttcga
      601 agacatccgg atcgaaaccg ccgaccggct cggccatccg gaatggccga tcttcgtcga
      661 ccttaacctg cggaccgaaa cctcggcgcc gggccggata cgcaacctac ggttccgcaa
      721 cctggagatc gtcagttccg ggacctgcct catccagggg ctgcgccgcc gcccggtcga
      781 ggcgctgcgc ctccaggaca tccttttccg ggccgccggt ccggtggatt tttcccgccg
      841 tctcaagatg aatggcggcc gccaggaggc ccattcccag cttgaggatt acgcccggac
      901 ccccgcctac ttcatcctgg ccaacgttga tgacttcggt ctggagaatc tccggctgga
      961 atcggtcggc ccggagctgc cggccggcct gcacgacctg gccctgctga actgccggtg
     1021 cggccgggtg gccggcctgg agaaccggat ccgattcacc gggccgcggc tggatccggt
     1081 cctgacggag gagacgccgg ccggatcggt tgaatttccc gggtaatcga atggccggtt
     1141 ccggggctga aaaaagggcc gccggcgtcc ggggcacgcc tgattttgct ttcattatct
     1201 tttaagttat actgattgaa aagacattcc gggtgttttt tgtgcaagcc gaaaaacttg
     1261 ccgattcgaa ggagcgacat gaaaatccgt ttggccgcga caatcctgat ggtgcttttt
     1321 ttcggtgccg gctgcgccgg gctggcgccc aaccgggaac tcctgtacca gacctcgacg
     1381 ctggatgccc tgcttgaggg tgactaccag gggcggctga ccatcggcca gttgaagcgc
     1441 cacggcgatt tcggcattgg caccctggcc gatctggacg gcgaactggc cgggctggac
     1501 ggccgcttct accaggtgcg ggccgacgga aaggtcttcc tggtgccgga cgaaggcagc
     1561 actcccttcg cggccgttac ctttttcgag gccgaccgcc gtcttgacct gaccggtccg
     1621 ctcgattacg aggggctgaa aatccggctc gaccgcgaga taaagaggga cctttttggc
     1681 gcggtcaaga tatccggccg tttccgccgg gtgaagaccc gcagcgtgcc gaaacaggcg
     1741 cgcccttacc cgcccctggc cgcggtcacc gaccgtcagc cggttttcga gttccaggac
     1801 ctggagggcg tgctggtcgg cttctactgc ccggaattcg tcggcaccgt caacgcggcc
     1861 ggttatcacc tccattttct ggctgctgat ctttccggcg gcggccacct gcttgactgc
     1921 gaattggtcg aaggaacgct ggagattgat tataccccgg acctttacct ggcgttgccg
     1981 ggcgagaccg cggccggccg cggcttgaac ccggccgaga agaagcgcga gctggagcgg
     2041 gttgaaaagt gaccccgggg ggttcccggc cgggctgggc cttgaggtga aaaggagaag
     2101 aggcatggag aaacgaaggt tgggttggac cgaattggag ttgacctcgg tcggactggg
     2161 gacctgggcg atcggcggcc cgggcttgaa gttcagctgg ggcccccagg atgaccggga
     2221 gtcggtccga accatccgcc acgccctgga gctgggcatc aactggattg ataccgccgc
     2281 cgtttacggt ctgggccact cggagacggt ggtcgggcaa gcgctccggg gcctggacga
     2341 aaaaccgatc gttgccacca agtgctgccg ggtcagcgac ggcgccggcg gcgcgacctt
     2401 ccgttttgac cgggcttcgg tccgggccga ggccgaggcg agcctgaagc ggctgggggt
     2461 ggagcggatc gatctttacc agattcacca tcccggcgag ggtgactcct tcctggaggc
     2521 ctgggagacg atcgccgacc tggtgcgcga agggaaggtt cgctacggcg gagtctcgaa
     2581 tttcagccgg gagcaggttg agcgggccct gaagatccat ccggtcgcct cgctgcagcc
     2641 gccttacagc ctgctgcgcc gcgacatcga gtccggactg ctggaattct gcggcgcgaa
     2701 ccggatcggg gtggtcgctt acagcccgat gcagaagggc atcctgaccg acaagttcac
     2761 ccgggactgg gtggccggcc tgccggccga cgaccaccgc cggaacgacc cccgcttcca
     2821 gggattggcc ctggaggtca acctggactt cgtggcccgg ttgcgggaga tcgccgcggc
     2881 cgggaacctg acgatcggcc agctggccat cgcctgggtc ctgcggcgga ccgaggtgac
     2941 cgccgccatc gtcggcgccc gccatcctta ccagatcgag gagaccgtcc aggcctcggg
     3001 ccgcgctttg accccccggg aaatcgaagc cgtcggccgc ctgctctcgg agttcgagtc
     3061 cgacgaacgc ttgaaggttt gaggagacgg catggaaata agaggagcac tcccgtctga
     3121 tgtcccggcc atactggaga tgaccgcggc cgcctgggcc ggggtgacga tcgccggccg
     3181 gctggaggag cgttacggga agatcgaggg gcgtccctgg cacgaataca aacggggcga
     3241 cctggagagg ttctgcgtcg agaacctcga ccgggtcctg gtggcgctgg aaggggaccg
     3301 gttggtcggc tatgccacct acagccttga cccggaacgg cggttcggga cggtccagaa
     3361 caatgccgtt cacccggatt tccgggggcg gggcatcggg tcggccctgc accggcgggt
     3421 gctggatgcc ttgcgggcgg ccggcatgac cagggccttc gtcacgacgc tcgaggtgga
     3481 tgaaccggcc cagcgcatgt acacccggca cgggttcagg gaactggccc gttcgatcca
     3541 ctactcgctc gagctttaaa aatacagccg aggagacaac ctggtgttcc aaacaaaatc
     3601 gtcccggccg tcctggaaga tggcggcact cctgtttctt cttatcggga tggccgtttc
     3661 actcgaagcc ggcggcgaac cggcgtcctt ctcccgcctc tacaagtccg gcgatctgcc
     3721 gcttcagccg ggctggtcgg cctatgccgg cctggagaac gtctggccgg ccatccgctg
     3781 cgttttcaag gcgggcgcgg aatcgccccg tttcctggac taccgtcttc cctttgaccc
     3841 gcccctggtt cccggtaagt accgggtcta cctgcgcaac ctgaaccagg gcaaggtcga
     3901 ggtgatcctg ggtggccgga ccgagctgtc ccacccgctc cggaacacct ggtccgctcc
     3961 ggtggttttc cagctcgagc agccggcctc ggagctgatc atccgctacc acgcttcgaa
     4021 gatgaccgca cggaactact ttctccaggg ggtcttcatc accaaccggg ccgagatggt
     4081 tgctcccgac gaccaggtct atcctctggc cgagtggtac ggtccgccgg cgcccggctg
     4141 gctccgggcg agcgaacagc tggcgatacc cctggaatgg tgggcgccgt cggccgagct
     4201 gcgcaagctc ttttcggtgg agacccatgt gccggagtcg gacctggccg aggtgcgcgc
     4261 ggccggccgg aaggtggttc cctacctgga taacccggcc catttcggga tcgatttcca
     4321 cgagctggaa cgctgggcgg ccgatcccaa ggatacgcgc cgggggcgct ggggc
//
LOCUS       DAOQYN010000169         4339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_20684_length_4339_cov_4.310224, whole genome shotgun sequence.
ACCESSION   DAOQYN010000169 DAOQYN010000000
VERSION     DAOQYN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20684_length_4339_cov_4.310224"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PKN80_06935"
     CDS             complement(<1..782)
                     /locus_tag="PKN80_06935"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HNQ35783.1"
                     /translation="MKICDREWKREELLKRVGDISQLAGIRRLELAEGNEKGVEAAEL
                     RSGSGLRAVILLSRAMDLGFVEYAGRPLCWRSPVGDAHPAFYEPQEDGWLRTFYGGMM
                     NTCGLTYFGAPCDDAGKRLGVHGRASHLPARLLASEGAWQDGEYRLTLKGRIRESTLF
                     GENVSLTRTYTMTAGEDRILLHDVVENEGYRTTEHMILYHVNVGFPVLDEGSELLLPV
                     RSTRPLTEHAAQGLADYNRFGTPVAGFQEQVYLHRLAADALLV"
     gene            complement(854..2140)
                     /locus_tag="PKN80_06940"
     CDS             complement(854..2140)
                     /locus_tag="PKN80_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNQ35784.1"
                     /translation="MKRRGWARLVVPGLLILAGTAGVLAAQEAKTAARPEWNGEELEF
                     FYQNTYDNSSKPAILYKPAGIEKVAKAPLLIKLHYMSGDRFTVRRQGYYQEADKRLWY
                     VLSPELHGKNTPGGRTSWASLEAQHDVIDALETVLRNTPNIDRRRIYLAGRSMGGTLA
                     LLMAAKYPDRFAAVVAGQPVTDYESYYRTSVFIRKSPVKEVFLKEMGGEPKEVPFEYR
                     RRSSFSYARNLACLPVTIWAGVEDSVVPREQVELLHREMSIYNPYQPPVNWLVGAPHN
                     AGNYNAAWVCDHLQYYENIAENEMKRLPYRYYRSLDLRTDESKPFFYLNPVLAREDDL
                     GEMRSSIKDGKLAVVTRNLKELTIDLYQAAGLPADRAAAAAIVSRYEVRSDRSLKFIM
                     LYDGQPLREESVVNLKSGEIPWGGEDPGLRNNRIKQ"
     gene            complement(2169..2957)
                     /locus_tag="PKN80_06945"
     CDS             complement(2169..2957)
                     /locus_tag="PKN80_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005986004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ35785.1"
                     /translation="MSSGLGNISWRAVRVWQRNWTVYCATWLINFLAPILEPLLFLLA
                     FGVGLGRLVPSVAYGGLEIPYRVFIMPGILAVSVMNYAFFETTYASFVRIYFQKTYDA
                     ILATPLLLEDVLAGEILWGATKSVLATAIMMVVVSCFGLLVYPAALWLIPAALLGGLA
                     FASLGMIFTGLVGSIDQFNLPIFLLVTPMYLFGGVFFPLESLPDWAVNLAKLLPLTHL
                     VIVMRQLALGRVDASLAGSLAALLVFGAACFVLALYLLRRRLIK"
     gene            complement(2954..3871)
                     /locus_tag="PKN80_06950"
     CDS             complement(2954..3871)
                     /locus_tag="PKN80_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ35786.1"
                     /translation="MSAANVVYAEGLTKKFDGLAAVAGIDFQVGRGECFGLLGPNGAG
                     KTTTVRMAACFLPPDGGRLEVFGLPVEAHPRRIKARLGVCSQEDNLDPDLNVLQNLLV
                     YSRYFDIDRRTARVRAEELLGFVGLAGRRASPVLQLSGGMKRRLVIARSLINRPELLI
                     LDEPTTGLDPQARHQVWDYILALKAGGTTSLLTTHYMEEAAALCDRIIIMDRGRIVIE
                     GRPAELVRSVVGERVVEITGYDEAEERFLRERQLYFEKTARRLLVFSGRSEESFAEIT
                     GRFCRGGCTLRMANLEDVFLKLTGRELKE"
     gene            complement(4012..>4339)
                     /locus_tag="PKN80_06955"
     CDS             complement(4012..>4339)
                     /locus_tag="PKN80_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35787.1"
                     /translation="PVSGREEVGVFSFPESGGRPAGLLWREEHGRLWKRLGVNAVQYQ
                     TCRGIQLAVQLLQETRLGRRPGTWTAFSLPLSAADWRDIAQREARLGIKWLPASGLRI
                     GRPPGG"
BASE COUNT          795 a         1410 c         1437 g          697 t
ORIGIN      
        1 acgagcagcg cgtcggcggc cagccggtgc agatagacct gctcctggaa accggcgacc
       61 ggcgtgccga aacgattgta atcggccagt ccctgggcgg cgtgctcagt gagcggccgg
      121 gtcgaacgca cgggcaaaag caattcgctc ccctcgtcga gcaccgggaa tcctacgttg
      181 acgtggtaga gaatcatgtg ctcggtggtc cggtagccct cgttttcgac cacgtcgtgg
      241 agaaggatcc ggtcttcgcc ggcggtcatg gtataggtgc gggtaaggga aacgttctcg
      301 ccgaagaggg ttgactcccg gatgcgccct ttcagggtga ggcggtactc cccgtcctgc
      361 caggcgccct cgctggccag gagccgggcc ggcaggtggg aagcccggcc gtgcactccc
      421 agccgcttcc cggcatcgtc gcagggcgcg ccgaaatagg tcaggccgca ggtgttcatc
      481 atcccgccgt agaaggtgcg cagccagccg tcctcctgcg gctcgtagaa ggccgggtgg
      541 gcgtcgccca ccggcgaacg ccagcagagc ggccggccgg cgtattcgac gaagcccagg
      601 tccatcgccc ggctcaagag aatcaccgcc cgcaacccgc tgccggaacg cagttcggca
      661 gcctcgacgc ccttctcgtt tccctcggcc agctccaggc gcctgatccc ggccagctgg
      721 gaaatgtcgc ccacccgttt caaaagctct tcacgcttcc attcccggtc gcagattttc
      781 atgacgacac ctccaggcca acccggccgg cgcgacggaa tcgctgatcc cgcgcgcctt
      841 ccggcggaac cagttactgt tttattctgt tgttacggag accgggatct tcgccacccc
      901 aggggatctc tccgctcttg agattgacca ccgactcctc gcgcaggggc tggccgtcgt
      961 agagcatgat aaacttcagg ctccggtcgc tccgaacctc gtaacggctg acgatggcgg
     1021 ccgcggcggc ccggtcggcc ggcagcccgg cggcctggta gagatcgatg gtcagttcct
     1081 tgaggttgcg ggtgacgacc gccagcttgc cgtccttgat cgaactccgc atctcgccca
     1141 gatcgtcctc ccgggccaga accggattga ggtagaagaa gggcttcgac tcgtccgtgc
     1201 gcaggtcaag ggaccggtag tagcggtaag gcagccgctt catctcgttc tcggcgatgt
     1261 tttcataata ctgaagatga tcgcagaccc aggcggcgtt atagttgccg gcgttgtgcg
     1321 gagcgcccac cagccagttg accggcggct ggtaagggtt gtagatcgac atctcccggt
     1381 gcagcaactc cacctgctcg cgcggcacca cggaatcctc gaccccggcc cagatggtca
     1441 ccggcaggca ggccaggttg cgggcgtagg agaaggagga acggcggcgg tactcgaagg
     1501 ggacctcttt tggttcaccg cccatctcct tgagaaaaac ctccttgacg ggcgacttgc
     1561 ggatgaaaac ggaggtccgg tagtaactct cgtagtcggt caccggctgg ccggccacca
     1621 cggccgcgaa gcggtccggg tacttggcgg ccatcagcag ggccagcgtg ccgcccatcg
     1681 accggccggc caggtagatg cgccgccggt cgatgttggg ggtgttgcgt aggaccgttt
     1741 ccagggcgtc gatcacgtcg tgctgcgcct cgagcgaagc ccaggaggtc cggccgccgg
     1801 gagtgttctt gccgtgcagc tcgggcgaaa gcacgtacca gagacgtttg tcggcctcct
     1861 ggtagtagcc ctggcgccgg acggtgaacc ggtcgccgct catgtaatgg agcttaatca
     1921 gcaggggcgc cttcgccacc ttctcgatgc cggccggctt gtagagaatg gccggcttgc
     1981 tgctgttgtc gtaggtgttc tggtagaaga attccagttc ctcgccgttc cactcgggcc
     2041 gggcggccgt tttggcctcc tgggccgcca gcaccccggc ggtgccggcc agaatgagca
     2101 accccggaac caccagcctg gcccatcctc ttcgcttcat aagaaccctc ctcttggctt
     2161 gtgaaagttt acttgatcag gcggcggcgc agcaggtaaa gcgcgagcac gaaacaagcg
     2221 gcgccgaaaa ccagcagggc ggccaggctc ccggccagcg aggcatccac ccggcccagg
     2281 gccagctggc gcatgacgat gaccaggtgg gtcaggggca gcagcttcgc caggttcacc
     2341 gcccaatccg gcagggattc cagcggaaag aagaccccgc cgaagagata catcggcgtc
     2401 accagcagaa agatgggaag gttgaactgg tcgatgctgc ccaccaggcc ggtgaagatc
     2461 atgcccagcg aggcgaaggc caatccgccc agcagggcgg ccggtatcag ccagagagcg
     2521 gccggataga ccagcagccc gaaacaggag acgacaacca tcatgatggc cgtggccagg
     2581 accgacttgg tggcccccca gagtatctcg ccggccagca cgtcctccag caggagcggc
     2641 gtggccagga tggcgtcgta ggtcttctgg aagtagatgc gcacgaagga ggcgtaggtc
     2701 gtttcgaaga aggcgtaatt catgaccgag acggccagga tgccgggcat gatgaagacc
     2761 cggtagggaa tttccaggcc gccgtaggca accgagggca ccagccgccc caggccgacc
     2821 ccgaaggcca gcagaaagag cagcggctcc aggatcgggg ccagaaagtt gatcagccag
     2881 gtggcgcagt aaaccgtcca gttgcgctgc cagacccgga ccgcccgcca gctgatgttg
     2941 cccagtcccg aactcattcc ttcagctccc ggccggtcag tttcaaaaag acatcctcga
     3001 ggttggccat gcgcagcgtg cagccgcccc ggcagaagcg gccggtgatc tcggcaaacg
     3061 attcctcgct ccggcccgaa aagaccagca gccggcgggc ggtcttctcg aagtagagct
     3121 ggcgttcccg aaggaaccgc tcctcggcct cgtcgtaacc ggtgatctcc accacccgct
     3181 cgcccaccac cgaccggacc agctcggccg gccgcccctc gatcacgatg cgcccccggt
     3241 ccatgatgat gatccgatcg cagagcgcgg ccgcctcctc catgtagtgg gtggtcagca
     3301 ggctggtggt gccgccggcc ttgagggcca ggatgtaatc ccagacctgg tggcgcgcct
     3361 gcgggtcgag gccggtggtc ggctcgtcca ggatgagcag ctccggccgg ttgatcaggg
     3421 aacgggcgat gaccaggcgg cgcttcatgc cgccggagag ctggaggacc gggctggcgc
     3481 ggcggccggc cagtccgacg aagccgagca gttcttcggc ccggacccgg gcggtgcgcc
     3541 ggtcgatatc gaagtagcgg gagtaaacca gcaggttctg gagaacgttc aggtccggat
     3601 cgaggttgtc ctcctgggag cagacgccga gccgggcctt gatccggcgc gggtgtgcct
     3661 cgaccggcag gccgaagacc tccaggcggc cgccgtcggg cggcaggaag caggcggcca
     3721 tccggaccgt ggtggtcttg ccggcgccgt tgggccccag gagaccgaag cactcgccgc
     3781 ggccgacctg gaagtcaatc ccggccaccg cggccaggcc gtcgaacttc ttggtcaacc
     3841 cctcggcgta aacgacattt gcggcagaca ttttttatgt tttaaaagac ggttcttaag
     3901 agacagccca agcgaggaaa gggatgaaca ccttccggag agattttaac actaaagcgg
     3961 ccggctgtca ggcgcggccg gtccggagcc ggagccggaa aggccggccg ttcaaccgcc
     4021 cggcggccgg cctatccgca gcccggaagc cggaagccat ttgatgccca ggcgggcctc
     4081 ccgttgcgcg atgtcccgcc agtcggcggc cgaaaggggc aacgagaagg cggtccaggt
     4141 gccgggccgt cgacccagcc gggtctcctg gaggagctgg acggccagtt gtatcccgcg
     4201 gcaggtctgg tattggaccg cgttcacccc aagccgtttc cagaggcggc cgtgctcctc
     4261 ccgccagagc agcccggccg gccggccgcc cgactccgga aaagaaaaaa ctccgacctc
     4321 ctcccgtccg gaaaccgga
//
LOCUS       DAOQYN010000170         4323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_20775_length_4323_cov_3.828960, whole genome shotgun sequence.
ACCESSION   DAOQYN010000170 DAOQYN010000000
VERSION     DAOQYN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20775_length_4323_cov_3.828960"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2489)
                     /locus_tag="PKN80_06960"
     CDS             complement(<1..2489)
                     /locus_tag="PKN80_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNQ35788.1"
                     /translation="MKSRQSKKTGTISVILAAGEGSRMESPHLHKVCFPIEGRPAIVR
                     AIETYQQCGIQTHLLVVGNRAEQVMAAAAGAPGVHLFCHQPRPTGTGNAARMAAELLA
                     SLDYRDNILLVAGDKVIESEPLQRLQTVFEESGADLAFLAGEIQHFPDSGRIIYGRDN
                     RVRGILEVFDIARVQLLQALERICRERPLTAQEVQAMALGYFRRETKAALALGGLWDA
                     VRSGQPITRNLLKRNFRPGDFEFQVNRRRLEPELVSGLKHVNLSVYLFRAPALYQALE
                     QISRDNAQQEEYLTDTVGVLADAGARLEAVPVVNPHQVMAYNTPAELEKIEAYLASRR
                     RISVREGRKALRPATEWLAMVERPGRELNAALAEIYGSDFPYQEQKRGQLAALLRRYL
                     ELFGDEPVVIGRAPGRLNIMGRHIDHQGGHINLIATNRDVFVAAGIRPDRKIRLVNLH
                     PQLFADRAFAIEEIPDGCREKNWLDFVNGPKVTQNLARNGGDWSQYIKAVLARFQARF
                     PDRRLAGMNLAVTGDVPIASGLSSSSALLVAAAEAVAALNRLNLAAEEFVELCGEGEW
                     FVGTRGGSGDHAAMKFARRGQVVQVGFFPLQITDTVPFPEEHCLLIANSHQPARKTAG
                     VRDIFNHRIACYQIGRELLKQAFPHYAPSIEHLRDFNTERLAVDYPTLFRMLKAIPTE
                     ITRNEARKHVTDARALACLESHAEHFNRYPVRAVVIFGLAECQRSYLGPNLMRRGQVE
                     EFGRWMSVSHDGDRVVSWNEEGLSRPFTVDYSDPALDRLIREARRDREESLLRRQPGS
                     FSCSTPQLDRLVDLARRQPGVKGAQLSGAGLG"
     gene            2607..3047
                     /gene="mscL"
                     /locus_tag="PKN80_06965"
     CDS             2607..3047
                     /gene="mscL"
                     /locus_tag="PKN80_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNQ35789.1"
                     /translation="MFKEFKEFAMRGNMLDMAIGIIIGAAFGKIITSLVNDLLMPPIG
                     LVMGKVNFADRFVNLSGQPYASLAEARAAGAATINYGIFLNTIIDFVIVAFVVFLLVR
                     QINRMKKAAASEPVTRACPYCCTEIPIKAVRCPHCTTELKVEQA"
     gene            3094..3306
                     /locus_tag="PKN80_06970"
     CDS             3094..3306
                     /locus_tag="PKN80_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35790.1"
                     /translation="MKKLWLLVCLVLLLAGCAAGRAVGRTLRPSNVVRVAEVCREGPS
                     AFRFLGAWKDRFSRFLRPGFSRIFGR"
     gene            complement(3353..4141)
                     /locus_tag="PKN80_06975"
     CDS             complement(3353..4141)
                     /locus_tag="PKN80_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35791.1"
                     /translation="MSRGTPDDYRDLPAPEMEARLARFEEAVNAAQRANPPTKERVRM
                     ALHGKGDGRCPVRLKRHSLDLILKYGDDLAALFCAYPDDAVAVIPYDITIGYQPPNKT
                     PRVNAVEALVLDRQWVDEWGTRWGHALCGVGATPLECPLQDWEMLDAYLATGVPEPGA
                     PGRFDAAASLLALHGEGKYCFGIIHLALFERLHALRGMENVLTDFVTHEPEVGRLLLE
                     GAPAEEIERAAVGQGMITFAADGMRRAAAGETTIEEVLLTLALK"
     gene            complement(4138..>4323)
                     /locus_tag="PKN80_06980"
     CDS             complement(4138..>4323)
                     /locus_tag="PKN80_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35792.1"
                     /translation="SVTIDNMTAYYAAGAFAGVAGVTTEMPLADAVRSSNFAEITACA
                     RQWVSAVRAMKAGNATA"
BASE COUNT          693 a         1415 c         1449 g          766 t
ORIGIN      
        1 ccgaggccgg ccccggaaag ctgggcgccc ttaacgccgg gctggcgccg ggccaggtcc
       61 accagccggt cgagttgcgg ggtgctgcag ctgaagctgc cgggctggcg ccgaaggagc
      121 gactcctccc ggtcccggcg cgcctcccgg atcagccggt ccagcgccgg gtcggaataa
      181 tcgacagtga aggggcggga gagtccctcc tcgttccagc tgaccacccg gtcgccgtcg
      241 tgggagaccg acatccagcg gccgaactcc tccacctggc cgcgccgcat cagattcggc
      301 ccgaggtaac tgcgctggca ttcggccagg ccgaagatta ccaccgcccg gaccgggtag
      361 cggttgaagt gttccgcgtg ggactcgagg caggcgagcg cccgggcgtc ggtgacgtgt
      421 ttccgggcct cgttccgggt gatctcggtg ggaatcgcct tgagcatccg gaagagggtc
      481 ggataatcca ccgccagccg ttcggtgttg aaatcgcgca ggtgttcgat gctcggtgcg
      541 taatgcggga aggcctgctt gaggagttcg cgcccgatct ggtagcaggc gatccggtga
      601 ttgaagatgt cccgtacccc ggcggtcttg cgcgccggct ggtgggaatt ggcgatcagg
      661 aggcagtgtt cctcgggaaa aggcacggta tcggttatct gaagcgggaa gaagcccacc
      721 tggactacct ggccgcggcg ggcgaacttc atggccgcat ggtctccgga gccgccgcgg
      781 gttcccacga accattcgcc ttccccgcag agttccacga actcctcggc cgcgaggttg
      841 agccggttga gggcggccac cgcctcggcg gccgccacca gcagggccga agaggagctc
      901 agtccggagg cgatcggcac gtcgccggtc accgccaggt tcatcccggc cagccgccgg
      961 tccgggaagc gcgcctggaa gcgggccagg acggccttga tgtactggga ccagtcgccg
     1021 ccgttgcgcg ccaggttctg ggtcaccttc gggccgttca cgaagtcgag ccagttcttc
     1081 tcgcggcagc cgtccggaat ctcctcgatg gcaaaggccc ggtccgcgaa gagctggggg
     1141 tgcagattga ccaggcggat tttccggtcc ggccggattc cggcggccac gaagacatcc
     1201 cggttggtgg cgatgagatt tatgtggccg ccctggtgat cgatgtggcg gcccatgatg
     1261 ttgagccgcc ccggcgcccg gccgatgacc accggttcgt cgccgaagag ttccaggtac
     1321 cggcggagca gggcggccag ctggccgcgt ttctgctcct ggtagggaaa atcgctgccg
     1381 tagatttccg ccagggccgc gttgagttcc cggcccggcc tttcgaccat cgccagccac
     1441 tcggtggctg gccggagcgc tttgcgcccc tcccggaccg agatccggcg gcgcgaagcc
     1501 aggtaggcct cgatcttctc cagttcggcg ggcgtattgt aggccatcac ctggtggggg
     1561 ttgaccaccg gcaccgcctc cagccgggcc ccggcgtcgg ccaggacgcc caccgtgtcg
     1621 gtcaggtact cctcctgctg ggcgttgtcc cgggagatct gctccagcgc ctggtaaagg
     1681 gccggcgccc ggaagaggta aacggagaga ttgacgtgtt tcagcccgga gaccagctcc
     1741 ggttccaggc ggcgccggtt gacctggaat tcgaagtccc cgggccggaa gttccttttc
     1801 agcaggttcc gggttatcgg ctggccgctt cggaccgcgt cccagagacc gcccagggcc
     1861 agggcggcct tggtctcccg gcggaagtag ccgagggcca tggcctggac ctcctgggcg
     1921 gtcagcggcc tttcccggca gatccgttcc agggcctgga gcagctggac ccgggcgatg
     1981 tcgaagacct ccaggatgcc ccggacccgg ttatcccgtc cgtagatgat ccggccggaa
     2041 tccgggaaat gctggatttc gcccgccagg aaggccaggt cggccccgct ttcctcgaag
     2101 acggtctgca ggcgctggag cggttccgat tcgatgacct tgtcgccggc caccagcagt
     2161 atgttgtctc ggtagtcaag ggaggccagg agttcggccg ccatccgggc ggcgttgccg
     2221 gttccggtgg gtcggggctg gtggcagaag agatggacgc ccggggcgcc ggcggccgcc
     2281 gccataacct gttcggcccg gtttccgacc accagcaggt gggtttggat accgcattgc
     2341 tggtaggttt cgatggcccg gacgatcgcc ggccgcccct cgatcgggaa acagaccttg
     2401 tgcaggtggg gcgactccat ccgggagccc tcgccggcgg ccaggatgac cgagatggtc
     2461 ccggtcttct tcgattggcg cgatttcatc ttcaattata ataaaatagc atggcgccgg
     2521 cgaaatgaca aatatcaacg aattgtgata agttataagg caaagacccc gcggcggggt
     2581 ggaacacttt gaaaggggag gggggaatgt tcaaggaatt caaggaattc gccatgcgcg
     2641 gcaacatgct tgacatggcc atcggcatca tcatcggcgc cgccttcggc aagatcatca
     2701 cctcgctggt caacgacctc ctgatgccgc cgattggcct ggtcatgggc aaggtcaact
     2761 tcgccgaccg ctttgtcaac ctctccggcc aaccgtacgc cagcctggcc gaggccaggg
     2821 cggccggcgc ggccaccatc aactacggca tcttcctcaa caccatcatt gatttcgtca
     2881 tcgtggcctt cgtcgtcttc ctgctcgtgc gccagatcaa ccggatgaaa aaggcggcgg
     2941 cgtcggaacc ggtcacccgg gcatgtccct actgctgcac cgagataccg atcaaggccg
     3001 tccggtgtcc ccactgcacc acggaactga aggttgaaca ggcctgatct cgttgaatac
     3061 agtgatgaag ggcgcggccg ggccggaggc gggatgaaga aactctggct gctggtctgc
     3121 ctggtcctgc tgctggccgg ttgcgccgcc ggtcgggccg tcggccgtac cctgcgcccc
     3181 tccaacgtgg tcagggtggc cgaggtctgc cgcgagggcc cgtcggcctt caggtttctg
     3241 ggcgcctgga aggaccgctt ctcacgcttc ctgcgcccag gcttcagccg gatcttcggc
     3301 cggtgaggaa gggccgggcc gataccggtt ccgggtaaat ctccggggtt ttctacttca
     3361 gcgccagggt gaggaggacc tcctcgatgg tcgtctcgcc cgccgccgcc cggcgcatcc
     3421 cgtcggccgc gaaggtgatc atgccctggc cgacggcggc ccgctctatc tcttccgccg
     3481 gggcgccctc cagaagcagc cggcccacct ccggttcgtg ggtcacgaaa tcggtcaaca
     3541 cgttttccat gccccggagg gcgtgaagcc gctcgaaaag ggcgagatga atgatcccga
     3601 aacagtactt gccctccccg tgaagcgcga gcagggaagc cgcggcgtcg aaccggccgg
     3661 gcgctcccgg ctcgggcacg cccgtcgcga gatacgcgtc cagcatctcc cagtcctgca
     3721 ggggacactc cagcggggtt gcgcccacac cgcagagggc atgcccccac cgcgtccccc
     3781 attcatcgac ccactgtctg tccagcacaa gcgcttccac ggcgttcaca cgcggggtct
     3841 tgttcggcgg ctggtacccg atcgtgatgt cgtaggggat gacggcaacc gcatcgtcgg
     3901 gatacgcgca aaaaagcgcg gccagatcgt cgccgtattt caggatcagg tccagcgagt
     3961 ggcgtttcag gcggacggga caacgcccat cgcctttccc gtgaagggcc atgcgcaccc
     4021 gttctttcgt cggcgggttg gcgcgctgcg ccgcgttcac ggcctcttcg aaccgggcga
     4081 ggcgggcttc catctcgggg gcgggcaggt cgcgataatc gtctggtgtc ccgcggctca
     4141 tgctgttgcg ttcccggcct tcatagccct caccgcggag acccattggc gcgcgcacgc
     4201 cgtaatctcc gcgaaattgc tgctccgcac ggcgtccgca agcggcattt ccgtcgtcac
     4261 gcccgccaca ccggcaaacg cccccgcagc gtagtaggcc gtcatgttgt cgatcgttac
     4321 act
//
LOCUS       DAOQYN010000171         4275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_21070_length_4275_cov_4.177014, whole genome shotgun sequence.
ACCESSION   DAOQYN010000171 DAOQYN010000000
VERSION     DAOQYN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21070_length_4275_cov_4.177014"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKN80_06985"
     CDS             complement(<1..214)
                     /locus_tag="PKN80_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNQ35793.1"
                     /translation="MAGERDFCVTDFGARGGGPAPEHPAFQRAIDACVRAGGGRVRVP
                     AGRYLCGGLTLGSGLELRLEAGAVIRA"
     gene            330..1073
                     /locus_tag="PKN80_06990"
     CDS             330..1073
                     /locus_tag="PKN80_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNQ35794.1"
                     /translation="MSELCVIILAAGKGTRMKSDLQKVLHQLLGKPMIHHLVEEVRRL
                     EPVKVLVVVGHQKEEVMQRLEGLPVEFVEQTEMRGTGDAVRRAVERLPGYAGEILVLC
                     GDSPLLRSETLLKLLELHRREGPAATLLTARLENPTGYGRIKRNGTGLVEAIVEQADA
                     TDEERRITEVNSGVYVFAAPRLFETLPGLRPDNAKGEYYLTDVIKFLRTAGERVAAQV
                     VADPSEILGVNTPEELERARQILARRRQS"
     gene            1070..2005
                     /locus_tag="PKN80_06995"
     CDS             1070..2005
                     /locus_tag="PKN80_06995"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNQ35795.1"
                     /translation="MNNCLLFTGSANQELGAAIAAYLDKQVGRMAITHFSDGEIMVAI
                     EENVRGKDVFIIQSTCPPVNENLMELLVMIDAFKRASAERITAVIPYFGYARQDRKDK
                     PRVPITAKLVANLLTASGVNRILTMDLHTPQIQGFFDIPVDHLFAAPVTVSYFQEKKL
                     DRMVVVSPDVGSVKMARAFAKALRTPLAIVDKRRDGPTSVEAVNLIGEIDGSVAILVD
                     DMISTGGTLMEAAEVLKSKGATAIYASCSHGVLVGDAVARLAASSIAELVITDTIPLA
                     PEKRLEKIKVLSVAGLLGEAIKRIHLSASVSSLFL"
     gene            complement(2078..3283)
                     /locus_tag="PKN80_07000"
     CDS             complement(2078..3283)
                     /locus_tag="PKN80_07000"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNQ35796.1"
                     /translation="MLDFERLQKYLDIPETARMMLSAPEKQVIMSVNIKVGAGKLLIY
                     DSYLVYHNLVLGPAKGGIRFAPNVTLAETRTLAELMTYKCALMGIPFGGGKSSIALNP
                     RSISEFEKRMLIREFVHMIRDELVSGAYIPAPDLGSTPSDMAIIYGETHHPESVTGKP
                     PRLGGLPGRREATGRGVFTASRLACREILNLDVSKASVAIQGCGNVGSHSAAFLHESG
                     ARVVALSDVSGGLYSKAGLPVPAILDFVRQGKLLEEFPAEGLERITNQAILALPVDLL
                     IPAAVEDVLTAETAPGVQAKLVVEAANGPTTPEGDQILARKKIPVISDIMANSGGVVA
                     SYVEWRNAKSGNQTSAQETYDFIDAKMEGAFKDVARRVREQKASWRDGAYLLALSELI
                     SAMRDRSWI"
     gene            complement(3389..>4275)
                     /locus_tag="PKN80_07005"
     CDS             complement(3389..>4275)
                     /locus_tag="PKN80_07005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ35797.1"
                     /translation="NHPSIIMWVPFNEGWGQYDTERIVGLVRIFPLDLRQGPVRLKAA
                     GIGGVATRPELRGRGFMTRLMERALERMRGDGYPVSVLWGDRFRYRRFGYEQAGRTLS
                     LRLTARSLKAAGVTPAPVRRYLGEPGWLDQVRAAYEKHPFRLRRRPGDYRLIFEKPGL
                     FLFGGGRGQDFGYLAVTGRGAWQPVEFGGRPETVLAVAAGVMERLGTDSLTFSMAAGS
                     DGIEAFQSVAAGWSLSSELMAAVLDLEKTLAIFGRQNPDFKPDPESLARLDPAGRTRA
                     LFGLDGPYNLFVWRLDSI"
BASE COUNT          792 a         1413 c         1358 g          712 t
ORIGIN      
        1 tggcccggat gaccgcgccc gcttccaggc ggagttccag acccgaaccc agggtcagcc
       61 cgccgcagag atagcggccg gccggcaccc ggacccggcc gccgccggcc cggacgcagg
      121 cgtcgatcgc ccgctggaag gccgggtgct ccggcgccgg gccgccgccc cgggccccga
      181 aatcggtaac gcagaaatcc cgttctcccg ccatcgtctc tcctttcgcc gcccgttttt
      241 gtttggcagc tgccggccgg atatgttaca atttttgggt aaaacctgca atccgacccc
      301 caaacaacct gtaacccgga aggggctcca tgtccgaatt gtgcgtgatc atcctggccg
      361 ccggcaaggg aacccggatg aaatccgacc ttcagaaggt cctccatcaa ctgctgggca
      421 aacccatgat tcaccacctg gtcgaggagg tccgccggct ggagccggtc aaggtgctgg
      481 tggtggtcgg ccaccagaag gaagaggtga tgcagcggct ggaggggctg ccggtggagt
      541 tcgtggagca gaccgaaatg cggggcaccg gcgacgccgt gcgccgggcc gtcgaacgcc
      601 tgcccggata cgcgggcgag atcctggtcc tctgcggtga ctccccgctg ctgcgcagcg
      661 aaaccctgct gaaactgctt gaactccacc gccgggaagg gccggcggct accctgctga
      721 ccgcccgcct ggagaatccc accggttacg gccggatcaa gcgcaacggt accggtctgg
      781 tcgaggccat tgtcgagcag gccgacgcca ccgacgagga acggcggatc accgaagtga
      841 attccggcgt ttacgtcttc gcggcgcccc ggctctttga aaccctgccc gggctccggc
      901 ccgacaacgc caagggggag tattacctta ccgacgtcat aaagttcctg cgcacggccg
      961 gggaacgggt ggcggcccag gtggtggccg accccagcga aattctcggc gtcaacacac
     1021 ccgaagaact ggaaagggcc cggcaaatcc tggcccgccg gaggcaatca tgaacaactg
     1081 tcttctcttc accgggtcgg ccaaccagga attgggagcc gcgatcgccg cctacctgga
     1141 caagcaggtg ggccggatgg cgatcaccca cttcagcgac ggtgaaatca tggtggccat
     1201 tgaggagaac gtgcggggca aggatgtttt catcatccag tccacctgcc cgccggtcaa
     1261 tgaaaacctc atggaactgc tggtcatgat cgacgccttc aagcgggcca gcgccgagcg
     1321 gatcaccgcg gtcatcccct acttcgggta cgcccgccag gaccggaaag acaagccgcg
     1381 ggtcccgatc acggccaagc tggtcgccaa cctgctgacc gccagcggcg tcaaccggat
     1441 cctgaccatg gacctgcaca ctccccagat ccagggcttc ttcgacatcc cggtcgatca
     1501 cctcttcgcc gcgccggtaa cggtatccta ctttcaggag aagaaactgg accggatggt
     1561 ggtggtttcg cccgatgtcg gcagcgtcaa gatggcccgg gccttcgcga aggccctgcg
     1621 caccccgctg gcgatcgtgg acaagcggcg cgacggcccg acctcggtgg aggcggtcaa
     1681 cctgatcggc gaaatcgacg ggtcggtagc catcctggtc gacgacatga tttcgaccgg
     1741 tggcaccctg atggaggcgg ccgaggtgct gaagagcaag ggggctaccg ccatctacgc
     1801 cagctgctcc cacggggtgc tggtcggtga cgcggtggcc cggctggccg cctcctcgat
     1861 cgccgaactg gtgatcaccg acaccatccc cctcgcaccg gagaagaggc tggaaaagat
     1921 caaggtcctc tcggtggccg gcctcctggg cgaggccatc aagcgcatcc acctctccgc
     1981 ttcggtaagt tcgcttttcc tgtaaaaggg gggagagaac gccggcaagt caggccggcc
     2041 cccggcttac cgccgggggc cggtttttat ttcggggtca aatccaggaa cggtcgcgca
     2101 tcgccgatat caattccgag agggcaagca ggtaagcgcc gtcccgccag ctggccttct
     2161 gctccctgac ccgccgggcg acgtccttga aggcgccttc catcttggca tctatgaaat
     2221 cgtacgtctc ctgggccgaa gtctggttgc cgctcttggc gtttcgccat tccacgtagg
     2281 aggcgaccac gccgccgctg ttggccatga tatccgaaat gaccgggatt ttttttcggg
     2341 ccaggatctg gtcgccctcc ggggtggtcg gcccgttggc ggcttcgacc accagcttcg
     2401 cctgtacccc gggcgccgtc tcggcggtaa gcacatcctc gaccgcggcc ggaatgagca
     2461 ggtccaccgg cagggccagg atggcctggt tggtgatccg ttccaggccc tcagccggga
     2521 actcttccag cagcttcccc tggcgcacga aatccagaat ggcgggcacc ggcaggccgg
     2581 ccttggagta aaggccgccg gaaacatcgc tcaaggccac cacccgcgcc ccgctctcgt
     2641 gaaggaaggc ggcgctgtgg ctgccgacat tgccgcagcc ctggatggcg acactggcct
     2701 tggaaacatc gaggttgaga atttcccggc aggccaggcg cgaggcggta aagacgcctc
     2761 ggccggtcgc ttcgcgccgg cccggcagtc cgccgagccg gggcggcttg ccggtgacgc
     2821 tctcgggatg atgcgtctct ccgtagatga tcgccatgtc cgatggcgtc gaaccgaggt
     2881 cgggcgccgg tatgtaggcc ccggagacca gttcgtcccg gatcatgtgc acgaattcgc
     2941 ggatcagcat ccgtttttca aattcggaga tactgcgggg gttcaaggcg atcgaggact
     3001 tgccgccgcc gaagggaatt cccatcaggg cacacttgta ggtcatcagc tcggccaggg
     3061 tccgggtctc ggccagggtg acgttggggg caaaccggat gccgcccttg gccggcccca
     3121 gcaccaggtt gtggtaaacc aggtatgaat cgtagataag cagtttacct gcccccacct
     3181 tgatgttgac cgacatgatg acctgcttct ccggagcaga caacatcata cgggccgtct
     3241 ccgggatatc caggtacttt tgaagccttt cgaaatcgag catcacacac ctccttgaag
     3301 gtttcatccg gccggcggcc gctctctcct gaacgaggaa aaaggtcctg accgaggaat
     3361 aaccgcgcat tccggcgccc ggcggtcttc aaatgctgtc caggcgccag acaaaaaggt
     3421 tgtagggacc atccagaccg aaaagggccc gggtccgacc ggccgggtca agccgggcca
     3481 gagattccgg gtcgggctta aaatccggat tctgccggcc aaagatggcc agggtcttct
     3541 ccaggtcgag cacggccgcc atcaattccg agctcaggga ccagccggcg gccacgcttt
     3601 ggaatgcttc gatgccatcc gaaccggccg ccatcgaaaa ggtcagcgaa tcggttccaa
     3661 gccgctccat gactccggcc gcgacggcca ggacggtctc ggggcggccg ccgaattcga
     3721 ccggctgcca ggcgccccgg ccggtgacgg ccaggtaccc gaaatcctgg ccgcggccgc
     3781 cgccaaaaag aaacaggccc ggcttttcaa atatcagccg gtagtcgccc ggccgccgcc
     3841 gcaggcggaa cggatgtttt tcgtaagcgg ctcgaacctg gtcaagccag ccgggttcgc
     3901 ccaggtaacg ccggaccggg gctggcgtta ccccggccgc cttcaaactg cgggctgtca
     3961 accggaggct gagcgttcgg ccggcctgtt catagccaaa acggcggtaa cggaaacggt
     4021 cgccccagag gaccgagacc gggtagccgt cgccccgcat ccgctccagc gcccgttcca
     4081 tgagccgggt catgaaaccg cgcccccgca attccggccg ggtcgctaca ccgccgatgc
     4141 cggccgcctt cagccggacc ggaccctgcc ggagatcgag cggaaagatg cgcaccaggc
     4201 cgacgatgcg ttcggtgtcg tactggcccc agccttcgtt gaacggcacc cacatgatga
     4261 tggaggggtg gtttc
//
LOCUS       DAOQYN010000172         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_21782_length_4170_cov_4.278250, whole genome shotgun sequence.
ACCESSION   DAOQYN010000172 DAOQYN010000000
VERSION     DAOQYN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21782_length_4170_cov_4.278250"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..3300)
                     /locus_tag="PKN80_07010"
     CDS             complement(187..3300)
                     /locus_tag="PKN80_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35798.1"
                     /translation="MRGLRTLTIALGLVMALGCLSAVRAQESGTLGPWRVGDMAYNPT
                     LADDRPNAESAAIKARGFTPLIPDRVLKGTKGALSFRVSPRNEEWAGILVMDVEGKPL
                     GVRDGYSVQLVNPVDGKTAPFDVNDSRALFSDASRIFRPKTVIRVSQKNVLVDEATVD
                     FTSWKYPDSKVRMNDLLDGPTSAHGFVTARDGRFWREGKPIYFWGGHENHVPRNKAAS
                     DLYAEAYSAAGINIMRHIGVDEMVIDPVSGAVNPEMLDNYLYLVAKLGEKGIYFLMSG
                     YLGYQPSIYGLPKGSKGSNAFFWMDPAYRESWKKFLRILFTTKNPYNGKALKDDPTLI
                     GFELSNEMGMNERRFDYNRLDTLEETQLWRQAFNRFLLKKYGNREALARAWEINPLLP
                     NEDPTRGSILMPSNFRGVRSPYGGTGQHDQFTIGHWYRNGLPNEAAIRILDAIEFNQK
                     VAGKTYPFDFNNLETPEETGKLRAAFNQFLLQKYGDRAALAKAWAEDQLFGWEDPAKN
                     TILIPTNYRGEKEYRFDPSRRQADPRVSDALEFTYQVQKEWATDMARFLKDEIGLKCG
                     VGWNGDTFHVVQTPNHLANMNSPLDIAIAAAYLDWDNGDQITSRTKNLKRFTAYGRIL
                     GRPMHSYEWSMWTRQGPYTYEYGLLAALMGRVYGFDGYSHHKMAPVIYPISDPLYSLN
                     SHYITPLTDRRRGAFYVARWILERSRIPEAEKRLVIGFPAQSVFTGGPERRMSNWAFE
                     NWLMYQCGTEDYAFEDVYDGPADRVVVHSGHGPYGDYRKAQHAILWCHSNSDREGKDL
                     KAREKWFALHGIKFAPGQKYYLDDKYFATTEDLTDYNLVHARAEGARWDLLNKNAASK
                     QFGVVNTAAGDYWLPEKGQPLTEMDRKLYEALKRWGYPLPFEVEEIDQVWRSRDRTME
                     MNTLKTFFKSDRDDLQLWFGQLAENGGRVSLSRLEAVSGEKQYSAALIPWDTADFATA
                     RTLLLWTHWNSRVTVKLPFRGTPEIYAVNWLGKRLYRVKPLRSGRNELTFETCRDDDI
                     FGYEIAQ"
     gene            complement(3335..>4170)
                     /locus_tag="PKN80_07015"
     CDS             complement(3335..>4170)
                     /locus_tag="PKN80_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35799.1"
                     /translation="QDFYRGDLADRIAAAAEDKAAYVYTDYHKRHDYSIRALRHRYLA
                     GELGEALCASAYIEERREMPLKNFARWAQASNSFDYIGCHYVDVFYYVTGLRPKRVTA
                     YGQKKMLASLGHDIYDAVQALVEWENGAVLYVQTSWVLPDGNPNLTNQGFQMTGTQGE
                     YRADHADRGTNFCTQPSGYDRFNPYFCKPYRDWNHPERIVWEGYGIESVIQPLEDILS
                     LHQSAAGLSPERRLAKRREILAGLIKTRPLPEQAMVATAVLEAAHRSLAAGSVPVPVE
                     F"
BASE COUNT          733 a         1279 c         1285 g          873 t
ORIGIN      
        1 ctcctttcgc tgctcccccg aaattttacg ctcccccagt cattcttttg gccgaaaaac
       61 cttttcaacc cgtcttcttg aagatttgaa atttagttac cgggcgaagt tgaaacgttt
      121 atgccgccaa agaagagccc cccgatccgg ctcggagggc tcaaagacgc gcgagattta
      181 attcttttac tgggcgatct cgtagccgaa gatatcgtcg tcccggcagg tctcgaaggt
      241 caactcgttc cggccgctcc ggagcggctt gacccggtag aggcgcttgc ccagccagtt
      301 gaccgcgtaa atctcgggcg ttccccggaa gggaagctta acggtcaccc ggctgttcca
      361 gtgggtccag aggagcaggg tccgggcggt ggcaaaatcg gccgtgtccc agggaatcag
      421 ggccgccgaa tactgctttt cgccggagac cgcctcgagc cggcttaagg aaacccggcc
      481 gccgttctcg gccagctggc cgaaccaaag ctggagatca tcacggtcgg acttgaagaa
      541 ggtcttgagc gtgttcatct ccatcgtccg atcccggctg cgccagacct ggtcgatctc
      601 ctcaacctcg aagggcagcg ggtagcccca gcgcttcagg gcctcgtaga gtttccggtc
      661 catttcggtg agcggctgcc ccttctccgg cagccagtag tccccggccg cggtattcac
      721 caccccgaac tgcttcgagg ccgcgttctt gttgagcagg tcccagcggg ccccttcagc
      781 ccgggcgtgg accaggttgt aatcggtcag gtcctcggtg gtggcgaagt acttgtcgtc
      841 gaggtaatac ttctgaccgg gcgcgaactt gatgccgtgc aaggcaaacc acttctccct
      901 ggccttcagg tccttgcctt cccggtcgct gttgctgtgg caccagagga tggcgtgctg
      961 tgccttccgg tagtcgccgt aaggcccatg gccggagtgg accaccaccc ggtcggccgg
     1021 accgtcataa acatcttcaa aggcgtaatc ctcggttccg cactggtaca tcagccagtt
     1081 ctcaaaggcc cagttgctca tcctccgttc cgggccgccg gtgaagacgc tctgggccgg
     1141 aaagccgatg accagccgtt tctccgcctc cggaatgcgg gagcgctcca gaatccagcg
     1201 ggccacgtaa aaagccccgc ggcggcggtc ggtcaacggg gtgatgtagt gggagttcag
     1261 cgagtaaagc ggatcgctga tcgggtagat gaccggggcc atcttgtgat gggagtatcc
     1321 gtcgaagccg tagacccggc ccatcagggc cgccagcagt ccatattcat aagtgtaggg
     1381 cccctggcgc gtccacatgc tccactcgta ggagtgcatc ggccggccca gaatgcggcc
     1441 gtaggcggtg aagcgcttga ggttcttggt gcggctggtg atctggtcgc cgttatccca
     1501 gtccaggtag gcggccgcga tggcaatgtc caggggcgag ttcatgttgg ccaggtggtt
     1561 gggcgtctgg acgacatgaa aggtatcccc gttccagcct accccgcact tgaggccgat
     1621 ctcatccttc aggaacctgg ccatgtcggt cgcccattcc ttctgcacct ggtaggtgaa
     1681 ctccagcgcg tcgctgacgc gcggatcggc ctgacgccgg gaaggatcga accggtactc
     1741 cttttcgccc cggtagttgg tcgggatcaa aatggtgttc ttggccgggt cttcccagcc
     1801 gaagagctgg tcctcggccc aggccttggc gagagcggcc cgatcaccgt atttctgcag
     1861 cagaaactgg ttgaaggcgg cccggagctt cccggtctcc tcgggcgtct ccaggttgtt
     1921 gaaatcgaag gggtaggtct tgccggccac cttctggttg aactcgatcg cgtccaggat
     1981 ccggatggcc gcttcgttcg gcaggccgtt tcgataccag tggccgatgg taaactggtc
     2041 atgctggccg gtgccgccgt acggactgcg gacgccccgg aagttggagg gcataaggat
     2101 gctgccccgg gtcggatcct cgttcggcaa aagcgggttg atctcccagg cccgggccag
     2161 cgcctctctg tttccgtact tcttaagcag gaaacggttg aaggcctgcc gccagagctg
     2221 ggtttcttca agcgtgtcga gacggttata atcgaaacgc cgttcgttca ttcccatttc
     2281 attggaaagc tcgaatccaa tcagggtcgg atcatccttg agggccttgc cgttgtatgg
     2341 atttttggtc gtgaacagga tgcgaagaaa cttcttccag gactcccggt aggcgggatc
     2401 catccagaag aaggcgtttg accccttgct tcccttcggc aagccgtata tcgagggctg
     2461 gtatccgagg tagccgctca tcaggaagta gatgcccttt tcgcccagct tggcgaccag
     2521 gtagagataa ttatccagca tctccgggtt taccgcgccg ctcaccgggt cgatcaccat
     2581 ctcgtcaacg ccgatatggc gcatgatgtt gatgccggcg gccgaatatg cttccgcgta
     2641 caggtcggaa gccgccttgt tgcgaggaac atggttttcg tggccacccc agaagtaaat
     2701 gggtttccct tcccgccaga aacggccgtc ccgggccgtc acgaaaccgt gggcggaagt
     2761 cggtccgtcc agcaggtcgt tcatccggac tttcgagtcg ggatatttcc agctggtgaa
     2821 atcaaccgtc gcctcgtcta ccagcacgtt cttctggctg acccggatga ccgttttggg
     2881 ccggaagatc cggctggcgt cgctgaagag cgcccggctg tcgttaacgt cgaagggcgc
     2941 ggtcttgccg tcgaccgggt tgaccagctg gacgctgtat ccatccctca cccccagcgg
     3001 ctttccctcg acatccatga ccaggatgcc cgcccattcc tcgttccggg gcgatacccg
     3061 gaaactcagg gcgcctttcg tccctttcag cacccggtcc gggatgagcg gcgtgaaacc
     3121 acgggccttg atcgccgcgc tttcggcgtt gggccggtcg tcggccaggg tcgggttgta
     3181 ggccatgtcg cccacccgcc aggggccgag ggtcccactc tcctgggccc ggacggccga
     3241 taagcaaccc agcgccatta ccagtccaag cgctatcgtc agcgttctta atcccctcat
     3301 cgctcctcct ctggttgaaa gtaaaatccg cgggctaaaa ttcaaccgga accgggacgc
     3361 tcccggcggc caaactccgg tgggcggcct ccagaaccgc ggtcgccacc atggcctgtt
     3421 ccggcaaggg ccgggtttta atcagcccgg ccaggatttc acggcgtttg gccaggcgcc
     3481 gttcggggct gagaccggcg gccgactggt gcagggagag aatgtcctcc agcggctgga
     3541 tgacgctctc gataccgtag ccctcccaga cgatccgttc cgggtgattc caatcccggt
     3601 atggcttgca gaagtaagga ttgaaacggt cgtagccgga cggctgggtg cagaagttgg
     3661 tgccgcggtc ggcgtggtcg gcccgatatt caccctgggt cccggtcatc tggaagccct
     3721 ggttggtaag gttggggttg ccgtccggaa gcacccagga ggtctgaacg taaaggaccg
     3781 cgccgttctc ccattccacc agcgcctgga ccgcgtcgta gatgtcgtgg ccgagcgaag
     3841 ccagcatttt cttctgtcca taggcggtca cccgttttgg ccgcagcccg gtcacatagt
     3901 aaaaaacatc cacgtagtgg cagccgatat aatcgaagga attgctcgcc tgggcccagc
     3961 gggcgaagtt cttgagcggc atctcgcgtc gctcctctat gtaggcgctg gcgcagaggg
     4021 cctcgccgag ttccccggcc aggtatcggt ggcgcagggc ccggatcgag taatcgtgcc
     4081 gcttgtggta atcggtgtaa acgtaggcgg ccttatcctc ggccgcggcc gcgatccggt
     4141 ccgccaggtc tccccggtag aagtcctgcc
//
LOCUS       DAOQYN010000173         4122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_22112_length_4122_cov_3.945660, whole genome shotgun sequence.
ACCESSION   DAOQYN010000173 DAOQYN010000000
VERSION     DAOQYN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22112_length_4122_cov_3.945660"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PKN80_07020"
     CDS             complement(<1..547)
                     /locus_tag="PKN80_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNQ35800.1"
                     /translation="MRYLTLLFSVINIVSILSSLGIGFFVYSRNPHRRKHQLFLLLNL
                     VLAGWALAVFMVPLSGQNTIHILFWVRVSSAVAIFFPAVFYHFQVYTGAGPAGRIERR
                     KVISWYLLSSMLALASFSPLMVSKALLVPFNRTQLLPEVNYGRAFILMALYALGLMGY
                     SICELGRKRKTSQAIQRVEIEY"
     gene            complement(535..1752)
                     /locus_tag="PKN80_07025"
     CDS             complement(535..1752)
                     /locus_tag="PKN80_07025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNQ35801.1"
                     /translation="MKKRVAVTGCGVIAPNGIGRQKFWDALEKGLSGVRRITLFDPAG
                     FPCQIAGEVSDFQPLDYFDRKEIRKLARFSQFAAAAARMAINDARLTRAVDRRRPGLV
                     ALGVSTSALDFIEKEHRSFLKKGPASVSPYGILSASPHAATSEVIRLAGIPAVQVTLA
                     NECTSGLNAIGLAFQKIRDGEAALAICGGTDAPITPLMWAGFCQSGLVPFQNEQPATR
                     SRPFDLLRTVGIISEGAAIFILEELETARRRRAPVYAEIAGFGMSSEDSEAGVAAAMR
                     AALDQDRRRPEEVDYISAHAPSHTLIDRMEVRAVRQVFRQAARRLPVSSIKSMIGNPL
                     SAGGALQLASVLMAFDRSVIPPTINYEVPDPECDLDCVPNRARLNRINLALVNSHGIG
                     GSNSSLLLKKCVT"
     gene            complement(1749..3377)
                     /locus_tag="PKN80_07030"
     CDS             complement(1749..3377)
                     /locus_tag="PKN80_07030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ35802.1"
                     /translation="MHILLISMAGCPRTPADFIPDNGLALLAAGLARLGHTVEVADYN
                     TPEMVGRLFPENIRLELREIGRSLFDGRKPTPWQVWRLTVLERRLQRYQAAAYEELGG
                     ALAERVKREKFDWVGFKLWNGDGFTASLTMAACLKKELPRLPVFGGGPQVDIFRDRIF
                     EATGSFDALIYGEGEPTLQGLNDYAAGRRSLASVPNLIFKSGGSLTTTPREWVEDLNL
                     LPMADYRPEVYPALAGGRKLKLFVIDESRGCPYHCHFCIHPVKSGRPRRKSARAILAE
                     MESLRNSWGATLFRYAGSNTPADLLEEVADALTSRGLKIEYTAFSDLRKERIDFDRLR
                     ASGCYALFFGVESTDPTILDERLNKQASPEEMERLLKEARAAGIFTVVSLIVPNPGET
                     EATKQATLEFLERTRPDSVAVQFPGVIPNTEWARDPARYGFGIRRPEAYARRVMNYKI
                     RLFFPPRFWDPLPYRVDGKPFRVFARETEDFIRRVEARGFTTLVTDEYALMSRHAGLS
                     PVRFRDICRRAFFTGDADALQALVDRINENSRAL"
     gene            3586..>4122
                     /locus_tag="PKN80_07035"
     CDS             3586..>4122
                     /locus_tag="PKN80_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNQ35803.1"
                     /translation="MRQTIIAVIGLLMTASIARGAGFQNPVSGAAAAGQAGAFTAQAD
                     DASAVTHNPAGLTQLEKDQVLVTFSFISPSVKHITPLGVTEKAKGSTHQLPGFYWAAA
                     PSSANPVRFGLGISVPYGQSTRWSENSSFRYTGTFSEMELLDVNPAVAFQVNPRLSVG
                     LGLNYYSGRIKLEALDPYF"
BASE COUNT          777 a         1223 c         1342 g          780 t
ORIGIN      
        1 tgtattcgat ttcgacccgc tggatggcct gggatgtttt tcttttccgg ccgagctcgc
       61 agatggaata acccatcaag ccgagggcat acagggccat caggatgaag gcccggccat
      121 agttcacctc cgggagcagc tgggtccggt tgaaggggac cagcagggcc ttggagacca
      181 ttaacgggga gaaggaggcc agggccagca tagagctcaa caggtaccag ctgatcacct
      241 ttcgcctttc tatccggccg gccggcccgg cgccggtgta aacctgaaag tggtaaaaga
      301 cggccgggaa gaagatggcc actgccgagg agacccggac ccagaagagg atgtggatgg
      361 tgttctggcc ggagaggggc accatgaaga ccgccagggc ccagccggcc aggacaaggt
      421 tcaacaggag gaaaagctgg tgcttgcgcc ggtgcgggtt ccgggagtag acgaagaacc
      481 cgatacccag cgacgagagt atcgaaacga tgttgatgac cgaaaagagc agggtcaggt
      541 aacgcatttt ttcaggagca gcgaagagtt gctgccgccg atgccgtgag aattgaccag
      601 ggcgaggttg atccggttca ggcgcgcccg gttcggaacg cagtccaggt cgcattccgg
      661 gtcggggact tcgtagttga tggtcggcgg gatgacggaa cggtcgaagg ccatcaggac
      721 cgaggccagc tgcagggcgc ctccggccga gagcgggttg ccgatcatcg atttgatgga
      781 actgaccggc agccgtcggg ccgcctgccg gaaaacctgc cggaccgccc ggacctccat
      841 ccggtcgatc agggtatggg acggggcgtg ggccgagatg taatccacct cttccggccg
      901 cctccggtcc tggtcgaggg ccgcgcgcat ggccgccgcg acgccagcct ccgaatcctc
      961 gctgctcatg ccgaacccgg cgatttcggc ataaaccggc gccctccggc gccgggccgt
     1021 ctccagctcc tcaaggatga agatggccgc tccctcggat atgatcccga cggtgcgcag
     1081 caggtcgaac ggacggctcc gggtcgccgg ctgttcgttc tggaacggca ccagtccgct
     1141 ctggcagaaa ccggcccaca tcaagggggt gatcggggcg tccgtgccgc cgcagatggc
     1201 cagagccgcc tccccgtctc ggattttctg gaaggccagg ccgatggcgt tgagtcccga
     1261 ggtgcactca ttggcgaggg tgacctggac cgccgggatc ccggcgagac ggatgacttc
     1321 gctggtggcc gcgtgggggg aggcggaaag aattccgtac ggactgaccg aggccggacc
     1381 ttttttcaga aaggaccggt gctctttttc tatgaaatcg agggcgctgg tgctgacccc
     1441 gagcgccacc aatcccggcc gccgccggtc gaccgcccgg gtcagccggg catcgttgat
     1501 ggccatccgg gccgccgccg ccgcgaactg gctgaagcgg gccagcttcc ggatctcctt
     1561 gcggtcaaag tagtcgagcg gttgaaagtc ggagacctcc ccggcaatct ggcagggaaa
     1621 accggccggg tcgaagaggg tgatccgccg caccccggaa aggcccttct ccagggcatc
     1681 ccagaatttc tgccggccga tcccgttggg cgcgattact ccgcacccgg tgacggccac
     1741 tcgttttttc atagggcgcg gctgttttcg ttgatccggt cgacgagggc ctggagcgcg
     1801 tcggcgtcgc cggtgaaaaa ggcccggcgg cagatgtccc ggaagcgaac cggagacagg
     1861 ccggcgtgcc tggacatcag ggcatattcg tcggtgacca gggtggtgaa gccccgggct
     1921 tcaacccggc ggatgaaatc ctcggtttcc cgggcaaaga cccggaaggg tttgccgtcg
     1981 acccggtaag gcagcgggtc ccagaaccgg ggtggaaaga agaggcgtat cttgtagttc
     2041 atgacccgcc gggcgtatgc ttccgggcgc ctgattccga acccgtatct ggccggatcc
     2101 cgggcccact cggtgttcgg tatcaccccg gggaactgga ccgcgaccga gtccggccgg
     2161 gtcctttcca ggaattccag ggtggcctgc ttggtcgctt cggtttcgcc ggggttgggt
     2221 acgatcaggc tgaccacggt aaagatcccg gccgcccggg cctccttgag cagcctttcc
     2281 atctcttccg ggctggcctg cttgttcagg cgttcatcca gaatggtcgg atcggtcgat
     2341 tcgaccccga agaagagcgc gtagcacccg gaggcccgga gccggtcgaa gtcgatcctt
     2401 tccttgcgca ggtctgaaaa ggcggtatat tcgatcttga ggccgcggga ggtcagggca
     2461 tcggccactt cctccagcag gtcggccggc gtgtttgacc cggcatagcg gaacagggtg
     2521 gcgccccagc tgtttcgaag cgattccatt tcggccagga tggcgcgggc ggattttcgc
     2581 ctgggccggc cgctcttgac cggatggatg caaaaatggc agtggtatgg gcagccccgg
     2641 ctttcatcga tcacgaacag ctttagtttc cggccgccgg ccagggccgg ataaacctcg
     2701 gggcggtaat cggccatcgg cagaaggttg agatcttcaa cccattcccg gggcgtggtt
     2761 gtcaaagaac cgcctgattt gaatatcagg ttcggaaccg aagcgaggga gcggcggccg
     2821 gcggcataat cgttcaggcc ctggagggtc ggttcgccct ccccgtatat cagggcgtca
     2881 aagctgccgg tggcctcgaa gatccggtcc cggaagatgt ccacctgggg gccgccgccg
     2941 aatacgggca gccggggcag ttcttttttc agacaggccg ccatggtcag cgaggcggta
     3001 aaaccgtcgc cgttccagag tttgaagccg acccagtcaa acttttcccg cttcacccgt
     3061 tcggccagcg ccccccccaa ttcttcatag gccgccgcct ggtatcgttg aaggcgcctt
     3121 tccaggacgg tcagcctcca gacctgccag ggagtcggtt tccggccgtc aaagagggaa
     3181 cggccgattt ccctcaattc cagccggatg ttttccggaa agaggcgtcc gaccatttcc
     3241 ggggtgttgt aatcggccac ctccaccgtg tggccgagcc tggccagtcc ggcggccagg
     3301 agtgccagcc cattgtccgg gatgaagtcg gctggagtgc gcggacagcc ggccatgctt
     3361 atcaatagta tatgcatgaa cgattttgtg cgcctgccaa aaaaactgtt gtacaattct
     3421 gtggtgtcgt acaaaaatgt taaacccggt tggtgcagtt tgtcaagcaa gacattttct
     3481 taatccttga ttttaaaagg ggttaatgct ttaatcgccc cccggcggag caacgcttcg
     3541 tagtggcaaa aaaattgcat ctgatggggg tgaaaggagg cgtagatgcg ccagacaatt
     3601 atcgcggtaa tcggattgtt gatgacggct tcaatcgcca ggggagccgg tttccagaac
     3661 ccggtcagcg gagcggcggc cgcgggccag gccggagcct tcaccgccca ggccgacgat
     3721 gcttccgcag tcacccacaa cccggccggt ttgacccagc ttgagaagga ccaggtgctc
     3781 gtcaccttca gtttcatctc cccttcggtc aaacacatca cgccgctggg ggttaccgaa
     3841 aaggcgaagg ggagcaccca ccagctgccc ggtttttact gggcggcggc accctcctcc
     3901 gccaacccgg tccgtttcgg actgggaatc agcgttccct acggccagtc gacccgatgg
     3961 tccgaaaaca gttcgttcag gtacacgggc actttttcgg agatggaact gctggatgtt
     4021 aacccggcgg ttgcttttca ggttaacccg cgtctttcag tcggtctggg tcttaattac
     4081 tattcgggcc ggatcaagct ggaagccctc gatccctatt tc
//
LOCUS       DAOQYN010000174         4087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_22385_length_4087_cov_4.658482, whole genome shotgun sequence.
ACCESSION   DAOQYN010000174 DAOQYN010000000
VERSION     DAOQYN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22385_length_4087_cov_4.658482"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKN80_07040"
     CDS             <1..311
                     /locus_tag="PKN80_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNQ35804.1"
                     /translation="GLMAGNRYEPGRELRESFALMLINPAAFGEADFYSKEVNRFVEI
                     IKGGRRRPGVKEIRLPGERSFRSLARSEGLGIFLSGETLARLNDLAARNGLKPISLKS
                     "
     gene            complement(356..1129)
                     /locus_tag="PKN80_07045"
     CDS             complement(356..1129)
                     /locus_tag="PKN80_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35805.1"
                     /translation="MTGNKAAWGWVLSPAYIDYLSRRRWSLRQLYSPVSELAGFFHFR
                     NAVFFHPGPHEARLLAGLAEFNQVIGNVGDPSTLKDADQQAAKAREFSRLSLEFPNVI
                     GGIVDDFSTAVKGGRMPAELMRETFESLKSANTKLQLMAVVYTMHLDLDFSAYLPCLD
                     IVNLWVWKSSDLPNLDEYLKKAEERFPGKPIHLGLYLYDYGETCDTLPMSLVKFQLER
                     AREYLRTGRIKGFHLIGSYLKEELRSEPARWLAENLAGE"
     gene            complement(1203..1907)
                     /locus_tag="PKN80_07050"
     CDS             complement(1203..1907)
                     /locus_tag="PKN80_07050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ35806.1"
                     /translation="MTKQPGWLYDEAKTAGVDYADPARVEEYDRRHGKFRDYRKEAEA
                     IVKAIGIRPEDTVIDLGSGTGAFALNAAPYCRTLYAVDVSPVMLDYARRKAERAGLTN
                     VVFRPGGFLTYEHRGPAADALVSSAVLHHLPDFWKLIGLRRAARMLKTGGRFFLFDVV
                     FPIGLADCKKRLDDWVRAFAEKAGPEMAAEAEVHLRDEYSTYDRVLEEFLVQAGFRID
                     SAKYEDFLATYLCTRT"
     gene            2184..2966
                     /locus_tag="PKN80_07055"
     CDS             2184..2966
                     /locus_tag="PKN80_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNQ35807.1"
                     /translation="MSHCPEFDFHIHTRYLGCANATMEIPAIVNECRRVGVKSLAITD
                     HLNRLEFLPKHRPILNDIRALSEPGLEVYFGVELNFTGCDEGFAFNAEIKEEYGFQFA
                     IGGIHGTYLKEYDLAKLVEIQHRHHLKTCEDPLVEVLVHPYWFGTREFENNQWPWFAS
                     MKAVPDSYVRELGQTARETGTAIEINASTNLEFSRFPESYVKEYIEYLGRLAEEGVTF
                     SPASDSHDINSLQRISSAWKVIEALRIPPERVWRPGVKPLKK"
     gene            3097..>4087
                     /locus_tag="PKN80_07060"
     CDS             3097..>4087
                     /locus_tag="PKN80_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35808.1"
                     /translation="MKKETIKIGWAEADITPVKRIDLCGQYYPRLSRNIHSRLGLTVL
                     ALESESGEQAVLVSLDNVGISPGIMEMVRHRVKAAIPGIRPEKILVNATHVHTAPALY
                     PVVGWYEPDREVITPEEYREFLCSRLVGAVARAWSGRAPGAVAAGRGYAVTGHCRRAL
                     FEGGVAEMYGDAGRDDFLGLEGNEDSTLELLFTYDGRKRPTGVILNAACPSQVMEATY
                     SVSSDFMGEARRLLKKHFGAGFRTLCQVSAAGDQSPRDLVRNRTADFWGEKGVAVLGK
                     RLSDAVIEAQAGIGRDEITGRLEFCHQVASLPLPMRFPAPAELAAARKELKRLT"
BASE COUNT          764 a         1251 c         1272 g          800 t
ORIGIN      
        1 tcgggctgat ggccggcaac cgctatgagc cgggccggga gctgcgggag tccttcgcgc
       61 tgatgcttat caacccggcc gctttcggcg aggcggattt ttattcgaaa gaggtgaacc
      121 gctttgtgga gattatcaag ggcggccggc ggcggccggg cgtgaaggag atccgcctgc
      181 ccggggaaag aagtttccga tctcttgccc gaagtgaagg gctcgggatc ttcctttccg
      241 gcgagacgct tgccaggttg aacgacctgg ccgcccggaa cgggctgaaa ccgatctccc
      301 tgaaatcctg acgccttccc aaaaccgagt ttaccccccg gttttttctt ctccgctatt
      361 ccccggccag gttttcagcc agccagcgcg ccggttccga acgcagttcc tcctttaaat
      421 acgagccgat caggtgaaaa ccctttattc gtcccgtccg gagatactcg cgggcccttt
      481 ccagctggaa ttttaccagc gacatcggca gggtgtcgca ggtctccccg tagtcgtaaa
      541 gataaaggcc caggtggatc ggttttccgg ggaagcgctc ttcggccttt tttaggtact
      601 cgtcaaggtt cggcaggtcg gatgatttcc agacccagag gttgacgatg tccaggcagg
      661 gcaggtaggc ggaaaagtcc aggtcgaggt gcatggtata gacgacggcc atcagctgga
      721 gtttggtgtt ggccgatttc agggattcaa aggtttcccg catcagttct gccggcatcc
      781 ggccgccctt gacggccgtg ctgaaatcgt cgacgatacc tccgatcacg ttcgggaatt
      841 ccagcgaaag ccggctgaat tcccgagcct tggcggcctg ctggtcggcg tccttgagtg
      901 tcgagggatc cccgacgttg ccgatcacct ggttgaactc cgccagtccc gccaggagac
      961 gcgcttcgtg aggccccggg tggaagaaga cggcgttgcg gaaatggaag aagccggcca
     1021 gttccgagac cggtgaatag agctgccgca ggctccagcg ccggcggctt aagtaatcaa
     1081 tgtaggccgg gctcaacacc cagccccagg cggccttatt ccctgtcatc gttttctctc
     1141 ctgaaaaagc gcgggcattg cggccggcgc cgttttttta acctcgattc aagccgtccc
     1201 cctcaggtcc tggtgcagag gtaggtggcc aggaaatcct cgtacttcgc gctgtcgatc
     1261 cggaaccccg cctggaccag aaattcttcc aggacccggt cgtaggtgct gtactcatcc
     1321 cgaaggtgga cttccgcctc cgcggccatt tccggcccgg ccttttcggc gaaggcgcgt
     1381 acccagtcat ccaggcgttt tttgcagtcc gccaggccga tcgggaagac gacgtcgaag
     1441 aggaagaaac ggcccccggt tttcagcatc cgcgccgccc gccgcagccc gatcagtttc
     1501 cagaaatccg gcaggtgatg cagcacggcc gaagagacga gcgcgtccgc ggccggcccc
     1561 cggtgttcgt aggtaagaaa accgcccggg cggaagacca cgttggtcag gccggcccgt
     1621 tcggccttgc gccgggcgta atccagcatc accggcgaaa cgtcaaccgc gtaaagtgtc
     1681 cggcaataag gagccgcgtt cagggcgaaa gcgccggtcc cgctgcccag gtcgatgacg
     1741 gtgtcttccg gccggatccc gatggccttg actatggcct cggcctcttt ccggtaatca
     1801 cggaattttc cgtgccgtcg gtcatactcc tcaacccggg ccgggtcggc gtaatccacc
     1861 ccggccgttt tggcctcatc gtagagccat cccggttgct tcgtcatcag tttcgctccg
     1921 cgtcaaaagg gttgatttta caagatttag gatatagcgg ggcgggagga ttgtcaaggt
     1981 tccgaaggtt cgggagcgca ccggtctggt tgggttggcc cggtttttct gttgaatggc
     2041 ggatcgaatt aaggtaaact ttaatctgcg tccgggagac gcgcgacgcc ggccgacgac
     2101 cgggcgccat gaagaaggcc caggccggaa tcggccgggc ttcatttttg attatcacaa
     2161 tcggtttgaa tccggaggag acgatgagcc actgtcctga attcgatttc cacatacaca
     2221 ccaggtacct gggctgtgcc aacgcgacca tggaaatccc ggccatcgtt aacgaatgcc
     2281 gcagggtggg agtgaagtcc ctggccatca ccgaccacct caaccggctt gaattcctgc
     2341 cgaaacaccg gcccatcctg aacgatatcc gggccctctc cgaacccggc ctcgaggttt
     2401 atttcggcgt ggaactgaac tttaccggct gcgacgaggg gttcgccttc aatgcggaga
     2461 tcaaggagga atacgggttc cagtttgcca tcggcggcat ccacggcacc tacctgaagg
     2521 agtacgacct ggcgaaactg gtcgagattc agcaccgcca ccatctcaag acctgcgaag
     2581 acccgctggt cgaggtgctg gtccatcctt actggttcgg cacgcgggaa tttgaaaaca
     2641 accagtggcc gtggttcgcg tcgatgaagg ccgttcccga ctcttacgtg cgggaacttg
     2701 gccagacagc ccgggagact ggaacggcca tcgaaatcaa cgcctcgacc aacctcgagt
     2761 tttcgcgttt tcccgaaagc tacgtgaagg agtacatcga gtatctcggc cggctcgccg
     2821 aggaaggggt gaccttttcg ccggcctccg actcgcacga tatcaattcg cttcaaagga
     2881 tcagttcggc ctggaaggtc atcgaggcgc tgcgcatccc gcccgaacgg gtctggcgtc
     2941 ccggggttaa gcccttgaaa aaatgacgtt cggtccggcc gcccttttcc gaccggtcgg
     3001 ccaatccggg ttggattggg gagcgcttta aatctcgctt gttcgcaaac cgggaccggt
     3061 tgcggcacgc gcaaacattg acgggaaaac gcaaccatga agaaagaaac gattaaaatc
     3121 ggctgggccg aggcggacat cacgcccgtc aagcggatcg acctgtgcgg ccagtattat
     3181 ccccgccttt caagaaacat ccattcccgg ctcggcctga cggtcctggc cctggagtcg
     3241 gagtccgggg agcaggcggt gctggtttcg ctggataatg tcggcatctc ccccggaatc
     3301 atggagatgg tcaggcatcg ggtgaaggcc gccatcccgg gcatccgacc cgagaagatc
     3361 ctggtcaacg ccacccacgt tcacaccgcc cccgccctct acccggtcgt cggctggtac
     3421 gaaccggacc gggaggtgat caccccggag gagtaccggg aatttctctg ctcccgcctg
     3481 gtcggcgccg tcgcccgggc ctggtccggc cgggcgccgg gagcggtggc ggccggccgg
     3541 gggtacgcgg tcaccggcca ctgccgccgc gccctcttcg agggtggcgt cgccgagatg
     3601 tacggcgatg ccggccggga tgatttcctg ggcctggaag gcaacgagga ttccaccctg
     3661 gaactcctct tcacctacga tgggcggaag cggccgaccg gggtgatact caacgccgcc
     3721 tgtccttctc aggtcatgga ggcgacctac tccgtttcgt cggacttcat gggcgaggcc
     3781 cgccgtctgc tgaagaagca tttcggcgcc ggtttccgga cgctctgcca ggtctccgcg
     3841 gccggagacc agtccccgcg cgacctggtg aggaaccgga ccgcggattt ctggggggag
     3901 aaaggcgtgg ccgtcctggg caaacgcttg agtgacgcgg ttatcgaggc ccaggccggg
     3961 atcggaaggg atgagatcac cggccggctg gaattctgcc accaggtcgc ctcgctgccg
     4021 ctcccgatga gatttcccgc tccggccgaa ctggccgccg cccggaagga gctgaagcgg
     4081 ctgactg
//
LOCUS       DAOQYN010000175         4017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_22923_length_4017_cov_4.336699, whole genome shotgun sequence.
ACCESSION   DAOQYN010000175 DAOQYN010000000
VERSION     DAOQYN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22923_length_4017_cov_4.336699"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /gene="galT"
                     /locus_tag="PKN80_07065"
     CDS             <1..796
                     /gene="galT"
                     /locus_tag="PKN80_07065"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:NF027593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNQ35809.1"
                     /translation="NKFPALAIEGDLQRKGIGLFDQMNGIGAHEVIIETNRHVVDIAE
                     LSEAEILQVIGAYQSRSLDLKKDSRFRYILIFKNCGERAGASLEHTHSQIIATPVTPK
                     RVREELAGSQQHFLAKERCIFCDLLEQELDSGKRVIQETQHFMVLAPFASCFPFETWI
                     LPRRHHPDFDQISEAERQDLAAVLRKTIYRINRSLNNPPYNYLIHTAPNRYRRSGYWQ
                     TIDQDYHWHLEIIPRLTGIAGFEWGTGFYINPTAPEDAAAYLREID"
     gene            complement(825..2750)
                     /gene="glgB"
                     /locus_tag="PKN80_07070"
     CDS             complement(825..2750)
                     /gene="glgB"
                     /locus_tag="PKN80_07070"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNQ35810.1"
                     /translation="MKGRTAPETRPPMDKRLSDLELHLFSEGTLYRAYDSLGAQPVPA
                     GAGPGFRFAVWAPNARRVSVVGDFNGWDETRLRLEPAGTSGIWQGWTAEAAEGAFYKY
                     RIETAGGAVRLKSDPYAFAMELRPGTASIIHDTRRFQWTDGKWMADRFQRHQTGAPIS
                     IYELHLGSWDRRPDGWFLDYRELAPRLVEYLRPLGFTHVELLPVTEHALDESWGYQTT
                     GYFAPTSRHGQPEDFAWFVDYLHRHGIGVILDWAPAHFPADDHGLAWFDGTALYEHAD
                     PRQREHRDWGTHIFNYGRYEVANFLISSALFWLDRYHLDGLRVDAVASMLYLDYSRRP
                     GEWVPNRYGGRENLEAVAFLKRLNETIYRFFPDTCTVAEESTAWPKVSRPTWEGGLGF
                     GYKWNLGWMHDTLAYFRQDPLFRRYHQKKLTFGLIYAFSEHFILPLSHDEVVHGKGSL
                     LGKMPGDDWQKFASLRLLLGYLYTQPGRKLLFMGGEFGQWREWNCRSGLDWNLLQYEP
                     HRKLRDYLADLNRIYRNEPALHQLDSSPEGFRWIDFQDHRQNVIAFRRLGREPGEELL
                     VLGNFAPVLRRGYRVGVPEAGTYREILNSDSEYYGGSNAGNQGGRPSEAVPAHALPHS
                     LRLTLPPLALLVLKRDR"
     gene            3106..>4017
                     /locus_tag="PKN80_07075"
     CDS             3106..>4017
                     /locus_tag="PKN80_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35811.1"
                     /translation="MSDVLKVGVIGAGGIAQGGHIPSFQKQPGVEVAAVADPNEAKLG
                     EVVRKFQIGRSYRDYRDLLKEKDIKAVSICSPNFLHREQALAALKAGKHVLCEKPVAM
                     NTAETEEILATARETGLKFMVGFPNRFSATSVFLKKLVEAGQLGEIYFARTGWIRRRG
                     IPGLGGWFTTKSLSGGGPMIDIGVHMLDKAYWLMGAPRPVSVSGVTFQKFAEVATDGG
                     WPPAHTRLGDNFEGTFDVEDLAAAFVRFENGAVLSLEVSWAANAEANRYWHFFGTRAG
                     VREDFSGLKLFGEHSGIQTDTTPSLRDT"
BASE COUNT          696 a         1287 c         1335 g          699 t
ORIGIN      
        1 caacaagttc ccggcgctgg ccatcgaagg ggatctccag cgcaagggca taggtctctt
       61 cgaccagatg aacggcatcg gcgcccacga ggtgatcatc gagaccaacc gccacgtggt
      121 ggatatcgcc gaactctccg aggcggagat actccaggtc attggcgcct accagtcgcg
      181 ttccctggac ctcaagaagg acagccgttt ccgctatatc ctgatcttca agaactgcgg
      241 cgaacgggcc ggcgcctcgc tggagcatac ccattcccag atcatcgcca ccccggtcac
      301 ccccaagcgg gtacgcgagg aactggccgg cagccagcag catttcctgg ccaaggagcg
      361 ctgcatcttc tgcgacctgc tggagcagga gctcgattcc gggaaacgcg tcatccagga
      421 gacccagcac ttcatggtac tggcgccgtt cgcctcctgc ttcccgtttg aaacctggat
      481 actgccccgg cgccatcatc ccgacttcga ccagatctcc gaggcggagc ggcaggacct
      541 ggccgccgtc ctgcgcaaga ccatctatcg gatcaaccgg tcactcaaca acccgcccta
      601 caattacctc attcataccg cgcccaaccg ctaccggcgc tccggttact ggcagactat
      661 cgatcaggat taccactggc acctggagat tatcccgcgt ctgaccggca tcgccggttt
      721 tgaatggggc accggtttct atatcaaccc gaccgcgccc gaggacgccg ccgcctacct
      781 gcgcgagatc gattgaggga agacggaccg gcttgagttg aattttaccg gtcgcgcttg
      841 agcaccagca gcgccaacgg cggcagggtg agccggagcg agtggggaag ggcgtgggcc
      901 ggtaccgcct cgctcggccg gccgccctgg ttgccggcgt tgctgccgcc gtagtactcg
      961 gaatcgctgt tcagtatctc ccggtaagtt cctgcctccg gcacccccac ccggtaaccg
     1021 cgcctcagca ccggcgcgaa gttgccgagc accagcaact cttctcccgg ttcgcggccc
     1081 aggcgccgga aggcgatcac gttctggcga tggtcctgga agtcgatcca gcggaagccc
     1141 tccggagaac tgtccagctg gtggagggcc ggttcattgc gataaatccg gttgaggtcg
     1201 gccaggtagt cgcgcagctt gcggtgcggt tcgtactgga gcaggttcca gtccagtccg
     1261 ctccggcagt tccactcccg ccactggccg aactcgccgc ccatgaagag cagtttccgg
     1321 cccggctggg tgtagagata gccaagcagc agccggaggc tggcgaattt ctgccagtcg
     1381 tcgccgggca tctttcccag cagggatccc ttgccgtgga cgacctcgtc atgggagagc
     1441 ggcaggatga agtgttcgct gaaggcgtaa atgaggccga aggtcagttt tttctggtga
     1501 taacggcgga agagagggtc ctgccggaag taggcgaggg tgtcgtgcat ccagcccagg
     1561 ttccacttgt agccgaagcc gaggccgccc tcccaggtgg ggcgcgagac cttcggccag
     1621 gcggtggact cctcggccac cgtgcaggtg tccgggaaga agcggtagat ggtttcgttg
     1681 agccgcttca ggaaggcgac cgcctccagg ttttcgcgtc ccccgtagcg gttgggcacc
     1741 cattcgcccg gccggcgcga gtagtcgaga tagagcatcg aggcgaccgc gtcaacccgc
     1801 agcccgtcga gatggtagcg gtcgagccag aagagggcgc tgctgatcag gaaattggcc
     1861 acctcgtagc gcccgtagtt gaagatgtgg gttccccagt cccggtgttc gcgctggcgc
     1921 gggtcggcgt gttcgtagag ggcggtgccg tcgaaccagg ccaggccgtg gtcgtcggcc
     1981 gggaagtggg ccggcgccca gtcgaggatg actccgatcc cgtggcggtg caggtagtcg
     2041 acgaaccagg cgaagtcctc cggttggccg tgccggctgg tgggggcgaa gtagccggtg
     2101 gtctggtagc cccaggattc atcgagggcg tgctcggtga ccggcagcag ttcgacgtgg
     2161 gtgaagccga ggggacgcag gtactcgacc aggcgcgggg ccagttcccg gtagtccagg
     2221 aaccagccgt cgggccgccg gtcccaggag ccgaggtgca gttcgtaaat cgagatcggc
     2281 gctccggtct ggtggcgttg gaagcgatcg gccatccact tcccgtccgt ccactggaag
     2341 cgccgggtgt cgtggatgat cgaggcggtg cccggacgca gttccatggc gaaggcgtaa
     2401 gggtcgctct tgaggcggac cgccccgccg gcagtctcga tccggtactt gtaaaaggcg
     2461 ccttcggcgg cctccgccgt ccagccctgc cagatcccgc tggtaccggc cggttccagc
     2521 cggaggcgcg tctcgtccca gccgttgaag tcgccgacca cgcttacccg acgggcgttc
     2581 ggggcccaga ccgcgaaacg gaagcccggg ccggcgccgg ccgggaccgg ctgcgccccg
     2641 agcgagtcat aggcccggta gagggtgccc tcggagaaga ggtggagttc caggtcgctc
     2701 agtcgtttgt ccataggcgg ccgcgtttca ggggcggtcc ttcctttcat agattagcat
     2761 atcgtcaagc ggccgggtca aatgaccggc aacttttcag ccgcgccgtt tcccggccgg
     2821 ccgggccgcg gcaagcgcct cggccgggtg gaagagggcc gcgtgccatt cgggcttccg
     2881 ccgattttca tcaacaaccg cccccggcgc cgtcggccgg ggaacgagtg gcaaaacggc
     2941 ggattttcat cgctgggccg acccttgact tgcgcccggc caggacttat aatttctgaa
     3001 atcaacccgt ttccgccccg gcggaaaaag ggttgatcct cgcattcaag gcgggacccg
     3061 agcggtgtcg ccttctctgg ttatttcctc aaaggagaca aggccatgag cgatgttttg
     3121 aaggtcgggg tcatcggtgc cggcgggatt gcccagggtg gccacatacc gagttttcag
     3181 aagcagcccg gcgtcgaggt ggcggccgtc gccgacccga acgaggcaaa gctgggcgag
     3241 gtcgtccgca agttccagat cggccgttcc taccgggact accgcgatct gctcaaggag
     3301 aaggatatca aggcggtgag catctgctcg cccaacttcc tgcaccggga gcaggcgctg
     3361 gccgcgctga aggccggcaa gcacgtcctc tgcgagaagc cggtggccat gaacacggcc
     3421 gagaccgagg agatcctggc gaccgcccgg gagaccggcc tgaagttcat ggtcggcttc
     3481 ccgaaccgtt tttcggccac cagcgtcttc ctgaagaaac tggtcgaggc cggccagctg
     3541 ggcgagatat acttcgcccg gaccggctgg atccggcgcc ggggcattcc gggactgggc
     3601 ggctggttca ccaccaagtc cctttccggc ggcggtccga tgatcgacat cggcgtccac
     3661 atgctggaca aggcctactg gctgatgggc gccccccggc cggtttcggt gagcggggtg
     3721 acctttcaga aattcgccga ggtggcgacc gacggcggct ggccgccggc ccacacccgg
     3781 ctcggagaca atttcgaggg caccttcgac gtcgaggacc tggcggccgc cttcgtgcgg
     3841 ttcgagaacg gcgcggtcct gtcgctggag gtctcctggg cggccaacgc cgaggccaac
     3901 cggtactggc acttcttcgg aacccgggcc ggcgtccggg aggatttcag cggcctgaaa
     3961 ctcttcggcg aacattccgg catccagacc gacaccacgc cctccctgcg cgatacc
//
LOCUS       DAOQYN010000176         4016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_22929_length_4016_cov_4.754860, whole genome shotgun sequence.
ACCESSION   DAOQYN010000176 DAOQYN010000000
VERSION     DAOQYN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22929_length_4016_cov_4.754860"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2878
                     /locus_tag="PKN80_07080"
     CDS             <1..2878
                     /locus_tag="PKN80_07080"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35812.1"
                     /translation="PFEMPADARAAYSLVETDAGGFVGSSFFSLPALPEECCDDDPKN
                     DPPPPPPPPPPPGKPRTPPYTPPVVIPRDPNDIIGPAGFGEERWLPAASTLAYTIRFE
                     NAADASAPAQEVVITQQMDPDLDWRTFKVGDFGWGDLKVEIPAGQAKSFYSRRLDLRE
                     DYGFFVDVSAYLDIQTGEAKWTITTIDPETGEKPIDALVGFLPPKDGTGLGEGFVSYQ
                     VKPKRSVLTGDRIDAEARIVFDTENPIDTPPIFNTLDAAPPASQVEALPVRMAEPTFT
                     VAWNGGDDAGGSGLAGFTIMVSDNGGDYAAWLENTALTEAVFTGQPGHTYRFYSLARD
                     NAGNSEADPRQPLGTIEPDAATRVLNPFAVHAIAVAAGPNGSIDPAGPVVNVNEGQDA
                     AFNIIPNEGYHIESVTVDSQPVGAVSRYTFTGVVEPHSISAAFAINTYTLTVEYNTGG
                     RVEPAGPIIKEYGASQAFTITPDPGYRIDRVAADTEELGEVTGYTFENIRADHTLLVE
                     FAPVPVLSVTPTSIELLPSQAGAALTVTNTGLGTLNWSMAESLDWLSPDTDLGALAAG
                     QSQTVNLVRFGGSRGESGPITFTNASPKSGQAAIQVTVRIKDRPARPESVTLSYLPDL
                     TAVDTGSVGVDTPIAISGPAFSGADPADQLAAGQFEIRPETGDILVFSATVIEGELST
                     GSAFSRNILRPDLNYRLRLRYQDNLGAWSDWSDWFTLATVPAAATLDANSDGRPDSQE
                     PTPEELALAGLSDYAGLDTSRVLKVNGRMLLIRTEPPRTISYAGAFPAAEPPEYGLTP
                     YGVLIVRAEGVNGDTGVGIRYTFDSEPGTGSRWLGYRDTAAAGSRWSEIGAINIQGAD
                     LSAWVADAGGEDADRSANLVTLSLAALQTPGEPGDLNGDRRFDQTDLQLLLKMLIEKP
                     VTIRSLEYPSPYPAWLNDLADIDRDGAVTISDNLRLYRRIIGAD"
     gene            3052..>4016
                     /locus_tag="PKN80_07085"
     CDS             3052..>4016
                     /locus_tag="PKN80_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNQ35813.1"
                     /translation="MLYLKFNRLLLPALLAALLAAGPAAADDPAAGYAADEFLVRFRP
                     GISETEARAAVSSQRFEIVGRFRALSERRGQIFQHLKGARADASTLARLAADPRVERV
                     QLNYRRRPLQVPDDPRYPQQWALPAISAPAAWDITAGDSRVVIAIMDSGVDYLHPDLA
                     ANIWKNRAEWEGVDGVDDDGNGYVDDFQGYDFAATSRGANDNDPMDIDGHGTHLSGIA
                     AAIGNNGLGVAGVNWRASILPLKSMRPDGYFYDSDLIEAIEYAALMKRRFGVNIAAIN
                     ASLGDSAGYDGDFLSQAIELAGEAGIIFCAAAGNQGRNNDTRPVY"
BASE COUNT          700 a         1511 c         1239 g          566 t
ORIGIN      
        1 acccttcgag atgccggccg acgcccgggc cgcttacagc ctggtcgaga ccgacgccgg
       61 cggcttcgtc ggcagcagct tcttctcgct gccggccctc cccgaggaat gctgcgacga
      121 cgatcccaag aacgatccgc cgcccccgcc gccgcccccg ccgccgcccg gcaagccgag
      181 gacgccgccc tacacgccgc cggtggtcat cccgcgcgat cccaacgaca tcatcggacc
      241 ggccggcttc ggcgaggaac gctggctgcc cgctgcctcg acgctcgcct acaccatccg
      301 gttcgagaat gccgccgacg cctcggcccc ggcccaggag gtggtcatca cccagcagat
      361 ggatccggat ctcgactggc ggaccttcaa ggtgggcgat ttcggctggg gcgatttgaa
      421 ggtcgaaatc ccggccggcc aggccaagtc cttctacagc cgccgccttg acctgcgcga
      481 ggattatggc ttcttcgtcg atgtctccgc ctacctggac atccagaccg gcgaggcgaa
      541 gtggaccatc accaccatcg acccggagac cggcgaaaag ccgatcgacg ccctggtcgg
      601 cttcctgccg cccaaggacg ggaccggcct gggcgaaggg ttcgtctcct accaggtcaa
      661 gccgaaacgg agcgtcctga ccggcgaccg gatcgacgcc gaggcgcgca tcgtcttcga
      721 caccgagaac ccgatcgaca ccccgcccat cttcaacacg ctggacgcgg ccccgcccgc
      781 cagccaggtc gaggcgctgc cggtccggat ggccgagccg accttcaccg tggcctggaa
      841 cggcggcgac gacgcgggcg gctccggcct ggccggattc accatcatgg tctccgacaa
      901 cggcggcgac tatgccgcct ggctggaaaa caccgccctc accgaagcgg ttttcaccgg
      961 ccagcccggc cacacctacc gcttctacag cctggcccgg gacaatgccg gcaacagcga
     1021 ggccgacccg cgccagcccc tgggaaccat cgaacccgac gccgccaccc gggtcctgaa
     1081 ccccttcgcc gtccatgcca tcgcggtcgc ggccggcccc aacggctcga ttgatccggc
     1141 cgggccggtc gtcaacgtca acgagggaca ggacgcggcc ttcaacatca tcccgaacga
     1201 gggttaccac atcgagagcg tcacggttga cagccagccg gtcggcgccg tgagccggta
     1261 caccttcacc ggcgtggtgg aaccgcactc gataagcgcc gccttcgcca tcaacaccta
     1321 caccctgacc gtcgagtaca acaccggcgg ccgggtggag ccggccggcc cgataatcaa
     1381 ggagtatggc gccagccagg ccttcaccat cacccccgat cccggctacc ggatcgaccg
     1441 ggtggcggcc gacaccgagg aactgggcga ggtaaccggt tacaccttcg aaaacatccg
     1501 ggccgaccac accctgctgg tcgagttcgc cccggtcccg gtgctttcgg tcaccccgac
     1561 ctccatcgag ctgctcccga gccaggccgg cgccgccctg accgtcacca acaccggcct
     1621 gggcaccctg aactggtcga tggccgaaag ccttgactgg ctctcgcccg acaccgatct
     1681 cggcgccctg gccgccggcc agtcgcaaac ggtcaacctg gtccgtttcg gcggcagccg
     1741 cggcgagtcc ggcccgatca ccttcaccaa tgccagcccg aaatccggcc aggccgccat
     1801 ccaggtgacg gtgcggatca aggaccggcc ggcccggccc gaaagcgtga cgctgagcta
     1861 cctgccggac ctgaccgcgg tcgacaccgg cagcgtgggg gtcgacacgc cgatcgccat
     1921 ctccggcccg gccttcagcg gggccgaccc ggccgatcag ctggcggccg gccaattcga
     1981 aatccggccc gagacggggg acatcctcgt cttcagcgcc acggtcatcg aaggagaact
     2041 cagcaccggg tccgccttca gccggaacat cctgcggccc gatctgaatt accggctgcg
     2101 cctgcgctac caggacaacc tgggcgcctg gtccgactgg tcggactggt tcaccctggc
     2161 cacggtcccg gccgcggcca ccctggacgc caacagcgac ggccggcccg acagccagga
     2221 accgaccccg gaggagctgg ccctggccgg cctctccgac tacgccggtc tggacaccag
     2281 ccgggtgctc aaggtcaacg gacggatgct gctcatccgg accgaaccgc cccggacgat
     2341 cagttacgcc ggcgccttcc cggccgccga accgcccgag tacgggctga cgccctacgg
     2401 cgtgctcatc gtccgggcgg agggcgtcaa cggcgacacc ggcgtcggaa tccgttacac
     2461 ctttgattcc gaacccggca ccggcagccg ctggctcggc taccgggaca ccgcggccgc
     2521 cggcagccgc tggagcgaaa tcggcgccat caacatacag ggggccgacc tgtcggcctg
     2581 ggtggccgac gccggcggcg aggatgccga ccgcagcgcc aacctggtca ccctgagcct
     2641 ggccgccctc cagacgccgg gcgaaccggg cgacctcaat ggcgaccgcc gcttcgacca
     2701 gaccgacctg cagctgctgc tgaagatgct cattgaaaaa ccggtgacca tccgcagcct
     2761 ggagtacccg tccccttacc cggcctggct gaatgacctg gccgatatag accgggacgg
     2821 ggcggtcacc atctccgaca acctccgcct ctaccggcgg atcatcggcg ccgattgaac
     2881 ccggcccggg ccggcgaaga aaaaagggga ggccgaccgg cctccccttt tttttcagtg
     2941 aacatccgaa cccgccccga catctaatag aaccagacca ggcgtcttcc cggacgaccg
     3001 gccgcccggc tgtcaccgga agccgccccg ggtgtataat ctgaaggata tatgctatac
     3061 ctgaaattca accggcttct gcttccggct ctcctggccg ctctcctggc cgccggtccg
     3121 gccgccgccg acgatccggc cgccggttac gccgccgatg agttcctggt ccgtttccgt
     3181 ccgggcatct cggaaaccga agcccgggcg gcggtttcga gtcaacggtt tgaaatcgtc
     3241 ggacgcttca gggccctttc ggaaaggcgc ggccagattt tccagcacct gaagggcgct
     3301 cgggcggacg cctcgaccct ggcccgcctg gcggccgacc cgcgggtgga gcgcgtccag
     3361 cttaattacc ggcgccggcc gctccaggta cccgacgatc cccgttaccc gcagcaatgg
     3421 gccctgcccg ccatcagcgc cccggccgcc tgggacatca ccgccggcga ttcccgggtc
     3481 gtcatcgcca tcatggacag cggcgtcgat tacctgcacc ctgacctggc ggccaacatc
     3541 tggaagaatc gggccgagtg ggaaggcgtg gacggggtcg acgacgacgg gaacggctac
     3601 gtggacgact tccagggtta cgatttcgcg gccaccagcc ggggcgccaa tgacaacgac
     3661 ccgatggata tcgacggcca cggcacccac ctctccggca tcgccgccgc gatcggcaac
     3721 aacgggctgg gcgtggcggg agtaaactgg cgggcctcga tcctgccgtt gaagagcatg
     3781 cgccccgacg gctacttcta cgattccgac ctcatcgagg ccatcgagta cgcggccctg
     3841 atgaagcggc gcttcggcgt caacattgcg gccatcaacg cctcgctggg cgacagcgcc
     3901 gggtatgacg gcgacttcct ctcccaggcc atcgaactgg cgggcgaggc cggcatcatc
     3961 ttctgcgccg ccgccggcaa ccaggggcgg aacaacgata cccgaccggt ctacca
//
LOCUS       DAOQYN010000177         4000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_23039_length_4000_cov_5.060076, whole genome shotgun sequence.
ACCESSION   DAOQYN010000177 DAOQYN010000000
VERSION     DAOQYN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23039_length_4000_cov_5.060076"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="PKN80_07090"
     CDS             <1..1557
                     /locus_tag="PKN80_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35814.1"
                     /translation="NALSSGGGIGTLGLTNSRSLNFRITGGGTYRSVEKAEMIGQIDA
                     FYEALPLLVRLFISRNDFRGYMSQLLYQSTYEGGDNISLSALLESLTLPWNWAGLDFK
                     TTLDIKDATIPAVTRVTAKSGKNYRIQNIKNGQRLYLSGESAYTFNSVPESLRFLQYI
                     RTSLADAARSDTDLVTLTLSRPADVYVGLDPRMAAPAWLNGWENTGVTLGTTFTDAQP
                     MRLYRKNFPAGPVILGGVGSSDYAMYTIVVDAGRFDFGLEVISTARVGLMSDFDQAAV
                     IAPAARSEGGLTANSVSYLDDEILIEGTFVDEFDANVFLDSEANYDTGLDQLPINRLV
                     DQMLRNDGFRLIDTYNDQGPAFGVMDQRLLVESMFGLAINPPLEVELKQANLTTFTNM
                     NLLNSPNLVGLYLKSIATNDPTLALWLNTESPHSRKLNYSVIKPLFFDLPADRFTFFY
                     DSPENPVPYQEILRAIGRNQQGAEVTFGVRVRMDSGYNLDLRAAAFVTDYFTNIYDSV
                     VNQPFGGLIQ"
     gene            complement(1644..2810)
                     /locus_tag="PKN80_07095"
     CDS             complement(1644..2810)
                     /locus_tag="PKN80_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35815.1"
                     /translation="MKKMRWAFLSGIFLVLFFAGARVSAGAEAAAGNGVSLDFGGYYK
                     MLAGSIDVPLGGVYADYLNRLRLSLDAGFGPAVRARVEYDLELHLGDFSDSAYSRLFR
                     RVEAEPYWATGEVIHDDDDFFLEHSLYRGYLQVSLPAADLKIGRQGIDWGFGRAWNPT
                     NPFFPVNPLFIEKEEMAGTDAVTLLLPIGQTVSVELLAAGRPGLDQPALAGRGRGNFR
                     QTDYALTLYEDGELTLAGLDVSRTIAQAEVHGAVALADGPDSDYNSFTAGIDYGFPGT
                     TLKAGLEYFYNGAGERDEKRYDFLKLLSGDLQFAGRDYLFGGLDFEVNPNLRFQGCVV
                     SNLNDQGLFINPVLIYSAGEASSLSLGWAGFQAGKGDEFAFYPDIWYFQFQRFF"
     gene            complement(2807..3478)
                     /locus_tag="PKN80_07100"
     CDS             complement(2807..3478)
                     /locus_tag="PKN80_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ35816.1"
                     /translation="MIIAENISRVYDGVFPVRAVDGLSLEVADGEFLCLAGPSGSGKT
                     TLLHLLGGLERPDSGRIAIDGEEITGYNQRQRARLRLWKIGFIFQELNLIPVLNAREN
                     VEYGLLLQGVVASERRRRVDEILAELDLAGLAERRPRALSRGQQQRVAVARAMVSRPK
                     LVIADEPTANLDSHNAENLVELMRRLNRDRGVTIVMATHDQQIMKRVGRVVSLRDGQI
                     TGETK"
     gene            complement(3508..>4000)
                     /locus_tag="PKN80_07105"
     CDS             complement(3508..>4000)
                     /locus_tag="PKN80_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNQ35817.1"
                     /translation="EVLSWKEVAPDIVQLVELDIAMQTVLMIIISIIVALAIMNTMLM
                     AIQERFTEFGVMAAIGTRPGQIIAVLMTESFFLGLLGLAAGALVTMLGLIYLFWHGVN
                     LSAFSAGVMKLIGMDTTVRPLVQLKQVVVSSLVVLLSGTLVSILPALRAAHLEPVEAI
                     RHI"
BASE COUNT          798 a         1383 c         1144 g          675 t
ORIGIN      
        1 aacgccctct ccagcggcgg cggcatcggc acgctcggcc tgaccaactc ccgctccctg
       61 aatttccgga tcaccggcgg cggtacctac cgaagcgtcg aaaaggcgga aatgatcggg
      121 cagatcgacg ccttctacga agcccttccg ctcctggtcc gcctcttcat cagccggaac
      181 gacttccgcg gatacatgtc tcaactgctc taccagagca cctacgaggg aggcgataac
      241 atctcccttt ccgccctgct ggaatcgctc accctgccct ggaactgggc ggggctggac
      301 ttcaagacca cccttgacat aaaggatgcc accatcccgg ccgtcaccag ggtaaccgcc
      361 aagtccggca agaactaccg gatacaaaac atcaagaacg gccagcgcct ctacctttcg
      421 ggagagagcg cctacacctt caacagcgtt cccgaatcgt tgcgcttcct tcagtacatc
      481 cgcacctccc tggccgacgc ggcccgcagt gataccgacc tggtcaccct gaccctttcc
      541 cggccggccg atgtctatgt cggcctggat ccgcggatgg cggccccggc ctggctgaac
      601 ggctgggaaa ataccggggt aacccttggc accaccttca cggatgccca accgatgcgc
      661 ctctaccgga aaaatttccc ggccggcccg gtcatcctcg gcggcgtcgg ctccagcgac
      721 tacgccatgt acaccatcgt ggtcgatgcc ggccgcttcg atttcggcct cgaagttatc
      781 tcgaccgccc gggtcggcct gatgtccgat ttcgaccagg cagccgtaat cgcccccgcg
      841 gcccggagcg agggcggcct cacggccaac tcggtcagct acctcgacga tgaaatcctc
      901 atcgagggaa ccttcgtgga cgagttcgac gccaacgtct tcctcgacag cgaggccaat
      961 tacgacaccg gcctcgacca attgccgatc aaccggctgg tggaccagat gctccgcaac
     1021 gacggcttcc gcctcatcga tacctacaac gaccaggggc cggccttcgg ggtgatggac
     1081 cagcgcctgc tggtcgagtc catgttcggg ttagccatca acccgccgct ggaagtggag
     1141 ctgaaacagg ccaacctgac caccttcacc aacatgaacc tgctcaacag ccccaacctg
     1201 gtcggcctct acctgaagtc gatcgccacc aatgacccga ccctggccct ctggttgaat
     1261 acggaaagcc cgcacagccg gaaactcaac tacagcgtca tcaagccgct ctttttcgac
     1321 ctgccggccg atcgcttcac cttcttctac gacagcccgg agaacccggt gccttaccag
     1381 gagatcctgc gggcgatcgg acggaaccag cagggagccg aagtcacctt cggcgtgcgg
     1441 gtgcggatgg acagcgggta taacctcgac ctgcgggcgg cggccttcgt aaccgactac
     1501 ttcaccaaca tttacgactc ggtcgtcaac cagccgttcg gcgggttgat ccaatagaac
     1561 cccggccggc cccgggcaaa aaaacagcga ggcgccgctg acaagcggcg cctcgcctta
     1621 ttatccggcc cgaccgggca agtttaaaag aagcgctgaa actggaagta ccagatatcc
     1681 gggtaaaagg cgaactcatc acccttgccg gcctgaaaac ccgcccaccc cagggatagg
     1741 gaagacgcct cccccgccga atagatcaga accgggttga taaacaaacc ctggtcgttc
     1801 aggttgctca ccacgcagcc ctggaacctc aggttcgggt tcacctcgaa atccagcccc
     1861 ccgaaaaggt aatcgcgccc ggcgaactgc aggtcaccgc tgagcagctt cagaaagtcg
     1921 tagcgcttct cgtcacgctc cccggccccg ttgtaaaagt actccaggcc ggccttcagg
     1981 gtggtgcccg gaaacccgta gtcgatcccg gcggtaaagg aattgtaatc cgaatccggc
     2041 ccgtcggcca gagcgaccgc cccatgcacc tcggcctggg cgatggtccg ggagacgtcc
     2101 agcccggcca gggtcaattc gccgtcctcg taaagggtga gggcgtaatc ggtctgccgg
     2161 aagttgcccc ggccgcggcc ggccagcgcc ggctgatcca ggcccggccg cccggccgcc
     2221 aacagctcga cggagaccgt ctggccaatg gggagcagca gggtaaccgc atcggtaccg
     2281 gccatctcct ccttctcgat gaagagcggg ttgaccggga agaatgggtt ggtcgggttc
     2341 caggcgcgtc caaaacccca gtcgatcccc tgccgtccta tcttgaggtc ggccgccggc
     2401 aacgaaacct ggagatagcc gcggtagagc gagtgctcca ggaagaaatc gtcatcgtcg
     2461 tggatgacct ccccggtcgc ccagtagggt tcggcctcga cgcgccggaa gaggcgcgag
     2521 taggcgctgt cgctgaaatc tcccaggtgc agttccaggt cgtactcgac ccgggcgcgc
     2581 accgccggtc cgaacccggc atccaggctc aaccgcaacc ggttcaggta atcggcgtaa
     2641 acgccgccca gcgggacatc gatactcccc gccagcatct tgtagtaccc gccgaaatcc
     2701 aggctgacgc cgtttccggc cgccgcctcg gccccggccg aaacccgggc gccggcaaaa
     2761 aacagaacta aaaaaatccc ggacaggaat gcccatcgca tcttcttcat ttcgtctcac
     2821 ccgttatctg gccgtcccgg agggaaacga cccggccgac ccgcttcatg atctgctggt
     2881 cgtgggtcgc catgacgata gtcacgcccc ggtcccggtt aagtctgcgc atcagttcaa
     2941 ccaggttctc cgcgttgtgc gaatccaggt tggcggtcgg ctcgtcagcg atcaccagct
     3001 tcggccggga aaccatcgcc cgggccaccg ccacccgttg ctgctgcccc cggctcagag
     3061 cgcgcggccg ccgttcggcg aggccggcca ggtccagctc ggccaggatc tcgtccaccc
     3121 gccgccgccg ttccgaagcc accactccct ggagcagcag cccgtattcc acgttctccc
     3181 gggcgttcag caccgggatc aggttgagtt cctgaaagat gaacccgatc ttccagagcc
     3241 ggagccgggc ccgctgccgc tggttatacc cggtaatctc ctcgccgtca atcgcgatcc
     3301 ggccgctgtc cggccgttcc aaacctccca gcaggtgcag cagggtggtc ttgcccgaac
     3361 cggagggtcc ggccagacag agaaattctc cgtcggccac ctcgaggctg aggccgtcca
     3421 cggcccggac cgggaaaacg ccgtcgtaaa cccggctgat gttttcggcg atgatcattt
     3481 tcaattataa ccaaggggcg cccattttca aatgtgcctg atggcttcca ccggctccag
     3541 gtgggccgcc cggagcgccg gcaggatgga aaccagtgtg ccgctgagca ggaccaccag
     3601 gctggagacc accacctgct tcaattggac cagcggccgc accgtcgtgt ccatcccgat
     3661 cagcttcatc accccggccg aaaaggcgct caggttgacc ccgtgccaga aaaggtagat
     3721 gaggccgagc atggtgacca gggccccggc ggccagtccc agcaggccga gaaagaatga
     3781 ttcggtcatc aggacggcga tgatctggcc cggccgggtg ccgatggcgg ccatcacgcc
     3841 gaactcggta aagcgttcct ggatggccat cagcatggta ttcatgatgg ccagcgccac
     3901 gatgatcgag atgatgatca tcaggaccgt ctgcatggcg atgtccagtt cgaccagctg
     3961 gacgatgtcc ggggcgacct ccttccagct cagcacctcg
//
LOCUS       DAOQYN010000178         3927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_23669_length_3927_cov_3.870610, whole genome shotgun sequence.
ACCESSION   DAOQYN010000178 DAOQYN010000000
VERSION     DAOQYN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23669_length_3927_cov_3.870610"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKN80_07110"
     CDS             <1..214
                     /locus_tag="PKN80_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ35818.1"
                     /translation="EAGLPVLMDNRDRSAGEKFKDADLVGIPYQVVIGKRAVEGKIDF
                     RIRRTREQITMAPEEVVAKILAFPAE"
     gene            358..585
                     /locus_tag="PKN80_07115"
     CDS             358..585
                     /locus_tag="PKN80_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ35819.1"
                     /translation="MPTYEYECEKCRKRFEAFQKMSEPPLARCPDCAGEVKRLIGTGA
                     GIIYRGGGFYTTEYRSSDYQKRAKEEKENKA"
     gene            585..1661
                     /locus_tag="PKN80_07120"
     CDS             585..1661
                     /locus_tag="PKN80_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HNQ35820.1"
                     /translation="MYRVTLVPGDGIGPEITSVTKSVVDTLGVSIEWEEVPAGEEAMK
                     AYGTPMPEQLLASIDRNRVCLKGPITTPVGTGFRSINVALRQHFQLYANLRPVKLFPG
                     APSRYPEIDLVIVRENTEDLYAGIEFQHGQPETGELIDFVAAKKRGRIRADSGISLKP
                     ISITASERIARFAFEYAKKNGRRKVTAVHKANILKYSDGLYLECCRRVSREFPEIEFE
                     DRIVDNMCMQLVQKPELYDVLVLPNLYGDILSDLAAGLVGGLGITAGANIGEGVALFE
                     AVHGSAPKYRGLNKVNPTALIMAARLMLDYLGETKAGQRLENAVAKVIREGKSVTYDL
                     KPGRTGEAVGTIEMGRAIQAALEK"
     gene            1683..2960
                     /locus_tag="PKN80_07125"
     CDS             1683..2960
                     /locus_tag="PKN80_07125"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNQ35821.1"
                     /translation="MVTIVVGTQWGDEGKGKVIDFLAAGHQLIARYQGGSNAGHTVVV
                     NGKRYIFHLIPSGILHQGVECLLGNGVVIDPVSLETELQTLEKEGIPYAGRLWISENS
                     HVTLPSHKALDKMEDRFRGKGMLGTTGRGIGTTYADKSARIGIRMVDFIKPEVFRAKL
                     EINLQLKNYLFKKYYETDDIFQVDRIIEDYAPLAERLRPMVRDTAYYLNDALKSGRNL
                     LCEGAQGTFLDIDFGTYPYVTASNSIAGGACTGLGLGPTRINRVIGVAKAYTTRVGEG
                     PFPTEIAGVFGDRFRDLGAEYGATTGRPRRCGWFDSVLVRYSGIINGLDELFITKLDV
                     LSNLPSIKLATAYELNGARFDRPPLNFQDFSSIKPVYEEMEGWQTDLSGIRAWKQLPV
                     NARRYLDRIEELCQVPISTISFGPDRAQTLSRA"
     gene            3004..3774
                     /locus_tag="PKN80_07130"
     CDS             3004..3774
                     /locus_tag="PKN80_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ35822.1"
                     /translation="MNVLERFRLENRTALVTGGSRGLGLEIARALAEAGARIWLFSRN
                     PDTLAAVAARIENSTGRPVGFTAGDVTREADLEAALQSLLTACGRIDILVNSAGINIR
                     NPIEELEEADFRRVLEVNLVGTWLACRKVGPHLLRQRYGRVINLGSMFSTVAMPGRTA
                     YCASKGGVLQLTRTLALEWAPHGITVNCLCPGPFETEINEAIFRDPEIRRQFLENIPL
                     GRFGRPEELGPLAVLLAGDACPFMTGAALPVDGGWTSR"
     gene            3854..3926
                     /locus_tag="PKN80_07135"
     tRNA            3854..3926
                     /locus_tag="PKN80_07135"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3887..3889,aa:Lys,seq:ttt)
BASE COUNT          849 a         1243 c         1148 g          687 t
ORIGIN      
        1 cgaagccggt ctcccggtcc tgatggacaa ccgggaccgc agcgccggcg aaaagttcaa
       61 ggacgccgat ttggtaggga tcccctacca ggtggtcatc ggcaaacggg cggtcgaagg
      121 caaaattgat ttccggatcc gccggacccg cgagcagata acgatggcgc ctgaagaggt
      181 agtggcaaaa atccttgcct ttccggccga ataaacgaaa tgccggccgg tgaagaaaaa
      241 atcctttcct cgccaacttt ttgactttac ccattatttt taataaaata gaaggttggt
      301 ttgtattttt gccaatttca aattccagag caggttttcc tgcggggtga aagatccatg
      361 ccgacgtacg agtacgaatg cgaaaaatgc cggaaacgtt tcgaggcttt tcagaaaatg
      421 tccgagccac cgctggcgcg gtgtccggat tgcgcgggcg aagtcaagcg gctgatcggg
      481 accggcgcag gaataattta ccgcggcggc ggcttctaca ccactgagta ccgaagttcc
      541 gactaccaga aacgcgctaa ggaagaaaag gagaacaagg cctaatgtat cgagtaaccc
      601 tggtcccggg tgacggaatc ggcccggaga tcacttccgt gaccaagtcg gtggtggata
      661 cgcttggcgt ctcgatcgaa tgggaagagg tgccggccgg tgaggaggcc atgaaggcgt
      721 acggaacccc gatgccggaa cagctgctgg cctcgatcga tcgcaaccgg gtctgcctga
      781 aaggccccat aaccacgccg gtgggtaccg gtttccgttc catcaacgtc gcgctgcgcc
      841 agcacttcca gctttacgcc aacctgcggc cggtgaagct ctttccaggc gcacccagcc
      901 gctaccctga gattgacctg gtcatcgtgc gggaaaacac cgaagacctc tacgccggca
      961 tcgagttcca gcacggacag ccggaaaccg gcgaactgat cgatttcgtg gcggccaaaa
     1021 aacggggccg catccgggcc gattccggga taagcctgaa accgatctca ataaccgcct
     1081 ccgagcggat cgcccggttc gcctttgaat acgcgaagaa gaacggccgg cgcaaggtga
     1141 ctgccgttca caaggccaac atcctgaagt attccgacgg cctctacctg gagtgttgcc
     1201 ggcgggtaag ccgggaattt ccggagatcg aatttgaaga ccgcatcgtt gacaacatgt
     1261 gcatgcagct ggtacagaag cccgaacttt acgatgttct ggtactgccg aatctttacg
     1321 gagacatcct ttctgacctg gccgccggac tggtgggcgg actgggaata accgccggcg
     1381 ccaacatagg cgagggcgtc gccctcttcg aggcggttca cggaagcgcg ccgaaatacc
     1441 ggggcctgaa caaggtcaac ccgaccgccc tgatcatggc ggcgcgcctg atgctcgatt
     1501 acctgggcga gaccaaggcc gggcagcgcc tggaaaacgc ggtggccaaa gtcatccggg
     1561 agggcaagtc ggtcacttac gacctcaagc cgggccgaac cggcgaagcg gtcggcacca
     1621 ttgaaatggg ccgggccatc caggccgcgc tcgaaaaata agcgaaagag ataacattta
     1681 acatggtaac catcgtcgtc gggacccagt ggggtgatga aggcaagggc aaggtcatcg
     1741 atttcctggc cgccggccac caattgatcg cccgttacca gggcggcagc aacgccggcc
     1801 acaccgtggt cgtcaatgga aaacgatata tattccacct gatcccttcc ggcatattac
     1861 atcagggtgt cgaatgcctg ctgggcaacg gcgtggtgat cgacccggtc tccctggaga
     1921 ccgagctgca gacgctggaa aaggaaggta tcccttatgc cggccgcctc tggataagcg
     1981 aaaacagcca cgttaccctt cccagccaca aggccctgga caagatggaa gaccgtttcc
     2041 ggggcaaggg tatgctgggc accaccggcc ggggcatcgg caccacctac gccgacaagt
     2101 cggcccgcat cggcatccgg atggtcgatt tcatcaagcc ggaggtattc cgggccaagc
     2161 tggaaatcaa cctccagctc aagaattatc tcttcaaaaa atactacgag accgacgaca
     2221 tcttccaggt cgaccggatc atcgaagatt atgcgcccct ggcggaacgc ctgcgaccga
     2281 tggtccggga caccgcctac tacctgaatg acgcgctcaa atcgggccgc aacctcctct
     2341 gtgaaggggc ccagggaacc tttcttgaca ttgatttcgg cacctatccc tacgttaccg
     2401 ccagcaactc catcgccggc ggcgcctgca ccggacttgg cctgggaccg acccggatca
     2461 accgggtgat cggagtggcc aaggcctaca cgaccagggt gggggagggg cctttcccga
     2521 ccgaaatagc cggggtcttc ggcgaccgct tccgggacct gggcgccgaa tacggggcca
     2581 ccaccggccg gccgcgccgc tgcggctggt tcgacagcgt cctggtgcgc tactcaggaa
     2641 tcatcaacgg actggacgaa ctcttcatca ccaagctcga cgtcctcagc aacctgccat
     2701 ccatcaaact ggccaccgcc tatgaactga acggtgcgcg gttcgaccgg ccgccgctca
     2761 atttccagga tttttcaagc atcaagccgg tttacgagga gatggagggc tggcaaaccg
     2821 acctttccgg gatccgggcc tggaaacagc tgcccgtcaa tgcccggcgt tatctggacc
     2881 ggatcgagga actctgccag gtgccgatca gcaccatctc cttcgggccg gaccgggccc
     2941 aaaccctctc ccgcgcctga cacctccggc ccggccgtca ttgacaaaga ggcctttcct
     3001 gtcatgaacg tgctggaacg attccgcctc gaaaaccgga cggccctggt caccggcggc
     3061 agccgcggcc tgggcctgga aatcgcccgg gccctggccg aagccggcgc ccggatctgg
     3121 ctcttcagcc gcaacccgga cacccttgcg gccgtcgccg cccgaatcga aaactccacc
     3181 ggaagaccgg tcggcttcac cgccggcgac gtcacccggg aggcggacct ggaagcggcg
     3241 ctccagtcac tgcttaccgc ctgcggccgc atcgatatcc tggtcaacag cgccggcatc
     3301 aacatccgta acccgatcga ggaactcgaa gaggccgatt tccgccgggt cctggaagtc
     3361 aacctggttg gaacctggct cgcctgccgc aaggtcggtc cgcacctgtt acgccagcgc
     3421 tatggacggg taatcaacct gggctcgatg ttttcaacgg tggccatgcc gggacgcacc
     3481 gcctactgcg ccagcaaggg aggcgtcctt cagctcaccc ggaccctcgc cctggaatgg
     3541 gccccccacg gaatcaccgt aaactgcctc tgccccggtc cctttgaaac cgaaatcaac
     3601 gaagccatct tcagggaccc cgaaatacgc cgccagttcc ttgaaaacat cccgctgggc
     3661 cgcttcggcc gccccgagga actcggcccg ctggcggtac tcctggccgg agacgcctgc
     3721 ccattcatga ccggggccgc cctcccggtt gacggcggct ggaccagtcg ctgatcccca
     3781 gtccggttat tccaaccccg gcccggtatg gtaaaatata ccggcgctga cagacattca
     3841 gcccatgaat tccgggccgc tagctcatcc ggtagagcac cgccctttta aggcgggtgt
     3901 ggcaggttcg agtcctgcgc ggcctac
//
LOCUS       DAOQYN010000179         3910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_23793_length_3910_cov_4.730480, whole genome shotgun sequence.
ACCESSION   DAOQYN010000179 DAOQYN010000000
VERSION     DAOQYN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23793_length_3910_cov_4.730480"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2167)
                     /locus_tag="PKN80_07140"
     CDS             complement(<1..2167)
                     /locus_tag="PKN80_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35823.1"
                     /translation="MLKRTWLAGLLLLALSAAPAFGQQGRFSFTRLWTGEELQKLNLG
                     GSKFAGDPKEWKSAPGVGFSYNYPGTGPLPAPIVVKIPLGRSLPLGHYRLYVKNFYRG
                     KMEATLGDITKPLRIVRFDWTVAETFEINEPADQVTLRYFPSALVADTGARQSQGYIL
                     QGLFITTEANKVPKFASEIVELVTEDTPPARSGNYLENGGFEAGFTGWGKWYGSATPV
                     LRPTNLDGSGPAEGRYALRLEVPANDLRFGVETKLYRVPAGRDYTLSFAARADRPLAL
                     RAYLNGISEDLKNYSSAGLAAAVNLTNEWQRFTVSGRLLNRPGFLYTLVINPQTTDDR
                     PAVVWLDAVQLQEGAAAPFQPRTPREVGWLSATPGNVFYEREPARVELLVSGGGGNAA
                     VDYQTVDFWGQVVDRGRQEVAGGRAAFNLYAGKRGLFRTTFTLGEALSELVYSVLPAN
                     PHLNEKYPAGTLGVDMGFTPYHLDILKRANFNWVISKSLARWYRVEPEAQGKYLFFDE
                     AVRNADRANMMILLQTLSQGRETIPWLKDYVCPTGGAAWEPAKRAAYFKAWGDFIYAL
                     ASHYKPYVNHWEIDNEPNALYQQPEHYAEALRIASREIRRANPQAKVIGFSGGGFKRT
                     FYDPVVKNCPPENFDIVSVHFYGHANPDFATFLKEVGRPGWNTEVGSTGPTSFTSLPN
                     FDSVKEKEYWQLLQGTRQANAVSDVRTYLSSMSVGGIEKY"
     gene            complement(2191..3108)
                     /locus_tag="PKN80_07145"
     CDS             complement(2191..3108)
                     /locus_tag="PKN80_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ35824.1"
                     /translation="MIKTCKWTGRLGLVLLLMLAWAAQARADFRFVSLADSRGPSSDT
                     PVNVEVLERIARLVAAEKPALVVFGGDTINGGQKLNEKTIRNEYRIWKEALRPVYEAA
                     IPVYVIPGNHEMKGGRKHQALFREAFASNPDNGPAGSRKTAFSFDHDIARFICLDTAD
                     PDTPHRLLPEQTAWLDGQLASSRQTHIFVFGHDPAYPVGPHKGASLDKYPAERDAFWA
                     RLVKYRVPAYFCGHEHLYNRSRPEGVYQVINGTCGAPFRKKYAGEFYHYVVVDVSADR
                     ISFTVKDDQGKVHDRFEWTRPVPAPAAAK"
     gene            complement(3149..>3910)
                     /locus_tag="PKN80_07150"
     CDS             complement(3149..>3910)
                     /locus_tag="PKN80_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ35825.1"
                     /translation="QISAGEAKTRGDIYGTLGPIITAVNRLEPDLVLFVGDLVNIGGD
                     PATHKEQAAILYPVWTEEMKRLKAPYYTTPGNHDPHALYKQYIRPEVDYSVEKNGITF
                     IAFSSSAPENPDDWSHNGKVSPEQLAWLEKEMAKAAAKKNRIVTFTHITSHPNQHPLV
                     GWYIREGGPELRALYDRYGVFAEFSGHMHRWLANWKENKTWYIVAPGTMGGGYVAGAG
                     LAVYDILPDKAFQYEKPTRMPYFNSWGQWAKPELD"
BASE COUNT          600 a         1222 c         1338 g          750 t
ORIGIN      
        1 agtacttctc gatgccgccg accgacatcg agctcaggta ggtccgcacg tcgcttaccg
       61 cgttggcctg ccgggtgccc tgcaggagct gccagtactc cttctccttg accgagtcaa
      121 agttgggcag gctggtgaag gaggtggggc cggtcgagcc aacctcggtg ttccagccgg
      181 ggcggccgac ttccttcagg aaggtggcga agtccgggtt ggcgtggccg tagaagtgga
      241 ccgagacgat gtcaaagttc tccggtgggc aattcttgac caccgggtcg tagaaggtcc
      301 gtttgaagcc gcccccggag aagccgatta ccttcgcctg cgggttggcc cgccggatct
      361 cgcggctggc tattctcaag gcttcggcgt aatgctccgg ctgctggtag agggcgttcg
      421 gctcgttgtc gatctcccag tgattgacat agggcttgta atgggaggcc agggcgtaga
      481 tgaagtcgcc ccaggccttg aaatatgcgg cccgcttcgc cggctcccag gccgccccgc
      541 cggtcgggca gacgtaatcc ttgagccagg ggatggtctc ccgcccctgg ctgagggtct
      601 ggagcaggat catcatattg gcccggtcgg cgttccggac cgcctcgtcg aagaagagat
      661 acttcccctg ggcctccggt tcgacccggt accagcgggc cagcgatttc gagatgaccc
      721 agttgaagtt ggcccgcttc aggatatcca ggtggtaggg tgtgaaaccc atgtccacgc
      781 ccagggtgcc ggccgggtat ttttcgttca ggtgggggtt ggccggcagg accgagtaga
      841 ccagctccga gagggcctcg cccagggtga aggtggtccg gaagaggccg cgcttgcccg
      901 cgtaaaggtt gaaggcggcc cggccgccgg cgacctcctg gcggccgcgg tccaccacct
      961 ggccccagaa gtccaccgtc tggtaatcga ccgcggcatt accgccgccg ccggaaacca
     1021 gcagctccac ccgggccggc tcccgctcgt agaagacgtt gcccggcgtg gccgagagcc
     1081 agcccacctc ccggggcgtg cgcggctgga agggggccgc cgccccctcc tgcagctgga
     1141 ccgcgtccag ccagaccacc gccggtcggt cgtcggtggt ctgggggttg atgaccagtg
     1201 tgtagaggaa gccgggccgg ttgagcaagc ggccgctgac ggtgaagcgt tgccactcgt
     1261 tcgtcaggtt gaccgcggcc gcgagtccgg ccgatgaata gtttttgaga tcttccgaaa
     1321 tcccgttcag gtaggcccgc agggccagcg gtcggtcggc ccgggccgcg aagctcagcg
     1381 tgtagtcacg gccggccggt acccggtaga gcttggtctc caccccgaac cggaggtcgt
     1441 tggccggcac ctccagccgc agcgcgtagc gcccctcggc cggaccgctg ccgtccaggt
     1501 tggttggccg cagcaccggc gtggccgaac cgtaccactt accccagccg gtgaagccgg
     1561 cctcgaagcc gccgttctcc aggtagttgc cgctccgggc cggcggcgta tcctcggtta
     1621 ccagttccac gatctcactg gcgaacttgg gcaccttgtt ggcctcggtg gtgatgaaaa
     1681 ggccctgcag gatgtagccc tggctctgcc gggcgccggt gtcggccacc agcgcggagg
     1741 ggaagtagcg cagggtgacc tggtcggccg gctcgttgat ctcgaaggtt tcggccaccg
     1801 tccagtcgaa gcggacgatg cgcagcggct tggtgatatc gcccagggtc gcctccatct
     1861 tgccccggta gaagttcttg acgtagaggc ggtagtggcc cagcggcagg ctccggccga
     1921 gcggaatctt gaccacgatc ggcgccggca gcggcccggt gccgggatag ttgtaggaga
     1981 agccgacccc gggggccgat ttccattctt tcgggtcccc ggcgaacttg ctgccgccca
     2041 ggttaagctt ctgcagctcc tcgccggtcc agaggcgggt gaagctgaag cgcccctgct
     2101 ggccgaaggc cggcgcggcc gagagggcga ggagcaggag tccggccaac caggtccgct
     2161 taagcatgca atcctccttt tagtgttttt ttatttggcg gccgccgggg ccgggaccgg
     2221 ccgggtccac tcgaaccggt cgtggacctt gccctggtcg tccttgaccg tgaaggaaat
     2281 ccggtcggcg ctgacatcca ccaccacgta atggtagaac tcgccggcgt actttttccg
     2341 gaaaggcgcc ccgcaggtgc cgttgatcac ctggtagacc ccctcgggcc ggctgcggtt
     2401 gtaaagatgc tcgtggccgc agaagtaggc cggcacccgg tacttcacca gccgggccca
     2461 gaaggcgtcc cgctcggccg gatacttgtc gagggaagcc cccttgtgag ggccgaccgg
     2521 gtaggccgga tcgtggccga agacgaagat atgcgtctgg cgggacgagg ccagctgtcc
     2581 gtccagccag gcggtctgtt ccggaagcag ccggtgcggc gtatccgggt cggcggtgtc
     2641 caggcagatg aagcgggcga tgtcatggtc gaaggaaaag gcggtcttcc gggaaccggc
     2701 cgggccgtta tccgggttgg aggcgaaggc ctcccggaag agtgcctggt gtttgcggcc
     2761 gcccttcatc tcgtggttgc cgggtatcac gtagacgggg atggccgcct cgtagaccgg
     2821 tcgcaaggcc tccttccaga tccggtactc gttccggatt gtcttctcgt tcagtttctg
     2881 gccgccattg atggtatcgc cgccgaagac gaccagggcc ggcttctcgg ccgcgaccag
     2941 ccgggcaatc cgctccagca cctcgacatt gaccggcgtg tcggacgaag ggccccggct
     3001 gtcggccagc gagacgaatc ggaaatcggc ccgggcctgg gccgcccagg ccagcatcag
     3061 caggaggacg agcccgagcc ggccggtcca tttacaggtt ttgatcattt ttttctcgtt
     3121 tgttgttttc cgttgggctg tttttcattc agtccagttc cggttttgcc cactggcccc
     3181 agctgttgaa gtagggcatc cgggtcggtt tttcatactg aaacgcctta tccggcagga
     3241 tgtcgtaaac ggccaggccg gcgcccgcaa catagccgcc gcccatggtg ccgggggcga
     3301 cgatgtacca ggtcttgttc tccttccagt tggccagcca gcggtgcatg tggccgctga
     3361 actcggcgaa gaccccgtac cggtcataaa gggcacgcag ttccggcccg ccctccctga
     3421 tgtaccagcc gaccagcggg tgctggttgg gatggctggt gatgtgggtg aaggtgacga
     3481 tccggttctt cttggccgcc gctttggcca tctccttctc cagccaggcc agctgctccg
     3541 ggctcacctt gccgttgtgg ctccagtcgt ccgggttctc gggcgcgctg gagctgaagg
     3601 cgatgaaggt gatcccgttc ttctcgacgc tgtaatccac ctccggccgg atgtactgct
     3661 tgtagagggc gtgcggatcg tggttgccgg gagtggtgta gtaaggggcc ttgagccgct
     3721 tcatctcctc ggtccagacc gggtagagga tggcggcctg ctccttgtgg gtggccgggt
     3781 cgccgccgat attgaccagg tcgccgacga agaggaccag gtccggctcc agccggttga
     3841 cggccgtgat aatcggtccc agggtgccgt agatatcgcc cctggtcttg gcctccccgg
     3901 cgctgatctg
//
LOCUS       DAOQYN010000180         3890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_23972_length_3890_cov_3.370013, whole genome shotgun sequence.
ACCESSION   DAOQYN010000180 DAOQYN010000000
VERSION     DAOQYN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23972_length_3890_cov_3.370013"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PKN80_07155"
     CDS             <1..758
                     /locus_tag="PKN80_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNQ35826.1"
                     /translation="EAATFFIFFLGSINFGLHYLIWNGRRGELWKDLEIRTYVFTISI
                     TVLITLFGLIQMAGFQDLPSIFRTGFYQVLSGHTGTGFMTVTASQFAGWSELALLGVI
                     LAMGAGGCVSSTAGAIKALRIGLVAKGFLRQVKTVLTPEQGLVTEKFHHFKDVVLDNR
                     QVQTAGLLIIAYIGLYLAGTLVGMFCGYPFLPALFESVSAAANVGHTVGITGPAMPLA
                     LKLTYIIQMWVGRFEFLAIFVLAGFLYSWVRGR"
     gene            772..1269
                     /locus_tag="PKN80_07160"
     CDS             772..1269
                     /locus_tag="PKN80_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNQ35827.1"
                     /translation="MMTLLLAFLLAAGPALPDTPRVSVSDLVAHYQVYDGKPVLTQGE
                     AIGDLMLRGREGWVNISDGNAALGLYGTAETLKRIQYLGRFQSLGDRVVAAGIFHNAC
                     PVHSGQMDLHILRLEVVAEGKAVPEAFNFYKAGLAILLAIMAVGALLLRQFFRKATAG
                     RPKRS"
     gene            complement(1220..2512)
                     /gene="miaB"
                     /locus_tag="PKN80_07165"
     CDS             complement(1220..2512)
                     /gene="miaB"
                     /locus_tag="PKN80_07165"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNQ35828.1"
                     /translation="MTFFIRTFGCQMNQADSAGLAAVLAAAGHRPVSEPESAELIIFN
                     TCAVRDHAEERLFQNLAALRPRPGRPGPRVIGLVGCIPSLRGESLFNRFPHLSFLAGP
                     EQLDQVADLAARSAPGLRLAALGPGPAALPAVRGGGSVSAFLPVIRGCDNFCSYCVVP
                     YTRGREASRSASELLEAAAGLAAGGVRELILLGQNVNSYRDPAAGSGFPELLAAMAGL
                     DGLLRIGFLTSHPKDASEDLFRTMAEESRVYRHLHLPLQSGSDRILAAMNRRYTAAVF
                     SEKVVAARLRVADLSVTSDLIVGFPGETESDFQATLELLSAVRFDDLYAFKYSDRPGT
                     RAQELGEKVAETVKAERLARVWALQDAISRAANRERLGSEAEVLFLRRSGRKNGWLVG
                     KTGREQTVLVEGGPELIGRLGRVRIVSADRQLLYGRIA"
     gene            complement(2509..>3890)
                     /locus_tag="PKN80_07170"
     CDS             complement(2509..>3890)
                     /locus_tag="PKN80_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNQ35829.1"
                     /translation="LFLLVGILAGSEGLGGIYFDDYGLAKTIGIVALIFIIYYGGLDT
                     AWKSVKPLLFPGLALSTFGVLVTAFTVGLFAMAILKFSPLEGLLLGSIVSSTDAAAVF
                     SVLRSRRISLKGDLKALLELESGSNDPMAVFLTIALISLLTGENAPGANLLLQFALNM
                     GLGLLAGYLMARLGVFVINRIKLEYEGLYQVLIAALILLTYSVANFSRGNGFLAVYVL
                     GLMMSRSAFIHKRQSVRFYEGVAWLMQIAMFLTLGLLVFPSQIKPIIGPGLLISAMLI
                     FLGRPISVFLCLLPFRFQFKEKVLISWVGLRGAVPVILATFPLLAGVAQAHTIFNIVF
                     FVVLTSVLVQGTSIPVVSKRLGLDIPFDRKKRYPLELDETEGIEASLNDLIVPYNSAA
                     VGQPIASIDLPPQALIVLISRAEHFIIPTGSTVIEAGDVLLVLGKDSDFSQVQATLSK
                     LREPEEKSS"
BASE COUNT          787 a         1236 c         1204 g          663 t
ORIGIN      
        1 acgaagcggc caccttcttc atcttcttcc tgggttcgat caatttcgga ctgcactatc
       61 tgatctggaa cggccggcgg ggcgaactct ggaaggacct ggagatcaga acctacgtct
      121 tcaccatttc catcacggtg ctgatcaccc tcttcggact gatccagatg gccggcttcc
      181 aggacctgcc gtccattttc cggaccggct tctaccaggt gctatccggc cacaccggaa
      241 ccggtttcat gaccgtcacc gccagccagt tcgccggctg gagcgaactg gccctgctgg
      301 gtgtcatcct ggccatgggc gccggcggct gcgtctcctc caccgccggc gccatcaagg
      361 ccctgcgcat cgggctggtg gccaagggtt ttctgcgcca ggtcaagacg gtcctgaccc
      421 cggagcaggg cctggtaacc gaaaaattcc accacttcaa ggatgtggtg ctggacaacc
      481 gccaggtcca gacggccggc ctgttgatca ttgcttacat cggcctttac cttgccggca
      541 ctctggtcgg gatgttctgc ggatacccct tcctgccggc gctcttcgaa tcggtctcgg
      601 cggccgccaa cgtcggccat actgtcggca ttaccggtcc ggccatgccg ctggcactca
      661 agttgaccta catcatccag atgtgggtgg gccggttcga gttcctggcc atcttcgtcc
      721 tggccggatt tctttactcc tgggtgcggg gacgataatg gggttgcgaa gatgatgacc
      781 ttgctgctgg cttttctcct ggccgctggg ccggccttac ccgacacacc gagggtcagc
      841 gtctcggacc tggtcgccca ttaccaggtt tacgacggga aaccggtgct tactcagggc
      901 gaggcgatcg gcgacctgat gctcaggggc agggagggct gggttaacat cagcgatggc
      961 aacgccgcgc tgggacttta tgggaccgcg gaaacgctga aaaggattca gtacctcggc
     1021 cgcttccagt cgctgggcga ccgggtcgtg gccgccggta tctttcacaa cgcctgtccg
     1081 gttcattccg gtcagatgga ccttcacatc ctgcggctgg aggtggtcgc tgaaggaaaa
     1141 gccgttccgg aggccttcaa cttctacaag gcaggcctgg cgatactgct ggcgatcatg
     1201 gccgtgggcg ctctgctgct caggcaattc ttccgtaaag caactgccgg tcggccgaaa
     1261 cgatcctgac ccggcccagc cgaccgatca attccggacc gccctcaacc agcaccgtct
     1321 gttcccggcc ggtctttcca accagccaac cattcttgcg gccgctccgg cgcaggaaca
     1381 acacttccgc ctcgcttccc aaccgttccc ggttggccgc ccgggaaatc gcatcctgaa
     1441 gggcccagac ccgggccaga cgttccgcct tcaccgtttc ggccaccttc tcccccagtt
     1501 cctgcgcccg cgtgccgggc cggtcggaat atttgaaggc gtaaaggtcg tcaaagcgaa
     1561 ccgccgaaag aagttcgagc gtggcctgga aatcagactc ggtctcgccg gggaagccga
     1621 cgatcaggtc cgaggtgacc gataaatccg ccacccggag ccgggccgcc accacctttt
     1681 ccgaaaaaac ggccgcggtg tagcggcggt tcatggccgc cagaatccgg tcggaaccgg
     1741 actggagcgg caggtgcagg tggcgataga cccgggattc ttcggccatc gtccggaaaa
     1801 ggtcctccga ggcgtccttg gggtgggagg tcagaaaacc gatcctgagc aggccgtcca
     1861 gcccggccat ggccgccagg agttcgggga acccggaccc ggccgctggg tcgcggtagg
     1921 agttcacgtt ctgtcccagc agaatcagtt cccggacgcc gccggccgcc aggccggcgg
     1981 ccgcctccag caactcggag gccgaccggc tcgcctcacg cccgcgcgtg taggggacca
     2041 cgcaataact gcagaagttg tcgcagcccc ggatcaccgg aaggaaggca ctgacggaac
     2101 cgccgccgcg gacggccggg agggcggccg gtccgggccc cagggcggcc agccgaagcc
     2161 ccggagcgct ccgggccgcc agatccgcca cctgatccag ctgttcggga ccggccagaa
     2221 aggagaggtg gggaaagcgg ttgaagaggc tctctccgcg cagggaagga atacagccga
     2281 ccaggccgat cacccgcggt cccggccgac ccggccgggg ccgcagggcg gccaggtttt
     2341 gaaagaggcg ctcctcggcg tgatcgcgaa ccgcgcaggt gttgaaaata atcagttcgg
     2401 ctgattccgg ctccgaaacc ggccggtggc cggccgccgc cagcacggcg gccaggccgg
     2461 cactgtcggc ctgattcatc tgacagccga aggtcctgat aaagaaggtc acgaactctt
     2521 ctcctccggc tccctcaatt tcgagagcgt cgcctggacc tggctgaaat ccgaatcctt
     2581 gccgagcacc agcagaacat ccccggcctc gatcacggtt gaaccggtcg gaatgataaa
     2641 atgctccgcg cgggaaatga gcacgatcag cgcctgcggg ggcaggtcga tcgaggcgat
     2701 cggctggccg accgccgccg aattgtacgg tacgatcagg tcgttaagcg aggcctcgat
     2761 gccctcggtc tcatccagct caagcggata cctcttcttc cggtcaaacg gaatatcgag
     2821 accgagtcgc ttcgagacga ccgggatcga agtcccctgg accagcaccg aggtcagaac
     2881 cacaaaaaaa acgatgttga agatggtgtg ggcctgggcc actccggcca ggagcggaaa
     2941 ggtcgccagg atgaccggca ccgccccccg aaggccgacc caggagatca gcaccttctc
     3001 cttgaactga aaccggaaag gaagcaggca gaggaagacg ctgattggac gccccaggaa
     3061 aataagcatg gcggagatca gcaggccggg gccgatgatc ggtttaatct gggaagggaa
     3121 gaccagcagc ccgagcgtca ggaacatcgc aatctgcatc aaccaggcga ctccctcgta
     3181 aaaccgcacg gactgccgct tgtggataaa agcgctccgg ctcatcatca agcccagcac
     3241 gtacactgcc aggaaaccat ttccacggct gaaattggcg accgaatagg taagcagaat
     3301 cagggcggca atcaaaacct ggtagagccc ttcgtattca agcttgatcc ggttgatcac
     3361 gaaaacgccc agccgggcca tcaggtaacc ggccagcagt cccagcccca tgttcagcgc
     3421 gaactggagc agcaggttcg ccccgggggc attctcgccg gtcaaaaggc tgatgagggc
     3481 tatcgtcagg aagacagcca tcggatcgtt gctgcccgac tcaagttcca gcagggcctt
     3541 cagatcaccc ttgagtgaaa tccgcctgga gcgcagcacg ctgaagaccg cggccgcgtc
     3601 ggttgaggag acgatggaac cgagcagcag accttccagg ggggagaatt tcagtatcgc
     3661 catggcgaag agtccgaccg tgaacgcggt gaccaggact ccgaaggtcg aaagggccag
     3721 gccggggaag agcagcggtt ttaccgattt ccaggcggta tccaggccgc cgtagtagat
     3781 gatgaaaatc agcgcgacga tgccgatcgt tttcgccagg ccgtaatcat cgaagtaaat
     3841 gccgccgagc ccctccgagc cggccaggat cccgaccagg aggaagagca
//
LOCUS       DAOQYN010000181         3862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_24247_length_3862_cov_4.548989, whole genome shotgun sequence.
ACCESSION   DAOQYN010000181 DAOQYN010000000
VERSION     DAOQYN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24247_length_3862_cov_4.548989"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PKN80_07175"
     CDS             complement(<1..818)
                     /locus_tag="PKN80_07175"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ35830.1"
                     /translation="MYRIGREEIEELARVIESRKLFRTGDPKQGHLQEVERFEQEWAR
                     TMGSRYALCLSGGGTAALLCGLAGLGIGPGDEVIVPGYTFMATASAVLAAGAIPVLAE
                     VDETLGLDPEDLERKIGPATRAVIPVHMVGLPCNLRRIREIADRRGLKVIEDSCQADG
                     GSFGGRRLGTWGEVGTYSFNYYKVISCGEGGAVVTDDRAVYERMAIFADSGMAFRKYA
                     SELSVPIFIGLQLRASELMGAVMRIQLQRLDGILTDLRHRKRLFIESLAGAPGI"
     gene            complement(855..1757)
                     /locus_tag="PKN80_07180"
     CDS             complement(855..1757)
                     /locus_tag="PKN80_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNQ35831.1"
                     /translation="MGKTSGLVFDIQRFSLHDGPGIRTTVFLKGCPLRCRWCHNPESW
                     SPKPVLLFTSRLCVGCGACRAACPRGAHSVEGKRRFDRSLCAACGRCVGGCPTGALRL
                     AGRRLEVKEVVSDVLRDRLFYEYSGGGMTVSGGEPLFQAGFTLALLKAARREGLHTAV
                     ETCGFGPFDRLEALLPVTGLFLFDIKAGASRHRRQTGVPAAGILENLERLARAGARIW
                     LRVPLVPGFNVGPELFKLVAGLARELPGVERVELLPYHRLGEGKYLDLGLANPAAGLT
                     APSKARVSSWLGKFERLGIQAGVV"
     gene            complement(1769..>3862)
                     /locus_tag="PKN80_07185"
     CDS             complement(1769..>3862)
                     /locus_tag="PKN80_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNQ35832.1"
                     /translation="DDFKAMTERKNWEKLRGRARRRRRGEFDLRPYWRVLAYRASAGE
                     PEPIFRARAFASVLANLPAAFEPGERLAGDPRFFFAEKLPEGVGRAEYQAVLAEHQER
                     GQRDFRAGFDHTLAHYPDLLRLGIAGLAARARRERDRRRPGPARDFLESAVITLEAFS
                     GLARRFAREAGRSGRRELAAGLNRIAGAPPRSFHEALQLVWLTHLAFASEGRKHMALG
                     RLDQYLYPFYRRDLRAGRITRPAALDLLCHLWVKLDGFGQVQNICIGGLTPAGRDAVN
                     ELSYLCLEATRLVQTPYANLSARFHDATPERFHLACLEVIKTGIGFPAIFNDEVLVPA
                     LVRIGIPLPAARDHCFVGCIETMLPGRQPAWSDSRFNLLACFSEALELFGPPPGSSYR
                     KLWRLFESVLRKRLAGHVRSVNEYIERFPAGKFPDPFLSALTRDCLRRGRDVNDGGAV
                     FPRFHGIAGMGLASVADSLAAVRRLVFESGEVSLPALRKALAADFQGGEALRLKLLNR
                     APKYGNDDSYVDDIAAAVVRLFTSACLEHRLAGGGRFLGLMAANVSNIPAGREVGATP
                     DGRRAGAPLSDAASPYFGRDRRGPTAFIGSVARPDYTGAVGGSVINMKFEPAFFRGPE
                     GTRLLSAFTRSFVARRIQELQFNFTGNELLEAARRDPEKHRNLIVRVSGFSEFFVNLS
                     PEVQADVIRRRAHGG"
BASE COUNT          703 a         1312 c         1298 g          549 t
ORIGIN      
        1 ctgatgccgg gggccccggc cagcgactcg atgaagaggc gtttccgatg gcgcaggtcg
       61 gtcaggatcc cgtccagccg ctgcagctgg atgcgcatca ccgcacccat caactcgctg
      121 gccctcaact ggaggccgat gaaaatcggc acagagagtt cggaggcgta cttgcggaag
      181 gccatcccgc tgtcggcgaa gatggccata cgttcgtaaa ccgcgcggtc atcggtcacc
      241 accgccccgc cctctccgca gctgataacc ttgtagtaat tgaagctgta agtgccgacc
      301 tctccccagg taccgagccg ccgccccccg aagctgccgc cgtcggcctg gcagctgtcc
      361 tcgataacct tgaggccgcg ccgatcggcg atctcccgga tgcgccgcag gttgcagggc
      421 aggccgacca tgtgcaccgg gatgaccgcc cgcgtcgccg ggccgatctt ccgttccagg
      481 tcctccgggt ccaagccgag cgtctcgtca acctcggcca gcaccgggat ggccccggcg
      541 gccaggaccg ccgaggcggt ggccatgaag gtgtagccgg gcacgatcac ctcgtcgccc
      601 gggccgatcc ccaggccggc caggccgcac aggagggcgg ccgtgccgcc cccggagagg
      661 cagagcgcgt accgggaacc catcgtccgg gcccattcct gctcgaaacg ctccacctcc
      721 tggaggtggc cctgcttcgg gtccccggtc cggaaaagtt tccggctctc gatgacgcgg
      781 gccaattcct cgatctcttc acgtccgatg cgatacatga cttttctcct tactggttgt
      841 gatccaccta ttcttcaaac caccccggcc tggatgccaa gccgttcgaa tttcccgagc
      901 cagctcgaaa cccgggcctt cgagggagcc gtcaacccgg cggccgggtt ggccaggccg
      961 aggtcgagat acttgccttc gcccaaccgg tggtaaggaa gcagttccac ccgctccacg
     1021 ccgggcaatt cccgggccag cccggccacc agcttgaaaa gttccggccc gacattgaaa
     1081 cccggaacca gcggcacccg cagccagatc cgggcgccgg cccgggccag gcgctccagg
     1141 ttctccagga tcccggcggc cggaacgccg gtctgccggc gatgccggga agcgccggcc
     1201 ttgatgtcga aaaggaaaag gccggtgacc ggaagcagtg cctccagccg atcgaagggt
     1261 ccgaaaccgc aggtttcgac cgcggtgtgc aatccctcgc gccgggccgc cttcaacagg
     1321 gccagggtga aaccagcctg gaagagcggt tcgcccccgg aaaccgtcat gcccccgccg
     1381 gaatattcgt aaaaaagccg gtcccggaga acgtcggaaa cgacttcttt gacttccagc
     1441 cggcggccgg ccaggcgcag ggcgccggtc ggacagcccc cgacgcagcg gccacaggcg
     1501 gcgcaaaggg aacggtcgaa ccgacgcttt ccttcaaccg aatgcgcgcc ccgcgggcag
     1561 gccgcccggc aggcgccgca gccgacgcag aggcggctgg taaaaagcaa aaccggcttc
     1621 ggcgaccagg attccgggtt gtggcaccag cggcagcgga gcggacagcc cttgaggaag
     1681 accgtggtcc ggatgccggg gccgtcgtgc agggaaaacc gctgaatgtc aaaaacgagt
     1741 ccggaagtct ttcccatggt gccgtttctc aaccgccgtg ggcgcgccgc cggatgacgt
     1801 cggcctggac ctccgggctg aggttgacga agaactccga aaaaccgctc acccggacga
     1861 tcaggttccg gtgcttctcc gggtcgcgcc gggccgcctc cagcagttcg ttgccggtaa
     1921 aattgaactg gagctcctgg atgcgccggg ccacgaaaga ccgggtgaag gccgagagca
     1981 ggcgcgttcc ctccggaccg cggaagaagg ccggctcgaa cttcatgttg atcacgctgc
     2041 caccgaccgc accggtgtag tcgggccggg cgaccgaacc gatgaaggcg gtcggcccgc
     2101 gccggtcgcg gccgaagtaa ggcgaggcgg catccgaaag cggagcaccg gcccggcggc
     2161 cgtccggggt ggcgcccacc tcccggccgg ccgggatatt gctgacgttg gccgccatca
     2221 ggccgagaaa gcggccgccg ccggccaacc ggtgttcgag gcaggccgag gtgaagagac
     2281 gcaccaccgc cgcggcgata tcgtccacgt aagaatcgtc attgccgtac ttgggggccc
     2341 ggttcagcag cttcaaacgc aaggcttcgc ccccctggaa atcggcggcc agggccttcc
     2401 gaagcgccgg caggcttact tcgccgcttt caaacaccag ccgccggacc gcggccaggg
     2461 aatcggcaac cgaggccagg cccatgccgg ctatcccgtg aaagcgggga aagaccgcgc
     2521 cgccgtcatt gacgtcccgg ccccggcgca ggcagtcgcg ggtcagggcc gacaggaacg
     2581 ggtcgggaaa cttcccggcc ggaaaccgtt cgatatattc gttgacggac cggacatggc
     2641 cggccagccg tttccggaga accgattcaa agagccgcca gagtttgcgg taggaagaac
     2701 cgggcggcgg gccgaaaagt tccagggctt cggaaaaaca ggccagcagg ttgaagcggc
     2761 tgtcggacca ggccggctgg cggccgggca gcatcgtctc gatgcagccg acgaagcaat
     2821 ggtctcgggc ggccggaagc gggatgccga tccggaccag ggccggaacc aggacttcgt
     2881 cgttgaagat ggccgggaag ccgatcccgg tcttgatcac ttccaggcag gccaggtgga
     2941 atctttccgg ggtggcgtcg tgaaaacggg cgctcaggtt ggcatagggc gtctggacca
     3001 gccgggtcgc ttccaggcaa aggtagctca actcgttgac ggcgtcgcgg ccggccggcg
     3061 tgagtccgcc gatgcagatg ttctggacct ggccgaaccc gtccagcttc acccagagat
     3121 ggcaaagaag atcgagggcc gccggccggg tgatccggcc ggcccggaga tcgcgccggt
     3181 agaaggggta aagatactgg tcaagtcgcc ccagggccat gtgcttacgt ccctcggagg
     3241 caaaggccag atgcgtgagc cagaccagct gcagcgcctc gtggaagctc cggggcggcg
     3301 ccccggcgat ccgattcagg ccggcggcca gctcacgccg gccggaacgt ccggcttcgc
     3361 gcgcgaagcg ccgggccagt ccggagaagg cctccagggt gatgacggcc gattcgagaa
     3421 aatcccgggc cggccccggc cggcgccggt cccgttcccg gcgggcccgg gccgccagcc
     3481 cggcaatgcc aagccggagc aggtccgggt aatgggccag ggtgtggtcg aaaccggccc
     3541 ggaagtcccg ctgcccccgt tcctgatgct cggccaagac cgcctggtac tcggcgcgcc
     3601 cgacgccttc cggaagtttt tccgcaaaga aaaaacgggg atcgccggcc agccgctcgc
     3661 ccggctcgaa ggccgccggc aggttggcca gcaccgaggc gaaggcccgg gcccggaaaa
     3721 tcggctccgg ctccccggcc gaagcgcggt aagccaggac gcgccagtaa ggacgcaggt
     3781 cgaattcgcc gcggcggcgt cttcgggcgc gcccacgcag tttttcccag ttcttccgtt
     3841 ccgtcatcgc tttaaaatcg tc
//
LOCUS       DAOQYN010000182         3842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_24440_length_3842_cov_3.778453, whole genome shotgun sequence.
ACCESSION   DAOQYN010000182 DAOQYN010000000
VERSION     DAOQYN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24440_length_3842_cov_3.778453"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..1674
                     /locus_tag="PKN80_07190"
     CDS             331..1674
                     /locus_tag="PKN80_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNQ35833.1"
                     /translation="MRGGVNLFPSNMGLAATGDPGLAYEVFKAIGRQLSAIGVNFIHS
                     PVLDVNTNPRNPEINFRSFSDDPRLVGEFGVAALKGLAEGDVTAVAKHFPGRGFSAED
                     AHYDLIYYRHNGRQMREIDLLPYRMLIENGLRSVMIAHTVYPGLGDPENPASVSHQVV
                     TRVLREELGFQGIITTDSITMRGLMNKFGLPRACELAFKAGNDLILYRGGVNELFPVI
                     DYLEKAVRKGDLDEGLLNRALARVWGFKEEKGLFARPYIETGKADRPLRDPEIIRLSE
                     TVPARTAILIKKGKAFPLKAKTRVLVAEQLNRFAWQCNDVGYHPGLLYEYLEALADPE
                     RVDVVECTMAGRQDLPRVKERAKAADVIVATSYYCRTVEHNLPFIRKIARLGKPVIVV
                     TNNPYLCKKLMFCDTVLLIFSGNPRGLKAVSQILHEKVRPSGRWPLKDYPRPRPE"
     gene            1671..2579
                     /locus_tag="PKN80_07195"
     CDS             1671..2579
                     /locus_tag="PKN80_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNQ35834.1"
                     /translation="MNGPDPVRNPGKGLAYALAGNLALTIGFICAKISLTAFDPVTFS
                     LVWTATATILTLGYLFLRDGWRKLRLPRELWLRTIAMGAATGAGMIWGWSGLKRLDPS
                     LAAFFGRFETLLIILMGGLFLSERFQRREAAPVALMVAGGFLSATGGGGRVSLGILLI
                     LLAGLATAFQHLLAKGGLDRISPRTMLAYRNGLAFLVVLLWSLSSRLDFQVPAGCWLA
                     TIIGAGFAPFLGFLLVFHSYRHWELSRTGVILNVQPLLVIPASFLVFGTLPGRLALAG
                     GLAILAGSFWLARIHQQVEKQDKAGK"
     gene            complement(2635..3303)
                     /locus_tag="PKN80_07200"
     CDS             complement(2635..3303)
                     /locus_tag="PKN80_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ35835.1"
                     /translation="MALLKPEVAEKVRLLLDQLPSAARLVVFTQELECSHCLQNRQLM
                     EELAGLSGRLSLEVRNFLLESEAVQRYGVDKIPATAVVGDRDRAIRFYGIPAGYEFTS
                     LVEAIRMVAAGDSGLQPETRSRLAALVKPVHIQVFVTLTCPYCPMAVKLAHQFAFESD
                     LVRADMVNIGDFPHLAQRYGVMSVPKVVINETVSVDGALPEPLFLEHLLRTQVPPPEA
                     PIAL"
     gene            complement(3306..>3842)
                     /locus_tag="PKN80_07205"
     CDS             complement(3306..>3842)
                     /locus_tag="PKN80_07205"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ35836.1"
                     /translation="VSFCATCDAPLFEGQTVAVIGGGNSALTAVNDLVRYARKIFVVN
                     IAARIQADPILVEKARAFGRVEFLEEAEVAEIRGRERVEGIRVISRKTGVESDLAVTG
                     VFVEIRLVPNTDFLDGFLALNKQGEVIVDCFCNTSRAGIFAAGDVTTVPEKQIIVAAG
                     EGAKAALTAYKYLLNREG"
BASE COUNT          723 a         1280 c         1161 g          678 t
ORIGIN      
        1 agagaaaatc gggcagtgtt tcaccatcgg gtacaatgac agcatcatca aggccgaagt
       61 ggtcaaggcg gtcaaactcc tcaactgcgg gggactgcgc ctgatcccct gggaaaggga
      121 ggggctggct tacaaaaagg gcgaggaacg gaccttcaag ggcggcgcca tcgacgaaca
      181 gcggggccgg cgcatgggtt cggccgtcta tgccaccccg ggccagtacg cggaactgct
      241 caaccggtat cagtcaatgg cgctcgaacg cgaacacccg gtgccgctcc atactccatc
      301 gaccaggagg gagacagcta taacgactac atccggggcg gagtcaacct ctttcccagc
      361 aacatgggat tggccgccac cggcgacccc gggctggcct acgaggtctt caaggcgatc
      421 ggccggcagc tcagcgccat cggcgtcaat ttcatccatt cgccggtgct tgacgtgaac
      481 accaatccgc gcaacccgga aatcaacttc cgttccttct ccgacgaccc gcgtctggtc
      541 ggcgaattcg gtgtggcggc cctgaagggc ctggccgaag gcgacgtaac ggcagtggcc
      601 aagcacttcc cgggccgggg cttctccgcc gaagacgccc actatgacct gatctactac
      661 cgccacaacg gccgccagat gcgggagata gacctcctcc cctaccggat gctgatcgaa
      721 aacggactgc gctcggtcat gatcgcccat accgtctatc ccggcctggg cgaccccgaa
      781 aacccggcca gcgtctcgca ccaggtggtc acccgggtgc tcagggaaga actcggtttc
      841 cagggcatca tcaccaccga ttccatcacc atgcgcgggc tgatgaacaa gttcgggctg
      901 ccccgggcct gtgaactggc cttcaaggcc ggcaacgacc tcatcctcta ccggggcggg
      961 gtcaacgaac tcttcccggt catcgactac ctggaaaagg ccgtccggaa aggcgacctg
     1021 gacgaggggc tgctgaaccg ggccctggcc cgcgtctggg gtttcaagga ggagaaggga
     1081 ctcttcgccc ggccttacat cgagaccggc aaggccgacc gtccgctccg cgatccggaa
     1141 atcatccgcc tttcggagac ggtcccggcc cggaccgcga tcctgatcaa aaagggcaag
     1201 gccttcccgt tgaaggcgaa gacccgggtc ctggtggccg aacagctgaa ccgtttcgcc
     1261 tggcagtgca acgacgttgg ctaccacccc ggcctgctct acgaatacct ggaagccctg
     1321 gccgaccccg agcgggtcga tgtcgtcgaa tgcaccatgg ccgggaggca ggacctgccg
     1381 cgagtcaagg aacgggcgaa ggcggccgac gtgatcgtgg ccacttctta ctactgccgg
     1441 accgtcgagc acaatcttcc ctttatccgg aagatcgccc ggctgggcaa gccggtcatc
     1501 gtcgtcacca acaacccgta tctctgcaaa aaactgatgt tctgcgacac ggtgctgctg
     1561 atcttctcgg gaaacccgcg gggattgaag gcggtcagcc agatcctgca cgaaaaagtc
     1621 cggccgtccg gccgctggcc gctgaaggat tatccccggc cgcgtccgga atgaacggcc
     1681 cggatccggt tcgaaatccg ggcaaaggcc tggcttacgc gctggccggc aacctggccc
     1741 tgacaatcgg cttcatctgc gccaaaatca gcctgacggc cttcgacccg gtcaccttca
     1801 gcctggtctg gacggcgacc gcaacaattc tgaccctggg ctacctgttt ctccgggacg
     1861 gctggcggaa actccgactg ccacgggaac tctggctccg gaccatcgcc atgggcgcgg
     1921 ccaccggcgc cggcatgatc tggggctggt ccggcctcaa gaggcttgac ccgtcgctgg
     1981 ccgccttctt cggacgattc gaaaccctgc tgatcatcct gatgggcggc ctctttcttt
     2041 cggaacggtt tcagcgccgg gaagcggccc cggtggcgct gatggtagcc gggggattcc
     2101 tgagcgccac cggaggcggt ggccgggttt cgctgggaat ccttctcatc ctgctggccg
     2161 gattggccac cgcctttcag cacctactgg ccaagggcgg cctggaccgg atttctcccc
     2221 gaaccatgct ggcttaccgc aacggcctgg cctttctggt cgtactcctc tggtcccttt
     2281 ccagccggct ggattttcaa gtcccggccg gctgctggct ggccacgatc atcggggccg
     2341 gcttcgctcc ctttctcggc ttcctgctgg tattccattc ctaccgccac tgggaactct
     2401 cccggaccgg cgtgatactc aacgttcagc cgctgctggt aataccggcc tcattcctgg
     2461 tcttcggcac cctgcccggc cgactggccc tggcgggagg actggccatc ctggccggct
     2521 ccttctggct ggcccgtatc caccagcagg tggaaaaaca ggacaaggcg ggaaagtagg
     2581 aacagagcgg gacggccgat aaccgacgcc gccatttggc agtaatccgg ccggctaaag
     2641 ggcgatcggc gcttccgggg gcggaacctg ggtgcgcagc aggtgttcca gaaagagcgg
     2701 ctcgggcagg gcgccgtcga cgctgaccgt ctcattgatt accactttgg gcacgctcat
     2761 caccccgtaa cgctgggcca ggtgcggaaa atcgcctatg ttgaccatgt cggcccgaac
     2821 caggtcgctc tcgaaggcaa actgatgggc cagtttcacc gccatcgggc agtagggaca
     2881 ggtaagggtc acgaacacct ggatgtggac cggcttgacg agtgccgcca gccggctccg
     2941 ggtctccggc tgcaggccgg aatcaccggc ggccaccatc cggatcgcct ccaccagcga
     3001 agtaaattca taaccggccg ggatcccgta aaaacgaatg gcccgatcgc ggtcgccgac
     3061 cacggcggtg gccggaattt tgtccactcc gtaacgctgg accgcctccg actccagcag
     3121 gaaattccgg acctccaggc tcagccggcc ggagagcccg gccagttcct ccatcagttg
     3181 ccggttctga aggcaatggc tgcactccag ctcctgggta aagacgacca gcctcgccgc
     3241 cgacggcagc tgatccagga gcaggcggac tttctcggcc acctcgggtt tcaaaagcgc
     3301 cattctcatc cctcccggtt gagcaggtat ttgtaagcgg tcagggccgc cttggccccc
     3361 tccccggcgg ccacgataat ctgcttctcg gggacggtgg tcacgtcacc ggcggcaaaa
     3421 atgccggccc ggctggtgtt gcagaaacaa tccacgatca cctcgccctg cttgttcaga
     3481 gccaggaatc cgtccaggaa atcggtgttg ggaaccagcc ggatctccac gaaaaccccg
     3541 gtcacggcca ggtcgctttc cacgccggtc ttcctggaaa taactcttat gccttccacc
     3601 cgttcccggc cgcggatttc ggctacctcc gcttcctcga ggaactccac ccggccgaag
     3661 gcgcgcgcct tttccaccag gatcggatcg gcctggatcc gggcggcgat gttgaccacg
     3721 aagatcttcc gggcgtagcg taccaggtcg ttgacggcgg ttaaggccga attgccgccg
     3781 ccgatcaccg ccaccgtctg tccctcaaaa agcggggcgt cgcaggtggc gcaaaaagag
     3841 ac
//
LOCUS       DAOQYN010000183         3840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_24454_length_3840_cov_4.446764, whole genome shotgun sequence.
ACCESSION   DAOQYN010000183 DAOQYN010000000
VERSION     DAOQYN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24454_length_3840_cov_4.446764"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..211
                     /gene="rpmG"
                     /locus_tag="PKN80_07210"
     CDS             62..211
                     /gene="rpmG"
                     /locus_tag="PKN80_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNQ35837.1"
                     /translation="MRELITLECGECHRRNYNSTRDKKQKKERLEARKYCRACRKHTL
                     HRETK"
     gene            241..313
                     /locus_tag="PKN80_07215"
     tRNA            241..313
                     /locus_tag="PKN80_07215"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Trp,seq:cca)
     gene            319..513
                     /gene="secE"
                     /locus_tag="PKN80_07220"
     CDS             319..513
                     /gene="secE"
                     /locus_tag="PKN80_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNQ35838.1"
                     /translation="MEKLILRLRVYFDGVKSEFRKISWPQRKALEQLTAFVLFLVLIL
                     ALFAGILDEFFSRLIRLILG"
     gene            563..1084
                     /gene="nusG"
                     /locus_tag="PKN80_07225"
     CDS             563..1084
                     /gene="nusG"
                     /locus_tag="PKN80_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNQ35839.1"
                     /translation="MTNWFVLHIRSGQEKKVKRYLENLQAKLPDQVKRVLLPTEQVTE
                     MSEGEKKIKSQYFMPGYMILELEDNEEILHEIQTIPGILGFLGDGKNPAPLSEEEVKN
                     ILSLVEERKEKPLLQYEFARGDQVEIRDGPFANFSGLVEEVNSEKEKIKVSVSIFGRS
                     TLVELDVWQVEKV"
     gene            1086..1511
                     /gene="rplK"
                     /locus_tag="PKN80_07230"
     CDS             1086..1511
                     /gene="rplK"
                     /locus_tag="PKN80_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNQ35840.1"
                     /translation="MAKKVKTVVKLQLSAGQATPAPPVGTALGPHGVNIMEFCKQFNE
                     ATRGKEGSIIPAVLTIYEDRSFTFKLKTPPASDLLKKAAGLAKGSGQTGKEIIGSVTR
                     EQVAEIARLKMTDLNASDLEMAVRIIEGTARSLGLEIKE"
     gene            1515..2204
                     /gene="rplA"
                     /locus_tag="PKN80_07235"
     CDS             1515..2204
                     /gene="rplA"
                     /locus_tag="PKN80_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNQ35841.1"
                     /translation="MKKRSKRYQENSRKLTGDKTYTPVEALGLLKEAPAAKFDETVEI
                     SLRLGVDPKKSDQMVRGTAILPHGTGKSIRVLALVADNRMDEAREAGADFVGAQEYIP
                     KIAGGWLDFDVAVTTPDMLREVAKLGKTLGPRGLMPSPKNGTVTTDIGRTIKELKSGR
                     VEFRMDKNGNVHLVIGKISFEPASLLENFNAAMDALLASRPAAVKGAFIKKAYLSTTM
                     SASLPLVLENK"
     gene            2251..2802
                     /gene="rplJ"
                     /locus_tag="PKN80_07240"
     CDS             2251..2802
                     /gene="rplJ"
                     /locus_tag="PKN80_07240"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNQ35842.1"
                     /translation="MAVKTRKKGLSREAKSARVEVLKQEVAEASMVIFTGLTGIPAMD
                     ITEFRKQLRKQGGRLRICKNNVLFRVFDQLEESEIAPLISGSTGLIILKQGDEMLLAR
                     TIYDFLREREEKFMPRGGFQYGMAVSVEQLRMLSKLPGREAVLAQFLGALSGPISNFI
                     YILQAVPQQTVSVLKQISEKEPS"
     gene            2882..3259
                     /gene="rplL"
                     /locus_tag="PKN80_07245"
     CDS             2882..3259
                     /gene="rplL"
                     /locus_tag="PKN80_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNQ35843.1"
                     /translation="MGKDELVEAIAKLTVLELSELTKALEEKFGVSAAMPMMAAGAPA
                     AGAAPAAAAEEKTSFTVILAEAGANKIPVIKEVRAITNLGLKESKDLVEGAPKPVKEN
                     VGKEEAEEIKKKLEAVGAKVEVK"
     gene            3407..>3840
                     /locus_tag="PKN80_07250"
     CDS             3407..>3840
                     /locus_tag="PKN80_07250"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNQ35844.1"
                     /translation="MEAQLDSYRAFLQEGVSPDKRRQDRGLESVFHKIFPLSNNEGTL
                     RLEYSHYLLETPPYTPEECKEKGLTWGSALRVCLRLVKLQKGTGELVQVKEAKEVFLG
                     TIPLMTERCTFVINRGERVIISRLQRSLGVYFKEDNPSPRDYS"
BASE COUNT          901 a         1050 c         1178 g          711 t
ORIGIN      
        1 cgcgagggcg gccacaccgt cggcgccggc gcggtcaccg acgtcatcga gtgagaccaa
       61 catgcgcgaa ctgataaccc tggaatgcgg cgaatgccat cgccggaatt ataattccac
      121 ccgggacaag aaacagaaga aggagcggct ggaagccagg aaatactgcc gcgcctgccg
      181 aaaacatact ttgcaccgcg aaaccaagtg agttttgacg gaggcggaga cgacaaattt
      241 aggcctgtag ctcaattggc agagcaacgg actccaaatc cgtaggttgg gggttcaact
      301 ccctccgggc ctgcgtacat ggaaaaactg attcttagat tgcgggttta cttcgacggc
      361 gtcaagtcgg aattcaggaa gatttcctgg ccccagcgca aggcgctcga gcagctgacg
      421 gctttcgtgc tcttcctggt gctgattctg gcgctttttg ccggtatcct ggacgagttt
      481 ttttcgcgtt tgataaggct catcctggga taaccggccg ttcttttcac cggtatcctt
      541 tgcctgggcc aacgagaccc ttatgactaa ctggtttgtt ctgcacatcc gcagcgggca
      601 ggagaagaaa gtcaagcgct acctggaaaa cctccaggcg aagctgccgg accaggtgaa
      661 gcgcgtgctg ctgcccaccg agcaggtgac cgagatgtcc gagggtgaaa agaagatcaa
      721 gagccagtat ttcatgcccg gttacatgat cctggaactg gaagacaacg aggagattct
      781 gcacgagatc cagacgattc caggcatcct cgggttcctg ggggacggca aaaacccggc
      841 ccccctctcc gaggaggagg tcaagaacat cctttccctg gtggaagaac gcaaggaaaa
      901 gccgctgctg cagtatgaat ttgcccgcgg cgaccaggtt gagataaggg acggcccctt
      961 cgccaacttc agcggcctgg tcgaagaggt caacagcgaa aaagaaaaga taaaggtaag
     1021 cgtttcaatc ttcgggcgtt ctaccctggt ggaattggac gtctggcagg tggagaaggt
     1081 ctaatatggc caagaaagtc aagaccgtgg tgaaattaca gctgtccgcc ggacaagcta
     1141 cgccggcacc cccggtggga accgcgctcg gtccgcacgg ggtgaacatc atggagttct
     1201 gcaagcagtt caacgaggcg acccgcggca aggagggttc catcattccg gccgtcctca
     1261 ccatttacga agaccgcagc ttcaccttca agctgaaaac gccgccggcc tccgacctgc
     1321 ttaaaaaggc ggccgggctg gccaagggca gcggtcagac cggaaaagag atcatcggca
     1381 gtgtgacgcg cgaacaggtc gcggaaattg ccaggctgaa gatgacggac ctcaacgcct
     1441 cggacctgga gatggccgtt cggattatcg agggcaccgc ccggagtctg ggtttggaaa
     1501 tcaaggagta aggcatgaag aagcggtcga agcgttacca ggaaaacagc cggaaattga
     1561 ccggcgacaa gacttacacc ccggtcgagg cgctcggatt gctgaaagag gcgccggcgg
     1621 ccaagtttga cgaaacggtc gagatttcgc ttcgcctggg cgtggatccc aagaagagcg
     1681 accagatggt gcgcggcacc gccatccttc ctcacggcac cggcaagagc atccgggtgc
     1741 tggccctggt ggccgacaac cggatggacg aggcccggga ggccggcgcc gatttcgttg
     1801 gcgcccagga atacatcccc aaaatcgccg gcggctggct tgatttcgac gtggccgtaa
     1861 ccactcccga tatgctgcgg gaagtggcca agctgggtaa aaccctcggt ccgcgcggat
     1921 tgatgccgag tcccaagaac ggcacggtta ccaccgacat cggccggacc atcaaggaac
     1981 tgaaatccgg ccgggtcgag tttcggatgg acaagaacgg caacgttcac ctggtgatcg
     2041 gcaagatttc cttcgaaccg gcctctctgc tggaaaattt caacgccgcc atggacgcct
     2101 tgctggccag ccggccggcc gcggtcaagg gggcgttcat caagaaggcc tacctgagca
     2161 ccaccatgag cgcctccctg cctctcgtcc tggaaaacaa atagtcgttt ttaacataaa
     2221 ctgaaaataa gctaaagaga gggcccgaag atggcagtca agaccaggaa aaaaggcctc
     2281 tcccgggaag cgaagtcggc ccgggtggag gttctgaagc aggaggtggc tgaggcctca
     2341 atggttattt ttaccgggtt gacgggtatc ccggccatgg atatcaccga gttccgcaag
     2401 cagctgcgca agcagggcgg gcggctccga atctgcaaaa acaacgtgct tttccgcgtc
     2461 ttcgaccagc tggaagagtc cgaaatcgcc ccgctgattt cgggttcgac cgggctgatc
     2521 atcctgaagc agggcgacga aatgctcctg gcgcggacga tttacgattt tctccgcgag
     2581 cgcgaggaga aattcatgcc ccggggcggt tttcaatatg ggatggcggt aagtgtcgaa
     2641 caactgcgga tgctttcgaa actgccgggg cgggaagcgg tgctggccca gttcctgggc
     2701 gcgctcagcg gtccgatttc gaactttatt tatattcttc aggccgtgcc tcagcagacg
     2761 gtttcggttc tgaagcagat cagcgagaag gaaccgtcct aaatttccgg ggaatgattt
     2821 ttaaagaact ggaaaatcgc ataacaagtg gaaaggagtc acgatggctg acgaagtgaa
     2881 gttggggaag gacgaactgg ttgaggcgat tgccaagctt acggtgctgg agctttccga
     2941 gttgaccaag gccctcgagg agaagttcgg ggtcagcgcg gccatgccga tgatggccgc
     3001 cggtgccccg gccgccggtg ccgctcccgc ggccgcggcc gaggagaaga ccagctttac
     3061 cgttatcctg gccgaagccg gcgccaacaa gattccggtg atcaaggaag tccgggcgat
     3121 caccaacctc gggctcaagg agtccaagga cctggtcgag ggtgcgccca agccggtcaa
     3181 ggaaaatgtg ggcaaggaag aagccgagga gatcaagaag aagctggagg ccgtcggagc
     3241 caaggtcgaa gtcaagtaaa ccggtccggt tgccatcgcc cgtttccttt ttccggcagt
     3301 cgttcaccct tctcggagtg aaacgagtca gcgtaaattt atgaaaagag cagaatcgtt
     3361 atacggcggg aaaaacaggc tgtccgaacc ggacatcccg aaccttttgg aagcgcagct
     3421 tgattcttac cgagcttttc tccaggaggg tgtctccccg gataaacgcc gccaggaccg
     3481 cggcctcgag tcggtcttcc acaagatatt tcccctcagc aacaacgagg ggacgctgcg
     3541 gctcgaatat tcccattacc tgctggaaac gccgccttac actcccgagg agtgcaaaga
     3601 gaagggattg acctggggtt cggcgctccg ggtttgcctg cggctggtca agctccagaa
     3661 gggaaccggg gaactggtcc aggtcaagga ggccaaggaa gtcttcctgg gcacgattcc
     3721 gctcatgacc gaacgctgca cctttgtgat caacaggggg gaacgggtga tcatctcccg
     3781 cctgcagcgt tcgctggggg tctacttcaa ggaggataac ccctcgcccc gcgattactc
//
LOCUS       DAOQYN010000184         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_24587_length_3826_cov_4.032617, whole genome shotgun sequence.
ACCESSION   DAOQYN010000184 DAOQYN010000000
VERSION     DAOQYN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24587_length_3826_cov_4.032617"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PKN80_07255"
     CDS             <1..802
                     /locus_tag="PKN80_07255"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ35845.1"
                     /translation="VDVDPATLNLDPERLEQALTGRTRAILPVHLYGRPAAMAPIMDF
                     ARRHGLAVIEDACQAHGAELGGVRVPVSGTGCFSFYPSKNLGACGEGGCLVTDSEEVA
                     ARAAALRDHGQTEKYRHQYLGFNYRMPAFQGAVLKVKLPHLEAWTLARREHARRYLEG
                     LAGLDLLRPEDPAGARHVFHLFVVRHPERDRLQADLSGQGIATGLHYPIPVHLQPAFA
                     GRVRPAGPLAVTEAAARQVLSLPMYPELTVEQVDFVCAAMRAALSGHG"
     gene            795..1520
                     /locus_tag="PKN80_07260"
     CDS             795..1520
                     /locus_tag="PKN80_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ35846.1"
                     /translation="MDNPSGVLVFLPVYNEAGKLSGLLEKFRPGLVDEVLVIDDGSTD
                     GSGAVAGRYPVIVLRNEKQRGVGAGIRRAVHYGRQRGHRVLVVMAGNGKDDPREIPRL
                     LEPILREGVDYVQGSRFLSEGRYRNLPLFRLVMIKIFTRLVNLATGFHGTDVTNGFRA
                     YRLGIFDHPAVNIEAEWLDTYEMEYYIHYKALTLGFKVREVPVSKLYPAGRHRAAYTK
                     IRPFVDWWRMVRPLVFLVFRIKR"
     gene            1532..2620
                     /locus_tag="PKN80_07265"
     CDS             1532..2620
                     /locus_tag="PKN80_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015336054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ35847.1"
                     /translation="MAESRREKTMDQIRIDSHKLIYHPERVAAWLAGKDIYPIYLEIG
                     PAGSCNHRCIFCALDYLGYRKNLLETGRLKSFLGEAARKGVKSVMYAGEGEPLLHREI
                     AGIINHTRGLGIDVALTTNGVLFKPELARECLGSLTWMKVSLDAATPETYARVHGCPP
                     GDWRRVMENLKAAVALRAERKYAATIGVQLLLLPENCEEVAELAGQLQAIGADYLVVK
                     PYSQHPKSGHRRPKGFNYARFQKLERELEQFQTDRFQVFFRARAMGKLNRARTYRRCL
                     GLPFWAYLDAAGNLWACSAYLGDRDFLYGNIYQATLGEIWTGRRRREVQARVARMDTG
                     RCREICRLDEINAYLWELKHPSAHVNFI"
     gene            2617..3588
                     /locus_tag="PKN80_07270"
     CDS             2617..3588
                     /locus_tag="PKN80_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNQ35848.1"
                     /translation="MNPTAIPDRVRLSIYRTMLLIRRVEERLCALYPDQEIKCPIHLY
                     IGQEAVAAGVCARLRKSDYLVSNHRSHGHYLAKGGGLREFFAELYGRRTGCSGGWGGS
                     MHLADPELGLLGSSAIVAGGIPIAVGAALAARMEGRGRVAAVFFGDGAADEGVFYESL
                     NFAALRKLPVVFVCENNFYATNSRQSARQPLDNIYRRGRIFGIPGFRLDGNDAAAVYA
                     AAGRAVERARRGRGPTLLECRTYRWKQHVGYQEDWEGGCRPARELSCWKKRCPLERFG
                     RRLRRSGLLTPARERALTAEIDRRIEAARAAARREPSADTIECDGGD"
     gene            3591..>3826
                     /locus_tag="PKN80_07275"
     CDS             3591..>3826
                     /locus_tag="PKN80_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNQ35849.1"
                     /translation="MPWSRIPVDPLETDRAQRLRAEGRRTITYREALREALEQALDTD
                     PRVFLCGEGVDDPGGIFGTTRGLQDRYGRERVFDT"
BASE COUNT          590 a         1265 c         1344 g          627 t
ORIGIN      
        1 ggttgacgtc gacccggcga ccctgaacct ggacccggag cgcctggagc aggcgttgac
       61 cgggcgcacc cgggcgatcc tgccggttca cctttacggc cggccggccg ccatggcgcc
      121 gatcatggat ttcgcccgcc ggcacgggct ggcggtgatc gaggatgcct gccaggccca
      181 cggcgccgaa ctcgggggcg tccgggtgcc ggtctccgga accggctgtt tctccttcta
      241 cccgagcaag aacctgggcg cctgcggcga gggcggctgc ctggtgaccg attcggaaga
      301 ggtcgcggcc cgggcggcgg ccctgcgcga ccacggccag accgagaagt accggcacca
      361 gtacctgggc tttaactacc ggatgccggc cttccagggc gcggttctca aggtcaagct
      421 gccgcacctg gaggcgtgga cgctggcccg gcgcgagcat gcccggcgtt acctggaggg
      481 gttggccggg ctcgacctgc tccggccgga ggacccggcg ggagcgcgcc acgtcttcca
      541 cctcttcgtg gtccgtcatc cggagcggga ccgcctccag gccgacctgt ccgggcaggg
      601 aatcgccacc ggccttcatt acccgatacc ggtccatctc cagccggcct tcgccggtcg
      661 ggtgcggccg gccgggccgc tggccgtgac cgaggccgcc gcccgccagg tgctttcgct
      721 gcccatgtac ccggaactta ccgtcgagca ggtggatttc gtctgcgccg ccatgcgggc
      781 cgccctgtcc ggccatggat aacccgtccg gcgtcctggt cttcctgccg gtctacaacg
      841 aggccgggaa attgagcggc ctgctggaga agttccggcc cgggctggtg gacgaggtgc
      901 tggtgattga cgacggctcc accgacggtt cgggcgcggt cgccggccgg tacccggtga
      961 tcgtcctgcg caacgagaag cagcggggtg tgggtgccgg catccggcgg gccgtccatt
     1021 acgggcgcca acgcggacac cgggtcctgg tggtgatggc cggcaacggc aaggacgacc
     1081 cccgggagat accgcgcctg ctggagccga ttctccgcga gggggtcgat tacgtgcagg
     1141 gatcccgttt cctgtccgaa ggccggtacc ggaacctgcc cctcttccgg ctggtgatga
     1201 tcaagatttt cacccggctg gtcaacctgg cgaccggatt tcacgggacc gacgtcacca
     1261 acgggttccg ggcctaccgg ctggggatct tcgatcaccc ggccgttaac atcgaggcgg
     1321 aatggctgga cacttacgag atggagtatt acatacatta caaggcgctc acgctcggtt
     1381 tcaaggttcg ggaggtcccg gtttccaagc tgtacccggc cgggcgtcac cgggcggcct
     1441 ataccaagat acgacctttc gtcgattggt ggcggatggt ccggccgctg gtttttttgg
     1501 ttttccggat caagcggtga cgggaggctg aatggcggaa tcccggcggg agaagaccat
     1561 ggaccagata cgcattgaca gtcacaagct catctaccat ccggagcggg tggccgcctg
     1621 gctggccgga aaggacatct acccgatcta cctggagatc gggccggccg ggtcctgcaa
     1681 tcaccgctgc atcttctgcg ccctggatta tctcggctac cggaagaact tgctggagac
     1741 cgggcggctc aagtcattcc tgggcgaggc ggcccgcaag ggggtcaaga gcgtgatgta
     1801 cgccggcgaa ggcgagccgc tgctgcaccg ggagatcgcc ggcatcatca accacacccg
     1861 ggggctgggg atcgacgtcg cccttaccac caacggcgtc ctcttcaagc cggagctggc
     1921 cagggagtgt ctcggttccc tgacctggat gaaggtcagc ctggacgcgg ccaccccgga
     1981 gacctacgcc cgggtgcacg gctgtccgcc cggcgactgg cggcgggtca tggagaacct
     2041 gaaggcggcg gtcgccctgc gcgcggaacg gaagtacgcc gccaccatag gcgtgcagct
     2101 gctgctcctg cccgagaatt gcgaggaggt ggccgaactg gccggccagc tccaggccat
     2161 cggggcggac tacctggtgg tcaagcctta ctcccagcac ccgaagagcg gccaccggcg
     2221 tccgaagggt ttcaactacg cgcgtttcca gaagctggag agggaactgg agcagtttca
     2281 gaccgaccgg ttccaggtct tcttccgggc ccgggccatg gggaagctga accgcgcccg
     2341 aacttaccgg cgctgccttg gcctgccgtt ctgggcctat ctcgacgcgg ccggcaatct
     2401 ctgggcctgc agcgcctacc tgggcgaccg ggattttctc tacggcaaca tctaccaggc
     2461 caccctgggc gaaatctgga ccggccggcg ccgccgggag gtccaggcgc gggtggcccg
     2521 gatggacacc ggccgctgcc gtgagatctg ccggctggac gagatcaacg cttatctctg
     2581 ggaactcaag catccctccg cccacgtcaa tttcatatga acccgacggc gattcccgac
     2641 cgcgtccggc tctcgattta ccggacgatg ctcctgatcc ggcgggtgga ggagcggctc
     2701 tgcgcgcttt acccggacca ggagatcaag tgtccgatcc acctttacat cggtcaggag
     2761 gcggtggccg ccggcgtctg tgcccggctg cggaaatcgg attacctggt cagcaatcac
     2821 cggagccacg gtcactacct ggccaagggc ggcggcctcc gggaattttt cgccgaactt
     2881 tacggccggc ggaccggttg cagcggcggc tggggcggtt cgatgcacct ggccgatccg
     2941 gaactgggcc tgctgggcag ttcggccatc gtggccggcg gcattccgat cgcggtcggc
     3001 gcggccctgg ccgcccggat ggaagggcgc ggccgggtgg ccgcggtctt cttcggcgac
     3061 ggcgccgcgg acgagggggt cttttacgag agcctcaatt tcgccgccct gcggaagctg
     3121 ccggtcgtct tcgtctgcga gaacaacttt tacgccacca attcgcgcca gtcggcccgg
     3181 cagccgctgg acaacattta ccgccgcggc cggatcttcg ggatcccggg cttccggctg
     3241 gacgggaacg acgcggcggc cgtttacgcg gcggccggcc gggcggtcga gcgggcccgc
     3301 cggggccggg gtccgacgct gctggagtgc cggacctacc gctggaagca gcacgtcggg
     3361 taccaggagg actgggaagg cggctgccgg ccggcccgtg aactgtcttg ctggaagaaa
     3421 cgctgtccgc tggaaaggtt cggccgccgg ctccggcgct ccggcctgct gacgccggcg
     3481 cgggagcggg cgctgaccgc ggaaatcgac cgccggatcg aggcggcccg ggccgccgcc
     3541 cgccgcgaac cgtcggccga cacgattgaa tgcgacgggg gagattgaag atgccctggt
     3601 cacggatacc ggttgacccg ctggagaccg accgcgccca acggcttcgg gccgaggggc
     3661 ggcggacgat cacctaccgc gaggccctgc gcgaggcgct tgagcaggcc ctggacaccg
     3721 atccgcgggt ctttctctgc ggcgagggcg ttgatgatcc cgggggcatc ttcggcacca
     3781 cccgcggcct ccaggaccgg tatgggcggg aacgggtctt cgatac
//
LOCUS       DAOQYN010000185         3806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_24784_length_3806_cov_5.059717, whole genome shotgun sequence.
ACCESSION   DAOQYN010000185 DAOQYN010000000
VERSION     DAOQYN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24784_length_3806_cov_5.059717"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PKN80_07280"
     CDS             <1..1069
                     /locus_tag="PKN80_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35850.1"
                     /translation="RIINRVLRNHQSLSEPIPDGESAITSLCRGCHGRIYGATSGVRS
                     HLFLYDPGPGGDGVCDIGVIPGATGVRQALVAAPDGTLYGAISEGPEAGRLFRCLTDR
                     DFQNEFGYGCGPVEMLGVPVPGEGIAGLVLDGRRRRFYGVSRASGKFFYYDLQDDRSE
                     VKAELDAKGRFSERLVLDGAGVVYGCRSEGEFFRYDPAVDRLEGLGAHLPALAGREMY
                     NRLESAVFNDYDGLIYGGGSADGVLFSLDPASGRVRSLGKASAAAGCPALAAGFDGRI
                     YGISGTRSGMARLFRYDPDEHSLEDLGLPLAASETFWHGYEFSAACTGSAGEIYLGES
                     DRVSHLFIYFPPVLRRRRADV"
     gene            1095..1901
                     /locus_tag="PKN80_07285"
     CDS             1095..1901
                     /locus_tag="PKN80_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNQ35851.1"
                     /translation="MMDQLSQLNPVIQALAATSFTWGLTALGAALVFLVHRPNQRLFE
                     SMLGMAAGVMIAASIFSLLLPAISLCTDCPYPAWIPAVAGFLLGSVFIMLLDRVLPHL
                     HPGLAVEKAEGIRTKLPTSFLLVLAVTLHNIPEGLAVGVAFGSVSGGEIYRNLASALA
                     IGIGIQNIPEGLAVALPVRNAGQSRLKAFWFGQLSAVVEPVAGVLGAAAVTVSQPLLP
                     YALGFAAGAMMYVVVEEVIPESQAGGHNDLATFSTLLGFLAMVVLDVVVV"
     gene            complement(1914..3647)
                     /locus_tag="PKN80_07290"
     CDS             complement(1914..3647)
                     /locus_tag="PKN80_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ35852.1"
                     /translation="MNEKESSRKNARARRDLAVMAGLAALLFVLESSFGLIGRSVAFI
                     TSRTLTWPLVAFVYVTVCLAFALSIFSLRRWKDLKSEIQERERIEEMLFKIRENLETT
                     IMEQTDDLRKNNRQLMQELAERRRVETALTRSEARFRSFMENGPALAFVKDEAGRYLY
                     VNRAWEKLLGRSRREVEGKTDFEVWPQETAESLQANDRRAWESGQPLEMLETLTLPNG
                     VTVYGLTVKFPLSDPAGGRWLGGMVIDITQMKQEEAARRETEVRYQSIFDNAGDPLII
                     SDLEGKSLEVNRVACDLSGYTREELLQIPITRLIPPKFANRIPRMMKTIKKEGRAVFE
                     LEAVTRDGRSLPLEVSSRLIDFGGRMTILNILRDISERKETEAKLEAGFRKVASMLDD
                     TIHAIGRIVELKDPYTAGHEERVAQLASALARELDLSEEAAGTIYMAGILHDIGKITV
                     PAEILSKPTKLNAYEFSIIQTHAAAGYQILKDIDFPGPIALAILQHHERLDGSGYPNR
                     LPAEKIIREARILTVADVVESMSSHRPYRPAIGLEKALEEITLNRGRLYDPEVVDACL
                     RLFREKSFHFV"
BASE COUNT          553 a         1236 c         1237 g          780 t
ORIGIN      
        1 gcggatcatc aaccgggtcc tgcgcaacca ccagtccctt tccgagccga ttcccgacgg
       61 tgaatcggcc atcacctcgc tctgccgcgg ctgccacggc cggatttacg gagccacctc
      121 cggggtccgg agccatctct ttttatatga tccgggtccg ggcggcgacg gcgtctgcga
      181 tatcggggtg attcccggcg ccacgggcgt gcggcaggcc ctggtggccg ccccggacgg
      241 gacgctttac ggggccatct ccgaggggcc ggaggccgga cgcctcttcc gctgcttgac
      301 cgaccgcgat tttcaaaacg agttcggtta cggctgcggc ccggttgaaa tgctgggcgt
      361 tccggtgccg ggcgagggta tcgccgggct ggtgcttgac gggcggcgcc ggcgttttta
      421 cggcgtgagc cgggcgagcg gaaaattctt ctattacgat cttcaggacg accggtccga
      481 ggttaaggcc gagctggacg ccaaaggacg tttttccgag cggctcgtcc tggatggcgc
      541 cggtgtcgtt tacggctgcc gttccgaggg tgaattcttc cgttacgatc cggcggtcga
      601 ccgcctggaa ggattgggcg cacatctgcc cgccctggcc ggccgggaga tgtataaccg
      661 gctggaatcg gcggttttca acgattacga cggcctgatc tacggcggcg gttcggccga
      721 cggggtcctc ttcagcctcg atcccgcctc cggccgggtc cgttccctgg gcaaggcctc
      781 ggccgcggct ggctgtccgg cgctggccgc cggtttcgac ggccggatct acggaatcag
      841 cggcacccgg tccggcatgg cccgcctctt ccggtacgac ccggacgagc attccctgga
      901 ggatctcggc ctgccgctgg cggccagcga aaccttctgg cacggctacg aattttcggc
      961 cgcctgcacc ggttccgccg gcgagatcta cctgggcgaa agcgaccggg tgagccacct
     1021 cttcatctac ttcccgccgg tactccgccg ccggcgggct gacgtctgac cgggtttcgg
     1081 ccgggaaact cgacatgatg gaccagctat cccagttgaa tccggtcatc caggccctgg
     1141 cggccacctc tttcacctgg ggcctgaccg ccctgggcgc cgccctggtt ttcctggtcc
     1201 accgtcccaa ccaacgcctc ttcgagtcga tgctcggaat ggccgccggg gtgatgatcg
     1261 ccgccagcat cttttccctg ctgctgccgg cgatttcgct gtgcaccgac tgtccttacc
     1321 ccgcctggat cccggccgtg gccggatttc tgctggggag cgtcttcatc atgctcctgg
     1381 accgggtgct gccccacctt caccccggcc tggccgtgga gaaggccgag ggaatccgca
     1441 cgaaactgcc gacctccttc ctgctggtcc tggcggttac ccttcacaac atcccggagg
     1501 ggctggcggt cggggtggct ttcggttccg tctccggcgg ggaaatttac cggaacctgg
     1561 cctcggccct ggcgatcggg atcggcatcc agaacatccc ggagggactg gcggtcgccc
     1621 tgccggtccg caacgccggc cagagccgcc tcaaggcctt ctggttcggt cagctgtcgg
     1681 cggtggtcga accggtggcc ggtgtgctgg gcgccgccgc ggtcaccgtt tcccagccgt
     1741 tgctgcccta tgctctgggc ttcgcggccg gggccatgat gtacgtggtg gtggaggagg
     1801 tgatcccgga gtcccaggcc ggcggccaca acgacctggc cactttcagc accctgctgg
     1861 gtttcctggc gatggtggtc ctggacgtgg ttgtcgttta aggtgggccc ggctcaaacg
     1921 aagtggaagg acttttcccg gaagaggcgc aggcaggcgt caaccacctc cggatcgtac
     1981 aagcggccgc ggttcaaggt aatctcctcc agcgccttct ccaggccgat ggccggccgg
     2041 taggggcggt gcgaggacat cgattccacc acgtcggcca ccgtcaggat gcgcgcctcc
     2101 cggatgatct tttcggccgg gagccggttc gggtagcccg acccgtcaag ccgttcgtgg
     2161 tgctgcagga tggccagagc gatcggtccc gggaagtcaa tgtccttcag gatctggtaa
     2221 ccggcggccg cgtgggtctg gatgattgaa aattcgtagg cgttcagttt cgtgggcttg
     2281 ctgagaattt cggccggcac cgtgatcttg ccgatgtcat gcaggatccc ggccatgtaa
     2341 atggttccgg ccgcctcttc cgaaagatcc agttcccggg ccagcgccga ggccagctgg
     2401 gccacccgtt cctcgtggcc ggcggtgtag gggtccttga gttccacgat gcggccgatg
     2461 gcgtgaatcg tgtcgtccag catcgaggcc accttccgga agcccgcctc cagtttcgcc
     2521 tcggtctcct tgcgctcgct gatgtcgcgg agtatgttga gtatcgtcat ccggccgccg
     2581 aaatcgatca ggcggctgct cacctccagc ggcagcgagc gtccgtcgcg ggtgaccgcc
     2641 tccagttcaa aaaccgcccg gccttctttc ttgatggttt tcatcatccg cgggatccga
     2701 ttggcgaact ttgggggtat caaccgggtg atcggtattt gaagcagttc ctcccgggtg
     2761 tagccggaaa ggtcgcaggc cacccggttg acttccaggg attttccctc gagatcgctg
     2821 atgatcagcg ggtcgccggc gttatcgaag atcgactggt agcgtacctc agtctcccgc
     2881 cgggccgcct cctcctgttt catctgtgtg atatcgatca ccatcccgcc cagccaccgg
     2941 ccgccggcgg ggtcggagag cggaaatttc acggtcagcc cgtaaacggt gacgccgttg
     3001 ggcagcgtaa gcgtctcgag catctcgagc ggttggccgc tttcccaggc ccgccggtcg
     3061 ttggcctgga ggctctcggc cgtctcctgc ggccagactt cgaaatcggt ctttccttcc
     3121 acctcccgcc gggaacggcc cagcagtttc tcccaggctc ggttgacata gaggtaacgg
     3181 ccggcctcgt ccttgacgaa ggccagggcc ggtccgttct ccatgaagga tcggaagcgg
     3241 gcctcgcttc gggtgagggc cgtttcgacc cgccttcgtt ccgccagttc ctgcatcagc
     3301 tggcggttgt ttttgcgcag gtcgtcggtc tgctccatga tggtcgtttc gaggttttcc
     3361 cggatcttga agagcatttc ttcgattcgt tcccgttcct ggatttctga tttcagatcc
     3421 ttccagcggc gcagggagaa gatgctgagg gcgaaggcca ggcagaccgt cacgtaaacg
     3481 aaggcgacca gcggccaggt cagcgtccgg ctggttatga aagccacgct ccggccgatc
     3541 aggccgaagg atgattccag cacgaacagc agggccgcca gtccggccat cacggccagg
     3601 tcgcggcgcg cccgggcgtt tttccgtgag gattcctttt cgttcatgtt ttgtgccggc
     3661 cggtttcagg ccgtcaggat tccgctgccg gccaccggaa aacaggtgaa aaggtccggt
     3721 tccagcccgg ccgggtcgta gtagtcccgg gcgagcgcgg ccgccagttc cccggcccgc
     3781 tcccgttcca ccagggccat gacgca
//
LOCUS       DAOQYN010000186         3788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_24943_length_3788_cov_3.162604, whole genome shotgun sequence.
ACCESSION   DAOQYN010000186 DAOQYN010000000
VERSION     DAOQYN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24943_length_3788_cov_3.162604"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKN80_07295"
     CDS             complement(<1..582)
                     /locus_tag="PKN80_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNQ35853.1"
                     /translation="MKKTPKSLRLQIGLFGRTNVGKSSLLNLVAGQDVAITSPVPGTT
                     TDVVEKSMELLPLGPVVFLDTAGLDDVSVLAEARLRKTRKILDRSDVITLVTEPGAWG
                     DYERRVVREAEARRTPLVAVVNKIDLQAPSADFLEQVAGAAGGRLIACSSLDFANRDR
                     CLNEFKKHLLAVAPDEFLNPPPLLGDLLPPGGLV"
     gene            complement(566..1954)
                     /locus_tag="PKN80_07300"
                     /pseudo
     CDS             complement(566..1954)
                     /locus_tag="PKN80_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
     gene            complement(1951..2217)
                     /locus_tag="PKN80_07305"
     CDS             complement(1951..2217)
                     /locus_tag="PKN80_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HNQ35854.1"
                     /translation="MEKKRLGFVGIIIEDRQKSACKVNEVLSEFGEIIIGRMGLPHPL
                     KKKCCAITLIVDASTDEMGAFTGKLGKIRGVSAKSAMSKETAGK"
     gene            complement(2356..2574)
                     /locus_tag="PKN80_07310"
     CDS             complement(2356..2574)
                     /locus_tag="PKN80_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HNQ35855.1"
                     /translation="MGGGGQPYGVTDERRSKMSRGLYEDDLNQPIRSSHENPQVQQLY
                     REFLGEPRSKKSESLLHVHYKARPQYRK"
     gene            complement(2566..3636)
                     /locus_tag="PKN80_07315"
                     /pseudo
     CDS             complement(2566..3636)
                     /locus_tag="PKN80_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
BASE COUNT          628 a         1276 c         1222 g          662 t
ORIGIN      
        1 caccagtccg cccggcggca gcaggtcgcc gagcaggggc ggcggattca ggaactcgtc
       61 cggcgccacc gccagcaggt gtttcttgaa ctcgttcagg cagcggtcgc ggttggcaaa
      121 atcgaggctg gagcaggcga tcagccggcc gccggcggcg ccggccacct gttccagaaa
      181 atcggccgac ggagcctgga gatcgatctt gttgaccacc gccaccagtg gcgtccggcg
      241 cgcctcggcc tcccggacca cccggcgctc gtaatcgccc caggcgcccg gctcggtgac
      301 cagggtgatg acgtcggacc ggtccaggat tttcctggtc ttgcgcaacc gggcctcggc
      361 caggaccgaa acgtcgtcca ggccggcggt gtccaggaag accaccggac ccagcggcag
      421 cagttccatg ctcttctcca ccacgtcggt ggtggtcccg ggcaccggcg aggtgatggc
      481 cacgtcctgg ccggccacca ggttcagcaa gctcgacttg ccgacgttgg tccggccgaa
      541 gagcccgatc tgcagccgca gcgatttagg cgtctttttc atcttcacgg tgcccgaggg
      601 cggtcaggtt ataagccgaa agggtgcgtt ccaccatttc ccggccgagg cgctcctcca
      661 ggaaggcgcg cagatcgcgg ccgccgccgg cggcgaactc ccgcagcagg gcgccggcgc
      721 gctcgtagcc gagcttgggc agaaaggcgg tgatcagggt ctcgctccgg gccaggatct
      781 ccaggcaccg ctccgggtcg gcctcgacgc cctccaggtg tccggtcagc atccggccgg
      841 cgttcccgag catctccagc gcgtccagca tgacctgcgc caggagcggc agaaattcgt
      901 tgatctgcag cgaggcgcgg ccggccgcct cggtaaccag gaaatcgtcg gcctgggcct
      961 ggagggccgc ctgcatgacc gactccagga tgaccgggtt gaccttgccg ggcatgatcg
     1021 aggagcccac ctggaccgcc ggcagccgga tctcgcccag caggttgagc aggcgcaggt
     1081 cgttgcagac cttgagcagg ttgaccgagt gggccttgag catcccggaa acctcaacga
     1141 acgggtcgct gttggcggtc ggatccaggg tgatctcccc ccggcagagc cccagcccgg
     1201 taagctcgcg cagtttttcg attaccagga atatgtaggc gcgcggcgcg cccagcccgg
     1261 tgccgaccgc ggtaccgccc aggttgacca cccggaggcg ttcctcggcc ttgaagaagc
     1321 gccagcggtc gcgggcgaag gcctcggcga aggcggcaaa ctccgcgccc agcgtgatgg
     1381 gcaccgcctc ctggagctcg gtccgcccga tcttgatcac ctcggcgaat tccttctcct
     1441 tgcgctagca gacgccctgg agccgggcca ccacctggct gagttcccgg aaggccagga
     1501 tggccgccac cttcagggcg gtcggaaagg tgtcgttggt cgactggtga cggttgaccg
     1561 tctccagcgg gtggaccagc ccgtaatcac ccttccggcc gcccaggatt tcaatcgccc
     1621 ggttggccag cacctcgttg acgttcatgt tgaaggaggt gccggccccg ccctggaagg
     1681 cgtccagcgg gaactggtcg gccagcttcc cttcggccag ctcctcgcag gccaggccca
     1741 gggcctggcc gagccgccgg tccaggtagc ccagctcgag gttggccagg cagcaggcct
     1801 tcttgaccag ggccatcgcc cggaccagcc gtcccggaaa gcggcggccg ctgaccggaa
     1861 agttctccat cgcccgctgg gtatggatgc cccagtaagc gtcggcgggc accgccttct
     1921 cgcccaggct gtccttctcc agccgggtgc tcatttcccg gccgtctcct tgctcatggc
     1981 cgacttggcc gaaacgcccc tgatcttgcc cagcttgcca gtgaaggcgc ccatctcgtc
     2041 ggtgctggca tccacgatca gggtgatggc acagcacttc ttcttcaagg gatggggcag
     2101 ccccatccgg ccgatgatga tctccccgaa ctccgagagg acctcgttga ccttgcaggc
     2161 gctcttctgc cggtcctcga tgataatgcc cacaaatccc aaccgcttct tctccattcc
     2221 ccgcctcctt tcaagaaaaa aacccagccg gcggcctggc cgcggtcggc tgggttattc
     2281 tttcttaccc acctcccttg gcctcagagc cgctggctcc tcacctcagg acaactgttt
     2341 caaccttatt ataccctatt tccggtactg gggtcgcgcc ttgtaatgaa cgtgcagcag
     2401 ggactcggat ttcttgctcc ggggctcgcc caggaactcg cggtaaagct gctggacctg
     2461 agggttctca tgcgaggagc ggatgggctg gttcaggtcg tcctcgtaca ggccgcgcga
     2521 catcttgctg cgccgctcgt cggtcacccc gtagggctgc ccgccgccgc ccacgcagcc
     2581 gcccgggcag gccatcactt ccacgaaatg gtagggtacc ggccggccgg ccttgacggc
     2641 ctcccggacc tggttgagca cgtactccac gttgctcagg ccgtgggcga ccgcgatgcc
     2701 gaccctggtt ccggcgatct cgatcgaact ctccttgact cccttaagcc cgcgcgtctc
     2761 ggagaactcc agccggtcca gcttctgacc ggtgatgtag aagcaggccg tccggagggc
     2821 cgcctccatc acgccgccgg tggccccgaa aatcacgccg gcgccggtat aattcccgag
     2881 gatatggtcc ggttcctcct cgggcaggga cggaaaatca atcccggcct gcttgatcat
     2941 ccgggccagc tcacgggtgg tcagcacgat atcgatgtcc tggaagccgg aggcgaacat
     3001 ctcgtcggtg cgggttatct cgtacttctt ggcggtgcag ggcatgatcg aaaccatcac
     3061 gatccgggcc gggtccaggt tgtacttgct ggcgtagtag gtcttggaga gcacccccag
     3121 gatctggtgc ggcgacttgc aggaggagaa gtgggggatc atgtcgtggt ggaacttctc
     3181 catgaaatcg acccaggagg ggcagcaggt ggtgatcagg ggcagcgggc gctccgggct
     3241 cgggttcttg aagcggtcca cgaactcgct ggcctcctcg atgatggtca cgtcggcccc
     3301 gaagttggtg tcgaagacgg ccgcgaagcc gagccggcgg agcagggcgt aaagccggcg
     3361 cgtgaagtta tccccgggct ggaagccgaa ggcctccccg atcgacaccc ggaccgccgg
     3421 cgcgatctgg accacgcagt agttgccggg cttctggagg gcctcccaga cccgggcggt
     3481 gtcatcctgt tcgaagatgg ccccggtcgg gcagtgggcc gaacactggc cgcagcgcac
     3541 gcagggcgat tcgcccagct tgatgtcgcc ggccggcgag atccgggtct cgaaaccccg
     3601 ctccaggaag gagagggccc agacatcctg catctgctgg cagactttgt attgttcaag
     3661 tcggccggcg acagtgtggt agccatcagt tcaggcttta ccattggcaa gcagccagcg
     3721 ccaactccac ccattaccaa accggacaac ctgcgcctgc tgcagggaga aaaccccggc
     3781 gaactggt
//
LOCUS       DAOQYN010000187         3777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_25049_length_3777_cov_5.100215, whole genome shotgun sequence.
ACCESSION   DAOQYN010000187 DAOQYN010000000
VERSION     DAOQYN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25049_length_3777_cov_5.100215"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PKN80_07320"
     CDS             <1..946
                     /locus_tag="PKN80_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ35856.1"
                     /translation="LLSGRIFVDATGDADLAAWSGLPFQKGRPEDGRGQSMGSIFRVA
                     GIDEARLTPEVRREAHARLKELRDRGELTIYNQSLGGKGSTIRPGEHTFNLTRFPGDA
                     TRTAELTAAELFLRRETHRIVEFLRRNVAGFEKSYLAASPANAGVRETRQIEGLATLS
                     AGDVLEGRKSETSIARCAYCIDIHCPLGYTTGNIHYCHQDCPSTLPCPMLEKYRDRLP
                     KTSETGMSSLRPPAGDWFNIPYGCLVPRGSRNLLVAGRCISATHQAMSSIRVMATCMA
                     TGQAAGIAAALAARGRIPVSEVPVSEIQARLREQGALY"
     gene            972..1643
                     /locus_tag="PKN80_07325"
     CDS             972..1643
                     /locus_tag="PKN80_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35857.1"
                     /translation="MDCRRLDPESSAPEAAAFFLPVAAAGRRRFGDPFQAAADRRLAE
                     RYRITCLFPDFPEMPWYADRPDGTVRQESRLLDLVNGRVSGVPRAAPRRRLLIGFSKS
                     GWGAAAFLFRHPESVAAAAAWDAPFLYDSLSDPEMETAFGDETNLRRYRLLDLAETAD
                     PELKERPRLLLAGGDLFLPDLEAARRKLLETGVPHLYRDDLRHPHRWDGGWLEEVFRL
                     TLSLL"
     gene            1736..2443
                     /locus_tag="PKN80_07330"
     CDS             1736..2443
                     /locus_tag="PKN80_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ35858.1"
                     /translation="MNNIEAILTERIRRTGTVESFRFTTGEPFAFLPGQFLKVNFAPD
                     RNDKELNKFLSFSSAPGRSYLEFTKRLSESVFSRRLAALKPGDRVQIQGPLGNCVFQD
                     DYREITFLIGGIGITPVISIIEHVMEKRLDTRINLFYSNRTAEEIAFKAELDAWAAAN
                     PNLRIIYTITGEAAHDPRFFSGYINQELVSGNLRAIQDQVFFIYGPPSMTWTMKQLCS
                     EIGCVPGRVKTENFVGY"
     gene            complement(2834..2905)
                     /locus_tag="PKN80_07335"
     tRNA            complement(2834..2905)
                     /locus_tag="PKN80_07335"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2871..2873),aa:Ile,seq:cat)
     gene            3193..3462
                     /locus_tag="PKN80_07340"
     CDS             3193..3462
                     /locus_tag="PKN80_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNQ35859.1"
                     /translation="MNKAELVNAVAKAVATKKDAAAAVDATLDAITASMKKGQAVTLV
                     GFGTFKVVRRKARTGRNPRTGRTITIAAKKVPVFKAGSALKSAVK"
     gene            complement(3564..>3777)
                     /locus_tag="PKN80_07345"
     CDS             complement(3564..>3777)
                     /locus_tag="PKN80_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35860.1"
                     /translation="IQQAGETTPEYYVRELKRWPLVILGTRIRVESPPPRTELEFMVR
                     YKEGNLADQVVPHLMKLPGVKLVEWK"
BASE COUNT          727 a         1288 c         1092 g          670 t
ORIGIN      
        1 cctcctatca ggccggatct tcgtcgacgc caccggcgac gccgacctgg ccgcctggag
       61 cggtctaccc tttcaaaagg gacgcccaga agacggccgc ggccagtcga tgggcagtat
      121 cttccgggtc gccggcattg acgaggcgcg cctgacgccc gaggtccgcc gggaggcgca
      181 cgcccgcctg aaggagctgc gcgaccgggg cgaactgacc atctacaacc agtccctggg
      241 cggcaagggc agcaccatcc ggccgggcga acataccttc aacctgaccc ggtttccggg
      301 cgacgccacc cggaccgccg agctgaccgc cgccgagctc ttcctgcggc gggagaccca
      361 ccggatcgtc gagttcctgc gccggaacgt ggccggcttc gaaaagagct acctggccgc
      421 cagcccggcc aacgccggcg tgcgggaaac ccgccagatc gaggggctgg ccaccctctc
      481 ggccggggac gtgcttgagg gacgcaagtc ggagacgagc attgcccgtt gcgcttactg
      541 catcgacatc cactgcccgc tcggctacac caccgggaat atccattact gccaccagga
      601 ctgtccgtcc acgctgccct gcccgatgct ggaaaagtac cgggaccggc tgccgaagac
      661 ttccgaaacg ggcatgagca gcctccggcc gcccgccggc gactggttca acattcctta
      721 cggttgcctg gtcccacgcg gcagccgcaa cctcctggtc gccggccgct gcatttcggc
      781 cactcaccag gcgatgtcct ccatccgggt catggccacc tgcatggcga ccggccaggc
      841 cgccggcatc gcggccgccc tggcggcccg gggccggatt ccggtctccg aggtgccggt
      901 ctccgaaatc caggcccggc tccgggagca gggcgccctt tactgaaaaa agaccgcgcc
      961 gggggctgaa gatggactgc cgccgcctcg atccggaatc ctccgccccg gaagccgccg
     1021 ccttcttcct gcccgtggcg gcggccggcc gccgccggtt cggcgacccc tttcaagccg
     1081 ccgccgaccg gcgcctggcg gaacgctacc ggataacctg cctcttcccg gacttcccgg
     1141 agatgccctg gtacgccgac cggcccgacg gcaccgtccg ccaggaaagc cgactgctcg
     1201 acctggtcaa cggccgggtc tccggcgttc cgcgggccgc gccgcggcgc cggcttctga
     1261 tcggcttcag caaatccggc tggggcgcgg ccgcctttct cttccgccat ccggaatcgg
     1321 tcgcggcggc cgccgcctgg gacgcgccct ttctctacga cagcctctca gacccggaaa
     1381 tggaaacggc cttcggcgat gaaaccaacc tgagacgcta ccggctgctg gacctggccg
     1441 aaacggccga cccggaactt aaagaacggc cgcgtctgct cctggccggc ggcgatcttt
     1501 ttctacccga tctcgaggcc gcccgccgga agctgctcga aacgggcgtc ccccaccttt
     1561 accgggatga cctccgccac ccgcaccgtt gggacggcgg ctggctggaa gaggtgttcc
     1621 gcctcaccct ttccctgctg taaaccggcc gggaacttga ccggaacgaa catctccggc
     1681 ataatatctt cccgaaagcc gacagcccat tatattcata accaggcgag gcgcggtgaa
     1741 taatatcgaa gcgatactga ccgaacgcat ccggcggacg ggcaccgtcg agagcttccg
     1801 tttcacgacc ggggagccct ttgcctttct gcccggccag ttcctcaagg tcaacttcgc
     1861 cccggaccgc aacgacaagg agctcaacaa attcctctcc ttctccagcg cgcccggccg
     1921 ctcttatctc gaattcacca agcggttgag cgaaagcgtc ttctcccggc gcctggccgc
     1981 cctcaagccc ggcgaccggg tccagatcca ggggccgctc ggcaactgcg tcttccagga
     2041 tgattaccgc gaaatcacct ttctcatcgg cggcatcggt atcacgccgg tcatctccat
     2101 catcgagcac gtcatggaga aacggctgga tacgcgcatc aacctcttct actccaaccg
     2161 gaccgctgag gagatcgcct tcaaggccga gctggacgcc tgggcggcgg ccaacccgaa
     2221 cctgcggatc atctacacca tcaccggcga ggcggcgcac gacccccgct tcttcagcgg
     2281 atatatcaac caggaactgg tcagcggcaa ccttagggcc atccaggacc aggtcttctt
     2341 catctacggc ccgccttcca tgacctggac catgaaacag ctctgcagcg aaatcggctg
     2401 cgttcccggc cgggtcaaaa ccgagaactt cgttggttat tgaagcgcgg aaatgagttg
     2461 gaagggtata caggaagctt aaaagtgggc ccagcaggac gggatcggcg ccctcgcctc
     2521 ccactgcggc tcgggtcggc cattcgctgg tttgtctgaa taagattcag aaacaataat
     2581 cgccatgaag cagagcgaag cacgcttcat ggccgtgaag tgtgcgctga gctcggtcga
     2641 agcgctctac ttcacggctc tcttcgatcc ctgctttgta caagaagcgt aaaagtgggc
     2701 ccagcaggac gggatcggcg ccctcgcctc ccactgcggc tcgggtcggc cacccgctgg
     2761 caacgctcct taatcagtcg cgtcacctga cttcgctctc ttcgatccct gctttgtaca
     2821 agaagcgtaa aagtgggccc agcagggatc gaacctgcga ccaagggatt atgagtcccc
     2881 tgctctgccg ctgagctatg ggccctggct attattatac tacaccgccg gccggatccg
     2941 gccgttttta agatggaaaa ccggcccttt taaaaatcaa aacggtccgg ccgtttttac
     3001 cttgactggc aagcgatgcg ctggtatgcc cccgcctatt cccttgtcct gattgctttc
     3061 caggaaatca acccgccgtt tttcctttgc ccacttgaca aaaaccgtat cagcaggtac
     3121 aataccttta aaatcgaatt tccgggttcg caagaccctg gaaaattcaa aaaagaaagg
     3181 gggtgaattt aaatgaacaa ggctgagctg gtaaatgccg tcgccaaggc cgtcgccacg
     3241 aagaaggacg ccgccgccgc cgttgatgcc accctggatg ctatcaccgc ttcgatgaag
     3301 aaaggtcagg ccgtcaccct ggtcggtttt ggaaccttca aggtcgtaag gagaaaggcc
     3361 cgcaccggtc gcaacccgcg cacgggcagg acgatcacca tcgccgccaa gaaggtccct
     3421 gtcttcaagg ctggcagcgc gctgaagtcc gctgtgaagt aagattttaa aaaaggaccg
     3481 ggcaggttga aaataacgca acctgcccgg tttcctgttt cagggaagaa agccacgacc
     3541 ggaatcccgg gccgcggcct tttttatttc cactccacca gcttcacccc cggcagcttc
     3601 atcaggtggg gaacaacctg gtcggccagg ttgccctcct tgtagcggac catgaactcc
     3661 agctcggtgc gcggcggcgg cgattcgacc cggatccggg tgccgaggat gaccagcggc
     3721 cagcgcttga gttccctgac atagtattcc ggggtggtct cgccggcctg ctggatg
//
LOCUS       DAOQYN010000188         3723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_25619_length_3723_cov_4.863686, whole genome shotgun sequence.
ACCESSION   DAOQYN010000188 DAOQYN010000000
VERSION     DAOQYN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25619_length_3723_cov_4.863686"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PKN80_07350"
     CDS             complement(<1..455)
                     /locus_tag="PKN80_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNQ35861.1"
                     /translation="MTRRYDYHSRVEPIALARSGRAASYPPAEKIRIIEVPDFPSLGK
                     LTALRFLEWLQLNPEGVVSLPTGKTPEYFIKWTGYYLEAWKEAGVQKELAAWGLDPKQ
                     RPRMEAVTFVQIDEFYPINPQQKNSFNHYIQKFYVKGFGLDRDRVILMDT"
     gene            complement(452..1426)
                     /locus_tag="PKN80_07355"
     CDS             complement(452..1426)
                     /locus_tag="PKN80_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35862.1"
                     /translation="MTGRELVRRAARFQGPERVPRFLPEPWGNDFFGLGAGVDPDFKP
                     RVEGEDEFGCVWVKLPGDRTMGQVKVHPLSDYRRLADYRFPDYTRPGRYQPARDRIKE
                     NREERFVLFNTPLSLIHRLEYLRGHVEAYTDPYLYPKELEFLLDHLTDISIDAIRHGA
                     EIGVDGIISCDDWGLQDRPMLSPEIFRRFFKPRYARAYGEAHRLGLVTFLHSCGHIVE
                     LLDDLIESGLDVIQMDQQENMGLDLLSRRFGGRLCFWCPVDIQQTMIKGSPAEVAAYA
                     RRLIYTFGNYNGGFISKWYNSPQAVNHDPVKIEAMARSFVENGNYPLK"
     gene            1726..1986
                     /locus_tag="PKN80_07360"
     CDS             1726..1986
                     /locus_tag="PKN80_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNQ35863.1"
                     /translation="MKQTEVETRVKQVVARLLKVPEAEIKSESDFVKDLGAESIQSLE
                     LVASFESEFEIEMDEDAALAVKTVGSAVEFISRILQTEGRLD"
     gene            2002..2817
                     /gene="nagB"
                     /locus_tag="PKN80_07365"
     CDS             2002..2817
                     /gene="nagB"
                     /locus_tag="PKN80_07365"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNQ35864.1"
                     /translation="MQIIIKTGVPEMSRQAARIVKARLQEKPDLVLGLATGSTPLGLY
                     QELIRLHREEGLDFSRVTTFNLDEYLGLPPEHPQSYNYFMWEHLFSHINIRKKNVHLP
                     DGRTPYSRTAAYCREYEERIVKAGGIDLQILGIGRDGHIGFNEPGSALASRTHVKTLT
                     EETIQDNARFFADPGQVPLYAITMGVGTVMDAREIILLANGANKADAIYKAIEDGITC
                     QVTASILQFHPKATFIIDEEAASLLQRKSYYKFVAQAVEEFERRAAGECRPGK"
     gene            2822..>3723
                     /locus_tag="PKN80_07370"
     CDS             2822..>3723
                     /locus_tag="PKN80_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNQ35865.1"
                     /translation="MNLVEDFKEKARRSPRRIVFPEAGDERILAAALELDREGIAVPV
                     LVGDRRKIAETAAAAGLADPAGRLEIREPKSDPGLERYVDSYLAKRPLAREVARKLVA
                     KNLAFGSLLVKEGEADGLVAGAEHPTATIIQATLTTIGTAPGISTLSSCFLMLLPDFL
                     GEKDRVLVYADAGVNVDPTPDQLADIAVASGRTARKLLGVEPRVALLSFSSKGSAAHP
                     MVDRVIQAVEAARRKDPSLLIDGELQADTALVPRVAAKKLKEPGPVAGRANVLVFPDL
                     NAGNIAYKLTQYLAGASAYGPLLQG"
BASE COUNT          703 a         1203 c         1185 g          632 t
ORIGIN      
        1 gtatccatca ggatgacccg gtcccgatcc aggccgaaac ccttgacgta gaacttctgg
       61 atgtagtggt tgaaactgtt cttctgctgg gggttgatcg ggtagaactc gtcgatctgg
      121 acgaaggtga ccgcctccat ccggggccgc tgtttcggat ccagtcccca ggcggccagt
      181 tccttctgga ccccggcctc tttccaggcc tccaggtagt agccggtcca cttgatgaag
      241 tactccgggg tcttgccggt gggcagggag accacgcctt cgggattgag ctgcagccac
      301 tccaggaaac gcagggcggt caatttcccc aggctgggaa agtcgggcac ctcgatgatc
      361 cggatcttct cggccggcgg gtagctggcc gcccggcccg agcgggccag ggcgatcggc
      421 tccacccggg aatggtagtc gtagcgtctg gtcatttcag cgggtaattg ccgttttcga
      481 cgaaagagcg ggccatggcc tcgatcttga ccgggtcgtg gttgaccgcc tgcggcgagt
      541 tgtaccactt ggagatgaag ccgccgttgt aattcccgaa ggtgtagatg aggcgccggg
      601 cgtaggcggc cacctcggcg gggctgccct tgatcatcgt ctgctggata tcgaccgggc
      661 accagaagca gaggcggccg ccaaaacgcc tggaaagcag atccaggccc atgttctcct
      721 gctggtccat ctggatgacg tccagcccgg actcgatcag gtcgtcaagc agctcgacga
      781 tgtggccgca gctgtgcagg aaggtgacca gtccgagccg gtgcgcctcg ccgtaggccc
      841 gggcataacg cggcttgaag aagcggcgaa agatctccgg cgagagcatc ggccggtcct
      901 ggaggcccca gtcgtcgcag gagatgatgc cgtcgacgcc gatttcggca ccgtgccgga
      961 tggcgtcaat ggagatatcg gtcaggtggt cgagcaggaa ttccagttct ttcgggtaaa
     1021 ggtaggggtc ggtataggcc tcgacgtggc cgcgcaggta ctccagccgg tggatcaggc
     1081 tcaagggggt gttaaagagg acgaagcgtt cttcccgatt ctccttgatc cgatcccggg
     1141 ccggctggta gcggcccggc cgggtgtaat cggggaaccg gtagtcggcc agccgccggt
     1201 aatcggagag cgggtggacc ttgacctggc ccatggtccg gtcgccgggc agcttcaccc
     1261 agacgcagcc gaattcgtcc tcgccctcga cccgcggctt aaaatcagga tcgacgccgg
     1321 cgccgaggcc gaagaaatca ttgccccagg gttcgggcag aaagcgcggc acccgctccg
     1381 gcccctggaa acgggccgcc cgccgcacca gctcccggcc ggtcacgctc ccctccccgg
     1441 ccggcctctg agtaacgccg tccgccattt atccgacatt ggttccactc ctggtttgat
     1501 ttccggtgcc tataaattaa ttttaccacg ccggtcaacc gccggacccg gccggtgcgt
     1561 cccggccgct tctttgacaa accggctgga caggtggtat tttaaaattt cttgaaatac
     1621 taagcgcgtc cggtcggtct aatgatacaa aaaaggtggc gcgcgccgct cgtcaccttg
     1681 atgaatccac ccactttttc tttttccgac cgaactggag gaaagatgaa gcagaccgaa
     1741 gtggaaacga gggtgaaaca ggtagtggcc cggctgctga aggtgccgga ggccgaaatc
     1801 aagtcggagt cggattttgt gaaagacctg ggtgcggagt cgatccagag cctggaactg
     1861 gtggcctcct ttgagagcga gttcgaaatc gagatggatg aagatgccgc cctggcggtc
     1921 aaaacggtcg gttccgcggt cgaattcatc agccggattc tccagaccga aggccggctg
     1981 gactaactaa cgggaggcgc gatgcagatc atcatcaaaa ccggcgtgcc ggaaatgagc
     2041 cgccaggcgg cccggatcgt caaggcccgc ctgcaggaaa aaccggacct ggtcctcggc
     2101 ctggccaccg gcagcacccc gctcggcctt taccaggaac tcatccgcct gcaccgggaa
     2161 gagggcctgg atttttcgcg ggtgacgacc ttcaacctcg acgagtacct cggcctgccg
     2221 cccgaacacc cccagagtta caactacttc atgtgggagc acctcttcag ccacatcaac
     2281 atccgaaaga agaacgtcca ccttccggac ggccgcaccc cgtacagccg gaccgccgca
     2341 tactgccgcg agtacgagga gcggatcgtc aaggccggcg gcatcgacct ccagatactg
     2401 ggcatcggcc gcgacggcca tatcggcttc aacgagcccg gttcggccct ggcctcgcgc
     2461 acccatgtca agaccctcac cgaggagacc atccaggaca acgcccgctt cttcgccgac
     2521 cccggccagg tgccgcttta cgccatcacc atgggtgtgg gaacggtcat ggacgcccgg
     2581 gaaatcatcc tgctggccaa cggcgccaac aaggccgacg ccatctacaa ggccatcgaa
     2641 gacggtatca cctgccaggt gaccgcctcc atcctccagt tccacccgaa ggccaccttc
     2701 atcatcgacg aggaggccgc cagcctgctt cagcgcaaga gctactacaa gttcgtcgcc
     2761 caggcggtcg aagagttcga acggcgggcc gccggggaat gccgcccggg gaaataggtc
     2821 catgaacctg gtcgaagatt tcaaggagaa ggcccgccgt tcgccccggc ggatcgtctt
     2881 tccggaggcc ggggacgaac ggatcctggc cgccgccctg gaactggacc gggaaggaat
     2941 cgcggtgccg gtgctggtcg gcgaccgtcg caagatagcc gaaacggccg ccgcggccgg
     3001 actggccgac ccggccggcc ggctggaaat ccgggaaccg aaatcggatc cgggtctgga
     3061 acggtacgtg gattcctacc tggccaagcg gccgctggcc agggaggtgg cccgcaagct
     3121 ggtggccaag aacctagcct tcggctcgct gctggtcaag gagggcgagg ccgacgggct
     3181 ggtggccggg gccgagcatc ccaccgccac catcatccag gccacgctga ccaccatcgg
     3241 caccgctccc ggcatctcaa ccctctccag ctgcttcctg atgctcctgc ctgatttcct
     3301 gggcgaaaag gaccgggtcc tggtttacgc cgacgccggc gttaatgtcg accccacacc
     3361 cgatcagctg gccgatatcg ccgtggcctc gggccggacg gcccggaagc tgctgggagt
     3421 ggaaccgcgg gtcgcgctgc tctccttctc ttccaaggga agcgccgccc acccgatggt
     3481 ggaccgcgtc atccaggcgg tcgaggcggc ccggcggaag gacccgtccc tcctgatcga
     3541 cggcgagctg caggccgaca ccgccctggt gccgcgggtg gccgccaaga aactcaagga
     3601 gccgggcccg gtggccggcc gcgccaacgt gctggtcttc ccggacctga acgccggcaa
     3661 catcgcctac aagctgaccc agtacctggc cggcgccagc gcctacgggc cgctcctgca
     3721 ggg
//
LOCUS       DAOQYN010000189         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_25880_length_3697_cov_5.115047, whole genome shotgun sequence.
ACCESSION   DAOQYN010000189 DAOQYN010000000
VERSION     DAOQYN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25880_length_3697_cov_5.115047"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2545)
                     /gene="secA"
                     /locus_tag="PKN80_07375"
     CDS             complement(<1..2545)
                     /gene="secA"
                     /locus_tag="PKN80_07375"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNQ35866.1"
                     /translation="MADFQHILDEARKNRSGLLARFMKCKSERDITRLLPRLAEVNRL
                     EPELLNLSDEQLAARTGEFRERLERGATLEDLLPEAFATVRESARRRLNMRHFDVQIL
                     GGIALFQGNIAEMATGEGKTLVATLPLYLAALEGKGAHLVTVNDYLAKRDRFWMGPVY
                     ESLGLSVGTIQHDSSREERQAAYNSDITYGTNNEFGFDYLRDNMALRGEDRVQRPLNY
                     AIVDEVDSILIDEARTPLIISGPAEESTALYYEIDKVFRRLDTKSDYEWDEKNGSGFL
                     TEAGIRHAEKILNIENLYDAQHIQLVHHLNQALRAHRGFERDREYLVKDGKVVIVDEF
                     TGRMMEGRRYSDGLHQALEAKEGVKIEAENQTLATITFQNYFRLYRRLGGMTGTAATE
                     AVEFLQIYNLDVVSIPPNRPLIRVNHPDAIYKTAQEKYKAAVQEIEELYRRGQPVLVG
                     TVSIEKSELLSRMLTRRNIPHQVLNAKYHEKEAAIVAEAGRKYKVTIATNMAGRGTDI
                     ILGGSQSGREDWTDEHRRKVEAIQAAFPGVSPGQLEGKSLEGLEVVELGGLHILGTER
                     HEARRIDNQLRGRSGRQGDPGSSRFYLSLEDDLMRLFGSERLLGVMNALPDGEPISHP
                     LISRLIENAQRRVEGQNFDIRKQLLDFDNVMNEQRLVIYRMRNQILDGADLTEHLQSL
                     FLEEVADGLDRFCPPQVRPEEWTRAEFGRWFESVFNAPPEPFQEADLVRSRENVAGSL
                     TAAVGRAYQERRQRLGTELAAEMERHFYLFVLDSRWKAHLHDMDHLREGISLRAYGHQ
                     DPLIAYKSESFALFQEMQDEVRRQLVGLLFRVETAPAGPAPRLVLTPTSY"
     gene            complement(2562..3341)
                     /locus_tag="PKN80_07380"
     CDS             complement(2562..3341)
                     /locus_tag="PKN80_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNQ35867.1"
                     /translation="MLFDTHCHLNFPDFDSDRSEVVRRARDNGVVEILVVGTDPSGNR
                     KAIEVAGEFGLRAALGFHPGHAEEAEPDLAEFRRALADPRVVAIGEIGLDYHYQVDRD
                     KQAAVFRRMLDLAVEAGRPVIVHSREAAGPTLAALATAGVKRGVWHCFSGDVRLAGQV
                     LELGLEISFTANLTYRRNEALREVARQVPLERLLLETDAPFLPPEGKSGRRNEPAFLP
                     ALAGALAEIKGVRPEAVAETTFEAGRRLFRNGRFDKDTSEV"
     gene            complement(3394..3466)
                     /locus_tag="PKN80_07385"
     tRNA            complement(3394..3466)
                     /locus_tag="PKN80_07385"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3431..3433),aa:Phe,seq:gaa)
BASE COUNT          553 a         1209 c         1250 g          685 t
ORIGIN      
        1 ggtagctggt gggtgtcagg accagccgcg gcgccgggcc ggccggggcc gtctcgacgc
       61 ggaagaggag gccgaccagc tggcggcgca cctcgtcctg catctcctgg aagagcgcga
      121 agctctcgct cttgtaggcg atgagcggat cctggtgccc gtaagcgcgc agcgaaatgc
      181 cttcgcgcag gtggtccatg tcgtgcaggt gggccttcca gcggctgtcc agcacgaaaa
      241 ggtagaagtg gcgctccatc tcggcggcga gctcggttcc cagccgctgg cgccgctcct
      301 ggtacgcccg tccgaccgcc gcggtcaggc tgccggccac gttctcgcgg gagcgcacca
      361 ggtcggcctc ctggaagggc tccggcggcg cgttgaagac cgattcaaac cagcggccga
      421 actcggcccg ggtccactcc tccggcctta cctgcggcgg gcagaagcgg tccagcccgt
      481 cggcgacctc ctccaggaaa agagactgaa ggtgctcggt caggtcggct ccgtcgagga
      541 tctggttgcg catccggtag atgaccaggc gctgttcgtt catcacattg tcgaagtcca
      601 gcagctgctt gcggatatcg aagttctgcc cctcgacgcg gcgctgcgcg ttttcgatca
      661 gccggctgat gagcgggtgc gagatcggct ctccgtccgg gagggcattc atgaccccca
      721 gcagtcgttc cgagccgaag aggcgcatca ggtcgtcctc cagcgagagg tagaaccggg
      781 agctgcccgg gtcgccctgg cggccggaac ggccgcgcag ctggttgtcg atgcgccgcg
      841 cctcgtggcg ttcggtgccc aggatgtgga ggccgcccag ttcgaccacc tccagcccct
      901 ccaggctttt gccctcgagc tgtcccggcg agacgccggg aaaggccgcc tgaatcgcct
      961 cgaccttgcg ccggtgttcg tcggtccagt cctcgcggcc ggattgcgag ccgcccagga
     1021 tgatgtcggt gccccggccg gccatgttgg tggcgatggt caccttgtac ttgcggccgg
     1081 cctcggccac gatggccgcc tccttctcgt ggtacttggc gttgagcacc tggtgcggaa
     1141 tgttgcgccg ggtgagcatc cgggagagca gctccgattt ctcgatggag acggtaccga
     1201 ccaggaccgg ctggccgcgc cggtaaagtt cctcgatctc ctggaccgcc gccttgtact
     1261 tctcctgggc cgttttgtag atggcgtccg gatggttgac ccggatcagc ggccggttgg
     1321 gcgggatgga gaccacgtcc aggttgtaga tctgcaggaa ctcgaccgcc tcggtggcgg
     1381 cggtgccggt catgccgccc agccgccggt aaaggcggaa gtagttctgg aaggtgatgg
     1441 tggccagggt ctggttttcg gcctcgatct tgacgccttc cttggcctcc agcgcctggt
     1501 gcaggccgtc ggagtaccgg cgcccctcca tcatccggcc ggtgaactcg tccacgatga
     1561 ccaccttgcc gtccttgacc aggtactccc ggtcccgctc gaagccccga tgtgcccgca
     1621 gggcctggtt gaggtggtgg accagctgga tgtgctgcgc gtcgtagagg ttctcgatgt
     1681 tcaggatttt ttcggcgtgg cggatgccgg cctcggtcag gaacccggag ccgttttttt
     1741 cgtcccactc gtaatcggac ttcgtgtcca gacgtcggaa gaccttgtcg atttcgtagt
     1801 agagtgcggt tgactcttcg gccgggccgg agatgatgag cggggtccgg gcctcgtcga
     1861 tcagaatgct gtccacctcg tcgacgatgg cgtagttgag cggccgctgg acgcggtcct
     1921 cgccccggag cgccatgtta tcgcgcaggt agtcgaaacc gaactcgttg ttggtcccgt
     1981 aggttatgtc ggagttgtag gccgcctgcc gctcctcccg gctgctgtcg tgctggatgg
     2041 tcccgacgct caggccgagc gattcgtaga ccggccccat ccagaagcgg tcgcgtttgg
     2101 ccaggtagtc gttgacggtt accaggtggg ctccctttcc ctcgagggcg gccaggtaga
     2161 ggggtagggt ggcgaccagc gtcttgcctt cgccggtggc catctcggcg atgttgccct
     2221 ggaagagggc gatgccgccc aggatctgga cgtcgaaatg gcgcatgttg agccgccgcc
     2281 gggccgactc ccggaccgtg gcgaaggcct ccggcagcag gtcttccagc gtggctcccc
     2341 gctccagccg ctcgcggaac tcgccggtgc gggcggccag ctgctcgtcc gaaagattga
     2401 gcagttccgg ctcgagccgg ttcacctcgg ccagcctggg cagcaaccgg gtgatgtccc
     2461 gctcgctctt gcacttcatg aagcgggcca gcaggccgct gcggttcttg cgggcttcgt
     2521 cgaggatgtg ttgaaagtca gccatggtta cagaaaagaa tttaaacctc ggaagtatct
     2581 ttgtcaaacc ggccgttccg gaagagccgc cggccggctt cgaaggtcgt ttcggcgacc
     2641 gcctccggcc gcacgccctt gatttcggcc agggctccgg ccagcgccgg caggaaggcc
     2701 ggctcgtttc gccgcccgct cttcccctcc ggcggcagga agggcgcgtc ggtctccagc
     2761 agcagccgtt ccagcggcac ctgccgggcc acctcgcgca gcgcctcgtt gcgccggtaa
     2821 gtcaggttgg ccgtgaagga gatctccagc cccagttcca ggacctgtcc ggccaggcgg
     2881 acgtcaccgc tgaaacagtg ccagacgccg cgttttacgc cggccgtcgc cagcgcggcc
     2941 agggtcggcc cggccgcctc ccggctgtgg acgatcaccg gccggccggc ttcgacggcc
     3001 aggtcaagca tccttcgaaa aaccgccgcc tgcttatcgc gatcaacctg gtaatggtaa
     3061 tcgagcccga tttcgccgat ggccaccacc cgcgggtcgg ccagcgcccg ccggaattcg
     3121 gccaggtccg gctccgcctc ctcggcgtgg ccgggatgga agccgagcgc cgcccggagg
     3181 ccaaactccc cggccacctc gatcgccttc cggttgccgg acgggtcggt cccgacgacc
     3241 aggatttcaa cgaccccgtt atcccgggcc cgccggacaa cctcggaccg gtctgagtcg
     3301 aagtccggaa agttcaggtg gcagtgcgta tcgaagagca tgggtttggg ccgggaagac
     3361 tgaggccggc aggcgtggcc tacaaaaaga aagtgccggg acccagagtt gaactgggga
     3421 cgcagggatt ttcagtccct cgctctaccg cctgagctat cccggcaagg gcgcgcgcac
     3481 tcaaaatccg ggcgcgccgc aggtttatta gattaaccca gccgcgggcg tttcgtcaag
     3541 aaggcgcggc ggttttcggt gagcgccgcg gtgctgcgcg ctggacaaaa ctacgccgct
     3601 cgacaacgag cctcgccgtg ttcccgggcc tggtaagacg gcccgggccc cggctactca
     3661 ttgcctcgcc cggcttgttt tgtagccatc gctacgc
//
LOCUS       DAOQYN010000190         3642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_26445_length_3642_cov_4.884583, whole genome shotgun sequence.
ACCESSION   DAOQYN010000190 DAOQYN010000000
VERSION     DAOQYN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26445_length_3642_cov_4.884583"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..506)
                     /locus_tag="PKN80_07390"
     CDS             complement(123..506)
                     /locus_tag="PKN80_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35868.1"
                     /translation="MEWKQFGSEVEVAGRPVPVRSLRLPDELGNLHRYRVTTCWDPAA
                     GFTAVPAEGRLAREKNGLVGAVVLGRNGGHVKIGRRLGCQPFIFVPFNSLSLRTRHRL
                     TRQIRLLLFSDGNFLFGREAAAEAA"
     gene            617..1168
                     /locus_tag="PKN80_07395"
     CDS             617..1168
                     /locus_tag="PKN80_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNQ35869.1"
                     /translation="MRVLLHACCGPCALAPLDKLRSAGHEVEGLWYNPNIHPYSEYQR
                     RLMTAGHVFGIRSARLHIQPPDFEVFLDRLTAAQREGKRCRSCWRWRLEMAAAVARRE
                     GFAAFSTTLLVSPHQENEAIREIGRAAAEKLGIVFLAEDFRPLYPAARKESRSLGLYH
                     QNYCGCLYSEWERFSAVEPGRKS"
     gene            1206..1520
                     /gene="rplS"
                     /locus_tag="PKN80_07400"
     CDS             1206..1520
                     /gene="rplS"
                     /locus_tag="PKN80_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNQ35870.1"
                     /translation="MNADIIRSIEQGYQRELPRVQPGDEVALHLKVVEGGKERTQVFR
                     GTVIALRGSGNGATMTVRKISYGEGVERIVPLNSPNLKKLEVITADRARRAKLYHLRQ
                     AV"
     gene            1522..1911
                     /locus_tag="PKN80_07405"
     CDS             1522..1911
                     /locus_tag="PKN80_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNQ35871.1"
                     /translation="MAGGRDRQRLGARGEALAADFLRRRGYRILARNYRTRLGELDLV
                     ARHGREIVFIEVRTRQGGGFGTPAESVRRLKQSRWRRAAVVFLQQQDWSDRPCRFDFV
                     GVDLSDELAPDIQLLQNLILPEDDFQG"
     gene            1895..3430
                     /locus_tag="PKN80_07410"
     CDS             1895..3430
                     /locus_tag="PKN80_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNQ35872.1"
                     /translation="MISKVNSLGVFGCQAYAVEIEVDLSSGLPGISIVGLPDQAVKES
                     RDRIKPAIKNSGLPFPNRRITVNLAPAEIKKEGPSFDLPIALGLLAAANVGIAPEALK
                     DYAILGELSLEGEVRRVRGVLPMAIFARDGAVRRLIVPAENAAEAAVIETLEVYPVRS
                     LAEAVGFLKGELRIERFRVSPERIFERSARPEPDFSEVRGQELAKRALEVAVAGGHNV
                     LFIGPPGTGKTMLARRVVSILPPFTLDEAVETSQVYSLVGLLPESGLLAERPFRAPHH
                     TVSDVALIGGGQIPRPGEVSLAHNGVLFLDELPEFDRNTLEVLRQPLEEGVVHISRAR
                     SAVSFPASFMFIAAMNPCPCGNLGHPRKACRCAPAQIQKYRSRLSGPLLDRIDLQIEV
                     PPVTVAELSDDRRIGESSRAVRERVASARRLQRERYRSAGILNNSQLAGRRLKEFGRL
                     EPEAESLLNAAVDKFELSARVYDRLRKVARTIADLEASGPIQSHHISEALQYRTLDRK
                     VWI"
BASE COUNT          610 a         1209 c         1204 g          619 t
ORIGIN      
        1 agttttctgg aagcggtgcg gaaggataag ccggtattga ccaccccggc ggagatactc
       61 ggggtgagcc ggatcatcga agggatctat cgttcagcgg ccgccggcgc cgaagtaaag
      121 ctttaggcgg cttcggccgc ggcctcccgg ccgaagagaa agttgccgtc gctgaagagg
      181 agcagccgga tctggcgggt caaccggtgc cgggtccgca ggctcagcga attgaaggga
      241 acgaagatga acggctggca gccgagccgc cggccgatct tgacgtggcc gccgttgcgg
      301 cccagcacca ccgcgcccac caggccgttc ttctcgcggg ccagccggcc ctcggccggc
      361 acggcggtga agccggcggc cgggtcccag caggtggtga cgcggtaacg gtgcaggttg
      421 cccagttcgt ccgggaggcg gagcgagcgc accggcacgg gccgcccggc cacctcgacc
      481 tcggaaccga attgtttcca ttccatcgct tagattttac cataacccgg gggcgtcgtc
      541 ggcccgaagc tcccgggatg cgcggccgtt tgcaattgac ggcggacgcg ctggaatgct
      601 aaaataagca ccgataatgc gggttttact gcatgcctgt tgcggtccct gcgccctggc
      661 gccgctggat aaacttcggt cggccgggca cgaggtcgag ggcctctggt acaatccgaa
      721 tatccatccc tactccgagt accagcgccg cttgatgacc gccggccatg tttttggcat
      781 ccggtcggcc cggcttcata tccagccgcc cgacttcgag gtttttctgg atcgcctgac
      841 cgccgcccag cgggaaggaa agcgttgccg ttcctgctgg cgctggcgcc tggagatggc
      901 ggccgcggtc gcccggcgcg aaggtttcgc ggccttcagc acgaccctgc tggtaagccc
      961 gcatcaggag aacgaggcga tccgggagat cggccgggcc gcggccgaaa aactgggaat
     1021 cgtctttctg gcggaggact tccggccgct ctacccggcg gcccggaagg agagccggag
     1081 tttgggcctt taccaccaga attactgcgg ttgcctttac agcgagtggg agcggttttc
     1141 agcggtggaa cccggccgga agagttgaat ctccgacgcc cggaaatacc aaaaggaggc
     1201 tcaagatgaa tgccgatatc atccgttcca ttgaacaggg ttaccagcgg gaactgccgc
     1261 gggtccagcc cggcgacgaa gtcgccctgc acctgaaggt ggtcgagggc ggcaaggagc
     1321 gcacccaggt tttccggggg accgtcatcg ccctgcgggg ctccggcaac ggcgccacca
     1381 tgacggtgcg caagatctcc tacggcgagg gagtggagcg gatcgtgccc ctcaacagcc
     1441 cgaacctgaa gaagctggaa gtgatcaccg ccgaccgggc ccgccgggcc aaactgtacc
     1501 acctgcgcca ggccgtctga gatggccggc ggccgggacc gccagcggtt gggcgcccgg
     1561 ggtgaagcgc tggcggccga tttcctgcgc cggcgcggtt accgcatcct ggcccgcaat
     1621 taccggaccc gcctgggcga acttgacctg gtggcccgcc acggccggga gatagtcttt
     1681 attgaagtcc gcacccgcca gggaggtggg ttcggcacgc cggccgagtc ggtgcggcgt
     1741 ctgaagcaat cgcgctggcg ccgggccgcg gtcgtttttc tccagcagca ggattggtcc
     1801 gaccgtcctt gccggtttga tttcgtgggc gttgaccttt ccgacgagct ggccccggac
     1861 atccagctgc ttcaaaacct tatccttccg gaagatgatt tccaaggtta attcgctcgg
     1921 ggttttcggc tgccaggcct acgcggtcga aatcgaggtg gacctttcca gcggtttgcc
     1981 cggcatctcg atcgtcgggc tgcccgacca ggcggtcaag gaaagccgcg accgcatcaa
     2041 gccggccatc aagaactccg gcctgccctt tcccaaccgc cggatcaccg tcaacctggc
     2101 cccggccgag atcaagaagg agggtccctc cttcgacctg cccatcgccc ttggcctgct
     2161 ggccgccgcc aacgtcggta tcgcgcccga ggcgctcaag gattacgcca tcctgggaga
     2221 actttccctg gagggagagg tgcgccgggt gcgcggggtg ctgccgatgg ccatcttcgc
     2281 cagggacggc gccgtccgcc ggctcatcgt tccggctgag aacgccgccg aggccgccgt
     2341 catcgagacc ctggaagttt acccggtgcg cagcctggcc gaggcggtcg gctttctgaa
     2401 gggcgagctc cggatcgagc gtttccgggt ttcgccggaa aggatcttcg agcggtcggc
     2461 gcgtccggaa ccggacttct ccgaggtgcg cggccaggaa ctggccaaac gggccctgga
     2521 agtggcggtg gccggcggcc acaacgttct tttcatcggg ccgcccggca ccggcaagac
     2581 catgctggcc cggcgggtgg tctccatcct gccgcccttc acgttggacg aggcggtcga
     2641 gaccagccag gtttacagcc tggtcggact gctgccggag agcggcctgc tggccgaacg
     2701 tcccttccgc gcgccgcacc acaccgtttc cgacgtcgcc ctgatcggcg gcggccagat
     2761 ccccaggccg ggcgaggtca gcctggccca caacggcgtg ctcttcctgg acgaactgcc
     2821 ggaattcgac cgcaacaccc tcgaagtcct gcgtcagccg ctcgaggagg gcgtggtcca
     2881 catttcgcgc gcccgcagcg ccgtcagttt tccggccagt ttcatgttca tcgccgccat
     2941 gaatccctgc ccctgcggca atctcggcca cccgcgcaag gcctgccgct gcgcgccggc
     3001 ccagatccag aagtaccgga gtcgtctctc cggacccctg ctggaccgga tcgatcttca
     3061 gatcgaggtg ccgccggtca cggtggccga actttccgac gaccgccgga tcggggagag
     3121 ttcccgggcg gtccgggagc gggtggcctc ggcccgccgg cttcagcgcg agcggtaccg
     3181 gtcggccggc atcctcaaca acagccagct ggccggccgc cgcctcaagg agttcggccg
     3241 gctggagccg gaggccgagt ccctgctcaa cgcggcggtg gacaagttcg aattgagcgc
     3301 ccgggtttac gaccgtctgc gcaaggtggc ccggaccatc gccgacctgg aggcctccgg
     3361 gcccatccag tcccaccaca tctccgaagc cctccagtac cgcaccctcg accgcaaggt
     3421 ctggatctga acgggcccgc attatcatgg cggcggtcgg gccggttttc aggaatttcg
     3481 aaccaggaat ctggtaagcc aaacgaggcc gggtccgaag attgaaaaag ccgtcgggca
     3541 gcaagatgaa ccgcccaccc ggaattcttc aaatgcttca taaccgcccc tttcaacggt
     3601 ggtttaaacc ccggcggatc ctgccgggcc tggttctggt tc
//
LOCUS       DAOQYN010000191         3551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_27406_length_3551_cov_2.702517, whole genome shotgun sequence.
ACCESSION   DAOQYN010000191 DAOQYN010000000
VERSION     DAOQYN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27406_length_3551_cov_2.702517"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PKN80_07415"
     CDS             complement(<1..359)
                     /locus_tag="PKN80_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006789278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNQ35873.1"
                     /translation="MKERVAVLMSGGVDSSVAAAILKKSGYRVAGFTLRLWDTPAALE
                     SVRDARAVADRLGIPHRVLDFRTAFQTRVLDPFFAAYLAGATPNPCTVCNPAIKFGFF
                     LEKALRIHELIQKHSAI"
     gene            complement(356..1150)
                     /gene="larE"
                     /locus_tag="PKN80_07420"
     CDS             complement(356..1150)
                     /gene="larE"
                     /locus_tag="PKN80_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNQ35874.1"
                     /translation="MEQLCRRLKETGGVLVAFSGGVDSTFLLKTAREVLGERVLAVTA
                     VSATYPDEERRAARRLARSFGVEQLEVETDEWNDPRFRANSPSRCYHCKKHLFRRLSG
                     LAAERNLVLVDATNYSDRLDYRPGDRAARLYRVRRPLLEAGLTKADIRLFSRRLKLAT
                     ADKPAAACLASRIPYGIGLTRSRLRAVAEGEACLRRLGFKGNLRLRHHGSVARLEIDP
                     EFFQRALRFRRAICRSLGRLGFPHVTLDLEGYRTGSLNRGLERAKK"
     gene            complement(1230..1622)
                     /gene="nifU"
                     /locus_tag="PKN80_07425"
     CDS             complement(1230..1622)
                     /gene="nifU"
                     /locus_tag="PKN80_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HNQ35875.1"
                     /translation="MAETKTPYSEKVMDHFRNPRNVGVIENPSGVGKVGNPVCGDVME
                     LFIKVEAGIITEARFRTFGCGAAIATSSMMTEMVKGKTLEEALEISNKAVAEALGGLP
                     PVKMHCSVLAEEALQAAIEDYRKRSGTV"
     gene            complement(1615..2787)
                     /gene="nifS"
                     /locus_tag="PKN80_07430"
     CDS             complement(1615..2787)
                     /gene="nifS"
                     /locus_tag="PKN80_07430"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNQ35876.1"
                     /translation="MERVYLDYAATTPVHPEVIEAMIPFFREKFGNASSLYSFGQETR
                     GALEEVRQRTADFIGAAPEEIIFTSGGSESNNMALKGIAWTARERGGRILVSAVEHHA
                     VSEPAAFLKKQGFEMVRIPVDGQGVVDLDFIRREIDKKPLLVSVMHANNEIGVVQPVA
                     EIGRLCREREVVFHTDAVQTVGHLPVSVRELNADLLSCSAHKLYGPKGIGFLYVRRGI
                     RLSPLIHGGDQERRRRAGTENLPAIVGLGKALEIAGREMEAEGVRLTGYRDRLIDGLL
                     KKVPDCRLNGDRDRRLPNNVNISFAYVEGESVLLNLDFEGIAVSTGSACTSSSLEASA
                     VLLAIGVPAELAHGSIRITMGKWTTEAELDRILEALPRVVAKLRAMSPLYPGGKNG"
     gene            complement(2846..3289)
                     /locus_tag="PKN80_07435"
     CDS             complement(2846..3289)
                     /locus_tag="PKN80_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNQ35877.1"
                     /translation="MKLSTKGRYALRLMLELAQGQSRQPMLLRDIASRQQISPRYLEQ
                     LVLNLKAAGLVKSVRGARGGFLLARPAEKISLLDIYQASEGSSALVECLEDAGLCSRS
                     RECASREVWLAVRNAMASTLQNWTLSQLLDQQREKNRPAEVMYYI"
BASE COUNT          699 a         1104 c         1060 g          688 t
ORIGIN      
        1 atgattgcgc tgtgcttctg gatcagttca tgaatccgga gcgccttttc cagaaaaaac
       61 ccgaacttga ttgccgggtt gcaaaccgta cagggattgg gggtggcgcc ggcaagatag
      121 gcggcaaaaa aagggtccag gacccgggtc tggaaggctg tcctgaaatc aagaacccgg
      181 tgtggaatac ccagacggtc ggccacggcc cgggcatcac gaaccgactc gagggcggcc
      241 ggggtgtccc aaagccggag agtaaacccg gccacccggt aacccgattt cttcagaatg
      301 gctgcggcca ccgaggaatc caccccgccg ctcatcagca ccgccaccct ttctttcatt
      361 tcttggcgcg ctccagcccg cgattcaggc tgccggtccg gtatccctcc agatccaggg
      421 taacgtgcgg aaatcccagc cgcccgagtg aacggcagat ggcccggcgg aagcgcaggg
      481 cgcgttgaaa aaattcaggg tcgatctcga ggcgggcgac cgaaccgtga tggcgcaggc
      541 gcaggtttcc cttgaagccc aaacggcgca ggcaggcctc accctcggcc acagcccgga
      601 gccgggaccg ggtcagcccg atcccatagg gtatccggct ggccaggcag gcggccgccg
      661 gtttgtcggc cgtcgcaagt ttcaaccgtc ttgaaaaaag acggatatcc gccttggtca
      721 ggccggcttc cagaagcggc cggcgcaccc ggtaaagacg ggcggcccgg tctccgggcc
      781 gatagtcaag gcggtcactg taattggtgg cgtcaaccag caccaggttc cgctcggccg
      841 ccaggccgga gagtctccgg aaaagatgct ttttgcagtg gtagcaccgg ctgggagagt
      901 tggctcgaaa acgcgggtca ttccactcgt cggtctcgac ttccagttgt tcaactccaa
      961 aactccgcgc cagccgccgg gccgcccgac gttcctcatc cggataggtt gcactcaccg
     1021 cggtaaccgc cagcaccctt tcgccaagca cctcccgggc cgtctttaaa aggaaggtac
     1081 tgtcaacgcc gccggaaaag gcgaccagga ctccgcccgt ctccttgagc cggcggcaca
     1141 gctgttccat cttttttgcc ggtgatgaca tggaaacccc gttttcgaag taatggaaac
     1201 ctggcagggc agggggcgcg gccttgttat cagacggttc cggagcgttt ccggtaatcc
     1261 tcgatggccg cctgcagggc ctcctcggcc agcaccgagc agtgcatctt gaccggcggc
     1321 agtccgccca gggcttcggc caccgccttg ttggatatct ccagggcctc ttccagggtc
     1381 ttgcccttga ccatttcagt catcatggag ctggtggcaa tggccgcgcc gcagccgaag
     1441 gtgcggaaac gggcctccgt gatgatcccc gcttccacct tgatgaagag ctccatgaca
     1501 tccccgcaga cgggattgcc caccttgccg acccccgacg gattttcaat gacgccgaca
     1561 ttgcgcggat tgcggaaatg gtccatcacc ttttccgaat agggcgtctt cgtttcagcc
     1621 attcttgcct cccgggtaga gtggggacat cgcccgcagc ttggccacca cccgcggcag
     1681 ggcttccagg atccggtcaa gctcggcttc ggtggtccat ttgcccatgg taatccggat
     1741 ggaaccgtgc gccagttcgg ccgggacccc gatggccagc aggaccgccg aagcctccag
     1801 gctgctgctg gtgcaggccg aaccggtgga gaccgcgata ccttcaaaat cgagattcag
     1861 gagaaccgat tcgccttcga cgtaggcgaa agaaatgttg acattgttcg gaaggcgccg
     1921 atcccggtcc ccgttgaggc ggcaatccgg aacttttttg aggagcccgt cgatcaggcg
     1981 gtcccggtaa ccggtcagcc gcacaccctc ggcttccatt tcccggccgg cgatctcgag
     2041 ggccttgccc aaaccgacga tggccggcag gttctcggta ccggcccggc gcctccgttc
     2101 ctggtcaccc ccgtgaatca ggggtgagag tcttatcccg cgccggacat agagaaaacc
     2161 gatgcccttc ggaccgtaaa gcttgtgggc cgaacaggag agcaggtcgg cattcagctc
     2221 gcgcaccgaa accgggagat ggccgacggt ctggaccgcg tcggtatgaa aaacaacctc
     2281 ccgttcccgg cagaggcgtc cgatctcggc caccggctgg acgactccga tttcattgtt
     2341 ggcatgcatc accgaaacca gcagcggttt cttgtcaatc tcccgccgga tgaaatccag
     2401 gtcgacgaca ccctggccat ccaccgggat ccggaccatc tcgaatccct gcttcttcag
     2461 aaaggcggcc ggttcgctga ccgcgtggtg ttcgacggca ctcaccagta tccggccgcc
     2521 ccgttcccgg gccgtccagg caatgccctt gagcgccatg ttgttgctct cgctgccgcc
     2581 cgaggtgaag atgatctcct cgggggcggc gccgatgaaa tcggccgtgc gctgccggac
     2641 ctcttccagg gcgccccgcg tctcctgtcc gaaggaataa agggaggagg cgttgccgaa
     2701 cttctcccgg aagaacggga tcatggcctc gatcacctcc gggtggaccg gggtggtggc
     2761 cgcgtagtcg agatagactc tttccatggt tggatcctct ttttcagaag ggccgggtgg
     2821 ctccttcaga tatctgcact acggttcaga tgtaatacat tacttccgcc ggccggttct
     2881 tttcgcgctg ctgatcaagc agttggctga gcgtccagtt ctgcagggtc gaggccatgg
     2941 cattcctgac ggccagccag acctcccggc tcgcgcactc ccggctgcgg gagcagaggc
     3001 cggcatcctc gaggcactcc accagggccg aggaaccttc gctggcctgg tagatgtcga
     3061 gcaggctgat tttttcggcc ggccgggcca gcagaaaccc cccgcgggcg ccgcgcacgc
     3121 tttttaccag cccggcggcc ttgaggttga gaaccagctg ttccaggtaa cggggcgaaa
     3181 tctgctggcg gctggcaata tcccgtagca gcatcggctg gcgcgactgc ccctgggcca
     3241 gttccagcat caatcgcagg gcataacgcc ccttggtcga aagtttcatg gaaatatatt
     3301 atcaacccgc taaacttttg tcaacttttt tttaatagcg ctgaaatcca gtgattgaca
     3361 accggtattg agtggcgtat aattaaattt caactatcat tttactgata tttatctctc
     3421 ggccggtttt taagaagagg cctgtcaatc aggggagggg caaaatgaaa aaaggaaagc
     3481 agccggaggt ggaaacgcaa gccctgattg acaggcctct tcttaaaaac cggccgagag
     3541 ataaatatca g
//
LOCUS       DAOQYN010000192         3550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_27412_length_3550_cov_4.041488, whole genome shotgun sequence.
ACCESSION   DAOQYN010000192 DAOQYN010000000
VERSION     DAOQYN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27412_length_3550_cov_4.041488"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PKN80_07440"
     CDS             <1..841
                     /locus_tag="PKN80_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35878.1"
                     /translation="VARWGSSTSIAFWELWNEVDLTGYYDSARVKGWHQAVVPYLREI
                     DPWDHLVTTHYCRQNADPLVWVIPEIESIVGNAYSGTVVDAMKDFFLKRVQFAKPVML
                     NEYGVGGNRTALEDNLHAGIWTSSVLPMFGTALFWEWPFIHNFGLYTHYQALARFWKG
                     EDRRGQHLQVSDASLRLPTGSSLTLGVVGMQSEDRAFLWVYDGQKYNGKRGTPNPERF
                     EGVGVEVRALQAGRYRVEYWDTLKGELRQFSEGTVASDGLLPLVLPVFQGDLAVKVKR
                     VQS"
     gene            838..2910
                     /locus_tag="PKN80_07445"
     CDS             838..2910
                     /locus_tag="PKN80_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35879.1"
                     /translation="MKIKFWFLPLLLLLAGTARADWSPSFRKSLTVEVAERELQKLQP
                     VVSVTFPVRGELKTEAGCLLVTDESGRPVPFRLLYFDRRRNEARVIFRSRSAGRYRIL
                     YDGRTAVDSGLDLVPGDTGQVILEDYLYPDIRTSGIWLWTESPRLSGVLSHTQPEGSG
                     TFHSAWLNPNVHYRAGDYLTQYVYLDPARPPEEIMVEVTVRNRRIAFSWGPDRMQWKE
                     LKKVRLGDLPAAGRWQPLKIDLTECGREGDITTLAFYNQGGRAWWDRTCLFQPEAVVR
                     PGLFEERDRKVSAYFTSRVIGPLLFQNQRFFLVNLDGRSSGGATGWEWRFEEKKSSES
                     EFWFRSEGKSGLPVRLTVTGPAGRKAMASDTWTDTVQFPTAAAQELKFLFRELSHQSL
                     INTGETLYLNFLVTNLTPVPLPLTVTDGRESRSLWVLPGKDNSRIADFTIKTSGQPEV
                     RDYRLLAGDLELDRRSFRVQPLGEGLTDIAAAGPYLSGSRGERLVLEVPEFRLENGTA
                     LDTGREISIGIFGDGPPGLASLLKESLARRGVRAAIHEEPGTDTEGYHLLTDSLRLLH
                     DRPRPGYDLALLFPSLPSLRRRSPVQEWRRSMEIQIWALKGRVRRLALVSPLPAAPFA
                     ALFQPYAGAAAEAAGRHGAGFVDAHLFYTGLDDWPRFFRTAPRVYGNFPDPAGLKLLA
                     DYLAAGLF"
     gene            complement(2907..3506)
                     /locus_tag="PKN80_07450"
     CDS             complement(2907..3506)
                     /locus_tag="PKN80_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNQ35880.1"
                     /translation="MLRAIGEGLGLGLANNLYCLGACGAVFLPCLAASGRPLREPVLE
                     LLAGRLLAYLFFGLAVGNINLYFEGRLNPYFLAPLLILLSLWLIGYALGCFRPSGRLC
                     RAAAGPAGRFLPLGAGFVLGISPCPPFLLGLSRSLAGGRFLDPILFFLGFYLGSSLWL
                     LLLLAGGRLKAGRIFQRIGQAAALAAGIWYLGQGIRMLL"
BASE COUNT          596 a         1164 c         1163 g          627 t
ORIGIN      
        1 cgtggcccgc tggggaagtt cgaccagcat cgccttctgg gaactctgga acgaggtgga
       61 tctcaccggc tattacgatt cggcccgggt caagggttgg caccaggcgg tcgtgcccta
      121 cctgagggag atcgatccct gggaccacct ggtcaccacc cattactgcc gccagaatgc
      181 cgatcccctg gtctgggtca ttccggagat cgagagtatc gtcggcaacg cttacagcgg
      241 gaccgtcgtc gatgccatga aggacttttt cctcaagcgc gtccagtttg ccaagccggt
      301 catgcttaac gaatacgggg tgggcggcaa ccggaccgcc cttgaggaca accttcacgc
      361 cggcatctgg acctcgtcgg tcctgcccat gttcgggacg gccctcttct gggagtggcc
      421 gtttatccac aatttcggcc tctacaccca ttaccaggcc ctggcccgtt tctggaaggg
      481 cgaggatcgc cgcggccagc atctccaggt cagcgacgcc tcgctccggc tgccgaccgg
      541 gagcagcctg actctgggtg tcgttggcat gcagtcggaa gaccgggcct tcctctgggt
      601 ttacgacggg cagaagtaca acggcaagcg cggcactccc aacccggaac gcttcgaggg
      661 cgtgggggtc gaggtccggg ccctccaggc cggccgttac cgggtcgagt actgggacac
      721 cctcaagggc gagctccggc agttctccga gggcacggtc gcttccgacg gcctgctgcc
      781 gctggtgctc ccggttttcc agggagacct ggccgtaaaa gtcaaacggg ttcagtcatg
      841 aaaataaaat tctggtttct gcctcttttg ctgcttctgg ccggaacggc ccgggccgat
      901 tggtcccctt ccttccgcaa aagcctgacc gtcgaggtgg ccgagcgcga acttcagaag
      961 ctccagccgg tcgtttcggt gacctttccg gtccgcggcg aattgaagac cgaggccggc
     1021 tgcctgctgg tcaccgacga gtccggccgg ccggtgccgt tccggcttct ttatttcgat
     1081 cggcggcgaa acgaggcccg ggttatcttc cggagccgga gcgccggccg gtaccggatt
     1141 ctttacgacg gccggacggc ggtcgattcc ggccttgacc tggtccccgg ggatacgggc
     1201 caggtcatcc tggaagatta cctttacccg gacatccgga cctccggcat ctggctctgg
     1261 accgagtcgc cgcgcctttc gggggtgctg agccacaccc agccggaggg gtccggtacc
     1321 ttccattccg cctggttgaa tcccaacgtc cattaccggg ccggtgatta cctgacccag
     1381 tacgtctatc ttgatccggc ccggccgcct gaggagatca tggtcgaagt gaccgtgcgc
     1441 aaccggcgga tcgccttctc ctggggaccg gaccggatgc agtggaagga attgaagaag
     1501 gtgcgcctgg gtgacctgcc ggcggccggc cgctggcagc cgctcaagat cgacctcacg
     1561 gagtgcggcc gggaagggga tatcaccacg ctcgccttct ataaccaggg cggccgggcc
     1621 tggtgggacc gcacctgcct gttccagccg gaggccgtgg tccggccggg cctcttcgag
     1681 gagcgggacc gtaaggtgag cgcttacttc acctcccgcg tgatcgggcc gcttcttttc
     1741 cagaaccagc gcttcttcct ggtcaatctg gacggacgtt cttccggcgg ggccactggt
     1801 tgggagtggc gttttgaaga gaagaaatcg agcgagtccg agttctggtt tcgatcggaa
     1861 gggaaatccg ggttgccggt gcgcctgacg gtcaccggtc cggccggccg caaggcgatg
     1921 gcttccgata cctggaccga tacagtccag tttccgaccg cggcggccca ggagctgaag
     1981 tttctcttcc gggagctgtc gcaccagtcg ctgatcaaca ccggcgagac actctacctg
     2041 aatttcctgg tcaccaacct taccccggta ccgcttccgt tgacggtgac cgacggccgc
     2101 gaaagccgct cgctctgggt tttgcccggc aaggataaca gccggatcgc cgatttcacc
     2161 atcaagacct ccggccagcc ggaggtgcgc gattaccggc tcctggccgg cgacctggag
     2221 cttgaccggc gttccttccg ggtgcagccc ctgggtgagg ggctgacgga catcgccgcc
     2281 gccggtcctt acctgtcggg ttcccggggg gaaaggctgg tgctggaggt gccggaattc
     2341 cgtctggaga acggcaccgc gctggacacc ggccgggaaa tctcgatcgg gatcttcggg
     2401 gatgggccgc ccggcctggc gtcgctcttg aaggagtcgc tggcccgccg cggcgtccgg
     2461 gctgcgatcc atgaggagcc ggggacggat accgagggtt atcacctgct caccgattcc
     2521 ctgcgcctgc tgcacgaccg gccgcgcccc ggctatgacc tggccctgct cttcccctcg
     2581 cttccgtcgc tccgacgtcg gagtccggtc caggagtggc gccgttccat ggagattcag
     2641 atctgggcgc tcaagggacg cgtccgccgc ctggcgctgg tttcacccct gccggcggcc
     2701 cccttcgccg ctctcttcca accttacgcc ggggccgccg ccgaggcggc cggccgccat
     2761 ggcgccggct tcgtggatgc ccacctcttc tacaccggct tggatgactg gccgcgtttc
     2821 ttccggaccg cgccccgggt ctatggcaat tttcccgatc cggccggtct gaaactgctg
     2881 gccgattacc tggccgccgg actcttttaa agcagcatcc ggattccctg tcccaggtac
     2941 cagatcccgg cggccagcgc ggccgcctgt ccgatccgct gaaaaatccg gccggccttt
     3001 aggcggccgc cggccagcag gagcagcagc cagagactgg agcccaggta gaagccgagg
     3061 aagaaaagaa tcggatccag gaagcggccg ccggccagtg aacggctgag gccgagcagg
     3121 aagggcgggc aggggctgat gcccagcacg aaaccggccc caagcggcag gaagcggccg
     3181 gccgggccgg ccgccgctcg gcagagtcgc ccggacggcc tgaagcagcc cagtgcgtag
     3241 ccgatcagcc agagggagag caggatcaac agcggggcca ggaagtacgg attcaggcgc
     3301 ccctcgaaat agaggttgat attgccgacc gccaggccga agaagagata ggccagcagc
     3361 cggccggcca gcagttccag aaccggttcc cggagcggcc ggccggaggc cgccaggcag
     3421 ggaagaaaga ccgccccgca ggcgcccagg cagtaaaggt tattggccag ccccagtccc
     3481 aggccctccc cgatcgcccg gagcatcgga actcattcct ggaaggagaa gacggtgaag
     3541 accagcagtt
//
LOCUS       DAOQYN010000193         3544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_27475_length_3544_cov_5.088851, whole genome shotgun sequence.
ACCESSION   DAOQYN010000193 DAOQYN010000000
VERSION     DAOQYN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27475_length_3544_cov_5.088851"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..2226
                     /locus_tag="PKN80_07455"
     CDS             235..2226
                     /locus_tag="PKN80_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35881.1"
                     /translation="MRHNLKDHQTPEWFRAGVVLASNQEAQIFLARCGAGGVNGRESH
                     YRRFGLEELKRLKDLGVNLVFVSGQKGAGFEAEGEEVRYSRAVTERCHQLGMKVGTYI
                     GNTLLSETLFKERPEARGWAVVNAYGEPYYYQSQQTFRFVACRNNPGYRDFLREATRI
                     SLEEIGVDFLHYDNWFSGPEPMWCRCEHCRRRFREFLVRKYRPGDFLDIYGVTSPEAI
                     EPPNLVRYNLPWSGEPWRSPVIRDPLLRDWTDFQCQVLADNLADLAAYARSVKPGVVV
                     ECNPSGLEGTNTYLFKGVDHQRLLPHVDAFWSEEGSYAAITPEGELVNRVRNYKAARF
                     FDQALFTYISGPGRGNEKINAVESLAFNPLSLVGLDPGTPFGRRVIEFRTRHRDLFLE
                     TENISDAAVLRQFESQAAFPAEARLQLQLAEQTLIQGHVPFDLLFDRYLDLAAAYRCL
                     VLPEVVSMSDGQVERVLRMVRDGLGLVVTGAASTFDQHGRRRSSQPIGELWGETDPER
                     NRQSIALRREAGRGRVAYLPRLELCRPVAPDRDWGYLGYRTFQLPGNWRELAGAVEWA
                     AGGFSVYLDGPETVLAEFLRQPEKGRLLVHLVNYRTDAEAAGLRLRFRPELVQGTGGR
                     VRLLSFDPGERRAEARRRPDGWLEVTVDWLETYAIVVIE"
     gene            2408..>3544
                     /locus_tag="PKN80_07460"
     CDS             2408..>3544
                     /locus_tag="PKN80_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35882.1"
                     /translation="MAKETKVGALVRVWSAAAERQRLENLALARRLIRRSIGHQVLVD
                     YRPGHVWYNYAEYPAEPLSIPDEADERKFARYRELGIEVFKIHEEWNDAFEVYGADKF
                     SPNNRRAFRTLIDRVHAHGMKFLPYISTGYYDKRSRRFRSRWANYLNGKIKSLTGGYY
                     DLAECSPRSPEWRAFLLGAVERLMAEYPLDGLYNDVGYNSLAMYDPPDPQLGQVNAFE
                     ESPQRDGAFEDLTQELYTIVKRHRGIYATHFGRYYRSYNNYFTPDPWPPRFQCYDYTF
                     AGEAVGSLDALLRKVRRQPPFVLYIPDWRYIEKQQPGLDSRAVYAISIPYLQFPVLYD
                     GRPITGKKYWGRKLKYAPVATGELPWPDQIAGYYAQAVEAIGTDV"
BASE COUNT          589 a         1193 c         1156 g          606 t
ORIGIN      
        1 cgtccgcttg gggccggaag gcggccgtcg cggcctggct cagacaggag ccgcgagagg
       61 atgatatgcc ggagcgctgt tgcttttaag cttctttccc gaggccgcgg ctccgcgact
      121 cgcctcgttc cgggccctcg gcttaaaggg aacagacgaa aggcaactac aaggatggcg
      181 ggggtggtga ttaacagccg cgtttccccg ccgttttgat aatgggggag aggcatgagg
      241 cataatctca aggatcacca gacgcccgaa tggttccggg cgggtgtcgt gctggccagc
      301 aaccaggagg cgcagatctt cctggcccgg tgcggcgccg gcggcgtcaa cggccgcgag
      361 agccactacc ggcgtttcgg cctcgaggag ctgaagcggc tcaaggatct gggcgtcaac
      421 ctggtcttcg tctccggcca gaagggggcc ggtttcgagg ccgagggcga ggaggtccgt
      481 tactcacggg cggtgaccga acgttgccat cagctgggga tgaaggtcgg cacctacatc
      541 ggcaacacgc tcctctccga gacgctcttc aaggaacggc ccgaggcccg tggctgggcg
      601 gtggtcaacg cctacggcga gccctactac taccagtcgc agcagacctt ccgcttcgtc
      661 gcctgccgca acaaccccgg ctaccgcgat tttctcaggg aggccacccg catcagcctg
      721 gaggaaatcg gggtcgattt tctccactac gacaactggt tcagcgggcc ggaaccgatg
      781 tggtgccgct gcgagcactg ccgccgccgc ttccgggagt tcctggtccg caagtaccgc
      841 cccggcgact tcctcgatat ctacggagtg accagccccg aggcgatcga accgcccaac
      901 ctggtccgct acaacctgcc ctggtccggc gaaccctggc ggtcgccggt gatccgggat
      961 ccgctcctgc gtgactggac cgacttccag tgccaggttc tggccgataa cctggccgac
     1021 ctggccgcct atgcccgttc ggtcaagccg ggggtggtgg tggaatgcaa cccctccgga
     1081 ctggagggga ccaacaccta cctcttcaag ggcgttgacc accagcgcct gctgccccac
     1141 gtggacgcct tctggagcga ggagggtagt tacgccgcca ttaccccgga gggcgaactg
     1201 gtcaaccggg tgcgcaacta caaggcggcc cgcttcttcg accaggcgct cttcacctac
     1261 atctccggcc ccggccgcgg caacgagaag atcaacgcgg tcgaatcgct ggccttcaat
     1321 cccctctccc tggtcggcct cgacccggga acgcccttcg gccgccgggt catcgaattc
     1381 cggacccgcc accgggacct ctttctggag accgaaaaca tctccgacgc ggccgtcctg
     1441 cgccaattcg aaagccaggc cgcctttccg gccgaggcgc gcctccagct tcagctggcc
     1501 gagcagacgc ttatccaggg ccatgttccc ttcgacctgc tctttgaccg gtacctggac
     1561 ctggccgccg cctaccgctg cctggtcctg cccgaggtgg tgtcgatgtc cgacggccag
     1621 gtggaacggg tgctccggat ggtccgggat ggcctgggcc tggtggtcac cggggcggct
     1681 tcgaccttcg accagcacgg ccggcgccgg tccagccagc cgattggcga actctggggt
     1741 gagacggatc cggagcgcaa ccgccagtcc atcgccttgc gccgagaggc cggccgcggc
     1801 cgggtggcct acctgcctcg cctggagctc tgccggccgg tggcgccgga ccgcgactgg
     1861 ggctacctgg gctaccggac cttccagctg ccgggcaact ggcgggaact ggccggggcg
     1921 gtcgagtggg ccgccggcgg cttctcggtt tacctggacg gtccggagac ggtgctggcc
     1981 gagttcctgc gccagccgga aaaggggcgt ctcctggtcc acctggtcaa ctaccggacc
     2041 gacgccgaag cggccggcct gcgcctgcgc ttccggcccg agctggtcca gggcaccggc
     2101 ggccgggtcc ggctgctctc cttcgacccg ggcgaacgcc gggccgaagc gcgccggcgg
     2161 cccgacggct ggctggaggt gacggttgac tggctggaga cctacgccat cgtggtgatc
     2221 gagtgattgg ccgggtcccg gccggcggtg cgtgccgcca gggcgggcgg caaattccgc
     2281 ccgcggttaa aaagcggatc ctcttttgag gccgccgcgg ctgtgatccg gccgtcttct
     2341 tttacgaggt gcccctacga caaccgcggc gccggcggcc ttgaaaaggt ttcgggagaa
     2401 ttcggagatg gcgaaagaaa cgaaggtcgg ggccctggtg cgggtttggt cggcggcggc
     2461 cgaacggcag cgcctggaga acctggccct ggcgcggcgg ctgatccgcc gttccatcgg
     2521 ccaccaggtc ctggttgact accggccggg ccacgtctgg tataactatg ccgagtatcc
     2581 ggccgaaccg ctttcaatac cggacgaggc cgatgagcgg aagttcgccc gctaccggga
     2641 actgggaatc gaggtcttca agatccacga ggagtggaac gacgccttcg aggtttacgg
     2701 cgccgacaag ttctcgccca acaaccggcg ggccttcagg accctgatcg accgggtcca
     2761 cgcccacggg atgaagttcc tgccctacat ctccaccggc tactacgaca agcgcagccg
     2821 ccgctttcga tcccggtggg ccaattacct gaacggaaag atcaagtcgc tgaccggcgg
     2881 ttactacgac ctggccgagt gttcgccccg gagcccggag tggcgggcct tcctgctggg
     2941 ggccgtcgag cggctgatgg ccgagtaccc gctggacgga ctttacaacg acgtgggcta
     3001 caacagcctg gccatgtacg atccgcccga tccgcaactg ggccaggtga acgccttcga
     3061 ggagtcgccc cagcgggacg gcgcctttga agacctgacc caggaactct acacgatcgt
     3121 caagcgtcac cggggcatct acgccaccca tttcggccgc tactatcgtt cctacaacaa
     3181 ctacttcacg cccgatccct ggccgccccg gttccagtgt tacgactaca ccttcgcagg
     3241 cgaagcggtc ggcagcctcg acgccctcct gcgcaaggtt aggcgtcagc cgcccttcgt
     3301 actctacatt cccgactggc gctacatcga aaaacagcag cccggcctgg attcccgggc
     3361 cgtgtacgcc atctcgattc cctacctgca gtttccggtt ctttacgacg gccggccgat
     3421 caccggcaag aagtactggg gtcggaagtt gaaatatgct ccggttgcga ccggcgagct
     3481 tccctggccg gaccagatcg ccggttatta cgcgcaagcg gtggaagcca tcggcacgga
     3541 tgtg
//
LOCUS       DAOQYN010000194         3517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_27765_length_3517_cov_4.491046, whole genome shotgun sequence.
ACCESSION   DAOQYN010000194 DAOQYN010000000
VERSION     DAOQYN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27765_length_3517_cov_4.491046"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKN80_07465"
     CDS             <1..248
                     /locus_tag="PKN80_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35883.1"
                     /translation="CAAILEGEPLIAPGSEGLWSVEFINALILSGRQGRPVDLPLDRD
                     EYDRLLEDLRRNSRPKQVVRIQRKVDPKFKINQGKAK"
     gene            245..1432
                     /gene="argJ"
                     /locus_tag="PKN80_07470"
     CDS             245..1432
                     /gene="argJ"
                     /locus_tag="PKN80_07470"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNQ35884.1"
                     /translation="MSVTYPRGFRASGMSSGIKENGRPDLALVVSDRPARAAALSTAN
                     RFKAASLLVSLEHLRRGEKRAVLINSGNANCLNGRAGLEMTRRLVAELAAALQVPVEE
                     VLFASTGKIGLLLPESKITGALPSLLAGLGDHGHAAAEAIMTTDRVAKEYRFETGLAG
                     RTGPVRLGGMAKGVGMIRPELATMIAVITTDAAIEASALRQALKESVQESFNMLTVDE
                     DQSTNDFVLVLANGAAGNPRIKAGTPAYAVFRDALRDCCVDLTRQLAADGEGAEKLIV
                     VKVGGAWSLRDARRIARRIAGSSLVKSMISGSNPNWGRVLAAAGSCAARIRPEKVRLD
                     LAGVPVFAAGEKIAGDPAELRNRLEKKEIMIELDLGLGAFSATAYGCDLTEEYVRINQ
                     EYS"
     gene            complement(1436..2254)
                     /locus_tag="PKN80_07475"
     CDS             complement(1436..2254)
                     /locus_tag="PKN80_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ35885.1"
                     /translation="MKKSISRWALPADWPLERCAAIARAAGFEAMELALDENGGDLNL
                     LSGPDQASAVAAAIKKAGLEVASLATMLFWKYPLSSRDEAVRERATAVLGKMIELAPA
                     LKTDTLLVVPGMVDEQTPNEIAWQRSQESIKSLLPAAAKAGVVLGIENVWNKMLLTPL
                     EMKWYIDEFRHPAVQAYFDVGNMLVSGYPQHWIRILGSRVKRVHLKDFKLSVGNIEGF
                     CDLLEGDVDYPEVMRALRDIGYDGYLTVEVFPRRHHPESLVHASGRALDFILGT"
     gene            2381..2944
                     /locus_tag="PKN80_07480"
     CDS             2381..2944
                     /locus_tag="PKN80_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNQ35886.1"
                     /translation="MKTTSVLFAGVGGQGIVLASRILAQAAFESGLMVKETELHGMAQ
                     RGGSVISQVRFGFEVHSPLILRGEASRLVATEELEGLRYLDYLRPGARVILNRRRIAP
                     AGGGDGYPSDTAEALEKRGFKVTAVPAPEAAREIGNPRIENTVLLGALSRHLDLPVAA
                     WEKAIAALVPSRTLEVNLRAFRAGRRF"
     gene            2983..>3517
                     /locus_tag="PKN80_07485"
     CDS             2983..>3517
                     /locus_tag="PKN80_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNQ35887.1"
                     /translation="MFWQKEIETLSRDKLSALQLERLGRTLARVYERVPFYRKAMDGN
                     GFRPEDLRGLKDITALPFTTREDLRAGYPDGLLAVERTEAVRLHTSSGTTGKPKAIFF
                     SRRDLDQAAELLARCLVMTGTGPGDVLQNMMTYGLFTGALMMHYAAEKVGILVIPSGP
                     GNTERQLDLMRDFGTTVL"
BASE COUNT          615 a         1148 c         1120 g          634 t
ORIGIN      
        1 tctgcgccgc gattctggag ggggagccgc tgatcgcgcc gggttccgag ggtctctggt
       61 cggtggaatt catcaacgcc ctgatcctgt ccggccgcca ggggcggccg gtggacctgc
      121 ccctggaccg ggatgagtac gaccggcttc ttgaagatct gcgccgtaat tcccggccga
      181 agcaggtggt ccggatccag cgtaaagttg atcctaagtt caaaatcaac caggggaaag
      241 ctaaatgagt gtaacctatc cgaggggatt ccgggccagc gggatgtcgt ccggaatcaa
      301 ggagaatggc cggcccgacc tggctctggt ggtctccgac cgtccggccc gggccgccgc
      361 cctatccacc gccaaccgtt tcaaggccgc ctcgcttctg gtcagcctcg aacatctgcg
      421 tcgcggcgaa aagcgggccg tcctgatcaa cagcggcaat gccaactgcc tgaacgggcg
      481 ggccggcctg gagatgaccc ggcgcctggt ggccgagctg gccgcggcgc tgcaggttcc
      541 ggttgaagag gtccttttcg cctccaccgg caaaatcggc ctgctgctgc ccgaatcgaa
      601 gatcaccggc gccctgccct cgctgctggc cgggctgggc gaccacggtc atgccgccgc
      661 cgaggccatc atgaccaccg accgggtggc caaggagtac cggttcgaga ccggactggc
      721 cggccggacc ggtccggtgc ggctgggagg catggccaag ggtgtcggca tgatccgccc
      781 ggaactggcc accatgatcg cggtcataac caccgacgcc gccatcgagg cctcggctct
      841 gcgccaggcc ctgaaggagt cggtccagga gagttttaac atgctcaccg tggacgaaga
      901 ccagtccacc aacgatttcg tgctggtgct ggccaacggg gcggccggga atccgcggat
      961 caaggccggc acccctgcct atgccgtctt ccgggatgcc ctgcgcgact gctgcgtcga
     1021 cctgacccgc cagctggcgg ccgacggcga aggggcggaa aagctgatcg tggtgaaggt
     1081 gggcggcgcc tggtcactcc gggacgcccg gcggattgcc cgacgcatcg ccggcagcag
     1141 tctggtcaaa agcatgattt ccggaagcaa cccaaactgg ggacgggtgc tggccgcggc
     1201 cggcagttgt gccgcccgca tccgaccgga aaaggtgcgg ctggatctgg ccggggtgcc
     1261 ggtattcgcg gccggggaaa agatcgccgg cgacccggcc gagctccgga accgtctgga
     1321 gaaaaaggaa attatgattg agctggacct cgggctcggt gccttctcgg ccaccgctta
     1381 cggctgcgac ctgaccgaag aatacgttcg gatcaaccag gaatattcct gaagctcagg
     1441 ttccgaggat gaagtcgagc gcccggcctg aggcgtgtac cagcgattcg ggatggtggc
     1501 gccgcggaaa gacctccacg gtcaggtagc cgtcgtaacc gatatcccgc agggcacgca
     1561 tcacctccgg atagtccacg tcgccttcca gcaggtcgca gaaaccctcg atgttgccta
     1621 ccgaaagttt aaaatccttc aggtgaacac gcttgacacg gctcccgaga atccggatcc
     1681 agtgctgggg atacccgctg accagcatgt tgccgacatc gaaataagcc tggacggccg
     1741 ggtggcggaa ctcgtcgatg taccatttca tttccagcgg agtgagcagc atcttgttcc
     1801 agacgttttc gatgcccagt accactccgg ctttggccgc cgccggcagc aggcttttga
     1861 ttgactcctg actgcgctgc caggcaatct cgtttggcgt ctgttcgtcc accattcccg
     1921 gcaccaccag cagggtgtcg gtcttgagcg ccggcgccag ctcgatcatt ttgcccagaa
     1981 ccgccgtcgc ccgttcccgg accgcctcgt ccctggaact gagcgggtac ttccagaaaa
     2041 gcatcgtggc caggctggcg acttccagcc cggccttctt aatggcggcg gccaccgcgg
     2101 aggcctggtc cggcccggaa agcaggttca ggtcgccgcc gttttcgtcc agcgccaatt
     2161 ccatcgcttc aaaaccggct gcgcgggcga tcgccgcgca gcgttccagt ggccagtcgg
     2221 ccggcaaggc ccagcggctg atgcttttct tcattccgtt cctccctttt aatgggtgtt
     2281 tcggatatta taacattacc aggccgtccc ggcggtcgcg tcttccccgg ggcgcatttt
     2341 ccattgtcag gttcccgatt ctgtgttaaa atttttggca atgaagacaa ccagcgtcct
     2401 cttcgccggc gtgggcggcc agggcatcgt cctggccagc cggatcctgg cccaggccgc
     2461 cttcgagtcc ggcctgatgg tcaaggagac cgagctgcac ggcatggccc agcgcggcgg
     2521 cagcgtgatc agccaggtgc gtttcggttt tgaagtgcac tcgcccctga ttcttcgggg
     2581 cgaggccagc cgcctggtgg ctaccgagga gctggaggga ctccgttatc ttgattacct
     2641 gcggccggga gcccgggtga tcctcaaccg gcggcgaatc gccccggccg gcggcgggga
     2701 cgggtatcct tccgataccg cggaggccct ggaaaagagg ggtttcaagg tgaccgcggt
     2761 cccggcgccg gaggcggccc gggaaatcgg caacccccgg atcgagaaca ccgtcctgct
     2821 gggcgccctc tcccgccacc tcgacctgcc ggtcgccgcc tgggagaagg cgatcgccgc
     2881 cctggtgccg tcccggaccc tggaggtcaa cctccgggcc ttccgggccg gacgccggtt
     2941 ttgacgccga ctcgcggtct ttcccccggc caaggagcaa acatgttctg gcagaaagaa
     3001 atcgaaaccc tttcccggga taaattgagc gcgctccagc tggaacggct gggccgcacc
     3061 ctggcgcggg tttacgaacg ggttcccttt taccgcaagg ccatggacgg taacggtttc
     3121 cgaccggaag atcttcgcgg gctcaaagac ataaccgctt tgccgttcac cacccgggaa
     3181 gacctgcggg ccggctaccc cgacggcctg ctggccgtgg agcgtaccga ggcggttcgc
     3241 ctgcacactt cttccggcac caccggaaaa ccaaaggcca tcttcttctc caggcgcgat
     3301 cttgaccagg ccgccgaact gctggcccgt tgcctggtga tgaccggcac cggaccgggg
     3361 gatgtcctcc agaacatgat gacctacggc cttttcaccg gggccctgat gatgcactac
     3421 gccgccgaga aggtcggcat cctggtcatt ccatccggtc ccggaaacac cgaacgccag
     3481 ctcgacctga tgcgggattt tggaaccacg gtccttc
//
LOCUS       DAOQYN010000195         3515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_27794_length_3515_cov_4.367052, whole genome shotgun sequence.
ACCESSION   DAOQYN010000195 DAOQYN010000000
VERSION     DAOQYN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27794_length_3515_cov_4.367052"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKN80_07490"
     CDS             complement(<1..592)
                     /locus_tag="PKN80_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ35888.1"
                     /translation="MKNNGLRQMRLARGLSLAGLAEELGGLVTRQALHNYERGLSRPS
                     PEVARRLTRVFGLDPSLLNGRRERPIHCVPAGYRHYAGITQKEKKRIEAAMLQALEAR
                     LRVQYLLYPAAGGRMPPAISVRDAVQAEAAAAEIRRRWDLGRGPIASMTGLLEEKFIH
                     VIEMETDDRFDGVAVTIRDAKEKSVGAAVVNRVGVCG"
     gene            882..1265
                     /locus_tag="PKN80_07495"
     CDS             882..1265
                     /locus_tag="PKN80_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ35889.1"
                     /translation="MKKYTIYQLSEKSGYSLRTIRYYVQEGLIEPPAGRGRGGFYFDS
                     HLDKLKEIRALQEKGTRIAAIKSLAAAEKAVPMEEAIPRNVRVGYLIAPGVELQVDRE
                     REETDGAAIRRIVRAARLMLNKKEE"
     gene            1265..>3515
                     /locus_tag="PKN80_07500"
     CDS             1265..>3515
                     /locus_tag="PKN80_07500"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNQ35890.1"
                     /translation="MRELLECGLFSTGQKPIPLVGVKATGTITGRTARIRVCQRFVNR
                     EKKAIEAVYKFPLPEAAAVCGFTIRVGEKTIVGSVEARDEAFEKYDRALAEGDGGFLL
                     DQERPNIFTLSVGNVKPETEVVVETEYVETLDADGREIRFVLPTTISPRYLPADQPDE
                     AGIPVNDLVNPRYALDVPYGISISLEIGNRGGLEAIESPSHPISVGMEGESAKVAFGV
                     ESVKLDRDFVLVMRRQAGFSNRAYRCGDFILVDFTLAEAEDAPGGPASGSEIIFILDC
                     SGSMSGSSIAEAKKALEICLKSMDAGSYFNIYRFGSSFAHLFDRPIEYGDQSLDQALK
                     YVSNTEADLGGTEMYAPLEHACRGAAGEGRKRFVILITDGQVGNEERLAELVGKNAER
                     IKFSAIGIGYGPNEFLLKRLALAGGGLAEFVHPGERIEPKMLNLFGKTFGDYLEAAVV
                     ETGRMESRQAPAEMVFFPGRTGAIFVKVAGNPDRLERITLRGRVNGAAQEWTLPVETV
                     KAEGNAIPVLWARERIRDLEEGGDQTGSRQEGRKKKTVQPEVVKLSKEYGLLSSFASY
                     VGIEKRAAGEKETGAMELRKVPVMLTAGWHGMDKVSFILAADSHKMYGKKKACCFPCR
                     PPAKRAAKKTARRFMPSNAAVPLMRKAPGGDPLIIKILALQRKAGGLDFDDEVAWELG
                     IGREELLAAAGGGPETENETFTIIWTAVVMELLNRRFFQDRDTWFAALAKSRKWLKAA
                     AGKAPLIAGG"
BASE COUNT          691 a         1051 c         1200 g          573 t
ORIGIN      
        1 ccccgcagac gccgacccgg ttgacgaccg ccgccccgac gcttttttcc tttgcgtcgc
       61 ggatcgtgac cgccaccccg tcaaaacgat cgtcggtctc catctcgatc acgtggatga
      121 acttttcctc gagcagcccg gtcatgctgg cgatggggcc gcgccccagg tcccaccggc
      181 gccggatttc ggccgcggcc gcctcggcct ggacggcgtc ccggacggag atggccggcg
      241 gcatccggcc gccggcggcc gggtaaagaa gatactggac gcggaggcgc gcctcgaggg
      301 cctgcagcat ggccgcctcg atccgcttct tctccttttg ggtgattccc gcgtaatggc
      361 ggtacccggc cggcacgcag tggatcggcc gttcccggcg gccgttcaaa agagaggggt
      421 ccaggccgaa gacgcgggtc aggcgccgcg cgacctccgg ggagggccgg gagagccccc
      481 gctcatagtt gtggagggcc tggcgcgtga ccaggccgcc cagctcctcg gccaggccgg
      541 ccagggagag gccgcgcgcg aggcgcattt gccgcagtcc gttgttcttc atggcttcat
      601 atcctctgtt tacaattata ctattttctt taaaatttgt caactaaaga gaccggggcg
      661 gatgggagcc gggccccggc gtttcctgaa ccgtccctgg ccgggttgct ccgaaaacgc
      721 ccccggcggc tcctcccggc gccggcggtt ttttatgaca gtaaaatcgc ggccgaaggg
      781 gaaaaaaggc cgagggagca gaacgcataa atcctttatt tatcaagtat taaaccccac
      841 ttgacaaaac gacagttaag tgttaatata gtggcatgaa aatgaagaaa tacacgatct
      901 accagctctc cgaaaaaagc gggtattccc tgagaaccat aaggtattac gtccaggagg
      961 ggctgatcga gccgccggcg ggccgggggc ggggcggttt ctatttcgac agccacctgg
     1021 ataaactgaa ggagatccgc gccctgcagg aaaaagggac gcgcatcgcg gccatcaagt
     1081 cgctggcggc cgcggagaag gccgtgccga tggaggaggc cattccccgg aacgtccggg
     1141 tcggttatct catcgcgccc ggcgttgagc tccaggtgga ccgggaacgg gaagaaacgg
     1201 acggcgccgc catccgccgg atcgtccggg cggccaggct gatgttaaac aaaaaggagg
     1261 agtgatgcgc gaattacttg agtgcgggct tttttcgacc ggacagaagc cgatcccgct
     1321 ggtcggggta aaggcgaccg gcaccattac cgggcgcacc gcccgcatcc gcgtctgcca
     1381 gcggttcgtc aaccgcgaga agaaggccat cgaggcggtt tacaagttcc cgctgccgga
     1441 ggcggcggcc gtctgcggct tcaccatccg ggtcggcgaa aaaacgatcg tcggctcggt
     1501 cgaggcgcgc gacgaggcct tcgaaaagta tgaccgggcc ctcgccgaag gagacggcgg
     1561 cttcctgctg gaccaggaac ggccgaacat cttcaccctc tcggtcggga acgtgaaacc
     1621 ggagaccgag gtggtggtgg agaccgagta cgtcgagacc ctggacgcgg acggccgcga
     1681 aatccgtttc gtgctgccga ccaccatctc gccgcgctat ctcccggccg accagccgga
     1741 tgaagcgggc atcccggtga acgacctggt caacccccgc tacgcgctcg acgtcccgta
     1801 cggaatctcg ataagcctcg agatcggtaa ccgcggcggc ctggaagcga tcgaatcgcc
     1861 gagccacccg atcagcgtcg ggatggaggg cgaatcggca aaggtggcct tcggcgtcga
     1921 gtcggtcaag ctggaccgcg atttcgtgct ggtgatgcgg aggcaggcgg ggttctccaa
     1981 ccgggcctac cgttgcggcg attttatcct ggttgatttc acgctggcgg aggcggaaga
     2041 tgccccgggc ggcccggcga gcgggagcga gatcatcttc attctcgact gctccggctc
     2101 gatgagcggc agttcgatcg ccgaggcgaa gaaggcgctc gagatctgcc tgaaatccat
     2161 ggacgccggg tcttacttca acatataccg gttcggctcc tcctttgcgc acctcttcga
     2221 caggccgatc gaatacggcg accaaagcct cgaccaggcg ctgaaatacg tttccaacac
     2281 ggaggccgat ctgggcggca ccgagatgta cgccccgctg gaacacgcct gccggggagc
     2341 ggccggggaa gggcgcaagc gcttcgtcat tctcatcacc gacggccagg tcggaaacga
     2401 ggagcggttg gcggagctgg tcgggaagaa cgcggaaagg attaagttct ccgccatcgg
     2461 catcgggtac gggccgaacg agttcctgct gaaacggctg gccctggccg ggggcggcct
     2521 ggccgaattc gtccatccgg gggagcggat cgagccgaag atgctcaatc tcttcggcaa
     2581 gacattcggg gattatctcg aggccgccgt cgtcgagacc ggccggatgg aaagccgcca
     2641 ggcgccggcg gaaatggtct tcttcccggg gcggaccggc gcgattttcg taaaggtcgc
     2701 tggcaacccg gaccggctgg aacggataac cctcagggga cgagtgaacg gcgccgcgca
     2761 ggaatggacg ctcccggtcg agacggtcaa ggcggagggc aacgccatcc cggtcctctg
     2821 ggcgcgggag cgcatccggg acctggagga aggcggcgac cagacagggt cccgccagga
     2881 agggcggaaa aagaagacgg tgcagccgga ggtcgtcaag ctgtcgaagg agtacggcct
     2941 gctctccagc ttcgccagtt acgtcgggat cgaaaaacgg gcggccgggg agaaggagac
     3001 cggcgcgatg gaactccgga aggtcccggt catgctgacg gccggctggc acggaatgga
     3061 taaggtctcg ttcatactgg cggccgactc gcacaaaatg tatggcaaga aaaaagcctg
     3121 ttgttttccg tgccgtccgc cagctaaacg agcagctaag aaaaccgctc ggcggtttat
     3181 gccgtctaac gccgccgttc cactcatgag gaaagcgccg ggcggcgatc cgctgatcat
     3241 caagatactc gccctgcagc ggaaggcggg cggcctcgat ttcgacgatg aggtggcctg
     3301 ggagctcggg ataggcaggg aggagctcct ggcggcggcc ggaggcgggc cggagacgga
     3361 gaacgagaca ttcaccatca tctggacggc ggtggtcatg gaactgctga accgccggtt
     3421 tttccaggat agggatacct ggttcgccgc gctggcgaag agccggaagt ggctcaaggc
     3481 ggcggccggg aaggcgccgc tgatcgccgg gggca
//
LOCUS       DAOQYN010000196         3511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_27834_length_3511_cov_5.688079, whole genome shotgun sequence.
ACCESSION   DAOQYN010000196 DAOQYN010000000
VERSION     DAOQYN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27834_length_3511_cov_5.688079"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PKN80_07505"
     CDS             <1..916
                     /locus_tag="PKN80_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35891.1"
                     /translation="AEPLPPADPYIQSIGEPEHIHPGFGGVTFARLHRNRPTLDLVLR
                     HESGGGSRRFFGYDNSLKPLWDTFIPRPPGSHIIQTVDIYGRGEDEVCTGMHILDSRG
                     RLVWSAPDAETYPPHGGDHMDQVRMARLQPDRPERIFIALATGHEGLLVYDREKGRRL
                     FRKQFGHVQSVIEGRFVPGRKDLQLWASTRWDSYGIHCVITPAGQVLSRIQPNFSSES
                     IAPVDWSGNGIKLAMVGGRTALPETLGLIDWEGYRVAPIDPAAVGMDAFHGHGSTMLA
                     DLTGDLRPELIVRSQDRLWVYTQAPAAR"
     gene            962..1993
                     /locus_tag="PKN80_07510"
     CDS             962..1993
                     /locus_tag="PKN80_07510"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNQ35892.1"
                     /translation="MREIELHGSAEEMGRQHGELLGDQVREFHRLTQPRKAAFADQLK
                     RATADLAEYLEKLAPFLNRELAALGRAAGFSFEEALQLSHNEDIPGPDRPWPQSGHNC
                     TVVIVKGPDGTLLANTMDISLTYQPFFTLRRVRPDRGYRFVSIERSGVIGTSRGMNEK
                     GLAIGGASAPYGLTAGPGLGLYVGMRLALQTCATVAEAVELFAGHRPPSKGHCYGLVD
                     RTGAGAVIETNHDRMAVRPLTDRYLVAPNRFHASGMIEFNASRGAKNEENCQLRERLL
                     TAFLEAPGGRFDLNTAMDSLRLHDGKADGICRHAYTRFGIVGQPEAVRFFLGDGFPCQ
                     TDFKDVGRI"
     gene            1995..3194
                     /locus_tag="PKN80_07515"
     CDS             1995..3194
                     /locus_tag="PKN80_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35893.1"
                     /translation="MGIEKNEMREIVRRAADWLAGPATIRDPQAESGLVLRYPLREWR
                     YGIKSSYLTKPRTWGVTGSIWHTGMAIRAFLAARRVTGDNKYLEAARKAGGFLLANQN
                     ICPEDPGLHGSLMAYEDVNDHYQTSTAMEGSLGLLELAGATGEAAWRERFLLAVSWLA
                     THAYAGDGLFHDRFEPNSRRFWTGNFPNLGYGGSQRPLVDNAVMLRAWKETGETRYRE
                     IFRETIERLLRDEDPPGNWITYLPCRASGEGEVHGRQAFWWGLPMFAAYDEFGDKRYL
                     EAGLRAAAWYEKLQLRDGGFYYYTNRYGHGFAFNLCTSVCGASGLLWLEATRRGLKDY
                     RPRLARSVEFIKKAQFSDEAADPNIRGGLFERPSEPDGTEAPGFRLRDIAATFTILAL
                     EELLALD"
     gene            3203..>3511
                     /locus_tag="PKN80_07520"
     CDS             3203..>3511
                     /locus_tag="PKN80_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35894.1"
                     /translation="MEKRKKTGKVTIENRHFRLTLDLKKGTGRLFHRTGGGRPFLMAE
                     VRYLALLLDRRQGKPLNLPVVKVEQDSGGGHHWLKLTCFRPELARVTINFNWNDHAPD
                     L"
BASE COUNT          625 a         1205 c         1145 g          536 t
ORIGIN      
        1 cgccgagccg ctgccgccgg ccgaccccta catccagtcc atcggggaac cggagcacat
       61 ccatcccggc ttcggcgggg tcaccttcgc gcggctgcac cggaaccggc cgaccctgga
      121 cttggttctg cgccacgaaa gcggcggcgg cagccgccgc ttcttcggtt acgacaactc
      181 cctgaagccc ctctgggaca ccttcatacc gcggccgccc ggctcccaca tcatccagac
      241 ggttgacatc tacggccggg gcgaggacga ggtctgcacc ggcatgcaca tcctggacag
      301 ccggggccgg ctggtctgga gcgcgccgga cgccgaaacc tacccgccgc acggcggcga
      361 ccacatggac caggtgcgga tggcccgcct ccagccggac cggccggaac gcatcttcat
      421 cgccctggcc accggccacg aagggctgct ggtttacgac cgggagaagg gccggcgcct
      481 cttccggaag cagttcggcc acgtgcagtc ggtcatcgag ggccgcttcg tgcccggccg
      541 caaggacctc cagctctggg cctccacccg ctgggacagt tacggcatcc actgcgtcat
      601 tacaccggcc ggccaggtcc tttcccggat ccagcccaac ttcagctcgg aatcgatcgc
      661 cccggtcgac tggagcggaa acggaatcaa gctggccatg gtcggcggcc ggaccgccct
      721 gccggagacc ctgggcctga ttgactggga ggggtaccgg gtggcgccga tcgacccggc
      781 cgcggtcggg atggacgcct tccacggcca cggctcgacc atgctggccg acctgaccgg
      841 cgatctgcgg ccggaactga tcgtccgcag ccaggaccgg ctctgggtct acacccaggc
      901 gccggcggcc cggtaatcaa aaataccgtt agaaacccgg gattgaaacg gaggcacccc
      961 gatgcgcgag atagaactgc acggttcggc cgaggagatg ggccgccagc acggagaact
     1021 gctgggtgac caggtccggg aattccaccg gctgacccag ccccggaagg cggccttcgc
     1081 cgaccagttg aagcgggcca ccgccgacct ggccgagtac ctcgagaagc tggctccctt
     1141 cctcaaccgc gaactggccg ccctcggccg ggcggccggc ttcagtttcg aagaggccct
     1201 ccaactcagc cacaacgagg atatcccggg tccggaccgg ccctggccgc aatccggcca
     1261 caactgcacg gtggtgatcg tcaagggtcc ggacgggacc ctgctggcca acaccatgga
     1321 catcagcctc acctaccagc ccttcttcac gctgcggcgg gtaagaccgg accggggcta
     1381 ccgtttcgtc tcgatcgaac gctccggggt gatcgggacc agccggggca tgaacgagaa
     1441 ggggctggcc atcggcggcg cctcggcgcc ttacgggttg accgcgggac cggggctggg
     1501 cctttatgtc ggcatgcggc tggccctcca gacctgtgcc acggtggccg aggcggtgga
     1561 actcttcgcc ggccaccggc cgccctccaa gggacactgc tacggactgg tcgaccgcac
     1621 cggcgccggc gcggtgatcg aaaccaacca cgaccggatg gcggtccggc cgctgacgga
     1681 ccggtacctg gtggcgccca accggttcca cgcgtccggg atgatcgaat tcaacgccag
     1741 ccggggagcg aagaacgagg agaattgcca gcttcgggaa cgattgctga ccgcctttct
     1801 cgaggcgccc ggcggccgct tcgacctgaa tacggccatg gattccctcc ggcttcacga
     1861 cgggaaggcg gacgggatct gccggcacgc ctatacccgt ttcggcatcg tcggccagcc
     1921 ggaagcggtc cgcttctttc tgggcgacgg atttccctgc cagaccgatt tcaaggatgt
     1981 cgggaggatt tgacatgggt atcgaaaaga acgagatgcg cgagatcgtg aggcgggcgg
     2041 ccgactggct ggccgggccg gccaccatca gggatcccca ggccgaaagc ggcctggttc
     2101 tgcgttaccc gctccgggaa tggcgctacg gcatcaagag cagttacctg accaagcccc
     2161 ggacctgggg cgtcaccggc tcgatctggc ataccggcat ggccatccgg gccttcctgg
     2221 ccgcccggcg ggtcaccggc gacaacaagt acctggaggc ggcccggaag gccggcggct
     2281 tcctgctggc caaccagaac atctgcccgg aagacccggg cctgcacggt tccctgatgg
     2341 cctacgagga tgtcaacgac cattatcaga cctccaccgc catggagggt tcgctgggac
     2401 tgctggaact ggccggggcc accggcgagg cggcctggcg ggagcggttc ctgctggccg
     2461 tcagctggct ggcgacccac gcctatgccg gcgacggtct cttccacgac cgtttcgaac
     2521 cgaactcgcg ccgcttctgg acgggaaact tcccgaacct gggatacggc ggcagccagc
     2581 ggccgctggt cgacaacgcg gtgatgctgc gggcctggaa agagaccggc gaaacccgct
     2641 accgcgaaat attccgggag acgatcgaac gcctgctccg ggacgaggat ccgcccggaa
     2701 actggatcac ctacctgccc tgccgggcgt ccggcgaagg tgaagttcac ggccgccagg
     2761 ccttctggtg gggcctgccg atgttcgccg cctacgacga gttcggtgat aagcgttacc
     2821 tggaggccgg cttgcgcgcc gccgcctggt acgaaaagct ccagctgcgc gacggggggt
     2881 tctattacta caccaaccgg tacggccacg gtttcgcctt caatctctgc acctcggtct
     2941 gcggggcctc cgggctgctc tggctggaag cgacccgccg gggcttgaag gattaccgcc
     3001 cccggctggc ccggagcgtc gagttcatca aaaaggccca gttctcggac gaggcggccg
     3061 atcccaatat ccggggcggc ctcttcgaac ggccgagcga accggacgga accgaggcgc
     3121 ccggtttccg cttgcgtgac atagccgcca ccttcaccat cctggccctg gaagagctgc
     3181 tcgccctgga ctgaattcaa acatggaaaa gaggaaaaag accgggaaag tcaccattga
     3241 aaacaggcac ttccggctga cgctcgactt gaagaagggc accggccggc tctttcaccg
     3301 gaccgggggc gggcgtccat tcctgatggc cgaggtccgt tacctcgccc tgctgctcga
     3361 ccggcgccag ggcaagcccc tgaatctccc ggtggttaag gtggaacagg attccggagg
     3421 cggtcaccac tggttgaaac taacctgctt ccggccagaa ctggcccggg tcacgatcaa
     3481 cttcaactgg aacgaccacg ccccggacct g
//
LOCUS       DAOQYN010000197         3504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_27925_length_3504_cov_4.197159, whole genome shotgun sequence.
ACCESSION   DAOQYN010000197 DAOQYN010000000
VERSION     DAOQYN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27925_length_3504_cov_4.197159"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKN80_07525"
     CDS             complement(<1..442)
                     /locus_tag="PKN80_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ35895.1"
                     /translation="MLRRLLKFRLQSNNNWPALVITGLYALFSGFWILLTDEFLFRFI
                     KSPAILILASVFKGWLFILGTSALIYFLILWYLLIQRESQAALAESEVRFRILSESAF
                     EGVALIEAERILDVNTRFVEIFGYPIEELRNLEFVKLVIPEHRGM"
     gene            complement(496..1254)
                     /locus_tag="PKN80_07530"
     CDS             complement(496..1254)
                     /locus_tag="PKN80_07530"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNQ35896.1"
                     /translation="MKKAGILLGVVGLLLLAASCATTEESVKTTVTTGGPGVKEVAAY
                     QGPKARVGVASFKIKAAKAGGEIGEGMTDALVSALFRSNRFIVLERGETLNEIKEELN
                     LTQSGYTEAGKAAEKGTWEGADILVMGAVTAFEPEAAGTGGGGLVIPLPAKFGGGFRI
                     SKNEAYVAAELRLVDVRTTRVLNTARVEGKASNFKVGGFGGALIGNVVLAGGLEKYRN
                     TPMEKALMVMLDNAVNEIVKSVPEDYYRYAPATK"
     gene            complement(1426..3426)
                     /locus_tag="PKN80_07535"
     CDS             complement(1426..3426)
                     /locus_tag="PKN80_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35897.1"
                     /translation="MIFVCSEKNDLWLTLQNSSAGGHRRCGDLETARREAAAGSAILM
                     LADGYPVPGPAVSRAFLEGAQAKSLRLYLEYPGALPGLEMGEARQAEWERIVVTSDFF
                     APALEKGSLLALHGCWFLPVEPRAAHLAAARVAGYRRAVYGIPAAAFPILLQPEHYPG
                     LLVATSQLSNFITGRYGPQESWRKVWTAILAWLEPGESPPRLNWRMTVRPSFDETDEL
                     PPEAESRARERLTAWFRNQAIYNVAFSRGGFKGVIEGFEAGIDYTGRQRLKTWARADC
                     LAETGMLFACDWSLKHQPDSRRLAGRLMDYLWLSPDFIQADPTSPVYGLCNWYEKGPV
                     FYGDDNARVLIASLCASGLLEETRWDERILRCLLANLRTSGSLGFRRKRFDYPATFQQ
                     VDGWRHLNAEPTLNLQPHFQAYLWAAFLWAYALTGYRGFLDRTANALRLTMEAYPDRW
                     QWTNGLTQEMARMLLPLAWLLRIESRPEYRDWLERILAGLQVDMQPGGAIRERLGALD
                     KGQYPPPASNEKYGTTEAPLIQENGDPACDLLYSLGYAFIGLHEAFCSTADPRLKEAG
                     DRLAAFLCRVQIASESHPYLDGAWMRGFDYRLWEYWGSSADADWGAWSVETGWTNTWI
                     GTTMALRKQGLSLLDTAKADRLREKFPGLRAEMLAESALSGV"
BASE COUNT          696 a         1120 c         1071 g          617 t
ORIGIN      
        1 ccatgccccg gtgttccggg attaccagtt ttacgaattc caggttccgc aactcctcga
       61 tcggataacc gaagatctcg acgaagcgcg tgttgacgtc gaggatccgt tcggcctcaa
      121 tcagggccac gccctcgaag gccgattcgg agaggatccg gaaccggacc tcgctttcag
      181 cgagagccgc ctgcgattca cgctggatga gaaggtacca gaggatgaga aaataaataa
      241 gcgcggaggt tcccaggatg aaaagccaac ccttgaagac gctggccagt attagaatgg
      301 cggggctttt tatgaaccgg aagaggaact cgtccgtcag caggatccag aagccgctga
      361 aaagtgcgta aagaccggta atgaccagag ccggccagtt gttgttgctt tgaagacgaa
      421 atttcagtag tcgcctgagc atgagaacga tggacctacc tcctttctta acaccaatct
      481 ttttggcaaa tcaactcatt tcgtcgccgg cgcgtaccgg taataatcct cgggaacgct
      541 ctttacgatc tcgttcacgg cgttgtccag catcaccatc agggccttct ccatcggcgt
      601 gttgcggtac ttttccagtc cacccgccag gaccacgttg ccgatcaggg ccccgccgaa
      661 accgccgacc ttgaagttgg aggccttgcc ctccacccgc gccgtgttga gaacccgggt
      721 ggtgcggacg tcaaccaggc gcaactcggc ggccacgtag gcctcgttct tcgaaatccg
      781 gaagccgccg ccgaacttgg ccggcaaggg aattaccagg ccgccgccgc cggtcccggc
      841 cgcctcgggt tcgaaggcgg tcaccgcgcc catcaccagg atgtcggccc cttcccaggt
      901 ccccttctcg gccgccttgc cggcctcggt gtaccccgac tgggtcaggt tcaattcctc
      961 cttgatttca ttcaaggtct cgccccgctc cagcacgatg aagcggttgg aacggaagag
     1021 ggcgctgacc agggcgtcgg tcatgccttc gccgatctca cctccggcct tggcggcctt
     1081 gatcttgaag gaggcgaccc cgacgcgggc cttgggcccc tggtaggcgg ccacctcctt
     1141 gacacccgga ccaccggtgg tgaccgtggt cttgacgctc tcctcggtgg tggcgcaaga
     1201 ggccgccagt aaaagcaggc cgactacgcc caaaaggatt cccgccttct tcatatccgc
     1261 ctccggaacc tgaaaaggat gcccctgccg atctcttaaa agattttacc cgactttaac
     1321 gaaaaaggta agccgccggg cccgccatca ttttaccata aacccctgaa aaccgattca
     1381 aacgcttcgg gccgccgctt atgccgaccc gggaaacggc cgccattaaa cgccggagag
     1441 agccgattcc gccagcatct ccgcccgcag gccggggaac ttttcccgga ggcgatccgc
     1501 cttcgcggta tctaagagcg aaaggccttg cttccgcaaa gccatggttg tcccgatcca
     1561 ggtgttcgtc cagccggttt ccaccgacca ggcgccccag tcggcgtcgg cggaactgcc
     1621 ccagtattcc cagagccggt agtcgaaacc gcgcatccag gcgccgtcca agtaagggtg
     1681 tgattcggaa gcgatctgta cccggcaaag aaacgcggcc aggcgatccc cggcctcttt
     1741 cagacggggg tccgcggtgc tgcagaaggc ctcgtgcagg ccgatgaaag cataccccag
     1801 gctgtagaga agatcgcagg ccggatcgcc gttttcctgg atcagcggcg cttcggtggt
     1861 gccgtatttt tcgttggaag ccggcggcgg atactgtccc ttgtcgagag cgcccagcct
     1921 ttcgcggatg gccccgccgg gctgcatgtc gacctgcaat ccagccagga tgcgctcaag
     1981 ccagtcacga tactcgggcc gggactcgat ccggaggagc caggccagcg gcagcagcat
     2041 ccgggccatc tcctgggtca ggccgttggt ccattgccag cggtcgggat aagcctccat
     2101 ggtcagccgg agcgcgttgg ccgtccggtc aaggaaacca cggtagccgg tcagggcgta
     2161 ggcccacaga aaggccgccc acaggtaggc ctggaaatgc ggctgaaggt tcagggttgg
     2221 ctcggcgttc aagtggcgcc agccatcaac ctgctggaag gtggcgggat aatcaaagcg
     2281 ctttctccga aaccccagcg aaccggaggt gcgcaggttg gccaggagac agcgcagaat
     2341 gcgctcatcc cagcgggttt cctccaggag gcccgaggcg caaagcgagg cgatcaggac
     2401 ccgggcgttg tcgtcgccgt aaaaaaccgg ccccttctcg taccagttgc aaaggccgta
     2461 gacgggactg gtcgggtcgg cctggatgaa atccggcgag agccagagat aatccatcag
     2521 gcggccggcc aggcggcggc tgtcgggctg gtgcttcagg ctccagtcgc aggcaaagag
     2581 catgcccgtt tcggcaaggc aatcggcccg ggcccaggtc ttcaaccgct ggcgccccgt
     2641 gtaatctatt ccggcttcaa acccctcgat gacgccttta aaaccacccc gggaaaaggc
     2701 gacgttgtaa atggcctggt tcctgaacca ggcggtcagc cgttcccggg cacgcgattc
     2761 ggcctccggc ggcagctcgt ccgtctcgtc gaaactgggg cgaaccgtca tccgccagtt
     2821 caaccgaggc ggactttcac ctggctccag ccaggccagg atggcggtcc agaccttacg
     2881 ccaggactcc tgcggaccat agcggccggt gatgaaattg ctcagctggc tggtggccac
     2941 cagcaggccg ggataatgtt ccggctggag gaggatggga aaggcggccg ccggaattcc
     3001 ataaaccgcc cggcggtagc cggcgacccg cgccgcggcc aagtgggccg cccgcggctc
     3061 cacgggaaga aaccagcagc cgtgcagggc cagaaggcta cctttttcga gggccggggc
     3121 gaagaaatcc gaggtcacga caatccgttc ccattccgcc tggcgcgctt cgcccatttc
     3181 caggccgggc agggcgccgg gatattccag gtaaagacgc agcgatttgg cctgggcgcc
     3241 ttctagaaaa gcccggctca ccgccggacc cggaacgggg tacccgtcag ccagcatgag
     3301 aatcgccgag ccggccgccg cctcccgccg ggcggtttcg aggtcgccgc acctccggtg
     3361 gccgccggcc gaggaattct gaagggtgag ccagagatcg ttcttttcgg aacagacaaa
     3421 aatcatcggt ttatccccga tctgaaaaat actcccccgc cgccccctga ggtcaggggt
     3481 tcgagccgca tcggcaagcg gtga
//
LOCUS       DAOQYN010000198         3455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_28510_length_3455_cov_4.824412, whole genome shotgun sequence.
ACCESSION   DAOQYN010000198 DAOQYN010000000
VERSION     DAOQYN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28510_length_3455_cov_4.824412"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PKN80_07540"
     CDS             <1..323
                     /locus_tag="PKN80_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35898.1"
                     /translation="SIEVAERDESGRVGRLRIEVSGRSFQVAGRDIQRLRERLGWERL
                     RSTLFDVKLEAGQVVFTGRGWGHGVGLSQWGSKRMAESGFTYQQILQFYFPGTTLGII
                     QSEE"
     gene            384..695
                     /gene="raiA"
                     /locus_tag="PKN80_07545"
     CDS             384..695
                     /gene="raiA"
                     /locus_tag="PKN80_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNQ35899.1"
                     /translation="MELKITIRNQVDFSIFLEEYLREKVEKLERFNNQDVFKVEAIIS
                     EDRFEKVCELLTRVKGKDLIARAQAPDFPVAIDQVVQRLKIQLEKYFGKKIDHKRRSK
                     E"
     gene            692..1153
                     /locus_tag="PKN80_07550"
     CDS             692..1153
                     /locus_tag="PKN80_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNQ35900.1"
                     /translation="MIAEYLVKEAIIPDLRSGDKAGVVREMVEALSKAGSVRDVDGTT
                     EWLLEREKLGSTGIGQNIAVPHAKREDVKKLLVTVGLSARGIDFDALDGDPVNIVFLV
                     LAPSDATGLHLKVLARIARLLKDKVFRNSLRSCKSAQDVFSTIKEEDERAV"
     gene            1150..2925
                     /gene="ptsP"
                     /locus_tag="PKN80_07555"
     CDS             1150..2925
                     /gene="ptsP"
                     /locus_tag="PKN80_07555"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNQ35901.1"
                     /translation="MTKFQGIGVSPGIAIGPVYRLPDLHQKIPDHRLPPEEVEAELQR
                     LDRALEETERELEKIRVQAAKNLGSAAAEIFNVHLLLLKDRGLKARLVRQVKEGWNAA
                     YAVTQVLHDYLDSFQRIDNSFLKDRRGDLEDVGKRLVRNLLGLKIQLSDIREKVILVG
                     YNLSPSETASLSKKDVVAFVTEVGGPTSHTAIMARSLEIPAVVGLKGIDQLFRSGAMM
                     IVDGQAGVVILDPDERLVREYTRMRSNLREASQALESLRGKTATTRDGHRVCLGANIE
                     MPTEDVSVLSHGADEIGLFRTEFLYLNRDDLPDEEEQFRAYRRVVEKLRPMSVTIRTL
                     DLGGDKFSSALGMPEEVNPFLGWRAIRFCLARPDIFRVQLRAILRASAHGKVRMMYPL
                     ISDLEEVKAARAILEEVKEGLRREGRPFDEKMPVGAMIEVPSAALAAGAIARRVDFFS
                     LGTNDLIQYSLAVDRVNERTAHLYNPFHPSVLMLIDHVVKAARAAGIGVGLCGEMAAD
                     PATAFLLIGLGLDHLSMAPLAIPQVKQVIRLTNYREARRFARRILAMESAEQIAGEVR
                     DNHERLLARESKGRPKKKTRSDDQT"
     gene            2912..>3455
                     /locus_tag="PKN80_07560"
     CDS             2912..>3455
                     /locus_tag="PKN80_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ35902.1"
                     /translation="MIRLEGNFSVKSEIASSDGLAGAVRNFDRERMIDRLAGFPGQAG
                     EALGFAESPALRLPARPGSIILSGLGGSAIAGDLLRESFADRLPVPFLINRDYALPAC
                     VNGNTLTFLVSYSGNTEETLAAYRQARERRVSMLALTSGGRLAEMAVADGVPLIRIPA
                     GYPPRTAAGYLYWSLAGLLRR"
BASE COUNT          646 a         1017 c         1154 g          638 t
ORIGIN      
        1 ggagcatcga ggtggcggag cgggacgaaa gcggccgggt cggccggctg cggatcgagg
       61 tctccggccg aagctttcag gtcgccggac gcgacatcca gcggctgagg gagcggctgg
      121 gctgggaacg tctgcgcagc acgctcttcg acgtgaagct tgaagccggc caggtcgtct
      181 tcaccggacg gggctgggga catggggtcg gcctctctca gtggggctcc aagcgcatgg
      241 ccgaatcggg ttttacctat cagcagatac tgcagttcta tttcccggga accacgctgg
      301 gcatcatcca gtcggaagag tagtttccat tttttttacg gcgcggtccg gatgcggccg
      361 ggaacgccga agggaggagt ggagtggagc ttaaaatcac gatacgcaac caggttgatt
      421 tttcaatttt tctcgaggaa tacctgcgcg aaaaggttga aaaactggag cgtttcaaca
      481 accaggacgt tttcaaggtc gaggcgatca tttccgagga ccggttcgag aaggtctgcg
      541 aactattaac ccgggtgaag gggaaagacc tgatcgcccg ggcccaggcc ccggatttcc
      601 cggtggccat cgaccaggtt gttcagcggc tgaagataca gctggaaaag tacttcggta
      661 agaagataga ccataaaagg aggagcaagg aatgattgcc gaatatctgg tgaaagaggc
      721 cattattccc gacctgcgca gcggcgataa ggcgggcgtg gtccgggaaa tggtggaagc
      781 gctgtccaag gcgggatcgg ttcgggatgt ggatggaacg accgaatggc tcctggaacg
      841 ggagaaattg ggcagtaccg gcatcgggca gaacatcgcc gttccgcatg ccaaacggga
      901 ggatgtcaag aagcttctgg tgacggtcgg cctctcggcc cgaggcattg atttcgacgc
      961 cctggacggg gatccggtca acatcgtttt tctggtgctg gctcccagcg acgccaccgg
     1021 cctgcatctc aaggtgctgg cccggatcgc gcggctgctg aaggacaagg ttttccgcaa
     1081 ttccctgcgc agctgcaagt cggcccagga tgtcttcagc accatcaagg aggaagacga
     1141 aagggcggta tgacgaaatt ccagggtatc ggcgtttcac cgggcatcgc cattggtccg
     1201 gtctaccgcc tgcccgatct gcaccagaag attccggacc accggcttcc gcccgaggag
     1261 gtcgaagccg agcttcagag gttggaccgg gcgctggagg agaccgaacg ggaactggag
     1321 aagatccgcg tccaggcggc caagaacctc ggctcggccg cggccgagat cttcaatgtc
     1381 cacctgctcc tgctcaagga ccgcggcctt aaggcccggc tggtgcgcca ggtcaaggag
     1441 ggctggaacg ccgcctatgc ggttacccag gtgctgcacg actatctcga ctccttccag
     1501 agaatcgaca acagctttct caaggaccgc cggggcgacc tggaagacgt gggaaaacgc
     1561 ctggtgcgca acctgctcgg ccttaaaatc cagttgagtg atatccgcga aaaggtgatt
     1621 ctggtcgggt acaacctctc tccctcggaa accgcctcgc tcagcaagaa ggacgtggtc
     1681 gccttcgtca ccgaggtcgg cggtccgacc tcgcataccg ccatcatggc ccgttccctc
     1741 gaaatacccg cggtagtcgg gctgaagggg attgaccagc ttttccggtc gggggcgatg
     1801 atgatcgtcg atggccaggc cggggtggtc attctcgatc cggacgagcg gctggtgcgg
     1861 gagtataccc ggatgcgttc caatctgcgc gaggcctccc aggccctgga gtcgctgcgc
     1921 ggcaaaacgg ccaccacccg ggatggccac cgggtctgcc tgggggccaa catcgagatg
     1981 ccgaccgagg acgtctcggt gctctcgcac ggcgccgatg aaataggcct ttttcgaacc
     2041 gagttcctct accttaaccg ggatgacctg cccgacgagg aggagcagtt ccgggcctac
     2101 cggcgggtgg tcgagaagct gcggccgatg tcggtgacca tccggaccct ggacctgggc
     2161 ggggacaagt tttcctcggc gctgggcatg ccggaagagg tcaacccgtt tctgggctgg
     2221 cgggccatcc gtttctgcct ggcccggccg gacatcttcc gggttcagct gcgggccatc
     2281 ctgcgcgcca gcgcccacgg caaggtgcgg atgatgtatc cgctgatcag cgatctggaa
     2341 gaggtcaagg cggcccgggc cattctggag gaggtcaagg aaggattacg ccgggagggg
     2401 cggccttttg atgaaaagat gccggtggga gcgatgatcg aggttccttc ggccgccctg
     2461 gccgccgggg ccatcgcccg ccgggttgat tttttcagcc tgggcactaa cgacctgatt
     2521 cagtattcgc tggccgtcga ccgggtgaac gagcggacgg cccacctcta caaccctttt
     2581 catccgtcgg tcctgatgct gattgaccac gtggtcaagg cggcccgggc cgccggtatc
     2641 ggggtcgggc tctgcggcga gatggccgcc gacccggcca cggccttcct gctgattggc
     2701 ctggggctcg accacctttc aatggccccg ctggccattc cgcaggtaaa gcaggtgata
     2761 cggctgacaa attaccggga agctcgccgg ttcgcccggc ggattctggc gatggagagc
     2821 gccgagcaga ttgccgggga ggtcagggat aaccacgaac gccttctcgc ccgggagtcg
     2881 aagggacgcc caaagaaaaa gacgaggtcc gatgatcaga cttgagggaa atttttcagt
     2941 taagagcgag atcgcttcgt ccgatggcct ggccggggcg gtccgtaatt ttgaccggga
     3001 gcggatgatc gatcgcctgg ccggctttcc cggccaggcc ggtgaagcgc tgggctttgc
     3061 cgaaagcccg gccctgcggc tgccggcccg gccgggcagc atcatccttt ccggtctcgg
     3121 cggttcggcg atcgccgggg acctgctgcg cgagtcgttc gccgaccggc tgccggttcc
     3181 tttcctgatc aaccgcgatt acgccctgcc ggcttgcgtg aatggaaata ccctgacctt
     3241 tctggtcagt tactccggca acaccgagga gaccctggcc gcctaccgcc aggcccggga
     3301 acggcgagtg tccatgctgg ccctcaccag cggcggccgg ctggctgaaa tggccgttgc
     3361 cgacggcgtg ccgctcatcc ggattccggc cggctacccg ccccggaccg cggctggcta
     3421 cctttactgg tcgctggccg gactgcttcg acggg
//
LOCUS       DAOQYN010000199         3442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_28657_length_3442_cov_4.352524, whole genome shotgun sequence.
ACCESSION   DAOQYN010000199 DAOQYN010000000
VERSION     DAOQYN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28657_length_3442_cov_4.352524"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PKN80_07565"
     CDS             <1..613
                     /locus_tag="PKN80_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNQ35903.1"
                     /translation="MGVNESMYDPAKHSIISNASCTTNCLAPVAKVLIDNFGIVKGLM
                     TTIHAYTNDQVILDFPHKDLRRARAAGLSMIPTTTGAAKAIGLVIPALKGKLDGLAIR
                     VPTPNVSMVDLVLTTEKATSKDEVNAAFKKAIAGPLGRYLDYTEEPLVSKDFNGNPKS
                     SIVDGLSTFVIEGNLVKVMAWYDNEWGYSNRVVDLAQYLIERM"
     gene            648..1841
                     /locus_tag="PKN80_07570"
     CDS             648..1841
                     /locus_tag="PKN80_07570"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNQ35904.1"
                     /translation="MPQAKGVEDLKEIRGRRIFMRVDFNVPMKSGQITDDTRISASLP
                     TIRYLLDQGGRLVLASHLGRPKGKRSAELSLAPAAERLTRLLGRPVGFVGDCVGPEVE
                     AKTSALQDGQALLLENLRFYPEEEANDADFARRLAAGAEIYVNDAFGTSHRAHASTYG
                     VPEILKPAVAGYLLEKEIKNLGRLLENPEKPFLLILGGAKVSDKLGIIKNLLARVDMV
                     ILGGGMAYTFVKAKNWTVGNSLVEEDMVPTAKEILRLTRELHLYDFHTPLDHVIADRV
                     AADAQHLTTERGTIPQNWTGVDIGPQAIEEYEDCIRRARTIFWNGPLGVFELEPFAAG
                     TMAIARAVAASDAFTVIGGGDTIAAVNKAGVADRISHISTGGGASLEFLEGQELPGIA
                     ILDRK"
     gene            1838..2593
                     /gene="tpiA"
                     /locus_tag="PKN80_07575"
     CDS             1838..2593
                     /gene="tpiA"
                     /locus_tag="PKN80_07575"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNQ35905.1"
                     /translation="MRRPIIAGNWKMYKTPSQARELAAGVAAGLKPERAAECEVVLCP
                     PAVDLAAVAEVLRGTPVRLGAQNLYPQDEGAFTGEVSAPMLKELGVSSVIVGHSERRQ
                     YFAETDEFVNLKVKSALKHGLQPILCVGESLAEREAGTTEDRIKTQLAGGLADLTPVD
                     LASLVLAYEPIWAIGTGRTATPEQAQAVHRFIRAWLAGQFGAAAAEAMRIQYGGSVKP
                     DNIRSLMDQPDIDGALVGGASLKADSFLAIVGF"
     gene            2642..>3442
                     /locus_tag="PKN80_07580"
     CDS             2642..>3442
                     /locus_tag="PKN80_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HNQ35906.1"
                     /translation="MRKKCLVLISGGLDSALAARLMQRLGFETEGIAFLTSFQPIPRG
                     GRSHSEEVCGRLGIPLHLVPLDEPFLEIVRSPRYGRGKHLNPCIDCKVLMLRSAWEKA
                     REIGAEFLVTGEVLGQRPMSQNLSALRQIEKASGLKGLIFRPLSARFLDPTRPEQVGL
                     IDRESLPAVQGRGRKLLLALAAELGVSGFSTPAGGCLLTDDGFCRRLSDLMELDLFRG
                     GEVDLVKVGRYLRLGPRLRLIVGRSENENRYLLERAGGRVRLEALGSGP"
BASE COUNT          595 a         1178 c         1109 g          560 t
ORIGIN      
        1 catgggcgtc aacgagtcca tgtacgaccc ggcgaagcat tccatcatct ccaacgcttc
       61 ctgcaccacc aactgcctgg ccccggtggc caaggttctg atcgacaact tcggcatcgt
      121 caaggggttg atgactacca tccacgccta caccaacgac caggtcatcc tggacttccc
      181 gcacaaggac ctgcgccggg cgcgggcggc cggcctttcg atgatcccga ccaccaccgg
      241 cgccgccaaa gccatcggcc tggtcatccc ggccctcaag ggcaagctgg acggcctggc
      301 catccgggtg ccgaccccga acgtttcgat ggtcgacctg gtcctgacca ccgagaaggc
      361 gaccagcaag gacgaggtca acgccgcctt caagaaggcg atcgccggac cgctcggccg
      421 ctacctcgac tataccgagg aaccgttggt ctccaaggat ttcaacggca atccgaagtc
      481 gtccatcgtg gacggactct cgaccttcgt catcgaaggg aacctggtca aggtgatggc
      541 ctggtacgac aacgagtggg gttactcgaa ccgggtcgtc gatctggccc agtacctgat
      601 cgagcggatg taatcgtctc ccgtttgccg aagattgggg gctcaagatg ccgcaggcta
      661 aaggggttga agaccttaag gaaataagag gccggcgtat tttcatgcgc gttgatttca
      721 acgtgccgat gaaatccggg cagatcaccg acgacacccg gatcagcgcc tcgctgccga
      781 ccatcaggta cctgctcgac cagggcggcc ggctggtgct ggcctcgcac ctgggacgtc
      841 ccaagggcaa gcggtcggcg gaactctcgc tggcgccggc ggccgaacgc ctgaccaggc
      901 tgctgggccg cccggtcggt ttcgtgggcg actgcgtcgg accggaggtg gaggccaaaa
      961 cctccgctct ccaggacggc caggcgctgc tgctggagaa tctccgcttc taccctgagg
     1021 aggaggccaa tgacgccgat ttcgcccgcc ggctggccgc cggcgccgag atatacgtca
     1081 acgacgcctt cggtaccagc caccgggccc acgcctcgac ctatggcgtg ccggaaatcc
     1141 tgaaaccggc cgtggccggc tacctgctgg agaaggagat caagaacctg ggccgcctcc
     1201 tcgagaatcc cgagaagccc ttcctgttga tcctgggcgg ggccaaggtc tccgacaagc
     1261 tcggcatcat caagaacctg ctggcccggg tcgacatggt catcctcggc ggcggcatgg
     1321 cctatacctt cgtgaaggcc aagaactgga cggtgggcaa ctcactggtc gaggaggaca
     1381 tggtcccgac ggccaaggag atcctccggc tgacccggga acttcacctc tatgattttc
     1441 acaccccgct tgatcacgtc atcgccgacc gcgtggcggc cgacgcccag cacctgacca
     1501 ccgagcgcgg caccataccg cagaactgga ccggcgtcga catcggcccc caggcgatcg
     1561 aggagtacga ggattgcatc cggcgcgccc ggaccatctt ctggaacggg cctttgggcg
     1621 tcttcgaact ggaacccttc gccgccggca ccatggccat cgcccgggcg gtggccgcct
     1681 ccgacgcctt taccgtcatc ggcggcggcg acaccatcgc cgcggtcaac aaggcgggtg
     1741 tggccgaccg catcagccac atttcgaccg gcggcggcgc ctcgctcgaa tttctcgagg
     1801 ggcaggagct gcccggtatc gccatcctgg accggaaatg agaagaccga tcatcgctgg
     1861 aaactggaag atgtacaaga ctccgtccca ggcccgggag ctggcggccg gcgtggccgc
     1921 cggcctgaag ccggaacggg ccgccgaatg cgaggtggtg ctctgcccgc ccgcggtcga
     1981 cctggccgcg gtggccgagg tgcttcgcgg cacgccggtc cggctgggcg cccagaacct
     2041 gtatcctcag gacgaagggg ccttcaccgg cgaggtttcg gccccgatgc tcaaggagtt
     2101 gggcgtcagt tcggtcattg tcggccactc cgaacggcgc cagtacttcg ccgaaacgga
     2161 cgaattcgtc aacctcaagg tcaagtccgc cctcaagcac ggcctccagc cgatcctctg
     2221 cgtcggcgaa agcctggccg agcgggaagc gggaaccacc gaggaccgga tcaagaccca
     2281 gctggccggc ggcctggccg acctgacccc ggtcgatctc gcctcgctgg tgctcgctta
     2341 cgaaccgatc tgggccatcg gcaccggccg gaccgccacc ccggaacagg cccaggccgt
     2401 ccaccgcttc atccgggcct ggctggccgg ccagtttggg gccgcggccg ccgaagccat
     2461 gcgcatccag tacggcggca gcgtcaaacc cgacaatatc cgctcgctca tggatcagcc
     2521 ggacatcgac ggcgcgctgg tcggcggcgc cagcctcaag gcggactcct tcctggccat
     2581 cgtcggtttt tgacccgggt tcggcgaacc ggatccgccg gccgcgcccc ggaggccccc
     2641 gatgcgaaag aagtgcctgg tgctcatctc cgggggcctg gacagcgccc tggccgcccg
     2701 gctgatgcag cggttgggtt tcgagaccga gggcatcgcc ttcctgacct cttttcaacc
     2761 cataccgcgc ggcggccgga gccactccga agaggtctgc ggccggctgg gtatacccct
     2821 gcacctggtg ccgctggacg aaccgttcct ggaaatcgtc cgctccccgc gttacggacg
     2881 cggcaagcac ctcaacccct gcatcgactg caaggtcctg atgctcaggt ccgcctggga
     2941 gaaggcgcgt gagatcggcg ccgaattcct ggtgaccggc gaagtgctcg gccagcggcc
     3001 gatgtcgcag aacctttcgg ccctccgcca gatcgagaag gcctccggtt taaaggggct
     3061 tatcttccgt ccactctcgg cccgttttct cgacccgacc cggccggagc aggttgggtt
     3121 gatcgaccgc gaaagcctgc cggccgtcca gggccggggg cgcaagctgc tgctggccct
     3181 ggccgccgaa ctgggcgtct ccggattctc gacgccggcc ggcggctgcc tgcttactga
     3241 cgacggtttc tgccggcgcc tctccgactt gatggaactg gacctttttc ggggcgggga
     3301 ggttgacctg gtcaaggtcg gccgctacct gcgcctgggg ccgcgactcc ggctgatcgt
     3361 cgggcggagc gagaatgaga accgctacct gctggagcgg gccggcggcc gggtccggct
     3421 ggaggcgctg ggcagcggtc cg
//
LOCUS       DAOQYN010000200         3402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_29161_length_3402_cov_4.979385, whole genome shotgun sequence.
ACCESSION   DAOQYN010000200 DAOQYN010000000
VERSION     DAOQYN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29161_length_3402_cov_4.979385"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PKN80_07585"
     CDS             <1..562
                     /locus_tag="PKN80_07585"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ35907.1"
                     /translation="RRVGLLQLSEAEIAAVDEGVRTAARRCGPETPFSENPARRLAAV
                     LRGALPVIYSGQYLSGVATRWKTQIEENGKALAFTGLMPEMNHNEVVGWSNPDRLIRE
                     LAVICLREPTDHPQVQRRFEIIPDLLQPRRFLVEVRADSDDRLARLFMLLVLGDWVSY
                     YLALEYGIDPTPVERITALKRQLSKE"
     gene            565..1710
                     /gene="metK"
                     /locus_tag="PKN80_07590"
     CDS             565..1710
                     /gene="metK"
                     /locus_tag="PKN80_07590"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNQ35908.1"
                     /translation="MAEKTDFLFTSESVTEGHPDKVCDQISDTILDSLLAVDPESRVA
                     CEALVTRGLGLVAGEITSRAQIDIPSLVRQVIREVGYTKSEYGFHCCESAVLTAVQKQ
                     SADIALGVDAGGAGDQGLMFGYACDETREKMPLPITLAHALARRLAEVRKKGIVPYLR
                     PDGKSQVTVRYRDGRPCEVICVVLAAQHDPGLKQSRIKADLTEKVIDRALPASLRSRD
                     MKIFVNPTGSFVIGGPQGDTGVTGRKIIVDTYGGFSSHGGGCFSGKDPTKVDRSASYM
                     ARYLAKNIVAARLCRRCEIQLAYVIGKPQPVSIYLNTFGTGRVDEARILRLIRDEFDL
                     SPRAIIKTLDLRKPRYRPTACYGHFGRTEASFTWERTDQAEKFRTLA"
     gene            1736..2323
                     /gene="coaE"
                     /locus_tag="PKN80_07595"
     CDS             1736..2323
                     /gene="coaE"
                     /locus_tag="PKN80_07595"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNQ35909.1"
                     /translation="MLIGLTGVFGSGKSTVLRMLKRRGLATVSADALVHRLLRRQAVK
                     AEIRRRFGPGVFAGDELDRKQLAALIFADRRRRKELERLLHPLVLEIILARAGREAAR
                     GKALAAEVPLLFECGWEKYFDRIICVAARPVTIRARLAAAGWPEAQVRRRLAGQWPLT
                     RKAARSDLVVWNDAGFRKTAAQLEAGLPALLAARK"
     gene            2456..>3402
                     /gene="rho"
                     /locus_tag="PKN80_07600"
     CDS             2456..>3402
                     /gene="rho"
                     /locus_tag="PKN80_07600"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNQ35910.1"
                     /translation="MAENASKQVNILELKAMPMPELQKKAREIGVSGISGLKKQDLIF
                     RFLQAQAERNGLLFGEGVLEVLMPEGFGFLRSTNYNYFPSPDDIYVSPSQIKRFNLIT
                     GDTVSGQIRPPKEGERYFALLRVDAVNGENPEDIRDRVPFEDLTPLYPTKRFILETEK
                     SELSTRVMDLATPLGKGQRGLIVSPPRAGKTVLLQKLAQGITTNNPEVHLIVLLVGER
                     PEEVTEMERSVRGDVISATFDEPPERHVQVAEIVLEKAKRMVEHHKDVVILLDSITRL
                     ARAYNGIVPHTGKIMSGGLESNALEKPKRFFGAARNIEEG"
BASE COUNT          614 a         1066 c         1138 g          584 t
ORIGIN      
        1 tcgacgggtg ggactgcttc aactttccga ggccgagata gccgcggtgg atgaaggggt
       61 gcggacggcc gcccgccggt gcgggccgga gactcccttt tcggaaaacc cggcccggcg
      121 cctggcggcc gtcctgcggg gagccctgcc ggtgatctat agcggccagt acctgagcgg
      181 ggtggccacg cgctggaaga cccagatcga ggaaaacggc aaggcgctgg cctttaccgg
      241 cctgatgccg gagatgaatc acaacgaggt ggtgggctgg tccaacccgg accggctgat
      301 tcgggagctg gccgtgatct gcctgcgcga accgaccgac catccccagg tccagcggcg
      361 cttcgaaatc atccccgacc tcctgcaacc gcgccgattc ctggtcgaag tccgggccga
      421 ttcggacgac cggctggcgc gcctttttat gctgctggtg ttgggcgact gggtgagtta
      481 ctatctggcc ctggaatacg gaatcgatcc gactccggtg gagcggataa ccgcgcttaa
      541 acggcaatta tcgaaggagt agccatggcc gagaaaacgg attttctttt cacctcggag
      601 tcggtgaccg aggggcaccc ggacaaggtc tgcgaccaga tctccgacac catcctggat
      661 tcgctgctgg ccgtcgaccc ggagagccgg gtggcctgcg aggcgctggt gacccggggc
      721 ttggggctgg tggccggcga aatcacctcc cgggcccaga tcgacattcc atcgctggtg
      781 cgccaggtca tcagggaggt cgggtacacc aagtcggaat acggtttcca ctgctgcgaa
      841 agcgcggtgc tcaccgccgt tcagaaacag tcggccgaca tcgcgctcgg ggtggacgcc
      901 ggcggcgcgg gtgaccaggg gctgatgttc ggatacgcct gcgacgagac gcgcgagaag
      961 atgcccctgc cgataaccct ggcccacgcg ctggcccgcc ggctggccga ggtgcggaag
     1021 aagggaatcg tgccttatct cagaccggac ggcaagagcc aggtaacggt tcgctaccgg
     1081 gacggccgtc cctgcgaagt gatctgcgtc gtcctggcgg cccagcacga cccgggcctc
     1141 aaacagtccc ggatcaaggc cgatctgacc gagaaggtta ttgaccgggc gctgcccgct
     1201 tcgctgcgca gccgggacat gaagattttc gtcaatccca ccggcagttt cgtgatcggc
     1261 ggcccgcagg gggacaccgg cgtgaccggc cggaagatca ttgttgatac ctacggcggt
     1321 ttttcgagcc atggcggcgg ttgtttttcc ggcaaggatc cgaccaaggt ggaccggtcg
     1381 gcctcttata tggcccgcta cctggccaag aacatcgtgg ccgcccggct ctgccgccgc
     1441 tgcgaaattc agctggccta cgtcatcgga aaaccccagc cggtctccat ttacctgaac
     1501 accttcggaa ccggccgggt tgacgaggcc cgtatcctgc gcttgatccg ggacgagttc
     1561 gacctgtcgc cccgagcgat catcaagacg ctggacctgc gcaagccgcg ttaccgtccg
     1621 accgcctgct acggacactt cggccgtacc gaggcttcgt tcacctggga gcgtaccgac
     1681 caggccgaga aattccggac gctcgcctga gcggcggcgg acgggccggg aaaccatgtt
     1741 gattggcctg accggggttt tcggatccgg caagagcacg gtgctgcgta tgctcaagcg
     1801 ccgggggctg gcgaccgttt ctgccgacgc cctggtgcac cgcctgctcc ggcgccaggc
     1861 ggtcaaggcg gaaatccggc gccgcttcgg ccccggagtt ttcgcggggg atgaactgga
     1921 tcggaagcag ctggccgcgc tgatctttgc cgaccggcgc cgccgaaaag agctggagcg
     1981 tttgctgcat ccgctggtcc tggaaatcat cctggcccgg gccggtcggg aggcggcgcg
     2041 gggcaaggcg ctggcggccg aggtgccgct cctgttcgag tgcggctggg agaagtattt
     2101 tgaccggatc atttgcgtgg ccgcccgtcc ggttacgatc cgggcgcggc tggcggcggc
     2161 cggttggccc gaagcgcagg tcaggcgccg gctggccggt caatggccct tgacccgcaa
     2221 ggcggcccgg agcgatctgg tggtctggaa cgacgccggg tttagaaaga ccgcggccca
     2281 gctggaggcc ggcttgccgg ccctgctggc cgcccgtaaa tgacgacccc gatggcggcg
     2341 gttttccggc gcgccgccga ccggcgcaaa ctcctttcgc tgttttcaaa atagaaacgc
     2401 gagccggacc ggtccggccc gcatcccgag gttaactcgc agaaagaggt gtccgatggc
     2461 agaaaatgcc agcaagcagg tcaacattct ggaattgaag gctatgccga tgcccgagct
     2521 tcaaaagaaa gcccgggaga taggcgtgag cggtatctcg ggtttgaaga agcaggatct
     2581 catcttccgc tttctgcagg cccaggccga gcgcaacggg cttctattcg gggaaggggt
     2641 gctcgaggtg ctcatgccgg aagggttcgg cttcctgcgc agcaccaact acaactattt
     2701 ccccagcccc gacgatatct atgtttcccc gtcccagatc aagcgtttta accttatcac
     2761 cggcgatacg gtgagcggtc agatccggcc gcccaaggag ggcgagcggt atttcgccct
     2821 gctccgggtg gacgcggtca acggcgagaa cccggaggac atccgggacc gggttccctt
     2881 tgaggacctg acgccgcttt atccgaccaa gcggttcatc ctggagaccg aaaaatcgga
     2941 gttgtccacc cgggtgatgg acctggccac tccgctgggc aagggacagc gcggcctgat
     3001 tgtttcccca ccccgggccg gcaagacggt cctgctccag aaactggccc agggtatcac
     3061 caccaacaac ccggaagtcc atctgatcgt gctgctggtc ggtgaacggc cggaagaggt
     3121 taccgagatg gaacgctcgg tgcgcggcga cgtgatcagc gccaccttcg acgagccgcc
     3181 cgagcgccat gtccaggtgg ccgagatcgt gctggagaag gccaagcgca tggttgagca
     3241 ccacaaggac gtggtgatcc tgcttgacag catcacccgc ctggcgcgcg cctacaacgg
     3301 catcgtgccc cacaccggca agatcatgtc cggcggtctg gagtcgaacg ccctggagaa
     3361 acccaagcgc ttcttcggcg ccgcccggaa catcgaggag gg
//
LOCUS       DAOQYN010000201         3375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_29512_length_3375_cov_3.831024, whole genome shotgun sequence.
ACCESSION   DAOQYN010000201 DAOQYN010000000
VERSION     DAOQYN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29512_length_3375_cov_3.831024"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKN80_07605"
     CDS             <1..295
                     /locus_tag="PKN80_07605"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35911.1"
                     /translation="KLQLGYLRDRLAAREIAIETTAAVEEYLAREGYDPVFGARPIKR
                     LIQREIENPLARELISGNISRRSRVTIDFDGRAIRFRSGAADPKKKGGSDAGQ"
     gene            282..1397
                     /locus_tag="PKN80_07610"
     CDS             282..1397
                     /locus_tag="PKN80_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ35912.1"
                     /translation="MQVNERGLKKLDVEFAGYLEGDRIGVFFKEFEVKFAAGHWCAGG
                     FLDRFATVGYNPGLKSDIISQLKRIAAAGIEGVEFHENIFIDARRRKNPARIAEVKDA
                     LKEYRLVPTNMNTNLWTDPHWKFGGVTNPNRKVREEALEITIQGAEIARELGCTSVAL
                     WPGADGWDYNFEANYGVLLEQFVQACIEINRRAKKLGLRFGIEAKMKEPREGHMIIPT
                     THCAVLVARKVNQACGGQNMGVAVDYGHEMMNGLEPAFNLYVAKEFGVPVVNFHVNSA
                     KWRSNDEDRVAGTANLWQMVDFCYAALDTGYRGWFGEDQFTYRLDPVKAMSLSRELFA
                     NCLKKALLIWRERKALVAAQATGDAGRTLDVVKKMIY"
     gene            1401..3104
                     /locus_tag="PKN80_07615"
     CDS             1401..3104
                     /locus_tag="PKN80_07615"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007276878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HNQ35913.1"
                     /translation="MGNGYSLGLDFGTASVRALLISLDSGAETATAVWNYQHGTDGVI
                     GAADDPHLARQHPADYLDGSAAVLKEVLARAKAADPDFAPERVLGIGVDTTGSTPIPV
                     DGAGRPLCFQDRFRDNPAALAWLWKDHTAVAEAEELTQLAGRLRPDYLRHCGGRYSSE
                     WFWAKILRLARQDPEVYRAAAGFVELADFIPAQLCGAGAPGEIRRSACAAGHKALYSR
                     KWGGLPDEEFLAGLDPRLAGLRARLYDRVFPAGASAGRLSPEWARRTGLPAGCPVSVG
                     AFDAHLGAVGSGIRENGLVKVMGTSTCDLMVFPAAAEARLEAIEGMCGFAEDSIIPGL
                     IGLEAGQSGVGDIFAWFAGHFLPAGYQREAAAAGRDGQQWLTEKAARLKPGSSGLLAL
                     DWNNGNRSILVDQWLTGLLVGQTLGTRPEEVYRSLVEATAFGARVIVDQIEAGGISIG
                     AVIACGGISRKNPFLMQIYSNILERPIRVAGSEQTCALGAAIAGAVAAGAFKDIPAGQ
                     DRCCRFEAADYRPEPAAVPVYRELYRLYRQLHDGFGRPAGPLAGVMKDLLKLKRASNA
                     S"
     gene            3116..>3375
                     /locus_tag="PKN80_07620"
     CDS             3116..>3375
                     /locus_tag="PKN80_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNQ35914.1"
                     /translation="MADLRSAVLEANRLLPEWDLVVSTQGNASGFDPGRGRMIIKPSG
                     VPYDRLKRSDLVTLDLSGRVVAGRGRPSVDWPHHLFLYREVPG"
BASE COUNT          551 a         1088 c         1170 g          566 t
ORIGIN      
        1 gaaactgcag ctgggctacc tgcgcgaccg gctggcggcc cgggagattg ccatcgagac
       61 caccgcggcg gtggaggagt acctggcccg ggagggctac gacccggtct tcggcgcccg
      121 ccccatcaag cgcctgatcc agcgggagat cgagaacccg ctggcgcgcg aactcatctc
      181 cggcaatatc tcccgccgca gccgggtgac gattgatttc gacggccggg ccatccggtt
      241 ccgatccggg gcggcggacc caaagaaaaa gggaggcagc gatgcaggtc aatgaacggg
      301 gcttgaaaaa actggacgtc gagtttgccg gctatctcga gggtgaccgg atcggggtct
      361 tcttcaagga gtttgaggtc aagttcgcgg ccggccactg gtgcgccggc ggtttccttg
      421 accgcttcgc caccgtgggc tacaacccgg gcctgaagtc cgacatcata agccagttga
      481 agcggatcgc ggccgccggc atcgagggcg tcgagtttca cgaaaacatc ttcatcgacg
      541 cccggcggcg caagaatccg gcccggatcg ccgaggtgaa ggacgcgctc aaggagtacc
      601 gcctggtgcc gaccaacatg aacaccaacc tctggaccga tccgcactgg aagttcggcg
      661 gggtgaccaa tccgaaccgg aaggtgcggg aggaggcgct ggaaatcacc atccaggggg
      721 ccgagatcgc ccgggaactg ggctgcacct cggttgctct ctggccgggc gccgacggct
      781 gggactataa tttcgaggcc aattacggtg ttctgctgga acagtttgtc caggcctgca
      841 tcgagatcaa ccgtcgggcc aagaaactcg gcctgcgttt cggtatcgag gccaagatga
      901 aggagccgcg cgaggggcac atgatcattc ccaccaccca ctgcgcggtc ctggtggccc
      961 gcaaggtcaa ccaggcctgc ggcgggcaga acatgggagt ggcggttgat tacggtcacg
     1021 agatgatgaa cggcctggaa ccggccttca atctctacgt ggcgaaggag ttcggggtac
     1081 cggtggtcaa cttccacgtc aattcggcca agtggcgttc caacgacgaa gaccgggtgg
     1141 ccgggaccgc caacctctgg cagatggtcg acttctgcta cgcggccctg gataccggct
     1201 accgcggctg gttcggcgag gaccagttca cctaccggct ggacccggtc aaggccatgt
     1261 ccctgtcacg cgagctgttt gccaactgtc tcaagaaggc gctcctcatc tggcgggaac
     1321 gcaaggcgct ggttgccgcc caggccaccg gcgacgccgg ccggaccctg gacgtggtca
     1381 aaaaaatgat ttattgaagc atgggcaacg gatacagcct cggcctcgat ttcgggaccg
     1441 cctcggttcg ggcgctgctg atttcgcttg attcgggcgc cgagaccgcc acggcggtct
     1501 ggaattatca gcacggaacc gatggcgtga tcggtgcggc cgacgacccg cacctggccc
     1561 gccagcaccc ggccgattac ctggacggaa gcgcggcggt gctcaaggag gttctggccc
     1621 gggcgaaggc ggccgatccg gacttcgcgc cggaacgggt gctcggcatc ggggtcgaca
     1681 ccaccggaag cacgcccatt ccggtggacg gtgccggccg gccgctctgc tttcaggacc
     1741 gcttccggga caatccggcc gccctggcct ggctctggaa ggaccacacc gcggtggccg
     1801 aggccgagga gttgacccaa ctggccggcc gtctccggcc ggattacctg cgccactgcg
     1861 gcggccggta ctcctcggaa tggttctggg ccaagattct ccgcctggcc cggcaggatc
     1921 cggaggtcta ccgggcggcg gccggtttcg tggaactggc cgatttcatc ccggcccagc
     1981 tttgcggcgc cggcgcgccg ggagaaatcc ggcgcagcgc ctgcgccgcc ggccacaagg
     2041 ccctttacag ccggaaatgg ggcggcctgc ccgacgagga atttctggcc gggctggatc
     2101 cgcgcctggc cgggttgcgc gcccgtcttt acgatcgggt ctttccggcc ggcgcttccg
     2161 ccggtcggtt gagtccggaa tgggcccggc gcaccggatt gccggccggc tgcccggtca
     2221 gcgtcggagc ctttgacgcc cacctgggcg cggttggttc gggcatccgg gagaacgggc
     2281 tggtcaaggt gatgggcacc tccacctgcg acctgatggt cttcccggcg gcggccgagg
     2341 cccggcttga ggccatcgag ggcatgtgcg gtttcgccga ggattcgatc atacccggcc
     2401 tgattggcct ggaggccggc cagtccgggg tcggggatat cttcgcctgg ttcgccggcc
     2461 attttctgcc ggccggctac cagcgcgaag cggccgccgc cggccgggac ggccagcagt
     2521 ggctgacgga gaaggcggcc cgcctgaagc cgggatccag cgggctgctg gccctggact
     2581 ggaacaacgg caaccgctcg atcctggtcg accagtggct gaccggtctc ctggtggggc
     2641 agaccctggg gacccgtccc gaggaggtct accggtccct ggtcgaggcc accgccttcg
     2701 gcgcccgggt gatcgtcgac cagatcgaag ccggcggcat ttcgatcggc gcggtgatcg
     2761 cctgcggcgg catctcgcgc aagaaccctt tcctgatgca gatttacagc aatatcctgg
     2821 aacggccgat ccgggtggcc ggttccgaac agacctgcgc cctgggcgcg gccatagccg
     2881 gggcggtggc cgccggggcc tttaaagaca tcccggccgg gcaggaccgc tgctgccgtt
     2941 ttgaagccgc cgattaccgg cccgaaccgg cggcggtccc ggtctaccgg gaactctacc
     3001 ggctctaccg ccagcttcac gacggcttcg gccggccggc cggtccgctg gccggggtga
     3061 tgaaggacct gcttaaattg aagcgcgctt cgaacgcttc ctgagggtgc gtgcgatggc
     3121 tgacctgaga tcggcggttc tcgaggcgaa ccggctgctg ccggaatggg atctggtcgt
     3181 ttccacccag ggcaacgcca gcggttttga tcccggccgg ggccggatga tcatcaagcc
     3241 gagcggggtg ccctacgacc ggctcaagcg gtccgatctg gtcaccctgg atctttccgg
     3301 ccgggtggtg gccggccgcg gccggccgtc ggtcgactgg ccgcaccatc tctttcttta
     3361 ccgggaagtt ccggg
//
LOCUS       DAOQYN010000202         3374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_29526_length_3374_cov_4.661042, whole genome shotgun sequence.
ACCESSION   DAOQYN010000202 DAOQYN010000000
VERSION     DAOQYN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29526_length_3374_cov_4.661042"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /gene="rplF"
                     /locus_tag="PKN80_07625"
     CDS             <1..139
                     /gene="rplF"
                     /locus_tag="PKN80_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393922.1"
                     /note="ribosomal protein L6 appears to have arisen as a
                     result of an ancient gene duplication as based on
                     structural comparison of the Bacillus stearothermophilus
                     protein; RNA-binding appears to be in the C-terminal
                     domain; mutations in the L6 gene confer resistance to
                     aminoglycoside antibiotics such as gentamicin and these
                     occur in truncations of the C-terminal domain; it has been
                     localized to a region between the base of the L7/L12 stalk
                     and the central protuberance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNQ35915.1"
                     /translation="QVGDFAARVRGVRPPEPYGGKGIRYQGEYVRQKVGKTAGATGTT
                     K"
     gene            156..512
                     /gene="rplR"
                     /locus_tag="PKN80_07630"
     CDS             156..512
                     /gene="rplR"
                     /locus_tag="PKN80_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNQ35916.1"
                     /translation="MIKGKEKRRQMRHRRGLKKIQAKAGRPRLMVRKSLKHIYAILID
                     DTAGRVITAVSSHTIKGDAAAGGHKGLAEAVGRLAAEAALKKGFKEVIFDRGGYLYHG
                     NVKILAEAARAAGLKF"
     gene            531..1094
                     /gene="rpsE"
                     /locus_tag="PKN80_07635"
     CDS             531..1094
                     /gene="rpsE"
                     /locus_tag="PKN80_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNQ35917.1"
                     /translation="MAEKRGGVRSSNAPGSRGPGGGPGGRRSFGGGPGGRRAGGRRGG
                     RYAEEGGLYEKVVRINRISKTVAGGRRLRFSALVIVGDRKGKVGVGLSKSHEVPMAID
                     KARRQAQRSMFTIPIEEHTIPNEVLVKFKAAKILLRPAQRGRGIVACQTLRAIAEAAG
                     INDLVTKVIGRSNPVNISKAMMKAFGE"
     gene            1112..1549
                     /gene="rplO"
                     /locus_tag="PKN80_07640"
     CDS             1112..1549
                     /gene="rplO"
                     /locus_tag="PKN80_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNQ35918.1"
                     /translation="MKLNQIPRPRGSKRSTKRLGRGGGSGHGGTSTRGHKGQWSRSGG
                     GVRPGFEGGQMPLIRRLPKFGFTAHPKQTYQVVNLKLLERFPAGATVDEKALAEAGLV
                     RPGLPVKVLAQGELKVALTVKVRAFSKKAAEKIATAGGQAITG"
     gene            1614..2930
                     /gene="secY"
                     /locus_tag="PKN80_07645"
     CDS             1614..2930
                     /gene="secY"
                     /locus_tag="PKN80_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNQ35919.1"
                     /translation="MANLSPFRDSLKIPDLRHKIGVTLLLLAVYRFGCYIPVPGIDGA
                     ALAQFFAQVEGTLLGVADLFTGGALSQATIFALGIMPYISVSIVLELLTTMVPALENM
                     IKSGVEGRRRLTQLTRQLTALLCLFQGMMISLWLENPASFGGVPIVVQPGWAFRIIAT
                     ITLTAGTMFLMWLGEQITEKGIGNGISLIITLGILSRYPTAVKQMLEFLRTGRLSGLT
                     LILLAILIVLVVAATIMLIEGERRIPVQYARRMVGRRQYGGQSTYLPVKVNQGGVIPL
                     IFAVSILLFPATILRFYKGPLAVKLAGYLNPGGWLYTILYALLAVFFCYFYAAVSFKP
                     DNVADDLRKYGGFIAGIRPGKTTGDYLERILLYITFPGALFLVAIALFPNLVMSVFRI
                     PYLVASLFGGVGLLIVVAVLIETMRQIEAHLVMRHYEGFLKKARIR"
     gene            2933..>3374
                     /locus_tag="PKN80_07650"
     CDS             2933..>3374
                     /locus_tag="PKN80_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HNQ35920.1"
                     /translation="MKSDSAASGVVVVFFGPPGVGKGTVATRLAEELKYHFISSGDLL
                     RANVKEGTELGREARSHMDSGGLVPDQLVTRMVLKSLDSLSGGIFLDGFPRTQVQAEE
                     LESYLRGRGWRLQVIDLTADDRFLMKRLVERIICRSCGAIYHRTN"
BASE COUNT          619 a         1064 c         1096 g          595 t
ORIGIN      
        1 gcaggttgga gattttgccg cccgggtacg cggggtccgg ccgccggaac cttacggcgg
       61 caagggaatc cgttaccagg gcgaatacgt ccggcagaag gtcggcaaga ccgccggcgc
      121 caccggaacc accaagtaaa gactggaccg cagacatgat caagggtaaa gagaaacggc
      181 gacagatgcg ccaccggcgc ggcctgaaga agatccaggc caaggcgggc cggccgcgtc
      241 tgatggtgcg caagagcctt aaacatattt acgcgatcct gattgacgac acggcgggcc
      301 gggtgatcac cgccgtctcc agccatacga tcaagggtga cgcggcggcc ggcggacaca
      361 agggcctggc cgaggcggtg ggccggctgg cggccgaggc ggctttgaag aagggtttca
      421 aggaagtgat ctttgaccgc ggcggttatc tttaccacgg caacgtgaaa atcctggccg
      481 aagcggcgcg ggcggccggt cttaaattct aaatatcggc aaaagggaac atggcggaaa
      541 aaagaggcgg ggttcgatct tccaatgctc cgggcagccg ggggccaggc ggcggaccgg
      601 gtggtcggag atctttcggc ggcggaccgg gcggccggag agcgggcggc cggcgtggcg
      661 gccgttatgc cgaggaaggc ggcctctacg agaaggtcgt tcggatcaac cggatcagca
      721 aaacggtggc cggcggccgg cggctgcgct tcagcgccct ggtgatcgtg ggagaccgca
      781 agggcaaggt gggtgtgggc ctttccaagt cgcacgaagt cccgatggcg attgacaagg
      841 cccgccggca ggcccagcgg agcatgttca ccattccgat cgaggaacac accatcccca
      901 acgaggtgct ggtgaagttc aaggcggcca agattctgct gcgccccgct cagcgcggcc
      961 gcgggatcgt cgcctgccag accctgcgcg ccatcgcgga ggcggccggc attaacgacc
     1021 tggtgaccaa ggtgatcggc cggtccaatc cggtcaacat ctccaaggca atgatgaagg
     1081 ccttcggcga ataaaagcac acgggctaaa catgaaacta aatcagattc cgcgtccccg
     1141 gggcagcaaa cggtcgacca aacggctcgg tcgcggcggc ggcagcggcc acggcggcac
     1201 ctccacccgg gggcacaagg gacagtggtc ccggagcggc ggcggagtcc ggccgggttt
     1261 tgaaggcggc cagatgcccc tgatccggcg gcttcccaag ttcggcttca ccgctcatcc
     1321 aaaacagacc taccaggttg tcaacctcaa acttctggaa cgctttccgg ccggcgcgac
     1381 ggtggacgaa aaggcgctgg ccgaggccgg actggtcagg ccgggcctgc cggtcaaggt
     1441 cctggcgcag ggcgaactga aagtcgccct gaccgtcaag gttcgggcct tcagcaagaa
     1501 agcggccgag aagatcgcca ccgccggcgg tcaagccata accggctgat tgatggcgga
     1561 tcgcgtcgca gtccaggggc tttccccgtc cacacaccag aagggttgaa cccatggcca
     1621 acctatcgcc tttccgggac agtctgaaga tacccgacct gcggcacaag atcggggtca
     1681 cgctgttgct gctggcggtt taccgttttg gctgctacat accggtgccc ggtatcgacg
     1741 gcgcggccct ggcgcagttc ttcgcccagg tcgagggcac cctgctgggc gtggccgacc
     1801 tgtttaccgg cggcgccctt tcccaggcga ccatcttcgc cctgggcatt atgccctaca
     1861 tttcggtttc gatcgtcctg gaactgctga ccaccatggt gccggccctg gaaaacatga
     1921 ttaaaagcgg cgtggaaggc cggcgccggc tcacccagct cacccgccag ctgacggccc
     1981 tgctctgcct tttccagggg atgatgatca gcctctggct cgaaaacccg gccagtttcg
     2041 gcggggtgcc gatcgtggtt cagccgggct gggccttccg gattatcgcc accatcaccc
     2101 tgaccgccgg taccatgttc ctcatgtggc tgggcgaaca gatcaccgag aaggggatcg
     2161 gcaacgggat ttcgctgatc ataaccctgg gcatcctttc gcgttatccg accgcggtca
     2221 agcagatgct ggagttcctg cgcaccggcc gcctttccgg cctgacgctg atcctgctgg
     2281 ccatcctgat cgtcctggtg gtggcggcca ccatcatgct gatcgagggc gaacggcgca
     2341 tcccggttca gtacgcgcgg cggatggttg gacggcgcca gtacgggggc cagtcaacct
     2401 acctgccggt caaggtcaac cagggcgggg ttatcccact catcttcgcg gtttccatcc
     2461 tgcttttccc ggccaccatc ctccgttttt acaagggtcc gctggccgtc aagctggccg
     2521 ggtatctcaa ccccggcggc tggctctaca cgatacttta cgccctgctg gccgtcttct
     2581 tctgctactt ttacgcggcg gtcagcttca agcccgacaa cgtggccgac gacctccgca
     2641 agtacggcgg cttcatcgcc ggcatccggc cgggcaaaac gaccggcgac tacctggagc
     2701 ggatccttct ctacataacc tttccgggcg ccctgttcct ggtggcgatc gcgctcttcc
     2761 ccaacctggt gatgagcgtc ttccggattc cctacctggt ggccagcctc ttcggcggcg
     2821 tgggcctgct cattgtcgtg gcggtgctga tcgagacgat gcgtcagatc gaggcccacc
     2881 tggtgatgcg ccattacgag ggctttctca agaaggcccg catccgctga cgatgaaatc
     2941 cgattcggcc gcatccggcg tggtggtggt cttcttcggg ccgcccgggg tcggcaaggg
     3001 cacggtggcc acccggctgg ccgaggaact caaataccat tttatctcct ccggggacct
     3061 gcttcgggcc aacgtcaagg aaggcacgga actgggccgc gaggccagga gccacatgga
     3121 ttccggcggc ctggtgcccg atcagctggt cacccggatg gtcctgaaga gtctggactc
     3181 cctttccggc ggcatcttcc tggacggatt tccccggacc caggttcaag ccgaggaact
     3241 ggagagttat ctgcgcggcc gcggctggcg cctccaggtg attgacctta ccgccgacga
     3301 ccggttcctg atgaaacggc tggtcgagcg gatcatctgc cggagttgcg gagccatcta
     3361 ccaccggacc aacc
//
LOCUS       DAOQYN010000203         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_29706_length_3360_cov_3.710439, whole genome shotgun sequence.
ACCESSION   DAOQYN010000203 DAOQYN010000000
VERSION     DAOQYN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29706_length_3360_cov_3.710439"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKN80_07655"
     CDS             complement(<1..346)
                     /locus_tag="PKN80_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ35921.1"
                     /translation="MRILIIGGGMITREVLIPAVQAFSRRHGRDHLLIASRRGATLQR
                     ISDEFPGLEFQAYPDPACSDPEASRPEAYREALADLEPPAAVIVATPDHLHTGPVLEA
                     IEAGFDCVVQKPL"
     gene            562..1809
                     /locus_tag="PKN80_07660"
     CDS             562..1809
                     /locus_tag="PKN80_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35922.1"
                     /translation="MSSEARVGEYSGRPFICLNCNAPIDRGENIRFTCPRCKSEVLVP
                     PAGNGRSEISLAGQRMSMEADNQVNRISKFPFVADYYGHPLLRAYRREFPFDQMYRGA
                     RDEFAALVNFRDLHRTAWYRERPGIPFLKRYHLRRLTRRDDFWYCTHFARVFTMSAAA
                     LAWPARIVNVMRPVKWTEKAMGHMVVDIWSNQYQKWIYMDVLHNFHYENESGEPLDLL
                     EARDLLFNHRGQGLYLNTLKKKSEGWKPGRYHLGEAKPDDYTRLLQERSLNTFWCLFY
                     HAQDYFTNPLEERRVPILIYRDRLTAGREPVGESRLHYADEPLVVNTANALDIYPTMN
                     NAELQVMVDPKDSPGRLRVYIATQTPNLKQILYRQDGGAWKKYPVDGLVVPMAGRKRV
                     KLEAVTENLAGRRGRPSRLGLSW"
     gene            1835..3067
                     /locus_tag="PKN80_07665"
     CDS             1835..3067
                     /locus_tag="PKN80_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ35923.1"
                     /translation="MKESSRGLNRQVEALEKVARFVDSIGDLRKLLAEIMRVSEEMLE
                     AEASSFLLYDPEKKELYFEIALGAKGGRIRKKRVKLGQGIAGNCAASGKTINIRDVYR
                     DRRFFSGMDARSGFRTTSILAVPVRHKDKLLGVLEILNKRGGRPFNNQDQALAEIIAS
                     QAAVVVENAQLYAQSLKSERMSAIGQTIAGLSHDIKNILAGLQGGVELVDQSLVDEDV
                     DTLQTGWRMVKKNIGKVSDLVLDMLNYARERGPSLRPTNINQVLTEAAGLYAEKLADQ
                     KAACRLALDDRIGTALLDPAGMERAILNLLNNALDALPAEGGLITLASRRSGRKLDID
                     IGDNGCGIPADKISEIFNMFFTTKGSHGTGLGLAVVDKIIREHKGRIRIKSRVGAGTT
                     FTLTLPYREPDDEAEKRG"
     gene            3042..>3360
                     /locus_tag="PKN80_07670"
     CDS             3042..>3360
                     /locus_tag="PKN80_07670"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNQ35924.1"
                     /translation="MTKLKREDKTRPGLPIPAGALLGAGIGLLLFILFQIPLLNSLEL
                     ASLDLRFRSRPPRPAHPAIFLVAINDHSLARIGQWPWSRDLHAQMLEILGKSGARAVG
                     FDIL"
BASE COUNT          684 a         1072 c         1030 g          574 t
ORIGIN      
        1 agagcggttt ctggaccacg cagtcgaagc cggcctcgat ggcctccagt accggaccgg
       61 tgtgcaggtg atccggggtg gccacgataa ccgccgccgg cggttccaga tcggccagag
      121 cctcccggta ggcctccggc cgcgaggcct ctggatcgga gcaggccggg tcggggtagg
      181 cctgaaattc caggccgggg aattcatcgg agattctctg gagggtggcg ccgcgccggg
      241 aggcgatcag gagatggtcg cggccgtgtc ggcgcgaaaa ggcctggacg gccgggataa
      301 gcacttcccg ggtgatcatg ccgccgccga tgatcaggat gcgcatgccg gactccgttt
      361 tcagtgggtt cgccgctttt gaaattccgt ccgtttcaat tttaacacat ccgcctcaaa
      421 accggctacc gggaaagttc cagaataaaa ccccgcctct tgccgcaact tctttttgct
      481 ggtaaaattg atccggtcgg cccgtcgggc atggtgcctg gacgggtgcc ggccattcaa
      541 gccatcgggg atggagcgcc aatgtcatcg gaagccaggg ttggagagta ttcgggccgg
      601 ccgtttatct gcttgaactg caacgcgccg atcgaccgcg gcgaaaatat ccggttcact
      661 tgtccccgct gcaagtccga agtcctggtc ccgcccgccg ggaacggccg cagcgaaatc
      721 agcctggccg gacaacggat gtcaatggag gccgacaacc aggtcaaccg catcagcaag
      781 tttccctttg tcgccgacta ctacggccac ccgctcctgc gcgcctaccg gcgcgaattt
      841 cccttcgatc agatgtaccg cggcgcccgg gacgagttcg ccgccctggt caacttccgt
      901 gacctgcacc ggaccgcctg gtaccgggaa cggcccggga taccctttct caagcgctac
      961 catctccgcc ggcttacccg gcgggacgac ttctggtact gcacccattt cgcgcgggtc
     1021 ttcacaatga gcgcggccgc cctcgcctgg ccggcccgca tcgtcaacgt catgcggccg
     1081 gtcaaatgga cggagaaggc catgggccat atggtggtcg acatctggtc caaccagtac
     1141 cagaaatgga tttacatgga cgtgcttcac aattttcact acgagaacga gtccggcgaa
     1201 cccctggacc tgctcgaggc ccgtgacctg ctcttcaacc accgcggcca gggactttac
     1261 ctgaacacgc tgaagaagaa gagcgaaggc tggaaacccg gccggtatca cctgggcgag
     1321 gcaaaaccgg acgattatac ccgcctgctc caggaacgct cgctgaacac cttctggtgc
     1381 ctcttctacc acgcccagga ttacttcacc aatccgctcg aagaacgccg ggtccccatc
     1441 ctgatttacc gggaccggct gaccgccggg cgggagccgg tgggcgaaag ccgcctccat
     1501 tatgccgacg aacccctggt ggtgaacact gcgaacgccc tggatatcta cccgaccatg
     1561 aacaacgccg aactgcaggt gatggttgac ccgaaggaca gccccgggag gctccgggtc
     1621 tatatcgcca cccagacgcc caacctcaag cagatacttt accggcagga cggcggggcc
     1681 tggaagaagt acccggttga cggcctggtc gtgccgatgg ccgggcgcaa acgggtcaag
     1741 ctcgaggcgg tcaccgaaaa cctggccggc cgccggggcc ggcccagccg cctgggcctc
     1801 agctggtaaa cggacttatt actccgggag aggaatgaaa gagagttcgc ggggtctcaa
     1861 ccgccaggtc gaggcgctgg aaaaggttgc ccgattcgtg gattccatcg gcgacctgcg
     1921 caagctcctg gccgaaatca tgagggtcag cgaagagatg ctggaggccg aggccagctc
     1981 cttcctgctc tacgacccgg agaaaaaaga actctacttc gagatcgccc tgggcgcaaa
     2041 aggcggccgg atccgtaaga aacgggtcaa gctcggccag ggtatcgccg gaaactgcgc
     2101 ggcttccggg aagacgatca acatccggga tgtttatcgc gaccggcgtt tcttctccgg
     2161 catggacgcc aggagcggtt ttcgcaccac ctccatcctg gccgtgccgg tccggcacaa
     2221 ggataaattg ctgggggttc tcgaaatact gaacaaacgc ggcggccgcc ccttcaataa
     2281 ccaggaccag gccctggccg aaatcatcgc cagccaggcg gcggtggtgg tcgaaaacgc
     2341 ccagctctac gcccagagct tgaaatcgga gcggatgtcg gccatcggcc agaccatcgc
     2401 cgggctctcc catgacatca agaatatcct ggccgggctt cagggcgggg tggaactggt
     2461 tgaccagtcc ctggtcgacg aggatgtcga caccttgcag accggctggc ggatggtcaa
     2521 gaaaaacatc ggcaaggtct ccgacctggt gctggatatg ctcaactacg cccgggaacg
     2581 gggacccagc ctgaggccga ccaacatcaa ccaggtgctc accgaggcgg ccggactcta
     2641 cgccgaaaaa ctggccgacc agaaggccgc ctgccggctg gccctggacg accggatcgg
     2701 cacggcgctg ctggatccgg ccggcatgga acgggcaatc ctgaacctgc tcaacaatgc
     2761 cctggatgcg ctgccggccg agggcgggct gatcaccctg gccagccgcc gcagcgggcg
     2821 gaaactggac atcgacatcg gcgacaacgg ctgcggcatc ccggccgaca agatatcaga
     2881 gatattcaac atgttcttca ccaccaaggg cagtcacggt accgggctgg gcctggcggt
     2941 ggtcgacaag atcatccggg aacacaaagg ccggatccgg ataaaatccc gggtgggggc
     3001 cggtaccacc ttcaccctca cgcttcctta ccgagagcca gatgacgaag ctgaaaagag
     3061 aggataaaac ccggccgggg ttgccgatac cggccggggc cctcctgggc gccggtatcg
     3121 gtttgctgct cttcatcctc tttcaaattc cgctcctgaa cagcctggaa ctggcctcgc
     3181 tggatctccg attccgaagc cggccgcccc ggccggccca tccggccatc tttctggttg
     3241 cgattaacga ccattcgctg gccaggatcg gacaatggcc ctggtcccgg gacctgcacg
     3301 cccagatgct cgaaatactc ggaaaatcgg gggcgcgcgc cgtcggattt gatatccttt
//
LOCUS       DAOQYN010000204         3356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_29761_length_3356_cov_4.117540, whole genome shotgun sequence.
ACCESSION   DAOQYN010000204 DAOQYN010000000
VERSION     DAOQYN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29761_length_3356_cov_4.117540"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..618
                     /locus_tag="PKN80_07675"
     CDS             166..618
                     /locus_tag="PKN80_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNQ35925.1"
                     /translation="MVGEHLGYIQRKLGHYPIPTVDAIIETAGGIVLIERKNPPYGWA
                     LPGGFVDWGESLETAVRREAMEETGLELEDLRQFHTYSEPGRDPRFHTITTVFTARGR
                     GEPVARDDAAGIALFPPERLPAVMAFDHRRILQDYFEAKFNPSSTMAK"
     gene            complement(573..1670)
                     /locus_tag="PKN80_07680"
     CDS             complement(573..1670)
                     /locus_tag="PKN80_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35926.1"
                     /translation="MLRVPLENPKPDIDNFIRTVRGETTPDRPPLVELFLDPEVVRNI
                     STNHLGRRWPEKVETDAEKEAYWRQWLEVYWRMGYDFVRVTGGLIFSRKLRRAEDTAG
                     LSRGTRGWAEEGVGMITSWEDFKRYEWPDPVKSDLWNYEFVSSILPEGMGLLVCPTSG
                     FLEITLDHLFGYQNLALMLYDQPDLVAAVFQKVGETICALYERLVGLPGLRGFFQGDD
                     MGFKTGTMIAPDDLRRYVFPWHKKLSELAHQHGLVYLLHSCGRLDRVMEDLIEKVKID
                     GRHSFEDASNPMLEFKRKYGSRVAVLGGVDLDKLCRLEEAQLRGYVRQIIEAAMPGGR
                     FCLGSGNSVANYVPVKNYFAMVEEGLNFASK"
     gene            1975..>3356
                     /locus_tag="PKN80_07685"
     CDS             1975..>3356
                     /locus_tag="PKN80_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35927.1"
                     /translation="MARGFWRRSVFCRSILHPLALSLTLPLFLIPAPGHCQPPYFESG
                     IAFIDHAFFRKADSLDPGTVHLFIKNTGATPLSVEKIILNGTELTGMPDDQILWYQAV
                     PPVSRPGEVIDCMVKLRRPTRKPIRIEARLSNRERVSAVIHPSPPRLKFNFIGFSRNY
                     EAVYLYLENTGKEDLSIEKIFCNARDITPQCFIPNKTFRPSSKGLVIYTPPGPLLQGK
                     YLTFKAIAGGEETAEAQIRVYSHFPITTFGREDTRSEFGFDPAGFEMKYPASEANRNQ
                     PPFKAYHLIDDPACADGKAYQIIGTAAGEIVKRAGECWTVDPIHPNLIYSCEHYKPDS
                     YFIYAETTDIMVIDPYAMYFNRNQPQRDAYFTSIAKRASEPRLLWTIPQAFTAGEKRF
                     PTPEEERIIVWSEVGEGSKGIWYYRYNPRGGYPASPPLEEEIGRINRELQLVRDYILI
                     SEPFPLAAAEA"
BASE COUNT          641 a         1077 c          996 g          642 t
ORIGIN      
        1 ccgggggaac cttccactag aaaggcggcc gtctcctcgg ccatgatccg ggccgcccgg
       61 atcatggccg aggagacggc cgcctttctg gtggaaggtt cccccggcct gcgcgaagtc
      121 cggttcgtgc tcttcgacgc gccgaccttc cggatcttcg agaaggtggt cggcgagcac
      181 ctgggctaca ttcagcgcaa gctcggccat tacccgatcc cgaccgtgga tgccatcatc
      241 gagaccgccg gcgggatcgt gcttattgag cgcaagaatc cgccctacgg ctgggccctg
      301 ccgggcggct tcgtggactg gggggagagc ctggagacgg ccgtgcggcg cgaggcgatg
      361 gaggagaccg gactggagct ggaggatctg cggcagttcc acacctacag cgagccgggc
      421 cgcgacccgc gtttccacac catcaccacc gtcttcaccg cccggggccg gggcgagccg
      481 gtcgcccggg acgacgcggc cgggatcgcg ctctttccgc cggaacggct gccggccgtg
      541 atggccttcg accaccggcg gatcctgcag gattacttcg aggcgaagtt caatccctcc
      601 tcgaccatgg cgaagtagtt tttgaccggc acataattgg ccaccgagtt gcccgagccc
      661 aggcagaagc ggccgcccgg catcgcggcc tcgatgatct gccggacgta accgcgcagt
      721 tgcgcctcct ccagccggca taatttatcg aggtccaccc cgcccaggac cgccacccgg
      781 cttccgtact tccgcttgaa ctccagcatc gggttgctgg catcctcgaa ggagtggcgg
      841 ccgtctatct ttactttctc gatcaggtcc tccatcaccc ggtccagccg gccgcaactg
      901 tgcagcaggt aaaccagccc gtgttgatgg gccagctctg acaacttctt atgccaggga
      961 aagacgtaac ggcgcaggtc gtccggggcg atcatcgttc cggtcttgaa gcccatgtcg
     1021 tcgccctgaa agaatccgcg caatcccggt aagcccacca gtcgctcgta aagggcgcag
     1081 atcgtctcgc cgaccttctg gaagacggcc gccaccaggt cgggctggtc gtagagcatc
     1141 aaagccaggt tctggtagcc gaagaggtgg tcgagcgtta tctcgaggaa accggaggtg
     1201 ggacagacca gcaggcccat gccttccggc aggatggagg agacgaattc gtaattccag
     1261 aggtccgatt tgaccgggtc gggccattcg taacgcttga aatcctccca ggaggtgatc
     1321 atgccgaccc cttcctcggc ccagccgcgc gtgccccggg agaggccggc ggtgtcttcg
     1381 gcccggcgca gtttgcgcga aaagatcagg ccgccggtga cccggacgaa atcgtaaccc
     1441 atccgccagt aaacctccag ccactggcgc cagtaggcct ccttctcggc gtcggtctcg
     1501 accttttccg gccagcgccg gcccaggtga ttggtggaaa tgttccggac gacctccggg
     1561 tcgaggaaga gttccaccag cggcggccgg tccggggtcg tctcgccgcg gacggtccgg
     1621 atgaagttgt caatgtccgg tttcgggttt tccagcggta cgcgcagcat ggcgcctcct
     1681 tttgtgtgcg tatgcagaca ttatatcccc gtccccgttt ttatttccag ccgccgccgg
     1741 gatggcggtg tcgcaagccg ttctccattg gccccggacc gaccggcaac aactgcaggc
     1801 cgacggggct gacatggcgg gcaaaataag gtgggtcggg ataacgggaa gaagccggcc
     1861 cggccttgga gccgggttga caaaaggtct gggggggggg tattataggg ataaacgcgc
     1921 ggcagagtga aggtttttta tgcgcgcccc agaattcttg ccgggaggtg aaacatggcc
     1981 cgcggatttt ggcgacgaag tgttttttgc cggtccattc tccaccccct ggccctctct
     2041 ttaaccctgc cccttttcct gatccccgcg ccgggccact gccagccgcc ctattttgaa
     2101 tccgggattg cctttataga ccacgccttt ttccggaagg ccgactccct cgatccgggc
     2161 accgtccatc tgttcatcaa gaacaccggc gccacccccc tttccgtgga gaagatcatc
     2221 ctgaatggaa cggagctgac cgggatgccc gacgaccaga tcctctggta ccaggcggtt
     2281 cccccggtca gccggcccgg cgaggttatt gactgtatgg taaaactgcg ccggccgacc
     2341 cggaaaccca tccggatcga ggcccggctg agcaaccggg agcgtgtctc ggcagtcatc
     2401 cacccctctc cgccgcggct gaaattcaac ttcatcggtt tcagccggaa ttacgaggcg
     2461 gtctatctct accttgaaaa taccgggaag gaagatttaa gcatagaaaa aatcttctgt
     2521 aatgcccggg atatcacccc ccaatgcttt atcccgaaca agaccttccg gccttcttcc
     2581 aaggggctgg ttatctatac gcctccaggt ccgcttcttc aaggcaagta cctaaccttc
     2641 aaggcaatcg ccggcggtga ggagaccgcc gaagcgcaga tccgggtcta ttcgcacttt
     2701 cccatcacca ccttcggccg ggaggatacc cgctccgagt tcggtttcga ccccgccggc
     2761 ttcgaaatga agtacccggc ttccgaggcg aaccggaacc agccgccctt caaggcctat
     2821 cacctgattg acgatccggc ctgcgccgat ggcaaagcct atcaaatcat aggcaccgcg
     2881 gccggggaga ttgtcaaaag ggccggggaa tgctggaccg ttgaccccat ccaccccaat
     2941 cttatctact cctgcgagca ttataagccc gactcctatt tcatctacgc cgaaacgacc
     3001 gacatcatgg tcattgatcc ctacgcgatg tattttaatc ggaaccagcc gcagagagac
     3061 gcttacttca cctccatcgc caagcgggcc agtgaacccc gcctgttatg gaccatcccc
     3121 caggccttca cggccggcga gaaacgcttt ccgactccgg aagaggaacg gatcatcgtc
     3181 tggtcggagg tgggcgaagg cagtaaggga atctggtatt accggtataa tccgcggggc
     3241 gggtatccgg ccagtccgcc gctggaagaa gagatcgggc ggatcaatcg ggaactccag
     3301 ttggtccgcg attatatcct tatcagcgaa ccctttcccc tggcggcggc cgaggc
//
LOCUS       DAOQYN010000205         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_30770_length_3281_cov_3.169250, whole genome shotgun sequence.
ACCESSION   DAOQYN010000205 DAOQYN010000000
VERSION     DAOQYN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30770_length_3281_cov_3.169250"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PKN80_07690"
     CDS             <1..773
                     /locus_tag="PKN80_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNQ35928.1"
                     /translation="FQVKPRFSVGLGLNYYSGRIKLEALDPYFMMGFPDGSHQLKADG
                     EQWGYNLGLLYRVAENQSFGFAYRGGFPMTFKGDLKMRNLPVPLGGPTYSTPARLDLD
                     YPALVNLGYAWRPRPDLKLEFDLEWTNWSVMDRVPVDFAYNTPFLPDQSTLKDWNDTL
                     SPRLGLEYQAGPNARWRAGYLFLPSPIPDRTYEAGLPDADSHIFNLGFEWKGSRLRLA
                     VSQSLSLPEKRRILQGGPYDGEYRAVNHITTVNLGYDF"
     gene            797..1168
                     /locus_tag="PKN80_07695"
     CDS             797..1168
                     /locus_tag="PKN80_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ35929.1"
                     /translation="MERKNQKKILIIDDEIGPRESLKILFKDNYEVNLAKDGLEGLAT
                     MEKEAYDLVILDLKMSGLGGIEVLRQLREARQQTKVIILTGYGSHEIAQEAQRLGVTA
                     YLNKPFDVFDIRQLVARVINA"
     gene            1161..1742
                     /locus_tag="PKN80_07700"
     CDS             1161..1742
                     /locus_tag="PKN80_07700"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNQ35930.1"
                     /translation="MLKKKDFLETINALLMVIDGKDHYTMYHSLEVMDYAHAFAKELG
                     LPRKEREVIRTAGLFHDIGKIAVPDAILNKPGRLTAEEFERIKQHPVEGAKIIGQVRF
                     LEPVLEMVLSHHERLDGSGYPAGKKAEGLGLGTRILAIADVYSALITDRPYRPAFSRG
                     QAGKVLLGMMEELDQSLVEVFVSRVIRERSGVS"
     gene            1940..2299
                     /locus_tag="PKN80_07705"
     CDS             1940..2299
                     /locus_tag="PKN80_07705"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ35931.1"
                     /translation="MKKQAGFTVIEIITALVIFVVGFIGSAGFFYANRHNLAVANRQR
                     LSYWSALEKLEWLKEQAYADLADSTELVQLNRALEATRSVVVEEINENGALYKRITVT
                     VERGEERVQLVTYAADI"
     gene            2283..2774
                     /locus_tag="PKN80_07710"
     CDS             2283..2774
                     /locus_tag="PKN80_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNQ35932.1"
                     /translation="MRPTSDRRPDSRGYTLVEMVVTLVVGLISVLVIGALLIIFQAEF
                     QNSRKIQDLQADADVVSYNIKGISEEADSETILDSGRRLEVSRGGGWRRAFYPEGEGL
                     VMEDLQTGSKETLTPMLKTILFTSVEPGLVRVDLVLHTWLKNSERELSNSFDVYLRNR
                     ESE"
     gene            2771..>3281
                     /locus_tag="PKN80_07715"
     CDS             2771..>3281
                     /locus_tag="PKN80_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35933.1"
                     /translation="MKVLRQDGYLLPMVIMILLVSFIVGFATLNIGLFERNHANRRLD
                     REAAFYLAEAGLQRACAHLQADFNWQPEPGPLPLGVGSFEVTRQDTGQVITLVSTGSV
                     RNQQERIELNLEASDGLLAGGSFGDGLFGSEWVRIGNNAYIDSYDSRLGPYSLTNRGS
                     DGNVSSNILI"
BASE COUNT          684 a          968 c         1004 g          625 t
ORIGIN      
        1 ccttccaggt caagccgcgg ttttcggtcg ggcttggtct taattactat tcgggccgga
       61 tcaagctgga agccctcgat ccctatttca tgatgggttt cccggacggc agccaccagt
      121 tgaaggcgga cggggaacag tggggttaca acctcggcct gctttaccgg gtggccgaaa
      181 accagtcgtt tggattcgcc taccggggcg ggttcccgat gacgttcaag ggggacctga
      241 agatgagaaa cctgccggtg ccgctcgggg gacccaccta ctccaccccg gcccggctcg
      301 acctggatta cccggccctg gtcaacctcg gttatgcctg gcggcccagg ccggacctga
      361 aactggagtt cgacctggaa tggacgaact ggtcggtcat ggaccgggta ccggttgact
      421 tcgcctacaa tacgccgttc ctgccggacc agtcgacctt gaaggattgg aatgacactc
      481 tcagcccccg gctgggcctg gaataccagg cgggccccaa cgccaggtgg cgggccggct
      541 acctttttct tccctcgccg atacccgacc gcacctacga ggccggtctt ccggatgccg
      601 acagccatat cttcaacctc ggtttcgaat ggaagggcag ccggctccga ctggcggttt
      661 cccagtcact ttcgctgccg gagaagcgcc ggatacttca gggcggacct tacgacggtg
      721 aataccgggc cgtgaatcac atcaccacgg tcaatctcgg gtatgatttc tgatgattac
      781 ggaaatggag gcggtcatgg aaaggaaaaa ccagaagaag atcctgatta tcgacgatga
      841 aatcggcccg cgcgaatccc tcaagatcct tttcaaggac aattacgagg tgaatctcgc
      901 caaggacggc ctggaagggc tggccaccat ggaaaaggag gcgtacgacc tggtgatcct
      961 ggacctgaag atgtccggcc tgggcgggat cgaggttctg cggcagctcc gggaggcgcg
     1021 gcagcagacc aaggtcatca tactcaccgg ttacggctcc cacgaaatcg cccaggaagc
     1081 ccagcggctg ggagtgaccg cctatctcaa caagccgttt gacgtcttcg acatccgcca
     1141 gctggtggcc agggtcatca atgcttaaaa agaaggattt cctcgagacc atcaatgccc
     1201 tgctgatggt catcgacgga aaggatcact acacgatgta ccattccctg gaggtgatgg
     1261 attacgccca cgccttcgcc aaggaactcg gcctgcccag gaaggagcgg gaggtcatcc
     1321 ggaccgccgg tctttttcac gatatcggca agatcgcggt tccggacgcc atactcaaca
     1381 agcccggccg gttgaccgcc gaagaattcg agcggatcaa gcagcacccg gttgaagggg
     1441 ccaagatcat cgggcaggtg cgtttcctgg aaccggtcct ggagatggtg ctttcccacc
     1501 acgagcggct cgacggcagc ggctacccgg ccggcaaaaa agcggaaggg ctgggattgg
     1561 ggacccgcat cctggccatt gccgacgtat attcggccct gatcaccgac cgcccctacc
     1621 gtcccgcctt cagccggggt caggccggca aagtgctgct cggcatgatg gaggaactgg
     1681 accagtccct ggtcgaggtc tttgtcagcc gggtcatccg ggagcggtcc ggggtctcct
     1741 gaagagaagg cgcgggtccg gccttcggat tctcccctga aaaacgacaa gcggcccgcg
     1801 gtccggcctt tctctatttg acaaaccatt tgtcggatag gtattataaa aaacaaggaa
     1861 ccgttataaa tgccatggaa ttaatttaaa gctaatatac tttgtttaat tgtcgaccac
     1921 acaaattcac cgggtgtcca tgaagaagca ggctggcttc accgtaatcg agatcatcac
     1981 cgccctggtg attttcgtgg tgggattcat cggctcggcc ggcttcttct acgccaaccg
     2041 gcacaacctg gcggtcgcca accggcagcg tctttcctac tggagcgccc tggagaagct
     2101 ggaatggctg aaggaacagg cctatgcgga cctggccgat tcgaccgaac tggtccagtt
     2161 gaaccgggcc ctggaggcca ccaggagcgt ggtggttgaa gagattaacg agaacggagc
     2221 tctttacaag agaatcaccg ttaccgtgga acggggtgaa gagcgggtgc agctggtgac
     2281 ctatgcggcc gacatctgac cggcgtccgg acagccgggg ctacaccctg gtcgagatgg
     2341 tggttaccct ggtggtggga ttgatcagcg tcctggtcat cggggccctg ctgatcatct
     2401 tccaggccga gtttcagaac agccgcaaga tccaggatct ccaggccgac gccgacgtgg
     2461 tgagctacaa catcaagggg atcagcgaag aggctgacag cgagacgatt ctcgactccg
     2521 gccgccgcct ggaggtgagc cgcgggggcg gttggagaag ggccttctac ccggagggcg
     2581 aaggcctggt gatggaggac ctgcagaccg gttccaagga gacccttacg ccgatgctga
     2641 aaaccatcct gtttaccagc gtggaacccg gcctggtcag agtggacctg gtgctccata
     2701 cctggctcaa gaattcggag cgggaattga gcaacagctt cgacgtttac ctgcgcaacc
     2761 gggagagcga atgaaagtgc tccgccagga cgggtatctg cttccgatgg tgatcatgat
     2821 tctcctggtc tccttcatcg tcggattcgc caccctcaac atcggcctct tcgagcgtaa
     2881 ccacgcgaac cggcgcctgg accgcgaagc ggccttctac ctggccgagg ccggtttgca
     2941 gcgggcctgc gcccacctcc aggccgattt caactggcag cccgagccgg ggccgcttcc
     3001 cctgggtgtc gggagcttcg aggtgacccg gcaggacacg ggccaggtca tcacgctcgt
     3061 ctccaccggc agcgtccgca atcagcagga gcggatcgag ctcaacctgg aggccagcga
     3121 cggcctgctg gcgggcggct ccttcggaga cggtcttttc ggcagcgaat gggttcgtat
     3181 tggaaacaac gcttatatcg acagctacga ttcccgcctc ggcccttaca gcctgaccaa
     3241 caggggttcg gacgggaacg tcagcagcaa catcctgatc g
//
LOCUS       DAOQYN010000206         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_31004_length_3264_cov_4.941103, whole genome shotgun sequence.
ACCESSION   DAOQYN010000206 DAOQYN010000000
VERSION     DAOQYN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31004_length_3264_cov_4.941103"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="PKN80_07720"
     CDS             <1..1036
                     /locus_tag="PKN80_07720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ35934.1"
                     /translation="AASLPRDEFTPIILTIKPVGPLHRLARQAGIPAHSLRIESRGEA
                     FFALLRLLRFLLAQRPEILHTFLFGANLLGRLAGWTASVPLIISSQRSTDPWRRSYHN
                     LLDKITGPMCRLVVSNSEAGRQMLLKKADLPPEKVITIPNGVETVQPVDRDRARAALG
                     FTPDQVVVGAVGNLRTPKGYQYLLPAFREVYAGHANLRLVIAGEGPLRKDLVSFAQHL
                     GLSSVVSLPGFHKNLRFFYSALDLFVMPSLWEGMPVALLEAMSYGLPVIATSVSGIPE
                     VVRDGQEGFLVEPANPQQIAERLLQLVETPQLRAKLGGRARERVAREFSREQMVQAYA
                     DLYRTLLRTL"
     gene            1169..1798
                     /locus_tag="PKN80_07725"
     CDS             1169..1798
                     /locus_tag="PKN80_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNQ35935.1"
                     /translation="MFGFGELVLKGGILMIPIIFCSILSLAVILERAISLHREQVSSD
                     AFFSKVEEPVRRNNIVEAIELCDQTNGSVPRIVKIGLLRHDRRPEEVREAIEETASFE
                     IPYLERYLGILATVATVSPLLGLLGTVTGLVRAFMVIQMKSGLVNPADLAGGIWEALI
                     TTVAGLVVAIPTYVAYNYFVSRVNAIVTEMEKSASRLIQLLAMREAPAE"
     gene            1816..2226
                     /locus_tag="PKN80_07730"
     CDS             1816..2226
                     /locus_tag="PKN80_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNQ35936.1"
                     /translation="MQTIKFPRHYRITNGQLDMAPLIDMVLNLLIYFMLTSSFLMQPG
                     LKIKLPEAKTTEPGVAKPVVVSVTADDRIFFRDRQVSVEELKNALEIQSRQGHFILLV
                     KGDESARHGLVVQVLDLARLSGAERLIIATSPEI"
     gene            2248..2922
                     /locus_tag="PKN80_07735"
     CDS             2248..2922
                     /locus_tag="PKN80_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNQ35937.1"
                     /translation="MKLFQKALTISFFFHLVLLLVFALALPNRQFQFHQLPVELAPLP
                     APEPPPAPGFMRSDQVRLDGRTGPDAIRIYLPAFASHSEPSASSTEAGTPIVPRLPNL
                     DLIPEAPGGRVDLAIRAPGEDAGLMAPIRTNASLSGPASRRRSVYSPLPRYPNWARER
                     GVEAYLELKFWVEPNGSVRDVEILKSSGYPDLDLVAVGTIGRWRFEAEPVQANWAILP
                     LRFQLR"
     gene            3076..3160
                     /locus_tag="PKN80_07740"
     tRNA            3076..3160
                     /locus_tag="PKN80_07740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3110..3112,aa:Leu,seq:cag)
BASE COUNT          560 a         1154 c          993 g          557 t
ORIGIN      
        1 ggccgcctcg ctgccccgcg acgaattcac cccgatcatc ctgaccatca aacccgtcgg
       61 cccgctccac cggctcgccc gccaggccgg catcccggct cactccctgc gcatcgaaag
      121 ccggggagag gccttcttcg ccttgctgcg cctgctgcgc ttcctgctgg cccagcggcc
      181 ggaaatcctg cacaccttcc tcttcggcgc caacctcctg ggccggctgg ccggctggac
      241 cgcctcggtg ccgttgatca tctccagcca gcgttccacc gatccctggc gccgcagtta
      301 ccataacctc ctcgacaaga tcaccggccc catgtgccgg ctggtggtca gcaactccga
      361 ggccggccgc cagatgctcc tgaagaaggc cgacctgccg ccggagaagg tgatcaccat
      421 acccaacggc gtcgaaaccg tccagccggt tgaccgcgac cgggcccggg ccgccctcgg
      481 cttcaccccg gaccaggtgg tggtgggcgc cgtcggcaac ctgcgcacgc ccaagggtta
      541 ccagtacctg ctgccggcct tccgggaggt ttacgccggc cacgccaacc tgcgcctggt
      601 catcgccggc gaggggcccc tgcgcaagga cctggtcagc ttcgcccagc atctcggatt
      661 gagttcggtg gtctccctgc ccggcttcca caagaacctg cgcttcttct actcggccct
      721 ggacctcttc gtgatgccct cgctctggga gggcatgccg gtcgccctgc tggaggcgat
      781 gtcctacgga ctgccggtga tcgccacctc cgtctccggc atcccggaag tggtgcgcga
      841 cggccaggag ggctttctgg tggagccggc caatccccag cagatcgccg aacgcctgct
      901 ccagctggtc gagacacccc agctccgggc caaactgggc ggccgggccc gggaacgggt
      961 ggcgcgggaa ttctcccggg agcagatggt ccaggcatac gccgaccttt acagaacgct
     1021 tctccgcaca ttataatact tccggccgcc gccaaaaacc ggcggccgta accaggccac
     1081 gcttaagttt cgtccggccg atcaaaaaac ggcgccgtcc ggccgggcga agcaaaaaca
     1141 aacgcaccgg gacggcgccg ccaccgctat gttcggattc ggagaactgg ttttgaaggg
     1201 cgggatcctg atgatcccga ttatcttctg ttcaatcctc tccctggccg tcatcctgga
     1261 acgggccatc agcctgcacc gcgagcaggt gagcagcgac gccttcttca gcaaggtcga
     1321 ggaacccgtc cgccgcaaca atatcgtgga ggcgatcgaa ctctgcgacc agaccaacgg
     1381 ctcggtgccg cgcatcgtca aaatcggcct gctgcgccat gaccggaggc cggaggaggt
     1441 gcgcgaggcg atcgaggaga ccgcctcctt cgaaatccct tacctggaaa gatacctggg
     1501 catcctggcc accgtggcca ccgtctcgcc cctgctgggc ctgctgggca cggtgaccgg
     1561 gctggtgcgg gccttcatgg tcatccagat gaagagcggc ctggtcaacc cggctgacct
     1621 ggccggcggc atctgggagg ccctgatcac caccgtggcc ggcctggtgg tcgccatccc
     1681 gacctatgtc gcctacaact atttcgtcag ccgggtcaac gccatcgtca ccgagatgga
     1741 gaagagcgcc agccggctca tccagctcct cgccatgagg gaagcgccgg ccgaataaga
     1801 gcgcgccgcc atcagatgca gacgatcaag ttcccaaggc actaccggat caccaacggg
     1861 cagctggaca tggcgccgtt gatcgacatg gtgctcaacc tgctgattta cttcatgctc
     1921 acctccagtt tcctgatgca gccgggcctg aagatcaagc tgccggaggc caagacgacc
     1981 gaacccggcg tggccaagcc ggtggtggtt tcggtaaccg ccgacgaccg catcttcttc
     2041 cgggaccggc aggtatcggt cgaggagctg aagaacgcgc tcgaaatcca gagccgccag
     2101 ggtcatttca tcctgctggt caagggcgac gagtcggccc gccacggcct ggttgtccag
     2161 gtgctcgacc tggcccgcct ttcgggggct gaaaggctga tcatcgccac cagtccggaa
     2221 atctgacccg tcagcggccg gccggccatg aaactctttc agaaagcgct gaccatctcc
     2281 ttcttcttcc acctggtcct gctgctggtc tttgccctgg ccctcccgaa ccggcaattc
     2341 cagtttcacc agctgccggt ggaactggcg ccgctgccgg ctccggaacc gcctcccgcc
     2401 ccaggtttca tgcggagcga ccaggttcgc ctggacggcc ggacgggccc ggacgccatc
     2461 cgcatctacc tgcccgcctt cgccagccat tccgaaccga gcgcctccag caccgaggcc
     2521 ggcaccccga tcgtgccccg gctgccgaac cttgacctga tcccggaggc ccccggcggc
     2581 cgggtggacc tggccatccg ggctcccggc gaggatgccg gactgatggc cccgatccgg
     2641 accaacgcct ccctctccgg accggccagc cggcgccgga gcgtctacag tcccctgccc
     2701 cgttatccca actgggcccg cgaacgcggc gtcgaggcct acctggagct caagttctgg
     2761 gtcgaaccga acggaagcgt ccgcgatgtc gagatcctga agagttccgg ctatccggac
     2821 ctggacctgg tggcggtcgg cacgatcggc cgctggcgtt tcgaggccga acccgtccag
     2881 gccaactggg ccatcctgcc tctccgcttc cagctgcggt aatcaagggc gccggccgcc
     2941 gcggcgcgct gaaaccggga gggcggctcc cgttccttcg ggataagtat cttgcctgaa
     3001 agatcaaaac cgggtaaaat atactcttgg tcccgccgcc ggttcgtttt gaggccgcgc
     3061 cgggaccgat taaatgccga agtggtggaa ttggcatacg cgctgcgttc agggcgcagt
     3121 ggggtttatc cccatggggg ttcgactccc cccttcggca ctttggtggc agccctcgaa
     3181 acgagggcac tctcacggtc aagcagttga accacggccg gggagtcgat gggaggccgt
     3241 ttacgaaagg cgaccgatca ggga
//
LOCUS       DAOQYN010000207         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_31149_length_3254_cov_3.421382, whole genome shotgun sequence.
ACCESSION   DAOQYN010000207 DAOQYN010000000
VERSION     DAOQYN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31149_length_3254_cov_3.421382"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /gene="nikR"
                     /locus_tag="PKN80_07745"
     CDS             <1..253
                     /gene="nikR"
                     /locus_tag="PKN80_07745"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNQ35938.1"
                     /translation="GTVTLVYDHHRRELVGELTEIQHGAHRMIISSQHIHLDDDNCLE
                     VVIIRGRAGAVRQLGERLRSVRGVKVGELVMASTGAGLH"
     gene            276..917
                     /locus_tag="PKN80_07750"
     CDS             276..917
                     /locus_tag="PKN80_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNQ35939.1"
                     /translation="MHIPDGFLTPATYLPAWLASAGFLTGFLKRLAGTLQDRAVPLMA
                     VLAAFIFAAQMLNFPVYAGTSGHLLGGVLAAVLVGPYAGAVILSLVLAVQALVFQDGG
                     LTALGANIFNMALAGAFLGYFIYAGLLRLLGPARRLWAAGAAAWFSVVLAAGLCGLQL
                     AISGVAPLGLVLPAMLFIHLFIGLGEALITVAILEAVLRVRPELVYQLEAERP"
     gene            914..1201
                     /locus_tag="PKN80_07755"
     CDS             914..1201
                     /locus_tag="PKN80_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HNQ35940.1"
                     /translation="MKKKEMLLGLLIALLLAAFLSPLASSWPDGLEKVAGQLGFLERG
                     GAAAPAPAPDYLVPGIRSGALSTALAGLAGTLVVFGASLGLAALLRRKRVR"
     gene            1206..1850
                     /locus_tag="PKN80_07760"
     CDS             1206..1850
                     /locus_tag="PKN80_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HNQ35941.1"
                     /translation="MAAMNGKAPAVSPLHRLDPAWKFGAAVAGVLLLTGLPAGARGFF
                     IAYGLALLLLTGLARLSPFYILKKLLAILPFLLLPLVSIPFRPGGPALFRLLLLRSLF
                     SLYTLVIFSATTSFPVLLERLESLRTPRLITVTLALMYRYLFLMAGEVERLKRAVLAR
                     SGRGGSRAILRALAGGTGQLFIRSLERGERVYQAMLSRGYQGLTSKAGSDEKLD"
     gene            1834..2541
                     /locus_tag="PKN80_07765"
     CDS             1834..2541
                     /locus_tag="PKN80_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ35942.1"
                     /translation="MKSLIELRGLSYAYPDGTRALSELDFRICEGERVALIGPNGAGK
                     TTLLLHLNGLILPQAGSVRVDGLEVKRANLPELRRRVGFVFQNPDDQLFLPTIGEDVA
                     FGPRNFGFPEAEVVRRVASALERVGLAGREEKLCFNLSLGEKKRASLATVLAGDPRLL
                     VFDEPTASLDPAGQRQIAGLLSELDLTMLIASHDLVFIRKVCSRVILLDGGRIHADGP
                     AGEILDDGGRLAAHALV"
     gene            complement(2621..3220)
                     /locus_tag="PKN80_07770"
     CDS             complement(2621..3220)
                     /locus_tag="PKN80_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ35943.1"
                     /translation="MKTTRKPCRYFETLKGLMAAIEVTDRKGRRLDLDSGVSRAVELI
                     RELAGAGGQLFFIGNGASAAIASHQASDFWKAGGIPTRAFNDPAMLTSLGNDLGYEEI
                     FARPLLDFARPGDLLVAISSSGRSVNIRRAVQAARRRGCLVLTLSGFKPANPLRGQGD
                     LNFYVPAESYGPVEVCHHAICHCLLDTLVKGGRKTAGRG"
BASE COUNT          412 a         1108 c         1146 g          588 t
ORIGIN      
        1 gggcacggtc accctggttt acgaccacca ccgccgggaa ctggtcggcg agctgaccga
       61 aatccagcac ggggcgcacc ggatgatcat ctccagccag cacatccacc tggatgacga
      121 caactgcctg gaggtggtaa tcatccgggg ccgggccggc gcggtccggc agctgggtga
      181 gcggctgcgt tcggtccgcg gcgtcaaggt gggcgaactg gtgatggcct ccaccggcgc
      241 tggcctgcat tgaccgcatc aaacatgagg agacgatgca catacccgac ggtttcctga
      301 cacccgccac ctacctgccg gcctggctgg cctcggccgg cttcctgacc ggtttcctga
      361 aacggctggc cggcacgctc caggatcggg cggtgcccct gatggcggtg ctggccgcct
      421 tcatcttcgc cgcccagatg ctcaatttcc cggtctatgc cggcacctcc ggccacctgc
      481 tgggcggggt gctggcggcg gtgctggtcg gtccctacgc cggcgcggtg atcctttcgc
      541 tggtcctggc ggtgcaggcc ctggtcttcc aggacggcgg cctgaccgcc ctgggcgcca
      601 acatcttcaa catggccctg gctggcgcct tcctgggcta cttcatttat gccgggctgc
      661 tccgactgct ggggccggcc cggcgcctct gggcggccgg cgcggccgcc tggttttcgg
      721 tggtgctggc cgccggcctc tgcggcctgc agctggccat ttccggggtc gcgcccctgg
      781 ggctggtcct gccggccatg ctcttcatcc acctctttat cgggctgggt gaggccctga
      841 tcaccgtggc catcctggag gcggtgctgc gggtgcggcc cgaactggtc taccaactcg
      901 aggcggaacg gccatgaaga agaaagagat gctcctgggc ctgctgatcg ccctgctcct
      961 ggccgccttc ctttcccccc tggcctcctc ctggcccgac ggcctggaga aggtggccgg
     1021 gcagctcggg ttcctggaac ggggcggcgc cgccgcgccg gcgccggcgc ccgactacct
     1081 ggtgccgggc atccgcagcg gggccctgtc gacggcgctg gccggcctgg cgggcaccct
     1141 ggtggtcttc ggtgcctccc tgggcctggc cgccctgctg cgccgcaagc gggtccgctg
     1201 agaagatggc cgccatgaat ggaaaggcgc cggctgtttc gcccctgcac cggcttgatc
     1261 cggcctggaa gttcggcgcg gccgtggccg gggtcctgct gctgaccggg ctgccggccg
     1321 gggcgcgggg cttctttatc gcctatggcc tggccctgct gctgttgacc ggcctggccc
     1381 gcctttcacc tttttacata ctgaagaaac tcctggccat cctgcccttc ctgctgctgc
     1441 ccctggtttc gatccccttc cggcccggcg gcccggctct cttccggttg ttgctgctcc
     1501 ggtcgctctt ttcactctac accctggtta tcttctcggc caccacctcc ttcccggtgc
     1561 tcctggagcg gctggaatcg ctgcggaccc cgcggctgat caccgtgacc ctggcgctca
     1621 tgtaccgtta cctcttcctg atggccggcg aggtggagcg gctgaaacgg gcggtgctgg
     1681 cccgcagcgg ccggggcggg tcgcgggcga tcctgcgcgc cctggccggc ggcaccggcc
     1741 agctcttcat ccgttccctg gaaaggggcg agcgggttta ccaggcgatg ctgagccgcg
     1801 gttaccaggg gttaacctcg aaggcgggtt ccgatgaaaa gcttgattga attgcgcggc
     1861 ctgagttatg cctacccgga tggcacccgg gcgctttcgg agctcgattt ccggatctgc
     1921 gagggggagc gggtggccct gatcggcccc aacggcgccg gcaagacgac cctgctgctt
     1981 cacctgaacg ggctgatcct tccccaggcc gggtcggtcc gggtggacgg gctcgaggtg
     2041 aagcgggcca acctgccgga gctgcgccgg cgggtgggct tcgttttcca gaacccggac
     2101 gaccagctct tcctgcccac catcggcgag gacgtggcct tcgggccgcg caacttcggc
     2161 ttcccggagg cggaggtggt ccgccgggtc gcttcggccc tggagcgggt gggcctggcc
     2221 ggccgcgagg agaagctctg cttcaacctc agcctcggcg agaagaaacg ggcctcgctg
     2281 gccaccgtcc tggccggcga tccccggctg ctggtcttcg acgagccgac cgccagcctc
     2341 gacccggccg gccagcgcca gatcgccggc ctgctttcgg agctggacct gaccatgctg
     2401 atcgcctcgc acgacctggt cttcatccgg aaggtctgct cccgggtcat cctgctggat
     2461 ggggggcgga tccatgccga cggtccggcc ggcgaaatcc tggacgatgg cggccggctg
     2521 gccgcccatg cgctggtttg agttccccgg ccggattccg ggccggaacg gggaaagaaa
     2581 cggctggaga gagtttaagg ttggtagaag gtggcggagt tcaaccccgg ccggccgtct
     2641 tccggccgcc cttgaccagg gtgtccagca ggcagtggca gatggcgtgg tggcagacct
     2701 cgaccggccc gtagctttcg gccggcacgt agaagttcag gtcgccctgg ccgcgcagcg
     2761 ggttggccgg cttgaagccg gagagggtca ggaccaggca gccgcggcgg cgggcggcct
     2821 ggacggcccg ccggatgttg accgaccggc cggagctgct gatggccacc agcaggtcgc
     2881 cgggccgggc gaagtccagc agcggccggg cgaagatttc ctcgtagccg aggtcgttgc
     2941 ccaggctggt gagcatggcc gggtcgttga aggcccgggt cgggatgccg ccggccttcc
     3001 agaagtcgga ggcctggtgg ctggcgatgg cggcgctggc cccgttgccg atgaagaaga
     3061 gctggccgcc ggccccggcg agttcccgga tcagttcgac cgcccggctg acgccggagt
     3121 ccaggtccag ccgccgcccc ttccggtcgg tcacctcgat ggccgccatc agccccttaa
     3181 gggtctcaaa gtagcggcac ggtttccggg tcgtcttcat ttcaagaggg cggtgatgaa
     3241 cttgtagagg ttgg
//
LOCUS       DAOQYN010000208         3226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_31512_length_3226_cov_5.023652, whole genome shotgun sequence.
ACCESSION   DAOQYN010000208 DAOQYN010000000
VERSION     DAOQYN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31512_length_3226_cov_5.023652"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKN80_07775"
     CDS             <1..452
                     /locus_tag="PKN80_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35944.1"
                     /translation="GVWLALIIFALLSGAMLIREVRGQVYYKLGIMDRNLKSWESAIR
                     NYRRSLELDPANLQAAYKMAYAYGSSGQMARAEATYLEILRVSPNFAKTYYNLALLML
                     QRGDRDSALKYLALSRDYDPYDQESLRLDSLLREGPELFGRPPGGRR"
     gene            565..780
                     /gene="rpmB"
                     /locus_tag="PKN80_07780"
     CDS             565..780
                     /gene="rpmB"
                     /locus_tag="PKN80_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNQ35945.1"
                     /translation="MGKQCVFCGKKRQTGISLSHSHHRTRRFFEVNLQSVRTASGRVL
                     VCTGCLKSGKVIKAAVRSKPKTPAAGS"
     gene            complement(749..2470)
                     /gene="recJ"
                     /locus_tag="PKN80_07785"
     CDS             complement(749..2470)
                     /gene="recJ"
                     /locus_tag="PKN80_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNQ35946.1"
                     /translation="MDNKIWEFVFQDENLKAKLAEELNISPFLASCLINRGITDSRKG
                     RAFLWPELRSLANPMELPGMEAAVERLKTAVRNREKILVYGDYDADGVTATALLSLFL
                     RGTGLEVETYLPHRVEEGYGLHFAPLRAARKRGVTLVVTVDCGSSAAETVRKAVGLGL
                     DIIVTDHHLPGEEVPAEIPVVNPQLGGQDEFRGLSGVGVAFELARALAMALDPADPAG
                     QTEALAEEYLDLVAAGSIADLSPLQGESRILTWFGLRQLRGGRRPAFSQLCAGAGIRP
                     ETIGAQSVGYIIGPRLNAAGRLDSADLALKLFTTSDPEECRLIAAALNDNNRKRQQLE
                     RRIFNEATRAIEDAGLNRQPVLVLAGCDWHLGVVGIVAGKLAERYGKPAIVLSVSSGQ
                     AKGSGRSVSGFNLFEAIQSCQDLLKRYGGHQKAVGLTLETEKLADFTERLNRNSAPLL
                     RTTVQSRVKIELTAELEDLTPEFMRELETLEPFGVENPEPVLATMGLEVQNFSRLVGN
                     NRHLKLWLKRGSVYREAIGFGLGELYPLLPAGVIVDAAYTPRFSDREGVPRISLELKS
                     LRRESSV"
     gene            complement(2460..2708)
                     /locus_tag="PKN80_07790"
     CDS             complement(2460..2708)
                     /locus_tag="PKN80_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35947.1"
                     /translation="MKHAVLLFGKKDCSLCEGWKRKLNHLDIKYDYYDTSTVEGMAEM
                     AFNNVGRIPALVIGPARFEEVGPAEITSEQIQKLIDGQ"
     gene            complement(2708..3187)
                     /locus_tag="PKN80_07795"
     CDS             complement(2708..3187)
                     /locus_tag="PKN80_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNQ35948.1"
                     /translation="MGKVRPGWDQYFMEIARLVATRSTCLRRDVGAVLVRDKRLLATG
                     YNGAPSGLAHCLAIGCLREKLGIPSGERQELCRGLHAEQNAIIQAALHGIPVVGATAY
                     VTCQPCVTCAKMLINCHVKRIVIAGDYPDEFARRMLEEAGVEVVQAETPPTRPKKGK"
BASE COUNT          546 a         1021 c         1024 g          635 t
ORIGIN      
        1 ccggcgtttg gctggcgctg atcatcttcg cgctgctttc cggggcgatg ctgatccggg
       61 aggtgcgcgg gcaggtctat tacaagcttg ggatcatgga ccgcaacctt aaatcctggg
      121 agtcggccat ccggaactac cggcgttccc tggaactgga tccggccaat ctccaggccg
      181 cttacaagat ggcctacgct tacggcagca gcggccagat ggcccgggcc gaagccacct
      241 acctggaaat tctccgggtc agtcccaact ttgccaagac ctactacaac ctggccctgc
      301 tgatgctgca gaggggcgat cgggactcgg cgctcaagta cctggccttg agtcgggatt
      361 acgatcccta tgaccaggag agcctgcggc tggacagcct gcttcgggag ggtccggaac
      421 ttttcggccg gccgccgggc gggcgtcgtt gacggccggg gaataattat gataaaattc
      481 taatttacct taccaccgga atttttccgg ccggcggcgc cgttctgcgc gggtccggtc
      541 ggtacaaatc cgcaaggaaa acccatgggc aaacagtgcg tcttctgcgg caagaaaagg
      601 cagaccggga tctcgctcag ccactcccac catcgcaccc ggcgtttctt cgaggtgaac
      661 ctgcaatcgg tacggaccgc ttccggccgg gtgctggttt gcaccggctg tctgaagagc
      721 ggcaaggtga tcaaggccgc ggtccgttct aaaccgaaga ctcccgccgc aggctcttga
      781 gttccaggga aatgcgcggc acgccttccc gatcgctgaa cctcggcgtg taggcggcgt
      841 cgactatcac ccccgccggc agtagcgggt agagttcccc cagtccaaat ccgatcgcct
      901 cccggtagac acttccccgc ttcaaccaga gtttcaggtg ccggttgttc cccaccagcc
      961 ggctgaagtt ctgcacttcc agccccatcg tggccaggac cggctccggg ttctccactc
     1021 cgaacggctc cagcgtttcc aattcccgca tgaattcggg tgtcaggtct tccagttcgg
     1081 ccgtcagctc gatctttacc cgcgattgga cggtggtgcg cagaagcgga gcggagttcc
     1141 ggttcagccg ttccgtgaaa tcggccagtt tttccgtttc cagcgtcagt ccgaccgcct
     1201 tctgatggcc gccgtatcgt ttcaacaggt cctggcagga ttgaatggct tcgaaaaggt
     1261 tgaagccgga gaccgaccgg ccgctgccct tggcctggcc ggacgagacg gagaggacga
     1321 tggccggctt gccgtaacgt tcggccagtt tgccggccac gatgccgacc acgcccaggt
     1381 gccagtcgca gccagccagg accaggaccg gctggcggtt cagcccggcg tcctcgatgg
     1441 cccgggttgc ctcgttgaag atccggcgtt ccagctgctg ccgtttccgg ttgttatcat
     1501 tcagggccgc ggcgatcagg cggcattcct cgggatcgct ggtggtaaac aatttcaggg
     1561 ccaggtcggc gctgtccagc cggccggccg cgttcagccg gggtccgatg atatagccga
     1621 ccgactgggc gccgatcgtt tccggccgga ttccggcgcc ggcgcagagc tggctgaagg
     1681 cgggccgccg gccgccacgc aactggcgca gtccgaacca ggtcaggatg cggctttcgc
     1741 cctgaagcgg ggagaggtcg gcgatgctgc cggccgccac caggtccagg tactcctcgg
     1801 ccagggcctc ggtctggccg gccgggtcgg ccgggtccag cgccatggcc aaggcccggg
     1861 ccagctcgaa ggccacgccg accccggaga ggccccggaa ttcatcctgg ccgcccagct
     1921 gcgggttgac gaccgggatt tccgccggca cctcttcgcc cggcaggtgg tggtcggtga
     1981 cgatgatgtc cagcccgagt ccgacggcct tccggaccgt ctcggccgca ctgctgccgc
     2041 agtcgaccgt taccaccagc gttactccgc gtttccgggc cgcccgcagc ggagcgaagt
     2101 gcaacccgta accttcttcc acccggtggg gcaggtaggt ctccacctcc aggccggttc
     2161 cgcgcaggaa gagggagagc agggcggtgg cggtgactcc gtcggcgtcg taatcaccgt
     2221 aaaccaggat cttttcgcgg ttgcggacgg ccgttttcag ccgttcgacg gccgcctcca
     2281 tcccgggcaa ttccatcggg ttggccagcg accggagttc cggccagagg aaggcgcggc
     2341 cctttcggga atcggtgatg ccgcggttga tcaggcagga ggccaggaaa ggggagatgt
     2401 ttaactcttc ggccagcttg gccttcaggt tctcatcctg gaagacgaat tcccagattt
     2461 tattgtccat caatcaactt ctggatttgc tccgaggtga tttccgcagg tccgacttct
     2521 tcgaaacggg ccggaccgat gaccagggcc ggtatgcgtc cgacattgtt gaaggccatt
     2581 tcggccatgc cttccaccgt ggaggtgtcg taataatcgt acttgatgtc caggtggttg
     2641 agcttgcgtt tccagccttc gcagagcgaa cagtcctttt ttccgaaaag cagaacggcg
     2701 tgtttcatca ttttcccttt ttcggccggg ttgggggtgt ttccgcctgg accacctcca
     2761 ccccggcttc ttccagcatc cggcgggcaa attcgtccgg atagtcgccg gcgatgacga
     2821 ttcttttgac gtggcagttg ataagcatct tggcgcaggt gacgcacggc tggcaggtga
     2881 cgtaggcggt ggcgccgact accggtatcc cgtgcagggc ggcctgaatg atggcattct
     2941 gttccgcgtg cagtccccgg cagagctcct gccgttcacc ggaggggatc ccgagttttt
     3001 cacgcaggca gccgatggca aggcagtggg ccaggcccga aggcgcgccg ttatagccgg
     3061 tggccagcag gcgcttgtcg cgcaccagca cggcaccgac gtcccggcgg aggcaggtgg
     3121 agcgggtggc caccagccgg gcgatttcca tgaaatactg gtcccaaccg ggacgcactt
     3181 tgcccatcat tttatcttaa cccaaccggg ccggaacggt caaccg
//
LOCUS       DAOQYN010000209         3225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_31530_length_3225_cov_4.598738, whole genome shotgun sequence.
ACCESSION   DAOQYN010000209 DAOQYN010000000
VERSION     DAOQYN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31530_length_3225_cov_4.598738"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PKN80_07800"
     CDS             <1..545
                     /locus_tag="PKN80_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNQ35949.1"
                     /translation="PRYHLSEFWQETARRIFTNLAPELVEREKGLRASGFQPEKFEVL
                     IRAELEPGIILSGKIDRVDASDNSLLLIDYKTGQAPNPGRIGVDYLQLPLYARLYRPA
                     DGRPVGAITIYSLRDPGDLKPITGPDLEEKIQRAVNRAREVVNLIRSGNFRAPENFKN
                     QNCGQCDFSGCCPRRINHAL"
     gene            535..>3225
                     /locus_tag="PKN80_07805"
     CDS             535..>3225
                     /locus_tag="PKN80_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNQ35950.1"
                     /translation="MPCEITFVSAPAGSGKTQRLANAYIALLKRGVEPGRILAITFTE
                     KAAAEMKERILSLLKTNDPPLYRQLLERILEMRVSTIDSFCLSLIRRSAPLIDLSADL
                     EVTTDDRYLREEAFQEALQEIRPRAGRTDEETGRLLEMVTEGRFRGWQAFAERIHYLF
                     EKIHLTSREVYYTPINELLDELGRYSRELVDHPAGPRIFTNYQDLFPPAWTEAAAAQV
                     AGCLRTEQFKLLTEKGEWRKQAPPGSGLKKGAYQNWLEQFKPLARAAWQLESYRRAGE
                     LLNIFRSRFLEAYRKKKWAADLLDFTDLPLLAHQLLTRHPEALNILYAFDEQTDHILI
                     DEFQDTNFLQWGVFEALSEEWFSGASWRNPDQPTSLFMVGDEKQAIYYFRDANSEVFR
                     SIPEELRRREPSFRQETLKHNYRSRAAIIEFTNHLFSQIMTGDPGSGFRVGYLPLEQA
                     RTTKTPGRVELTLLESGPDQENREEVRGREAELASRRILSLVGEFQVSEKDETLRPAR
                     FRDFALLLRERTHLKIFEAGFKKHAIPYTVVKGSGFYQAEEVCLLRSLAAFLADPGDG
                     LALYTLLSSRLFRFQPGEFLRLYDQPGSGWWPKLCAAPEEKARTAVRILEAALARRGR
                     LPVGRLFEEFLLDAEAWRCFPLDPEQVNLKKFLAQLDQFSEQGLSLSEINERLDRLSS
                     DPREARANVLTEDLDAVQVITVHSAKGLEFPIVILPGLDQPFGNKRKEPDLLAMERDP
                     KTFTLAEEPESRLRQFHPLFLENREKELEEERHLFYVAVTRARDALFLSGLRTEKTGE
                     KEKSPLDWIAGALDLDNPPPLPGFRVAGASMILAELETAGPPSPEKAGPPPAAGPDLS
                     APLPAPQPTSRPATDYLEEFRAVSGQARQLGTVIHQLLNEI"
BASE COUNT          652 a         1169 c          909 g          495 t
ORIGIN      
        1 tgccccgcta tcacctgagt gaattctggc aggagacggc ccgccggatc ttcaccaacc
       61 tggccccgga actggtcgag cgcgaaaaag gacttcgggc gagcggcttc cagccggaaa
      121 agttcgaagt cttgatccgc gccgagctgg agccgggcat catcctgagc ggaaaaatcg
      181 accgggtaga cgcctcggac aacagcctgc tcctgatcga ctacaagacc ggccaggcgc
      241 cgaatccggg ccggatcggc gttgattacc tccagttacc cctttacgcc cgcctctacc
      301 ggccggccga cggccggccg gtgggtgcca tcacgatcta ctccctgcgc gatccaggcg
      361 acctcaaacc catcaccggc cctgacctgg aagagaaaat ccagagagcg gtcaaccgag
      421 cccgggaggt cgtcaacctc atccgttccg gcaacttccg cgccccggaa aatttcaaaa
      481 accagaactg cggccagtgc gacttcagcg gctgctgccc gcggcggata aaccatgccc
      541 tgtgaaataa ccttcgtctc ggcgccggcc ggcagcggca aaacccagcg gctggccaac
      601 gcctacatcg ccctgttgaa gcgcggagtc gagccgggcc gcattctggc catcaccttc
      661 accgagaagg cggccgccga gatgaaggaa cgcatcctct ccctgctgaa gacgaatgac
      721 ccgccgctct accggcaact gctcgaacgg atcctcgaaa tgcgggtctc gaccatcgac
      781 tccttctgcc tttcgctcat ccgccgttca gcccccctga tcgacctgag cgccgacctc
      841 gaggtgacca ccgacgaccg ctacctgcgt gaagaggcct tccaggaggc gctccaggaa
      901 atcaggcccc gagccggacg gacggacgaa gagaccggcc ggctgctgga gatggtgacc
      961 gaggggcgct ttcgaggctg gcaagccttt gccgagcgca tccactacct cttcgagaag
     1021 atccacctca ccagccggga ggtttactat acgccgatca acgaactgct cgatgaactc
     1081 ggtcgataca gccgggaact ggtcgatcac ccggccggcc cgcgcatctt caccaattac
     1141 caggacctct tcccgccggc ctggaccgaa gcggcggccg cccaggtggc cggatgcctc
     1201 cggaccgaac aattcaagct gctcactgaa aaaggtgaat ggcgcaaaca ggccccgccc
     1261 ggctccggcc tgaagaaagg ggcctatcaa aattggctcg aacaattcaa gccgctggcc
     1321 cgggccgcct ggcagctgga gagttaccgg cgagccgggg aactgctgaa catcttccgg
     1381 tcccgtttcc tggaagccta ccggaagaag aagtgggccg ccgacctgct ggacttcacc
     1441 gacctgcccc tgctggccca ccagctgctc acccggcacc ccgaagcgct gaacatcctc
     1501 tacgccttcg acgagcaaac cgaccacatc ctgatcgacg aattccagga caccaacttc
     1561 ctccagtggg gcgtcttcga ggccctgagc gaagaatggt tcagcggcgc ctcctggcgg
     1621 aaccccgacc agcccacctc cctcttcatg gtcggtgacg aaaagcaggc catctactac
     1681 ttccgcgacg ccaacagcga agtcttccgt tcaatcccgg aagaactgcg ccgacgcgaa
     1741 ccgagtttcc gccaagaaac gctgaagcac aactaccgga gccgggccgc catcatagag
     1801 ttcaccaatc accttttcag ccaaatcatg accggagacc cgggcagcgg cttccgggtc
     1861 ggctacctgc cgctggaaca ggcgcggacg accaagacgc ccggccgggt cgaactgacc
     1921 ctcctggaat ccggacccga ccaggaaaac cgggaagagg tccgcggccg ggaggcggaa
     1981 ctggccagtc ggcggattct ctccctggtc ggcgaattcc aggtttcgga gaaagacgag
     2041 acgctgcggc cggcccgctt ccgcgatttc gccctcctgt tgcgcgagcg gacccacctg
     2101 aaaatcttcg aggccggctt caaaaaacac gccattccct acaccgtggt caagggaagc
     2161 ggcttctacc aggccgagga agtctgcctg ctgcgcagcc tggccgcctt cctggccgac
     2221 cccggcgacg gcctggccct ctacacgctg ctctccagcc gcctcttccg tttccaaccc
     2281 ggcgaattcc tgcgcctcta cgatcaaccc ggttccggct ggtggccgaa actctgcgcc
     2341 gccccggaag agaaggcccg caccgcggtc cggatcctgg aagcggccct ggcccgccgg
     2401 ggccgcctgc cggtcggccg cctcttcgag gaattcctgc tcgacgccga ggcctggcgt
     2461 tgcttccccc tcgacccgga gcaggtaaac ctgaaaaaat tcctcgccca gctggatcag
     2521 ttctccgaac agggcctctc cctctccgag atcaatgagc ggctcgaccg gctcagttcc
     2581 gacccccgcg aggcccgggc caacgtcctg accgaggacc tggacgcggt ccaggtaatc
     2641 accgtccaca gcgccaaggg cctggaattc ccaatcgtca tcctgcccgg cctcgaccaa
     2701 cccttcggaa acaagcgaaa agaacccgat ctcctggcca tggagcgcga cccgaaaacc
     2761 ttcaccctgg ccgaagaacc ggaatcccgc ctgcgccaat ttcaccccct cttcctggaa
     2821 aaccgggaga aggaactgga ggaggaacgc cacctctttt acgtggccgt cacccgggcc
     2881 cgcgacgccc tcttcctctc cggcctccgc accgaaaaga ccggggaaaa agaaaaatcc
     2941 ccgctggact ggatcgccgg cgcccttgat cttgacaatc caccgccgtt gcctgggttc
     3001 cgggtggccg gggcctccat gatcctggcc gaactggaaa ccgccgggcc gccctcgccg
     3061 gagaaggccg gcccgccccc ggcggccggg ccagacctct cggcgccgct ccccgcgcct
     3121 caaccgacca gccggccagc caccgactac ctggaagagt tccgcgccgt ctccggacag
     3181 gcccggcagc tgggaaccgt catccatcaa ctcctgaacg aaatc
//
LOCUS       DAOQYN010000210         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_31676_length_3215_cov_4.074684, whole genome shotgun sequence.
ACCESSION   DAOQYN010000210 DAOQYN010000000
VERSION     DAOQYN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31676_length_3215_cov_4.074684"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PKN80_07810"
     CDS             <1..603
                     /locus_tag="PKN80_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35951.1"
                     /translation="FRAGLPDLATTIYLPGAALEAGPGTAVLARLYKSYFNQQSWDGY
                     HENLYIPPEKDTGRPALVRYGNICHFSFPIFRGYYQQAVPAYRQLLGNCLDRLHPDPL
                     VRVAGLPSFGQVTVTVQPGGRRLVHLLTYLPELRGPSVQVIEEPILARDLRLELRADG
                     HRPRRIYLAPSGRDLEWTRAGGYLSVRVPEVDGYQLVVFE"
     gene            complement(678..1589)
                     /locus_tag="PKN80_07815"
     CDS             complement(678..1589)
                     /locus_tag="PKN80_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35952.1"
                     /translation="MKRQWLWLAVLICLTFSTSTWADGDQAQQFYRDKKFAEAEAEFR
                     KALAEKPAGPEAAQYQMMIGHSLNGQNKYAEAATEFRKVAEMKDVPATIRLNSALGVG
                     RALYLQKKYDEAIAEYRKILSLEGTRDVNRGEIYYAVGNILAEQKKAPEAAAEYRKIL
                     ELKEAPPHHKSQAYTGLANLLRAEKKYPEAVEELRKISAIESITARQTVPAQIQIATL
                     LLEDRKPDQALAEYRKALTTPEITDPQRAQLLNASGELLQSQGKYEAAIADYRKILEL
                     KDVPDKTKQKGQANIAACEEKLKAGQK"
     gene            1792..2658
                     /locus_tag="PKN80_07820"
     CDS             1792..2658
                     /locus_tag="PKN80_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ35953.1"
                     /translation="MYVAIRDGILKSAGYESIRSGLDDLKLANLELEFFRDYSVYRPD
                     AWEKQTFAPEAAAGAIRDLFGPAGVRVSAFLLHNNFNSADPDAEVRWVIDVIKTAQAL
                     SVPAIRIDAVTKGEKEESFEVRVNRFVECMKAVLAAAPAAGVQLGIENHGIQGNDPEF
                     LRQVIGRVGDPRLGITLDTGNFYWSGLPLSEVYRVFEEMAGQVKHTHVKNIRFPEEIR
                     ERRREPGLDYKIYVSPIGEGDIDHSRLVRILARAGYAGPLTIEDESLGKFELPARREV
                     LKRDVAHLAGLL"
     gene            complement(2681..>3215)
                     /locus_tag="PKN80_07825"
     CDS             complement(2681..>3215)
                     /locus_tag="PKN80_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35954.1"
                     /translation="KDLHDNSRASVLLSTARILRRQEKLDEAEAEYRKIDGLKEITAH
                     YRVSTLTALANLLRDRKKFPEAITEYRKALAVEGIVPVQQASIQMNIAGIVNLQGDKE
                     AAVAEYRKAAAIEGAGDSQRATAYFEAGDLLKSLKKYDEASAEYRKVLALKETSEANR
                     KRAEGSIAACEKLKAGA"
BASE COUNT          506 a         1042 c          984 g          683 t
ORIGIN      
        1 ttccgggccg gcctgccgga cctggccacc accatctacc tgcccggcgc ggccttagag
       61 gccggtccgg gcacggccgt cctggcccgc ctctacaagt cctacttcaa ccagcagtcc
      121 tgggacggct accacgagaa cctctacata ccgccggaga aggataccgg ccggccggcc
      181 ctggtccgat acggcaacat ctgccacttc agttttccga ttttccgggg ttattaccag
      241 caggcggtgc cggcctaccg gcagctgctc gggaactgtc tggaccggct gcatcccgat
      301 ccgctggtcc gggtcgccgg cctgccttcc ttcggccagg tgaccgttac cgttcagccc
      361 ggcggccgcc ggctggttca cctgctcacc taccttccgg agctgcgcgg gcctagcgtc
      421 caggtcatcg aggaaccgat tctggcgcgc gaccttcggt tggaactgcg ggccgacgga
      481 catcggccgc gccggatcta cctggccccc tccggccggg accttgaatg gacgcgggcc
      541 ggcgggtacc tctcggtccg ggtgccggag gtcgacggct accagctggt ggtcttcgaa
      601 taggcgcctt ttgcaaaaaa aaaagagccg ttccccctta ttcagggaac ggccccggtc
      661 cggacggcct caaagcttta tttctgaccg gccttcaatt tttcctcgca ggcggcgata
      721 ttggcctgcc ctttctgctt cgtcttatcc gggacatcct tgagttccag gatctttcga
      781 taatcggcga tggccgcctc gtacttgccc tgcgattgga gcagctctcc ggaggcgttc
      841 agaagctggg cgcgctgggg atcggtgatt tccggcgtcg tcagggcctt ccggtactcg
      901 gccagcgcct ggtccggctt tctgtcctcg agcagcagcg tggcgatctg aatctgggcc
      961 ggaactgttt gcctggccgt gatgctttct atggcggata ttttccggag ttcctcgacc
     1021 gcctccggat actttttctc cgccctcagc aggttggcca ggccggtata ggcctgtgac
     1081 ttgtggtgcg gcggcgcctc cttcaattcc aggatcttcc ggtattcggc cgcggcttcc
     1141 ggcgccttct tctgttcggc caggatattt ccgaccgcgt aataaatctc tccccgattg
     1201 acatcccgcg ttccctccag ggagaggatc ttccggtatt cggcgatcgc ttcatcatac
     1261 ttcttctgca ggtagagcgc ccggccgacg cccagggcgc tgttaagccg gatcgtggcc
     1321 gggacatcct tcatctcggc caccttgcgg aattcggtcg ccgcctcggc atacttgttc
     1381 tgcccgttca gcgagtggcc gatcatcatc tggtactggg cggcctccgg gccggccggt
     1441 ttttccgcca gcgccttccg gaattcggcc tccgcctcgg cgaacttctt atcccggtag
     1501 aactgctgcg cctgatcgcc gtcggcccag gtcgacgtgc tgaaggtcaa gcagatcagg
     1561 acggccagcc acagccattg tcttttcata aggatcacct tagttttttc cggccaggag
     1621 cattcccgcg ggaaatcgat tcccccaacc cggtcataag gtagcacttt ccggtttttt
     1681 tcgtcaaccg ccggtttccg gcctccattt gtcgagaacg gccgccgcgg tttacaattt
     1741 accggtgaag ttctcgacga aagtgtcagg ttcgcaacca ggaggcgcaa gatgtacgta
     1801 gcgatcaggg acggcattct gaaatcggcc ggttacgaaa gcatccggtc cggattggac
     1861 gatttgaaac tggccaatct tgagctcgaa ttcttccgtg attacagcgt ttaccggccc
     1921 gacgcctggg agaagcaaac cttcgccccg gaggcggccg ccggcgccat ccgggacctt
     1981 ttcgggccgg ccggtgtcag ggtcagcgcc ttcctgcttc acaacaactt caacagtgcc
     2041 gacccggacg ccgaggtgcg ctgggtgatc gacgtcatca agaccgccca ggccctctcg
     2101 gtgccggcga tccggatcga cgcggtgacc aagggggaga aggaggagtc gttcgaggta
     2161 cgggtgaacc gtttcgtgga gtgcatgaag gcggttctgg ccgccgcccc ggcggccggt
     2221 gtccagctgg gcatcgagaa ccacggtatc cagggcaacg acccggaatt cctgcgccag
     2281 gtaatcggac gggtgggcga tccgcgcctg ggcatcaccc tggacaccgg caacttctac
     2341 tggtccggat tgccgctcag cgaggtttac cgtgtcttcg aggagatggc cggacaggtg
     2401 aaacacaccc atgtcaagaa tatccgcttt cccgaggaga tccgagagcg gcgccgggag
     2461 cccggtctgg attacaagat ctatgtcagc ccgatcggcg aaggcgacat tgaccacagc
     2521 cggctggtac ggattctggc ccgggccggt tacgccggac cgctgaccat cgaggacgag
     2581 tcgctgggca agttcgagct tccggcccgg cgcgaggtgc tgaagcggga cgtcgcccac
     2641 ctggccggcc tgctctgaac ccggccggca acggatccgt tcaggcgccg gccttcaatt
     2701 tctcgcaggc ggcgatggat ccctcggccc gtttccgatt ggcttcggag gtctccttca
     2761 aagcgaggac cttccggtat tcggcgctcg cctcgtcgta cttcttcagc gacttgagga
     2821 gatcgccggc ctcgaaatag gcggtcgccc gctgcgaatc gccggcgcct tcgatggcgg
     2881 ctgccttccg atactcggcg acggccgcct ccttatcgcc ctggaggttg actatcccgg
     2941 cgatgttcat ctggatcgag gcctgctgga ccgggacgat tccctcgacc gccagcgcct
     3001 tccggtactc ggtgatcgcc tccgggaatt tcttccggtc gcgcagcagg tttgccagtg
     3061 cggtcagggt cgagacccgg tagtgggcgg tgatctcctt cagcccgtcg attttccggt
     3121 actcggcctc cgcctcgtcc agtttttcct ggcgccgcag gatccgggcg gttgaaagca
     3181 agaccgaggc ccggctgttg tcgtgcaggt ccttc
//
LOCUS       DAOQYN010000211         3207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_31797_length_3207_cov_3.580266, whole genome shotgun sequence.
ACCESSION   DAOQYN010000211 DAOQYN010000000
VERSION     DAOQYN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31797_length_3207_cov_3.580266"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            634..2439
                     /locus_tag="PKN80_07830"
     CDS             634..2439
                     /locus_tag="PKN80_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNQ35955.1"
                     /translation="MNPEFLPATPEEMKARGWSEPDILLVTGEAYVDHPSFGAALIGR
                     CLEAAGYRVAVLPLPDWRRSESFAAFGRPRLMVGITAGSLDSMLANRSPGGGRRKTDA
                     YAPGGRPGLRPDRAAIVYANRVREALPGVPILLGGIEASLRRLAHYDFWSDRVRRSIL
                     LDSRADWIAYGMAEAAVLEIAGRLAAGARPEAVRDVRGTVWATSDASELPPRSIILPG
                     ESEVMEDPEAFNQAFRLWYRELDPRSARPVVQPAGNRFVVQLPPPRPLAEPELDGLYR
                     LPFTRRSHPRYDRDGGVPALRTVETSVTSHRGCGGGCSFCTLSAHQGRLVQSRSPESV
                     REEVAGLAGRPGFSGTISDIGGPTANMYGAFCRLGSGGECRRDSCLHPSICPNFAFDH
                     RRHLALLESARRVRGVRHVFVGTGVRHDLVLADPDSGYLDELCRHYVGGQLKVAPEHV
                     VPGVLKLMHKPEPGSFERFKGEFEAAGRRAGRKLYLVPYFISGHPGCTVEDMVALAGY
                     LARLGHFPEQVQEFLPLPMTLSAAMYWTGRDPFTGKPLHVARGEERRLQRALLQPADP
                     RHRRRLEPLLRQRGKAGLLRPSRASQAVKARKRGR"
     gene            complement(2406..>3207)
                     /locus_tag="PKN80_07835"
     CDS             complement(2406..>3207)
                     /locus_tag="PKN80_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HNQ35956.1"
                     /translation="MLARWIEAPFEEISYTCAGINHLAWFTRYEWKGRDAYPLIREAV
                     TTRPDIYNQEQVRNEMLLALDYYVTESSGHNSEYNWWFRKRPELIEKYCTHGTNWNPG
                     VYAYILNNYRRREKTWKADVLGWLEKPEPIKLERGQEYAAYIINALVGGEPYEFNGNV
                     PNRGLVDNLPDGACVEVPVRADHQGLTPRPVGKLPPQCAILTGLQAQVEELAVEGALT
                     GNPHLVYQAVCHDPLTAAVLSLAEIRELVNRMLEFNRPYLPRFRAFTA"
BASE COUNT          476 a         1102 c         1074 g          555 t
ORIGIN      
        1 ggggagggaa gctccttccc ctgaaagcga gccccgaaac tcggcgcggc ttgaagggtc
       61 aatacggata ggctacaata aaaagtcttg aaagatagag aagcaatagt acgtttcggg
      121 gcgtagagat ttcgccgaga ctttggttcg cgccgcggtg ctgcgcgctg gacaaaacta
      181 cgccgctcgg caacgagcct cgccgtgttc ccggtcctgg taagacgggc cgggccccgg
      241 ctactcattg cctcgcacgg cttattttgt tgccatcgct acgcaagcgc cgctcgccgc
      301 ccccctcgct taagggaagc ataaaagaaa ataacggccg ttttggtaac tcaataatat
      361 gaaacttgaa tttctgccgg ctaccccaga agaaatgagg gcgcggcggt ctttggttcg
      421 cgcccactct gcgtccgggg caaaagctac gcgactcggg aacgaggact ctccgtgttc
      481 gcggccctgg taaagcgggc cgcgccccgg atactcattc cctcgtttcg cttgttttgc
      541 acccggacta cgcgacgccg ctcaccgaag gccgccgctt caggagaaaa tataagcgtt
      601 gcggtttccg cggcgctttg ctgaattaac aatatgaatc ctgaattcct gccggccact
      661 cccgaagaaa tgaaggcgcg gggatggagc gaacccgaca tcctgctcgt caccggtgag
      721 gcctatgtcg atcacccctc cttcggcgcc gccctgatcg gccgctgcct ggaggcggcc
      781 gggtaccggg tggcggtgct gcccctgccc gactggcgcc ggtccgagtc cttcgccgcc
      841 ttcggccggc cccgcctgat ggtcgggatc acggccggct ccctcgactc gatgctggcc
      901 aaccgttcgc ccggcggcgg ccgccgcaag acggatgcct acgccccggg cggccggccg
      961 ggcctgcgtc cggaccgggc cgccatcgtc tacgccaacc gggtgcggga ggccttgccc
     1021 ggggtgccga tcctgctggg cggcatcgag gcctcgctcc gccgcctggc ccactacgac
     1081 ttctggagcg accgggtgcg ccgttccatc ctgctggaca gccgggccga ctggatcgct
     1141 tacggcatgg ccgaggcggc cgtgctggag atcgccggcc gcctggccgc cggcgcccgt
     1201 ccggaggcgg tccgagacgt ccgcggcacc gtctgggcca cctccgacgc ttcggagctg
     1261 ccgccccgga gcatcatcct gcccggcgag tcggaggtga tggaggaccc ggaggccttc
     1321 aaccaggcct tccgcttatg gtaccgggag ctggatcccc gttcggcccg gccggtcgtc
     1381 cagcccgccg gcaaccgctt cgtggtccag ctgccgccgc cccggccgct ggcggaaccg
     1441 gagctggacg gactctaccg gctgcccttc acccggcgca gccacccgag gtacgaccgc
     1501 gacggcggcg ttccggccct caggacggtc gagacctcgg tcaccagcca ccgcggctgc
     1561 ggcggcggct gctccttctg caccctgtcg gcccaccagg gccgcctggt ccagagccgc
     1621 tccccggagt cggtccggga ggaggtggcc ggcctggccg gccgccccgg cttctccggc
     1681 accatcagcg acatcggcgg cccgaccgcc aacatgtacg gggccttctg ccgactgggt
     1741 tccggcgggg agtgccgccg ggacagctgc ctgcatccct cgatctgccc gaacttcgct
     1801 tttgaccacc ggcgtcacct ggccctgctg gaatccgccc ggcgggtcag gggagtccgc
     1861 catgttttcg tggggaccgg ggtgcgccac gacctggtcc tggccgaccc ggattcggga
     1921 tacctggacg agttgtgccg ccattacgtg ggagggcagc tgaaggtggc cccggagcat
     1981 gtcgtgcccg gtgtcctgaa gttgatgcac aagccggaac cggggagttt cgagaggttt
     2041 aaaggagagt tcgaggcggc cggccgccgg gccggccgga agttgtacct ggtgccctac
     2101 ttcatctccg gccatccggg ctgcaccgtt gaagacatgg ttgcgttggc cggctacctg
     2161 gcccggctcg gccactttcc cgagcaggtc caggagtttc tgcccctgcc gatgaccctt
     2221 tcggccgcca tgtactggac cggccgggac cccttcaccg gcaagccgct ccacgtggcc
     2281 cgcggcgagg agcgccgcct gcagcgggcc ctgctccagc cggccgaccc gcgccaccgg
     2341 aggcgcctgg agccgctgct gcgtcagcgg ggcaaggccg ggctgctgcg gccttcccgc
     2401 gccagtcagg cagtgaaggc gcggaagcgg ggcaggtagg gccggttgaa ctccagcatc
     2461 cggttgacca gctcccggat ctcggccagg gagaggaccg cggccgtgag cgggtcgtgg
     2521 cagacagcct ggtaaaccag gtgcggattc ccggtgaggg ccccctccac cgccagctcc
     2581 tcgacctggg cctggaggcc ggtcaggatg gcgcactggg gcggcagctt tcccaccggc
     2641 cggggcgtca gtccctggtg gtcggcccgc accggcacct cgacgcaggc cccgtccggc
     2701 aggttgtcaa ccaggccccg gttcggtacg ttcccgttga actcgtaagg ttcgcctccg
     2761 accagggcgt tgatgatgta agccgcgtac tcctggccgc gctccagctt gatgggttcc
     2821 ggcttctcca gccagccgag gacatccgcc ttccaggtct tctcccgccg gcggtaattg
     2881 ttcaggatgt aggcgtaaac cccggggttc cagttggtgc cgtgggtgca gtacttctcg
     2941 atcagctccg gccgcttccg gaaccaccag ttgtactcgg agttgtggcc gctggactcg
     3001 gtgacgtagt aatcgagggc caggagcatc tcgttgcgga cctgctcctg gttgtaaata
     3061 tcggggcggg tggtgaccgc ctcccggatc agcgggtagg cgtcccgccc cttccattcg
     3121 taccgggtga accaggcgag gtggttgatg ccggcgcagg tatagcttat ctcctcgaag
     3181 ggcgcctcga tccaccgggc cagcatg
//
LOCUS       DAOQYN010000212         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_32308_length_3173_cov_2.956703, whole genome shotgun sequence.
ACCESSION   DAOQYN010000212 DAOQYN010000000
VERSION     DAOQYN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32308_length_3173_cov_2.956703"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PKN80_07840"
     CDS             <1..1215
                     /locus_tag="PKN80_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ35957.1"
                     /translation="ILARREPPIVRGVENQRRDRAEVSPYQIPFRSLYSRDIRNLLLA
                     GRNISATHVALSSTRVMGTCGLLGQASGTAAWLCRKHRLEPREIHSARTQELQQALLR
                     DGVFIPGVVNEDKGDLLRGARVTASSQAPLALPPSDGEWELDRPRGQVLPLAGRVGRL
                     AIPLRTKVPARLEFRLERLSDFWDRSGGKLVCEAGDLPVSPRDRCLSVALERELEPGF
                     YRFSLAPAAGVFLKKVLAPLPALYAAERPGAAWRPCPLFQVDFRLAPDIYPFGPGNTV
                     SGVNRPEKWTNLWISDPAAGLPQRLEWEPARPAPIGYLQFFFEAALDLEYKQCPPFFV
                     PAGIPSRYRVSLLDGNRWKRVVEVKSNTEYLRRHDFAPRPAGKVRLEILEVNGGRSAV
                     VTEARGYRRIPD"
     gene            1289..1651
                     /locus_tag="PKN80_07845"
     CDS             1289..1651
                     /locus_tag="PKN80_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35958.1"
                     /translation="MPESNPELKEQIRKIAGSEKLSEAEQDRLIGGLLGSSLPESGPE
                     SGLVHLAFVVSFLVPFSGILIGSHYLIRYGDEGLKPGLICLGLTLVAFLIHFFLLRGL
                     LAFLAGGSGPLPPLPAAG"
     gene            1680..3089
                     /locus_tag="PKN80_07850"
     CDS             1680..3089
                     /locus_tag="PKN80_07850"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNQ35959.1"
                     /translation="MVNESRVRRLNRAAGRPGPVLYWMSRDQRVQDNWALLYAWQAAR
                     ASKAPLAVICCPGADLEGASVRQGDFRLAGLKETARQLESKRIPLFVLDGSPAAAGLA
                     FVRSSGAGALVLDFSPLRPGRAWREETARRLDRPVYEVDAHNIVPAWRASSRQEYAAY
                     TIRPKLARLMDEFLEEFPAVSVRQVIAWPSEVPVIDWDLLRRRLRLDAAVSGSGRFRP
                     GGRAGELVMREFLRDKLARYPVDRNDPNADVQSNLSPYLHSGQISAQRVALETLEAGR
                     ASKASEIFLEELVTRRELAENYCFYNAAYDRPEGFPEWARRSLEKHRSDRRRYLYRRP
                     ELEAGETHDRIWNTAQRQLAGLGKLHGYLRMYWAKKILEWSPDPARAQAEAVYLNDRY
                     ALDGRDPNGYAGIAWSIGGVHDRPWFERPVYGQIRYMNESGCRRKFDVDRYLASGPAG
                     SDPDSGRVETSTRDGRRIR"
     gene            3086..>3173
                     /locus_tag="PKN80_07855"
     CDS             3086..>3173
                     /locus_tag="PKN80_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNQ35960.1"
                     /translation="MRTIILLTISNIFMTFAWYGHLKYKNSPL"
BASE COUNT          506 a         1017 c         1044 g          606 t
ORIGIN      
        1 attctggccc gccgggaacc gcccatcgtc cggggggttg aaaatcaacg gcgcgaccgg
       61 gccgaagtgt ccccttacca gatacccttc cggtcccttt attcccggga tatccgcaac
      121 ctgctcctgg ccggccggaa catcagcgcc acccacgtgg ccctgagcag cacccgggtc
      181 atgggtacct gcggcctgct cggccaggcc tccggtacgg ccgcctggct ctgccggaag
      241 caccggctgg aaccccggga gatccattcg gcccggaccc aagaactgca gcaggcactc
      301 ctgcgggacg gggtcttcat tcccggggtt gtcaacgaag ataagggcga cctgctgcgg
      361 ggagcccggg tgacggcctc ctcccaggcg ccgctggctc tgccgccttc cgacggcgaa
      421 tgggaacttg accggccgcg cggccaggtg ctgcccctgg ccggccgggt gggccggttg
      481 gcgattcccc tgcggacgaa ggttccggcc cgcctcgaat tccgactgga acgcctctcc
      541 gatttctggg accgttccgg cggcaagctt gtctgcgagg cgggagacct tccggtctcg
      601 ccccgggacc gctgcctttc ggtggccctg gagcgggaat tggaaccggg tttttaccgt
      661 ttttcactgg cgccggccgc cggggtcttt ctgaaaaagg tcttggcccc gcttcccgct
      721 ctttacgcgg ccgaacggcc gggggcggcc tggcggccct gtccgctttt ccaggttgat
      781 ttccggctgg cgccggatat ttaccccttc ggccccggga ataccgtttc cggggtcaac
      841 cgaccggaaa aatggaccaa tctctggatt tcagatccgg ccgccggtct tccccagcgg
      901 ctggaatggg agccggcccg gccggccccg atcggttacc tgcagttttt tttcgaagcg
      961 gccctggacc tggagtacaa gcagtgcccg ccttttttcg tgccggccgg aataccctcc
     1021 cgctaccggg tcagcttgct tgacggcaat cgttggaagc gggtggtcga ggtgaagagt
     1081 aataccgagt acctgcggcg ccacgatttc gcgccccggc cggccgggaa ggtccggctg
     1141 gagatcctgg aggtcaacgg ggggcggagc gccgtcgtta ccgaggcccg cggctaccgg
     1201 cggatcccgg attgacaacc gggacggcag ccggtatgat gataagccgg atctattata
     1261 aaaacaggcg gtcggccgga ggacggacat gccggagtca aacccggaac tgaaggaaca
     1321 gatcaggaag atcgccggtt ccgagaagct gagcgaggcg gagcaggacc ggttgatcgg
     1381 cggcctgctc ggcagttccc tgccggaatc cggcccggaa tcgggcctgg tccacctcgc
     1441 ctttgtcgtc agcttcctgg ttccattttc agggattctg atcggcagcc attacctgat
     1501 tcgttacggc gacgagggcc tcaagccggg cttgatttgc ctggggttga ccctggtggc
     1561 cttcctgatt catttttttc tgctgcgtgg attgctggcc ttccttgccg ggggcagcgg
     1621 tccgctcccg cccctgccgg ccgccggttg actccggcct catttcacaa ggtttgaaga
     1681 tggtgaacga atcgcgcgtg cgccgcttga accgggccgc cggccgcccg ggcccggttc
     1741 tttactggat gagccgcgac cagcgggtgc aggataactg ggcccttctg tacgcctggc
     1801 aggcggcccg cgcctcgaag gcgccgctgg cggtaatctg ctgcccgggg gcggatctcg
     1861 agggtgcctc ggtccgccag ggcgatttca ggctcgcggg attgaaggag accgcccggc
     1921 agctggagtc gaagcggata cccttatttg ttctcgatgg atctccggcg gcggcgggcc
     1981 tggccttcgt ccggtcgtcc ggtgccggcg ccctggtcct cgatttctcg ccgctgaggc
     2041 ccggccgggc ctggcgggag gagacggccc ggcgcctgga ccggccggtc tacgaagtgg
     2101 atgcccacaa tatcgtgccc gcctggcggg cttcgagccg gcaggagtat gccgcctata
     2161 ccatcaggcc caaactggcc cgtttgatgg acgagttttt ggaagagttt ccggccgttt
     2221 cggtccgaca ggtgattgcc tggccatccg aagttccggt tatcgactgg gaccttttgc
     2281 gccgccgcct ccggcttgac gcagccgtat ccggctcggg tcggttccgg cccggcgggc
     2341 gggccggaga actggtcatg cgggaattcc tgcgcgacaa gctggcccgc tacccggttg
     2401 accgcaacga tcccaacgcc gacgtccagt cgaacctctc cccctacctt cattccgggc
     2461 agatttcggc ccagcgggtc gcgctggaga cactggaggc cggccgggcc tcgaaggcca
     2521 gcgaaatttt cctggaggag ttggtgaccc ggcgtgaact ggccgaaaat tattgttttt
     2581 acaatgccgc ctacgatcgt ccggaaggtt ttccggaatg ggcgcgccgg agtctggaga
     2641 agcatcgttc cgaccgccgc cggtatcttt accggcgccc ggagctggag gcgggcgaaa
     2701 cccacgaccg gatctggaat accgcccagc gtcaacttgc cggtctgggc aagctgcacg
     2761 gctacctgcg catgtactgg gcaaaaaaaa tactggagtg gtccccggac ccggcgcgtg
     2821 cccaggccga ggccgtttac cttaacgacc gctatgccct ggacggccgc gatcccaacg
     2881 ggtacgccgg gatcgcctgg tcgatcggag gcgttcacga ccgcccctgg tttgaacggc
     2941 cggtctacgg acagataagg tacatgaacg agtccggctg ccggagaaaa ttcgacgttg
     3001 accgttacct ggcctccggg ccggccggct ccgatccgga ttcgggtcgg gtcgaaacct
     3061 cgacaaggga tgggaggaga atcagatgag gaccatcatt ctgctgacga tatcgaatat
     3121 tttcatgacc ttcgcctggt acggccacct caagtacaag aattcgccgc tct
//
LOCUS       DAOQYN010000213         3145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_32748_length_3145_cov_3.370227, whole genome shotgun sequence.
ACCESSION   DAOQYN010000213 DAOQYN010000000
VERSION     DAOQYN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32748_length_3145_cov_3.370227"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="PKN80_07860"
     CDS             complement(<1..1733)
                     /locus_tag="PKN80_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNQ35961.1"
                     /translation="MVILTGLITRDDFFREELSRLLSDRCRLLNFTDETVRAPGNLEG
                     IDLFFLDLPVDRGMLELLGLLLSEKPELTVICFLDESDQRLAPEVSALGAYEVLSRNL
                     ADGQAAGSIRNALERSRLLAEKRTLERCPELTGQPPGTAGEPAAGRNLYQTAFPRFAG
                     TLARVFDAGEFWNSLAATLAETFGLSRVALFLEESGRFRFQAGTGWDPELSKQLDFDS
                     ANPLVKRLISERQLVTRWEIDQLPVPAAEAGAIRRELSLLQAWLCIPLFWRGRVNGFI
                     ALGNKVRGGGFTEAELELAVFLTSYLGLMAENVRLYHRISRSKREQEMVFRDLAAGVI
                     AADREGRIFTFNDRASEILRLPKAEVVGQAIQRTGSQLADLFWRTLRETDAEPEKKLV
                     YQPTATPLQVHASLLHDENERVEGVVIVLLDLTEKERLENKVRELERLNYWYQLAGSM
                     AHQIKNPLVSIKTFVQLFPEKYRDPEFRDNFFKIVNREVERLNEIIAELFEFSEPGAL
                     HLQPCPLNEILAEVIRRAETEGGPRISPPAENEEKAWLNADRDRLVKAFGCLVENARE
                     AVRKNKNGLIE"
     gene            complement(1752..>3145)
                     /locus_tag="PKN80_07865"
     CDS             complement(1752..>3145)
                     /locus_tag="PKN80_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ35962.1"
                     /translation="ASVLRSTTVYTLATLLLAVFYSLAVLVFNWIFFYFKPGNSFLPN
                     LLAAVIIAFIFAPVRQHSENLANRLFLRGKPTQPEKLLGEVTGIFTNRLDITRTLPES
                     LRLISDFLGTSRAAVYLREIGSENRFLLFDSFPGASDRLPAELSPDSPLLGKNWPKTV
                     LVKTELERLPVNPVNSRLLEALAATGLEVAVDLALGKERLGLLLLGEKNNGKLYTGQD
                     LNLLQAVTGQLAVFLKNTELFNQLQQAEKLMAVGTLAAGLAHEIRNPLVSIKTFIQLL
                     PEKFHDSEYRQAFSQIASQEVERINNLIQELLNFARPAPINKQPTDLVKIIERSLFLL
                     DPEFRKRAIDIEKRLPAEAFLMGDAEQLHRVFSNLLLNSIQAIDQSGRITVEIIQNPG
                     ENRLLVNFKDTGKGIPPENLPRLFDPFYSSRIGGTGLGLSIAHNIVKEHGGRIEVESQ
                     PGKGSTFRLDFQT"
BASE COUNT          567 a          913 c         1037 g          628 t
ORIGIN      
        1 atttcgatca ggccgttctt gtttttgcgg accgcttccc gggcgttctc caccaggcat
       61 ccgaaggcct tgaccaggcg gtcccggtcc gcgttcagcc aggccttttc ctcgttttcg
      121 gccggcgggg agatcctggg accgccctcg gtttccgccc ggcggattac ctcggccagg
      181 atttcattca gcgggcaggg ctgcaggtgc agcgcgcccg gttccgaaaa ctcgaagagt
      241 tcggcgatga tctcgttcag gcgttccact tcccggttaa cgatcttgaa gaaattgtcc
      301 cggaattccg ggtcccggta tttttcgggg aagagctgaa cgaaggtctt gatggaaacc
      361 agggggtttt tgatctggtg cgccatgctg ccggccagct ggtaccagta attgagccgc
      421 tcgagttcac gcaccttgtt ttccagacgc tccttttcgg tcaggtcgag caggacgatg
      481 accactccct ccactctttc gttctcgtca tgcaggaggc tggcgtgcac ctggagcggg
      541 gtggcggtcg gctggtagac cagctttttc tcaggctccg catcagtttc ccggagggtg
      601 cgccagaaga ggtcggccag ctgggagccg gtccgctgga tagcctgacc gaccacctca
      661 gccttgggga ggcgcagtat ttcggaagcc cggtcgttga aagtgaagat ccggccttcc
      721 cgatcggcgg cgatcacgcc ggcggccagg tcacggaaga ccatctcctg ttcccttttc
      781 gacctggaga tccggtggta aaggcggacg ttttcagcca tcaggcccag gtaggaggtc
      841 agaaaaacgg ccagttccag ttccgcttcg gtgaacccgc cgccccggac cttgttgccg
      901 agcgcgataa aaccgttgac ccggccgcgc cagaagagcg ggatgcagag ccaggcctgg
      961 agcagggaca gttcccgccg gatcgccccg gcctccgccg ccgggaccgg gagctggtcg
     1021 atttcccagc gggtgaccag ctgccgttcg ctgatcagcc gcttgaccag cgggttggca
     1081 gaatcgaaat cgagctgttt cgaaagttcc gggtcccagc cggtgccggc ctggaaacga
     1141 aagcggcccg attcctcgag gaagagggcc acccgggaga ggccgaaggt ttcggcgagc
     1201 gtggcggcca gggaattcca gaactcaccg gcatcgaaga cccgggccag ggtcccggca
     1261 aaacgcggga aggccgtctg atagaggtta cggccggccg ccggttctcc ggccgttccc
     1321 ggcggttgac cggtgagttc cggacacctt tccagcgtcc gtttttcagc caggagccgg
     1381 ctgcgttcca gggcgttccg gatcgaaccg gccgcctgcc catcggcgag gttcctggag
     1441 aggacttcat aggccccgag cgccgaaact tccggggcca gccgctggtc cgattcgtcc
     1501 aggaaacaga tcacggtcag ttccggtttt tcggagagca ggaggccgag cagctccagc
     1561 attccgcggt caacgggcag gtccaggaag aagaggtcaa ttccctcgag gttgccgggc
     1621 gcccggaccg tctcgtcggt gaaattcagg aggcggcacc ggtcggacag gagccgggat
     1681 aattcctccc ggaaaaagtc atcccgggtt ataaggccgg tcaatatcac cattcctcct
     1741 ctgattcgcg gttaagtctg aaaatcgagc cggaaggtgc ttcccttgcc cggctggctc
     1801 tccacctcga tccggccgcc atgctccttg acgatgttgt gggcgatgga gagtcccagg
     1861 ccggttcccc cgatgcggct gctgtagaaa ggatcgaaga gccggggcag attttccggc
     1921 ggaatgccct tgccggtgtc cttgaagttt accagcagcc ggttctcccc ggggttttga
     1981 atgatttcga ccgtgatccg gccggattgg tcgatggcct ggatgctgtt gagcagaagg
     2041 ttggaaaaga cccggtgcag ctgttccgcg tcacccatca ggaaggcctc ggccggcagc
     2101 cgtttctcga tgtcgatcgc ccgcttccga aactccgggt ccagcaggaa gaggctccgc
     2161 tcgatgattt tcaccaggtc ggtcggttgc ttgttgatcg gcgccggccg ggcgaagtta
     2221 agcagttcct ggatcaggtt gttgatccgt tccacttcct gcgaggcgat ctgggagaag
     2281 gcctgacggt attcggagtc gtggaatttt tccgggagca gctggatgaa ggttttgatc
     2341 gagaccagcg gattccgtat ttcatgggcc agcccggcgg ccagggtgcc gaccgccatc
     2401 agcttttcgg cctgctgaag ctggttgaag agctcggtgt ttttgaggaa aacggccagc
     2461 tgtccggtga ccgcctggag caggttgaga tcctggccgg tgtataattt gccgttgttc
     2521 ttttcaccca gcagcagcag gcccagccgt tccttgccaa gcgccaggtc aaccgccact
     2581 tccagcccgg tggcggccag ggcctcgagc agccttgaat ttaccgggtt gaccggaaga
     2641 cgctccagct ccgtcttgac caggacggtc ttcggccagt ttttgcccag caggggtgaa
     2701 tcgggggaga gttccgccgg cagccggtcc gaagctccgg gaaaggaatc gaagagcaga
     2761 aaccggttct cggagccgat ttcccgcagg tagaccgccg cccgggaagt tccgaggaag
     2821 tcgcttatca gccgcaggga ttccgggagg gtccgggtaa tgtcgagccg gttggtgaag
     2881 atgccggtca cctcgcccag cagtttttcc ggctgggttg gcttgccgcg cagaaaaagc
     2941 cggttggcca ggttttcgct gtgctggcga acgggtgcga agatgaaggc gatgatcacc
     3001 gccgccagca ggttgggcag gaaggagttg ccgggcttga agtagaagaa gatccagttg
     3061 aagaccagga ccgccaggga gtaaaagacg gccagcagca gcgtggccag ggtgtaaacg
     3121 gtggtgctgc ggaggaccga ggcca
//
LOCUS       DAOQYN010000214         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_33252_length_3113_cov_3.082734, whole genome shotgun sequence.
ACCESSION   DAOQYN010000214 DAOQYN010000000
VERSION     DAOQYN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33252_length_3113_cov_3.082734"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PKN80_07870"
     CDS             complement(<1..840)
                     /locus_tag="PKN80_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35963.1"
                     /translation="MLTRRENYTRILTRGNGDRLPFVPNFDYWYRVNRANGRIPEAYA
                     GLSRNDLVRAVGATLWVRTNVLEPVLDPTVEVTERDDGRRRRTEYRTPVGTVSTLHEY
                     ADDSARTCYLMEHRIKRAEDIPVVKYLFEHTSLKADFTSFLESEAEVGEDGISLVGLP
                     FCFPYIHFAKQEAGYQAGIYLYADYPELVEELMEVIARVNLAGAELLASGPALVVQQG
                     DNMDEWTTPPNLFKRLAVPYFRRVAEILHRAGKIYQGHWCGRTEHLLPLVPGSGLDVI
                     EAVS"
     gene            complement(884..2011)
                     /locus_tag="PKN80_07875"
     CDS             complement(884..2011)
                     /locus_tag="PKN80_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35964.1"
                     /translation="MNARERWIEALTFGKPDRLPLLPGGPRESTLKRWHQEGLPADRP
                     WSEVLMETLGIQPETTKPQVNLGVNFDLMPAFEEKILEHRDGHYLMQTIRGEIVEVSD
                     RYDFTYLRSAKDFVTRKYHRHPVENRADWDAMKKRFNPDTPGRFPEDFEVRCRQLADR
                     DYVLRIWFNGPFWQLRNWCGFENLCLFMAEDPELVEEMALFWRDFIMTILDRIFARVA
                     PDHFGFSEDMAYKAKSMISPAMARRFLMPCWQTWSAAARRSGCRIIDVDSDGYIGELI
                     PLWIESGINSCQPIEVAAGNDLPAYRKQFGRSMAYHGGIDKRAIAKGGAVIEAELQRV
                     IPPMLVDGGYVPSCDHGVPPDISWADFIHYSRELAKLTGWL"
     gene            complement(2186..2896)
                     /locus_tag="PKN80_07880"
     CDS             complement(2186..2896)
                     /locus_tag="PKN80_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35965.1"
                     /translation="MNLSLFVPLLVAHLLAVNLAAAGPLAGGALLAVMKHFEATLPLG
                     RYEILFLVEGTLRLLSLGGLPLRFREARETDWHSDYRAYLEQFPSGEHAAAARERLAW
                     LAGRKAVPEIVVSAVVDADDGDVNAPGPRWRWTTQLYERGGQIGYRVSGQGFILDPRG
                     GLWMGENDARIPRPRIRVEAGGSARDTYWISSRPHLLCNGVAIFTWEGEDAGGHPIRL
                     EERIQLRHLNCPGPTPAR"
BASE COUNT          534 a          968 c         1010 g          601 t
ORIGIN      
        1 cgagaccgcc tcgatcacat ccaggccgct gccgggcacc agtggcagca ggtgttcggt
       61 tcggccgcac cagtggccct ggtagatctt gccggcccgg tgcaggattt cggccacccg
      121 gcggaagtaa gggacggcca ggcgcttgaa gaggttcggc ggcgtggtcc actcgtccat
      181 attgtcgccc tgctgaacga ccagcgccgg tccgcttgcc agcaattcgg ccccggccag
      241 attaacccgc gcgatcacct ccatcagttc ctcgaccagc tccgggtaat cggcgtagag
      301 atagatgccg gcctggtaac cggcttcctg cttggcaaaa tgtatgtaag ggaagcagaa
      361 tggaagtccg accagagaga tgccgtcctc gcccacttcc gcttcgctct cgaggaaaga
      421 agtgaaatcg gccttgaggc tggtatgctc gaagaggtat ttgaccaccg ggatatcctc
      481 ggcccgcttg atccggtgct ccatcaggta acaggtgcgt gccgagtcgt cggcgtactc
      541 gtgcagggtg ctgacggttc cgaccggcgt ccggtattcg gtccggcgcc gccgcccgtc
      601 atcccgctcg gtcacctcga ccgtcgggtc cagcaccggc tccaggacgt tggtgcggac
      661 ccagagggtg gcaccgaccg cccggaccag gtcgttccgg ctcagcccgg cgtaggcttc
      721 cggtatccgg ccgttggccc ggttgacccg gtaccagtag tcgaaattag gcacgaaggg
      781 caggcggtcg ccgttgcccc tggtcagaat acgggtataa ttttcccgcc tggtaagcat
      841 gggttttctg cctcgcggtt tttcaggccg gccgcggcct ttttcagagc cagccggtca
      901 gtttcgccag ttcccggctg taatggatga agtcggccca ggagatgtcg ggcggtacgc
      961 cgtggtcaca cgagggaacg tagccgccgt caaccagcat gggcggtatc acgcgctgga
     1021 gttcggcctc gatcaccgct ccgcccttgg cgatggcccg cttgtcgatg ccgccgtggt
     1081 aggccatgct gcggccgaac tgcttccggt aggcgggcag gtcattgccg gcggccactt
     1141 cgatcggctg gcaggagtta atgcccgact cgatccagag cggaatcagt tcgccgatgt
     1201 agccgtcgga gtcgacgtcg atgatccggc agccgctccg gcgggccgcc gccgaccagg
     1261 tctgccagca gggcatcagg aaacggcggg ccatggcggg tgagatcatg ctcttggcct
     1321 tataggccat gtcctcggag aaaccgaaat gatccggggc gacgcgggca aagatccggt
     1381 ccaggatcgt catgatgaaa tcgcgccaga agagggccat ctcctcgacc agttccgggt
     1441 cctcggccat gaagaggcag agattttcaa agccgcacca gttgcgcagc tgccagaagg
     1501 ggccgttgaa ccagatgcgc aaaacgtaat cccggtcggc cagctgccgg cagcggacct
     1561 cgaagtcctc gggaaaacgg ccgggcgtgt cggggttgaa gcgttttttc atggcatccc
     1621 agtcggcccg gttttcgacc ggatggcggt ggtacttgcg ggttacgaag tccttggcgc
     1681 tgcgcaggta ggtaaaatcg taccggtcgg agacctcgac gatctcgccc cggatggtct
     1741 gcatcaggta atggccgtcg cggtgctcaa gaatcttctc ttcgaaggcc ggcatcaggt
     1801 cgaagttgac ccccaggttg acctgcggct tggttgtctc cggttggatc cccagcgttt
     1861 ccatcaggac ttcagaccag gggcggtcgg ccgggaggcc ctcctggtgc cagcgcttga
     1921 gcgtggattc gcgggggccg cccggcagca gcggtaaccg gtccggtttg ccaaaggtga
     1981 gcgcctcgat ccatctttct cttgcgttca ttgttttccc cccggttttt tccgttgatg
     2041 gtttaaatag cggcccggcc ggctgatcca gccgtgatta tttatcctca attttaacat
     2101 tttaagtttt ttttgcctat ttatcacggc gaacggggaa ggattggacg ttggatccgg
     2161 ggcgaagctg gaggccggcc gggtttcagc gggccggggt tgggccggga cagttaaggt
     2221 ggcgaagctg gattctttct tcgaggcgga tggggtgacc gccggcatcc tctccctccc
     2281 aggtgaaaat ggcgaccccg ttgcacagca ggtgggggcg cgaggagatc cagtaggtgt
     2341 cccgggcgct gccgccggcc tccaccctga ttctcggtct tggaatccgg gcgtcattct
     2401 ctcccatcca gaggccgccg cggggatcga ggatgaatcc ctgtccgctg acccggtaac
     2461 cgatctgccc gccgcgttcg tagagttggg ttgtccacct ccagcggggt cctggggcgt
     2521 tgacgtctcc gtcgtcggcg tcgaccacgg ccgaaacgac gatctccggg accgccttcc
     2581 gcccggccag ccaggccagc ctttcccggg ccgcggccgc gtgttctccg gacgggaatt
     2641 gttccaggta ggcccggtaa tccgagtgcc agtcggtctc ccgcgcctcc cggaagcgga
     2701 gcggcaggcc gcccaggctc aggagccgca gggtcccctc gacgagaaaa aggatctcgt
     2761 agcgtcctaa gggaagcgtc gcctcgaaat gcttcatcac cgccagcaac gctccgcccg
     2821 ccagcggtcc cgccgccgcc aggttcacgg ccagcaaatg agccaccagc agcggtacga
     2881 acaacgacaa gttcacaacg ccgatccgtg caagagtgag aacgtcgaaa gccgggcggc
     2941 gggcgatcgt tgccacggcc cacgtttgcg gcgattatag cgcaagatgg ccagatcggg
     3001 catggatcgc aatcgttcgc caccctaaag aagtacgcca ttgcggccgt cccgccagga
     3061 attcgctggc ggtgcatcgc gccgtgccct agccccagcc attgtttgcg gcc
//
LOCUS       DAOQYN010000215         3023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_34709_length_3023_cov_4.329515, whole genome shotgun sequence.
ACCESSION   DAOQYN010000215 DAOQYN010000000
VERSION     DAOQYN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34709_length_3023_cov_4.329515"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PKN80_07885"
     CDS             <1..883
                     /locus_tag="PKN80_07885"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNQ35966.1"
                     /translation="LMNSGPNRVSPFLIPMLIPDIAAGRIAMLFNCQGPNFAAVSACA
                     SGAHALGISLRSIQYGEVDLVISGGTESCITPLGLAGFCSMKALSERNDEPARASRPF
                     DRGRDGFVMAEGAGILVLEELEHARRRGARIYAEFSGYGASADAYHITAPDPEGKGMM
                     LAMKMALNDAGLAPEQIDYINAHGTSTQLNDQVETRSIKNVFGTAAGRVPVSSTKSMT
                     GHMLGAAGAVELAAGALVITRGVLPPTINQEEPDPDCDLDYVPNQAREVPAVRAILSN
                     SFGFGGHNASLILSRME"
     gene            889..2049
                     /locus_tag="PKN80_07890"
     CDS             889..2049
                     /locus_tag="PKN80_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNQ35967.1"
                     /translation="MDYLLTEEQKMIRDLCRQIGEEKIKPVRAHYDETEEFPWEVVKV
                     FAESDLMGIFIPVEYGGMGFGVTELSVAIEELSRYCGGIALALAGTGLGTFPILLFGS
                     EEQKKKYLPRIARGEALAAFALTEANAGSDAAGIETTAARDGDHFVLNGTKQWITNGG
                     EAEIYTVVAMTNKSRGARGASAFIVEKGTPGFDFGKKEKKLGIRASATRELVFQDCRV
                     PKENLLGREGTGFLVAMRTFDASRPGVAAQALGIAQGALDEALQYVRTRVQFGKPIGS
                     FQAVQHMLADMATQIEAARALIYSTSRMYDAGVGDTSKESAMSKLFASDVAMRVTTDV
                     VQIFAGYGYMKDYPVEKMMRDAKITQIYEGTNQIQRNVIGLELIKEAARRHK"
     gene            2090..2968
                     /locus_tag="PKN80_07895"
     CDS             2090..2968
                     /locus_tag="PKN80_07895"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNQ35968.1"
                     /translation="MRLDKYLSETTGQSRQHIQRLIASGRVLVNGRPARKNQPAGPDD
                     RIEITWPEAEADGLAAENIPLAILYQDADLAVIDKPAGLVTHPYGRVISGTLVNALLY
                     HLSGLSGIGGVRRPGIVHRLDRETSGLMVVAKNDFSHRFLSNEFRSRRVHKKYLAFVK
                     GVIPWKEQEVCLHLNRKRGQGLKIGLDLRGGRESQTRLAVSRRFPRASLVEVFPRTGR
                     THQIRVVLSFLGFPIIGDRTYGRPQPIDRLIGRQALHAAELSFTHPRSGDKQVFHSPL
                     PEDLAKLAADLTGWNT"
BASE COUNT          586 a          951 c          954 g          532 t
ORIGIN      
        1 cctgatgaat tccggcccga accgggtttc ccctttcctt atcccgatgc tgatcccgga
       61 catcgcggcc ggccggatcg ccatgctctt caactgtcag gggccaaact tcgcggccgt
      121 ttccgcctgc gcctcgggag cgcacgcgct cgggatatcc ctgcgcagca tccagtacgg
      181 cgaggtcgac ctggttatct ccggcgggac cgagtcctgc atcacccccc tgggcctggc
      241 cggtttctgc agcatgaagg ccctctcgga gcgcaatgat gaaccggccc gggccagccg
      301 tccctttgac cgcggccgcg acggtttcgt gatggccgag ggcgccggaa tcctggtgct
      361 cgaggaactg gaacacgccc ggcgccgtgg cgccaggatc tacgccgaat tttccggtta
      421 cggggccagc gccgacgcct accacatcac cgcgcccgat ccggagggga aaggcatgat
      481 gctggccatg aagatggccc tgaacgatgc cggcctggct ccggagcaaa ttgattatat
      541 aaacgcgcac ggaacctcga cccagcttaa tgatcaggtg gagacgcggt ccatcaagaa
      601 cgtcttcgga accgccgccg gacgggtgcc ggtcagttcc accaagtcaa tgaccggcca
      661 catgctcggg gcggccggcg cggtcgaatt ggccgccggc gccctggtga tcacccgggg
      721 cgtgttgccg cccaccatca accaggaaga gccggatccc gattgcgatc tcgattacgt
      781 tcccaaccag gcccgggaag tgccggcggt ccgggccatt ctttccaact cattcggatt
      841 cggcgggcac aacgcctccc tgatcctgag ccggatggag tgaccgatat ggactatcta
      901 ttgaccgaag aacagaagat gatccgggat ctctgccggc agatcgggga ggagaagatc
      961 aagccggtgc gggcccatta cgacgagacc gaagagtttc cctgggaagt ggtcaaggtc
     1021 ttcgccgaga gcgacctgat gggaatcttc atcccggttg agtacggcgg catgggtttc
     1081 ggggtgaccg aactctcagt ggccatcgag gagctttccc gctactgcgg agggatcgcc
     1141 ctggccctgg ccggaaccgg gctgggcacc ttccccatcc tgctcttcgg gagcgaggag
     1201 cagaaaaaga agtacctgcc ccggatagcc aggggggagg cactggcggc ctttgccctg
     1261 accgaggcca acgccggcag cgacgcggcc ggtattgaga cgaccgccgc ccgggacggg
     1321 gatcattttg ttctgaacgg caccaagcag tggatcacca acggcggcga agccgagatt
     1381 tacacggtgg tcgccatgac caacaagtcg cgcggcgccc ggggcgccag cgccttcata
     1441 gtggaaaaag gaacgcccgg tttcgacttt ggcaagaagg agaagaagct gggtatccgg
     1501 gcctcggcca cccgggagct ggtcttccag gattgccggg tgcccaagga gaacctgctg
     1561 ggccgggagg gcaccggttt cctggtggcc atgcggacct ttgacgcttc ccgtcccggc
     1621 gtggcggccc aggccctggg catcgcccag ggggcgttgg acgaggccct gcagtatgtg
     1681 cgcacccgag tccagttcgg caagccgatc ggaagcttcc aggccgtcca gcacatgctg
     1741 gccgacatgg cgactcagat cgaggccgcc cgagccctga tttactccac cagccgcatg
     1801 tacgacgccg gcgtcggcga caccagcaag gaatcggcga tgagcaagct tttcgcttcc
     1861 gatgtcgcga tgcgggttac caccgatgtc gtccagatct ttgccggtta cggctacatg
     1921 aaggattacc cggtggaaaa gatgatgcgg gatgccaaga tcacccagat ctacgagggt
     1981 accaatcaga tccagcgcaa cgtcatcggg ctggaactga tcaaggaagc cgcccggcgc
     2041 cataagtgac ggcgttccgg gcgtgctgtt ttccggccac cccatccgcg tgagactgga
     2101 caaatacctc agcgagacca ccggccaatc gcgccagcat atccagcggc tgatcgcttc
     2161 cggccgcgtg ctggtcaacg gaagaccggc gcgcaagaat cagccggccg ggcctgacga
     2221 ccggatcgag atcacctggc cggaggccga ggccgacgga ctggccgcgg aaaacatacc
     2281 gctggcgatc ctttaccagg atgcggacct ggccgttata gacaagccgg ccggcctggt
     2341 aacccatccc tatggacgcg tcatttccgg gacgctcgtc aacgctctgc tttaccacct
     2401 ttccggattg tccgggatcg gcggagtgcg ccggcccggt atcgtgcacc gtctggaccg
     2461 ggagaccagc ggactgatgg tggtggccaa gaatgatttc agccaccggt tcctgagcaa
     2521 tgagtttcgg agccgccggg tgcacaagaa atacctggcc ttcgtcaagg gcgtgatccc
     2581 ctggaaggaa caggaggttt gcctgcacct gaaccggaaa cgcggccagg gattgaagat
     2641 cggcctcgat ctccggggcg gccgcgaaag tcagacaagg ctggcggtaa gccgccgctt
     2701 cccccgggcc agcctggtgg aggtttttcc gcgcaccgga aggacccacc agatcagggt
     2761 ggtgctaagt ttcctgggtt tcccgatcat cggagaccgt acttacgggc gcccccagcc
     2821 gattgaccgc ctgatcggcc gccaggccct gcacgcggcc gaactttcat tcacccatcc
     2881 ccgatccggc gacaaacagg tttttcattc acccctgccg gaagatctgg ccaagctggc
     2941 ggctgacttg accggatgga atacttgagt ataatatcat tcttgtaagt ttccttttcg
     3001 aagggccaaa acagcgtttt tcc
//
LOCUS       DAOQYN010000216         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_35897_length_2950_cov_4.495682, whole genome shotgun sequence.
ACCESSION   DAOQYN010000216 DAOQYN010000000
VERSION     DAOQYN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35897_length_2950_cov_4.495682"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PKN80_07900"
     CDS             complement(<1..681)
                     /locus_tag="PKN80_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ35969.1"
                     /translation="MGSFLNRHLDFKRHNEEVDRVWAAFRRGEPYRVPVTVAGSIRNL
                     FGNPEINRTGYTFEDFFNDPKAHLECQLAYRKWVNYNLLCDAKLGPPEAGWPVTVDFQ
                     NSYEAMWFGCPLRCDGNQVPDTQPILQAEKKRLYDLEPPDPLRGNRLGRAMDFFEYLQ
                     AECPKREFEGRPVRPPESIPGEGTDGPFSIAYKLRGAAEACLDICEDPQYFHDLMNFV
                     TENSIRRMK"
     gene            complement(685..1605)
                     /locus_tag="PKN80_07905"
     CDS             complement(685..1605)
                     /locus_tag="PKN80_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNQ35970.1"
                     /translation="MADERPIPVILDTDIGGDIDDTWALAMMLRSPELDVRLIVTDTG
                     DTTYRARIVAKLLEIAGRTDIPIGLGLPQASDGPRERQAAWVAGYDLRRYSGPVLADG
                     VGALIETIRCSPRPVTLVCIGPVPNIGAALRREPRIAENCRFVGMLGCFRRSHGGAPR
                     VIAEYNVVQDVPASQAVFAAPWPMTITPLDTCGLVRLSGDRYRAVCDCPDPLVRAVVE
                     NYRVWLASRGNYSYASETESSFLYDTVAVHLAFNTQFLAMEQLGARVDERGFTLEAGG
                     GRPVLAALDWTDLEGYENFLVDRLTNRGKS"
     gene            complement(1783..>2950)
                     /locus_tag="PKN80_07910"
     CDS             complement(1783..>2950)
                     /locus_tag="PKN80_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNQ35971.1"
                     /translation="LTNGDHDEIAFKIDAGRFALTPREYLAMGERRRRESAAAAEFLG
                     LEKGDLIFLGYPDFGTMHIWEQHWGPAPAFSNDLTRASAVPYPDNFSYRAPYKGESIV
                     ADLLRILKEYRPTAIFVTMPADFNADHQAAWCFLSLAVLEAGFQTRPPDVFLFLVHLG
                     GWPRPLHYHPNLPLLPPARLTGSELSWFKLPLTPEETGRKYRALRFFASVMKGRAYLW
                     SAFARQNELFATLPEFRMRKVFPSAWEKALEVENLEAGDRKAVRKEEADLEGVSYLKV
                     DDHLLVRMLLKRKLAIETGLSLYLFGYRRDVTFNSMPKIRLAASILKTVPVYDGSRKI
                     GDLPVKLAGDGTELLLSVPLAMLGRPEAIFTSLHFRRADLSLDSTAWHLLRLQD"
BASE COUNT          552 a          940 c          938 g          520 t
ORIGIN      
        1 cttcatccga cggatgctgt tctcggtgac gaagttcatc aggtcgtgaa aatactgcgg
       61 gtcctcgcaa atgtcgaggc aggcctccgc ggccccccgc agtttgtagg cgatcgaaaa
      121 gggtccgtcg gtcccctcgc ccgggatgct ttccggcggc cgcaccggcc gtccctcgaa
      181 ttcccgcttc gggcactccg cctgcaggta ctcgaaaaaa tccatcgccc ggcccagccg
      241 gttgccccgc aggggatcgg gcggctccag gtcgtacaac cgtttcttct ccgcctgcag
      301 gatcggctgg gtatcgggca cctggttgcc gtcgcagcgc agcggacagc cgaaccacat
      361 cgcctcgtag gagttctgaa aatcgaccgt gaccggccag cccgcttcgg gcggccccag
      421 cttcgcgtcg cagagcaggt tgtagttcac ccatttccgg taggccagct ggcactcgag
      481 gtgggccttc ggatcgttga agaaatcctc gaaggtgtaa ccggtcctat tgatctccgg
      541 attgccgaag aggttccgga tgctgccggc gaccgtaacc ggaacccggt agggctcgcc
      601 ccggcgaaag gccgcccaga cccggtcaac ctcttcgtta tgccgcttga aatccaggtg
      661 ccgatttaaa aaagaaccca tttttcaact ctttccccgg ttggtcagcc ggtcaaccag
      721 gaaattttca tatccctcga ggtcggtcca gtccagcgcc gccaggaccg gccggccacc
      781 gccagcctcc agggtgaagc cgcgctcgtc aacccgggcg ccgagctgct ccatggccag
      841 gaattgcgtg ttgaaggcca ggtggacggc cacggtatcg taaaggaaac tgctttccgt
      901 ctccgaagcg tacgaataat tcccccggga agccagccag acccggtagt tctcgaccac
      961 tgcgcgcacc agcggatccg ggcagtcaca gacggcccgg tagcggtcgc cggacaatcg
     1021 gaccaggccg caggtatcca gcggcgtgat ggtcatcggc cagggggccg cgaagaccgc
     1081 ctggctggcc ggtacgtcct ggacaacgtt atactcggcg atgaccctgg gcgcgccgcc
     1141 gtggctccgg cggaagcagc ccagcatgcc gacgaaacgg cagttttcgg caatccgcgg
     1201 ttcgcgccgc agcgcggcgc cgatgttcgg caccgggccg atgcagacca gcgtcaccgg
     1261 ccggggggag caacggatgg tttcgatcag cgcaccgacg ccgtcggcaa gcaccgggcc
     1321 ggagtagcgg cgcagatcgt aaccggccac ccaggcggcc tgccgttcgc gcggaccgtc
     1381 gctggcctgg ggcaggccga ggccgatcgg gatatcggtc cggccggcta tttccagcag
     1441 tttcgccacg atccgggcgc ggtaggtggt atcgccggtg tcggtgacga tcagcctgac
     1501 gtccagttcc ggagagcgca gcatcatcgc cagcgcccag gtgtcgtcaa tgtcgccgcc
     1561 gatgtccgtg tccaggatca ccgggatcgg cctttcatcc gccatggccc atctccctca
     1621 ccgggcattg atcgtctccg ggaaaaatga attttgataa tttaatagtc gtattgtacc
     1681 ccagtccggg cgccggaaac aatgacggac cgcagacgaa acggagtagc ctgttcagat
     1741 ccagaccttg cggtcgaggg tgcggccttc agcttctctt ccttaatcct gaagacgcag
     1801 gagatgccag gcggtcgagt cgagggagag gtcggcgcgg cggaagtgca gggaggtgaa
     1861 gatcgcttcc ggccggccca gcattgcaag gggcacgctg agcagcagct cggttccatc
     1921 accggccagt tttaccggca ggtcgcctat cttccggctt ccatcataga ccggaacggt
     1981 cttcaggatt gaggccgcca gccggatttt gggcatcgaa ttgaaagtaa cgtcccggcg
     2041 gtacccgaag aggtaaagcg aaagcccggt ctcgatcgcc agtttccgtt ttaaaagcat
     2101 ccggaccagg agatggtcgt caaccttcag gtaggagacc ccttccaggt cggcctcctc
     2161 tttccggacc gccttccgat cgccggcctc gaggttctcc acctccaggg ccttctccca
     2221 ggccgacgga aacaccttgc gcatcctgaa ttccggcagg gtggcaaaga gttcattctg
     2281 ccgggcaaag gcgctccaga gataggcccg ccccttcatg acgctcgcga agaagcgtaa
     2341 cgcccggtac ttccggccgg tctcctccgg cgtcaacgga agtttgaacc agctcaattc
     2401 gcttccggtc agccgcgccg gcggcaggag cggcaggttc ggatgatagt ggaggggccg
     2461 gggccagccg cccagatgca ccaggaaaag aaagacgtcg ggaggcctgg tttggaaacc
     2521 ggcctccagg acggccagag agaggaagca ccaggcggcc tggtgatcgg cgttgaaatc
     2581 ggccggcatg gtgacaaaaa tcgcggtcgg ccgatactcc ttcaggatcc ggagcaggtc
     2641 ggcgacaatg ctctctccct tgtaaggcgc ccggtaggaa aagttgtccg ggtagggaac
     2701 cgcgctggcc cgggtgagat cgttggagaa ggccggcgcc gggccccaat gctgttccca
     2761 gatatgcatg gtcccgaaat cgggataccc caggaagatc aggtcaccct tctccagccc
     2821 caggaattcg gcggcggcgg cggactcccg gcgccgccgt tcgcccatcg ccaggtactc
     2881 gcgcggcgtg agcgcaaacc ggccggcatc gatcttgaag gcgatctcgt cgtggtcgcc
     2941 gttggtcagg
//
LOCUS       DAOQYN010000217         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_36251_length_2932_cov_4.066736, whole genome shotgun sequence.
ACCESSION   DAOQYN010000217 DAOQYN010000000
VERSION     DAOQYN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36251_length_2932_cov_4.066736"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1606)
                     /locus_tag="PKN80_07915"
     CDS             complement(<1..1606)
                     /locus_tag="PKN80_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNQ35972.1"
                     /translation="MEEKIIVRGAREHNLQNIDLELPRNRLIVVTGISGSGKSSLAFD
                     TLYAEGQRRYIESLSAYARQFLEQMQKPDVDHIWGLPPAIAIEQRRAGGNPRSTVATI
                     TEIYDYLRLLFSRVGTPHCPKCGRVISRQTVQDITDRILELPAATSVSILAPMVRGRK
                     GEYREIFAQVARAGFLKVRVDGRFHEVSEEIKLSRYRNHTIEVLVDTLEVSPAEKARV
                     TESVETGLKLGQGLLTVLVGSETLLFSERYACSACGFNFEELTPRMFSFNSPYGACPE
                     CKGLGTQIVFDPDLVVPDKRKTLREGAVKPWQEAGGRGIFLYYRWLLRSLAKNLGFSL
                     DHRYADLSREQRQALLYGSQEHEFEGVIPNLKRRYLETESEYRREELSRYMRELPCSA
                     CQGARLKPESLGVTVNGRSIWDVVRLSVEKARDFFAALDLDERQAIISSQVIKEIRSR
                     LQFLISVGLEYLTLDRVGHTLSGGESERIRLATQVGSGLVGVLYILDEPSIGLHQRDN
                     SKLLKTLQDLRDLGNTVVVIEHDEETI"
     gene            complement(1709..>2932)
                     /gene="hisD"
                     /locus_tag="PKN80_07920"
     CDS             complement(1709..>2932)
                     /gene="hisD"
                     /locus_tag="PKN80_07920"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNQ35973.1"
                     /translation="ERLESILAERRRRFDAAESAAAGIIEKVRAAGDEALAEFTLRFD
                     GVALKRFQVGPAETMAARRAFLTGEWLARLQRVTDRLVDFARRELPAGFTAGGRGERV
                     ERVFNPISPVGIYIPAGTAPLVSTVLMTVVPARVAGVREIVLVTPPDRRGRANPAVVA
                     TAAWLGVERIFKIGGAQAVAALAFGTRQVPRVAKIVGPGNEYVAAAKRLLYGRVDIDC
                     PAGPSEVVVCADRTAPVEFVKAELAAQAEHRQGLAVLVTADRSFARKMLAAGGPGVMR
                     LVTPGRLVDELNGLAPEHLVLIGRSAAGLARRIDRAGAVFVGAYSPVALGDYLAGPSH
                     VLPTGGAAAAFSGLSVLTFLRSSSRIAWSRSGLKAWARDLEALAELEGLPAHRLSVNR
                     RFSPVPARGGRAAEK"
BASE COUNT          488 a          988 c          998 g          458 t
ORIGIN      
        1 ggatggtctc ctcgtcgtgc tcgatcacca ccacggtgtt gcccaggtcg cgcaggtcct
       61 ggagggtctt gagcagcttg ctgttatccc gctggtggag tccgatgctc ggctcgtcca
      121 ggatgtagag gacgcccacc aggccgctgc ccacctgggt ggccaggcgg atgcgctcgc
      181 tctcgccgcc ggaaagggta tggccgaccc ggtccagggt caggtactcc aggcccaccg
      241 aaatcaggaa ctggaggcgg ctccggatct ccttgatcac ctggctgctg atgatcgcct
      301 ggcgctcgtc caggtcgagc gccgcaaaga aatcccgggc cttctccacc gacagccgga
      361 ccacgtccca gatgctccgg ccgttcacgg taacccccag cgactccggc ttgagccggg
      421 cgccctggca ggccgaacag ggcagttcgc gcatgtaacg gctcaactcc tcgcggcggt
      481 actcgctctc ggtctccagg tagcgccgct tgaggttggg aattacgccc tcgaactcgt
      541 gctcctggct gccgtagaga agcgcctgcc gctgctcccg gctgaggtcc gcgtaacggt
      601 ggtcgaggct gaagccgagg ttcttggcca ggctgcgcaa taaccagcgg tagtataaaa
      661 agatgccgcg gccgccggcc tcctgccagg gcttgaccgc gccctcccgc agggtcttgc
      721 gcttgtccgg caccaccagg tcgggatcga agacgatctg ggtccccagg cccttgcact
      781 ccgggcaggc cccatacgga ctgttgaagg agaacatgcg cggggtcagt tcctcgaagt
      841 tgaagccgca ggcggaacag gcgtaacgct cgctgaagag cagtgtttcg gaaccgacca
      901 ggaccgtgag caagccctgg ccgagtttca ggccggtctc gaccgattcg gtcacccgcg
      961 ccttctcggc cgggctcacc tccagggtat cgaccaggac ctcgatggtg tggttccggt
     1021 aacggctcag cttgatctcc tcggatacct cgtggaagcg gccgtcaacc cgtactttca
     1081 ggaacccggc ccgggccacc tgggcaaaaa tttcccggta ctcgcccttg cggccgcgca
     1141 ccatcggcgc cagaatcgag accgacgtgg cggcgggtag ttccaggatc cggtcggtga
     1201 tgtcctggac ggtctggcgg ctgatgaccc ggccgcactt cgggcaatgg ggcgtcccga
     1261 cccggctgaa aaggaggcgc aggtaatcgt agatctcggt aatggtcgcc accgtcgaac
     1321 gggggttgcc gccggctcgg cgctgctcga tggcgatggc cgggggcagg ccccagatgt
     1381 ggtccacgtc cggtttctgc atctgttcca ggaactggcg ggcgtaggcc gacaggcttt
     1441 ctatgtaacg gcgctgcccc tcggcgtaga gggtatcgaa agccagcgac gacttgccgc
     1501 tgccggagat gccggtgacc acgataaggc ggttgcgggg cagttccagg tcgatgttct
     1561 gaaggttatg ctcccgggcg ccccggacaa ttatcttttc ttccataaga ttgcacggac
     1621 ggtatgggtt atttccatat ttcaaaaaaa gccgccggag caaccggcgg cgagaaaagg
     1681 ccggccccgg ccccgagcaa atccgccgtt atttttcagc ggcccggccg ccgcgggccg
     1741 gaaccggcga aaaacggcgg ttcacgctga gacggtgagc cggcagccct tccagttcgg
     1801 ccagcgcctc caggtcgcgg gcccaggcct tgagtccgga ccggctccag gcaatccggg
     1861 acgaggagcg gaggaaagtc agaaccgaga gtccggagaa ggcggccgcg gccccgccgg
     1921 tcggcaggac atggctgggt ccggccaggt aatccccgag cgcaaccggg ctgtaggccc
     1981 cgacaaaaac ggcgccggcc cggtcgatcc ggcgggcgag cccggccgcc gaccggccga
     2041 tgagaaccag gtgctcgggg gccaggccgt tgagctcgtc caccagccgg ccgggcgtca
     2101 ccagccgcat gaccccgggg ccgccggcgg ccagcatctt ccgggcgaag gaccggtcgg
     2161 cggtaaccag caccgccagc ccctggcggt gttcggcctg ggcggccagt tcggccttca
     2221 cgaattcgac cggcgcggtc cgatcggcgc aaacgacgac ctcgctcggg ccggccgggc
     2281 agtcgatatc gacccggccg taaaggaggc gcttggcggc cgccacgtac tcgttgcccg
     2341 gcccgacgat cttggccacc cggggcacct gccgggtgcc gaaggccagg gcggcgacgg
     2401 cctgggcgcc tccgatcttg aagatccgtt ccacgccgag ccaggcagcg gtcgccacca
     2461 cggccgggtt ggcccggccc cggcggtccg ggggcgtgac caggacgatc tcccggacgc
     2521 cggccacccg ggccggcacg accgtcatca ggaccgtcga aaccagggga gcggtcccgg
     2581 ccgggatata aatgccgacc ggcgagatcg gattgaaaac gcgttccacc cgctcccccc
     2641 ggccgccggc ggtaaatccg gccggcagtt cccggcgggc gaagtcgacc agccggtcgg
     2701 tcacccgctg gagccgggcg agccattccc cggtgaggaa ggcgcgtcgg gcggccattg
     2761 tttcagccgg accgacctga aaacgcttca gcgccacgcc gtcgaaacgg agggtgaatt
     2821 cggccagggc ctcgtcgccg gcggcccgaa ccttttcgat gatcccggcg gctgcggact
     2881 cggccgcgtc aaaacggcgg cgccgttccg ccaggatgga ttccagccgt tc
//
LOCUS       DAOQYN010000218         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_36343_length_2928_cov_4.265924, whole genome shotgun sequence.
ACCESSION   DAOQYN010000218 DAOQYN010000000
VERSION     DAOQYN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36343_length_2928_cov_4.265924"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..2686)
                     /locus_tag="PKN80_07925"
     CDS             complement(29..2686)
                     /locus_tag="PKN80_07925"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNQ35974.1"
                     /translation="MEKQYNAREVEARWLQEYAGRPTFRAEAADPGEPFTIVIPPPNV
                     TGVLHIGHALNNTIQDLVIRRRRMAGRKALWIPGTDHAGIATQNVVERNLLEKGQRRD
                     DLGREKFLEEVWKWKESRGGTIIEQLKRLGCSCDWSRERFTMDPGLSRAVRTCFRRLY
                     QRGLIYRGDYIVNWCPRCGTALANEEVEHSPEESHLYHVRYPGLDGGPGVVVATTRPE
                     TLFGDTAVAVNPRDPRYRALVGKEVRLPILGRPIPVVAHPLVEKGFGTGAVKVTPHHD
                     PNDFRIGRDLGLPGLTVIDERGFLNAAAGPFAGQERFAAREGVVACLKEDGLLEKVED
                     YHHSVGRCYRCETVVEPYLSRQWFVRMKSLAAPALELARKGQPRFHPRRWQKVYQRWL
                     ENIEDWCISRQLWWGHRLPVYYCPDCQARGGSETAGVIVALEKPEVCPECGRREPVQD
                     PDVLDTWFSSWLWPFSVLGWPEETDDLKAFYPTQLLVTAPEIIFFWVARMAMAGLEFQ
                     GRLPFGEVYIHGTVRDETGRKMSKSYGNVIDPLEVINEVGADALRFSLISFASQGQDL
                     FIGRKSFALGRNFANKIWNASRLVIARAEAAGGVPEPDPARFSLSQRWILSELEATRR
                     VVERKFDSLRLNEVATALYQFFWHSYCDWYLEMLKVDLDENDDPQSLAVAVLVLDRFL
                     RLLHPLMPFLTEEIWRRLKEADLVRDPAPLLARAAWPANVRGHYRPALNRQMALLLES
                     VSAVRNLKREFNLAPGQPVRAILVLPRPYHKMIGAAAHYLKRLAGVETLELETAHQRR
                     RLEASGLLSRGEIYLPLAGLFDPEREIVRLNKELAVLERELEKGSGRLSSREFLEKAP
                     AEVIERERARQSQLREKADRIKEHLSFLK"
BASE COUNT          476 a          998 c          958 g          496 t
ORIGIN      
        1 ctcccggccc gtcactcgtt ctcccgtgtc atttgagaaa ggaaagatgc tccttgatcc
       61 ggtcggcctt ctcccggagc tgcgactgcc gggcccgttc gcgttcgatc acctcggccg
      121 gcgccttttc caggaattcc cggctcgaca gtcgtccgga acccttctcc agttcccgtt
      181 ccaggacggc cagttccttg ttcaggcgga cgatctcccg ttccggatcg aagaggccgg
      241 ccagcggcag gtaaatttcg ccgcggctca gcaggccgct ggcctccagc cgccgccgct
      301 ggtgcgcggt ctccaattcc agggtctcca ccccggccag tcgcttcaga tagtgggcgg
      361 cggcgccgat catcttatgg tatggcctgg gcagcaccag gatcgcccgg accggttggc
      421 cgggcgcgag gttgaactcc cgtttcaggt tgcgcaccgc gctgaccgac tccagcagaa
      481 gcgccatctg ccggttcagg gccggccggt aatggccccg gacattcgcc ggccaggccg
      541 cccgggccag gagcggcgcc gggtccctga ccaggtccgc ctccttcagc cgccgccaga
      601 tctcttcggt caggaagggc atcagcgggt gcagcaggcg caggaaacgg tccaggacca
      661 gcacggccac cgccagcgac tgcggatcgt cgttctcgtc gaggtccacc ttgagcatct
      721 ccaggtacca gtcgcagtag ctgtgccaga agaactggta gagggcggtc gccacctcgt
      781 tcagccgcag ggaatcaaac ttgcgctcca cgacccggcg ggtcgcctcc agctccgaaa
      841 gaatccagcg ctggctcagg gagaagcggg ccggatccgg ttccggcacg cccccggccg
      901 cctcggcgcg cgcgatgacc agccggctgg cattccagat cttgttggcg aagttgcgcc
      961 ccagggcgaa gctcttgcgg ccgatgaaaa ggtcctgccc ctggctggca aaggagatca
     1021 ggctgaagcg gagggcgtcg gcgccgactt cgttgatgac ctcgagcggg tcgatgacgt
     1081 ttccgtagga cttgctcatc ttgcgcccgg tctcgtcgcg gaccgtaccg tgaatataaa
     1141 cctcgccgaa gggaagccgc ccctggaatt ccaagccggc catcgccatc cgggccaccc
     1201 agaagaagat gatctccggg gcggttacca gcagctgggt cgggtagaag gccttcaggt
     1261 cgtccgtttc ctcgggccag cccaggaccg aaaagggcca gagccaggag gagaaccagg
     1321 tgtccaggac atccgggtcc tggaccggtt cccggcggcc gcattccggg cagacctccg
     1381 gcttctccag ggccacgatg acgccggccg tttcggaacc gccgcgggcc tggcaatccg
     1441 ggcagtaata gaccggcagc cggtggcccc accataactg gcggctgatg caccagtcct
     1501 cgatgttttc aagccagcgc tggtagacct tctgccagcg gcgggggtga aagcggggct
     1561 gccctttccg ggccagttcc agggccggcg cggccaggct cttcatccgc acgaaccact
     1621 ggcgcgaaag gtaaggttcc accaccgttt cgcagcggta acagcggccg accgagtggt
     1681 ggtaatcctc caccttctcg agcaggccgt cctccttcag acaggccacc acgccctccc
     1741 gggccgcgaa tcgttcctgg ccggcgaagg gaccggcggc cgcgttcagg aaaccgcgtt
     1801 cgtcgatcac ggtcaggccg ggcagtccca ggtcccggcc gatgcggaaa tcgttggggt
     1861 catggtgggg ggtcacctta accgcaccgg tgccgaaacc cttctcgacc agcgggtggg
     1921 caaccaccgg tatcggccgg cccaggatgg gcagccggac ctccttgccg accagggcgc
     1981 ggtaacgcgg atcgcgcggg ttgaccgcca cggccgtgtc gccgaagagc gtctccggcc
     2041 gggtggtggc caccaccacc ccgggaccgc cgtcgaggcc cgggtagcgg acatggtaaa
     2101 gatggctctc ctcgggggag tgttcgacct cctcgttggc cagcgcggtg ccgcagcgcg
     2161 ggcaccagtt gactatgtag tcgccccggt agatcagccc ccgctggtaa aggcgccgga
     2221 agcaggtccg gaccgcccgg gagagacccg ggtccatcgt gaagcgctcc cgggaccagt
     2281 cgcaggagca gccgagccgc ttcagctgct cgatgatggt gccgccccgg ctctccttcc
     2341 acttccagac ctcttccagg aatttctccc ggcccaggtc gtcgcggcgc tgccccttct
     2401 ccagcaggtt ccgctccacc acgttctggg tggcgatgcc ggcgtggtcg gtccccggaa
     2461 tccagagggc cttccggccg gccatccgcc gacgccggat gaccaagtcc tggatggtat
     2521 tgttaagggc gtggccgatg tgcaggacgc cggtcacgtt gggcggcgga atgacgatgg
     2581 tgaagggttc gcccgggtcg gccgcttcgg cccggaaggt cggccggccg gcgtattcct
     2641 ggagccagcg cgcctccacc tcccgggcgt tatactgttt ttccataggt tcagatcccg
     2701 cccgcgccag agcggcctcc tttgataatt ttaattattt tgattattaa gataatggcg
     2761 gtcggtccgg gctcaggcct gaaccggttc ggccggaacg gcctccggcg cggccggctc
     2821 atccgcctcc agcaggttcc ggtagacccg ggccatggcc agcatggtgg tcggcaccgt
     2881 gacaaaaagg cccaccacca gggccagcag ccccagaatg ttgacgcc
//
LOCUS       DAOQYN010000219         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_36627_length_2912_cov_4.118656, whole genome shotgun sequence.
ACCESSION   DAOQYN010000219 DAOQYN010000000
VERSION     DAOQYN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36627_length_2912_cov_4.118656"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PKN80_07930"
     CDS             complement(<1..1297)
                     /locus_tag="PKN80_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNQ35975.1"
                     /translation="MAKKLLGQVLRENNLVTEEQLRAALEEQQRTGKFLGRTLVDLHF
                     IDEKALRRVLSIQSGTEFIDLNETQVMPEVIAKIPAAVAKTYQILPIRFEEDTIVVAV
                     SDVLNEFVQENLRFIVGQKVRTVLADPKDLDTALEKYYGKEAMSIMDIIDFFSEQQSG
                     EEGGTSGAPGTAAAAGEVSDVTTMEELANQPPVTNLFNLILLQVAKDNASDLHLEPFE
                     NDFRVRYRVDGKLYDLVHPPRDLAFGLFCRFKIMSGMDIAERRVPQDGRLEFRVGGKD
                     IDLRVSTVPTVFGECLAVRVLDRSRMDFRIDNIGLSFEEQEKLSQLIRTPQGIILATG
                     PTGSGKTTTLYASLKEINSPDIKLITTEDPVEYLLPGAVQVAIREKIGLTFASCLRSI
                     LRQDPDVIFVGEIRDLDTAQISIQASLTGHLVVSTLHTND"
     gene            complement(1314..2405)
                     /locus_tag="PKN80_07935"
     CDS             complement(1314..2405)
                     /locus_tag="PKN80_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNQ35976.1"
                     /translation="MAVPLDELLEKIVKEDASDLHLHAHRPPTMRLNGALLDLNKESL
                     LPEDTVGYMKAITSHEHQEELAKIGSTDFGFAFKDLARFRVAVYRQKGEIALTLRLIP
                     YKFLTFQQLGLGQLVVDLLDRPRGLVMVTGPTGSGKTTTLASMINYINENHDRHIITI
                     EDPIEYYHQHKKSIISQRELGVDLPDFAEGLRRGLRQDPDVFLVGEMRDPETIRAAIT
                     AAETGHLVFATLHTTGASRTVDRITESFPIEEQEQIRVMLSVSILAIISQQLLPRSDV
                     KGRVAAYEIMVATPAIANLVREKKTYRIISEIQTGMKQGMKTMDQSLLELYGAGKIDR
                     ATALDASFDREQLDKSLKMVDIDRQQAKA"
     gene            complement(2420..>2912)
                     /locus_tag="PKN80_07940"
     CDS             complement(2420..>2912)
                     /locus_tag="PKN80_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNQ35977.1"
                     /translation="DIALRAALTGHLVFSMLHTNDATGSPTRLVDLGAAPFLVASSLQ
                     GVLAQRLVRTLCKHCKIPDQPTDEERILLNIKENEEVTLCRPGGCDQCNNTGFRGRMG
                     IMEIFTMDDTLRQMVLHKETSSDIRRVAREHGMRTMGEDGLLKVKRGLTTYEEVINAT
                     VLD"
BASE COUNT          529 a          791 c          935 g          657 t
ORIGIN      
        1 cgtcgttggt atgcagggtg gagaccacca ggtggccggt caaactggcc tggattgaaa
       61 tctgggcggt gtccaggtcg cggatttctc cgacgaagat gacatccggg tcctggcgca
      121 ggatggaacg cagacaggag gcgaaggtga gtccgatctt ttcccggatg gccacctgga
      181 ccgcgccggg cagcagatac tccaccgggt cctcggtggt gatcagtttg atgtcgggag
      241 aattgatctc cttgagcgac gcgtacaggg tggtggtttt gccggagccg gtcgggccgg
      301 tcgccaggat gatgccctgg ggggtacgaa tcaactggga gagtttttcc tgctcctcga
      361 aggaaagtcc gatgttgtcg atccggaagt ccatgcggga ccggtcgagc acgcgcaccg
      421 ccaggcattc gccgaagacg gtcgggacgg tcgaaacgcg caggtcgata tccttgccgc
      481 cgacccggaa ttccagacgg ccgtcctggg ggacgcgtcg ttcggcgatg tccatgcccg
      541 acatgatttt aaagcggcag aagaggccga aggccaggtc gcgcggcggg tgcaccaggt
      601 cgtagagctt gccgtccacc cggtagcgga cccggaagtc gttttcgaac ggttccaggt
      661 ggaggtcgct ggcgttgtcc ttggccacct ggagcagaat gaggttgaag aggttggtca
      721 ccggaggctg attggccagc tcctccatgg tggtgacgtc gctgacctcg ccggcggccg
      781 ccgccgttcc gggagcgccc gaggtgcctc cctcctcgcc gctctgctgt tcggagaaga
      841 agtcgatgat gtccatgatg gacatcgcct ccttcccgta atacttttcc agggcggtat
      901 ccaggtcctt gggatcggcc agcaccgtcc ggactttctg gccgactatg aaccgcaggt
      961 tctcctggac aaattcgttg agaacatcgc tgacggccac cacgatggtg tcttcctcga
     1021 accggatcgg aagaatctgg taggttttgg cgaccgcggc gggaattttg gcgatcacct
     1081 cgggcatgac ctgggtttcg ttcaggtcga tgaactcggt gccgctctgg atggacagca
     1141 cccgccggag tgccttttcg tcgatgaaat gaaggtcaac cagggtccgg ccgaggaact
     1201 ttccggtacg ctgctgctcc tcaagggcgg cccgcagctg ctcctcggtg accagattgt
     1261 tttccctgag gacctgtccc agcagttttt ttgccatgga aatccgatcc gtttcaggcc
     1321 ttggcctgct gcctgtcgat atccaccatc ttcaggcttt tgtccagctg ttcgcggtcg
     1381 aaggaggcgt ccagagcggt ggcacgatct attttacccg cgccgtacag ttcaagtaaa
     1441 gattggtcca tggtcttcat gccctgtttc attccggttt gaatctccga gatgatccgg
     1501 taggtttttt tctctcggac caggttggcg atggccgggg tggcgaccat gatttcgtag
     1561 gcggccactc gacccttgac gtcggatcgg ggtaacaact gctgggagat gattgccaga
     1621 atcgaaaccg aaagcataac gcggatctgt tcctgttcct cgatcgggaa ggattcggtg
     1681 atccggtcta cggtccggga ggcgccggtg gtgtgcaggg tggcgaagac caggtggccg
     1741 gtttcggctg cggtgattgc cgcccggatg gtttccgggt cgcgcatttc accgaccagg
     1801 aagacgtccg ggtcctggcg caggccgcgg cgcagcccct cggcgaaatc aggcaggtca
     1861 acccccagct cgcgctggct gatgatcgat tttttatgct ggtgatagta ttcaatcggg
     1921 tcttcaatcg tgatgatgtg ccggtcgtgg ttttcgttga tataattaat catgctggcc
     1981 agggtggtgg ttttaccgga tccggtcgga ccggtgacca tgaccaagcc gcgcggccga
     2041 tcaagcaggt cgactaccag ctgacccagg ccgagctgct gaaaggtcag gaatttgtaa
     2101 gggataagcc gtagcgtcag agcgatttcg cccttctggc ggtaaaccgc cacacgaaac
     2161 cgggccagat ctttgaaggc aaagccaaaa tcagtgctgc cgatcttggc taactcctcc
     2221 tggtgttcgt gcgaggtgat ggccttcatg taaccaacgg tatcctcggg gagcagcgat
     2281 tctttgttca ggtccaggag ggctccgttc aagcgcatgg ttggtggccg atgcgcgtgc
     2341 aggtgaaggt cggaagcatc ctccttgacg attttttcca gcagttcatc taggggcaca
     2401 gccattttga attcctttgt taatccagca cggtggcgtt gatgacttct tcataggtgg
     2461 tcaagcctcg ttttactttg agcagcccgt cttcacccat ggtgcgcatt ccgtgttcgc
     2521 gggcgacccg gcggatatca ctgctggttt ccttgtgcag gaccatctgg cgcagggtgt
     2581 catccatggt gaagatctcc atgattccca tccggccacg aaaaccggtg ttgttacact
     2641 ggtcacagcc gcccggtcga cagagggtaa cttcctcatt ctccttgatg ttcagcagga
     2701 tgcgttcttc gtcggtaggc tggtcgggta tcttgcagtg cttgcagagg gttcgcacca
     2761 ggcgttgggc cagcacgccc tgaagggaag aggcgaccaa aaacggagcc gcgcccaggt
     2821 caaccagacg cgtgggcgag ccggtggcat cgttggtgtg gagcattgag aagaccaggt
     2881 ggccggtgag cgcggcccgg agggcgatat ca
//
LOCUS       DAOQYN010000220         2910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_36659_length_2910_cov_4.618214, whole genome shotgun sequence.
ACCESSION   DAOQYN010000220 DAOQYN010000000
VERSION     DAOQYN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36659_length_2910_cov_4.618214"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKN80_07945"
     CDS             <1..330
                     /locus_tag="PKN80_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ35978.1"
                     /translation="PLVCAASLAVFEAIRKDGLLENTRRQGEYLKKKLEKISPLVREV
                     RGRGLMLGMELKKPGAAVAARALELGLLINCTQGNVLRIMPPLTVTRRELDLGLKILK
                     QALEEAA"
     gene            389..1306
                     /gene="argF"
                     /locus_tag="PKN80_07950"
     CDS             389..1306
                     /gene="argF"
                     /locus_tag="PKN80_07950"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNQ35979.1"
                     /translation="MFTIKPKKDFLQVNDLDGPEITGLLDLASELKERRRRGEKVIPV
                     HRDKLLGMIFTKPSTRTRVSFEAAIMELGGRALTLTAEASQIGRGESPEDTGRVLSRY
                     LDAVAIRTFDQSEVERLARSASIPVINALTDQHHPCQALADFLTIREKAGDLGKVKLA
                     YFGDGNNVCHSLLQAAARLGVEMLAVTPEGYEPNPTVLAKLQKPNRVKVTTDPAVDLK
                     GVNFIYTDVWLSMGEEKQTAKKRAFKNYQVNQKLLKRAAPGAMVLHCLPAHRGEEISA
                     EVIDGPQSIVWDQVENRLHFQKALLAALL"
     gene            1335..2336
                     /locus_tag="PKN80_07955"
     CDS             1335..2336
                     /locus_tag="PKN80_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNQ35980.1"
                     /translation="MKRALERHGLAACLLTLPENVGYFTGLFLEGVWVLADRKEIRLF
                     AQPLAFEEALHYQPGKLPLAPTIEMTGRLKRLRKGPVGLESELPLNTYLRLKEKQPDL
                     NLAATSIPMEIRAVKEPEERALMRRAGRITSAVWRRLKKATRPGLSERELVRILQAEL
                     AARDAEAAFPPVAGAGISGLFPHAQPGGRRVGRNDRVVFDFGARWRGYCSDLTRVAFT
                     GKKDKELLRRYRCLEEAKAAAEAAAGPGVRAAEVDRAGRQVLERHGLGKSFIHAIGHG
                     VGLSVHERPVLAPGSKDVLAPGMVITIEPGIYLPGWGGLRLEDTFLVTEAGLENLTG"
     gene            2333..>2910
                     /gene="amrB"
                     /locus_tag="PKN80_07960"
     CDS             2333..>2910
                     /gene="amrB"
                     /locus_tag="PKN80_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNQ35981.1"
                     /translation="MIRYPVVSGQFYPADPFELKRLVQDCLGEPQTRQAAVGMMAPHA
                     GYTYSGPVAGAVFNLVRIPELVIILGPNHTGRGADFALAPPGRWQTPLGEVEVPDLSG
                     RGFPEPGSRLRIDSEAHRYEHSIEVQLPFLQVSAGGPLTIVPISIASLEVEPILKLGA
                     ELAAFIGTSGTPALVVASSDLTHYEPDRLVRE"
BASE COUNT          555 a          962 c          941 g          452 t
ORIGIN      
        1 cccctggtct gcgcggcctc cctggcggtc ttcgaagcca tccggaagga cggcctgctg
       61 gaaaacaccc ggcgccaggg tgaatacctg aaaaagaaac tggagaaaat ctcccccctg
      121 gtccgggaag tgcggggccg gggcctgatg ctgggaatgg aactgaagaa accgggcgca
      181 gcggtggcgg cccgggcgct ggaactggga ctgctgatca actgcaccca gggcaacgtg
      241 ctgcgcatca tgccgccgtt gacggtaacc cgccgggaac tggacctcgg cctgaagatt
      301 ctcaagcagg ccctggaaga ggcggcctga gacccggacg gagttcccga aaaggagggc
      361 cccgctctgc gggccgaagc gtgaaaaaat gttcacaatc aagccgaaaa aggatttcct
      421 gcaggtgaat gacctggacg gtccggagat aaccgggctg ctggatctgg cctcggagct
      481 caaagagcgg cgccggcgcg gtgagaaggt catcccggtc caccgggaca agctgctggg
      541 aatgatcttt accaagccgt ccacccggac ccgggtctcc ttcgaagccg ccatcatgga
      601 actgggcggc cgggccttga ctctgacggc cgaagcgagc cagatcggcc ggggcgaatc
      661 ccccgaagac accggccggg tgctgtcgcg ctacctggac gcggtggcga tccgaacctt
      721 cgaccagtcc gaagtggaac ggctggcccg gagcgcctcc atcccggtca tcaatgccct
      781 taccgaccag caccacccct gccaggcact ggccgacttc ctgaccatcc gcgagaaggc
      841 cggcgacctg ggtaaggtaa aactggccta tttcggcgac ggcaataacg tctgccactc
      901 gctcctgcag gcggcggccc ggctgggcgt ggagatgctg gcggtcacgc ccgaggggta
      961 cgaaccgaac ccgacggtgc tggccaaact tcaaaaacca aaccgggtca aggttaccac
     1021 cgatccggcg gttgacctga agggcgtcaa tttcatctac accgacgtct ggctgagcat
     1081 gggcgaggag aagcagaccg ccaagaagcg ggccttcaag aactaccagg tcaaccagaa
     1141 actgctgaaa cgggccgcgc ccggcgccat ggttctgcac tgccttccgg cccaccgggg
     1201 tgaggaaatt tccgccgagg tcattgatgg cccccaatca atcgtctggg accaggtcga
     1261 aaaccggctc cactttcaaa aagccctcct ggccgccctg ctctgaagag agcctggcca
     1321 gtcggcgggg caaattgaaa cgggccctgg agcggcatgg cctggccgcc tgcctgctta
     1381 ccctccccga aaacgtcggc tacttcaccg gcctcttcct ggagggcgtc tgggtcctgg
     1441 ccgaccgcaa ggaaatccgt cttttcgccc agccgctcgc cttcgaagag gcccttcatt
     1501 accagccggg aaaactcccg ctggcgccaa ccattgagat gaccggccgg ctaaaaaggc
     1561 tccggaaagg tcccgtcggt ctggaatcgg aacttccgct gaacacctac ctgcgcctca
     1621 aggagaaaca gccggatctt aacctggcgg ccacctcgat cccgatggaa atccgcgcgg
     1681 tcaaggaacc ggaggagcgg gccctcatgc gccgggccgg ccgcataacc tcggcggtct
     1741 ggcgccggct gaaaaaagcg acccgcccgg gcctttccga acgggagctg gtccggatcc
     1801 tccaggccga actggcggcc cgggatgcgg aggcggcctt tccgccggtc gccggcgccg
     1861 ggatctccgg gctcttcccg cacgcgcagc cgggcggccg ccgggtgggc cggaacgacc
     1921 gggtggtctt cgatttcggc gcccggtggc gcggctattg ctccgatttg acacgggtgg
     1981 ctttcaccgg taaaaaagat aaggaactgc tccggcgcta ccgttgcctg gaagaggcga
     2041 aggccgccgc cgaagcggcc gccggaccgg gcgtccgggc ggccgaggtc gaccgggccg
     2101 gccgacaggt gctggagaga cacgggctcg ggaagtcctt cattcacgcc atcggccacg
     2161 gggtgggcct ttcggtgcac gagcggcccg tgctggcgcc gggcagcaaa gacgtgctgg
     2221 ccccgggcat ggtcataacg atcgaaccgg gtatctacct gcccggctgg ggcggcttga
     2281 gattggaaga taccttcctg gtgaccgaag caggattgga aaacctgacc ggatgatacg
     2341 atacccggtg gtcagcggac aattttaccc ggccgacccg tttgagctga aacggctggt
     2401 gcaggattgc ctgggtgagc cccagacccg ccaagcggcc gtcggcatga tggcacccca
     2461 cgccggttac acctactccg gcccggtggc cggcgccgtc ttcaacctgg tccggatccc
     2521 ggaactggtc atcatcctgg gccccaacca taccggccgg ggcgccgatt tcgcgctcgc
     2581 cccgcccggc cgatggcaga cgccgctggg tgaagtcgag gtccccgacc tttccggccg
     2641 gggctttccg gaacccggct cccggctccg gatcgattcc gaagcccacc gctacgagca
     2701 ttcgatcgaa gtccagctcc ccttcctgca ggtaagcgcc gggggcccgc tgaccatcgt
     2761 cccgataagc atcgcctcgc tggaggtgga accgatcctc aagctgggtg cggaactagc
     2821 cgctttcatc ggaacctccg gaaccccggc cctggtggtc gccagttcgg acctgaccca
     2881 ttacgaaccg gaccggctgg tccgggaaaa
//
LOCUS       DAOQYN010000221         2892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_37014_length_2892_cov_3.973211, whole genome shotgun sequence.
ACCESSION   DAOQYN010000221 DAOQYN010000000
VERSION     DAOQYN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37014_length_2892_cov_3.973211"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..122)
                     /locus_tag="PKN80_07965"
     tRNA            complement(48..122)
                     /locus_tag="PKN80_07965"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(88..90),aa:Asn,seq:gtt)
     gene            complement(134..631)
                     /locus_tag="PKN80_07970"
     CDS             complement(134..631)
                     /locus_tag="PKN80_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNQ35982.1"
                     /translation="MVLKGRARVVGNDVNTDYIISGRYKFKTLDKKELASHLLEDLDP
                     DFVKKIQPGDFIVAGNNFGCGSSREQAPLAIKYADIGAVLAGDFARIFFRNAINVGLP
                     LVECDTSGIKEGDELEVDFTAGTVKNLTRGTTANFKPLPRIMLNILSEGGLAPYFQKH
                     HGFEL"
     gene            753..1769
                     /locus_tag="PKN80_07975"
     CDS             753..1769
                     /locus_tag="PKN80_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35983.1"
                     /translation="MVEIKRNKRPVLVWMNLAEDFQEDPARVPEVVRRLAGAGVTDLC
                     PFAYPPGGRVVYQRSRLMHRARQVDLLGPLVETAHRLGVRVHYVLLPFTHLGEVSLAG
                     LPDPVLVPYLNDQFEKKEYYCVSDPASLVRCLAVLDEVRRDYGVDGFQLDSIRDRGAV
                     LVCQCPRCWKRVEDFYGGRVAPPEEMAGHKSFRVWYAASRKEYVTALVRAVHETAAGL
                     GAEVNMAARSWSGGGAFAEGQDWPDWVLLGLMEMVAPMTYHPADFETFTVEVERQLRL
                     APAGFQNLWPAIGINPGRGSMTGAMFRRQLDWLVERGVSGLSIFQASGLSDELLAILA
                     DRTG"
     gene            1790..2284
                     /locus_tag="PKN80_07980"
     CDS             1790..2284
                     /locus_tag="PKN80_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ35984.1"
                     /translation="MEALEALKKRRSVRHYQEKPVARDLLEQLVDAGRLAASAINIQP
                     WEFVVVTEPAARKRLADTTDHGKFIAEAGACIVVAAKESKYYLEDGAAATQNILVAAA
                     ALGLGTCWVAGDKKPYGPEILQQLGFPGGYRLVSLIAVGYPAQAPALPSKRPLAEVLH
                     WEKF"
     gene            2301..2374
                     /locus_tag="PKN80_07985"
     tRNA            2301..2374
                     /locus_tag="PKN80_07985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2335..2337,aa:Arg,seq:tct)
BASE COUNT          535 a          826 c          971 g          560 t
ORIGIN      
        1 ggggtcttgc gttaaacaag aaccgctgaa atcagcggat tgaattttgg ctccggcggt
       61 aggattcgaa cctacaacct actggttaac agccagccgc gctaccgttg cgctacgccg
      121 gaattgtact actttaaagc tcgaagccgt gatgcttctg gaaataaggc gccaggccgc
      181 cctcggaaag tatgttgagc atgatccggg gcagcggctt gaagttcgcc gtggtgccgc
      241 gggtcaggtt cttgaccgtg ccggcggtga agtccacttc cagttcgtcg ccttccttga
      301 tgccgctggt gtcgcattcg accaggggca ggccgacgtt gatggcgttg cggaagaaga
      361 tccgggcgaa gtccccggcc agcaccgcgc cgatgtcggc gtacttgatg gccagcggcg
      421 cctgctcgcg gctggagccg cagccgaagt tattgccggc cacgatgaag tcgccgggct
      481 ggatcttctt gacgaagtcc gggtccaggt cctccagcag gtggctggcc agctcctttt
      541 tgtcgagcgt cttgaacttg tagcggccgg aaatgatgta atcggtgttg acgtcgttgc
      601 cgaccacccg cgccctgccc ttcagaacca tagctctttt cctcggattt tcttgtttgg
      661 atcgggcgga aagcccatcg gggcgatatt atactacaac cggccatacc ttgccaaatg
      721 gccgcgccag gggataaaat taaaggctgg acatggttga aataaaaaga aataagaggc
      781 cggtcctggt ctggatgaac ctggccgaag atttccagga ggatccggcc cgggttccgg
      841 aggtcgtgcg gcgcctggcc ggcgccggcg tgaccgatct ctgccccttt gcctatccgc
      901 cgggcggccg ggtggtctat cagcgcagcc ggctgatgca ccgggcgcgc caggtggatc
      961 tgcttggtcc cctggtggag acggcccacc ggctgggagt ccgggtccat tatgtcctgc
     1021 tgccctttac ccacctgggc gaggtttcgc tggcggggct gccggacccg gtcctggttc
     1081 cctacctgaa tgaccagttt gagaagaagg agtattactg cgtctccgac ccggccagcc
     1141 tggtgcgctg cctggcggtg ctcgacgagg tgcgccggga ttacggagtg gacggtttcc
     1201 agctcgattc gatccgcgac cggggcgccg tgctggtctg ccaatgtccc cgctgctgga
     1261 agcgggtcga ggatttttac ggcggccggg tggcgccgcc ggaagagatg gccgggcaca
     1321 aatcattccg ggtctggtac gcggccagcc gcaaggagta tgtgaccgcg ctggtccggg
     1381 ccgtgcacga gacggccgcc ggcctggggg cggaggtgaa catggccgcc cggtcctgga
     1441 gcggcggcgg ggcctttgcc gaggggcagg actggcccga ctgggtgctg ctgggcctca
     1501 tggagatggt ggcgccgatg acttatcatc cggccgattt cgaaaccttc acggtggagg
     1561 tggagcggca gctccggctg gcgccggccg gtttccagaa cctgtggccg gcgatcggga
     1621 tcaacccggg ccggggctcg atgaccgggg cgatgtttcg ccgccagctg gattggctgg
     1681 tggagcgcgg ggtcagcggc ctgtccattt tccaggcctc gggattgagc gacgagctgc
     1741 tggccatcct ggccgatcgg actggttgaa aggaacggag gttgagtcta tggaagccct
     1801 ggaagccttg aagaagcggc gcagcgtgcg ccattaccag gagaagccgg tggcgcgcga
     1861 tttgctggag cagctggttg acgccgggcg gctggcggcc agcgccatca acattcagcc
     1921 ctgggagttc gtggtggtca ccgagccggc agcccggaag cggctggccg ataccaccga
     1981 ccacggcaag tttatcgccg aggccggcgc ctgcatagtg gtggcggcca aggagagcaa
     2041 gtattacctg gaggacggcg cggcggccac ccagaatatc ctggtggcgg cggccgccct
     2101 cggcctgggc acctgctggg tggccgggga caagaaaccc tacgggccgg agatactcca
     2161 gcagctgggc tttcccgggg ggtaccggct ggtgagcctg atcgcggtcg gttacccggc
     2221 ccaggcgccg gcgctgccgt cgaagcggcc tctggccgag gtgctgcact gggaaaagtt
     2281 ctaagcggtt ttactgtcac gcgcctgtag ctcaacggga cagagcacct gccttctaag
     2341 caggtggttg ggcgttcgag tcgccccagg cgcgcttttg tttctggccc caccctgccc
     2401 ggccgatttt cttgcctgtg actataccca taagtagaaa gtcaaaaaaa cgaaaaacat
     2461 ggattccctc ttactacgcg agggaatgac gtacaaaaag gcaacaactg ggtctccgcc
     2521 tacgctgaat agcaagcttc ggcggataaa tccgcctacg ctgaatagca agcttcggcg
     2581 gataaatccg cctgcactca aaatgagttt acccgccgga gctgttttac ggcgcaggag
     2641 ggcttcgccg gcaagtccgc gggaatgacg gacttttttc ttgtcattct catacgcgat
     2701 aaatgggaat ccagggttta aatgcctttg aagtatggca ggaagtatgg atttccgact
     2761 acgcccaaaa ggcttcgtcg gacatgcccg ctaactatcc ccctacgcta aacaacaagc
     2821 ggcggcggac aagtccgcgg gaatgaagga cgaaagagca cgtgcgggaa tgacggacaa
     2881 aaaggcaggc gt
//
LOCUS       DAOQYN010000222         2881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_37230_length_2881_cov_3.469214, whole genome shotgun sequence.
ACCESSION   DAOQYN010000222 DAOQYN010000000
VERSION     DAOQYN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37230_length_2881_cov_3.469214"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKN80_07990"
     CDS             complement(<1..626)
                     /locus_tag="PKN80_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ35985.1"
                     /translation="MKQKKRGLVATIGHRLLAFVDYIGSLTILLARVLWALFSGKLRF
                     TSTLVECEIFGNRSFWTVALISLFMGMVVAMESAVPLKLTGGEFYVATIVSISAVREL
                     GPVLTAFLLCGRVGAAIAAEIGTMKITEQVDALQTLAVDPVEYLVLPKFAAAGVMLPI
                     MTIMSDALMIFGGYLVGTTTMNLSSGLYLYQAFRFVLFRDILIGILKA"
     gene            complement(623..1597)
                     /locus_tag="PKN80_07995"
     CDS             complement(623..1597)
                     /locus_tag="PKN80_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNQ35986.1"
                     /translation="MGIVILRSFFVLFAMISGFFLSPDAGGLWGTLYGLGAGLAVIVI
                     EMALENVPARRILVGMMGLITGLVSARLIFDFIFLIPAAESTKLFVRVLLYYLLAYLG
                     VMIALRYSGKWSTIITGHLSEENKKPPLILDSNVIIDGRLVDLIHTNFLDYRLIVPRF
                     VMRELQTIADASNDLKRQRGRRGIESLNRLKKDALADVQIDDIDFPEIESVDAKLIHL
                     AKQSGARILTNDYNLNKIAEVQNIKVLNLNNLATVLKPRYLQGEHLLVKIVKEGREEN
                     QGVGYLDDGTMVVVEGGRRRIGESVEVVIANTIQTSTGRMLFAEPLEK"
     gene            complement(1660..2703)
                     /locus_tag="PKN80_08000"
     CDS             complement(1660..2703)
                     /locus_tag="PKN80_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNQ35987.1"
                     /translation="MKKITLELRVGLFVIISISLILVYAVIKGEINLRRGGYELTAVF
                     QYVAGVDRGAPVRVSGVRVGEVKKVSIDYEKAPLVLLTLYLDRSVKLGHHSKFLIRSY
                     GLIGEKYLEIMPTPITDTPLIQPKSYIYGEEPMPMDRLLTLGEELGRNVRDLAKSLDR
                     VINDPKFKQDIVNTVDSIKNVAEQTTQTMESINSAALKLEEASAQASELMASTQGLVN
                     QSRPFLVQTLLNFQLASAGLAQSTKEMVSLLEKTQSDESSVGKLISSPELYLKLDNSV
                     QKLNRSLEEFQAAATQFKAASENLDALASDVQSGRGSVGKFLKSDELYNEVTGFVSEI
                     RAKPWRLLRPGSK"
     gene            complement(2700..>2881)
                     /locus_tag="PKN80_08005"
     CDS             complement(2700..>2881)
                     /locus_tag="PKN80_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ35988.1"
                     /translation="QFGTTALIVSHDLLFMQGLAERVGLLDQGRIVGFGPLEELQQSS
                     DPLVSNFFRIKGISP"
BASE COUNT          621 a          784 c          871 g          605 t
ORIGIN      
        1 aaggccttga gaattccgat caggatgtcc cggaagagca caaaccggaa ggcctggtaa
       61 aggtaaagcc cgctgctcag gttcatggtg gtggtgccga ccaggtagcc gccgaagatc
      121 atgagcgcgt cggacatgat ggtcattatg ggcagcatta cgccggccgc cgcgaacttg
      181 ggcaggacca ggtattcgac cgggtcgacc gccagcgtct ggagggcgtc cacctgttcg
      241 gtgatcttca tggtcccgat ttcggcggcg atggccgcgc cgacccgacc gcagagcagg
      301 aaagccgtca gcaccggacc cagttcccga accgccgaaa tggagacgat ggtggccacg
      361 tagaactcgc cgccggtcag ttttaagggc acggccgatt ccatggctac caccatgccc
      421 atgaagagcg aaatcagggc gaccgtccag aaggaacggt tgccgaagat ttcgcattcg
      481 accagggtgg aggtgaaacg gagtttaccg gaaaagaggg cccagaggac ccttgccagc
      541 aggatggtca gcgatccgat gtaatcaacg aaggccagga gacggtggcc gatggttgcc
      601 accagcccgc gttttttttg cttcattttt ccagcggttc ggcaaacagc atgcgaccgg
      661 tgctggtttg aatggtgttg gcgatgacca cctcaaccga ttctccgatg cggcgccgtc
      721 cgccttccac caccaccatg gtcccgtcgt cgagatagcc gactccctgg ttctcctcac
      781 gtccctcctt gacgatcttg accagcaggt gttcaccctg caggtagcgc ggcttgagaa
      841 cggtcgccag gttgttcagg ttaagaacct tgatgttctg gacctcggca atcttgttca
      901 ggttgtaatc gttggtcaga atccgggcgc ccgattgttt ggccagatga atgagcttgg
      961 cgtcaacgct ctcgatctcc gggaaatcga tgtcgtcgat ctgcacgtcg gccagcgcgt
     1021 ccttcttcag ccggttgagg ctttcgatgc cgcggcggcc ccgctggcgc ttcagatcgt
     1081 tggaggcgtc ggcgatggtc tgcagttcac gcatcacgaa gcggggaacg atgagccgat
     1141 agtccaggaa attggtgtgg atgaggtcaa ccaggcgtcc gtcaatgatg acattgctgt
     1201 ccaggataag aggcggcttc ttgttctctt cggaaaggtg gccggtgatg atcgtggacc
     1261 acttgccgct gtatctcagg gcgatcatga cgcccaggta agcgagcagg tagtaaagca
     1321 ggacccggac gaagagcttg gtggattcgg cggccgggat cagaaagatg aaatcgaaga
     1381 tgagacgcgc cgagaccaga ccggtgatca aacccatcat gccgaccagg atacgccggg
     1441 ccgggacgtt ttccagcgcc atttcgatga cgatgaccgc cagaccggcg cccagaccat
     1501 aaagggtgcc ccagagtccg ccggcatcgg gagaaaggaa gaacccggaa atcatggcga
     1561 aaagaacaaa aaaacttctc agtattacga tacccataac attctcctcg atacaacctt
     1621 ttgaacagga tgcctgccga aagacggccg ggaacgtttt tattttgagc cggggcgaag
     1681 cagacgccac ggtttggccc ggatttcgct tacgaaaccg gtgacttcgt tgtaaagctc
     1741 gtcgctttta aggaacttcc cgacgctgcc ccggccggat tgaacgtccg aagccaaggc
     1801 atccaggttc tccgaagccg ccttgaactg ggtggccgcc gcttgaaatt cttcaagtga
     1861 gcggttcagc ttttgaaccg aattatcgag tttcaggtag agttcgggag aagaaatgag
     1921 tttcccgacc gaactttcgt cactctgggt tttttccagc agggagacca tttctttggt
     1981 cgattgggcc aggccggccg aagccagctg gaagttgagc agggtctgga ccaggaaagg
     2041 ccgactctga ttgaccagtc cctgggtgga ggccatgagc tccgaggcct gggcggaggc
     2101 ctcttccagc ttgagggcgg ccgagttaat ggattccatg gtttgagtgg tctgttcggc
     2161 gacattcttg atggaatcga cggtattgac gatatcctgc ttgaatttcg ggtcattgat
     2221 gacccggtca agtgatttgg caaggtcgcg cacatttcgg cccagttctt cgcccagggt
     2281 gagcagccga tccatgggca tcggttcttc accgtaaatg tacgatttcg gttggatcag
     2341 cggcgtatcg gtaattgggg tcggcatgat ttccaggtat ttttcaccga tcaatccata
     2401 gctgcggatg aggaacttcg agtggtgccc aagcttgacg ctccggtcca ggtaaagggt
     2461 caacagaacc aacggcgcct tttcgtaatc gatgctgacc tttttaacct cgccgacccg
     2521 gacgccgctt acccggaccg gggcgccccg gtccaccccg gccacgtatt ggaagacggc
     2581 ggtcaattcg taaccgcccc ggcgcaggtt gatttcgccc ttgatcaccg cgtaaaccag
     2641 gatcagcgag atggagatga tgacgaagag tccgacgcgc aattccaggg taatcttctt
     2701 catgggctga ttcccttgat ccggaaaaaa ttgctgacca gcgggtcgga actctgctgc
     2761 agttcctcca gggggccgaa accgacgatg cggccctggt caagcaggcc gacccgttcg
     2821 gccaatccct gcatgaaaag caggtcatgc gagacgatca gggcggtggt gccaaactgc
     2881 t
//
LOCUS       DAOQYN010000223         2842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_37998_length_2842_cov_3.391460, whole genome shotgun sequence.
ACCESSION   DAOQYN010000223 DAOQYN010000000
VERSION     DAOQYN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37998_length_2842_cov_3.391460"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKN80_08010"
     CDS             complement(<1..235)
                     /locus_tag="PKN80_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ35989.1"
                     /translation="MKIRFLGATRNVTGSRFLLESNGQHILVDCGLYQERDFAERNWN
                     PFPFPPARIDAVILTHAHLDHSGYLPKLVMEGFQ"
     gene            complement(377..1225)
                     /locus_tag="PKN80_08015"
     CDS             complement(377..1225)
                     /locus_tag="PKN80_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ35990.1"
                     /translation="MAGFALLLPALFFRLAGKRPAGLRTAAGFLLLVCLVFGGLAFAR
                     NRVWRTELSLWSDNVARAPNKARPRVFLGLAYKHLNQLDRALEQLELAIKLEPRNYYA
                     YCNRGLVYHQLGRHDLALADLSRALEKNPKDPPTLAWRGMVYLDQGALDSALADLDRA
                     AALAPHYAETYFHRGRVRIRKREFEPAIQDFNRMLAHDPGSAITYNQRAIALAGAGQT
                     GAAIADWQEALRRQPDFGEAGFNLALIYYLNRDYQNAWEAVRYLEKIGFPVPGEFLEA
                     LRKESE"
     gene            complement(1450..2313)
                     /locus_tag="PKN80_08020"
     CDS             complement(1450..2313)
                     /locus_tag="PKN80_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ35991.1"
                     /translation="MPEAKSPGQNWLKNPFLPAAAIILLGLLVYAGTFNAAFHFDDMY
                     HLVENPALQGFRRLPELLRYWPTRALAFFTFALNRQLHGLWLPGYHLVNLLLHLAAAL
                     LVRQLALLALAAPRLAGTPAARHRAAVGLFAGLIFVSHPVQTQAVTYIYQRSTLLAAA
                     LYLLALVLYLRCAWTRPAAAPAPVTWAPWPPGCWPCSARRSPSPCPWPCSWPSSASSR
                     PAGAACPGGGWHPSWPCWWWSRSPSGWAGRPPSRTSTGSPPAPPRWPGAITSTPRPAS
                     CSPTCACWYCR"
     gene            complement(2277..>2842)
                     /locus_tag="PKN80_08025"
     CDS             complement(2277..>2842)
                     /locus_tag="PKN80_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNQ35992.1"
                     /translation="ESILTQRANWKNVQQAVANLRAAGSLRLAAVLEMEPERLAGLVR
                     PSGFYRQKTARLQALAGCLLKECGGLAGAGRLETPELRRRLLALNGVGPETADDILLY
                     AFERPVFVIDEYTRRRVRELGWARKFEYRHLQELFESSLPADYRLYQDFHALIVEDGK
                     ATSVRNPAPGKKGKSTCRKPRVRAKTG"
BASE COUNT          466 a          898 c         1061 g          417 t
ORIGIN      
        1 cctggaatcc ctccatgacc agtttgggca ggtatccgct gtggtcgagg tgggcatggg
       61 tgaggatcac cgcgtctatc cgggcgggtg gaaaggggaa ggggttccag ttccgctccg
      121 cgaaatcccg ttcctggtaa aggccgcaat cgaccagtat atgctggccg ttggactcca
      181 gcaggaacct cgaaccggtg acgtttctgg tcgcgcccag gaacctgatt ttcatggcgg
      241 cttctccgta gtttgtcccg gcgcccggag gcgtgtccat gcttccggcg gtcggcgtgg
      301 atgggcgtcc ggctgaatca attataatga ccgtccgtcc ggccgtcaac cggccgcttc
      361 ccggccggtg ccggtcttac tccgactcct tccgcagcgc ctccaggaac tccccgggca
      421 ccgggaaacc gatcttttcc aggtaccgga ccgcctccca ggcgttctga taatcccggt
      481 taagatagta aatcagggcc aggttgaagc cggcctcgcc gaagtccggc tggcgccgca
      541 gggcctcctg ccagtcggcg atggccgccc cggtctgacc ggcgccggcc agggcgatgg
      601 cccgctggtt ataggtgatg gcgctgcccg gatcgtgggc cagcatccgg ttgaagtcct
      661 ggatggccgg ttcgaactcc cgtttccgga tccggacccg gccccggtgg aagtaggtct
      721 ccgcgtaatg cggggccagc gccgcggccc ggtccaggtc ggccagggcg ctgtcgaggg
      781 ccccctggtc caggtaaacc attccccgcc aggccagggt tggggggtcc ttggggttct
      841 tttccagggc ccggctcagg tcggccaggg ccaggtcgtg acggcccagc tggtgataga
      901 ccaggccccg gttgcagtag gcgtagtagt tccggggttc cagcttgatg gccagttcca
      961 gttgttccag ggcccggtcc agctggttca ggtgcttgta ggccagcccc aggaagaccc
     1021 gggggcgggc cttgttgggt gcccgggcga cattgtcgct ccagagggag agctcggtcc
     1081 gccagacccg gttgcgggcg aaggccaggc cgccaaaaac caggcagacc aggagcagga
     1141 agccggcggc cgtccggagg ccggccggcc gcttcccggc cagccggaag aagagcgctg
     1201 gcaggagcag ggcgaagccg gccatcggca ggtagaggcg gtgctcgaag atgacatcct
     1261 gcagcgggat gaagctggac tccaccgaga gggccagcag gaagaagaga atcgcgaagg
     1321 ccggcagccg ctcgcgccgg aagaggcccc cggcggccag cagcatcagc agcagggccg
     1381 accagccgga cagggccgcc ggcgccaggg cccggcgcac gaccgggtag tcgtagtcga
     1441 gcacctgtct taccggcagt accagcaggc gcaggtaggt gagcaggacg cgggcctggg
     1501 tgtagaggta atggcgccag gccagcgggg tggcgctgga ggtgagccgg tcgatgtcct
     1561 cgacggtggg cggccggccc agccagaggg tgagcgggac caccaccagc agggccagga
     1621 agggtgccag ccgccgccag ggcaggcggc gccagccggc ctcgaagaag cagagctcgg
     1681 ccaggagcag ggccaggggc agggtgaagg cgatctcctt gctgaacatg gccagcagcc
     1741 cggcggccag ggcgcccagg taacaggcgc cggcgcggcg gccggccggg tccaggcgca
     1801 gcgcagatag aggaccaggg ccagcaggta gagggcggcg gccaggaggg tggaccgctg
     1861 gtagatgtag gtgaccgcct gggtctggac cgggtggctg acgaagatga ggccggcgaa
     1921 gaggccgacc gcggcccggt gtcgggccgc cggggtgccg gccagccggg gggcggccag
     1981 cgccagcagg gccagctggc ggaccagcag ggcggcggcc aggtgcagca gcaggttgac
     2041 caggtggtag cccggcagcc agagcccgtg cagctggcgg ttcagggcga aggtgaagaa
     2101 ggccagggcc cgggtgggcc agtaccgcaa aagctccggc agccgccgga agccctgcag
     2161 ggccgggttc tccaccaggt ggtacatgtc gtcgaagtgg aaggcggcgt tgaaggtgcc
     2221 ggcgtagacc agcaggccca gcaggatgat ggccgccgcc ggcaggaagg ggttcttcag
     2281 ccagttttgg cccggactct tggcttccgg catgttgact ttcctttctt gcccggcgcg
     2341 ggattacgga cgctggttgc tttcccgtcc tcgacgatca gggcgtggaa gtcctggtaa
     2401 agccggtaat cggccggcag ggacgactcg aagagctcct ggagatgccg gtactcgaac
     2461 ttccgggccc agcccagttc ccggacccgg cgccgggtat actcgtcgat cacgaagacc
     2521 ggccgttcaa aggcgtagag caggatgtcg tcggccgtct ccggcccgac gccgttcagc
     2581 gccagcaggc gccgccgcag ctccggcgtc tccagccggc cggcgccggc caggccgccg
     2641 cactccttca gcaggcagcc ggccagcgcc tggaggcggg ccgtcttctg ccggtagaag
     2701 ccggaggggc ggacaagtcc ggccagtcgt tccggctcca tttcgagcac ggccgccagc
     2761 ctcaggctgc cggccgcccg gagatttgca accgcctgct ggacattctt ccagttggcc
     2821 cgctgggtca ggatggattc ca
//
LOCUS       DAOQYN010000224         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_38299_length_2828_cov_4.473855, whole genome shotgun sequence.
ACCESSION   DAOQYN010000224 DAOQYN010000000
VERSION     DAOQYN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38299_length_2828_cov_4.473855"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PKN80_08030"
     CDS             complement(<1..368)
                     /locus_tag="PKN80_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ35993.1"
                     /translation="MEKNERYDATNIQVLGGIEAVRKRPAMYIGDTSVAGLHHLVYEV
                     VDNSIDESMAGFCTSIKIVIHADGSASVEDNGRGIPVDIHKTEGRPAVEVVMTVLHAG
                     GKFDNSSYKVSGGLHGVGVSV"
     gene            complement(616..>2828)
                     /locus_tag="PKN80_08035"
     CDS             complement(616..>2828)
                     /locus_tag="PKN80_08035"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNQ35994.1"
                     /translation="LHDKFHNSQTNHLETFILAAQLLGPDFRVRDTQSGTVYSGPDLA
                     QLAVNSPKGVRMFITHAYDRNGIYWEMSASYTGYTFNYFEHILTALTGYSDPPGYRPG
                     PGVRDFYRPLRDFQPGRELPEFWRAVLAQPRLSMNSGDCLPVNDARANPTSSQHLELW
                     AKLLGSERLARAAAGVRQFEGKPARGPADPWPPVGSTLMPAAGQATLRGPENRVNLHC
                     DWHPMSDYHSHHDPLNLILSAEKHLLLFDLGYHLGHPLRSLVTDRTAAHNTVTVDREN
                     SIHHRKGVLHHYLGAGPVQFLDASVPEAYPQCDRYRRALVLVGDHYLVDIFRVRGGTR
                     HDYTLLSYAAESRCSLPLKDYPGSLADLRKRYRGYQDLEDDFPNVAGPYQLIHRPRIG
                     KGREGFTVDWPHKGRPDLSLRVHHLNGRGAEVMLARVPFRLRYQGRVETTSEMLIVSR
                     TGRPGLKSTFISVLEPRSRASKPLAGTRRLPLESPDPDAVAVRVDTADGYDIIINGRT
                     DGPHRLAEAGLTLHGKLAVLRRRAGRPGVEVTLYGSRLEGPGLRLAAPPVRSGRVAGI
                     DLARGELTLDEPLPGASQLAGRFILVRGRTGETDRWLVRSAEGRTIRLDLDHSRLLVM
                     EGRVDRVIDRMAFLTGIDFPEPPRPGTPVRFGPAGDFSCPRYHLLMTSQSGNPTRYRL
                     TTCFEIRRYLFGFNREADLRPEFEGRPFWSSAIEIGDDIFTEPYLNREVAGKTP"
BASE COUNT          438 a          872 c         1039 g          479 t
ORIGIN      
        1 accgagacgc ccaccccgtg caggccgccg gagaccttgt agctgctgtt gtcgaacttg
       61 ccgccggcgt ggaggacggt catgaccacc tcgaccgccg gccgcccctc ggtcttgtgg
      121 atgtcgaccg ggatgccgcg cccgttatcc tcgaccgagg cgctgccgtc ggcgtggatt
      181 acgatcttga tgctggtgca gaagccggcc atcgactcgt cgatcgagtt gtcgaccacc
      241 tcgtagacca ggtggtggag gccggcgacc gaggtgtcgc cgatgtacat ggccggccgt
      301 ttgcggacgg cctcgatgcc gcccagcacc tggatgtttg tcgcgtcgta gcgttcgttt
      361 ttttccatat tcaagcgccc tcgggtggtg gaattaaata gaagtctccc caatacattt
      421 aaaataaata aatacaaaaa caggccccaa aacaaccgcg gaaaaagaag ttggcggacc
      481 ggattcgagg tggcgggtat atcttgaggt ccgactggtt taattatact accggttggg
      541 cttgaatgtc aaacgggagc gtcccggggg agacgaccgg ttcctggcca cctcccccag
      601 gggatttcag ccccgctaag gcgtttttcc ggccacttcc cggttcaggt agggttcggt
      661 gaagatatcg tcgccgattt caatcgcgct gctccagaac ggccttcctt caaactcggg
      721 cctcaggtcg gcttcgcggt tgaatccgaa gaggtagcgc cggatctcga agcaggtggt
      781 cagccggtag cgggtcgggt tgccgctctg gctggtcatc agcaggtggt agcgcgggca
      841 gctgaaatcg ccggccgggc cgaaccggac cggggtgccg ggccggggcg gctccgggaa
      901 gtcgatgccg gtcaggaagg ccatccggtc gatcacccgg tccacccgtc cctccatcac
      961 cagcagccgg ctgtggtcga ggtcgagccg gatcgtccgt ccttcggccg accggaccag
     1021 ccagcggtcg gtctcgccgg tccggccccg gaccaggatg aagcggccgg ccagctgcga
     1081 ggcgccgggc agcggctcat caagggtcag ttcacccctg gccaggtcga tcccggcgac
     1141 ccggccgctc cggaccggcg gcgcggccag ccggagaccg ggtccctcga ggcggcttcc
     1201 gtagagggtg acctcgacgc cgggccggcc ggcgcgccgg cgcaggacgg ccagtttccc
     1261 gtgcagggtc aggcccgctt cggcgagccg gtgcgggccg tcggtgcggc cgttgatgat
     1321 gatgtcgtaa ccgtcggcgg tatccacccg gaccgccacc gcgtccgggt cgggcgattc
     1381 caggggcagg cggcgcgtgc cggccagcgg ttttgacgcc cgggagcggg gttccagcac
     1441 gctgatgaag gtcgatttca ggccgggccg gccggtgcgg ctgacgatca gcatttcgga
     1501 ggtggtctcg acgcgcccct ggtagcggag ccggaaggga acccgggcca gcatgacctc
     1561 ggcgccgcgc ccgttcagat gatgcacccg cagggagagg tcgggccgcc ccttgtgcgg
     1621 ccagtcgacg gtgaagcctt cccggccctt cccgatccgg ggccggtgga tgagctggta
     1681 ggggccggcc acgttgggaa aatcatcctc caggtcctga tagccgcggt agcgcttccg
     1741 gaggtcggcc aggctgccgg ggtaatcctt gagcggcagc gagcagcggc tttcggcggc
     1801 gtaggagagc agcgtgtagt cgtggcgcgt gccgcccctg acccggaaga tgtccaccag
     1861 gtaatggtcg ccgaccagca ccagcgcccg gcggtaccgg tcgcactggg ggtaggcctc
     1921 cggcaccgag gcgtcgagga actggaccgg cccggcgccg agataatggt gcagcacccc
     1981 cttgcggtgg tggattgagt tctcccggtc gacggtcacc gtgttgtggg cggcggtccg
     2041 gtcggtgacc aggctgcgca acgggtggcc caggtggtag ccgaggtcga agagcagcag
     2101 gtgcttctcg gccgaaagga tcagattgag cgggtcgtgg tggctgtggt agtcgctcat
     2161 cgggtgccag tcgcaatgca ggttgacccg gttctccggc ccgcgcaggg tggcctgccc
     2221 ggcggccggc atcagggtcg agccgaccgg cggccaggga tcggccggcc cccgggccgg
     2281 cttgccctcg aattgccgca ccccggccgc cgcccgggcg agccgctcgg agccgagcag
     2341 tttcgcccag agttccaggt gctggctgga ggtggggttg gcccgggcgt cgttgaccgg
     2401 caggcagtcg ccgctgttca tggaaaggcg cggctgggcc agcacggcgc gccagaactc
     2461 cggcagctcc cggcccggct ggaagtcgcg caggggacgg tagaaatcgc gcacgcccgg
     2521 gcccggccgg taaccgggcg gatcgctgta gccggtcagg gcggtgagaa tgtgttcgaa
     2581 gtagttgaag gtgtagccgg tgtaggaggc gctcatctcc cagtagatgc cgttgcggtc
     2641 gtaggcgtga gttataaaca tccggacgcc cttggggctg ttgacggcca gctgggcgag
     2701 atcggggccg gagtagaccg tgccggactg ggtgtcgcgg acccggaagt ccgggcccag
     2761 cagctgggcg gccaggatga aggtctccag gtggttggtc tgggagttgt ggaacttgtc
     2821 atgcaggt
//
LOCUS       DAOQYN010000225         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_38369_length_2825_cov_4.312996, whole genome shotgun sequence.
ACCESSION   DAOQYN010000225 DAOQYN010000000
VERSION     DAOQYN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38369_length_2825_cov_4.312996"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..884
                     /locus_tag="PKN80_08040"
     CDS             174..884
                     /locus_tag="PKN80_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ35995.1"
                     /translation="MIEQLRASGGLWISSGDQALILDPGPGSLVRAIESVPRLDPAAL
                     TGVLLSHKHLDHSGDVNVMIEAMTSGGFKKKGALFAPADAFDPDPVILHYLAGFPERV
                     EHLKAGATYQLGDIEFTAVKRLNHPVETYGFNFRFGNETVSLISDTGYFEGIEDGFTG
                     RTLIINVVMIEPRSEIRHLSLPEAEMIIGRIRPERAILTHFGRNILTRGPEQLAREMS
                     DRLKLEVQAAVDGLLLQF"
     gene            955..1587
                     /locus_tag="PKN80_08045"
     CDS             955..1587
                     /locus_tag="PKN80_08045"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNQ35996.1"
                     /translation="MDPEKIDYLENSLSTYLDRLAGNDPVPGGGSAAALSLGLGAALF
                     SMSARFSLGRKKFAGFEAEARQILERSESIRRTASELVEADSRIYLDYRKAAARPRED
                     PQRGPAMARAAGDGNQLLLSMIELAGALLELAEPLARKGNPYLISDVGCGVVLARGAW
                     ESARLNILINLAGQPDLPGGMELRRELETLTGRLDRQAQAILELVREKLE"
     gene            1593..2447
                     /locus_tag="PKN80_08050"
     CDS             1593..2447
                     /locus_tag="PKN80_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNQ35997.1"
                     /translation="MAQLLEGKLIAERLRNEAAAEAAELKKSGVVPKLVAVQAGENPA
                     SAVYLKSQKNQCAKVGLDYELRTCPADIGQAELAAEIERLNQDRSVNGIILQMPLPGQ
                     LDGRKLQAAILPEKDVEGITPANLGRLVLAKPRFVPCTAAGVMELLKSTGDNLYGKEA
                     VVVGHSEIVGKPLALLLLAEFVTVSVCHIATGERGHLADHVGRAEILVVAVGKAGLIK
                     GEWIQKGATVIDVGINRVGDKLVGDVEFEAAAERAARITPVPGGVGPLTVAMLIRNTV
                     LAARAALG"
     gene            2462..>2825
                     /locus_tag="PKN80_08055"
     CDS             2462..>2825
                     /locus_tag="PKN80_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydrofolate reductase"
                     /protein_id="HNQ35998.1"
                     /translation="MVLTVQKSREDILKKLKGYRKVFLSGCQACAATCRTGGEPELKE
                     MSEFLAEHGIETTGYTVPDRGCLLPKIKKQLREKQAELDAAEAILVLCCGLGNQVLTE
                     LTKKWVISGADTLFLGPEW"
BASE COUNT          600 a          882 c          852 g          491 t
ORIGIN      
        1 ggttgtcgga atcatcaaaa accatttttt tattgtagca caagaggcgg cggatggaaa
       61 cgtcccggcg gccttcctgt gttataatta atcaaaacag tcacccaatt ttggcgccag
      121 ctcataaaat ggcctttatt aaattccttg gaaccgccgg ggcccgcttc gttatgatcg
      181 agcaattgag ggcctcgggc ggcctctgga tttccagcgg ggaccaggcc ctcatcctgg
      241 atcccggccc cggctccctg gtccgggcca tcgaaagcgt tccccggctt gatcccgccg
      301 ccctcaccgg cgtgctgctc tcccataaac acctggacca ttccggggat gtcaacgtga
      361 tgatcgaagc catgacttcc ggcggcttca aaaaaaaggg cgccctcttc gccccggccg
      421 acgccttcga ccccgatccg gttattttac attatctggc cggttttccg gaacgggttg
      481 agcatctgaa agccggagcg acctaccagc ttggcgacat cgaattcacc gccgtgaaac
      541 ggctcaatca cccggttgaa acctacggct tcaatttcag gttcggcaat gagaccgttt
      601 ccctgatcag cgacaccggg tacttcgagg ggattgaaga cggcttcacc gggcgcaccc
      661 tgatcatcaa cgtcgtgatg atcgaaccgc ggtccgagat ccgccacctt tcccttccgg
      721 aagcggaaat gatcatcggc cggattcgcc cggaacgggc catcctcacc catttcggtc
      781 gcaacatact gacacgcgga ccggaacaac tggcccggga aatgagcgac cggctgaaac
      841 tggaggtcca ggcggccgtt gatggactgc tcctgcaatt ttaacttgac aatattgctg
      901 tcccggcatt aaaatttcat gagtttgtga ttaaaataac aagagatgac caaaatggac
      961 ccggaaaaga ttgattatct tgaaaattcc ttgagcactt acctcgaccg gctggctggt
     1021 aacgatccgg tcccgggcgg cggcagcgcc gcggcgctct cccttggcct gggggccgcc
     1081 ctcttctcca tgagcgcccg tttcagcctg gggcgcaaga agttcgccgg cttcgaagcc
     1141 gaagcccggc aaatactgga acgttcggaa agcatccgtc ggaccgcctc ggaactggtg
     1201 gaggccgaca gtcggatcta tctcgactac cggaaggcgg ccgcccggcc ccgggaagac
     1261 ccgcagcgcg ggccggccat ggccagggcc gccggcgacg gaaaccagct cctgctctcc
     1321 atgatcgaac tggccggcgc cctgctggaa ttggccgaac ccctggccag gaaaggcaac
     1381 ccttacctga tcagcgacgt gggctgcgga gtggtcctgg cccgcggcgc ctgggagtcg
     1441 gcccgtctga acatcctgat caatctcgcc gggcaaccgg acctgcccgg cggaatggaa
     1501 ctgcgccgcg agctggaaac gcttaccggc cgtcttgacc ggcaggcgca ggccattctc
     1561 gaactggtcc gggaaaaact ggagtagcca aaatggccca actgctggaa ggaaagctga
     1621 tcgccgaacg gttgcgcaac gaggcggccg cggaagccgc cgaattgaaa aagtcgggcg
     1681 tggtcccgaa actggtggcg gtccaggccg gcgaaaaccc ggccagcgcc gtttacctca
     1741 agagccagaa gaaccagtgc gccaaggtcg gcctggatta cgaactgcgg acctgcccgg
     1801 ccgacatcgg ccaggcggaa ctggcggccg aaatcgaacg gctcaaccag gaccgctccg
     1861 taaacggaat catactccag atgccgctgc ccggccagct cgacggccgg aaactccagg
     1921 cggcgattct cccggaaaag gatgtcgagg ggatcacccc ggccaacctg ggccggctgg
     1981 tcttggccaa gccgcgcttc gtgccgtgta cggccgccgg cgtcatggaa ctcctgaagt
     2041 ccaccggtga caatctctat ggcaaggagg cggtcgtggt cggccactcc gaaatcgtcg
     2101 gcaaacccct ggccctgctc ctgctggccg aattcgtcac cgtcagcgtc tgccacatcg
     2161 caaccgggga acgcggccac ctggccgatc acgtcggccg ggccgaaatc ctggtggtgg
     2221 cggtgggcaa ggccggcctg atcaagggag aatggatcca aaagggcgcc accgtgatcg
     2281 atgtcggcat caaccgggtc ggggacaagc tggtcggcga cgtcgagttc gaggcggccg
     2341 cggaacgggc cgcccgcata accccggtgc cgggcggcgt cggtccgctc acggtcgcca
     2401 tgctgatcag aaacacggtc ttggccgccc gggccgccct tggttaaaac aggaggaaaa
     2461 gatggtcctg accgtccaga aatcgcggga agacatattg aaaaaactca aggggtaccg
     2521 aaaggtattc ctttccggct gccaggcttg cgccgccacc tgccgcaccg gcggcgaacc
     2581 ggaattgaag gaaatgtcgg aattcctggc cgaacacggg atagaaacca ccgggtacac
     2641 cgtaccggac cgcggctgcc tgctgcccaa gattaaaaaa cagctccgtg aaaagcaggc
     2701 ggagctggat gccgccgagg cgatcctggt cctttgctgc ggcctcggaa atcaggtact
     2761 gaccgagctc accaagaaat gggtcatctc cggagccgac accctcttcc tggggccgga
     2821 atgga
//
LOCUS       DAOQYN010000226         2817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_38536_length_2817_cov_4.964518, whole genome shotgun sequence.
ACCESSION   DAOQYN010000226 DAOQYN010000000
VERSION     DAOQYN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38536_length_2817_cov_4.964518"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKN80_08060"
     CDS             <1..372
                     /locus_tag="PKN80_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNQ35999.1"
                     /translation="INFTGNAEGHDIFSPDLDVVVTDGFTGNCILKAAEGVAEDFSAF
                     LKEQLESSGLAGEPGIRAVYEKFSRRADFAEHSGGFLMGAAGLCVITHGRSGGEAHRR
                     TILRAREAVKQDLRHRLETAF"
     gene            396..911
                     /gene="def"
                     /locus_tag="PKN80_08065"
     CDS             396..911
                     /gene="def"
                     /locus_tag="PKN80_08065"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNQ36000.1"
                     /translation="MEKLRLRCYPDRVLKQKALEIANIDGSIRSLSQEMLRIMRASRG
                     VGLAANQVGCLRNLVVIDELEGLLEKPLVLINPRIIQAEGEMIGEEGCLSLPGMSATV
                     RRHERICAVFLDLDGQKMEIEARGLMARIIQHETDHLAGVLYPQHLPVVTRRSFLREY
                     REKNQDRSRRL"
     gene            1032..1709
                     /locus_tag="PKN80_08070"
     CDS             1032..1709
                     /locus_tag="PKN80_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNQ36001.1"
                     /translation="MAETLNCYLRKEKGKSAVHKMRRDGQIPAVVYGHKSKNQLLSVP
                     EKEFFKLWQKMAGKQVILDLVIHDSSKESRIQGVLQDYQHELLTNRFLHLDFHKIAAR
                     EKLRLTIPIELDGEAPGVKQGGVVDQVLREVEIEALPKDLPEKIVVDINSMNIGDSIY
                     VSNLQLGEGVRILTHANEPIVSVLAPKKVEEEAPAAPVTEEVQPEVISEEVAEERRKK
                     KEETKES"
     gene            1744..2292
                     /gene="pth"
                     /locus_tag="PKN80_08075"
     CDS             1744..2292
                     /gene="pth"
                     /locus_tag="PKN80_08075"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNQ36002.1"
                     /translation="MRLLIIGLGNPEARYLPTRHNLGFRVLDRLAEKTGAAGFREKEN
                     ALVCRAGLPGTELLLAKPTTYVNNSGTAVLALLGREGLDPGRLLVICDDLSIPFGEFR
                     LRGQGSSGGHNGLQSIIDRIGPDFARLRIGIGPAPEGVDWADFVLSPFSPEEESGLPD
                     IIEEAMARITSFVNQKKEEKDV"
     gene            2285..>2817
                     /locus_tag="PKN80_08080"
     CDS             2285..>2817
                     /locus_tag="PKN80_08080"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNQ36003.1"
                     /translation="MFNMIGWAPLIGTAIIGIFVYSLIRKIQAAEEGSDEMRQVAGAV
                     REGAFAYLRRQYTSVSIYALILFAILTVMELSGLLPVLSAAAFLTGTLCSALAGFIGM
                     NIATQTSHRTAAAARTSLNGALRVSFSAGAVMGLTVVALALLNLGFWWILLKWFYRGW
                     PEAEMVRRIGATLFASG"
BASE COUNT          557 a          914 c          876 g          470 t
ORIGIN      
        1 atcaatttca ccggcaacgc cgaagggcat gacatcttca gcccggacct cgatgtcgtg
       61 gtcaccgacg gcttcaccgg caactgcatt ctcaaggccg ccgaaggagt ggccgaggat
      121 ttcagcgcct ttctgaagga acagttggaa agttccggcc tggccgggga acccggcatc
      181 cgggccgtct atgagaaatt ctcccggcgc gccgatttcg ccgaacacag cggcggattc
      241 ctgatgggcg ccgccggcct ctgcgtaatc acccacggcc gctccggcgg tgaggcgcac
      301 cgacgcacca tcctgcgcgc ccgagaagcg gtcaagcagg acctccggca ccgcctggaa
      361 accgccttct gagaaaaatt caccccggct gcacaatgga aaaactccgc ctgcgctgtt
      421 acccggaccg cgtcctgaag cagaaggccc tcgagatagc gaatatcgac ggctcgatcc
      481 ggtccctcag ccaggagatg ctccggatca tgagggcctc ccggggtgtc ggcctggccg
      541 ccaaccaggt gggctgcctc aggaacctcg tggtgatcga cgagctggag ggactgctgg
      601 agaaacccct ggtcctcatc aatccccgga tcattcaggc cgagggggag atgatcggcg
      661 aggaagggtg cctttcgctg cccggcatgt cggccaccgt gcgccgccac gaaagaatct
      721 gcgccgtctt cctggacctg gacggtcaga agatggaaat cgaggcccgg ggcctgatgg
      781 cccgaatcat tcagcatgag accgatcacc tggcgggcgt tctttacccg cagcacctcc
      841 cggtcgtcac ccggcgcagc tttttgcgcg aataccggga gaagaaccag gaccggtccc
      901 gccggctttg accgcaaccg gcttttggtt atataatatc aagataatcc atcgcgctca
      961 tcattcaagc aaatccccct tcgacccggg gcggccgggt ccggggatat ccaggcaaaa
     1021 cgaggcgtaa aatggcggaa acactcaatt gttacctgcg caaggagaag ggcaaaagcg
     1081 ccgtccacaa gatgcgccgt gacggccaga tcccggcggt cgtttacggg cacaagtcca
     1141 agaaccagct cttgagcgtc ccggagaagg agttcttcaa gctctggcag aagatggccg
     1201 gcaagcaggt catcctggac ctggtgatcc acgacagcag caaggagtcc cggatccagg
     1261 gcgttctcca ggactaccag cacgaactgc tcaccaaccg tttcctgcac ctggacttcc
     1321 acaagatcgc cgcccgggaa aagctgcggc tgaccatccc gatcgaactg gacggcgaag
     1381 cgccgggcgt caagcagggc ggcgtggtgg accaggtgct ccgggaagtc gaaatcgagg
     1441 ccctgcccaa ggacctgccc gaaaagatcg tcgtggacat caattccatg aacatcggcg
     1501 attccatcta tgtctccaac ctccagctgg gcgagggagt ccggatcctg acccacgcca
     1561 acgaaccgat cgtctcggtc ctggcgccca agaaggtcga ggaagaggcc ccggcggccc
     1621 cggtgaccga ggaagtccag cccgaggtca tcagcgagga agtcgccgag gaacgccgca
     1681 agaagaagga agaaaccaag gaatcgtgac cgggaccggc cctgacgact gcgagccacg
     1741 gcaatgcgtc tgcttataat cggcctcggc aaccccgagg cccgttacct tccgacccgg
     1801 cacaaccttg gtttccgggt gctggaccgg ctggccgaaa agaccggagc ggccggcttc
     1861 cgggagaagg aaaacgcgct ggtctgccgg gcgggactcc ccgggaccga actcctgctg
     1921 gccaagccga ccacctatgt caacaacagc gggaccgccg tgctggccct gctgggccgg
     1981 gaagggctgg atcccggccg gctcctggtg atctgcgatg acctgagcat ccccttcggc
     2041 gagttccggc tgcgcggcca ggggagttcg ggcggccaca acggcctgca atcgatcatc
     2101 gaccggatcg ggccggattt cgcccggctg cgcatcggga tcgggccggc cccggagggc
     2161 gtcgactggg ccgatttcgt cctgagcccc ttcagcccgg aagaggagtc cggtctcccg
     2221 gacattatcg aagaagccat ggcaagaatc acctcgtttg tcaaccagaa aaaggaggag
     2281 aaggatgttt aatatgatcg gctgggcgcc gctgattgga acggcgatca tcggtatctt
     2341 cgtttattcg ctgatcagga agatccaggc ggccgaagag ggcagcgacg aaatgcgcca
     2401 ggtggccggg gccgtccggg aaggggcctt cgcctatctc cggcgccagt acaccagcgt
     2461 ctccatctac gccctgatcc ttttcgcgat cctgacggtc atggaattgt ccgggctgct
     2521 tccggtcctc tccgcggccg ccttcctgac tgggaccctc tgttcggccc tggccggttt
     2581 catcggcatg aatatcgcca cccagaccag ccaccgcacc gcggcggccg cccgcacctc
     2641 gctcaacggg gcgctccggg tctctttctc cgccggcgcg gtcatgggcc tgacggtggt
     2701 cgccctggcc ctgctcaacc tcggtttctg gtggatcctg ctcaagtggt tctaccgcgg
     2761 ctggccggag gccgagatgg tccgccggat cggcgccacc ctcttcgcca gcggcat
//
LOCUS       DAOQYN010000227         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_38968_length_2798_cov_5.096245, whole genome shotgun sequence.
ACCESSION   DAOQYN010000227 DAOQYN010000000
VERSION     DAOQYN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38968_length_2798_cov_5.096245"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1081)
                     /locus_tag="PKN80_08085"
     CDS             complement(35..1081)
                     /locus_tag="PKN80_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ36004.1"
                     /translation="MEKTRMILAGVGKHGRSWLKVLKEHPAVSLAGVVDVNPANLTAA
                     RAAAGLDESDCFVSLAACLKARPAGVLVNVTPPDQHRAVSLAGLKAGLHILVEKPLAD
                     RLESAAAIVKAARRYDRKLMVAQNYRFTPWARTVQRLTARERCLGAIGSGQVTFQRGP
                     HFGGFREEMDYPLLVDMAIHHFDLMRGFLAEEAVEVYARSFRPGWSWFRHDPAVALVF
                     SLAGGGVVSYFGSWVARGFNTEWNGDWRLEGEKGLLLSCNNRLWLTPAEAPAEVREVP
                     VPGAESGRANSQAEVIDELLAAIREGREPETSGADNLKSLALVFAALESVRRGRPVRV
                     ASLFRKIGFDFEES"
     gene            complement(1103..1735)
                     /locus_tag="PKN80_08090"
     CDS             complement(1103..1735)
                     /locus_tag="PKN80_08090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNQ36005.1"
                     /translation="MFKVSPTRMELLRLKRRLALARRGHRLLKDKEEQLLTNFYRLVE
                     ESRGSREGLENRFRKAAAGYLSVRAGRSEGEMLRFLTPPAYQSRVSLKSSRLLNIEIP
                     NLRAEWTPRPEKFSSRPLDLFDSLGAYRQLAPDILKLAELETTMIRFAVEIERTRRRV
                     NSLEFILIPELERTTRAIELRLEESERENFVRLKHIKRLSESRPAAESPR"
     gene            complement(1752..>2798)
                     /locus_tag="PKN80_08095"
     CDS             complement(1752..>2798)
                     /locus_tag="PKN80_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005890786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNQ36006.1"
                     /translation="TPINPVSRDYPSEFIETGISAIDGLNSLVRGQKLPIFSGSGLPH
                     NRLTAQICRQSRVLGQAEKFGIVFAAMGITFEEADFFIRDFRQTGAIERSVLFINLAD
                     DPAIERIATPRMALTAAEYLAFELGMHILVILTDLTNYAEALREISAARNEVPGRRGY
                     PSYLYTDLASIFERAGRIKERPGSITQMPILSMPEDDKTHPIPDLTGYITEGQIILSR
                     SLHQAGIYPPVDVLPSLSRLRDKGIGPGRTREDHANLASQLFASYARGREARELAVVL
                     GEAALSEIDQAMLRFSDAFEKEFINQGESESRAVLETLDAGWRLLAGLPRSELKRIRT
                     EILDRFLPDPNRNA"
BASE COUNT          480 a          899 c          951 g          468 t
ORIGIN      
        1 attccagccg ttcccgggag acctgttcca tatctcaaga ctcctcgaaa tcaaaaccaa
       61 tcttccggaa aagggaggcg acccggaccg gccgaccccg gcgcaccgat tccagggcgg
      121 cgaagaccag ggccaggctc ttcaggttgt cggccccgga ggtctccggt tcgcgcccct
      181 cgcggatggc cgccagcaat tcgtcaatca cctcggcctg gctgttggcg cggccggatt
      241 cggcgccggg gaccggcacc tcgcgaacct cggcgggcgc ctcggccggg gtcagccaga
      301 gacggttgtt gcaggaaaga agcaggccct tctcaccctc cagccgccaa tcgccgttcc
      361 actcggtatt gaagccccgg gctacccagg aaccgaagta actgaccacg ccgccgccgg
      421 ccagggaaaa gaccagcgcc acggccggat catgccggaa ccaggaccag ccgggccgga
      481 aggaacgggc atagacctcg accgcttcct cggccaggaa accgcgcatc aggtcgaaat
      541 gatggatggc catgtccacc agcaggggat aatccatctc ctcccggaaa ccgccgaaat
      601 ggggaccgcg ctgaaaggtt acctggccgg agccgatggc gcccaggcag cgctcccggg
      661 cggtcagccg ttgaacggtg cgtgcccacg gggtgaaacg gtaattctgg gcgaccatca
      721 gtttccgatc gtagcggcgg gccgccttga ctatggcggc cgccgattcc aggcggtcgg
      781 ccaggggctt ctcgaccagg atgtgcaggc cggccttgag accggccagg gagaccgccc
      841 ggtgctggtc gggcggagtc acgtttacca gaaccccggc cggccgggcc ttcaggcagg
      901 ccgccagcga aacgaagcag tcggattcgt ccaggccggc ggcggcccgg gccgccgtca
      961 ggttggcggg gttgacgtca accaccccgg ccagcgaaac ggccgggtgt tctttcagga
     1021 ccttgagcca ggaccggccg tgcttcccga ccccggccag gatcatccgg gtcttttcca
     1081 tctcagctcc catggcggcg cctcaccggg gactctcggc cgccggccgg ctctcggaga
     1141 ggcgcttgat gtgcttgagc cggacgaaat tctcccgttc cgactcttcc agccgcagtt
     1201 cgatcgcccg ggtcgtccgc tccagttccg gaataagtat gaactccagg ctgttgaccc
     1261 ggcgccgggt ccgttcgatt tcgacggcga aacggatcat ggtcgtctcc agctcggcca
     1321 gtttcaaaat atccggagcc agctgccggt aggcgcccag gctgtcgaaa agatcgagcg
     1381 gccggctgga gaacttttcc ggccggggcg tccactcggc ccgcaggttg ggaatctcga
     1441 tgttgagcag gcgcgacgat ttcaagctga cccgggactg gtaggccggc ggggtcagaa
     1501 aacgcagcat ctccccttcg ctgcggccgg cccgcaccga caggtagccg gcggccgctt
     1561 tccggaagcg gttttccagc ccttcgcgcg aaccgcggct ctcctccacc agccggtaaa
     1621 aattggtcag cagctgctct tccttgtcct tcaggaggcg gtggccgcgc cgggccaggg
     1681 ccaggcggcg cttgagccgc agcaactcca tccgggtcgg gctgacttta aacatgtcgg
     1741 ggcgatccga ttcaggcgtt ccggttggga tccggcagga aacgatccag gatctcggtg
     1801 cggatgcgct tcaactccga acgcggcagg ccggccagca ggcgccagcc ggcgtcgagc
     1861 gtctccagca ccgcccgtga ttcggattcg ccctggttga tgaattcctt ctcaaaggca
     1921 tcgctgaacc tgagcatcgc ctggtcgatc tcggaaaggg ccgcctcgcc caggaccacc
     1981 gccagttcgc gcgcctcgcg gccgcgggcg taagaggcga agagctggct ggccaggttg
     2041 gcgtggtcct cgcgggtccg gcccggcccg atgcccttgt cacgcagccg cgagagcgag
     2101 ggcagcacgt cgaccggcgg gtaaattccg gcctggtgga ggctgcgcga aaggatgatc
     2161 tgcccctcgg tgatgtaccc ggtcaggtcg ggaatcgggt gggtcttgtc gtcctccggc
     2221 attgagagga tcggcatctg ggtgatggaa ccggggcgtt ccttgatccg gccggcccgc
     2281 tcgaaaatgg aggccaggtc ggtgtaaagg tagctgggat aaccgcggcg gccgggcacc
     2341 tcgttgcggg ccgccgaaat ttcgcgcagg gcctcggcgt agttggtgag gtcggtgagg
     2401 atgaccagga tgtgcatgcc cagttcgaag gccaggtact cggccgcggt cagggccatg
     2461 cgcggagtgg cgatgcgttc gatggccggg tcgtcggcca ggttgatgaa aagcacgctg
     2521 cgttcgatgg cgccggtctg gcggaagtcc cggataaaga aatcggcctc ttcaaaggtg
     2581 atgcccatgg cggcgaagac gattccgaat ttttcggcct ggcccagcac ccggctctga
     2641 cggcagatct gggcggtcag ccggttgtgc ggcagaccgc tcccggagaa gatgggcaac
     2701 ttctggccgc gcaccaggct gttgagcccg tcgatggccg aaatgccggt ctcgatgaac
     2761 tccgacgggt aatcgcggct gaccgggttg atcggcgt
//
LOCUS       DAOQYN010000228         2794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_39053_length_2794_cov_4.610807, whole genome shotgun sequence.
ACCESSION   DAOQYN010000228 DAOQYN010000000
VERSION     DAOQYN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39053_length_2794_cov_4.610807"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1288)
                     /gene="prfB"
                     /locus_tag="PKN80_08100"
     CDS             complement(176..1288)
                     /gene="prfB"
                     /locus_tag="PKN80_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156801994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNQ36007.1"
                     /translation="MPIELKILQDKNRDFRERLDQAREMAGLSSKEARRLELEKEMGS
                     DSFWEKSDNRRVIEELKELREELNAFEAAEKQLGDWGVMLELLEAEPDEKLEEEAARQ
                     VAEIEKQLRELHILVTFREPEDRQPAILTFHAGAGGTESCDWVSMLLRMYQRFVASRR
                     WKGEVVDLLPGEEAGVKRATLIVSAPHAYGYLKGEAGVHRLVRISPFDANRRRHTSFA
                     SLAVIPELPPEAEIEVNEKDIEIQTYRAGGAGGQNVNKVETAIRITHLPTGIVVTCQN
                     ERSQHQNREMAMLILKSRLYQLQAEARRKQEDEKRQQAGEIAWGSQIRSYVLQPYQLI
                     KDHRTGYETGNVEAVLDGGLDAFLEAELMYFKTEAR"
     gene            complement(1339..2112)
                     /locus_tag="PKN80_08105"
     CDS             complement(1339..2112)
                     /locus_tag="PKN80_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNQ36008.1"
                     /translation="MGERLERTLEAAELKGRLSLEQLLILGFLAGAYIGFGALLSLVA
                     ASGWHPGFGLERLAMGAAFSLGLILVVGAGAELFTGNCLLVTANCSGRLCGRATLRNW
                     GWVYLANLAGSVFLAAVVYLAGLHLAFEPAGLARKALEIARAKATLPFWTAFWRGVLC
                     NWLVCLAVWMAAGTRDFAGKVLAVFFPVLAFVAMGFEHSVANMFFIPYGLFLSTVPLP
                     GLDLRAFLVGNLLPVTLGNLAGGAFFVGFLYHRAESAGH"
     gene            complement(2124..>2794)
                     /locus_tag="PKN80_08110"
     CDS             complement(2124..>2794)
                     /locus_tag="PKN80_08110"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNQ36009.1"
                     /translation="ISPGRPLPEKLLAEDPETMLAGAENLSKHIENISLFGVPVVVAV
                     NRFPTDFDSEIKALVDFATRAGARAACESQVWGRGGEGGRALAEAVTAAAAEKNDFRF
                     LYPLEASIKEKIETVALKIYGAGRVEYSPLSEEKIRYYSRNGYAGLPICMAKTHLSLS
                     HDPRLKGRPRDFALPIRDIRLAAGAGFLYPLCGEMRTMPGLPSEPAGNRVDLDDEGRV
                     VGLF"
BASE COUNT          475 a          891 c          900 g          528 t
ORIGIN      
        1 ttatcctgac caggccggat tcgaaccggg cccgcgatcc ccaggaattg gccgactggc
       61 ttggaaggcg tttcccgggc tctccggcca ttgtcgccgg gacgccccgg gaagcgctgg
      121 aaacgggctt gggtctgatc gggcgggggg agctgctctg tatctgcggt tctttttacc
      181 tggcctcggt tttgaagtac atcaattccg cctccaggaa ggcgtccaaa ccgccgtcca
      241 gcaccgcttc aacattgccg gtttcgtagc cggtgcggtg gtccttgatt aattggtagg
      301 gctgcagcac gtaggaacga atctggcttc cccaggcaat ctcgccggcc tgctgccgtt
      361 tttcatcctc ctgcttccgg cgcgcctcgg cctgcagctg gtagaggcgg ctcttgagaa
      421 tcagcatggc catttcccgg ttctggtgct gactgcgttc gttctggcag gtgaccacga
      481 tcccggtcgg caggtgggtg atccggatgg ccgtttcgac cttgttaacg ttctggccgc
      541 cggcgccacc ggcccggtag gtctggatct cgatatcctt ttcattaacc tcgatctccg
      601 cctccggggg gagttccggg atgaccgcca gggaggcgaa ggaggtatgg cgccgccggt
      661 tggcgtcaaa aggggagatg cggaccaacc ggtggactcc ggcttcacct ttaaggtagc
      721 cgtaagcatg gggagccgag acgatcaggg tggcccgctt gacgccggcc tcttcccccg
      781 gcagcaggtc gaccacctcc cccttccagc ggcgggaggc cacgaaacgc tggtacatcc
      841 gcagcagcat tgaaacccag tcgcaggatt cggttccgcc ggcgccggcg tgaaaggtga
      901 ggatggccgg ctgccggtct tcgggttccc ggaaggtcac caggatgtgg agttcgcgga
      961 gttgcttttc aatctccgcc acctgtcggg ccgcctcttc ctccagcttt tcgtccggtt
     1021 cggcctcaag cagttccagc atcacgcccc agtcgcccag ctgtttctcg gccgcttcga
     1081 aggcgttcag ttcctcccgc agttccttca attcctcgat cacccggcgg ttgtcggact
     1141 tctcccagaa ggaatccgag cccatttcct tttcaagttc caggcgcctg gcttccttgg
     1201 acgacaaccc ggccatttcc cgggcctgat cgaggcgttc ccggaagtcc cggttcttat
     1261 cctggagaat tttcagctct atcggcatag ttcgcacact ccggcttttt atattgtaca
     1321 acagttcggg cccggccgtt aatggccggc ggattcggcc cggtgataaa ggaaacccac
     1381 gaagaaggcg ccgccggcca ggttgcccag ggtgaccggc agcaggtttc cgaccaggaa
     1441 ggcgcgcagg tcgagcccgg gcagggggac ggttgacagg aataatccgt aggggatgaa
     1501 gaacatgttg gcgacactgt gctcgaaacc catggccacg aaggccagca ccgggaagaa
     1561 gacggccagg actttgccgg cgaagtcccg ggttccggcc gccatccaga cggccaggca
     1621 gaccagccag ttgcacaaga cgccccgcca gaaggcggtc cagaagggta gggtggcctt
     1681 ggcccgggcg atctcgaggg ccttgcgggc cagcccggcc ggttcgaagg ccaggtgaag
     1741 gccggccagg tagacgaccg cggccaggaa gacgctcccg gccaggttgg ccaggtatac
     1801 ccagccccag ttgcgcaggg tagcccggcc gcagagccga ccggagcagt tggccgtgac
     1861 cagcagacaa ttgccggtga agagttcggc gccggccccg accaccagga tgaggccgag
     1921 gctgaaggcg gcccccatcg ccaggcgttc caggccgaag ccggggtgcc agccgctggc
     1981 cgccaccagg gagaggaggg cgccgaatcc tatgtatgcc ccggccagga aaccaaggat
     2041 gagcagctgt tcaagcgaaa gacgcccttt cagctcggcg gcctccagcg ttctttccag
     2101 tcgttcgccc attctccgtc cggttaaaag agaccgacca cccgcccttc gtcgtccagg
     2161 tcaacccggt tgccggccgg ttcgctgggc aggccgggca tggtcctcat ctctccgcag
     2221 agcgggtaga ggaagccggc tccggccgcc agccggatgt cccggatggg aagcgcaaag
     2281 tcccggggcc gccccttcag cctggggtcg tgcgaaaggg aaagatgggt cttggccatg
     2341 cagatcggca ggccggcgta cccgttccgg ctgtaatagc ggatcttctc ctcggagagc
     2401 gggctgtact cgacccgtcc ggccccgtag atcttcaggg ccaccgtttc gatcttctcc
     2461 ttgatggacg cctcgagcgg gtaaaggaaa cgaaaatcgt tcttttcggc ggccgccgcc
     2521 gtgaccgctt cggccagtgc gcggccgcct tcgccgccgc ggccccaaac ctggctttcg
     2581 caggcggccc gggccccggc ccgggtggcg aaatcgacca gggctttgat ttcggagtcg
     2641 aaatcggtcg ggaagcgatt gaccgccacc accaccggaa ccccgaaaag gcttatgttt
     2701 tcaatgtgct tggagaggtt ttcggcgccg gccagcatcg tttccgggtc ttcggccagc
     2761 agtttctcag gcagcggccg gcctgggctt atgc
//
LOCUS       DAOQYN010000229         2781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_39313_length_2781_cov_3.767792, whole genome shotgun sequence.
ACCESSION   DAOQYN010000229 DAOQYN010000000
VERSION     DAOQYN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39313_length_2781_cov_3.767792"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..>2781
                     /gene="rpoB"
                     /locus_tag="PKN80_08115"
     CDS             13..>2781
                     /gene="rpoB"
                     /locus_tag="PKN80_08115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNQ36010.1"
                     /translation="MCANAATARIIPMRGMWLEFRSDGGNVLHAYLGKKRFFASTLFS
                     ALGYNNDKELTKAFFPEGIEKSEGGDLWLATLKKKPLIGQEEAMLEIYAVLRPGYPTS
                     KKKAADFLIQLFFSKESYEFSEAGRSQLNKKLGRNSAEPALEREDVLATVAYLFGLYQ
                     NRGAVDDVDHLGNKRVRMVGELVAEQVEVGLARLSRLIRDRLNLVKAEETTPQELVNS
                     RTFSATIKDFFARSPYSQLLDQTNPLAELTHKRRLTAIGEGVFADRKRIGFEIRDVHY
                     THYGRICPIETPEGGNIGILTSLATHAQVDKLGFLRSPYWVVEKGRVTDRLVYLSADE
                     EDKAYIAQANTLIDEQGCFQEKEVVVRYWDDFIKVPIEKVTHIDISARQVVSVSTSLV
                     PFLEHDDANRALMGSNMQRQALPVLRPQSPLVNTGMESVVVRDSGTVLKSLEDGVVVK
                     VDAEEIVVAKSDARPGELETERVYRLTKFQHLNQDTVFNQRPLVKVGQRVRKDDLLTD
                     GPSVARDGQLALGRNVLVAFMPWRGYNFEDAIILSEKLVREDAFTSIHIKEFELEARE
                     TELGVEEITRDIPNVAEELLGNLDEEGIIRVGAYVKPNDILVGKITPKSETELTPEER
                     LLRVIFGEKAREVDNTSLYAPPGISGIVINVRKFERVTDKDKSGDRANRLQEKIKAVE
                     TRRKELTGRLLHLAVERLAAVLPHNRNLRPSLTALQALKVSDPEKQKLVDQLVGTLQR
                     RVEEIKAECRKEIQHLKQGDDLGVDVVKKIVVQIAIKKPVSVGDKMSGRHGNKGVIAT
                     IVPEADMPFLPDGTPVEVVLNPLGVPSRMNVGQILETHLGWAAKALGYSVTTPIFNST
                     TEAKIEELLLEASQPGDGKVQLYDGMTGDAFRERITCGYMYMMKLFHLADDKIHARSV
                     GSYSLVTQQ"
BASE COUNT          559 a          833 c          885 g          504 t
ORIGIN      
        1 cgagggggat tggtgtgcgc gaatgccgcg acagcccgga tcatcccgat gcgcgggatg
       61 tggctggaat tccggagcga cggcggcaac gttctgcatg cctatctcgg caaaaaacgt
      121 ttctttgcct ccactctttt ttcggccctc ggatacaaca atgataagga actgaccaag
      181 gccttcttcc cggagggcat cgagaagagc gaggggggcg atctctggct ggccaccctg
      241 aagaagaagc ccctgatcgg ccaggaagag gccatgctgg agatttacgc cgttctgcgc
      301 cccggttacc cgaccagcaa gaagaaggcg gccgactttt taatccagct ctttttctcc
      361 aaggaaagtt acgaattttc cgaggcgggc cgcagccagc tgaacaagaa gcttggccgt
      421 aactccgccg aaccggccct ggaacgcgag gacgtgctgg ctacggtggc ctacctgttc
      481 gggctttatc agaaccgggg tgcggtggat gatgttgatc acctgggcaa caaacgggtt
      541 cggatggtgg gcgaactggt tgccgagcag gttgaagtgg gattggcccg cctgtcgcgc
      601 ctcatccggg accggttgaa cctggtcaag gccgaggaga ccacccccca ggaactggtc
      661 aacagccgga ctttttcggc caccatcaag gactttttcg cgcgcagtcc ctattcgcag
      721 ctgctggatc agaccaaccc gctggccgag cttacccaca agcggcgcct gacggccatc
      781 ggcgaagggg tcttcgccga ccgcaagcgg atcggttttg agatccgcga tgttcactac
      841 acccattacg gccgcatctg cccgatcgag acgccggaag gcggcaacat cggcatcctg
      901 acctcgctgg ccacgcacgc ccaggttgac aagctcggtt ttctgcgttc cccctactgg
      961 gtggtcgaga aggggcgggt taccgacagg ctggtttatc tttcggccga cgaggaagac
     1021 aaggcctaca tcgcccaggc caacacgctg atcgacgagc aggggtgttt tcaagagaag
     1081 gaagtggtgg tccgctactg ggatgacttc atcaaggtcc cgatcgagaa ggttacccac
     1141 atagacattt cggcccgcca ggtggtcagc gtttcgacct cgctggtgcc tttcctggag
     1201 catgacgacg ccaaccgggc cctgatgggc tccaacatgc agcggcaggc gctgccggtg
     1261 ctgcgcccgc agtccccgct ggtcaacacc ggcatggaat cggtggtggt gcgcgattcc
     1321 gggacggtcc tcaagtcgct cgaggacggg gtggtggtca aggttgacgc cgaggagatc
     1381 gtggtggcca agtccgacgc ccggcccggc gaactggaga ccgagcgggt ttatcggctg
     1441 accaagtttc agcacctcaa ccaggatacc gttttcaacc agcgtccgct ggtgaaggtg
     1501 ggccagcggg tcaggaagga cgacctgctt accgacggtc cttcggtggc ccgggacggc
     1561 cagttggccc tcggccgcaa cgtgctggtc gctttcatgc cctggcgcgg ctacaacttt
     1621 gaagacgcca tcatcctgag cgagaagctg gtgcgcgaag acgccttcac ctcgattcac
     1681 atcaaggaat tcgagctcga ggcgcgggag accgagctgg gcgtcgagga gatcacccgg
     1741 gatattccga acgtggccga ggaactgctg ggcaatctgg acgaggaggg tatcatccgg
     1801 gtcggcgcct acgtgaagcc caacgatatt ctggtgggca agatcacgcc caagagcgag
     1861 accgagctga cccccgagga acgcctcctg cgggtcatct tcggcgagaa ggcgcgcgag
     1921 gtcgacaaca cctccctgta cgcgccgccg ggcatctccg ggatcgtcat caatgtacgc
     1981 aagtttgagc gggtgaccga caaggacaag tccggagacc gggccaaccg tctccaggag
     2041 aagatcaagg cggtcgaaac ccggcggaag gaattgaccg gccggctgct gcacctggcg
     2101 gttgaacgtc ttgcggctgt cctgccccac aaccggaacc tgcgtccttc gctgaccgct
     2161 cttcaggccc tcaaggtttc cgatcccgag aagcagaagc tggtcgacca gctggtcggc
     2221 accctccagc ggcgggtcga ggagatcaag gccgagtgtc gcaaggagat ccagcacctg
     2281 aagcagggtg acgacctggg cgtcgacgtg gtcaagaaga ttgttgtcca gatcgccatc
     2341 aagaagccgg tttcggtcgg cgacaagatg tcgggacgcc atggcaacaa gggcgttatc
     2401 gccaccatcg tgccggaggc cgatatgccc ttcctgccgg acggcacccc ggttgaggtg
     2461 gtgttgaatc cgctcggggt tccctcccgg atgaatgtcg gccagatcct tgagacccac
     2521 ctgggctggg cggccaaggc gctcggttac tcggtgacca ccccgatctt caacagcacc
     2581 accgaggcga agatcgagga actgctcctg gaagccagtc agccgggcga cggcaaggtt
     2641 cagctttacg acggcatgac cggcgacgct ttccgggaac ggatcacctg cggttacatg
     2701 tacatgatga agcttttcca cctggccgat gacaagattc acgcccgcag cgtcggttcc
     2761 tactcgctgg ttacccagca g
//
LOCUS       DAOQYN010000230         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_39968_length_2751_cov_5.043027, whole genome shotgun sequence.
ACCESSION   DAOQYN010000230 DAOQYN010000000
VERSION     DAOQYN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39968_length_2751_cov_5.043027"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..1265)
                     /locus_tag="PKN80_08120"
     CDS             complement(294..1265)
                     /locus_tag="PKN80_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ36011.1"
                     /translation="MTYQARAVFYRQLGSMLKAGLPLVNALEHLKGSAGPGGRLFEAL
                     YGGVVSGVSLSETAASLASPSFSLFETASLRAGDASGNLPEVLNRLGDYFDFIHRARS
                     RIINGLVYPAFLLHAAIIIPAVPLLFTKGFGAFLAATLPALAILYGLAAAVVFLARKS
                     GPASAFSARFNRVVLAIPVVGSLARKLALLRFLQAFVCLYTAGIEVREAVELAARTMG
                     NAAMEEEMLHSVVLLRNSATISEAFAENPFLPPVLRDMIRVGAVSGKMDETLTSVIGY
                     LQEDVDNTTAKLVSLLPVIVYLLVAVYIGYVIISFYLGYLNQVMTEL"
     gene            complement(1428..>2751)
                     /locus_tag="PKN80_08125"
     CDS             complement(1428..>2751)
                     /locus_tag="PKN80_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNQ36012.1"
                     /translation="VARGWGKCCIVGCGALHIEAGQRRLTVDGRTFKEGDILTLNGTR
                     GYVYEGRLSMINATENPRFIHFMKMANSFRKLGVRTNADTPDDAARAREFGAEGIGLF
                     RTEHMFYGKNSEKPLFLLRKMILSSTVEERRAALADLFVFVKNDIKATLQVMNGLPVT
                     VRLLDPPLHEFVPTREDERRKLAESLAIDAAEFNRRVAALHETNPMMGHRGVRLGVTY
                     PEITEMQVRAILEATAELVKTGKKALPEIMVPVVGLAAELQDQKEIVDRVYAEVCAKY
                     GLKKIAYLYGTMIEIPRAALLADRIAETAEFFSFGTNDLTQMGFGFSRDDIGGFVPAY
                     LDKKILPEDPFQVLDQAGIGQLVEMGVQRGRSVRPHLKVGICGEHGGEPESVKFCHRV
                     GMNYVSCSPYRVPIAILAAAQAAIEDQRRQKKPAAKGKAGSPRKRGRK"
BASE COUNT          504 a          891 c          912 g          444 t
ORIGIN      
        1 cttctccgcc actcccccct tccttcaccc tcccccacac cgcccacccg ccccgcctta
       61 ccagatccag gcaacttccc gcccttcccg atcctggaaa ccggcggccg gctccgggaa
      121 cagccggcaa aacaggcgcc aaagattccc ggagggtata aacccccctc acccggattg
      181 gtttgaggcc ttaaaaacga cggggcggcc gaaatccggg gaaatcgacg gattgaagcc
      241 ggtaaattat caaaaaccgg ccgggagaga gggcggggac gggttgcggg caatcagagt
      301 tcggtcatca cctggttcag gtagcccagg tagaaggaga tgatgacata cccgatgtaa
      361 acggccacca gcaggtagac gatcaccggc aggagggaga ccagcttggc cgtcgtgtta
      421 tcgacatcct cctggagata gccgatgacg ctggtaaggg tctcgtccat cttgccgctc
      481 accgcgccca ccctgatcat gtcgcggagg accgggggca ggaaaggatt ttcggcgaag
      541 gcctcggaaa tggtcgcgga gttgcgcagc agaaccaccg aatggagcat ttcctcctcc
      601 atggccgcgt tgcccatcgt gcgggcggcc agttcgaccg cctcccgcac ctcgatgccg
      661 gcggtgtaaa ggcagacgaa ggcctggaga aaacggagca gggccagttt cctggccagg
      721 gacccgacga ccggaatcgc gaggaccacc cggttgaacc gggccgaaaa ggcggaagcc
      781 ggcccggatt tcctggccag aaagacgacg gccgcggcca ggccgtaaag aatggccagg
      841 gccggcagcg tcgcggccag gaaggcgccg aagcccttcg tgaagaggag cggaacggcg
      901 ggaatgatga tcgccgcgtg cagcagaaag gcgggataaa ccaggccgtt gatgatccgg
      961 gagcgggccc ggtgtatgaa gtcgaaataa tcccccaacc ggttgagcac ctcgggcagg
     1021 tttccggagg cgtcgccggc ccgcaggctc gccgtctcga agaggctgaa gctcggggag
     1081 gcgagcgaag cggccgtctc ggagagggaa actccggaga caactccgcc gtaaagggcc
     1141 tcgaagagac ggccgccggg gcccgccgag cccttgaggt gctcgagggc gttcaccagg
     1201 ggcagccccg ccttcagcat cgagcccagc tgccggtaaa agaccgcccg ggcctggtag
     1261 gtcatgctcc gccgccttgc ttcatcatct tatgatatca caatcccggc cgtcctttcc
     1321 gatccggccg gaagcgaaaa ccggcaaggc cccggcggtg ataaaaaaac cgggcccctc
     1381 cccggaaagg aaggggcccg gatctttccg gttcgccggc gccggactta cttccggccc
     1441 cgcttccgcg gggaaccggc cttgcccttc gcggccggtt tcttctgccg acgctgatcc
     1501 tcgatcgccg cctgggcggc cgccaggatc gcgatcggca cccggtaggg cgagcagctg
     1561 acgtaattca tgcccacccg gtggcagaac ttgaccgact cgggttcgcc gccgtgttcg
     1621 ccgcagatgc cgaccttgag gtggggccgg acggagcggc cgcgctggac gcccatctcg
     1681 accagctggc cgatgccggc ctggtccagg acctggaagg gatcctcggg caggatcttc
     1741 ttgtccaggt aggcgggcac gaagccgccg atgtcgtcgc gggagaagcc gaagcccatc
     1801 tgggtcaggt cgttggtgcc gaatgagaag aactcggccg tctccgcgat ccggtcggcc
     1861 agcagggccg cccgcgggat ctcgatcatc gtcccgtaga ggtaggcgat cttcttcagg
     1921 ccgtacttgg cgcagacctc ggcgtagacc cggtccacga tctccttctg gtcctgcagt
     1981 tcggcggcga ggccgaccac cggaaccatg atctccggca gggccttctt gcccgtcttg
     2041 accagctcgg cggtggcctc caggatggcc cggacctgca tctcggtaat ctccgggtag
     2101 gtaacgccca gccgcacgcc gcggtggccc atcatcggat tggtctcgtg cagggcggca
     2161 acccggcggt tgaactcggc cgcgtcgatg gccaggcttt cggccagttt ccgccgctca
     2221 tcctcccggg tcggaacgaa ctcgtgcagg ggcggatcaa gcaggcggac ggtcaccggc
     2281 aggccgttca tcacttgcag ggtggccttg atgtcgttct tgacgaagac gaagagatcg
     2341 gccagggcgg cccggcgctc ctcgacggtg ctggaaagaa tcatcttgcg cagcaggaag
     2401 agcggttttt cgctgttctt gccgtagaac atgtgctcgg tgcggaagag gccgatgccc
     2461 tcggcgccga attcccgcgc ccgggcggcg tcgtcgggcg tgtcggcgtt ggtccgcacg
     2521 cccagcttcc ggaaggaatt ggccatcttc atgaagtgga tgaagcgcgg gttctcggtg
     2581 gcgttgatca tcgaaagccg cccctcgtag acgtagccgc gggtgccatt cagggtcagg
     2641 atatcccctt ccttgaaggt ccggccgtcc acggtcagcc ggcgctggcc ggcctcgatg
     2701 tggagggcgc cgcagcccac gatgcagcac ttgccccagc cgcgggccac c
//
LOCUS       DAOQYN010000231         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_41393_length_2692_cov_3.166477, whole genome shotgun sequence.
ACCESSION   DAOQYN010000231 DAOQYN010000000
VERSION     DAOQYN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41393_length_2692_cov_3.166477"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKN80_08130"
     CDS             <1..181
                     /locus_tag="PKN80_08130"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNQ36013.1"
                     /translation="VCKVNIDSDGRLVMTALIRKCLTENPKEFDPRKYLGPARDELKK
                     MYIAKCQMLGSAGKA"
     gene            complement(303..974)
                     /locus_tag="PKN80_08135"
     CDS             complement(303..974)
                     /locus_tag="PKN80_08135"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNQ36014.1"
                     /translation="MARINNLLALVCHQRPDRTLSIAGALLPLCSRCTGIYAGFLLSI
                     AWQWFSGGRRRTLLPPRPALEISAALGILGGLEVFLPGWGIMADGNPARLAAGLLTGT
                     ALALFLLPVFNRFFYQTGPRKERPPRPTSYAFPFLGAALLFLLSSWEHPAAYRLLSAT
                     SLLGLLLVYLLINLMLASMLLDRPKTDTAGRRRQVFLRLLAALLFLGLEILFLRFNPL
                     RLSAG"
     gene            complement(1027..>2692)
                     /locus_tag="PKN80_08140"
     CDS             complement(1027..>2692)
                     /locus_tag="PKN80_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36015.1"
                     /translation="AVLQAGLPLTGDRINRGNAMVSSLFGLNITGTMPSDVTQPDLLT
                     ATQAELDYGLFLAALSQLSQQTGRSIAELLAELNADLLDDSTLNATGSQLLNALEAYL
                     GNANNQTGIGSTDQSGLKDPIKYFSENPVLVPATDISDVWKAKALVSEFRETVLTLNN
                     YTGVGAPGILDTPARRLANELNQELVPELTAALDRLAWVAQWAMLLPGPGSYTFTDYP
                     PYTLTINYGDTGAMNFTVSQDSVVLDSGLLTVEGEAAPIPGLSTLPAGGVVEASFQTP
                     NGRLNIDGGYQFTIGLDSSITLTVNGSITAPGLNIDLLAAAGRGVTLYLLPTADQTSV
                     VPTRLIFNGRAETNTTRLDGYLDAILVENTSTDSGETQVLYLPSSFNLNGSFTELNGG
                     RSTGTVFSGTSAGTWSNAATFNTLLPVSDTNYPVFDATFNGQVSADGRPTVTTFLRAR
                     ESAADLVRFDANYRRQNTDGREVFLSGSGTLNYDTRILTGTFTNQDGVEVEIYFDFNQ
                     PLLTGFINAAGGEKMADISLVGLVPTVTYIDGYSEPILPGLGIPIQ"
BASE COUNT          473 a          718 c         1040 g          461 t
ORIGIN      
        1 ggtctgcaag gtgaacatcg actccgacgg ccggctggtg atgacggcgc tgatccgcaa
       61 gtgcctgact gagaacccca aggagttcga cccgcgcaag tacctgggac cggcccggga
      121 tgaattgaaa aagatgtaca tcgccaagtg ccagatgctg ggaagcgccg ggaaggcgta
      181 agcacggcgg cttgacggcg cagagacgcg ccgggtgtga tttttcaagg ggcgggagat
      241 cgattcccgc cccttttttt atggccgggc gggagtgtgg aatgccttgg tgcgaagcgc
      301 agctaaccgg ctgagaggcg cagcgggttg aagcgcagga agagtatctc gaggccgagg
      361 aagagcaggg cggccagcag gcgtagaaag acctggcgcc ggcggccggc ggtatcggtc
      421 ttcggccggt ccaggagcat ggaggccagc atgaggttga tgagcaggta aacaaggagc
      481 aggcccagga ggctggtagc ggacaggagc cggtaggcgg ccgggtgctc ccaggaactg
      541 aggaggaaga ggagggcggc gccgaggaag gggaaggcgt aggaggtcgg ccggggcggc
      601 ctttctttcc gcggcccggt ctggtaaaag aagcggttaa agaccggcag caggaagagg
      661 gccagcgcgg tgccggtcag cagtccggcc gccaggcggg ccgggttgcc gtcggccatt
      721 attccccagc cgggcaggaa tacttccagg ccgcccagga tgccgagcgc ggccgaaatt
      781 tccagggccg gccgtggcgg cagcagggtg cggcggcggc cgccggagaa ccactgccag
      841 gcgatcgaga gaaggaatcc ggcgtagatg ccggtgcagc ggctgcagag ggggagtagg
      901 gctccggcga ttgacagggt gcggtcgggg cgctgatggc agacgagggc gagcagatta
      961 tttatccggg ccatcaagaa tgaagacgcc gggcgaagct tttcgtttcg cccggcgtcc
     1021 gcgtctttac tggatgggga tcccgaggcc gggcaggatc ggctcggagt aaccgtcgat
     1081 ataggtgacg gtcgggacca ggcccaccag ggagatgtcc gccatttttt caccgccggc
     1141 ggcattgatg aagccggtca gcaggggctg gttgaagtcg aagtagattt ccacctcgac
     1201 accgtcctgg ttggtgaagg tgccggtcag gatcctggtg tcataattca gggtgccgct
     1261 gccggagaga aagacctcgc ggccgtctgt attctggcgc cggtagttgg catcgaagcg
     1321 gaccaggtcg gccgcgcttt cccgggcccg caggaaggtg gtgaccgtcg gccggccgtc
     1381 ggcgctcacc tggccgttga aggtggcgtc gaagaccggg tagttggtat cgctgaccgg
     1441 gagcagcgtg ttgaaggtgg cggcattgct ccaggtgccg gccgaggtgc cgctgaagac
     1501 ggtcccggtc gaccggccgc cgttcaactc ggtgaaggag ccgttcaggt tgaaactgct
     1561 gggcaggtag aggacctggg tttcgccgct gtcggttgag gtgttttcga ccaggatcgc
     1621 gtccaggtag ccgtccaggc gggtggtgtt ggtttcggcc cggccgttga agatcagccg
     1681 ggttggaacc accgaggtct ggtcggcggt gggcagcagg tagagagtga cgccgcggcc
     1741 ggcggcggcc agaagatcga tgttaagacc gggcgcggtg atgcttccgt taacggtaag
     1801 cgtgatggag gagtcgaggc cgatcgtgaa ctggtagccg ccgtctatgt tcaggcggcc
     1861 gttcggggtc tggaagctgg cttcgaccac gccgccggcc ggcagggtcg aaagaccggg
     1921 tatgggcgcg gcctcacctt ccacggttag caggccgctg tccagcacca cgctgtcctg
     1981 gctgaccgtg aagttcatcg ccccggtgtc gccgtagttg attgtcaggg tgtagggcgg
     2041 gtagtcggta aaagtatagc tgcccggacc ggggaggagc atggcccact gggccaccca
     2101 ggccagccgg tcgagggcgg ccgtcaattc ggggaccagc tcctggttga gttcattggc
     2161 caggcggcgg gccggcgtgt ccaggatgcc gggcgctccg acaccggtgt aattgttcag
     2221 ggtgagaacg gtttcgcgga attcggagac cagcgccttg gctttccaga cgtcggagat
     2281 gtcggtggcc ggcaccagga ccggattctc gctgaagtac ttgatcgggt ccttcaggcc
     2341 gctctggtcg gtggacccga tcccggtctg gttgttggcg ttgcccaggt aggcctcgag
     2401 ggcgttcagc agctggctgc cggtggcatt gagcgtggaa tcgtccagca ggtcggcatt
     2461 gagctcagcc agcagttccg cgatggaccg gccggtctgc tgggagagct gggagagggc
     2521 ggccaggaaa aggccgtaat cgagctcggc ctgggtggcg gtgagaaggt ccggctgggt
     2581 gacatccgaa ggcatggtac cggtgatgtt caggccgaag aggctgctga ccatggcgtt
     2641 gccccggttg atccggtccc cggtcagggg caggccggcc tgcaggacgg cc
//
LOCUS       DAOQYN010000232         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_42697_length_2635_cov_3.978682, whole genome shotgun sequence.
ACCESSION   DAOQYN010000232 DAOQYN010000000
VERSION     DAOQYN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42697_length_2635_cov_3.978682"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PKN80_08145"
     CDS             <1..519
                     /locus_tag="PKN80_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36016.1"
                     /translation="FPAPAELAAARKELKRLTAIKGEKAAFKDFYAEVKRNEKRAGRP
                     GPYDSKLHHFVLLKNAQAVIARAAARGESSHLPMELHAIRIGNAAFCTNVFELFLDYG
                     LRIKARSRAARTFVVQLCGGMEGYLPTGRAERHGGYGALINNSIVGSKGGDVLVEKTL
                     EQVNALFSAGSK"
     gene            516..2021
                     /locus_tag="PKN80_08150"
     CDS             516..2021
                     /locus_tag="PKN80_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNQ36017.1"
                     /translation="MKIISIDVGTSSVKAAVYDPEGRELAVSIKEYFLERPRPGWVEA
                     DPEVYWEKTRAALAEVLARTGRSEEIGAVGVASQGETLICLDKAGAPARKALVWLDTR
                     AVEEAHLIESQFGAERIYRVTGQHEISPGYTASLILWLRRHEPEVFARTSKFLLVTDY
                     IVYKLTGRYVSHRALYPSTLYCDFQSGAWWPEMLDFLGIKAAVLPELAASGTAAGRVR
                     CPGLEIPAAAVVCPAPIDQVTGALGAGNFQAGVISESTGTVLALCGVTDTPPYDEQRR
                     FCLFPHAIEGRFVAMPWASASGSILAWFRDNFVPGLDYESLTAEAAAVAAGSDGLTVL
                     PHFEGINLPVRIVEARGVFWGVTPSHRRGHFARAIMESVAFLLKDYVSILEQAGIGGR
                     QVISLGGAARSALWSQIKADVLEKPVVTNRVNETVCLGAAMIAAAGAGLYRDCEEACR
                     KMVQPKRTFTPSEAAGTYRSLYGQYTRLNELHRSFYDGLRDAPGAKEPNHG"
     gene            2014..>2635
                     /locus_tag="PKN80_08155"
     CDS             2014..>2635
                     /locus_tag="PKN80_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36018.1"
                     /translation="MGEKITAGFVGFGEINTPRELVERKCGQAADYLASLGLTVKVVA
                     PVADDPDGKQADRAAGELKRETFDLLVVCLAGWIPSWAVIRSIEAFKDKPVLLWGLTG
                     DRDGDRFVTAAAQAGTTAIRRTLQEMGFRFKYVVDRLGQAAPAERIVSFARAARALSR
                     LRGATVGMAGYRDMRLYGTLYDAASLRARLGVEVEHFELLEVARYMD"
BASE COUNT          432 a          838 c          898 g          467 t
ORIGIN      
        1 tttcccgctc cggccgaact ggccgccgcc cggaaggagc tgaagcggct gactgccatc
       61 aagggtgaaa aggcggcctt caaggacttc tacgcggagg tcaaacgcaa tgagaagcgc
      121 gccggccggc cgggccctta tgacagcaaa ctgcaccatt tcgttctcct gaagaacgcc
      181 caggccgtga tcgcgcgggc cgcggcgcgc ggcgaatcct cccatttgcc gatggaactg
      241 cacgcgatcc ggatcgggaa cgcggctttc tgcaccaacg tcttcgaact ctttcttgac
      301 tacggactgc gtatcaaggc ccgcagccgg gccgcccgga ccttcgtcgt tcagctctgc
      361 ggcggtatgg agggctacct gcccaccggc cgggccgaac ggcacggcgg gtacggcgcc
      421 ctgatcaaca acagtatcgt cggctcaaaa ggcggcgacg tcctggtcga aaagaccctg
      481 gagcaggtca acgcgttgtt ttccgccggt tcgaaatgaa gataatttcc attgacgttg
      541 gcacctcctc ggtcaaggcg gcggtctacg accccgaggg gcgcgaactg gcggtttcta
      601 tcaaggaata cttcctggag cggccgcgtc ccggctgggt ggaggccgat ccggaggttt
      661 actgggagaa gacccgggcg gcgctggccg aggttctggc ccggaccggc cggtcagagg
      721 agatcggggc ggttggcgtg gccagccagg gcgagacgct gatctgcctc gacaaggccg
      781 gggcgccggc aaggaaggcc ctggtctggc tggacacgcg ggccgtcgag gaggcccacc
      841 tgatcgagtc ccagttcggc gcggaacgga tttaccgggt gaccggccag cacgagatct
      901 cgcccggcta cacggccagc ctgattttat ggctcaggcg ccacgagccg gaggtcttcg
      961 cccggacctc gaagtttctc ctggtgaccg attacatcgt ttataaactg accggccgtt
     1021 acgtctccca ccgggccctt tatccctcga ccttatattg cgattttcaa agcggcgcct
     1081 ggtggccgga gatgctcgat ttcctgggga tcaaggcggc cgtcctgccc gaactggccg
     1141 cttcgggcac tgcggccgga agggtccgct gcccgggttt ggagattccg gccgcggcgg
     1201 tcgtctgtcc ggccccgatt gaccaggtga ccggcgcgct gggcgccggc aatttccagg
     1261 ccggcgtcat cagcgagtcc accggcacgg tcctggccct ctgcggggtg accgacaccc
     1321 cgccctacga tgaacagcgc cggttttgtc tctttcccca cgcgattgaa ggccgtttcg
     1381 tggccatgcc ctgggcctcc gcctcgggca gcatcctggc ctggttccgg gataacttcg
     1441 tccccggcct ggattatgag tcgctgacgg ccgaggcggc cgcggtggcg gccgggagcg
     1501 atggcctgac ggtactgccg cacttcgaag ggattaacct gcccgttcgg atcgtcgagg
     1561 cgcgcggcgt cttctggggc gtcacgccat cccatcgcag aggccatttt gcccgggcga
     1621 tcatggagtc ggtcgccttc cttctcaagg attacgtttc catcctggag caggccggca
     1681 tcggcggccg ccaggtcatt tccctgggcg gagcggcccg gagtgcgctc tggtcgcaga
     1741 tcaaggccga cgtcctggag aagccggtgg tcaccaaccg ggtgaacgag acggtctgcc
     1801 tcggggcggc catgatcgcg gccgctgggg ccggcctgta tcgggactgt gaagaggcct
     1861 gccgaaaaat ggttcagccg aagcggacct tcacgccttc ggaagcggcc ggaacctacc
     1921 ggagcttata cgggcagtac acaaggttga acgaactcca ccggagtttt tacgatgggc
     1981 ttcgggatgc tcccggggcg aaggagccga atcatgggtg agaaaatcac ggccggtttc
     2041 gtgggcttcg gcgagatcaa cacgccgcgg gaactggttg agagaaaatg tggccaggcg
     2101 gccgattacc tggcctccct gggactgacc gtcaaagtcg tggccccggt cgcggacgat
     2161 ccggacggga agcaggccga ccgggccgcc ggcgaactga agcgggaaac cttcgatctc
     2221 ctggtcgttt gcctggccgg ctggataccc tcctgggccg tgatcaggag catcgaggcc
     2281 ttcaaggata agcccgtgct tctctggggc ctcaccggcg accgggatgg cgaccggttc
     2341 gtcaccgccg ccgcccaggc gggcaccacg gccatcagga ggaccctgca ggagatgggc
     2401 ttccggttca agtacgttgt tgaccggctt ggacaggcgg cgccggccga gcgcatcgtc
     2461 tccttcgccc gggccgcccg ggcgctgtcc cgcctccgcg gagcgacggt cggcatggcc
     2521 gggtaccggg acatgcgcct ctacgggact ctttacgacg cggccagcct gcgggcccgg
     2581 ctgggtgtcg aggtggagca tttcgagctt ctcgaggtcg cccggtacat ggacg
//
LOCUS       DAOQYN010000233         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_43160_length_2616_cov_3.534166, whole genome shotgun sequence.
ACCESSION   DAOQYN010000233 DAOQYN010000000
VERSION     DAOQYN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43160_length_2616_cov_3.534166"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1536)
                     /gene="hydG"
                     /locus_tag="PKN80_08160"
     CDS             complement(43..1536)
                     /gene="hydG"
                     /locus_tag="PKN80_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNQ36019.1"
                     /translation="MEKTSRLKSWVSGRINQAQIDRYLEAGRDFIPDAELESKLAPAP
                     PPSPARVREIIARALAIQTLEPDETADLMRVRDPELWAELEAAAGQVKRKVYDNRIVT
                     FAPLYLGNRCVNNCLYCGFRSGNDAERRQVLSLDEIRGETEVLAGRIGHKRLIVVYGE
                     HPETGVDYMAESIRVIYGVKVPVGRGFGQIRRVNVNAAPMPVEELKVLAEIGVGTYQV
                     FQETYHRPTYGRVHPAGSIKSDYRWRLYSMHRSLEAGIDDVGLGALFGLYDWRFEVLG
                     LLYHARALEKRFGIGPHTISFPRLEPAVNTPFIQETGYRVSDEDFRKAVTVIRLSVPY
                     AGMILTAREPAPLRRALLPLGCTQLDASSRIGIGAYQAGADEQVSERQQFLLGDTRTL
                     DEVVRELALMGHITSFCTAGYRCGRTGQCIMELLRSGQEGRFCKLNAALTFREWLDDF
                     ATPPTRAAGEPILAREIEEIRQRMPAVFPAFMKHYERISRGERDLYF"
     gene            complement(1490..>2616)
                     /gene="hydF"
                     /locus_tag="PKN80_08165"
     CDS             complement(1490..>2616)
                     /gene="hydF"
                     /locus_tag="PKN80_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNQ36020.1"
                     /translation="LDTAGLDNVSVLAEARLRKTRKILDRSDVITLVTEPGAWGDYER
                     QVVREAEARRTPLVVVVNKIDLQAPAADFLELVGGAAGGRLIACSSLDFTNRDRCLNE
                     FKKHLLAVAPDEFLNPPPLLGDLLPPGGLVAMVVPIDLQAPKGRLILPQVQAIRDALD
                     NDAAVMVVKERELAASLKRLAQPPDLVVCDSQVVLKMVADTPPAVKCTTFSILFARYK
                     GELLEAARGAASLERLQPGDRVLIAEACSHHAIEDDIGRVKIPRWIRQYLGGDIKVEV
                     ASGRDYPENLEEYRVVIHCGGCMLTRREMLFRIHQARARGVAVTNYGLTIAFLQGVIE
                     RVLTPFPAALETFRNARRSLSKEKKHGKNKPLEVLGLRAD"
BASE COUNT          417 a          878 c          877 g          444 t
ORIGIN      
        1 ttgaaaattt tttcaccctc cgttttccaa aggcaggcgg acttaaaaat aaagatcccg
       61 ctcaccccgg ctgatccgtt cataatgctt catgaaggcc gggaagaccg ccggcatccg
      121 ctgccggatc tcctcgatct cccgggccag gatcggctcg ccggcggccc gggtcggcgg
      181 ggtggcgaaa tcatccagcc attcccggaa ggtcagcgcc gcgttcagct tgcagaagcg
      241 cccctcctgg ccgctgcgga gcagttccat gatgcactgc ccggtccgcc cgcagcggta
      301 tccggcggtg cagaaagagg tgatgtggcc catcagggcc agctcccgga ccacctcgtc
      361 cagggtgcgg gtatcgccca gcaggaactg ctgccgttcg gagacctgct cgtcggcccc
      421 ggcctggtag gcgccgattc cgatccggga cgaggcgtcc agctgggtgc agccgagcgg
      481 aagcagggcc cggcggagcg gggccggttc ccgggcggtc aggatcatgc ctgcgtaagg
      541 caccgagagg cggatgacgg tcaccgcctt ccggaaatcc tcgtccgaaa cccggtaacc
      601 ggtctcctgg atgaacgggg tgttcacggc cggttccagc cgggggaaag agatggtgtg
      661 gggaccgatc ccgaaccgct tctcgagggc ccgggcatgg taaagcaggc cgagcacctc
      721 gaagcgccag tcgtaaagtc cgaagagggc gcccagtccg acgtcgtcga tgccggcctc
      781 cagggaccgg tgcatcgaat agagacgcca gcggtagtcg gacttgatgc ttccggccgg
      841 gtgcacccgg ccgtaggtcg gccggtgata ggtctcctgg aagacctggt aggttcccac
      901 gccgatttca gccagcacct tcagctcctc gaccggcatc ggcgcggcgt tgacgttgac
      961 ccgccggatc tggccgaaac cgcggccgac cggcaccttg accccgtaga tgacccggat
     1021 gctctcggcc atgtagtcga cgccggtttc cgggtgctcg ccgtaaacga caatcagccg
     1081 cttgtggccg atccggccgg ccagcacctc ggtctcgccg cggatttcat ccagggagag
     1141 tacctggcgc cgctcggcgt cgttcccgct gcggaaaccg cagtagaggc agttgttgac
     1201 gcagcggttg cccaggtaaa gcggggcgaa ggtcacgatc cggttgtcgt aaaccttgcg
     1261 tttcacctgg ccggcggccg cttccagttc cgcccagagc tccgggtccc ggaccctcat
     1321 caggtcggcc gtctcgtccg gttccagggt ctggatggcc agggcgcggg cgattatctc
     1381 ccggacccgg gccggggagg gcggcggcgc cggggccagc ttcgattcca gttcggcgtc
     1441 gggaatgaaa tcccgtccgg cctccaggta acggtcgatc tgggcctggt taatccgccc
     1501 ggagacccag gacttcaagc ggcttgtttt ttccatgttt cttctccttg ctcaagctcc
     1561 gccgggcgtt ccggaatgtt tccagcgccg ccgggaaagg tgtcagcacc cgttcgatca
     1621 ctccctgcag gaaagcgatg gtcagcccgt aattggtcac cgccacgccg cgggcccggg
     1681 cctgatgaat gcggaaaagc atctcgcggc gggtcagcat gcagccgccg cagtggatca
     1741 ccacccggta ctcctccagg ttctccgggt aatcccggcc ggaggcgact tcgaccttga
     1801 tgtccccgcc caggtactgc cggatccagc gcggaatctt gacccggccg atgtcgtcct
     1861 cgatggcgtg gtggctgcag gcctcggcga tcaggacccg gtcgccgggc tggagccgtt
     1921 cgaggctggc cgccccccgg gccgcctcca ggagctcccc cttgtaacgg gcgaagagga
     1981 tggagaaggt ggtgcacttc accgccggcg gcgtgtcggc gaccatcttc aggaccacct
     2041 gtgaatcgca gacgaccagg tccggcggct gcgccagccg cttcaggctc gcggccagct
     2101 cccgttcctt gacgaccatc acggccgcgt cgttgtccag ggcgtcgcgg atggcctgga
     2161 cctggggcag gatcaggcgg cctttgggcg cctgcaggtc gatcggcacc accatggcca
     2221 ccagtccgcc cggcggcagc aggtccccga gcaggggcgg cggattcagg aactcgtccg
     2281 gcgccaccgc cagcaggtgc ttcttgaact cgttcaggca gcggtcgcgg ttggtaaaat
     2341 cgaggctgga acaagcgatc agccggccgc cggcggcgcc gcccaccagt tccaggaaat
     2401 cggccgccgg ggcctggagg tcgatcttgt tgaccaccac caccagcggc gtccggcgcg
     2461 cctcggcctc gcggaccacc tggcgttcgt agtcgcccca ggcgcccggc tcggtgacca
     2521 gggtgatgac atcggaccgg tccaggatct tcctggtctt ccgcagccgg gcctcggcca
     2581 ggaccgagac gttgtccagg ccggcggtgt ccagga
//
LOCUS       DAOQYN010000234         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_43260_length_2612_cov_4.447399, whole genome shotgun sequence.
ACCESSION   DAOQYN010000234 DAOQYN010000000
VERSION     DAOQYN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43260_length_2612_cov_4.447399"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..867)
                     /locus_tag="PKN80_08170"
     CDS             complement(289..867)
                     /locus_tag="PKN80_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36021.1"
                     /translation="MRQRRLYGAVLIVTALLLVATAAVAGPKSTPAQKARHADRNQDG
                     VLTPQEIRRDKAWSRERKSKANTWWERRADTDNDGRVNPAELEAWKKLKKERLDLNND
                     GEIDARERRLSWRNGRSRVNNWLESGYDANGDGWLEPAESRRLMQDRYTLIKSKGKAK
                     VDTGLEAEYDDNKDGIIDAREAVLMKQDLDLK"
     gene            complement(913..1518)
                     /locus_tag="PKN80_08175"
     CDS             complement(913..1518)
                     /locus_tag="PKN80_08175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019216665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ36022.1"
                     /translation="MKNLMSLLAAGLLLGSVAPLASEEKPLPRGLYAIFETSQGRITC
                     LLYEKEAPKTVRNFTGLALGKKEWTDPKTGRKQKKPLYDGTVFHRVIPDFMIQGGDPL
                     GVGIGGPGYQFEDECTPKLGFDRTGRLAMANAGPNTNGSQFFITVAPTPWLNGKHTIF
                     GQVVSGQEVVDQITRVKRDRNDRPLQDVVLKKVIIEDRTKP"
     gene            complement(1604..>2612)
                     /locus_tag="PKN80_08180"
     CDS             complement(1604..>2612)
                     /locus_tag="PKN80_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014649617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ36023.1"
                     /translation="GNIHIASRTLAKCQAIIDTQKKTGRQLTVTFNYRYAPFNSRIKE
                     IIQSGAIGRVLSVHFEWLLNTQHGADYFRRWHRDKRNSGGLMVHKATHHFDLVNWWIA
                     SAPELVFGLGRLAFYGRENAEERGELNFYYRARGSRWAKDDPFALHLEGNERLEGLYL
                     KAEKEDGYLRDQSVFGDGISIEDDMAVLVRYRNRATMTYHLTAYSPWEGYRVMFNGTR
                     GRLEVEIEERPYVSGAKDDFNMPELRGLEPRDEARPAAIVLRPLWGKPLLVNYEKGSG
                     GHGGGDVRLLDDIFIGPKKDPLGHAAGLYDGVMSIMTGVCANHSFNTGRPIRVDELVK
                     I"
BASE COUNT          379 a          830 c          849 g          554 t
ORIGIN      
        1 gttgcagtag ctggccaccc ttatatttta ctacagcggc gggatatata aaaaagcggc
       61 ggttacggct gccttcggtc tgaccttccc cccgaaaacg gtaccgcttg aaagcaggat
      121 tccggctatg ttagaacata gcgaacaaga gggtacagga tgcgtcagca ttttccgcag
      181 ggtggacgca tctttacgtt tggaataagg aaccccggca acttcctttt gctgaggtgt
      241 aacggaccgg tctgttaggg tccgcctgca agaaaaaacc ggtctcggtt acttcaggtc
      301 aaggtcctgc ttcatcagca ccgcttccct ggcgtcgatg atgccgtcct tgttgtcgtc
      361 gtactcggcc tccagcccgg tgtccacctt ggctttgcct ttgctcttga tgagggtgta
      421 ccggtcctgc atgagccggc ggctttcggc cggttcgagc cagccgtcgc cgttggcgtc
      481 gtagccggac tccagccagt tgttgacccg gctgcggccg ttgcgccagg agaggcggcg
      541 ttccctggcg tcgatctcgc cgtcgttgtt gaggtcgagc cgctcctttt tgagcttctt
      601 ccaggcctcc agttcggccg ggtttacccg gccgtcattg tcggtgtcgg cccggcgttc
      661 ccaccaggtg ttcgcctttg atttccgctc ccgactccag gccttatccc ggcggatctc
      721 ctggggggtc aacactccgt cctggttccg gtcggcgtga cgggccttct gagccggggt
      781 cgatttgggt ccggccacgg ccgccgtggc caccagcagc agcgccgtca cgatcaggac
      841 ggccccatac aaccttctct gcctcatgct ccctcccgtc caggaacaag gcccgccggc
      901 ggcgcgccgg cctcagggct tggtgcggtc ttcgatgatc accttcttca gcacgacatc
      961 ctgcaggggc cggtcgtttc ggtcccgttt cacccgggtg atttgatcga ccacctcctg
     1021 cccggagacc acctggccga agatggtgtg cttgccgttc agccagggcg tgggggcgac
     1081 ggtgatgaag aactgcgagc cgttggtgtt gggcccggcg ttggccatgg ccagccggcc
     1141 ggtccggtcg aagcccagtt tcggggtgca ctcgtcctcg aactggtagc cggggccgcc
     1201 gatgcccacc ccgagcgggt cgccgccctg gatcatgaaa tcggggatga cccggtggaa
     1261 gacggtcccg tcgtaaaggg gtttcttctg cttccggccg gttttgggat cggtccattc
     1321 cttcttgccc agcgccagcc cggtaaagtt ccggaccgtc ttgggcgcct ccttttcgta
     1381 aaggaggcag gtgatccggc cctggctggt ctcgaagatg gcgtagaggc ccctgggcag
     1441 gggtttctct tcactcgcca gcggggcgac cgatcccagc agtagtccgg cggccagcag
     1501 gctcatcagg tttttcatct tctctcccgc ccggccgcgg ccgggcttca ggttgtcggg
     1561 acggcccggc cccgcgcagc gcggttcccg gccggacccg gttttagatc tttaccagtt
     1621 cgtccacccg aatcggccgg ccggtgttga aggaatggtt ggcgcagacg ccggtcatga
     1681 tcgacatcac cccgtcgtag agaccggccg cgtggccgag cgggtccttc ttcggaccga
     1741 tgaagatgtc gtccagcagg cggacgtccc cgccgccgtg accgccggat cccttttcat
     1801 agttgaccag cagcggcttt ccccagagcg gacgcaggac gatcgcggcc ggccgcgcct
     1861 cgtcgcgcgg ctccaggccg cgcagttccg gcatgttgaa gtcatccttg gcgccgctga
     1921 catacgggcg ttcctcgatc tcgacctcca gccgcccccg ggtgccgttg aacatgaccc
     1981 ggtacccctc ccagggcgag taggcggtca ggtggtaggt catggtggcc cggttccggt
     2041 agcggaccag taccgccatg tcgtcttcaa tgctgatgcc gtcaccgaag acgctctggt
     2101 cgcgcaggta gccgtcctcc ttctccgcct tgaggtagag gccttcgagg cgttcgttcc
     2161 cctccaggtg cagggcgaag gggtcatcct tggcccaccg gctgccccgg gcccggtagt
     2221 agaagttgag ttcgccgcgc tcctcggcgt tctcccggcc gtagaaggcg agccggccca
     2281 ggccgaagac cagttccggg gccgaggcga tccaccagtt gaccaggtcg aaatggtggg
     2341 tggccttgtg gaccatcagg ccgccggagt tgcgcttgtc ccggtgccag cggcggaagt
     2401 aatcggcgcc gtgctgggtg ttgagcagcc attcgaagtg gaccgagagc acccggccga
     2461 tggccccgga ctggatgatc tccttgatcc ggctgttgaa gggggcgtag cggtagttga
     2521 aggtgacggt gagctgccgg ccggtctttt tctgggtgtc gatgatggcc tggcacttgg
     2581 cgagcgtgcg cgatgcaatg tgtatgttcc cg
//
LOCUS       DAOQYN010000235         2603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_43504_length_2603_cov_4.069466, whole genome shotgun sequence.
ACCESSION   DAOQYN010000235 DAOQYN010000000
VERSION     DAOQYN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43504_length_2603_cov_4.069466"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /locus_tag="PKN80_08185"
     CDS             complement(<1..85)
                     /locus_tag="PKN80_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ36024.1"
                     /translation="MRKLKKAGFTLIELLVVVAIIAILAALL"
     gene            complement(109..807)
                     /locus_tag="PKN80_08190"
     CDS             complement(109..807)
                     /locus_tag="PKN80_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNQ36025.1"
                     /translation="MRKRGFTLIELLVVIAIIAILAAMLLPALSKAQERARQATCTNN
                     LKQIMLAVLMYMEDYNGWLPYHYGDGNGTGSVPGNWMWSLAHPSNAQYIKNLKVFICP
                     SGKGFPKYYANWPQGYQWPGKYTYGIPYDGWAAMQKCTRFEAERKAAGAGVTIDRLPY
                     AADSSRVVDGTWGGWTYYSITGVPSTANSLTTQYYPRAIHSGKVNMAFMDGHVETVSL
                     DDMSYNTSLRTWWK"
     gene            1141..2112
                     /locus_tag="PKN80_08195"
     CDS             1141..2112
                     /locus_tag="PKN80_08195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNQ36026.1"
                     /translation="MEKRKLGRSGIEVSGLGLGCWAIGGETIQKGKPVGWGKVDDQES
                     IRAIHRAIEMGVNFFDTADTYGAGHSEKVLAVALAGRRDRVVIASKFGYAFNEATRDQ
                     FGPHEDITPEYVTASCEASLRRLKTDYLDLYQFHLGRHERMAEIRDTLEGLVKTGKIR
                     WYGWSTDSPESARFFAAGAHCAAIQQNFSVFGGNRETLAICEENDLASIIRGPLAKGL
                     LTGKFTHQTAFPAEDVRYTWNFREDGDAGRKIDLLDQLRPILTGGGRSLTQGALAYLW
                     AISPQTIPIPGFKTVAQVEENCGALAFGPLTRDEAAKIDRLVATLNG"
     gene            2292..>2603
                     /locus_tag="PKN80_08200"
     CDS             2292..>2603
                     /locus_tag="PKN80_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36027.1"
                     /translation="MNELIFAPVPVPGFAYGAWAAVGLGLMLAVCFAVGFLSRKKAAT
                     FEHFLVGHRDIGPVMTGLALGATWLSGWATLGMMGVTYQVGWSGMWFAGMWSLIGVIP
                     CI"
BASE COUNT          517 a          793 c          791 g          502 t
ORIGIN      
        1 gcagcagggc ggccaggatg gcgatgatgg ccaccaccac caggagttca atcagggtaa
       61 agcccgcctt ctttaatttt ctcatgtttc acctcaccgg atgcgatttc atttccacca
      121 ggtccgcaag gaagtgttgt agctcatgtc atccagactg acggtctcca cgtggccgtc
      181 catgaaggcc atgttgactt ttccactgtg aatggcccgc gggtaatact gggtcgtcag
      241 gctgttggcc gtggagggca cacccgtgat cgaatagtaa gtccagccgc cccaggtacc
      301 gtccaccacc cggctggaat cggccgcgta gggcaggcgg tcaatggtga cgcccgcgcc
      361 ggccgccttc cgctccgcct cgaagcgggt gcacttctgc atcgccgccc agccgtcgta
      421 gggaatgccg taggtgtatt tgcccggcca ctggtatcct tgcggccagt tggcgtagta
      481 tttcgggaaa cccttgccgc tcgggcagat gaagaccttc aggttcttga tgtactgggc
      541 gttggaggga tgggccaggc tccacatcca gttgccgggc acgctgccgg tcccgttccc
      601 gtcgccgtaa tggtaaggca gccagccgtt atagtcctcc atgtacatca ggaccgccag
      661 catgatctgc ttcaggttgt tcgtacaggt cgcctggcgc gcccgttcct gggccttcga
      721 gagagccggc agcagcatcg ccgccaggat cgcgatgatg gcaatgacaa ccaggagttc
      781 aatcagcgta aatcctcttt tcctcatttt tcacctcgat gaaatatatg gattgaaaac
      841 caaccgcctc aatcgcctgg cctcaccccc tttctttttt cttaagattt aaaccggcac
      901 aaattccgcc ccggccaacg ataaccggcg ccagacaaaa tttaattacc ggcacccgta
      961 aatcccaccc taaaagtttc ttcaatataa aaatacaata aaaactttcc gttgtcaagc
     1021 ataggggtta actttgaata aaacatataa ttatataatt gcagcttaat ccggcgtccg
     1081 aaaccgcctg tgttaaaata tagcccggag cggttcacgc cacaccaaaa gggggcaggc
     1141 atggagaagc gaaaactggg acggagcggc atcgaggtca gcggcctcgg cctgggctgc
     1201 tgggccatcg gcggggaaac cattcaaaag ggaaagccgg tcggctgggg aaaggtggac
     1261 gatcaggaat cgatccgggc catccaccgg gcgattgaaa tgggcgtcaa cttcttcgac
     1321 accgccgaca cttacggggc cggccacagc gagaaggtgc tggccgtggc gctggccggc
     1381 cggcgggacc gggtggtcat cgcctccaag ttcggctacg ccttcaacga ggcgacccgc
     1441 gaccagttcg gaccccacga agatatcacg ccggagtatg taacggcctc ctgcgaggcc
     1501 agcctgcggc ggctgaagac cgactatctc gacctttacc agtttcacct cggccgccac
     1561 gagcggatgg ccgaaatccg cgataccctg gaaggcctgg taaagaccgg caagatacgc
     1621 tggtacggct ggtcgaccga cagccccgaa tcggcccggt tcttcgccgc cggggcccat
     1681 tgcgcggcca tccagcagaa cttctcggtc ttcggcggca accgggaaac cctggcgatc
     1741 tgcgaggaga acgacctggc cagcatcatc cgggggccgc tggccaaggg gctgctgacc
     1801 ggcaagttca cccaccagac cgccttcccg gccgaggatg tccggtacac ctggaacttc
     1861 agggaagacg gggacgccgg ccggaagatc gacctgctgg atcaactgcg gccgatcctg
     1921 accggcggcg gccggagcct gacccagggc gccctggctt acctgtgggc gatcagtccc
     1981 cagaccatac ccatccccgg cttcaagacc gtcgcccagg tggaggagaa ctgcggcgcg
     2041 ctggccttcg gacccctcac ccgggacgag gccgccaaga ttgaccgcct ggtcgcgacc
     2101 ctgaacggct gaaaagcaag cgccggagtg gacagggtcc gatttgcctg tgatacaata
     2161 ggggcatcgt atccttatcc aaggaaaggg ggacccgatg ggaatgggat ggtttgcctg
     2221 gtggatggta atcatcgcca gcgtcttgct gatcgtggtc gggcttcgtt tcacgctgcg
     2281 ggggtggaaa gatgaatgag ctgatcttcg ccccggttcc ggtacccggg ttcgcttatg
     2341 gcgcctgggc cgccgtcgga ctcggcctga tgctggcggt ctgcttcgcg gtcgggttcc
     2401 tcagccgcaa gaaggccgcc accttcgaac acttcctggt cggccaccgg gacatcggcc
     2461 cggtcatgac cgggctggcg ctgggggcca cctggctgag cggctgggcg accctgggca
     2521 tgatgggggt cacttaccag gtcggctggt ccggcatgtg gttcgccggc atgtggagcc
     2581 tcatcggggt cattccctgt atc
//
LOCUS       DAOQYN010000236         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_44289_length_2574_cov_4.678841, whole genome shotgun sequence.
ACCESSION   DAOQYN010000236 DAOQYN010000000
VERSION     DAOQYN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44289_length_2574_cov_4.678841"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..814)
                     /locus_tag="PKN80_08205"
     CDS             complement(179..814)
                     /locus_tag="PKN80_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36028.1"
                     /translation="MPLPESKTGTGAAGLLLVWTDASVAGLVSRLESFRASLEAGGAA
                     ALSGLVRSVNADYESSAGEGRLCLALAVRSPAELLERTGQALEKLKGGADSFSEAFGL
                     HFQTRPAAGGRVAFLFPGQGSQRPGMLRELVEAFPELRSAFETADRVLAGRLSRPLSD
                     YVFPSPAAGPEEEKRQLEELTATNVAQPALGAAGFGFSRLLESLGVTAGSI"
     gene            1194..1481
                     /gene="groES"
                     /locus_tag="PKN80_08210"
     CDS             1194..1481
                     /gene="groES"
                     /locus_tag="PKN80_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNQ36029.1"
                     /translation="MKLKPLGDRVVVKPLEAAEKTKSGIVLPDTAKEKPQQGKVEAVG
                     KGRQTDDGKVLPMEVKVGNTILYGKYSGTEVTIDDEEYVVLHESDILAIVE"
     gene            1507..>2574
                     /gene="groEL"
                     /locus_tag="PKN80_08215"
     CDS             1507..>2574
                     /gene="groEL"
                     /locus_tag="PKN80_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNQ36030.1"
                     /translation="MAGKQLTFEQEARQLVLKGVQTLRNAVAPTLGPRGRFVILEKKF
                     GSPTVINDGVTIAKEVELSNPFENMGAQLAREIASKTNDVAGDGTTTSVILAHAIVSE
                     GMKYMAAGSNSVQLRHGIEKAVESVVANLKKMSQPIKGKEEIEQVATVAAANDHEIGK
                     IIADAMDKVGKDGVITVEEAKGTETTLKVVEGMQFDRGYLSPYFITDTERMEAVLEDP
                     FILIYDKKISAIKDILPLLEKTAQVGKPLLIVAEEVEGEALATLVVNKIRGTLLTVAV
                     KAPGFGDRRKAMLDDIAVLTGGKAISEDIGLKLENVDLAMLGRAKRVVVDKENTTIVE
                     GAGKASDITGRINQIKKEIEKT"
BASE COUNT          578 a          811 c          801 g          384 t
ORIGIN      
        1 aaccccgccc gctgcccgga gcggacaaac cctatcacgg cttggcctgg cagatccacc
       61 atgcgaagga atccgtcgat gaagcccgca agctgctgcc ggagatggcc gccctgggcg
      121 ccgacgccgt cctcatcagc aacgcaggct accaggagca cgcgggctcc gacacgtttc
      181 agatcgaccc ggccgtcacc cccagcgact ccagcaggcg gctgaacccg aaaccggccg
      241 cgcccaaggc cggctgggca acgttggtgg ccgtcaattc ctccagctgc cgcttctcct
      301 cttcggggcc ggcggccggc gaaggaaaca cgtaatcgct caacgggcgg gacaaacgac
      361 cggccaggac ccgatcggcc gtctcgaagg ccgaccgcag ttccggaaag gcctcgacca
      421 gctcccggag catgccgggc cgctgggagc cctgaccggg aaagaggaag gccacccggc
      481 cgccggccgc cggccgggtc tggaaatgca ggccgaacgc ctcgctgaaa gaatcggccc
      541 cgcccttaag cttttcgagc gcctggccgg tccgttccag gagttccgcc ggggaacgaa
      601 ccgccagcgc caggcataac cggccctcgc ccgcggagga ctcgtagtcg gcgttgacgg
      661 agcggaccag gccggacagg gcggcggcgc cgcccgcctc cagcgaggcc cggaatgatt
      721 ccagccgtga aaccaggccg gccaccgagg cgtccgtcca gaccagcaga agaccggcgg
      781 cgccggtccc ggttttcgat tcaggcagag gcatgccacc ctccttttat cttgaaaaac
      841 gcggaaaagc ccgacagcgg ccggctttcc gctatttcgc gccgggaagc caatacaata
      901 taaacggagg ccgaaccgct tgtcaaattg gcctccggac cctgcctgta acctttcctc
      961 tgcctgatca tcaaatggtt ctgtagggca tgaaaaaggc cgatggactt ttcaccgcgt
     1021 ttcatcgtat aatatatggt ctttaaatct gggggtccgg ttaggtatcg aagagcaccg
     1081 tcctcaggcc gcgcgccacc cccggaattt aagtagccgt tatctcgcca gttcaaggca
     1141 gcgccaattc cgagttttgt tcggacaaac cgttaacaga gaaaggagga ggtatgaagt
     1201 taaagccgtt gggtgatcgg gtagtggtca aaccgctgga agccgccgag aagaccaaga
     1261 gcggcatcgt cctgcccgac accgccaagg agaagcccca gcagggtaag gtggaagcgg
     1321 tcggcaaggg acgccagacc gatgatggca aggtcctgcc catggaagtc aaggtcggca
     1381 acacgattct ctacgggaag tattccggaa ccgaggtcac catcgacgac gaagagtatg
     1441 tcgtcctcca cgaaagcgac attctggcca tcgtggaata attctctcta ctggaggtga
     1501 ggaaacatgg cgggtaaaca gttaacgttc gagcaggaag ccagacagct tgtcttgaaa
     1561 ggggttcaga cgctccgaaa cgcggtggcg ccgaccctcg gcccccgggg ccgcttcgtg
     1621 atcctggaga agaagttcgg cagcccgacg gtcatcaacg acggcgtcac catcgccaag
     1681 gaagtggaac tctccaatcc ctttgagaac atgggcgccc agctggcccg cgagatcgcc
     1741 agcaagacca acgacgtggc cggcgacggc accaccacca gcgtcatcct ggctcacgcc
     1801 atcgtgagcg agggcatgaa gtacatggcc gccggcagca actcggtcca gctgcgccac
     1861 ggaatcgaga aggcggttga atcggtggtg gccaacctga agaagatgag ccagccgatc
     1921 aagggcaagg aggagatcga acaggtggcg accgtggccg cggccaatga ccacgagatc
     1981 ggcaagatca tcgccgacgc catggacaag gtgggcaagg acggcgtcat caccgtcgaa
     2041 gaggccaagg gcaccgagac caccctgaaa gtcgtcgagg gcatgcagtt cgaccgtggc
     2101 tacctctccc cctacttcat caccgacacc gaaaggatgg aagcggtcct cgaggatccc
     2161 ttcattctca tctacgacaa gaagatcagc gcgatcaagg atatcctgcc cctcctggag
     2221 aagaccgccc aggtcggcaa gccccttctg atcgtggccg aggaggtcga gggcgaggcg
     2281 ctggccaccc tggtggtcaa caagatccgc ggcaccctgc tcacggtggc ggtgaaagcg
     2341 cccgggttcg gcgaccggcg caaggcgatg ctcgacgaca tcgccgtcct gaccggcggc
     2401 aaggccatct ccgaggatat cggcctcaag ctggagaatg ttgacctggc gatgctggga
     2461 cgggccaagc gggtggtggt tgacaaggag aacaccacca tcgtcgaggg cgccggcaag
     2521 gcctccgaca tcaccggccg catcaaccag atcaagaagg aaatcgagaa gacc
//
LOCUS       DAOQYN010000237         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_45277_length_2538_cov_3.434152, whole genome shotgun sequence.
ACCESSION   DAOQYN010000237 DAOQYN010000000
VERSION     DAOQYN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45277_length_2538_cov_3.434152"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="ribB"
                     /locus_tag="PKN80_08220"
     CDS             complement(<1..677)
                     /gene="ribB"
                     /locus_tag="PKN80_08220"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNQ36031.1"
                     /translation="MNRLKEVIAQIQAGRPVIMVDDENRENEGDLVLAAEKVTPKAVN
                     FMATAARGLICVAMEGTDLDRLELKPMVPESRALHGTSFTVSVDARENVTTGISAADR
                     ATTIRTLINPRTRPEDLAVPGHIFPLRGREGGALVRAGHTEAGIDLMKIAGCHPAAVI
                     CEIMAEDGRMARRPELLKLARLNRLKLVSVAEIIAYRRQKEKLVSRELEVDLPTSYGD
                     FKLHLYRS"
     gene            complement(674..1225)
                     /locus_tag="PKN80_08225"
     CDS             complement(674..1225)
                     /locus_tag="PKN80_08225"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009747401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNQ36032.1"
                     /translation="MFTGIIQDLGRVERIAAGRLRLRTALAGLEAGESVAVNGVCLTV
                     TGTGAAAEFDLSPETRRRTALGRLRPGDRVNLERALRLSDRLGGHLVTGHVDGVARIL
                     TVRRTGTGRELSVAFPGRLRPYLAVKGSVALDGVSLTIAGLTGAKLTVALIPFTLEQT
                     TLGGKRPGETLNIEVDALARYLK"
     gene            complement(1305..1505)
                     /locus_tag="PKN80_08230"
     CDS             complement(1305..1505)
                     /locus_tag="PKN80_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36033.1"
                     /translation="MVMRIKPSRNEEEFVRGAKLEHKESDATTLYLLRLPRDLRVKIK
                     VKAAEAGQSMSDLICEILDRNV"
     gene            complement(1663..2289)
                     /gene="parA"
                     /locus_tag="PKN80_08235"
     CDS             complement(1663..2289)
                     /gene="parA"
                     /locus_tag="PKN80_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HNQ36034.1"
                     /translation="MIITVGNEKGGVGKSTIACNLAVEAVRHRRRTLLIDADSQQSAM
                     DFRAVRSENAGLPQFQAVGITKNTIHKDIQGFRGYDLIIIDSGGRDTQVFRSAILASD
                     LFIIPVLTSQYDVWATQGTTRVLEEARSFKDIEARLVINQLIPNTIVAREASDLLEGL
                     GVSLLKTRLHARVAYKQAIAEGKGVSEYEPGGKAAREIQALWREVGLK"
BASE COUNT          379 a          851 c          814 g          494 t
ORIGIN      
        1 gatcggtaaa ggtgcagctt gaaatcaccg taggaggtcg gcaggtccac ctccagttcc
       61 cggctgacca gcttctcctt ctgccgccgg taggcgatga tttcggccac cgagaccagc
      121 ttcagccgat tgagcctggc cagtttcagc agttccggcc gccgggccat ccggccgtcc
      181 tcggccatga tctcgcagat caccgccgcc ggatggcagc cggcgatctt catcaggtcg
      241 atgcccgctt cggtatggcc ggcccggacc agcgcgccgc cctcgcgccc ccgcagcgga
      301 aagatgtggc cgggcacggc caggtcctcc ggccgcgtcc gggggttgat cagggtccgg
      361 atggtggtgg cccggtcggc ggccgagatg ccggtggtca cgttctcgcg ggcgtccacc
      421 gagacggtga aggaggtgcc gtgcagggcc cggctctccg ggaccatcgg cttcagttcc
      481 agccggtcca ggtcggttcc ctccatggcc acgcagatca ggccgcgggc ggccgtggcc
      541 atgaaattga ccgctttcgg ggtgaccttc tcggcggcca gcaccaggtc cccctcgttc
      601 tcccggtttt cgtcgtcgac catgatcacc ggccggccgg cctggatctg ggcgatcact
      661 tccttcagtc ggttcatttc aggtaacggg ccagggcgtc cacttcgatg ttcagcgttt
      721 cgccgggccg cttgccgccc agcgtggtct gctccagggt aaagggtatc agggccacgg
      781 tcagtttcgc gccggtcagc ccggcgatgg tcaggctcac cccgtccagg gccaccgagc
      841 ccttgacggc caggtaaggg cgcagccggc cgggaaaggc cacgctcagc tcccggccgg
      901 ttcccgtccg gcgcacggtc agtatccggg ccaccccgtc cacgtggccg gtgaccaggt
      961 ggccgcccag ccggtcgctc agccgcagcg cccgttccag gttcacccgg tccccgggcc
     1021 ggagccggcc cagggcggtc cggcgccggg tctccgggga gaggtcgaac tcggccgccg
     1081 cgccggttcc ggtcacggtc aggcagacgc cgttgaccgc aacgctctcc ccggcttcca
     1141 ggccggccag ggccgtccgg agccggagcc ggccggcggc gatccgctcc acccggccga
     1201 gatcctggat tattccggta aacatgggat tgaggggttc ggggtgcgct ttacgggaac
     1261 cgccataatg ttagcacagc cggccgtccc tttccagccc ggcttcagac attccggtcc
     1321 aggatttcgc agatcaggtc cgacatgctc tggcccgcct cggccgcctt gaccttgatc
     1381 ttgacccgca ggtcgcgcgg cagccgcagc aggtaaaggg tggtggcgtc gctctccttg
     1441 tgctccagct tggcgccgcg gacgaactcc tcctcattgc gggacggctt gatacgcatg
     1501 accattggct tctccttgtc gattggcgat tattcgcggc ggccgcccgg gttgctttcg
     1561 gccggtcgtg gattatctcc attatttata taacaaagcc ggccgcttgt aaagggattt
     1621 tacttatgat tataatcata agcaaaataa ttattataaa tattatttaa gtccgacctc
     1681 gcgccagagc gcctggatct cgcgcgccgc cttgccgccc ggctcgtact cgctgactcc
     1741 cttgccctcg gcgatggcct gcttgtaggc cacccgggcg tgcagccggg ttttgagcag
     1801 gctgaccccc agcccttcca gcaggtcgga ggcctcgcgg gcgacgatgg tgttgggaat
     1861 gagctggttg atgaccagcc gggcctcgat gtccttgaaa ctccgggcct cctccagcac
     1921 ccgggtggtg ccctgggtgg cccagacgtc gtactggctg gtgagcaccg ggatgatgaa
     1981 gaggtccgaa gccaggatgg cgctccggaa gacctgggta tcccggccgc cggaatcgat
     2041 gatgatcagg tcgtagccgc ggaacccctg gatgtccttg tgaatggtgt tcttggtgat
     2101 gccgaccgcc tggaactgcg gcagccccgc gttttccgag cgcaccgccc ggaagtccat
     2161 ggcgctctgc tggctgtcgg cgtcgatgag cagcgtccgg cgccggtggc ggaccgcctc
     2221 caccgccagg ttgcaggcga tggtcgattt gcccacccca cccttctcgt tgcctaccgt
     2281 gatgatcatg cgcgcctccc tcgatggtga aagtctgacg tcggtcccgc ccgccccggc
     2341 tccgcgtccg cggtcccggg gtgggcctaa ataataatta taatcattat aatgataact
     2401 aaaggtcgaa aaccgccgcc ggccttgatg cgcttgtttt tttaacgggt tccgatttgc
     2461 ggaccgccgg cccggtccag caccagcagc cggtcttcgg ccagccgctt cattccggtc
     2521 aaccggaatc gggccggg
//
LOCUS       DAOQYN010000238         2537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_45308_length_2537_cov_4.626108, whole genome shotgun sequence.
ACCESSION   DAOQYN010000238 DAOQYN010000000
VERSION     DAOQYN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45308_length_2537_cov_4.626108"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKN80_08240"
     CDS             <1..470
                     /locus_tag="PKN80_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ36035.1"
                     /translation="AIGTAEIVHAANRGENLTTVFVNNAVYGMTRGQMAPTTMLGQKT
                     TTTAAGRDPRQHGYPLRVCEMLAGLDGVVYLERLALSDPASVRRIKAAIRKGFECQLS
                     GQGFSLIEVLSPCPVNWNLSPVDSLTYVREVMSPVFPPGVIKDRTCQKPAPGV"
     gene            610..1095
                     /locus_tag="PKN80_08245"
     CDS             610..1095
                     /locus_tag="PKN80_08245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNQ36036.1"
                     /translation="MDLNLLGLAISILGGALAVGLAGGGSAIGVGLAGQAASGVLAED
                     PDKFGSTLLLVALPGTQGIYGFLVGFLVIMKLGLLGSQIIFPSLFQGLQILGACLPIA
                     LAGFISAVHQGKVCAAGVGVAAKNPAAGMKALVYGALVETYAILGLVASIFFLQGIKL
                     Q"
     gene            1101..1691
                     /locus_tag="PKN80_08250"
     CDS             1101..1691
                     /locus_tag="PKN80_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HNQ36037.1"
                     /translation="MALENLLETLLDESRRQAESIRRAAETEARRLESELRDGVRRRV
                     AEQVESARRRFERDLEQAVSAGRLESRRTLLADKERFLRLVYQEGLKVLRSDPRRLAE
                     FFSLQLAGLEPGDYRVEAGRDLEASLGADWFQAAAARLRPAGIRLEFSGYRDQAGLEL
                     RLQSGHAEILISPERALELQWPELEIRLAAILFPNG"
     gene            1695..>2537
                     /locus_tag="PKN80_08255"
     CDS             1695..>2537
                     /locus_tag="PKN80_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36038.1"
                     /translation="MALFAYAVGRVRALEARLIGAERFRQLEESAGWEGLLPELAGLG
                     YPVPASDRNLSEWLRELRAGLWKLSDHLLEGTDYPYFYRLPIDFNNLVLLARSRAGLM
                     DSGFEAEPGGSLETKSLESVWSGQGWFRLPAELAAGLKEGQRRLENDGPDGFEYELAG
                     AVTRLMLRASGSSELLARLAVFFIDGWNLRSYAAGGTLRLPGGRIAPDRFGADRRELL
                     NAFSGCYPAFAAFGRDREWPDGAELEAVLDRTAAGLLEASRFVTLGPEPVLAYHYQKC
                     SEITR"
BASE COUNT          382 a          815 c          858 g          482 t
ORIGIN      
        1 cggcgatcgg caccgccgag atcgtgcacg cggccaaccg gggcgagaac ctgaccaccg
       61 tcttcgtcaa taacgccgtt tacgggatga cccggggcca gatggctccc accaccatgc
      121 tcggccagaa gaccaccacc accgcggccg gccgcgaccc ccgccaacac ggctatcccc
      181 tgcgggtttg cgaaatgctg gccggtctcg acggagtggt ttacctggaa cgactggccc
      241 tgagcgaccc ggcctcggtt cggcggatca aggccgccat ccgcaaggga ttcgaatgcc
      301 agctttccgg ccagggtttc tcgcttatcg aggttctttc gccctgcccg gttaactgga
      361 atctctcgcc ggtggattcg ctgacttatg tccgcgaggt tatgagcccg gttttcccgc
      421 ccggcgtgat caaggaccgg acttgtcaaa aaccggctcc cggcgtataa tttttctatt
      481 gtagtcctat atatatcagt gccgggtaac ccggaatccg ccgacggccc ggttgagccg
      541 ggacaaaaat cctcgtctct ccctccgggg ccgccaagtt gttttcccaa agagcaaagg
      601 aggcgttcca tggatttgaa cctgctgggt ttggccatct ccattcttgg cggcgcgctg
      661 gcggttggac tggccggcgg cggatcggcc atcggggtcg ggctggccgg ccaggcggcc
      721 agcggcgtcc tggccgagga cccggacaag ttcgggtcga cgctgctgct ggtggccctg
      781 cccgggaccc agggcatcta cgggttcctg gtcgggttcc tggtgatcat gaaactgggc
      841 ctcctgggaa gccagatcat cttcccctcc ctgttccagg ggctgcagat cctgggggcc
      901 tgcctgccga tcgccctggc cgggtttatc tcggccgtac accagggcaa ggtctgcgcc
      961 gccggcgtgg gcgtggcggc caagaacccg gccgccggga tgaaggcgct ggtttacggc
     1021 gccctggttg agacctacgc catcctgggc ctggtggcct ccatcttctt cctgcaggga
     1081 atcaaacttc agtaatcccg atggccctgg aaaacctgct tgagacgctc cttgacgaat
     1141 cccggcgcca ggccgagagc atccggcgcg ccgccgaaac ggaagcgcgc cggctggagt
     1201 cggaacttcg ggatggggtg cggcgccgcg tggcggagca ggttgaatcg gcccggcggc
     1261 ggttcgagcg tgacctggaa caggcggtca gcgccgggcg cctggaatcg aggcggaccc
     1321 tgctggccga caaggaacgc tttcttcgat tggtctacca ggagggcctg aaggttctcc
     1381 gttccgaccc ccggcgcctc gccgagtttt tttcactgca actggccggc cttgaaccgg
     1441 gcgactaccg ggtcgaagcc ggccgggatc tggaagcctc ccttggtgcc gactggttcc
     1501 aggccgccgc ggcccggctg cgtccggccg ggatccggct ggaattctcc ggctaccggg
     1561 accaggccgg cctggaactc cggctgcagt ccggccacgc cgagatcctg atttctccgg
     1621 aacgggccct ggaactgcag tggccggaac tggagatccg actggccgcc atcctttttc
     1681 cgaacggttg aaccttggca ctctttgctt acgcggtcgg ccgggtgcgg gccctggagg
     1741 cccggttgat aggggccgag cggttccgtc agcttgagga gtcggccggt tgggagggac
     1801 tccttccgga actggccggc ctgggttacc cggttcccgc ctccgaccgg aacctctccg
     1861 aatggctccg ggagctgcgg gccggcttgt ggaaactctc cgaccacctc ctggaaggga
     1921 ccgattatcc gtatttttat cggctgccga tagatttcaa caatctggtc ctgttggctc
     1981 ggagccgggc gggactgatg gattccggct tcgaagcgga accgggcggc tccctggaga
     2041 caaagtcgct ggagagcgtc tggtctggcc agggctggtt ccgcctgccg gccgagctgg
     2101 ccgccggttt gaaggaaggg cagcgccggc ttgagaatga cggcccggac ggttttgaat
     2161 acgaactggc cggtgcggtc acccgcctga tgctgagggc ctccggaagc agcgagttgc
     2221 tggcgcggct ggcggtcttt ttcatcgatg gctggaacct gcgctcctac gccgccggcg
     2281 ggacactccg gctaccgggc ggccggatcg ctccggatcg tttcggcgcc gaccgccggg
     2341 agttgctgaa cgccttttcc ggttgctacc cggccttcgc cgccttcggc cgcgaccggg
     2401 agtggccgga cggtgcggag ctggaggcgg tcctggaccg gacggccgcc gggctgctgg
     2461 aagcgagtcg tttcgtgacg ctgggtcccg aaccggtcct ggcctaccac tatcagaagt
     2521 gttccgagat cacccgg
//
LOCUS       DAOQYN010000239         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_45492_length_2531_cov_4.250808, whole genome shotgun sequence.
ACCESSION   DAOQYN010000239 DAOQYN010000000
VERSION     DAOQYN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45492_length_2531_cov_4.250808"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PKN80_08260"
     CDS             <1..852
                     /locus_tag="PKN80_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36039.1"
                     /translation="LQGREITLERIEARIARYGETGSPCPWEIGAAELAAGRQSALDR
                     FRSFGPAAVIAAGVLDGINPCAFTTIVFLVSYLAFIGRRRREILLAGVSFTLAVFLTY
                     FLVGCGLFAFLRRLPVRSLVSVLLTAAVGGLALGLGVLSFLDFLKARRGDLRGIVLQL
                     PDRVKGRIRLTISRQLRRRGLPAAAFAAGFAVSVLELACTGQVYLPTITLIAGREGLR
                     GRGLALLLLYNAAFILPLAAVFMAVYFGAASERLAGFARRRVAAVKFFTAILFFALGA
                     LLLATLL"
     gene            1029..2288
                     /locus_tag="PKN80_08265"
     CDS             1029..2288
                     /locus_tag="PKN80_08265"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNQ36040.1"
                     /translation="MDELEKEIGERVRAARAAGRQLAVAEAGSRNRCLVELRKLLAAA
                     GGEVLAANRRDVEAARAAGRNQAYLDRLELTGARLERLAGSLDEVAALPDPLNQVRWE
                     TTRPNGLRVRQVSTPIGVIAIIYESRPDVTIEAAALCLKSGNAVVLKGGSDSLDSNEV
                     FEALLHRALRAAGLPPEAVYLVKSREHRAVDFLLSRTEDLDLVIPRGGEGLIRTVVEK
                     SRVPVIKHYKGVCHTYVDRAADLEMAWTVTLNAKAQRPATCNATETLLVHREIAGSFL
                     PEMGRRFAAGGVRMRGCPETLRLVPGAEPAREADWGFEFLDLVIAIRVVASLEEAIAW
                     VNRYGTGHSEAIITADPAAAERFLAEVDAAAVYHNASTRFTDGFEFGLGAEIGISTDR
                     IHARGPMGLRELTSYKYLVYGSGQLRS"
     gene            2308..>2531
                     /locus_tag="PKN80_08270"
     CDS             2308..>2531
                     /locus_tag="PKN80_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNQ36041.1"
                     /translation="MKIGLLGGTFDPIHIGHLIIAERMREALALDRVIFIPAGRPPHR
                     PVPAASAPERLEMVRLAIAGNEAFTFSEIEL"
BASE COUNT          344 a          851 c          899 g          437 t
ORIGIN      
        1 ctccaggggc gggagatcac cctcgagagg atcgaggcgc ggatcgcccg gtacggagaa
       61 accggcagtc cctgcccctg ggagatcggg gcggcggaac tggccgccgg ccggcagtcg
      121 gcgctggacc ggtttcgctc cttcggcccg gcggcggtga tagccgctgg cgtcctggac
      181 gggatcaacc cctgtgcctt caccaccatc gtcttcctgg tctcctacct ggccttcatc
      241 ggccgccggc gccgggagat actactggcc ggggtctcct tcacgctggc ggtctttctg
      301 acctatttcc tggtcggctg cggcctcttc gccttcctgc gccggctgcc ggtccgctcc
      361 ctggtctcgg tcctgctgac ggcggccgtc ggcggactgg cgcttggcct gggcgtcttg
      421 agttttctgg attttctcaa ggcgcggcgg ggcgatcttc gggggatcgt actccagctg
      481 ccggaccggg tcaagggccg gatccggctg acgatcagcc ggcagctgcg ccgccggggc
      541 ctgccggcgg cggccttcgc ggccggcttc gcggtctcgg tcctggagct ggcctgcacg
      601 ggccaggttt acctgccgac gatcaccctg atcgccggcc gggaggggct gcgggggcgg
      661 ggcctggccc tgttgctgct ttacaacgcg gcgttcatcc tgccgctggc ggctgttttc
      721 atggccgttt atttcggggc cgcctccgag cggctggccg ggttcgcccg gcgccgcgtg
      781 gccgccgtca agttcttcac cgccatcctc tttttcgccc tgggggcgct gctgctggcg
      841 acgctgcttt aacggatgcc gcctggccgg ttcattcttg ccaggcctcc cgcctttctc
      901 agaacggctt atctaaacgg ccggattgat tgaccggagg cggcggttaa aagtaaaata
      961 cttagatatt cgagagatgg gtttaaaccg gcctggcggg aaccgggagc gttgatttcc
     1021 gcccgattat ggacgaactg gaaaaggaga tcggggaacg ggtccgggcc gcccgggcgg
     1081 ccggccggca gctggccgtg gccgaggccg ggagccggaa ccgctgcctg gttgaactgc
     1141 ggaaactcct ggcggccgcc ggcggcgagg tcctggcggc caaccggcgg gatgtcgagg
     1201 cggcccgggc ggccggccgg aaccaggcct acctggaccg gctggagctg accggggcgc
     1261 gcctggaacg cctggccggc tccctggacg aggtggccgc gctgccggac ccgctcaacc
     1321 aggtgcgctg ggagacgacc cggcccaatg gcctgcgcgt ccgccaggtc tccacgccga
     1381 tcggggtaat tgccatcatc tacgagtcgc ggccggacgt gaccatcgag gccgccgccc
     1441 tctgcctgaa gagcggcaac gcggtggtcc tcaagggcgg ctcggattcg ctcgattcca
     1501 acgaggtctt cgaggcgctg ctgcaccggg cgctccgggc ggccggcctg ccgccggagg
     1561 cggtctacct ggtcaagagc cgggagcacc gggcggtgga tttcctgctt tcgcggaccg
     1621 aggacctgga cctggtcata ccccggggcg gcgaagggct gatccggacg gtggtcgaga
     1681 agagccgggt gccggtgatc aagcattaca agggcgtctg ccacacctat gtcgaccggg
     1741 ccgccgacct ggagatggcc tggacggtca ccctcaacgc caaggcccag cggccggcca
     1801 cctgcaacgc caccgagacg ctgctggtgc accgggagat cgccggaagc ttcctgccgg
     1861 agatggggcg ccggttcgcg gccggcggcg tccggatgcg cggctgtccc gaaaccctcc
     1921 gcctcgtccc cggggccgag ccggcccggg aggcggactg gggcttcgag ttcctggacc
     1981 tggtcattgc catccgggtg gtcgcttcgc tggaggaggc gatcgcctgg gtcaaccgtt
     2041 acggcaccgg ccattccgag gcgatcatca ccgccgatcc ggccgcggcc gaacgcttcc
     2101 tggccgaggt ggacgcggcc gcggtttacc ataacgcctc cacccgtttc accgacgggt
     2161 tcgagttcgg gctgggcgcc gagatcggca tcagcaccga ccggatccat gcccggggcc
     2221 cgatggggct tcgggagctg acctcctaca agtacctggt ttacgggtcc ggccagctcc
     2281 ggagttgaaa gggagccctt taaaaacatg aagatcggct tgctgggcgg gaccttcgac
     2341 ccgatccaca tcggccacct gatcatcgcc gagcggatgc gggaggcgct ggccctggac
     2401 cgggttattt tcatcccggc cggccggccg ccgcaccgtc cggtaccggc cgcctcggcc
     2461 ccggaacggc tggagatggt ccggctggcc atcgccggca acgaggcctt caccttttcg
     2521 gagatagaac t
//
LOCUS       DAOQYN010000240         2503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_46272_length_2503_cov_4.329657, whole genome shotgun sequence.
ACCESSION   DAOQYN010000240 DAOQYN010000000
VERSION     DAOQYN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46272_length_2503_cov_4.329657"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PKN80_08275"
     CDS             <1..558
                     /locus_tag="PKN80_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ36042.1"
                     /translation="GFTLIELLVVISIIAILAAMLLPALSKAREKARQANCTNNLKQL
                     GIATRIYAEDWGGYYPYNYGNDPYFKTVLLPYLNNKTKLTYCPKTNVPDQYKYGPNMI
                     LALGPGSRTVKKISNEESPSERIYLAETTSTYVGSNNQPNNKVDYRHNGMANFLFMDG
                     HVEAAGPDKYGYLCGPAYAGIPPHW"
     gene            755..>2503
                     /locus_tag="PKN80_08280"
     CDS             755..>2503
                     /locus_tag="PKN80_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36043.1"
                     /translation="MRRFFKTLFILSMAGRLAVSNAFGQQPPRVIFHAPFEGNTTGYS
                     ENFAFPETFLGPFRPWGLGFGKGLVGQAVQLKPAAFDKGLRYPINRLFDPNRGTLSLW
                     LKLAADSNSLVMPGDKKNSIALAGLADKRIRDRLQDRQWHHFVYTWDLAGRSRKQYLD
                     GKLLAAGNYPAPFQANMISFMAGYTNLPTAYGKEELTGDYPADNWLDELLLLDQPLDE
                     AAVAAAHEAYRKGTQPFAPRPAGRQPFLPLDLTAAPAGRPSRPVGVNWELGDPAFQND
                     RRTQYRLGGYWRFQPAEFGQAAPLEDNWLYLPVPGRWLPKPARPLLLDQNFKPAVNYK
                     DRELTEYAGFFLEREFTPPAEWQGKQVRLRVAGSHINSESDFTDVYLNGRRLGTVLFQ
                     WHLFPGDRWFDLTDFIQPGRTNRITLLQGLRRPKVSDAALLAEPRLEVLETNLVSLDQ
                     LELVTIPRVSEGSLRVRIPAVNRGGRGLDLKVKALIYDREGKTAVTELGPAPLALAKG
                     FSGEAEMEFPVRDLKTWTPESPNLYQLVLEVSQAGKVIDRLPPVRFGYREIRVEKGEI
                     LLNGIPLMVSGKSHGGL"
BASE COUNT          504 a          873 c          741 g          385 t
ORIGIN      
        1 ggcttcaccc tgatcgagct gctggtggtc atcagcatca tcgccatcct ggcggccatg
       61 ctgctgccgg cactctccaa ggcgcgcgag aaggcccggc aggccaactg caccaacaac
      121 ctgaagcagc tgggcatcgc cacccggatc tacgcggaag actggggcgg ctactaccct
      181 tacaattacg gcaacgaccc ttacttcaag acggtgctcc tgccttacct gaacaacaaa
      241 accaagctga cctactgccc gaagaccaac gtcccggacc agtacaagta cggaccgaac
      301 atgatcctgg ccctcggtcc cggaagccgt accgtcaaaa agatcagcaa cgaggagagc
      361 ccgtccgaaa ggatttacct ggccgagacg accagcacct acgtcggatc caacaaccag
      421 cccaacaaca aggtcgatta ccggcacaac ggcatggcca acttcctctt catggacggg
      481 cacgtggaag cggccggacc cgacaagtac ggctacctct gcggtccggc ctacgccggg
      541 ataccgcccc actggtgaga gcagggcggc gcggagtata gagaacagcc ggcggcggga
      601 gaagaaccgc cgccggctgt cttcccaaaa cattccaaac tgaaccggcc gccaaccctt
      661 ctatcgatgt cccagaccgg acttccccca aaaccactcg gacctgacga cggcctttac
      721 ggccggtcat caaatactac cggagggaaa agttatgagg agattcttca agactctctt
      781 catcctttca atggccggcc ggctggcggt ctcaaacgcc ttcggccagc agccgccccg
      841 ggtaatcttc cacgcgccct ttgagggcaa caccaccggg tactcggaaa acttcgcctt
      901 ccctgaaacc ttcctgggcc ccttccgtcc ctggggactc ggcttcggca agggactggt
      961 cggtcaggcc gtccagctca agccggccgc cttcgacaag ggtctgcgct acccgatcaa
     1021 ccggctcttt gatccgaacc ggggcacgct ttccctctgg ctcaaactcg ccgccgactc
     1081 caactcgctg gtgatgccgg gagacaaaaa aaactccatc gccctggccg gccttgcgga
     1141 caagcggatc cgggaccggc tccaggaccg gcaatggcac catttcgtct acacctggga
     1201 cctggccggc cgcagccgga agcagtacct ggacggcaaa ctcctggccg ccgggaacta
     1261 cccggcccca ttccaggcca acatgatctc cttcatggcc ggctacacga acctgcccac
     1321 cgcttacggc aaggaggagt tgaccggcga ctacccggcc gacaactggc tggacgaact
     1381 gctcctgctg gaccagcctt tggacgaggc ggcggtcgcc gcggcccacg aggcctaccg
     1441 aaagggcacc cagcccttcg cgccccggcc ggccggccgg caacccttcc tgccgctgga
     1501 cctcacggcc gctccggccg gtcggccgtc ccgacccgta ggggtgaact gggaactggg
     1561 ggatcccgcc tttcaaaacg accggcggac ccaataccgg ctgggcggct actggcgctt
     1621 ccagccggcc gagttcggcc aggccgcgcc gctggaggac aactggcttt acctgccggt
     1681 gccgggccgc tggctgccca agccggcccg gccgctgctg ctggaccaga atttcaagcc
     1741 ggccgtcaat tacaaggacc gggagctgac cgaatacgcc ggcttcttcc tggaacgcga
     1801 gttcacgccg ccggccgaat ggcagggaaa gcaggtccgc ctccgggtgg ccgggtcgca
     1861 catcaactcc gaatcggact tcaccgacgt ctacctcaac ggccgccggc tgggcacggt
     1921 tctcttccag tggcatcttt tcccgggcga ccgctggttc gacctgaccg atttcatcca
     1981 gccgggacgg accaaccgga tcaccctgct ccagggcctg cggcggccga aggtctcgga
     2041 cgccgccctg ctggccgaac cccggctgga agtgctggaa acgaacctgg tcagcctcga
     2101 ccaacttgaa ctggtaacca tacccagggt aagcgaagga agcctgcggg tcaggattcc
     2161 ggccgtcaac cgcggcggcc gcggcctcga cctgaaggtg aaggccctga tctacgaccg
     2221 ggaaggtaag accgcggtca ccgaactggg gccggcgccg ctggcgctgg ccaaaggctt
     2281 cagcggcgaa gcggaaatgg aattcccggt ccgggatctg aagacctgga cgccggagag
     2341 cccgaacctc taccagctgg tcctggaagt cagccaggcg ggcaaggtga tcgaccgcct
     2401 cccgccggtc cgcttcggtt accgggaaat ccgggtggag aagggcgaga tcctgctcaa
     2461 cggcattccc ctgatggtca gcgggaaatc gcacggcggc ctg
//
LOCUS       DAOQYN010000241         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_46490_length_2495_cov_6.261066, whole genome shotgun sequence.
ACCESSION   DAOQYN010000241 DAOQYN010000000
VERSION     DAOQYN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46490_length_2495_cov_6.261066"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2057
                     /locus_tag="PKN80_08285"
     CDS             <1..2057
                     /locus_tag="PKN80_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36044.1"
                     /translation="GPGTMKRNLRSAGLKGSLAAAGLLAALLLARGNAGAAELLQVNG
                     AGRPVGIYCGETLFPISANLVLYPAGGERLSLYSQARLVEYARQGETRSWRGEVQTFY
                     ETLGRYRQAISPGSGGSRLLLEFTLSQAAGRRLEGAQLFLFLPAELLEGGRAGLPDRK
                     GRSVEFRLPAKNEAESAIGEGLGNSFRFEGPAGRLKLSGRLDGNRKIRLGVTRSEGQK
                     YYWLALEMAAVVEPGATARLELDLTAEAKPDRRPVRIKVNPGLAESAFQGFGGNLCFW
                     TDPEPGRRCLEEFRLGRVRLRMGLDEWEPENDNGDPERMDLEYFRRQDRPGSRLREEF
                     QLAAALHKKGIPVGWSVWKVPGWIAAGNEISGERCRIAADRWPELVESIAAYLLYARQ
                     EYGVEAEFFSFNEPDVGVQVLLSPEEHALAVNLIGGRLSKLGLKTRILVGDVYRAAET
                     IDYPATVLAKPELLPYAGGLAFHAWGGARPEEYRAWKELADRHRLPLLVTEAGTDASA
                     YLTRWYIHSFHYAVQELRLYQELLRHARPQSIMEWQYTDDYGPLQAETGTDTGPIRYR
                     PTLRYAFLKQFVNLTPAGLAVASESDRPEVLVTAFSGRPAGAGEKSGRILTIHIANLG
                     PGRTATLTGLPETIKNLKVFQSDETAFNRAVPAPVLRDGTVSLNLPPFSLTSLTDGDL
                     SP"
     gene            2158..>2495
                     /locus_tag="PKN80_08290"
     CDS             2158..>2495
                     /locus_tag="PKN80_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ36045.1"
                     /translation="MAVASKKRPLRYAIVGLGGRSAMYSSALYSSHEKYGKLAAFCDL
                     NPVRMKFWNDHFQRTHGKKPVPTYRPDRFDRMLKENRIDRLIVTSMDRTHHRYIIRAM
                     ELGCDVVTEKP"
BASE COUNT          455 a          883 c          764 g          393 t
ORIGIN      
        1 ctggtcccgg aaccatgaag agaaatctcc gctccgccgg tctcaaaggc agcctggccg
       61 cggccgggct gctggccgcg ctgctgctgg cccgggggaa cgccggcgcc gccgagctcc
      121 tgcaggtcaa cggcgccggc cggccggtgg gaatctattg cggagaaacg ctcttcccga
      181 tcagcgccaa cctggtcctc tacccggccg gcggcgaacg cctctccctt tacagccagg
      241 cccggctggt cgagtacgcc cggcaggggg aaacccggtc ctggcgcgga gaagtacaga
      301 ccttctacga aaccctgggc cgttaccgcc aggcgatttc acccggaagc ggcggttccc
      361 ggctcctcct cgaattcacc ctctcccagg ccgccggccg ccgcctggaa ggagcgcagc
      421 tgttcctttt tctgccggcc gaactcctgg agggcggccg ggccggcctg cccgaccgga
      481 aggggcgaag cgttgaattc cgactgcccg ccaaaaacga ggcggaaagc gccatcggag
      541 aaggcctcgg aaactccttc cggttcgagg ggccggcggg acgcctgaag ctgtccggcc
      601 gcctggacgg caaccgcaaa atacgcctgg gcgtcacccg ttccgaaggg cagaaatact
      661 actggctggc gctggaaatg gccgccgtcg tcgaaccggg cgccacggcc cggctggagc
      721 tggacctgac ggccgaggcc aaacccgacc gccggccggt ccggatcaag gtcaatcccg
      781 gcctggccga atcggccttc cagggcttcg gcggcaacct ctgcttctgg accgacccgg
      841 agccgggccg gcgctgcctg gaggagttcc ggctcggccg ggtacgcctc cggatgggcc
      901 tggacgagtg ggagccggag aatgacaacg gcgacccgga acggatggac ctcgaatact
      961 tccggcgcca ggaccggccc ggcagccgcc tgcgcgagga gtttcagctg gccgcggcac
     1021 tgcataaaaa aggcattccc gtcggctgga gcgtctggaa ggtccccggc tggatcgcgg
     1081 ccggcaacga gatctccggc gaacggtgcc ggatcgccgc cgaccgctgg ccggaactgg
     1141 tggaatcaat cgccgcttac ctgctctacg cccgccagga gtacggggtg gaggcggagt
     1201 tcttctcctt caacgaaccc gacgtcggcg tccaggtgct cctttcgccc gaagagcacg
     1261 ccctggcggt aaacctgatc ggcggccgcc tgtcaaaact gggcctcaag accagaattc
     1321 tggtcggcga tgtttaccgg gcggccgaaa ccattgacta cccggccacg gtcctggcta
     1381 aaccggagct gctgccctat gccggcggac tggcttttca cgcctgggga ggcgcccgac
     1441 ccgaagagta ccgggcctgg aaggaactgg ccgaccgcca tcggctcccg ctgctggtga
     1501 ccgaggccgg caccgacgcc tccgcctacc tgacccgctg gtacatccat tccttccatt
     1561 acgcggtcca ggaactgcga ctctaccagg agctgcttcg ccacgcccgg ccccagtcga
     1621 tcatggaatg gcagtacacc gacgactacg ggccgctcca ggcggaaacc ggcaccgaca
     1681 ccggtccgat ccggtaccgg ccgaccctcc gttacgcctt cctgaaacaa ttcgtcaacc
     1741 tgacgccggc cggactcgcg gtcgcttcgg aatccgaccg gcccgaagtc ctggtcaccg
     1801 ccttctccgg ccggccggcc ggggccgggg agaagtccgg acgcatcctg acgatccata
     1861 tcgccaacct cgggccgggc cggacggcca ccctgaccgg actgccggaa acaatcaaaa
     1921 acctcaaggt tttccagtcc gacgagaccg ctttcaaccg ggccgtcccg gcgcccgtcc
     1981 tgcgcgacgg caccgtcagc ctcaacctgc cgcccttctc cctgaccagc ctgaccgacg
     2041 gcgacctttc tccttgaagt ttcgcctccg tcggccccga ttccttttcc cgtgttataa
     2101 taaaatatat aatatttcaa aaagtgctcc tctctcgcca gaccggaggt ttgaaaaatg
     2161 gccgttgcca gtaaaaaaag accgctccgc tacgccatcg tagggctggg cggccgctcg
     2221 gcgatgtaca gcagcgccct ctattccagc cacgagaagt acggcaagct ggccgccttc
     2281 tgcgatctca acccggtccg gatgaaattc tggaatgacc atttccagcg gacccacggc
     2341 aagaagccgg tcccgaccta ccggccggac cgcttcgacc ggatgctcaa ggagaaccgg
     2401 atcgaccgtc tcatcgtcac ctcgatggac cggactcacc accgttacat catccgggcc
     2461 atggaactgg gctgcgacgt cgtcaccgag aaacc
//
LOCUS       DAOQYN010000242         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_46694_length_2488_cov_4.765310, whole genome shotgun sequence.
ACCESSION   DAOQYN010000242 DAOQYN010000000
VERSION     DAOQYN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46694_length_2488_cov_4.765310"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..389)
                     /locus_tag="PKN80_08295"
     tRNA            complement(318..389)
                     /locus_tag="PKN80_08295"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(355..357),aa:Ala,seq:tgc)
     gene            490..1221
                     /locus_tag="PKN80_08300"
     CDS             490..1221
                     /locus_tag="PKN80_08300"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HNQ36046.1"
                     /translation="METTKVWNLLDTGGRDGAWNMACDLKLLAAAERKEIGPTLRFYR
                     WEPPAVSLGATQKAEEEVSIEFCRREGIPVVRRPSGGGAIFHDDELTYSFVARTEAHP
                     DFGDLLKSYYLIIEGLRRGLAGLGVPADTRGGAGGGSPERYLPCFALASRHDLTSGGK
                     KLIGSAQKRRRQAFLQHGSLPFSYRPELVNRIFLRPENFFEKATCLTETLGGRRPPET
                     AVKQALQAGLESVFGCSFQGLEFKF"
     gene            complement(1243..>2488)
                     /locus_tag="PKN80_08305"
     CDS             complement(1243..>2488)
                     /locus_tag="PKN80_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNQ36047.1"
                     /translation="DLRDLGNTVVVIEHDEETIRSADYIVDLGPGAGIYGGKVVAAGS
                     LDEVLAVPESITARYLRGEERIPVPARRRRPDPGRCLEVRGAQEFNLKKIDVRFPLGL
                     LICVTGVSGSGKSTLVNEILYKGLRKRLYDTREAPGRHREIVNANLIDKVIEVDQSPI
                     GRTPRSNPATYTNLFTEIRNLFSRLPEARLRGYTPGRFSFNVKGGRCESCQGEGITKI
                     EMHFLPDIFVPCEVCRGTRYNRETLEILYKGKNIADVLKMQIQEAVEFFRNIPSVRDK
                     LAIMNEVGLGYLELGQPATTLSGGEAQRVKLAAELARKGSERTLYLLDEPTTGLHFAD
                     IRKLLHLLNRLVEKGSTVVVIEHNLDVIKTADYLIDLGPEGGERGGELVACGTPAEVA
                     RSKKSYTGRYLKPLLARLGEKE"
BASE COUNT          419 a          814 c          789 g          466 t
ORIGIN      
        1 caagtggaga taggggggct cgaaaacacc cgctaatacc aatttgcctt caaacagata
       61 gctttgttgg ctgcgtcggc cgcttcgtca gagtaccttc agtacgcttc ctggcggcct
      121 cctggccgtc gcgctctctg tccgaatgca aaccggtatc gctccaatta cgctaacctc
      181 acgcttcgct tttcaggtga ggggagaccc ctcccctgac agcccctccc ccaaaggata
      241 cctaacgcgg gggagtactc ccccgccgcc ccctgaggtc aggggttcga gccgcatcgg
      301 caagcggtga aaacaagtgg agataagggg gctcgaaccc ctaacctcct gcttgcaaag
      361 caggcgctct cccattgagc tatatcccca atcaagccaa aattttggta tgataattgt
      421 acccgcggcg gcggcgggtg tcaaagccgg tttgcccccg gccgccggtt tctaatataa
      481 tctttcgtta tggaaacgac caaggtctgg aacctgctgg ataccggcgg ccgggacggc
      541 gcctggaaca tggcctgcga cctgaagctg ctggcggcgg ccgaacggaa agagatcggc
      601 ccgaccctgc gcttttaccg ctgggagccg ccggcggtct ccctgggggc aacccagaag
      661 gccgaggagg aagtttcgat cgagttctgc cggcgggaag ggatcccggt cgtccggagg
      721 cccagcggcg gcggggccat cttccacgac gacgagctca cctactcttt cgtcgcccgg
      781 accgaggccc acccggactt tggagacctg ctgaaatcct actacctgat catcgagggg
      841 ctgcggcggg gtctggccgg actcggggtc ccggccgata cccgcggcgg cgccggcggc
      901 ggttcgcccg agcggtacct gccctgcttc gccctggcca gccggcacga tctaacctcc
      961 ggcggcaaga aactgatcgg cagcgcccag aaacgacggc gccaggcctt tctccagcac
     1021 ggatccctgc ctttcagtta ccgacccgaa ctggtaaacc gaatcttcct tcgccccgag
     1081 aactttttcg agaaggccac ctgcctcacc gaaacgctgg gcggccgacg cccgccggaa
     1141 acggccgtca agcaggcgct ccaggccgga ctggagagcg tcttcggatg ttccttccag
     1201 gggctggaat tcaaattcta accggaagtc aaccggcccg cctcactctt tttcgcccag
     1261 cctggccagc agcggcttca ggtagcggcc ggtatatgat tttttactcc gggccacctc
     1321 ggccggggtg ccgcaggcca ccagttcccc cccgcgttcc ccgccttcgg gaccaagatc
     1381 gatcaggtaa tcggccgtct tgatgacgtc caggttgtgc tcgatcacca ccacggtgct
     1441 ccccttctcc accaggcggt tcaaaaggtg gagcagtttc ctgatgtcgg cgaagtgcag
     1501 gccggtggtc ggttcgtcca gcaggtagag ggtccgttcg ctccccttgc gggccagttc
     1561 ggcggccagt ttgacccgct gggcctcccc gccggagagg gtggtggccg gctggcccag
     1621 ctccaggtag ccgaggccga cttcgttcat aatggccagc ttgtcgcgca cggaggggat
     1681 gttgcggaag aactccaccg cctcctggat ctgcatcttg agcacgtcgg cgatgttctt
     1741 gcccttgtag aggatctcga gcgtctcccg gttgtagcgg gttccccggc agacctcgca
     1801 gggcacgaaa atgtcgggca ggaagtgcat ttcgatcttg gtgatgccct cgccctggca
     1861 ggactcgcag cggccgccct tgacgttgaa gctgaaccgg ccgggcgtgt agccgcggag
     1921 gcgggcctcc ggcagccggc tgaagagatt gcggatctcg gtgaaaaggt tggtgtaggt
     1981 ggccgggttg ctccggggtg tccgcccgat gggcgactgg tccacctcga tcaccttgtc
     2041 gatcaggttg gcgttgacga tctcccggtg gcggcccggc gcctcccggg tgtcgtaaag
     2101 ccgcttccgg agtcccttgt aaaggatttc gttgaccagg gtgctcttgc cggaaccgga
     2161 gacgccggtc acgcagatca ggaggccgag cgggaaacgc acgtcgatct tcttgaggtt
     2221 gaactcctgg gcgccgcgca cctcgaggca gcggcccggg tccggccgcc ggcgccgggc
     2281 cgggaccggg atgcgctctt cgccgcgcag gtagcgggcc gtaatcgact ccggcaccgc
     2341 caggacttcg tccagggaac cggccgccac cacctttccg ccgtagatgc cggcgcccgg
     2401 tcccaggtcg acgatgtagt cggcgctgcg gatggtctcc tcgtcgtgct cgattaccac
     2461 cacggtattg cccaggtcgc gcaggtcc
//
LOCUS       DAOQYN010000243         2485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_46799_length_2485_cov_3.424280, whole genome shotgun sequence.
ACCESSION   DAOQYN010000243 DAOQYN010000000
VERSION     DAOQYN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46799_length_2485_cov_3.424280"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..464
                     /gene="yajC"
                     /locus_tag="PKN80_08310"
     CDS             150..464
                     /gene="yajC"
                     /locus_tag="PKN80_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNQ36048.1"
                     /translation="MLLQVPAVTPNAQAPAAAPNPLLSFLPLIIILFIFYFMIIMPQQ
                     KKQRKHQEMLDALKAGDQVVTIGGLHGKIVEVKVDVVILEVGTSNRLPIRVNRSAIAG
                     KA"
     gene            496..1665
                     /locus_tag="PKN80_08315"
     CDS             496..1665
                     /locus_tag="PKN80_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HNQ36049.1"
                     /translation="MRIGYFNAAAGAAGDMIAASLIDAGLPLKRLETELGKIPLTGYR
                     LEVRREERDDPRIGHPVPARRFLVRVGKHEHGRSYQEIVRLVEKSRLEAETRRLVLAI
                     YRRLAEAESVVHNGLPDHFHQIGETDSIVDIASAVIGLRALKVERLYASALNLGRPAP
                     ATALLARGLPARVNGDQPEQTTPTAAAILAELARFEAPPPMRLEAVGYGAGSRIEPEP
                     NILSFYLGEAETNPARDEVLVLETNLDDLPPNAYEPAIQALQAAGILDFILLPGLMKK
                     GRPGQKLELILPPERLEGVLEALFTHTSAFGTRLRRSERVVLEREWREVDVAGRKVRV
                     KVGRYLGREVQANPEYEDLKGLAEAEKISLARAGQLVQAAFINERPKTVKKSRKG"
     gene            1665..>2485
                     /gene="rmuC"
                     /locus_tag="PKN80_08320"
     CDS             1665..>2485
                     /gene="rmuC"
                     /locus_tag="PKN80_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNQ36050.1"
                     /translation="MPNLWFPLAGLLAGLVAGWLIGQLRAQAQRMKLDNRAVTAESAL
                     AELRGQVQQRETDLKIVQETLSEQQAGRVTAETRLAEAQLYIEEQKKFLAGAEQRFSD
                     TFNALAGESLKSNNQAFLDLAKKTLENLITEARGDLSKRQEGVEAVVKPLQETLKKYE
                     VQLRELESHRAEAYGNLNKHLEELKKAHDGLQKETGQLVSALKRPQVRGRWGEITLRR
                     VVEVAGLSRYCDFEEQVSLEGEEGKQRPDLIVRLPGNRTVVVDAKVPLAAYMDAM"
BASE COUNT          487 a          809 c          844 g          345 t
ORIGIN      
        1 acggctgggt atcccggatg attgtttctt gcgcctgagc tgaggactgg gctataattt
       61 aaaggggccg ccgccctttt tccggcgcgg ttttccggcg gccggtccta ctccggccgt
      121 caccgaccaa ccctgcacaa ggaggcacca tgctgctgca agtcccggcg gtcacaccga
      181 atgcgcaagc cccggcggcc gcgccgaatc cgctgctcag ctttctgccg ctgatcatca
      241 tcctcttcat cttctacttc atgatcatca tgccccagca gaagaagcag aggaagcacc
      301 aggagatgct ggacgccctc aaggccggcg accaggtggt gaccatcggc ggcctgcacg
      361 gaaagatcgt ggaggtcaag gtggacgtgg tgatcctgga agtgggcacc agcaaccgcc
      421 tcccgatccg ggtcaaccgc agcgccatcg ccggcaaggc ctgaaacggc cccgggccga
      481 aacgggtctc gaaagatgag aatcgggtat tttaacgccg ccgccggcgc ggccggcgac
      541 atgatcgccg ccagcctgat cgacgccggc ctgccgctca aacgactcga gacggagctc
      601 ggcaagatac cgctgaccgg ctaccgcctg gaggtccgcc gggaggaacg cgacgatccg
      661 cgcatcggcc atccggtgcc ggcccgccgt ttcctggtcc gggtcgggaa gcacgagcac
      721 ggccgctcct accaggagat cgtccggctg gtggagaaga gccggctcga ggccgagaca
      781 cgccgcctgg tgctggcgat atatcgccgg ctggccgagg ccgaaagcgt tgtccacaac
      841 ggcctgcccg accatttcca ccagatcggc gagaccgaca gcatcgtgga catcgccagc
      901 gcggtgatcg gcctccgggc cctgaaggtg gaacgcctct acgcctcggc cctcaacctg
      961 ggccggccgg cgccggccac cgccctgctg gcacgcggcc tgccggcccg cgtcaacggc
     1021 gaccagccgg aacagaccac gcctacggcc gcggccatcc tggccgaact ggcccgcttc
     1081 gaggcgccgc cgccgatgcg gctggaggcg gtcggctacg gggccggcag ccggatcgag
     1141 ccggaaccga acatcctttc cttctacctg ggcgaagcgg agacgaaccc ggcccgggac
     1201 gaggtgctgg tcctggagac caacctcgac gacctgcctc ccaacgccta tgaaccggcc
     1261 atccaggccc tccaggcggc cggcatcctc gatttcatcc tgctgccggg cctgatgaag
     1321 aagggccggc cgggacagaa actcgagctc attctgcccc cggaacggct ggaaggggtg
     1381 ctggaagccc tcttcaccca taccagcgcc ttcggaaccc ggctgcgccg gagcgaacgg
     1441 gtggtcctgg aacgggagtg gcgcgaggtg gacgtggccg gccggaaggt gcgggtgaag
     1501 gtcggccgct acctgggccg ggaggtccag gccaacccgg agtacgagga cctgaagggg
     1561 ctggccgagg ccgaaaagat atcgctggcc cgggccgggc aactggtcca ggccgccttc
     1621 attaacgaaa gaccaaaaac ggtgaagaag agccggaagg gctgatgccg aacctctggt
     1681 ttccactggc gggattgctg gccggcctgg tcgccggctg gctgatcggg cagctccggg
     1741 cccaggcgca gcggatgaaa ctggataacc gggcggtaac ggccgagagc gccctggccg
     1801 aactgcgcgg ccaggtgcag cagcgggaga cggacctgaa aattgtccag gagacgctct
     1861 ccgaacagca ggccggccgg gtgaccgccg agacacggct ggccgaggcg caactttaca
     1921 ttgaggaaca gaaaaaattc ctggccgggg ccgaacagcg tttcagcgat accttcaacg
     1981 ccctggccgg cgaatcgctc aagagcaaca accaggcctt cctggacctg gccaaaaaaa
     2041 ccctggaaaa cctgatcacc gaagcccggg gcgacctgag caagcggcag gagggagtgg
     2101 aggcggtggt caagccgctc caggagacgc tcaaaaaata cgaggtccag ctccgggagc
     2161 tggagagcca ccgtgccgag gcgtacggga acctgaacaa gcaccttgag gaactgaaga
     2221 aggcccacga cggcctccag aaggaaaccg gccagctggt ctcggccctc aagcgtccgc
     2281 aggtacgggg acgctggggc gagatcaccc tgcggcgggt cgtggaggtg gccggccttt
     2341 cgcgctactg cgatttcgaa gagcaggtct cgctggaagg cgaggagggg aagcagcggc
     2401 cggatctgat cgtccgcctg ccgggaaacc gcacggtggt ggtggacgcc aaggtgccgc
     2461 tggccgctta catggacgcc atgga
//
LOCUS       DAOQYN010000244         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_47251_length_2470_cov_3.836853, whole genome shotgun sequence.
ACCESSION   DAOQYN010000244 DAOQYN010000000
VERSION     DAOQYN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47251_length_2470_cov_3.836853"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..342
                     /locus_tag="PKN80_08325"
     CDS             58..342
                     /locus_tag="PKN80_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36051.1"
                     /translation="MRIAFIGEALAFEPFRALGHDVYPAADPESAVQVLERLDLGKYA
                     LVVLTPEVAAARNRFTGPLFLVLPGLEHRDSGQGRLLAEAIRRATGRSTI"
     gene            373..2124
                     /locus_tag="PKN80_08330"
     CDS             373..2124
                     /locus_tag="PKN80_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNQ36052.1"
                     /translation="MENGEIIKVSGPLVEAVNMKSARMYDVVYVGRQRLVGEVIRLRL
                     DRASIQVYEDTSGLGPGDPVFPSGGPLAVELGPGLLTSIFDGIQRPLDLIARETGSFI
                     RRGVNLAALPRDRRWDFTPAVRPGDKVTAGDVLGTVPETELLVHKILVPPETAGEVLE
                     IAGGSFGLDQPVGLLRTPEGQVPLKLMHTWPVRRPRPYLEKLRPEIPLVTGQRVVDTL
                     FPVAKGGSAAIPGPFGAGKTVFLHQVAKWADSQVIVYIGCGERGNEMADVLLEFPNLK
                     DPRSGRPLMDRTVLIANTSNMPVAAREASIYTGITIAEYYRDMGYDVALMADSTSRWA
                     EALREISGRMEEMPGEEGYPAYLGTRLAAFYERSGRVKCLGSRSATGSLSVVGAVSPP
                     GGDLNDPVVQMTLRAVKVFWALDDQLAYQRHFPAINWLSSYSLYLGNLEGFLGAEVAP
                     EWPELRRWAMGILGREAELKEIIRLIGTETLSLQERLLMDTARSLREDFLHQFAFDEN
                     DAYATLQKQYLMLKTIALFHQKSEEALSEGVPLNRILGLPVREQIVRNRYVAEDNLSH
                     FHALEKEIVTSFEALHN"
     gene            2143..>2470
                     /locus_tag="PKN80_08335"
     CDS             2143..>2470
                     /locus_tag="PKN80_08335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005890786.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNQ36053.1"
                     /translation="MAKEYRSVREIVGPLLLVEGVSGVTFNEIAEIILPDGSRRRGQV
                     LELDGDRALLQVFEGTTGLNPSQTRVRFEGRPLQLGVSPDLLGRVFDGLGGPIDGGPR
                     PIPGEYL"
BASE COUNT          406 a          820 c          801 g          443 t
ORIGIN      
        1 ttccgagatt acccggctgc accggctgct ccttctcaag agcaaggggt tttaggaatg
       61 aggatcgcct tcatcgggga agcgctggct tttgagcctt ttcgcgctct gggtcacgat
      121 gtttacccgg ccgccgaccc ggagtcggcg gtccaggtgc tggaacggct ggatctgggc
      181 aaatacgccc tggtggttct gaccccggag gtggcagcag cccgcaaccg gtttaccggt
      241 ccgctcttcc tggttttgcc cgggctggaa caccgcgact ccggccaggg ccgccttctg
      301 gccgaggcca tccggcgcgc caccggccgg tcgactattt aaggcggccg catcgaattc
      361 aggatccgaa aaatggaaaa cggcgagatc atcaaggttt ccggaccgct ggtggaagcg
      421 gtcaacatga aatcggcccg gatgtacgac gtggtctacg tcgggcgcca gcgcctggtg
      481 ggcgaagtca tccgcctccg cctggaccgg gcctccatcc aggtctacga ggacacttcg
      541 ggcctcggtc cgggcgaccc ggtctttccc tcgggcggtc cgctggccgt ggaactgggg
      601 ccgggcctcc ttaccagcat cttcgacggc attcagcgcc cgctggatct gattgcccgg
      661 gagaccgggt cgtttatccg gcggggcgtg aacctggcgg cgctgccccg cgaccggcgc
      721 tgggatttta ccccggcggt ccggcccgga gacaaggtta ccgccggcga cgtgctgggc
      781 acggttccgg agaccgagct gctggtgcac aagatcctgg tgccgccgga gaccgccggc
      841 gaggtgctgg aaattgcggg cggctccttc ggcctcgacc agccggtcgg gctgctgcgc
      901 accccggaag ggcaggtgcc cctgaaactc atgcatacct ggccggttcg gcggccgcgt
      961 ccctaccttg aaaagctgcg gccggagatc cccctggtca ccggccaacg ggtggttgac
     1021 acccttttcc cggttgccaa gggtggttcg gcggccatcc ccggcccttt cggcgccggt
     1081 aaaacggttt ttctgcatca ggtggccaag tgggccgaca gccaggtcat cgtttacatc
     1141 gggtgcgggg agcggggcaa cgagatggcc gacgtgctgc tggaattccc gaacctcaag
     1201 gatccccgca gcggccgtcc gctgatggac cgaacggtcc tgatcgccaa cacctcaaac
     1261 atgccggtgg cggcccgcga ggccagcatc tacaccggca tcaccatcgc cgagtattac
     1321 cgcgacatgg gttatgacgt ggccctgatg gccgactcca ccagccgctg ggccgaggcc
     1381 ctgcgcgaaa tttccggccg gatggaggaa atgccgggcg aggaagggta ccccgcttac
     1441 ctgggtaccc ggctggccgc cttctacgag cgcagcggcc gggtcaagtg cctgggcagc
     1501 cgttccgcca ccggttccct ctcggtggtc ggcgcggtct cgcccccggg cggcgacctg
     1561 aacgacccgg tggtccagat gaccctgcgg gcggtcaagg tcttctgggc cctggacgac
     1621 cagctggcct accagagaca tttcccggcc atcaactggc tctccagcta ctcactctac
     1681 ctgggcaacc tggagggttt tctcggcgcc gaggtcgccc cggagtggcc ggaactgcgc
     1741 cgctgggcga tgggcatact tggccgcgag gcggagttga aggagatcat ccggctcatc
     1801 ggcaccgaga cgctctcgct ccaggaacgc ctcctgatgg acaccgcccg gagcctgcgc
     1861 gaggatttcc tgcaccagtt cgcctttgac gagaacgacg cctacgccac gctccagaag
     1921 cagtacctga tgctcaagac catcgccctc tttcaccaga aatccgagga ggcgctttcc
     1981 gagggggtcc cgctcaaccg tatcctgggc ctgccggtcc gggaacagat cgttcgaaac
     2041 cgctacgtgg ccgaggacaa tctctcccat ttccacgcgc ttgaaaagga gatcgtcacc
     2101 tccttcgaag cacttcacaa ttgacggtga cttatgaaat cactggctaa agaatatcgc
     2161 tcggtacggg aaatcgtcgg tcccctgctc ctggtcgaag gcgtctcggg cgtcaccttc
     2221 aacgagatcg ccgagatcat cctgccggat ggttcacggc gccgcggcca ggtcctggaa
     2281 ctggacggcg accgggcgct gctgcaggta ttcgaaggca cgaccggcct caatccttcg
     2341 cagaccaggg tgcgcttcga ggggcgcccc ctccagctgg gcgtttcgcc cgacctgctg
     2401 ggccgggtct tcgacggcct gggcggcccc attgacggcg gcccgcggcc cattcccgga
     2461 gagtacctgg
//
LOCUS       DAOQYN010000245         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_47488_length_2462_cov_4.476111, whole genome shotgun sequence.
ACCESSION   DAOQYN010000245 DAOQYN010000000
VERSION     DAOQYN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47488_length_2462_cov_4.476111"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKN80_08340"
     CDS             complement(<1..292)
                     /locus_tag="PKN80_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNQ36054.1"
                     /translation="MKIRTPVCKICRREGVKLFIKGLRCHTGKCAIDKGRPAPSGHSR
                     YSSRDSNYRVQLREKEKVRAYYGVAEGQFRRYFRMAEKSRGATGEKLLEYLER"
     gene            complement(315..701)
                     /gene="rpsK"
                     /locus_tag="PKN80_08345"
     CDS             complement(315..701)
                     /gene="rpsK"
                     /locus_tag="PKN80_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNQ36055.1"
                     /translation="MARKYQKRKKKSISVPKGIAHIKATFNNTFVTITDPQGNVLVFM
                     SSGQVGFKGSRKGTPYGAGLVAREVARRAQDFGVKEVEVWVKGPGPGRETAIRSLQSA
                     GLSVTQIRDMTPIPHNGCRPPKERRV"
     gene            complement(724..1140)
                     /gene="rpsM"
                     /locus_tag="PKN80_08350"
     CDS             complement(724..1140)
                     /gene="rpsM"
                     /locus_tag="PKN80_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNQ36056.1"
                     /translation="MARIVGIEIPNNKRVEVGLTYIFGIGHSCARRILKATSISPDIR
                     VKDLTEDQINKLTQELQRNYRVEGDLKREITSSIKRLQEISAYRGVRHKRGLPVRGQR
                     THTNARTRKGPRKTVGVIRSKEQRSAVKAGDKGGKK"
     gene            complement(1148..1273)
                     /gene="rpmJ"
                     /locus_tag="PKN80_08355"
     CDS             complement(1148..1273)
                     /gene="rpmJ"
                     /locus_tag="PKN80_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNQ36057.1"
                     /translation="MKVRASVKKICPQCQIIKRRGKVRVICKNPRLGPKHKQVQG"
     gene            complement(1315..1530)
                     /gene="infA"
                     /locus_tag="PKN80_08360"
     CDS             complement(1315..1530)
                     /gene="infA"
                     /locus_tag="PKN80_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNQ36058.1"
                     /translation="MKKEEKLRVEGVVTQALPNAYFRVKLKDGHEVLAHVSGKMRMYY
                     IRILPGDKVIMEISPYDLTKARIIQRL"
     gene            complement(1527..2261)
                     /gene="map"
                     /locus_tag="PKN80_08365"
     CDS             complement(1527..2261)
                     /gene="map"
                     /locus_tag="PKN80_08365"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNQ36059.1"
                     /translation="MAHQSKAEKLRTAGRLLAVCRQELAGMVRPGVTGLDLDRRAEEI
                     ITGLGGRPAFKGYRGYPASICVSPNRVVIHGIPNSTPFREGDLVSLDLGVEKDGFYAD
                     GAVTCPVGAAAPEYRRLMETTRRALELAIARVAPGVEIDELGRAIEAEVRSNGFDVVR
                     SFCGHGIGRQLHQPPEIPNFASGNRFRLYAGLALAIEPMVNYKSGSVIILEDGWTVVT
                     ADGGYSAHFEETVLVTETGHEVVTDL"
     gene            complement(2245..>2462)
                     /locus_tag="PKN80_08370"
     CDS             complement(2245..>2462)
                     /locus_tag="PKN80_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNQ36060.1"
                     /translation="CDHCGGALYQRDDDRPDVVARRLEVYHQQSAPLREHYRRQGVML
                     TLSGEKPLEETLAAVRAALIGRNGAPE"
BASE COUNT          407 a          792 c          744 g          519 t
ORIGIN      
        1 gacgttccag gtattccagg agcttttctc cggtggcgcc ccggctcttt tcggccatcc
       61 ggaagtagcg gcggaattgt ccctcggcca ccccgtaata ggcgcgcact ttctcctttt
      121 cccggagctg gacgcggtag ttggaatccc ggctggaata acggctgtgt cccgagggag
      181 ccggccggcc cttgtcaatg gcgcacttgc cggtatggca gcgcaggccc ttgatgaaga
      241 gtttcacgcc ttcgcgacgg cagatcttgc aaaccggtgt tctgattttc atatcggact
      301 ccaaacggag ggcgtcaaac ccttctctcc ttgggcggcc ggcagccgtt gtgcggaatc
      361 ggggtcatgt cccggatctg ggtcaccgac aacccggccg actggagcga gcggatggcc
      421 gtctcgcggc ccggtcccgg acccttcacc cagacttcga cctccttgac tccgaaatcc
      481 tgggcgcggc gggcgacttc ccgggccacc agaccggctc catagggagt ccccttccgg
      541 gaacctttga aaccgacctg gccggaactc atgaagacca gcacgttccc ctgcggatcg
      601 gtgatggtga cgaaggtgtt gttgaaggtc gccttgatat gggcgattcc cttggggact
      661 gaaatcgact ttttcttgcg tttctggtat tttctggcca tggttgaccg tctccgtcag
      721 tggctatttc ttcccgccct tgtcgccggc ctttacggcg ctgcgctgct ccttgctgcg
      781 gatgacgccc accgtcttgc gcggtccctt gcgggtccgg gcgttggtat gggttcgctg
      841 cccccggacc ggaagaccgc gcttgtgccg gacgccccga taagcgctga tttcctggag
      901 gcgcttgatg ctggaggtga tttcccgctt caggtcacct tcgacgcggt aattgcgctg
      961 gagttcctgg gtcagcttgt tgatctggtc ttcggtcaga tccttgaccc ggatgtcggg
     1021 gctgatgctg gtcgccttca aaatgcgccg ggcgcagctg tgaccgatcc cgaagatgta
     1081 ggtcagcccg acctcgaccc gcttgttgtt gggaatctcg ataccaacga ttctggccat
     1141 aacgctccta accctggacc tgtttgtgct tgggaccgag ccgcgggttc ttgcagatga
     1201 cccgcacctt gccccggcgt ttaatgatct ggcactgggg gcatatcttt tttaccgacg
     1261 ctctcacttt catggctaaa tctccggaaa tttcagccgg gggaaacggc cggcctaaag
     1321 ccgctggatg atgcgggcct tggtaaggtc gtaaggggag atctccatga tcaccttgtc
     1381 acccggcagg atccggatgt aatacatgcg catcttgccg ctcacgtggg ccaggacttc
     1441 gtgaccgtcc ttgagcttga cccggaagta agcgttggga agcgcctggg tgaccacacc
     1501 ctctactctg agtttctcct ccttcttcat aaatcggtta ccacttcgtg gccggtttca
     1561 gtcaccagca cggtctcctc gaaatgggcg gaatagccgc cgtcggcggt taccaccgtc
     1621 caaccgtctt ctaaaataat taccgagcca gatttatagt ttaccatagg ctcgatggct
     1681 aacgcaagtc cggcatagag ccggaaacgg ttgccgctcg caaaattggg gatttcgggc
     1741 ggctggtgca gctgccggcc gatgccgtgg ccgcagaatg accggaccac gtcgaacccg
     1801 ttcgagcgga cctccgcctc tatcgcgcgg ccgagctcgt caatctcaac gccgggggcc
     1861 acccgggcga tggccagttc cagcgcccgc cgggtggtct ccatcaggcg ccggtactcg
     1921 ggcgccgccg cgccgaccgg acaggtgacc gccccgtccg cgtaaaaacc gtccttctcg
     1981 acgcccaggt cgagactgac caggtccccc tcccggaaag gggtcgaatt cgggatgccg
     2041 tgaatcacga cccggttggg agaaacgcag attgaggccg gataaccccg gtaacccttg
     2101 aaggccgggc gtccgcccag cccggttatg atctcttcgg cgcgccggtc caggtccaac
     2161 ccggtcaccc cgggccgcac catcccggcc agttcctgcc ggcagaccgc caggaggcgg
     2221 ccggccgtcc ggagcttttc agccttactc tggtgcgcca ttccggccga tcagcgcggc
     2281 gcgaaccgcc gccagcgtct cttccagggg cttctccccg gaaagagtca gcatgacccc
     2341 ctgccgccgg taatgttcgc gcaacggggc cgactgctgg tggtaaacct cgagacgccg
     2401 ggccacgacg tccggccggt cgtcatcgcg ctggtaaagc gccccgccgc agtgatcgca
     2461 gg
//
LOCUS       DAOQYN010000246         2460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_47568_length_2460_cov_2.617879, whole genome shotgun sequence.
ACCESSION   DAOQYN010000246 DAOQYN010000000
VERSION     DAOQYN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47568_length_2460_cov_2.617879"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /gene="infC"
                     /locus_tag="PKN80_08375"
     CDS             <1..284
                     /gene="infC"
                     /locus_tag="PKN80_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNQ36061.1"
                     /translation="ARKKQKLVEQKEIRFSPVISDHDYNFKKEHIIQFLKEGNRVKVQ
                     IRFRGRQIVHSERGKELIARLTQELAPVAAVERPSRFEGPQIDIIFRPL"
     gene            344..541
                     /gene="rpmI"
                     /locus_tag="PKN80_08380"
     CDS             344..541
                     /gene="rpmI"
                     /locus_tag="PKN80_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNQ36062.1"
                     /translation="MPKQKSNKSAAKRLRRNAGGKWKRAHAFSSHLMSSKTRKQKRQL
                     RRNGEVTAADKKQVRRMLPYA"
     gene            534..890
                     /gene="rplT"
                     /locus_tag="PKN80_08385"
     CDS             534..890
                     /gene="rplT"
                     /locus_tag="PKN80_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNQ36063.1"
                     /translation="MPRSTNNPATRARRKKVLDRAKGFRQSRSKVYQFAKEFSEKALD
                     YSWRDRRQRKRSMRSRWIIRINIAARQAGLTYRELVNGLKKAQVELNRKMLAELALKE
                     PDAFRKLAELAKQNLS"
     gene            932..2158
                     /locus_tag="PKN80_08390"
     CDS             932..2158
                     /locus_tag="PKN80_08390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNQ36064.1"
                     /translation="MKRFLSLFFSAFLLATVLFINLRYLHQPVDLLSSRILVALVDII
                     AGAALLNLLLIQDYHRSQINRIEREQQKCQLQLSDHSRESGLLSTLNEIIAIFGETPD
                     LDAILNRIAEASIRFVKADNLILQIYGSEDLSYFSRVVKGREVVELGEELLEETMLMG
                     VSTLIANTASFPRYQFLARNGFFSLLVVPLRIRGNNVGLISVLGKETNAFTGSDLEFM
                     STLAGQVSLIIENATLFEKTRRLSMTDGLTNLFNRRYFEAFLDQEIRKTESGSTPLSI
                     AMIDLDDFKKFNDHNGHLAGDEALRDIARTLRQGTKGRDIIARYGGEEFVVIFPQTLP
                     ANAGLVCENLRLAVERQTFPGEASQSGGNLTISIGLASYPDDGQTSRQVLEKADARLY
                     EAKRLGRNRVVMAGRG"
     gene            complement(2175..>2460)
                     /locus_tag="PKN80_08395"
     CDS             complement(2175..>2460)
                     /locus_tag="PKN80_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36065.1"
                     /translation="HRAEALVKEAASEKNRRLEAVRKRRPELEAAGDQEAASEIKKLA
                     GDFDARRRKQLEGVKSDLDERRADLGRRFEENRAAAFDYLLKVLTAKPEK"
BASE COUNT          537 a          766 c          686 g          471 t
ORIGIN      
        1 aagcgcgcaa gaagcagaag ctggttgagc agaaggagat ccgcttctcc ccggtgatca
       61 gtgatcacga ttacaatttc aaaaaagagc acatcatcca gttcctgaag gaggggaacc
      121 gggtcaaggt ccagatccgc ttccgcggcc ggcagattgt tcattccgaa cggggcaagg
      181 aattgatcgc ccggctgacg caggagctgg ccccggtggc ggcggtggaa agaccctccc
      241 gtttcgaagg cccgcaaatt gacataatct tccgtccgtt ataaaatata gtgtttttta
      301 atcgtaaccc tttgcggttt caacggccgg agagaataaa gcgatgccca agcaaaaaag
      361 caacaaaagc gccgccaaaa gactgcggcg caatgccggt gggaagtgga aacgggccca
      421 cgccttttcc agccacctga tgtcgtccaa aacccgcaag cagaaacgcc agctccgccg
      481 gaacggcgag gtaacggctg cggacaagaa acaagtgagg aggatgttac cctatgccta
      541 gaagcactaa caacccggcg acccgggccc ggcgcaaaaa agtcctggac cgggccaagg
      601 gttttcgcca gagccgttcc aaggtttacc agttcgccaa ggaattctcc gagaaggcgc
      661 tcgattactc ctggcgtgac cggcgccagc gcaagcgttc gatgcgcagc cgctggatca
      721 tcaggataaa cattgcggcc cggcaggccg gccttaccta ccgggagctg gtgaacggat
      781 taaagaaagc gcaggtggaa ctcaatcgca aaatgctggc ggaactggcg ctcaaggaac
      841 ccgacgcttt tcggaaactg gctgaactgg cgaagcagaa cctctcatag cgggccgacc
      901 gcggccggcc cggcgtcccc ggaagggagt aatgaagaga tttctttccc tgttcttttc
      961 cgcctttctg cttgccactg tcctcttcat caacctgcgc tatctccacc agcccgtcga
     1021 cctgctctcc agccggatac tggtggccct ggttgacatc atcgcgggag cggccctgtt
     1081 gaatctgctc ttgatccagg actatcaccg cagccagata aaccggatcg aacgcgagca
     1141 gcagaaatgc cagttgcagc tctccgacca cagccgggaa tcgggcctgc tctccaccct
     1201 caacgaaata attgcaatat tcggcgaaac tcccgacctg gatgccattc taaaccgcat
     1261 cgccgaggcc agcatccgtt tcgtcaaggc cgacaacctc atcctccaga tttacggttc
     1321 ggaagacctc tcctattttt cccgggtcgt caaggggcgc gaggtggtgg agctcggcga
     1381 ggaactcctg gaggagacca tgctcatggg tgtctccacc ctgatcgcca acaccgcctc
     1441 ctttccccgc taccaattcc tggcccgcaa cggttttttc tcgctgctgg tggtcccgct
     1501 gcgcatccgc ggcaacaatg tcgggctgat ctccgtgctc ggcaaggaga ccaacgcctt
     1561 caccggcagc gacctggaat tcatgagcac gctggccggc caggtgagcc tgatcatcga
     1621 gaatgccacc ctcttcgaga agacccgccg cctgtccatg accgacgggc tgaccaatct
     1681 tttcaaccgc cgctacttcg aagcgttcct ggaccaggaa atccggaaaa cggaaagcgg
     1741 ctcaacccct ctttcgatcg ccatgatcga cctggatgat ttcaagaagt tcaatgacca
     1801 caacggtcac ctggccgggg acgaggcctt gagggacatt gcccggactc ttcgccaggg
     1861 caccaagggg cgcgacatca tcgcccgtta cggcggggag gaattcgtgg tcatctttcc
     1921 tcagaccttg ccggccaacg ccggtttggt ctgtgagaac ctgcgcctgg ccgttgaaag
     1981 gcagaccttc ccgggcgagg catcgcaatc cggcggcaac cttaccatca gcatcgggct
     2041 ggcctcctac ccggacgacg gccagacctc caggcaggtt ctcgaaaaag ccgatgcccg
     2101 gctttatgaa gccaagcggc tgggccgcaa ccgggtggtt atggcaggca ggggataaag
     2161 gcggaaacgg acggttactt ctcaggtttg gcggtcagga ccttcagcag gtaatcgaag
     2221 gcggcggccc ggttttcctc aaaacggcgg cccaaatccg cccggcgttc atccagatcg
     2281 gatttcacac cttccaattg cttccgccgt ctggcgtcaa agtctccggc gagcttcttg
     2341 atttcagaag cggcctcctg gtcacccgcc gcttccagct ccggccggcg tttccgaacc
     2401 gcctccagcc gccggttttt ttcactggcg gcctctttca ccagggcttc agcccggtgt
//
LOCUS       DAOQYN010000247         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_47896_length_2449_cov_3.444027, whole genome shotgun sequence.
ACCESSION   DAOQYN010000247 DAOQYN010000000
VERSION     DAOQYN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47896_length_2449_cov_3.444027"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PKN80_08400"
     CDS             <1..334
                     /locus_tag="PKN80_08400"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HNQ36066.1"
                     /translation="DGQGRTGAALVNRRFNQGQGLGVYIRFPEAELPCFNQWKMIGEG
                     TYVVGLEPANAFVITRARARADGSLQFLEPGERRQYHLELGVLHSNAEIESFEGEMKG
                     LVSREPAR"
     gene            361..1353
                     /locus_tag="PKN80_08405"
     CDS             361..1353
                     /locus_tag="PKN80_08405"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ36067.1"
                     /translation="MRLLIIGGTGAFSGRITERAAAAGHRVVLFNRGQRPQPPGLNLP
                     VITGQRPALREKKAELAAFDPEGVIDSICSEPAAAEDLAALFPGVRRLVHISTVDVYG
                     QDIGGAPVDETRIPEPVSDYGKRKLACERILLEAFGDRVSIIRPSHILGRGFLTTSLW
                     SRSPYLVDRILKGRPVPAIDGGRNLMTPVYAGDAAEWTLRALERPEAGGQVFNAVGAE
                     IVTQRRYYETVAGRLGRPLRLVSVPSQVFRGRFENPPQFNWHRPYSNRKAVDHLGHAP
                     VFGLEQMLAETIDYMLANGLAEDCGRDPFDDRLTDLLLRQERELDVRLAEKAGG"
     gene            1385..>2449
                     /locus_tag="PKN80_08410"
     CDS             1385..>2449
                     /locus_tag="PKN80_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36068.1"
                     /translation="MIPRPDLLVPGAPPGTEIYQLTSEAIPSSHVYMEAQIFTPDSRR
                     FLLHRSAHAHGGGLLPHDPEHQYLLCDLEAGGARSPLITEPDAIAPSVSPDGGLVYYL
                     VDRSEADKPSLILKRIELASGDCRTLAVIDTAIAGTRYLPGRVYPLSTIRSDGRRLAA
                     ALALRDPEEPAWRWGLLVFDLENGGHRLVLEGPSWCNLHPQYCRSRQPEWSRTVLVQE
                     NHGNVHDAAGRLLQLTGGAGADIHLVNDDGSGFRDFPCGRDGREFCQGHQCWRGSGET
                     AILGTVIQSPPGEGEGRLVECRPVTAAGHRGLRSPGGWRNELSRGFPTPHFHHFGTDA
                     TGRFILTDYLPGTGNFVAWAR"
BASE COUNT          382 a          845 c          825 g          397 t
ORIGIN      
        1 cgacggccag ggccggaccg gagcggccct ggtcaaccgg cgcttcaacc aggggcaggg
       61 actgggcgtc tatatccgtt ttccggaggc ggaattgccc tgcttcaacc agtggaagat
      121 gatcggcgag ggaacttacg tggtcggcct ggaaccggcc aatgccttcg tcattacccg
      181 ggcccgggcg cgggccgacg gttcgctcca gttcctggag ccgggcgagc ggcgccagta
      241 ccacttggaa ctgggggtgc tccattccaa cgccgagatc gaatcctttg aaggcgaaat
      301 gaagggcctg gtcagccggg aacccgcccg ttaattctaa tccgcaaccg gagaaaaagc
      361 atgagactgc tgatcatcgg cggcaccggc gccttttccg gccgcattac cgagcgggcg
      421 gcggccgccg gccaccgggt cgtgctcttc aaccgggggc agcggcccca gccgcccggt
      481 ttgaacctgc cggtcatcac cggccagcgg ccggccctgc gtgagaagaa ggccgaactg
      541 gccgccttcg atccggaagg cgtgatcgat tcgatctgca gcgagccggc ggccgccgag
      601 gatctggccg ccctttttcc gggagtgcgg cggctggtgc acatcagcac cgttgacgtc
      661 tacgggcagg atatcggcgg cgccccggtc gacgagaccc ggattccgga gccggtcagc
      721 gattacggca agcgaaagct ggcctgcgaa aggatcctgc tcgaggcctt cggcgatcgg
      781 gtctccatca tccgccccag ccatatcctg ggccggggtt ttctcaccac cagcctctgg
      841 tcgcgcagcc cctacctggt tgatcgcatt ctgaaaggcc ggccggtgcc ggccatcgac
      901 ggcggccgca acctgatgac cccggtttac gccggggacg ccgccgaatg gacgctgcgg
      961 gccctggagc ggccggaggc cgggggacag gttttcaacg cggtcggcgc cgagatcgtc
     1021 acccagcgcc gctactacga aacggtggcc ggccgcctcg gccggccgct gcggctggtt
     1081 tcggtcccgt cccaggtctt ccggggccgt tttgaaaacc cgccccagtt caactggcac
     1141 cgcccgtatt ccaaccgcaa ggcggtcgac catctgggcc acgcgccggt cttcggcctg
     1201 gaacagatgc tggccgagac gattgattac atgctggcca acgggttggc cgaggactgc
     1261 ggccgggacc cgttcgacga ccggctgacc gacctgctcc tgcgccagga gcgggaactg
     1321 gacgtccggc tggccgaaaa ggccggcggg tgagaagtcg gacaattctt cacgggagac
     1381 cgagatgata ccaagaccgg acctgctggt cccgggcgcg ccgccgggga ccgagattta
     1441 ccagctgacc tccgaagcga ttccctcctc ccacgtttac atggaggccc agattttcac
     1501 gcccgattcc cggcgctttc tcctgcatcg gtccgcccac gcccacggcg gcggcctttt
     1561 accgcacgac ccggagcacc agtacctgct ctgcgacctg gaagcgggag gcgcccggtc
     1621 gcccctgatc accgagccgg acgcaatcgc tccctcggtg agcccggacg gaggcctggt
     1681 ctattacctg gtggaccgca gcgaggcgga taagcccagc ctgattctca agcggatcga
     1741 actggccagc ggcgattgcc ggaccctggc cgtcatcgat accgcgatcg ccggcacccg
     1801 gtaccttccg ggccgggtct acccgctttc caccatccgt tccgacggcc ggcggctggc
     1861 ggcggcgctg gcgctccggg acccggagga gccggcctgg cgctggggcc tgctggtctt
     1921 tgacctggag aacggcggcc accggctggt cctggagggg ccgtcctggt gcaacctcca
     1981 tccgcagtac tgccgttccc gtcaaccgga gtggtcccgg acggtgctgg tccaggagaa
     2041 ccacggcaat gttcatgatg cggccggccg cctcctgcag ttgaccggcg gcgccggcgc
     2101 cgacatccac ctggtcaatg acgacggcag cggtttccgg gactttccct gcggccgcga
     2161 cggccgggag ttctgccagg gacaccagtg ctggcgcggt tccggcgaaa cggccatcct
     2221 gggcaccgtc atccagtccc cgcccgggga gggcgagggt cgcctggttg agtgccggcc
     2281 ggttacggcg gccggccacc gcggccttcg gagtccgggc ggctggcgca acgagctgag
     2341 ccgcggtttt ccgacgccgc attttcacca tttcgggacc gacgcgaccg gccgcttcat
     2401 cctgaccgat tacctgcccg ggaccggcaa cttcgtggcc tgggcccgg
//
LOCUS       DAOQYN010000248         2440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_48172_length_2440_cov_5.020964, whole genome shotgun sequence.
ACCESSION   DAOQYN010000248 DAOQYN010000000
VERSION     DAOQYN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48172_length_2440_cov_5.020964"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /gene="yidC"
                     /locus_tag="PKN80_08415"
     CDS             <1..685
                     /gene="yidC"
                     /locus_tag="PKN80_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNQ36069.1"
                     /translation="TGNHALRSDGRLSRFIMSILTFFHRIIPNWGVAIILLTILMKAI
                     FFPLTRISLRTMKTMQKLQPILQDLSKKYRDNPQAMQQEMFGIYRQYRINPMSGCLPM
                     LIQIPIFISMFIMLRTSIVLRGAGFGLWINDLSLPDALFALPLGGRALPLNLLPLIMG
                     VTMFFQQKLSSAGATGPAAQQQKMMMVFMPVMLTVLLYNLPSGLMLYWTVTNLLSIVE
                     QEVATHHLK"
     gene            816..1742
                     /locus_tag="PKN80_08420"
     CDS             816..1742
                     /locus_tag="PKN80_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36070.1"
                     /translation="MTVKSVSFRGWKKCLKLNEGRLELIVTTEVGPRVIGLSAGGAEN
                     EFGVIEAEAGRTGGSQWRIYGGHRLWHSPERAGRTYSPDNGPIKIEQTGGRVRLVQPV
                     EAETGIEKSLEISLPPGKFLRAVVNHRLVNRGHWSVELAPWALSVLAPGGVAVVPQPN
                     RPKSDPFQPDRILSLWPYTRLDDPRLTIGRRYLMLRQDPQQAHPCKFGVNDIDNWCAY
                     ANRGRVFLKRFRYAPGAAYPDGGASVEVYTNHLFLELETLGPLVRLEPGAAVEHREEW
                     LLLEGVATPAGEREVSGKFVPALRKMLGRIGR"
     gene            complement(1752..2225)
                     /gene="rnc"
                     /locus_tag="PKN80_08425"
     CDS             complement(1752..2225)
                     /gene="rnc"
                     /locus_tag="PKN80_08425"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNQ36071.1"
                     /translation="MDLEKSGRGRADKLDRLEELLGYRFRDRELLRRALTHPSYAFEH
                     GQPSNERLEFLGDAVLSLLVAERLYRDRPEAAEGDLTQRRAELVRGTYLAVKARDLQL
                     GSFLGLGRGEEKQGGRRTPSNLANALEAVIGAIFLDGGLQSARQFIQRHFTAAAK"
     gene            complement(2228..>2440)
                     /locus_tag="PKN80_08430"
     CDS             complement(2228..>2440)
                     /locus_tag="PKN80_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36072.1"
                     /translation="WFDARGREVLRIEYNRAGLLVGEMAAGPAQPGLYQRAPYLPPMI
                     AILMLILLSGWQFCPPVRAYVLKTGL"
BASE COUNT          423 a          826 c          790 g          401 t
ORIGIN      
        1 taccggaaat catgccctcc ggtccgatgg tcgcctgagc cgcttcatca tgtcgatcct
       61 caccttcttc caccggatca ttcccaactg gggcgtggcc atcatcctgc tcaccatcct
      121 gatgaaggcc atcttcttcc cgctgacccg catcagcctg cggacgatga agacgatgca
      181 gaagctccag ccgatcctcc aggacctgtc caagaagtac cgggacaatc cccaggcgat
      241 gcagcaggag atgttcggga tctaccggca gtaccggatc aacccgatgt ccggctgcct
      301 gccgatgctg atccagatac cgattttcat ctcgatgttc atcatgctgc gcacctcgat
      361 cgtcctgcgc ggggccggtt tcggcctctg gatcaacgac ctatcgctgc cggacgcgct
      421 cttcgcgctg ccgctcggcg gccgggcgct gccgctcaac ctgctgccgc tgataatggg
      481 cgtcacgatg ttcttccagc agaagctgag ctcggccggg gccaccgggc cggccgccca
      541 gcagcagaag atgatgatgg tcttcatgcc ggtgatgctg acggtcctgc tctacaacct
      601 gccctcgggc ctgatgcttt actggacggt caccaacctg ctctccatcg tcgagcagga
      661 agtggccacc catcacctca agtaatcctt ccggccggcc ggagcggttc cagcgcccgg
      721 ccggccgggt ctttcacccc cgccccgtag tacaatatcc ttaaatcggc gcgaaacggc
      781 cgacggctgc ctaacttatc cggacagggg gaagcatgac cgtcaaaagc gtttcatttc
      841 ggggctggaa gaagtgcctc aagctcaacg agggaaggct ggaattgatc gtaaccaccg
      901 aggttgggcc gcgggtgatc ggcctctccg ccggcggcgc ggagaacgag ttcggagtga
      961 tcgaggcgga ggcggggcgc accggaggca gccagtggcg gatttacggc ggccaccggc
     1021 tctggcacag cccggaacgg gccggacgga cctactcccc ggacaatggc ccgatcaaga
     1081 tcgaacagac cggcggccgg gtgcggctgg tccagccggt cgaagccgag accgggatcg
     1141 aaaagagcct ggagatcagc ctgccgcccg ggaaatttct ccgggccgtg gtcaaccacc
     1201 ggctggtcaa ccgcggccac tggtcggtgg aactggcccc ctgggccctg tcggtgctgg
     1261 cgcccggcgg cgtggcggtg gtcccgcagc cgaaccggcc gaaatcggat cccttccaac
     1321 ccgaccggat cctgtcgctc tggccctaca cccgcctgga tgatccgcgc ctcaccatcg
     1381 gccgccggta cctgatgctc cggcaggacc cgcaacaggc ccacccctgc aagtttggcg
     1441 tcaacgacat tgataactgg tgcgcctacg ccaaccgggg ccgggtcttc ctgaagcgct
     1501 tccggtacgc gccgggagcc gcctacccgg acggcggggc ctcggtcgag gtctacacca
     1561 accacctctt cctggagctg gagacactgg ggccgctggt ccggctggaa ccgggcgccg
     1621 cggtcgaaca ccgcgaggag tggctacttc tggagggcgt ggcgacgccg gccggcgaga
     1681 gggaggtttc cgggaagttc gtcccggcgc tcaggaagat gctggggcgg atcggcaggt
     1741 agccgcccgg atcactttgc ggcggcggta aagtggcgct ggatgaactg gcgggccgac
     1801 tgaaggccgc cgtccaggaa gatggcgccg atgaccgcct ccagggcgtt ggccaggttt
     1861 gacggcgtcc ggcggccgcc ctgcttctcc tcgccccggc ccaggcccag gaaggacccc
     1921 agctgcaggt cccgcgcctt gacggccagg tatgtccccc gcaccaactc cgcccgccgc
     1981 tgggtgagat ccccctcggc cgcctcgggc cggtcccggt atagacgttc ggccaccagc
     2041 agggaaagca ccgcgtcgcc cagaaattcc agccgctcgt tggagggctg gccatgttcg
     2101 aaggcgtagg aggggtgggt cagggcccgc ctgagcagct cacggtcgcg gaaccggtac
     2161 ccgagaagct cctccagccg atccagttta tccgcccttc cccgcccgct tttctcaaga
     2221 tccatggcta caacccggtc ttcaagacgt aggcccggac cggggggcag aactgccagc
     2281 cggagagaag aatcagcatg agaatggcga tcatgggcgg caggtaggga gcacgctggt
     2341 agagtccggg ttgggccggg ccggcggcca tttcgccgac cagcaggccg gcccggttgt
     2401 attcgatgcg aagcacctcc cggccccggg cgtcgaacca
//
LOCUS       DAOQYN010000249         2395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_49605_length_2395_cov_5.176068, whole genome shotgun sequence.
ACCESSION   DAOQYN010000249 DAOQYN010000000
VERSION     DAOQYN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49605_length_2395_cov_5.176068"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2395
                     /locus_tag="PKN80_08435"
     CDS             <1..>2395
                     /locus_tag="PKN80_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36073.1"
                     /translation="IAALPSARPELPAGLDWLILKPTVGNQSRTLYELSGRWRFQPSA
                     AERNGNELVNPGLFGPEWAYANTPGNWQASGMVFDQAGKPLAAWQGKALKDFPAAWYE
                     RDFNLPARYRSGRVLLHFPRVLGMAAVYLNGRLVGRVETTAPRTFELNSFLAWDRPNR
                     LTVYNGLPFPAGMRVNGFAEAPSLEVRDTQEFDLSQPLINPSFRRSALDLLLPVENLS
                     GKPLTLSARLRILDYREDKEVFLSEPREIRLAKGFNGDQAVSFPVSGLKTWSPETPNL
                     YRCVVEISQAGKVVDLAYPEAFGYREVWVSGSDIFLNGKHLSIRGKSHNYLNGYGFNA
                     QELALLKETGQNADRTNSPQPEFEKSLTVTDEFGWLVFYNGGGDRAAIRKILGRIGNH
                     PSFIGWQYWSNGYINGPHGHPMQIGGRVDESIQKSDPDYRVMRELSQWDPYRLNAYYR
                     LGTGGQFRSIMHYIGFGTPVQNMAEWTRWWAKEQPEPLVPMELTALQITQETPLWQKG
                     SKEVILAEQAARYFGERPYRQVDEPLAATFAKPDFGINQWLLSPIRYDVKSMILERTL
                     RAWRTYGINGYLLHMDGKVDQCYTAGKLNRQGEALKRNNAPVLFYLAGPESDFVTREH
                     HFFSGRPVRKSAVVINDRLETLPGRVDWQVADASGRKLAGGGQAVTVENGGRLFVPVE
                     FTAPAVQARQELVITATLQDGAGQVLYEDSLPVQVFPEAAFTWDRNVKVMLIDSRGDT
                     SRLLEEMGVPYQVLNKDVIDRGLSSFKGYSLLIIGRDSYPEAAEIFKTWMPASGAVEE
                     "
BASE COUNT          420 a          825 c          743 g          407 t
ORIGIN      
        1 atcgcggcgc ttccctcggc ccgtcccgaa cttccggccg ggctcgactg gctgattctc
       61 aagccgaccg tcggcaacca gtcgcgcacc ctctacgagc tctccggccg ctggcgtttt
      121 cagccctcgg ccgccgagag aaacggtaac gaactggtca acccgggcct cttcgggccg
      181 gagtgggcct acgccaacac gcccggcaac tggcaggcgt ccggcatggt ctttgaccag
      241 gccggcaaac cgctggccgc ctggcagggg aaggcgctca aggatttccc ggccgcctgg
      301 tacgagcgtg acttcaacct gccggcccgc taccgctcgg ggcgggttct gcttcacttt
      361 ccgcgcgtcc tgggtatggc cgcggtctac ctcaacggcc ggctggtcgg ccgggtcgag
      421 accaccgcgc cccggacctt cgaactgaat tccttcctgg cctgggaccg gccgaaccgg
      481 ctcaccgttt acaacgggct gcccttcccg gccgggatga gggtgaacgg tttcgccgag
      541 gcgccgtccc tcgaggtgcg ggacacccag gaattcgacc tcagccagcc gttgatcaac
      601 ccctccttcc gccgtagcgc cctggacctg ctcctgcccg tggagaacct ttccggcaag
      661 ccgttgaccc tttcggcccg gctgcggata ctcgattacc gggaggataa ggaggtcttt
      721 ctatccgagc cgcgcgaaat caggctggcc aaggggttta atggcgacca ggcggtcagc
      781 ttcccggtct ccggcctcaa gacctggtcg ccggagacgc ccaaccttta ccggtgcgtg
      841 gttgaaatca gccaggccgg caaggtggtc gacctggcct atcccgaagc cttcggctac
      901 cgggaggtct gggtctccgg cagcgacatc ttcctgaacg gaaagcatct ttcgatccgc
      961 ggcaagtccc acaactacct gaacggctac gggtttaacg cccaggagct ggccctcctg
     1021 aaggagaccg gccagaacgc cgaccggacc aacagtcccc agccggagtt cgagaagagc
     1081 ctgacggtga ccgacgagtt cggctggctg gtcttctaca acggcggcgg cgaccgggcc
     1141 gccatacgca agatcctggg ccggatcggc aaccatccat cctttatcgg ctggcagtac
     1201 tggtccaacg gctacatcaa cggcccccac ggccacccga tgcagatcgg cggccgggtg
     1261 gacgagtcga ttcagaagag cgaccccgac taccgggtga tgcgcgagct gagccagtgg
     1321 gatccctacc gcctcaacgc ctactaccgc ctgggtaccg gcggccagtt ccgctccatc
     1381 atgcattaca tcggtttcgg caccccggtc cagaacatgg cggaatggac gcgctggtgg
     1441 gcgaaggagc agccggagcc cctggtgccc atggagctga ccgccctcca gatcacccag
     1501 gagacgcccc tctggcagaa ggggagcaag gaagtcatcc tggccgagca ggccgcccgg
     1561 tacttcggcg aacgccccta ccggcaggtg gacgagccgc tggccgccac cttcgccaag
     1621 cccgatttcg ggatcaacca gtggcttctt tcgccgatcc gttacgatgt caaatccatg
     1681 attctggagc ggaccctgcg cgcctggcgg acttacggca tcaacggcta cctgctccac
     1741 atggacggga aggtcgacca gtgctacacc gccggcaagc tcaaccggca gggtgaagcg
     1801 ctcaagcgca acaatgcgcc ggttctcttc tacctggccg gtccggagtc cgattttgtc
     1861 acccgggagc accacttctt ctccggccgg ccggtgcgca agagcgcggt cgtcatcaac
     1921 gaccgcttgg aaaccctgcc gggtcgggtt gactggcagg tggccgacgc ctccggccgc
     1981 aagctggccg gcggcggcca ggcggtcacg gtcgaaaacg gcggccgcct cttcgttccg
     2041 gtggagttca ccgccccggc ggtccaggcc cgccaggaac tggttatcac ggccaccctt
     2101 caggatggcg ccggccaggt cctctacgaa gacagtcttc cggttcaggt ctttccggag
     2161 gcagccttca cctgggatcg gaatgttaaa gtaatgctga tcgacagccg cggcgacacc
     2221 agccgcctgc tggaggagat gggcgttccc taccaggtcc ttaacaagga cgtcatcgac
     2281 cgcggcctct cctccttcaa gggttactcg ctgctgatca tcggccgcga cagctacccg
     2341 gaagcggccg agatcttcaa gacctggatg ccggcctccg gcgcggtcga agagg
//
LOCUS       DAOQYN010000250         2388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_49831_length_2388_cov_2.944706, whole genome shotgun sequence.
ACCESSION   DAOQYN010000250 DAOQYN010000000
VERSION     DAOQYN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49831_length_2388_cov_2.944706"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PKN80_08440"
     CDS             <1..604
                     /locus_tag="PKN80_08440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ36074.1"
                     /translation="QAPGAATAIAGDRDYLLYVGWVQERKGLHVLIEALAIAQPDLPA
                     DLKLLVVGETEQVAPSYVNQVRTLVNQLKLSKRVLFLGRRPPAETTGLISRARAVVIP
                     EQWENMSPVVLVEAMHYGRPILASRIGGIPEFIEDGRNGLLFKPDDRQELARRLVQII
                     KNPGQADTLGRNAAADAGRIWNTERNLGRLLELYRSLIHA"
     gene            516..1319
                     /locus_tag="PKN80_08445"
     CDS             516..1319
                     /locus_tag="PKN80_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNQ36075.1"
                     /translation="MRRLTPEESGTPNATSGGCSNFTGALSMPDDFFSRPLKCVVLCA
                     GRGTRLARPEYPKSMVEVAGRPLIDHLIDFWSRYTHDFIFVVGYQKDRVIDFLKKMPI
                     RASFVEQKELKGIGYALSLVEKLVSEKFIVVLGDCLCQGDFSFPAEMEQGVGVWRTGN
                     PADIHRSYSVEISDGLITKVVEKPKELVNDLCGLGFYFFDRAVFNYIRKTSPSPLRNE
                     VEITDVIEEMIMGEEHISPVFLSGNYINITYPDDLKRAAAFLKKPLLKK"
     gene            1316..>2388
                     /locus_tag="PKN80_08450"
     CDS             1316..>2388
                     /locus_tag="PKN80_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNQ36076.1"
                     /translation="MRIAIDVSPLYETNLTGIGLAIVNLLHGLARIDAYNDYRLLYIG
                     GLKSQAAERLKLVRKRLPAQSNFPFCHHRFPSRLNLNLVREYLLPVNCFTGDFDLLHV
                     PYHFAPLVRGRRTIHTVYDLTPVINPAWHTPRNAAEFRRSLRLITRRTDRVLAISEST
                     GRDLVRLFDFPADRVTVVHLGVDRERFFPLDEAQRPAARQKLADYGIKGEYLLYVGTI
                     EPRKNLERLLLAFDRLRRLGHRECRLVIAGKRGWLCDGYDKTLAGLPAATREMIIQTE
                     YFPTSDLAALYNLAALLVYPSLYEGFGLPVLEAMACGCPVVTSNVSSLPELAGGAGRL
                     VDPLDVEAIAAAISAVLADPAER"
BASE COUNT          489 a          756 c          684 g          459 t
ORIGIN      
        1 ccaggcgcca ggcgccgcaa ccgccatagc cggcgaccgc gattacctgc tctacgtcgg
       61 ctgggtccag gaaagaaaag gattgcatgt cttaatcgag gctctggcga tcgcgcaacc
      121 ggacctgccg gcggatttga aactgctggt tgtcggtgaa acggaacagg ttgcgccgtc
      181 atacgtaaac caagtccgca ccttggtaaa tcagctcaag ctttccaaac gggtgctctt
      241 tctgggccgc cggccgccgg ctgaaacaac cggtctgata agtcgcgccc gggcggtggt
      301 cataccggaa caatgggaaa acatgtcacc ggtggttttg gtggaggcca tgcattacgg
      361 acgcccgatc cttgccagcc ggatcggcgg catcccggag ttcatcgaag acggccgcaa
      421 cggattgctt tttaaacctg acgaccggca agaactggcc cggcggctgg tacaaatcat
      481 caagaacccc ggccaggccg acaccctggg cagaaatgcg gcggctgacg ccggaagaat
      541 ctggaacacc gaacgcaacc tcgggcggct gctcgaactt taccggagcc ttatccatgc
      601 ctgacgattt tttctcgcga ccgcttaaat gcgtggtgct ctgcgccgga cgaggcacgc
      661 gactggcccg tccggagtat cctaagagca tggtggaagt ggccggacgg ccattaatcg
      721 atcacctgat tgatttctgg agccgctata cccatgattt tattttcgtg gttggctacc
      781 agaaagaccg ggtcatcgac tttctgaaaa aaatgccgat cagggcctct tttgtagaac
      841 aaaaggaact caaaggcatc ggttatgcac tttcccttgt cgaaaaattg gtctcggaaa
      901 agttcattgt ggtcctgggc gattgcctct gtcagggcga cttcagcttc ccggcagaga
      961 tggagcaggg agtcggggtc tggcggaccg ggaacccggc ggatatccac cgcagttact
     1021 cggtggaaat atccgacggc ctgatcacca aggtggtgga aaaaccaaaa gaactggtca
     1081 acgatctctg tggactgggc ttctattttt tcgaccgggc ggtcttcaac tatatccgaa
     1141 aaacatcacc ttcaccattg cgcaacgaag tggaaataac cgacgtgatt gaagaaatga
     1201 tcatgggaga agaacacatc tcgccggtct ttctctccgg gaactatata aacatcacct
     1261 atccggacga cctgaaacgg gcggccgcct ttcttaaaaa accgctctta aaaaaatgag
     1321 aatcgccata gacgtttccc cgctctacga gaccaatcta accgggatcg ggctggccat
     1381 cgtcaacctg ctgcacggcc tggcccggat cgatgcctac aacgactacc ggctcctcta
     1441 catcggcggc ctgaagtcgc aggcggccga acggctgaag ctggtccgga aacggctgcc
     1501 ggcgcaatcc aatttcccct tttgccacca ccggttcccc tcgcggctca acctcaacct
     1561 ggtccgggaa tacctgcttc cggtcaactg cttcaccggc gatttcgacc tgctccacgt
     1621 cccgtaccac ttcgcgcccc tggtccgggg ccgccggacc atccacaccg tttacgacct
     1681 gaccccggtc atcaaccccg cctggcatac gccccgcaac gcggccgagt tccgccggag
     1741 cctccggctc atcacccggc gcaccgaccg ggttctggcc atctccgaaa gcaccggccg
     1801 ggacctggtc cggctcttcg actttccggc cgaccgggtg acggtggtcc acctgggcgt
     1861 ggatcgggaa cgcttctttc cgctggacga ggcgcaacgg ccggcggccc ggcaaaagct
     1921 cgctgactac ggcattaaag gtgaatacct gctttacgtg ggcaccatcg aaccgcgcaa
     1981 gaacctggaa cggcttctgc tggccttcga ccggctccgc cggctcggcc accgggagtg
     2041 ccggctggtc atcgccggca aacgcggctg gctctgcgat ggttatgaca aaaccctggc
     2101 gggactgccg gccgcgaccc gggaaatgat catccagacc gagtacttcc ccacgtcgga
     2161 cctggccgcc ctttacaacc tggccgcgct cctggtttac ccgtcgctct acgaggggtt
     2221 cggactgccg gtgctggagg ccatggcctg cggctgcccg gttgtcacct ccaacgtctc
     2281 ctcgctgccg gaactggccg gcggcgccgg ccggctggtc gacccgctcg acgtcgaggc
     2341 gatcgccgcg gccatctcgg cggtcctggc cgatccggcc gaaagaga
//
LOCUS       DAOQYN010000251         2379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_50094_length_2379_cov_4.706540, whole genome shotgun sequence.
ACCESSION   DAOQYN010000251 DAOQYN010000000
VERSION     DAOQYN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50094_length_2379_cov_4.706540"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKN80_08455"
     CDS             complement(<1..458)
                     /locus_tag="PKN80_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ36077.1"
                     /translation="MKNGARVASEKFEVIVAGGGPAGLAAAWAAGRAGARVRLLERYG
                     FLGGMATAGQIGSILGHYVTEGQPAVTGFLERLINRMASLDGTAPAWEQARRRPGISF
                     DVELFKYAADQLMLEAGVDLRFHTFACGVRTRSGRLTGLEIASKDGRGLLS"
     gene            complement(674..1750)
                     /locus_tag="PKN80_08460"
     CDS             complement(674..1750)
                     /locus_tag="PKN80_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNQ36078.1"
                     /translation="MKLKHRRGAALLLLLTGLLLARTPLFGQTAAAPAGLTPSRNAIL
                     VSWDGADRRTVRELLKESKLPNIAALAAEGSFQEIQVVGHATSTKPGHVEMLTGLDAL
                     TTGTVTNQKYRPVPEGYTVFERLEQHFGRDRIKTVFLSSKTGNVGGRSPEEATAAEVA
                     KARRKSKTITDTALARIRIPGDTFYLAKQQIDLFDARDRKAGQTGPLALKYLSDVKGN
                     RFFMFIHFADPDKEGHSFGSGSPEYVQAIIECDRWLGKFIEWLKQENLYQSTLVYVNV
                     DHGFDPNATSHSNAPDGWLATNDRQVKRSGILSDVPATIMARFGLDIPALKPRLLGAD
                     LAGPVPPDRGLRVNLLPKAGGQEY"
     gene            2107..>2379
                     /locus_tag="PKN80_08465"
     CDS             2107..>2379
                     /locus_tag="PKN80_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNQ36079.1"
                     /translation="MKILKRSIIMVAAAWGLLGASLLFGATPGSLKWTCSYGGTNRTS
                     PAVARDGTVYIGGNSEFKAINPSDGSIRWRFTVAGNEFHAPPVILPD"
BASE COUNT          375 a          712 c          825 g          467 t
ORIGIN      
        1 gataggaggc cgcggccgtc cttgctggcg atctcgaggc cggtcagccg gccggaccgg
       61 gtccggacgc cgcaggcgaa ggtgtggaag cgcaggtcga cgccggcctc cagcatcagc
      121 tggtcggccg cgtacttgaa gagttcgacg tcgaaggaaa tgccgggccg gcggcgggcc
      181 tgctcccagg ccggcgccgt cccgtcgagg ctggccatcc gattgatcag gcgttccagg
      241 aaaccggtga ccgccggctg cccttcggtg acgtagtggc cgaggatgga gccgatctgg
      301 ccggcggtgg ccatgccgcc aaggaagccg tagcgttcca acaggcggac tcgggcgccg
      361 gcccggccgg ccgcccaggc ggccgccagc ccggccgggc cgccgccggc cacgatcacc
      421 tcgaattttt cagacgcgac gcgggctccg ttcttcatgg ctttctccgg atggacgggt
      481 gaaaagaagg cgtttggctt aacggttaga atatcaaaaa ggcgggcgct tggcaagctg
      541 tcgggccggc ccccggcctc gcttaatgac tgccggcccg gaactcctcc aggctgacga
      601 aaccgtcgcg gtcggtctcc cgctgcttga tgaatttccg gcgagcctct tcggggccga
      661 tccggccgcc ggtctaatac tcctggcccc cggccttggg cagcaaattg acgcggaggc
      721 cgcggtcggg aggcaccggt ccggccaggt cggcgcccag cagccgcggt ttcagggccg
      781 gaatatccag gccgaaacgg gccatgatgg tggccgggac gtcggagagg atgccggacc
      841 tttttacctg gcggtcattg gtggccagcc agccgtcggg ggcgttggag tggctcgtgg
      901 cgttcggatc gaagccgtgg tcaacgttga catagaccag ggtcgactgg tagaggttct
      961 cctgcttgag ccactcgatg aacttgccca gccatcggtc gcactcgatg atggcctgga
     1021 catactcggg gctgccgctg ccgaaactgt gcccctcctt gtccggatcg gcgaagtgga
     1081 tgaacatgaa gaagcggttt cccttgacgt cggacaggta cttgagggcc agcggtccgg
     1141 tctgaccggc cttgcggtcg cgggcgtcga agaggtctat ctgttgtttg gccaggtaga
     1201 aggtgtcgcc ggggatcctg atcctggcca gggcggtgtc ggtgatggtt ttggacttgc
     1261 gcctggcctt ggccacctcg gcggcggtgg cctcctcggg actccggccg ccgacgttcc
     1321 cggtcttgct ggagaggaag acggtcttga tccggtcgcg gccgaaatgc tgctcgaggc
     1381 gctcgaagac ggtatatccc tcgggtaccg gccggtactt ctggttggtg acggttccgg
     1441 tggtcagggc gtccaggccg gtgagcattt cgacgtggcc gggcttggtc gaggtggcgt
     1501 ggccgaccac ctggatctcc tggaaggagc cttcggcggc cagggcggcg atattgggca
     1561 gtttcgactc cttcagcagt tcccggacgg tccggcggtc ggcgccgtcc caggagacca
     1621 ggatggcgtt gcgggagggg gtcaggccgg ccggggcggc cgctgtctgg ccgaagaggg
     1681 gagtccgggc caggaggagt ccggtcagca gcaggagcag ggcggcgccg cggcggtgct
     1741 ttagtttcat gttcgtctcc attatccagg ggtgcgcgag tgccggggcc gctttgacgg
     1801 cccggttctc aaggtaaaaa aataaacatt ttaaatgggc ggcccctttc tgtcaaacga
     1861 aacgggcacg cttgaaggtc ctttatttat cagttgagtt tcccgagatt tcgtttatcc
     1921 gggcgcgcgc ggccggcttt tccggtccgg ttttcccgct ttcaccccgg gtatcttgag
     1981 tcgcccggct tgacgccgcc cttcttttta agtaaaaagt attagtataa ttgattcgga
     2041 tggttttgtt ttgcacgaag aacatacttt atcgattcat ctcttttaca aaagcgcgag
     2101 gccttcatga agatcctgaa gcggtcaatc ataatggtcg cggccgcctg gggcctgctc
     2161 ggagcgtccc ttcttttcgg ggccacgccg gggagcctga agtggacctg cagttacggc
     2221 ggcaccaacc ggacctcgcc ggcggtggcc cgggacggca ccgtctatat cggcggcaac
     2281 tccgaattca aggcgatcaa cccctccgac ggcagcatcc gctggcgctt caccgtggcc
     2341 gggaacgagt tccacgcgcc gccggtcatc ctgccggac
//
LOCUS       DAOQYN010000252         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_50484_length_2367_cov_4.519464, whole genome shotgun sequence.
ACCESSION   DAOQYN010000252 DAOQYN010000000
VERSION     DAOQYN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50484_length_2367_cov_4.519464"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PKN80_08470"
     CDS             <1..1292
                     /locus_tag="PKN80_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36080.1"
                     /translation="ALYPYAATALTTIAANAGPTGLVESCFGGTDYSEQTNRPRFEDP
                     RDNRTLSSRLSGVEDIGLLFHACQQGAELAGALNDEKLLKTCLNLSRRIEAGFMGHFF
                     DAEEGFFIDCVWPKDKPVNRNRTFRLTSLLALSGHGELLSLDEWDRLADFIVRRLGHP
                     VIGLRECPADQPMHPTGRNRSENWFQNASRETFRLARLSGNQELLRILLEKAEFFFGA
                     ERVLRENLYNNQATQFFDASRLYVSTSWWQCMTTASWWLGLLEALAGIRLTQGNLEYL
                     PGDGGSDVRLTNLFYGGRVWEVDVRGRGRFVAGLEINGRPFDGTQQLEAADERPEQRV
                     RIEKTNRPPVHPVVLGAGAARARITRLEPGRLEAGLAAPGYCRLWFYSPEQPRLLVDG
                     RPIRFAWSPKRHEGTAGFRAGGDSRLEIRCPAAGRRP"
     gene            1443..2273
                     /locus_tag="PKN80_08475"
     CDS             1443..2273
                     /locus_tag="PKN80_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ36081.1"
                     /translation="MEKRHGFTLIELLVVIAIIAILAAMLLPALEKARNRAHKANCMN
                     NLKQCYLALMMYAYEYDEWFPYHGPWENGQPALFTPTTQFGVTKGWSTNSLRLLYPAY
                     IKNWNLMICPASEYKIPANLSSPDQITPLNIAYGYANGINLKWAQRWSLPIHDFALMS
                     DKYARINGDESTANLTVWNNNQDEIPAASHLVGTFYTQKGGGNHQDGANFLMLDGHVE
                     WRTAVKASDGVFYFGEATGSVWSESSRYFNLPVNQKAYSQIWQGFGKASTGHIALRCW
                     "
BASE COUNT          463 a          812 c          706 g          386 t
ORIGIN      
        1 gggcgctcta cccttacgcg gccacggccc tgacaaccat cgccgccaac gccgggccga
       61 ccgggctggt ggagtcctgc ttcggcggca ccgattacag cgagcagacc aaccggcccc
      121 gcttcgaaga cccgcgcgac aaccggaccc tttcctcgcg cctgagcggc gtcgaggata
      181 tcggcctgct cttccacgcc tgccagcagg gggccgaact ggccggcgcg ctcaatgatg
      241 aaaaactgct caagacctgc ctgaacctct cccgtcgcat cgaggccggt ttcatgggcc
      301 acttcttcga tgccgaggaa ggcttcttca tcgactgcgt ctggcccaag gataagccgg
      361 tcaaccggaa ccggaccttc cggctcacct cgctgctggc cctctccggc cacggcgaac
      421 tgctctccct tgacgagtgg gaccggctgg ccgatttcat cgtccgccgc ctcggccacc
      481 cggtcatcgg cctgcgcgaa tgcccggccg accagccgat gcacccgacc ggccgcaacc
      541 ggagcgaaaa ctggttccag aacgccagcc gggagacctt ccggctggcc cgcctctccg
      601 gcaaccagga actcctccgg atcctgctgg aaaaggccga gtttttcttc ggcgccgagc
      661 gggtgctgcg cgagaacctt tacaacaacc aggcgaccca gttcttcgac gcctccaggc
      721 tctacgtctc gacttcctgg tggcagtgca tgaccaccgc cagctggtgg ctgggcctgc
      781 tggaagcgct ggccggcatc cgcctcaccc agggaaacct cgagtacctg cctggcgacg
      841 gcggcagcga cgtccggctg accaacctgt tctacggagg ccgggtctgg gaagtggatg
      901 tccggggccg gggccgtttc gtggccgggc tggaaatcaa cggccgcccc ttcgacggca
      961 cccagcagct cgaagcggcc gacgaacggc cggaacagcg ggtgcggatc gagaagacca
     1021 accggccgcc ggtccacccg gttgtcctgg gcgccggcgc ggcccgggcc cgcatcaccc
     1081 ggctcgaacc gggtcgcctg gaggccggcc tggcggcgcc gggttactgc cgcctctggt
     1141 tctacagccc ggaacagccg cggctgctgg ttgacggccg gccgatccgt ttcgcctgga
     1201 gcccgaagcg gcacgagggt accgccggct tccgggccgg cggggattcc cggctggaga
     1261 tccgctgccc ggccgccgga cggcggcctt gacaaacggc ggaccggggg gtaaaatacc
     1321 agaatagaaa tcaatcgggt gtaatttttg gtttttttga ttttcagtta acgataacaa
     1381 aggaggcgcc ggcaagaaaa aatccctcgc cattcctaac ttttcacgat ggaggtctgg
     1441 aaatggaaaa gaggcacggt ttcaccctga tcgaactgct ggtggtcatt gccatcatcg
     1501 ccatcctggc ggccatgctg ctgccggccc tggagaaggc ccgcaaccgg gcccacaagg
     1561 ccaactgcat gaataacctc aagcaatgct acctcgccct gatgatgtac gcctacgagt
     1621 acgacgagtg gttcccctac cacggaccct gggagaacgg ccagccggcc ctctttacgc
     1681 ccaccaccca gttcggagtg accaagggct ggtccaccaa ctccctgcgc ctgctctacc
     1741 cggcttacat caagaactgg aacctgatga tctgcccggc ctccgaatac aagatcccgg
     1801 ccaacttgag ttcacccgac cagataacgc cgctgaacat cgcctatggc tacgccaacg
     1861 gcatcaacct gaagtgggcc cagcgctgga gcctgcccat ccacgacttc gccctgatga
     1921 gcgacaagta cgcccggatc aacggcgacg agtcgaccgc caacctgacc gtctggaaca
     1981 ataaccagga cgagataccg gccgccagcc acctggtcgg caccttctac acccagaagg
     2041 gcggcggcaa tcaccaggac ggggccaact tcctgatgct cgacggccac gtcgagtggc
     2101 ggaccgccgt caaggccagc gacggagttt tttatttcgg agaggcgacc ggctccgtct
     2161 ggtccgaatc cagccgttac ttcaacctgc cggtcaacca aaaggcctac agccagatct
     2221 ggcagggatt cggcaaggcc agcaccggcc acatcgccct gcgctgctgg taaaaaacgg
     2281 gagacggaga atgaaacggt cggataaaaa agagggcttc accctgatcg agctgctggt
     2341 ggtcatcagc atcatcgcca tcctggc
//
LOCUS       DAOQYN010000253         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_51836_length_2328_cov_3.223493, whole genome shotgun sequence.
ACCESSION   DAOQYN010000253 DAOQYN010000000
VERSION     DAOQYN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51836_length_2328_cov_3.223493"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKN80_08480"
     CDS             complement(<1..219)
                     /locus_tag="PKN80_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36082.1"
                     /translation="MAANETLFPEDKSGLDASELPLAVRMRPESLDEFVGQEEILGEG
                     KLLRRAILADRVTSLILFGPPGTGKTTLA"
     gene            complement(247..424)
                     /gene="ssrS"
                     /locus_tag="PKN80_08485"
     ncRNA           complement(247..424)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKN80_08485"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(502..2274)
                     /locus_tag="PKN80_08490"
     CDS             complement(502..2274)
                     /locus_tag="PKN80_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNQ36083.1"
                     /translation="MPKLLIDGKNVEVPEGTTILAAAEQAGIKIPTLCYLKDVQAIGA
                     CRVCVVEVEGYRNLVASCVTPVNEGMHVLTNSRRVRESRRTVLDLILSDHDGECQTCE
                     RNEDCELQMVSRALGMREITNTGEKSRKITDDRTPALDRNSAKCIHCRRCVTVCSEVQ
                     GVRALNPQGRGFKTIIGPAFNRGLDTVACVQCGQCAAVCPVGAISEKNHINAVWAALD
                     NPSLHVIVQTAPAIRATLGECFGYPPGTRVMGRMVAALRRLGFDGVFDTEFSADLTII
                     EEGNELLTRLKQALVEKKPVALPQFTSCCPGWIKYSEHFYPELLPNLSSCKSPQQMFG
                     ALAKSFYAEKLGKRPEDIFVVSVMPCTAKKFEAQRPEMNASGVQDVDAVLTTRELATM
                     IKQGGVDFDKLTDEAMDSPFGLTASGAADIFANTGGVMEAALRTVYEVVTGRELPLKG
                     LHVKPIAGLKGVKTASLVIKDALPEWSFLEGVTLKIGVAHGLENARRIIEKVKSGQEE
                     FHFIEVMTCPGGCIGGGGQPRLTSDKVRKLRIEAIYREDEGKKVRKSHESPEIKKIYK
                     DYLGKPLGKKSHHLLHTGYQKRES"
BASE COUNT          399 a          751 c          694 g          484 t
ORIGIN      
        1 ggccagggtc gtcttgccgg tgccgggcgg tccgaagaga atcaacgagg tgacccggtc
       61 ggccaggatg gcccgccgca gcagctttcc ctcgcccagg atctcctcct ggccgacgaa
      121 ttcatccagg ctttccgggc gcatccggac cgccagggga agctcggagg catccaggcc
      181 ggacttatct tccgggaaaa gagtttcgtt ggccgccatc tatcctcagc agggatatcc
      241 cgcaaaaaaa atcccgtcct gccgttcggg taatacctgt gaacccatca gtttaagtgg
      301 gtgtcatccc agggcggatt ttgcttctgc cttacggcag cctgcagcct tcgctctgaa
      361 tccgactccc aatgtacagg tgttggctca aggtatcaac ccgtggcgca caggacagga
      421 tcctactgcc tcgggattca agactatttt ataaccgcct gaccggcctg tcaagtcacg
      481 ccgccaaaca gccggcccgg gtcaactctc ccgtttctgg tagccggtgt gaagcaggtg
      541 atgggacttt ttgcccagcg gcttgcccag gtaatccttg tatattttct ttatttcggg
      601 gctctcgtgg gacttgcgca ccttctttcc ctcgtcctcc cggtaaatgg cctcgatcct
      661 cagtttccgg accttgtcgg aggttagacg cggctggccg ccgccgccga tgcagccccc
      721 cgggcaggtc atgacttcga tgaagtggaa ctcctcctgt ccggacttga ccttttcgat
      781 gatgcggcgg gcattctcca ggccgtgcgc cacgccgatc ttcagggtca cgccctccag
      841 gaaagaccac tccggcaggg cgtccttgat caccagcgag gccgtcttga cccccttgag
      901 gccggcgatc ggctttacgt gaaggccctt aagcggcagt tcccggccgg ttaccacctc
      961 gtaaacggtc cgcagggccg cctccataac cccgccggtg ttggcgaaga tgtcggccgc
     1021 gccgctggcg gtgaggccga agggggaatc catcgcctcg tccgtgagct tgtcgaaatc
     1081 gacgccgccc tgcttgatca tcgtcgccag ttctctcgtg gtgagaaccg cgtccacgtc
     1141 ctggacaccg ctggcgttca tctccggacg ctgggcctcg aatttcttgg cggtgcaggg
     1201 catgaccgaa accacgaaaa tatcttcggg ccgcttgccg agtttctcgg catagaatga
     1261 tttcgccagg gccccgaaca tctgctgggg cgacttgcaa ctggaaagat tcggcagcag
     1321 ttccgggtaa aagtgctcgc tgtacttgat ccagcccgga cagcaactgg tgaactgcgg
     1381 cagggcgacc ggcttcttct cgaccagagc ctgcttgagc cgggtcagca attcgttgcc
     1441 ttcctcgatg atcgtcaggt cggcgctgaa ctcggtgtcg aagaccccgt caaaaccgag
     1501 ccgacgcagg gcggcgacca tccggcccat cacccgggtc ccgggcggat acccgaaaca
     1561 ctcgcccagg gtggcccgga tggccggggc ggtctgtacg atcacgtgca gggacgggtt
     1621 gtccagggcc gcccagacgg cgttgatatg gttcttttcg ctgatggccc ccaccggaca
     1681 gacggccgcg cactggccgc actgaacgca ggcgaccgtg tccaggccgc ggttgaaggc
     1741 cgggccgatg atggtcttga aaccgcgccc ctgcgggttc agggctctta ccccctggac
     1801 ctcggagcag acggtcacgc accggcggca gtggatgcac ttggcactgt tccggtccag
     1861 cgccggggtc cggtcatccg taattttgcg gctcttctcc ccggtgttgg taatttcccg
     1921 cattcccagc gcgcggctga ccatttgcaa ttcgcaatcc tcgttccgct cgcaggtctg
     1981 gcattcgcca tcgtggtcgg aaaggatcag gtccaggacg gtccggcggc tttcgcgcac
     2041 ccgccgggaa ttggtcagca cgtgcatccc ttcgtttacc ggggtgacgc aggaagcgac
     2101 caggttccgg taaccctcga cctccaccac acagacccga caggccccga tggcctgaac
     2161 atccttcagg tagcagaggg tcgggatctt gatgccggcc tgctcggcgg ccgccaggat
     2221 ggtcgtccct tccggcacct cgacgttttt tccgtcaatc aagagtttgg gcatgtgatc
     2281 tccttggcca tcatttcttg cagcgataat cgcaccgcag gcagcgct
//
LOCUS       DAOQYN010000254         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_54034_length_2265_cov_3.666968, whole genome shotgun sequence.
ACCESSION   DAOQYN010000254 DAOQYN010000000
VERSION     DAOQYN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54034_length_2265_cov_3.666968"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PKN80_08495"
     CDS             <1..672
                     /locus_tag="PKN80_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNQ36084.1"
                     /translation="VRRQVSFGLKPAADYRVKRLKTGPDGTAFILERPDGLPLALTVP
                     LFGAFNLRNTAAACAAALEMGLPAEALAERLASFQPVPHRFAVRPWRRAFIVDDTYNA
                     NPTSFQAALAEFRRLAGPAPRIITAGSMGELGRFSRRSHLTLGRRLARLEPGLVILLG
                     PERATMAEGARQAGLPARRIRIAESNPAAAEILKKHLQPGAWALLKGSRLNRLEEILE
                     VLEKE"
     gene            850..2103
                     /locus_tag="PKN80_08500"
     CDS             850..2103
                     /locus_tag="PKN80_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36085.1"
                     /translation="MAGHRNDIKVLRELAKQYAEVAALPIQEERRQLWSEHNSLRPTR
                     VLVIATFGMWNVWCREIFNDGRMECRDPFYRYYERILRMQLFQHSIGDDFILEPWINH
                     RAAVRSSGGFVNPWGVEEKRINPGVEGGAFRFDPPLKEWSDLEKLRPLAHQVDEVETG
                     RGLNRLREAVGDILPVNLDRTPVWTGWLSDISTSLGRMRGLDQVMIDMYEAPEKLHGL
                     LAFMRDHILAQQAAAEAAGDFSLTSGHNQCHIYSRELEAPRANSGPRRRRQLWGYFAA
                     QEFTLVSPAMHEEFMLRYQRPIMENFGLTAYGCCEDLTRKIGILRQVSNLRVIAVAPR
                     ADLAACARAAGTDYVLSWRPNPADMLCCGFDPDRVRRIITAGLESARGCRVHIHLKDI
                     ETLEGDPDRLRRWVQVVRSACDRVH"
BASE COUNT          382 a          835 c          707 g          341 t
ORIGIN      
        1 gtgcgccgcc aggtcagctt cggcctcaag ccggccgccg actaccgggt gaaacgcctc
       61 aagaccggcc ccgacggcac cgccttcatc ctggagcggc ccgacggact gcccctggcg
      121 ctgaccgttc ccctcttcgg cgccttcaac ctgcgcaaca ccgcggccgc ctgcgccgcc
      181 gccctcgaga tgggcctgcc ggccgaagcc ctggccgaac ggctggcctc cttccagccg
      241 gtgccgcacc gcttcgcggt ccggccctgg cgacgcgcct tcatcgtcga cgacacctac
      301 aacgccaacc ccacctcctt ccaggccgcc ctggccgaat tccggcgcct ggccggtccg
      361 gcgccccgga ttatcaccgc cggctcgatg ggcgaactgg gccgcttcag ccggaggagc
      421 cacctgaccc tgggccgccg gctggcccgg ctggaaccgg ggctggtcat cctgctgggg
      481 ccggaacggg ccacgatggc cgaaggcgcc cgacaggccg gcctgccggc ccgccgcatc
      541 cgaatcgccg aaagcaaccc ggccgccgcc gaaatcctga agaagcacct ccagcccggc
      601 gcctgggccc tgctcaaggg ctcccgtctc aaccgcctcg aagaaatcct ggaagtgctg
      661 gagaaagaat agccgttcgc cccggattcc agaggcggca attaaaaccc gatatgaaag
      721 ggatggtaac tgatttgcgc tgtttagctg ttacgctgtt acaccattat tatctgccgg
      781 acgccgtcat tgatcggacc gccggcgccg gaacctctct aattccatca ttaccattgg
      841 aggcataagg tggccggaca tcggaacgac atcaaggtac tgcgcgaact ggcgaagcag
      901 tatgccgagg tggcggcgct ccccatccag gaggagcggc ggcagctctg gagcgaacac
      961 aacagcctgc gtcccacccg ggtgctggtc atcgccacct tcggcatgtg gaacgtctgg
     1021 tgccgagaga tcttcaacga cggccgaatg gagtgccgcg acccgttcta ccgctactac
     1081 gagcgcatcc tgcgcatgca gctcttccag cactcgatcg gcgacgattt catcctcgag
     1141 ccctggatca accaccgggc cgcggtccgg agcagcggcg gcttcgtcaa tccctgggga
     1201 gtcgaggaga agcggatcaa cccgggcgtg gaaggcggcg ccttccggtt cgacccgccc
     1261 ctcaaggagt ggtccgacct ggagaaactg cggccgctgg cccaccaggt ggacgaggtg
     1321 gagaccggcc gcggcctcaa ccgcctccgg gaggcggtcg gcgacatcct gccggtcaac
     1381 ctcgaccgca ccccggtctg gaccggctgg ctctcggaca tctcgacctc gctgggccgc
     1441 atgcgcggac tcgaccaggt gatgatcgac atgtacgagg cgccggaaaa actgcacgga
     1501 ctgctggcct tcatgcgcga ccatatcctg gcccagcagg cggcggccga ggcggccggc
     1561 gacttctccc tgaccagcgg ccataaccag tgccacatct acagccggga actggaggcg
     1621 ccccgggcca actccgggcc gcgccggcgc cgccagctct ggggctactt tgccgcccag
     1681 gagttcacgc tggtctcgcc ggcgatgcac gaggagttca tgctccgcta ccagcggccg
     1741 atcatggaga acttcggcct gaccgcctac ggctgctgcg aggacctgac ccgcaagatc
     1801 ggcatcctgc gccaggtctc caacctgcgc gtcatcgcgg tggccccgcg ggccgacctg
     1861 gccgcctgcg cccgggcggc cggcaccgat tacgtgctct cctggcgacc caacccggcc
     1921 gacatgctct gctgcggctt cgacccggac cgggtgcgcc ggatcatcac cgccggactg
     1981 gagtcggccc gcggctgccg ggtacacatc catctcaagg atatcgagac gctcgagggc
     2041 gacccggacc gcctgcggcg ctgggtgcag gtggtccgct cggcctgcga ccgcgtccat
     2101 tgaaagaacc ggcccggccc ccggttgtcc gccggggcgg cttctgctac aatatggcca
     2161 tggaccgaat ccccgaaatc agcaagcggc tggcccgggc ctacccgaac acccggaccg
     2221 ccctcaagtt ccagacgccc tttcaactgc tggtcgccac catcc
//
LOCUS       DAOQYN010000255         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_54562_length_2252_cov_3.134729, whole genome shotgun sequence.
ACCESSION   DAOQYN010000255 DAOQYN010000000
VERSION     DAOQYN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54562_length_2252_cov_3.134729"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..971
                     /gene="rsmH"
                     /locus_tag="PKN80_08505"
     CDS             84..971
                     /gene="rsmH"
                     /locus_tag="PKN80_08505"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNQ36086.1"
                     /translation="MRHVPVLTREVLEILQPGRGGLFIDATAGNGGHSRAILENSPAD
                     LRLIAIDRNPATLGVAAEHLEPFQNRVRLLQGDFRDLAALLPGQTPGTVEAILYDLGL
                     SSFLLDQPDLGLSFQRDDPLDMRFDPGAGFPAYQLLAETPEPELADLFYQLGEIRESR
                     RLAARIKSARPPVQTTGQLAAIARSVIRRRGRIDPATRVFQALRIAVNDELDALRETL
                     PQAEKLLAPGGRLAVISYHSLEDRIVKNFLRESRELERLTRKPIQPSEAERRENPASR
                     SAKLRGARKLPEAVFLTGK"
     gene            1016..1390
                     /locus_tag="PKN80_08510"
     CDS             1016..1390
                     /locus_tag="PKN80_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36087.1"
                     /translation="MRRKKRRSLGFWQYFGLAFLGVLLYVWQYGLSFNIAAQIPALES
                     RSKDLQAEHRQLEVQALALKNAAAIRERVARFNLPLVEPNAWNVYRIEPADRSRAETR
                     TDTDQDRPAKSTAPPVRIRPTL"
     gene            1387..>2252
                     /locus_tag="PKN80_08515"
     CDS             1387..>2252
                     /locus_tag="PKN80_08515"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36088.1"
                     /translation="MKLPGFRKREARLQLVVWVYLIFALVIIFKLGILQIKHGARYRL
                     EAQKSHFLKDEIPAPRGYIYDRNGQPLAISIPTYSLYAEPRYLALTDTPLEVARKLAP
                     VLEMPVDQLNAILSSGKGFAWIQRRLPYETYAKIRDLGLSGKIGFEEHYERSYPEGRL
                     ACHIIGTTNLDGQGIEGLEKEYDRYLTGRKGNYEVFRDGRGRRLPWEYSSQPAIPGRN
                     LHLTIDLRLQRILEEEIDVIQQKHTPRGIMAIILDADTGEVLALTNRPNYDPNRPAPA
                     LEPRFHLPGSRV"
BASE COUNT          420 a          855 c          637 g          340 t
ORIGIN      
        1 atggctgcct aaacaggacc cggcttatca cccccgccgg cagtacccgt cccggtgccg
       61 gtttccacca acctggtatc cccgtgcggc acgtaccggt cctcacccgc gaagtcctgg
      121 aaatcctgca gcccggccgc ggcggcctct tcattgacgc caccgccggc aacggcggcc
      181 acagccgggc catcctggaa aacagcccgg ccgacctgcg gctgatcgcc attgaccgca
      241 acccggccac cctcggcgtc gcggccgaac acctggaacc ctttcaaaac cgggtgcgcc
      301 tgctccaggg cgatttccgc gacctggccg cgctcctgcc cggccagacg ccgggaacgg
      361 tcgaggccat cctttacgac ctgggcctct ccagcttcct gctcgatcaa ccggacctgg
      421 gcctctcctt ccagcgcgac gacccgctcg acatgcgctt cgaccccggc gccggcttcc
      481 ccgcttacca gctcctggcc gaaaccccgg aaccggagct ggccgacctt ttttaccagc
      541 tgggcgaaat ccgggagagc cggcgcctgg ccgcccggat caagtcggcc cggccgccgg
      601 tccaaaccac cggccagctg gccgccatcg cccgctcggt gatccgccgg cgcggccgga
      661 ttgacccggc cacccgggtc ttccaggccc tgcgcatcgc cgtcaacgat gaactggacg
      721 ccctccggga gacgctcccc caggccgaaa aactgctggc cccgggcggc cggctggcag
      781 tcatctccta ccactcgctc gaggaccgaa tcgtcaagaa cttcctccgg gaaagccggg
      841 agctggaacg gctgacccgg aagcccatcc agccctccga ggccgaacgc cgtgaaaacc
      901 cggccagccg gagcgccaag ctgcgggggg cccgcaaact gccggaggcc gtttttttga
      961 ctggaaaata aatagccctt ataatttact ttaaaagata tggggtgccc aaacgatgcg
     1021 caggaaaaag agacgctccc tcggattctg gcagtacttc ggcctggcct tcctgggcgt
     1081 gctcctctac gtgtggcagt atggcctctc cttcaacatc gccgcacaga tcccggccct
     1141 ggagagccgg tccaaggacc tccaggccga acaccgccag ctcgaggtcc aggcgctcgc
     1201 cctgaagaac gccgccgcca tccgggaacg ggtggcccgt tttaacctgc ccctggtcga
     1261 acccaacgcc tggaacgtct accgcatcga acccgcggat cgaagccgcg ccgagacgcg
     1321 gaccgacacc gaccaggacc ggcccgccaa aagcacggcg ccgccggtcc gtatccggcc
     1381 caccctatga aactgcccgg tttcaggaaa cgcgaagccc gccttcagct ggtcgtctgg
     1441 gtttacctga tcttcgccct ggtaataatc ttcaagctcg gtatcctgca gatcaaacac
     1501 ggcgcgcgct accgcctcga ggcccagaag agccatttcc tcaaggatga gatcccggct
     1561 ccccgcggat acatctacga ccgcaacggc cagccgctgg ccatctcgat tcccacttac
     1621 tccctctatg ccgaaccccg ttacctggcc ctgaccgaca ccccgctgga ggtcgcccga
     1681 aaactggccc ccgtgctgga gatgccggtc gaccagctga acgccatcct ctcctccggc
     1741 aagggcttcg cctggatcca gcgccgactc ccctacgaga cctacgccaa gatccgcgac
     1801 ctgggcctct ccggaaagat cggcttcgag gagcattacg aacgctccta cccggaagga
     1861 cgcctggcct gccacataat cggcaccacc aatctggacg gccagggcat cgagggcctc
     1921 gagaaggagt atgaccgcta cctgaccggc cggaagggca attacgaggt cttccgggac
     1981 ggccgcggcc ggcggttgcc ctgggaatac agcagccagc ccgccatccc cggccggaac
     2041 ctgcacctca ccatcgacct gcgcctccag cgtatcctcg aggaagagat cgacgtcatc
     2101 cagcagaagc atacgccccg gggcatcatg gccatcatcc tggacgccga caccggggag
     2161 gtcctggccc tgaccaaccg gcccaactac gatccgaacc ggccggcgcc ggccctggag
     2221 ccgcggttcc acctgccggg ctcgcgcgtg at
//
LOCUS       DAOQYN010000256         2221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_55751_length_2221_cov_3.722992, whole genome shotgun sequence.
ACCESSION   DAOQYN010000256 DAOQYN010000000
VERSION     DAOQYN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55751_length_2221_cov_3.722992"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PKN80_08520"
     CDS             complement(<1..790)
                     /locus_tag="PKN80_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ36089.1"
                     /translation="MAGFFLATWLAILAVPPSAESYTEREWIQLARVAYENGFHDLSL
                     EYLDQFQKQYPESGQAAYGLILRGLNLRKQEKTAEAGRIFSDLIDAASPHSYRSLAYY
                     LRGETNFARSRYPEAAEDFRNALAGTLEAENENPARQGLVLSLAYQGRYAEAIQALTE
                     WRGRPNGSGREWRETRSNLIKLASGDLFQAIKQKDYQRVQSMAALLTERLDRDSQLDL
                     VRYYQASAWLESGQRETALVQLEALTRSAGDDLAALAAFRLGDLF"
     gene            complement(906..1112)
                     /locus_tag="PKN80_08525"
     CDS             complement(906..1112)
                     /locus_tag="PKN80_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010630860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNQ36090.1"
                     /translation="MARIKGKVKWFNAAKGYGFIEREGGQDVFVHYSAISGEGYKSLD
                     QGAEVEFDLVESDKGPQAENVAKL"
     gene            complement(1360..>2221)
                     /locus_tag="PKN80_08530"
     CDS             complement(1360..>2221)
                     /locus_tag="PKN80_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNQ36091.1"
                     /translation="VDLRLLKKRRLKVVVDAVNGAAGYPAARLLERLGAEFFLLNGEP
                     TGLFTHPPEPRPEHLGQLAEEVRRREADLGLALDPDGDRLALISESGIPLSEELTLAF
                     CVEAVLEKKPGGVVTNLSTSQMVEEIAARFAVPFERTPVGEFFVARRMLELDAVIGGE
                     GNGGVILPACHPGRDGLVAAALLLELLARRNEPVSRLAARLDRFCLLKERVEIVGGRP
                     PEPDRLKLDALFNGIQPDTRDGLRYSGPGWWCHIRVSNTEPIVRILAEAADREAAARI
                     VEGVRGLLRS"
BASE COUNT          430 a          732 c          623 g          436 t
ORIGIN      
        1 ggaaaagatc acccagcctg aaggccgcca gcgcggccag gtcgtcaccg gccgaccgag
       61 tcaaagcctc cagttgaacc agggccgtct cccgctgccc ggattccagc caggcactgg
      121 cctggtagta acgaaccagg tccagctgag aatccctgtc cagccgttcg gtgagcaggg
      181 cggccatcga ctgaacccgt tggtaatcct tctgcttgat ggcctggaac agatcgccgg
      241 aggccagctt gatcaggttg gacctggtct cgcgccactc ccttcccgac ccgttcggcc
      301 gtcctctcca ttccgtaagc gcctggatcg cctcggcata ccggccctgg taagccaggg
      361 aaaggaccaa tccctgccgg gccgggttct cgttttcggc ctcgagggtc ccggccaggg
      421 cgtttcggaa atcttcggcc gcctccgggt agcgggaccg ggcaaagttg gtttcgccgc
      481 gcaggtagta agccagcgaa cgataagagt ggggggaggc ggcatcgatc aggtcggaga
      541 aaatgcggcc ggcctccgcc gttttctcct gtttccggag gttgagaccg cgcaggataa
      601 gaccgtaagc agcctgaccg ctctcgggat attgcttctg aaattgatcc aggtattcca
      661 gggaaagatc atgaaaaccg ttttcgtaag ccacccgggc cagctggatc cattcccgct
      721 ccgtgtagga ctcggcgctg ggcggtacgg ccagaatggc cagccaggtt gccagaaaaa
      781 agccggccaa cagacgaaaa cctgttctca tttgcatccc ggcggatccg tccgccaaaa
      841 gaaagccccg gctttcgccg gggctttcgt cgttccatta cagagatagt tcttacgatc
      901 tatccttata acttcgcgac attttccgct tggggtcctt tgtcgctctc aacgagatcg
      961 aattccactt cggcgccctg gtcgagcgac ttgtatcctt cgccgctgat agcggagtag
     1021 tggacgaaca catcctgccc accttcccgt tcaataaagc cgtaaccctt ggctgcattg
     1081 aaccacttca ctttaccctt aatacgtgcc attcttactt cccctccttt ccttctcccg
     1141 gctcactcca actaaacgga tgtccgcgag tcaatatgaa ctattacttg ctgctggtag
     1201 ccccgccgcg gggctcgagt tccatcttca tggaacaccc ctggccggcc accgccagcg
     1261 ccagaagtat ggccatcaaa acaaacagtc ttttcctcat tttcggtcct ccgtaaatta
     1321 ttactttccg aaaactttac cacaagggaa gccgggctgt caagaccgca ggaggccccg
     1381 gacgccctcc acgatccggg ccgccgcttc ccggtcggcc gcctcggcca ggatccgcac
     1441 gatcggttcg gtgttggaaa cgcggatgtg gcaccaccag cccggaccgc tgtaacgaag
     1501 cccatcccgg gtgtcgggct ggataccgtt gaacagcgcg tccaatttta accggtctgg
     1561 ttccggtggg cggccgccga caatctcgac ccgttccttg agcaggcaaa aacggtccag
     1621 ccgggcggcc agtctggaaa ccggttcatt ccgacgggcc agcagttcga gcaggagcgc
     1681 cgccgccacc aggccgtccc ggccgggatg gcaggccggc aggatcaccc cgccgttgcc
     1741 ttccccgccg atcacggcgt caagctccag catcctcctg gccacaaaaa actcgcccac
     1801 cggggtccgt tcaaagggaa ccgcgaaacg ggcggcgatt tcttcgacca tctggctggt
     1861 cgaaaggttg gtcactacgc ccccgggctt cttttcgagt accgcctcga cacagaaggc
     1921 cagggtcaac tcctctgaga gaggaatccc ggattcggag atcagggcca gacgatcccc
     1981 gtccgggtcc agggccagcc cgagatcggc ctcccggcgg cgcacctctt ccgccagctg
     2041 cccgaggtgt tccggcctgg gttcgggagg gtgtgtgaac aatccggtcg gctccccgtt
     2101 cagtagaaaa aattcggctc ccagcctctc cagcaggcgg gcggccggat aaccggccgc
     2161 accgttgacg gcgtccacaa ccaccttcaa tcttctcttt ttcagaaggc ggaggtccac
     2221 c
//
LOCUS       DAOQYN010000257         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_56166_length_2210_cov_4.486311, whole genome shotgun sequence.
ACCESSION   DAOQYN010000257 DAOQYN010000000
VERSION     DAOQYN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56166_length_2210_cov_4.486311"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PKN80_08535"
     CDS             <1..692
                     /locus_tag="PKN80_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNQ36092.1"
                     /translation="VLLKRLYDRGCEIGNHTFTHNNDGFDYLRLPEHEMEAEFRQCQS
                     WLRDRIPGLERVYAFWSGGSGFRPREKTKMAGERSRELAVEWGLATDIPYDWPRSFNP
                     DFLAPPYHDPGKFVSWHTRNDLSSAYMFDSAEEGIEALKSSFRVFYANPGGRPMFFGH
                     HDWPLGEEEIRPGVKLTYEVLWGFLKWALVEERNRFPNFKGITPLEYAYITAGRAAEL
                     ENRKEPLQNGR"
     gene            749..1978
                     /locus_tag="PKN80_08540"
     CDS             749..1978
                     /locus_tag="PKN80_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36093.1"
                     /translation="MEKGFSDKNLSSGRRFKLTEQIREVETGARAHYFIHEEPSQEQI
                     FYYCSPAISDDGRYIPCCSNVSGAWQIHVIDRREKVSIQLSNIEGGVPVGDPHCYLPE
                     KNQIFFIDRRSAYAANIEDASVLRLFEAPEDHRITSLSARGNYLAFSYCERVARGRLP
                     EGRHFSGYPALSHRPRSIIVAIDLRTGDAAEVWGDYSCLCHVELSPVDDDLVMFCDQS
                     WERRQQEVYLVRRSFCEDKRAWPVLAAPGGDYRGRTLDYIGHSFFTRDGQVAAQYCEY
                     GNLDAKMNFTDTAMFNLVIDPDGYHKRKAKFPGSGKPTHVHCQTAGGLWVGDGWARPD
                     GRLDNGWISLIRNNFETQETENWPLLPTNHTWDRPFHPHPWISPNEDFVVLAHNTGKS
                     DNHMVLVELPERFRTGE"
     gene            1996..>2210
                     /locus_tag="PKN80_08545"
     CDS             1996..>2210
                     /locus_tag="PKN80_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36094.1"
                     /translation="MAPGKNRVRDLYRGLRRRLGAPAGQWESWCRRPKSRGERERVIL
                     ESILTQRANWKNVQQAVENLSAAGSLGL"
BASE COUNT          435 a          741 c          687 g          347 t
ORIGIN      
        1 ccgtcctgct gaaacgcctc tacgaccggg gctgtgaaat cggcaaccac accttcaccc
       61 acaacaacga cggcttcgac tacctgcggc tgccggagca cgagatggag gccgagttca
      121 ggcagtgcca gagctggctc cgcgaccgaa tcccgggtct ggagcgggtc tacgccttct
      181 ggtccggcgg cagcggcttc cggccgcgcg aaaagacgaa gatggcgggg gaacggagcc
      241 gcgaactggc cgtcgaatgg ggcctggcca ccgacattcc ctatgactgg ccccgcagtt
      301 tcaaccccga cttcctcgcc ccgccttacc acgacccggg caagttcgtc tcctggcaca
      361 cgcggaacga cctctccagc gcctacatgt tcgactcggc cgaggaaggc atcgaggccc
      421 tcaagagcag cttccgcgtc ttctacgcga accccggcgg ccgccccatg ttcttcggcc
      481 accatgactg gccgctcggg gaggaggaga tccggcccgg tgtaaagctg acctacgaag
      541 tcctctgggg cttcttgaaa tgggcgctgg tcgaggaacg gaaccgattc cccaatttca
      601 agggaatcac gccgctggag tacgcctata tcacggccgg gcgggcggcg gagctcgaaa
      661 acagaaagga accgcttcag aacggccggt aattaattcc agggaagggc tgaagaatcg
      721 cccgttacgc caaaacatcg gagccgcaat ggaaaaggga ttttcggata agaacctgtc
      781 gagcggacgg cgcttcaagc tgaccgaaca gatccgggag gtggagacgg gcgcccgggc
      841 ccattacttc atccacgagg agccgtccca ggagcagatc ttctactact gcagcccggc
      901 catctcggac gacggccgct acatcccctg ctgcagcaat gtctcgggcg cctggcagat
      961 ccatgtcatc gaccgccgcg agaaggtctc catccagctg tcaaatattg agggcggcgt
     1021 tccggtcggc gacccccact gctacctgcc cgaaaaaaac cagatcttct tcatcgaccg
     1081 ccggagcgcc tatgccgcca atatcgaaga cgcctcggtg ctccgcctct ttgaggcgcc
     1141 cgaagaccat cggataacgt ctctctccgc gcgcggcaat tacctggcct tctcttactg
     1201 cgagcgtgtc gcccgggggc ggctgcccga gggcaggcat ttttcgggat atccggccct
     1261 ttcccaccgg ccgcgttcga tcatcgtggc aatcgacctg cgcaccggcg acgccgccga
     1321 ggtctggggc gattacagtt gcctttgcca cgtcgagctc tccccggtcg acgacgacct
     1381 ggtcatgttc tgcgaccaga gctgggaacg gcgccagcag gaggtatacc tggtgcgccg
     1441 gagcttctgc gaggacaagc gggcctggcc ggtgctggcc gcgcccggcg gagattaccg
     1501 gggccggaca ctcgactaca tcggccacag cttcttcacc cgggacggtc aagttgccgc
     1561 gcagtactgc gagtacggca acctcgacgc gaagatgaac ttcaccgaca cggccatgtt
     1621 caacctggtc atcgaccccg acggctacca caagcgcaag gcgaagttcc cgggcagcgg
     1681 caagcccacc cacgtccact gccagaccgc cggcggcctc tgggtcggcg acggctgggc
     1741 caggccggac ggacgcctcg acaatggctg gatcagcctg atccggaaca acttcgagac
     1801 gcaggagacc gagaactggc ccctgcttcc gaccaaccat acctgggacc ggcccttcca
     1861 cccgcacccg tggatctcgc ccaacgagga tttcgtggtc ctggcccata acaccggaaa
     1921 gtccgacaac cacatggtcc tggtcgagct gccggaacgt ttccggaccg gggaataaga
     1981 acggcgggag accggatggc gccagggaaa aaccgggtcc gggacctcta ccgcggcctg
     2041 cgccggcggc tgggcgcgcc ggccggccaa tgggagtcct ggtgccggcg gcccaagagc
     2101 cgcggggagc gggaacgggt catcctggaa tccatcctga cccagcgggc caactggaag
     2161 aatgtccagc aggcggttga aaacctgtcg gccgccggca gcctggggct
//
LOCUS       DAOQYN010000258         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_56915_length_2193_cov_3.050514, whole genome shotgun sequence.
ACCESSION   DAOQYN010000258 DAOQYN010000000
VERSION     DAOQYN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56915_length_2193_cov_3.050514"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKN80_08550"
     CDS             complement(<1..200)
                     /locus_tag="PKN80_08550"
                     /inference="COORDINATES: protein motif:HMM:NF019814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNQ36095.1"
                     /translation="MNANDLRPGMVFLQNGELHEVITFQHVKPGKGPAYIKCKLRAVK
                     TGKTFEISLRSSEKVEVIKMSE"
     gene            complement(235..1245)
                     /gene="pheS"
                     /locus_tag="PKN80_08555"
     CDS             complement(235..1245)
                     /gene="pheS"
                     /locus_tag="PKN80_08555"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNQ36096.1"
                     /translation="MPKEMIAKLEAELAEGLAAARTEAALEELRIRFLGRKSGRLNDI
                     LRGLKELPPEERREVGAAANRLKAEIENRLEEARLRLNAAAAPEVDLTLPGPPVRVGH
                     RHPLTLVGRELVSIFEKMGFDYREGPEVETDYYNFDALNTPSNHPARDLHDTFYLAPG
                     VLLRTHTSSVQIRVMEREKPPVRIVTLGRCYRRDAQDASHSPTFHQFEGLMVEKGTSF
                     IHLRGVLNLFLEKVFPFPVKTRFTPAFFPFTEPSAEVGISCPVCQARGCPSCGHNGFL
                     EVLGCGMVHPRVLKNVGYDPDKVTGFAFGMGVERIAMIKYGIKDIRLFLENDVRFLHQ
                     FS"
     gene            complement(1276..>2193)
                     /locus_tag="PKN80_08560"
     CDS             complement(1276..>2193)
                     /locus_tag="PKN80_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ36097.1"
                     /translation="VCDPDPERRRQAEERFGAAVYAEHREMLGQAAPELTVIVSRSDQ
                     HCRHACDALAAGSHVLVTKPMCLDEAQARQMLEAERASGRRLFPFLPARWGADLRRLQ
                     ALVAAGAVGRVFCIRRSARGFGIRNDWQTERRYGGGYLLNWGPHLVDPPVLLAGGRPV
                     SVFGRTRQVMNPGDVEDVFYAVLTLDNGVLVQAEYTMMAADFPSWVIQGDAGTILVRG
                     DELVLRRTSPGSGPAAAGGYLKRLELTETQEKISGQLYGDAAEIYRDLAAALLEGKSF
                     PVSSADGLELARWLEAIKISAAGDRVVQL"
BASE COUNT          354 a          762 c          709 g          368 t
ORIGIN      
        1 tcctcgctca tcttgatcac ctccaccttc tccgaggagc gcagggagat ctcgaaggtc
       61 ttgccggtct tgacggcgcg cagcttgcac ttgatatagg ccggcccctt ccccggcttc
      121 acgtgctgaa aggtgatcac ctcgtggagt tcaccgttct gcagaaatac catgcccgga
      181 cgcaggtcgt tcgcattcat cttctctggc cgctcccatg ttgttggcgt aaattcagct
      241 gaactggtgc aggaaccgga catcattctc caggaagagc ctgatatcct tgatcccgta
      301 cttgatcatc gcaatccgct ccacgcccat cccgaaggcg aacccggtca ccttgtccgg
      361 gtcatagccg acgttcttga ggacccgcgg gtggaccatg ccgcagccca gcacctccag
      421 gaacccgttg tggccgcagg aagggcagcc ccgggcctgg cagaccgggc aggagatacc
      481 cacctcggcg ctcggctcgg tgaaggggaa gaaggccggc gtgaagcgcg tcttgaccgg
      541 aaagggaaag accttctcca ggaatagatt tagcacgccc cggaggtgga tgaaactggt
      601 ccccttttcg accatcagcc cctcgaactg gtggaaggtg ggcgaatggc tggcatcctg
      661 ggcatcccgc cggtaacagc ggcccagggt gactatccgc accggcggct tctcgcgctc
      721 catcacccgg atctggaccg acgaggtgtg ggtgcgcagc aggaccccgg gcgccaggta
      781 gaaggtatcg tgaaggtcgc gggccgggtg gttggatgga gtgttcaggg cgtcgaaatt
      841 gtaatagtcg gtctcgacct ccggaccctc gcggtagtcg aagcccatct tctcgaagat
      901 ggaaaccagc tcccgcccga ccagggtcag gggatgccgg tggccgaccc ggaccggcgg
      961 ccccggcagg gtcaggtcaa cctcgggcgc cgcggcggcg ttgaggcgga gccgcgcctc
     1021 ctccagccgg ttctcgatct ccgccttgag ccggttggcg gccgcgccca cctcgcgccg
     1081 ctcctccggc ggcagctcct tgagaccgcg cagaatgtcg ttgagccggc cgctcttgcg
     1141 gcccaggaaa cggatccgga gctcctccag cgccgcctcg gtccgggccg ccgccagccc
     1201 ctcggccagt tcggcctcca gcttggcgat catctcttta ggcatgcttg ggttcctcct
     1261 ctccgcgtcg gccggtcaga gctggaccac ccggtccccg gccgccgaaa ttttaatcgc
     1321 ttccagccag cgggccagct ccagtccgtc ggccgagctg accgggaagg actttccctc
     1381 gagcagcgcg gccgccaggt cgcggtaaat ctcggccgcg tccccgtaaa gctgtccgct
     1441 gatcttctct tgcgtttcgg tcaattccag ccgtttcagg taaccgccgg ccgcggccgg
     1501 acccgatccc ggactggtcc ggcgcagcac cagttcgtcg ccccggacca ggatggtgcc
     1561 ggcgtcgccc tggatcaccc aggagggaaa atcagccgcc atcatggtgt actcggcctg
     1621 aaccagcacc ccgttgtcga gggtgaggac ggcgtaaaaa acgtcctcga catcgcccgg
     1681 gttcatcacc tgccgggtgc ggccgaagac cgagaccggc cggccgccgg ccagcagcac
     1741 cggcggatcg accaggtgcg gcccccagtt gagcaggtac ccgccgccgt agcgccgctc
     1801 ggtctgccag tcgttccgga tcccgaagcc tctggcgctc cggcggatgc agaagacccg
     1861 gccgaccgcc ccggcggcca ccagcgcctg gaggcgccgg aggtcggccc cccaacgggc
     1921 gggcagaaag ggaaagagcc gccggccgga ggcccgttcg gcctccagca tctgccgggc
     1981 ctgggcttcg tccagacaca tcggcttggt gaccagcacg tgggagccgg ccgccagcgc
     2041 gtcgcaggcg tggcggcaat gctggtcgct ccggctgacg atcaccgtca gttccggggc
     2101 ggcctggcca agcatctccc ggtgctcggc gtaaacggcg gccccaaagc gttcttcggc
     2161 ctggcgccgc cgttccgggt ccgggtcgca gac
//
LOCUS       DAOQYN010000259         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_57223_length_2185_cov_3.329108, whole genome shotgun sequence.
ACCESSION   DAOQYN010000259 DAOQYN010000000
VERSION     DAOQYN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57223_length_2185_cov_3.329108"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PKN80_08565"
     CDS             <1..1183
                     /locus_tag="PKN80_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36098.1"
                     /translation="ARTVPDVLGIRFQSRVVQSLAALVMIFFTTLYAVGQFKAGATCW
                     HAVTGLPTFWCLLLSTVVVFIYMIVGGYTGTQWSLAIQGAVLGVACFALGLAALVFVG
                     GPAAMNLKLMAENPSLLALMRTDLPATGNTQLFSHPVGIVSTFFIFLTMAVGFPHNVA
                     RFLGMRKMTRKDMMLLTLMVFLVAGPPIFLNAITGLCSRAHFGPQLLGLAPWKADLAA
                     PYLAMAAGGTFLSTLYITGVFAAALSTLAGMLMVMAGNVTRDIIHIWRPQTPDKVLLK
                     LTKVLLAIFLLIPFYWTFHKPPELLAVFMGYASIGLGGIFIFCTAASFYWRRATASGA
                     ILCMVYGVLSTLIGAIFTARGKIGMGTLEFIVLIGCGLCYFLGSYLQKPLPAEKLKEL
                     F"
     gene            1204..1758
                     /locus_tag="PKN80_08570"
     CDS             1204..1758
                     /locus_tag="PKN80_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ36099.1"
                     /translation="MKENLKRIAAEVAASLEKIEPGQFEAAAAEILKAPNLFLAGAGR
                     SGRMVEAFAIRLAQLGLSAYLAGGATTPAITAGDLLVIGSGSGRTAGLLAAAQTAREA
                     GARLLIFTAEPESPLARLADRLVVIPAGGRRRAGQGRPNSIQPLATLFEQSLLLLLDG
                     LVLRLMKARRVSAAAMAGRHANLE"
     gene            complement(1848..>2185)
                     /locus_tag="PKN80_08575"
     CDS             complement(1848..>2185)
                     /locus_tag="PKN80_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36100.1"
                     /translation="AEPIYSPWEQPPGNPRYREDYFHYFPIYRKMTRPGTHVFTEVQD
                     QDLLAGRFWRDLVLSAFVNEEFYLAAASYFHREETLVFKEVWEDAETGRRSREWKISP
                     WEIKIFRRP"
BASE COUNT          325 a          781 c          690 g          389 t
ORIGIN      
        1 ggcccggacg gtgccggacg ttctcggcat aaggttccag agccgggtcg tccagtcgct
       61 ggcggccctg gtcatgatct tcttcaccac cctctacgcg gtcggccagt tcaaggccgg
      121 cgccacctgc tggcacgcgg taaccggcct gcccaccttc tggtgcctgc tgctctcgac
      181 ggtggtggtc ttcatctaca tgatcgtggg cggctacacc ggcacccagt ggtcgctggc
      241 catccagggg gcggtcctgg gcgtggcctg cttcgcgctg ggcctggccg ccctggtctt
      301 cgtcggcggc ccggcggcca tgaacctgaa gctgatggcc gagaatccca gcctgctggc
      361 gttgatgcgg accgacctgc cggccaccgg caacacccag ctcttctcgc acccggtcgg
      421 catcgtctcc accttcttca tcttcctgac catggccgtc ggcttcccgc acaacgtcgc
      481 ccgtttcctg ggcatgcgga agatgacccg gaaggacatg atgctgctga cgctgatggt
      541 cttcctggtg gccgggccgc ccatcttcct gaatgccatc accggcctct gcagccgggc
      601 ccacttcgga ccccagctgc tgggcctcgc gccctggaag gccgacctgg ccgcgcccta
      661 cctggcgatg gcggccggcg gcaccttcct ctcgacgctc tacatcaccg gcgtcttcgc
      721 ggccgccctc tcgaccctgg ccgggatgct gatggtgatg gccggcaacg tgacccggga
      781 catcatccac atctggcggc cgcagacccc ggacaaagtc ctgctgaaac tcaccaaggt
      841 cctgctggcc atcttcctgc tcattccctt ctactggacc ttccacaagc cgccggagct
      901 gctggcggtc ttcatgggct acgcctcgat cgggctgggc gggatcttca tcttctgcac
      961 cgccgcctcc ttctactggc ggcgggccac cgcctccggg gccatcctct gcatggtcta
     1021 cggggtgctg tccaccctga tcggcgcgat cttcaccgcc cggggcaaga tcggcatggg
     1081 aaccctcgag ttcatcgtgc tgatcggctg cggcctctgc tacttcctcg gcagctacct
     1141 ccagaagccg ctgccggccg agaagctgaa ggagttgttc tgaggaaggg ggtttccgtc
     1201 gcggtgaaag aaaacctgaa gcggatcgcc gcggaggtgg ccgccagcct ggagaaaatc
     1261 gaacccgggc agttcgaggc ggccgcggcg gagatactga aggcgccgaa cctcttcctg
     1321 gccggcgcgg gacggagcgg ccggatggtg gaggctttcg ccatccggct ggcccagctg
     1381 ggcctgtccg cctacctggc cggcggcgcc accaccccgg ccatcaccgc cggcgacctg
     1441 ctggtaatcg gctccggctc cggccggacg gccggactgc tggccgcggc ccagaccgcc
     1501 cgggaggccg gcgcccgcct gctgatcttc accgccgagc cggaatcgcc cctggcccgg
     1561 ctggccgacc gcctggtcgt gataccggcc ggcggccgcc ggcgtgccgg ccaagggcgg
     1621 ccgaactcga tccagccgct ggcgacgctc ttcgaacaat cactgctgct gctcctcgac
     1681 ggactggtcc tgcgcctgat gaaggcgcgg cgggtctccg ccgccgccat ggccggccgt
     1741 cacgccaacc tcgaataaaa aaaggccggg ccgatacccg acgttcggcc cggcctttca
     1801 ctcaaacccc gattgaaccc gcaccatcca gatggcggtt tgatctttca gggccgccgg
     1861 aaaatcttga tttcccaggg cgaaatcttc cattcccgcg accggcggcc ggtctccgcg
     1921 tcctcccaaa cttccttgaa gaccagggtc tcctcccggt gaaagtaact ggcggccgcc
     1981 aggtagaact cctcgttgac aaaggcgctc agcaccaggt cgcgccagaa ccgaccggcc
     2041 agcaggtcct gatcctggac ctcggtgaag acgtgggtgc cgggacgggt catcttccgg
     2101 tagatgggaa agtagtggaa gtaatcctcc cggtagcggg ggttacccgg cggctgctcc
     2161 cagggcgagt agatgggttc ggccc
//
LOCUS       DAOQYN010000260         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_57396_length_2180_cov_4.862588, whole genome shotgun sequence.
ACCESSION   DAOQYN010000260 DAOQYN010000000
VERSION     DAOQYN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57396_length_2180_cov_4.862588"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PKN80_08580"
     CDS             <1..635
                     /locus_tag="PKN80_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36101.1"
                     /translation="WSAHVGDSPVKPLAGQAGEILLPILRPGARNTEFPVEVIYYLER
                     PAFGRSGDLELNLPLADIPVMQFMYSLYLPEKLKFESFGGSLEKVVSFTPIVLEDSGA
                     AGSEGKEITRRNRLQDRYLAQQRALESNVAFSDGLMSREEMMGVKPSARSGKGDSPAE
                     SAAGGAGAISGGLLPLRIYIPTTGQLLRFEKRLVIEQDLTVKAKYKQQGR"
     gene            complement(642..>2180)
                     /locus_tag="PKN80_08585"
     CDS             complement(642..>2180)
                     /locus_tag="PKN80_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HNQ36102.1"
                     /translation="RVRPQPPRPPAPDGRRTLVCLVGGDDRNYRLDPQYARELGGRLL
                     ELARTGRYRIWLTTSRRTDPASEEILTASLAGAGPEARVIGYHASRANPVREFLAEAD
                     LVLATEDSINMLSEAAASNRPTLALRVGRRRPGRLVFDRTLESMETAGHLRIVGLEDL
                     EAERLEKVLTGFRPRLLAETERAAAAVVRRLAETQVRVIGTSCLGDNLILSPAYRVLR
                     ENFPLARLEIVTDRRSAGFFEGHPYFERILYWQKQAPREEKIAFLKELRQRPADYLFD
                     FRCSLLGLLAGGRRRALFLLSQGRPRPGEHETDRWRRLLGYFLPGPAAPAFDFPALRA
                     EADWLKETLAGRGLAEKGFIILNPGGRWEKKRWPPEEFAALADLAWDRWQLPAVLVGD
                     CDETGLAAAVEKSVRRARTVNLAGATTIRQLAALLAAGRCLVSNDTGTVHLASAAGCP
                     QVVLFGPGDWRRYGPYGVPFAIVASGLRCSPCLRETCPRDFECWQKITPERVLAAAEP
                     FCAG"
BASE COUNT          328 a          731 c          813 g          308 t
ORIGIN      
        1 tctggagcgc ccacgtcggc gacagcccgg tcaagcccct ggccggccag gcgggagaaa
       61 tcctgctgcc gatcctgagg ccgggcgccc ggaataccga gtttccggtc gaggtcatct
      121 actacctgga gcggccggct ttcggacgca gcggcgacct ggaactgaac ctgcccctgg
      181 ccgacatccc ggtgatgcag ttcatgtact cgctctacct gcccgaaaaa ttgaagttcg
      241 agagtttcgg cggcagcctg gagaaggtgg tctccttcac cccgatcgtg ctggaggatt
      301 ccggcgccgc cggatcggaa ggaaaagaaa taacccgccg gaaccggttg caggaccgct
      361 acctggccca gcagcgggcg ctggagtcca acgtcgcctt ttccgacgga ttgatgagcc
      421 gtgaagaaat gatgggggtg aagccctcgg caaggagcgg caagggtgac agcccggccg
      481 aatcggcggc cgggggcgcc ggcgcgatct ccggcggcct gctgcccctg cgcatctaca
      541 tcccgaccac cggccagctg ctgcgcttcg agaagcggct ggtcatcgag caggacctga
      601 cggtgaaggc gaagtacaaa cagcagggca ggtaggcggt ctcagccggc gcagaatggc
      661 tcggcggccg ccaggacgcg ctccggggtg atcttctgcc agcactcgaa gtcgcggggg
      721 caggtctccc tgaggcaggg gctgcacctc aggccggagg cgacgatggc gaagggcacc
      781 ccgtaggggc cgtagcggcg ccagtcgccg ggtccgaaga ggaccacctg ggggcagccg
      841 gcggcgctgg ccaggtggac ggtgccggtg tcgttggaga ccaggcagcg gccggcggcc
      901 agtagcgcgg ccagctgccg gatggtggtg gcgccggcca ggttgaccgt ccgggcccgc
      961 cggaccgact tttcaaccgc ggcagccagg ccggtctcgt cgcagtcgcc caccaggacc
     1021 gccggcagct gccagcggtc ccaggccagg tcggccaggg cggcgaactc ctccggcggc
     1081 cagcgtttct tctcccagcg gccgcccggg ttgaggatga tgaagccttt ttcggccagg
     1141 ccgcggccgg ccagggtctc cttcagccag tccgcttcgg cccggagcgc cgggaagtcg
     1201 aaggcgggcg cggccggccc gggcaggaag taaccgagca ggcgccgcca gcggtcggtt
     1261 tcgtgctcgc ccggccgggg ccgcccctgg ctcagcagga aaagggcccg gcgccggccg
     1321 ccggccagca ggcccaggag cgagcagcgg aagtcgaaga ggtagtcggc cggccgctgg
     1381 cgcagctcct tcaggaaggc tatcttctcc tcacgaggcg cctgcttctg ccagtagagg
     1441 atccgctcga agtagggatg cccttcgaag aagccggccg agcggcggtc ggtgacgatc
     1501 tccagccggg cgagtggaaa gttctcgcgc aggacccggt aggcgggcga gaggatcagg
     1561 ttgtcgccca ggcagctggt cccgatgacc cgcacctgcg tttcggccag gcgtcggacc
     1621 accgccgcgg ccgcccgctc cgtctcggcc agcaggcggg gccggaaacc cgtaagcacc
     1681 ttctccagcc tttccgcttc cagatcctcc aggccgacga ttctcaggtg gccggcggtt
     1741 tccatcgact ccagggtgcg gtcgaagacc agccggcccg gccggcgccg gccgacgcgc
     1801 agggccaggg tgggccggtt cgaggcggcc gcctcggaga gcatgttgat gctgtcctcg
     1861 gtggccagga ccaggtcggc ttcggccagg aactcccgga ccgggttggc ccggctggcg
     1921 tggtagccga tcacccgcgc ctccgggccg gctccggcca ggctggcggt caggatctct
     1981 tcgctggccg ggtcggtgcg gcggctggtg gtcagccata tccggtagcg gccggtccgc
     2041 gccagttcca ggaggcggcc gcccagttcc cgggcgtatt gcggatcgag ccggtagttg
     2101 cggtcgtcgc cgccgaccag gcagaccagg gtgcggcgtc cgtccggggc cggcggccgg
     2161 ggcggctggg ggcggacccg
//
LOCUS       DAOQYN010000261         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_58538_length_2154_cov_5.251548, whole genome shotgun sequence.
ACCESSION   DAOQYN010000261 DAOQYN010000000
VERSION     DAOQYN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58538_length_2154_cov_5.251548"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2154
                     /locus_tag="PKN80_08590"
     CDS             <1..>2154
                     /locus_tag="PKN80_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36103.1"
                     /translation="AGGRIISRPLAEPVPAGKYRLFLRVIGPTSPDRESIVRVGLGAS
                     RVEVAWKTGRKNFTWLPGVEITLAAPAASISLEAVQFGGRGYGAYTEPLNRSLWVDTI
                     YLSDDPNEKEPPDYHQETARRAGLDTAALAPRPVYQSDEPYRTPAPAAEPVFGPIKLQ
                     SFDGRRNLWPNSSFELGMNDGWAGKSAYAFTDADLDNVRPFHGLYSLKVPAVEPFSRP
                     YYLEQAGIYTISLYVRSEKPADFGFVLKKVIEDGSWQKNEIHTLNALKVIGPAGPEWR
                     RLSATGNLEPGWYYLSLSGSGWVDGVQLEAGAAATEYAPRAELEGALRSGCLGNIIYD
                     NGPLTLDLWFHNSGRTSRTARLGYRVVDVRERVVAEGETEPVSLAGGQTVRRAVAIRP
                     PLRGIFSATYGVSGRELPEGETVYLVMSRPPEGPVRHKLGDNLTLNEAEILVHKRLGM
                     SWVMPAKVRELGALTEPPDGGVHPAPEVWNWQDERAALPRKHGLEMLSCFWIFRRPPD
                     FMLDPVDPDQYRTTRGMTREYQPKLDLWADYAGRVAKHYRSYVRGWFVDDQAENHWDP
                     KLFESVAGAAADAIHREAPGVWVGISATPEFTEELMRYLPPEKIDFFSNDALNYNYHE
                     SRYYAGFKRRYQKPLFCGGVGGYPPEETMYHTLYTYQPGRFKAAWMARQLIYHTLVMD
                     LDVIGYYAGVLANYGAHLGQNKPLCDYDATPYPWG"
BASE COUNT          357 a          716 c          756 g          325 t
ORIGIN      
        1 cggccggcgg ccggatcatc agccggccgc tggcggagcc ggtcccggcc ggcaagtacc
       61 gcctcttcct gcgggtgatc ggcccgacca gccccgaccg ggagagcatc gtccgggtgg
      121 gcctgggcgc cagccgggtc gaggtcgcct ggaagaccgg ccggaagaac ttcacctggc
      181 tgccgggggt ggagatcacg ctggcggcgc cggccgcgag tatcagcctg gaggcggtcc
      241 aattcggcgg ccgcggctac ggcgcctaca ccgagccgct gaaccggagc ctctgggtgg
      301 acaccatcta cctgagcgac gacccgaacg agaaggagcc gcccgattac caccaggaga
      361 cggcccggcg ggccggcctg gacacggcgg ccctggcgcc gcggccggtt taccagtccg
      421 acgagcccta ccggacgccg gccccggcgg cggagccggt cttcggcccg atcaaattac
      481 agtccttcga cggccggcgg aacctctggc cgaactcctc ctttgaactg gggatgaacg
      541 acggctgggc gggcaagtcg gcctacgcct tcacggatgc cgacctggac aatgtccgtc
      601 cctttcacgg cctctactcg ctgaaggtcc cggccgtgga gcccttcagc cgcccctact
      661 atctcgagca ggccggtatc tacaccattt ccctgtatgt ccgctccgag aagccggccg
      721 atttcggctt cgtcctgaag aaggtaatcg aggacgggag ctggcagaag aacgagatcc
      781 acaccctcaa cgcgcttaag gtcatcggcc cggcgggtcc ggagtggcgg cgcctgagcg
      841 cgaccggtaa cctggagccc ggctggtact acctgagcct gagcgggagc ggctgggtgg
      901 acggggtgca gctggaggcg ggtgcggcgg cgacggagta cgcgccgcgg gccgagctgg
      961 agggggccct gcgcagcggc tgcctgggga acatcattta cgataacggg ccgctgacgc
     1021 tggacctctg gttccacaac agcggccgga cgagccggac ggcgcgcctg ggctaccggg
     1081 tggtggacgt gcgggagcgg gtggtggccg agggcgagac cgagccggtg tcgctggccg
     1141 gcggccagac ggtgcggcgg gcggtggcga tccggccgcc gctgcggggg atcttctcgg
     1201 cgacttacgg ggtctccggc cgggagttgc cggagggcga gacggtttat ctggtgatga
     1261 gccggccgcc ggaaggtccc gtccgccaca agctgggtga caacctgacc ctgaacgagg
     1321 ccgagatcct cgtccacaag cggttgggaa tgagctgggt gatgccggcc aaggtgcgcg
     1381 aactgggcgc ccttaccgag ccgccggacg gcggcgtcca cccggcgccg gaggtctgga
     1441 actggcagga cgagcgggcc gccctgccca ggaagcacgg cctcgagatg ctctcctgct
     1501 tctggatctt ccggcggccg ccggatttca tgctcgaccc ggttgatccg gaccagtacc
     1561 ggaccacccg cggcatgacc agggagtacc agccgaaact ggacctctgg gccgattacg
     1621 ccggccgggt ggccaaacac taccggtcct acgtgcgggg ctggttcgtg gacgaccagg
     1681 ccgagaatca ctgggacccg aagctcttcg agtcggtcgc cggcgcggcc gccgacgcca
     1741 tacatcggga ggcgcccggg gtctgggtgg gtatcagcgc caccccggag ttcaccgagg
     1801 aactgatgcg ttatctgccg ccggagaaga tcgacttctt cagcaacgac gccctgaatt
     1861 acaattacca cgagtcccgc tactacgccg gtttcaagcg gcgctaccag aaacccctct
     1921 tctgcggcgg ggtgggcggc tacccgcccg aagaaacgat gtaccacacc ctttacactt
     1981 accagccggg ccgtttcaag gcggcctgga tggcccgcca gctgatttac cacaccctgg
     2041 tcatggacct tgatgtcatc ggttactacg ccggggtgct ggccaattac ggggcccacc
     2101 tgggccagaa caagccgctc tgcgattacg acgccacgcc ctatccctgg ggca
//
LOCUS       DAOQYN010000262         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_59237_length_2138_cov_4.660586, whole genome shotgun sequence.
ACCESSION   DAOQYN010000262 DAOQYN010000000
VERSION     DAOQYN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59237_length_2138_cov_4.660586"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PKN80_08595"
     CDS             complement(<1..696)
                     /locus_tag="PKN80_08595"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HNQ36104.1"
                     /translation="MSEDKYHREISAPAWSNPPLEDHKGHRLMEVGDAAFFKKGLKVF
                     FGDVHIHTNYSRCGFPNNGNIAENLAYAREESRLDFAAVADHAEHLEPKQYPGYCREV
                     EAGNDPGRFVTLPAFEWASHTLPTNGHRVVYFRERFGPLMGGKDPRYDTPRKLALALR
                     RIRPGVIAPRHHPTYVNDWRSVDPDFEPVIEIHSEWGCSERDGGPLSPSPGRWPVWPG
                     NYAQDGLARGHRFG"
     gene            complement(689..>2138)
                     /locus_tag="PKN80_08600"
     CDS             complement(689..>2138)
                     /locus_tag="PKN80_08600"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNQ36105.1"
                     /translation="DSVRLLQKRLDGRGRPLVLVDHGWQAAGGTLGRHEGSDRRFPGG
                     MKRLAGEIRALGGRPAIWFSPVRYNGASPFLKAHPEAALREARSDEALKYRERPDENG
                     RCSYFLDPTHPQTRREVVRCLERFAGWGYDYLKADFVEIPDWFAYRHHDRRTAANGPV
                     NRALFETIYPAARRLGMRVLACITFIDRLDGLKDDQRVAHDTNPAAGPDHLAAMATTV
                     FSRYFYHRRLQHNDPDCLCLTGRPPKKPELSEGEARLRTLVVFLSGGTILLGDDPALV
                     PEERFDLFRRCLPVFGRTGRPLDLFEAGGAPAVHRLEVPDYGDGRVLTAFTNFTGRPR
                     SFRLEAAENGKPAAGLELFSGREFRLEAGESSVTVPPLDAALYLLVPELEHPWLTGTD
                     RHWTGGFKGVVDRLAWDPARRTLRFRFRNLFNHPAARFWIQWPAGYRPAGLKTSGAAA
                     AWEAGGVLRLARPSISENIQISFKTGEVQKDV"
BASE COUNT          300 a          699 c          805 g          334 t
ORIGIN      
        1 gccgaagcgg tggccgcggg ccaggccgtc ctgggcatag ttgcccggcc agaccggcca
       61 gcggccgggc gagggcgaga gcgggccgcc gtcccgctcg ctgcagcccc actcggagtg
      121 gatttcgatc accggctcga agtccgggtc aacgctccgc cagtcattga cgtaggtcgg
      181 gtggtggcgc ggggcgatca cccccggccg gatccggcgc agggccaggg ccagcttgcg
      241 cggggtgtcg tagcgggggt ccttgccgcc catgagcggc ccgaagcgct cccggaagta
      301 gacgacccgg tggccgttgg tcggcagggt gtggctggcc cattcgaagg ccggcagggt
      361 gacgaagcgg cccgggtcgt tgccggcctc cacctcccgg cagtagcccg ggtactgctt
      421 cggctccagg tgctcggcgt ggtcggctac ggcggcgaag tccagccggc tctcctcgcg
      481 ggcgtaggcc aggttttcgg cgatattgcc gttgttcggg aagccgcagc gggagtagtt
      541 ggtatggatg tggacatcgc cgaagaaaac cttgagcccc ttcttgaaga aggcggcgtc
      601 gccgacctcc atcagccggt gtcccttgtg atcctccagc ggcgggttcg accaggccgg
      661 cgccgaaatt tcgcggtggt acttatcttc agacatcttt ttgaacctcc ccggttttga
      721 atgatatttg aatgttttcg ctgatcgagg gccgcgccag ccggagcact ccgccggcct
      781 cccaggccgc cgcggcgccg gaggtcttca gcccggccgg ccggtatccg gccggccact
      841 ggatccagaa gcgggcggcc ggatggttga aaaggttgcg gaagcggaac cggagcgtgc
      901 ggcgcgccgg atcccaggcc agccggtcaa ccacgccctt gaagccgccg gtccagtggc
      961 ggtcggtgcc ggtcagccag gggtgttcga gttccggcac cagcaggtag agggcggcgt
     1021 ccaggggcgg cacggtcacg gaactttccc cggcttcgag gcggaattcc cggccggaga
     1081 agagttccag gccggcggcc ggttttccgt tttccgccgc ctccaggcgg aagctgcgcg
     1141 gccggccggt gaagttggtg aaggcggtca ggacgcggcc gtcgccgtaa tcgggcactt
     1201 ccagccggtg taccgccggc gcgccgccgg cctcgaagag gtccagcggc cggccggtcc
     1261 ggccgaagac cggcaggcag cgccggaaca ggtcgaagcg ttcctccggc accagggccg
     1321 gatcgtcgcc gagcaggatg gtgccgccgg agaggaagac gaccagggtg cgcagccggg
     1381 cctccccctc gctcagctcc ggtttcttcg gcggccggcc ggtcaggcag aggcagtccg
     1441 ggtcattgtg ctggaggcgc cggtggtaga agtagcggga gaagacggtg gttgccatcg
     1501 cggccaggtg gtcggggccg gccgccgggt tggtgtcgtg ggcgacccgc tggtcatcct
     1561 tcagcccgtc caggcggtcg atgaaggtga tgcaggccag cacccgcatt cccagccgcc
     1621 gggccgccgg gtagatcgtt tcgaagaggg cccggttgac cgggccgttg gccgccgtcc
     1681 ggcggtcgtg gtggcggtag gcgaaccagt caggtatctc gacaaagtcc gccttcaggt
     1741 agtcgtagcc ccagccggcg aagcgctcca ggcaacggac cacctcgcgc cgggtctgcg
     1801 ggtgggtcgg gtccaggaaa taggagcagc ggccgttctc gtcgggccgt tcccggtact
     1861 tcagggcttc gtccgaccgc gcttcgcgca gggctgcctc cgggtgggcc ttgagaaagg
     1921 gagacgcccc gttgtagcgg accggtgaga accagatggc cggccggccc ccaagcgccc
     1981 gtatttcgcc ggccagtcgc ttcatgccgc ccgggaagcg gcggtcgctc ccctcgtggc
     2041 ggccgagggt gcccccggcc gcctgccagc cgtggtcgac caggaccagg gggcggcccc
     2101 ggccgtcgag ccgcttctgc aggaggcgga ccgagtcg
//
LOCUS       DAOQYN010000263         2136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_59332_length_2136_cov_3.552138, whole genome shotgun sequence.
ACCESSION   DAOQYN010000263 DAOQYN010000000
VERSION     DAOQYN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59332_length_2136_cov_3.552138"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PKN80_08605"
     CDS             <1..679
                     /locus_tag="PKN80_08605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNQ36106.1"
                     /translation="SLLVVPLRIRSTNVGLIAVLGKETNAFTGSDLEFMSTLAGQVSL
                     IIENATLFEKTRRLSMTDGLTNLFNRRYFEEFLDQEIRKAEKDVTPLSIAMIDLDDFK
                     KFNDRNGHLAGDEALRCIARTLRQGTKGRDIIARYGGEEFVIIFPQTLPANAFLVCEN
                     LRRAVERQPFPGETLQPGCNLTISIGLAAYPDDGQNSRLVLEKADARLYEAKRLGRNR
                     VVIADAG"
     gene            complement(695..1018)
                     /locus_tag="PKN80_08610"
     CDS             complement(695..1018)
                     /locus_tag="PKN80_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36107.1"
                     /translation="MLKKTIETIQETERQAEALVKEAAGEKARLLEEVRKRRPEFESA
                     GDREAASEINKLVGAFDARRQQELEGLKTGLDERRTELSRHFEANRKAALDCLLKVLT
                     TRPRK"
     gene            complement(942..>2136)
                     /locus_tag="PKN80_08615"
     CDS             complement(942..>2136)
                     /locus_tag="PKN80_08615"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNQ36108.1"
                     /translation="KPGATGRLLVSLGESSRILTAGDRVRVRLDPIAAGRNFSTEYRK
                     TLGRRGIYTLARVVPADFSLLGETETTTRSGWPARMRQSIRRIFERQIPDEPQRSIMA
                     AVSFGLQRPAAPLRHGFQRLGIYHLLVVSGLHVAYFNYFFSLLLLPFRPGLRLRSMLH
                     LPALAFYAMLCGFSAPVLRATLMAGFHHLGIALNRESRPLNAISLAAFVLLVRDPGQL
                     FEPGWQLTFVITGALVLGASRCRNLFGKLPGWLAGTLGASLAGQAAGLPLSALYFGQV
                     PLLGLIVNPFFIPLAGLLVLESFLMIAAPWGRELFAAAGGLLSLIFVELSRRLAEWPL
                     ASLQVPRGNPAYLLIYLPLAALLILNREKDKIKTFGVQNVKEDDRNDSGDGAAGRSPG
                     KRGRR"
BASE COUNT          468 a          664 c          627 g          377 t
ORIGIN      
        1 ctccctgctg gtggtccctc tgcgcatccg cagcacgaat gtcggcctga tcgccgtgct
       61 gggcaaggaa accaacgcct tcaccggcag cgatctggaa ttcatgagca cgctggccgg
      121 acaggtaagc ctgattattg aaaacgccac cctcttcgag aagacccgcc gcctctccat
      181 gaccgacgga ttaaccaatc ttttcaaccg ccgttatttc gaggaattcc tggaccagga
      241 gatccggaag gcggagaagg acgtcacccc gctttcaatc gccatgatcg acctggacga
      301 tttcaagaaa ttcaacgacc ggaacggtca cctggccggg gacgaagcct tgaggtgcat
      361 cgctcggacg cttcgccagg gcaccaaggg tcgggatatc atcgcccgtt acggcgggga
      421 ggagtttgta ataatctttc cccagaccct gccggccaac gcctttttgg tctgcgaaaa
      481 tctgcgccgg gccgttgaaa ggcagccttt cccgggcgaa accttgcaac ccggctgcaa
      541 ccttaccatc agtatcggac tggccgccta tccggacgac ggccagaatt ccaggctggt
      601 tctcgaaaaa gccgacgccc gcctttatga agccaaacgg ttgggccgca accgggtggt
      661 aatagccgac gcggggtaga aacggggagg ggccttattt cctggggcgg gtggtcagga
      721 ccttcagcag gcagtcgaga gcagccttcc gatttgcttc aaaatgacgg ctcaactccg
      781 tccggcgctc gtccaggccg gttttcaatc cttccaattc ctgctgccgc ctggcgtcaa
      841 aagcaccgac gagcttgttg atttcagacg cggcctcccg gtctccggcc gattcaaact
      901 ccggccggcg cttccgaacc tcttccagga gccgggcctt ttcaccggcg gcctctttta
      961 ccagggcttc ggcctgccgc tccgtctcct gaatcgtttc gatcgtcttc tttaacattt
     1021 tggactccga acgtttttat tttatccttt tcccggttta aaatcaaaag ggccgccagc
     1081 ggaagataaa tcaacaggta agccgggttc cctcgcggaa cctggaggga agccagcggc
     1141 cattccgcca ggcgccttga aagctccacg aaaatcagcg atagcagacc gccggcggcg
     1201 gcgaacaatt cccgccccca gggcgccgcg atcatcagaa aggattccag gaccagcaac
     1261 ccggccagcg gaatgaaaaa cgggtttacg atcaaaccca gcaggggcac ctggccgaaa
     1321 taaagggcgc tcaagggaag cccggccgcc tggccggcca aactggcacc cagcgtgccg
     1381 gccagccagc cgggcaattt tccaaaaagg ttccggcacc gggaggcgcc cagcaccagg
     1441 gccccggtaa tgacaaaagt aagctgccag ccgggttcaa agagctggcc gggatcccgg
     1501 accaggagca caaaagcggc caggctgatg gcgttaagcg gccgcgattc ccggttgagc
     1561 gcaattccca ggtggtgaaa accggccatc agcgttgcac gcagcaccgg ggccgaaaaa
     1621 ccgcagagca ttgcataaaa ggccagggcc ggcaggtgca gcatggaacg cagtcggagc
     1681 ccgggtcgaa acggaagcag cagcaggctg aaaaaatagt tgaaataggc gacatgcagg
     1741 ccggaaacaa ccagcagatg atagatgccc aggcgttgaa aaccatggcg taacggtgcg
     1801 gccggccgct gcaatccgaa ggagaccgcc gccattatgg aacgctgcgg ttcgtccggg
     1861 atctggcgtt caaagatgcg ccggatgctc tggcgcatcc tggccggcca accggaccgg
     1921 gtcgtcgttt ccgtttcgcc cagcagcgaa aaatcggccg ggaccacccg ggccagggtg
     1981 taaatgcccc tccgacccag cgtttttcgg tactcggttg aaaaattccg gccggccgcg
     2041 atcggatcca ggcgtacccg gacccggtca ccggcggtca gaattcggct tgattccccc
     2101 aggctgacca gcaggcgtcc ggttgctccc ggtttc
//
LOCUS       DAOQYN010000264         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_59571_length_2131_cov_4.028420, whole genome shotgun sequence.
ACCESSION   DAOQYN010000264 DAOQYN010000000
VERSION     DAOQYN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59571_length_2131_cov_4.028420"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PKN80_08620"
     CDS             <1..615
                     /locus_tag="PKN80_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hexulose-6-phosphate
                     synthase/ribonuclease regulator"
                     /protein_id="HNQ36109.1"
                     /translation="GLLEVLRKVSTANLSDALHRAAGLEGIRPINTWKGLKMAGPALT
                     VRTYPGDWAKPVEAVDQAEPGQVIVIEAGGRTPAVWGELATSSAVVKKLAGVVIDGAI
                     RDTSDIAGLKFPAFSRLICPQAGEPKGLGEIGVPITVGGQRVMTGDWVAGDADGVMVI
                     PRAEAVEFANRAMDVLEKENRLREEIQRGGTLAQITELLKWEKR"
     gene            675..1001
                     /locus_tag="PKN80_08625"
     CDS             675..1001
                     /locus_tag="PKN80_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36110.1"
                     /translation="MDIKGRMVKVRFKKYFAEQRLWLFLGRVLEISENWIQVEGRGIV
                     HFHAPRRTEIDEEPRVVVIPRDNIAHIRILPDSFTLDKIEILTRGGRFFVKVPDGPDT
                     SIGDEF"
     gene            complement(998..>2131)
                     /locus_tag="PKN80_08630"
     CDS             complement(998..>2131)
                     /locus_tag="PKN80_08630"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNQ36111.1"
                     /translation="YRPKGDGPFPALALVRGRTRSGGEYYGPFTDVWALRRSLRNLRR
                     VFPVAACRRRIDPARPVRPCLDHSLGRCLAPCAGLVTAAEYDRTVRALRAFFQGRNPA
                     LVRELKSAMAEASRRLEFERAARLRDELEALQRVARHPRGLSRKFGRELPADRRVLKL
                     KERLGLEKEPLRIEAYDISNLFGSEAVGSMVTFLAGSPYRDGYRRFRIRGVRGSDDYA
                     MLAEVLRRRFSETGWPKPDLVLVDGGRGQVETARRVMKEVGVDLPLVGLAKKFERFFV
                     AGRRLPLPVPPESPEGLFLRQVRDEAHRFAVGYHRRLRSRKLTASWLDEIPGLGPVNR
                     ARLRRAFPSAAAIRAATVEDLAAAGIRPELARRVKEAAGAGPG"
BASE COUNT          374 a          742 c          729 g          286 t
ORIGIN      
        1 gggctgctcg aggtcctcag gaaggtctcc accgccaacc tttccgacgc cctccaccgg
       61 gcggccggtc tggaaggcat ccggccaatc aacacctgga aggggttgaa gatggccggc
      121 ccggcactga cggtgcgcac ctatcccggt gactgggcca agccggtcga ggcggtcgac
      181 caggccgagc cgggccaggt gatcgtcatc gaggccggcg gccgcacccc ggcggtctgg
      241 ggcgaactgg ccaccagcag cgccgtcgtg aagaaactgg ccggagtggt catcgacggc
      301 gccatccgcg acaccagcga catcgccggg ctcaagttcc cggctttctc gcgcctgatc
      361 tgcccccagg ccggcgaacc gaagggtctg ggcgagatcg gcgttccgat caccgttggc
      421 ggccagcggg tgatgaccgg ggactgggtg gcgggcgacg ccgacggggt gatggtcatt
      481 cccagggccg aggcggtgga gttcgccaac cgggcgatgg acgtgctgga gaaggagaac
      541 cggctgcgcg aggagatcca gcgcggcggc accctggccc agatcaccga actgctcaag
      601 tgggaaaaac gttgactgca gccggttccg ggcactccaa aggtccggct caaccttaaa
      661 gaaaaggaga cggaatggat atcaagggca ggatggtaaa ggttcgcttc aagaaatact
      721 tcgccgagca gcgcctctgg ctcttccttg gtcgggtgct cgaaatttcg gaaaactgga
      781 tccaggtcga gggacgggga atcgtgcact ttcacgcgcc gcgccggacc gagatcgacg
      841 aggagccccg ggtagtggtc atcccccgcg acaatatcgc ccacatccgg atcctgcccg
      901 acagcttcac tctcgacaaa atcgaaatac tcacccgggg cggccgcttc ttcgtgaagg
      961 tcccggacgg accggacacc agcatcggcg acgaattcta accgggcccg gcaccggccg
     1021 cctctttgac ccgccgggcc agttccggcc ggattccggc cgcggccagg tcctcgacgg
     1081 tcgccgcccg gatggcggcc gccgatggga aggcgcgccg cagccgggcg cggttgaccg
     1141 gacccaggcc ggggatctca tccagccagg aggcggtcag cttccggctg cgcagccgcc
     1201 ggtgataacc gaccgcgaac cgatgcgcct catcgcgcac ctgccttaaa aaaagccctt
     1261 cgggcgactc cggcggcacc ggcagcggca ggcggcgtcc ggccacgaag aaccgctcga
     1321 acttcttggc caggccgacc aggggcaggt cgaccccgac ctccttcatc acccggcggg
     1381 ccgtctcgac ctggccgcgg cccccatcga ccagcaccag gtccggcttc ggccagcccg
     1441 tctccgaaaa ccgccgccgc aaaacctccg ccagcatggc gtaatcatcg ctgccccgga
     1501 cgccgcggat ccggaaacgc cggtaaccgt cgcggtacgg ggacccggcc aggaaggtca
     1561 ccatcgatcc caccgcctcc gaaccgaaga ggttggagat gtcgtaggcc tcgatccgca
     1621 aaggttcctt ctccagcccc agccgttcct tgagtttgag cacccgccgg tcggccggca
     1681 gctcccggcc gaatttccgg ctcagtccgc gcggatggcg ggccacccgc tgcaaggcct
     1741 ccagctcgtc ccgcaggcgg gccgcccgct cgaattccag ccggcgggag gcctcggcca
     1801 tggccgactt caattcccgg accagcgccg ggttgcgccc ctggaagaag gcccgcaggg
     1861 cccggacggt ccggtcgtat tcggccgcgg tgaccaggcc ggcgcagggc gccaggcagc
     1921 ggcccaggct gtggtcaagg cagggccgga ccggccgggc cggatcgatg cggcggcggc
     1981 aggcggcgac cggaaagacc cggcgcaggt tgcgcagcga gcggcgcagg gcccagacat
     2041 cggtgaaggg tccgtaatat tcgccgccgg accgggtccg cccgcgcacc agcgccaggg
     2101 cgggaaaggg cccgtcgccc ttgggacggt a
//
LOCUS       DAOQYN010000265         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_60012_length_2121_cov_3.985963, whole genome shotgun sequence.
ACCESSION   DAOQYN010000265 DAOQYN010000000
VERSION     DAOQYN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60012_length_2121_cov_3.985963"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..535
                     /locus_tag="PKN80_08635"
     CDS             119..535
                     /locus_tag="PKN80_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNQ36112.1"
                     /translation="MDFLTARERLGVILITGSLLVGQAVLTFQNRNGVHYRYLESDAR
                     RLAELENRQRGTARVRMLERDLQARIDPNTATAEELETLPGIGPALSRRIITHRSRRP
                     FRTLDELKEVPGIGPAKLGALKARLEIRTPGTKADR"
     gene            537..938
                     /locus_tag="PKN80_08640"
     CDS             537..938
                     /locus_tag="PKN80_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNQ36113.1"
                     /translation="MYIIIVGCSRVGIDLAQALDSEGHNVVVIDRNQESLARLGRSFS
                     GLLISGNGYEPEVLREAGIEKADALLAVTDNDNTNLVTTQVARKLFGKEKVLARIYDP
                     VRAETFQKMAVDTVSETTLVSEAFRKLLFDK"
     gene            939..1601
                     /locus_tag="PKN80_08645"
     CDS             939..1601
                     /locus_tag="PKN80_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNQ36114.1"
                     /translation="MYVIIIGGGNVGYYLAHRLVRDNHTVALVDKDRATCDRIGQELD
                     ILVINGDGCELHTLDAAGIERADVLAAVTGSDEDNLIVCQLAKELFRVDRTVARVNDS
                     RNEHIFNELGVDVPIDSTGILARVIEEEVSLSDIVNLVTFRKGKLALVRVDLPDNSSA
                     VGKQVSEIPLPKESVLVALIRGSEVFIPHGNTILKSQDEIIALTRLGDENSILKVFLP
                     EI"
     gene            1609..1773
                     /locus_tag="PKN80_08650"
     CDS             1609..1773
                     /locus_tag="PKN80_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36115.1"
                     /translation="MDLKKLLPRRLRNALASFENGVIGIAYTMLLPLLLAAALLSILV
                     LAVAFGNLIR"
     gene            1789..>2121
                     /locus_tag="PKN80_08655"
     CDS             1789..>2121
                     /locus_tag="PKN80_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNQ36116.1"
                     /translation="MFLKPRKEDFQIIGYYLGKLSAGLGIAMLLPLAAAFYYREWNAA
                     LDFIVGATIALILGRLLTLVRPRKTDLSWANGVVIAGLSWLVAMFLAAVPLYLSGHYQ
                     AFVDALFET"
BASE COUNT          423 a          700 c          631 g          367 t
ORIGIN      
        1 acacggcgag gctcgttgcc gagcggcgta gttttgtcca gcgcgcagca ccgcgacgca
       61 aaacgaagac cgccgcgctt gacttttgga agccgaagtt caataatata cctcataaat
      121 ggacttcctg actgccagag aacggctggg cgtcattctc atcaccggca gcctcctggt
      181 cgggcaggcg gtcctgactt tccagaatcg caacggtgtc cattaccggt acctggagtc
      241 cgatgcccgc cggctggcgg agctggaaaa ccggcagcgc gggactgcgc gcgtccggat
      301 gctggaaaga gacctccagg cccgcatcga ccccaacacc gccacggccg aagaactcga
      361 aaccctgccg ggcatcggac cggcactgag ccggcgcatc atcacccatc gaagccgccg
      421 gccgtttcga acgctggacg aacttaaaga agttccgggt atcggtcccg caaaactggg
      481 agccctgaaa gccaggctgg agatccggac gccgggaacg aaagcggatc gataaaatgt
      541 acatcatcat cgtgggatgc agccgggtgg gtatcgacct ggcccaggcg ctggacagcg
      601 aggggcataa cgtggtggtc atcgaccgca accaggagag cctcgcccgg ctcggccggt
      661 ccttcagcgg cctgctgatc tccggcaacg gttacgaacc ggaggttcta agggaggcgg
      721 gcatcgaaaa ggcggatgcc ctgctggcgg taaccgacaa cgacaacacc aacctggtca
      781 ccacccaggt cgcccgcaaa ctattcggca aggagaaggt cctggcccgg atctacgacc
      841 cggttcgcgc cgagaccttt cagaagatgg cggtcgatac cgtcagcgag accacgctgg
      901 tctcggaggc ctttcgcaaa ctgctcttcg acaaataaat gtacgtgatc atcatcgggg
      961 gcggaaacgt cggctactac ctggcccacc ggctggtgcg cgataatcat accgtggccc
     1021 tggtggacaa ggaccgggcc acctgcgacc ggatcggcca ggaactggac atcctggtca
     1081 tcaatggcga cggctgcgag ttgcataccc tggacgcggc cggaatcgaa cgggccgacg
     1141 tgctggccgc ggtgaccggc agcgacgagg ataacctgat cgtctgccag ctggccaagg
     1201 aactcttccg ggtggaccgg accgtggccc gggtcaacga cagccgcaac gaacacatct
     1261 tcaacgaact gggcgtcgac gtccccatcg acagcaccgg catcctggcc cgggtgatcg
     1321 aggaggaggt ctccctctcg gacatcgtca acctggtcac cttccgcaag ggaaaactgg
     1381 ccctggtccg ggtcgacctc cccgacaact cctccgcggt cggcaagcag gtatcggaga
     1441 tcccgctgcc caaggagtcc gtgctggtgg cgctgatccg gggcagcgag gtcttcatcc
     1501 cccacggcaa caccattctc aagtcccagg acgaaataat cgccctgacc cgcctgggcg
     1561 acgagaattc catactcaag gtcttcctgc ccgaaatctg aagccagcat ggacctgaag
     1621 aaactgctgc cccgccggct gcgcaacgcc ctggcatcct ttgaaaacgg ggtgatcggg
     1681 atcgcctaca ccatgctgct gccgctcctg ctggcggcgg ccctgctctc catactcgtc
     1741 ctggccgtgg ccttcggcaa cctgattcgt taaccggccg ctgacgacat gttcctgaaa
     1801 ccgcgcaagg aagatttcca gatcatcggt tactacctgg gcaaactctc ggccgggttg
     1861 ggcatcgcca tgcttttacc cctggcggcc gccttttatt accgggagtg gaacgccgct
     1921 ctcgacttca ttgtgggggc gaccatcgcc ctgatcctgg gccgcctgct gaccctggtc
     1981 cgcccccgga agaccgatct ctcctgggcc aacggcgtgg tgatcgccgg actctcctgg
     2041 ctggtcgcca tgtttctcgc cgccgtcccg ctctacctgt ccggccacta ccaggccttc
     2101 gtcgacgccc tcttcgagac c
//
LOCUS       DAOQYN010000266         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_60412_length_2112_cov_4.696646, whole genome shotgun sequence.
ACCESSION   DAOQYN010000266 DAOQYN010000000
VERSION     DAOQYN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60412_length_2112_cov_4.696646"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PKN80_08660"
     CDS             <1..588
                     /locus_tag="PKN80_08660"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HNQ36117.1"
                     /translation="PHLVTFLDSPMAISVTGIFKKYPEYFDEATKKIIRTGHSPFDFP
                     LLNQTRTAAASKAINHIRGSAIIMAGSGMCNGGRIKHHLVSNIERPESTIVFVGYQAR
                     GTLGRQILERPGSVRILGQNYQVRAKVEQLDGLSAHADREELLRWLDGFQKGPDRIFL
                     VHGEEESTADFGKLLKERKPGAEVITPAFLGEHEL"
     gene            737..2086
                     /gene="lpdA"
                     /locus_tag="PKN80_08665"
     CDS             737..2086
                     /gene="lpdA"
                     /locus_tag="PKN80_08665"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNQ36118.1"
                     /translation="MEKYDLVILGGGPGGYLSAIRAAQRGLKTALVEKKYLGGTCLNW
                     GCIPTKCLADVAHTMEKARRAAAQGVDFGTPAVDLPRLIGWKDRVVAGLRSGIGYLLK
                     SHGVTVVNAEGRLAAADAIQAGESRLRAGCIILATGSVPARPGFLPIGDGRYLTSDEL
                     LSLERLPESLAVVGGGAIGVEFASIFASFGTRVAVYEMLPRLLPLEDPEVGRTIERAF
                     KKRGIAVQTGRPAERAELDRFQLVLVAIGRQPVTAGLGLEEAGVALDGKGFVRTGEDL
                     QTSRPGIYAIGDIAGRGQLAYLAGEQGISVVEGILGNRRPVACEFLPSCVFTQPEVGT
                     FGVTEEQAGREPGRYRIGRFPFTALGKAHCLGETEGFVKLVAETATGRIAGGQIVGPG
                     AADLVHLASLAAASEGTVETVAEAIFGHPTLAESVKEAAADILGLASHLPARKAAGA"
BASE COUNT          354 a          732 c          705 g          321 t
ORIGIN      
        1 ccgcacctgg tcaccttcct ggacagcccg atggcgataa gcgtgaccgg gatcttcaag
       61 aagtatcccg agtacttcga cgaggcgacc aaaaaaatca tccggaccgg ccattcgcct
      121 tttgatttcc cgctgctgaa ccagacccgg acggcggccg cctcgaaagc aatcaatcac
      181 atccgggggt cggccatcat catggccggt tcgggcatgt gcaacggcgg ccggatcaag
      241 caccacctgg tctccaacat cgaacggccg gaatcgacca tcgtcttcgt cggctaccag
      301 gcccggggca cgctcggccg gcaaatactc gagcggcccg gctcggtaag gatcctgggg
      361 cagaattacc aggtcagggc gaaggtggag cagctcgacg ggttatcggc ccatgccgac
      421 cgggaggagc tcctgaggtg gctggacggc ttccagaaag gcccggaccg gatcttcctc
      481 gtccacggcg aagaggagtc caccgccgat ttcggcaagc tgctcaagga aagaaaaccc
      541 ggcgccgagg ttatcacccc ggccttcctg ggcgaacacg aactctgaaa cccgggcccc
      601 ggcggccggc cggtcttggc cggccggcgg ccgccgcgtt ataatatccg gggttcccct
      661 ttattaagta gttaaatctt ttttagataa tggcccgacc tcggtcgcgg ttcccaaccg
      721 gttaaggatc aaacctatgg aaaagtatga cctggtcatc ctgggcggcg gccccggcgg
      781 ctacctgtcg gccatccggg ccgcccagcg cggactgaag accgccctgg tcgagaagaa
      841 gtacctgggc ggcacctgcc tcaactgggg ctgcatcccg accaagtgcc tggccgatgt
      901 cgcccatacc atggagaagg cccggcgggc ggcggcccag ggcgttgatt tcgggacccc
      961 ggccgtggac ctaccccggc tgatcggctg gaaggaccgg gtggtggccg gactgcgctc
     1021 cggcatcggc tatctcctga agagccacgg cgtaaccgtg gtgaatgccg agggacgcct
     1081 ggccgccgcc gatgccatcc aggcgggtga gtcccggctc cgggccggct gcatcatcct
     1141 ggccaccggc tcggtgccgg cccggcccgg cttcctgccg atcggcgacg gccgctacct
     1201 gacctccgac gaactcctct ccctcgaacg cctgccggaa tcgctggccg tcgtcggcgg
     1261 cggcgcgatc ggggtcgagt tcgcctccat cttcgcctct ttcggaactc gggtggccgt
     1321 ttacgagatg ctgccgcgcc tgctcccgct ggaggatccc gaggtcggcc gcaccatcga
     1381 acgcgccttc aagaaacggg gcatcgccgt ccagaccggc cggccggccg agcgggccga
     1441 actggaccgg ttccagctgg tgctggtggc catcggccgc cagccggtga ccgccggcct
     1501 cgggctggaa gaggccgggg tagcgctgga cggcaagggg ttcgtccgga ccggggagga
     1561 ccttcagacc agccggccgg ggatttacgc catcggcgac atcgccggcc ggggccagct
     1621 ggcctacctg gccggcgagc agggaatctc ggtggtcgag gggatcctcg gcaaccgccg
     1681 cccggtggcc tgcgaattcc tgccttcctg cgtcttcacc cagccggagg tcggcacctt
     1741 cggagtgacc gaggaacagg ccggccggga gccgggccgc taccggatcg gccgcttccc
     1801 gtttaccgcc ctgggcaagg cgcactgcct gggagagacc gagggcttcg tcaagctggt
     1861 cgccgaaacc gccaccggcc gcatcgccgg aggccagatc gtcggacccg gcgccgccga
     1921 cctggtccac ctcgccagcc tggccgccgc ctcggagggc accgtcgaaa cggtggccga
     1981 ggccatcttc ggccacccga ccctggccga aagcgtcaag gaggcggccg ccgatatcct
     2041 gggcctggcc tcgcacctgc cggccagaaa agccgccggc gcctgaaccg gacccggccg
     2101 gggaaagcca gt
//
LOCUS       DAOQYN010000267         2084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_61706_length_2084_cov_4.450468, whole genome shotgun sequence.
ACCESSION   DAOQYN010000267 DAOQYN010000000
VERSION     DAOQYN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61706_length_2084_cov_4.450468"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..836)
                     /locus_tag="PKN80_08670"
     CDS             complement(144..836)
                     /locus_tag="PKN80_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNQ36119.1"
                     /translation="MRALTLSLVLHLGLGLLLVLAAVWPLRERPQPISAETVFLVANL
                     PVLPAAAPLEEASPETSREAGSVAPETLPVPQPPEPSLAERIQTRLAGIPATSVPVGP
                     KHERTPVAVPAVRPEKSPSGTAAAVPPAGVVIEAGFNDPLYLAMLIRRLTESWRPPRE
                     GLAVVKREAGVSFIINRSGGVEEVLLMRPSGDSRFDRSAVSAVSGAAPFPPLPAAWPR
                     DRLQVSVRFRDD"
     gene            complement(833..1252)
                     /locus_tag="PKN80_08675"
     CDS             complement(833..1252)
                     /locus_tag="PKN80_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNQ36120.1"
                     /translation="MKRLKVAPPLVDQINITPMLDTAMTLLLIFVIISPIIGRGIAVK
                     IPEARGEKLPVTSTVVLTMDRSGRVFLGSKPVNPIFLPVDLQNLKLASPDLKVIIQAD
                     RDVRYQNVASLLDLVRQAGISSVGLATRPPGGSEDRK"
     gene            complement(1249..1848)
                     /locus_tag="PKN80_08680"
     CDS             complement(1249..1848)
                     /locus_tag="PKN80_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNQ36121.1"
                     /translation="MKWLLSFQMTGWFTGGIYVVLILLSVASWALIFQKLSLLRRIRR
                     AEALFKATRPEDLESIQGRLAASSLYLVYREKEQFRAQGKKDRECLEEAIRVSLNEHF
                     QDVGSGMSVLATITSVAPFLGLLGTVWGIMLIFSKLHGQVAGVSEMVAPGVAQALLTT
                     IGGLLVAIPALFFYNYFSMILKHLSNEAENYASRLIYLK"
BASE COUNT          463 a          638 c          628 g          355 t
ORIGIN      
        1 tggagtcctt ttgaaaaaaa ccggtggcca ggcaaccgtt cgggatttta aaaaaataaa
       61 ggttgaaaaa ccagggttgt attcttatta tatcacacca aaagggcccg ccgggaccgc
      121 cgtgcattta cgatagtatc cactcagtcg tcccggaacc gaacactcac ctggagacgg
      181 tcccggggcc aggcggccgg caggggcgga aacggcgccg ctccggagac ggccgatacc
      241 gcggaacggt caaaacggga atcgccggag ggacgcatca gcaggacctc ctccactccc
      301 cccgaccggt tgataataaa agaaactccc gcctcgcgct tgaccaccgc caatccttca
      361 cgcggcggcc gccagctttc ggtaaggcgg cggatcagca tggccagata aagcgggtcg
      421 ttgaacccgg cctcgataac caccccggcc ggcgggacgg cggccgccgt cccggacggg
      481 gacttctccg gccggacggc cggaaccgca accggagtcc tttcatgctt cggcccgacc
      541 ggaaccgagg tcgccggaat cccggccaac cgggtctgga tgcgttcggc caggctcggt
      601 tccgggggct gcggaaccgg gagcgtctcc ggggcaacgg acccggcctc ccgactcgtt
      661 tccggggacg cttcctcgag gggcgccgcc gccggaagga ccggcaggtt ggccaccagg
      721 aagaccgttt cggccgaaat cggctgaggc ctttcccgca gcggccagac ggcggccagc
      781 accaacaaca gtcccaagcc gagatgcagg accagggaga gcgtcagagc tctcatttcc
      841 ggtcttcgga tccccccggc ggccgggtgg ccaggcctac cgaactgatg ccggcctggc
      901 gcaccaggtc aagcagcgag gccacgttct ggtagcgcac gtctcggtcg gcctggatga
      961 tcaccttcag gtccgggctg gccagcttca ggttctgcag gtcaaccggc aaaaaaatcg
     1021 ggttgaccgg tttggaaccc aggaagaccc ggccgctccg atccatggta agcactaccg
     1081 tcgaggtgac cggcagtttt tcgccccggg cctccggtat tttcacggca atgccccggc
     1141 cgatgatcgg agagatgatc acgaagatga gcaagagagt cattgccgtg tcgagcatcg
     1201 gcgtgatgtt gatctgatca accaggggcg gcgcaacttt caggcgtttc atttcagata
     1261 gatcaagcgg ctggcgtaat tctcggcctc attcgaaaga tgcttgagaa tcattgaaaa
     1321 gtagttgtag aagaagaggg ccgggatggc caccaggagc ccgccgatgg tggtcaggag
     1381 cgcctgggcc actccgggcg ccaccatctc cgaaaccccg gccacctgcc cgtgcaattt
     1441 ggagaagatg agcatgatcc cccagacggt gcccagcagg cccaggaatg gcgccacgct
     1501 ggtgatggtg gccaacacgc tcatcccgct gccgacatcc tggaagtgct cattaaggga
     1561 gacccggatg gcctcttcga gacattcccg gtccttcttc ccctgggccc ggaactgctc
     1621 cttctcccgg taaaccaggt aaaggctgga agcggccagc cgaccctgaa tcgactccag
     1681 gtcctcggga cgggtcgcct taaaaagggc ttcggcgcgc cttatccggc gcagcaatga
     1741 gagtttttga aaaatcaggg cccaggaggc caccgagaga agaatcagca ccacgtagat
     1801 ccccccggta aaccagccgg tcatctggaa ggaaagcaac catttcatac tcatgccgca
     1861 ggtctccttt tcggtgaaac cgatgaaaac gcctattgaa ttatgctata acaaaaagtg
     1921 gtaaaggtca aacgttgaaa gtccggatcc atcttcagac taacccgatg agtgccgctt
     1981 gcgtagttgc ctttcgtcct tttccctgaa gccgagggcc cggaacgagg cgagtcgcgg
     2041 agcttctgcc ccggacgcag agtgggaaca aaaaaaaaaa aaaa
//
LOCUS       DAOQYN010000268         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_62560_length_2066_cov_3.953754, whole genome shotgun sequence.
ACCESSION   DAOQYN010000268 DAOQYN010000000
VERSION     DAOQYN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62560_length_2066_cov_3.953754"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PKN80_08685"
     CDS             <1..656
                     /locus_tag="PKN80_08685"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNQ36122.1"
                     /translation="YQPTEEGREKAIKERMKHWQEIRECLARKDRLRSVLIEKRSGLA
                     VEEVNRLSERIGKCLLRHPDFQRARNVALYASFRNEVDTRLILKELLLRPGVRVGFPR
                     TEPGRRQLVFHQVNSPAQLVPGRFGISEPRLKCPVLKASDFDLVLVPGVAFDEAGNRI
                     GFGGGYYDNFLRETRPDAVKIALAYDFQIIKGRLPVLAKDAKVDKIITDERVISAQG"
     gene            1169..1243
                     /locus_tag="PKN80_08690"
     tRNA            1169..1243
                     /locus_tag="PKN80_08690"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1204..1206,aa:Pro,seq:cgg)
     gene            1504..>2066
                     /locus_tag="PKN80_08695"
     CDS             1504..>2066
                     /locus_tag="PKN80_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36123.1"
                     /translation="MATWLAWFLLAVSFGIIFLGSEWFVNSSVRIAEGLRVPKVLVGA
                     TIVSLATTAPELFVSALAAFLGKVGLAAGNAVGSPISNIGFIFGLCVLFTFVPVDQKV
                     IRKQGLMMIGTALMAFAVSFHGMLDRPVAVFFLVLTGLYLWYSVHKARQESRKAPLPE
                     EAIDFRPGRKILLFLLGAALVIGGSRLL"
BASE COUNT          424 a          526 c          624 g          492 t
ORIGIN      
        1 actaccaacc gaccgaagag gggcgggaga aggcgatcaa ggaacggatg aagcactggc
       61 aggagatacg tgaatgcctg gcgcgcaagg accggctgcg cagtgtcctg attgaaaagc
      121 gctccgggct ggccgtcgaa gaggtgaatc ggttgagtga aagaataggc aagtgcctgc
      181 tgcgccatcc tgatttccag cgggccagaa acgtcgccct ttatgcctcc ttccgcaacg
      241 aggtggatac ccgtctcatc ttaaaggaac tgcttctgcg gcccggggtc cgggtcggtt
      301 ttccccggac cgaacccggc cgtcgccagc tggtcttcca ccaggttaac agtccggcgc
      361 agcttgttcc gggtcgtttc gggatttcgg agcccaggtt gaagtgcccg gttttgaaag
      421 cctcggattt cgacctggtc ctggtccccg gggtggcctt tgacgaggcg ggaaaccgga
      481 tcggtttcgg cggcggatac tacgataatt ttctcaggga aacgaggccg gacgcggtaa
      541 agatcgccct cgcctacgac tttcagatta tcaagggccg gctgccggtc ttggcaaaag
      601 acgcaaaagt tgataaaata ataaccgacg aacgggttat cagcgcgcaa ggatgatttt
      661 gatcaggcca acccttcccg ttcgttttgt tgtttttaag ggtggtgcaa acgatcggct
      721 atcagagtat tgaaaatttg ggagtaacag tcatacgcat gtcttatgcg ggtatccaat
      781 ccttaaatga ttttacggat ttttcaggta gtatggatgc tcgtctacga tttccttatg
      841 ccaaggttta cccgccggag ctgtattgta gcgaaggagg atttcggcgg attcgtctga
      901 agataagttc agaaacaata gagggccgtg aagcagagct aagcacgctt cacggcggtg
      961 aagtgtgcgc cgagctctgc gaggcgctct acttcaccgg tgagagaggg ggcggagccc
     1021 cttctcgcgg tcgtgtccgg ggagggggaa tcaggagagg gaagcccctt cccctgaagc
     1081 gagccccgaa actcggcgcg gcttgaagaa ttaacacgga tcggttacac tgcagagtat
     1141 tgaaaattcg agaagtgtta ttgcgtttcg gggcgtagct cagcctggtt agagtgcttg
     1201 gttcgggacc aagaggtcgg aggttcgaat cctctcgccc cgactggggt aacacccggt
     1261 cgaaaccttg aaaacgcaag ttttacaaaa ggattcgaag agagccgtga agtagagtcg
     1321 ctcgacaagc tcgcaacaca cttcacggcc atgaagcgtg cttcgctctg cttcatggcg
     1381 attgttgttt ctgcgatatt tttcagaaga atctaaaaag catttggaat tccaattttc
     1441 aggccgttcg gcgcaacaag tcgaacggct tttttatcca gtaataccta ccggaaaggg
     1501 agaatggcaa cctggttggc ctggttcctg ctggcagtct cctttggcat aattttcctc
     1561 ggttccgagt ggttcgtgaa ctcctcggtc cggattgccg aggggttgcg cgttcccaag
     1621 gtgctggttg gggccaccat cgtcagcctg gccaccaccg ctccggaact tttcgtgtct
     1681 gccctggcgg ccttcctggg caaggttggc ctggccgccg gcaacgcggt tggttcaccc
     1741 atttccaaca tcggatttat cttcggcctc tgcgtcctgt ttacctttgt gccggttgat
     1801 cagaaggtga tccggaaaca gggcttgatg atgatcggca cggccctgat ggctttcgcg
     1861 gtcagttttc acgggatgct tgaccggccg gtagccgtct ttttccttgt tctgaccggt
     1921 ctttacctct ggtactcggt ccacaaggcg cgccaggaga gccgaaaggc gcctttgccg
     1981 gaggaggcga ttgattttcg gccgggccgg aagatactcc ttttcctgct gggagcggcc
     2041 ctggttatcg gcggcagccg gttgct
//
LOCUS       DAOQYN010000269         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_62976_length_2058_cov_3.572142, whole genome shotgun sequence.
ACCESSION   DAOQYN010000269 DAOQYN010000000
VERSION     DAOQYN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62976_length_2058_cov_3.572142"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PKN80_08700"
     CDS             complement(<1..538)
                     /locus_tag="PKN80_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNQ36124.1"
                     /translation="MLTGSILLFRLTGCPAAEPLGEATVRVSAARVRAMPDLHSDILA
                     KVNQGEKIRLLEKKKEWFRVEHAGGSGWVLGELIKIPENVFWRACLAEKTPAALRDYL
                     KAYPAGKFAGAARSRLKKPAAPAPPAEAAVSNTEPAGEAALAGQIRSKATIENCERYL
                     SEYPAGPAAGLARERLDPL"
     gene            complement(589..1476)
                     /locus_tag="PKN80_08705"
     CDS             complement(589..1476)
                     /locus_tag="PKN80_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNQ36125.1"
                     /translation="MAGSGIGSEFELIGRLTRRLPLGVDVLVGPGDDCAVMRYRRNEY
                     LLWTTDLLFEGVHFTRETPPESIGWKALAVSISDIAAMAGRPHYCLVFVGLPAGRTGA
                     YASAIYRGLNRLARRYRVQVVGGDTNAFPRLVIGTTVLGTVEKSYLTRRSGARPGDLI
                     CVSGPLGRGPKSHLSFLPRVSEARRIRRELKATAMIDISDGMLGDFTRISEESRVGGI
                     IRLEEIPVVPGTAPGEALVSGEEFELLFTIPASQAGAARRSGCFVVGEVLPAGRGVRV
                     TANGRPYRPRHPGYDHFRK"
     gene            complement(1502..>2058)
                     /locus_tag="PKN80_08710"
     CDS             complement(1502..>2058)
                     /locus_tag="PKN80_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNQ36126.1"
                     /translation="RTSSGRPPGMPTALDGRRLEPVALIERLNRAGGRHGVGRVDLVE
                     DRTVGLKSREIYEAPAATILITAHRELEKLTLTRSLWELKESLRERYAWMVYQGAWFT
                     EEKEALDAFFNSTQKYVNGQVDLKLYKGSVTAIGRRSPDSLYSEALATYGRGDLFDRT
                     AAKGFIDLAGLPLLLEGRRRLKRR"
BASE COUNT          303 a          688 c          713 g          354 t
ORIGIN      
        1 ggagcggatc gagccgctcc ctggccagtc cggcggccgg cccggccggg tattccgaaa
       61 ggtaccgttc gcagttctcg atggtcgcct tgcttctgat ctgcccggcc agggcggctt
      121 ccccggccgg ttcggtgttg gacacggccg cttccgccgg cggcgccggg gcggccggtt
      181 tcttcaacct cgaccgggcc gcgccggcga actttccggc cgggtaagcc ttcaggtaat
      241 ccctgagtgc ggccggcgtc ttttcagcca ggcaggcccg ccagaagaca ttctccggga
      301 ttttgatcag ttctcccaga acccagccgg agccgccggc gtgctccacc cggaaccatt
      361 ccttcttctt ctcgaggagc cggatttttt cgccctggtt gaccttggcc aggatatccg
      421 aatggagatc gggcatggcc cggacccggg ccgccgagac gcgtaccgtg gcttcgccga
      481 gcggctcggc cgccgggcag ccggtcagcc ggaataaaag gatgcttccg gtcagcacgg
      541 ctgcggtgac cttgagaaat ctggttcgga gggagcggaa acggtccgtc attttcggaa
      601 atggtcgtag ccgggatgcc ggggccggta agggcggccg ttggcggtca cccttacgcc
      661 ccggccggcc ggcagcacct cgcccaccac gaagcatccg gatcggcggg ccgccccggc
      721 ttgggaggca gggatcgtga agagcagttc gaattcctcg ccgctgacca gggcctctcc
      781 aggtgcggtc ccgggcacga cgggtatctc ctccagccgg atgattccgc cgacccggct
      841 ctcttccgaa atccgggtga agtcgcccag cattccgtcg gagatgtcga tcatggcggt
      901 ggctttcagt tcccgcctga tccggcgcgc ctcactgacc ctgggcagga aggagaggtg
      961 gcttttcggg ccgcggccca gcgggcccga gacgcagatc aggtcgcccg gccgggcgcc
     1021 cgagcggcgg gtcaggtagg atttttcgac cgtgcccagc accgtggtgc cgatgaccag
     1081 ccgcgggaag gcgttggtgt cgccgccgac cacctgcacc cggtagcgcc gggccagccg
     1141 gttgaggccg cggtagatgg ccgaggcgta ggccccggtc cggccggccg gcagccccac
     1201 gaagacgagg cagtagtgcg gccggccggc catggcggcg atgtcggata tcgagacggc
     1261 cagggccttc cagccgatgc tttccggcgg ggtttcccgg gtgaagtgga cgccttcgaa
     1321 gagaagatcg gtggtccaga ggagatactc gttgcggcgg tagcgcatca cggcgcagtc
     1381 gtcgccgggg ccgaccagga catccactcc cagcggcagc cggcgggtca accggccgat
     1441 gagttcgaat tcgcttccga tcccggaccc ggccatgcct ccctcctggc gagtgttctg
     1501 ctcagcgccg cttcagccgg cgccgcccct cgaggagcag gggcaggccg gccaggtcga
     1561 tgaagccctt ggcggccgtg cggtcaaaga ggtcgccccg gccgtaggtg gccagcgcct
     1621 cgctgtagag tgagtcgggc gaccggcgcc cgatggccgt aacgctcccc ttgtagagct
     1681 tgaggtccac ctgcccgttg acatacttct gggtggagtt gaagaaggcg tccagcgcct
     1741 ccttctcctc ggtgaaccag gcgccctggt aaaccatcca ggcgtagcgc tcccggagcg
     1801 attccttcaa ctcccagagt gaacgggtca gggtgagttt ttcgagttcc cggtgggcgg
     1861 ttatcagaat ggtcgccgcg ggcgcttcgt aaatttcgcg gctcttgagt ccaaccgtcc
     1921 ggtcttccac caggtcgacg cgtcccacgc cgtggcggcc gccagcccgg ttgagccgtt
     1981 cgatcagggc caccggttcc agccgccgtc cgtccagcgc ggtcggcatg ccaggaggcc
     2041 tcccggagga ggtccgcg
//
LOCUS       DAOQYN010000270         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_63728_length_2043_cov_3.069920, whole genome shotgun sequence.
ACCESSION   DAOQYN010000270 DAOQYN010000000
VERSION     DAOQYN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63728_length_2043_cov_3.069920"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PKN80_08715"
     CDS             complement(<1..765)
                     /locus_tag="PKN80_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNQ36127.1"
                     /translation="MTTRKSRKLGSAADLDRIRNEFKAVLEARQGKRSDSGLSQIMVC
                     TGASCIACGALECKAALQEAVKKYGLATRVGVVETGCLGPCCLGPVAVVSPDTVFYQK
                     MTPADATEIVEKHLLNHKPVERLLLRKVGTEEILAHLSDIDFFTRQKKIVLHNCGWID
                     PVRIEEYFGADGYQALARVISEQAPDRVIEALKKSGLRGRGGAGFPTWLKWNLARQAR
                     GEKKFVICNADEGDPGAFMDRSILEGDPHAVIEAMVI"
     gene            complement(783..1298)
                     /locus_tag="PKN80_08720"
     CDS             complement(783..1298)
                     /locus_tag="PKN80_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNQ36128.1"
                     /translation="MTDKKKSGGCEVQFDEAAGLEQLRSAIGLYRRKPGALIPILQAA
                     QEIFGYLPESALRLISGELKKSYSEVAGVVSFYSFFSTTPRGKHLVRVCLGTACYVRG
                     GKEVLDALRQKLGIEVGETTADRNFSLEVARCFGACGLAPVIMIDEDVHQRVKPRKVN
                     EILDRYRTRQS"
     gene            complement(1668..>2043)
                     /locus_tag="PKN80_08725"
     CDS             complement(1668..>2043)
                     /locus_tag="PKN80_08725"
                     /inference="COORDINATES: protein motif:HMM:NF018651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNQ36129.1"
                     /translation="HMMSFGPYLPHHQTPLAESPNGPEGLTLKVMALARLLVEKANMP
                     VTTALATLDPADGLTSGLKAGANVIMVNFTPEPRRFNYTIYDHKLRVDFEQVRNAAAR
                     AGRTVSLERGDAPGWEGTGPLA"
BASE COUNT          365 a          650 c          630 g          398 t
ORIGIN      
        1 gataaccatc gcctcgatca ccgcgtgggg atctccctcc aggatgctgc ggtccatgaa
       61 ggcgcccggg tcaccctcgt cggcgttgca gatgacgaat ttcttttcgc cccgcgcctg
      121 gcgcgccagg ttccacttca gccaggtcgg gaaaccggca ccgccccgac cgcgcaaacc
      181 ggacttcttg agcgcctcga tcacccggtc cggcgcctgc tcggaaatga cccgggccag
      241 ggcctggtac ccgtcggccc cgaaatactc ctcgatgcgc accgggtcga tccagccgca
      301 gttgtgcagg acgatcttct tctggcgggt gaaaaaatcg atatcggaga ggtgggccag
      361 gatttcttcg gtgccgacct tccggagcag cagccgctcc accggcttgt ggttcagcag
      421 gtgcttctcc acgatctcgg tggcatcggc cggcgtcatc ttctggtaga agaccgtgtc
      481 cgggctgacc acggccaccg gccccaggca gcaggggcca aggcagccgg tctccaccac
      541 cccgacccgg gtggccaggc catacttctt gacggcctcc tgcagggcgg ccttgcactc
      601 cagggcgccg caggcgatac agctggcgcc ggtgcagacc atgatctggg acagcccgga
      661 atcgctccgc ttgccctggc gcgcctccag caccgccttg aattcgttcc ggatccggtc
      721 caggtcggcc gcgctgccga gtttccgact cttccgggtg gtcattttct tctcctccat
      781 gatcaggatt gcctggttcg gtaacgatcg agtatctcgt tcaccttgcg cggcttgacc
      841 cgctggtgga cgtcttcatc gatcatgatg accggcgcca ggccgcaggc cccgaaacag
      901 cgggccacct ccagggaaaa attgcggtcg gcggtcgtct cgcccacctc gatgccgagt
      961 ttctgccgga gggcatccag gacttcctta ccgccccgga cataacaggc ggtccccagg
     1021 cagacccgga ccaggtgctt gccgcgcggc gtggtggaaa agaacgagta gaagctcacc
     1081 acgcccgcca cctcgctgta ggatttcttc aactcgccgc tgatcaaccg cagggccgat
     1141 tccggcaggt agccgaagat ctcctgggcc gcctggagaa tcggtatcag ggcgcccggt
     1201 ttgcgacggt aaagtccgat ggcgctgcgc aactgctcca gtccggctgc ctcgtcaaac
     1261 tggacctcgc agccgccgga tttcttcttg tcggtcattc cgttcctccg gtcttgaccc
     1321 cgggctgtcg tccgttcgtg cctttgagta cgggaagcga aaaagccggc cccgggcttg
     1381 aaaagccttt atttatgaat atttatggta aatcggcccc tgcgaagaca ggtcaaaacg
     1441 gcttaaagag gctgtttcaa tcgttattaa aagcacaaac cttattcacc tcattttatc
     1501 ccacgctggt tggcttgtca atcggcggcg atgaagaaaa aagaggtgga tttatggttg
     1561 gaatcggccg ggaaacccgt ccggaaaccg ggaacaaaac cgcgccatta acctggctgc
     1621 ggaaaaaccg gccgaagggg agcggttttc aagccggcgg gctgatgcta tgccagggga
     1681 ccggtgcctt cccagccggg agcgtcgccc cgttcgagcg aaacagtccg cccggcccgg
     1741 gcggcggcgt tccggacctg ttcgaaatcg acccggagct tgtggtcgta aatggtgtag
     1801 ttgaaacggc gcggttccgg ggtgaagttg accatgatca cgttggcgcc ggccttcaga
     1861 ccgctggtca ggccgtcggc cggatccagg gtggccaggg cggtggtaac cggcatgttg
     1921 gccttttcaa ccagcaggcg ggccagcgcc atcaccttca gggtcaaccc ttccggaccg
     1981 ttcggcgatt ccgccagcgg cgtctggtgg tgcggcaggt agggtccgaa ggacatcatg
     2041 tgg
//
LOCUS       DAOQYN010000271         2008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_65556_length_2008_cov_3.112647, whole genome shotgun sequence.
ACCESSION   DAOQYN010000271 DAOQYN010000000
VERSION     DAOQYN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65556_length_2008_cov_3.112647"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..907
                     /locus_tag="PKN80_08730"
     CDS             71..907
                     /locus_tag="PKN80_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNQ36130.1"
                     /translation="MVGTSILILGGLSLAFAVLLAFAARFFTVSEDPLVAKIEELLPG
                     SNCGGCGAAGCRALAELLAARKVDPGICVASNAETNAKIAALTGQSVNLVEKRVAVLR
                     CQGGRSRVKERFRYTGIPTCTVANLTAGGPKECLYGCLGYGDCLRACPFGAISLGEEG
                     LPVIDEAKCVACGRCVAACPRRLVTLISRRQWTYVACSSRNKGKTVKDACPVGCIACG
                     LCAKVTKSGAIVIEDNLPRMNYEKGQDFEEAVKRCPMHCFVVRFTPRTSSDAGSEPAA
                     KA"
     gene            complement(855..2006)
                     /locus_tag="PKN80_08735"
     CDS             complement(855..2006)
                     /locus_tag="PKN80_08735"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNQ36131.1"
                     /translation="MIADGLGGLPDSATGRTELETARTPVLDSLARNGGAAGLSVAIG
                     YGITPGSGPSHLALFGYDPVENLIGRGVLEASGIDFPLEPEDLAVRGNFATLRDGLIT
                     DRRAGRIPTEENQRIVERLGRELKEIEGVQVILRTVKEHRFVVVFRGPGLEAGLTEND
                     PEREGQAPLEIKPLHPAAARSARIAGLFVDRVVELLASEKSANYPLLRGFARKPALPS
                     FRDRYRLKSAAIATYPMYRGLARLVGMDVLATGDTLASEAETLKKHYRDYDFFYLHVK
                     KTDSYGEDGNFAAKVRVIEELDALVGEILGMKPAVLAVTADHSTPARLAGHSWHPNPF
                     LLASEWALPDQLAAFSERECRRGSFGVFPATEIMPLLLAHSRRLKKFGA"
BASE COUNT          353 a          672 c          642 g          341 t
ORIGIN      
        1 ctgatcctgg ccggcctgat cgccctttcc ttcatgggct tcgcgggact actctaagag
       61 gaaaacatcc atggtcggaa cttccatcct gatactgggc gggctgtcgc tggccttcgc
      121 ggtcctgctg gccttcgccg cccgattctt tacggtctcc gaagacccgc tggtggccaa
      181 aatcgaggaa ctgctgccag gttcaaactg cgggggatgc ggggcggccg gctgccgggc
      241 cctggcggaa ctcctggccg cccgcaaggt ggacccgggc atctgcgtgg ccagcaatgc
      301 cgagacgaat gccaaaatag ccgcgctgac cggccaatcg gtcaacctgg tcgaaaagcg
      361 ggtggccgta ctccgctgcc agggcggccg cagccgggtg aaggaacgtt tccggtacac
      421 cggcatcccg acctgcacgg tggccaacct caccgccggc ggtcccaagg aatgccttta
      481 cggctgcctg ggttacgggg attgcctgcg ggcctgcccg ttcggggcca tcagcctggg
      541 cgaggaaggt cttccggtga tcgacgaagc gaagtgcgtc gcctgcggac gctgcgtggc
      601 cgcctgcccg cggcgcctcg tcaccctgat atcgcgccgg caatggacct acgtggcctg
      661 ctcttcacgg aacaagggca aaacggtcaa ggatgcctgc ccggtgggtt gtattgcctg
      721 cggactctgc gccaaggtga ccaagtccgg ggccatcgtg atcgaagaca acctgccccg
      781 gatgaattac gagaagggac aggatttcga ggaagcggta aaacgctgcc cgatgcactg
      841 cttcgtggtc cgcttcacgc cccgaacttc ttcagacgcc gggagtgagc cagcagcaaa
      901 ggcataatct cggtggccgg gaagaccccg aagcttcccc gccggcactc cctttcggaa
      961 aaggcggcca gctggtcggg caaggcccac tccgaagcca gcaggaacgg attgggatgc
     1021 catgaatggc cggccagccg ggccggagtg gagtggtcgg cggtaacggc cagcaccgcc
     1081 ggcttcatac ccagtatctc acccaccagc gcgtccagtt cctcgatgac ccttaccttg
     1141 gcggcgaaat tgccgtcctc tccgtaggaa tcggtcttct tgacgtgcag gtagaagaaa
     1201 tcgtaatccc ggtagtgctt cttaagggtc tccgcctccg aagccagggt gtcgccggtg
     1261 gccaggacat ccattcccac cagccgcgcc agtccccggt acatcgggta ggtggcgatg
     1321 gccgccgact tcagccggta gcggtcccgg aaggagggca gggcgggctt gcgggcgaaa
     1381 ccgcgcagca ggggatagtt ggccgatttc tccgaggcca gcaattcaac cacccggtcc
     1441 acgaaaaggc ccgcgatcct ggccgaacgg gccgccgccg gatgcagggg cttgatctcc
     1501 agcggggcct ggccttcccg ttccggatca ttctcggtca ggccggcttc caacccgggg
     1561 ccccggaaga caaccacgaa acggtgctcc ttgaccgtcc gcaggataac ctggacaccc
     1621 tcgatctcct tcaattcgcg ccccagccgt tccacgatcc gctggttttc ctcggttgga
     1681 atccggcccg cccgccggtc ggtgatcagg ccgtcccgca gggtggcgaa attgccgcgc
     1741 accgccaggt cctccggttc caggggaaaa tcgatgccgc tggcctccag caccccccgt
     1801 ccgatcaggt tttccaccgg gtcatagccg aagagggcca ggtggctggg tccgctgccc
     1861 ggggtgatgc cgtaaccgat cgcgaccgaa agaccggccg cccctccatt ccgggccagg
     1921 ctatccagga ccggcgtccg ggccgtctcc agctcggtgc ggccggtggc cgaatccggt
     1981 aagccgccca gaccgtcggc aatcacca
//
LOCUS       DAOQYN010000272         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_66239_length_1995_cov_2.853093, whole genome shotgun sequence.
ACCESSION   DAOQYN010000272 DAOQYN010000000
VERSION     DAOQYN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66239_length_1995_cov_2.853093"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PKN80_08740"
     CDS             complement(<1..460)
                     /locus_tag="PKN80_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36132.1"
                     /translation="MNRRNKLNFWLGIILAAVTLAFAAGCGGGGGGSSVRLDTNLLGL
                     ASKGPIEGGTVSIYALDAAGNQGALLGTAITDDDGSYSIDIGGYQGNVLVQVTGGTYL
                     DEATNLPVAFAGTLRAALTNVTGTVSVAVTPLTELAVLQAGLPLTGDRINR"
     gene            692..>1995
                     /locus_tag="PKN80_08745"
     CDS             692..>1995
                     /locus_tag="PKN80_08745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNQ36133.1"
                     /translation="MKKVLLTTVTKPFPPLDGVNSFDLYASRLTKYQDIFTVSSYYPA
                     LPLFLIAQNLQAESVVLEHPTVAQFERELARGYDYIGIHFAASFFFDKVLAMVRTIRR
                     LSPGTKIVLGGYGVTCLTQEFAQEKELAGLVDYVCQEEGVCFMRKVMGEPVDRPIKQV
                     FPAARVNFMGQRFLFDNMLAALGCRAGCEFCATSAFFKYRKIRVATPAELWTHLKRNI
                     REKDVAFNWIYDEDFFADPDYVREFAALIRKDPDFDLKKVCWGGYGSVRTLSRFTAEE
                     LAGMGVTSLWIGVESKFSELPKRQGRDIKETFDSFRKAGIQIVGSFIIGWDFHTPENV
                     REDIDFLTSLNPTFCQVSSLMPCPGTKFWDKVVQEGRLKADEFRWDHFHLYSSIHKHR
                     NLDEREIPGLMKLTHRSLLEHNGPSVYRLFEVHLKGYASFKD"
BASE COUNT          377 a          610 c          602 g          406 t
ORIGIN      
        1 cccggttgat ccggtccccg gtcaggggca ggccggcctg caggacggcc agctcggtca
       61 gcggggtgac cgccaccgag acggtcccgg tgacattggt cagggcggcc cggagcgtgc
      121 cggcgaaggc gaccggcagg ttggtggcct catccaggta ggtaccgccg gtcacctgga
      181 ccagcacgtt gccctggtag ccgccgatat cgatggagta gctgccgtca tcgtcggtga
      241 tcgcggtgcc gagcagtgcc ccctggttcc ccgccgcgtc cagggcgtat atggagaccg
      301 tacccccttc gatcggcccc ttggaggcca ggccgagcag gttggtgtcg aggcggaccg
      361 agctgccgcc gccaccgccg ccgcagccgg cggcgaaggc cagcgttacc gccgccagaa
      421 tgattccgag ccagaaatta agcttattcc gtctgttcat gctgtgcctc cccgggtctg
      481 gttttaaaaa cagtattgta gccgtccggg caaccttttt tcgccccggc cggccggttg
      541 aaatctattg taaacggagg ggggtttcct gtcaaggttg ggaagcgggt ttatttgacc
      601 ttttatagca taagagggta aaatttctta ttaacaattt attaaagaaa agttaaagac
      661 cgggggattt ttttataatg aggccgcctc aatgaaaaaa gttttgttga caaccgtaac
      721 gaaaccattt ccgcccctgg atggggtgaa cagcttcgat ctttacgcga gccgcctgac
      781 caagtaccag gatatcttca cggtcagcag ttactacccg gccctgccgc tcttcctgat
      841 cgcccagaat ctccaggcgg agtcggtcgt cctggagcac ccgaccgtcg cccagttcga
      901 acgggaactg gcccgcggct atgactacat cggcatccac ttcgcggcct ccttcttctt
      961 cgacaaggtc ctggcgatgg tccggacgat ccgccgtctt tcgcccggga ccaagatcgt
     1021 gctgggcggg tacggcgtca cctgcctgac ccaggagttc gcccaggaga aggagctggc
     1081 cggcctggtt gattatgtct gccaggagga gggggtctgc ttcatgcgga aggtgatggg
     1141 tgaaccggtt gaccggccga tcaagcaggt cttcccggcc gcccgcgtca atttcatggg
     1201 gcagcggttc ctcttcgaca acatgctggc cgccctgggc tgccgggccg gctgtgaatt
     1261 ctgcgccacc agcgccttct tcaagtaccg caagatccgg gtggccaccc cggccgaact
     1321 ctggacgcac ctgaagcgga acatccggga gaaggatgtc gccttcaact ggatctacga
     1381 cgaggatttc ttcgctgatc ccgattacgt ccgggagttc gcggccctga tcaggaagga
     1441 cccggacttc gacctcaaga aggtctgctg gggcggctac gggagcgtgc ggaccctctc
     1501 ccgcttcacc gccgaggaac tggccgggat gggcgtcacc tccctctgga tcggggtgga
     1561 gtcaaagttc agcgaactgc ccaagcgcca gggtcgggac atcaaggaga ccttcgattc
     1621 cttccggaag gccggcatcc agatcgtcgg ttccttcatc atcggctggg atttccacac
     1681 cccggagaat gtgagggagg atatcgattt cctgacttcg ctcaacccca ccttctgcca
     1741 ggtctcgagc ctgatgccct gcccgggcac caagttctgg gacaaggtcg tccaagaggg
     1801 tcgcctcaag gccgacgagt tccgctggga ccatttccac ctttactcat ccatccacaa
     1861 gcatcgtaac cttgacgaac gggaaatacc cggcctgatg aagctgaccc accggagcct
     1921 gctcgagcat aacgggccct ccgtctaccg gctttttgaa gtccacctga aggggtacgc
     1981 ttcgttcaag gacag
//
LOCUS       DAOQYN010000273         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_66287_length_1994_cov_4.089221, whole genome shotgun sequence.
ACCESSION   DAOQYN010000273 DAOQYN010000000
VERSION     DAOQYN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66287_length_1994_cov_4.089221"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKN80_08750"
     CDS             <1..261
                     /locus_tag="PKN80_08750"
                     /inference="COORDINATES: protein motif:HMM:NF040137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HNQ36134.1"
                     /translation="IERISADTQELLTMLKHPRRFFWLNFMMGVVRGLGIFVGATILG
                     TFVVAFFLILLRWMERMPLLGDLVRFIVNLIRDLIRQPVSVP"
     gene            273..1343
                     /locus_tag="PKN80_08755"
     CDS             273..1343
                     /locus_tag="PKN80_08755"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNQ36135.1"
                     /translation="MWALVYLAIFLVSCLLSVLLVPFVRRVALAQGLVDHPGPRKVHQ
                     NPTPLLGGAAILVALLGTVLFGYLAFIPFGHLLPVALHRHLAGVVSVLPRLSLILFGG
                     VLIFLFGLVDDLVGIRARWKLLGQAAIALLLFASGLRISLFLPGTAVPLLLTVGWLLF
                     VTNSFNLLDNMDGLASGVAFIAAALFFLYAQVTGALFVSSLLAAFAGAVLGFLVYNRP
                     PATIFLGEAGSTLLGYFLAVTGVTLTYYRQDSPSYLSVLAPVFILAIPIFDTLSVIAI
                     RRREGRSIFTAGKDHLSHRLVRFGFSSREAVSIIYLLSLGLGLPALVLSHLSFAGSLI
                     LLLQGCIFLAVLSFLESRGNAS"
     gene            1365..>1994
                     /gene="pyrF"
                     /locus_tag="PKN80_08760"
     CDS             1365..>1994
                     /gene="pyrF"
                     /locus_tag="PKN80_08760"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNQ36136.1"
                     /translation="MTEQERIIVALDYQRLDEALSLVDRLAGAASFFKVGLELFAGAG
                     PEAVRELKDRGQRVFLDLKFFDIPNTSARASRQAVRLGVDLFNLHLLAGVEVLQAAAA
                     AAREEAAKLGVAPPRLLGVTILTSSQGEEWRELGLAAPVSELVLRLAERARRSGLDGV
                     VASPREAAGLRARFGADFLLVCPGIRPVGAAAADQKRVLSAREARQAGAD"
BASE COUNT          232 a          689 c          631 g          442 t
ORIGIN      
        1 atcgagcgca tctccgccga cacccaggaa ctcctcacca tgctcaagca tccccgccgc
       61 ttcttctggc tgaatttcat gatgggggtg gtgcgcggtc tggggatttt tgtcggggcc
      121 accatcctgg gcaccttcgt ggtggctttt ttcctgatcc tgcttcggtg gatggaacgg
      181 atgcccctgc tgggggacct ggtccgtttt atcgtcaacc ttatccgcga cctgatccgg
      241 cagccggtct cggtgcctta aggaaaaacc cgatgtgggc cctggtatac ctggccatct
      301 ttctcgtcag ctgtctcctg tcggtcctgc tggtgccttt cgtgcgccgg gtcgccctgg
      361 cccaggggct ggttgaccac cccggtccgc gcaaggtcca ccagaacccc acccccctcc
      421 tgggcggggc ggccatcctg gtggccctgc tgggcaccgt cctcttcggc tacctggcct
      481 tcattccgtt cgggcacctg ctgccggttg cgctccaccg ccacctggcc ggtgtcgtga
      541 gcgtgctgcc ccgtctctcc ctgattctct tcgggggcgt gctcatcttt cttttcgggc
      601 tggtggacga cctggtgggg atccgggccc gctggaaact gctcggccag gcggccatcg
      661 ccctgctgct tttcgcctcc gggctccgca tcagcctctt cctgcccggg acggccgtgc
      721 cgctgctgct taccgtcggc tggctccttt ttgtcaccaa cagtttcaac ctcctggaca
      781 acatggacgg cctggcctcg ggggtggcct tcatcgccgc ggccctcttt ttcctctacg
      841 cccaggttac cggggcgctt ttcgtctcct cgctgctggc cgccttcgcc ggggcggtgc
      901 tcggattcct ggtttacaac cggccgccgg ccaccatctt cctgggtgaa gccggctcga
      961 ccctgctggg ctatttcctg gccgtgaccg gggtgaccct gacctattac cggcaggatt
     1021 cgccctccta cctgtcggtc ctggccccgg tcttcatcct ggccataccg attttcgata
     1081 cgctttcggt gatcgccatc cggcgtcgcg aggggcgttc gatcttcacc gccggcaagg
     1141 atcatctttc ccaccggctg gtccgattcg gcttctcctc ccgggaggcg gtcagcatca
     1201 tttacctgct ctcgctcggg ctgggactcc cggcactggt cctgagccac ctgtccttcg
     1261 cgggtagcct tatcctgttg ctgcaggggt gcatcttcct ggcggtgctc tcgttcctgg
     1321 agagccgcgg caacgcctct tgaaactttc tcccgggaat cggaatgact gaacaggaac
     1381 ggattatcgt cgccctggat taccaacggc ttgacgaagc gctcagcctg gttgaccggc
     1441 tggccggggc ggcctctttc ttcaaggtgg ggctggaact tttcgccggg gccggaccgg
     1501 aagcggtgcg tgaactgaaa gaccgcggcc agcgggtctt cctggacctg aagttcttcg
     1561 acatccccaa cacttccgcc cgggccagcc ggcaggcggt ccggctgggg gtcgatcttt
     1621 tcaacctcca cctgctggcc ggggtcgagg tgctccaggc ggccgccgcg gcggcccggg
     1681 aggaggcggc gaaactgggc gtcgccccgc cccgtctgct cggggttacc attctcacca
     1741 gcagccaggg cgaggagtgg cgggagctgg gattggccgc gccggtttcc gaactggttc
     1801 tgcggctggc ggagcgggcg cgccggtccg gcctggacgg cgtggtggcc tcaccccgcg
     1861 aagcggccgg tttgcgtgcc cggttcgggg ccgatttcct gctggtctgc ccgggcatcc
     1921 gtccggtcgg cgccgcggcc gccgatcaga aacgcgtcct ttcagcccgg gaggcccgtc
     1981 aggccggcgc cgat
//
LOCUS       DAOQYN010000274         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_66513_length_1990_cov_5.476486, whole genome shotgun sequence.
ACCESSION   DAOQYN010000274 DAOQYN010000000
VERSION     DAOQYN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66513_length_1990_cov_5.476486"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1633
                     /locus_tag="PKN80_08765"
     CDS             <1..1633
                     /locus_tag="PKN80_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36137.1"
                     /translation="GLYLLLEEPRYFEADDPFWKEHPDLRGASGGWGVPDRRPIYYIC
                     TSTEAGRAYLYENALRLHRFVPELAGSILITASEYPTHCYSHVITNPGGHIFASDQEH
                     EGIDCPRCGRRPPEEVVAEIINTYREAVRQSGSAAAVIAWNWSWVMYSPNPQPELIDR
                     LDPEVQVLADFEIGGRTNIYGKERRVEEYSLIFVGPGDRYASVTGYARKQGHRHLAKL
                     QVGTTHELATAANLPLLGNLFRKLDYLRRDGSGGFLASWNFGNRFTINSAAVGLAVAR
                     PDLDTEADFLAELCRSYLGRQETDRFVHAVGIMEAAFREFPIANRLLHIGPLNYALAA
                     PLDGSPLQGKPLSASWLALERGDNWEECLGPYTLDEVITGLGRLAVKLEEGLRELEKV
                     LFAGVDPWWSGLTDYRGEAVIPRHDDRISDQERVTRLIRLLPGECRRRLPGLENIHGY
                     RCLQEWTNGWAVLCFLESAGRLFDNYRARKAAGPGYPEYLDRLRQEELRTVRRALPLF
                     RLDERLGLHLECQEYLVSRSLLEAKEKSLSAEVRA"
     gene            1704..>1990
                     /locus_tag="PKN80_08770"
     CDS             1704..>1990
                     /locus_tag="PKN80_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36138.1"
                     /translation="MKGVITGLLFSACLVCFLSAPVPARAAGRYFEAEEIGSLLRYPY
                     DNEAAAGWYAREANIRSFGAPGRGYCAAIHANASAGGRIISRPLAEPVPAG"
BASE COUNT          311 a          673 c          653 g          353 t
ORIGIN      
        1 cggcctctac ctgctcctgg aggagccccg ctacttcgag gccgacgatc ccttctggaa
       61 ggagcatccc gatttgcggg gcgcttccgg cggctggggc gttccggacc ggcggccgat
      121 ttattacatc tgcaccagca ccgaggccgg ccgcgcctat ctctacgaga acgcgctccg
      181 gctgcaccgg ttcgttccgg agctggccgg cagcatcctg atcaccgcct ccgagtaccc
      241 cacccattgc tattcgcacg tcatcaccaa tccgggcggc cacatcttcg cctccgacca
      301 ggagcacgaa gggattgatt gcccgcgctg cggccggcgg ccgccggagg aggtggtggc
      361 cgagatcatc aatacctacc gggaggcggt ccgccagtcc ggttcggccg cggcggtgat
      421 cgcctggaac tggtcctggg tcatgtattc tccaaacccc cagccggaat tgatcgaccg
      481 gctcgacccg gaggtccagg tcctggccga cttcgagatc ggcggccgga cgaatatcta
      541 cgggaaggag cgccgggttg aagagtactc cctgatcttc gtcggaccgg gcgaccgcta
      601 cgcctcggtt accggctacg cccggaaaca ggggcaccgc cacctggcca agctgcaggt
      661 gggtaccacc cacgaactgg ccaccgcggc caacttgccc ctgctgggca acctcttccg
      721 aaaactcgat tacctgcgcc gggacggcag cggcggtttc ctggccagct ggaacttcgg
      781 caaccgattt accatcaaca gcgccgcggt gggcctggcg gtcgcgcggc ccgacctgga
      841 taccgaggcg gacttcctgg ccgaactctg ccgctcttat ctgggccgcc aggagaccga
      901 ccggtttgtt cacgcggtcg ggatcatgga ggccgccttc cgggaattcc cgatcgccaa
      961 ccgccttctt catatcggtc cgctcaacta cgccctggcc gcgccgctgg acgggagccc
     1021 gctgcaaggg aaaccgcttt cggccagctg gctggccctg gaacggggcg acaactggga
     1081 ggagtgcctg ggcccctata ccctggacga ggtcattacc ggcctgggcc ggctggcggt
     1141 caagcttgag gaggggctgc gggaactgga gaaggtcctc tttgccggcg tcgatccctg
     1201 gtggtcggga ctgaccgatt atcggggcga ggcggtgata ccgcgccacg acgatcggat
     1261 ctccgaccag gagagggtaa cgcgcctgat ccggctcctg ccgggcgagt gccgccgccg
     1321 gttgcccggg ctggagaata ttcacggtta ccgttgcctg caggaatgga ccaacggctg
     1381 ggcggtcctc tgcttcctgg agagcgccgg ccgccttttc gacaattacc gggcccggaa
     1441 ggcggccggt cccgggtacc cggagtacct ggaccggctc cggcaggaag agttgagaac
     1501 ggtgcggcgg gccctgccgc tcttccgcct ggacgaacgg ctcggcctgc acctggaatg
     1561 ccaggagtac ctggtgagcc gctcgctcct ggaggccaag gagaagtccc tgtcggccga
     1621 agtcagggct tgaaagcccg gtcctttttt tgagaatcag ccccgttttt ctgatcacct
     1681 ttcgcccggt tggaggcaaa tcgatgaagg gagtcatcac cggccttctt ttctccgctt
     1741 gtctggtttg ttttctgtcc gctccggttc cggcccgggc ggccggccgc tacttcgaag
     1801 ccgaggagat cggctccctg ctccgctacc cctacgacaa cgaggcggcc gccggctggt
     1861 atgcccgcga ggccaacatc cgttccttcg gcgcgccggg gcgcggctac tgcgcggcca
     1921 ttcacgccaa tgcgtcggcc ggcggccgga tcatcagccg gccgctggcg gagccggtcc
     1981 cggccggcaa
//
LOCUS       DAOQYN010000275         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_66522_length_1990_cov_3.606718, whole genome shotgun sequence.
ACCESSION   DAOQYN010000275 DAOQYN010000000
VERSION     DAOQYN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66522_length_1990_cov_3.606718"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1990
                     /locus_tag="PKN80_08775"
     CDS             <1..>1990
                     /locus_tag="PKN80_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNQ36139.1"
                     /translation="GGCFQNRPAAELVPGDLIELEAGDHVPADGRIVYHTNNFSVLEA
                     SLTGESTPVLKTSLALEEPEIPLADRANQVYLGTSVSSGKARALVVATGMSTELGRIA
                     GLIQTIGHETTPLQKKLEEFGRFIVYICLALVVMVFLLEWWRGGKLLEIFLTAVSLAV
                     AAIPEGLPAVVTIALAMGVQRMVRRHVLIRKLPAVETLGCTTVICSDKTGTLTRNEMT
                     VQVVYAAGRSLQVTGTGYEPEGEFRLQSDSVDPAGQPTLRQVLETGVLCNSAVLSRTG
                     DRAGITGDPTEGALLVCAAKAGILKEPLEAARPLVEEIPFDSERKLMSMIRRVDGELA
                     AYVKGAPDLLLLNCNRIDDGRSVRELTAADREEIQRVNLGLADQAMRVLAFAWRPLET
                     IPGEYSAGTIERDLVFLGLMAMIDPLREEARRAIARCHTAGIRTVMITGDHRNTAVAI
                     ARQLGIFGPDSLALSGEEVDALDDQALFEKVDRIPVYARVSPEHKLRIVRAWRRRGEV
                     VAMTGDGVNDAPAVKEASIGIAMGIAGTDVTKEVADMVITDDNFASIEAAVEEGRGIY
                     DNIQKFIHYLLSCNAGEILVMFAASLIGLPVPLLPIQILWVNLATDGFPALALGVDPF
                     DPDVMDRPPRPANASVVTRARIQALLLQGSFIAFCSLMA"
BASE COUNT          325 a          667 c          666 g          332 t
ORIGIN      
        1 gggcggctgc ttccagaacc ggccggccgc cgaactggtg ccgggagacc tcatcgagct
       61 ggaggccggc gatcacgtgc cggccgacgg ccgcatcgtt taccacacca acaacttcag
      121 cgtcctggaa gccagcctga ccggagagtc caccccggtg ctgaagacga gcctggccct
      181 cgaggagccc gaaattccgc tggccgaccg ggccaaccag gtttacctgg gcacctcggt
      241 cagcagcggc aaggcccggg ccctggtggt ggccaccggc atgtcgaccg aactgggccg
      301 gatcgccggg ctcatccaga ccattggcca tgagacaact cctcttcaga agaagctgga
      361 ggagttcggc cgcttcatcg tctatatctg cctggcgctg gtggtgatgg tcttcctgct
      421 cgaatggtgg cggggcggga agctgctgga aatctttctg accgcggtca gcctggcggt
      481 ggcggccatc ccggaaggac tgccggccgt ggtgaccatc gccctggcca tgggggtcca
      541 gcgcatggtg cggcgccatg tcctgatccg gaagctgccg gcggtggaga ccctcggctg
      601 taccacggtc atctgctccg acaagaccgg gaccctgacc aggaacgaga tgaccgtcca
      661 ggtcgtctat gccgccggac gcagcctgca ggtgaccggg accggctacg agccggaggg
      721 agagttccgg ctgcaatccg attcggttga cccggccggc cagccgacac tccgccaggt
      781 cctggagacc ggggtcctct gcaacagcgc cgttctttca cggaccggcg accgggcggg
      841 tatcaccggc gatccgaccg agggggccct gctggtctgc gccgccaagg ccggtatctt
      901 gaaagaaccg ctcgaagcgg cgcggcccct ggtcgaggag atcccgttcg actccgaacg
      961 gaagctgatg agcatgatcc ggcgggtgga cggcgaactg gccgcctatg tcaagggggc
     1021 gcccgacctg cttctcctca actgcaatcg gattgatgac ggccggagtg tgcgcgagct
     1081 taccgcggcc gaccgggagg agatccagcg ggtcaacctg ggcctggccg accaggcgat
     1141 gcgcgtcctg gcctttgcct ggcggccgct ggagacgata cccggggagt attcggcggg
     1201 tacgatcgaa cgcgacctgg tcttcctggg cctgatggcc atgatcgacc cgctccggga
     1261 agaggcgcgc cgggcgatcg cccgctgcca taccgccggc atcaggaccg tcatgattac
     1321 cggcgaccac cgcaacacgg cggtggccat cgcccgccag ctgggcatct tcggccccga
     1381 ttccctggcc ctctccggcg aggaggtcga cgccctcgac gaccaggcgc tttttgaaaa
     1441 ggtcgaccgc atcccggttt acgcccgggt ttctccggaa cacaagctgc gcatcgtgcg
     1501 ggcctggcgc cgccggggtg aggtggtggc catgaccggt gacggggtca acgacgcgcc
     1561 ggcggtcaag gaggccagca tcgggatcgc catgggtatc gccggtaccg atgttaccaa
     1621 ggaagtggcc gacatggtca tcaccgacga taacttcgct tccatcgagg cggcggtcga
     1681 ggagggacgg ggcatctacg acaacatcca gaagttcatc cattacctgc tttcctgcaa
     1741 cgccggggag atcctggtga tgtttgccgc ttccctgatc gggctgccgg ttcccctgct
     1801 gccgatccag atcctctggg tgaacctggc caccgacggg ttcccggccc tggcgctcgg
     1861 cgtcgatccc ttcgacccgg acgtgatgga ccgtccgccg cgccccgcca acgcctcggt
     1921 cgtcacccgg gcccggatcc aggcactgct cctccagggc tcctttatcg ccttctgcag
     1981 cctgatggcc
//
LOCUS       DAOQYN010000276         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_68848_length_1949_cov_3.426610, whole genome shotgun sequence.
ACCESSION   DAOQYN010000276 DAOQYN010000000
VERSION     DAOQYN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68848_length_1949_cov_3.426610"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PKN80_08780"
     CDS             <1..376
                     /locus_tag="PKN80_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HNQ36140.1"
                     /translation="ASNRTIGQLALAPETLTGVIGLVSSGRITAATGKEVLRLAVETG
                     REPETIVSEQNLGQISDSDTLAGLVRSTLAANPKSVEDYRRGKTNAIGFLVGQVMRQT
                     KGKANPQLVQELLAGLLAEKKD"
     gene            429..1637
                     /locus_tag="PKN80_08785"
     CDS             429..1637
                     /locus_tag="PKN80_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNQ36141.1"
                     /translation="MKKRFKVLGMAVLLLVLALTVGENLAESQARKSEETLYQQLQMF
                     SDVLSIVQKNYVEEVENKKLINGALRGMLSSLDPYSQFLEPELYKELEIETEGKFGGV
                     GMTITVKDNLVTVVSPLEDTPAFRAGIQPNDRIIKIDGKLTRGMDSAAAAKLLRGDPS
                     TRVNIEVLREGAPELLKLELTRELIKLKSVKRAQLIEGSRIGYIRLVEFQKESYTDLK
                     AALETLRKDGLEGLVLDLRNNPGGLLDSSISIADLFLPPNRLVVYTQGRRTEDRIEYR
                     TRQAEAIPASLPMVILVDEGSASASEILAGALSDSKRAVLVGKKSFGKGSVQTVIPLP
                     ESSAVKLTIARYYTPSGRVIEGKGLTPDIEVEQNIRTLALEGKIDLEKDAQLQRALDL
                     LKGLTVLREK"
     gene            1634..>1949
                     /locus_tag="PKN80_08790"
     CDS             1634..>1949
                     /locus_tag="PKN80_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36142.1"
                     /translation="MSKVSGGPSGKNGHPLTPLLIILALLWGGLLLIRHPEVLHLNWP
                     RPPLREAKLKFPGRLDRARLEERVREPLARLSRPQPPLPSPAIETPALAIILDDFGYR
                     LGD"
BASE COUNT          406 a          641 c          613 g          289 t
ORIGIN      
        1 agcctcgaac cggaccatcg gtcaactcgc cctggcgccg gagacgctta ccggggtcat
       61 cggcctggtc tccagcgggc gcatcaccgc ggcgaccggc aaggaagtcc tccggctggc
      121 cgtcgagacc ggccgggaac ccgaaaccat cgtctccgag cagaacctgg gccagatatc
      181 ggattccgac accctggccg ggctggtccg ttcgaccctc gccgccaacc cgaaatcggt
      241 cgaggattac cggcggggca agaccaacgc catcggcttc ctggtcggcc aggtgatgcg
      301 ccagaccaag ggcaaggcca acccgcagct ggtccaggaa ctgctggccg ggctgctggc
      361 ggaaaagaag gattgaaaat caagatactc catcagtcaa accggctgaa tcagccaacg
      421 ggagccagat gaaaaaacgg ttcaaggttt tgggaatggc ggtcctgctg ctcgtcctgg
      481 ccctgacggt cggcgaaaac ctcgccgaaa gccaggcgcg caaatccgag gagacccttt
      541 accagcagct ccagatgttc agcgacgttc tctccatcgt ccagaagaac tacgtcgagg
      601 aggtggagaa caagaagctg atcaacgggg cgctccgggg catgctctcc tctctcgatc
      661 cgtacagcca gttcctggaa ccggagctgt acaaggagct ggagattgaa accgagggga
      721 aattcggcgg cgtcggcatg accatcaccg tcaaggacaa cctggttacg gtggtctcgc
      781 cgcttgaaga tacgcccgcc ttccgggccg gcatccagcc caacgaccgg atcatcaaga
      841 tcgacggcaa gctgacccgg ggcatggaca gcgccgcggc cgccaagctg ctgcgcggag
      901 acccgagcac cagggtcaac atcgaggtcc tgcgcgaggg ggccccggaa ctgctcaagc
      961 tggaactcac ccgggagctg atcaagctga agtcggtcaa gcgggcccag ttaatcgagg
     1021 ggtcccgaat cggctatatc cggctggtcg agttccagaa ggagagctat accgacctca
     1081 aggcggccct ggaaaccctg cggaaggacg gcctggaagg cctcgtcctc gacctgcgga
     1141 acaatccggg cggcctgctg gacagttcca tcagcatcgc cgatctcttc ctgccgccca
     1201 accgcctggt ggtttacacg cagggacggc gcaccgagga ccggatcgag taccgcaccc
     1261 ggcaggccga ggccatcccg gccagcctgc ccatggtcat cctggtcgac gagggcagcg
     1321 ccagcgcctc ggagatcctg gccggcgccc tttcggactc caagcgggcg gtcctggtcg
     1381 gcaagaaaag cttcggcaag ggatcggtcc agacggtcat cccgctgccg gaaagcagcg
     1441 cggtcaagtt gaccatcgcc cgctattaca cgccgagcgg acgggtcatc gagggcaagg
     1501 gcctcacccc cgacatcgag gtggaacaga acatccgcac cctggcgctg gaaggtaaga
     1561 tcgacctgga aaaggatgcc cagcttcagc gggccctcga cctgctgaaa gggctgaccg
     1621 tcctgcgcga aaaatgagca aggtcagcgg cggcccgtcc ggaaaaaacg gccatcccct
     1681 gacgccactc ctgataatcc tggcgctgct gtggggaggg ctcctgctga tcaggcatcc
     1741 ggaagtcctg cacctaaatt ggccgcggcc gcccctgcgc gaagcgaagc tgaagtttcc
     1801 cggccggctt gaccgcgccc ggctcgaaga gcgggtcagg gaaccgctgg cccggttaag
     1861 ccgcccgcag ccgcccctgc ccagtccggc catcgaaacg ccggccctgg ccatcatcct
     1921 cgacgatttc ggctaccggc tgggcgatt
//
LOCUS       DAOQYN010000277         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_69202_length_1943_cov_4.332097, whole genome shotgun sequence.
ACCESSION   DAOQYN010000277 DAOQYN010000000
VERSION     DAOQYN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69202_length_1943_cov_4.332097"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1943)
                     /locus_tag="PKN80_08795"
     CDS             complement(<1..>1943)
                     /locus_tag="PKN80_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36143.1"
                     /translation="AEGETEPVSVAGGQTVKRAVAIRPPLRGIFSATYGMAGRALPEG
                     ETVYLVMSRPSERPTRHELGANISLNPAELAVQSRLGLDWVLTCKTRELASADEGAHP
                     APGVWNWQDERAALPASYDMKLLPCFWPGHLPTFMVDPLDTAKYRTQRGAPRASRPKL
                     DLWAEHVGTVASHYKSLVDMWCVDDEVECSWTPDSYAPVVKTTVEAVRQAAPGVKIGL
                     SGTAEFTEELMRYVPPEQIDFFGASAFDYYYNEAKYAGGLKAKYGKPFICYGVGGRQP
                     DATMYHTLYTFRPPRSKAAWMARLVVYQTLLTDLDVIGHYAGVFRNDGSHLGLNKPLC
                     DYDATPYVWGGTFGCLGTLMADAELVEEVPLGPTDRLAFIFRTGRGLGAVTYATNTPQ
                     NDMHWKPAVREFRNLSLACPAGSVEALDMYWNPLTEAKWSGGKLRFDLNEEPVFIMNK
                     NLSETDFMDLFRKAEVDAPPVELAFEPKVDGAGRVALEVLVKNNTGRDLEGASVDFRI
                     PSNPSASINPFNTAGAWVAPLKVSAGRIGAGQTARLTVPTAYEARLPLELGYLRGVVT
                     ASGGAEYAGEDYLWLLPAPAAAAAPKADGDLAEWEGRPAAWLAYQRHFNFSCRWEQFL
                     EGGENFGYPSYTLDGRVAFWAAW"
BASE COUNT          301 a          704 c          640 g          298 t
ORIGIN      
        1 cccaggccgc ccagaaggcc acccgcccgt ccagcgtgta ggacgggtac ccgaaattct
       61 cgccgccctc caggaactgc tcccagcggc agctgaaatt gaagtggcgc tggtaggcca
      121 gccaggccgc cggccggcct tcccactcgg ccaggtcgcc gtcggccttc ggcgccgccg
      181 cggcggccgg cgccggcagc agccagagat aatcctcgcc ggcatactcg gccccgccgg
      241 aggccgtcac caccccgcgc aggtagccca gctccagcgg caggcgcgcc tcgtaggcgg
      301 tcggcaccgt cagccgcgcc gtctggccgg cgccgatccg gccggccgaa accttcaggg
      361 gcgccaccca ggccccggcg gtattgaagg ggttgatgga ggcgctcggg ttggaaggaa
      421 tccggaaatc gaccgacgcg ccttccaggt cgcggccggt gttgttcttt accagcacct
      481 ccagcgcgac ccggccggcc ccgtcaacct tcggctcgaa ggccagttca accggcgggg
      541 cgtccacttc ggccttccgg aagaggtcca tgaagtccgt ctcgctcagg ttcttgttca
      601 tgatgaagac cggctcctcg ttgagatcga agcgcagctt tccgcccgac cacttcgcct
      661 ccgtcagcgg gttccagtac atgtcgagcg cctcgacgct gccggccggg caggccaggg
      721 agaggttccg gaactcccgc accgccggct tccagtgcat gtcgttctgc ggggtgttgg
      781 tggcataggt aaccgcgccc aggccacgcc cggtccggaa gatgaaggcc agccggtcgg
      841 tcggaccgag cggcacctcc tcgaccagct cagcgtcggc catcagcgtc cccaggcagc
      901 cgaaggtccc gccccagacg taaggcgtgg cgtcgtaatc gcagagcggc ttgttcaggc
      961 ccaggtggga accgtcgttc cggaagacgc ccgcgtagtg gccgatcacg tccaggtccg
     1021 taagcagcgt ctggtaaacc accagccggg ccatccaggc cgccttggaa cggggcggcc
     1081 ggaaggtata aagggtatgg tacatggtcg cgtccggctg gcggccgccc accccgtagc
     1141 agatgaaggg cttcccgtac ttggccttca ggccgcccgc gtacttggcc tcgttatagt
     1201 agtaatcgaa ggccgaggcg ccgaagaagt cgatctgctc cggcggcacg taacgcatca
     1261 gctcctcggt gaattcggcc gtgccgctca gaccgatctt cacgcccggg gccgcctggc
     1321 ggaccgcctc gacggtcgtc ttcaccaccg gcgcgtagga gtccggcgtc caggagcatt
     1381 ccacctcgtc gtcgacgcac cacatgtcaa ccagcgactt gtaatggctg gccaccgtgc
     1441 ccacgtgctc ggcccagagg tcgagcttcg gccgcgaagc ccgcggcgcg ccccgctggg
     1501 tccggtattt cgcggtatcc agcggatcga ccataaaggt cggcaggtgg cccggccaga
     1561 agcagggcag gagtttcatg tcgtagctgg ccggcagggc cgcccgctca tcctgccagt
     1621 tccagacgcc cggcgccggg tgggccccct cgtcggccga ggccagttcc cgggtcttgc
     1681 aggtgagcac ccagtccagg cccagccgtg actggacggc cagctcggcc gggttgagcg
     1741 aaatgttggc gcccagttcg tggcgggtcg gccgctccga gggccggctc atcaccaggt
     1801 aaaccgtctc gccctccggc agcgcccggc cggccattcc gtaagtcgcc gagaagatcc
     1861 cccgcagcgg cggccggatc gccaccgccc gcttcaccgt ctggccgccg gccaccgaca
     1921 ccggctcggt ctcgccctcg gcc
//
LOCUS       DAOQYN010000278         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_69265_length_1942_cov_3.291468, whole genome shotgun sequence.
ACCESSION   DAOQYN010000278 DAOQYN010000000
VERSION     DAOQYN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69265_length_1942_cov_3.291468"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKN80_08800"
     CDS             <1..468
                     /locus_tag="PKN80_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36144.1"
                     /translation="VITPVSRAGDARAEIELLENGFRVRYTLKDLQGSGRGYFAFIPP
                     WYNIRYGSGRLHRREDGQWQAVTPSRYGPNNFGGYDIFEFQSGWGYRYTLDFSAGTGE
                     KVAGIHNWRYQENQGFLNGYHNLWPWTWEEGGRFTLDLKLTLQPEPGPENSKP"
     gene            465..1295
                     /locus_tag="PKN80_08805"
     CDS             465..1295
                     /locus_tag="PKN80_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ36145.1"
                     /translation="MKKENPMKKEALELFPAPEAGRLFRFIHISDTHLRSDGSFTGEL
                     VRVIEQINGSGADFVIHTGDIADDGAIGGLERFMKLVSGLKVPVRCVRGNHELEGEAG
                     REPGLYQRLVGQPLYYSFQHRGFRFIGLDTAAAEHMGAVSPEQLDWLEKELAAAGPAF
                     IFAHVPLRREWDQGPHRWYITENSERLAALLEKRPPAAFLAGHLHWTNVFTRSGPTLH
                     STAPSLGGLYNYPNRNGYLVWDVYPDRIIQYYKYLYLPWEPSIKNVVRLNGSDGTATR
                     "
     gene            1306..>1942
                     /locus_tag="PKN80_08810"
     CDS             1306..>1942
                     /locus_tag="PKN80_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36146.1"
                     /translation="MFNLFKRAKFYTGLGLLLLPALALSFATQARAGAYREDFESLTA
                     GASLSGQAGWTAESFVTVEEGTSHAGKKALRITPTPAAGRAFQRTLEWDAGWKEVWVD
                     AYLRTPKSPQLAMLEVQKGGQMMAAGGPGGPGEAGSGFVFRSSRGQNIGWPRMYERAS
                     NIYTGESSQLKPETWVRLTMRLDLEKKEFEVYRNGKCVFSEMEMAKALPAAG"
BASE COUNT          377 a          640 c          612 g          313 t
ORIGIN      
        1 gtcataaccc cggtcagccg ggccggcgac gcccgggccg agatcgagtt gctggaaaac
       61 ggcttccggg tccgttacac gctgaaggat ctgcaggggt ccggccgggg ctacttcgcc
      121 ttcatcccgc cctggtacaa tatccggtac ggaagcgggc gcctccaccg ccgcgaagac
      181 ggccaatggc aggcggtcac cccctcccgc tacggtccca acaatttcgg cggttacgac
      241 attttcgagt tccagagcgg ctggggatac cgttacaccc tggacttttc ggccggcacg
      301 ggcgaaaaag tggccggcat ccataactgg cgctaccagg agaaccaggg cttcctgaac
      361 gggtatcaca acctgtggcc ctggacctgg gaggagggcg gccgctttac cctcgacctg
      421 aaactcaccc tccagccgga gcccggcccg gagaattcga aaccatgaaa aaagagaacc
      481 cgatgaaaaa ggaggccctt gaactcttcc cggcgccgga ggccggacgc ctcttccgtt
      541 tcatccacat ctccgatacc catctccgtt cggacggcag tttcaccggg gaattggtcc
      601 gggtcatcga gcagataaac ggttcggggg ccgatttcgt cattcacacc ggcgacatcg
      661 ccgacgacgg ggccatcggc ggcctggagc gcttcatgaa actggtctcc ggcctcaagg
      721 tgccggtccg ctgcgtccgg ggcaaccacg agctggaagg cgaggccggc cgggagccgg
      781 gcctttacca gcggctggtc ggccagccgc tctattactc cttccagcac cggggcttcc
      841 gtttcatcgg cctggacacc gccgccgccg aacacatggg agcggtcagc ccggaacaac
      901 tcgactggct ggaaaaggaa ctggcggcgg ccgggccggc cttcattttc gcccacgtgc
      961 cgctccggcg cgaatgggat cagggaccgc accgctggta tatcaccgaa aacagcgaac
     1021 ggctggccgc cctgcttgaa aaaaggccgc cggccgcctt cctggccggt cacctgcact
     1081 ggaccaatgt cttcacccgg tccggaccga ccctgcactc caccgcgccc agcctgggcg
     1141 gcctctataa ttatccgaac cgcaacggct acctggtctg ggatgtttac ccggaccgga
     1201 tcatccagta ctacaagtac ctctaccttc cctgggaacc aagcataaaa aacgtggtca
     1261 gactgaacgg ttccgatgga accgcaacca gataaggagg atggaatgtt taatttattt
     1321 aagcgcgcga agttttacac cggtctcggc ctgcttctgc tgccggcact ggcgctctcc
     1381 tttgcgaccc aggcccgggc cggggcttac agggaggatt tcgagtccct gaccgccggc
     1441 gcgtccctct ccggccaggc cggctggacg gccgagagct tcgtcaccgt ggaggagggg
     1501 accagccacg ccggcaagaa ggcgctccgg atcactccga cgccggccgc cggacgggcc
     1561 ttccagcgga ccctcgagtg ggacgccggc tggaaggaag tctgggtcga cgcctacctg
     1621 cgcacgccca aaagccccca gctggccatg ctggaggttc agaagggcgg ccagatgatg
     1681 gccgccggcg gaccgggcgg accgggcgag gccggcagcg gcttcgtctt ccgcagcagc
     1741 cgcggacaga acatcggctg gccgcgcatg tacgaacggg cctcgaacat ctacacgggc
     1801 gagagcagcc agttgaagcc ggagacctgg gtgcggctga caatgcggct ggacctggag
     1861 aagaaggagt tcgaggtcta ccgaaacggc aaatgcgtct tctccgaaat ggagatggcc
     1921 aaggccctgc cggccgccgg cg
//
LOCUS       DAOQYN010000279         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_69671_length_1935_cov_4.011170, whole genome shotgun sequence.
ACCESSION   DAOQYN010000279 DAOQYN010000000
VERSION     DAOQYN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69671_length_1935_cov_4.011170"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /gene="rlmN"
                     /locus_tag="PKN80_08815"
     CDS             <1..938
                     /gene="rlmN"
                     /locus_tag="PKN80_08815"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNQ36147.1"
                     /translation="QVFNWLYRKGAEDFASMTNLPAALRHRLTESFSIVRPVAAERRR
                     AADGTEKILFRLAGGGGVETVLIPSPPRLTLCLSTQFGCRFGCVFCASGRIGFKRNLT
                     PSEIVGQVLQVQFREKRPVTNLVFMGMGEPFDNYENLTAAIRILNHPHGLNIGARKLT
                     VSTCGLVPQIEAFRELGLQVELSISLHAADEELRSRLMPVNRRYPLAELLPAAARYTR
                     ETGRQLTLEYLLMAGVNDGPEAARQLAETARRLGAKVNLINFNPVPGLPFKPAPEPAA
                     AAFQKGLRAAGVNVTRRRSRGAGVGAACGQLAGES"
     gene            935..1642
                     /locus_tag="PKN80_08820"
     CDS             935..1642
                     /locus_tag="PKN80_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNQ36148.1"
                     /translation="MKKEGPFRAVIFDIDGTITRPVSSWRYLHERLGKWDALACRYQE
                     QFLAGRISYRRFCHLDAAHWAGRSDSEMEGIFDAVPYARYAVPCLKKLKQTGLKLAAV
                     STGLQYLPRRLQRELGFDAVLCNELVVGRDGRLTGEVKINITHGAKGRALGRILKELE
                     VKPAEVICVGDSEGDIPMARAVGYSIAFNSSSPELSDLVDYNCRSDDFREVMRCILTR
                     LTGSGGRKLQTAGPSGV"
     gene            1652..>1935
                     /locus_tag="PKN80_08825"
     CDS             1652..>1935
                     /locus_tag="PKN80_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HNQ36149.1"
                     /translation="MAEPEKTGRKQARRLTFTGRGAPPVSVAWKRGPAAPDNYLNYHR
                     EPELQFIKRGAGAYLIEGRRHPFRKNSLLLIRPNQVHRIIASPGLFIERAT"
BASE COUNT          346 a          679 c          599 g          311 t
ORIGIN      
        1 tccaggtctt caactggctc taccggaagg gtgccgaaga ctttgcctcg atgaccaacc
       61 tgccggccgc tctccgccat cggctgacgg agagcttctc catcgtccgg ccggtggcgg
      121 cggaacggcg ccgggccgcc gacggaaccg aaaagatact cttccggctg gccggcggcg
      181 gcggcgtgga aaccgtgctg attccaagcc cgccgcgcct gacgctctgc ctttcaaccc
      241 agttcggctg ccgtttcggc tgtgtcttct gcgccagcgg ccggatcggt ttcaagcgaa
      301 acctgacccc ctcggaaatc gtcggacagg tactccaggt acagttccgg gagaagcggc
      361 cggtgaccaa cctggtcttc atgggcatgg gtgagccctt cgacaattac gagaatctga
      421 ccgccgccat ccggatcctg aatcatccgc acggcctgaa tataggcgcc cggaagctga
      481 ccgtctccac ctgcggcctg gtcccgcaga tcgaggcctt ccgggagctc ggcctccagg
      541 tcgaattatc aatctccctc cacgccgccg atgaggaact gcgaagccgc ctgatgccgg
      601 tgaaccgccg ttacccgctc gccgagctgc tgccggccgc ggcccgctac accagagaga
      661 ccggccgcca gctgaccctc gagtacctgc tgatggccgg ggtcaacgac ggaccggagg
      721 cggcccggca gctggccgag acggcccggc ggctgggggc caaggtcaat ctcatcaact
      781 tcaacccggt ccctggcctg cccttcaagc cggcgccgga accggcggcg gccgcctttc
      841 agaaggggct ccgggccgcc ggggtaaacg tcacccggcg ccggagccgg ggcgccgggg
      901 tcggggccgc ctgcggacag ctggccggag aatcatgaaa aaagaaggcc ctttccgggc
      961 ggtcatcttc gatatcgacg gtaccatcac ccgcccggtc agttcctggc gttacctcca
     1021 cgagcgcctc ggcaaatggg acgcgctcgc ctgccgctac caggagcaat tcctggccgg
     1081 ccgcatctcc taccgacgct tctgccacct ggacgccgcc cactgggccg gccggagcga
     1141 ctcggaaatg gaaggcatct tcgacgcggt cccctatgcc cgttacgcgg tcccctgtct
     1201 caagaagctc aagcagaccg gcctcaagct ggcggcggtc tctaccggtc ttcagtacct
     1261 gccccgccgc ctccagcggg aactgggatt cgatgccgtc ctctgcaacg aactggtggt
     1321 cggccgggac ggccggctca ccggcgaggt aaagatcaac atcacccacg gcgccaaggg
     1381 ccgggcgctg ggccggatac tcaaggaact ggaagtaaag ccggccgagg tgatctgcgt
     1441 gggcgacagc gagggcgaca tcccgatggc ccgggccgtc ggctactcga tcgccttcaa
     1501 ctcctcctca ccggaactct cggacctggt tgattacaac tgccggagcg acgacttccg
     1561 cgaggtgatg cgctgtattt taacccggtt gaccggctcc ggcggccgga aacttcaaac
     1621 ggccggccct tccggagtat aatcaaaatc catggccgaa cctgaaaaaa ccggccggaa
     1681 gcaggcccgg cgcctgactt ttaccggccg cggcgccccg ccggtttcgg tcgcctggaa
     1741 acggggaccg gccgcgccgg acaattacct gaattaccac cgggaaccgg aactgcagtt
     1801 catcaagcgc ggcgccgggg cctacctgat cgaagggcgg cgccacccct tccggaaaaa
     1861 cagcctgctg ctgatccgtc ccaaccaggt acaccgcatc atcgcctctc ccgggctttt
     1921 catcgagcgg gcgac
//
LOCUS       DAOQYN010000280         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_69785_length_1933_cov_3.214058, whole genome shotgun sequence.
ACCESSION   DAOQYN010000280 DAOQYN010000000
VERSION     DAOQYN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69785_length_1933_cov_3.214058"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PKN80_08830"
     CDS             <1..796
                     /locus_tag="PKN80_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNQ36150.1"
                     /translation="SGGRLGILLLAELAPDRARVLIRGRVHPGLVLPLDEASRLEILQ
                     RLEAGAWEVRFHGRSAAETLARHGEVPLPPYIRRPARASDRADYQTVYARREGAVAAP
                     TAGLHFTDGLLKELEDRGVGRAFLTLHTGWGTFQPLRSETVEAHRMDPEYFELPAETA
                     AAVNKARQEKRPVTAVGTTVVRTLEGRGGEGRLEAAAGWVNAFIYPGYRFRIVDAMVT
                     NFHLPRTTTLLLTAAFAGPDRLLAAYRFALERGFRFGSYGDAMLIT"
     gene            806..1915
                     /gene="tgt"
                     /locus_tag="PKN80_08835"
     CDS             806..1915
                     /gene="tgt"
                     /locus_tag="PKN80_08835"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNQ36151.1"
                     /translation="MKFEITTLDPGSAARAGRIETGHGAVETPVFLPVATRGSIRCLV
                     SEELEELGYRMLLANAYHLALRPGTEVVAAAGGLHRFMNWPRPILTDSGGFQIFSLAE
                     ARVEEAGVVFRSVYDGRRHRLSPEEAVRIQEELGADIIMTLDQPVSHPADRETAAEAV
                     RRTTRWAKRCQAGRQRPEASALFGIVQGSVYPELRAESAAALVELDFPGYALGGFCLG
                     EPLELMNEVVAAVTGRLPAEKPRYLMGIGTPREIISAVAAGVDIFDCAMPTHIARNGA
                     ALTAAGRLTIRNGRYRNDHGPLDPACGCRVCRRYSRAYLRHLFNTRELTAFNLLTYHN
                     LHFMRGLMAGLRAAIEAGRLGSFRKSFVDGPAAGL"
BASE COUNT          282 a          697 c          693 g          261 t
ORIGIN      
        1 cagcggcggc cggctgggaa tcctgctcct ggccgagctg gcgccggacc gggcccgggt
       61 cctcatccgg ggccgggtgc acccgggcct ggtgctgcca ctggacgagg ccagccggct
      121 cgagatcctc caacggctgg aggcgggggc ctgggaggtg cgcttccacg gccgcagcgc
      181 ggccgagacg ctggcccgcc acggcgaggt gccgctgcct ccttatatcc ggcggccggc
      241 ccgggccagc gaccgggccg actaccagac cgtttacgcc cggcgcgagg gggcggtggc
      301 cgcgccgacc gccggactgc acttcaccga cgggctgctg aaggaactgg aagaccgggg
      361 cgtcggtcgg gcctttctca ccctgcacac cggctgggga accttccagc cgctgcgcag
      421 cgagaccgtc gaggcgcacc ggatggaccc ggaatacttc gagctgccgg ccgaaacggc
      481 ggccgccgtc aacaaggccc ggcaggaaaa gcggccggtg acggcggtcg gcaccaccgt
      541 ggtccggacc ctggagggaa ggggcgggga ggggcgcctg gaggcggccg ccggctgggt
      601 caacgccttc atctacccgg gctaccggtt ccggatcgtc gacgccatgg tgaccaactt
      661 ccacctgccg cgcaccacca cgctcctgct gaccgccgcc ttcgccggcc cggaccggct
      721 gctggcggcc taccggttcg ccctggaacg gggcttccgt ttcggcagtt acggcgacgc
      781 catgctgatc acctgaaccg aaaccgtgaa attcgaaatc accaccctgg accccggcag
      841 cgccgcccgg gccggccgga tcgaaaccgg ccacggcgcg gtggagacgc cggtcttcct
      901 gccggtggcc acccgcggca gcatccgttg cctggtctcg gaggaactcg aggagctggg
      961 ctaccggatg ctgctggcca acgcctacca cctggccctg cggccgggaa cggaggtggt
     1021 ggcggccgcg ggcgggctgc accgcttcat gaactggccg cggccgatcc tgaccgacag
     1081 cggcggcttc cagatcttca gcctggccga ggccagggtc gaggaggccg gcgtggtctt
     1141 ccgctcggtc tatgacggcc ggcgccaccg gctcagcccg gaggaggcgg tccggatcca
     1201 ggaggagctg ggcgcggaca tcatcatgac cctggaccag ccggtctccc acccggccga
     1261 ccgggagacg gcggccgaag cggtgcggcg gaccacccgc tgggcgaagc gctgccaggc
     1321 cggccggcag cggccggagg cctcggccct cttcgggatc gtccagggct cggtctaccc
     1381 ggaactgcgg gccgagtcgg cggcggccct ggtggagctg gattttcccg gctacgccct
     1441 gggcggcttc tgcctgggcg aaccgctgga actgatgaac gaggtggtgg ccgccgtcac
     1501 cgggcggctg ccggccgaaa aaccgcgcta cctgatgggc atcgggacgc cgcgggagat
     1561 catctcggcg gtggccgccg gcgtggacat cttcgactgc gccatgccca cccacatcgc
     1621 ccgcaacggg gcggccctga ccgccgccgg ccggctgacc atccgcaacg ggcgttaccg
     1681 gaacgaccac ggcccgctgg acccggcctg cggctgccgg gtctgccgcc gctactcccg
     1741 ggcctacctg cgccacctct tcaacacccg ggaactgacc gccttcaacc tgctgaccta
     1801 ccataacctg cacttcatga gaggcctgat ggccggcctg cgggcggcca tcgaggccgg
     1861 ccggctcggg agtttccgca aatcatttgt ggacggaccg gcggcggggc tataatataa
     1921 ggcagtaact tcc
//
LOCUS       DAOQYN010000281         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_71438_length_1906_cov_3.820097, whole genome shotgun sequence.
ACCESSION   DAOQYN010000281 DAOQYN010000000
VERSION     DAOQYN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71438_length_1906_cov_3.820097"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKN80_08840"
     CDS             <1..663
                     /locus_tag="PKN80_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ36152.1"
                     /translation="GFTLIELLVVIAIIAILAAMLLPALSRARAKARQASCMNNLKQL
                     YIINYLYWQDNEFFVYRAFVSPADVGRPAWPKALIQENYLKNPEMLRCPGWEVKKTQT
                     VSQTGAGNMNFYTGDLTYFCDYGWNSNFPGLQNGSVKTLTKESKEHRVIMLTESMIRG
                     NTDTELRGQTSSFAAIQAPAPAYYSQSPTYQIHIGGYNYLFFDGHVEYMPRNNSTPYW
                     TP"
     gene            754..>1906
                     /locus_tag="PKN80_08845"
     CDS             754..>1906
                     /locus_tag="PKN80_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36153.1"
                     /translation="MKKRVRPGQKTVLLSAAAVALLLAWTAAAAGQGRTETAYRRFIE
                     VENFRIEGKGHQVRAHENSSGGATMDSVFPDTGRLEAGGRLLFTLETPRPAGRYLVWL
                     NTLPLAGSRTAAGPIRIEAGLGRTAGMIEIRRNARYNWGYAFIEARESFQEISLKVRS
                     LEDRLWLDCLYLSNQPADLKANGRDGRPEINLKAIRVAAAGRSGSAAGEKTANRLANG
                     GFEAGLGSYYWSTLYQQSYALKPEFWDRQEAYEGSASLRLTLFPEVGRRLGDDPGLPT
                     AFRLMHRSLELEPDRAYCFRAMVKADTPLLVRFSASPAQPPETGRIRPAGQAILQADG
                     QWRPLEAELKTLGAGQDYLLRLEAEATGPARLWLDALTLTDTRLEAFRPA"
BASE COUNT          342 a          688 c          598 g          278 t
ORIGIN      
        1 ggctttaccc tgatcgaact gctggtggtg atcgccatca tcgccatcct ggccgccatg
       61 ctgctgccgg ccctctcccg ggcccgggcc aaggccaggc aggcctcctg catgaacaac
      121 ctgaagcagc tctacatcat caattatctc tactggcagg acaacgagtt cttcgtctac
      181 cgcgccttcg tcagcccggc tgacgtcggc cggccggcct ggccgaaggc cctcatccag
      241 gagaattacc tgaagaaccc ggagatgctc aggtgcccgg gctgggaggt caagaagacc
      301 cagacggtca gccagaccgg ggccggcaac atgaacttct acaccggcga ccttacttac
      361 ttctgcgact acggctggaa ctccaacttc ccgggccttc agaacggcag tgtcaagacc
      421 ctgaccaagg agagcaagga gcaccgggtg atcatgctta ccgagagcat gatccggggc
      481 aacaccgaca ccgagctgcg tggccagaca tccagtttcg cggccatcca ggcgccggcc
      541 cccgcttact acagccagag cccgacctac cagatccaca tcggcggtta caactacctc
      601 ttcttcgacg gccacgtgga gtacatgccg cgcaacaaca gcactcccta ctggacgccc
      661 tgaagagacc gggcttcccg gaagcgtatc ggccggaggg ggggcccggg gcctcccctc
      721 cggttttttt aaacgatcca aggagaaacc gccatgaaaa aaagagtccg gcctggtcaa
      781 aaaaccgtgc tcctttcggc ggccgccgtc gcgctgctgc tggcctggac ggccgcggcc
      841 gccggccagg gccggaccga gacggcctac cgccgcttca tcgaggtgga gaacttccgc
      901 atcgaaggca agggccacca ggtccgcgcg cacgagaaca gcagcggcgg cgccaccatg
      961 gactcggtct ttcccgatac cggccggctg gaggccggcg gccgccttct cttcaccctg
     1021 gagacgcccc ggccggccgg ccgctacctg gtctggctca ataccctgcc gctggccggc
     1081 agccgcaccg ccgccgggcc gatccggatc gaggccggcc tgggccgaac ggccgggatg
     1141 atcgagatcc gccggaacgc ccgttataac tggggctacg ccttcatcga ggcccgggaa
     1201 tccttccagg agatctccct gaaggtccgc agcctggagg accgcctctg gctcgactgc
     1261 ctctatctct ccaaccagcc ggccgacctc aaggcgaacg gccgggatgg ccggccggag
     1321 atcaacctca aggcgatccg cgtcgcggcg gccggccgga gcgggtccgc ggcgggagaa
     1381 aaaacggcca accggctggc caacggcggc ttcgaggccg gccttggaag ttactactgg
     1441 tcgaccctct accagcagtc ctacgccctc aagccggagt tctgggaccg gcaggaggcc
     1501 tatgaaggtt cggcctccct ccggctgacc ctcttcccgg aagtcggccg gcgcctgggc
     1561 gacgacccgg gactgccgac cgccttccgc ctcatgcacc gcagcctgga gctggaaccg
     1621 gaccgggcct actgcttccg ggccatggtc aaagccgaca cgccgctgct ggtcaggttc
     1681 agcgcctcgc cggcccagcc gccggagacc ggacggatcc ggccggccgg ccaggccatc
     1741 ctccaggccg acggccagtg gcggccgctc gaggccgaac tgaagaccct gggcgccggc
     1801 caggattacc tgctgcgcct cgaggccgag gcgaccgggc cggcccgact atggctggac
     1861 gcgctgacgc tgaccgacac ccggctcgag gccttccggc cggcgg
//
LOCUS       DAOQYN010000282         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_71914_length_1898_cov_4.395008, whole genome shotgun sequence.
ACCESSION   DAOQYN010000282 DAOQYN010000000
VERSION     DAOQYN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71914_length_1898_cov_4.395008"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PKN80_08850"
     CDS             <1..541
                     /locus_tag="PKN80_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNQ36154.1"
                     /translation="AAGVQPLAIDPAAAVAPPAAEPLPGAPLAAEGVPAGPALGPEPL
                     DPDFTPARWRRILAGRKARIKSLLLDQQAVAGLGNIYAAEALFRAAIRPDRPAGSLSG
                     AEAGRLYRSMRAVLKEALAAGGTSIQDYRQADGRSGLFQVSLRVYGRRGEACPCCGAP
                     IEACREGSRTTYFCPGCQH"
     gene            557..1540
                     /gene="tilS"
                     /locus_tag="PKN80_08855"
     CDS             557..1540
                     /gene="tilS"
                     /locus_tag="PKN80_08855"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNQ36155.1"
                     /translation="MKIEARVYGFIREHGLVGRGDRLLVAVSGGPDSAALFHLLRTLR
                     PRLQCSLFMAHFDHGLRPGSAADLEFCAALARKHRVPFVSERARLKAGAGEDAARRAR
                     YDFLARAAGSLSCHAVAVGHQLDDQAETLLLHLLRGSGLQGLAAMRPSRPLLPGSPVR
                     LVRPLLAESRADLLAYLAAGRHQWRLDESNLEAKYRRNRLRLEVMPLLESFNPRLKEA
                     LGRLAETAAADHDFLAGAAAGFLAGREREKDYFSLDAAAFNKLHPALQREALRRLVSE
                     LLGSQHRADYAGIEAARRFCLAASGREKTVAGRVRVSRRDGRLLFKAAGER"
     gene            1537..>1898
                     /locus_tag="PKN80_08860"
     CDS             1537..>1898
                     /locus_tag="PKN80_08860"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNQ36156.1"
                     /translation="MKTEKLKRLRRFFTALTALLLGVSCLAAAPEYPSPSGAVNDFAW
                     TLPEGERQELEALAGRIESETGAEIGVAIIPSLEGADLEGYANELFNRWGIGKKEKNN
                     GILILVAMAEHKIRIEVG"
BASE COUNT          243 a          747 c          619 g          289 t
ORIGIN      
        1 cgccgccggg gtgcagccgc tggcgatcga tccggccgcc gctgttgcgc cgccggctgc
       61 cgaacccctc cccggcgcac cgctcgctgc cgaaggggtc ccggcggggc cggccctcgg
      121 cccggagccg ctggatccgg atttcacccc ggcccgctgg cgccgcatcc tggccggccg
      181 caaggcccgc atcaagtccc tgctgctcga ccagcaggcg gtggccggcc tgggcaacat
      241 ctacgcggcc gaggccctct tccgggccgc catccggccc gaccggccgg ccggctccct
      301 ctccggcgcc gaggccggcc gcctttaccg ttccatgcgc gcggtgctca aggaggccct
      361 ggccgccggc ggcacctcga tccaggatta ccggcaggcc gacggccgca gcggcctctt
      421 ccaggtctcc ctgcgcgtct atggccgccg cggcgaagcc tgtccctgct gcggcgctcc
      481 catcgaggcc tgccgggagg gcagccggac cacctacttc tgccccggct gccagcattg
      541 aaattacttc ccggccatga agattgaagc gcgggtatac gggttcatcc gggaacacgg
      601 cctggtcggc cgcggcgacc gcctcctggt cgcggtctcc ggcggtcccg attcggccgc
      661 cctcttccac ctgctgcgga ccctgcgtcc ccgcctccag tgctccctct tcatggccca
      721 ttttgaccac ggcctgcgtc ccggctcggc ggccgacctc gaattctgcg ccgccctggc
      781 ccggaagcac cgggtcccct tcgtttccga acgggcccgg ctcaaggccg gcgccggcga
      841 ggatgccgcc cgccgggccc ggtacgattt cctggcccgg gccgccggga gtctctcctg
      901 ccacgcggtc gccgtcggcc accagctcga cgaccaggcc gagacgcttc tcctgcacct
      961 cctgcgcggc agcggcctcc agggcctggc cgccatgcgc ccctcccggc cgctgcttcc
     1021 cggctcgccg gtccggctgg tccgccccct gctggccgag tcccgcgccg atctcctggc
     1081 ttacctggcc gcgggccggc accaatggcg gctcgacgag agcaacctcg aggcgaagta
     1141 ccgccgcaac cgcctgcgcc tcgaggtgat gccgctgctc gaatcattca acccgcgcct
     1201 caaggaggcc ctcggccggc tggccgaaac cgccgccgcc gatcacgatt tcctggccgg
     1261 ggcggcggcc ggtttcctgg ccggccggga acgggaaaag gattacttct cccttgacgc
     1321 cgccgccttc aacaagcttc acccggccct ccagcgcgag gcgctccgcc ggctggtctc
     1381 tgagctgctc ggctcccaac accgggccga ttatgccggg atcgaggcgg cccgccgttt
     1441 ctgcctggcc gcttcgggcc gggagaagac cgtcgccggc cgggtccggg tttcgcgccg
     1501 ggacggccgg ctgctcttca aagccgcggg tgagagatga aaacggagaa actgaagcgc
     1561 ctccgccgct ttttcacggc cctgaccgcc ctgctcctcg gcgtctcctg cctggccgcc
     1621 gccccggagt acccctcccc gtccggcgct gtcaatgatt tcgcctggac cctcccggag
     1681 ggtgagcgcc aggaactgga ggcgctggcc ggccggatcg aatcggagac aggcgccgag
     1741 atcggcgtgg ccataatccc cagcctcgag ggggccgacc tggagggcta cgccaacgag
     1801 ctcttcaatc gctggggtat cggcaagaag gagaagaaca acggcatcct catcctggtg
     1861 gccatggccg agcacaagat ccggatcgag gtcggcta
//
LOCUS       DAOQYN010000283         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_72866_length_1883_cov_3.660284, whole genome shotgun sequence.
ACCESSION   DAOQYN010000283 DAOQYN010000000
VERSION     DAOQYN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72866_length_1883_cov_3.660284"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PKN80_08865"
     CDS             <1..755
                     /locus_tag="PKN80_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36157.1"
                     /translation="LDYAAAGGTLVTGPWFGLFDEWGEPAAGRRFDKNLFPFGSEFPA
                     WPELAGRPELSCTGPDRKRFKARPLAGLPSGSGRRVSLDFRAGNPAWRRTRVGRGQVV
                     QLAVDLGTLFRYSGAAAVVRGWKGLLENLEVKRPLVENLSGDGLLLGTFRGKPGETVV
                     HVQQFTPPWRPDDINVRAPGLRSKVRLRWNGARPLSVRGAVPELGPELGARRQGGAWL
                     IELPPFTWGQVVLVKTGPSRTKPKPTVARRSA"
     gene            752..>1883
                     /locus_tag="PKN80_08870"
     CDS             752..>1883
                     /locus_tag="PKN80_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36158.1"
                     /translation="MIIRLLGCFLLPLFLLSSVVPAWGAPYRRFIEAESMLAEGNGYR
                     VVQDKDSSGGAAVEVLLADTGLLAAGGRIYKNLEERLPAGEYSGWIQVRIPSVRPAGA
                     APLRLEVRFGGASGVVELDPGRPAPTGRLRLKVAAPFQEVSIKTVSPAPAFRIDRIYL
                     SSEARDIRLNPVTGDHEVDYDYILNASAGPVPEAQFGFVRLADPEAAQPANWLANGGF
                     EAGLGTTQWSTHYQQSYALKPEFWDRQEAYEGSASLRLTLFPVGWRLQGDRPDLPTSF
                     QMMSRTLKLPTGRSYYFRGMARSDVPATLLLTAETPYNPAVLARVSVQVDTGWRPFEM
                     EVKDLKDPRGCILYLAAGAAGPARLWLDALTLTDTRLEAFRPA"
BASE COUNT          255 a          664 c          652 g          312 t
ORIGIN      
        1 cgctcgatta cgcggccgcc ggcggcaccc tggtcaccgg cccctggttc ggcctcttcg
       61 acgagtgggg cgaaccggcc gccggccgcc ggttcgataa gaatcttttt cccttcggtt
      121 cggaatttcc cgcctggccg gaactggccg gccggccgga actctcctgc accggcccgg
      181 accggaagcg gttcaaggcc cggccgctcg ccgggctgcc gtccggatcc ggccgccggg
      241 tatcccttga tttccgggcc ggcaacccgg cctggcggcg cacccgggtg ggccggggcc
      301 aggtggtcca gctggccgtg gacctgggca ctctcttccg ttacagcggc gcggcggcgg
      361 tcgtccgggg ctggaaagga ctgctggaga acctggaggt gaaacggccc ctggtggaga
      421 acctttccgg ggacggcctg ctgctgggca ccttccgggg gaagcccggc gagacggtgg
      481 tccacgtcca gcagttcact ccgccctggc ggccggatga tatcaatgtc cgggcgcccg
      541 gcctccgttc gaaggtccgg ctgcgctgga acggagcccg tccgctctcg gtccgcggcg
      601 cggtgccgga attggggccg gagctgggcg cgcgccggca gggcggcgcc tggctgatcg
      661 agctgccgcc cttcacctgg ggacaggtcg tcctggtcaa aaccggtccg tcccggacca
      721 aaccaaagcc aaccgtggcg aggagatcgg catgattata agattattag gctgtttcct
      781 gcttccgctg tttctgctgt cgtccgtcgt tccagcctgg ggggcgccct accgccgctt
      841 catcgaggcg gaatcaatgc tggcggaggg gaacggctac cgggtggtcc aggataagga
      901 ctccagcggc ggcgccgcgg tcgaggtgct cctggccgat accgggctcc tggccgccgg
      961 cggccggatt tacaagaacc tggaggagcg gctgccggcc ggcgagtact ccggctggat
     1021 ccaggtgcgg attccgtccg tccgtccggc cggggccgcc ccgctccggc tggaagtgcg
     1081 gttcggcggc gcctccggcg tggtggaact cgatcccggc cggccggcgc cgaccggccg
     1141 gctccggctc aaggttgcgg cccccttcca ggaggtttcg ataaaaacgg tcagtccggc
     1201 cccggccttc cggatcgacc ggatctacct gtccagcgag gcccgcgata tccgcttgaa
     1261 tccggttacc ggcgaccacg aggtggatta cgattacatc ctcaacgctt cggccggtcc
     1321 ggtccccgag gcgcagttcg gtttcgtccg gctggccgat ccggaggccg cccagccggc
     1381 caactggctg gccaacggcg gcttcgaggc cggcctcgga accacgcaat ggtcaaccca
     1441 ctaccagcag tcctacgccc tcaagccgga gttctgggac cggcaggagg cctatgaagg
     1501 ttcggcctcc ctccggctga ccctcttccc ggtcggctgg cgcctgcagg gcgaccggcc
     1561 ggacctgccg accagcttcc agatgatgag ccggacgctc aagctgccga ccggccgctc
     1621 ctattatttc cgggggatgg ccaggtcgga tgtcccggcc acccttctcc tgacggccga
     1681 gaccccctac aacccggccg tcctggcccg ggtctcggtc caggtggaca ccggctggcg
     1741 cccctttgaa atggaggtca aggaccttaa ggatccgcgc ggctgcattc tctacctggc
     1801 ggccggcgcc gccgggccgg cccgactctg gctggacgcg ctgacgctga ccgacacccg
     1861 gctcgaggcc ttccggccgg cgg
//
LOCUS       DAOQYN010000284         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_73531_length_1873_cov_4.579758, whole genome shotgun sequence.
ACCESSION   DAOQYN010000284 DAOQYN010000000
VERSION     DAOQYN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73531_length_1873_cov_4.579758"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PKN80_08875"
     CDS             complement(<1..372)
                     /locus_tag="PKN80_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36159.1"
                     /translation="MSAPRRVAIGGRVLKPGRPRLVGVAAGPGWEASDWSRADILEIR
                     LDHFPEVRDGVAALGRPGIRVWGEPGRALVAQGVSVVVQVQHRRGAELFINDGIYGSL
                     SDAGAPGFRFPVRLVGRQSDAA"
     gene            complement(383..1048)
                     /locus_tag="PKN80_08880"
     CDS             complement(383..1048)
                     /locus_tag="PKN80_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HNQ36160.1"
                     /translation="MKLVDLHTHSLLSDGLYLPMELARRAECRGYRWLAITDHVDASN
                     LERVIPEVLKAAAEINRRSAGFRVIPGAEVTHAPAGSIAGLVDAARRLGARLVLVHGE
                     TLAEPVVPGTNRAAIEAGPDILAHPGLIDAADYRRAAEKGVRLEISARKGHCLANGHL
                     VQLWRRHGGRLVIDTDGHQEDDLFTEARYLGVGRGAGLTEPEVRAILKESLAFAAGRD
                     KTA"
     gene            complement(1096..1449)
                     /locus_tag="PKN80_08885"
     CDS             complement(1096..1449)
                     /locus_tag="PKN80_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNQ36161.1"
                     /translation="MAKENLGNVKIEHEVLAAIAAAAAGKVSGVSRIIPGFVGGLAEL
                     FGRSAPQHSVKVTVREECLFFELAVEVLYGKDVPQLAWEVQKAVKEAVEAMTGMRVAK
                     VDVEVRGLKVVPEKA"
     gene            complement(1525..>1873)
                     /locus_tag="PKN80_08890"
     CDS             complement(1525..>1873)
                     /locus_tag="PKN80_08890"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852790.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNQ36162.1"
                     /translation="DPAMNFRPSPGTVNRLLVPGGPGVRFDSHLYQGYRVPPYYDSLI
                     GKLIVSGRDRREALERLRRALMELEIDGVKTTVPLYRKLLDHAGFLSGKYYVGLIETM
                     LEKEQSDESAAGQ"
BASE COUNT          289 a          691 c          613 g          280 t
ORIGIN      
        1 cgcggcgtcg gactgccggc cgaccagacg caccgggaag cggaacccgg gcgcgccggc
       61 atcggacagg ctgccgtaga tgccgtcgtt gatgaacagc tccgccccgc ggcggtgctg
      121 cacctggacc acgaccgaga cgccctgcgc caccagcgcg cggccgggct cgccccagac
      181 gcggatgccg ggccggccga gggcggccac gccgtcgcgg acctccggaa agtggtcgag
      241 ccggatctcc aggatgtcgg cccgggacca gtcggacgcc tcccagccgg gaccggccgc
      301 cacgccgacc aggcggggcc ggccgggctt cagaacccgg ccgccgatag ccacccttcg
      361 cggagcgctc atgatctcga attcaggccg tcttgtcccg gccggcggcg aaggccaggc
      421 tctccttcag gatcgcccgg acctccggct cggtcaggcc ggcaccgcgg ccgacgccca
      481 ggtaacgcgc ctcggtgaaa aggtcatcct cctggtggcc gtcggtgtcg atgaccaggc
      541 ggccgccgtg gcggcgccag agttgaacca ggtggccgtt ggccaggcaa tggcccttgc
      601 gggcggatat ctccaggcgc accccttttt cggccgcccg ccggtagtcg gccgcgtcga
      661 tcaacccggg gtgggccagg atgtccggcc cggcctcgat ggccgcccgg ttggtgccgg
      721 gcaccaccgg ttcggccagg gtctcgccgt ggaccagcac caggcgcgcg cccagccgcc
      781 gggcggcatc caccagcccg gcgatcgaac cggccggcgc gtgggtcacc tcggcgcccg
      841 ggatgacccg gaaaccggcc gaccggcggt tgatctcggc ggccgccttc agcacctccg
      901 ggatcaccct ttccaggttg gaggcatcga catggtcggt gatggccagc cagcggtaac
      961 cacggcactc agcccgccgg gccagctcca tcggcaggta gagcccgtcg gaaagcagcg
     1021 aatgggtatg cagatcaacc agtttcatgc ttgctccccc ggccggcgtt gccccggcct
     1081 tcccggtgcg cacgctcatg ccttctccgg aaccaccttg aggccgcgca cctcgacgtc
     1141 caccttggcc acgcgcatcc cggtcatcgc ctcgaccgcc tccttgaccg ccttctggac
     1201 ctcccaggcc agctggggca catccttgcc gtagagcacc tcgacggcca gttcaaaaaa
     1261 aaggcattcc tcgcgcaccg tcaccttgac cgagtgctgg ggcgccgacc ggccgaagag
     1321 ctcggccaac ccgcccacga aaccgggaat gatgcgcgaa acgccggaga cctttccggc
     1381 ggctgcggcg gcgatcgccg ccagcacctc gtgttcaatc tttacgttgc ccaggttctc
     1441 tttggccata aagtcatcct cgcgtctccg ccgcccgttc gatatcggcg cggcaggccg
     1501 gccccgatcc gggacggcgg aaaattattg accggccgcc gattcgtcgc tctgctcctt
     1561 ttccagcatc gtctcgatca gcccgacata gtacttgccg ctcaggaaac cggcgtggtc
     1621 cagaagcttt cggtaaaggg gcacggtggt cttgaccccg tcgatctcga gttccatgag
     1681 ggcgcgccgc aaccgctcca gcgcctcgcg ccggtcgcgt ccgctcacga tcagcttgcc
     1741 gatcagcgaa tcgtagtagg gcggcacccg gtaaccctgg tagaggtggc tgtcgaaacg
     1801 gacgccgggt ccgccgggca ccaacagccg gttgaccgtg ccgggcgacg gccggaaatt
     1861 catggccggg tcc
//
LOCUS       DAOQYN010000285         1854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_74817_length_1854_cov_3.249583, whole genome shotgun sequence.
ACCESSION   DAOQYN010000285 DAOQYN010000000
VERSION     DAOQYN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74817_length_1854_cov_3.249583"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKN80_08895"
     CDS             complement(<1..237)
                     /locus_tag="PKN80_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNQ36163.1"
                     /translation="MRIEELDYPLPEAAIAQEPAAERDRARLLILDRASGRMAEAAFA
                     DLPDRLAAGSLLVLNDSRVRPARLRFTKASGGRLE"
     gene            complement(241..951)
                     /locus_tag="PKN80_08900"
     CDS             complement(241..951)
                     /locus_tag="PKN80_08900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNQ36164.1"
                     /translation="MEKLILAYHRVNDWSDDTLVVPPGEFRRQLTFLKARYRVVPLLE
                     LAEARPEPGGQRLAAISFDDGYRDNFRWAASILAELKLTATFFLTAGLIGTDRLLPRD
                     RAGADFEGNGLMTWTEVIRLQAQGFSFGSHGLSHLKLTGADPETARREIAESKQIIET
                     RLEREVNLFCYPYGAWNEEVARLVAGAGYRYAFVTPGGRQAGENDYTLKRVGVYRHTA
                     FWQFRLKTHPLYRKLRGR"
     gene            complement(979..>1854)
                     /locus_tag="PKN80_08905"
     CDS             complement(979..>1854)
                     /locus_tag="PKN80_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNQ36165.1"
                     /translation="VRHYSEPFGDSSAIPTFYLARETRKHVTVALSGDGGDENFAGYP
                     RYRQALELERLLPLLRWAAPLAARRGDALRRGWQRKLDWALGEAPRLTPAGRYGRWIT
                     VFPADQRERLYRGRLREQLARNALDRLEAVWARTGDRLDRMLDADFQLYLPDCLQVKM
                     DIASMAHGLEVRSPLLDQEVVELAASLPAGLKLRRGAGKYLFRREMRELLPPEILKRR
                     KMGFGIPLSPWFRRELKDYLAGHILDRRALGRGYFEPEAVRRLFEEHVSGRVDHTSRL
                     YLLLVLELWHRLYLD"
BASE COUNT          270 a          648 c          641 g          295 t
ORIGIN      
        1 ttccagccgg ccgccgctgg ccttggtgaa ccgcagccgg gccggccgga cgcggctgtc
       61 gttgagcacc aggaggcttc cggccgccag ccggtcgggc aggtcggcga aggccgcctc
      121 ggccatccgg ccgctggccc ggtccagtat cagcagccgg gcccggtccc gctcggccgc
      181 cggctcctgg gcgatggccg cctccggcag cgggtaatcg agttcttcga tgcgcattgg
      241 tcagcggccg cgcagcttcc ggtaaagggg atgcgtcttg agccggaact gccagaaagc
      301 ggtatggcgg taaaccccca cccttttcag cgtgtaatca ttctccccgg cctggcggcc
      361 gccgggggtc acgaaagcgt aacggtagcc ggccccggcc accagccggg ccacctcctc
      421 gttccaggcg ccgtagggat agcagaagag gttcacttcc cgttccagcc tcgtttcgat
      481 gatctgtttc gactcggcga tctcgcgccg ggccgtctcc gggtcggccc cggtcagttt
      541 caggtggctc aggccgtgcg atccgaagga gaaaccctgc gcctgcagcc ggattacttc
      601 cgtccaggtc atcaggccgt ttccttcgaa gtcggccccc gcccggtctc ggggcagcag
      661 ccggtcggtc ccgatcaggc cggcggtcag gaagaaggtg gccgtcagtt tcaactcggc
      721 caggatggac gccgcccagc ggaaattgtc ccggtacccg tcgtcgaagc tgatcgccgc
      781 cagccgctgg ccgcccggct ccggccgggc ttcggccagc tccaggagcg ggaccacccg
      841 gtagcgcgcc ttcaggaagg tcagctggcg ccggaattcg ccgggaggca cgaccagggt
      901 gtcgtcggac cagtcgttga cgcggtgata ggccaggatc agtttttcca tgggacggta
      961 tcggttgcgg cccggtcctc agtcgaggta aagccggtgc cagagctcca ggaccagcag
     1021 caggtagagc ctcgaggtgt ggtcaacccg ccccgagacg tgctcctcga agaggcggcg
     1081 caccgcttcc ggctcgaagt agccgcgccc gagcgcgcgg cggtccagga tgtggccggc
     1141 caggtaatcc ttgagttccc ggcggaacca gggtgagagc gggatgccga agcccatctt
     1201 ccgccgcttc agtatctccg gcggcagcag ttcccgcatt tctctgcgga agaggtactt
     1261 gccggcgccc cggcgcagtt tcagcccggc cggcaggctg gccgccagtt cgaccacctc
     1321 ctggtccagg agcgggctgc gcacctccag gccgtgggcc atcgaggcga tgtccatctt
     1381 cacctggagg cagtcgggca ggtagagctg aaaatcggcg tccagcatcc ggtccagccg
     1441 gtcgccggtg cgcgcccaga ccgcctccag gcggtccagg gcgttgcggg ccagctgttc
     1501 ccggagccgt ccccggtaaa ggcgttcccg ctggtcggcc gggaagacgg ttatccagcg
     1561 cccgtaacgg ccggccggcg tcaggcgggg cgcttcgccc agcgcccagt ccagctttcg
     1621 ttgccagccg cgccggagcg cgtcgccccg ccgggccgcc agcggcgccg cccagcgcag
     1681 caggggcagc agccgttcca gttccagcgc ctgccggtac cggggatacc cggcgaaatt
     1741 ctcgtcgccg ccgtccccgg agagggccac cgtcacgtgc ttgcgcgtct cccgggccag
     1801 atagaaggtc ggtatcgccg aactgtcgcc gaagggttcg ctgtagtggc gcac
//
LOCUS       DAOQYN010000286         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_75799_length_1839_cov_5.068386, whole genome shotgun sequence.
ACCESSION   DAOQYN010000286 DAOQYN010000000
VERSION     DAOQYN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75799_length_1839_cov_5.068386"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1839
                     /locus_tag="PKN80_08910"
                     /pseudo
     CDS             246..1839
                     /locus_tag="PKN80_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          351 a          591 c          645 g          252 t
ORIGIN      
        1 aggcggcggt ggccgagatc atccgggcga tcaggacggt ccggaacggc ggtaattccg
       61 ggtgaaggcg gggcggggcc ggtttgacgg cggatccggg agcggtcggt ttcggggcgc
      121 gccgatggcg cgccgctgaa actgacggcc cggtccggga atctataata tagagagcac
      181 cagtcaaacc ggcacaagga ggatcaagag acatggacgg acaacatcag cccggaacgg
      241 gcggcccgga gaccgattcc cgcccggccc tggaacgttt cacccgcgac ctgaccgaac
      301 tggcccggca ggaccggctt gacccggtgg tcggccggga cgaggagatc cggcgggtca
      361 tccaggttct ctcccgtcgc accaagaaca acccggtcct gatcggcgag gccggggtcg
      421 gcaagacggc catcgtcgaa ggcctggccc agcgggtgaa ctccggggac gtcccggaaa
      481 gcctcaagaa gcagcgcgtt ctttccctgg acatcggcgc cctggtcgcc ggcgcggtct
      541 accgcggcca gttcgaggat cgggtcaagt cgcttttgaa ggaactggag aagcagccgg
      601 gccaggtgat ccttttcatc gacgagctcc acaccctggt gggcgccggg gccgccgaag
      661 gcgcggtgga cgcctccaac ctgctcaagc cggccctggc ccggggcgag ttgcgcacca
      721 tcggggccac caccctgaac gagtaccgca agtatataga gaaggaccgc gccctcgaac
      781 ggcgcttcca gccggtttac gtccgggagc cgaacgtcgc cgacaccatc tccattctgc
      841 gcggcctgcg cgagaagtac gaggtgcacc acggcgtcaa gatcaaggat gccgccctgg
      901 tggccgccgc cgccctttcc aaccgctaca tcaccgaccg tttcctgccc gacaaggcga
      961 tcgacctgat cgacgaggcc gccagccggt tgcgcatgga gatcgacagc ctgcccggcc
     1021 ccctggacga ggtggaacgg cggatccgtc agctggagat cgaacgccag gccctgaaaa
     1081 aggagccgga aggcgtttcg cgggaacgcc tggccgccct ggagaaggac ctggccaccc
     1141 tgcaggggga acggaaagaa ctccgcggcc agtgggagaa ggagaaggcc cgcatccaga
     1201 agatccagca gctccaggcc gggatcgaga agttccggct ggaggccgag cgggctgaac
     1261 gggaaggaaa tctgggccgg gcggccgaac tccgttacgg ccggctgccg gaggcgaaca
     1321 aggagctggc ggcggcctcc gccgggctgg ccgagattca gaaggaacgg cgcctgctca
     1381 aggagagcat cgacgaggag gagatcgctg agatcgtctc ccgctggacc ggcatcccgc
     1441 tggcgcggat gatggagacc gagaccgaga aactcctgca gatggaggac cgcatccacc
     1501 ggcgcctggt tggccaggag gaggcggtgg cggccgtttc caatgccatc cgccgcagcc
     1561 gttccggcat ctccgatccg aaccggccgc tgggctcctt catcttcctc gggccgaccg
     1621 gcgtcggcaa gaccgagctg gcccgcagcc tggccgagtt cctctttgac gacgaaaagg
     1681 cgctggtccg catcgacatg tccgagtaca tggagcgcca cgcggtaagc cgcctggtcg
     1741 gcgcgccgcc cggttacgtg ggcttcgagg agggcggcca gctgaccgag attatcaggc
     1801 gccggcctta cgcggtcatc ctgctggacg aaatcgaga
//
LOCUS       DAOQYN010000287         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_77041_length_1821_cov_3.712344, whole genome shotgun sequence.
ACCESSION   DAOQYN010000287 DAOQYN010000000
VERSION     DAOQYN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77041_length_1821_cov_3.712344"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKN80_08915"
     CDS             complement(<1..654)
                     /locus_tag="PKN80_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ36166.1"
                     /translation="MKAKRFWLRLLCQAVSLGLFFWLAWATRYPLRLKGWGSLYFHLD
                     PFLGLGASLAALRPVLQFWPALLLLALTFIWGRFFCWYLCPMGAAMDLSYWLLGRRFW
                     PLKLRVAGRPAVQTGFLLVFLLLALFGFSGVGQRFDPLAISLGAMLSRPGLLVGLLIL
                     IISLVFPRFWCFTICPLGGLLEWTRRLGRRLRRRDPAFAGGLTRRQFLGIMTAGVAAA
                     "
     gene            complement(654..1556)
                     /locus_tag="PKN80_08920"
     CDS             complement(654..1556)
                     /locus_tag="PKN80_08920"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ36167.1"
                     /translation="MNRRDFLKLSSVSLAGAFLMGLFGRRKAWSAGPVPDLVEVTGGT
                     PRERVAAAFKALGGIKKFVPTGSTVVVKPNIAWARNPEQGADTDPQIVAEVVTACRQA
                     GAKRVVVMDHTCDKAQLTYPQSGIEAAARAAGAEVLHADQKDAYREVAIPKGKALTSA
                     LVLKEVLDCDVLINLPVVKVHGSSRVTLGMKNWMGVVWDRGALHRSDLHQAIVDISTV
                     VKPRLVLLDATRILTSRGPKGPGDLTVLNQLVAGTDPVAADAYGARILGFNPQEIGHI
                     RLAGEAGLGKSNLDALKVQKIKLG"
BASE COUNT          375 a          588 c          585 g          273 t
ORIGIN      
        1 ggcggccgcc accccggcgg tcatgatccc gaggaactgg cgccgggtca ggccgccggc
       61 gaaggcgggg tcgcgccggc gcagccggcg gccgaggcgg cgcgtccatt ccagcaggcc
      121 gcccagcggg cagatggtga agcaccagaa acggggaaag accaggctga taatcagtat
      181 cagcaggccg accagcagac cgggccggga gagcatcgcg cccagggaga tggccagcgg
      241 atcgaagcgc tgccccactc cgctgaaccc gaagagggcc agcagtagaa agaccaggag
      301 gaagccggtc tggaccgccg gccgtccggc cacccgcagc ttcagcggcc agaagcgtcg
      361 gccgagcagc cagtaagaaa gatccatggc cgcccccatc gggcagagat accagcagaa
      421 gaagcggccc cagataaagg tcagcgccag caggagcagg gccggccaga actggagaac
      481 cgggcgcagg gcggccagcg aggcgcccag gcccaggaag gggtcgaggt gaaagtaaag
      541 gctgccccag cccttaagtc gcagggggta acgggtcgcc caggccagcc agaagaagag
      601 ccccagcgaa accgcctggc agagcagccg cagccagaat cttttcgcct tcatcaaccc
      661 agcttgatct tctgaacctt aagcgcgtca aggttggact tgcccagccc ggcttcgccg
      721 gccagccgga tatggccgat ctcctgcggg ttgaagccca ggatgcgggc gccgtaggca
      781 tcggcggcca ccgggtcggt gccggccacc agctggttga gcacggtcag gtcaccgggc
      841 cccttcggcc cccgggaggt gagaatccgg gtggcgtcca ggaggaccag cctgggcttg
      901 accacggtcg agatatccac aatcgcctgg tgcaggtcgg aacggtgcag ggcgccgcgg
      961 tcccagacca cgcccatcca gttcttcatg cccagggtca cccggctgct gccgtgcacc
     1021 ttgaccaccg gcaggttgat cagcacgtcg cagtccaaga cttccttgag aacgagggcc
     1081 gaggtgagcg ccttgccctt gggaatggcc acctcgcggt aggcgtcctt ctggtcggcg
     1141 tgcagaacct ccgctccggc cgcgcgggcg gccgcctcga taccgctctg cgggtaggtg
     1201 agctgggcct tgtcgcaggt gtggtccatg accaccaccc gcttggcgcc ggcctggcgg
     1261 caggcggtga ccacttcggc cacgatctgc ggatcggtat cggccccctg ctccgggttc
     1321 cgggcccagg cgatgttcgg ctttaccacc accgtgctgc cggtcggcac gaacttcttg
     1381 attccgccca gggccttgaa agcggccgcc acccgttcac ggggcgtgcc gccggtcacc
     1441 tcgaccaggt ccggaaccgg tccggcgctc caggccttac ggcgtccgaa gaggcccatg
     1501 agaaaggccc cggcaagcga aaccgaagat aatttcaaaa aatcgcgtcg attcatgccg
     1561 tcctcccttt ccagtcagca actttaatcg atcagtcaac ccggccgttt cgtcaaaaaa
     1621 agaagaattc agaaaaatta ttattacctt gctcatatta tacttgcccc ttcccgcctt
     1681 gtaaaggggc cgatcgaacc ggaacggcgc cggagacggt caggaagaaa caagaaaaat
     1741 aaaacgctgt ttttcaatac tatatgttgg ttaatttaca cctttttata ggcaggcgcc
     1801 gacggccgga aaggaccgga c
//
LOCUS       DAOQYN010000288         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_77339_length_1817_cov_4.410897, whole genome shotgun sequence.
ACCESSION   DAOQYN010000288 DAOQYN010000000
VERSION     DAOQYN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77339_length_1817_cov_4.410897"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PKN80_08925"
     CDS             complement(<1..1012)
                     /locus_tag="PKN80_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36168.1"
                     /translation="MAVVAELHLESCPRGSLFDENRLDWSKEKKHLPGWRNALSAGPW
                     WKLRPSLFLDTDREGPYLEHIIGHEVRLVAGQRLMSDYTVRGRFRMLTENYRPIHTDD
                     LWAIKGRVGPVVRMMDHRRYYLLCLEGLERIVLYRREDEETYQLAALRKKLSPLEYYE
                     LELTARGTRLTARLNGRKLFEVEDEHYPYGWPGVRFHAVSRAQGLQVLAGEADQRVNE
                     RRLRAWRDREGELRAETPQPKLWKEINIKKYAGMGAVVTRVAGEPRLLFIGKEELILA
                     DLKLKERWHRKLAVNNPCLGPLDGPETARLAALSGDSILVLDLADGRELRRAPLPPAD
                     PYI"
     gene            complement(1236..>1817)
                     /locus_tag="PKN80_08930"
     CDS             complement(1236..>1817)
                     /locus_tag="PKN80_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36169.1"
                     /translation="AREEWFALHGIHFEPGERWKKTDRVLAFNCDLVDDPELNRVVYD
                     TLVAWGVKLPFSRDEIHKIYANLDRSIEVNTDLGRMKVDRDDFQAWLGQNHGEQTQTS
                     LMSVSTDADQVQVLAVPFDTGSFKTARQLVLWSRTDANVKLTADFDREPEIWAVNWTG
                     YRKQKVSPLAWDKKSVTVRFTHDLDVHYYEILR"
BASE COUNT          303 a          565 c          582 g          367 t
ORIGIN      
        1 ggatgtaggg gtcggccggc ggcagcggcg cccggcgcag ttcccggccg tcggccaggt
       61 ccaggaccag gatggagtca ccgctgaggg cggccagccg ggccgtctcc gggccgtcca
      121 gcggaccgag acaggggtta ttgacggcca gtttccggtg ccagcgttcc ttcagtttca
      181 ggtccgccag gatcagctcc tctttgccta taaacagcag ccggggctct ccggccaccc
      241 gggttaccac ggcccccatc ccggcgtact tcttgatatt tatctccttc cagagtttcg
      301 gctggggggt ttcggcgcgc agttcccctt cccggtcccg ccaggcccgc aaacgccgct
      361 cattcacccg ttggtcggcc tcgccggcca gtacctgcag cccctgggcc cggctgaccg
      421 cgtggaagcg cacgcccggc cagccgtacg gataatgttc gtcctccacc tcgaagagct
      481 tccggccgtt gagtctggcg gtcagccggg tgccccgggc ggtcagttcc agttcgtaat
      541 actcaagggg ggagagtttc ttgcgcaggg cggccagctg gtaggtctcc tcgtcctcgc
      601 gccggtagag gacgatccgt tccaggccct ccaggcagag caggtagtag cgccggtggt
      661 ccatcatccg gacgaccggc ccgacccgcc ccttgatcgc ccagaggtcg tcggtgtgaa
      721 tcggccggta gttttcggtg agcatccgga agcggccccg gaccgtgtag tcggacatca
      781 gccgctggcc ggccaccagc cggacctcgt ggccgataat gtgttccagg taggggcctt
      841 cgcggtcggt gtccaggaag agggagggac gcagtttcca ccaggggccg gcgctcaggg
      901 cgttgcgcca gccgggcagg tgcttcttct ccttggacca gtcgagccga ttttcatcaa
      961 agagcgaacc gcgcggacag ctttccaggt gcagctctgc gactacggcc atcttcacat
     1021 cctccttcaa ggttttggcg gttgaaaaac aggcgggacg attgacggga atttaaagtt
     1081 ttattataag gataaggggg gtaaaagtca acctttgcct gcctcgccct gattgtatat
     1141 caggcgacga tccctagggc ggttctcaaa agaggcgggc ggaggtttaa agccgccgcc
     1201 cgcctcgatt cctagatcag tttgccggtt tggcgtcagc gcagaatctc gtagtaatgc
     1261 acatccaggt cgtgggtgaa gcgcaccgtg accgattttt tatcccaggc cagcggggag
     1321 accttttgct tccggtagcc ggtccagttg accgcccaga tctccggttc acggtcgaaa
     1381 tcggcggtca gcttgacgtt ggcgtcggtc cggctccaga ggaccagctg gcgggcggtt
     1441 ttgaagctgc cggtgtcaaa cggcaccgcc agcacctgga cctggtcggc gtcggttgaa
     1501 acggacatca gcgaggtttg ggtctgctca ccatggttct ggcccagcca ggcctggaaa
     1561 tcgtcccggt ccaccttcat ccggcccagg tcggtattga cctcgatgga ccggtccagg
     1621 ttggcataga tcttgtgaat ctcgtcccgg ctgaagggga gttttacgcc ccacgcgacc
     1681 agggtgtcat aaacaacccg gttcaattcc gggtcatcga ccaggtcgca gttgaaggcc
     1741 aggacccggt cggtcttctt ccagcgttcg cccggttcga aatggatgcc gtgcagggcg
     1801 aaccactcct cgcgggc
//
LOCUS       DAOQYN010000289         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_79081_length_1792_cov_3.582038, whole genome shotgun sequence.
ACCESSION   DAOQYN010000289 DAOQYN010000000
VERSION     DAOQYN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79081_length_1792_cov_3.582038"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1620
                     /locus_tag="PKN80_08935"
     CDS             <1..1620
                     /locus_tag="PKN80_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36170.1"
                     /translation="PLQEYTFSVLRRPPERMAGSFGAYITVAPEPIEIMGRAGIRRTV
                     TLSSSNNLLQSWRDIEPEPGKFVWVDRRVAHALANGVAITANLHIGSGGDEVPKWARD
                     PSDPADVISCTGSRMAQDKKFTFSKRAWERFIEAMVGHYKESIQEWLIMDEPYYVFKA
                     EEYAELLKSAYRAAKWANPNCRVLAHGGYYAPWLPALEKAGAVPFFDAISDYARDPEQ
                     GQRLRDFARKHGKTVVNVEYGGHSSGYRSIETPEDTNDRRIPVYQSNAQSVLTGALRA
                     MGWSAAEGFRRYDARFPGGDFTQLDRYMSFFEYDGSLKPAAVAYATAGRLLDGFRGAG
                     ALELHNRLEAFLFEKEGRFLLAFWARENDLFQTQFLLPPEVSGFDLMGNPLEAGRPLA
                     VSGSPNYLAGPDSRREETLNLLRNLLVRELVSLEAETRLDEASGGYRLRVTIANLDPG
                     RPIQNGVLTLSGSGSVYGSATLLRESWDRVRLLPEVKPGGRLTIELDLNAYRDEIRTA
                     RPATLELFFNGASRAWKFPDIFKAGTPAAAP"
BASE COUNT          312 a          620 c          588 g          272 t
ORIGIN      
        1 ccgctgcagg agtacacctt ctcggtgctg cggcggccgc cggagcggat ggccggcagc
       61 ttcggcgcct atatcaccgt cgccccggag ccgatcgaga tcatgggccg ggccggtatc
      121 cggcgcacgg tcaccctcag ctcatccaac aacctgcttc agagctggcg ggatatcgaa
      181 ccggagccgg ggaagttcgt ctgggttgac cggcgggtgg cccacgccct ggccaacggc
      241 gtggccatca ccgccaatct gcacatcggc agcggcggcg acgaggtccc gaagtgggcg
      301 cgcgatccgt ctgacccggc cgacgtcatc tcctgcaccg gaagccggat ggcgcaggac
      361 aagaagttca ccttctcgaa gcgggcttgg gaacgcttca tcgaggcgat ggtcggccac
      421 tacaaggaga gcatccagga gtggctgatc atggatgagc cctactatgt cttcaaggcc
      481 gaagagtacg ccgaactgct caagtccgcc taccgggcgg ccaagtgggc caaccccaac
      541 tgccgggtgc tggcccacgg cggctactat gccccctggc tgccggccct cgagaaggcc
      601 ggcgcggttc ctttcttcga cgccatctcc gactacgccc gcgacccgga gcaggggcag
      661 cggctgcgcg atttcgcccg gaaacacggc aaaacggtcg tcaatgtcga gtacggcggc
      721 cactccagcg gctaccggag catcgaaacg ccggaggata ccaacgaccg ccggatcccg
      781 gtctaccagt ccaacgccca gtcggtgttg accggggcgc tccgggccat gggctggtcg
      841 gccgccgagg gcttccggcg ctacgacgcc cgcttcccgg gcggcgactt cacccagctg
      901 gaccgttaca tgagcttctt cgaatacgac ggaagcctga aaccggccgc ggtcgcctac
      961 gcgacggccg gccgcctcct ggacggcttc cggggcgccg gcgcgctgga actgcacaac
     1021 cgactggagg ccttcctctt cgaaaaagag ggccgcttcc tgctggcctt ctgggcccgc
     1081 gagaacgacc tcttccagac gcaattcctc ctgcccccgg aggtgagcgg cttcgacctc
     1141 atgggcaacc cgctggaggc cggccggccg ctggccgtct cgggctcccc caactacctg
     1201 gccggcccgg attcgcgccg ggaggagacc ctcaacctgc tgagaaacct gctggtccgg
     1261 gagctggtca gcctcgaggc cgagacccgg ctggatgagg cgtccggcgg ctaccgcctc
     1321 cgggtgacga tcgccaacct cgaccccggc cggcccattc agaacggggt cctcaccctc
     1381 tcgggcagcg gctccgtcta cggaagcgcc accctgctca gggaatcctg ggaccgggtg
     1441 cgcctcctgc ccgaggtgaa accgggcggc cggctgacca tcgagctgga tctcaacgcc
     1501 taccgggacg aaatccggac cgcccggccg gccaccctgg agctcttctt caacggcgcc
     1561 agccgcgcct ggaaattccc ggatattttc aaggccggga cgccggcggc ggccccctga
     1621 cgcccgtctc cgtcttgaaa aaaaaagagc gtggcattaa aattgaagtc cgcggacggg
     1681 gggcgccggc taaacggccg ccgggcggat accctctaaa attaggacgg agctgaagat
     1741 gaagaaggcc tggaaacggg ggttgttgat tttcggggcg gcggtcctgg cg
//
LOCUS       DAOQYN010000290         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_79606_length_1785_cov_3.036994, whole genome shotgun sequence.
ACCESSION   DAOQYN010000290 DAOQYN010000000
VERSION     DAOQYN010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79606_length_1785_cov_3.036994"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PKN80_08940"
     CDS             complement(<1..645)
                     /locus_tag="PKN80_08940"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNQ36171.1"
                     /translation="MNYFPTEFSRLPEFETARRRLQGAAGQVKLTGLSGSARSLAAAA
                     LFEAPPGFRRALLVTPGVESALRICADLQTFLDQDRVRRFPFAEVLPDEDLRPPAEIE
                     GQRLLTLNRLLEEPGPLLVVAPVQALMLPVPEPEGLKRNTVRLIVGREIDRPALIEFL
                     DRTGYERCDLVAEPGNFAVRGGIIDLFPLTRPEPIRIELSGRTVISLRRFNPQSQ"
     gene            complement(669..>1785)
                     /gene="glyA"
                     /locus_tag="PKN80_08945"
     CDS             complement(669..>1785)
                     /gene="glyA"
                     /locus_tag="PKN80_08945"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNQ36172.1"
                     /translation="AATGSVLTNKYAEGYPGRRYYGGCRFVDEVERLAIERARELFGV
                     EYANVQPHSGTQANMAVILSLLQPGDRIMGMSLDSGGHLSHGARANFSGRLFENHPYT
                     VDRKSERLDYDAIARLAAEVKPKLIICGASAYPRIIDFARFREIADGVGACLMADIAH
                     IAGLVIAGLHPSPVGQAHFVTSTTHKTMRGPRGGLILSDRKHGQMLDRMIFPGIQGGP
                     LMHVIAAKAVALHEALQPAFKDYQRQIVRNCRVLAESLLEKGFRLVTGGTDNHLVLID
                     LTNRRLTGRQAEDRLEATGISCNKNMIPFDPLDPKTTSGLRLGTPAITSRGMKEPEMR
                     LIAGLIDRVLREPAAVKPETIRREVEDLCRKFPLPVS"
BASE COUNT          280 a          556 c          646 g          303 t
ORIGIN      
        1 ctggctctgc gggttgaagc gccgcagcga gatgacggtt cggccggaga gctcgatgcg
       61 gatcggttcc ggccgggtca ggggaaagag gtcaatgatg ccgccccgga ccgcgaagtt
      121 gcccggttca gcgaccaggt cgcagcgttc gtagccggtc cggtccagga actcgatcag
      181 cgccggacgg tcgatctccc ggccgacgat cagccggacg gtgttccgct tgaggccctc
      241 cggttccgga accggcagca tcagggcctg gaccggcgcc acgaccagca gcggccccgg
      301 ttcctccagg agccggttca aggtcagcag ccgctgtccc tcgatttcgg ccggcggccg
      361 caggtcctcg tcgggcagca cctcggcaaa gggaaagcgc cggacccggt cctggtccag
      421 gaaggtctgg aggtcggcgc aaatccgcag ggcgctctcc acgccggggg ttaccaggag
      481 ggcccgccgg aacccgggcg gggcttcgaa gagggcggcg gcggccaggc tgcgggccga
      541 tccggagagg ccggtgagct tgacctgtcc ggccgcgccc tgaaggcggc ggcgggccgt
      601 ttcaaattcc ggcaggcggg agaattcggt cgggaagtaa ttcacggccg gcaagtccga
      661 cccgtctttc aggaaaccgg gagcgggaac ttccggcaga ggtcttcgac ctcccgccgg
      721 attgtttcag gtttgacggc ggccggttcc cggagcacgc ggtcaatcag gccggcgatg
      781 aggcgcatct ccggttcttt catgccccgg ctggtgatcg ccggggtccc gaggcgcagg
      841 ccgctggtgg tcttggggtc cagcgggtcg aaggggatca tgttcttgtt gcaggagatc
      901 ccggtcgcct cgagccggtc ttcagcctgg cgtccggtca ggcggcgatt ggtcaggtcg
      961 atcaggacca ggtgattgtc ggttccgccg gtgaccagcc ggaagccctt ttccagcagg
     1021 gattcggcca ggacccggca gttgcggacg atctggcgct ggtaatcctt gaaggcgggc
     1081 tggagcgcct cgtgcagcgc gaccgccttg gccgcgatca cgtgcatcag cggaccgccc
     1141 tggatgccgg ggaagatcat ccggtccagc atctggccgt gtttccggtc cgacaggatc
     1201 aggccgcccc gggggccgcg catggtcttg tgggtggtgc tggtgacgaa atgggcctgg
     1261 ccgaccgggc tgggatggag gccggcgatc accagtccgg cgatatgggc gatgtcggcc
     1321 atcagacagg cgcccacccc gtcggcaatc tcccggaacc gggcgaaatc tatgatccgg
     1381 ggataggcgc tggccccgca gatgatcagt ttcggtttta cctcggccgc cagtcgggcg
     1441 atggcgtcgt aatcgagccg ctcgcttttc cggtcaaccg tgtagggatg attttcaaag
     1501 aggcgtccgg agaagttggc ccgggcgccg tggctcaggt ggccgccgga atcaaggctc
     1561 atgcccatga tccggtcgcc gggctgcaaa agcgaaagaa taacggccat gttggcctgg
     1621 gtgcccgagt gaggctggac attggcgtac tcgactccga agagttcccg ggcccgttcg
     1681 atcgccaggc gttccacctc gtccacgaag cggcagcccc cgtaataccg gcggccggga
     1741 tagccctcgg cgtacttgtt ggtcagcacc gatccggtgg ccgcc
//
LOCUS       DAOQYN010000291         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_82098_length_1752_cov_2.818503, whole genome shotgun sequence.
ACCESSION   DAOQYN010000291 DAOQYN010000000
VERSION     DAOQYN010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82098_length_1752_cov_2.818503"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..725)
                     /gene="dps"
                     /locus_tag="PKN80_08950"
     CDS             complement(168..725)
                     /gene="dps"
                     /locus_tag="PKN80_08950"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HNQ36173.1"
                     /translation="MELVSRKLVEKAGINVEKLLKLLVSNAAAELTTFYYYTALRINL
                     IGLEGEGLKEIAEDARIEDRNHFEALVPRIYELGGKLPDDMKVFHDLSACPPARLPKD
                     PTDVKAIVQVLLKAEQCAVTGYTNICNLTCGKDHRTYDLALAILNEEIEHEAWFAEFL
                     AKGPSGHFRRRQVGESPFVSKFLHP"
     gene            complement(891..1358)
                     /locus_tag="PKN80_08955"
     CDS             complement(891..1358)
                     /locus_tag="PKN80_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNQ36174.1"
                     /translation="MNLQNKKAALEKYLADFDEFCRGEGIKVTHQRREVYREVIQTTE
                     HPDVETVYGRVRQRIPTISLDTVYRILRLFEARNLVFRTGEPGKRARFDANPKPHHHF
                     FCVKCGALLDIYNEEFNQIRVPQDLDNVGRIENVQVEMRGTCARCCKEKTTVE"
     gene            1581..>1752
                     /locus_tag="PKN80_08960"
     CDS             1581..>1752
                     /locus_tag="PKN80_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ36175.1"
                     /translation="MKEDWKYDLVVVGGGVAGVCGALAARRHGLKTALIQDRPVLGGN
                     ASSEIRVNISGAA"
BASE COUNT          320 a          480 c          529 g          423 t
ORIGIN      
        1 cgcacctgaa tggggttcag gtggtcgctg gttcgaatcc agtcgccccg accagggaat
       61 tgaaaaaggc cgttcggcgg aaagtcgaac ggcctttttt tgtggcgtgt ttttccgggg
      121 aggcggaccg attaaaagat ccgcctcccc gtttcggttg ccgggcttta cggatgcagg
      181 aacttcgaga cgaagggcga ttcgcctacc tgccgccggc ggaagtggcc cgatgggccc
      241 ttggccagga actcggcgaa ccaggcctcg tgttcgatct cctcgttcag gatcgccagg
      301 gccaggtcgt aggtccggtg gtccttgccg caggtcaggt tgcagatgtt ggtgtagccg
      361 gtcacggcgc actgctcggc cttgagcagg acctggacga tggccttgac gtcggtgggg
      421 tccttgggca gccgggccgg cgggcaggcc gaaaggtcgt ggaagacctt catgtcatcc
      481 ggcagcttgc cgccaagctc atagatcctg ggtaccaggg cctcgaagtg gtttcggtcc
      541 tcgatccggg catcctcggc gatctccttc aatccctcgc cctccaggcc gatcaggttg
      601 atgcgcagcg cggtgtagta gtagaaagtg gtcaactcgg ccgcggcgtt gctgaccagc
      661 agcttcagga gcttctccac gttgatgccg gccttctcca ccagcttgcg ggataccaat
      721 tccatgtcgc ctcctcgttt atttgtgacc tcgtttcgga tctgaaaagt ttgggccttg
      781 accggccccg gacattatgg cgggcatcac ctcctttagc cttcttttat agttttattc
      841 cgatttagta aagattatta tggttgacta aaaaaaagat cctcacttct tcattcaacg
      901 gttgttttct ctttgcagca cctggcgcag gtgccccgca tttcgacctg gacattttcg
      961 attcgcccga cattgtccag gtcctgggga acccggatct ggttgaattc ttcgttgtag
     1021 atgtcgagga gcgccccgca cttcacgcag aagaagtgat gatgtggttt cggattggcg
     1081 tcgaaacggg cccgcttgcc gggttctccg gttcgaaaga ccaggttgcg cgcctcaaag
     1141 aggcggagga tgcggtagac ggtgtcgagc gaaatcgtgg gtatgcgctg gcgcacccgg
     1201 ccatagacgg tttcgacatc cgggtgctcg gtcgtctgga tcacctctcg gtagacctcc
     1261 cgccgctggt gggtcacctt gatgccttcc ccccggcaga attcatcgaa atcggccagg
     1321 tacttttcaa gcgctgcttt tttattctgc agattcatat cagtagacat tattaattaa
     1381 taatattgca aatctgtcca tctgtcaaat atcggattca aagcgcaacc ggaagcttca
     1441 aggtttttcc tcgggtttac atggttgaaa gttcttcgga tggttgacaa tttcggcgcc
     1501 gccggcttaa ataagattac cgccgtccgg cccggatcaa tccgggtcgg acggcgcttt
     1561 gcacacttat cggagttttc atgaaagaag attggaagta tgacctggtg gtggtcggcg
     1621 gcggggtggc cggggtctgc ggggccctgg cggcccggcg ccacgggctg aagacggcct
     1681 taatccagga ccggccggtc ctcggcggca acgccagctc ggagatcagg gtcaatatct
     1741 ccggcgcggc cg
//
LOCUS       DAOQYN010000292         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_82531_length_1746_cov_3.884092, whole genome shotgun sequence.
ACCESSION   DAOQYN010000292 DAOQYN010000000
VERSION     DAOQYN010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82531_length_1746_cov_3.884092"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PKN80_08965"
     CDS             <1..610
                     /locus_tag="PKN80_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36176.1"
                     /translation="TGLEFPDESSAAERIRMARAAGAEVIIGHPYWCGHQLPHLMPLQ
                     GAAAVEVFNTTCTKRGKGYSSVQWDDLLDSGWWLPAVAVDDTHADWDLFKGWTMFKLE
                     SLTPAAFLEALRTGSFYASCGPVIEDFSLSGDRAKVRLRSSPVTEIHLVAQRSCGRSF
                     YAQPGETITEAEMELGPGVRYLRGEVVDREGRRAWTNPFWLV"
     gene            659..>1746
                     /locus_tag="PKN80_08970"
     CDS             659..>1746
                     /locus_tag="PKN80_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HNQ36177.1"
                     /translation="MSKVLIIGAGGVGGVVAHKCAQVPEVFSEIMLASRTLEKCEKIK
                     AQLPRPIETARVDADRPAEVVALMKRFRPDLLINVALPYQDLSLMDACLEAGVDYLDT
                     ANYEPPEEAKFSYTWQWAYRDRFRAKGLMALLGSGFDPGVTNVFCAYAQKHYFDEIHA
                     VDIMDCNAGSHGHPFATNFNPEINIREITQPGRYWEGGRWIETGPLEVHRSFDFPQVG
                     PREMYLLYHEEMESLVNNIKGLKRIRFWMTFGESYLTHLRVLENVGLTRIDPVLYEGH
                     PVVPLKFLKSLLPDPASLAENYTGKTNIGCLFEGIKDGKARRIYIYNVCDHAACYREV
                     KAQAISYTTGVPAMIGGLLMVTGAWRGQG"
BASE COUNT          315 a          580 c          560 g          291 t
ORIGIN      
        1 gaccggtctc gagtttcccg acgagagcag cgcggccgag cgcatccgga tggcccgggc
       61 ggccggcgcc gaggtgatta tcggccaccc ctactggtgc ggccatcagc ttccgcacct
      121 gatgccgctg cagggcgcgg cggccgtcga ggtattcaac accacctgta ccaagcgggg
      181 caaggggtac agctcggtcc agtgggacga cctgctcgat tccggctggt ggctgccggc
      241 cgtggccgtt gacgacaccc acgccgattg ggacctcttc aagggctgga cgatgttcaa
      301 gctggaatcg cttaccccgg ccgccttcct ggaggccctg cgcaccgggt ccttttacgc
      361 ctcctgcggc ccggtgatcg aggatttctc tctttccggt gaccgggcga aggtccggct
      421 gcgctcttcg ccggtaaccg agatacacct cgtggcccag cgcagctgcg gccggagttt
      481 ttacgcccag cccggggaga cgatcaccga ggccgaaatg gaactgggcc ccggcgtgcg
      541 ctatctgcgg ggcgaggtgg tcgaccggga ggggcgccgc gcctggacca atcccttctg
      601 gctggtctga gccggcggtc agccggacat tttcccaacg gagaacggag cgaaaagaat
      661 gtcgaaagta ctgataatcg gggccggcgg ggtgggcggc gtggttgccc acaagtgcgc
      721 ccaggtgccc gaggtcttca gcgaaatcat gctggccagc cggaccctcg agaagtgcga
      781 gaagatcaag gcgcagctgc cgcggccgat cgaaacggcc cgggtggacg ccgaccggcc
      841 ggccgaggta gtggccctga tgaaacggtt ccggcccgac ctgctcatca acgtggcgct
      901 tccctaccag gacctttccc tgatggatgc ctgcctggag gccggggtgg attacctgga
      961 caccgccaac tacgaaccgc cggaggaggc gaagttctcc tacacctggc agtgggccta
     1021 ccgcgaccgt ttccgggcga agggcttgat ggccctgctg ggcagcggat tcgacccggg
     1081 cgtcaccaac gtcttctgcg cctacgccca gaagcattac ttcgacgaga tccacgccgt
     1141 ggatatcatg gactgcaacg ccggctcgca cggccacccc ttcgccacca acttcaaccc
     1201 agagatcaac atccgtgaaa tcacccagcc cggccgttac tgggaaggcg gccgctggat
     1261 cgagaccggc cccctggagg ttcaccggag cttcgatttc ccccaggtcg gcccccgcga
     1321 gatgtacctg ctctaccacg aggagatgga gtccctggtc aacaacatca agggcctgaa
     1381 acgaatccgc ttctggatga ccttcggcga gagctacctg acccacctgc gggtgctgga
     1441 gaatgtcggc ctgacccgga tcgacccggt gctctacgaa gggcacccgg tggtccccct
     1501 caagttcctg aaatccctgc tgccggaccc ggccagcctg gccgagaatt ataccggcaa
     1561 gaccaacatc ggctgcctct tcgagggaat caaggacgga aaggcacgcc ggatctacat
     1621 ctacaatgtc tgcgatcacg ccgcctgtta ccgggaggtc aaggcccagg ccatttccta
     1681 caccaccggc gtgccggcca tgatcggcgg gctgctcatg gtcaccggcg cctggcgcgg
     1741 ccaggg
//
LOCUS       DAOQYN010000293         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_82752_length_1743_cov_4.517180, whole genome shotgun sequence.
ACCESSION   DAOQYN010000293 DAOQYN010000000
VERSION     DAOQYN010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82752_length_1743_cov_4.517180"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PKN80_08975"
     CDS             <1..483
                     /locus_tag="PKN80_08975"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNQ36178.1"
                     /translation="FNVYGPRARTSGTYGAVFGVFLAQKLNGRPFTVVGDGSQTRDFV
                     FVTDVARAFVAAAESEAAGQVFNVGSGRPQSINRIVELLGGEKIHIPRRPGEPDCTHA
                     DISRITAELGWRPEVSLEAGVKIMLENIDYWRQAPLWTPEKIAEATRDWFTHLDRKEP
                     "
     gene            480..>1743
                     /locus_tag="PKN80_08980"
     CDS             480..>1743
                     /locus_tag="PKN80_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNQ36179.1"
                     /translation="MTESPKLKELDQLARTLAALKGKGRTIVHCHGVFDLLHPGHIKH
                     FQAARKLGDVLVVTVTPDEYVNKGPGRPVFNQRLRLESIAALECVDYVALNRWPSAEE
                     TIRRLKPDIYVKGSDYAESNKDLTGKINDERRAVESVGGRIHFTDEVTFSSTRLVNAF
                     FSVYPEEAGRFLEGFRRRHSAESVIGRLQELAGTRALVIGDAIIDQYAYCQPMGKAGK
                     ESVLVARHLSQEEFAGGALACANHLAGFCREVTLVTGLGRGNSREKFIRERLRPNIKP
                     VFFSRSDAPTVVKRRFVEPPFLSKLFEICYLNDANLPAEVEKRLAAFLERELPRYDLV
                     VAADFGHGFITPATVGLLGRRAPFLALNTQTNSANSGYNLVTRYRRADYICVDEPEMR
                     LANQDRFGRLEKIIQATGKQLDCRRTIVT"
BASE COUNT          313 a          645 c          536 g          249 t
ORIGIN      
        1 ttcaacgtct acggcccccg ggcccgcacc tccggcacct acggggcggt cttcggggtc
       61 ttcctggccc agaagctcaa cggccgcccc ttcaccgtgg tcggtgacgg cagccagacc
      121 cgggatttcg tcttcgtcac cgacgtggcc cgggccttcg tggccgccgc cgaatccgaa
      181 gccgccggcc aggtcttcaa cgtgggcagc ggccgccccc agagcatcaa ccggatcgtc
      241 gaactgctcg gcggcgagaa gatccacata ccccggcggc cgggcgaacc ggactgcacc
      301 cacgcggaca tcagccggat caccgccgag ctcggctggc ggcccgaagt cagcctcgag
      361 gccggcgtga agatcatgct ggagaatatc gattactgga ggcaggcccc gctctggacc
      421 ccggaaaaga tcgccgaggc cacccgggac tggttcaccc acctggacag gaaagaaccg
      481 tgaccgaatc gcccaagctg aaggagctcg accaactggc ccggaccctg gccgccctga
      541 aaggaaaggg gcggaccatc gtccactgcc acggggtctt cgacctgctc cacccgggcc
      601 acatcaagca tttccaggcc gcccgcaagc tgggcgacgt cctggtggtc accgtgacgc
      661 cggacgagta cgtcaacaag ggtccgggcc ggccggtctt caaccagcgg ctgcggctgg
      721 aatcgatcgc cgcgctcgaa tgcgtcgatt acgtggccct caaccgctgg ccgagcgccg
      781 aggagacgat ccggcggctg aaaccggaca tctacgtcaa gggcagcgat tacgccgagt
      841 cgaacaagga cctcaccggc aagatcaacg acgagcgccg ggcggtggaa tcggtcggcg
      901 gccggatcca cttcaccgac gaggtcacct tctcctccac ccggctggtg aacgccttct
      961 tcagcgtcta cccggaggag gccggccgct tcctggaggg cttccgccgg cgccattcgg
     1021 ccgaatcggt catcggccgc ctccaggagc tggccggcac ccgggccctg gtcatcggcg
     1081 acgccatcat cgaccagtac gcctactgcc agccgatggg aaaggccggc aaggagagcg
     1141 tcctggtcgc ccgccacctc tcccaggagg agttcgccgg cggcgcgctg gcctgcgcca
     1201 accacctggc cggcttctgc cgcgaggtga ccctggtcac cggcctcggc cgcggcaatt
     1261 cccgggagaa gttcatccgg gaacgcctcc gccccaacat caagccggtc ttcttcagcc
     1321 ggagcgacgc gccgaccgtg gtcaagcgac gcttcgtcga gccgcccttc ctctccaagc
     1381 tcttcgaaat ctgctacctg aacgacgcca acctgccggc cgaggttgaa aaacggctgg
     1441 ccgctttcct ggaacgggaa ctgccccgct acgacctggt ggtcgccgcc gacttcggcc
     1501 acggcttcat caccccggcc accgtgggac tgctcggccg gcgcgctccc ttcctggccc
     1561 tcaacaccca gaccaacagc gccaactccg gctacaacct ggtcacccgc taccgccggg
     1621 ccgactacat ctgcgtggac gaaccggaga tgcgcctggc caaccaggac cgcttcggcc
     1681 gcctggagaa gatcatccag gcgaccggaa agcagctgga ctgccggcgg accatcgtca
     1741 ccc
//
LOCUS       DAOQYN010000294         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_83084_length_1739_cov_5.205463, whole genome shotgun sequence.
ACCESSION   DAOQYN010000294 DAOQYN010000000
VERSION     DAOQYN010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83084_length_1739_cov_5.205463"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PKN80_08985"
     CDS             complement(<1..474)
                     /locus_tag="PKN80_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNQ36180.1"
                     /translation="MIRIAVDLAGSEQGPAAVLAGARKARAEAPDLEITLVGPRTGLS
                     EPELAEFKYLEAPVAIPMCEKVTRELLRNPDSSLFKIVRLVKEGEADAAVSGGNTACF
                     VALATSMLGTIPGVDRPGIAIFLPKLNGSTILLDAGANTATSAEHLVSYAVMGHLL"
     gene            complement(471..668)
                     /gene="rpmF"
                     /locus_tag="PKN80_08990"
     CDS             complement(471..668)
                     /gene="rpmF"
                     /locus_tag="PKN80_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNQ36181.1"
                     /translation="MPNPFRKHSHSRGAKRRSANRLKAKGTTLCGNCSEPVLSHRVCP
                     ACGYYRGKPALIVKTKKEKKA"
     gene            complement(685..1173)
                     /gene="coaD"
                     /locus_tag="PKN80_08995"
     CDS             complement(685..1173)
                     /gene="coaD"
                     /locus_tag="PKN80_08995"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNQ36182.1"
                     /translation="MDTVVYPGSFDPITLGHLDIIERARGLFPRVIVAVADNSEKRPI
                     FTAAERLELIRTSAADIPGVETDTFTGLLVDYLEKAGCFTILRGLRAVSDFDYEFQML
                     LTNRRLLPRVETIFLMPQEKFFFLSSSLIKELARMNADLRDFVPPSVERALKAKLAVK
                     KA"
     gene            1351..>1739
                     /locus_tag="PKN80_09000"
     CDS             1351..>1739
                     /locus_tag="PKN80_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNQ36183.1"
                     /translation="MPEPVTGKPVWQLSISELESALGSSGKSGLDPETAGRRLSEYGP
                     NQLQEKAATSPWRLFFAQFEDFIIWVLIGAALVSGFLKEWVDALAILGIVVLNSVLGF
                     IQEYRAEKSLAALKKLASPVARVWRGG"
BASE COUNT          305 a          547 c          564 g          323 t
ORIGIN      
        1 gagaaggtgg cccatgaccg cgtatgagac caggtgttcg gcgctggtgg cggtgttggc
       61 cccggcgtcc agcaggatgg tggagccgtt cagcttgggc aggaaaatgg cgatacccgg
      121 ccggtcaacg cccggtatcg ttcccagcat ggaagtggcc agggccacga agcaggcggt
      181 gttgccgccg ctgaccgcgg catcggcctc tccttccttg accagccgga cgatcttgaa
      241 aagagaggag tcggggttgc gcaggagttc ccgggtcacc ttctcgcaca tcgggatggc
      301 gaccggggcc tccaggtact tgaactcggc cagctccggc tcggacaggc cggtccgcgg
      361 gccgaccagg gtgatttcca ggtcgggcgc ctcggcccgc gccttccggg cgccggccag
      421 cacggcggcc ggaccttgct cgctgccggc caggtccacc gcgatccgga tcatgccttt
      481 ttctctttct tggtcttgac aatcagcgcc ggcttgcccc ggtagtaacc gcaggccggg
      541 cagacccggt gcgacaggac cggttcgctg cagtttccgc agagggtcgt cccctttgcc
      601 ttgagccggt tggcgctgcg ccgtttggcg ccgcgggaat gagagtgctt gcggaaagga
      661 ttcggcatct tggacctcct cgggtcaggc ttttttaacg gccagtttcg ccttgagggc
      721 ccgttccacc gagggcggaa cgaagtcccg caggtcggca ttcatccggg ccagttcttt
      781 aatcagactg gaactcagaa agaaaaactt ttcctgcggc atcagaaaaa tggtctccac
      841 tctcggcagc agccggcggt tggtcaggag catctgaaac tcgtagtcga aatcggagac
      901 cgcccgcagg ccgcgcagga tggtaaagca gcccgccttt tcgagataat caaccaggag
      961 gccggtgaag gtgtcggtct ccacgcccgg gatgtcggcg gccgaggtcc ggatcagctc
     1021 cagccgttcg gcggccgtga agatcggccg cttctccgag ttgtccgcca ccgccacgat
     1081 cacccgggga aaaaggcccc gggcccgctc gataatgtcc aggtggccca gggtgatcgg
     1141 gtcgaaactg ccgggataga ctacggtatc aatcattcgg gtaatatttt accatagccg
     1201 caagccgctt cacaacccgg gtacctttgc cgccggcgcg ccttatggca taaaatatag
     1261 aatacggccg cggtcggttt gaaccgaccg ctatcggccg gcgccggccg ccaagaccgg
     1321 cggattgatc aatctaccca ggagaaattc atgcccgaac ccgtcacagg aaaacccgtc
     1381 tggcagcttt ccatcagcga gctggaatcc gcgctgggta gcagcggcaa gtccggcctg
     1441 gacccggaaa cggccggccg ccgcctctcc gagtacgggc ccaaccagct gcaggagaag
     1501 gcggcgacca gcccctggcg gctcttcttc gcccagttcg aggatttcat catctgggtc
     1561 ctgatcggag ccgcccttgt ctccggcttt ctgaaggagt gggtcgacgc cctggccatc
     1621 ctgggcatcg tggttctcaa ttcggtgctc ggttttatcc aggagtaccg ggccgagaag
     1681 tccctggccg ccctgaagaa gctggccagc ccggtcgccc gggtctggcg gggcggctg
//
LOCUS       DAOQYN010000295         1722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_84439_length_1722_cov_3.490702, whole genome shotgun sequence.
ACCESSION   DAOQYN010000295 DAOQYN010000000
VERSION     DAOQYN010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84439_length_1722_cov_3.490702"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PKN80_09005"
     CDS             <1..776
                     /locus_tag="PKN80_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNQ36184.1"
                     /translation="MKDFGGYEHNRQGLRVVTKLEKRYPEFDGLNLTFEVREGIIKHV
                     SEYDQPEVQEYDPKLYPTLEAQVVNLADEIAYTSHDVDDGLKAGLISAEDLDRVRLWR
                     DLYGKVSGRGLEPELARYAAVRDLINLLVTDLLEQTGRNISGRRIRSVEAVRRQAQPL
                     VEFSPATARKHQELKKFLYENMYRHHRVIRMSEKAKRIIGDLFKVYTKQPKQLPPQVA
                     GRISSAEPAARVVCDYIAGMTDNFALEEYQKLFDPFTRV"
     gene            776..>1722
                     /gene="dprA"
                     /locus_tag="PKN80_09010"
     CDS             776..>1722
                     /gene="dprA"
                     /locus_tag="PKN80_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNQ36185.1"
                     /translation="MEKDLLWWVALNLVPAIGPVKAGRLLDHFGSPEAVFRAGRGDLR
                     AVSGLWASDVEAVAGFNPAAAEAELRLVEKHQFKLLTREDPGYPAPLRNIADPPILLY
                     LKGEITAEDHPACAIVGTRICSPYGRVQAERFASALAAYQVTVVSGLARGVDTAAHLG
                     ALRGRGRTLAVLGSGLLNIYPEENRPLAARIASSGAVISEFPLSVRPDKGTFPRRNRL
                     ISGLSGGVLIVEAGQRSGALITTDYALEQGREVFALPGPVDQETSTGTNRLIADGARP
                     ALVPEDIVSVLVPNRLAPAAPAAGTETAGLPFDDELTGRL"
BASE COUNT          283 a          596 c          586 g          257 t
ORIGIN      
        1 tgatgaagga tttcggcggc tacgagcaca accgccaggg gctgcgggtg gtgaccaagc
       61 tggagaagcg ctacccggag ttcgacggcc tcaacctgac cttcgaggtg cgcgaaggca
      121 tcatcaagca tgtctcggag tatgatcagc cggaggtgca ggagtacgat ccgaaactct
      181 acccgaccct ggaggcccag gtggtcaacc tggccgacga gatcgcctac accagccacg
      241 atgtggacga cggcctgaag gccggcctga tcagcgccga ggacctggac cgggtgcgcc
      301 tctggcgcga tctttacggc aaggtctccg gccgcggact ggagccggag ctggcccggt
      361 acgcggcggt gcgcgacctg atcaacctgc tggtgaccga cctgctggag cagacgggcc
      421 ggaacatctc cggccgccgg atcaggagtg tcgaggcggt ccggcgccag gcccagccgc
      481 tggtcgagtt cagcccggcc acggcccgca agcaccagga gctgaagaag ttcctttacg
      541 agaacatgta ccggcaccac cgggtgatcc ggatgtcgga gaaggccaag cgcatcatcg
      601 gcgatctctt caaggtttat accaagcagc ccaagcagct gccgccccag gtcgccggcc
      661 gcatcagcag cgccgagccg gccgcccggg tggtctgcga ctacatcgcc ggcatgaccg
      721 ataacttcgc cctcgaggag taccagaagc tcttcgaccc attcacccgg gtgtgatgga
      781 gaaggacctg ctctggtggg tggccctcaa cctggtgccg gcgatcgggc cggtcaaggc
      841 cggccggctg ctggatcatt tcggcagccc ggaggcggtc ttccgggccg gccgcggcga
      901 cctgcgggcg gtgagcggcc tctgggcctc ggacgtcgag gcggtggccg gcttcaaccc
      961 ggccgcggcc gaggccgaac tgcggctggt cgagaagcat caatttaaac tgctgacccg
     1021 cgaggacccc ggttaccctg ccccgctcag gaacatcgcc gacccgccca tacttctcta
     1081 tctcaagggt gaaatcaccg ccgaagacca cccggcctgc gccatcgtcg gtacccgcat
     1141 ctgcagtcct tacggccgcg tccaggccga gcggttcgcc tcggccctgg ccgcctacca
     1201 ggtgacggtg gtaagcggtc tggcccgggg cgtcgatacc gcggcccacc tgggcgccct
     1261 gcgcggccgg ggacggacgc tggcggtgct gggcagcggg ctgctgaaca tctatcccga
     1321 ggagaaccgg ccgctggccg cccggatcgc ctccagcggc gcggtgatct cggagttccc
     1381 gctttcggtg cggccggaca agggcacctt tccgcgccgc aaccgcctta tcagcggcct
     1441 ctccggcggc gtgctgatcg tcgaagccgg ccagcgcagc ggcgccctga tcaccaccga
     1501 ttacgccctg gaacaggggc gcgaggtctt cgccctgccc ggcccggtcg accaggagac
     1561 cagcaccggg accaaccgcc tgattgccga cggcgcccgt ccggccctgg ttccggagga
     1621 catcgtttcg gtgctggtcc cgaaccggct ggcgccggcc gcgccggccg ccgggacgga
     1681 gacggccgga ttgcctttcg atgacgagct gaccggccgc ct
//
LOCUS       DAOQYN010000296         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_84587_length_1720_cov_4.387988, whole genome shotgun sequence.
ACCESSION   DAOQYN010000296 DAOQYN010000000
VERSION     DAOQYN010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84587_length_1720_cov_4.387988"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            451..777
                     /locus_tag="PKN80_09015"
     CDS             451..777
                     /locus_tag="PKN80_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 domain-containing protein"
                     /protein_id="HNQ36186.1"
                     /translation="MPKLIWHFILGGAVVSLATHFGSKGEGKLAAFITQFPSISVLAL
                     FLIYQGGGAPAVGKYVQGFLYVIPPWLLYVFAVWVLCARIGIIPSLACGVGLYFGVSW
                     LLVGLK"
     gene            complement(810..1112)
                     /locus_tag="PKN80_09020"
     CDS             complement(810..1112)
                     /locus_tag="PKN80_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNQ36187.1"
                     /translation="MPEFGSPFSGLKSGRPVTKEELIRAIRFMIAAEYEAIQLYMQLA
                     ESTDHKLAQEVLKDVADEERVHAGEFLRLLKELDPDEEKFYAEGAEEVEAEIEKDR"
     gene            1334..>1720
                     /locus_tag="PKN80_09025"
     CDS             1334..>1720
                     /locus_tag="PKN80_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36188.1"
                     /translation="MQKRWFGVVGLCLPVGLFLLLTGCAHPPRVPAPPALIESRISAY
                     GLTVTVESTPREVTALLLRGLDNGDGDLLARLAAVEDIMAELERIYRRYGKDSKLTRE
                     QVVQVAVSGWPAASGPAAGPGCADGRH"
BASE COUNT          288 a          502 c          547 g          383 t
ORIGIN      
        1 ggacttgaag agagcgaagt caggcaaggc gaccgaatag ggagcgttgc cagcgggtcc
       61 cgactacgct ttacaaaagc ttcgtcgggt aaaggccgac cttgcctgcc gaagtcagcc
      121 tacgctaaag cttcgggaga taaatcggcc tgcgctgaaa agcagcttcg gctgataaac
      181 tttgacgcag gcacggccga gccgcagtgg gaggcgaggg cgccaagccc cgacccggca
      241 cggtgaagta gagcgcctcg acgagctcgg cgcacccttc accgccgtga agcgtgtccg
      301 aaggactctg cttcacggcc tcgttgtttc tgaactgtat ttcagacgga ttttaaataa
      361 cagaggggga ctcgctttgc tcgctcacgg caggctgatt tcgagtcgaa cggcacgcat
      421 ctttgttttc tcgtcaaaag gcgggtaacc atgcctaaat taatctggca ctttatcctg
      481 ggcggggcgg tggtcagtct ggccacccat ttcggctcga agggcgaggg gaagctggcg
      541 gccttcatca cccagtttcc atccatctcg gtcctggccc tcttcctgat ctaccagggc
      601 ggcggcgcgc cggcggtggg caagtacgtc cagggattcc tgtatgtgat cccgccctgg
      661 ctgctttacg ttttcgccgt ctgggtcctc tgcgcccgga tcgggatcat cccgagcttg
      721 gcctgcgggg tcggactcta cttcggggtc tcctggcttc tggtggggct caagtagccg
      781 ggccgggtcc gctaacgttt ccagagcggt cacctgtcct tttcgatctc cgcctccacc
      841 tcttcggccc cttcggcgta gaacttctcc tcgtccgggt ccagttcctt cagaaggcgg
      901 agaaactcgc cggcgtggac gcgctcctcg tcggcgacgt ccttcagcac ctcctgcgcc
      961 agcttgtggt cggttgactc ggccagctgc atgtagagct ggatggcctc gtactcagcg
     1021 gcgatcatga aacggatggc gcggatcagt tcctccttgg tgacgggccg gccgctttta
     1081 aggccggaaa acgggcttcc aaattcaggc atggcatcct cccggtaggc gtgatgattg
     1141 tcgttccggg tttattatac tacaggtccg tccggaccgg ttgagctatc cgatctttcg
     1201 gcagattgag ccggcggctt ctccagcagt tccggcgtct cttgacggct ttgacccggt
     1261 ccggtccagg tgataatatt gagttgaaaa agcttttcag atcgggctga aaaaacttgt
     1321 tccggaggtc cctatgcaga agagatggtt cggggttgtt ggtctctgcc tgccggtcgg
     1381 cctgttcctg ctcctgaccg ggtgcgccca cccgccccgg gtcccggcgc caccggcctt
     1441 gatcgaaagc cggatttcgg cttacggcct gaccgtgacg gtggagagca cgccccggga
     1501 ggtaaccgcc ctactgctcc gcggcttgga taacggagac ggtgatttgc tggcccggtt
     1561 ggcggcggtc gaagatatta tggccgagct ggaaaggatt taccggcgtt acgggaagga
     1621 ttcgaagttg accagggaac aggtggtcca ggtggcggtt tcgggttggc cggccgcttc
     1681 tgggcctgct gccgggcctg ggtgtgccga tggccgccac
//
LOCUS       DAOQYN010000297         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_85531_length_1708_cov_4.287961, whole genome shotgun sequence.
ACCESSION   DAOQYN010000297 DAOQYN010000000
VERSION     DAOQYN010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85531_length_1708_cov_4.287961"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1708)
                     /locus_tag="PKN80_09030"
     CDS             complement(<1..>1708)
                     /locus_tag="PKN80_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36189.1"
                     /translation="LITSFTGVDAHDRFLLGSYLWSTSGVPEGVYSIRIEVQDDVYTD
                     SVLSPGKVQVFTVLPSLVNPAAVSLVSPSGLQGILPASGTTAVLGLNLPAQLDEVVYL
                     DTLLVTLIDEELTDSRQIEDYLGIVLYRDDDANTDTGWFSHANDSQISLVQVGSWVET
                     IIGGQRRVQGVFNLAASDLLPDNDLSQNGGYDYYLAVQAADGLPFGEAFRVQLDSIFI
                     KSGALQAEVPFASATALITQGSRLTDLSAGDTNYNYSIPEELAEPGREYLITELAAYA
                     TPANIQKYLAKRVILTQNTPTAVLAISLAGNGGEQSGVGIYQNELLESLALNFNDVGE
                     PNFDPQLHLAAIDLYRDNGDGVFGVTDTRLPAAIGWTPGQARLTLETPEPVTGLLPAA
                     PNYFVVFNVGGSVSAGADFKVSVESGGFEFAYAAPVDGLKLDSSLVDTQLVSLEIHDY
                     APALVDATSSSIPMLGLNLADGIGLNDELVELKVTFGGTGFEPDDLAPLNGDATGGIL
                     LYRDRSGSGREGVFDPNDMVIPIQNGVSSPWVDNGDGTYSVTIRPTSALVLPDADAGA
                     DRG"
BASE COUNT          296 a          530 c          593 g          289 t
ORIGIN      
        1 gccgcggtcc gcgccggcgt cggcgtcggg caacacgagg gccgaggtgg gccggatggt
       61 gaccgagtag gtcccgtcgc cgttgtccac ccaggggctg ctgaccccgt tctggatcgg
      121 aattaccatg tcgttcgggt caaagacgcc ctcgcgcccg gaaccggagc ggtcccggta
      181 gagcaggatg ccgccggtgg cgtcgccgtt gagcggcgcc aggtcgtcgg gttcaaagcc
      241 ggtcccgccg aaggtgacct tcaactccac cagctcgtcg ttcagaccga tcccgtcggc
      301 caggttcagc cccagcatcg ggatcgaaga gctggtggcg tcgaccaggg ccggcgcgta
      361 atcgtggatt tccaggctga ccagctgggt atcaaccagg ctgctgtcga gcttgagccc
      421 gtccaccggg gcggcgtagg cgaattcaaa gccgccgctc tcgaccgaaa ccttgaagtc
      481 ggccccggcg ctgacgctgc cgcccacgtt gaagaccacg aaatagttgg gcgcggccgg
      541 cagcaggccg gtgaccggct ccggcgtctc gagagtgagc cgggcctgtc cgggcgtcca
      601 gccgatcgcg gccggcagcc gggtgtcggt gacgccgaag acgccgtcac cgttgtcgcg
      661 gtaaagatcg atcgccgcga ggtggagctg cgggtcgaag ttgggttcgc ccacgtcgtt
      721 gaaattgagg gcgagggact ccagcagctc attctggtaa atcccgaccc cggactgctc
      781 gccgccgttg ccggccaggc tgatggccag gaccgccgtg ggcgtgttct gggtcaggat
      841 aacccgcttg gccaggtact tctggatatt cgccggggtg gcgtaggcgg ccagctcggt
      901 aatcaggtac tcccggcccg gctcggccag ttcttccggt atcgagtaat tgtaattggt
      961 gtcgccggcc gaaagatcgg tcaaccggct gccctgggtg atcaaagcgg tggcggaggc
     1021 aaagggaacc tcggcctgaa gcgccccgct cttgatgaag atgctgtcca actggacccg
     1081 gaaggcctcc ccgaagggca gcccgtcggc ggcctggacg gccaggtagt aatcataacc
     1141 gccgttctgg ctgaggtcat tatccggcag caggtcggag gcggccaggt tgaagacgcc
     1201 ctggacgcgc cgctgcccgc cgatgatcgt ttcgacccag ctgccgacct ggacgagcga
     1261 aatctggctg tcgttggcat ggctgaacca gccggtatcg gtattggcgt catcgtcccg
     1321 gtagaggacg atgccgaggt aatcttcgat ctgccggctg tcggtcagct cctcatcgat
     1381 cagagtcacc aggagcgtgt ccaggtaaac gacctcgtcg agctgggccg gcaggttgag
     1441 acccaggacc gcggtcgtcc cgctggccgg cagaatgccc tggagtccgg agggcgaaac
     1501 cagcgaaacc gccgccggat ttaccaggct cggcagcacg gtgaatacct gcaccttgcc
     1561 gggggagagg accgaatcgg tatagacgtc gtcctggacc tcgatccgga tcgaatagac
     1621 gccctccggc accccggagg tgctccagag atagctgccc agcaggaagc ggtcgtgggc
     1681 atcgacgccg gtgaagctgg tgatcagg
//
LOCUS       DAOQYN010000298         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_86775_length_1693_cov_2.778999, whole genome shotgun sequence.
ACCESSION   DAOQYN010000298 DAOQYN010000000
VERSION     DAOQYN010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86775_length_1693_cov_2.778999"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PKN80_09035"
     CDS             complement(<1..760)
                     /locus_tag="PKN80_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ36190.1"
                     /translation="MLLVLKLAWRNVHRNTRRSILTVLIFAVGLATLVISNALYDGFH
                     EKMVINAVRIFIGHLQVHAEGFHANPTVDKCFVPPPEKNLDLPQVTALARRVRFQSLA
                     STAATSQGVMVVGIEPEREAQVTNLKKSLVSGTYLGSGAEDRQSCLVGERLMESLRLE
                     PGEKLVLFGQAYDGSIAAAACRVSGVCRTGNPEIDRSFVWMPIVAAQEMLGYGDRISE
                     LVFLTRRSNEVPAAERELERRLAGAGLEVLSWKEV"
     gene            complement(762..1511)
                     /locus_tag="PKN80_09040"
     CDS             complement(762..1511)
                     /locus_tag="PKN80_09040"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNQ36191.1"
                     /translation="MLAVKGLLLLLLVGPAAAPADEPALSATEVIRRMELQTRGESSQ
                     GELDLEIVSPSFQRRLGIRFWTQGNEKALVRVTAPSKEAGIGWLRLGDLMWNYFPKVE
                     RTIKIPPSVMMQPWNGSDFTNDDMMKASSFVTDYRHRLLRARPDAEGYRWEIECVPRP
                     DRPVVWGKLIFRVNADFLPVRQEFYDERGQLVKSLDFSDVRKVGERPFPHTWTMTNLK
                     KPGCRTVIRYREVAFDRTLPEDTFSLRRLSR"
BASE COUNT          307 a          558 c          541 g          287 t
ORIGIN      
        1 tgacctcctt ccagctcagc acctcgaggc cggcgccggc cagccggcgc tccagttccc
       61 gctcggccgc cggcacttca ttcgagcggc gggtcaggaa gaccagctcc gagatgcggt
      121 ccccgtaacc gagcatctcc tgggcggcca cgatcggcat ccagacgaag gaacggtcga
      181 tctccgggtt gccggtccgg cagacgccgc tcacccggca ggcggccgcc gcgatcgaac
      241 cgtcgtaggc ctggccgaag aggacgagct tctcgcccgg ctccagacgc aggctctcca
      301 tgagccgttc gcccaccagg caggactgcc ggtcctccgc tcccgaaccc aggtaggtcc
      361 cggagaccag cgacttcttc aggttggtga cctgcgcctc ccgctccggc tcgatgccga
      421 ccaccattac cccctgcgag gtggcggcgg tactggccag cgactggaaa cggacccggc
      481 gggccagggc ggtcacctgg ggcaagtcga ggttcttttc cggcggcggc acgaaacact
      541 tgtccacggt cgggttggcg tgaaatccct cggcgtggac ctggaggtgg ccgatgaaga
      601 tccggaccgc gttgatcacc atcttttcat gaaaaccgtc gtagagggcg ttggagatga
      661 ccagggtggc caggccgacg gcaaaaatca ggacggtgag tatcgaacgg cgggtgttgc
      721 gatggacatt ccgccaggcc agcttcagga ccaggagcat ctcagcggga gagacggcgg
      781 agcgagaagg tatcttccgg cagcgtccgg tcgaaggcca cttcccggta ccggatcacg
      841 gtccggcagc ccggtttttt caggttggtc atcgtccagg tatggggaaa cggccgctcg
      901 ccgaccttgc gcacgtccga aaaatcgagg gactttacca gctggccacg ctcgtcgtag
      961 aactcctggc ggaccggcag gaaatcggca ttcacccgga agatcagctt tccccagacc
     1021 accggccggt ccggccgggg cacgcactcg atctcccagc ggtatccctc cgcatccggc
     1081 cgggcccgca gcaggcggtg ccggtaatcg gtgacgaaac tgctggcctt catcatgtcg
     1141 tcattggtga aatcgctccc gttccagggc tgcatcatca ccgagggggg aatcttgatg
     1201 gtccgttcca ccttggggaa gtaattccac atcaggtcgc ccaggcgcag ccagccgata
     1261 cccgcctcct tgctcggcgc ggtcacccgg accagggcct tctcgttgcc ctgggtccag
     1321 aaccggatcc ccagccggcg ctggaaactg ggggagacga tctccaggtc gagttccccc
     1381 tggctgctct cgccgcgggt ctgcaattcc atgcgtcgga tgacttcggt cgcggaaaga
     1441 gcgggctcgt ctgccggcgc cgcggccggt ccgaccagca ggagaagcag cagtcctttt
     1501 accgccagca ttcttctcat cgttgccctg tcaaggaatc ttccgggacc agttcggaac
     1561 cggcggaaat tgacggaact caggactcgc ccccggcgta aacataacgc tggtaggacg
     1621 gctggctgat ccgccgcggg aagaagctcc tcaccttcag cagggtggcg taaaggaaga
     1681 agaagaccgc ctg
//
LOCUS       DAOQYN010000299         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_87790_length_1681_cov_3.321648, whole genome shotgun sequence.
ACCESSION   DAOQYN010000299 DAOQYN010000000
VERSION     DAOQYN010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87790_length_1681_cov_3.321648"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PKN80_09045"
     CDS             <1..334
                     /locus_tag="PKN80_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36192.1"
                     /translation="PLEEWPRLFPIQVACGVIGTASLARIELIENNRVVYTHLRWRGP
                     ASQMAFQLKFETVTYYPRYYYVRVTQTDGQMAWSSPVFIDYRVPPDMYYWQSVPADRL
                     ARNKRRTE"
     gene            331..705
                     /locus_tag="PKN80_09050"
     CDS             331..705
                     /locus_tag="PKN80_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36193.1"
                     /translation="MKKSLDPAVVLGDRFREFQVQFWDYATLSLLKAFAAICEKGGFG
                     RIDSFTGAGRIRSGARYRFQWRKGAPLEIKADQESSEAAGGLVLKGKLDFFTINRIRN
                     FPLFHLRGLYRKPIAARWGYHD"
     gene            698..>1681
                     /locus_tag="PKN80_09055"
     CDS             698..>1681
                     /locus_tag="PKN80_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36194.1"
                     /translation="MIKLEGRIKCPFCYAYFPRRAGAVQQCFDCGREFEPVRLVRASR
                     FRLDGRQLTVAEAVNHPVRPIRHGFAHESFRHPELETLRKKYRLDEIGKNAPDEFSRQ
                     LALRDWVSAAWTSGQPSVRHPGNGGPDHDYLASVMLEKSRRSGHCYFCTYKALASVQL
                     ASAFGWTARLINCDGQHSPAGPANCGHMVYEIWSNQFNKWYLNDSLFNVHYEKEGRPL
                     SIYELRREFLTNRSRAVRQVWGPGRHPMPREVHGGFGAWSFEWYVVYLHNNLLDFPAG
                     DHIHPLLMPRDRFNRNRNWRRFDPKTGTGRSNVYVCKGMVIEESDPYHLNYP"
BASE COUNT          294 a          587 c          519 g          281 t
ORIGIN      
        1 gcccctggag gagtggcccc gtctcttccc gatccaggtc gcctgcgggg tgatcgggac
       61 ggccagcctg gcccggatcg agctgatcga gaacaaccgg gtcgtctaca cccacctgcg
      121 ctggcgcggt ccggccagcc agatggcctt ccagctgaag ttcgaaacgg tcacctacta
      181 cccccgctac tactatgtcc gggtcaccca gaccgacggc cagatggcct ggtccagccc
      241 ggtcttcatc gactaccggg tacccccgga catgtactac tggcaatcgg tccccgcgga
      301 ccggctcgcc agaaacaaaa ggagaacgga atgaagaagt ccttggaccc ggcggtcgtc
      361 ctgggcgacc ggttccggga atttcaggtg cagttctggg attatgccac cctctcgctc
      421 ctgaaggcct tcgccgccat ctgcgaaaag ggcggcttcg gccggatcga cagcttcacc
      481 ggcgccggcc ggatccgctc cggcgcccgc taccgcttcc agtggcgcaa gggcgccccg
      541 ctggaaatca aggcggacca ggaaagctcc gaggcggccg gcggcctggt cctgaaaggg
      601 aagctcgact tcttcaccat caaccggatc cggaacttcc cgctctttca cctgcgggga
      661 ctctaccgga aaccgatcgc tgcccgttgg gggtaccatg attaaactcg aaggccggat
      721 caagtgtcct ttctgttacg cctactttcc gcgccgggcc ggggcggtcc agcagtgctt
      781 cgactgcggc cgggagtttg agccggtccg gctggtccgg gccagccgtt tccgcctgga
      841 cggcaggcag ctgaccgtgg ccgaagcggt caatcatccg gtgcggccga tccgccacgg
      901 cttcgcccac gagagcttcc gccatcccga actggaaacg ctccggaaga agtaccggct
      961 ggacgagatc gggaagaacg ccccggacga gttcagccgg cagctggccc tgcgtgactg
     1021 ggtcagcgcc gcctggacct ccggccagcc ctcggtgcgc cacccaggca acggcggacc
     1081 cgaccacgac tatctcgcgt ccgtgatgct ggagaagtcc cgccggtccg gccactgcta
     1141 cttctgcacc tacaaggccc tggcctcggt ccagctggcc tcggccttcg gctggacggc
     1201 ccggctgatc aactgtgacg gccagcacag tccggccggc ccggccaact gcggccacat
     1261 ggtttacgag atctggtcca accagttcaa caagtggtac ctgaacgact cgctcttcaa
     1321 cgtccattac gagaaggagg gccggccgct ctcgatctac gaactgcggc gcgagttcct
     1381 gaccaaccgg agccgggcgg tgcggcaggt ctggggtccg ggccgccatc cgatgccccg
     1441 cgaggtccac ggcggtttcg gcgcctggag tttcgagtgg tacgtggtct atctccacaa
     1501 caacctgctc gacttcccgg ccggcgacca catccacccg ctgctgatgc cgcgcgaccg
     1561 cttcaaccgc aaccggaact ggcgccgctt cgacccgaag accgggaccg gccgcagcaa
     1621 cgtctatgtc tgcaagggga tggtcatcga ggagtcggat ccctaccacc tgaattaccc
     1681 g
//
LOCUS       DAOQYN010000300         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_87795_length_1681_cov_3.169127, whole genome shotgun sequence.
ACCESSION   DAOQYN010000300 DAOQYN010000000
VERSION     DAOQYN010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87795_length_1681_cov_3.169127"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKN80_09060"
     CDS             complement(<1..662)
                     /locus_tag="PKN80_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNQ36195.1"
                     /translation="MKIRSRYPGHRFKKTMKAWIILAVFLAIGPGAEAVERRVRSERA
                     VNGLDLVMEEVPGTELVNLSLLVKTGSATEGDYAGTGITHLLEHLIFKPGEQTGLAET
                     VERTGGYTNAFTSLDSTVFTATVPAGAWQEVLPVLLDAVFKPAFNESDFEKEREVVLR
                     ELSRNGDNPAQAASRGLWETAFLAHPYRNPVGGRPERLKGLSFKDLTTYHRRTYQPGN
                     AIL"
     gene            complement(665..904)
                     /locus_tag="PKN80_09065"
     CDS             complement(665..904)
                     /locus_tag="PKN80_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNQ36196.1"
                     /translation="MSRRIKVRVIPGARRTRIDAEPDGRLRVHLSAPPLDGRANRALV
                     EILASHFGVKKNRVSIIAGERNREKLIQIDDSTGG"
     gene            complement(901..1473)
                     /locus_tag="PKN80_09070"
     CDS             complement(901..1473)
                     /locus_tag="PKN80_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNQ36197.1"
                     /translation="MNHQCARRFLRPAAVLLTGTALLLAGAFLRTQATGNFQPVIPAR
                     LYRSGQLSAGQLETRIRQCGLKSVVNLRGRNETSGWWRQETELCRRLGVAHLDFTLSS
                     RKPAGPEQLDRLAELLRSAPKPALVHCQGGADRTGLAAAVYVYEVEGLDYERARRQLA
                     ARYGHLPFFKPGSFAMDRSFEAFRRHRERG"
     gene            1514..>1681
                     /locus_tag="PKN80_09075"
     CDS             1514..>1681
                     /locus_tag="PKN80_09075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNQ36198.1"
                     /translation="MPTTLRELRDMAAPATADLLINLHVEVPPFFSQFEQVVETTGED
                     EQAKALARTRYK"
BASE COUNT          275 a          508 c          588 g          310 t
ORIGIN      
        1 aggatggcgt tgccgggttg ataggtgcgg cggtggtagg tggtcaggtc tttaaagctc
       61 agtcccttga gccgttccgg ccggccgccg accgggttcc ggtaaggatg cgccaggaag
      121 gcggtctccc agaggccgcg gctggcggcc tgggccgggt tgtcgccgtt gcgcgagagt
      181 tctctaagga cgacctcccg ttctttttcg aagtcgcttt cgttgaaggc gggcttgaag
      241 accgcgtcca ggaggaccgg caggacttcc tgccaggccc cggccggaac cgtggcggtg
      301 aagacggtgc tgtccaggga ggtgaaggcg ttggtatagc cgccggtccg ttccaccgtt
      361 tcggccagcc cggtctgttc gcccggtttg aagatgagat gttccagcag gtgggtgatc
      421 ccggtgccgg catagtcacc ctcggtggcg gagccggttt tgaccagcag cgagaggttg
      481 accagttccg ttcccggtac ttcttccatg accaggtcca gtccgttgac ggcccgttcc
      541 gagcggaccc tccgttcgac cgcctcggcc ccgggcccga tggccaggaa gacggccagg
      601 atgatccagg ccttcatggt ctttttaaag cgatggccgg ggtaacggct tcggatcttc
      661 attttcagcc tccggtcgaa tcatcgatct ggatcagctt ctcccggttc cgttcaccgg
      721 caattatgga gacccggttt tttttgaccc cgaaatgact ggccagtatt tcaaccaggg
      781 cccggttggc ccggccgtcc aggggcgggg ccgacaggtg aacccggagc cggccgtccg
      841 gttcggcgtc gatccgggtt cgccgggcgc ccggaatcac ccggactttg atgcggcggc
      901 tcatccccgt tcccggtggc gccggaacgc ttcgaatgaa cgatccatgg caaaggaacc
      961 gggcttgaag aaaggcaggt gcccgtaacg ggccgccagc tgccgccggg cgcgttcgta
     1021 gtccaggcct tccacctcgt aaacatagac ggcggcggcc agtccggtgc ggtcagcccc
     1081 gccctggcaa tggaccagcg ccggcttggg cgcgcttcgc aaaagctcgg ccagccggtc
     1141 gagttgctcc ggaccggccg gttttcggga ggagagcgta aaatccaggt gggcgacgcc
     1201 cagtcggcgg caaagttcgg tctcctgtcg ccaccacccg gaggtttcgt tgcggccgcg
     1261 caggttgacg acgcttttga ggccgcactg ccggatgcgg gtttcgagct ggccggcgga
     1321 gagctggccg gaacggtaaa gacgagccgg gatgacgggc tggaaattac cggtggcctg
     1381 ggtccggagg aaggcgccgg cgaggagcag ggccgttccg gtcagcagga cggcggcggg
     1441 ccggagaaac cgcctcgcgc attgatgatt catttaatcc tgccgcgtcc ggaatcgttt
     1501 gaggagcagc gagttgccga ccacgctgag ggaactcagg gacatggcgg cgccggccac
     1561 ggccgatctg ctgatcaatc tccacgtcga ggtgccgccg ttcttcagcc agttcgagca
     1621 ggtcgtcgag acgaccggtg aagacgaaca ggcgaaggcg ctggcccgca cgcgctacaa
     1681 g
//
LOCUS       DAOQYN010000301         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_88573_length_1672_cov_5.277675, whole genome shotgun sequence.
ACCESSION   DAOQYN010000301 DAOQYN010000000
VERSION     DAOQYN010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88573_length_1672_cov_5.277675"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1672
                     /locus_tag="PKN80_09080"
     CDS             <1..>1672
                     /locus_tag="PKN80_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36199.1"
                     /translation="TTEANKVPKFAGEIVELVAEDTPPARSGNYLENGGFEAGLTGWG
                     RWYGSATPVLRPANLDTGGPAEGRYALRLEVPANDRRFGVETKLYRVPAGRDYTLSFA
                     ARADRPLALRAYLNGISDDLKNYSSAGLTAAVSLTTEWQRFTVSGRLLSRPGFLYTLV
                     IQPQGDERPAVVWLDAVQLQEGAAAPFQPRTPREVGWLSATPGNVFYEREPARVELLV
                     SGGGGNAAVDYQTVDFWGQVVDRGRQEVAGGRAAFNLYAGKRGLFRTTFTLGEALSEL
                     VYSVLPANPHLKEKYPAGTLGTDIGGAVPYHLDILKRANFNWAISKTLGRWYVVEPEP
                     GKYVFNDEAIQAIDQANILLLIQPLNIGWGIQPWLKEYAQPPVNSGAVWEPAKRAFYM
                     KAWGDFIYALVSRYKPYVKHWEIENEPNAGFTPGQYAEILRVASREIRRADPQAKVVG
                     FSGGGFSRKFYDPVLKACGPENFDIVSVHFYGYADPDFASFLKETGRPGWNTEVGSTG
                     PSFFTSLPNFDSVKEKDYWQLRPGIIQANAVSDVRTYLSSMSVGGIEKY"
BASE COUNT          276 a          574 c          568 g          254 t
ORIGIN      
        1 accaccgagg ccaacaaggt gcccaagttc gccggcgaga tcgtggagct ggtggccgag
       61 gacacgccgc cggcccggag cggcaactac cttgagaacg gcggcttcga ggccggcctg
      121 accggctggg gccgttggta cggttcggcc acgccggtgc tgcggccggc caacctggac
      181 accggcggcc cggccgaggg tcgttatgcg ctccggctgg aggtgccggc caacgaccgc
      241 cggttcgggg tggagaccaa gctctaccgg gtcccggccg gccgggatta cacgctgagc
      301 ttcgcggccc gggccgaccg gccgctggcc ctgcgggcct acctcaacgg catttcggac
      361 gatctcaaaa actattcatc ggccgggctc acggccgcgg tcagcctgac gaccgagtgg
      421 cagcgcttca ccgtcagcgg ccggctgctc agccggcccg gcttcctcta cacgctggtc
      481 atccagcccc agggcgacga gcggccggcg gtggtctggc tggacgcggt ccagctgcag
      541 gagggggcgg cggccccctt ccagccgcgc acgccccggg aggtgggctg gctctcggcc
      601 acgccgggca acgtcttcta cgagcgggag ccggcccggg tggagctgct ggtttccggc
      661 ggcggcggta atgccgcggt cgattaccag acggtggact tctggggcca ggtggtggac
      721 cgcggccgcc aggaggtcgc cggcggccgg gccgccttca acctttacgc gggcaagcgc
      781 ggcctcttcc ggaccacctt caccctgggc gaggccctct cggagctggt ctactcggtc
      841 ctgccggcca atccccacct gaaggagaag tacccggccg gcaccctggg caccgacatc
      901 ggcggcgccg tcccctacca cctggacatc ctgaagcggg ccaacttcaa ctgggccatt
      961 tcgaaaacac ttgggcgctg gtacgtggtg gagccggaac cgggaaaata cgtcttcaac
     1021 gacgaggcga tccaggcgat cgaccaggcc aacatcctcc tgctcatcca gccgcttaat
     1081 atcggctggg ggatccagcc ctggctgaag gagtacgccc agccgccggt caacagcggc
     1141 gccgtctggg aaccggcgaa gcgggccttt tacatgaagg cctggggcga cttcatctac
     1201 gccctggtct cccgctacaa gccctacgtc aagcactggg agattgaaaa cgaacccaat
     1261 gccggtttca ccccggggca gtatgccgaa atcctgaggg tggccagccg ggagatccgg
     1321 cgggccgacc cgcaggcgaa ggtggtcggc ttctccggcg gcggcttcag ccggaagttc
     1381 tacgacccgg tgctcaaggc ctgcgggccg gagaacttcg acatcgtctc ggtccacttc
     1441 tacggctacg ccgacccgga cttcgccagc ttcctgaagg agaccggccg gcccggctgg
     1501 aacaccgagg tcggctcgac cggccccagc ttcttcacca gcctgcccaa cttcgactcg
     1561 gtcaaggaga aggattattg gcagctgcga ccggggatca tccaggccaa cgcggtgagc
     1621 gacgtgcgga cctacctgag ctcgatgtcg gtcggcggca tcgagaagta ct
//
LOCUS       DAOQYN010000302         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_89127_length_1666_cov_2.787089, whole genome shotgun sequence.
ACCESSION   DAOQYN010000302 DAOQYN010000000
VERSION     DAOQYN010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89127_length_1666_cov_2.787089"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="PKN80_09085"
     CDS             <1..122
                     /locus_tag="PKN80_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNQ36200.1"
                     /translation="IASAIQCEKQLKKWSRAWKIRLIKDKNPGWKDLYPELTL"
     gene            430..1068
                     /locus_tag="PKN80_09090"
     CDS             430..1068
                     /locus_tag="PKN80_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNQ36201.1"
                     /translation="MKSLLGLAFAVALILSVQPAFGQQPAQPEAVKLVKPQTTGGKPL
                     MQALNERRSQRAFSSKELPPQVLSNLLWAAFGVNRPDGRRTAPSASNRQEIDIYVAMA
                     RGLYLYNAKEHRLDPVLAEDIRTVTGAERLVKDTPVVLVFVADYARMSGNKDLYSATD
                     TGYISQNVYLYCASEGLATVVLGTVNREALAGWMKLRPDQKIILTQPVGYPK"
     gene            complement(1147..1347)
                     /locus_tag="PKN80_09095"
     CDS             complement(1147..1347)
                     /locus_tag="PKN80_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNQ36202.1"
                     /translation="MTGKIKKLVRDRGFGFIASTDGTEVFFHRTSIADGNFDALNEDQ
                     SVEFDVEKSEKGPRAVNVKVVA"
     gene            complement(1475..>1666)
                     /locus_tag="PKN80_09100"
     CDS             complement(1475..>1666)
                     /locus_tag="PKN80_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36203.1"
                     /translation="AVKIRAEAPVAHIQLALSQGREGWGYLNFYGGPGKTPYPRFHEL
                     KPADRKRRVEKWWEEFKTG"
BASE COUNT          335 a          506 c          459 g          366 t
ORIGIN      
        1 atattgcgtc cgctattcaa tgcgaaaagc agctgaagaa atggtcgcgg gcctggaaga
       61 tacgcttaat taaagataaa aacccggggt ggaaggacct ttacccggag ctaacgttgt
      121 agtggtttcg tttttattca acgtttgtca ttcccgcggc tggtaagcgg gcatgtccga
      181 cgaagctcat attctgagcg taggcgggaa tccaactttt gtctgtcatt ccctcgtgta
      241 gtaggaggga atccatcgtt tgcctttttg tctgtcattc ccgaatgggt ttatcgggaa
      301 tccagggctc cttgaaatac cctcaactgt ttttaaaggt ctggataccc gcctacgaca
      361 tgcgggtata acggacaaca aaagggacag gcatgcgggt atgacgaact ttttccaaag
      421 gaggggttta tgaagagtct gctcggattg gccttcgcgg tcgctttaat tctctccgtt
      481 cagccggcct tcggccagca gccggcccag ccggaggcgg tcaagctcgt caagccgcag
      541 accaccggcg gcaagccgct catgcaggcg ctcaacgagc gccggagcca gcgcgccttc
      601 agcagcaagg agctcccgcc ccaggtcctc tccaacctgc tctgggccgc cttcggcgtc
      661 aaccggcccg acggccggcg caccgcgccc tcggccagca accggcagga gatcgacatc
      721 tacgtggcca tggcccgggg gctttacctc tacaacgcga aggagcatcg gctcgacccg
      781 gtgttggccg aggatatccg gaccgtcacc ggcgccgagc gcctggtcaa ggataccccg
      841 gttgtcctgg ttttcgtggc cgattatgcc cggatgtcag gcaacaagga cctctactca
      901 gccaccgaca ccggctatat cagccagaat gtttatcttt actgcgcttc ggaagggctg
      961 gcgacggttg tcctggggac agtaaaccgg gaggcgctgg ccggttggat gaaactgcgg
     1021 cccgaccaga agatcatcct cactcagccg gtcggctacc cgaaataata accgaccggc
     1081 tgccaggatg tttatcagac ccgggttgag gtgggctgaa taacgaggtg tggtagtggg
     1141 tttttcttac gcgacgacct ttacgttaac ggcccgcggt cccttctccg acttctcgac
     1201 atcgaactca accgactggt cttcgttcag ggcgtcgaaa ttgccatcag cgatgctggt
     1261 ccgatgaaag aaaacctcgg tcccatccgt gctggcgata aacccaaaac cccgatcccg
     1321 caccaacttc ttaatctttc ccgtcacaac tacctccttg gttgaactgc taccgcactg
     1381 cgctttactg ccgggccggc gaccagcatg gccgccttcc ctgagattta ctattatacc
     1441 cgttttcaag gtcaatagca atagctgacc ctttttaccc ggttttgaac tcttcccacc
     1501 acttctccac ccgccgcttc cggtcggccg gtttcagctc gtggaagcgc gggtaaggcg
     1561 ttttgccggg cccgccgtag aagttcaggt agccccagcc ctcacgcccc tgcgacaggg
     1621 ccagctggat atgggccacc ggcgcctccg cgcggatctt gaccgc
//
LOCUS       DAOQYN010000303         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_89838_length_1657_cov_4.785893, whole genome shotgun sequence.
ACCESSION   DAOQYN010000303 DAOQYN010000000
VERSION     DAOQYN010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89838_length_1657_cov_4.785893"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKN80_09105"
     CDS             <1..298
                     /locus_tag="PKN80_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNQ36204.1"
                     /translation="DDVIFEEFKGTGNMEVNLDRHLAERRIWPAIDINKSGTRREELL
                     LDPDELKRVWILRKILASLPPEEAMGLLLDKLHKTGSNTEFLLSIRTEEASSLR"
     gene            313..1269
                     /locus_tag="PKN80_09110"
     CDS             313..1269
                     /locus_tag="PKN80_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ36205.1"
                     /translation="MSRLKVSGLRVAFRTPGGRQEILRGLDLEVRDGEIVGLCGESGA
                     GKSLLVLSVLGLLPPGAEFVAGRIEFEGRELTGHPDRAARLRGRELAVVFQDPTASLD
                     PLYRAGNQVAEALAALGRPAGRSEVVRCLAATDLKEPDRLAWHYPHQLSGGEKQRVVL
                     AISLAGRPRLLLADEPTASLDSATSDQVTGLLNRIRREQGTSILFISHNLPLVAGLAD
                     RIAIIYAGTLLEIGPARSLVERPLHPYTRLLYAALPDPGRRGQPLATVIGRPGQPSEA
                     DRGCVFFERCPDRRAACAAERPVIKEIFPDHRVACFRAGQPD"
     gene            1294..>1657
                     /locus_tag="PKN80_09115"
     CDS             1294..>1657
                     /locus_tag="PKN80_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36206.1"
                     /translation="MPNRVYDYLLAALFLLLSLLTMRVAYDYTLIRLVVVNLFVATAA
                     AGFLAARRPMRLPLTVLIPLAAFLLFTTVSIAFSYFPAATLREVFHLVTFVGLFLVGL
                     QLNLGWPGLVAWLAGAVIL"
BASE COUNT          237 a          548 c          539 g          333 t
ORIGIN      
        1 ggatgacgtt atctttgaag aattcaaggg caccggcaac atggaggtca acctggaccg
       61 gcacctggct gaacggcgca tctggccggc catcgatatc aacaaaagcg gcacccggcg
      121 ggaagaactc ctgcttgacc cggacgaact caagcgggtc tggatcctgc gtaaaatcct
      181 ggcctcgctg cctccggagg aggcaatggg tctcctgctg gacaagcttc acaagacggg
      241 ttccaacacc gagtttctgc tttccatccg taccgaagag gccagttcgc tgcgctgaag
      301 gcctttatcc cgatgagccg gctcaaggta tccggtctgc gggtggcgtt tcggacgccc
      361 ggcgggcgcc aggagatcct gcgcggactg gacctggagg tccgggacgg tgaaatcgtg
      421 ggcctttgcg gcgaatccgg cgccggcaag tccctgctgg ttctgtccgt tctggggctg
      481 ctgccgcccg gcgccgaatt cgtggctggt cggattgaat tcgaaggccg cgagttgacc
      541 ggccacccgg accgggccgc ccggctgcgc ggccgcgagc tggcggttgt cttccaggat
      601 ccgaccgctt cgcttgaccc gctttaccgg gccgggaacc aggtggcgga ggcactggcc
      661 gcgctgggcc ggccggccgg ccgcagtgag gtggtgcgct gcctggccgc caccgacctg
      721 aaggagccgg atcggttggc ctggcattac ccgcaccagc tttcgggcgg cgaaaaacag
      781 cgggtggtcc tggccatcag cctggccggg cgccccaggc tcctgctggc cgacgagccg
      841 accgcctcgc ttgattcggc cacctccgac caggtgaccg gtcttttgaa ccggatccgg
      901 cgcgagcagg ggacctcaat ccttttcatc agtcacaacc tgccgctggt ggccggcctg
      961 gctgatcgga tcgccatcat ttacgccggg acgctgctgg aaatcgggcc ggcccgttcc
     1021 ctggtcgaac ggccgctgca tccctatacc cgactgttgt acgcggctct gccggatccg
     1081 ggccgccggg gtcaaccgct ggccaccgtc atcggccggc ccggccagcc gtccgaagcc
     1141 gaccggggct gcgttttttt cgagcgctgt ccggaccggc gggcggcctg cgcggccgaa
     1201 cggccggtta tcaaggaaat atttcccgac catcgggtgg cctgcttccg tgccggccag
     1261 ccggattgaa aaacccttca agcgaggtgg gtgatgccga accgcgttta cgattacctt
     1321 ctggcggccc tgttcttgtt gctttccctc cttaccatga gggtcgccta cgattacacc
     1381 ctgatccggc tggtggtcgt caaccttttc gtggcgacgg ccgcggccgg tttcctggcg
     1441 gcccggcgtc cgatgcgcct gccgctgacc gttttgatac ccctggcggc cttcctgctc
     1501 ttcaccacgg tttcgatcgc cttttcctat tttccggcgg ccacgctcag ggaggtcttc
     1561 cacctggtta ccttcgtcgg tctcttcctg gtgggacttc agctgaatct gggctggccc
     1621 gggctggttg cctggctggc cggcgcggtg atcctct
//
LOCUS       DAOQYN010000304         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_90652_length_1648_cov_4.367232, whole genome shotgun sequence.
ACCESSION   DAOQYN010000304 DAOQYN010000000
VERSION     DAOQYN010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90652_length_1648_cov_4.367232"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PKN80_09120"
     CDS             <1..643
                     /locus_tag="PKN80_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNQ36207.1"
                     /translation="VDSESLRRDDELERSLAGVDGILVPGGFGTRGVEGMVAAIRYAR
                     EKRIPFFGICLGLQVAVIEFARNVCGLDRANSTEFDPQTPHPVITLMDSQRQVQAMGG
                     TMRLGGYPCVLAAGSLSRRCYGVPRIVERHRHRYEVNPHLFGRLQEKGLQASGRSPDR
                     KLVEIVELKDHPFFVATQFHPEFQSRLASPHPIFSAFIAAALRQRLENRPPSA"
     gene            664..>1648
                     /locus_tag="PKN80_09125"
     CDS             664..>1648
                     /locus_tag="PKN80_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ36208.1"
                     /translation="MKDILNVGFIGCGGFVQGSHLPNAAANPKLRVRALCDLQADRLK
                     ALEPVYRPDYVTTDLTRLAADPELDLVVIGTGPKVRVEPIRAAAEAGKHIYVEKPMSL
                     GYEDSLEIVRIIRQTGVKLQVGFNRNYSPIMQEARRVYRKMKRGETLISYRIVGEAQL
                     WPKFHQEAVKGGESTIIHELTHIFDLINWLTDSDPRSVFVIGGPSDNNILAMEYPDDV
                     YVTLLSGSCGTEAYPKERMEVFSDHAVLVMDGFMDLECVRIPGESDRHYPLKANPFGP
                     LDIPLAGHKADLRRWRDSLTPAQIEKGYYYDNRPGVGKGHYEALEFLRRCVV"
BASE COUNT          289 a          561 c          525 g          273 t
ORIGIN      
        1 ggtcgattcc gaaagcctgc gccgcgacga tgaactggaa cgaagcctgg ccggggtgga
       61 cggcatcctg gtgcccggcg gcttcggcac ccggggcgtg gaggggatgg tggccgccat
      121 ccgttacgcc cgggagaagc gcatcccgtt cttcggaatc tgcctgggat tgcaggtggc
      181 ggtgatcgag ttcgcccgca acgtctgcgg tctggaccgg gccaacagca ccgagttcga
      241 cccccagacc ccgcacccgg tgattaccct gatggactcc cagcgccagg tccaggcgat
      301 gggcggcacc atgcggctgg gcggttatcc gtgcgtcctg gcggcggggt cgctgagccg
      361 ccgctgctac ggggtgcccc gcatcgtgga acgccaccgc caccgctacg aggtcaaccc
      421 gcacctcttc ggccgcctcc aggagaaggg actgcaggcc tcgggccgct ccccggaccg
      481 gaaactggtc gagatcgtgg aattgaagga tcaccccttt ttcgtggcca cccagttcca
      541 tcccgagttc cagtcgcgcc tggcttcacc gcacccgatt ttcagcgcct tcatcgccgc
      601 cgcgctcagg cagcggcttg aaaacaggcc gccgtccgcc tgaaacaccg gaggaaaaga
      661 cccgtgaagg atatcctgaa cgtcggcttt atcggctgcg gcggttttgt ccagggcagc
      721 cacctgccca acgccgccgc caacccgaaa ctgcgcgtcc gggcgctctg cgacctccag
      781 gccgaccgcc tcaaggcgct ggagccggtc taccggcccg attacgtcac caccgatttg
      841 acgcggctgg ccgccgaccc cgaactggac ctggtggtca tcggcaccgg tcccaaggtc
      901 agggtggaac cgattcgggc ggccgccgag gccggcaagc acatctacgt cgagaagccg
      961 atgagcctgg gctacgagga ctcactcgaa atcgtccgga tcatccggca gaccggggtg
     1021 aagctccagg tgggcttcaa ccgcaactat tcgcccatca tgcaggaggc ccggcgcgtc
     1081 taccggaaga tgaagcgggg cgagacgctc atctcctacc ggatcgtcgg cgaggcccag
     1141 ctctggccga agttccacca ggaggcggtc aagggcggcg aatcgaccat catccacgag
     1201 ttgacccaca tcttcgacct gatcaactgg ctgaccgatt ccgacccccg gagcgtcttc
     1261 gtgatcggcg gtccttccga taacaacatt ctggccatgg agtatccgga cgacgtctac
     1321 gttaccctgc tctccggcag ctgcggtacc gaggcctatc ccaaggagcg gatggaggtc
     1381 ttctccgatc acgcggtgct ggtcatggac gggttcatgg acctggagtg cgtgcggatt
     1441 cccggggagt cggaccggca ttaccccctg aaggccaacc cgttcggtcc gctcgacatt
     1501 ccgctggccg ggcacaaggc cgacctccgg cgctggcgcg attcgctcac cccggcccag
     1561 atcgaaaagg gctactacta cgacaaccgg ccgggcgttg gcaagggcca ttacgaggca
     1621 ctcgagttcc tgcgccgctg cgtggtcg
//
LOCUS       DAOQYN010000305         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_92095_length_1633_cov_2.425222, whole genome shotgun sequence.
ACCESSION   DAOQYN010000305 DAOQYN010000000
VERSION     DAOQYN010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92095_length_1633_cov_2.425222"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1633)
                     /locus_tag="PKN80_09130"
     CDS             complement(<1..>1633)
                     /locus_tag="PKN80_09130"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNQ36209.1"
                     /translation="DPAAGTLTFPAEAAKLAVEEFKGKYGTGNRERLIREHILKNFYA
                     FELMMAPYAIGHIKISFLLEELGYALKGDDRFNLYLTNTLEMEEIHATNLPGMASLSE
                     ESEAAGKVKKQIPILVIMGNPPYSGHSSNKYEEVKEYYQVDGKPLKEKNPKWLQDDYV
                     KFIRFSQWKIDQAGEGVLGFITNHGYLDNPTFRGMRQSLLKSFDEIYLLDLHGNSKKK
                     ETSPDGSKDENVFDNQQGVAIGLFIKKKGDGVKGDAKVYHADLYGRREEKYRWLEEKD
                     IHTTDWRQLTPHSETYFFMPRDEKLLGRYEKHPKIPDIFLVNSVGIVTARDNLTIQWT
                     PEQIRKIIMLFPRMDSESARTTYQLGKDARDWKVELAQRDLLESGMDNSRIQPILYRP
                     FDIRYTYYTGKSRGFLCMPRPEVMRHMLRENLGLITRRQMLPNHPCNYVFISNKLISD
                     GVIRSDNKGSESLFPLYLYPEKGEPHIFSSTDSQKEREPNLNSAIVGRLSETYGRQPL
                     PEEVLYYVYAVLYAPAYRSRYAEFLKSDFPRIPFTG"
BASE COUNT          354 a          418 c          438 g          423 t
ORIGIN      
        1 tccagtaaat ggtatccggg gaaaatcgct cttcaggaat tccgcgtacc ggctccggta
       61 ggcgggcgcg tagaggacgg cgtaaacata ataaagaacc tcttccggaa gtggttgccg
      121 gccgtaggtc tcggatagac ggccgacgat ggccgaatta aggttgggct cccgctcttt
      181 ttggctatcg gtcgagctga atatgtgggg ttcgcctttc tccgggtaaa gataaagtgg
      241 gaataaagat tcgctaccct tattatccga tcttatcacc ccatccgaaa ttaacttgtt
      301 ggaaataaaa acataattac acggatggtt gggtaacatt tgtcgtcgcg tgatcaaccc
      361 caggttttcc cgcagcatgt gacgcattac ttccggccgg ggcatgcaga gaaaaccgcg
      421 cgattttccg gtgtagtaag tatatcgaat atcaaacggc cggtataata tcggctgtat
      481 tctggagttg tccataccgc tttccagcag gtcccgctgg gcaagttcca ccttccaatc
      541 ccgggcatct ttacccaatt gataggttgt ccgagccgac tcggaatcca tcctgggaaa
      601 gagcatgatt attttacgga tttgttccgg cgtccattga atggtcagat tatcccgggc
      661 cgtaacaatg ccgacggagt taaccagaaa aatatccggg attttaggat gtttttcata
      721 tctgcccagc agtttctcat cccggggcat aaaaaaatag gtctcagaat ggggcgtcaa
      781 ttggcgccag tccgtagtgt ggatatcctt ctcttccagc caccggtact tctcctcgcg
      841 tcggccgtaa aggtcggcgt ggtaaacctt agcgtcgcct ttcacgccat cccccttctt
      901 tttaatgaag aggccgatgg ctacgccctg ttggttgtca aagacattct cgtccttgga
      961 accgtccgga gaggtttcct tcttcttgga gttgccgtgc agatcgagca ggtaaatctc
     1021 gtcaaaactc ttcagcaggg actggcgcat gccccggaag gtcgggttgt cgaggtaacc
     1081 gtggttggta atgaaaccga ggacgccttc gccggcttgg tcaatcttcc actggctgaa
     1141 gcggatgaac ttgacataat catcctggag ccacttcgga ttcttttcct tgagcggttt
     1201 cccgtcaacc tggtaatact ccttaacctc ctcgtacttg ttggaggagt ggccggaata
     1261 gggcggattg cccatgatga ccaggatcgg gatttgcttc ttgacctttc ccgccgcctc
     1321 cgactcctca gagagggagg ccatgcccgg cagattggtg gcgtggatct cctccatctc
     1381 gagggtgttg gtcaggtaaa ggttaaaacg atcatctccc ttcaaggcat atcccagttc
     1441 ttccagcaga aaggatattt tgatgtggcc gatggcgtaa ggggccatca tcaattcaaa
     1501 agcgtagaag ttcttgagga tatgctcccg gatcaggcgt tcccggttgc cggttccgta
     1561 ttttcctttg aactcctcca cggccagttt cgccgcctcg gcgggaaagg tcagggtgcc
     1621 ggcggccgga tcg
//
LOCUS       DAOQYN010000306         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_92520_length_1628_cov_3.735537, whole genome shotgun sequence.
ACCESSION   DAOQYN010000306 DAOQYN010000000
VERSION     DAOQYN010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92520_length_1628_cov_3.735537"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PKN80_09135"
     CDS             complement(<1..269)
                     /locus_tag="PKN80_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNQ36210.1"
                     /translation="MLWTNYLLPTQKEVRIEGEDTASLRLTLQAGLIRQLASGIYTFL
                     PFGLAVLRRIEDVVREEMNRAGAVELLMPALQTTSLWKESDRYQLL"
     gene            complement(295..1260)
                     /locus_tag="PKN80_09140"
     CDS             complement(295..1260)
                     /locus_tag="PKN80_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNQ36211.1"
                     /translation="MKLGVLLVLFGQKTLEEALDYAKKCGVEAVEIGTGNYPGNAHCR
                     PEELLANPRRIKEFKKAIEDRGLILSALSCHGNPLHPDSKIAARHREVQRQTIRLAEK
                     LGLDTIITFSGCPAGGPDDRTPNWITCPWPEDYSRSLAWQWEKQAVPYWRKEAAFAAD
                     HGVKKIALEMHPGFLVYNPETLLKLRTAVGPAIGANFDPSHLFWQGIDPVRAVTALEG
                     AVFHCHAKDTRIDHYNTALNGVLDTKPYGNEKERSWIFRTVGYGHDQAFWKDFISALR
                     LSGYDGVLSIEHEDSLLSGNEGFLKAVRFLKEVIVSEPRGAMWWA"
     gene            complement(1440..>1628)
                     /locus_tag="PKN80_09145"
     CDS             complement(1440..>1628)
                     /locus_tag="PKN80_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36212.1"
                     /translation="TKTELLIFLTPHVAKQPGDLQEFSAGTLEGTSIVNKAVTPGAFE
                     KHLKGMERGGPAAPAVNP"
BASE COUNT          280 a          471 c          520 g          357 t
ORIGIN      
        1 aggagttggt aacggtcgga ctccttccag agcgaggtcg tctgcagggc cggcatcagg
       61 agttccacgg ccccggcgcg gttcatctcc tcccggacga cgtcttcgat ccggcgcagg
      121 acggccaggc caaaaggcag gaaggtgtaa attccgctgg ccagctgacg gatcaggccg
      181 gcctgaagcg tgagccgcag gctggcggta tcttcgccct cgatgcggac ttctttctgg
      241 gtcggcagca ggtaatttgt ccagagcatg gtttccgttt ggtttggccc gtggtcaggc
      301 ccaccacatg gcgccgcggg gttcggagac aatgacttcc ttcagaaaac ggacggcctt
      361 caggaatccc tcgtttccgg aaagaaggct gtcctcgtgt tcaatggaga gaactccgtc
      421 gtaaccggac agccggaggg cgctgatgaa atccttccag aaggcctggt catgtccgta
      481 gccgacggtg cggaagatcc agctgcgttc cttttcgttt ccgtacggct tggtgtcaag
      541 cactccattg agggccgtgt tgtaatggtc aatccgggtg tccttggcgt ggcagtggaa
      601 gacggcgcct tccagggccg tcaccgcccg aaccggatca attccctgcc agaaaaggtg
      661 gctggggtcg aagttggcgc cgatggccgg ccccaccgcc gttcgaagtt tcagcagggt
      721 ttccggatta tagaccagaa agcccggatg catttccagg gcgatcttct tcactccgtg
      781 gtcggcggcg aaggccgcct ccttccgcca gtagggaacc gcctgttttt cccactgcca
      841 ggccaggctt cggctgtaat cctcgggcca ggggcaggtg atccagttcg gggtccggtc
      901 atccggtccg ccggccgggc agccggagaa ggtgatgatg gtgtccaggc cgagtttttc
      961 cgccagtcgg atggtttgac gctgaacctc ccggtgccgg gcggcgattt tggagtcggg
     1021 gtgaagcgga tttccgtgac aggaaagggc cgacaggatc aggccccggt cctcgatcgc
     1081 cttcttgaac tccttgatcc ggcggggatt ggccagcagc tcttccggcc gacagtgtgc
     1141 gttcccggga taattaccgg ttccgatctc gaccgcttcc accccgcatt tcttagcgta
     1201 atcaagcgcc tcttcaagcg ttttttggcc gaagagcacc agcaataccc cgagtttcat
     1261 ctttatcctc ccggattacg gagaccaagg gtttgggaca agagttataa cgtctgcctt
     1321 cgggaataag gtgagtgacc cgcttagatt ttacttcttt cccaacggcg cggtcaattc
     1381 tttccccggt cggccggatt cgagacgatc gaccgggaaa cggcctggtt cgccaccggt
     1441 cagggattga cggccggagc cgccgggccg cccctttcca tgcccttgag gtgtttttcg
     1501 aaggcgcccg gggtgacggc cttgttgaca atgctggttc cttccagcgt gccggcggag
     1561 aattcctgga ggtcgccggg ctgcttggcc acgtgcgggg tcaggaagat caggagttcg
     1621 gtcttggt
//
LOCUS       DAOQYN010000307         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_92839_length_1625_cov_2.749045, whole genome shotgun sequence.
ACCESSION   DAOQYN010000307 DAOQYN010000000
VERSION     DAOQYN010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92839_length_1625_cov_2.749045"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PKN80_09150"
     CDS             complement(<1..368)
                     /locus_tag="PKN80_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ36213.1"
                     /translation="MKKLLLTVLVIAAACLLVGLKTASVSAQTSAEEYFVAGVEFFEG
                     KNYTAAATAFEKAVELKPDYEDARFNLAVTYWTQKQYQKAAATLEELIQRNPESEVGK
                     QAAQELDKLKKSGIFAKEEE"
     gene            complement(399..719)
                     /gene="trxA"
                     /locus_tag="PKN80_09155"
     CDS             complement(399..719)
                     /gene="trxA"
                     /locus_tag="PKN80_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNQ36214.1"
                     /translation="MEIILDENNFEQEVKEGGLPVLVDFWAAWCGPCQMLGPVITQVA
                     EEYQGKVKVGKVNVDENPNLAGKFGIMGIPTMILFINGEEKERLVGVAPKERITKLLD
                     ALKQ"
     gene            complement(771..1304)
                     /locus_tag="PKN80_09160"
     CDS             complement(771..1304)
                     /locus_tag="PKN80_09160"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNQ36215.1"
                     /translation="MTNRKRWAGGGIPLAVFAALIWFGALFIPVVQGLDAGSKAPDFK
                     TVTIDGKALELKNFKGKVVVLNFWATWCPPCRAEIPDFIEFTREYGKKVQVIGIGLER
                     KPGNTTQILRRFVDQNKINYPVVDDSEGKLTVLYGNIRSIPTTFIIDASGIVRHMQIG
                     SMSKAELIGKVKPLLKK"
     gene            complement(1328..1549)
                     /locus_tag="PKN80_09165"
     CDS             complement(1328..1549)
                     /locus_tag="PKN80_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36216.1"
                     /translation="MPGLTEETDLLEIARFLRERAGTLPVRYAVQNFFPAETMDASFR
                     GARPWEKERLERVRLELSPFFKEVIIRSR"
BASE COUNT          346 a          512 c          378 g          389 t
ORIGIN      
        1 tcctcttcct ccttggcgaa gataccgctt ttcttcaact tgtcgagttc ctgggccgcc
       61 tgctttccaa cttcgctctc cggatttcgc tgaatcaatt cctccagagt ggcggccgcc
      121 ttctggtatt gtttctgcgt ccagtaagtg accgccaggt tgaagcgggc atcctcgtaa
      181 tcgggtttca attcgaccgc tttttcaaag gcggtcgccg ccgccgtata attttttccc
      241 tcaaaaaact cgaccccggc gacaaaatac tcctcggcgc tggtctgggc gctaacggag
      301 gcggttttca aaccaaccag caggcaggcc gctgcaataa ccagcaccgt caggagtaat
      361 tttttcatgg atctatcagt cctaagcctt gttttaaact attgtttcag ggcgtccagc
      421 agcttggtga ttctttcctt gggcgccaca ccgaccaggc gctccttctc ttcaccgttg
      481 ataaaaagaa tcatggtggg tattcccatg atgccgaatt taccggccag attgggattc
      541 tcatccacgt tgaccttgcc cacctttacc ttcccctgat actcctccgc cacctgggtg
      601 ataaccggcc ccagcatctg gcacgggccg caccaggccg cccagaaatc caccagcacc
      661 gggaggccac cctcctttac ctcctgttcg aaattattct catccaggat tatttccata
      721 atcggatccc cactgttttt aatgacagat cgggtcggct tcacccgacc ctatttcttc
      781 aaaagcggtt tcacttttcc gatcaactcc gccttgctca tcgatccaat ctgcatatgg
      841 cgcacaatcc cggaagcgtc gatgatgaag gtggtcggga tactgcggat gttgccgtag
      901 agaacggtca acttaccctc ggaatcatcc accaccgggt aattgatttt attctggtcg
      961 acaaaccggc gcaggatctg cgtcgtgttg ccaggcttac gctccagacc aataccaatg
     1021 acctgaactt ttttcccgta ctctcgggtg aactcgatga aatccgggat ctccgcccgg
     1081 cagggcggac accaggtggc ccagaaattc agcaccacca ccttgccctt gaagttcttg
     1141 agttccaggg ccttcccgtc aatggtcacg gtcttgaaat ccggagcctt gctgccggcg
     1201 tcaagcccct ggacaaccgg gatgaaaagg gccccgaacc aaatcagcgc cgcaaacacg
     1261 gccagcggga taccgccgcc ggcccatctt ttccgattcg tcatcagccg cctcacttcc
     1321 gtgctcgtca acgactgcga atgatcactt ccttgaaaaa cgggctcaat tccaggcgca
     1381 cccgctccag gcgctccttc tcccaggggc gagccccgcg gaaggaagcg tccatggtct
     1441 cggccggaaa aaagttctgg accgcgtaac ggaccgggag cgtacccgct cgttcccgca
     1501 gaaaacgggc tatctcaagc aggtccgttt cctccgtcaa ccccggcata atcgtggtac
     1561 gtaattcata ttctaacctt gagtttaaaa ttaagtcaac agaagacccg atccgttcca
     1621 tcagc
//
LOCUS       DAOQYN010000308         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_95249_length_1600_cov_3.979935, whole genome shotgun sequence.
ACCESSION   DAOQYN010000308 DAOQYN010000000
VERSION     DAOQYN010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95249_length_1600_cov_3.979935"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PKN80_09170"
     CDS             <1..499
                     /locus_tag="PKN80_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNQ36217.1"
                     /translation="ALAAELDGDVYLNLQADEPLIAPEVIDAVARSFDDPSVQMATAV
                     TPLTRPEDWEDTNAVKTVLDRNSDCLYFSRAAIPYDRDNRGRLPESSVYKHLGIYGYR
                     RKFLQEFAAWPPTPLEKIERLEQLRALEHGVRLRAVVTPFDSPSVDTPEDLARVAAIL
                     KSQKN"
     gene            499..1221
                     /gene="uppS"
                     /locus_tag="PKN80_09175"
     CDS             499..1221
                     /gene="uppS"
                     /locus_tag="PKN80_09175"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018302447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNQ36218.1"
                     /translation="MHVAIIMDGNGRWAQARGLPRVMGHRRGAERLDEVVEACESLGV
                     KYLTIFAFSTENWRRPKPEVSFLLSLLVDQLRRKIEKMKARGVRIRFLGDRSAFGPEI
                     RSVLEESESRTSRNRKVNLSVCLNYGGQDEILRAVRAACVDAREGRLDPAELDRERFS
                     AYLDTRDLPPVDLVIRTSGEQRLSNFLLYQSAYAEFYFTSCLWPDFDIEEFKKALAEF
                     ASRQRRFGDTASRSGPPPENNQ"
     gene            1218..>1600
                     /locus_tag="PKN80_09180"
     CDS             1218..>1600
                     /locus_tag="PKN80_09180"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.2"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNQ36219.1"
                     /translation="MKRVVIFGATGSIGNSTLAVIRRFPDDFRVVGLSGHCRHDLLQK
                     QVREFRPEAVAFSGPAPESGFQAPGARVLTGPGGLDQLADWPGVDLFVLAIPGSDALP
                     IALRILESGRPLALASKEAMVMAGEL"
BASE COUNT          287 a          543 c          491 g          279 t
ORIGIN      
        1 cgccctggcc gccgaactgg atggagacgt ttacctgaac ctccaggccg acgagccgct
       61 gatcgcaccc gaagtgatcg acgcggtggc cagatccttt gacgatccct cggtgcagat
      121 ggccacggcg gtaaccccgc tgaccaggcc cgaggactgg gaggatacca acgccgtcaa
      181 aaccgtcctg gaccggaact ctgattgcct ctacttttcc cgggcggcca taccttacga
      241 ccgcgacaac cggggacggc tcccggagag cagcgtctac aagcacctgg gcatctacgg
      301 ttaccggcgg aaattcctgc aggaattcgc cgcctggccg ccgacgccgc tggaaaaaat
      361 cgagcgcctg gagcagttgc gcgccctgga acacggcgtg cgactccgcg cggtggtgac
      421 gcccttcgac agtccttcag tggacacccc cgaagacctg gcccgggtgg cggccattct
      481 caagagtcaa aaaaactgat gcacgttgcc atcatcatgg acgggaacgg ccgctgggcc
      541 caggcccgcg gtctgccccg ggtgatgggc caccgccgcg gcgccgaacg cctggacgaa
      601 gtggtggagg cctgcgaatc gctcggggtg aaatacctga ccatcttcgc cttctccacc
      661 gagaactggc gccgacccaa gccggaggtt tccttcctgc tctcgctcct ggtcgaccag
      721 ttgcggcgca aaattgaaaa gatgaaggcc cggggcgtgc gcatccgttt cctgggcgac
      781 cgttccgcct tcggaccgga aatccgctcg gttctcgagg agagcgaaag ccgtaccagc
      841 cgaaaccgca aggtcaacct ttccgtctgc cttaattacg gaggacagga cgagatcctc
      901 cgggccgtcc gggccgcctg cgtcgatgcc cgggaagggc ggctcgaccc ggccgaactg
      961 gaccgggaac gcttcagtgc ctacctggac acccgggacc tgcccccggt tgacctagtc
     1021 atccggacca gcggagaaca gcgcctttca aatttcctgc tctaccagtc ggcctacgcc
     1081 gagttttatt tcacttcctg tctctggccc gacttcgaca tcgaggaatt caagaaggcg
     1141 ctggccgagt tcgcctcccg ccagcgccgt ttcggcgata ccgcgagccg ttccgggcct
     1201 ccgcctgaga ataaccaatg aaaagggtcg tcatcttcgg cgccaccggt tccatcggaa
     1261 acagcaccct ggcggttatc cggcgttttc cggatgactt ccgggtggtc ggcctctccg
     1321 gccactgccg gcatgacctt ctgcaaaaac aggtgcgcga attccggccc gaggcggtcg
     1381 ccttcagcgg cccggccccg gagtcgggtt tccaggcccc gggcgcccgg gtgctgaccg
     1441 gccccggagg cctggatcaa ctggccgatt ggccgggagt ggatcttttc gtactggcca
     1501 ttccgggctc cgatgcgctg cccatcgccc tgcggatcct ggaatcagga cgtccgctcg
     1561 ccctggccag caaggaggcc atggtcatgg ccggcgaact
//
LOCUS       DAOQYN010000309         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_96194_length_1590_cov_3.674267, whole genome shotgun sequence.
ACCESSION   DAOQYN010000309 DAOQYN010000000
VERSION     DAOQYN010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96194_length_1590_cov_3.674267"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..190
                     /locus_tag="PKN80_09185"
     tRNA            114..190
                     /locus_tag="PKN80_09185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Arg,seq:ccg)
     gene            242..1513
                     /locus_tag="PKN80_09190"
     CDS             242..1513
                     /locus_tag="PKN80_09190"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNQ36220.1"
                     /translation="MDLLIRNGRVVDPAQQIDETLDVSCSGAKILRLGKKLPVPSPRT
                     EIIDARGLVVTPGWIDAHCHLREPGREDEESIASGAAAAASGGFTTLMAMPNTTPCID
                     NAALVAFVLNEGARTPVRVLPIGAITRDRAGKELTEMAEMAERGAVAFSDDGNCLADS
                     RILRRALEYSRLAGRPLIDHPEDPALSRNGAMNEGTLSTRLGLAGIPREAEEIAVSRD
                     IALAGLTGGPLHLAHLTTAGSVELVRQGKKKGLPVTAEVTVHHLTLTEAAVSGYDTNA
                     KVSPPLRTAADIKALRKGLRDGTIDIIVTDHAPHSVEEKETDFQNAACGMIGLQTALP
                     LVNELAGEIPLAVLVAGLTCNPARIFGLKECGSLQPGYRADLTIYDPESSWVLKPELI
                     LSRSRNTPFLGRRLKGRAIRTVCGSRTIFKA"
BASE COUNT          306 a          571 c          477 g          236 t
ORIGIN      
        1 ctgggaccgg tacatccaga ccgccctgaa ctttcatcga cacacactaa ccgggcactc
       61 ctcccgaacc gctctttcaa agaaggttcc cggtgcagta taatatataa gaagcgcttg
      121 tagctcaact ggatagagcg ttagcctccg gagctaaagg ttcagggttc gactccctgc
      181 aagcgcgcca ggaggcccat ggcggcctcc tgccgcaagc gcggtttcac caggaccctc
      241 tatggacctt ctgatcagga acggccgggt ggttgatccg gcccagcaga ttgacgaaac
      301 cctggatgtc tcctgttccg gcgccaaaat cctccgcctg ggcaaaaaac tgcccgttcc
      361 ttccccccgt accgaaatca tcgatgcccg aggcctggtg gtaacccccg gctggatcga
      421 cgcccactgc cacctgcgtg aaccgggccg ggaggacgag gagagtatcg ccagcggggc
      481 cgccgcggcc gcgtccggtg gtttcaccac gctcatggcc atgcccaaca ccacgccctg
      541 catcgacaat gccgccctgg tcgccttcgt cctcaatgaa ggagcccgga ccccggtacg
      601 cgtgctgccc atcggagcga tcacccgtga ccgggccggc aaggaactga ccgaaatggc
      661 cgagatggcc gaacggggag cggtcgcctt ctccgacgac ggcaattgcc tggccgacag
      721 ccggatcctg cgccgggcgc tggagtatag ccggctggcc ggccggccgc tcatcgacca
      781 tcccgaagac ccggccctct cccgcaacgg cgccatgaac gaggggaccc tctcgacccg
      841 gctgggactg gccggcattc cccgggaggc cgaagagatc gccgtcagcc gggatatcgc
      901 tctggccggc ctgaccggcg gcccgcttca cctggcccac ctgaccacgg ccggctcggt
      961 cgaactggtc cgccagggga agaaaaaagg attgccggtg accgccgagg tgacggtcca
     1021 ccaccttacc ctgaccgagg cggccgtctc cggatacgac accaacgcca aggtaagccc
     1081 gcccctgcgg accgccgccg acatcaaggc gctgcgcaag ggcctgcgtg acggcaccat
     1141 cgacattatc gtgaccgacc acgcccccca cagcgttgag gagaaggaaa ccgattttca
     1201 aaacgccgcc tgcggtatga tcggcctgca gacggccctg ccgctggtaa acgagctggc
     1261 cggagagatc ccgctggcgg tcctggtggc cggactaacc tgcaacccgg cccggatctt
     1321 cgggctcaag gaatgcggca gcctgcagcc cgggtaccgg gcggacctga ccatctacga
     1381 cccggaatcc agctgggtgc tgaagccgga gctgatcctg agccgcagcc gcaacacgcc
     1441 cttcctcggc cgacgcctca aggggcgcgc catccgtacc gtctgcggca gtcggaccat
     1501 cttcaaggcc tgaaggcacg caacgtccgg agttccaccc ggccccccgg gaaccggccg
     1561 cccccccttt tcaaaccaca agaggagtcc
//
LOCUS       DAOQYN010000310         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_98071_length_1572_cov_4.437047, whole genome shotgun sequence.
ACCESSION   DAOQYN010000310 DAOQYN010000000
VERSION     DAOQYN010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98071_length_1572_cov_4.437047"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..>1572)
                     /locus_tag="PKN80_09195"
     CDS             complement(101..>1572)
                     /locus_tag="PKN80_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36221.1"
                     /translation="SGGVQFGQPYLYLPGRDRPVPVNDIHRYPGLPADREFWEFLTTA
                     GQPGRVFQVNKRWNFSLTGARNRLTFVTEIGCGDYGRLPEILKEYRRRHAWKGLNDYR
                     IFSREWEAFLTAYRQAGLEGIFRSVDEFRRLTGQAAAEGTRRLLEGLLINPYCGGYIL
                     YHYDESCWPMFGGLVTLWNQVRPEPYRALKELNADRVLVLEWGPRNFYAGRECEVTAW
                     LVDQTRTPHAGRVRLSLAAGGKSTAGELAYRSRGRRVERIGAWRFRAPETTGFGTLLG
                     QLETEAGSSTPPVKYQVFVGPAGRLAGLKADLGRLPEELRANLAGSGIKNGPGPILMD
                     AAATTPIQLQAVRNEVKQGAAAIILHPETAGCLPPGLSSRPSRGWIFPSFHFLSEHPC
                     FRDCRGDGVVGPAWAELAPRFSLQAEPASGRELIGGFLVQNYRAGLFGTSFSHGHDLV
                     IQREGKGRVIFCTYRLLETVGRNPLADTLLANILAEAGR"
BASE COUNT          259 a          530 c          522 g          261 t
ORIGIN      
        1 gcttccacca ggacccgatc taccggccgg acctctcggt ggaaaacctg gcccgtttca
       61 accgcctggc cgcctggacc ctccgcctcg cctaccgaaa ctaacggccg gcttccgcca
      121 ggatattggc cagaagcgta tcggccagcg ggttccttcc caccgtttcc agcaaccggt
      181 aggtgcagaa gataacccgc cccttgccct ctctttggat gacaagatcg tggccgtggc
      241 tgaatgaggt cccgaagaga ccggcccggt aattctggac caggaagccg ccgataagct
      301 cccgtccgga tgccggctcc gcctggaggc tgaaacgggg cgccagctcc gcccaggccg
      361 gcccgacgac cccgtctccc cggcagtccc ggaagcaggg gtgttcggac aggaagtgaa
      421 aggaggggaa gatccagccg cgcgaggggc ggctgcttaa ccccggggga agacaaccgg
      481 ccgtttccgg atgaaggatg attgccgcgg ccccttgctt gacttcgttc cggactgctt
      541 gcagttggat cggcgtcgtc gcggccgcgt ccatcaaaat cggtcccggt ccgtttttga
      601 tgccagagcc ggccaggttg gcgcgcagct cttccggcag ccggccgagg tcggccttta
      661 agccggccag ccggccggcc ggtcccacga agacctggta tttgaccggt ggcgtggaag
      721 atccggcctc ggtctccagt tggccgagca gggtgccgaa gccggtcgtt tccggggcgc
      781 ggaagcgcca ggcgccgatt ctttcgaccc ggcggccgcg cgagcggtag gccagttccc
      841 cggcggtcga tttgccgccg gcggccaggc tgagccggac ccggccggcg tggggcgtcc
      901 gggtctggtc aaccagccag gcggtcacct cgcactcccg gccggcgtag aagttgcgcg
      961 gtccccactc caggaccagc acccggtcgg cgttcaactc cttcagcgcc cggtatggtt
     1021 cgggccgcac ctggttccaa agcgttacca ggccgccgaa catcggccag cagctctcgt
     1081 catagtggta gagaatgtac ccgccgcagt aagggttgat cagcaaaccc tccagcaggc
     1141 gccgggttcc ctcggcggcg gcctggccgg tcaggcgccg gaactcgtcc accgatcgga
     1201 agatcccttc caggccggcc tgccggtagg cggtcaggaa ggcctcccac tcccggctga
     1261 agattcggta gtcgttcaac cccttccagg catgccggcg ccggtattcc tttaatatct
     1321 cgggcagtcg gccgtaatca ccgcagccga tctcggtgac aaaggtcagc cggttccggg
     1381 ccccggtcag cgaaaagttc cagcgtttat tgacctggaa gacccggccc ggctggccgg
     1441 cggtggtaag gaactcccag aattcccggt cggccggcag tccggggtaa cggtggatat
     1501 cgttgaccgg aaccggccgg tcccggccgg gcaggtagag gtagggctgg ccgaactgga
     1561 cgccgccgga gt
//
LOCUS       DAOQYN010000311         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_100384_length_1550_cov_3.679599, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000311 DAOQYN010000000
VERSION     DAOQYN010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100384_length_1550_cov_3.679599"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PKN80_09200"
     CDS             <1..1022
                     /locus_tag="PKN80_09200"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNQ36222.1"
                     /translation="KRRTEILPEAAEMNELDLIKPEDVVVTLSAAGYIKRVPLDTYRR
                     QRRGGRGMLASSSKEEDYLKYLVLSNTLDYLLFFTNLGRVHWLRAYQVPEQSRQAKGR
                     PIINLLRLQENETVAALIPVNEFKTDQFLLMVTERGLVKKTSLASYSRPRNGGIIAMG
                     LRPEDRLVGVRVTGGQYEIFLSTRRGMAIRFPETNTRAMGRTATGVIGVRLRDGDVVV
                     GGEALGEAVPLLTVTEKGYGKRSLSSLYRLQKRGGKGIIDIHVLDRKDKVVAMRVVKD
                     SDEILILTQKGQAIRIKGSEIRCIGRNTKGVKLINLLPGDRITDFVVSEIDRAEEEAR
                     EEGDA"
BASE COUNT          301 a          512 c          480 g          257 t
ORIGIN      
        1 acaagcggcg gaccgagatc ctgcccgagg ccgccgagat gaacgagctg gacctgatca
       61 agcccgagga cgtggtggtg accctttcgg ccgccggcta catcaagcgg gtcccgctgg
      121 acacctaccg ccgccagcgg cggggcggcc ggggcatgct ggccagcagt agcaaggagg
      181 aggactacct gaagtacctg gtcctctcca acaccctgga ttacctgctc ttcttcacca
      241 acctgggccg ggtgcactgg ctccgggcct accaggtccc ggaacagagc cgccaggcca
      301 aggggcggcc gatcatcaac ctcctgcgcc tgcaggagaa cgagaccgtg gccgcgctga
      361 tcccggtcaa cgaattcaag accgaccagt tcctgctcat ggtcaccgag cgcggcctgg
      421 tcaagaagac ctcgctggcc tcctacagcc ggccccggaa cggcggcatc atcgccatgg
      481 gcctgcggcc ggaggaccgg ctggtcggag tgcgggtgac cggcggccag tacgaaatct
      541 tcctctccac ccgccggggc atggccatcc gcttccctga aaccaacacc cgggccatgg
      601 gccggacggc caccggcgtg atcggcgtcc gcctgcgcga cggcgacgtg gtggtcggcg
      661 gcgaggccct gggcgaagcg gtccctctgc tcaccgtgac cgagaagggc tacggcaagc
      721 gttcgctctc cagcctctac cggctccaga agcggggcgg caagggcatc atcgacatcc
      781 acgtcctgga ccggaaggac aaggtcgtcg ccatgcgggt ggtcaaagac tcggacgaga
      841 tcctgatcct cacccagaag gggcaggcca tccggatcaa gggctcggag atccgctgca
      901 tcggccgaaa caccaaggga gtgaagctga tcaacctcct gcccggagac cggatcaccg
      961 acttcgtggt cagcgaaatc gaccgggcgg aggaggaggc ccgggaagag ggcgatgcct
     1021 gagcgggctt gaccggcccc gcgcattgag agcggggcgg cggttgcagg acggaaacaa
     1081 tggccggcct ggtcatgaaa atgaccacgg ccggccgctt ctttgccgcc cgccgcgtcc
     1141 gacgcgccag gacggcaccg ccgcgcgctt tctcctccct ttctccacag tcatacacca
     1201 gcctttattt agatggaggc ccgggttatt tgactttccg cccccgcgcg tgctactaaa
     1261 aagccggcat catcgatcac gcctttccac tcccaaccga tcggccggga ttctttccgg
     1321 taaagggccg gcgcgatgag aaatggccga atactatttt tggggttgat tctcccccgg
     1381 atttcccatc ccgcatcctt cctgccggca aggaaattaa attgaagatg aagatgaaaa
     1441 gaaccttggt tttcctgacc cttttggcca gtttctttat tattccccta attctccacg
     1501 cccaggcgca gatcaccgcc gagcagttgg agaagatccg gggccgcaag
//
LOCUS       DAOQYN010000312         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_101645_length_1538_cov_4.485502, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000312 DAOQYN010000000
VERSION     DAOQYN010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101645_length_1538_cov_4.485502"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PKN80_09205"
     CDS             <1..834
                     /locus_tag="PKN80_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNQ36223.1"
                     /translation="MKFLRNHNVPVTSDPYEAVSCLGAMYAYYRSLSDGRGTPEPVAI
                     DRAGINRLAEAARADGRSFLLADEAQRLMELAGITTAGSRVATSAAAAVKAAGEIGYP
                     VVLKVVSRDILHKSEAGGVLLDLGTAAAVRAGYRRIMDSCRAYNPEARIKGVEVCEQV
                     KPGTELILGARRDASFGPIVMCGMGGIYVEVLKDVTFRSFPLTRKEADRMLKDLRVYP
                     ILSGVRGQKSRDTATVVEALLKLGSVVKECPLISDIEINPVVVYQKGLKAVDVRVLLS
                     K"
     gene            838..1428
                     /gene="hisB"
                     /locus_tag="PKN80_09210"
     CDS             838..1428
                     /gene="hisB"
                     /locus_tag="PKN80_09210"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNQ36224.1"
                     /translation="MGRKAKVYRKTRETEIEVTLDLDGTGKAAVELELGFLKHMITAF
                     AQHGLLDLSVRASGDLEVDEHHLTEDLGLVIGEALNQALGDKKGLRRFGFASVPMDES
                     LVETSLDFSGRPFLALELPKLPRRKGYFEFADAREFLRALAQAGRITLHVECRRGDNQ
                     HHILEALFKSLGRAVRQAVELEPRLKGRIPSTKGTL"
BASE COUNT          290 a          476 c          512 g          260 t
ORIGIN      
        1 atgaagttcc tgcgcaacca caacgtgccg gtgaccagcg atccttacga ggcggtctcc
       61 tgcctggggg ccatgtacgc ctactaccgg tccctatccg acggccgggg aaccccggaa
      121 ccggtggcga tcgatcgcgc gggcatcaac cggctggccg aggcggcccg tgccgacggc
      181 cgctctttcc tgctggccga cgaggcgcag cgtttgatgg aactggccgg gataacgacc
      241 gccggcagcc gggtggccac cagcgcggcc gccgcagtca aggcggccgg ggagatcggt
      301 tacccggtgg tgctcaaggt ggtttcccgg gacatcctgc acaagagcga ggccgggggt
      361 gtcctgctcg acctgggaac ggccgcggcg gttcgggccg gataccggcg catcatggac
      421 agttgccggg cctacaaccc ggaagcgaga atcaagggag tggaagtttg cgagcaggtc
      481 aagcccggca ccgagttgat cctgggcgcc cgccgcgatg cttcgttcgg cccgatcgtt
      541 atgtgcggaa tgggcggcat ttacgttgag gtccttaagg acgtcacctt ccgttctttc
      601 ccgctgacgc gtaaagaggc ggaccggatg ctcaaagacc tgcgcgtcta tccgatcctg
      661 tccggagtcc gcggccagaa atcgcgcgac accgccacgg tggtcgaagc gctgttgaag
      721 ctgggctcgg tggtgaagga gtgcccgctg atttccgaca tcgagataaa cccggtagtc
      781 gtttaccaaa aggggttgaa agcggttgac gttcgcgtcc tgctttcaaa atgagcgatg
      841 ggcagaaaag ccaaggttta ccgcaagacc cgcgagaccg agatcgaggt gacgctggac
      901 cttgacggca ccggtaaggc cgcggtggag ctggaactgg gttttttgaa gcacatgatt
      961 accgccttcg cccagcacgg actgctggat ctttcggtcc gggcttccgg agacctggag
     1021 gttgacgaac accatctgac cgaagatctc ggtctggtga tcggggaggc gctcaaccag
     1081 gcgctgggcg acaagaaggg gctgcgccgt ttcggattcg cctcggtgcc gatggacgag
     1141 tccctggtcg aaaccagcct cgacttttcg ggacgcccct tcctggccct ggagctgccc
     1201 aaactgcccc ggcgcaaggg gtacttcgaa ttcgccgatg cccgggagtt cctccgggcc
     1261 ctggcccagg ccggtcggat caccctccac gtggaatgcc ggcggggcga caaccagcac
     1321 catatcctgg aggccctctt caagagcctg ggccgggcgg tgcggcaggc ggtcgagctg
     1381 gaaccgcgcc tgaaaggcag gataccttcc accaaaggca ccctttaaaa atggccgaag
     1441 aaaagaagcc ggacgccccg gttgagtcac ccatcaccgg gccggacgag caccacgccg
     1501 gggaactcct gcagcagttg aaggccaccc tctccgcc
//
LOCUS       DAOQYN010000313         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_106705_length_1494_cov_5.596942, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000313 DAOQYN010000000
VERSION     DAOQYN010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106705_length_1494_cov_5.596942"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKN80_09215"
     CDS             complement(<1..523)
                     /locus_tag="PKN80_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36225.1"
                     /translation="MSRRILPVLLMMLLSAGPAFSQQGRFSFTRLWTGEELQQLNLGG
                     GKFPGNPKEWQSSAGVAFGFNYPGTGPLPEPIVVKIPLGRTLPLGHYRLYVKNFYLGK
                     MEATLGDITKPLRIVRFDWSVVETFEINEPADQVTLRYFPTDLVAGSGKAHQQYYILQ
                     GLFITTEANKVPKF"
     gene            complement(749..1420)
                     /locus_tag="PKN80_09220"
     CDS             complement(749..1420)
                     /locus_tag="PKN80_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ36226.1"
                     /translation="MKSRKTARGFTLIELLVVIGIIAILAAMLLPALSRARNRAKMAA
                     CVNNLKQIGLASRMYMNDNDGTIPALYSDGPTFYGATGSNFGNIVGIGSIYYGHWMGQ
                     LAPYLKCDLTSHITISRNRIFSCPSRSGKKFYYSCNIGGIGGTGVRITQIRKDITRLI
                     HITELYHVYNHAITGSDKYCDYDRHDGQINALMLDGHVENGDINKFGTAYTSTGVGKE
                     YWTGI"
BASE COUNT          279 a          400 c          470 g          345 t
ORIGIN      
        1 cgaacttggg caccttgttg gcctcggtgg tgatgaaaag gccctgcagg atgtaatact
       61 gctggtgggc cttgccgctg ccggccacca ggtcggtcgg gaagtagcgc agggtgacct
      121 ggtcggccgg ctcgttgatc tcgaaggtct cgaccacgct ccagtcgaag cggacgatgc
      181 gcagcggctt ggtgatgtcg cccagggtcg cctccatctt gcccaggtag aagttcttga
      241 cgtagaggcg gtaatggccc agcggcaggg tccggccgag cggaatcttc accacgatcg
      301 gctccggcag cggaccggtg cccgggtaat tgaaaccgaa ggccacgccg gccgaagatt
      361 gccattcctt cggattgccg gggaacttgc cgccgcccag gttgagttgc tgaagctcct
      421 cgccggtcca gagtcgggtg aaactgaaac gtccctgctg actgaaggcc ggcccggccg
      481 agaggagcat catcaacagg accggaagta ttcttcttga catgaccgcc tccgtttcga
      541 tgggccttcc ttagcaaaaa tccggttggt tagacaaaaa gaaaagggac ttcgttcatt
      601 ataacattgt ttctttgttt ttttaacccg gcggtgaaac ggtctttaat ttattggcgt
      661 ttcggtcggg tggcgccctt tgaattcatt aaaaaagagg gcgtgccgac ccggcgcgcc
      721 ccctttttaa accttatatg ccgaatcgtc agatgccggt ccagtactcc ttgcccaccc
      781 cggtactggt gtaggcggtt ccgaacttgt tgatgtcccc gttttcgacg tgaccgtcca
      841 gcatcagggc gttgatctgt ccgtcgtggc ggtcgtagtc acagtacttg tcactaccgg
      901 ttatggcgtg gttgtaaaca tggtaaagtt cggtgatgtg aatgagccgg gtgatgtcct
      961 tgcggatctg ggtgatgcgg actccggtgc cgccgatgcc tccgatattg caggaataat
     1021 aaaacttctt tccggagcgg gacgggcagc tgaagatgcg attccgggag atggtgatgt
     1081 ggctggtcag gtcgcacttc aggtagggcg ccagctggcc catccagtgg ccgtaatata
     1141 tgcttccgat cccgacaatg ttgccgaagt tggagccggt cgcaccgtag aaggtcgggc
     1201 cgtcgctgta tagggccgga atggtcccgt cattatcgtt catgtacatc cgggaggcca
     1261 ggccgatctg cttcaggtta ttcacgcagg cggccatctt ggcccggttg cgcgcccggg
     1321 acaaggccgg cagcagcatg gccgccagga tggcgatgat gccgatcacc accaggagtt
     1381 caatcagggt gaagccccgg gccgttttcc ttgatttcat aagtcctccg tcactatgtg
     1441 attggttggg cggatggtta cggggttacc gtcagccggg ccgtgccgac gttg
//
LOCUS       DAOQYN010000314         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_107772_length_1485_cov_3.689510, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000314 DAOQYN010000000
VERSION     DAOQYN010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107772_length_1485_cov_3.689510"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..>1485
                     /locus_tag="PKN80_09225"
     CDS             109..>1485
                     /locus_tag="PKN80_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNQ36227.1"
                     /translation="MSIASIPTRRAIHLDFHTMPAVYDVGRDFDPEAFAAALADSGVD
                     YITVFAKCNLGFTYYPSRIGLPHPGLIPSKADLLGPMVEAGHRRGIKVAAYFNAGIDH
                     EHALRNRQWSKVRADGRVYAIEQKGHSFRGLCLNTGYRDYLLGMIGEVLEAYPVDGIF
                     LDCFNLSPCYGVECLEGMEKLGMDKFDDRQARDYCRLVTERFMADAARLVRKLSRRPT
                     QLYFNGMPYRQQPTHLELEVLPTGGWGYEHLPFAIRYARTLGKPYFTMTGRFHLGWGD
                     FGGLRTFHSLLYDCYYSISNGGTCSIGDHMHPRGRLEPAVYDLVKRVYRQVRELEPWT
                     DRASSLAEAAILHPGLEHFPSPAPGFGPEELASVKGATRLLRELQCQFDLVDGRAGLE
                     KYRLLLLPDSVRLDPGLAAKLRAHLKRGGLLVSSAFAGLNPAGTGFALKEYRLKYLGP
                     EAHHYTFFK"
BASE COUNT          237 a          521 c          444 g          283 t
ORIGIN      
        1 atttgcgcgg aggccttttt agatgcttaa ataaggaaag cctttgccgc cgcgtgcatt
       61 ttgttaccga tatcactgtt taccatttta ccattttcgg gagacgccat gtccatcgct
      121 tcgattccca cccggcgggc catccacctt gatttccaca ccatgccggc cgtttacgat
      181 gtcggccgcg atttcgaccc cgaggccttc gccgccgcct tggccgattc cggggtcgat
      241 tacatcaccg tcttcgccaa gtgcaacctg ggcttcacct actacccgag ccggatcggc
      301 cttccccatc ccggcctgat tccgtcgaag gccgacctgc tggggccgat ggtcgaggcc
      361 ggccaccggc gtggcatcaa ggtggccgcc tatttcaacg ccggcatcga ccacgagcac
      421 gctctgcgca accggcaatg gtcaaaggtg cgggccgacg gccgcgtcta cgccatcgag
      481 cagaaggggc acagcttccg cggcctctgc ctgaacaccg gctaccggga ttacctgctg
      541 ggaatgatcg gcgaggtgct ggaggcctac ccggtcgacg gcatcttcct cgactgtttc
      601 aacctcagcc cctgctacgg ggtcgagtgc ctggagggga tggagaaact ggggatggac
      661 aagttcgacg accgccaggc gcgcgattac tgccggctgg tcaccgaacg tttcatggcc
      721 gacgccgccc ggctggtgcg caaactctcc cggcggccga cccagctcta cttcaacggc
      781 atgccctacc ggcagcagcc gacccacctg gaactggagg tgctgcccac cggcggctgg
      841 ggctacgagc acctgccctt tgccatccgt tacgcccgga cactcggcaa accctacttt
      901 accatgaccg gccgttttca cctcggctgg ggcgatttcg gcgggctgcg caccttccac
      961 tcgctccttt acgactgcta ctactcgatc agcaacggcg gtacctgctc gatcggcgac
     1021 catatgcatc cgcgcggccg gctcgaaccg gccgtttacg acctggtcaa gcgggtttac
     1081 cggcaggtcc gggaactcga gccctggacc gaccgggctt cgtcccttgc cgaggcggcc
     1141 attctccatc ccggcctgga gcatttcccg agcccggcgc ccgggttcgg ccccgaggag
     1201 ttggcctcgg tcaagggcgc gacccggctg ctgcgggaac tccagtgcca gtttgacctg
     1261 gtggacggcc gggccggcct ggagaagtac cggctcctgc tcctgccgga ctcggtccgg
     1321 ctcgatcccg gcctggccgc gaagctgcgc gcccacctga agcggggagg cctcctggtc
     1381 agttccgcct tcgccggtct caacccggcc gggaccggtt tcgccctcaa ggagtaccgg
     1441 ctcaagtacc tggggccgga ggcgcatcac tacaccttct tcaag
//
LOCUS       DAOQYN010000315         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_108783_length_1477_cov_2.822082, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000315 DAOQYN010000000
VERSION     DAOQYN010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108783_length_1477_cov_2.822082"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PKN80_09230"
     CDS             <1..340
                     /locus_tag="PKN80_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ36228.1"
                     /translation="FVAAGVMLPIMTIMADALMIFGGYLVGTTTMNLSSGLYLYQAFR
                     FVLFRDIMIGILKAFLFGMIIVIVSSREGFHAEGGAKGVGRATTTAVVVSLFITILAD
                     LLVTAIFYFI"
     gene            390..1193
                     /locus_tag="PKN80_09235"
     CDS             390..1193
                     /locus_tag="PKN80_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNQ36229.1"
                     /translation="MKQEKKHNQVTFGVLGMHCASCVRTVEKSLRKIRGVGQVSVNLT
                     EEKATVDYDPDRVTPEKIFRVTRAAGYEPVELQPEKPGEYRKTRAERADGLRRRFIFA
                     ISFAVPLMAVAMAGMLWPDLPMRTHQFLVLLQFLLATPVIGAGTIFYRPGVLGLFRNR
                     AANMDTLITLGVGTAYLYSLFQSIRVWSGPLTAPPHLYYEVAAFLLAFILLGKFLEAR
                     ARGRTSEAIQKLIALQPPTAVRIQSGREREVPVAAITPGDILVVRPDPG"
     gene            1171..>1477
                     /locus_tag="PKN80_09240"
     CDS             1171..>1477
                     /locus_tag="PKN80_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36230.1"
                     /translation="MSGRIPVDGVVTEGDSSVNESMVTGESIPVEKTAGSQVIGGTIN
                     LSGAFRFRAVRVGRETFLAQVIRLDDEDRRITLSLKSAEGSEVRSEMKYDPEAQKARP
                     "
BASE COUNT          274 a          487 c          441 g          275 t
ORIGIN      
        1 attcgtggcg gccggcgtaa tgctgcccat catgaccatc atggcggacg cgctcatgat
       61 cttcggcggc tacctggtcg gcaccacgac catgaatctg agcagcgggc tttacctcta
      121 ccaggccttc cggttcgtgc tcttccggga catcatgatc gggattctca aggcttttct
      181 cttcggcatg atcatcgtga ttgtctcaag tcgcgagggt ttccacgccg aaggcggcgc
      241 caagggagtc ggacgggcga ccaccaccgc agtagtggtt tcgcttttca tcaccatcct
      301 ggccgaccta ctggtcaccg ccatcttcta cttcatttga ggccggtccg agcccggaac
      361 ccggcgcgga cggttccgca caacccggca tgaagcaaga gaaaaaacac aaccaggtta
      421 ctttcggcgt gctgggaatg cactgcgcca gttgcgttcg aaccgtcgaa aaatcgttgc
      481 ggaaaatccg cggcgtcggc caggtcagcg tcaacctgac cgaggaaaaa gcgacggtcg
      541 attatgaccc ggaccgggta accccggaaa aaatctttcg ggtgacccgc gcggccggat
      601 acgaaccggt ggagttgcaa ccggaaaaac ctggcgaata ccggaagacc cgggccgaac
      661 gggctgacgg gcttcgccgc cgcttcattt ttgccatttc cttcgcggtt ccgctgatgg
      721 ccgtggccat ggccggtatg ctctggccgg acctgccgat gcgcacccac cagttcctgg
      781 ttcttctcca attcctgctc gccaccccgg tcatcggggc cggaacgatc ttctaccgac
      841 cgggagtgct gggcctcttc aggaaccggg cggccaacat ggacacgctg atcactctcg
      901 gcgtgggcac cgcctatctc tacagcctct tccaatcaat ccgggtctgg agcggcccgt
      961 tgacggcacc cccccacctt tactacgagg tggccgcctt tctgctcgcc ttcatcctgc
     1021 tcgggaaatt cctcgaggcc cgggcccggg gccggacctc tgaggccatt caaaaactga
     1081 tcgccctgca gccgccgacc gccgttcgga tccaatccgg ccgggagcgg gaagtcccgg
     1141 tggcggccat cactcccggc gacattctgg ttgtcaggcc ggatcccggt tgacggcgtg
     1201 gtaaccgaag gcgactcctc ggtaaacgaa tcaatggtca ccggtgaaag catcccggtc
     1261 gagaagacag ccggcagcca ggtcattggc ggcaccatca atctgtcggg agccttccgc
     1321 ttccgggccg tgcgggtggg ccgggagacc ttcctggccc aggtcatccg cctggacgac
     1381 gaggaccgcc gcatcacgct gtcgctcaag agcgccgagg gctccgaggt gcgcagcgag
     1441 atgaagtacg accccgaggc ccagaaggcg cgccccc
//
LOCUS       DAOQYN010000316         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_109842_length_1468_cov_3.433121, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000316 DAOQYN010000000
VERSION     DAOQYN010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109842_length_1468_cov_3.433121"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1468)
                     /locus_tag="PKN80_09245"
     CDS             complement(<1..>1468)
                     /locus_tag="PKN80_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36231.1"
                     /translation="RKSEIEIMKATGQNADRTMPDDIFRMVRLGPDAIETLSVADEEG
                     WLALIHVEGLSKGYPDDWNYCRTWMEMYPKLVANHPSAVIWQIWSGGYQPGPHGHPMS
                     IGALVPEEIVKNEWHYRRNRFLKEFDPERRPFYYYTGGTGAEIRAIMMSLGYGTPIQE
                     MEEWLSYWAAHQTDPFLPVEQQIFSRPDYFWQRGSKESIVLEYYAKFFGEAAYRKATA
                     ELVNRWQDNKGNQWATPEYWTDFYNLAYTRAIRSWRTYGLSGCLFHVSFFFPALYEKG
                     RLGGRLTSFGETISRVNAPVLFYLAGPPEDFVGKDHNYFGGEKARKSGVLINQTFHPL
                     AGRMSWRLETADGRELTAGSAPVTVEPGRRLFQPMEFELPAVREKTRLRITARFEETG
                     SDTVRSDSFRLTLFPKREAAAPGKPVALIDETGETAEALKNLGVSCQLFNAETITAGR
                     TSFQDYQLLVIGKNSYPAAVKIFTENIPFVRQVGEGLNV"
BASE COUNT          223 a          473 c          488 g          284 t
ORIGIN      
        1 gacgttcagc ccttcgccga cctggcgcac gaagggtatg ttctcggtga aaattttcac
       61 cgcggccggg tagctgtttt ttccgatgac cagcagttga taatcctgga agctggtccg
      121 gccggcggtg attgtctcgg cgttgaataa ctggcaagac acgcccagat ttttcagggc
      181 ctcggcggtt tcgcccgttt cgtcgatcag ggccaccggc ttgcccgggg ccgccgcctc
      241 ccgcttcggg aagagggtca gccggaaact gtcggaccgg accgtgtcgc tgccggtctc
      301 ctcgaaacgg gccgtgatcc gcagccgtgt cttctcccgg accgccggca gctcgaactc
      361 catcggctga aaaaggcggc ggcccggctc gaccgttacc ggggccgaac cggccgtcag
      421 ctcccggccg tcggccgtct ccagccgcca gctcatccgg ccggccagcg ggtggaaggt
      481 ctggttgatg agcacgccgc tcttgcgcgc cttctcgccg ccgaagtagt tgtggtcctt
      541 gcccacgaaa tcctccggcg gcccggccag gtagaagagc accggcgcgt ttacccggct
      601 gatagtctcc ccgaaggagg tgagccggcc gcccaggcgc cccttttcgt agagggccgg
      661 gaagaagaag gagacgtgga agaggcagcc gctgagtccg taggtgcgcc aggagcggat
      721 cgcccgggtg taggccaggt tgtagaagtc ggtccagtac tccggggtcg cccactggtt
      781 gcccttgttg tcctgccagc ggttgaccag ctcggcggtc gccttgcggt aggccgcctc
      841 gccgaagaac ttggcgtagt attcgaggac gatcgattcc ttgctgccgc gctgccagaa
      901 gtagtcgggc cggctgaaga tctgctgctc caccggcagg aagggatcgg tctggtgggc
      961 cgcccagtaa gacagccact cctccatctc ctggatgggc gtgccgtagc ccagcgacat
     1021 catgatcgcc cggatctcgg cgccggtgcc gccggtgtag tagtagaagg gacgtctttc
     1081 cgggtcgaac tccttcagaa accggttccg gcggtagtgc cactcgttct tgacgatttc
     1141 ctccggcacc agggcgccga tgctcatcgg gtggccgtgc gggccgggct ggtagccgcc
     1201 gctccagatc tgccagatga ccgccgaggg gtgattggcg accagcttcg ggtacatctc
     1261 catccaggtc cggcagtagt tccagtcgtc cgggtatccc ttggaaaggc cctcgacgtg
     1321 gatcagggcc agccagccct cctcgtcggc cacgctcagg gtctcgatgg cgtccggccc
     1381 cagccgcacc atccggaaga tgtcgtcggg catggtccgg tcggcgttct ggccggtggc
     1441 cttcatgatc tcgatttcgc tcttgcgg
//
LOCUS       DAOQYN010000317         1462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_110605_length_1462_cov_3.643923, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000317 DAOQYN010000000
VERSION     DAOQYN010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110605_length_1462_cov_3.643923"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PKN80_09250"
     CDS             complement(<1..1104)
                     /locus_tag="PKN80_09250"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ36232.1"
                     /translation="MPGIEVFDQREEEMVLDIIRRRMIYRYTYNDTNYQQSYCYRFET
                     AFNQYLGSRYSLAVSSGTAALRCALAALGVKPGDEVIVPAYTFIATLEAVLDAGAKPV
                     LAEIDDTLNLDPAGLEPLVTPRTVGIIPVHMLGAAAEMKEILAFARGRKLFVLEDACQ
                     AVGARYGGQAVGTLAEMGTFSFDSVKLLTCCEGGLVVTKDPEFYRRADRYHDHGHIHN
                     LSLPRGREPKEGAGFNYRISEIHAAIGLAQLQKLPDMLENLHRNKNRIKNGLKLPEGF
                     SFRRLADPHDNATFLAVLAPDGTRAEAVRKEMAKGGVAPAVLNYWHFEVNRELAGGSY
                     PKTGELLDRAIVLPINIRMEPVQLEKSGAVFQAA"
     gene            complement(1123..>1462)
                     /locus_tag="PKN80_09255"
     CDS             complement(1123..>1462)
                     /locus_tag="PKN80_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNQ36233.1"
                     /translation="IDRRFKAAVSDSGGDITASYYCPHRPDEGCACRKPGTGLIDRAA
                     RELNLDLKRSRAYFVGDRQTDIATGRRAGLVTILVLSGHTRLAGDTRDWPVPPDHTAP
                     DLLGALNYIL"
BASE COUNT          206 a          451 c          534 g          271 t
ORIGIN      
        1 ggcggcctgg aagacggcgc cgctcttctc cagctggacc ggctccatcc ggatgttgat
       61 gggcaggacg atggcccggt cgaggagttc cccggtcttc gggtagctcc cgccggccag
      121 ttcccggttg acctcgaagt gccagtagtt caggacggcc ggggcgacgc cacctttggc
      181 catctccttc cggaccgcct cggcgcgcgt tccgtcgggg gccagcaccg ccaggaaggt
      241 ggcattgtcg tgcgggtcgg ccagccgccg gaagctgaaa ccctccggca gtttcaatcc
      301 gttcttgatc cggttcttgt tgcggtgcag gttctccagc atgtccggca gcttctggag
      361 ctgggccagg ccgatcgcgg cgtggatttc cgaaatccgg taattgaagc cggcgccctc
      421 tttcggctcc cggccgcgcg gcaggctgag gttgtggatg tggccgtgat cgtggtagcg
      481 gtcggcccgg cggtagaatt ccgggtcttt ggtcaccacc aggccccctt cgcagcaggt
      541 gagcagtttc accgagtcga agctgaaggt gcccatttcg gccagggtgc cgaccgcctg
      601 gccgccgtag cgggcgccga ccgcctggca ggcgtcttcg agtacgaaga gtttgcggcc
      661 gcgggcgaag gccaggatct ccttcatctc ggccgccgcg cccagcatgt ggaccgggat
      721 gatcccgacc gtgcgcgggg tcaccagcgg ttccaggccg gccgggtcga ggttcagggt
      781 gtcgtcgatc tcggccagca ccggcttggc gccggcgtcc agcaccgcct ccagggtggc
      841 gatgaaggtg taggccggca cgatgacctc gtcgcccggc ttgacgccca gggcggccag
      901 ggcgcagcgc agggcggccg tcccggagga gacggccagg ctgtaccggg agcccaggta
      961 ctggttgaag gcggtttcga accggtagca gtaggactgc tggtagttgg tgtcgttgta
     1021 ggtgtaccgg tagatcatgc ggcgccggat gatatccagc accatctcct cttcacgctg
     1081 gtcaaagacc tcgattccgg gcatggtttt tttcggttgg cgttaaagga tatagttgag
     1141 cgccccgagc aggtcggggg cggtgtgatc gggtgggacc ggccagtcgc gggtatcgcc
     1201 ggccagccgg gtgtgtccgc tcaggaccag gatggtgacc aggccggccc ggcggccggt
     1261 ggcgatgtcg gtttgccggt cgcccacaaa gtaggcccgg ctccgtttca ggtcgaggtt
     1321 cagttcgcgc gcggcccggt cgatcaggcc ggtgccgggc ttgcggcagg cacagccctc
     1381 gtccggccgg tgcgggcagt aataggaggc ggtgatgtca ccgccggaat cggatacggc
     1441 cgccttgaag cgccggtcga tc
//
LOCUS       DAOQYN010000318         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_112940_length_1444_cov_3.684665, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000318 DAOQYN010000000
VERSION     DAOQYN010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112940_length_1444_cov_3.684665"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="tyrS"
                     /locus_tag="PKN80_09260"
     CDS             <1..963
                     /gene="tyrS"
                     /locus_tag="PKN80_09260"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNQ36234.1"
                     /translation="DFTGRIGDPTGRSETRTLLDAGTIERNLRSYREQAGRILDLDRI
                     EFVHNADWLGKLTLSDVIKTATSFTVAQMLEREDFSRRYAGGNPISLQEFLYPLLQGY
                     DSVALESDIEIGATEQKFNLLAGRTIQEIYGQEKQVVITMPILEGTDGVRKMSKSYGN
                     YIPVNAPANEMFGKLMSIPDAVMERYFRCLTDRSEAEIGALLAGHPKEAKERLARDVV
                     AFYHGEPAAAAAAAEFSRVFSQGAAPDDILTVRRPAGQATPLLELLVEAGLAPSRSEA
                     RRLIQQGGVRVNDERVADPGQTISGPGEFLIKAGKRRFLKLIKE"
     gene            976..>1444
                     /locus_tag="PKN80_09265"
     CDS             976..>1444
                     /locus_tag="PKN80_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNQ36235.1"
                     /translation="MKPILKELTRYGKLLVEKGLTTGPGGNISARDGNTIHLSPSGYS
                     LAEIGPDDWVSLDLKSGRRVGRDNGLRPTCEVSMHLACYLSRPEIRTVVHTHPVTATA
                     VATAGVKFKALFPDFVALLGREVPAVDFVTPGGEEIRAAVAGKVKQGHNVVLLK"
BASE COUNT          265 a          496 c          482 g          201 t
ORIGIN      
        1 gacttcaccg gccgcatcgg cgacccgacc ggccggagcg aaacccggac gctgctggac
       61 gccggaacca tcgagcggaa cctccgctcc taccgggagc aggccggccg catcctggac
      121 ctggaccgga tcgagttcgt ccacaacgcc gactggctcg gcaagctgac cctctcggac
      181 gtcatcaaga ccgcgacctc cttcacggtg gcccagatgc tggaacggga ggatttcagc
      241 cgccgttacg cgggcggcaa cccgatctcg ctccaggagt tcctctaccc gctgctccag
      301 ggctacgact cggtcgccct ggagagcgac atcgagatcg gcgccaccga gcagaaattc
      361 aacctgctgg ccggccggac gatccaggag atctacggcc aggagaagca ggtggtcatc
      421 accatgccca tcctggaggg gaccgacggg gtgcgcaaga tgagcaagag ttacggcaac
      481 tacatcccgg tcaacgcccc ggccaacgag atgttcggca agttgatgtc gatacccgac
      541 gcggtcatgg aacgctactt ccgctgcctg accgaccgga gcgaggccga gatcggcgcg
      601 ctcctggccg gccacccgaa ggaagccaag gaacgcctgg cccgggacgt ggtcgccttt
      661 taccacggcg agccggccgc ggcggcggcc gccgccgagt tcagccgcgt cttcagccag
      721 ggggccgcgc ccgatgacat cctgaccgtc cgacgtccgg ccggccaggc gacgccgctg
      781 ctcgaactgc tggtcgaggc cggcctggcg cccagccgca gcgaggcccg ccggctcatc
      841 caacagggcg gggtccgggt caacgacgag cgggtggcgg atcccgggca gaccatttcc
      901 ggcccgggcg aatttctgat caaggccggc aaacgccgtt ttctgaaatt gatcaaggag
      961 taggggaggg gcctggtgaa accgattctc aaggagctga cccgctacgg aaaactgctg
     1021 gtggagaagg ggctgacgac cggcccgggc ggcaacatca gcgcccgcga cggaaacacg
     1081 attcatcttt caccgagcgg ctacagcctg gcggagatcg ggccggacga ctgggtgagc
     1141 ctcgacctga agagcggccg gcgcgttggc cgggataacg gcctgcggcc cacctgcgag
     1201 gtctcgatgc acctggcctg ctacctcagc cgccccgaga tccggaccgt cgtccatacc
     1261 cacccggtga ccgccaccgc ggtggccacc gccggcgtga agttcaaggc cctcttcccc
     1321 gatttcgtcg ccctgctcgg ccgcgaagtc ccggccgtcg atttcgtcac gcccggcggc
     1381 gaggagatcc gggcggcggt cgccggaaag gtcaagcagg ggcacaacgt ggtgctgctg
     1441 aaga
//
LOCUS       DAOQYN010000319         1440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_113477_length_1440_cov_3.992058, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000319 DAOQYN010000000
VERSION     DAOQYN010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113477_length_1440_cov_3.992058"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..735
                     /locus_tag="PKN80_09270"
     CDS             13..735
                     /locus_tag="PKN80_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36236.1"
                     /translation="MKLRGRVLVLAGLGLLLTAGLLLAENPAGADGGKAPGTEKPRPG
                     IEKRVQGGDGGQWGALAQLGLSEEQKASIKSILDTQRERMNSVRQDSTLSREETKKKL
                     AEIEKTGQEQVAALLTTEQKAKLAAWKEKQRADQERKMAERLADRLQLDTEQRVKILP
                     LLKERQAKVQAVQNDQTISNEARKEKLREIHQTYQGKIVQYLSEEQKAKFTERPNGDR
                     PKPLAQPPLPPAPPQPDAKPTA"
     gene            complement(991..>1440)
                     /locus_tag="PKN80_09275"
     CDS             complement(991..>1440)
                     /locus_tag="PKN80_09275"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36237.1"
                     /translation="INYERLVEALTRGKVEVQYLLTPLIIPVTISEMLVWEEGQPRFQ
                     ALGFSTSRWEKGQVAIHSHPALINRPETALRNLLAEKDTPAADPETLARRACHNSIRS
                     GEPAGREEAEALRDRLLACADPFTCPHGRPTVLELTERFLDRQFQRA"
BASE COUNT          319 a          412 c          500 g          209 t
ORIGIN      
        1 aaaggagagg gcatgaaatt gagaggcaga gtgctggtgc tggcgggatt gggattgctg
       61 ttgacggccg ggctgctcct ggccgagaat ccggccggtg ccgacggcgg caaggcaccc
      121 gggacggaga agcctcggcc ggggattgaa aaacgcgtcc agggcggaga cggcggacag
      181 tggggagccc tggcccagct gggcctgagc gaggagcaga aggcctcgat caaatccatc
      241 ctggatactc agcgggaacg gatgaacagc gtgcgccagg acagcacgct cagccgggag
      301 gagacgaaga agaagctggc cgaaatcgag aagaccggcc aggagcaggt ggccgctctt
      361 ctaaccacgg agcagaaggc gaagctggcc gcctggaagg agaagcaacg ggccgatcag
      421 gaacggaaaa tggctgaacg gctggccgac cggttgcaac tggacaccga gcagcgggtt
      481 aagatactgc cgcttttgaa ggaaaggcag gcaaaggtcc aggcggtgca gaacgaccag
      541 accatttcca acgaggctcg caaggagaaa ctccgggaga ttcatcagac ctaccagggc
      601 aaaattgttc aatacctgag cgaggagcag aaggcgaagt tcacggaacg gccgaatggg
      661 gaccggccca agccgctggc ccagccacca ttgccgccgg cgccgccgca gccggatgcc
      721 aaaccaaccg cctgattcca cccggattga attaaaaagg cggcctcgcc ggaaggcggg
      781 ccgccttttt ttgttttcgg ccgcacggcc aacaaagctg gccggtccga aggcagacca
      841 ctatcaggcg ctggtgatcc tgatcaatag tggtttgaat tcgggccggg agcgcgacgg
      901 ccaggagaag cccaggaggc gtactgaagg tacgccgacg ccggcgccga cgcagccaac
      961 aaagctatcc ggtccgaagg cagaccacta tcaggcgcgc tggaactgcc ggtcaaggaa
     1021 ccgctccgtc aattccagca cggtcggccg gccgtgcgga caggtgaagg gatcggcgca
     1081 ggccaggaga cgatcccgga gcgcctcggc ttcctcgcgt ccggctggtt cgccggaccg
     1141 gatggaatta tggcaggcgc gccgggccag ggtctccggg tcggcggccg gagtgtcctt
     1201 ttcggccagc aggttgcgca gggccgtctc cgggcggttg atcagggccg ggtgggagtg
     1261 gatggcaacc tgtcccttct cccatcggct ggtggagaaa ccgagtgctt ggaaacgcgg
     1321 ctgtccttcc tcccagacca gcatctccga tatggtaacc ggtattatca ggggcgtcag
     1381 caggtactgg acctcgacct tgccccgggt caaagcctcg accaggcgct cgtaattaat
//
LOCUS       DAOQYN010000320         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_113989_length_1436_cov_3.781318, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000320 DAOQYN010000000
VERSION     DAOQYN010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113989_length_1436_cov_3.781318"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKN80_09280"
     CDS             <1..352
                     /locus_tag="PKN80_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36238.1"
                     /translation="REAGLREMVRVLKPGGRLYLLILPPGYYLRRFNLVLRSPIWLGY
                     FLLVFFNGCLFSIGGFQITLRLGKRRLSELYFTQTSLARKLEQLGLEIIESWRNPETG
                     LASTLELIAVKRPG"
     gene            complement(451..>1436)
                     /locus_tag="PKN80_09285"
     CDS             complement(451..>1436)
                     /locus_tag="PKN80_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36239.1"
                     /translation="WPGGVETTYKTVPDGLKDYVAYYALMVGQLGGREAATLLVQRLK
                     EDYPLVQPAAGAVSSIVRPGMDPYAPGPGGVPSEGPSGNEPAPAVVSEAVLKEEIRFR
                     VEAVKVLSIIAGENLLPFLQYLAKNDIKPEVRTMASSILGTVETRIAASRDDLRQAAS
                     ALAANRFDEAIRLYKQAYEKNPSPRVTREYEKSKLIIGAQYLAGNRKAEAEPLLKPLF
                     DALEVKVYQKLPEEMVKAKMTPEKQQVMAQVEAKKQAVIDDIKAKLSANGLTQAEIQR
                     ADPNRFFNYGIEKGWLVEEATLMEMPPGEPGGPMPGQPGYGQGGMMPAPAP"
BASE COUNT          260 a          457 c          410 g          309 t
ORIGIN      
        1 tcgcgaagcg gggctgcgcg agatggttcg ggtactgaaa cccggcggcc ggctctacct
       61 gctcatcctg ccgcccggct actacctgag gcgcttcaac ctggtactcc gttccccgat
      121 ctggcttggt tatttcctgc tggtcttctt caatggctgc ctcttctcga tcggcgggtt
      181 tcaaatcacc cttcgactgg gtaagcgtcg cctttccgaa ctctacttca cccaaacctc
      241 gctggcccgc aaactggagc agctcggatt ggaaatcatt gaatcctggc gtaatcccga
      301 aaccggtctg gcttcgacgc tggaattgat tgcagtcaag cggcctgggt gatctcgaag
      361 gcagggtagg ggggatgcgg taaaggacgg cggttaaaaa aactcccggc ggcaagggcc
      421 gccgggagtt gtgcttttta aaacaggact tcagggggcc ggagccggca tcattccgcc
      481 ctgaccataa ccgggctgcc cgggcatcgg acccccgggc tccccgggcg gcatctccat
      541 aagcgtcgcc tcttcaacca gccagccctt ctcaataccg taattgaaga agcggttcgg
      601 atcggcccgc tggatctcgg cctgggttaa accgttggcg gaaagcttgg ccttaatatc
      661 gtcaattacc gcctgtttct tggcctcaac ctgcgccatg acctgctgct tttcgggggt
      721 catctttgct ttcaccattt cttccggcag tttctggtaa accttgactt ccagagcatc
      781 aaacaagggt ttcaaaagcg gttcggcctc agccttccgg ttgccggcca ggtactgggc
      841 cccgataatt aacttgctct tctcatactc gcgggtaacc cgcggtgaag ggttcttctc
      901 gtaggcctgc ttgtaaagac gtatcgcctc gtcaaaccgg ttggccgcca gggcgctggc
      961 cgcctggcgc agatcgtcac gggaagcggc gatcctggtc tcaaccgtgc ccagtatgct
     1021 ggaggccatg gtccggactt ccggcttgat gtcattcttg gccaggtact ggagaaacgg
     1081 caacaggttt tcgccggcga tgatggaaag gaccttgacg gcctccaccc ggaaacgaat
     1141 ctcctccttc agcacggcct cactcaccac ggccggcgcc ggctcgttcc ccgagggacc
     1201 ttcggacggc acgccgccag gacccggtgc gtaggggtcc atcccgggcc gcacgatgga
     1261 agaaaccgcc cccgccgccg gctgcaccag cgggtaatcc tccttcaaac gctgtaccag
     1321 caatgtagcc gcctcgcggc cgcccagctg tccgaccatc aaggcatagt aggccacgta
     1381 atctttcagg ccatccggta ccgtcttgta ggtggtctcg actccgccgg gccagg
//
LOCUS       DAOQYN010000321         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_114405_length_1433_cov_7.052250, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000321 DAOQYN010000000
VERSION     DAOQYN010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114405_length_1433_cov_7.052250"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1433)
                     /locus_tag="PKN80_09290"
     CDS             complement(<1..>1433)
                     /locus_tag="PKN80_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNQ36240.1"
                     /translation="TDLNGKWWIFFDREDLGITTTQAPWFLADTRRRPARELTQEPPV
                     GHTEDLVSPDKTEFNRRFWARVQAEKRAIRIPSVWQELEPGYAGAAWYWRTFDCRGGR
                     KGQRAFLRFGAVAYFCEVWLNGRLAGRHEGGYLPFELEVTGRLQPGENRLALRVIDPD
                     ESRRVAGFALGGWKEREIPESKEAMWGLPFSGIWQGVSLERRPAVHLAGLFLRPEPAR
                     RRVTAEVSFRNPGEAVPGRLRLKISSWPDGKPAAPARTWDITVSQRDSRLVFELPFPK
                     FEYWSPEQPRLYRLEAELTPAGAEPDRLEERFGMREFTVRGDRFLLNGRPIFIRSTLY
                     QGNFPVGLAVPPDPNHCRRQVELAKGCGLNMLRVHVRPAHPDLLDAADELGMLLYEEP
                     AIAWMGDTPYLEERCRNEVAEMVRRDRNRPAVVLWGMINEGGGVAARLKGELSRLARE
                     LDPTRPVIDDSGGVQFGQPYLYLPGRDR"
BASE COUNT          197 a          525 c          502 g          209 t
ORIGIN      
        1 cggtcccggc cgggcaggta gaggtagggc tggccgaact ggacgccgcc ggagtcgtcg
       61 atgaccggcc gggtcgggtc cagttcccgg gccagccggc tcaactcgcc cttgagccgg
      121 gccgccaccc cgccgccctc gttgatcatc ccccagagca ccaccgccgg ccggttgcgg
      181 tcgcgccgga ccatttcggc cacctcgttg cggcagcgct cctccaggta gggggtgtcg
      241 cccatccagg cgatggccgg ctcctcgtag agcagcatgc ccagctcgtc ggcggcgtcc
      301 agcaggtccg ggtgggccgg ccgcacgtgc acccgcagca tgttcagccc gcagcccttc
      361 gccagctcca cctgccgtcg gcaatggttc gggtccggcg ggaccgccag cccgaccggg
      421 aagttgccct ggtagagagt ggagcggatg aagatgggcc ggccgttgag gaggaagcgg
      481 tcgccgcgga cggtgaactc gcgcatgccg aagcgctctt cgaggcggtc gggttccgcg
      541 ccggccggag tcagttcggc ctccagccgg tagaggcgcg gctgctccgg ggaccagtat
      601 tcaaacttcg ggaagggcag ctcaaatacc agcctgctgt cacgctggct tactgttatg
      661 tcccaggtcc gggccggcgc cgccggcttg ccgtccggcc aggagctgat tttcagccgc
      721 agccggcccg gcaccgcctc gcccgggttg cggaaggaga cctcggccgt cacccgccgc
      781 cgggccggct cgggccggag gaagaggccg gccaggtgca ccgccggccg ccgctccagc
      841 gacacgccct gccagatccc gctgaagggc aggccccaca tggcctcttt tgattcgggg
      901 atttcccgtt ccttccagcc gcccagggcg aagccggcca cgcgcctgct ctcgtccggg
      961 tcgatcaccc ggagcgccag ccggttttcg cccggttgca gtcggccggt cacctccagc
     1021 tcgaagggca ggtagccgcc ctcgtggcgg ccggccagcc ggccgttgag ccagacctcg
     1081 cagaagtagg ccaccgcccc gaaacgcagg aaggcccgct gacccttccg gccgccccgg
     1141 cagtcgaagg tgcgccagta ccaggccgcc ccggcgtagc cgggctccag ctcctgccag
     1201 accgagggga tccggatggc ccgcttctcg gcctggaccc gggcccagaa gcgccggttg
     1261 aactcggtct tgtcggggga gaccaggtcc tcggtgtggc cgaccggcgg ctcctgggtc
     1321 agctcccggg ccggccgccg ccgggtgtcg gccagaaacc agggcgcctg ggtggtggtg
     1381 atgcccaggt cctcccggtc aaagaaaatc caccacttgc cgttcaggtc cgt
//
LOCUS       DAOQYN010000322         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_117006_length_1414_cov_3.825607, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000322 DAOQYN010000000
VERSION     DAOQYN010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117006_length_1414_cov_3.825607"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..>1414)
                     /locus_tag="PKN80_09295"
     CDS             complement(181..>1414)
                     /locus_tag="PKN80_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36241.1"
                     /translation="MAKNERVQSVAGGRLEYLPFEALPLHDSVWGLCTGKDGMIYASA
                     CGECTGGLSVFILRYDPAARKLEYLLEVGPAVGQPPDNGHATQCKIHYCLLPGSDGLL
                     YCATHASGPPLGDPIWRPWNCWDDPVRNFPGSYLFTYDPASGRLDNFGIGPLKEGSRA
                     LALDEKRHKLYGLTWPRNHFYVYYLDQRRYLDLGRIGDINPQAIFLDRAGNAYTTDDY
                     GWILRCEAGRDRLQRLGVQCPSAAGRTGWHNVPYDVVPAPDWSCFYGTDWGFESRLWR
                     YDPYAGGDGRMDDLGPAFPLGSARTERSMEHWQVRGLVFGADGKLYYTMRLPEAAKDS
                     TWLLRLDPANGKREKVGRIRFGEHEPVAIASATPDFYGNLYFAEAGLSPTGLYIYRPT
                     GISSQPTTFSFKDIKPWG"
BASE COUNT          248 a          435 c          498 g          233 t
ORIGIN      
        1 cgcggcagag cgaggtgatg gccgattcac cctgaggaat cggctcggaa agggactggt
       61 ggttgcgcag gacccggttg atgatccgcc cctccctgac agaaggcgag tactccccgc
      121 ggtaggccgg cggtttgacc ggccaggttc gtttccgcaa atcttcttcc cggtacatgc
      181 tcacccccag ggtttgatgt ccttgaagga aaaggtggtc ggctggctcg aaatcccggt
      241 cggccggtag atgtacaggc cggtcggcga tagcccggcc tcggcaaagt aaaggttgcc
      301 gtaaaaatcg ggcgtggcgc tggcgatggc caccggttcg tgttcaccga acctgatccg
      361 gccgaccttc tcgcgcttgc cgttggcggg atcgagacga agcagccagg tggagtcctt
      421 cgccgcctcc ggaaggcgca tggtgtagta gagtttgccg tccgcgccga agaccaggcc
      481 ccggacctgc caatgctcca tgctccgctc ggtgcgtgcc gaaccgagcg ggaaagccgg
      541 gccgaggtca tccatccggc cgtcgccgcc ggcgtaggga tcgtaccgcc agaggcgcga
      601 ctcgaagccc cagtcggtcc cgtagaagca ggaccagtcc ggcgccggca ctacgtcgta
      661 agggacgttg tgccagccgg tccggccggc ggcgctggga cattggacgc ccaggcgctg
      721 aagccggtcg cggccagcct cgcagcggag tatccagccg tagtcgtcgg tggtgtaggc
      781 gttgccggcc cggtccagga agatcgcctg ggggttgatg tccccgatgc ggcccaggtc
      841 gaggtagcgg cgctgatcga ggtagtagac gtaaaaatgg ttgcgcggcc aggtcagtcc
      901 gtaaagtttg tgccgcttct cgtccagggc cagcgcccgg ctgccctcct tcaaggggcc
      961 gatgccgaaa ttgtcgaggc ggccggaggc cgggtcgtag gtaaagaggt aggaacccgg
     1021 gaagttccgg accgggtcgt cccagcaatt ccaggggcgc cagatcggat cgcccagcgg
     1081 cgggcccgag gcgtgggtgg cgcaataaag gaggccgtcc gaacccggca gcaggcagta
     1141 gtgaattttg cactgggtgg cgtggccgtt gtcgggcggc tgccccaccg cggggcccac
     1201 ctcgagcagg tactccagtt tccgggcggc cggatcgtag cgcaggataa agacgctgag
     1261 gccgccggtg cattccccgc aggcggaagc gtaaatcatc ccgtccttcc cggtacagag
     1321 accccagacc gagtcgtgaa gcggcagggc ctcgaaggga aggtactcca ggcgtccgcc
     1381 ggcaacggac tgaacccgct cattcttcgc cata
//
LOCUS       DAOQYN010000323         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_117283_length_1412_cov_3.477524, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000323 DAOQYN010000000
VERSION     DAOQYN010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117283_length_1412_cov_3.477524"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1412
                     /locus_tag="PKN80_09300"
     CDS             <1..>1412
                     /locus_tag="PKN80_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36242.1"
                     /translation="MVADNTYQLLVNAPGTYKVEGELQVRLAGDNLRFNPPPNTASVM
                     RLALPGDYDVSSPGVVKVVSRSAEATAVEVTFPPFGSVDVAWGPALREPTEAKVLASA
                     NLAWNLQPDLVRSSFSVRYEILYQPVKTLSIKLPAGVRVNNVEGNFIDWKEKGGLVEV
                     ELKPQTRGAVNLSVNYEQDLKSGQAEIELVSPEVPGVERTAGFATLSSPANLEVRLAG
                     QEGLVLVDPREAGAAGNAVMSFRYNRLPGRARLQLLRHEEVAVLEATADSANAISAVT
                     FDGQSVNRVIYNVRNNVKPFLRLKLPPGARLWSAHVGDSPVKPLAGQAGEILLPILRP
                     GARNVEFPVEVIYYLERPAFGRSGDLQLDLPVADIPVMQFMYSLYLPEKLKFESFGGS
                     LEKVASFTPIVLNEELEKVSGAAGPEGKEITRRSRLQDRYLAQQRALESNVAFSDELG
                     VREEAAAKPSTRGGKGYDSP"
BASE COUNT          240 a          466 c          491 g          215 t
ORIGIN      
        1 tcatggtcgc cgacaacacc taccagctgc tggtgaacgc gcccggcacc tacaaagtcg
       61 agggggagct gcaggtccgc ctcgccgggg acaacctccg tttcaacccg ccgcccaaca
      121 ccgcctcggt catgcgcctg gccctgccgg gcgattacga tgtctcgagt ccgggcgtgg
      181 tgaaggtcgt ctctcggagc gcggaggcaa ccgccgtcga agtcaccttt ccgcccttcg
      241 gttcggtgga cgtcgcctgg ggtccggcgc tgcgcgagcc gaccgaggcc aaggtgctgg
      301 ccagcgccaa cctggcctgg aacctccagc cggacctggt gcggagcagc ttctcggtcc
      361 gctacgagat cctctaccag ccggtgaaga cgctttcgat aaagctgccg gccggggtcc
      421 gggtcaacaa tgtcgagggg aacttcatcg actggaagga gaagggcggc ctggtcgagg
      481 tggaactgaa gccgcagacc aggggggcgg tcaacctgtc ggtgaattac gagcaggacc
      541 tgaagtcggg gcaggctgag atcgagctgg tcagcccgga ggtacccggc gtggagcgga
      601 ccgccggctt tgccacgctc tccagcccgg ccaacctgga ggtcaggctg gccggccagg
      661 agggtctggt cctggttgac ccgcgggagg ccggcgcggc cggcaacgcc gtcatgagtt
      721 tccgctacaa ccggctgccc ggccgggccc ggctgcagct gctgcgccac gaggaggtgg
      781 cggtcctgga ggccaccgcc gactcggcca acgccatctc ggcggtcacc ttcgacggcc
      841 agagcgtcaa ccgggtcatc tacaacgtcc ggaacaacgt caagcccttt ctccgcctga
      901 agctgccccc gggggcccgg ctctggagcg cccacgtcgg cgacagcccg gtcaagcccc
      961 tggccggcca ggcgggggag atcctgctgc cgatcctgag gccgggcgcc cggaacgtcg
     1021 agttcccggt cgaggtcatc tactacctgg agcggcccgc tttcggacgc agcggcgacc
     1081 tgcagcttga cctgcccgtg gccgacatcc cggtgatgca gttcatgtac tcgctctacc
     1141 tgccggagaa gttgaagttc gagagcttcg gcggcagcct ggagaaggtg gcctccttca
     1201 ccccgatcgt gctgaatgag gagctggaga aggtttccgg cgctgccggg ccggaaggga
     1261 aggaaataac ccgccggagc cggctgcagg accgctacct ggcccagcag cgggcgctgg
     1321 aatcaaatgt cgccttctcc gatgaactgg gggtccgtga agaagcggcg gcaaaaccgt
     1381 cgacccgggg cggcaagggg tatgacagcc cg
//
LOCUS       DAOQYN010000324         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_117858_length_1408_cov_2.755358, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000324 DAOQYN010000000
VERSION     DAOQYN010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117858_length_1408_cov_2.755358"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKN80_09305"
     CDS             complement(<1..392)
                     /locus_tag="PKN80_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNQ36243.1"
                     /translation="MKLRELFEGLPVKRYLGSDNPEITGLAVDSRKVRPGYLYLAVVG
                     SRDDGHRYLSDAIERGARAVVLQKESEAAGGVPTVLVETTRRLLPEVAGRFFRFPARK
                     LRIVGITGTNGKTTTSYLIESVLAAAGR"
     gene            complement(500..>1408)
                     /locus_tag="PKN80_09310"
     CDS             complement(500..>1408)
                     /locus_tag="PKN80_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNQ36244.1"
                     /translation="RNRAITDINEPGSVFKMITAAGVLEEGAVKPSDRFFCENGAWRV
                     PGRSNPVHDAHPYGSLTFREIIVKSSNIGTSKAALKMGKERMHHYAASFGFGKRTGID
                     LPGEVRGILHPLANWSGVSLYAIPYGQEVAATAIQSTAAMAAICNGGTLNRPYLVSRI
                     STREGKTVEKFQPKAVRRIISEKTCGILLPILSEVTSEEGTARWADIPGYLVGGKTGT
                     GQKVEGGHYVKKYFSSFIGFINTGQKKFAVFIGADEPHNGYYGGVVAGPAFNRIGQRA
                     LITLNIPPDQAEVDTAPFLPKTGPKR"
BASE COUNT          221 a          390 c          519 g          278 t
ORIGIN      
        1 ttgcggccgg cggcggccag gacgctctcg atcaggtaac tggtggtggt cttgccgttg
       61 gtgccggtga tgccgacgat ccgaagcttg cgggccggga aacggaagaa gcggccggct
      121 acttcgggga ggaggcgccg ggtggtttcg accagcacgg tcggcacgcc gccggccgct
      181 tcggactcct tctggaggac gaccgcccgg gcgccccgtt caatggcgtc ggacaggtag
      241 cggtggccgt cgtcccggga gccgaccacg gccaggtaaa ggtagccggg tcggaccttg
      301 cggctgtcga ccgccaggcc ggtgatctcc gggttgtcgg agcccaggta ccgcttgacc
      361 ggaagtcctt cgaagagttc gcgtagcttc atcgtgtttt cagggaacaa cgttcatcct
      421 aatattttac tttatggcgg ggattaagac aacggtccgg aagggatcgg gagaactgtt
      481 cgccggccgg cggtcaggct taccttttcg ggccggtttt gggtaggaac ggagcggtgt
      541 caacctcggc ctggtcgggt ggaatgttca gcgtgatcag ggcgcgctgg ccgatgcggt
      601 tgaaggccgg accggccacg acgccgccgt agtagccgtt gtggggttcg tccgcgccga
      661 tgaagacggc gaatttcttc tgaccggtgt tgatgaagcc gatgaaggac gagaagtatt
      721 ttttgacgta gtggcccccc tcgaccttct ggccggtgcc ggtcttgccg ccgaccaggt
      781 agccgggaat gtcggcccag cgggcggtgc cctcctcgga ggtgacttcg gagagaatgg
      841 gcagcaggat gccgcaggtc ttttcggaga tgatccggcg gacggctttg ggctggaact
      901 tctcgacggt tttgccttcc cgggtggaga tccggctgac caggtacggc cggttcaggg
      961 tgccgccatt gcagatggcg gccatggcgg cggtcgactg gatggcggtg gccgcgacct
     1021 cctggccgta gggaatggca tacagggaga cgcccgacca gttcgcgagc ggatgcagga
     1081 tgccccgcac ttcgccgggc aggtcgatgc cggtcctttt gccgaaaccg aaggaggcgg
     1141 cgtaatggtg catgcgttcc ttgcccatct tcagggcggc cttggaggta ccgatgttgc
     1201 tgctcttgac gatgatctcc cggaaggtga ggctgccgta agggtgggcg tcgtggaccg
     1261 ggttggagcg gccgggaacc cgccaggcgc cgttttcgca gaagaaacgg tcggatggct
     1321 tgaccgcacc ttcctccagg acgccggccg cggtgatcat cttgaagacc gagccgggtt
     1381 cgttgatgtc ggtgatggcc cggttgcg
//
LOCUS       DAOQYN010000325         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_118190_length_1406_cov_2.095485, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000325 DAOQYN010000000
VERSION     DAOQYN010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118190_length_1406_cov_2.095485"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1406)
                     /locus_tag="PKN80_09315"
     CDS             complement(<1..>1406)
                     /locus_tag="PKN80_09315"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ36245.1"
                     /translation="LHRGGGLAGVCGALAARRHGLKTALIQDRPVLGGNASSEVRVNI
                     SGAAGKNPGGRETGIICELFLAERKINSAPYSNSFINSNLGLVLYDAVKSSGIDLFLN
                     TSARRVRVEERRIISVACCQLGSEKELLFKGDLFLDASGDGTVAAAAGARFRFGREGR
                     AEFGEIFAPPRPDRGVMGSSLLFLARDAGRPVPYHPPAWAARYRPGDPALKTRAHYHG
                     YHSARPVLGGFWWIEIGYPWDTVGENETIREKLLAHLLGVWDHLKNGGEHGFGNFVLE
                     WVGMLPGKRESRRFEGDYLLRESDLLERRYFPDAIGYGGWWIDQHTPGGILARREPPI
                     VRVVENQRRDQAEVSPYQIPFRSLYSRDIRNLLLAGRNISVTHVALSSTRVMGTCGLL
                     GQASGTAAWLCRKYRLEPREIHPARIKELQQVLLRDGVFIPGVVNEEKGDLLRGARAR
                     ASSQAPLGLPTSEGEWEL"
BASE COUNT          256 a          470 c          448 g          232 t
ORIGIN      
        1 tcaagttccc attccccctc agaagtcggc agccccagcg gcgcctggga ggaggccctt
       61 gcccgggctc cccgcagcag gtcgcccttc tcctcgttga caaccccggg aatgaagacg
      121 ccgtccctca ggaggacctg ctgcagttct ttgatccggg ccgggtggat ctcccggggt
      181 tcgagccggt acttgcggca gagccaggcg gccgtcccgg aggcctggcc aagcaggccg
      241 caggtcccca tgacccgggt gctgctcagg gccacgtggg tgacgctgat gttccggccg
      301 gccaggagca ggttacggat atccctggaa taaagagacc ggaaaggtat ctgatagggc
      361 gaaacctcgg cctggtcgcg gcgttgattt tcaaccaccc ggactatggg cggttcccgg
      421 cgggccagga ttccgcccgg cgtgtgctga tcaatccacc agccgccgta gccgatcgcg
      481 tccgggaaat accgacgttc gagcaggtcg gactccctca gcaggtaatc cccctcgaag
      541 cggcgcgact cccgtttgcc gggcagcatt cccacccact ccaggacaaa attcccgaac
      601 ccgtgttcac cgccattctt aaggtggtcc cagactccga gtaggtgggc cagcagcttc
      661 tcccggattg tttcgttctc gccgacggta tcccagggat acccgatttc tatccaccag
      721 aaaccgccca gaaccggccg ggccgaatgg taaccatggt agtgcgcgcg ggtcttgagg
      781 gccgggtcgc cgggccggta gcgggcggcc caggcgggcg gatggtaggg gaccggccgg
      841 ccggcatccc gggccaggaa aagcagggaa ctgcccatca cgccgcggtc cggccggggg
      901 ggcgcaaaaa tctccccgaa ctcggcgcgc ccctcgcgtc cgaaacggaa ccgggcgccg
      961 gcggcggccg ccaccgtgcc gtcgcccgag gcgtccagaa agaggtcacc cttgaagagt
     1021 aactccttct cgctgcccag ctggcagcag gcgactgaaa ttatacgccg ctcttcaacc
     1081 cggacccggc gggcgctggt gttcagaaag agatcgatcc ccgagctctt taccgcgtcg
     1141 taaaggacca gacccaggtt ggaattgatg aaggaatttg agtaaggcgc ggagttgatt
     1201 ttccgctcgg ccaggaaaag ttcgcagatg atcccggtct cccggccgcc tgggtttttt
     1261 ccggccgcgc cggagatatt gaccctgacc tctgaactgg cattgccgcc caaaaccggc
     1321 cgatcctgaa tcaaggccgt cttcaaaccg tggcggcggg ccgccagggc cccgcaaact
     1381 ccggccagac cgccgccgcg atggag
//
LOCUS       DAOQYN010000326         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_118825_length_1401_cov_3.912333, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000326 DAOQYN010000000
VERSION     DAOQYN010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118825_length_1401_cov_3.912333"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PKN80_09320"
     CDS             <1..184
                     /locus_tag="PKN80_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36246.1"
                     /translation="TPPSSAAPPGSAVSGSRARATVYCLGRSLHAGQRRRLEVELVRE
                     NGWWKTRLGLRDSPAD"
     gene            complement(222..515)
                     /locus_tag="PKN80_09325"
     CDS             complement(222..515)
                     /locus_tag="PKN80_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36247.1"
                     /translation="MPDCYFHTGVPTSDHCQQCGRPLCRRCGIRNEHGVFCSESCCSS
                     FISFSRKDEEIKRLLRKRRRPVAAFFSGLLQILLAAAVIYGIYRGFLFIRSFL"
     gene            complement(572..>1401)
                     /locus_tag="PKN80_09330"
     CDS             complement(572..>1401)
                     /locus_tag="PKN80_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNQ36248.1"
                     /translation="ELEGGKPLAVIGASVKKTLFLAENPVGKFVKVKSSYFRVIGVTG
                     EKSVSGAGSQFAAEDINFDIYIPMSTERSIFGEYEIAKGSGNLFDRERNLVKFHRFVA
                     RVSEMDKIVDTSTIINGILNTTHKKADYEILVPLQLLQQAEHTTRIFNIVLGSIAAIS
                     LLVGGIGIMNIMLATVTERTREIGIRRALGARRRDIINQFLSESVILSALGGLMGVFL
                     GVAVPSLVTRFAGMATIVSVWSILVAFSISVGVGILFGIYPARKAAMLDPIQALRYE"
BASE COUNT          261 a          404 c          476 g          260 t
ORIGIN      
        1 tacgccgcct tcgagcgcgg cgccaccggg atcagcggtg agcgggtcga gggcaagggc
       61 tacggtttac tgcctggggc gcagtctgca tgccggccag cgccggcggc tggaggtgga
      121 actggtcagg gagaatggct ggtggaagac gcgcctcggc ctgcgcgatt ccccggccga
      181 ttaggaccgt cggctggacg ctcccgcctc gccgcggttt cctaaagaaa ggaccggata
      241 aagaggaagc cccggtaaat cccgtagatg acggccgccg ccagcaggat ttgcagcagg
      301 ccgctgaaaa aggcggccac cggccggcgt cgttttctca gcaggcgctt gatttcctca
      361 tctttccggg agaaggagat gaaggaactg cagcagcttt ccgagcagaa gacgccgtgt
      421 tcgttgcgga tcccgcatct tcggcagagg gggcgtccgc actgctggca gtggtcggaa
      481 gtcgggactc cggtatgaaa gtagcagtca ggcatgccta aattctaatc ccgcccgccc
      541 cgtaaagtca aaccgccttt atcaagttgt cttattcgta gcggagggcc tggataggat
      601 ccagcatggc ggccttccgg gccgggtaga tgccgaagag gatgccgacg cccaccgaga
      661 tggagaaggc caccaggatc gaccagacac tgacgatggt ggccatgccg gcgaagcggg
      721 tgaccagact gggcaccgcc acccccagga agacgcccat caggccgccg agggccgaca
      781 ggataaccga ctcggagagg aactggttga tgatgtcccg gcgccgggcg cccagggccc
      841 tgcggatgcc gatctcccgg gtgcgctcgg tgacggtggc cagcatgatg ttcatgatgc
      901 cgatgccgcc gaccagcagg gagatggcgg cgatcgagcc caggacgatg ttgaagatgc
      961 gggtggtgtg ctcggcctgc tggagcagct gcaggggcac caggatttcg tagtcggcct
     1021 tcttgtgggt ggtattgagg attccgttga tgatggtcga ggtgtccacg atcttgtcca
     1081 tttcactcac cctggccacg aagcggtgga acttgaccag gttgcgctcg cggtcgaaga
     1141 ggttgccgct gcccttggcg atttcgtact cgccgaagat ggagcgttcg gtgctcatcg
     1201 ggatgtagat gtcgaagttg atatcctcgg cagcgaactg gctgccggcc ccggagaccg
     1261 atttttcgcc ggttaccccg atgacccgga agtagctgct cttcaccttg acgaatttcc
     1321 cgaccgggtt ctcggccagg aagagcgtct ttttgaccga ggcgccgatg accgccagcg
     1381 gcttgccgcc ttccagttcg a
//
LOCUS       DAOQYN010000327         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_118834_length_1401_cov_3.424963, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000327 DAOQYN010000000
VERSION     DAOQYN010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118834_length_1401_cov_3.424963"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PKN80_09335"
     CDS             <1..458
                     /locus_tag="PKN80_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HNQ36249.1"
                     /translation="VRLAIAGNEAFTFSEIELGRSGPSFTYETVQALLSEYGPGHEWH
                     LILGLDAFREIGTWHRLAELVQLVQFAVADRPGFRPPEAVPPGVRYQRLELGLFPVSG
                     REIRERAARGESIRYLVTDAVYRYINSRRLYRPAEVRPETAAPKTGKGR"
     gene            458..1267
                     /locus_tag="PKN80_09340"
     CDS             458..1267
                     /locus_tag="PKN80_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ36250.1"
                     /translation="MLKLTFMTWLCPNWTIKEIIAGALKYGYQGVEFRVQANQAHGVE
                     LGMDPARIKSIRQDFAGAGLETPCLATSLQFAIADAARRAENVQLLRQYLELAQALGV
                     PYIRVFAGLPECEAAGAIERMNESLQQAVAAAGAPSVGVLLETHDWCSVSLRVARLLE
                     KVANPRINALWDIMHPYRELETPAETYGRIGRWVKHLHIHDGIYREERTRIEICPLGD
                     GEVPHREPMALLKKAGFTGHFAIEIIRRNDPQPEFFIEQYARKFREYEAAL"
BASE COUNT          240 a          465 c          490 g          206 t
ORIGIN      
        1 tggtccggct ggccatcgcc ggcaacgagg ccttcacctt ttcggagata gaactcggcc
       61 ggagcggccc ttccttcacc tacgaaacgg tccaggcgct gctctccgaa tacgggcccg
      121 gccacgagtg gcacctcatt ctcggcctgg acgccttccg cgagataggt acctggcacc
      181 ggctggcgga gctggtccag ctggtccagt tcgcggtggc cgaccggccc ggcttccggc
      241 cgccggaggc agtgccgccg ggagtccgtt accagcggct ggaactgggc cttttcccgg
      301 tttccggacg ggagatccgg gagcgggcgg cccggggcga atcgatccgc tacctggtca
      361 ccgacgcagt ttaccgatac atcaacagcc gccggcttta ccggccggcc gaagtccggc
      421 cggagacggc ggcgccaaag acgggaaagg ggagataatg ctgaaactga cgttcatgac
      481 ctggctctgt ccgaactgga cgatcaagga gatcatcgcc ggcgccctca agtacggtta
      541 ccagggtgtc gagttccggg tgcaggccaa ccaggcgcac ggcgtggaac tgggcatgga
      601 tccggcccgg atcaagtcga tccgtcagga cttcgccggg gccggcctgg agacgccctg
      661 cctggccacc tcgctccagt tcgccatcgc cgatgcggcc cggcgggccg agaacgtcca
      721 gctgctccgc cagtacctgg agctggccca ggcgctgggc gtaccttaca tccgggtctt
      781 cgccggcctg cccgagtgcg aggcggccgg ggcgatcgag cgcatgaacg agagcctgca
      841 gcaggcggtg gccgcggccg gcgcgccctc ggtcggcgtg ctcctggaga cgcacgactg
      901 gtgcagcgtc agcctgcggg tggcccggct gctggagaag gtggccaacc cgcggatcaa
      961 cgccctctgg gacatcatgc acccctaccg ggagctggag acgccggccg agacctacgg
     1021 ccggatcggc cgctgggtca aacaccttca cattcacgac ggcatttacc gggaggagcg
     1081 gacccggatc gagatctgcc cgctgggcga cggcgaggtg ccgcaccggg aaccgatggc
     1141 cctgctgaag aaggccgggt tcaccggcca cttcgcgatc gagatcatcc gcagaaacga
     1201 tccccagccg gaattcttca tcgagcagta cgcccggaag ttccgggagt acgaggcggc
     1261 cctttaaccg gaggcgggga tggcgatcag gaccgcgatc atcggctacg ggaagagcgg
     1321 cagccggctg cacgccgacg ggctggcgca ggtgccggaa ttcaaggtga cggccgtctg
     1381 cgacccggac ccggaacggc g
//
LOCUS       DAOQYN010000328         1382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_121536_length_1382_cov_3.360965, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000328 DAOQYN010000000
VERSION     DAOQYN010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121536_length_1382_cov_3.360965"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..>1382)
                     /locus_tag="PKN80_09345"
     CDS             complement(21..>1382)
                     /locus_tag="PKN80_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ36251.1"
                     /translation="DAAAITVILVIIFNMHVKMSLVALTVFPIYLYFFWKLGREVRAS
                     SYEVQKETAVLSGSVSERISGSQVIHAFTKELSERKHFYRDSRSLYARTMQTVYFQSL
                     NMAISGVLTQLAPLLVIAYGGHLVITREITIGQLVAFMLYLNPLYIPLQRFSELNAIF
                     ANSMAALDRIFEILDEKPEVVDKPGAVILKSVEGRVEFDRVGFSYKPKEPVLSGVSFL
                     VKPGQRVALVGPSGSGKSTLVSLLPRFYDVDSGRISIDGHDLRTIQVKSLRRHIGLVL
                     QDPVLFSGSIRENILYGNPESGWEEMVAASRAANAYDFISELPEGFETDVGERGNFLS
                     GGQKQRLTIARAFLKDPKILVLDEPTSALDAESEQLIKEALERLMVNRTTFIIAHRLS
                     TVSNVDFILVLYQGRIVESGRHEELLRNRGLYHGLYSQQFGYIPPETEETGNGKAGVA
                     GLA"
BASE COUNT          264 a          434 c          432 g          252 t
ORIGIN      
        1 ggggagcggc ctcagccgcc tcaagccagt ccagcaactc cggcttttcc attgccggtc
       61 tcctcggtct cgggcggaat ataaccaaac tgctggctgt aaaggccgtg atagagcccg
      121 cggttgcgca gcagctcctc gtggcggccg gactccacga tccgaccctg gtaaaggacc
      181 aggatgaaat caacgttgct caccgtggaa aggcggtggg cgatgatgaa ggtggtccgg
      241 ttgaccatca gccgttccag ggcctccttg atcagctgct ccgactcggc gtccagggcc
      301 gaggtgggct catccaggac caggatcttc ggatccttga ggaaggcccg ggcgatggtt
      361 aagcgctgct tctggccgcc ggagagaaag ttgccccgtt cgccgacatc ggtctcgaaa
      421 ccctcgggca gctcgctgat gaagtcgtag gcattggcgg cccggctggc cgccaccatc
      481 tcctcccagc cggactccgg gttcccgtac aggatgttct cccggatgct gccgctgaaa
      541 agaaccgggt cctggagcac caggccgatg tggcggcgca gtgacttcac ctggatcgtc
      601 cgcaggtcgt gcccgtcaat cgagatgcgg ccggaatcga cgtcgtaaaa acgcggcagc
      661 aggcttacca gcgtgctctt gcccgaaccg ctcggtccga ccagggccac ccgctggccg
      721 ggctttacca gaaacgagac accggacagg accggctcct tcggcttgta ggagaagccg
      781 acccggtcga actcgacccg tccctcgacc gacttcagga tcacggcgcc cggcttgtcg
      841 accacctccg gcttctcgtc caggatctca aagatccggt ccagcgcggc catggaattg
      901 gcgaagatcg cgttcagttc cgaaaaacgc tgcagcggga tgtaaagcgg gttgagatag
      961 agcatgaagg ccaccagctg accgatggtg atctcccggg tgatcaccag gtgcccgccg
     1021 taggcgatca ccagcagcgg ggccagctgg gtcagcaccc cgctgatggc catgttcagg
     1081 ctctggaagt aaacggtttg catggtccgg gcgtagaggc tccggctgtc gcggtagaaa
     1141 tgcttgcgct cgctcagctc cttggtaaag gcgtggatta cctggctccc ggaaatgcgc
     1201 tcgctgacgc tgccggaaag caccgccgtc tccttctgca cctcgtaact gctggcccgc
     1261 acctcccgtc ccagtttcca gaaaaagtag aggtagatcg ggaagaccgt cagggcgacc
     1321 aggctcatct tgacgtgcat gttgaagatg atgaccagga tcaccgtgat ggccgccgcg
     1381 tc
//
LOCUS       DAOQYN010000329         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_122129_length_1378_cov_3.086168, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000329 DAOQYN010000000
VERSION     DAOQYN010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122129_length_1378_cov_3.086168"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKN80_09350"
     CDS             complement(<1..469)
                     /locus_tag="PKN80_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNQ36252.1"
                     /translation="MGMIQSVSGARGRVGEDFTAEACFKLAAAFGNFLPKGPVLIGRD
                     SRPSGKTARQSVAAGLLLTGHQVVDLGLVPTPAVGFAVPVLKARGAVVITASHNPAEW
                     NGLKFLDDRGLYLGPESLRSVYAPDRTTDPAGRGSGDESVPADILNDYLDAVVE"
     gene            628..>1378
                     /locus_tag="PKN80_09355"
     CDS             628..>1378
                     /locus_tag="PKN80_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36253.1"
                     /translation="MRINILATLGRRPGGAHRNFLEYADRLTGRGHRVTVIKAINRAG
                     TRPAGFAARGREWIFLHTGAPRRPYRVAWLKCSAEIVAVPSYREEYLPAADATLFSST
                     RLIETVAGFSERNGAKLMRVADIFFARQPPEVPAEIQLVATSSLVRNLLLKRFPERKI
                     PLLLNGVNLELFRNPDRRFRPARRIGFLFYQKRPAHKGLDDAVAALEKLRSEFPGLEF
                     ACAGFRRESWLPGWIEFFAGQDQAALARFYSD"
BASE COUNT          245 a          465 c          411 g          257 t
ORIGIN      
        1 gttcaaccac ggcgtcaagg taatcgttca ggatatcggc cggaacggac tcgtccccgc
       61 ttccccggcc ggctggatcg gtggttcgat ccggggcata aaccgaacgc agggactccg
      121 gccccaggta aagcccccgg tcatccagga acttcaggcc gttccactcg gccggattat
      181 ggctggcggt gatcaccacc gcgccccggg ctttcagaac cggcacggca aaacccaccg
      241 ccggagtcgg caccagcccg agatcaacca cctggtggcc ggttaagagt aaccccgcgg
      301 ccacggactg ccgggccgtt tttccggaag gccggctgtc gcggccgatc aaaaccggtc
      361 ccttcggaag gaaatttccg aaggcggccg ccagtttaaa acaggcctcg gcggtaaaat
      421 cttcgcccac ccggccgcgg gccccggaaa cgctctggat catgcccatc ccgggtttgc
      481 ctccgacggc cgcgccccaa gagaaccttg cgggtttggt gaattttcgc gttttgattc
      541 cgttcgtttc atgtcggctt ccttttatat tataataaca taatcaaaat cggaagggtc
      601 ggaatcgttt cttgggaaac cggggctatg cgcatcaaca ttctggccac gctcggccgg
      661 cggccgggcg gggcacaccg caactttctg gagtatgccg accgtctcac cggacggggc
      721 caccgggtta ccgtcatcaa ggcgatcaat cgcgccggca cccggccggc cggattcgcg
      781 gcccgcgggc gcgagtggat ttttctgcac accggcgcgc cccggcggcc gtaccgggtt
      841 gcctggctta agtgttccgc cgaaattgtg gccgtgccgt cctaccggga agaatatctg
      901 ccggcggccg acgccaccct gttcagctca acccggctga tcgaaaccgt ggccggcttc
      961 tccgagcgca acggagcgaa actgatgagg gtggccgata ttttcttcgc ccggcagccg
     1021 ccggaggtac cggctgaaat ccagctggtc gccacctcgt cgctggtcag gaatctgctg
     1081 ctcaagcgct tcccggagcg aaaaatcccc ctgctgctca acggggtcaa tctggaactc
     1141 ttccggaacc cggaccggcg tttccgtccg gcccgccgga tcggcttcct cttctatcaa
     1201 aaacgcccgg cccacaaggg ccttgatgac gcggtggccg ccctcgaaaa gctgcgttcc
     1261 gaattccccg gcctggaatt cgcctgcgcc ggtttccggc gcgaaagctg gctgcccggc
     1321 tggatagaat tcttcgccgg ccaggaccag gcggccctgg cccgcttcta ttccgacc
//
LOCUS       DAOQYN010000330         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_125440_length_1356_cov_3.089162, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000330 DAOQYN010000000
VERSION     DAOQYN010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125440_length_1356_cov_3.089162"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..329)
                     /locus_tag="PKN80_09360"
     CDS             complement(54..329)
                     /locus_tag="PKN80_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNQ36254.1"
                     /translation="MRRVTIVKAVADKTGLEQKLVARIIKEFLAETETALLAGQPVQL
                     RPLGTLTPRRRRAKTGRNPFTNQPIPVPEHLTLVFKPSRRIKKRLNQ"
     gene            650..976
                     /gene="rplU"
                     /locus_tag="PKN80_09365"
     CDS             650..976
                     /gene="rplU"
                     /locus_tag="PKN80_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNQ36255.1"
                     /translation="MFAIIQTGGKQYQVTPGARLKVEKLAGAAGEKVVFEKVLLFSTD
                     DGAVVTEVTRLSDIKVKGRIVEQGRARKVVVFKRKPKTDYNKLQGHRQGYTLVEIEEI
                     SKGKKA"
     gene            978..>1356
                     /locus_tag="PKN80_09370"
     CDS             978..>1356
                     /locus_tag="PKN80_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNQ36256.1"
                     /translation="MNRAFLLVLDEGPDGFPAEAARVELESLCRTAGLQAAAGEFLRV
                     REPNPSTYLGSGRVAEFAGRVKDSGFETVVLNVDLSAVQQRNLEEAWGIPVLDRTSLI
                     LRIFSFHANSAEGKLQVDLARENY"
BASE COUNT          269 a          378 c          438 g          271 t
ORIGIN      
        1 gaactggccg gggcactcgc ggatcggatc gcccagggcc agccagatgg ccttcattga
       61 ttaagtcttt ttttgatgcg ccggctcggt ttaaagacca gggtcaggtg ctccgggacc
      121 ggtatcggct ggttggtaaa gggattgcgt ccggtcttgg cccgacgccg tcggggcgtc
      181 agggttccca gcggccgcag ctggaccggc tgcccggcca gcagggccgt ttcggtttcg
      241 gccaggaact ccttgatgat ccgggccacc agcttctgtt ccagcccggt cttatccgcc
      301 accgccttta cgatcgtgac ccggcgcatt cttccgtctc ccccaggaat agtagatcag
      361 cttccgcccg tccggcttca ataaaatccg tttccggaag gtggcccgga ccccgggaaa
      421 ttccggttct tttttcgcct cggcaagccg ggcaacgcgg tccgggttta ctttttcggc
      481 catttgggat aagatatata attaaaaaaa ggaagcgttt caaccaccgg gtattataga
      541 acaaccgggg gataaaaaac aaggccggta acggcctgga tcaaaggtgg gccggcagac
      601 gggcattctc tcggcggccc gtcccgtttc tttcgaagag ggaggcgaga tgttcgcgat
      661 catccaaacc ggcggtaagc agtaccaggt gacaccaggc gcccggctca aggttgagaa
      721 gctggccggc gcggccgggg agaaggtggt ttttgaaaag gtgcttctct tttccaccga
      781 tgacggggcg gttgtgaccg aagtaacccg cctttccgac atcaaggtca aggggcggat
      841 cgtggaacag ggcagggcca ggaaggtggt ggtcttcaag cgcaagccga agaccgatta
      901 caacaagctc caggggcacc gccagggcta cacgctggtt gagatcgagg aaataagtaa
      961 gggaaagaag gcttgaattg aaccgggcct tcctgctggt gctggacgag gggccggacg
     1021 gtttcccggc cgaagcggcc cgggtggagt tggaaagcct ctgccggacg gccggactgc
     1081 aggcggccgc cggggagttt ctaagggtcc gggaaccaaa cccctccacc taccttggct
     1141 cgggacgggt agcggagttc gccgggcggg tgaaggattc cggatttgaa accgtcgtct
     1201 tgaatgttga tctttcggcc gttcagcagc gcaacctgga agaggcctgg ggtattccgg
     1261 tgctggaccg gacctcgctc atcctccgca tcttctcttt ccacgccaac agcgctgagg
     1321 gaaaactcca ggtcgacctg gcgcgtgaga attacc
//
LOCUS       DAOQYN010000331         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_132390_length_1314_cov_3.964257, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000331 DAOQYN010000000
VERSION     DAOQYN010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132390_length_1314_cov_3.964257"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1314)
                     /locus_tag="PKN80_09375"
     CDS             complement(<1..>1314)
                     /locus_tag="PKN80_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNQ36257.1"
                     /translation="LFTWTEPKELLLQSRNYGPAPAAENLTLTIEDYFGREAWRQSIR
                     AGAGAGTTAAVKIGLPDHLRGSLRALLKDGSGQVLNEQIFAVVPPPRYPGLRPESKFG
                     ALVQFSDANLYLARALGTKWSRDHWSFCWYAVEPKPGEWTWEKADEKLDRALKYGVSV
                     FGVLHGTPAWASEDGSQGYTSRPRNWSEWETYVEKTVGHFKGRVRVWEVWNEPQSADF
                     YAELCRRTYAAAKRADPDCLVIGLSSTLFSGGFLRRFVELGGLNHLDEVSAHFYNYSS
                     GLAEAFKKYSQLLGGKPVWNTEGGGWGGSTFYSTRPDYRPERPGVQQVGHFYTTVCSL
                     PEARFQCYYWNTWPADYNPGFEYSWTFIEHDSSLKPEGSAFAVTSYSLDGARPVKILS
                     EDALKIHLFERGSSALAAFWWEEKGELDLELKFPRPAGGLVRDIMG"
BASE COUNT          210 a          436 c          453 g          215 t
ORIGIN      
        1 cccatgatgt cgcgcaccag gccgccggcc ggcctcggaa acttcagttc caggtccagt
       61 tcacccttct cctcccacca gaaggcggcc agggccgagc tgccccgttc gaagaggtgt
      121 atcttcaggg cgtcctcgga gaggatcttg accggccggg ctccgtccag cgagtaggaa
      181 gtgaccgcga aggcgctgcc ctcgggcttg aggctggaat cgtgctcgat gaaggtccag
      241 gagtactcga agccgggatt gtagtcggcc ggccaggtat tccagtagta gcactggaaa
      301 cgggcctccg gcagcgagca gaccgtggtg tagaagtggc ccacctgctg gacgccgggc
      361 cgctcgggcc ggtaatcggg ccgggtggag taaaaggtgg agccgcccca gccgccgccc
      421 tcggtattcc agaccggctt gccgcccagc agctggctgt actttttgaa ggcttcggcc
      481 aggccggagg agtagttgta gaagtgggcc gagacctcgt ccaggtggtt gagtccgccc
      541 agttcgacga agcggcgcag gaagccgccg gagaagaggg tcgaggagag gccgatgacc
      601 aggcagtccg gatcggcccg cttggcggcc gcgtaagtgc ggcggcagag ttcggcgtag
      661 aaatcggccg actggggctc gttccagacc tcccagaccc ggacccggcc cttgaaatgg
      721 ccgacggtct tttcgacgta ggtctcccac tcggaccagt tcctgggccg gctggtgtag
      781 ccctgggagc cgtcctcgct cgcccaggcc ggcgttccgt gcaggacccc gaagacgctc
      841 accccgtact tgagcgcccg gtccagcttc tcgtcggcct tctcccaggt ccactcgccc
      901 ggcttcggtt ccaccgcgta ccagcagaag gaccagtggt cccggctcca cttggtgccc
      961 agcgcccggg ccaggtaaag attggcgtcg ctgaactgga ccagggcccc gaacttcgat
     1021 tccggccgca ggcccgggta gcggggcggc ggcaccaccg cgaagatctg ctcgttgagg
     1081 acctggccgg acccgtcctt cagcagggcc cgcaggctgc cgcgcaggtg gtcgggcagg
     1141 ccgatcttaa ccgcggcggt cgtcccggcc ccggccccgg cccggatcga ctggcgccag
     1201 gcctcccggc cgaagtaatc ctcgatggtc agggtcaggt tctcggcggc cggcgccggc
     1261 ccgtaattac ggctctggag aagcagctcc ttcggttccg tccaggtgaa gagg
//
LOCUS       DAOQYN010000332         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_133450_length_1308_cov_3.543496, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000332 DAOQYN010000000
VERSION     DAOQYN010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133450_length_1308_cov_3.543496"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..765
                     /locus_tag="PKN80_09380"
     CDS             178..765
                     /locus_tag="PKN80_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36258.1"
                     /translation="MRIFNRLVAWIYSLASLYVSVIFILIWLDPNFTNYFCERIHAIE
                     PYKFALVGGALLMLGAIWLASVIEHHYRTRSVSFNGPGGLVRVQLSAVEDFLMNEVRQ
                     HLKAVKRIRTSTLMTPKGLQVFNQLVIWSGYDIPTVTGKVQDLIKRLLEDTIGVERVG
                     DIRISVSRISAGESGGPVEEDEDEEELAGEDTEIH"
     gene            725..>1308
                     /locus_tag="PKN80_09385"
     CDS             725..>1308
                     /locus_tag="PKN80_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002743139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNQ36259.1"
                     /translation="MKKNWPEKIRKSIEEAVANYRRLSGPELSERLAQLAELVTETIA
                     GGHRVYFCGNGGSAADAQHLAAEFVGRFRMERRGLPAVSLTTDTSVITAVGNDYGFDQ
                     VFSRQVEALGRTGDLLFVFSTSGNSDNLLAAVRQARTQGLKTAALLGKDGGRLKDEVD
                     VFLLAPGGSTDRIQEVHILVGHLVAGLAETELAGR"
BASE COUNT          222 a          423 c          412 g          251 t
ORIGIN      
        1 cctgataacg gccgcgtctt caaaaagata agtgcgttgg ctgcgtcgtc ggcatcgtca
       61 acgtacctcg tccggccggc ttccggcctc ctcgaaccgg acccttttca ggcttccctt
      121 taccttgccg ccggcccccc gtaaatgcta taattatttg ataattcagg aggatagatg
      181 cgcatattca accgattggt agcctggatt tacagcctgg cctcgctcta tgtttcggtc
      241 atcttcatcc tcatctggct cgacccgaac tttaccaatt atttctgcga gcggattcac
      301 gccatcgagc cctacaagtt cgcgctggtg ggcggggccc tgctgatgct gggcgccatc
      361 tggctggcct cggtgatcga gcaccattac cggacgcgct cggtctcctt caacgggccg
      421 ggcggtctgg tccgggtcca gctctcggcg gtcgaggatt tcctgatgaa cgaggtgcgc
      481 cagcatctca aggcggtcaa gcgcatccgc acctccaccc tgatgacgcc caaggggctc
      541 caggtcttca accagctggt catctggtcc ggctacgaca tcccgacggt caccggcaag
      601 gtccaggacc ttatcaagcg cctgctggag gataccatcg gcgtggaacg ggtcggcgat
      661 atccggattt cggtttcccg gatttccgcg ggtgagagcg gcggaccggt cgaagaggac
      721 gaggatgaag aagaactggc cggagaagat acggaaatcc attgaggagg cggtcgccaa
      781 ttaccgccgc ctctccggtc cggaactctc ggagcggctg gcgcagctgg cggagctggt
      841 caccgagacg atcgccggcg gccaccgggt ttacttctgc ggcaacggcg gcagcgccgc
      901 cgacgcccag cacctggccg ccgagttcgt cggccggttc cggatggaac ggcgcggcct
      961 gccggcggtc agcctgacca ccgacacctc ggtcatcacc gcggtcggca acgattacgg
     1021 tttcgaccag gttttcagcc gccaggtcga ggccctgggc cggacgggcg acctgctctt
     1081 cgtgttttcg acttcgggca actcggataa cctgctggcc gcggtgcgcc aggcccgcac
     1141 ccagggcctt aaaaccgccg ccctgctggg caaggacggc ggccgcctca aggacgaggt
     1201 ggacgttttc ctgctcgcgc ccggcgggag caccgaccgg atccaggagg tccatattct
     1261 ggtcggccac ctggtggccg gactggccga gacggagctg gccggccg
//
LOCUS       DAOQYN010000333         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_134893_length_1300_cov_3.460241, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000333 DAOQYN010000000
VERSION     DAOQYN010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134893_length_1300_cov_3.460241"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PKN80_09390"
     CDS             <1..776
                     /locus_tag="PKN80_09390"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNQ36260.1"
                     /translation="AGQPLPRYAGPGDPVGPGYRHAPGGYFNSLIAPVLPYAIRGAIW
                     YQGESNAGFFGAGYEEKFRAMIRSWRRLWAQGDFPFLYVQLARIKKDEGVSRDFREDA
                     SWALLREEQRKSLAEPKTGMAVIFDVTDGDLHPVPKQPIGERLALAALGGVYGRKAVY
                     SGPRVDSVRFKDGQAVISFGETGGGLATLDGKPPRGFVLKAADGKFQAASARIEGKTV
                     VVWSEGVAEPAAVYYGWENMADGNLCNRELLPASPFRAP"
     gene            complement(1063..>1300)
                     /locus_tag="PKN80_09395"
     CDS             complement(1063..>1300)
                     /locus_tag="PKN80_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36261.1"
                     /translation="GFGMGLGLAVRTDPGNAKTHFCLGLLHVLESNPAAALKEHGILK
                     KLDRELARELAGHIQKYRDFTRDLADYIQSQKKP"
BASE COUNT          218 a          390 c          433 g          259 t
ORIGIN      
        1 aggcgggcca gcccctgccc aggtacgccg gtccgggcga tccggtcggg ccgggctacc
       61 gccacgcgcc cggcgggtac ttcaactccc tgatcgcgcc ggtgcttccc tacgcgatac
      121 gcggggccat ctggtaccag ggcgagtcca acgccggctt cttcggtgcc ggctacgagg
      181 agaagttccg ggccatgatc cgctcctggc gccgcctctg ggcgcagggc gattttccct
      241 tcctgtatgt tcagctggcc cggatcaaga aggatgaggg cgtcagccgg gatttccgcg
      301 aggatgccag ctgggcgctg cttcgcgagg agcagcggaa gtccctggcc gagccgaaga
      361 ccggcatggc cgtcatcttc gacgtcaccg acggcgacct ccacccggtg cccaagcagc
      421 cgatcggcga acggctggcc ctggccgccc tgggcggcgt ttacggacgg aaggcggtct
      481 attccggacc ccgggtcgat tcggtgcggt tcaaggacgg ccaggcggtg atatctttcg
      541 gagagaccgg cggcgggctg gccacgctgg acgggaagcc gccgcgggga ttcgtgctca
      601 aggcggccga cggcaaattc caggcggcgt cggcccggat cgaggggaag acggtggtgg
      661 tctggagcga gggcgtggcc gaaccggcgg cggtctatta cggctgggag aatatggccg
      721 acggcaacct ctgcaaccgg gagctgctgc ccgcttcgcc cttccgggcg ccgtgaggga
      781 ggcggctgcg ccggacagcc aggttaagga tttgttctcg gagtgcggct gttttacgga
      841 atatcggtcc gcacggcgcg tcaggggtct tctaaccggg cctctaattc cgatttgaaa
      901 ttctcgtatt ccttctcacg caaccgcttg aaaaaattca taactgcggg acaagggaaa
      961 gagggttttt atccaggccg caaactagag ggctcctggc ccctctgtca atcctccgat
     1021 acattcacgt tttggtcttg catcagccgg gcgggatgga ttttatggtt ttttctggct
     1081 ttggatataa tcggccagat ccctggtgaa atcccggtat ttctgaatat ggccggccag
     1141 ttcccgggcg agttcccggt ccagtttttt taggatgccg tgttctttta aagccgccgc
     1201 cgggttgctc tccaggacgt gcagtaaccc caggcaaaaa tgcgttttgg cattgccggg
     1261 atcggtgcgc acggcaagcc ccagtcccat gccaaaaccg
//
LOCUS       DAOQYN010000334         1295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_135763_length_1295_cov_3.375000, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000334 DAOQYN010000000
VERSION     DAOQYN010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135763_length_1295_cov_3.375000"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1295
                     /locus_tag="PKN80_09400"
     CDS             <1..>1295
                     /locus_tag="PKN80_09400"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HNQ36262.1"
                     /translation="NYELCFAAAGVDARWISTVLDFEGSFFRSRHISAWPEQRPEHET
                     QLKEWVNFLHSLRRPVMSWYPLSLSRPTGRDHPDWRQQFMILDPAKVPYADAVCCINS
                     PYGEAVIAYVIEALKKFDLDGIWFDGAAFSPIWQSPQPVSCVCGHCRRKFRAETGLEL
                     PERYDWSRPEFRRWVAWRYRMFGEYWQRLAERVHREVPEAAIVFNHYHRENIGWNAAV
                     PLNPFGRDFVSGTEADGEPLKGAFHTRLMRAYGRAETEVWMGLACTVRKAVLSGRQPL
                     VNPKDALDFVLACATAGGHPSLGGAEPAHELPVLAAVAAELKPRRPYLDLPSVPYLAL
                     HVSQQTETFVFGRNPDFVTENWVDYYWNSLTGWHHLVAHAGLGCDVLFDDHLGDPRRL
                     EQYPVLVMPLAAALTGDQCRAALDYAAAGGTLVTGPWFGL"
BASE COUNT          187 a          459 c          427 g          222 t
ORIGIN      
        1 aattacgaac tttgtttcgc ggccgccggc gttgacgccc gctggatcag caccgtcctg
       61 gacttcgagg gttccttctt ccgctccagg cacatctcgg cctggccgga gcagaggccg
      121 gagcacgaaa cccagcttaa ggaatgggta aattttcttc attcgctccg gcggccggtc
      181 atgagctggt atcccctgtc gctttcccgg ccgaccggcc gcgaccaccc ggactggcgc
      241 cagcaattca tgatcctgga tccggcgaag gttccctacg ccgacgcggt ctgctgcatc
      301 aattcccctt acggggaggc ggtgatcgct tacgtgatcg aggcgctcaa gaagttcgac
      361 ctggacggca tctggttcga cggggccgcc ttcagcccga tctggcagag tccccagccg
      421 gtctcctgcg tctgcggtca ctgccgccgc aagttccggg ccgagaccgg cctggaactg
      481 ccggagcgct acgactggtc ccggccggag ttccggcgct gggtggcctg gcgctaccgg
      541 atgttcgggg agtactggca gcgcctggcg gagagggtgc accgggaggt gccggaggcg
      601 gccatcgtct tcaaccacta tcaccgggag aacatcggct ggaacgcggc ggtgcccctg
      661 aaccccttcg gccgggattt cgtgagcggc accgaggccg acggcgaacc gctcaagggc
      721 gccttccaca cccggctgat gcgggcttac ggccgggccg agaccgaggt ctggatgggc
      781 ctggcctgca ccgtccgcaa ggcggtcctg agcggccgtc agccgctggt caacccgaag
      841 gacgccctcg atttcgtgct cgcctgcgcc accgccggcg gccatcccag cctgggcggg
      901 gccgagccgg cccatgaact gccggtgctg gccgcggtgg ccgccgaact gaagccccgg
      961 cggccctacc tggacctgcc ctcggtcccc tacctggccc tgcatgtctc ccagcagacc
     1021 gaaaccttcg tcttcggccg caacccggac ttcgtcaccg agaattgggt ggactactac
     1081 tggaactccc tgaccggctg gcaccacctg gtcgcccatg ccggcctcgg ctgcgatgtc
     1141 ctcttcgacg atcacctggg cgatcccagg cggctggagc agtacccggt cctggtgatg
     1201 ccgctggcgg cggccctgac cggggaccag tgccgggcgg cgctcgatta cgcggccgcc
     1261 ggcggcaccc tggtcaccgg cccctggttc ggcct
//
LOCUS       DAOQYN010000335         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_136499_length_1291_cov_3.902104, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000335 DAOQYN010000000
VERSION     DAOQYN010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136499_length_1291_cov_3.902104"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PKN80_09405"
     CDS             complement(<1..1130)
                     /locus_tag="PKN80_09405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNQ36263.1"
                     /translation="MDNQSFKQYRLKKEPTASKIDFKAELNSEQYQVVTGGDGPCLVL
                     AGPGSGKTRTLVYRVAFLLSQSIPPDRILLLTFTNKAAREMLRRVELLLGRYPTGLVG
                     GTFHHAGNLALRRYADRIGYRRNFTILDRSDSKDCLAEVIGELRLGKERYFPKADVLQ
                     GILSFSVNSCRELSAVLDRQYPYLARFQSEIEKAAAAYQARKLAANAMDYDDLLSQWN
                     RLLLFCPEARDYFQEKFRSILVDEYQDTNRLQFEILKSLAGRHRNLMVVGDDAQSIYS
                     FRAAEVRNTLDFKKFFPGARIARLETNYRSVPQILELANRSIAHNQNQFPKELKSTRP
                     AGPRPVLVVTQNVFSEARFVAQRVLELREEGTALSDIAVLFRA"
BASE COUNT          247 a          369 c          425 g          250 t
ORIGIN      
        1 gcccggaaga gcaccgcgat gtcgctgagc gcggtcccct cctcccgcag ctccaggacc
       61 cgctgggcca cgaaacgggc ctcgctgaaa acattctggg tgaccaccag caccgggcgg
      121 ggaccggcgg ggcgggtgct tttaagttcc ttggggaact ggttctggtt gtgggctatc
      181 gagcggttgg ccagttccag gatctgggga acgctccggt aattggtctc cagacgggcg
      241 atgcgggccc ccgggaaaaa cttcttgaaa tcaagggtat tgcgcacctc ggccgcccgg
      301 aaagaataga tcgactgggc gtcgtcgcct acaaccatca ggttgcggtg gcggccggcc
      361 agggacttga gaatctcgaa ctgcagccgg ttggtatcct ggtactcgtc gaccaggatc
      421 gagcggaact tctcctggaa ataatcccgg gcctcagggc agaagagcaa caagcggttc
      481 cactggctga gcaggtcatc gtaatccatg gcgttggccg ccagcttacg ggcctggtaa
      541 gcggcggccg ccttttcgat ctcggattgg aaacgggcca ggtaaggata ctgccggtcc
      601 aggacggccg acagttcccg gcagctgttg acggaaaaac tgaggattcc ctggaggacg
      661 tcggccttgg ggaagtagcg ttccttgccg aggcgcagct cgccgatcac ctcggccaga
      721 caatccttgc tgtcggagcg gtccaggatg gtgaaattgc ggcggtagcc gatccggtcg
      781 gcatagcggc gcagggccag gttgccggca tgatggaagg ttccgcccac cagtccggtc
      841 ggataacggc ccaggagcag ttccacccgc cgcagcattt cgcgggcggc cttgttggtg
      901 aaggtcagca gcaggatccg gtcgggcgga atggactggc tgagcaggaa ggccacccgg
      961 tagaccaggg tccgggtctt gccgctgccg gggccggcca ggaccaggca cggtccgtcg
     1021 ccgccggtaa cgacctggta ctgctcggag ttgagctcgg ccttgaaatc gatcttcgag
     1081 gccgtgggct ccttcttcaa gcggtattgt ttgaaggatt ggttatccat tggttccgga
     1141 ggattcgcct ggcaaggaat accgcgcgtc tctttcattt taccatattc cggtatataa
     1201 tatagttttt aattccaaaa ccccggctac ggcccgcggc cgcggccgcc gccaggtttt
     1261 caaagaccga aaatgggcta cgaaacgatc a
//
LOCUS       DAOQYN010000336         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_138706_length_1279_cov_3.673203, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000336 DAOQYN010000000
VERSION     DAOQYN010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138706_length_1279_cov_3.673203"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PKN80_09410"
     CDS             complement(<1..1038)
                     /locus_tag="PKN80_09410"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNQ36264.1"
                     /translation="MLSDLEIAQQAELKPIREIAAGLGLKETELELYGNHKAKVRLEA
                     LERFQDRPLGKYIDVTAITPTPLGEGKTVTTIGLSQALGRLGKKVITCIRQPSLGPVF
                     GIKGGAAGGGYSQVIPMEDFNLHLTGDVHAVGLAHNLLAAFIDNSLYHGNRLGIEPHS
                     ISWPRVLDVSDRALRNVVIGLGGREDGIPRQSSFDITVASEVMAILALATDLSDLRRR
                     LGRVVVGFDHSGKPVTAEDLKAAGAMAVLLRDAIKPNLLQTLEHTPCLVHAGPFANIA
                     HGNSSVVADRLGIRLAEYLVTESGFGADCGAEKFFNIKCRVSGLKPDAAVVVCSVRAL
                     KMHSGKFRISPG"
     gene            complement(1074..>1279)
                     /locus_tag="PKN80_09415"
     CDS             complement(1074..>1279)
                     /locus_tag="PKN80_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="5,10-methylenetetrahydrofolate reductase"
                     /protein_id="HNQ36265.1"
                     /translation="NENVAGVHVPDTFIKRMADAPKEKRSAESIAIAAELIGAMRGLC
                     RGVHIMPIGWERHLPRVLDTAGL"
BASE COUNT          206 a          391 c          436 g          246 t
ORIGIN      
        1 gcctgggctt atgcgaaact tgccgctgtg catcttgagg gcgcgcaccg agcagacaac
       61 cacggccgcg tccggcttca gcccgcttac ccggcacttg atgttgaaaa acttttccgc
      121 gccgcaatcg gcgccgaatc cgctttcggt gaccaggtat tcggccaggc gtatgccgag
      181 ccggtcggcc accaccgaac tgttgccgtg ggcgatgttg gcgaacggtc cggcgtggac
      241 caggcaggga gtgtgttcca gggtctggag gaggttgggc ttgatggcgt cgcgaagcag
      301 gaccgccatc gctccggccg ccttgagatc ttcggcggtt accggttttc cggaatggtc
      361 gaagccgacc accacccggc ccaaacggcg gcggaggtcg gagaggtcgg tggccagggc
      421 caggatggcc ataacctcgg aggccaccgt gatgtcgaag gaactctggc gcgggatccc
      481 gtcctcccgt ccgcccaggc cgatcacgac gttgcgcagg gcccggtcgc tgacgtcgag
      541 cacccggggc cagctgatcg aatggggttc gattcccagc cggttgccat ggtaaaggga
      601 gttgtcaatg aaggcggcca gcaggttgtg ggccagtccg accgcatgga cgtcgccggt
      661 aagatggagg ttgaaatcct ccatcgggat gacctgggag tagccgccgc cggccgcgcc
      721 gcccttgatg ccgaagaccg gccccaggga gggttggcgg atgcaggtga ttaccttttt
      781 gccgagacgt cccagagcct gggaaaggcc gatggtggtg acggtcttgc cttcacccag
      841 cggagtgggg gtgatggcgg tcacgtcgat gtatttcccg agcggccggt cctggaaccg
      901 ctccagggct tccagccgca ccttggcctt gtggttgccg tagagctcaa gttccgtctc
      961 cttgagcccg agcccggcgg cgatctcccg gatgggttta agttccgcct gctgggctat
     1021 ttccagatca ctcaacatcg ctggcctcct ttaaaaaagg ttccgcccgg ttctcagagc
     1081 ccggcggtgt caagcactct gggcaggtgg cgttcccaac cgatcggcat gatgtggacg
     1141 ccccggcaga ggccgcgcat ggccccgatc agttcggcgg cgatggcgat gctttccgcg
     1201 gaccgcttct ccttgggcgc gtcggccatg cgtttgatga aggtgtccgg cacgtggacc
     1261 ccggcgacgt tttcgttca
//
LOCUS       DAOQYN010000337         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_140023_length_1272_cov_6.060805, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000337 DAOQYN010000000
VERSION     DAOQYN010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140023_length_1272_cov_6.060805"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PKN80_09420"
     CDS             complement(<1..499)
                     /locus_tag="PKN80_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNQ36266.1"
                     /translation="MKNAFGRLWFGRGAGLLAVSGLLLTAAGPAGAQQKPGGPGAPLN
                     PSAETIESVFIKVAEQIKPAVVNISTTQKVTVRNSPFDDFFSDPNFRRFFGDQGPRQR
                     EYSRQSLGSGVIVSADGYILTNNHVVAEAAEIKVKLLDGREFMAKAVGQDAKTDVVVG
                     YVEGVS"
     gene            700..>1272
                     /locus_tag="PKN80_09425"
     CDS             700..>1272
                     /locus_tag="PKN80_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36267.1"
                     /translation="MKRDDFAKIGFYLPEGLPNKEYALSDGLVSVILDQSGGLNMIEY
                     QGPRRTGARRGAFFSNGIWRRRPDFFPEPVIEFRIESAGRSGRPGFANLALCPYGVAG
                     RFEWAGQEFERRLWIKGPAVMMEWRTGGTKPARISLEIREKFLEHLNEHSAWEEPSFD
                     PQWQALRYNILRNFIAQAADRQPTERRHEAC"
BASE COUNT          252 a          422 c          377 g          221 t
ORIGIN      
        1 cggaaacgcc ctccacgtag ccgacaacca catcggtctt ggcatcctgc ccgaccgcct
       61 tggccatgaa ctcgcggccg tccagcagct tcaccttgat ctcggcggcc tcggcgacca
      121 cgtggttgtt ggtcaggatg tacccgtccg ccgaaacgat gaccccgctt ccgagcgact
      181 ggcgggaata ctcgcgctgg cgcgggccct ggtcaccgaa gaaacgccgg aaattcgggt
      241 cgctgaaaaa atcgtcgaag ggactgttcc gtaccgtaac cttctgggtg gtgctgatgt
      301 tgaccaccgc cggcttgatc tgctcggcca ccttgatgaa gaccgactcg atcgtctcgg
      361 ccgaaggatt caacggggcc ccgggtccac ccggcttctg ctgggccccg gccggcccgg
      421 ccgcggtcag cagcaggccc gaaaccgcca gcaatccggc accccttccg aaccacaatc
      481 tcccaaacgc gtttttcacg gagacctcct cgtgatttgg atgaaaaatt tacccccctc
      541 tgttatagaa attagtcgga ccggccgggc aaaaagttta agaacgtgac cggaattcct
      601 aaccggaatc gaacgccggc gccggttccg tccggacatg atacaatgta gaagttccag
      661 gttaaccggc aatgcgctcc ttgattcgcg gagagaaaaa tgaaaaggga tgatttcgca
      721 aagatcgggt tttaccttcc ggaaggattg cccaacaagg aatacgccct ctccgacggg
      781 ctggtgagcg tcatcctgga ccagtccggc ggcctcaaca tgatcgagta ccagggcccg
      841 cgccggaccg gcgcccgccg gggcgccttc ttctccaatg gaatctggcg gcgccggccc
      901 gacttcttcc cggaaccggt catcgagttc cggatcgagt cggccggccg ctccggccgg
      961 ccgggcttcg ccaacctggc cctctgcccc tacggggtgg ccgggcgctt cgaatgggcc
     1021 ggccaggagt tcgaacggcg cctctggatc aaggggccgg ccgtcatgat ggagtggcgc
     1081 accggcggaa cgaagccggc ccggatcagc ctggagatcc gcgaaaagtt cctggagcac
     1141 ctgaacgaac attccgcctg ggaagaaccc tctttcgatc cgcagtggca ggccctccgc
     1201 tacaacattc tcaggaactt catcgcgcag gcggccgacc ggcagcccac cgaaaggcgc
     1261 cacgaggcct gc
//
LOCUS       DAOQYN010000338         1261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_142132_length_1261_cov_3.329187, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000338 DAOQYN010000000
VERSION     DAOQYN010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142132_length_1261_cov_3.329187"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PKN80_09430"
     CDS             complement(<1..452)
                     /locus_tag="PKN80_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNQ36268.1"
                     /translation="MANHEETGLLRRAKRGDRSALEELLRDLEPRLFRVARFLAGNRE
                     EARDLVQETMLALCLRLGAFRGEAKLYTYAYRILVNTFRKRLRKRHLTVPPADYLPYL
                     GRRVASSGAAPDPEAAGVRLRAGLARLPERYRQVIVSHYLEEAPLVEIA"
     gene            574..>1261
                     /locus_tag="PKN80_09435"
     CDS             574..>1261
                     /locus_tag="PKN80_09435"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HNQ36269.1"
                     /translation="MGIKLLSTGSYLPDRVLSNADLEKMVETSDEWITTRTGIRERRI
                     AAPGQAASDLGYESARLALQRADFPAGKLDAIITATITGDHPFPSTGCLIQNRLGAKN
                     AFALDISAACSGFLYGLTLARGLINSGQAGNVLLVASEVMSRVTDYTDRSTCVLLGDG
                     SGAVLLTADKRDAVLGSFIASDGSFAHLLWAPAGGSRQPASHETVNQHLHYMKMEGQA
                     LFKVAVQAMSE"
BASE COUNT          241 a          415 c          389 g          216 t
ORIGIN      
        1 gcgatttcaa ccagcggcgc ctcctccagg taatggctga caatcacctg ccgatagcgc
       61 tccgggaggc gggccagccc ggcccggagc cggacgccgg ccgcctcggg atccggagcg
      121 gctccggagg aagctacccg gcgccccagg taaggcaggt aatcggccgg gggcacggtc
      181 aggtggcgct tccggagacg cttgcgaaag gtgttgacca ggatccggta ggcgtaagtg
      241 tagagtttgg cttcgccccg gaaggctccc aggcgtaggc agagggccag catggtctcc
      301 tggacaaggt cgcgggcctc ctcccggttc ccggccagga agcgggccac tcggaagagg
      361 cgcggctcaa ggtcgcgcaa caactcttcc aaggccgacc tgtcgccccg cttcgcccgg
      421 cgaagcagcc cggtttcttc atggttggcc acgcaaaaac atcatatcaa catcattcgg
      481 gtttgtcaat ccgggggcgc cgggagtata atatccgccg gccttttaaa cttccgccat
      541 tttcccttga ccagacgcaa acggataaaa aacatgggca taaaactcct cagtaccggt
      601 tcctacctgc cggaccgggt tttaagcaat gccgacctgg aaaagatggt cgaaacctcg
      661 gatgaatgga ttaccacccg gaccggcatc cgcgaacgga gaatcgccgc ccccggtcag
      721 gccgcctccg acctcggtta cgaatcggcc cggctcgccc ttcagcgagc cgattttccg
      781 gccgggaagc tggatgcgat catcaccgcc accatcaccg gcgaccatcc ttttccgtcc
      841 accggctgcc tcattcagaa ccggttgggg gccaaaaacg cctttgccct ggacatcagc
      901 gccgcctgct ccgggttcct ctacggactc accctggccc ggggcttgat caactccggc
      961 caggccggca atgtcctgtt ggtggccagc gaggtgatga gccgggtaac cgattacaca
     1021 gatcgttcca cctgcgtgct gctgggcgac gggagcggcg cggtcctgct gaccgcggac
     1081 aagcgggacg cggtgctggg cagttttatc gcctccgacg gcagtttcgc ccacctgctc
     1141 tgggctccgg ccggcggcag ccgccagccg gccagccacg aaacggtcaa ccagcatctt
     1201 cattacatga aaatggaagg acaggccctc ttcaaggtgg cggtccaggc catgagcgag
     1261 g
//
LOCUS       DAOQYN010000339         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_143410_length_1254_cov_4.378649, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000339 DAOQYN010000000
VERSION     DAOQYN010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143410_length_1254_cov_4.378649"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PKN80_09440"
     CDS             <1..1134
                     /locus_tag="PKN80_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNQ36270.1"
                     /translation="VRPSYVLGGRAMEVVYDDEQLARYLTHAVAASEARPVLVDRFLE
                     DATEVDVDAVSDGETTVLGGIMEHIEEAGIHSGDSAMVLPPHSLSKDIIDQIREATHL
                     MARELRVVGLMNVQFAVHEDRLYVLEVNPRASRTIPFVSKAIGVPLAKVATRALMGEK
                     LADLGLTEERWPRYTSVKESVFPFNRFPGVDALLGPEMRSTGEVMGIDRDFALAFAKS
                     QLAAGTNLPTAGAVFLSVKDRDKPEATILGRKLHELGFRLLASHGTAAALAGVGLPVR
                     EVFKVAEGRPDILDFIRNREVAMIINTPSGLRPKRDTVTIRTLAVLNNIPLITTIAGA
                     RAAARAIQQLKTRPFTVHSLQEYHQVWPDPAAGGRPRQNKGVK"
BASE COUNT          218 a          418 c          415 g          203 t
ORIGIN      
        1 gtccgcccct cctacgtcct cggcggccgg gcgatggagg tcgtttacga cgacgagcag
       61 ctggccaggt acctcaccca cgcggtggcc gcctccgagg cgaggccggt cctggtcgac
      121 cggttcctcg aggacgccac cgaggtggat gtcgacgcgg tctccgacgg tgagacaacg
      181 gtcctcggcg ggatcatgga gcatatcgag gaggccggca tccattccgg cgacagcgcc
      241 atggtgctgc cgccccattc gctttccaag gatatcatcg accagattcg ggaggccacc
      301 cacctgatgg ccagggaatt gcgggtggtc gggctgatga acgtccagtt cgcggtacac
      361 gaggaccgtc tttacgttct ggaggtcaac ccgcgggcga gccggaccat tccgttcgtg
      421 agcaaggcca tcggggtgcc gctggccaaa gtggccaccc gggcgctgat gggcgagaag
      481 ctggccgacc tcggcctgac cgaggagcgc tggccgcgtt acacctcggt caaggagtcg
      541 gtcttcccgt tcaaccgttt tccgggcgtc gacgccctgc tggggccgga gatgcgctcc
      601 accggagagg tgatgggcat cgaccgcgat ttcgccctgg cctttgccaa gagccagctg
      661 gccgccggta ccaatttgcc caccgccggg gcggtcttcc tgagcgtcaa ggaccgggac
      721 aagccggagg cgaccatcct gggccgcaag cttcacgaac ttggtttccg gctcctggcc
      781 agccacggca ccgctgccgc cctcgccgga gtcggcctgc cggtccgcga agtcttcaag
      841 gtggccgagg ggcgtcctga catcctcgat ttcatccgga accgtgaggt ggccatgatc
      901 atcaacacgc cttccggcct gaggccgaag cgggacacgg tcaccatccg gaccctggcg
      961 gttttgaaca acatccccct gattaccacc atcgcgggag cgcgggcggc ggcgcgcgcc
     1021 atccagcaac tcaaaacccg ccccttcacg gtgcacagcc tccaggagta ccaccaagtc
     1081 tggccggatc ccgccgccgg cggccggccg cggcaaaata aaggagtaaa gtaatggcaa
     1141 agaatatccg gatcggcgtc tgttcataca gccggaaggg tcagaattcg ggcctgacgg
     1201 ttcccgagca cctggcggcg gccctggatc aggtgcgcca ggcggcggcc gagg
//
LOCUS       DAOQYN010000340         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_144007_length_1251_cov_3.647993, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000340 DAOQYN010000000
VERSION     DAOQYN010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144007_length_1251_cov_3.647993"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PKN80_09445"
     CDS             complement(<1..927)
                     /locus_tag="PKN80_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36271.1"
                     /translation="MAGLKLSEWTLVGPFFAPAGRGNWFPTALDGAAGAPADFRLEGK
                     PVPVRKVRIRGNELDFEAWLGPLADRSRAGIRNQVYAFAEFEVPADLTAELVYDADRY
                     AAWWLDGREVYATRESNGGPVGAMKHRFEIGLSRGRHCLAVRVISSVGRWRVLFRAAR
                     LRPGLADHLRADRGAAWRDYTRSLVRLEERPVPDGTCGPGISKENYELCFAAAGVDGR
                     WISTVLDFEGSFFRSEHISAWPEQKPEHETQLKEWVDFLHSLRRPVMSWYPLSLSRPT
                     GRDHPDWRQQFMLLDPAKVPYADAVCCINSPYG"
     gene            1182..>1251
                     /locus_tag="PKN80_09450"
     CDS             1182..>1251
                     /locus_tag="PKN80_09450"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ36272.1"
                     /translation="MRRKDGFTLIELLVVIAIIAILA"
BASE COUNT          239 a          386 c          427 g          199 t
ORIGIN      
        1 cccgtaaggg gaattgatgc agcagaccgc gtcggcgtag gggaccttcg ccgggtccag
       61 gagcatgaac tgctggcgcc agtccgggtg gtcgcggccg gtcggccggg agagcgagag
      121 gggataccag ctcatgaccg gccgccggag cgaatgaaga aaatccaccc attccttgag
      181 ctgggtttcg tgctccggtt tctgctccgg ccaggccgag atgtgctcgg agcggaagaa
      241 ggagccctcg aaatcaagga cggtgctgat ccagcggcca tccactccgg cggccgcgaa
      301 acaaagctcg taattctcct tgctgatgcc cgggccgcag gtgccgtcgg gaaccggccg
      361 ctcctcgagg cggaccaggc tgcgggtgta atcccgccag gcggcgcccc ggtcggcgcg
      421 caggtggtcg gcgaggcctg gccggagccg ggcggcccgg aagaggactc gccagcggcc
      481 gaccgagctg atcacccgga ccgccaggca gtggcggccc cggctcaggc cgatttcaaa
      541 acggtgcttc atcgcgccga ccgggccgcc gttgctctcg cgggtggcgt agacctcccg
      601 gccgtccagc caccaggccg cgtaacggtc ggcgtcgtag accagttcgg cggtcaggtc
      661 ggccggcacc tcgaactcgg cgaaggcata gacctggttg cggatcccgg cccgggagcg
      721 gtcggccagc ggaccgagcc aggcctcgaa atccagttcg ttcccgcgga tgcgcacctt
      781 ccggaccggg accggcttcc cttccagccg gaaatcggcc ggcgccccgg cggcgccgtc
      841 cagcgcggtc gggaaccagt tgccgcggcc ggccggcgcg aagaagggtc cgaccagggt
      901 ccattcggag agtttcaaac ccgccattta tcctcctggg aaagatgcgg ccggtcaccg
      961 ggtacgattc ccgaaggcct caaccagcgg ttcttttact tgacaaataa aacatcttga
     1021 agtataaaca taaaggaaat cggccgtcaa ggccgggtat ttgtagagag tggccaatgc
     1081 tggacacggt gttaccgttg ttacggaagg aggtgggaac ggccgttaag gtaaaaaagc
     1141 gcgggttcga gatgccagcc atcattatcg agggaggaat catgagaaga aaagacggct
     1201 ttaccctgat cgaactgctg gtggtgatcg ccatcatcgc catcctggcc g
//
LOCUS       DAOQYN010000341         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_145057_length_1246_cov_2.184719, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000341 DAOQYN010000000
VERSION     DAOQYN010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145057_length_1246_cov_2.184719"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKN80_09455"
     CDS             <1..443
                     /locus_tag="PKN80_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNQ36273.1"
                     /translation="ILLFLLGAALVIGGSRLLIFAGTELARSFGISETVIGLTLVAVG
                     TSLPEFFTSVAALTKGHLELSAGNIIGANILDVVWVLGVAGAIRPLPIDSQTSCLALP
                     VLVILSALFVIFGITHRGFKRWEGAVLLLVYALYCLALFRACPV"
     gene            538..792
                     /locus_tag="PKN80_09460"
     CDS             538..792
                     /locus_tag="PKN80_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HNQ36274.1"
                     /translation="MEEKEIGVVADFFGHIGVMALKLTGELKVGDKIHVRGHTTDLTQ
                     VVDSIQVEHEKIDAAAPGMDIGIKVTEKVRHGDRVYRVTE"
     gene            complement(797..>1246)
                     /locus_tag="PKN80_09465"
     CDS             complement(797..>1246)
                     /locus_tag="PKN80_09465"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HNQ36275.1"
                     /translation="ELSRVDPVAAARIQPNDAYRIRRALEVFQAAGRPISRLQSERIP
                     LDLPFIQIGVWRTREEIYRRIEKRVDEIFAAGFLEEVAALRRRYDFSQPAFEAIGYRE
                     AAAVLDGELNLEQARRLIAQKTRNYAKRQLTWFRKDRRILWLDFSGR"
BASE COUNT          229 a          357 c          392 g          268 t
ORIGIN      
        1 agatactcct tttcctgctg ggagcggccc tggttatcgg cggcagccgg ttgctgatct
       61 tcgccggtac cgagctggcc cgcagtttcg ggatttccga gacggtgatc ggcctgaccc
      121 tggtggcggt tggaacctcc ttgccggaat tcttcacctc ggtggccgcc ctgaccaagg
      181 gacacctgga gctgtcggcc ggcaacatca taggggccaa catcctggac gtggtctggg
      241 tactcggcgt ggccggtgcg atccggccgc tgccgattga cagccagacc agctgcctgg
      301 cgctgccggt gctggttatc ctttccgctt tattcgttat tttcggcatc acccaccggg
      361 gcttcaaacg ctgggaaggg gccgtactgc tccttgttta cgcgctttac tgcctggccc
      421 ttttccgggc ctgcccggtt tgaagaaatc gcttccgttt tcctgccttt aaaatcactt
      481 ccgtcccggg tacaataaaa tatacccggc atcagttttt tgaatgggga ggcggatatg
      541 gaggagaagg aaatcggggt ggtcgcggat ttcttcggcc atatcggggt catggccctg
      601 aagttgaccg gggaactcaa ggtcggtgac aagatccacg tcaggggaca caccaccgac
      661 ctgacccagg tggtggattc aatccaggtc gagcatgaaa aaatagacgc ggccgctccg
      721 ggaatggata tcgggataaa agtcacggag aaggtgcgcc acggcgaccg ggtttaccgc
      781 gtgaccgaat aggccgtcag cggccggaaa aatccagcca caggatgcgc cgatcctttc
      841 gaaaccaggt taactggcgc ttggcatagt tgcgggtttt ctgggcgatc agccggcggg
      901 cctgttccag gttgagctcg ccgtccagga cggcggccgc ctcccggtag ccgatggcct
      961 caaaggcggg ctggctgaaa tcgtagcgcc tgcgaagtgc cgccacctct tccagaaatc
     1021 cggccgcaaa aatttcgtcc acgcgtttct ctatccttcg gtaaatttcc tcccgggtcc
     1081 gccagacgcc gatttgaatg aagggtaaat ccaggggtat cctttcggat tgcagccggg
     1141 agatgggccg gccggcggcc tggaagacct cgagggcgcg ccggatacga taggcgtcat
     1201 ttggttggat gcgggcggcg gcgaccgggt ccacccggct taattc
//
LOCUS       DAOQYN010000342         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_145560_length_1243_cov_3.424242, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000342 DAOQYN010000000
VERSION     DAOQYN010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145560_length_1243_cov_3.424242"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..>1243)
                     /locus_tag="PKN80_09470"
     CDS             complement(179..>1243)
                     /locus_tag="PKN80_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNQ36276.1"
                     /translation="ERVLNALARRPVDRVPVFLWFHPETAERLGRRLGIPAGRVGEAM
                     GNDVRQAWVNNNYAMEGIVHERDGESHLDYWGIRWVKEGPFNQIAESPLAAAGPEAIA
                     AYRFPEGRLEELLAPLAATAAGAGDGFLGADVSPCAFEMYLRLRGMEEALLDLAARPE
                     AAAELIGRCAGFAAQLAAAGLDRFRLDWLWTGDDVAGQSAMLMSPAQWRRLIKPALAR
                     VVRVGRERGRPVAYHSCGAIRPIIPDLVEMGVTVLNPIQPNCPGMDPLELKREFGRPL
                     TFMGGVDTQGLLVREPAERIYYHVRELIEGMCSDGGGYILAGSHTIPPETPEANIFAM
                     LEAAGISREEIFDRAAGINP"
BASE COUNT          189 a          476 c          379 g          199 t
ORIGIN      
        1 tggggtagag agagataggt ctttgacttc ttgggtgaag ttcgtctgaa agccaatcga
       61 aaattgcaaa gcttgtcctg agcttgccct gagcccgccc tgagcccgcc ctgagtctgc
      121 ccttagcccg ccctgagctt gtcgaagggc ttgtcgaagg ttcttgctcc gctctgcttc
      181 acggatttat ccccgccgcg cggtcgaaga tctcctcccg gctgatgccg gccgcctcca
      241 gcatcgcgaa gatattcgcc tccggcgtct ccggcggtat ggtatgcgat ccggccagga
      301 tatacccgcc gccgtcgctg cacatcccct cgatcagttc ccgcacgtgg tagtaaatcc
      361 gctccgccgg ctcccggacc agcagcccct gggtatccac cccgcccatg aaggtcagcg
      421 gtcggccgaa ctcccgcttc aactccagcg ggtccatgcc cgggcagttg ggctgaatcg
      481 ggttcagcac cgtcacgccc atctccacca ggtcgggtat gatcggcctg atcgccccgc
      541 aggagtggta agccaccggc cggccgcgct cccgccccac ccggactacc cgggccaggg
      601 ccggcttgat gagccgccgc cattgggccg gggacatcag catcgccgac tggccggcca
      661 cgtcgtcgcc ggtccagagc cagtcgagcc ggaaacggtc caggccggcc gcggccagct
      721 gggcggcgaa gccggcgcag cggccgatca gctccgcggc cgcctccggc ctggcggcca
      781 ggtccagcag ggcctcctcc atgccgcgca ggcgcaggta catctcaaag gcgcagggcg
      841 acacgtcggc gcccaggaag ccgtctccgg ctccggccgc cgtggccgcc agcggcgcca
      901 gtaactcctc cagccgcccc tccgggaacc ggtaggcggc tatcgcctcc ggcccggccg
      961 ccgccagggg tgattcggcg atctggttga agggcccctc cttcacccag cggatgcccc
     1021 agtaatccag gtgcgactcc ccgtcccgct cgtggacgat gccctccatg gcgtaattat
     1081 tattcaccca ggcctgccgg acatcgttgc ccatcgcctc gcccacccgg ccggccggaa
     1141 tccccagccg ccgccccagc cgttcggccg tctccgggtg gaaccagaga aagaccggca
     1201 cccggtcgac cggccgccgc gccagcgcat tcagcacccg ctc
//
LOCUS       DAOQYN010000343         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_145934_length_1241_cov_3.126476, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000343 DAOQYN010000000
VERSION     DAOQYN010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145934_length_1241_cov_3.126476"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /gene="asnB"
                     /locus_tag="PKN80_09475"
     CDS             complement(<1..946)
                     /gene="asnB"
                     /locus_tag="PKN80_09475"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNQ36277.1"
                     /translation="MCGIVGRVEKSGRPADPGLLARMTRRLAHRGPDAEGFHLDGPVG
                     LGHRRLSVIDLAGGGQPMANETGRLQLVVNGEIYNFQALRRELAGRGHRFRSQTDSEV
                     ILHLYEEAGPDCLRRLRGMFAFALWDQDRRRLFLARDRIGKKPLVYAETPESFSFASE
                     LQALLEAPDLKKEIDPAALDDFLTLQYIPSPRSIFAGIRKLPPAHYLLYEEGRTRVEP
                     YWSPDFNRKLKVDADEAGQLLLNKLAEAVQIRLVSDVPLGAFLSGGLDSSAVVGLMSL
                     ASPRPVRTFSIGFSEESYSELKYAALAARAFRTEHREFM"
     gene            complement(946..>1241)
                     /gene="ribH"
                     /locus_tag="PKN80_09480"
     CDS             complement(946..>1241)
                     /gene="ribH"
                     /locus_tag="PKN80_09480"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNQ36278.1"
                     /translation="FEIPAVAKKLAGGGRFDAVICLGAVVRGETPHFDYIAGQSSKAI
                     GQLSLEMKVPVIYGILTCDTVEQAMDRAGAKSGNRGWQAALTALEMASLYQVI"
BASE COUNT          194 a          415 c          435 g          197 t
ORIGIN      
        1 ccatgaactc ccgatgctcg gtccggaagg cccgggccgc cagcgcggcg tacttcaatt
       61 cgctgtacga ttcctcggag aagccgatcg aaaaggtccg caccggccgg ggcgaggcca
      121 ggctcatcag gccgaccacg gcgctcgagt cgagtccgcc ggagaggaag gcgcccaggg
      181 ggacgtcgct caccagccgg atctggaccg cctcggccag cttgttcagc aggagctggc
      241 cggcctcgtc ggcgtcgact ttcagcttgc ggttgaagtc cgggctccag taaggttcga
      301 cccgggtccg gccctcctcg tagagcaggt agtgggccgg cggtagcttg cggatgccgg
      361 caaagatgct gcgagggctg ggaatgtact gcagggtgag aaaatcgtcc agggcggccg
      421 ggtcgatctc cttcttcagg tcgggcgcct ccagcagggc ctgcagttcc gaggcgaagc
      481 tgaacgactc cggggtttcg gcatagacca gcggcttctt gccgatccgg tcccgggcca
      541 ggaagaggcg ccgccggtcc tggtcccaga gggcgaaggc gaacatgccc cgcagccgcc
      601 gcaggcaatc cggccccgct tcctcgtaaa ggtggaggat cacctcactg tcggtctggg
      661 agcggaaccg gtggccgcgt ccggcgagtt cccggcgcag cgcctggaag ttgtagatct
      721 cgccgttgac gaccagctgg agacggccgg tttcgttggc catcggctgg ccgccgccgg
      781 ccaggtcgat caccgagagg cgtcggtggc ccagccccac cggcccatcc aggtgaaacc
      841 cctcggcgtc cggaccgcgg tgggcgagcc ggcgggtcat tcgcgccagc agcccggggt
      901 cggccggccg gcccgatttt tcaactcggc cgacgatgcc gcacatcaga tgacttggta
      961 aagactggcc atctccaggg cggtcagggc cgcctgccag ccgcggtttc cggacttggc
     1021 gccggcgcgg tccatggcct gttcgaccgt gtcgcaggtg aggatgccgt agatgaccgg
     1081 caccttcatc tccagcgaga gctggccgat ggccttgctc gactggccgg cgatatagtc
     1141 gaagtgcggc gtctcgcccc ggaccaccgc ccccaggcag atgaccgcgt cgaagcggcc
     1201 gcccccggcc agcttcttgg ccacggccgg gatttcgaag c
//
LOCUS       DAOQYN010000344         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_146055_length_1241_cov_1.998314, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000344 DAOQYN010000000
VERSION     DAOQYN010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146055_length_1241_cov_1.998314"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(724..>1241)
                     /locus_tag="PKN80_09485"
     CDS             complement(724..>1241)
                     /locus_tag="PKN80_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNQ36279.1"
                     /translation="RFFLLKHLRRLGYQVGIGGIVGLPGQSLKDLAEDLRTVGDFQPE
                     MVSFGPYLTHHQTPLAEAPNGSEALTLKMMALARLLAGRANMPVTTALATLDPSDGLT
                     RGLRAGANVIMVNFTPEPRRFNYTIYDHKLRVDFEQVRIAAARAGRTVSMERGDAPIK
                     EGKEKERQGED"
BASE COUNT          269 a          361 c          363 g          248 t
ORIGIN      
        1 ctcctgggcc gcctggagaa tcggtatcag ggcgcccggt ttgcgacggt aaagttcgac
       61 ggcattccgc aactgctcca atccggccgc ctcgtcaagc gcaacctcgc aacaaccgga
      121 tttcttcttg tcggtcattc cgttcctccg gccttgactc cgggatggcg gccgttcgtg
      181 cctttgagta cgggaagcga aaggccggcc ccggggctga aaaaccttta ttatggagat
      241 ttacgaataa agcggtccct gcggagaggt taaaactggt taaagaggcc gttttaatcg
      301 ttattaaaag cacaaacctt gatttgtcca attttatccc acgccggcct gcttgtcaat
      361 ctcccgaaaa tggtctccgg ggctgtttta aggacggatc cggcccggaa cccggtaaat
      421 ggccgccttg gtaatgatca gaccggcgga caagtccgta acgcttcccc ggatagacga
      481 ccaacatcca tcgtaccact cgccgggaga tgcgaacaga aacggccgga cgcctgaatt
      541 cctttccatt caataagatt gactgcctcc aaaacgacag gcctgacaaa acattcaaag
      601 gctggattcc cggggggaac ggaagacaca caacctagag actcgactaa atcattcgat
      661 gggaggccag gcggacctga ccgaaaaaaa agcaggcggg aatggcgttc aggaaagagg
      721 tgtttaatcc tctccttgtc tttctttttc ctttccttcc tttatcgggg cgtcgccgcg
      781 ctccatcgaa acggttcgcc cggcccgggc ggcggcaatc cgaacctgtt cgaaatcgac
      841 ccggagcttg tggtcgtaaa tggtgtagtt aaaacggcgc ggttccgggg tgaagttgac
      901 catgatcacg ttggcgcccg cccgcagccc ccgggtcagg ccgtcggacg gatccagggt
      961 ggccagggcg gtggtaaccg gcatattggc ccgaccggcc agcagccggg ccagcgccat
     1021 catcttcagg gtcagggctt ccgaaccgtt cggcgcttcc gccagcggcg tctggtggtg
     1081 agtcaggtag ggtccgaacg agaccatctc gggctgaaaa tcgccaacgg tccgcagatc
     1141 ctcggccagg tctttcaggg actgccccgg caggccgacg ataccgccga tgccgacctg
     1201 gtagccgaga cggcgcaggt gcttgagcag gaaaaaccgg t
//
LOCUS       DAOQYN010000345         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_151369_length_1215_cov_2.975000, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000345 DAOQYN010000000
VERSION     DAOQYN010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151369_length_1215_cov_2.975000"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            466..1122
                     /locus_tag="PKN80_09490"
     CDS             466..1122
                     /locus_tag="PKN80_09490"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ36280.1"
                     /translation="MKVLLISASPHRLKSNTFILADEARRACAAAGAETETVHLPDLE
                     IKFCLHCEACHREILACPLKDDVRPLLDKMLEADGLILATPNYINQVTASMKALFDRS
                     SHFIHCLRLLGKHVAGVVSSGSGRDVEVLDYLAHYAHTCGAWYSGGLSSSVAAVPENR
                     DRARELGVKLAADIKEKRTFPDQARVLQEHRVHFKRLIEARKDDWPGEYQYWQKQGWL
                     "
BASE COUNT          221 a          400 c          336 g          258 t
ORIGIN      
        1 gaaagaccgg cacccggtcg accggccgcc gcgccagcgc attcagcacc cgctctcttg
       61 aagtcatgcc cgtcccctcc ttatgccttt ccagtccgct ttttcagttc aaaacctttt
      121 aaggattgga tacccgcctg aagcgtgcgg gtatgacatg ctttttaaag attcgtctga
      181 aaaaccaatc caaagtcctg aagtgtatgt cgaaggctct tcaaagaaga gggctgagct
      241 tgtcgaaggc cttcagggga ttataattat aatcataatc ggcttgcgcc tccgcgccgg
      301 cggccggagg gtcccggtct caggcttatt atagcgcgcc cgccccttcc cggccagggg
      361 ttgcgccggc cggcggccgg gtggtaatat cttgacggcc tctttttatc cttttctctt
      421 caccctgttt tttcgaggcg cacaccgggc aagaaaggag cacccatgaa agtcctgctc
      481 atctccgcca gtccgcaccg gctcaagagc aacaccttta tcctggccga cgaggcccgg
      541 cgcgcctgcg cggcggccgg cgccgagacc gagaccgtcc acctgcccga cctggaaatc
      601 aagttctgcc tccactgcga ggcctgccac cgggagatcc tggcctgtcc cctgaaggac
      661 gatgtccgcc ccctcctgga taagatgctc gaggccgacg gcctcatcct ggccaccccc
      721 aactacatca accaggttac cgcctcgatg aaggccctct tcgaccgttc cagccacttc
      781 atccactgcc tgcgacttct cggcaagcat gtcgccggcg tggtctcctc cggcagcggc
      841 cgggatgtcg aggtgcttga ttacctggcc cactacgccc atacctgcgg cgcctggtac
      901 tccgggggcc tctcctcgtc cgtggcggcc gtcccggaaa accgggaccg ggcccgggag
      961 ttgggcgtca agctggccgc cgacatcaag gaaaagcgga cctttccaga ccaggccagg
     1021 gtactgcagg agcaccgcgt ccatttcaag cgcctgatcg aggcccggaa ggacgactgg
     1081 ccgggtgagt accagtactg gcaaaagcag ggctggttgt aaaaggtggg tattttttcg
     1141 cccgacatgc ctttgcacct ttctttagcc cgtcattcct gcgggtcttt tcttgagttt
     1201 gtcattcccg cggat
//
LOCUS       DAOQYN010000346         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_153242_length_1206_cov_3.980886, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000346 DAOQYN010000000
VERSION     DAOQYN010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153242_length_1206_cov_3.980886"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKN80_09495"
     CDS             <1..391
                     /locus_tag="PKN80_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNQ36281.1"
                     /translation="LRSSDRPGRVILAFGCGGDRDRSKRPKMGEVAARLADLTILTSD
                     NPRSEEPEAIIRDIEAGIPAGRRDRVEVVADRQSAIQRALELARPGDLVLIAGKGHET
                     YQILKDTVIPFDDRLAVRKALAGLKGV"
     gene            398..>1206
                     /gene="murF"
                     /locus_tag="PKN80_09500"
     CDS             398..>1206
                     /gene="murF"
                     /locus_tag="PKN80_09500"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNQ36282.1"
                     /translation="MEPVSLKEILAWTGGRRLQGGLKKPVRRFSTDTRKIEPGDFFIP
                     VAGASFDGHDFIPAAAAAGALGSLCGAGRRPAAKLPENFLLVEAPGDTRAALLDLARH
                     YRLRFQIPLLALTGSSGKTTTKELLTHLLAGRFRVLANRGNFNNEIGLPLSIFELERR
                     HRAAVLELGMNHPGEIAALARTCRPGFGLITNIGTAHLGLMGSRRAIAAAKAELLAEL
                     AGGTALLPADDAFYPFLAGCPVRRRVSFGLKATADYRVVRPQPGAGATAFLL"
BASE COUNT          180 a          495 c          372 g          159 t
ORIGIN      
        1 cctgcgcagc tccgaccggc ccggccgcgt catcctggcc ttcggctgcg gcggcgaccg
       61 cgaccggagc aagcggccca agatgggcga agtggccgcc cgcctcgccg acctgaccat
      121 cctcacctcc gacaacccgc gcagcgagga gcccgaggcc atcatccgcg acatcgaggc
      181 cggcatcccg gccggccgcc gcgaccgcgt cgaggtggtg gccgaccggc agtcggccat
      241 ccagcgcgcc ctggaactgg cccggcccgg cgacctggtc ctcatcgccg gcaagggaca
      301 cgagacctac cagatactca aggacaccgt catccccttc gacgaccgcc tggccgtccg
      361 caaggcgctg gccggactca agggcgtctg aagcccgatg gaacccgtct ccctgaaaga
      421 gatcctggcc tggaccggcg gccgccgact ccagggcgga ctcaaaaaac cggtccgccg
      481 cttcagcacc gacacccgga agattgaacc gggcgacttc ttcatcccgg tcgccggcgc
      541 cagtttcgac ggccacgact tcatcccggc cgccgccgcc gccggcgcgc tcggttccct
      601 ctgcggggcc ggccggcggc ccgcggcgaa gcttccggaa aacttcctgc tggtcgaggc
      661 gccgggcgac acccgggccg ccctgctgga cctggcccgc cactaccggc tccggtttca
      721 gataccgctc ctggccctga ccggcagcag cggcaagacc accaccaagg aactgctcac
      781 tcacctgctg gccggccgct tccgggtcct ggccaaccgc ggcaacttca acaacgaaat
      841 cggcctgccc ctctcgattt tcgaactgga gcgccggcac cgggcggccg tcctcgaact
      901 gggcatgaac caccccggcg agatcgcggc cctggcccgg acctgccgcc ccggcttcgg
      961 cctcatcacc aacatcggta ccgcccacct gggcctgatg ggcagccgcc gggccatcgc
     1021 cgccgccaag gccgaactgc tcgccgaact ggccggcggc accgccctgc tgccggccga
     1081 cgacgccttt tatcccttcc tggccggctg cccggtgcgc cgccgcgtca gtttcggcct
     1141 caaggcgacc gccgattacc gggtggtgcg tccgcagccc ggcgccggcg ccaccgcctt
     1201 cctcct
//
LOCUS       DAOQYN010000347         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_164420_length_1157_cov_2.596189, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000347 DAOQYN010000000
VERSION     DAOQYN010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164420_length_1157_cov_2.596189"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..>1157)
                     /locus_tag="PKN80_09505"
     CDS             complement(193..>1157)
                     /locus_tag="PKN80_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36283.1"
                     /translation="GKPAVFLGKFECRLQEENVLLLNDFSLELDGRTVYQGPVSGAWH
                     THFYPAPEGTPFRAVYEFESAVELEGCFAAIEMAENLDRIEFNGVPVKPRKSRGDNGP
                     FSPGRSWKDINFTRVDLPALRKGLNQLVIAGLKVNNISRSGCHHRVEAWRKHFPTEAE
                     EIYLCGHFSLEKLAEGRYRVFRYRTPAGRNLAVEGYPFYSGRLTYRADFCWRGRYGGG
                     AWLKLNGVSWLASIRVRVNGRDCATRRWAPWLVDISAALQPGENHLELDTAVTLVNAF
                     GPNRQAGIKQDTGIGPRTFVLGDRFTRDYQLFEFGLENAVIYRR"
BASE COUNT          223 a          365 c          362 g          207 t
ORIGIN      
        1 ctcgctgcag aaagggtaac ccagcaccgc gcagaaatcg atgttctccc ggccggaatc
       61 aaggtagtgg tgatcatggt ccgggtcctc gaagtgggtg tgcaggtccc cccagagcag
      121 ccgatactca tccatgccct tctccggtcg gttgaaagcg ccccggttca agccggaagc
      181 ggtcggagcc gcctatctgc ggtagatgac cgcgttctcc agcccgaatt cgaagagctg
      241 gtagtcgcgg gtgaagcgat cgcccaggac gaaggtgcgc ggtccgatgc cggtatcctg
      301 tttgattccg gcctggcgat tgggtccgaa agcattgacc agcgtcaccg ccgtgtccag
      361 ttccaggtgg ttctcgcccg gctgcagggc cgcagaaatg tccaccagcc agggcgccca
      421 gcgccgggtg gcgcaatcgc ggccgttgac ccggacccgg atcgaggcca gccaggagac
      481 cccgttgagt ttaagccagg cgccgccgcc gtatcgtccc cgccagcaga aatcggcccg
      541 gtaggtcagg cggccggagt aaaaaggata tccttccacc gccaggttcc ggccagccgg
      601 ggttcgataa cggaagaccc ggtagcggcc ctcggccagt ttctcgaggg aaaaatggcc
      661 gcagaggtag atctcctcgg cctcggtcgg aaaatgcttc cgccaagcct cgacccggtg
      721 gtggcagccg gaccggctga tgttgttcac cttgaggccg gcaatgacca gctggttcaa
      781 tcccttccgc aaggccggca ggtcaacccg ggtaaagttg atatccttcc aggagcgtcc
      841 tggtgaaaaa ggtccattgt caccccgcga tttccggggt ttgaccggta cgccgttaaa
      901 ctcgatccgg tcgaggttct cggccatctc gatggccgcg aagcagcctt ccagttccac
      961 cgccgattca aattcgtaaa ccgcccggaa gggtgtaccc tccggggccg ggtagaagtg
     1021 ggtatgccag gccccggaaa ccggcccctg gtagaccgtc cggccgtcca actccaggga
     1081 aaaatcgttc aggagcagga cattctcctc ctggagacgg cactcgaatt tccccaggaa
     1141 gacggccggt ttcccgg
//
LOCUS       DAOQYN010000348         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_166077_length_1150_cov_4.188128, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000348 DAOQYN010000000
VERSION     DAOQYN010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166077_length_1150_cov_4.188128"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PKN80_09510"
     CDS             complement(<1..465)
                     /locus_tag="PKN80_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNQ36284.1"
                     /translation="MLNELELVMRLVLAAALGGIIGIERESHGREAGFRTMILVCVGN
                     CLVLVISLQMITLGGAAGTFASVPALRLDPSRIAAGALTGIGFLGAGAIIQDRRRIRG
                     LTTAACLWVVTAIGLAVGGGFYAVGLAAAAITLIALYFLRPLEHLFKRDNYNI"
     gene            607..>1150
                     /locus_tag="PKN80_09515"
     CDS             607..>1150
                     /locus_tag="PKN80_09515"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNQ36285.1"
                     /translation="MRAESILIPLTAALLLAGTAGLEAAPRAGETLRYQLKWNVVSGG
                     FMNVSVEEAASVDSESTYHLKAYMETGGLFKKLYPFTANIDSYPAVSDFLPRRYLYRS
                     NSKEERIAETVDFSRTPPEGTWKLDKFEYDRNRSQKKSDRIKTSGFFQDPLSIIYYLR
                     GQKLQVGSTLTVPLYMDRREY"
BASE COUNT          225 a          388 c          365 g          172 t
ORIGIN      
        1 gatgttgtaa ttgtcgcgct tgaagaggtg ttcgagcggc cgcagaaagt agagggcgat
       61 cagggtgatc gcggccgcgg ccaggcccac cgcgtagaag ccgccgccca cggccagtcc
      121 gatggcggtc accacccaga ggcaggccgc ggtggtcaag ccccggatgc ggcggcggtc
      181 ctggatgatg gccccggcgc ccaggaagcc gatgccggtg agggcgccgg cggcgatgcg
      241 cgaggggtca agacgcaggg ccggcaccga ggcgaaggtg ccggcggccc cgcccagggt
      301 tatcatctgg agcgaaataa ccagcaccag gcagttgccg acgcagacca ggatcatggt
      361 ccggaagccg gcctcccggc cgtggctctc ccgttcgata ccgatgatgc cgcccagggc
      421 ggccgccagc accaggcgca tcaccagttc cagctcgttt aacattaagg cctcccggat
      481 cgcaggcgcg gcgccggcca gggagcgccc cgcgctgcca ggcggaatgg gaccttcctc
      541 aaaccctggc catggggtag tttagcactt ttacagcggc cgggcaaata ggttaaaatt
      601 agtttcatgc gcgccgaatc aatcttgatc ccgctgaccg cagcgctcct gctggccggg
      661 acggccggcc tggaggccgc gcccagggcc ggggagacgt tgcgctacca gctcaagtgg
      721 aacgtggtct ccggcggctt catgaacgtt tcggtcgagg aggcggcctc ggtggacagc
      781 gagtccactt accacctcaa ggcctacatg gagaccggcg gcctcttcaa gaaactctac
      841 ccgttcaccg ccaacatcga ctcctacccg gcggtcagcg atttcctgcc ccgccgctac
      901 ctttaccgct ccaacagcaa agaggaacga atcgccgaga cggtcgactt cagccggacg
      961 ccgcccgagg gaacctggaa actggacaag ttcgagtacg accgcaaccg cagccagaag
     1021 aagtcggacc ggatcaaaac ctccggcttc ttccaggacc cgctctccat catctactac
     1081 ctgcgcggcc agaagctgca ggtcggctcc accctcacgg taccgctcta catggaccgc
     1141 cgcgagtacc
//
LOCUS       DAOQYN010000349         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_166085_length_1150_cov_3.540639, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000349 DAOQYN010000000
VERSION     DAOQYN010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166085_length_1150_cov_3.540639"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1150
                     /locus_tag="PKN80_09520"
     CDS             <1..>1150
                     /locus_tag="PKN80_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNQ36286.1"
                     /translation="RREESGTAYPADTEWQSEFENEFLYEETPDQQTAIAETKANLAS
                     PRPMDRLICGDVGYGKTELAMRAAFKVVESGRQVALICPTTILAEQHRRTFRERFADF
                     PVRIENLSRFRSRKEQQAILADLADGKVDIIIGTHRLLSRDVRFKKLGLWVVDEEQRF
                     GVTHKERLRANFPNLDILTLTATPIPRTLQLSLVGIRDLSVIETPPAGRLSILTFVSP
                     YRTKTVVRACRRELERDGQVFFIHNRVEKIERVADHLKGLVPEARIDIAHGQMEERQL
                     SGVMERFLDRQTDILVSTSIIESGLDIPTANTIIINNGQNFGLADLYQLRGRVGRYST
                     QAYCYLCYDPGAVLTDEARRRLKAMEDFSRLGSGMRLALADLEIRGAG"
BASE COUNT          222 a          404 c          352 g          172 t
ORIGIN      
        1 agcggcgcga ggaatccggc accgcctacc cggccgatac cgaatggcag tcggaattcg
       61 aaaacgaatt tctttacgag gagacgcccg accagcagac ggccatcgcg gaaaccaagg
      121 ccaacctcgc ctcgccccgg cccatggacc ggctcatctg cggcgatgtc ggctacggca
      181 agaccgagct ggcgatgcgg gcggccttca aggtggtgga gagcgggcgg caggtggccc
      241 tgatctgccc gaccaccatc ctggccgaac aacaccggcg caccttccgg gaacgcttcg
      301 ccgacttccc ggtccgaatc gagaacctct cgcgtttccg cagccggaag gagcagcagg
      361 ccatcctggc cgacctggcc gatgggaagg tggacataat catcggcacc caccggctgc
      421 tcagccggga cgtccgcttc aaaaaactcg gcctctgggt cgtggacgag gagcagcgct
      481 tcggcgtcac ccacaaggag cggctccggg ccaactttcc caacctcgac attctcaccc
      541 tgaccgccac acccatcccg cgcaccctgc agctttcgct ggtcggcatc cgagacctct
      601 cggtaatcga aaccccgccg gccgggcgcc tctccatcct gaccttcgtc tcgccctacc
      661 ggaccaaaac ggtcgtccgg gcctgccgcc gcgaactgga acgtgacggc caggtcttct
      721 tcatccacaa ccgggtggag aagatcgaac gggtggccga ccatctgaag ggcctggtgc
      781 cggaggcccg aatcgatatc gcccacggcc agatggaaga gcggcagctc tccggggtga
      841 tggaaaggtt cctggaccgg cagaccgaca tcctggtctc cacttcgatc atcgaatccg
      901 ggctggacat cccgaccgcc aacacgatca tcatcaacaa cgggcagaac ttcggcctgg
      961 ccgacctcta ccagctgcgc ggccgggtcg gccgctacag cacccaggcg tactgctatc
     1021 tctgctacga cccgggggcg gtcctgaccg atgaggcccg ccgccgtctg aaggcgatgg
     1081 aggatttcag ccggctgggc agcgggatgc ggctggccct ggccgacctg gagatacgcg
     1141 gcgccggcaa
//
LOCUS       DAOQYN010000350         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_169896_length_1135_cov_3.983333, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000350 DAOQYN010000000
VERSION     DAOQYN010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169896_length_1135_cov_3.983333"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1135)
                     /locus_tag="PKN80_09525"
     CDS             complement(<1..>1135)
                     /locus_tag="PKN80_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNQ36287.1"
                     /translation="QPVPPVRRALQARQVRPDKDFTLFYDTDAGPFGVSLAAYRPERE
                     AGFFMLTAAPAVEAGAAAVLPKDVVFVLDTSGSMAGKKLDQARRALNYCLERLNREDR
                     FELVRFATEAEPLFSRLAPAGPKERDRARESVAGLKARGGTNIEEALARALRVFDGSR
                     ERPQMVVFITDGQPTVGALDEDTILRTVKPAAAAVRVFTFGVGDDLNTRLLDRITEAT
                     RASRTYVGTNEDLEVKLTGFFDRVEAPVLVNPRIEFRGVRVSELCPRELPDIFRGSQL
                     LVFGRYQEAGEATLILEGTVEGRRRSYEYRVNFPERSGEHDFIAPLWAAQRIGQLLDQ
                     IRLAGESSELKDEIVELARTYGIVTPYTSYLVVEEEAKRLADNR"
BASE COUNT          167 a          407 c          393 g          168 t
ORIGIN      
        1 tccggttgtc ggccaggcgt ttcgcctcct cctcgaccac caggtagctg gtgtaaggag
       61 tgacgatgcc ataagtccgg gccagctcga cgatctcatc cttgagctcc gacgactccc
      121 cggccagtcg gatctggtcg agcagctggc cgatccgctg ggccgcccag agcggcgcga
      181 tgaagtcatg ctcgccggac cgctccggaa aattgacgcg gtactcgtaa ctccggcgcc
      241 gcccctcgac cgttccctcc aggatcagcg tcgcctcacc cgcctcctgg tagcggccga
      301 agaccagcag ctggctgccc cggaagatgt cgggcagttc gcgcgggcag agctcggaga
      361 ccctgacgcc gcggaactcg atccgggggt tgaccagcac cggcgcctcg accctgtcga
      421 agaagccggt cagcttgacc tccaggtcct cgttggtgcc gacataggtc cggctggccc
      481 gggtggcctc ggtgatccgg tccagcaggc gggtgttcag gtcgtcgccg accccgaagg
      541 tgaagacccg gaccgcggcc gccgcgggct tgaccgtccg caaaatggta tcctcgtcca
      601 gggcgcccac ggtcggctgg ccatcggtga tgaagacgac catctgcggc cgctcccggc
      661 tgccgtcgaa gacccggagc gcccgggcca gggcctcctc gatattcgtc ccgccgcgcg
      721 ccttcaagcc cgccaccgac tcccgggccc ggtcgcgctc cttcggcccg gccggggcca
      781 ggcggctgaa gagcggctcg gcctcggtgg cgaagcggac gagctcgaag cggtcctcgc
      841 ggttcaggcg ttccaggcag taattgagcg cccggcgcgc ctggtccagc ttcttgccgg
      901 ccatgctgcc ggaggtgtcg agcacgaaga ccacgtcctt gggcaggacc gcggccgccc
      961 cggcctccac cgccggcgcg gccgtgagca tgaagaagcc ggcctcccgc tccggccggt
     1021 aggcggccag ggagacgccg aaggggccgg catcggtgtc gtaaaagagg gtgaagtcct
     1081 tgtccggccg gacctgccgg gcctgaaggg cccgccggac cggcgggacc ggctg
//
LOCUS       DAOQYN010000351         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_170682_length_1132_cov_3.433612, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000351 DAOQYN010000000
VERSION     DAOQYN010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170682_length_1132_cov_3.433612"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..>1132)
                     /locus_tag="PKN80_09530"
     CDS             complement(279..>1132)
                     /locus_tag="PKN80_09530"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNQ36288.1"
                     /translation="HGFRPLCLGRLHDGWVVASETSALDILEAEYQRDVEPGEIVILG
                     SDGLESLRPFPARKRAFCIFEYIYFARPDSRVFGHGVYQVRKRLGERLAVEAPAQGDF
                     AMPVPDSGTCAALGFSQKAGLPFEMAFVRNHYVGRTFIQPVQRVRDLNVKIKLNPVRE
                     LIEKRRLVLIEDSIVRGTTSRGRVRTLRNFGAREIHMRISCPPHRFPCYYGIDFPDPA
                     ELAANRLSIEELRRYLGLDSLNYLSLEGMLAAMPLPAGDFCTACFSGKYPVAPEAGLN
                     KEVLEKK"
BASE COUNT          198 a          352 c          401 g          181 t
ORIGIN      
        1 atgaggaggc ctgggaagag ggcaatgcct gagcgggctt gaccggctct ccctggatta
       61 aacgcggaac ggcagtttca gtaaggaagt gacggccggc ctggccacct tcatgaccag
      121 gccggcctcc tctttgtcca ccccggccgg ccaagggcgg ctgatcccgg accgccaagg
      181 aataacctgc caggctcttg ccaggggcct gaattaaacc gttatctctt caaagccggg
      241 tgcggctggg tgggaggcca aataaagcgg gcggggtctt atttcttttc caggacttcc
      301 ttgttcaggc cggcctccgg cgccaccggg tatttcccgg agaagcaggc ggtgcagaag
      361 tcgccggccg gcagcggcat ggccgccagc attccctcca gcgaaaggta attcaaactg
      421 tccaacccca ggtagcggcg cagctcctcg atcgaaaggc ggttggccgc cagttcggcc
      481 gggtcgggga aatcgatgcc gtagtagcag gggaagcggt gcggcgggca gctgatgcgc
      541 atgtggatct cccgggcccc gaagttgcgc agggtccgga cccggccccg gctggtggtg
      601 ccgcggacga tcgagtcctc gatcaggacc agccgccgct tctcgatcag ttcgcggacc
      661 gggttgagct tgatcttgac gttcaggtcg cgcacccgct ggaccggctg gatgaaggtc
      721 cggccgacgt agtggttgcg gacgaaggcc atctcgaagg gcaggccggc cttctggctg
      781 aagcctaggg cggcgcaggt gccggagtcg ggcaccggca tcgcgaaatc gccctgcgcc
      841 ggcgcctcga cggccagccg ttcgcccagg cgcttgcgca cctggtagac gccgtggccg
      901 aagacccggc tgtcgggccg ggcgaagtag atgtactcga agatgcagaa ggcgcgcttg
      961 cgggccggga acggccggag cgattcgagc ccgtccgaac cgaggatgac aatttcgccc
     1021 ggctcgacat cgcgctggta ctcggcctcc aggatgtcga gggccgaggt ctccgaggcc
     1081 accacccagc cgtcatggag ccggcccagg cagagggggc ggaagccatg cg
//
LOCUS       DAOQYN010000352         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_170922_length_1131_cov_3.153346, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000352 DAOQYN010000000
VERSION     DAOQYN010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170922_length_1131_cov_3.153346"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PKN80_09535"
     CDS             <1..735
                     /locus_tag="PKN80_09535"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNQ36289.1"
                     /translation="YKEAMQRGYIGDLMEAGAHLLDLNAGLGSGDEAAGLRWLVETVQ
                     ALGYRDICLDSADPAALQACLPLVQGRVLVNSINGEAAKLEALLPVLDGYPGEVVGLT
                     MDDRGIPGDVKTRLEITARMVKAMEGCRVPVERIYIDPLTQPVSTDPKNGRVFLESLR
                     AIKREFPGIKTTCGLSNVSFGLPERRVLNRCFLALAIEAGLDSALIDPTDQGMMAAML
                     TAEGLTGIDQYCMSYLTAFREGKIKS"
     gene            1054..1130
                     /locus_tag="PKN80_09540"
     tRNA            1054..1130
                     /locus_tag="PKN80_09540"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1088..1090,aa:Pro,seq:ggg)
BASE COUNT          203 a          330 c          384 g          214 t
ORIGIN      
        1 tacaaggagg cgatgcagcg cggctacatc ggcgatctga tggaggccgg cgcccacctg
       61 ctggacctta acgccgggct cggttccggc gacgaggccg ccggtttgcg ctggctggtg
      121 gagacggtcc aggccctcgg ttaccgggac atctgcctgg acagcgccga tccggccgcc
      181 ctccaggcct gcctgcccct ggtccaggga cgggtgctgg tcaattccat caacggcgag
      241 gcggccaagc tggaggccct gctgccggtg ctcgatggtt atcctggcga ggtggtcggg
      301 ctgaccatgg acgaccgggg catccccggc gatgtcaaga cccgcttgga gataaccgcc
      361 cggatggtca aggcgatgga ggggtgccgg gttccggtgg agcggatcta catcgacccg
      421 cttacccagc cggtctcgac cgatccgaag aacggtcgcg ttttcctgga gtccctgcgg
      481 gccataaagc gggagttccc gggtatcaag accacctgcg gcctctccaa cgtctccttc
      541 ggcctgccgg agcgccgggt gctcaaccgc tgctttctgg ccctggccat cgaggctggc
      601 ctcgattcgg ccctgatcga tccgaccgac cagggcatga tggccgccat gctgaccgcc
      661 gaggggctga ccgggatcga ccagtactgc atgagttacc tgaccgcctt ccgggaaggc
      721 aagatcaaaa gctgaagccg gttcgtaatt tttcgggttt tccccttgga gggggcgttc
      781 actgagttcg tctgaagaga gattcagaaa caaccgggcc gtgaagcaga gcgaagcacg
      841 cttcacggcg gtgaagtgtg ttgagggact ctacttcacc ggtgagagag ggggcggcag
      901 ccccttctcg tagtcgtatt cggggagagg gaatcagggg agggaagctc cttcccctga
      961 aagcgagccc cgaaactcgg cgcggcttga acaagtcaat atggatcggt tacatcgcgg
     1021 agtattgaaa atttgggaag tgttattgcg tttcggggcg tagcgcagcc tggtagcgca
     1081 cctgaatggg gttcaggtgg tcgctggttc gaatccagtc gccccgacca g
//
LOCUS       DAOQYN010000353         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_171003_length_1131_cov_2.298327, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000353 DAOQYN010000000
VERSION     DAOQYN010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171003_length_1131_cov_2.298327"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PKN80_09545"
     CDS             <1..472
                     /locus_tag="PKN80_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36290.1"
                     /translation="LAAGPALPGLPKTSTSELVGQARVYDGKPVLTQGEAIGDLMLRG
                     KEGWVNISDGNAALGIFGPAEELEKIRHLGRFQSLGDLVVVTGIFHDACPVHSGQMDL
                     HLLQLKVVAEGRRIPESFNFYKAGLAILLAIMAVGSLLLRQFVRKVTAGRPKRS"
     gene            complement(423..>1131)
                     /locus_tag="PKN80_09550"
     CDS             complement(423..>1131)
                     /locus_tag="PKN80_09550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ36291.1"
                     /translation="VNSYRDPETGTGFPGLLASIAGLDGLLRVGFLTSHPKDASEDLF
                     RTMAAEPRVYRHLHLPLQSGSDRILAAMNRRYTAAVFLDRVAAARGRVSELAVTSDLI
                     VGFPGERELDFQATLELLSAVRFDDLYAFKYSDRPGTRAQELGEKVAETVKAERLARV
                     WALQEQISLAAGLERVGREEEVLFLRRSSRRAGWLVGKSGRERTVLVEGGPELIGRLA
                     RVRIFSADRQLLYGRIA"
BASE COUNT          203 a          398 c          366 g          164 t
ORIGIN      
        1 cctggccgcc ggaccggccc tgcccggcct gcccaagaca agcacctcgg agctggtcgg
       61 ccaggcccgg gtttacgacg ggaaaccggt gcttacccag ggcgaggcga tcggcgacct
      121 gatgctccgg ggcaaggagg gttgggtgaa catcagcgac ggcaatgccg ccctcgggat
      181 cttcggaccc gccgaagaac tcgagaagat ccgccacctc ggccgcttcc agtcgctggg
      241 cgacctggtc gtggtgaccg gcatctttca cgacgcctgc ccggtccact ccggccagat
      301 ggacctgcac ctcctgcagc tgaaggtggt cgcggagggc aggcggatcc cggaaagctt
      361 caatttctac aaggcgggcc tggcgatact gctggcgatc atggccgtgg gcagcctgct
      421 gctcaggcaa ttcgtccgta aagtaactgc cggtcggccg aaaagatcct gacccgggcc
      481 aggcggccga tcagttccgg gccgccctcg accagcaccg tccgctcccg gccgctcttt
      541 ccaaccagcc agccagcccg acggctgctc cggcgcagga aaagcacctc ctcctcccgg
      601 cccacccgct ccaggccggc cgccagggag atctgctcct gaagcgccca gacccgggcc
      661 aggcgttccg ccttaaccgt ttcagccacc ttctccccca gttcctgcgc ccgcgtcccg
      721 ggccggtcgg aatatttgaa ggcataaaga tcgtcgaagc ggaccgccga aagaagttcg
      781 agcgtggcct gaaaatccaa ctcgcgctcg ccgggaaaac cgacgatcag atccgaagtg
      841 actgccagct ccgacacccg gccgcgggcg gccgccaccc tgtccaggaa gacggccgcg
      901 gtataacggc ggttcatggc cgccaggata cggtcggaac cggactggag cggcaggtgc
      961 aggtggcggt agacccgggg ctccgcggcc atcgtccgga acaggtcctc cgaggcgtcc
     1021 ttggggtggg aggtcaggaa gccgacccgg agcaggccat ccagcccggc tatggacgcc
     1081 agcagaccgg gaaacccggt cccggtctcc ggatctcggt acgagttcac g
//
LOCUS       DAOQYN010000354         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_178122_length_1104_cov_2.821735, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000354 DAOQYN010000000
VERSION     DAOQYN010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178122_length_1104_cov_2.821735"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKN80_09555"
     CDS             <1..200
                     /locus_tag="PKN80_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36292.1"
                     /translation="HGAVALGVTLKEAYFRSLLLENAAAFLLAILAAGGRPRYLTPKE
                     IRSIECLEAEDYRKLLLQKGV"
     gene            267..518
                     /locus_tag="PKN80_09560"
     CDS             267..518
                     /locus_tag="PKN80_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36293.1"
                     /translation="MNCFPMNCYVSVCLGIITLFMLVTGILAIKVLLRLKKLADEIEE
                     VVHKARRVVGLADPLFTIVNRVYGIVHLFSRKREAGKNG"
     gene            511..747
                     /locus_tag="PKN80_09565"
     CDS             511..747
                     /locus_tag="PKN80_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNQ36294.1"
                     /translation="MAEENGEMKGLLLGLALGTVLGAVLGVILAPASGKETRGRIKDV
                     WDTLPEKTRRTVDKIREKLQKKEDEGLPEGEARG"
BASE COUNT          217 a          302 c          384 g          201 t
ORIGIN      
        1 accacggcgc ggtggcgctc ggggtaaccc tcaaggaggc ctacttccgg agcctgctcc
       61 tggagaatgc cgccgccttc ctgctggcca tcctggccgc cggcggccgg ccccgttacc
      121 tgaccccgaa ggagatcagg tcgatcgagt gcctggaggc cgaggattac cggaagctcc
      181 tgctgcagaa aggggtttga ggccggtccc ccggctcgct tgacaccggg tggccctttg
      241 gctaaaattg ttgacgggag gtttccatga actgttttcc catgaactgt tacgtatccg
      301 tctgcctcgg catcatcacc ctcttcatgc tggtgaccgg catcctggcg atcaaggtgc
      361 tgctcaggct taagaaactg gccgatgaaa tcgaggaagt ggtccacaag gcgcgccggg
      421 tcgtcggcct ggccgacccg ctcttcacca tcgtaaacag agtctacgga atcgttcatc
      481 ttttctcaag gaaaagggag gctgggaaaa atggctgaag aaaatggcga aatgaaaggt
      541 cttctgctcg ggctggcgct cggaacggtc ctgggcgcgg tcctgggcgt gatactggcc
      601 ccggcctccg gcaaggagac ccgcggccgg atcaaggatg tctgggatac gcttcccgag
      661 aagacgcgcc ggacggtgga caagatccgg gagaagctcc agaagaagga ggacgaaggc
      721 ctgccggagg gagaagcccg gggctgagtc ggaaagaggc tgattttcag gggccgccgg
      781 attcgagggt tgaatccggc ggcctttttt catgtctttt gtggtaaata tcgtccttaa
      841 gggtaaaaat ggcttatttt tgatccgcgg gctgaaaaac ggccttttga gccggccggg
      901 aaaggagaat ggaggggctg aaggcggccc ggagccggtc agccgggcgc gagcggcgcg
      961 gccggccagg gccggcggcg gctggaagcc ggcgggcggc ggaaggaatc agacggcggc
     1021 tggcctgacg aaccgggagg catccttttc agagccggag aatcgaccgg cggaatccga
     1081 ccgccggttc cggccatgag tttg
//
LOCUS       DAOQYN010000355         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_178926_length_1101_cov_3.768642, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000355 DAOQYN010000000
VERSION     DAOQYN010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178926_length_1101_cov_3.768642"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PKN80_09570"
     CDS             <1..693
                     /locus_tag="PKN80_09570"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNQ36295.1"
                     /translation="MRANHIENPDLIRIARLLIVPSRMILPAAPENGLVVNLPEYRLF
                     FFRAGKLLAIYPVGIGRTDWQTPLGAFKVTSRVRNPSWRMTENIARRENNGYREIVPP
                     GPDNPLGDYWVGTSIESTGLHGTNQPGSIGLAMSHGCVRLAPGQVGFFFSQVRIGDPG
                     EFIYEPVKAAAWGGRILIEAHPDVYGLCPDFEARARARLEGLGLWERVDPERLRRVLA
                     ERRGVPTPVEKR"
     gene            732..>1101
                     /locus_tag="PKN80_09575"
     CDS             732..>1101
                     /locus_tag="PKN80_09575"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNQ36296.1"
                     /translation="MNTKWLAINHDFLRIELAQLKDEGRDTADLESEFQAWLVRSPEE
                     LEAAQPALNALLDRAAARPFRPEAEGREPSDLASIRSLRSAPVSLPPASGDAKGRADR
                     LLGAWLGRCCGCLLGKPVEGW"
BASE COUNT          169 a          382 c          375 g          175 t
ORIGIN      
        1 atgcgggcca accacattga aaatccggac ctgatccgga tcgcccggct gctgatcgtt
       61 ccgagccgga tgatccttcc cgcggcgccc gagaacgggc tggtggtcaa cctgcccgag
      121 taccggctct tcttcttccg cgccggcaaa ctgctggcca tctacccggt cggcatcggc
      181 cggaccgact ggcaaactcc gctgggcgcc ttcaaggtca ccagccgggt gcgcaacccg
      241 tcctggcgca tgaccgagaa tatcgcccgc cgcgagaata acggttatcg cgaaatcgtg
      301 ccgcccgggc cggacaaccc gctgggcgac tactgggtgg gcacctcgat tgaaagcacc
      361 ggtctgcacg gcaccaacca gcccggttcc atcggcctgg ccatgagcca cggctgcgtc
      421 cggcttgcgc cggggcaggt cggtttcttc ttcagccagg tcaggatcgg cgacccgggc
      481 gaatttatct acgaaccggt caaggcggcc gcctggggcg gccggatcct gatcgaggcc
      541 cacccggacg tttacggcct ctgccccgac tttgaggccc gggcccgggc gcggctggaa
      601 gggctcggcc tctgggagcg cgtcgacccg gaacgcctgc ggcgcgtgct ggccgagcgg
      661 cggggtgtgc cgacgccggt cgaaaagaga tgagataggt tgctgtttat cgccatcggg
      721 agggaaaggg catgaatacc aagtggctgg cgatcaacca tgatttcctg cggatcgagc
      781 tggcccagtt gaaggacgag gggcgcgaca ccgccgacct ggaatccgaa ttccaggcct
      841 ggctggtccg ctcccctgag gagcttgagg cggcccagcc cgccttgaac gccctgcttg
      901 accgggcggc cgcccgtccc ttccggccgg aggcagaagg ccgggagccc tcggacctgg
      961 cctccatcag gtcgctccgg tcggccccgg tctccctgcc gccggcctcc ggggatgcca
     1021 aaggccgggc cgaccgcctc ctgggcgcct ggctcggccg ctgctgcggc tgcctgctcg
     1081 gcaagccggt cgagggctgg c
//
LOCUS       DAOQYN010000356         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_184486_length_1082_cov_2.017527, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000356 DAOQYN010000000
VERSION     DAOQYN010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184486_length_1082_cov_2.017527"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PKN80_09580"
     CDS             complement(<1..907)
                     /locus_tag="PKN80_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNQ36297.1"
                     /translation="MKIILDAAVSGPTPAGLEVWCRKLLEALAGVDQENQYLVWGAFW
                     RDFSKRRKRIYLPAAPNFRLKVWRMPRRLATGLEDRGRPLLETGLAADQADIFHGLRY
                     FLPRLRRLKGVATIYDLSFVINPGWYQDAWYRNVPLYCRRADAIAAISRTTADDLVKH
                     FGLPAKKIEVIYGGVGRPFRPESDRNRLEAFRREQGLERPFLLTVATSDRRKNLAGLL
                     EAFGLAAERIPELELVIAGNPAAVQPPLRERINRLGLADRIRFPGYLPTTDLVRLYNL
                     ALAFVFPSFYEGFGLPVLEAMACGTP"
     gene            complement(929..1078)
                     /locus_tag="PKN80_09585"
     CDS             complement(929..1078)
                     /locus_tag="PKN80_09585"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNQ36298.1"
                     /translation="MRPSEVHELRGNIARARKKLGWQPKVKFEKLVRIMVESDLKELR
                     KEHNL"
BASE COUNT          175 a          340 c          381 g          186 t
ORIGIN      
        1 ccggcgtgcc gcaggccatc gcctccagca ccggcaggcc gaagccctcg tagaatgatg
       61 ggaagacaaa ggccagggcc aggttgtaga ggcggaccag gtcggtggtc ggcaggtaac
      121 cgggaaaccg gattcgatcc gccaggccga gccggttgat gcgttcgcgc agaggcggtt
      181 ggaccgcggc cgggttgccg gcgatgacca gctccagttc cggaatacgt tcggcggcca
      241 ggccgaaggc ctccagcagg ccggccaggt tcttccggcg gtcggaggtg gccacggtga
      301 gcaggaaggg gcgttccagg ccctgttccc ggcgaaaggc ctccagccgg ttccgatcgg
      361 actcgggccg gaagggacgc cccaccccgc cgtagatcac ctcgatcttt ttcgccggga
      421 ggccgaagtg ttttaccagg tcgtcggcgg tggtgcgcga gatggcggcg atcgcgtcgg
      481 cccgccggca gtagaggggc acgttgcggt accaggcgtc ctggtaccag cccgggttga
      541 tcacaaagga gaggtcgtag atggtggcca cgcccttcag ccggcgcagc cggggcagaa
      601 agtaacggag gccgtggaag atgtcggcct ggtcggcggc cagcccggtt tccaggagcg
      661 gccggccgcg gtcttccaga ccggtcgcca gccgccgcgg catccgccac accttgagcc
      721 ggaagttggg cgccgccggc aggtaaatcc gcttccggcg tttggaaaag tcgcgccaga
      781 aggcgcccca gaccaggtac tggttctctt ggtcgacccc ggccagggcc tccagaagct
      841 tccggcacca gacctccagt ccggccggcg tcgggccgct tacggccgcg tcaaggatta
      901 tcttcattcg cggctttccg ggtccgggct aaaggttgtg ctccttgcgg agttccttca
      961 ggtcggattc gaccatgatc cggaccagtt tttcgaattt gaccttcggt tgccagccca
     1021 gtttttttcg ggcccgggcg atatttcccc gaagttcgtg gacttcggac ggccgcagga
     1081 gc
//
LOCUS       DAOQYN010000357         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_188581_length_1068_cov_2.816387, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000357 DAOQYN010000000
VERSION     DAOQYN010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188581_length_1068_cov_2.816387"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1068
                     /locus_tag="PKN80_09590"
     CDS             <1..>1068
                     /locus_tag="PKN80_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ36299.1"
                     /translation="GAGPAGLAAAYFLARLGYRPRVFEAEPRPGGMMVQTIPAYRLPR
                     EVVAREVRMIESLGVQIDTGVRLGTDFSLEDLRNQGYEAVFLGIGAPDGVKLDIPGET
                     AEGVTDAIQLLRRYNLTGSVPIGRRVAIIGGGNAAIDAARSAVRLGAESVTIVYRRTR
                     EEMPAYAEEVEEALEEGVKLELLAAPKEILQKHGRVTGVRCSRMELGEFDRTGRRGPR
                     TRKDEDFTVEADQVVVAVGQRINLPGVFEKLDLKLDRRGFVEVNPATGQTSTPWLFAG
                     GDAVYGPSSVVEAIAAGERPAVGIDAYLSGAEHAFWRDMKEVDTLFDPEADPVDYGRA
                     GIERVATLKRKYNFSEVELPW"
BASE COUNT          174 a          342 c          398 g          154 t
ORIGIN      
        1 cggcgccggt ccggccgggc tggcggccgc ctacttcctg gcccggcttg gctaccggcc
       61 ccgggtcttc gaggccgaac cccggcccgg cggcatgatg gtccagacga tcccggccta
      121 ccggctgccc cgggaggtgg tcgcccggga ggtgcggatg atcgaaagcc tcggggtgca
      181 gatcgacacc ggggtccggc tgggcaccga tttcagcctg gaggacctgc gcaaccaggg
      241 ctatgaagcg gtcttcctgg gcatcggcgc cccggatggg gtcaagctcg acatcccggg
      301 cgagacggcc gagggagtga ccgatgccat tcaactcctg cgccggtaca atcttaccgg
      361 cagcgttccg atcggccggc gggtggcgat catcggcggc ggcaacgcgg ccattgacgc
      421 ggcccggtcg gcggtccggc tgggcgccga gtcggttacc atcgtctacc ggcgcacccg
      481 cgaagagatg cccgcctacg ccgaggaggt cgaggaggcc ctggaggaag gcgtcaagct
      541 ggaactcctg gccgcgccca aggagattct ccagaagcac ggccgggtga ccggcgtgcg
      601 ctgcagccgg atggaactgg gcgagttcga ccggaccggc cgccgcgggc cacggacaag
      661 gaaggacgag gatttcaccg tcgaggccga ccaggtggtg gtggcggtcg gacagcggat
      721 caacctgccc ggcgtctttg aaaaactcga cctgaaactg gaccggcgcg gcttcgtgga
      781 agtgaacccg gccaccggcc agacctccac gccgtggctg ttcgccggag gggacgccgt
      841 ttacgggccg tcctcggtgg tggaggcgat cgccgccggc gagcggccgg cggtcggcat
      901 cgacgcttac ctgagcgggg ccgagcatgc cttctggcgg gacatgaagg aggtcgacac
      961 cctctttgat cccgaggccg acccggttga ttacggccgg gccgggatcg aaagggtggc
     1021 cacgctgaag cgcaagtata atttcagcga ggtggaactg ccctggag
//
LOCUS       DAOQYN010000358         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_189459_length_1065_cov_3.412871, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000358 DAOQYN010000000
VERSION     DAOQYN010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189459_length_1065_cov_3.412871"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PKN80_09595"
     CDS             <1..690
                     /locus_tag="PKN80_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNQ36300.1"
                     /translation="AVKSPAEIDLLRRGFRISERAVARVIAELAPGRTELEMVGTAQA
                     AIYQGGGEYEAHPVYVLAGRHSTHAIGRPTHRRIRSGEVVQLNIGARVAGYSPSVGRP
                     VFMGRITPEARRLFQQGLDLHRRTIELMKAGVAARSVVEKFYDYAGKLGCRQNLLYGP
                     CHGLGLIEVERPWMEETSDYRLRERMTFQVDTFLYNRDFGLRWEDGVVVTRDGVEELS
                     SRLQRIIQVRG"
     gene            complement(783..>1065)
                     /locus_tag="PKN80_09600"
     CDS             complement(783..>1065)
                     /locus_tag="PKN80_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNQ36301.1"
                     /translation="ELTPLTRNILKKNAEIIKASSGVKFLLEATCSPEGSIEYNLKLA
                     ERRVKAVYDYLVGLGVGKDLLMLKSLGEIEVEQVSYPFARKVHFETLVK"
BASE COUNT          171 a          338 c          368 g          188 t
ORIGIN      
        1 gccgtcaaga gcccggccga gatcgacctg ctgcggcgcg gtttccggat cagcgaacgg
       61 gcggtggccc gggtgatcgc cgagctggct ccgggccgga ccgagctgga gatggtaggc
      121 acggcccagg cggccatcta ccagggcggc ggcgagtacg aggcccaccc ggtttacgtg
      181 ttggccggcc gccattccac ccacgccatc ggccgcccca cccaccgccg gatcaggtcc
      241 ggcgaggtcg tccagctgaa catcggcgcc cgggtggccg gttattcgcc ctcggtcggc
      301 cggccggtct tcatggggcg gatcacgccg gaggcgcgcc gcctctttca gcaggggctg
      361 gacctgcacc gcaggacgat cgagctgatg aaggccgggg tggcggcccg gtcggtggtg
      421 gagaagttct acgattacgc cgggaagctc ggctgccgcc agaacctgct ctacgggccc
      481 tgccacggcc tgggcctgat cgaggtggag cgcccctgga tggaggagac ctccgactac
      541 cggctccggg agcggatgac cttccaggtg gacaccttcc tctataaccg ggatttcggt
      601 ctccgctggg aagacggggt ggttgttacc cgggatggcg tggaggagct atccagccgg
      661 ttgcagcgga tcatccaggt caggggatag gcaatcggcc cgaaccaacg gtcacaaaaa
      721 aagccccggc cgggttctcc ggccggggct cctttttgat gatgggaggc gcgtcggcag
      781 ccttacttga ccagcgtttc gaaatggacc ttccgggcga agggatagga gacctgctcc
      841 acctcgatct cgcccaggct cttgagcatg agcaggtcct tgccgacgcc cagtccgacc
      901 aggtaatcgt agacggcctt gacccggcgc tcggccagtt tcaggttgta ctcgatgctc
      961 ccttccggcg agcaggtggc ctcgagcagg aacttcacgc cgctggaggc cttgatgatc
     1021 tcggcattct tcttcaggat gttcctggtc agcggggtca attcg
//
LOCUS       DAOQYN010000359         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_207600_length_1011_cov_3.544979, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000359 DAOQYN010000000
VERSION     DAOQYN010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207600_length_1011_cov_3.544979"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PKN80_09605"
     CDS             <1..927
                     /locus_tag="PKN80_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HNQ36302.1"
                     /translation="IRPKLARLMDGFLEDFPAVPVRPAVAWPAEVPVIDWERLRRRLR
                     LDAGVFGSGRFRPGGRAGQLVMREFLRDRLDRYPVDRNDPNAGGQSNLSPYLNHGQLS
                     AQRVVLETLEAGRASKASEVFLEELVTRRELAENYCLYNPDYDRPEGFPEWARRSLEK
                     HRADRRRYLYRRPELEAAETHDPIWNTAQRQLAGLGKLHGYLRMYWAKKILEWSPDPE
                     AAQAEAVYLNDRYALDGRDPNGYAGIAWSIGGVHDRPWFERPVYGQVRYMNESGCRRK
                     FNVDRYLESGKAIPGPASRLVAGSPADGRKSG"
     gene            924..>1011
                     /locus_tag="PKN80_09610"
     CDS             924..>1011
                     /locus_tag="PKN80_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012194017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNQ36303.1"
                     /translation="MRTIILLTISNIFMTFAWYGHLKYKNSPL"
BASE COUNT          175 a          339 c          326 g          171 t
ORIGIN      
        1 atccggccca agctggcccg cctgatggac gggttcctgg aggatttccc ggccgttccg
       61 gtccgcccgg cggttgcctg gccggccgaa gtcccggtta tcgactggga gcgcctgcgc
      121 cgccgcctcc ggcttgacgc cggcgtgttc ggttcgggcc gtttccgccc cggcggccgg
      181 gccggacaac tggtcatgcg ggaattcctg cgcgaccggc tggaccgcta cccggttgac
      241 cgcaacgatc ccaacgccgg cggccagtcg aacctctccc cctaccttaa ccacgggcag
      301 ctttcggccc agcgggtggt gctcgagacg ctggaagccg gccgggcctc gaaggccagc
      361 gaggtcttcc tggaggaact ggtgacccgg cgggagctgg ccgaaaatta ctgcctgtac
      421 aatcccgatt acgatcgccc ggaaggtttt ccggaatggg cgcgccggag cctggagaag
      481 caccgcgccg accgccgccg ctatctttac cggcgtccgg agctggaggc ggctgagacc
      541 cacgacccga tctggaacac cgcccagcgg caactcgccg ggctgggcaa gctgcatggt
      601 tacctgcgca tgtactgggc gaaaaaaata ctggagtggt ccccggaccc ggaggccgcc
      661 caggccgagg ccgtctacct taacgaccgt tacgccctgg acggccgcga tcccaacggc
      721 tacgccggaa tcgcctggtc gatcggaggg gttcacgacc ggccctggtt tgaacggccg
      781 gtttacggcc aggtaaggta catgaacgag tccggctgcc ggagaaaatt caacgttgac
      841 cggtacctgg agtccgggaa ggcaatcccc ggtccggctt cacgcctggt tgccggttcg
      901 ccagcggacg ggaggaaatc cggatgagga ccattatcct gctgaccata tccaacattt
      961 tcatgacctt cgcctggtac ggccacctca agtacaagaa ttcgccgctc t
//
LOCUS       DAOQYN010000360         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1009
            NODE_209742_length_1005_cov_3.458947, whole genome shotgun
            sequence.
ACCESSION   DAOQYN010000360 DAOQYN010000000
VERSION     DAOQYN010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852352
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:04:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,882
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209742_length_1005_cov_3.458947"
                     /isolate="AMR_MDS_1009"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKN80_09615"
     CDS             <1..846
                     /locus_tag="PKN80_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610104.1"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and IscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     in Thermotoga this enzyme has an extra C-terminal domain;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNQ36304.1"
                     /translation="QVFQETYHRQTYASVHPAGSLKSDYRWRLYSMHRSLEAGIDDVG
                     LGALFGLYDWRFEVLGLLYHARALEKRFGIGPHTISFPRLEPAENTPFIQETGYRVTD
                     EDFRKAVTVIRLSVPYAGMILTAREPAPLRRALLPLGCTQLDASSRIGIGAYRSRSGE
                     QVSDRQQFLLGDTRTLDEVVRELALMGHITSFCTAGYRCGRTGQCIMELLRSGQEGRF
                     CKLNAALTFQEWLDDFATPPTRAAGEPILAREIEDIRQRMPAVFPAFMKQYERIRQGE
                     RDLYF"
     gene            824..>1005
                     /locus_tag="PKN80_09620"
     CDS             824..>1005
                     /locus_tag="PKN80_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ36305.1"
                     /translation="MNEISTSELIKRFQRLAGRLESKHAVLLQLALVRGRRWSYLAGR
                     MPAPGLSPVSERVKLGP"
BASE COUNT          156 a          353 c          318 g          178 t
ORIGIN      
        1 caggttttcc aggagaccta tcaccggcag acttacgcca gcgtgcatcc ggccggcagc
       61 ctcaagtccg actaccgctg gcgcctctac tcgatgcacc gctccctgga ggccggcatc
      121 gacgacgtcg gactgggcgc cctcttcggg ctttacgact ggcggttcga ggtgctcggc
      181 ctgctttatc acgcccgcgc cctggagaaa aggttcggga tcggtcccca caccatctcc
      241 ttcccccggc tggagccggc cgagaatacc ccgttcatcc aggagaccgg ctaccgggtc
      301 accgacgagg atttccggaa ggcggtaacc gtcatacgac tctcggtgcc ttacgcgggc
      361 atgatcctga ccgcccgcga accggccccg ctccgccggg ccctgcttcc cctgggttgc
      421 acccagctgg acgcctcctc ccggatcggg atcggcgctt accgttccag atccggggag
      481 caggtttccg accggcagca gttcctgctg ggcgacaccc gcaccctgga cgaggtggtg
      541 cgggagctgg ccctgatggg ccacatcacc tctttctgca ccgccggata ccgctgcggc
      601 cggaccgggc agtgcatcat ggaactgctt cgcagcgggc aggaggggcg tttctgcaag
      661 ctgaatgcgg cgctgacctt ccaggagtgg ctggatgatt tcgccactcc gccgacccgg
      721 gcggccggcg aaccgatcct ggcccgggag atcgaggata tccggcagcg gatgccggcg
      781 gtcttcccgg ccttcatgaa acaatacgag cgtatccgac agggtgaacg agatctctac
      841 ttctgaactt atcaagcggt ttcagcgcct ggccggccgc ctggaaagca agcacgcggt
      901 gctcctccag ctggctctcg tgcgcggccg gcgctggtcc tacctggccg gccggatgcc
      961 ggccccgggc ctctccccgg tctccgaacg ggttaagctg gggcc
//
